LOCUS       DAOSRP010000001        42058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1037_length_42058_cov_5.622336, whole genome shotgun sequence.
ACCESSION   DAOSRP010000001 DAOSRP010000000
VERSION     DAOSRP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 42058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42058
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1037_length_42058_cov_5.622336"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..685)
                     /locus_tag="PLJ82_00005"
     CDS             complement(95..685)
                     /locus_tag="PLJ82_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR39930.1"
                     /translation="MCNREQKITKEKQKFPAIFLDRDGTLIEDVGYIKEPSAVSFFPN
                     TFADLIRLQEHFMLFIITNQSGIGNNLLTEKEVKIVNSYIETELKKQGITITATYYCP
                     HTTEDNCFCKKPSPYFINQAVHEYNIDRKHSYIIGDHPSDSECGIRAGLTPIYVLTGH
                     GNKHKNELTCEVAICKSLSHATERILNNIYYDNKII"
     gene            complement(753..1304)
                     /locus_tag="PLJ82_00010"
     CDS             complement(753..1304)
                     /locus_tag="PLJ82_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR39931.1"
                     /translation="MKRIGERIKKYRRIYNMALGDLATKVGITPSALSQIENAKAFPS
                     IITLKSIAENLHTTVGELIGENEASKHSPVYRAVDYKLLDNLDYKATIYHIPYEDVNK
                     QMDSFVIILKPQEKVENIFASHSGQRMCYVLQGNIEFTIDEKKVELKTGDSAFFHAKN
                     ISILQNKQQEDTKLLLVIAPASM"
     gene            complement(1681..2313)
                     /locus_tag="PLJ82_00015"
                     /pseudo
     CDS             complement(1681..2313)
                     /locus_tag="PLJ82_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            complement(2411..3397)
                     /locus_tag="PLJ82_00020"
     CDS             complement(2411..3397)
                     /locus_tag="PLJ82_00020"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOR39932.1"
                     /translation="MKIACFSTKSYDKEYLSKAIVAPHSIVFFEESLNPNTVGLLTGF
                     DGICVFVNDKLDNNLLQQIASAGIKAIFLRCAGFNNVVISTATSLGIKVYRVPAYSPY
                     AVAEHAVALVLTLNRKTHKAYNRVKEYNFSIERLHGFDVHGKTVGVIGTGTIGLVFAK
                     IMLGFGCKVIAFDPTESDDAKQLGIQYVALEDVLKQADILSLHCPLNQHTRHMISTKE
                     FSIMKHGAMLINTSRGALIDTKEAIEALKTGALGYLGIDVYEEEEKLFFHDFSESIIS
                     DDILMRLMTFPNVLITSHQAFFTHEALTQIAETTYQNMLDFTHGTSSTNQLL"
     gene            complement(3459..4457)
                     /locus_tag="PLJ82_00025"
     CDS             complement(3459..4457)
                     /locus_tag="PLJ82_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR39933.1"
                     /translation="MNKIIILIISCISLTSCENYFFSADKSSSNPYDNFEYLWNELDK
                     KYSYFELKNIDWDEMKTTYEARLYPNMSETELFNTLADLMNELRDDHSNLVSPFNVSV
                     YNVPLTKPANYHARTIDEFYIPHAERTGSFKHAMLSGTNIAYIRYASFSDDISNTHLD
                     YLLDKYRYTDGIILDLRDNGGGSMFNIPMLLERFIDSRTLVAYTLTRNGKGRNDFSDR
                     ATFYMNPYEGTRYTKPIMVLIDRGSYSATTFFALSTKAIPNLTLVGDTTGGGGGMPNG
                     GQLPNGWTYRFSISQLLDLDGNNYAEDGVPPDILVSFDWTDLTKDEIIERAIQEID"
     gene            complement(4465..5346)
                     /locus_tag="PLJ82_00030"
     CDS             complement(4465..5346)
                     /locus_tag="PLJ82_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR39934.1"
                     /translation="MKKRLIIILFLIISKALVAIANEEVVKKSSQWENYFTPSVGIEL
                     IKFRDLATSPLFYSGAGINGNISWLWIHPKNETYTELSITSANTAAYMPLSNILQGYS
                     GSTFMSGSIYLHTLFTVPFNYDDKLTIKAGGAITNTWNIRSNPSLFNNGFGMESFANL
                     SLSTKGIYDVSRKNIVIKDYYFFTRTLFPAKRQIGFQLNIGLLNLNYRPGYAYNADIA
                     LDGTNTNPLAFLLDGHKWTINGYRLGTRLYWEQFTPNGNGHRWSYIWDIAQAPGRFEP
                     FEMATHKIQYTLMFRNK"
     gene            complement(5639..5932)
                     /locus_tag="PLJ82_00035"
     CDS             complement(5639..5932)
                     /locus_tag="PLJ82_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HOR39935.1"
                     /translation="MKNFILKLALICAVCLVMTSCYTYTYTVGNGPQTGVEITKKNHY
                     LLYGLAPISTSNPVEMAGGAADYEVTIQHTFVDGLLNALTSGLYTPTTTIVRK"
     gene            complement(6192..6485)
                     /locus_tag="PLJ82_00040"
     CDS             complement(6192..6485)
                     /locus_tag="PLJ82_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor protein"
                     /protein_id="HOR39936.1"
                     /translation="MKKFILKAALICGVCLVMTSCYTYTYTVGNGPQTGVEVTKKNHY
                     LIAGLVPISTSNPTEMAGGAANYEVTIQHTFVDGLLNGLTGGLYSPTTTKVRK"
     gene            complement(6574..7893)
                     /locus_tag="PLJ82_00045"
     CDS             complement(6574..7893)
                     /locus_tag="PLJ82_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOR39937.1"
                     /translation="MNILLKKLVSHVTIIPELDEFDAIHSSIVKDIAFKGTNLWILMF
                     AIIVASVGLNMNSTAVVIGAMLISPLMGPINGMGYSIATYDIALFKRAVKNFSFAVIA
                     SLVASTIYFTLSPISTAHSELLARTSPSIYDVLIALFGGLAGIIAISSKQKGNVIPGV
                     AIATALMPPLCTAGYGLATFQMNFFFGAIYLFTINTVFIALASLFVSQLLKFPIRTLV
                     DERKKRRVNRYISTVIAIVFLPSIYFGYTLVVKEKFTENATKYISNVSLLDDIYLLKS
                     DIDTKNESISLVYGGSSMDDETRQSIIDKAKDFNLDKAQILVRQGFTFDGAEEHNAEL
                     YKLRLELSHVDTQLAIKQQKIDSIQQQHEMGKIILKEIRTMYPQIQACTFTQGFSHHI
                     NKEKEAQQTQIILFSLEGDTISSDNKSTIKNWLKTRLQEDQIRVYYN"
     gene            complement(7905..10022)
                     /locus_tag="PLJ82_00050"
     CDS             complement(7905..10022)
                     /locus_tag="PLJ82_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR39938.1"
                     /translation="MILATIFETTLPLTNPVLKFLLILIIILAAPIFLNKIKIPHLLG
                     LIIAGAIIGPFGLNLIERDSGIILSGTAGLLYIMFLAGLEIDIADFKKNTSRSLIFGM
                     YTFIIPMVLGILGSIFILQFSITTSVLLASMFASHTLIAYPLISKLGVVKNKAVTMAV
                     GGTMITDTLALLVLAVIVGMANGEVNSDFWIRLSVSLLAFGLIITILFPIIARWFFKK
                     FDDNVAQYIFVLAMVFLGATLAELAGIEAIIGAFLTGLALNRLIPHTSPLMNRVEFVG
                     NAIFIPFFLIGVGMLVDYRAFFKDWETLKVAAVMTIIATLAKFLAAWLTQKTYKLSLD
                     ERRIIFGLSNAQAAATLAAVLIGYNLQIFDASVLNGTILMILVTCTIASFQAQKGAKN
                     IALSEDLKSSTGEVETDERILIAINQENNLEELVNLGVTIKSKHNKKGLYALHIIDND
                     ESQNNNEKQAKKLLQQAGRIAAATDTKLNELLRYDINLVNGITSSLKEQRITDLILGL
                     NNEKGISDGFFSNLTLGILSKSNPTTLIYKPTQPFGTIKRHLVIVPDKAEKEIGFPFW
                     IIKTWNIARNSGAKIVFYSSIDTQKVIREIQSKHPIECTFKEFSDWDDFLILSRDIEA
                     NDNLIVILSRKDKPSYTPSMQRIPSYLNKYFEGNSYILVYPMQVGVGETSMMDISNPT
                     ILEPIEKLDEIGKTIAKIFRKKI"
     gene            complement(10113..11429)
                     /locus_tag="PLJ82_00055"
     CDS             complement(10113..11429)
                     /locus_tag="PLJ82_00055"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOR39939.1"
                     /translation="MQKEGQDIQVFWFRRDLRLTDNVGLYNALQDKRYPVFPLFIFDT
                     DILSSLPSTYDKRVDYIHQILTSLNQELHQLGSSLMVEIGKPTEVFEKLTTQFTIKAV
                     HTNSDYEPYAIQRDKEVRSLLHTKGITLHTYTDQVIFAPGEVIKADGSPYTIFTPFSK
                     IWLRKYEQLPHQEYTTDAYKKNWLSTPAQEIPSLAKCGFKKTSIQYTPPSIPYSIIDN
                     YTDTRNFPAINGTSTLSIHLRFGTISIRQLARIAFTRNHTYVSELIWREFFMQILYFF
                     PHVQTNAFKPKYDAIEWRNDTAEFSRWCNGTTGYPLVDAGMRQLNETGWMHNRVRMVV
                     ASFLCKHLLVDWRWGEAYFATKLMDYDLSANNGNWQWAAGCGCDAAPYFRVFNPTEQA
                     KKFDSNSEYIRQWIPEVDTNKYATPIVEHTFARNRAIETYKKAISG"
     gene            complement(11432..11974)
                     /locus_tag="PLJ82_00060"
     CDS             complement(11432..11974)
                     /locus_tag="PLJ82_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOR39940.1"
                     /translation="MTNYMNPWHHVSPHTTQTDSVQAIIEIPKGSRAKYELDKESGLI
                     KLDRVLYSSVYYPVNYGFIPQSYCNDKDPLDILVLSMIELTPMCIVTAKVIGVMQMVD
                     SGATDNKIIAVATGDPSVSHYNDIEELPPHFISELRNFFEDYKKLEKKHVEVDEFLNK
                     KAALEIVAESFVYYDTTFRK"
     gene            complement(11996..12301)
                     /locus_tag="PLJ82_00065"
     CDS             complement(11996..12301)
                     /locus_tag="PLJ82_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOR39941.1"
                     /translation="MTGTKKISYIEQQQRTIKGMIAYYCKKKHQQGQLCEECKTLTAY
                     ALARLSACKYGENKPSCRKCPTHCYAPNKRKMMEERMRVVGPKMVYLRYFLSAYENY"
     gene            12541..15246
                     /locus_tag="PLJ82_00070"
     CDS             12541..15246
                     /locus_tag="PLJ82_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOR39942.1"
                     /translation="MNKIVKRILVSFLVLFILVFATLLTAPYFFKDKILTLAKQEVNA
                     LLNARADFSGIKLSFIRNFPNATVQLQDFSIVGVADFEADTLFYAKDFGMVVNLKSLF
                     ADNGYDVKRIEVDEASVKLRILPDGRANWDIMKEDTLAVEDTSAFAFHFKLQDFTIKR
                     TNFSYIDEEGDMQLYLTNINHHTTGDLTADSSLLVTKTTAETVTFVMEGMEYLSKATA
                     VFDATINANLNDMKFTFSENQSQLNAVPFAFDGWLKSIDNGWAMDITLLASEVDFKSL
                     LSLVPAVYATDFASLQADGKVSLTGFVKGDMVGDYYPAFGLQLAVENAWFKYPDLPKK
                     LDQITIKTAITNPGKTLDETVIDVSRFAFRMGNNPFAASMHITTPMSDPNVDFKANGK
                     LNLSDIQEFYPLEQGITLQGLLDVDMLLAGRMSYYEKNQYDKFTFAGKIDITKMHLVY
                     PSLPQALKINKASALINNRFVELSTLDMQIGRNDIQAQGKLENVVPYVLKNQTLDGQL
                     ALQSRYFNASDFMPQDSVQEETDEEPMSVIVLPKNIHFVCTATFDELVYEKMNFTNAN
                     GKLTIADGDLTINNMSVNAFGGKMLMNGVYSTTEPEKPRVDFTLQIQDVLFSEVSKQV
                     ETLSKFAPILGNALGKFSTTFSFNSLLQQDMMPDLASFIGSGKLTTSSVGLENVPALN
                     AIAKSLKRDDIVPMVVKDIAMMFEINNGKVTTKPFKFKVKDVDFTLGGQTGLDKSIAY
                     TGNVRLPDKLNLGKFSNMAIAVGGTFTKPTVKVDLLSTLGAVVEEKKQEIKAQVTEKV
                     DDAKEKALEDARLRRAKALEQANKEADKLVQEAKVAGERLVAEADKKGKALVAQANNP
                     LTKRAAEVSAREMVKEAQKQADNLNKRAEEEAKRILLKAEQSSTF"
     gene            complement(15669..16142)
                     /gene="rlmH"
                     /locus_tag="PLJ82_00075"
     CDS             complement(15669..16142)
                     /gene="rlmH"
                     /locus_tag="PLJ82_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOR39943.1"
                     /translation="MRIVLLVIGKTTNTYLQNLITDYQNRLKHYVSFDLQVIPELKNA
                     KHLSIEMQKEKEGECILKQLEDANDIILLDEKGTQFSSVDFAQHIDKAQVNGKKKILF
                     VIGGPYGFSEQIYQKATQKISLSKMTFSHQMIRLIFVEQLYRAFTILNNEPYHHE"
     gene            16206..16619
                     /locus_tag="PLJ82_00080"
     CDS             16206..16619
                     /locus_tag="PLJ82_00080"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOR39944.1"
                     /translation="MKKTKYIYVLLPLMFFFIGGGVSVAQQEMIDRISEGFVRGNVSF
                     VSPYFNDRMELSILGQHIGVYTKLQSQEIVQAFLTNNTPQSFNISHKGIKENSGFAIG
                     ILTTTNNKKYRVYILVRNTNEKSLIHQLRIDETND"
     gene            16612..17451
                     /gene="nadC"
                     /locus_tag="PLJ82_00085"
     CDS             16612..17451
                     /gene="nadC"
                     /locus_tag="PLJ82_00085"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOR39945.1"
                     /translation="MINDIAYLLKEWFSEDIGDGDHSSFSCIPSNAMGTSRLLVKEDG
                     VIAGVEVAKQVFAHFDNTLQVTVFIPDGTKVKKGDIVFDVTGNVRSLLQTERLMLNLM
                     QRMSGIASVTRVYVDELQGLATKVLDTRKTTPGLRFLEKMAVKIGGGHNHRFGLYDMI
                     MLKDNHVDFAGGIRNAIVAAQTYCKEKQKDLKIEIEVRNFEELNEVLQVGGVHRIMLD
                     NFSVENTQKAVAIIQGKYEVESSGGITLGTLRQYALCGVDYISVGALTHSVKSLDLSF
                     KAV"
     gene            complement(17487..18305)
                     /gene="panB"
                     /locus_tag="PLJ82_00090"
     CDS             complement(17487..18305)
                     /gene="panB"
                     /locus_tag="PLJ82_00090"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOR39946.1"
                     /translation="MSVHNNDLRKVTTHRLTEMKSRGEKISMLTAYDYSMASIVDQAN
                     MDVILVGDSASNVICGNVTTLPITLNDMIFLATAVTKAVHRALVVVDMPFGSYQGNSK
                     EAVASAIRIMKETGADSLKIEGGQEILESLTRILSTGIPVMGHLGLMPQSINKYGTYS
                     VRAKEEWEANKLMEDAIALEKAGCFAIVLEKIPATLAEKVAKTLRIPVIGIGAGNKVD
                     GQVLVMHDMLGINKGFSPRFLRRYADLHTSISNALQQYISDVKNGDFPSENEQY"
     gene            18455..18778
                     /locus_tag="PLJ82_00095"
     CDS             18455..18778
                     /locus_tag="PLJ82_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR39947.1"
                     /translation="MAEEDLIEFDDEAAISFILNYLPEEVKTKFSDDDVQYLLDVIYE
                     FYDKNGLLHEDVVEEVDIDEEELSAYIVKCAKEDKLPQFTEENVQLLLDAEYEYSKSI
                     GLFKE"
     gene            18794..19696
                     /locus_tag="PLJ82_00100"
     CDS             18794..19696
                     /locus_tag="PLJ82_00100"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR39948.1"
                     /translation="MPQLAYFLLYVVVRLFALLPFFVLYLLSDILYLIVYKLIGYRKK
                     VVRKNLKHSFPSFSQQQLLKIEKEFYHHFCDLFVETIKLSSMSTETIKQRFSVTNMEV
                     VEKLFAQGKSCISLSGHYGNWEWSAAYGLYIKPPAKALFLYKQVKNQAFDKLIQTLRQ
                     RFSGECIEKNFALRKMLQYKKQGVATFTGFLADQTPTARNIHYWTQFMNQDTPVFDGA
                     ERIARSMSYALLYIDVRKIKRGFYELTLIPMSDDVSATTEFEMTEQYVRLLEQTIQRN
                     PSYYLWTHRRWKHKRTQPTTSNIE"
     gene            19697..20734
                     /locus_tag="PLJ82_00105"
     CDS             19697..20734
                     /locus_tag="PLJ82_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR39949.1"
                     /translation="MNKIAIVILNWNGRQYLERFLPSVVMYSTYPHVEIVVIDNASTD
                     TSLEFLSTTYPTITRVVLDKNYGFTGGYNKGVQHIDADYAVFLNSDVEVTANWLDKPI
                     EHLHTHPNSVAVQPKILSFENRAMFEFAGASGGYIDFLGYPFCRGRIISEIEQDNGQY
                     NKPIPIFWASGACLFVRLQEFIQYGGFDDFFFAHMEEIDFCWRMKSKGRDVFCIPESV
                     VYHVGGGTLTKENPHKTYLNFRNNLLLIYKNMLPKQALLVFFIRFFLDYLAAIMMLLQ
                     GKPNDAKAVYRARKDFARHKKAFSAVRLLNMQENVVAQHSEMYTGSILVDFYIRRKRR
                     FSELSIARLFQ"
     gene            complement(20708..22372)
                     /gene="recN"
                     /locus_tag="PLJ82_00110"
     CDS             complement(20708..22372)
                     /gene="recN"
                     /locus_tag="PLJ82_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOR39950.1"
                     /translation="MLLSLHIENYALIKQLDLNFDSGFSVITGETGAGKSIMLGAMGL
                     LLGQRADLKTIKEGENKCIIEASFDIQHYNLKSFFLENDIDYEETTLIRREIVTTGKS
                     RMFVNDTPVTIQFLKEIGQRLIDIHSQHENLLLKNSLFQLDIIDTVATHKDDIVAYTE
                     LYLSYKNKLRTLASLKESAEKEKNNYDYILFQYEQLTSIQLQAGEKDHLEEELHMLSH
                     AEEIKSSLIQANQLLQNDEHGIVTTLKQTQQLIAQLLHFFPQCTDLNERLLSCYIELK
                     DMTAEISKMESSVEYNPQQLQFVTDRLDSIYTLCQKHKVTEIEDLLALQESYKNQLEK
                     ILSFDTEIATLEKECNILYELLIQKATEISINRKTSAPIIEEYMVEQLSTLGMPYVQF
                     KIEFKQASEPQLYGFDDVEFLFSSNKNTIAKPVSDIASGGEIARVMLCLKSLIAQSST
                     LPTIIFDEIDTGVSGEIADKMGNIMQKMSKQMQVICITHLPQIAAKGTQHYQVYKVDT
                     DTSETRVKPLSTTERKEEIAKMLSGSNITEAALKNAEELLKQSSNG"
     gene            complement(22379..23272)
                     /locus_tag="PLJ82_00115"
     CDS             complement(22379..23272)
                     /locus_tag="PLJ82_00115"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOR39951.1"
                     /translation="MRKIQVLFICFIFATITMTAQELVAKVTVNADKIQGVNTQLINN
                     LEKTIAEFFNAQNFTDLVFETNERIDCSFFITIKEINSTNEMKADIQVQARRVAFNSM
                     YYTPLFLFKDENFNFTFTENTILAITDNSFDSNLSAVLTYYAYVIIGLDADSYSKLGG
                     TPFYEKAERITNLAQSTSESGWKAFESSKNRYALITNLLDESLRSFRLFFYDYHRLAL
                     DEMHISVDKARAKIAEDMPTLRAAYKNRPSAIILQVFTEAKVEELLSIFEKGTPNEKK
                     IVYETLSAVSPSLNNKLEPLR"
     gene            complement(23281..24477)
                     /gene="coaBC"
                     /locus_tag="PLJ82_00120"
     CDS             complement(23281..24477)
                     /gene="coaBC"
                     /locus_tag="PLJ82_00120"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOR39952.1"
                     /translation="MLEGKKILLGITGSIAAYKSAYLVRELVKKGAEVKIIMTATAKE
                     FITPLTLATLSKNPILVDFFDPTNGNWNSHVKLGLWADAFLIAPASANTIAKMAHGIA
                     DNLLLTSYLSCKSPVFIAPAMDLDMYAHPSTQENISLLMQRNNYIIEATSGELASGLE
                     GKGRMEEPHIIVNILTNFFSKKSILQGKTILITAGPTYEKIDPVRFIGNYSTGKMGYA
                     LAEECAHNGAKVILISGPVGISVQHPNIMKIHVESAAEMAVATKKYFPVSDAAILSAA
                     VADFTPTIKAAEKIKREKNNLTLELSPTEDIAVAIGKLKKQDQRIIGFALETTNEKQH
                     AFEKLQRKNFDCIVLNSLNDSHAGFGYDTNKISIIDKQGNCEEYDLKLKKEVAKDIVA
                     KLITLF"
     gene            complement(24571..24897)
                     /locus_tag="PLJ82_00125"
     CDS             complement(24571..24897)
                     /locus_tag="PLJ82_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOR39953.1"
                     /translation="MDYKKTNAPKSTISRDTTSLCENTGNIYETVKIIGKRANQISVV
                     IKSDLERKLQEFSSNNVDSLEEIFENREQIEISKYYEQLPKPSLLAIQEYLEDKTYYR
                     NPSKMK"
     gene            complement(24907..25728)
                     /gene="bamD"
                     /locus_tag="PLJ82_00130"
     CDS             complement(24907..25728)
                     /gene="bamD"
                     /locus_tag="PLJ82_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOR39954.1"
                     /translation="MHKFYILFISVMLLLLSSCSNYQKLLKNPDPAIKYEAALEYFAK
                     KDYMRSLTLLQDITPVYRGTEKAENILYLIASSNFHQRDYISARHYYSVYISSYPRGK
                     YIEEVKYMNALSFYKESPEPKLDQTASSKAIELFNDFLLEYPKTDKLVQIQLLVSELQ
                     EKLAERELISATLYYNIGNYRGNNYYRSAIVTAENALKDYPKSKFKEDFSILVVRSKY
                     QEAIQSVENKLYERSVVALDECIYFLQEYPTSNYIKEVQQLKLELEKNNNKIYII"
     gene            complement(25813..26982)
                     /locus_tag="PLJ82_00135"
     CDS             complement(25813..26982)
                     /locus_tag="PLJ82_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR39955.1"
                     /translation="MYSLFNVSFSLFQLIVISCLFIAFVVQLIYYTCCYSKLLTYWNS
                     IKKGKITFNTTLPPVSVIICAKNEADNLQKFLPSILEQDYPSFEVIVVNDGSTDDSED
                     ILMVLKHTYPHLRTTFVPQAAKYIDSKKIALVLGIKAAKNEILLFTDADCHTISKDWI
                     EKIVRNFDDNTEIVLGYGAYRKTKGFVNHLIGYDTLFIAIQYLSFALAKKTYMGVGRN
                     MAYKKSLFYKNKGFASHLNIQSGDDDLFISEASSKTNTKIEIHPNSITVSEPNTSLKR
                     WFNQKQRHISTSKYYKWRSKFILGTEVFSRGLFYISTIALMLALPSHTTYIVVGSFIF
                     VRYIAQITIINYAATILGERKYISSIPLFDILLPLFNLYVWCRSLIHRKQHYKWK"
     gene            complement(26997..28157)
                     /gene="lysA"
                     /locus_tag="PLJ82_00140"
     CDS             complement(26997..28157)
                     /gene="lysA"
                     /locus_tag="PLJ82_00140"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOR39956.1"
                     /translation="MKKGLFPTNKFINLTTPFYYYDTVLLKATLESLKQETNKHNIEA
                     HYAVKANANDRILNIIAKEGFGADCVSGNEIKAALNAGFPASKIVFAGVGKDDWEINY
                     ALENNIFCFNCESIPEIEIINELAHKQQKIASIALRINPNVKVDTHHYITTGVAENKF
                     GINLSDLEKVIDVVKKQANIKLIGLHFHIGSQILEMNNFKSLCTRINEIQQWMLDQKI
                     VIKNINVGGGLGINYQQPNENPIPNFKDYINVITQHLTLQPHQQLHIEPGRSIVAQCG
                     SLIAKVLYIKEGTHTKFAILNAGMTELIRPALYQAYHLIENITSEEEMEKYDIVGPIC
                     ESSDVFAKGMEINRAKRGDLVALRSAGAYGEVMASRYNLRPYPTVYLTEDFQ"
     gene            complement(28180..29499)
                     /locus_tag="PLJ82_00145"
     CDS             complement(28180..29499)
                     /locus_tag="PLJ82_00145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOR39957.1"
                     /translation="MKVLKFGGTSVGSAQRMQHVAKLICDGSQKIVVLSAMSGTTNTL
                     VEISDYLYKKNQGSANEVVNTLEKKYLAIINELYTTTEYKQKATEAIKQIFSYLRSFS
                     KSPVFSLFDEKEILAQGEMMSTNMMHFYLCEQGIKSTLLPALEFMRTDKNNEPDAAFI
                     KEHIAKQLSQYTDTEIFVTQGFICRNAYGEVDNLQRGGSDYSASLIGAAILADEIQIW
                     TDIDGMHNNDPRFVENTKPVAKLHFEEAAELAYFGAKILHPTCILPAKLENIPVRLLN
                     TTDPKAAGTLISTENTKGKIEAVAAKDNITAIKIKSGRMLLAHGFMRKVFEIFESYQT
                     PIDMITTSEVGVSVSIDNTKNLDPIVDELKKFGTVSVDKNMTIICVVGDLPSSNIGFQ
                     AKVVSALENIPIRMISYGGSDHNISFLIDAQDKIKALKALSNKLFNN"
     gene            complement(29699..30973)
                     /locus_tag="PLJ82_00150"
     CDS             complement(29699..30973)
                     /locus_tag="PLJ82_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR39958.1"
                     /translation="MKEITANEFDQEVLKGEKVVVDFYSTECPPCEALFPKYEAVAEL
                     YGHKIKFLKIYRQGNRELATQLGVSSSPTLLFFENGKQILDTISGGIKKSEITARLNK
                     MLTENEIAEIQKKQQRSVSEYDVAILGGGPAGLTAGIYLGQAKVKTVLIDPALPGGYM
                     GITHQVSNYPGFVEAQNGYMLAHYMSEQAKQTGIDFKVAVDITSVDLMKKEIVIDELE
                     TIKAKKIIIATGTSPNTMNAPGEKEYKGKGISYCATCDAKYFVDKEVTVIGGGNSAVE
                     EALFISKFASKITMIHQFDALTANKTAIEQLQANEKITVKYEHEPRKFEKRGEKMVTT
                     IENLRTKEMSELASDGVFVFIGFKANLAFLNTNATPALDNWGYIQTDEDRKTSISDVY
                     AIGDIVSKKYRQITTAVADGTIAAITISKELN"
     gene            complement(30990..32312)
                     /locus_tag="PLJ82_00155"
     CDS             complement(30990..32312)
                     /locus_tag="PLJ82_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR39959.1"
                     /translation="MKKSKHTILVPITDSTDYFVVNTLHKSADILTKNEAKCLKNNID
                     VEGSFTSNKYIVDEETEKRTFKLAYLDFIDKREDEETQLFFVPNYSCNFACSYCYQEG
                     YNPIQQKLTTEIIDAFFAYVATQFAQKKKYITIFGGEPLLPNEQQRELIAYFIKKATT
                     AQLDIAIVTNGYTLLSYIDILKKARIREIQVTLDGTEQVHNNRRYLKGKQSTFNQIVE
                     GIDACIEAEMPINLRMVVDKENIDNLVEFSQFSIDKGWTKSPYFKTQIGRNYELHFCN
                     STPDKLFDRATLYKKIYELLKEHPHIAEFYKPAFSISKYIAENEELPTPLFDSCPACK
                     TEWAFDFTGTIYSCTATVGKKGEELGTFYPHITHNESLIAEWQNRDITTIEACKNCEV
                     ALACGGGCASVAKNKHGHVNSPDCRPIKELLSLGAAYYFSNTPIDTKN"
     gene            complement(32558..33199)
                     /locus_tag="PLJ82_00160"
     CDS             complement(32558..33199)
                     /locus_tag="PLJ82_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOR39960.1"
                     /translation="MITDAINKRYSSLSETTCCLSCGGAINYANPLVGEVCVDLGSGR
                     GNDVVRLAETVGNNGYVYGIDISEGMIAKAQSLIEKFDIKNAKIIQSNLENIPLQDNS
                     VDVVISNCTINHASNKQAVWSEIFRILKPAGRFVVSDIYATHEIAAEYKNDPQAVAEC
                     WAGASTRATYLYTLEETGFESINILEESLPYAKGKAEVASFTVTGKKPIDKIR"
     gene            33306..33764
                     /locus_tag="PLJ82_00165"
     CDS             33306..33764
                     /locus_tag="PLJ82_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR39961.1"
                     /translation="MPEITNEITIMLFKNEISAEFAQQLCEDADKVLEFVSQVKWKEG
                     FVCRNCNHTNYCEGKTPFSRRCTRCKKEESATAHTLFHNIKFPINKAFFIAYHVCMLG
                     DEFSSYTYSFQLGLNQMTCWKFRKRIQHCIAKVDKQDSNTIQGILLQTYE"
     gene            33810..34898
                     /locus_tag="PLJ82_00170"
     CDS             33810..34898
                     /locus_tag="PLJ82_00170"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOR39962.1"
                     /translation="MSYKNLDQQNLSFLKELLSTNSPSGYEVNAAKVWQNYVNQFAEV
                     SGDVLNNSIGVLNPQAEFKIMIAGHYDEIGLQVMYISKEGYLYVRRLGGIDLLSLPGS
                     EVVVITKKGTISGVLGKIPIHVQSPTERTKAIELEDLWIDIGVDSEEEAANLVEVGDP
                     VAIKSNFHYLGQHKIVSKGMDDKIGAFIVAEVLRNLSTQQLKVGVYGVATSQEELGAR
                     GAITSTFKVNPHVGFCLDVGIATDVPGIEERKYGKMRLGGGASITHSADNNVVLVRLL
                     QQTAQQNNIQYQDVASKSATGGTDTAHMQLARQGVATALISVPNRYMHTQVEMCDLRD
                     VDEIISLLVETIKVIDKDAIVDFVPSHM"
     gene            complement(34945..35015)
                     /locus_tag="PLJ82_00175"
     tRNA            complement(34945..35015)
                     /locus_tag="PLJ82_00175"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34981..34983),aa:Cys,seq:gca)
     gene            35185..35886
                     /locus_tag="PLJ82_00180"
     CDS             35185..35886
                     /locus_tag="PLJ82_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR39963.1"
                     /translation="MAKNKKNDATEKELEAIEHVLSSSEAFIEKHQNKLLIGLGVVVV
                     IVLSYLAYSTYYVAPKEKQAAEIVAVGQKYFAQGNYEAVLHGDSVEFDGLIALTDKYS
                     MTTSGNIGAAYVGLAYYKLGEYDNAIKYLNKTSIKDDVLAYTTLGTIGDAYVQKGETQ
                     KGIEYFLKAAKTDNAMVGATYLLKAGKAYESLAKYDKAIEVYQLIKTQYASQLPGFAP
                     IEDIDKYIEKASSLK"
     gene            35889..36380
                     /gene="ribH"
                     /locus_tag="PLJ82_00185"
     CDS             35889..36380
                     /gene="ribH"
                     /locus_tag="PLJ82_00185"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOR39964.1"
                     /translation="MATQYQNLSSYDVASMPLKDIVSVQRYAIVVADWNANITHALLE
                     GAYTSLIENGVLEHNIKVIHVPGAFELTYAAKECQKQKVFAAVITLGCVIQGDTPHFD
                     YVCQGVTQGISILNTKKSVTPVIFGVLTTLTMQQALDRAGGKDGNKGVEAAITAIKMA
                     NIF"
     gene            36421..36503
                     /locus_tag="PLJ82_00190"
     tRNA            36421..36503
                     /locus_tag="PLJ82_00190"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36455..36457,aa:Tyr,seq:gta)
     gene            36511..36583
                     /locus_tag="PLJ82_00195"
     tRNA            36511..36583
                     /locus_tag="PLJ82_00195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36544..36546,aa:Gly,seq:tcc)
     gene            36818..39385
                     /gene="glgP"
                     /locus_tag="PLJ82_00200"
     CDS             36818..39385
                     /gene="glgP"
                     /locus_tag="PLJ82_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOR39965.1"
                     /translation="MKLKVSNVNYPNWRAITVNFKVPKEFEKLQEISKNLWWVWNYEA
                     TDLFKEIDKDLWIEAGGNPVLFLQKLSAEKYKELAKDKAILKKIDKIYADFKAYINVK
                     PSTTIPSVAYFSMEYGLTDILKIYSGGLGVLAGDYLKEASDCNVDMVAVGFMYRYGYF
                     TQALSINGEQVALYEAQQYESLPMEQLLDKNGDPVVISVPYPGRTVYANLYKVAVGRV
                     DLILLDTDTELNNDLDRTITHQLYGGDNEHRLKQEIMLGIGGIAALKAIGLEKDVYHC
                     NEGHAAFLNVQRLIDLMQDKQLSFNEAKEIVRVSCLFTTHTPVPAGHDTFEEPLFERY
                     MFDFATRLGLSWDDFMNMGRETPGDLTEKFSVTVFACNTAQEVNGVSWLHGKVSQDMF
                     KHIWEGYFAEESHVSWVTNGVHYPTWAASEWQKVHEKALGKEYMSDQSNKAIWSKIYD
                     VDDKEIWDTRQALKLKLVEYIKAKFASNWLKNQGDPSRIVSVLERINPNALTIGFGRR
                     FATYKRAHLLFTDLERLSRIVNNPDFPVQFIFTGKAHPADGGGQKLIQRVVEISRRPE
                     FLGKIIFLENYDMAIAKRLISGVDIWLNTPTRPLEASGTSGEKAEMNGVLNFSVLDGW
                     WYEGYKKGAGWALTDKRTYQNQEHQDQLDAATIYSMLENEIVPLYFAKNSKGYSPEWV
                     QYIKNSFALIAPEFTMKRMLDDYIERFYTKLAKRGKSLKAKDFAKAKEIASWKENVAS
                     KWDAIEVVSVELSENLHAQAEVGQSQKATIVVDVKDASLVNSIGVEEVVTHPNKEGVD
                     TFFSAKEVNLVKTEGTLLTFEMNQKVENGGSFKYAFRMFPKNSDLPHRQDFCYVRWF"
     gene            complement(39589..40629)
                     /locus_tag="PLJ82_00205"
     CDS             complement(39589..40629)
                     /locus_tag="PLJ82_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR39966.1"
                     /translation="MKKLILLVASLFFLITQLIGKEKINYELYSVAFYNVENLFDTIP
                     EPNDHEYTPTGAMQWDSKKYWAKMKNMSYAISKIGSNLSPMGPSILGLAEVENRSVLE
                     DLVKQEAIKHINYQIIHIDGGDPRGVDCALLYNPRDFYVTNVKSYPYYTSSDSSRRSR
                     DQLVVSGILSGEEIHVIVNHWPSRRGGEVNSRPRRVAAAQLTKHIADSIFTIDKNAKI
                     IIMGDLNDDPINESVKDVLNAKQKKSETPEGGLYNTTWELYNKGIGSLAYGDAWNLFD
                     QIIVSSGLIGKDKAGLTFWKAEIFQAPFLIRQEGQYKGYPFRTHAGGVYQNGYSDHFP
                     SIIYLIKRIDNK"
     gene            40941..>42058
                     /locus_tag="PLJ82_00210"
     CDS             40941..>42058
                     /locus_tag="PLJ82_00210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2012 domain-containing protein"
                     /protein_id="HOR39967.1"
                     /translation="MKKKILLLLLIANSVLVIAQTGIQGVVRNTTTGDGIADVQVVLK
                     KNNVFVLTNEKGEFTFNELQAGSDVLEIVHPVYVAKPLQVTIAANRISNIGIIRVRDD
                     QSVVREVAQDIANVITLDSELATGDETTGSATRGTALSARGDVYAAATNYTFSAMRFR
                     TRGYDNTYENTYINGVNFNSLPRGMFNYSMLGGLNDAMRNKDVNRGLSPTAYSYGNIA
                     GVTNIDTRASQYALGTRTSVAFSNRSYTLRAQAFHARDLGNDLYLAVSGVARWADEGI
                     IEGSFYNSAGLFTALEKKFADKHSFSLTLFAAPTQRGQQAASLQEVYDLAGSIYYNSY
                     WGYQDGQKRNSRVVKTIDPTAIVSYEWKIDPQQVLRAG"
BASE COUNT        12914 a         6940 c         7772 g        14432 t
ORIGIN      
        1 gtcttcaaca gtttcaaata aggtaaaccc tttgcagctg tttagcagaa tctgtacgtc
       61 atctggtagg gttacatttt tttcttgtaa tcttttaaat aattttgttg tcataatata
      121 tattgtttaa aattctctca gttgcatgag atagactttt gcatatggct acctcacatg
      181 ttaattcgtt tttatgttta tttccgtgac ctgttaatac atatataggt gttaatcccg
      241 ctcgtatacc gcattcacta tcactaggat ggtctccaat tatataagaa tgctttcggt
      301 caatattgta ttcatgtact gcttgattaa taaaataagg acttggtttt ttgcaaaaac
      361 aattgtcttc agtagtgtgc ggacaataat aagttgccgt aattgtaatt ccttgttttt
      421 ttaattctgt ttcaatatat gagtttacta tttttacttc tttttctgtc agtaaattat
      481 ttccaatacc agattgattg gtaataataa acagcataaa gtgttcttgt aaacggatta
      541 aatcagcgaa tgtatttgga aaaaaagaaa ccgcgcttgg ttcttttatg tatcctacat
      601 cttctataag agtgccatct ctgtcaagaa atattgcagg aaatttttgc ttttcttttg
      661 ttatcttctg ttccctatta cacatcacaa agataaaaat ttttttaata caaattttta
      721 ttcatcaata aaaatatcag ataaatgtag ttttacatac ttgctggggc tattactaat
      781 aaaagtttcg tatcctcttg ttgcttattt tgtaatatag agatgttttt cgcgtgaaaa
      841 aatgcactat cacctgtttt caattctact tttttctcat cgatggtaaa ttctatattt
      901 ccttgtaaca cataacacat tcgttgtccg ctgtgtgatg caaatatatt ttcaactttt
      961 tcttgtggtt ttaagattat cacaaaacta tccatttgtt tgttaacatc ttcgtacgga
     1021 atgtgataga ttgtagcctt gtagtcaaga ttatcaagta atttataatc tactgctcta
     1081 tatactggag aatgtttgga tgcctcattc tctccaatta attcaccaac tgtggtatgt
     1141 agattttctg caatcgattt tagtgtaata atagatggaa atgcttttgc attttcgatt
     1201 tgagataatg cactaggagt gataccaact tttgtagcta aatctcctaa tgccatatta
     1261 tagatgcgtc tgtatttttt tattctttct ccgatgcgtt tcattgtgta ctattatttg
     1321 actacaaaga tagtatttta gtttaaataa ttaagtaacg cttaaaatat taatttaaaa
     1381 ttaacatact ttaaataaat ctattgtgtg taaaataaca tgaaacaagc catgagggct
     1441 tctttgtaat cctaccttat ttgtactgtg catatgttta caaaaattga aaacaatagg
     1501 ttatgcaaga ttgttgtacc acacccagta tttgttaggt agtaatatgt ataaaagaat
     1561 ccatatgtgt gctactattg aaaaaactgt tgtctaaatt gcacgggtgt aatgccttcg
     1621 tattttttaa agaacgtaac aaaatgagat gaatcgctaa acgatagtag gtcagctatt
     1681 tcagcaatgg tttttttccg tatgcaggat gtatcgtttg gcttctaaaa gcaggtgatt
     1741 gttaatgtgg tttgtggcat tcgtttttcc atgtttaagg cagatggcat tcaagttttg
     1801 gggtgtcatg tttaataatg cagcatattg gtataccttg tgatgtgtat gtatgtgttg
     1861 ttgaatcaat ttgctaaact gttcgaatgg taaactcatt gattgagctt gtaggtcgtg
     1921 gttttgtgtg gtaattaata aagtggcaaa caacgattgc aataacccat gtatgatgag
     1981 ttgagaaaaa tgatttggtt cgctgtatac cttgtatagc tcttctaata aagcaattgg
     2041 tagtgtttct tttgatatgt tgatgtgtgt atgaaaagcc aattgtttat acaataagca
     2101 tacatttttt tcatgtagag catcaaattc atcattgcta aacaagatga catatccttt
     2161 gggtatggaa gtaaactgcc aataatgcat ttgtccaggt tgcaaaacaa aacaaacagg
     2221 ggcttgaatc aggtaatggg tagattctat ttgatgaaat ccactgcctt cgagcaaaat
     2281 aattagctcg taatagttgt cgtgtttgtg tggtttggtt tttttatttg ctctttaaaa
     2341 cgcgacactt tacatgtagt gttatgcaac ttgcttttaa gttcgatgtt actactgtta
     2401 gtcatcatgg ttatagtaat tgattagttg atgaagtacc gtgcgtaaaa tcgagcatat
     2461 tttgataggt tgtttcggca atttgtgtaa gtgcttcgtg tgtaaagaaa gcttgatgag
     2521 acgtaatcag tacgtttgga aaagtcatta atcgcattaa aatatcatca gaaataatgc
     2581 tttccgaaaa atcgtgaaag aaaagttttt cttcttcctc atatacatca atccccaagt
     2641 aacccaatgc acctgtttta agagcttcta ttgcttcttt ggtatcgata agtgcccctc
     2701 tgctggtgtt tattaacatt gccccgtgct tcattatgct aaattctttg gtactaatca
     2761 tgtgtcgggt atgctgattg agtgggcaat gtaacgaaag tatatcagct tgttttagta
     2821 catcttctaa ggctacatac tggattccta gttgtttggc atcgtcagat tctgttgggt
     2881 cgaatgcgat taccttgcac ccaaaaccaa gcattatttt ggcaaaaacc aatccaatag
     2941 tccctgtgcc aataacacct actgttttgc catgcacatc aaatccgtgt aggcgttcta
     3001 tcgaaaaatt atattctttt acgcggttgt atgctttatg tgtttttctg tttagggtaa
     3061 gaaccagtgc tactgcgtgt tctgctactg catagggaga ataagcgggt acacgatata
     3121 cttttattcc caatgatgtt gcagtgctta tgactacgtt attaaaacct gcacaacgca
     3181 aaaagatggc tttaatacca gcacttgcta tttgttgtaa aaggttgttg tctaatttgt
     3241 cgtttacaaa tacgcaaatg ccatcaaaac cagtaagcaa accaacggtg tttgggttga
     3301 gcgattcttc gaaaaatacg atgctatgtg gtgcaactat agcttttgat aaatattctt
     3361 tgtcgtacga ttttgtacta aagcatgcta ttttcatatg attgacaatt tagagtactc
     3421 ttcaaaacta ctcataaata ctgaaaatag caagtttttt agtctatttc ttgtatcgca
     3481 cgttcaataa tttcgtcttt ggtcaaatct gtccaatcga aacttaccaa aatatctggc
     3541 ggaacaccat cttcggcgta attgttccca tcaaggtcaa gtagttgaga tatgctaaat
     3601 cgataggtcc atccattggg taattgaccg ccatttggca tgcctccacc gccaccagta
     3661 gtatcgccta caagggttaa atttgggatt gccttggttg ataatgcaaa aaatgttgtt
     3721 gcactatacg agccacggtc aataagtacc attatgggtt tagtatatcg tgttccctcg
     3781 tatgggttca tataaaaagt agctctgtcc gaaaaatcat ttcttccttt tccgttacgg
     3841 gtaagtgtat aagcgaccaa ggtgcgtgag tcgataaagc gttctaataa cataggaatg
     3901 ttaaacatgc tacctcctcc attgtcgcgt aagtccaata tgataccatc agtatagcgg
     3961 tatttatcta ataaataatc aagatgagta ttagaaatat catcagagaa acttgcataa
     4021 cgaatgtatg caatattggt acctgagagc attgcatgct taaatgaacc tgttcgttca
     4081 gcatgtggta tgtaaaattc atctattgta cgtgcatgat agttagcagg ttttgtaaga
     4141 ggcacgttat atacagatac attgaatggc gataccaagt tactatggtc atcacgtagt
     4201 tcgttcatta aatcggctag ggtattgaac agctctgttt cgctcatgtt tgggtaaagg
     4261 cgagcttcgt aggttgtttt catttcgtcc caatcgatat tctttagttc aaaatagctg
     4321 tattttttgt ctagttcatt ccatagatat tcaaaattat cgtatgggtt tgaggagctt
     4381 ttatctgcgg aaaaaaagta gttttcgcag gaggtaaggc tgatgcagct tattattaag
     4441 attataattt tgttcatatt gcaattattt atttctaaac attaaagtat attggatttt
     4501 atgggttgcc atttcaaaag gctcgaaacg accaggagct tgtgctatat cccaaatata
     4561 actccatcta tgcccattgc cgtttggtgt aaattgttcc caatataaac gtgttcctaa
     4621 acgatatcca ttaattgtcc atttgtgccc atcaagtaaa aatgccaaag ggttggtatt
     4681 tgtgccatct aacgcaatgt cggcattata tgcataacct ggacggtagt taagattcaa
     4741 taatccaata tttaattgaa acccaatttg tctttttgct ggaaaaagcg tgcgtgtaaa
     4801 aaaatagtaa tcttttatta cgatgttttt tcgagaaaca tcataaatac cttttgttga
     4861 aagcgataaa ttggcaaaac tttccatgcc aaatccattg ttaaataacg aaggattact
     4921 acgtatgttc catgtgttgg ttatagcacc tcctgcttta attgttagtt tatcgtcgta
     4981 attaaaaggt acagtaaaca aagtatgtaa ataaattgaa ccactcataa aggtagaacc
     5041 actatatccc tgtaatatgt tacttaaagg catatatgct gcagtattgg cagatgtaat
     5101 agataattct gtgtaagttt cattttttgg atgtatccat agccagctaa tattgccatt
     5161 tataccagct ccactataaa ataagggaga agtagccaaa tcgcgaaatt tgattaattc
     5221 aataccaaca gatggagtaa aataattttc ccattgtgaa ctctttttta ctacttcttc
     5281 atttgctatt gctaccaatg ctttagatat gataagaaac aatatgatta ttaggcgttt
     5341 tttcatgaaa ttactttttt ctttttttta tatatatacc aatgaatata atacctaaaa
     5401 caacaagtat aatatttccc caaatatatc ccttaccata ttcatttaac aatgaataat
     5461 caaaaatgta ttgataagat tgcattatac ctaaaaacaa aaatacaata ccaataatta
     5521 aaaataaaac tttcatatta ataatttaaa tgtattaagc ttaaaaaaaa tgtaagctaa
     5581 ctgttgaacc atacataata aatatacagc ctgaaaacag gctgtattac aaatcgtatt
     5641 actttctaac tattgtagtt gttggagtat aaagtccact tgttaaagca tttaataatc
     5701 catcaacaaa tgtatgttgt atagttactt cgtagtcagc agcaccacca gccatttcta
     5761 ccgggttcga agtgctaatt ggagccaaac catatagtaa ataatggttc tttttagtta
     5821 tttctacacc agtttgaggt ccgttaccta ctgtataggt gtaggtgtag caagatgtca
     5881 tcactaagca caccgcacag attaaagcta atttaagaat gaaatttttc ataaaaatta
     5941 aaataaaaat aaatatattc ctactcttga ggattttcag ttactccctg ttttgatggt
     6001 ttaacagctc cattattttt ttatatataa gtactaatta gtattacacc taatccaata
     6061 agtgaaaatt tctagtgata tactactttt taaataaaag tgttatatgc aaaacggtat
     6121 tggataatat aatacgttta atccgtttta tataatacag cctgataaaa caggctgtat
     6181 tataaattgc attactttct aacttttgta gttgttggag aatataaacc tccagttaaa
     6241 ccatttaata aaccatcaac aaatgtatgt tggatagtta cttcgtagtt agcagcacca
     6301 ccagccattt ctgttgggtt cgaagtgcta attggcacta aaccagcgat taaataatgg
     6361 ttctttttag taacttctac accagtttga ggtccgttac ctactgtgta agtgtaggtg
     6421 tagcaagatg tcatcaccaa acatactccg cagattaatg ctgctttaag aataaatttt
     6481 ttcataaaaa ataaatttat atttttccta ctcataaggc ttttcggttt cgccccgttt
     6541 ttttaagtgg gttctagact ccactttttc tttttagtta taatataccc gtatttggtc
     6601 ttcttgtaaa cgggttttaa gccaattttt tatggttgat ttattgtcag aacttattgt
     6661 atctccttca agtgaaaata aaataatctg tgtttgttgt gcctcttttt ctttattgat
     6721 atgatgcgag aaaccttgtg taaaggtgca tgcttgaatt tgaggataca tagttctgat
     6781 ttcttttaaa ataattttcc ccatttcatg ttgttgttga atgctatcaa ttttttgttg
     6841 cttaatggct aattgcgtat ccacatggct caactccaat cgtaatttgt atagctccgc
     6901 attgtgttct tctgcaccat caaaggtaaa tccctgacga actaatattt gtgctttatc
     6961 aagattaaaa tccttcgctt tatcaataat tgattggcgt gtttcatcat ccatggaact
     7021 gcctccatat accagagaaa tagactcgtt tttagtatct atatcgctct ttagtaggta
     7081 tatatcgtcg agcaaactta cgttagagat gtatttagtt gcattttcgg taaatttttc
     7141 tttcaccacc aaggtgtaac caaaataaat actaggtaaa aatacaatag caataactgt
     7201 agaaatatac cggtttacac gtcgtttttt tctttcatca actaatgtgc gtattggaaa
     7261 ttttagcaac tgagagacaa ataaggaagc taaggctata aatacagtat tgatagtaaa
     7321 taagtagatg gcaccaaaaa agaaattcat ctgaaatgta gctaatccgt acccagctgt
     7381 acacaacgga ggcataaggg ctgttgcaat ggctacgcct ggtataacat ttcctttttg
     7441 tttgctacta atggcaataa taccagctaa gccaccaaat aaggcaatca gtacatcgta
     7501 tatagatggg cttgtgcggg caagtaattc agaatgagct gttgatatgg ggcttaaagt
     7561 gaagtatatc gtagaggcaa ccaaactggc aattacggca aaagaaaagt tttttactgc
     7621 acgtttaaat aaggcaatat cgtacgtggc aatgctatac cccatgccat tgattggacc
     7681 cataagcgga gagataagca tagcaccaat caccacggct gttgaattca tatttaaacc
     7741 aacagaggct acaatgatag caaacatcaa aatccataaa ttagtccctt taaaagcaat
     7801 atccttaaca atactagaat ggatagcatc gaattcatct agttcgggga tgatagtaac
     7861 atgtgataca agttttttta gcaatatgtt catttctatg taggttatat tttttttcta
     7921 aatatttttg ctattgtttt tccaatttca tccaattttt caataggttc taaaatggta
     7981 gggttagaaa tgtccatcat agacgtttct cctacgccca cttgcattgg ataaaccagt
     8041 atataactat ttccctcaaa atatttattg agatatgagg gtatacgttg catagatggg
     8101 gtataagagg gtttgtcttt gcgactcaga ataacaatta agttgtcatt tgcctcaata
     8161 tctcttgata atattaaaaa atcatcccaa tctgaaaact ctttaaaggt gcattcgatt
     8221 ggatgtttgg attgaatttc tcgaattact ttttgggtat ctatacttga gtaaaagaca
     8281 atctttgctc ccgaattgcg tgcgatattc catgttttga taatccaaaa aggaaatcct
     8341 atttcttttt cagctttatc aggtacaatt actaaatggc gtttgatagt gccaaaaggt
     8401 tgtgttggtt tataaattaa ggtagtaggg ttcgacttgc tcaatatgcc taaagtaagg
     8461 ttgctaaaaa atccatctga aatccctttc tcattgttta agcctaaaat taaatcggtg
     8521 atgcgttgct cttttaggct acttgtgata ccatttacta aattaatatc gtagcgcaat
     8581 agttcattta atttagtatc agttgcagca gcaatacgtc ctgcctgttg taacagcttt
     8641 ttcgcttgtt tttcgttatt gttctgtgat tcgtcgttgt caataatatg tagagcatac
     8701 agcccttttt tgttgtgttt tgacttaatt gttaccccta aatttaccaa ttcttctaaa
     8761 ttgttttctt ggttaatggc aattagaatg cgttcgtctg tttcaacttc acccgttgaa
     8821 gattttaaat cttcggataa agcaatattt tttgctcctt tttgagcttg aaaagatgca
     8881 attgtacagg ttactaatat catcaaaata gttccgttta gcacactagc atcgaaaatt
     8941 tgaagattgt atccaattaa taccgcggct aaggtggctg ctgcttgtgc attgcttaat
     9001 ccaaaaataa ttcttcgttc atcaagacta agtttgtatg ttttttgagt aagccaagct
     9061 gctaaaaatt ttgctaatgt agcgataata gtcattactg ctgctacttt taaggtttcc
     9121 caatctttaa aaaatgctcg gtaatcaacc aacattccta caccgattaa gaagaaagga
     9181 ataaaaatgg catttcccac aaattccact ctattcatga gcggagaagt atgtggtatg
     9241 agtctattta aagctaaccc cgttaagaat gccccaataa tagcctctat accagctaat
     9301 tcagctaaag tagctcctaa aaagaccatt gctaacacaa aaatatattg ggctacatta
     9361 tcatcaaatt tcttaaaaaa ccatcgtgca attattggaa ataggatagt tataattaat
     9421 ccaaatgcta aaagggaaac tgataagcga atccaaaaat cgctattaac ctcgccattt
     9481 gccatcccaa ctattactgc caataccaat aaagcaagtg tgtctgttat catcgtgcct
     9541 cctactgcca ttgttacagc tttatttttt actacaccca atttgctaat taaggggtag
     9601 gcaataaggg tatgggaagc aaacatactg gcaagaagta ccgatgtggt aatagaaaat
     9661 tgaagaatga atatactgcc caatatgcct aataccatag gaatgataaa cgtgtacatc
     9721 ccgaaaataa gactacgact tgtgtttttt ttaaaatcag caatatctat ttctaagcct
     9781 gctaaaaaca tgatatatag caaaccggct gtccctgaaa gaatgattcc actatcacgt
     9841 tcgattaaat ttaacccaaa aggaccaatg atggctcctg caataattaa tccaagcaaa
     9901 tgagggattt ttattttatt taagaatatg ggtgcggcta agatgatgat aagaattaac
     9961 aaaaatttca acacaggatt ggttaatggt agtgttgttt caaatatcgt ggctaaaatc
    10021 ataggtggtg tgttgatttg tggcaaagat agaaaaaaat attctacttt atatagcttt
    10081 tttttacctt tttaagcctt ttttatgaga atttaccctg atatagcttt tttatacgtt
    10141 tcgatagccc gattacgtgc aaatgtatgc tcaacgatgg gtgtagcgta cttgtttgta
    10201 tctacttcag gtatccattg acgaatgtat tcgctgtttg aatcaaactt ttttgcttgc
    10261 tctgtagggt taaaaaccct aaaatagggt gccgcatcgc agccacaacc tgcagcccat
    10321 tgccagttgc cattattggc agataaatcg taatccatca atttggtggc aaaatacgct
    10381 tcgccccaac gccaatctac caataagtgt ttgcacaaaa aactagctac taccattctt
    10441 actctgttgt gcatccaacc cgtttcgttt agttgacgca ttcctgcatc taccaatgga
    10501 tatccagtag tcccattgca ccatcgactg aattctgcgg tatcgtttct ccattcaata
    10561 gcatcgtatt ttggtttaaa agcatttgtt tgtacgtgtg ggaaaaaata caagatttgc
    10621 ataaaaaatt ctcgccaaat cagttcgctt acatatgtat gattacgtgt aaaagctatg
    10681 cgtgccaatt gtcggatgct tatggttcca aagcgtagat ggatgcttag tgttgaagtg
    10741 ccatttatgg ctggaaaatt tcgtgtatct gtatagttgt caataattga gtaggggata
    10801 ctggggggtg tgtactgtat gctcgttttt ttaaacccac attttgcgag tgatggtatt
    10861 tcttgagcag gtgttgacaa ccaatttttc ttatatgcgt cagtggtgta ttcttgatga
    10921 ggtagttgct cgtatttacg tagccatatt tttgaaaaag gcgtaaaaat agtataagga
    10981 cttccgtctg cttttattac ttcgcctggt gcaaaaataa cttgatcagt ataggtatgt
    11041 aaggtgattc cttttgtatg caacaacgaa cgtacttctt tgtcgcgttg aatggcgtaa
    11101 ggctcataat cagagtttgt atgtactgct ttaatcgtaa attgagtggt caatttttcg
    11161 aaaacctcgg ttggtttgcc aatttctacc atcaacgatg agcctaattg atgcaattct
    11221 tggttaagag atgtaagaat ttgatgaata taatctaccc gcttatcata cgtagaaggc
    11281 aaggaagata aaatgtctgt atcgaaaata aatagaggga atacaggata tcgtttgtct
    11341 tgcaaggcat tgtatagacc aacattgtca gtcaaacgta ggtcgcgacg aaaccaaaaa
    11401 acctgtatgt cttgtccctc tttttgcatg cttattttct aaacgtagta tcgtaatata
    11461 caaaactctc ggcaacaatc tccaaagcag cttttttgtt caaaaattca tctacctcta
    11521 cgtgtttctt ttccagtttt ttatagtctt cgaaaaagtt tcttaattcc gaaataaaat
    11581 gaggtggcag ttcctctata tcgttgtaat gactcacact tgggtcgcca gttgctactg
    11641 ctattatttt attgtcagtt gcaccgctat ctaccatttg cattactcca ataactttgg
    11701 cagttactat acacatgggg gtaagttcaa tcattgatag caccaaaata tccaatgggt
    11761 ctttgtcgtt gcaataactt tgcggaataa atccgtagtt tacaggataa tacactgatg
    11821 agtacaacac tcgatcgagt ttaattaagc cactttcttt gtcgagttcg tattttgccc
    11881 tacttccttt gggtatttcg ataattgctt gcacactatc cgtttgcgta gtatgaggcg
    11941 atacgtggtg ccaaggattc atgtaattag tcatatagat acttttttta ttgatttaat
    12001 aattttcata agcagaaagg aagtagcgta aatataccat ctttggacct actacacgca
    12061 tgcgttcttc catcattttg cgtttattgg gggcataaca atgagttggg catttgcggc
    12121 aactaggttt gttttcgccg tatttgcagg cacttaaacg agctaacgca taagctgtta
    12181 atgttttgca ttcctcgcaa agttgccctt gttggtgctt ctttttgcaa taataagcta
    12241 tcatgccttt tatcgttcgc tgttgttgtt caatatagga tatttttttt gttcctgtca
    12301 ttatcgtaac aatacaaatg gagttagcat tttacaattt catcgctaag ataagtataa
    12361 ttatacaaaa tggcactatg aaaatgtact taattccgtg tgtgtagggc aaattttttc
    12421 tggaatcata aaagaagtaa aaaaaactgg gataaacagg tataggtatt cttttttatt
    12481 atttttgtag tttaaattcc attcttctga tttctataat ttttttaatg ttgctatact
    12541 atgaataaaa ttgtaaaaag aatacttgtc agttttttag tgttgtttat tcttgttttt
    12601 gccaccttgc ttacggctcc ttactttttc aaagataaga ttttgacctt agcaaaacaa
    12661 gaagtcaatg cactgttgaa cgctcgagcc gatttttcgg gtataaaatt gagttttatt
    12721 cgcaattttc caaatgcaac cgttcagttg caagatttta gtatcgttgg tgtagccgat
    12781 tttgaagcag ataccttgtt ttatgccaaa gattttggca tggttgtaaa cctgaaaagt
    12841 ttatttgccg ataatggtta cgatgtaaaa cgcatagaag ttgacgaagc atccgttaag
    12901 ttgcgtattt tgcctgatgg gagagccaat tgggatataa tgaaagaaga taccttggca
    12961 gtagaagata cctcagcatt tgcttttcat tttaagttgc aagattttac aattaaacgc
    13021 accaattttt cctatattga cgaagaaggc gatatgcaat tgtatcttac taatatcaac
    13081 caccatacta cgggcgattt aactgccgat agctcacttc ttgtaaccaa aacaacagcc
    13141 gaaacggtaa cattcgtgat ggagggtatg gagtatctat ccaaagctac tgccgttttc
    13201 gatgctacta tcaatgccaa cttgaatgat atgaagttta catttagcga aaatcagtcg
    13261 caattaaatg ctgttccctt tgcctttgac ggttggctca aaagcattga taatgggtgg
    13321 gctatggata ttacgttgtt ggcaagtgag gtcgatttta agtctttgtt atcgcttgta
    13381 cctgctgtat atgccactga ttttgccagc ttgcaagctg acggcaaggt gtcgttaact
    13441 ggttttgtaa aaggcgatat ggtaggtgat tattatcctg catttggttt gcagttagcg
    13501 gtagaaaatg cgtggtttaa gtatcctgat ttaccaaaaa aattagatca aataaccatt
    13561 aaaacagcca ttaccaatcc tggaaaaacc ttggatgaaa cagtaattga tgtgtcacgt
    13621 tttgctttcc gtatgggaaa caatcctttt gctgcctcta tgcatattac tacacctatg
    13681 tcggacccaa atgtggattt taaagccaat ggcaaactca atttatccga catacaagag
    13741 ttctacccat tagaacaagg cattacctta caaggattat tggatgtaga tatgttgcta
    13801 gcgggcagaa tgtcgtatta cgaaaaaaat cagtacgata aatttacgtt tgccggaaaa
    13861 atcgatatta cgaagatgca tcttgtatac ccgtcgttac cacaagcact gaaaataaac
    13921 aaggctagtg ctctcatcaa taatcgtttt gtggagcttt ctacattaga tatgcaaatt
    13981 ggcagaaacg acatacaagc acaagggaaa ttggagaatg tagtgccgta tgtgcttaaa
    14041 aatcaaactt tagacggaca attagccttg caatcaagat attttaacgc tagcgatttt
    14101 atgccacaag atagtgtgca agaagaaacg gatgaggagc caatgtccgt aatcgtattg
    14161 cccaaaaata tccattttgt atgtactgcc acatttgatg agttggtgta cgaaaaaatg
    14221 aattttacta acgcaaatgg aaaattgacc attgccgatg gagatttaac catcaataat
    14281 atgtctgtaa atgcgtttgg aggtaaaatg ctaatgaacg gtgtgtatag tacgaccgaa
    14341 cccgaaaaac cacgtgttga ttttaccttg caaattcaag atgtattgtt cagcgaggtt
    14401 tcaaaacaag tagaaacgct gagtaagttt gctcctattt tgggcaatgc cttgggtaaa
    14461 ttctctacta ctttttcgtt taattcgttg ttgcaacaag atatgatgcc cgatttagcc
    14521 agttttatcg gcagtggaaa actcaccacc tcatcagtag gattagaaaa tgtacctgcc
    14581 ttaaatgcca tagccaaatc gctaaaacgt gatgatattg tgccaatggt agtaaaagat
    14641 attgccatga tgtttgaaat taataatggg aaagtaacaa cgaaaccttt taagtttaaa
    14701 gtaaaagatg ttgattttac ccttggtgga caaaccggat tagacaaatc cattgcttac
    14761 acaggtaatg tgcgtttgcc cgacaaactt aatcttggca aattttctaa catggcaata
    14821 gccgttgggg gcacatttac caagccaaca gtaaaagtag acttacttag cactttggga
    14881 gctgtggtag aagagaaaaa acaagaaata aaagcacaag taaccgaaaa agtggacgat
    14941 gccaaagaaa aagccttaga agatgcacgt ctgcgtagag ccaaagcctt ggaacaagcc
    15001 aataaagaag cggataaatt agtgcaagaa gccaaagttg ctggagagcg tttggtagcc
    15061 gaagccgaca aaaaaggcaa agcattggtt gctcaggcaa acaatccatt aactaaacga
    15121 gctgctgaag tatccgctcg cgaaatggta aaagaagctc aaaaacaggc agataacctg
    15181 aacaaacgtg cagaggaaga agccaaacgt atcttgctca aagccgaaca aagtagcacg
    15241 ttttaagcgg gtttttgtgc gataggcttg tggttttctg agctaagagc taacgtacat
    15301 actccgagta actctgtgta ctattcttac acgaagagca cgaagtaaac acgaagatac
    15361 attgagaaaa atatatacaa ctccgtgcca ctctgtgaaa cctccgtgtc tctctgtgta
    15421 ataaatctca cacgaagagc acaaagatag ctatttcgct ccgctggagc taacaaacaa
    15481 atcggtatgt attttctatt atgatgtagc ccctacgggg catcaaatac ttcgtgccac
    15541 tctgtgaaac ctccgtgtct ctctgtgtac tatttcttac acgaaaaaca aacgacctcc
    15601 gtataataaa tcccccaaaa accaatcact atccactatc cactgtcaac tatccactaa
    15661 ttattccctc attcatgatg ataaggttca ttattgagaa tagtaaaagc cctgtatagt
    15721 tgttccacaa aaataaggcg aatcatttga tgcgaaaaag tcatttttga tagcgatatt
    15781 ttttgcgtag ctttttgata tatttgttcg gaaaatccat acggaccacc aatcacaaat
    15841 agtatttttt tctttccgtt tacttgagcc ttgtcaatgt gctgagcaaa atctacggag
    15901 ctaaactgag ttcctttttc atctaacagg ataatatcgt ttgcatcttc cagttgtttt
    15961 aggatacatt caccttcttt ctctttttgc atttctatag atagatgctt cgcattttta
    16021 agttctggaa tcacctgtaa atcaaatgat acatagtgtt tcaggcgatt ttggtaatca
    16081 gttatcaaat tctgtaaata agtatttgtc gtttttccga tcacaagtag tacaattctc
    16141 attttttttg attatgtttg cattgtattc tgcaaaagta gaacaaaact gcataacttc
    16201 ccaatatgaa gaaaactaaa tacatatatg tgttattgcc actgatgttt tttttcattg
    16261 ggggaggtgt ttcggtggca caacaagaaa tgattgaccg tatttcggag ggttttgtac
    16321 gtggaaatgt ttcgtttgtt tctccttatt ttaatgatag aatggaattg tcaatattgg
    16381 gacaacatat tggagtgtat acaaaattgc aatcccaaga aattgtacaa gcttttttga
    16441 cgaataatac accacaatcg tttaatatat cgcacaaagg cataaaagaa aattcaggct
    16501 ttgctattgg tatccttaca actacaaaca acaaaaagta tagggtgtat attttggtgc
    16561 gaaatacgaa tgaaaaatcc cttattcatc agctaagaat tgacgaaaca aatgattaat
    16621 gatattgctt atttactaaa agagtggttt tcggaggata taggcgacgg agaccattcg
    16681 tctttttcat gtatcccttc caatgccatg ggcacttcaa gattattagt taaagaggat
    16741 ggcgttattg ctggagtgga ggttgctaag caggtgtttg cccattttga taacacattg
    16801 caagtaacag tttttatccc cgatggcaca aaagtaaaaa aaggagatat cgtgtttgac
    16861 gtaacgggta atgtacgctc tttgttgcaa acagaacgat tgatgttgaa tctaatgcaa
    16921 cgtatgagtg gtattgcctc cgttacacgg gtgtatgtag atgaattgca aggtcttgct
    16981 acaaaagtgc ttgatacacg caaaacaaca ccaggtctac gttttctaga aaaaatggct
    17041 gtaaaaattg gtggcggtca taaccatcgt tttggattgt atgatatgat tatgttgaaa
    17101 gataatcatg tggattttgc aggtggcatt cgtaatgcaa ttgttgctgc ccaaacctat
    17161 tgcaaagaaa agcagaaaga cttgaaaata gagattgaag ttagaaattt tgaagagtta
    17221 aacgaagtgc ttcaagtggg tggtgtacat cgtattatgt tggataattt ttctgttgaa
    17281 aatacgcaaa aagccgttgc cattattcaa gggaagtacg aggtagagtc ctcgggtggc
    17341 ataacactag gcaccttacg ccagtatgcg ttgtgtgggg tagattatat atctgttgga
    17401 gcattgacgc attctgtaaa atcgcttgat ttaagtttta aagcagtata aaagaaaaag
    17461 aagagaaatt tctcttcttt ttttcgttaa tattgttcgt tttcgctcgg aaaatcaccg
    17521 ttcttaacat cacttatata ttgttgcaat gcattgctga tggaagtgtg caaatcagcg
    17581 tacctgcgta aaaagcgagg agaaaatcct ttgttaatac ccaacatatc gtgcattacc
    17641 aacacttgtc cgtctacttt attgcctgct ccgataccaa ttacgggtat gcgaagcgtt
    17701 ttagctactt tttcggctag tgtggcagga attttttcca acacaatggc aaaacaaccc
    17761 gctttttcaa gggcaatggc gtcttccatg agcttattgg cttcccattc ttctttggca
    17821 cgtaccgaat atgtgccgta tttatttatt gattgaggca ttaaacccaa gtgacccata
    17881 acgggtatac cagtggataa aatgcgagtt aatgattcaa gaatctcttg accaccttca
    17941 atttttaagc tatctgctcc agtctctttc ataatacgga tagcggaagc aacagcctct
    18001 ttagaattgc cttggtagga accaaatggc atatccacaa caaccaaagc tctatgtaca
    18061 gcctttgtaa cagcagttgc taaaaaaatc atatcattaa gtgtaatggg cagggtagtt
    18121 acattgccac aaataacatt agatgctgag tcgccaacaa gaataacatc catattagcc
    18181 tgatcgacaa tactcgccat cgaataatcg tatgctgtaa gcatggaaat cttttctcca
    18241 cgcgatttca tttcggtcaa tcgatgtgtc gttacttttc tcaaatcatt attatgtact
    18301 gacatagttg ttctttttaa ttaaacaaga ttgcaaattt acgaataaaa tcttattcaa
    18361 attgccgata ttaaaaaata attaaaattc attgctaata acaatttcat ttgtatcttt
    18421 acctaaaatt atttaaccag ttaaatttct agttatggca gaagaggatt taatagaatt
    18481 tgacgacgaa gcagctatct cgtttattct taattattta cccgaagagg tgaaaacaaa
    18541 gttttcggat gatgatgtac aatatttatt ggatgttatc tatgaatttt acgataaaaa
    18601 tggactcttg cacgaagacg tggtagagga agtggatatc gacgaagaag aattgtcggc
    18661 gtatattgtt aaatgtgcca aagaggataa attgccacaa tttacagaag aaaatgttca
    18721 gttgctatta gatgctgaat acgagtattc taaatcaatc ggtttgttta aagaataaga
    18781 tacatttttg ctaatgccac aattagctta ttttttgttg tatgttgtag taaggctttt
    18841 tgctttactg ccattttttg tgttgtatct actgtcagat atactttatt tgattgtata
    18901 taagctaatt ggctatagaa aaaaagtagt acgcaagaat cttaaacatt cttttccttc
    18961 tttttcgcaa caacaattac tgaagataga gaaagagttt tatcatcatt tttgtgattt
    19021 gtttgtagaa actatcaaac tgagtagtat gtccactgaa actatcaagc aacgttttag
    19081 tgtaacgaat atggaggtag ttgaaaaact ttttgctcaa ggtaaaagtt gtatatcgtt
    19141 gtcaggacat tatggtaatt gggagtggtc ggcagcttat ggtttatata taaaacctcc
    19201 agctaaagcg ttgtttttgt ataagcaagt taaaaatcaa gcatttgata aattaataca
    19261 aacattacgt cagcgttttt ctggcgagtg tattgaaaag aattttgcat tacgtaagat
    19321 gttgcagtat aagaaacaag gtgttgctac atttactggt tttctggcag accaaacacc
    19381 tactgcacgt aatattcatt attggacaca atttatgaat caagatacac ccgtgtttga
    19441 tggagctgaa cgtatagcac gtagtatgtc atatgcttta ttgtatattg atgtccgaaa
    19501 aataaaaaga gggttttatg aactaactct tattccgatg tctgatgatg tttctgctac
    19561 aactgaattt gaaatgacag agcaatatgt tcgtttactt gagcagacta ttcaacgtaa
    19621 tccatcttat tatttgtgga cacaccgacg ttggaaacat aaacgaactc aaccaacaac
    19681 tagtaatata gaataaatga ataaaattgc catagttata ttaaattgga atgggagaca
    19741 atatttagaa cgattcttgc ctagtgttgt gatgtactct acctatccac atgtagaaat
    19801 agtagtgatt gataatgcat ctactgatac atcgctagag tttctttcaa ctacctatcc
    19861 aacaataact cgtgtagtat tggataaaaa ttatggtttt actgggggat ataataaggg
    19921 tgttcaacat attgatgccg attatgcggt ttttctcaat tcagatgtag aagtaacagc
    19981 taattggtta gataaaccta tagagcattt acatacccat cctaatagtg ttgccgttca
    20041 acctaaaatt ttgagttttg aaaatagagc tatgtttgag tttgcaggag cgagtggagg
    20101 ctatattgat tttttaggtt atcccttttg tagaggaaga attattagtg aaatagagca
    20161 agataacgga cagtataata agcctatccc cattttttgg gcttcaggag cttgtctgtt
    20221 tgtgcgtttg caggagttta ttcaatatgg tggttttgat gattttttct ttgcacacat
    20281 ggaagaaata gatttttgtt ggcgtatgaa atcaaaaggg agggatgttt tttgtattcc
    20341 agagagcgta gtttatcatg taggaggtgg aaccttaacc aaagagaatc ctcataaaac
    20401 atatttaaat ttcagaaata atttattgtt aatctataaa aatatgttgc ctaaacaggc
    20461 tctattggtg ttttttatac gtttcttttt agattattta gcagccatta tgatgctctt
    20521 acaaggaaaa ccgaatgatg caaaggcagt atatagggcg agaaaagatt ttgctaggca
    20581 taaaaaagct tttagtgcag ttcggttact caatatgcaa gaaaatgtcg ttgctcagca
    20641 ttcagaaatg tatacaggaa gtattcttgt cgatttttat attcgacgta aaagaagatt
    20701 ttctgaatta tccattgctc gattgtttca gtaattcttc tgcatttttt aacgcagctt
    20761 ctgtaatatt actaccacta agcattttag caatttcttc ttttctttct gttgtagaaa
    20821 gtggtttcac tcttgtttct gaggtgtctg tatctacttt atatacttga tagtgttgtg
    20881 ttccttttgc tgcaatttgt ggtaagtggg tgatacaaat aacttgcatt tgtttactca
    20941 ttttttgcat tatgttcccc attttatctg caatttcacc cgatacgcca gtatcaattt
    21001 catcaaaaat aatagttggt aaagttgagc tttgtgctat aaggcttttc aaacataaca
    21061 ttacacgagc aatttcccct ccagaagcaa tgtcgcttac aggctttgct atcgtgtttt
    21121 tattggatga aaataaaaat tctacatcgt caaaaccata taattgaggt tctgatgctt
    21181 gtttaaactc aattttaaat tgcacatacg gcatccctaa ggtagatagt tgttctacca
    21241 tgtattcttc aattattggt gctgaagttt ttctgttaat agatatttca gtcgcttttt
    21301 gtatgagtaa ttcgtataat atattgcatt ctttttctaa tgtggctatt tctgtatcaa
    21361 aggacagtat tttttcaagt tgatttttgt atgattcttg taatgctagc agatcttcaa
    21421 tttcggttac cttatgtttt tgacacaagg tatagatact gtcaagtcta tcagttacaa
    21481 attgaagttg ttggggattg tattctactg agctttccat tttgcttatt tcagcagtca
    21541 tatcttttaa ttcaatataa cacgataaaa gtctttcatt caaatccgta cattgcggga
    21601 aaaaatgtag tagctgtgca ataagctgtt gcgtttgttt aagtgtagtt actatgccat
    21661 gttcatcgtt ttgcagtaat tgattagcct gtatcaaaga ggatttaatt tcttcggcat
    21721 gcgatagcat gtgcaattct tcttctaaat ggtctttttc gccagcctgt agttgtatac
    21781 ttgttaattg ttcgtattga aataaaatgt aatcgtaatt gtttttttct ttttctgcag
    21841 attcttttaa tgaagctaga gtccgaagtt tatttttata cgaaagatat aattctgtat
    21901 atgcaactat gtcatcttta tgtgtagcca cagtatcaat gatatccaat tgaaaaagag
    21961 aattcttaag taagaggttt tcatgttgcg agtgtatatc aataagtcgc tgtccaattt
    22021 cttttaaaaa ttgaatagtt acaggagtat cattgacaaa catgcgtgat tttcctgttg
    22081 ttacgatttc ccttcgtata agggttgttt cttcataatc tatatcattt tcaaggaaga
    22141 aggactttaa attatagtgt tgaatatcaa aactggcttc aataatacat ttgttttctc
    22201 cttctttaat tgtttttaag tcagctcgtt gacccaacaa caatcccatt gctcctaaca
    22261 taatagattt ccctgctcct gtttcacctg taataacaga aaaacctgag tcaaagttta
    22321 agtctaattg tttgattaga gcataatttt ctatgtgtaa agataataac ataggatttt
    22381 atcgtagcgg ttcaagttta ttattgagtg atggagaaac agctgaaagt gtttcataaa
    22441 ctattttctt ttcattgggc gtcccttttt caaagataga cagtaattct tcaacttttg
    22501 cttccgtaaa tacttgtaaa ataatggctg acggtctatt tttatatgca gctcgtaaag
    22561 tgggcatatc ttctgcaatt ttagctctag ctttatccac cgaaatatgc atttcgtcga
    22621 gagctaaacg atgatagtca tagaaaaata agcgaaaaga acgtaaactt tcgtcgagta
    22681 ggtttgtgat tagggcatac ctattttttg agctttcaaa ggctttccat ccgctttcag
    22741 aagtgctttg agcaagattt gttattcgtt ctgctttttc gtaaaaaggt gttccgccta
    22801 gtttgctata agaatctgca tctaatccaa tgataacata ggcatagtaa gttaacacag
    22861 cagataggtt cgaatcaaaa gagttgtctg taatagcaag aatagtgttt tctgtaaaag
    22921 tgaaattaaa attttcatct ttaaaaagga aaagaggggt ataatacata ctattgaatg
    22981 caactcttcg agcttgcact tgtatgtcgg ctttcatttc gttagtgcta tttatttctt
    23041 tgattgttat aaaaaaagag cagtcaatac gctcatttgt ttcaaatact aaatcagtga
    23101 aattttgtgc attgaaaaat tcagcaatag ttttttcgag gttattaata agttgagtat
    23161 ttaccccctg tattttatct gcattaacag taacttttgc tacaagttct tgagcagtca
    23221 tcgtgatagt agcaaaaata aaacaaataa aaagcacctg tatttttctc atgtcgcttg
    23281 tcaaaaaagg gtaattaatt tggctacaat atcttttgca acttcttttt ttaattttag
    23341 gtcatattct tcgcagtttc cttgtttgtc aatgatgcta attttgtttg tgtcgtagcc
    23401 aaagcctgca tgagaatcat ttaatgaatt gagcacaata caatcaaaat ttttgcgttg
    23461 tagtttttca aatgcatgct gtttttcatt tgtagtttct aatgcaaacc ctataatccg
    23521 ttggtcttgc ttttttaatt ttcctatcgc tactgctata tcctcagtag gagaaagttc
    23581 tagcgtaaga ttattttttt ctcgttttat tttttcagca gcttttattg ttggtgtgaa
    23641 atcagcaacg gcagcgctca aaattgctgc atcagaaact gggaaatatt tttttgtggc
    23701 tactgccatt tctgctgccg attcaacatg tattttcata atgtttggat gctgtacact
    23761 tattcccact ggtccagaaa ttagaatgac cttagctccg ttatgggcac attcttcagc
    23821 cagtgcataa cccatttttc ctgtagaata attcccaata aaccgtactg gatcaatttt
    23881 ttcataggta ggaccagcag taataaggat ggttttacct tgtaaaatag atttttttga
    23941 aaaaaagtta gttagtatat tcacaataat atgaggttct tccattcttc cttttccttc
    24001 caatccactt gctaattctc cactggtggc ttcaataata tagttattgc gttgcatcaa
    24061 cagagagatg ttttcttgtg tactaggatg tgcatacatg tctaagtcca tagctggagc
    24121 tataaacact gggcttttgc acgataaata ggatgttagt agtagattgt cagctattcc
    24181 atgtgccatt tttgcaatag tatttgctga agcgggagca attaaaaaag catctgccca
    24241 taagcctaat ttcacatgac tattccagtt tccatttgta ggatcaaaga agtcaactaa
    24301 aataggattt tttgataaag ttgctaaggt aagtggtgtg atgaattctt tggcagtagc
    24361 cgtcatgata attttaactt cggctccttt ttttaccaat tcgcgtacca agtatgctga
    24421 tttatatgca gctatactcc ccgttattcc caataaaatt ttctttcctt ctaacatagg
    24481 tttttataaa aaagataaaa gaatatcgaa acggaaaaca accaccccca cattctttta
    24541 tcaaaaatta gtactataac aattcgtagg ttatttcatt ttcgacggat tgcgatagta
    24601 tgttttatct tctaaatatt cttgtatcgc aagcaaagaa ggttttggta attgttcgta
    24661 atattttgaa atttcaattt gttctctatt ttcaaagatt tcttctaagc tatctacgtt
    24721 atttgaagaa aattcttgta attttcgttc caaatcactt ttaatcacaa cgcttatttg
    24781 atttgctctt tttccaataa ttttaactgt ttcataaatg tttccagtgt tttcacacaa
    24841 gcttgtggtg tctctagaaa tcgtactttt gggggcattc gtttttttgt agtccatacg
    24901 ttttagctaa attatgtaaa ttttattatt atttttttca agttccaact taagttgctg
    24961 aacttctttt atataattac ttgtgggata ttcttgtaaa aaataaatgc attcatctaa
    25021 agccacaaca cttcgttcgt aaagcttatt ttctacactt tgaatggctt cttggtattt
    25081 tgaccgtaca actaaaatag aaaaatcttc cttaaatttt gattttgggt aatctttcaa
    25141 tgcattttct gcggtaacta tggctgaacg atagtagtta ttgccacgat aattacctat
    25201 attataatac aaggtggcac taataagttc tctttcagct aatttttctt gaagttcact
    25261 aactaaaagt tgaatctgta ctaacttatc cgttttcggg tattctagta aaaaatcgtt
    25321 gaacaattca atggctttac tactcgcagt ttgatctaat ttaggttctg gagattcttt
    25381 atagaaactc aatgcgttca tatacttaac ttcttctata tattttcctc taggatatga
    25441 cgaaatatat acagaataat agtgtcgtgc agaaatataa tctcgttgat gaaaattact
    25501 agatgcaatt aaatacaaga tattttcagc tttttctgtg cctctatata ctggagttat
    25561 atcttgtaat agggttaaag aacgcatata atccttcttt gcaaaatact ctaaggctgc
    25621 ctcatattta atagctggat ctgggttttt gagtaatttt tggtaattac tgcatgatga
    25681 caatagcagc agcattacac ttataaaaag gatataaaat ttatgcataa gtctttttga
    25741 tgaataatct gcaaacttac atatttgtac tgaatgcaga aaataaaaaa cgttttttta
    25801 acaagaattc tattatttcc atttataatg ttgttttcta tgtattaacg acctgcacca
    25861 tacgtatagg ttaaataatg gtagtaatat atcaaataat gggatactac tgatgtattt
    25921 tctttctccc aaaatagttg cagcatagtt aatgattgtt atttgtgcta tatagcgtac
    25981 gaatatgaac gacccaacta caatataagt tgtatgagag ggtaatgcaa gcatcagggc
    26041 tattgtgctg atataaaaaa gtccacgtga aaaaacttct gttcctaaga tgaatttact
    26101 cctccatttg tagtattttg atgtggatat atgtcgttgt ttttggttaa accacctttt
    26161 taaagatgta tttggttcag atacggtaat actgttggga tgtatctcaa ttttcgtatt
    26221 tgtcttggaa gaagcttcac taataaataa atcatcatcg ccagattgaa tattgagatg
    26281 agatgcaaac cctttgtttt tataaaataa ggatttttta tatgccatat ttctaccaac
    26341 tcccatatag gttttttttg ccagagcaaa acttaaatat tgtattgcta taaataatgt
    26401 gtcgtatcct atgaggtgat ttacaaatcc ttttgtttta cgataggctc cgtatccgag
    26461 tacaatttct gtattatcat caaagttgcg tactattttt tcaatccaat ctttactaat
    26521 agtatgacaa tcagcatctg taaataataa aatttcattt ttagcggctt tgatgcccaa
    26581 tactagtgct atttttttag aatcaatata ttttgctgct tggggcacaa aggtagtgcg
    26641 taaatgtggg tatgtatgct ttaacaccat taatatatct tcgctatcgt cagtagatcc
    26701 atcgtttacc acgattactt caaatgacgg atagtcttgt tctagaatag aagggagaaa
    26761 tttttgtagg ttgtccgctt catttttagc acaaattatc acgcttactg gaggaagtgt
    26821 agtattgaaa gttatctttc ccttttttat gctgttccag tatgtaagta gtttgctata
    26881 gcaacaggta tagtaaatta gttgcactac aaatgcaatg aataaacaac taattacaat
    26941 aagttgaaat agcgaaaatg atacgttaaa taatgaatac atactgcata ctgctattat
    27001 tgaaaatctt cggttaaata tacagtcgga tatggtcgca aattgtagcg agatgccatt
    27061 acttctccat aggcacctgc tgaacgtaaa gctactaaat ctcctctttt tgctcgattt
    27121 atctccatcc ctttagcaaa tacatccgat gattcacaaa ttggaccaac gatatcatat
    27181 ttctccattt cttcttccga agtaatattt tcaattaagt gataagcttg atacaaggca
    27241 ggacgaatga gttcagtcat tcctgcgttg agaatggcaa attttgtatg agtaccttct
    27301 ttaatatata gtactttggc tattaacgaa ccacattgtg ctacaattga acgtcctggt
    27361 tcgatatgta actgttgatg aggttgtaat gtgagatgtt gggttattac gtttatataa
    27421 tctttgaaat ttggaatggg attttcattt ggttgttgat agttgatgcc aagtcctcca
    27481 cctacattta tatttttaat aacaattttt tgatcaagca tccattgttg aatctcattt
    27541 atacgggtgc ataaactttt aaagttattc atttccaaaa tttgcgaccc aatatgaaaa
    27601 tgcaatccaa tcaatttgat atttgcttgt tttttaacca catcaattac tttttctaaa
    27661 tcagataaat tgatgccaaa cttattttct gccacaccgg ttgtaatgta atggtgtgta
    27721 tccactttta catttggatt aatgcgtaag gctattgatg caattttttg ttgtttatga
    27781 gccaactcat tgataatttc aatttctgga atagattcac agttgaaaca aaaaatatta
    27841 ttttctagtg catagtttat ttcccaatca tctttaccaa ctccagcaaa tacaatttta
    27901 gatgcaggaa atcctgcatt taatgctgct ttaatttcat ttccacttac acagtcagca
    27961 ccaaaacctt ctttggcaat aatgtttaat attcgatcat tggcatttgc tttaactgca
    28021 tagtgagcct ctatattgtg cttgttggtc tcttgtttta atgactcaag tgttgctttt
    28081 aataatacag tatcgtaata ataaaaagga gttgtcagat tgataaactt atttgttggg
    28141 aataaacctt ttttcatttg aaacgatgta actagatgat taattgttaa ataatttgtt
    28201 acttagagcc ttcaatgctt ttattttatc ttgtgcatca attaaaaaag aaatattgtg
    28261 atcacttcct ccgtacgaaa tcattctaat cggaatattt tctaaagcag atactacttt
    28321 tgcttggaaa ccaatatttg atgagggtag atcacctact acacagataa ttgtcatatt
    28381 tttatcaaca cttactgtcc caaacttttt cagttcatct acaattggat ctaaattttt
    28441 agtattgtca atagaaacag acacacctac ttctgaggta gtaatcatgt ctatgggtgt
    28501 ttggtagctt tcgaaaattt caaacacttt acgcataaag ccatgggcga gcagcattct
    28561 ccctgattta attttaatag cggtgatatt gtcttttgct gctacagctt caatttttcc
    28621 ttttgtgttt tctgttgaaa taagtgttcc ggctgctttt ggatccgttg tgttcagcaa
    28681 tcgtactgga atattttcta attttgctgg taaaatgcaa gtagggtgaa gtatttttgc
    28741 tccaaaatag gcgagttctg ctgcttcttc aaaatgcaat tttgccaccg gttttgtatt
    28801 ctctacaaaa cgagggtcat tgttgtgcat accatcaata tctgtccata tctgaatttc
    28861 atcggctaaa attgcggcac caataagaga ggcagaatag tcgcttccac cacgttgtag
    28921 gttgtctact tccccatagg cgttgcgaca gataaatcct tgagttacaa aaatctctgt
    28981 atcagtatat tggcttagtt gtttggctat gtgttctttg ataaatgctg catcaggctc
    29041 attgtttttg tccgtacgca taaattctag agctgggagt agagttgatt ttattccttg
    29101 ctcacacaaa tagaaatgca tcatattggt tgacatcatt tcaccttgtg ctaaaatctc
    29161 tttttcgtca aataaactaa aaacgggcga tttgctaaaa ctacgtaagt atgaaaaaat
    29221 ttgcttaata gcttctgttg ctttttgttt atattctgtt gttgtgtata gttcattaat
    29281 tatcgctagg tatttttttt cgagtgtatt taccacctca ttagcactac cttgattttt
    29341 tttgtataaa taatctgaaa tttctactaa ggtattcgtt gtgcctgaca tagctgataa
    29401 aacaacaatt ttttgacttc cgtcgcagat taattttgct acatgttgca tgcgctgtgc
    29461 ggatcctaca gatgttccac caaattttaa tactttcatt ctgattaagc gttaatgttt
    29521 atttagcttg aaattttgga gtacaaaggt aatcatttta ctgaattagc cacttatttt
    29581 tgaatgtaaa aaatagaaaa ttgaatagat agttacaaga tgaggactta gaaaccaaaa
    29641 tatcctattt ttgacagcaa aaatgctaca aaaataggat attgttagag gaatcaggtt
    29701 agttgagctc cttggatatt gttatagcgg caatggttcc atctgccaca gcagtggtaa
    29761 tttggcggta ctttttgctt acgatgtctc cgatagcata cacatcagag attgaagttt
    29821 tgcggtcttc atctgtttgt atgtatcccc aattatctaa agctggggta gcgttggtgt
    29881 taagaaaagc aaggtttgct ttaaatccaa taaatacaaa aaccccatca gaagctagtt
    29941 ccgacatttc tttggtgcgt aagttttcga tggttgttac cattttttcg ccacgttttt
    30001 caaatttacg gggttcgtgt tcgtacttaa ctgtaatttt ttcattagct tgtagttgtt
    30061 cgatagctgt tttgttggct gtaagtgcat caaactgatg aatcatcgta attttgctag
    30121 caaatttgct aataaacagc gcttcttcta cggctgagtt tcctccgcca attactgtta
    30181 cttctttgtc aacaaagtat ttagcgtcgc acgtagcaca atatgaaatt ccttttcctt
    30241 tgtattcttt ttcgcctgga gcattcattg tgttggggct agtgccagta gcaatgataa
    30301 ttttttttgc tttgatggtt tctaattcat caattacgat ttcctttttc atcaaatcaa
    30361 ctgatgttat atctacggct actttaaaat cgatgcccgt ttgttttgct tgttcgctca
    30421 tatagtgtgc caacatatag ccattttgtg cctctacaaa gccgggataa ttagatactt
    30481 ggtgtgtgat gcccatgtat cctcctggca aagctgggtc gatgagcacg gtttttactt
    30541 ttgcttgtcc taaatagatg ccagcagtta agcccgcagg accaccaccc aaaatagcca
    30601 catcgtattc acttacactt cgttgttgtt ttttttgtat ctcagcaatt tcattctcag
    30661 ttaacatttt gtttaaacga gctgtaattt ccgatttctt tattccaccg ctgatggtgt
    30721 caagaatttg cttgccgttt tcaaaaaata aaagtgtggg agacgaggag acgcctagtt
    30781 gagtggcgag ctctctgttt ccttgtcggt aaatttttaa gaatttaatt ttgtgtccgt
    30841 acaattccgc tacagcctcg tattttggaa ataaagcttc gcaaggagga cattctgttg
    30901 aataaaagtc aactacgact ttttctcctt ttaagacctc ttggtcaaat tcatttgcag
    30961 taatttcttt catattctat tgtgttttat taatttttgg tatcaatggg ggtatttgag
    31021 aaataatatg ctgcacctaa cgacagtagt tcttttatgg gtctacagtc ggggctattt
    31081 acatgcccat gtttgttttt tgcaacagaa gcacagcctc cgccacaagc cagggctacc
    31141 tcacaatttt tacaagcctc aatggtagtg atgtctctgt tttgccattc ggcaataagt
    31201 gattcattat gagtgatgtg tgggtaaaag gtgcccagct cttctccttt tttgccgaca
    31261 gtagcagtac acgaataaat tgtgcctgta aaatcaaatg cccattcggt tttacaagct
    31321 ggacacgaat caaatagggg agttggtagt tcttcgtttt cagcaatgta ttttgatata
    31381 gagaaagccg gtttataaaa ttcagcaatg tgtggatgtt ctttgagcaa ttcatatatt
    31441 tttttgtaaa gagtagccct gtcgaataat ttgtcaggag tactattaca aaaatgtaac
    31501 tcgtagtttc tacctatctg agtcttaaaa tagggcgatt ttgtccaccc tttgtcaata
    31561 gaaaattgag aaaactctac taaattatct atgttctctt tatcaactac catacgtaag
    31621 tttatgggca tttcagcttc aatgcatgca tctataccct caactatttg attgaaagtg
    31681 ctttgtttgc cttttaaata ccttctgttg ttgtgtacct gctcggttcc gtcaagcgta
    31741 acttgaattt cgcgaatacg tgcttttttt agtatgtcta tatacgaaag caaggtgtag
    31801 ccatttgtta caatggcaat atcaagctga gccgtggtag ctttttttat aaaataggca
    31861 ataagttcgc gttgttgttc gtttggcaac aagggttcgc ctccaaaaat cgtgatgtac
    31921 ttttttttct gtgcaaattg tgtggcaaca tacgcaaaaa atgcatctat tatctccgtt
    31981 gttagttttt gctgaatggg attatatcct tcttgatagc aatacgaaca agcaaaattg
    32041 cacgaataat tgggcacaaa aaacaattga gtttcttcat cttcacgttt gtcaataaaa
    32101 tcgaggtacg ctaatttgaa agttcgtttt tctgtttctt catctacgat atacttgttg
    32161 cttgtaaaac tgccttccac atctatgttg ttttttaagc atttggcttc atttttagtg
    32221 aggatgtcag ccgatttgtg tagcgtatta actacaaaat aatcagttga gtcagtaatt
    32281 ggaacaagta tggtgtgctt ggattttttc atgcaaaaaa gtggattaaa ataggggtgt
    32341 taaaaatcta actatgaata aaatatatcg gggaacgaat tccccgatac ggttactctg
    32401 catagtacac ttagtcgtga caaccagcca ctatgctcga aactttagca caacattgag
    32461 cttgtttcga tgagttgctg ttacatgcac agcgtttttt cactaaactt ttcataaaaa
    32521 aatagtatta aattaagtaa aagtgaaata tccgttgtta tcggatttta tctattggtt
    32581 tttttcctgt tactgtaaaa ctggctactt cagctttccc tttggcatag ggcaaagatt
    32641 cttcgagtat attgatgctt tcaaaaccag tttcctcaag tgtatataaa taagtagcac
    32701 gtgtagatgc acctgcccaa cattcagcta ctgcttgtgg gtcgttttta tattcagccg
    32761 caatttcgtg agtggcgtaa atatcactta ccacaaatcg accagctggt tttaaaatgc
    32821 gaaaaatctc agaccacacc gcttgtttat tggaggcatg atttatggtg caattagaaa
    32881 taactacatc tacgctgtta tcttgtagtg ggatattttc caaattactc tgaatgattt
    32941 ttgcattttt tatgtcgaat ttttcaatca acgattgtgc tttggcaatc attccttcgg
    33001 atatatctat accatataca tacccattgt tgcctaccgt ttctgccaaa cgtacaacat
    33061 catttcctct gccactgccc aaatctacac acacttcacc aaccaatgga ttggcatagt
    33121 ttatagcacc tccgcaagac aaacagcacg tcgtttcgct taatgaagaa tatcgtttgt
    33181 tgattgcatc tgttatcata aagatacttg tttattgtaa ataatgtgca aaaatacaaa
    33241 aattatttta tattacaata gtatcactat aaattatttt attatcacta aaaaaatgta
    33301 tatatttgcc agaaattaca aacgaaataa caataatgct ttttaagaac gaaatatctg
    33361 ctgagtttgc acagcaatta tgcgaagatg ctgataaagt gctggagttt gtgtcgcaag
    33421 taaaatggaa agaaggtttt gtgtgccgca attgcaacca taccaattat tgcgaaggaa
    33481 aaaccccctt ttcaaggcgt tgtacgcgtt gtaaaaaaga agaatcggca acggctcata
    33541 ccttgtttca taacataaaa tttccgatta acaaggcttt ttttatagcc tatcatgtgt
    33601 gtatgttagg agatgagttt tcgtcctata cgtattcttt tcagttagga ctcaatcaga
    33661 tgacttgttg gaaattcaga aaacggattc agcactgtat tgctaaggtg gataaacagg
    33721 actcaaacac catacaaggg attttgttac aaacgtatga ataatggcgt gttatataat
    33781 aaaatagtat actaattaaa taaaaaacga tgagttacaa aaatttagac cagcaaaatc
    33841 tatctttttt aaaagagtta ctttcaacaa attctccttc aggttacgaa gtaaacgcgg
    33901 caaaagtatg gcaaaactat gtaaatcaat ttgcagaagt aagtggtgat gtgttaaaca
    33961 attctatagg ggtgcttaat ccccaagcgg aatttaaaat tatgattgca ggtcattatg
    34021 acgaaatagg tttgcaggtg atgtatattt ctaaagaagg ttacttatat gtgcgtaggc
    34081 tgggaggcat cgacttgttg agtttgccag gtagtgaagt ggtagtgatt accaaaaaag
    34141 gtactataag tggcgtgttg ggtaaaatcc ccattcatgt tcaatcgcca actgagcgaa
    34201 ccaaagccat tgagttagaa gatttatgga tagacatagg cgtagattcg gaagaggaag
    34261 ctgctaattt ggttgaagta ggcgatccag tagctattaa atccaatttt cattatttag
    34321 gtcaacacaa aattgtatct aaagggatgg atgacaaaat tggtgcattt attgtagctg
    34381 aggttcttcg taatttatca acacaacaac ttaaagtagg cgtatatggt gtagctacct
    34441 cacaagaaga attgggagcc agaggtgcta ttacaagtac ttttaaggta aatccacacg
    34501 taggtttttg tttagacgtg ggtattgcta ccgatgtgcc aggtatagaa gaacgaaaat
    34561 atggcaaaat gagacttggc ggaggagctt ctattaccca tagtgcagat aataatgtgg
    34621 ttttagtaag gttgttacaa caaacagcac agcaaaacaa tatccagtac caagatgtgg
    34681 cttctaaatc agcaacagga ggaacagata ctgctcatat gcaacttgct agacaagggg
    34741 tagccacagc attaatcagc gtgcccaata gatatatgca tacgcaagta gaaatgtgcg
    34801 atttgagaga tgtagacgaa attatttcct tattagttga aacaatcaaa gtaatagaca
    34861 aagatgctat tgtagatttt gttccatctc atatgtagaa aaaaaagaac aggaaataaa
    34921 atgaattatt tcctgttctt agagaggttc ctggcggatt cgaaccgccg tatacggttt
    34981 tgcagaccgg tgcctagcca ctcggccaag gaaccataat atggtagtgc ggttagcgac
    35041 tgcaaatgta cacttttttt tcagtatagc aataattctt ccaaaaaaac tgaaaaaaga
    35101 tatcttaaat aggtcgtgtg taaagaaaaa aaactatttt tgtgctctat tttctcattc
    35161 aacgaattgt aaatataaat tagtatggca aagaacaaaa aaaatgatgc aactgaaaaa
    35221 gagttagaag caattgaaca cgtattaagt tcatcagagg catttattga aaagcaccaa
    35281 aataaattgt tgataggttt aggtgtggtt gttgtaattg tgttgagtta tttggcgtat
    35341 tctacttatt atgtagctcc taaagagaaa caagctgcag aaatcgttgc tgtaggtcaa
    35401 aaatactttg cacaaggaaa ttacgaggca gtgttacatg gagacagtgt tgaatttgat
    35461 ggattaatag cattgacaga taaatatagt atgacaacaa gtggcaatat aggtgctgct
    35521 tatgttggtt tagcgtatta taaattaggt gaatatgaca atgcaatcaa atatttgaat
    35581 aaaacgtcga ttaaggatga tgtgttggca tatactactt tgggcacaat aggcgatgct
    35641 tatgtacaaa aaggtgaaac acaaaaaggg atagagtatt tcttaaaagc ggctaaaacc
    35701 gacaatgcga tggtgggtgc aacctacttg ttaaaagcag gaaaggcgta tgaaagttta
    35761 gcaaaatatg acaaggctat tgaggtttat cagttgataa aaacacagta tgcaagtcag
    35821 ttgccaggtt ttgctcctat tgaagatatt gataagtata ttgagaaagc aagctcttta
    35881 aaataataat ggctacacaa taccaaaatt tatcatctta tgatgtggct tctatgcctc
    35941 taaaagatat cgtgtctgta cagaggtatg cgattgtggt agcagactgg aatgctaaca
    36001 ttacacatgc tttgctagaa ggtgcttata catcattaat agaaaatggg gtattggaac
    36061 ataacattaa agtaatacat gtgccaggtg catttgagtt gacatatgca gctaaagagt
    36121 gtcaaaaaca aaaagtgttt gctgcagtta ttacattagg gtgtgttata caaggagata
    36181 cacctcattt tgactatgta tgtcaaggag taacgcaagg tatttctata ttaaatacaa
    36241 aaaagagtgt tacgccagtt atttttgggg tacttacgac tttaacaatg caacaggcat
    36301 tggatagggc tggtggaaaa gacggaaata aaggagtgga agcagccata acagcgataa
    36361 aaatggctaa tatattttga tgtatcaata atatatagta tctttgtgtc gcaaaaaaaa
    36421 gggcagttac cagagtggcc aaatggggct gactgtaact cagctgtctt tcgacttcgg
    36481 tggttcgaat ccatcactgc ccacaaaatt gcggaaatag ctcagttgat agagcattag
    36541 ccttccaagc tgagggtcgc gggtttgagt cccgttttcc gctctatgaa gggatgataa
    36601 tgaaacgtat catcccttct ttttgttttt atatttcaaa cgtttgcggt acttgttgta
    36661 gcctaaatat tgtctaagtt attaacaatg aggataattt tatgaataaa aaacttgttt
    36721 gttcttgcaa aaatagtatc tttgccctcc gttggaataa aatgccaaat atagtaatct
    36781 aaatagtaga aaaaattatt tcttaaccta aatgaatatg aaactaaaag ttagtaatgt
    36841 aaattatcct aattggagag caattaccgt aaattttaaa gtgccaaaag aatttgagaa
    36901 attacaagaa atttcaaaaa atttatggtg ggtatggaat tatgaagcaa ctgatctttt
    36961 taaagaaatt gataaagatt tgtggattga ggcaggagga aatcctgtgt tgtttttgca
    37021 gaaacttagt gctgaaaaat acaaagaatt agcaaaagat aaagctattt tgaaaaaaat
    37081 agataaaatt tatgctgatt ttaaagctta tataaatgtt aaaccaagta caactattcc
    37141 ttctgttgct tattttagca tggaatatgg tttaactgat attttaaaaa tttactcagg
    37201 tggtttgggt gtgttagcag gtgattactt gaaagaggct agtgattgca atgtagatat
    37261 ggtagctgtt ggttttatgt atcgttatgg atactttaca caagctttat ctattaatgg
    37321 cgagcaagtt gcattgtacg aagctcaaca atatgagtca ttaccaatgg aacaactcct
    37381 tgataagaat ggagatccag tagttatttc agttccatat ccaggtagaa ctgtgtatgc
    37441 taatctatat aaagtagctg ttggacgtgt tgatttgatt cttttggata cagataccga
    37501 attgaataat gatttggatc gtactattac acatcaatta tatggcggag acaacgaaca
    37561 tcgcttgaaa caagaaatta tgcttggtat tggtggtatt gctgctctta aagctattgg
    37621 cttagaaaaa gatgtttatc attgtaacga aggtcatgct gcatttttaa atgtacaacg
    37681 cctaattgat ttgatgcaag ataaacaact ttcttttaat gaagctaaag aaattgtacg
    37741 agtgtcatgt ttatttacaa ctcatactcc agttcctgct ggacacgata cattcgaaga
    37801 accattattt gagcgttata tgtttgattt tgctactcgt ttaggattat cttgggatga
    37861 ttttatgaat atgggtcgcg aaacgcctgg tgatttgaca gaaaaattct ctgtaacagt
    37921 atttgcttgt aatactgctc aagaagtgaa tggtgtaagc tggttgcacg gaaaagtttc
    37981 tcaagatatg ttcaaacata tttgggaagg ttattttgca gaagaatcgc atgttagttg
    38041 ggttactaat ggtgttcatt atccaacatg ggcagcatct gaatggcaaa aagtgcacga
    38101 aaaagcttta ggtaaagagt acatgtctga ccaatcgaac aaagctattt ggagcaaaat
    38161 atatgatgta gacgacaaag aaatttggga tactcgtcaa gctctcaaat taaaattggt
    38221 tgaatatatt aaagcaaaat ttgcttctaa ctggttgaaa aatcaaggag atccttctcg
    38281 tattgtaagt gtgttggaaa gaatcaatcc taatgcttta accattggtt ttggtcgtcg
    38341 ttttgctact tataagagag cacatttatt gtttactgac cttgaaagac tttctcgcat
    38401 tgtgaataat cctgattttc ctgttcaatt tatttttaca ggtaaagctc acccagctga
    38461 tggtggtggt caaaaattaa ttcaacgtgt tgtagaaatt tctcgtcgtc cagaattctt
    38521 aggaaaaata attttcttag aaaactatga catggcaatt gcaaaacgtt taatttcagg
    38581 tgtggatatt tggttaaata caccaactcg tccgcttgaa gcttctggta cttctggtga
    38641 aaaagcagaa atgaacggtg tattaaactt ctctgtactt gatggatggt ggtatgaagg
    38701 ttacaaaaaa ggtgcaggtt gggctttgac agacaaacgc acataccaaa atcaagaaca
    38761 ccaagatcaa ttagatgcag caactattta cagtatgctt gaaaatgaaa tagtaccatt
    38821 gtactttgca aaaaatagca agggttattc tcctgaatgg gtgcaatata tcaaaaattc
    38881 atttgcgtta attgctccag aatttacgat gaaacgtatg ttggacgatt atattgaaag
    38941 attctatact aaattagcta aaagaggtaa atctttaaaa gcgaaggatt ttgctaaagc
    39001 aaaagaaatt gcatcttgga aagaaaatgt tgcttctaaa tgggatgcta ttgaagttgt
    39061 ttcggtagaa ctatcagaaa accttcatgc acaagctgaa gttggtcaat ctcaaaaagc
    39121 tactatcgtt gttgatgtaa aagacgcatc tttagtaaat tcaattgggg tagaagaagt
    39181 ggtaacacat cctaataaag aaggtgtgga tactttcttc tcagctaaag aagtaaatct
    39241 agtgaaaaca gaaggtacct tgcttacttt tgagatgaat caaaaagtgg aaaatggggg
    39301 atcgtttaaa tatgcattcc gcatgttccc taaaaactca gatttgcctc atcgtcagga
    39361 tttctgttat gtacgttggt tttaattaaa caaataatac tattttctca agaagctgtc
    39421 tcaaaaggac agcttctttt gttatgccaa gttatgattt tctgtagagg atagataagt
    39481 gagattagtt aattatatag gtattatata accaaaataa tacataaaat aaaaatagag
    39541 gatggaacgt attcgtcatt ccatttgctt cgtatggtaa ggtttatttt atttgttatc
    39601 aatacgttta atcaaataga taatagaggg gaaatgatca ctatacccgt tttgatagac
    39661 tccaccagca tgagttctaa aaggataacc cttatattga ccttcttgac gaattaaaaa
    39721 tggtgcttga aaaatttcag ctttccaaaa tgtgagccct gctttgtctt tgccgattaa
    39781 acctgaggaa acaataattt gatcaaacag gttccaggca tctccataag ctaatgagcc
    39841 tatgccttta ttgtaaagct cccaagtggt attatacaat ccgccttcgg gtgtttcgct
    39901 ctttttttgt tttgcattca gtacatcttt aacactttcg ttgattgggt cgtcatttaa
    39961 atcacccata atgataattt tagcattttt gtcgatagta aaaattgaat cagcaatatg
    40021 cttggttagt tgagccgctg ctactcttcg tggacgtgaa ttgacttctc cacccctacg
    40081 cgaaggccag tgatttacga ttacatgaat ttcttcgcca cttaatatgc ctgacactac
    40141 taattggtcg cgacttctac gagatgaatc actgcttgtg taataaggat aactttttac
    40201 attggttacg taaaaatctc gtggattata tagtaatgca caatctactc ctcgtgggtc
    40261 tcctccatca atatgaataa tttgatagtt tatgtgttta atagcttctt gtttaactaa
    40321 atcttctaat actgaacgat tttctacttc agctagacct aaaatggagg gtcccatagg
    40381 agaaagatta cttcctattt ttgatatagc ataagacata ttcttcattt ttgcccaata
    40441 ttttttgcta tcccattgca tagcacctgt tggtgtatat tcatggtcgt tgggttctgg
    40501 tatcgtatca aataggttct caacattgta aaatgctaca ctatataatt cataatttat
    40561 tttttctttg cctataagtt gggtaataag aaagaataaa cttgcaacta agaggattaa
    40621 ttttttcatg tgtatgcttt tttgtgttta tgaatagtaa tggtgtgtaa ttgtacgttc
    40681 ttttctgtat atttagagct ttatgcataa aaaagacaaa aaataacgca atcaacttca
    40741 caaatttgct tatttttttt tacataacca acagatacaa ctgttaattt ttggagaatt
    40801 ttataaacaa ttgttttttg tgctattatt ttacttatat ttgtctttag ctaattgtat
    40861 cgttttagga gatatacaat agtagctagt tgaggtatat gctatttgat attgtaatgt
    40921 tacattttta attctagttc atgaaaaaaa aaatactctt attattgctt attgccaata
    40981 gtgtattggt aatagcacaa actggtattc aaggtgttgt acgcaatact actaccggag
    41041 atggtattgc cgatgtgcaa gtcgtactaa aaaagaataa tgtatttgtg ttaacgaacg
    41101 aaaaaggaga gtttaccttt aatgagttgc aagcagggag tgatgtgctt gaaatagttc
    41161 atcctgtgta tgttgccaaa ccattacaag ttaccattgc tgcaaacaga atatccaata
    41221 tcgggattat tcgtgtacga gacgaccaaa gtgtggtgcg tgaggtggca caagatatag
    41281 cgaatgtaat tacgttggat tcggaactag caactggaga cgaaacaaca ggttctgcaa
    41341 cacggggtac tgctttatct gcacgtgggg atgtatatgc tgcggctact aattatacgt
    41401 ttagtgcaat gcgtttcaga acacgaggat atgacaatac ctatgaaaat acgtatatca
    41461 atggtgttaa ttttaattcc ttaccccgtg gcatgtttaa ctattcgatg cttggaggat
    41521 taaatgatgc gatgcgtaat aaggatgtga atagaggatt gtctcccacc gcttattcgt
    41581 atggaaatat tgctggtgta actaatattg ataccagagc cagtcagtat gcattaggta
    41641 cacgcaccag tgttgctttt agcaatcgtt cttatacctt gcgggcacaa gcattccatg
    41701 cacgtgattt gggcaacgat ttgtacttgg ctgtatcggg tgtagcacgt tgggctgatg
    41761 aaggtataat agaaggttcg ttttataatt cagcagggct ttttactgct ttagaaaaga
    41821 aatttgctga taaacatagc ttttcactca cactttttgc ggcacctacg caacgaggac
    41881 aacaagccgc atctttacaa gaagtatatg atttggcggg tagtatttat tacaattcgt
    41941 attggggata tcaagatgga caaaaacgta attcgcgtgt agtgaaaacc attgacccta
    42001 ctgctattgt gagctacgaa tggaagattg atccacagca agttttacgt gctgggtt
//
LOCUS       DAOSRP010000002        38110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1202_length_38110_cov_5.181763, whole genome shotgun sequence.
ACCESSION   DAOSRP010000002 DAOSRP010000000
VERSION     DAOSRP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_38110_cov_5.181763"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3866
                     /locus_tag="PLJ82_00215"
     CDS             <1..3866
                     /locus_tag="PLJ82_00215"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOR39968.1"
                     /translation="IMRNFLKLYGFLLFFVLGIIGLNAQSVSINEIMSSNSKTITDED
                     GSFEDWIELYNYGDTPVNLHQFGLSDNANNRFKWTFPEIMLPPQEFLLVFASDKDRKF
                     VPMEEQVLVSSTTIWKYVDAGEDLGSDWKEIDFDDTEWKTGTAPLGYNTNGNERYATV
                     LDYGSDKSNKYISYQVRTLFEKPTADFRQFTIQLVADDGIIVYLNGKEIIRQNMPDGE
                     IAYTTLAQSGITSIDTINYQLDGSLLQNGTNTIAVSVHQVTLNSSDIYFDMKLWTNKQ
                     YPHANFKIASEGETLFLTNKENSIISNVASVYIPSDISYGRTIDGNGDFAFFATPTPG
                     TSNITESSSEVLVPVNFSHNGGVYTEELSLTLSHPDPEATIIYTIDGSIPSINNLGGT
                     TYLYKNEFPNLRESPEADSLFRSFQSYEYTGEEILVYDKSAEDNQLSNITTSVIHTYL
                     PDNPIRKGFVVQAKAYKNGAISSHISGQTYFIWEEGNPYNVPIISLQIQEDFLFDYNK
                     GLYTPGADWDNALALDEDANPCLFGNYISTREYPAHVEIFDPLSPESDVNQGVGVRIH
                     GGCSRLTPIKSLRLYARNEYDRMGEINYNPFSSIPYQASNPSNTLFKRMLLRFSTSGG
                     PQIVDIVTHKIMESVYPGVQRTRHAIEFINGEFWGITAIRDRFDQYHLAYNYNLDSDN
                     IIFYTPWGIVDEGLPEDKELFTSMYNYISTADLSTEIAKAKVESLLDIQDYFDYYIMN
                     IYFGKVDWYGDRHFAFWRVRTPVNSQFGDGRFRTFTWDFDTATSLGAEYNMLHEALHE
                     YHPQHIAMINNLLTNEQFKYDFINRFADHLNTTFLPERVENIVHQEASEFSSYLPEHN
                     DRWPNIYPGPEDINDRLIQYAVDKSTIQRTQIMDEFGLSGTYALTIDVSDKTAGSVRI
                     NSIDIQSTTPGVSDNPYPWSGVYFSGIPVKLTAIPKEGYRFSHWSGVINSTSNSILVD
                     ITNDDSIQANFVPIGNDGDLIYFWLINNTIANDTPLSEIPATFSKMPEEYASLEFSSC
                     LIGYPFNSTHSLWRKASMERKNAPTSINYFPEANNNRTYGTFTMRALQIKEPFQQNDI
                     KNTLYLNIPTTSYKDIIVSFAAKNEGAANGFEVDYWDINSGKWEQTDMNVSTFNLYSE
                     YQRYEILFKNVLTANDNPDFRLRIGFTGATPSTEDNGNNVVYNNFAVQGTPLLITRIL
                     ELDNSAILTTIFPNPASNYVKVYCSLPIIKSTLINTLGITIPTHTITSTSTEITLDIS
                     QLSLGGYILLVETAEGISPVKFIKR"
     gene            complement(3871..5310)
                     /gene="rpoN"
                     /locus_tag="PLJ82_00220"
     CDS             complement(3871..5310)
                     /gene="rpoN"
                     /locus_tag="PLJ82_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOR39969.1"
                     /translation="MALKQQFQQKLQQKLSPLQLQIIKLLEFSVVELEEKIKTEIEEN
                     PALEEGAPEEDNSINEEDVEDDEDDTFGINDYMQDDEDGDYMPQDPNNMPASVYNIPY
                     SEGESFHEYLLNQLHLLPLSKTQLLLAEYIVGNIDDEGYLRREVVQIATDISVLTGVH
                     YTDSQLYEVLAIVQQLEPAGVGARSLQESLLLQLQRKQLTPSIDLAMQILEDCFIEFS
                     SKHYEKISAKLAIDNESLRDAIDEIIHLNPKPGNGWEGILEKNKEEITPDFILEVQDN
                     QLTLSLNNSTIPMLRVNQTYVNLFQDFVSNKVNQTQSMKDAVLFAKQKLDAAKSFIDA
                     VKQREETLLKVTSTIVNYQKKYFLEGSDSFLRPMTMKDIAELSGYDISTISRVANSKY
                     IQTNFGIFPLRHFFTEGMITQSGEEVSTREIKNILSQTILSEDKSKPLSDDKLTEILQ
                     QKGYNIARRTVAKYREQLDIPVARLRKEM"
     gene            5449..6177
                     /locus_tag="PLJ82_00225"
     CDS             5449..6177
                     /locus_tag="PLJ82_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR39970.1"
                     /translation="MKTIGKIFWGFYLIVFFLPLAILATMLTALTTIVMCGIGNHRIW
                     GYIPGKYWSKIMCALAFVTVEVKGNEYLNPNQSYIFAANHQSLYDVFLIYGWIGFSFK
                     WIMKKELRKIPFVGSACKAAGHIFIDRENPIKALHSIENAKKILTQGVSLAIFPEGSR
                     TRTGNIGKFKRGAFQIAKDVQLPIVPVTIDGGYTIMPFSRIYPVPGKLTLTIHPPVSF
                     SPASHDEELKMIDNIRNTIITGLS"
     gene            6174..8078
                     /locus_tag="PLJ82_00230"
     CDS             6174..8078
                     /locus_tag="PLJ82_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR39971.1"
                     /translation="MKKLFWMYIIVYFLLIPAVLMARGTERTNQLHREAQLALTKGDI
                     NEGIAIYEKIIKQNKNQAEYYWLLDDVLKQLLQNNKRIELLQKLSNNKKANSPEQTKE
                     KLGLAYFDAGNYDSAMLIFNQLKQTRAIKRYISNCEKAKVIRNNPIDITITSMGENIN
                     TMYDNIWPGITADNETFSFTVVEGKTNPFTNPYAIQEDIYQSTKQDECWQKGKRLSAP
                     INTTDNEGSQHFSADGRYMFFVGCNRKEGKGSCDIYYSIKNGDSYSAPINPGEPLNTI
                     YWESTPSFSACGKFLYFSSNRPGSIGGKDIWRCEINILPSGQLTFFNAQNMGDPINTS
                     FDEMAPFIHPDNIHLYFSSNGHPGLGGFDVFQAKKDTLEAWNTISNMGYPINTFGDEI
                     GFIISSSGEKAYLSSNQHIDIDNQHLNRMIYEITLPHSLRPETMQTVKGVVIDNKTNQ
                     PLQANIEIFDTHSQKTISRTTSDNKTGTFTAIIPQKSQVGMFVKKDQYLVYSEQIDST
                     SSDFYTIKLEKIEKGRSFVLRNIYFDFDSAELLSNSYAELHRLLEFLQSNPTLNITLI
                     GHTDNFGNKEYNRSLSIQRARSVYNYLLKKGIDKSRLQFEGKGSDMPIEDNNTDFGRS
                     RNRRIEFVIH"
     gene            complement(8081..8995)
                     /locus_tag="PLJ82_00235"
     CDS             complement(8081..8995)
                     /locus_tag="PLJ82_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR39972.1"
                     /translation="MSWKDYFYFTKQEKRGLSVLIVMLIVLIGTYVGLPYFISNPVVL
                     DDEEVKLQIADFEKTLQEDSASFVYKKQHWSKNIDSTTSILQVFDPNHADSALFLKLG
                     IKPYVISRILKYRAKGGVFRSPTDFSKIYGLPKEMYERLLPYIQIKPIARDKQLVESK
                     ESLPVTTSEITLVDINKADTTMLKTVKGIGSVLSKRIVTYREKLGGFISVEQLKEVYG
                     ITDEVYQKIAPQLTVSQSYTKKISINQATVHQLKQHPYISFYQAQSIYEYRMGKRTKR
                     IENIEEIPFNRDINSDFIEKIKPYVTCE"
     gene            complement(8998..10338)
                     /locus_tag="PLJ82_00240"
     CDS             complement(8998..10338)
                     /locus_tag="PLJ82_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOR39973.1"
                     /translation="MMETRDNFTGKIGIVLATVGSAVGLGNIWRFPYEAGQNGGGAFI
                     LIYLLCVFFLGFPLVMAEFAVGVKAKANVVGSFKLLAPNTKWFLVGIMGVVAGFMIMG
                     FYYVVTGWTAEYFWQAIQGNLLHKSSEEYKVMFDSFIASPIKPVIWTVIMIALVHIVI
                     INGVKNGIEKVSKVLMPMLFVLMIILAIHSLMLPNAMQGIRFMFEPKVENVTSYGILT
                     ALGQAFFSLSLGMGCLITYASYFGKDTHIPRIAFQVTLLDSLVAIVAGVVIFPAVFSF
                     GLQPDEGTGLVFVILPTIFQQMPFPIFWSSLFYLLLALAAFTSVISLHEVVTAFVKEE
                     FSYTRKKAVTLVSVAVLVLAVLASLSLTLLFPVIFDKTLFEWLDFITATILLPLGGVA
                     VSLFVGWYVDKRILLDVLQQEGTWKFRFFKTYIFLLRYLAPVCIMLVFVHNLFA"
     gene            10452..10904
                     /locus_tag="PLJ82_00245"
     CDS             10452..10904
                     /locus_tag="PLJ82_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOR39974.1"
                     /translation="MKERNKRLEAIKEIIAHTTISSQDNLLESLIDKGFNATQATLSR
                     DLKQLKITKILTDDGIYKYVIPFTNYANKTIGNTREIISIDFSGQLAVIKTRAGYANG
                     IASDIDQQLAELILGTIAGDDTILLIPKEGITRNTITTAIRSFMTVIS"
     gene            10926..11564
                     /locus_tag="PLJ82_00250"
     CDS             10926..11564
                     /locus_tag="PLJ82_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR39975.1"
                     /translation="MESDTIKIMVANESHYTYIDEILSTIEESSKDRGTGIAKRSPEY
                     VKNKMAEGKAIIALDGEVFAGFCYIESWGNKQYVANSGLIVNPKYRGHGVAKKIKKFS
                     FDLSRQRFPNAKLFGLTSGAAVMKINTELGYVPVTFADLTDDEEFWKGCQGCVNFDVL
                     QRTNRRYCICTAMLYDPNDPNRRNREEKENEAKKQNKKSVITDFINKFKKLK"
     gene            11577..12779
                     /locus_tag="PLJ82_00255"
     CDS             11577..12779
                     /locus_tag="PLJ82_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOR39976.1"
                     /translation="MKKEKVLLAFSGGLDTSFCAMYLAKDKGYEVHTAIANTGGFSPE
                     ELKRIEERAYRLGAKTHATLDVTQEYYDKSIKYMVFGNVLRNGTYPISVSSERIFQAI
                     ATIEYAKKIEADYIAHGSTGAGNDQVRFDLTFDVLAGHIKVLTPTRDMVLTREYEINY
                     LKEHGYEADFTKMEYSINAGLWGTSIGGKETLKSEQTLPESAYTKQVVKKEEETITID
                     FEKGEIVGVNNVHYTDKIAAINKVEEVGSAFGIGRDMHIGDTIIGIKGRVGFEAAGAL
                     LIINAHKMLEKHTLTKWQQYWKDQLGNWYGMFLHEAQYLEPVMRDIEKFLENSQQNVT
                     GKVIIKLRPYSYTLVGVDSPFDLMKTDFGEYGEVNRAWSADDVKGFTKILGNQMKIYY
                     NVQAKNNK"
     gene            12782..13744
                     /gene="argC"
                     /locus_tag="PLJ82_00260"
     CDS             12782..13744
                     /gene="argC"
                     /locus_tag="PLJ82_00260"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOR39977.1"
                     /translation="MIKIGIIGGAGYTAGELLRLLVHHPQADIVFVNSTSNAGNKLYA
                     VHSGLIGETELEFTNELPLDAIDALFICSAHGDSRKFLDANTIPSKVKIIDLSTDFRD
                     ESNGFVYGLPELNKQRILQANKIANPGCFATVIELALLPLAANGLLTNEVHINAITGS
                     TGAGVKPSSTSHFSWRNNNISVYKAFEHQHLKEITQSITQLQPSFGKAINFIPVRGNF
                     ARGIFASVYTDCSLSLEEAKALYKNYYKDAAFTFVTDTNPDLKEVVNTNKCIVYLEKH
                     GDKLLILSCGDNLLKGASGQAVQNMNLLFGLDEKMGLNLKNIAF"
     gene            13785..14906
                     /locus_tag="PLJ82_00265"
     CDS             13785..14906
                     /locus_tag="PLJ82_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR39978.1"
                     /translation="MKLFDVYPLFPIEIVKGKGCKVYDNNGTEYLDLYGGHAVISVGH
                     AHPTYVQAVADQVGKLGFYSNSVINGLQETFAEKLGIACGYPDYSLFLINSGAEATEN
                     ALKLASFHNGKKKVLAFKKAFHGRTSVAVKVTDSPSYVAPINTGLDVTFLALNDIEGV
                     KKELATGDYTAVIIEGIQGIGGIIEPNIDFLQALRLECTQTNTVLILDEIQSGYGRSG
                     KFFAHQYAGIKADIITVAKGIANGFPFAGVLISPMFKAVHGQLGTTFGGNHLGCAAAL
                     AVLHIIEKENLIENAKQIGDYLLHELKQIPQIKEVRGKGLMIGIDFEQAVKEIRNKLL
                     VEQHVFTGVAGTHTIRLLPPLCLTKEEATEFLLKLKQCL"
     gene            14956..15477
                     /locus_tag="PLJ82_00270"
     CDS             14956..15477
                     /locus_tag="PLJ82_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOR39979.1"
                     /translation="MISKKMQDAINAQINAEFWSAYLYLSMSNNFAAKGNPGFANWFE
                     IQFKEEQDHAMIFGKHILARGGKVSLAPIKEVKTEWATPLEAFEETLKHEKVVTGLIN
                     NLYKIAVEENDYATQSMLKWFIDEQVEEEETAQELIDSLKMIANNGFGIYMLDKELST
                     RTYKTPSPLVAKA"
     gene            15569..16372
                     /gene="proC"
                     /locus_tag="PLJ82_00275"
     CDS             15569..16372
                     /gene="proC"
                     /locus_tag="PLJ82_00275"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOR39980.1"
                     /translation="MKKISIIGAGNMGGAIARGLSQGSIFSAQQITVSDISTENLQAI
                     KTFNPLINTTLSNVDGVQHADIVVIAVKPWLTETICNEIKSVLNYDKQVVVSIVAGVS
                     FEQLHTFLKKEGESSPTIFRVIPNTAIDVLQSVSTIASSHANKQQEEEIVRIFNELGK
                     AFLVSSEQQLNAFMSLSSCGIAYAFRYIRAAVEGGVEMGIYPETAKQVVLQTLRGAID
                     LLEATNAHPEAEIDKVTTPAGITIKGLNEMEANGFSNAVIKGLKASYIK"
     gene            complement(16379..17950)
                     /gene="nadB"
                     /locus_tag="PLJ82_00280"
     CDS             complement(16379..17950)
                     /gene="nadB"
                     /locus_tag="PLJ82_00280"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOR39981.1"
                     /translation="MVRKYDFIVIGSGIAGMSFALKVAHKGKVAILSKTRLEEANTNL
                     AQGGIASVTKDSDNFEKHIQDTLICGDGLCNEEVVRMVVENAPSQIDELVKWGVDFDK
                     GDEGNFDLHKEGGHSDFRILHHKDATGAEIQKRLVRQIKKHPHIDVFENHFAVEIITQ
                     HHLGMLVKHTTPNIECYGAYVLNEKTRHVHTFLAKVTMMATGGIGSIYQSTTNPSIAT
                     GDGIAMVYRAKGLVSDMEFVQFHPTSFYNPNERPSFLITEAMRGYGAVLRTRKGRQFM
                     HKYDERGSLAPRDIVARAIDSEMKLSGDDFVYLDVTHKSAEETKAHFPTIYQKCLSLG
                     VDITKDYIPVVPAAHYLCGGIEVDVHSQSSIRNLYAAGECSRTGLHGANRLASNSLIE
                     AVVYADKAATHALGRIEGVVFNEQVPEWNDEGTSLNEEMVLITQSAKEVGQIMSNYVG
                     IVRSNLRLKRAMDRLEILYKETEDLFTRSVVTKDICELRNIINTAYLVIKMAMQRKES
                     RGLHYTLDYPDKIEL"
     gene            18114..18740
                     /locus_tag="PLJ82_00285"
     CDS             18114..18740
                     /locus_tag="PLJ82_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR39982.1"
                     /translation="MNRIYKISSLLIFLFIINLIQSCGPSMDGYIGFDITHIQIQNLS
                     NTGSDAVVTTENTMLPSAVAFKVRLYEGSNSVRLRMQPQTNFGFSSCVALSPPDFYYK
                     SNQLIQNIRVVTLVDYSTSIVANTDVSDKFLVYPIVPAISDFLYVSIPEFCTLTTDQI
                     LMSPSIEFHLMCKETLSLNNAQFRIIVELSNGQELSIDTALLDLVTND"
     gene            18745..19590
                     /locus_tag="PLJ82_00290"
     CDS             18745..19590
                     /locus_tag="PLJ82_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR39983.1"
                     /translation="MKRLLIALILTCIPAHYVFSYTLTGWFEESKISVFGGVATHHIE
                     KRNSTEDVNLMNYLSYRNEFYLGVDAHVYLHEQWQFNIKTYLNSSLRTDDVFAKLIYM
                     PHEKIGVTLGYASIPVQYGLYEIHEQPQYYTTIPNSDDVYDFAKNELFLAGLAFPFEG
                     GKFSGTIYLQGGVSYLPNDFSFSWQKKYDSNERRLVEYDIPESYAFIIQPEVEIRWEI
                     AKPTTDTSIGLQFQGKWNYSKRNIDYTRNIYTWTTDNKQTEIISHPKQSIEGVDWNVG
                     LFYIF"
     gene            complement(19597..20145)
                     /locus_tag="PLJ82_00295"
     CDS             complement(19597..20145)
                     /locus_tag="PLJ82_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR39984.1"
                     /translation="MNSFYNLVSSRQSDRAFLPKGIEDEKLQRILASAQLAPSACNAQ
                     PWHFVVVNETDKRLQIADAMASKVLAMNHFTKQAPVQIVVVEEKANISSSFGGWAKSK
                     HFPHIDIGIVASHICLAAADEGLGTCMIGWFDEKKIKNILSIPDEKRVLLVIVMGYSA
                     QETRPKKRKKLNEIVSYNSYKK"
     gene            complement(20199..22184)
                     /locus_tag="PLJ82_00300"
     CDS             complement(20199..22184)
                     /locus_tag="PLJ82_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOR39985.1"
                     /translation="MAQNTYLETIDRIVENLEMSDDEVVLDDTYEDLLRLAENPLNVN
                     TCTREQLEQLFFLSPYQIENLLYYRYSYGDLYTVYELQLIDGFDEETIQFLLPFIYVR
                     KSELQSEGITLQNAFRYGKNQVLLRTDCTIQKKKGYTHLATDAQRYVGNPLYTQFRYR
                     FSSQYLSIGVSMEKDAGEPFDWHYNKGFDFYAPYLFISSLWKFKHIALGHYKLNFGQG
                     LVLQSSQRFGKSSLFSNTIYKEGISKSASLDESLYFNGLATTIGHKKLEITAFYSNTP
                     LDGTLEYDLNNLLGLSSIKTDGIHRTENDFVKKNVARQMVIGGNIQYHFPFLKLGITG
                     FSYSLDKPSFPSSKPYAIYEFAGKHYSTASIDYRARFHQFNVAGEMAIDKNGAMATVH
                     TLSFQPISRFGLVALYRYYQPEYSSIFGNGFSQSSSTNNEEGFFMGTELLPFRKWKLV
                     SYLDVFSFPWLKYLVNTPSRGYQGFIQATYTPTRNTTFFCQYKSTSKQRNVLLEEMAT
                     PMVNPYTKNEWRFQIDTNPFKHLHCKTGMHSNEYASQLNHPTYGFLLYQDVGYEFSSI
                     PLLLNARYALFDAINYDNRLYAYERDVLYAFSVPMYYGQGMRLYVNVVYKPFPQLTWY
                     AKFAHTWYADRNEIGSEGETIYGNQKTDIRILVNWKF"
     gene            complement(22257..23018)
                     /locus_tag="PLJ82_00305"
     CDS             complement(22257..23018)
                     /locus_tag="PLJ82_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR39986.1"
                     /translation="MQQIENPRSLALGKSYVMLLDNNYALANQASASFLSSSTLALSY
                     HNRFVVKELSSISARYSMPTPWMNVGFLYRFFGFSLYHEMIAGVSVAKALSSHFSLGA
                     QVDYVSVYVSPEEGYLSAFVPQLGIIIKATPSVNLGIHAYNFTFANLQTSYQEEYLQP
                     YLTAGLDYHMDQKVLLLAQIHKPLREESTLSAGIEYTIAPEVIVRTGMSLQQYLQPSF
                     GVGLNIDVLQFDVGFQYHPVLGMSVSSGVVFIFGK"
     gene            23218..23817
                     /locus_tag="PLJ82_00310"
     CDS             23218..23817
                     /locus_tag="PLJ82_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOR39987.1"
                     /translation="MKNKLFLLGNIIVLLSLSLPTLATSYIQTAQGYILPGIVVGNDT
                     IPIVVLNEHIVYPKYVKKRDQQKYNKLVRDIKKVYPFAKVVGKEVERVNPLLEKLPQK
                     ERKKYMREYEKALFKQYEPDLKKLTLTQGKLLIKLIDRECEQSSFDLIREYRGGLTAF
                     FWQGFARILGANLKEDFNDAKSEDAMINRIIILIENGQL"
     gene            23892..24302
                     /locus_tag="PLJ82_00315"
     CDS             23892..24302
                     /locus_tag="PLJ82_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOR39988.1"
                     /translation="MLLIILFICLGIVIYLLNKYTRRETPAKIADTLTPIIEDTEVCC
                     GAHEVCEKDSLLASSPNAVYFEDEDLDIFRFRDEKSYNAKEIALFEEVFFTLREDEIA
                     EWLKSLQIRNINLPYAIKEEALLIVSERRYAHTH"
     gene            24330..25493
                     /locus_tag="PLJ82_00320"
                     /pseudo
     CDS             24330..25493
                     /locus_tag="PLJ82_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
     gene            25710..27239
                     /locus_tag="PLJ82_00325"
     CDS             25710..27239
                     /locus_tag="PLJ82_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR39989.1"
                     /translation="MNKRSIILLSVFMAITFFGLLFLQIKYLNSLLDMRVQHFNEAAK
                     RSLYQLSKTLEEEETLHYFNEALAQRPSQRSTSFIDQTVGLQSASSITIQENLQGSTT
                     TIQQKISISSSPSTTRTVQQISETLQNRLKENFAQKKILLDDIAMRWLKESTDKPIEK
                     RIDFTHMENQLKKELENNGITTPFKVILIDYTGEEVYRTSNFNVPNMEHYFSQLLFPN
                     DFNPKLNILRVYFPYAKEYLTQPVSFMIFSSVLFTIILFLVFLATIILIFRQKRLGEM
                     KTDFMNNMTHEFKTPISSISLAAQMLKDEGITKTPEVLKSISNIIYEETKRLSFQVEK
                     VLETSILEKEKSIMKFKEANVHEIIENVCNNFSIKISSKGGIINTNLQASEYWAMVDE
                     THFTNVIYNLLDNALKYRNLDQSPILKIKSWNEKDILYISVEDNGIGIKKEHLKKIFE
                     KFHRVPTGNVHNVKGFGLGLAYVKKIIEKHKGSIRAESELHKGTKFIITIPSLKKTQY
                     E"
     gene            27232..27936
                     /locus_tag="PLJ82_00330"
     CDS             27232..27936
                     /locus_tag="PLJ82_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR39990.1"
                     /translation="MNEQKVKILLCEDDENLGMLLREYLQAKGYDTDLLPDGDAGYKA
                     FMKNTYDVCVFDVMMPKKDGFTLAQEVRAVNTEVPILFLTAKTLKEDILEGFKIGGDD
                     YITKPFSMEELLFRIEAILRRIKGKKLKEKIVFKIGKFVFDSQKQILSIGDKHTKLTT
                     KESELLGLLCANANDILERNYTLKTIWIDDNYFNARSMDVYITKLRKLLKEDESVQII
                     NIHGKGYKLIVPQGVE"
     gene            28054..30027
                     /locus_tag="PLJ82_00335"
     CDS             28054..30027
                     /locus_tag="PLJ82_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR39991.1"
                     /translation="MQFKFSILFLLILVTLSSCSIQARINKADKKYELGEYYVAGNLY
                     KRIYPSIPSKEKELRASVAFKSGNAFRQINDNKRAETAYRNAIRYKYKEPLVYFYAAE
                     VARKNNKHTDAIKFYETFLESNPSHREALNGLSSSKLVLSDWKKPTRYVVSKEELFNS
                     RFSDFSPSFANSRGDLVFFNSTRKLNKKNKASRITGQNNNNIFSSRKNIQGKWEEPLL
                     VEGDINSEFDEGTCSFSFDGQELFFTRSKIEKGKTLGTGIFVSKRSGGSWTSPQQIIL
                     LQDSSLNIAHPALSPNGDYLFFVSDMSGGYGGKDIWKVERTKDGWGIPKNLGETINTA
                     GDEMFPSFKKNGTLYFSSDGHPGFGGLDIFKAEPSENNEWMISNMMSPVNSEADDFGI
                     TFMEKEEKGFFSSNRADRRFFDNIWSFELPKLEYKIEGKVTDNNGDILGDAVIRLVGS
                     NGTIEKISVRRNGTFSQIIAPNANYILLASARGFLNSSQKVSTENSNKNQTFTLNFQL
                     NSISRPVQMNNIFFKFGSAELTPESAVALNELVNLLKDNPTVTIEIAAHTDMIGTETA
                     NTLLSTQRAQSVVDYVKQKGIDTARLIPKGYGKNKPVEVDKTLAKKYTFLSEGVILDE
                     EFITSLPSQQQEIANQINRRTEFKVLKTTFQAY"
     gene            30062..30856
                     /locus_tag="PLJ82_00340"
     CDS             30062..30856
                     /locus_tag="PLJ82_00340"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOR39992.1"
                     /translation="MKQYHELLQRVLDEGTQKEDRTGTGTISVFGHQMRFKMEDGFPC
                     LTTKKLHLKSIIHELLWFLNGDTNVRYLQENGVRIWNEWADEKGDLGHIYGYQWRSWP
                     TYDGGFIDQISEAVHTIKNSPDSRRIIVSAWNVADLPNMNLPPCHAFFQFYVANGKLS
                     LQLYQRSADIFLGVPFNIASYALLLQMMAQVTGLQAGDFIHTLGDAHIYTNHLEQVQL
                     QLTREPRQLPTMKINPTITSIFDFQFSDFELVNYNPYPHIPGAVAV"
     gene            30869..31390
                     /locus_tag="PLJ82_00345"
     CDS             30869..31390
                     /locus_tag="PLJ82_00345"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOR39993.1"
                     /translation="MTNIAIIVAIDKQNGIGKNNQLLCHLPNDLKHFKQLTSGHTIIM
                     GRKTFESLPNGALPNRKNIVITRNQHVTYKDCEIAYSLDDAISKNAEKNIVFIIGGAS
                     IYEQAISVATMLYITKIDATFDADTFFPHINKNDWTLVKKESHQKDDKNPFDYTFETW
                     VRKTSSSQDILGI"
     gene            31520..33040
                     /gene="purH"
                     /locus_tag="PLJ82_00350"
     CDS             31520..33040
                     /gene="purH"
                     /locus_tag="PLJ82_00350"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOR39994.1"
                     /translation="MEKRKIKRALVSVFHKENLDKLILKLHAEGVEFISTGGTKSFIE
                     SLEVPCTAVEDLTGYPSILGGRVKTLHPKVFGGILSRRKLEGDMNQLAEYDIPEIDLV
                     IVDLYPFEATVASGASEQEIIEKIDIGGISLIRAAAKNFADVIIVASQAQYAPLYDLL
                     EENGAESSIEQRKYFAKEAFAVSSYYDTAIFNYFDGYENSAFRHAENTMKALRYGENP
                     HQRGFFYGKFNDMFEQLQGKEISYNNLLDIDAAVSLINDFDEITFAILKHNNACGIAS
                     RSNLLDAWKDALAGDPVSAFGGVLITNAIIDQTTAEEINKLFFEVIIAPDYDMEALEI
                     LSQKKNRIILIQKETKSKTTQFRSLLNGVLVQDKDLSIETEKDFKVVTQIEPSSSETE
                     DLIFANKIVKHTKSNAIVLAKNKQLCASGVGQTSRVDALRQAIEKANSFEMSLKGAVM
                     ASDAFFPFPDCVEIASQAGITSVIQPGGSIKDDLSIEFCDNNNMSMVTTGIRHFKH"
     gene            33062..34081
                     /locus_tag="PLJ82_00355"
     CDS             33062..34081
                     /locus_tag="PLJ82_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOR39995.1"
                     /translation="MGLFSFTQEIAFDLGTANTIIIQNGKIVLDEPSIVALDKRTDKL
                     IAIGEKARQMQGKEHESIRTVRPLRDGVIADFYAAEIMIRGMIKMISNKNSFFAPSLK
                     MVVCIPSGSTEVEIRAVRDSSEHAGGREVYMIYEPMAAAMGIGLDVEAPEGNMIVDIG
                     GGSTEIAVISLGGLVCNRSIRIAGDDLTSDIVDYMGRQHNIRIGERTAELIKINVGSA
                     LTDLEDAPEDYIVRGPNRLSALPMEVPVSYQEIAHCLDKSISKIEIAVLGALEQTPPE
                     LYADIVRNGIYLAGGGALLKGLDKRLQDKINIPFHIAEDPLRAVARGTGIALKNVDNY
                     SFLLR"
     gene            34206..35033
                     /gene="mreC"
                     /locus_tag="PLJ82_00360"
     CDS             34206..35033
                     /gene="mreC"
                     /locus_tag="PLJ82_00360"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOR39996.1"
                     /translation="MENLLRFLAKASSFLLFVFLQTVALLLLVSHNNYQQSIALSSAN
                     TITGNMYATIQSFTEYFHLQTTNMLLAEENTALQNENNILHQQLMKQNLSSINQDTVA
                     LFTYISAKVINSSVNKQQNYITLNKGSKDGIKPDMGVINSQGVVGIVSAVSKNFAVVM
                     PIINPRIKISCRIAKNDYYGSLLWEGRSPFFANLKEIPEHVELETGDSIITSGFSSIF
                     PEGIPVGTVEEVNTSKYDNFHDIKIRLNVNFQTITYVNVINFNQKTELNNLETQQLQ"
     gene            35030..35530
                     /gene="mreD"
                     /locus_tag="PLJ82_00365"
     CDS             35030..35530
                     /gene="mreD"
                     /locus_tag="PLJ82_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOR39997.1"
                     /translation="MILTIARNTLFFVVLVLAQVLLFNNINFLGFINPYVYVLFILGL
                     PVALNKKSIMVVAFCLGLLIDIFTNTLGIHIFASVLIAYLRNFWIDALFFRNDFDFVS
                     PSIKNFGFFNFVKYAFVLIFVHHLALFILEVLTFNHFLLMLLRVLYNSIVSLLLIISF
                     ELIKSK"
     gene            35527..37398
                     /gene="mrdA"
                     /locus_tag="PLJ82_00370"
     CDS             35527..37398
                     /gene="mrdA"
                     /locus_tag="PLJ82_00370"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOR39998.1"
                     /translation="MKDSIKNQELRNRQYIIILIVLSVAFIYIIQLFYIQIADSSYKE
                     NADSNAFLKKVIYPSRGLLYDRNEQLYVSNQPVYDLMIIMREIQGFDTLSFCKMLNIN
                     QEEFNNRIEEIKDKRKNRGYSRFTPQIFMSQLSVAEYGKIQEQLYKFRGVYAQQRVLR
                     KYTTPHGAHAIGSVGEVNQQQIDADSYYSRGDYKGQSGVEKEYETYLRGEKGVEILLR
                     DAHGRIQGVYKNGERNKAPIAGKNLTMAIDMGLQEYGEQLMIKKLGSIVAIEPSTGEI
                     LALISSPSYDPHLLVGRERSKNYNALLKDPFKPLFDRPLMAMYPPGSTFKLVNGLIYQ
                     QEGVITEDTRFSCSMGYYYSRTRKLGCHPHPNNLDLRESVQHSCNAYYCHGLKGLLDS
                     PPSGISTTESYNHWRNHVLSMGFGKKLGVDFPNEKGGLILESNRYDRIYGKNKWKSST
                     IISIAIGQGEVLATPIQTANLAALIANRGYFYTPHIIKKIEGDSIDKRFREKHQTTIE
                     QKYFDPIIDGMLMAVEQGTGRVGRIEGIEICGKTGTAQNPHGKDHSIFIAFAPKDDPK
                     IAVAVYVENAGFGATWSAPIASFVIEKYLNDSINKSRIWLENRILEGDLINVQKTNN"
     gene            37376..>38110
                     /gene="rodA"
                     /locus_tag="PLJ82_00375"
     CDS             37376..>38110
                     /gene="rodA"
                     /locus_tag="PLJ82_00375"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOR39999.1"
                     /translation="MFRRQITNIWNSLDWFTVALYAILVIMGWFNIYAAVYNEEFGSI
                     FDISQKYGRQLLWILAAIVLIVVIFLIDNKIYVYFSYPIYIAVMLLLISVLLFGVEIS
                     GSRSWFQIGEFKIQPAEFSKFATILAIANFLSEPGRRLMKLPNFLVVLTLIAIPTILI
                     LLQNDTGSALVFTSLVFMLYREGLPGWILLIAFFAIVLFIIALTKPFYWVVGLVIIAC
                     WIYAYLKYNAKRDFIYFGIFMLMIIGA"
BASE COUNT        13350 a         6769 c         6571 g        11420 t
ORIGIN      
        1 ttataatgag aaatttcctc aaattatatg gttttttatt gttttttgtt cttggtatca
       61 ttgggctaaa cgctcaaagt gtgtccatta atgagataat gtcgtctaat tcaaaaacaa
      121 ttaccgatga agatggcagt tttgaagatt ggattgaact gtacaactat ggagatacac
      181 ctgtcaatct acatcaattt ggcttatctg ataacgcaaa caaccgattt aaatggacat
      241 tccctgaaat tatgctacct ccacaggaat ttctactagt ttttgcatca gataaagaca
      301 gaaaatttgt cccaatggaa gagcaagtac ttgtttcgtc aaccactata tggaagtatg
      361 tagacgctgg cgaagattta ggttctgatt ggaaagaaat tgattttgat gataccgaat
      421 ggaaaacagg aacagctcct ttaggatata atacaaatgg aaatgaacgt tatgctacag
      481 ttttagatta tgggagtgat aaaagcaaca aatatataag ctatcaggtt agaacattat
      541 ttgaaaaacc tacagcagat tttcgacagt ttactataca acttgtggcg gatgatggta
      601 ttattgtgta tctaaatgga aaggaaataa tccgtcagaa catgcccgat ggagaaattg
      661 cttatactac cttagctcaa agtgggatca ctagcattga cacaatcaat taccaactag
      721 atggttcgct gctacaaaat ggaacaaata caattgccgt ttctgtacac caagttacct
      781 tgaatagtag tgatatttac tttgacatga aattgtggac aaacaaacaa tatccacatg
      841 ccaattttaa aatagcatct gaaggagaga ccctttttct gacaaacaaa gaaaattcaa
      901 ttatttcgaa cgtagcatca gtatatattc cttcggatat ttcgtatgga agaacaatag
      961 atggcaatgg tgatttcgcc ttttttgcaa cacctacgcc tggcactagc aacatcacag
     1021 agagtagctc tgaagtactt gtaccagtaa atttttctca caatggaggc gtatacacag
     1081 aagaattatc attaacactg tcacatccag accccgaagc aacaattata tataccattg
     1141 atggctctat cccttctatc aacaatttag gaggtactac atatctatac aaaaatgaat
     1201 tcccaaattt aagagaatct cctgaagcag attcgttatt taggtctttt caaagctatg
     1261 agtatacagg agaagaaatt ctagtttatg ataaaagtgc tgaagacaat caattatcaa
     1321 atatcacaac ctccgttatc cacacatatc ttcctgataa ccctataaga aaaggttttg
     1381 tcgtacaagc aaaagcatat aaaaatggag ccattagttc acatatatca ggacagactt
     1441 attttatttg ggaagaaggg aatccgtata atgttccgat tatctctttg caaatccaag
     1501 aagacttttt gttcgattac aataaagggc tgtatacacc tggtgccgat tgggataatg
     1561 cattagcatt agacgaagat gcaaatcctt gtttatttgg taattatatc agcacgcgtg
     1621 aatatcctgc tcatgtggaa atattcgatc ctttatctcc tgaaagtgat gttaatcaag
     1681 gtgttggtgt tagaatacac ggtggatgta gtaggctaac tcctattaaa tcattaagac
     1741 tttatgctcg taacgaatat gatagaatgg gggaaataaa ctataaccca ttttccagta
     1801 taccctatca agctagcaac ccatcgaata ccctattcaa gcggatgtta ttacgttttt
     1861 caaccagtgg aggacctcaa atagttgata ttgttaccca taaaattatg gaatccgtgt
     1921 atcctggtgt acaacgaaca cgccatgcta tcgaatttat aaatggagag ttttggggta
     1981 ttactgctat acgagatagg ttcgaccaat accatttagc gtataattat aatttagatt
     2041 cagataatat aattttttac acaccatggg gtattgtaga tgaagggctt cctgaggata
     2101 aagagctatt tactagtatg tataattata tttctactgc agatttaagt acagaaattg
     2161 ccaaagcaaa ggtcgaatca ctacttgata ttcaagatta ttttgattat tacatcatga
     2221 atatatattt tggcaaagtg gattggtatg gggatagaca ttttgcattt tggcgtgtac
     2281 gcacacccgt aaatagtcaa tttggagatg gtagatttcg tacatttact tgggattttg
     2341 atacagctac aagtttgggg gctgagtaca atatgctaca cgaagcccta catgaatatc
     2401 atcctcaaca cattgctatg ataaataatt tactgacaaa cgagcagttt aagtatgatt
     2461 ttatcaatcg ttttgcagat catttaaata ccacattttt gccagaaagg gttgagaaca
     2521 tagtgcatca agaagcatcc gaatttagct catatttacc tgaacataac gatagatggc
     2581 caaatatata ccctggtcca gaagatatta atgatagatt gattcaatat gcggttgata
     2641 aatctacgat acaacgcacg caaataatgg acgaatttgg gttatctgga acttatgccc
     2701 tgaccattga tgtatcagat aaaaccgctg gaagtgttcg tattaattct attgacatac
     2761 aatcgacaac acctggtgtt tcggacaatc cttatccatg gtctggagtc tattttagtg
     2821 gtatccccgt taaacttact gcaatcccaa aagaaggtta tcgtttctcc cattggtcag
     2881 gggtgataaa tagtactagt aatagtattt tagttgatat aacaaatgat gattctatac
     2941 aagcaaactt tgtacccatt ggaaatgatg gcgatttaat ctatttttgg ttgataaata
     3001 acactattgc aaatgataca ccattatcgg agattcctgc tacattctca aaaatgccag
     3061 aagaatatgc atctctcgaa ttttcgtctt gtttaattgg ttatcctttt aacagcactc
     3121 atagtttatg gagaaaagct tctatggaaa gaaagaacgc accaacatcc ataaattact
     3181 tccccgaagc aaacaataat cgtacatatg gcacttttac tatgagggct ttacaaataa
     3241 aagaaccttt tcaacaaaat gacataaaaa atacactcta tctcaatatt cctactacca
     3301 gctataagga tatcattgta tcttttgcag caaaaaatga aggagcggca aatggttttg
     3361 aagtagatta ttgggatatc aactctggca aatgggaaca aacagatatg aatgtttcaa
     3421 cttttaattt atatagtgaa tatcagcgtt acgaaatctt attcaaaaat gtactaacag
     3481 caaatgacaa tcctgatttt cgtttacgga taggttttac aggagctact ccatctacag
     3541 aagataacgg aaataatgtt gtatataata attttgcagt acaaggtact ccattactta
     3601 taacacgcat tcttgaatta gacaactcag ctattttaac aaccattttc ccaaatccag
     3661 cgagtaatta tgtaaaagtg tattgtagtt taccaatcat caagagcact ctaatcaaca
     3721 cattgggtat tactattcct acacacacaa ttacatctac aagcactgaa ataaccttag
     3781 atataagtca gttgtcttta ggagggtaca ttttattagt tgaaactgcc gaaggaatat
     3841 cgcctgtgaa atttattaaa cgataatcta tcacatttct ttgcgtaaac gtgccacagg
     3901 aatatccaat tgttcgcgat attttgctac agttctacga gcaatattat atcctttttg
     3961 ttgtaatatt tcagttaatt tatcatcaga caagggttta gatttatctt cagacaaaat
     4021 tgtttggctc aaaatattct taatttctct tgtagaaact tcttctcctg attgagtaat
     4081 catcccctca gtaaaaaaat ggcgtaatgg aaaaatgcca aaatttgttt gaatgtattt
     4141 actatttgct acacgtgata tggtagatat atcataacca gagagctcag caatatcttt
     4201 catcgtcata ggacgaagaa aagaatcact tccttcaaga aaatactttt tttggtaatt
     4261 tacaatcgtc gaagttactt tgagcaatgt ttcctctctt tgttttactg catctataaa
     4321 ggatttggct gcatctaatt tttgttttgc aaacaaaact gcatctttca tagattgtgt
     4381 ttgatttacc ttgttagata caaaatcttg gaataaattt acatacgttt gattgacccg
     4441 tagcatagga atagtactat tattcaatga aagggttaat tgattatctt gtacctcaag
     4501 aataaaatct ggcgtaattt cctccttatt tttttctaat atcccttccc atccatttcc
     4561 tggctttgga tttagatgta taatttcatc aatagcatcc cgtaaggatt cattatcaat
     4621 agccaattta gcactaatct tttcataatg tttactacta aattctataa aacaatcttc
     4681 caaaatttgc atagcaagat caattgatgg tgtcaactgc tttcgttgca attgcaataa
     4741 caaagattct tgtaacgaac gagcccccac tccagcaggt tctaattgct gcacaatagc
     4801 caatacttcg tataactgag agtctgtata gtgaacacca gtcaatacac ttatatctgt
     4861 agctatctgc acaacttctc tacgtaaata tccttcatca tcaatattac caacaatata
     4921 ttcggctaac aataattgag tttttgataa gggtaataaa tgtaattggt ttaataaata
     4981 ttcatgaaat gattctcctt ctgaatatgg aatattatat acactagcag gcatattatt
     5041 agggtcttgg ggcatgtaat caccatcttc atcatcttgc atatagtcat ttatcccaaa
     5101 tgtatcatcc tcatcatctt ctacatcttc ttcatttata gagttatctt cttcaggagc
     5161 tccttcttcc aatgccggat tttcctctat ttcagtttta attttttctt ccagttctac
     5221 cacagaaaac tccaacaact tgatgatttg aagttgcaaa ggagataatt tttgttgaag
     5281 cttttgctga aactgttgct ttaatgccat gactattttg ttaaaacaat acaaatgtac
     5341 attatttttt ttaacagtat aaacaaatat caaaaattta cgtaattttg ctagaacatt
     5401 caaacacaca aacttgtata acactttcct ttctcaatgt gaaataacat gaaaacaatt
     5461 ggaaaaatct tttggggatt ctatttaatt gtattttttt tacctttggc aatacttgct
     5521 acaatgctta ctgcacttac cactattgtt atgtgtggca ttggtaatca tcgcatttgg
     5581 ggatatatac caggtaaata ttggtcaaaa ataatgtgtg ccttagcttt tgttaccgta
     5641 gaagttaaag gtaacgaata tcttaaccct aatcagtcat atattttcgc agctaatcat
     5701 caaagcttat atgatgtatt tttaatatac gggtggattg gtttttcttt taaatggata
     5761 atgaaaaaag aacttcgcaa aatacctttt gtaggatctg catgcaaagc agctggacat
     5821 atttttatag acagagaaaa tcccatcaaa gcacttcaca gcattgaaaa tgcaaaaaaa
     5881 atactcactc aaggtgtttc gttggcaata tttccagaag ggtcgcgtac gcgtactggc
     5941 aatattggta aatttaaacg aggagccttt caaatagcaa aagacgtaca gcttcccatt
     6001 gtgccagtta caatagatgg aggatataca atcatgccat tttcacgtat ataccctgtg
     6061 ccgggcaaac taacacttac tattcaccct ccagtatcat tttctccagc atctcacgat
     6121 gaagaactaa aaatgattga taatataaga aataccatca taacgggctt atcatgaaaa
     6181 aacttttttg gatgtatatc atagtgtatt tcttactcat tccggctgtt ttaatggcta
     6241 gagggacaga acgcaccaat caattacacc gagaagctca gttagcttta accaaaggag
     6301 acattaacga aggaatagca atctatgaaa agataattaa acaaaataaa aaccaggctg
     6361 aatactattg gttacttgat gatgtattaa agcaactatt acaaaacaac aaacgcattg
     6421 aattgttaca aaaactaagt aacaataaaa aggcaaattc tcccgaacaa accaaagaaa
     6481 aattaggatt ggcttatttt gatgcgggta attatgacag tgcaatgctt atctttaacc
     6541 aactaaaaca aactagagcc atcaaacgtt atatctccaa ctgtgaaaaa gccaaagtaa
     6601 tccgcaacaa tcctattgac ataacgatta cttcgatggg agaaaatatc aatacaatgt
     6661 atgataatat ttggccagga attaccgctg ataatgagac tttttcattt accgtagttg
     6721 aaggtaaaac aaaccctttt accaatcctt atgcgattca agaagatatt tatcaaagca
     6781 ctaagcaaga cgaatgttgg caaaaaggga aacggttatc tgctccaata aacactactg
     6841 acaatgaagg ctctcaacat ttttcagcag acggaagata tatgtttttt gtgggatgta
     6901 accgtaaaga agggaaaggg agttgtgata tctactattc aatcaagaat ggagacagct
     6961 attctgcacc tattaaccct ggtgaacctt taaatacaat atattgggaa agcaccccta
     7021 gtttttcagc ctgtggcaaa tttctgtatt tttcgagtaa tagacctgga agcattggag
     7081 gcaaagatat atggagatgt gaaataaaca tcctaccttc gggtcaactc acattcttca
     7141 atgctcaaaa tatgggagat ccaatcaata cttctttcga tgaaatggct ccttttattc
     7201 atccagacaa tattcacctc tatttttcat ctaacgggca cccagggcta ggaggatttg
     7261 atgtatttca agctaaaaaa gacacactag aagcttggaa tactatcagt aatatgggtt
     7321 atcctattaa tacgtttggc gatgaaatag gatttatcat ctcctcttca ggagaaaaag
     7381 catatttatc atcaaatcaa catattgaca ttgataatca acatctaaat agaatgattt
     7441 atgaaataac tttgcctcat tcgttgcgac cagaaacaat gcaaacagta aaaggagttg
     7501 ttattgacaa taaaacaaac caacccttgc aagccaatat cgaaatcttt gatactcatt
     7561 cgcaaaaaac aatttcacga actacgtctg acaataaaac aggcaccttt acagccatta
     7621 ttccccaaaa gagtcaagtg ggaatgtttg taaaaaaaga ccaatacttg gtgtattccg
     7681 aacaaattga ttctacttct tcagactttt atacaataaa acttgaaaag attgaaaaag
     7741 ggagaagctt tgttcttcgc aatatttatt tcgattttga ctcagccgaa ctactctcga
     7801 attcgtatgc tgaattacat cgattgcttg agtttcttca atcaaaccct accttaaaca
     7861 ttacattaat tggtcataca gacaatttcg ggaacaagga gtataatcga agtttatcta
     7921 tacaacgtgc ccgttcagta tataattatc tactaaaaaa agggatagat aagtcgcgat
     7981 tacaatttga aggcaaagga tccgatatgc ctattgaaga caataacaca gattttggta
     8041 ggagtagaaa cagaagaatt gaatttgtga tacactaatt ttattcacat gtaacatacg
     8101 gcttaatttt ttcgatgaaa tcgctattta tatcccgatt aaatggaatt tcttcgatgt
     8161 tttctatccg tttagttcgc ttacccatcc tatactcata aatgctttgt gcttgataaa
     8221 acgatatata cggatgttgt tttagttgat ggacagtcgc ttgattaata gaaatcttct
     8281 tagtataaga ctgtgataca gttagttgcg gagcaatttt ctggtatact tcatctgtta
     8341 tcccatatac ctcttttagt tgctcaacag aaataaatcc gccaagtttt tctctatacg
     8401 taacgattcg ttttgataat acagagccaa taccctttac tgttttcagc attgttgtat
     8461 cagctttatt gatatctacc aatgttattt cactcgttgt tacaggtaat gattcctttg
     8521 attctactag ttgtttgtcg cgtgcgatgg gtttaatttg aatatatggc aacaatcgtt
     8581 catacatttc ttttggcaac ccgtagattt tagaaaagtc agtaggcgat cgaaataccc
     8641 cacctttagc tctatacttt aaaatacgtg atatcacata gggttttatg cccagtttca
     8701 ggaataaagc tgaatcagca tggtttgggt caaatacttg cagtatactc gtcgtagaat
     8761 caatattttt gctccaatgt tgttttttat atacaaatga agctgaatct tcttgtaatg
     8821 ttttttcaaa atctgctatt tgtaacttca cttcctcatc atccagaacc acaggattag
     8881 agatgaaata tggtaaacca acatatgtac caattaatac aataagcatc actatcaata
     8941 cagacaaccc tcgcttttct tgtttcgtaa aataaaaata atctttccaa gacatgttta
     9001 cgcgaataga ttatgaacaa aaacaagcat aatacataca ggagctaagt agcgtaacaa
     9061 aaagatatac gttttaaaaa agcggaattt ccaagtcccc tcttgttgta atacatccaa
     9121 cagaatacgc ttatctacat accagccaac aaataatgac acggcaaccc ctcctaatgg
     9181 caacaaaata gtagccgtaa taaaatctaa ccattcgaag agcgttttat caaaaataac
     9241 gggaaataat aaggttaaag ataacgaagc taaaacagct aataccaaca ctgctacaga
     9301 tacaagcgta acagcctttt ttctagtata ggaaaattct tctttcacaa aagccgtaac
     9361 aacctcgtgc aacgaaataa ccgaagtaaa agcggctagt gctaacaaca aataaaatag
     9421 acttgaccaa aaaataggaa aaggcatttg ttgaaaaatg gtaggtaata taacaaaaac
     9481 tagtcctgta ccttcatcgg gctgtaaacc aaacgaaaat acagcaggaa aaattactac
     9541 accagccaca attgcgacta aagaatcgag caaagtaacc tgaaatgcaa tgcgaggaat
     9601 atgtgtatct ttaccaaaat atgaggcata cgtaattaaa caacccatac ccaaactcaa
     9661 cgaaaaaaaa gcttgtccta atgccgttaa tatcccatac gaagtaacat tttcaacttt
     9721 tggctcaaac ataaatcgta ttccctgcat tgcattaggc aacattaacg aatgaatggc
     9781 taaaataatc atcaacacaa ataacatagg catcaatact tttgatactt tttcaatccc
     9841 atttttaacc ccatttatta ttacaatatg tactaaagca atcataatta cagtccaaat
     9901 aacaggctta ataggacttg ctataaaaga atcaaacatc actttgtact cttcggacga
     9961 tttatgtaac aaattccctt gaattgcttg ccaaaaatat tcagcagtcc aaccagtaac
    10021 tacgtaataa aaccccataa tcataaaacc tgccacaacg cccataatac caactaaaaa
    10081 ccatttcgta ttaggagcta gtaatttaaa cgaacctacc acattggctt ttgctttcac
    10141 tcccacagca aattctgcca ttaccaacgg aaatcctaag aaaaaaacgc acagcaaata
    10201 aattaaaata aatgcacctc ctccattttg accagcctca tacgggaaac gccaaatatt
    10261 tcccaaccca actgctgaac caacagtagc aagcacaatg ccaatttttc ctgtaaaatt
    10321 atcccttgtt tccatcatgt atctatatta tgactgcaaa aatacataat atttacaaaa
    10381 aaaatgtata aacgattgca tattcatttt ttatgtatat ttttgcagaa taattgtata
    10441 tttatttaaa aatgaaagaa cgtaacaaac ggctagaagc tataaaagaa attatagcac
    10501 atacgacaat tagcagtcag gataacctcc ttgaatcgtt gattgataaa ggttttaatg
    10561 caactcaagc tacgttatcc cgcgatttaa aacaactcaa aattaccaag atattaaccg
    10621 atgatggcat ttataaatat gtaatcccat ttacaaatta tgcaaataaa acaattggaa
    10681 atacgcgaga gattatctct attgattttt ctggacaatt agcagtaatt aaaacgagag
    10741 caggctatgc caatggtatt gcatctgata tagatcaaca acttgctgaa ttaatacttg
    10801 gaaccattgc tggagatgat acaatattat taatccctaa agaaggtatt actcgcaata
    10861 ccatcactac agcaatacgc tcatttatga cagttatttc ataactaatt atataactta
    10921 tttgtatgga atctgacact ataaaaataa tggttgctaa cgaaagccac tatacataca
    10981 ttgacgaaat tctttctacc attgaagagt cgtcaaaaga ccgtggcaca ggtattgcca
    11041 aacgatcgcc cgaatatgta aaaaataaga tggcagaagg caaagcaatt atcgcacttg
    11101 atggcgaggt atttgctggt ttttgttaca tcgaatcgtg gggaaacaag caatatgtag
    11161 caaactcggg attaatcgta aaccctaaat atagagggca cggcgttgca aaaaagatta
    11221 aaaaattctc ctttgattta tctcgccaac gttttccaaa cgctaagtta tttgggttaa
    11281 cgtcgggagc agctgtaatg aaaatcaata cagaattagg atacgtaccc gttacctttg
    11341 ccgaccttac cgatgatgaa gaattttgga aaggatgcca aggatgtgtt aactttgacg
    11401 tattacaacg caccaacaga cgctattgta tttgtacagc tatgttgtac gacccaaacg
    11461 atcctaatcg tagaaacaga gaagaaaaag aaaacgaagc aaaaaaacaa aacaaaaaaa
    11521 gcgttattac cgactttatc aataaattta aaaaattaaa ataaattacc cgtatcatga
    11581 aaaaagaaaa agtattatta gcatttagtg gtggactcga cacttctttt tgtgccatgt
    11641 atttagccaa agataaaggt tacgaagtgc atactgcaat tgctaataca ggtggattta
    11701 gccccgaaga gttaaaacgt attgaagaaa gagcctacag actaggagcc aaaacacatg
    11761 caacccttga tgtaacgcaa gaatactacg ataaaagtat taaatacatg gtgtttggca
    11821 atgtgttacg caatggcaca tacccaatct ctgtaagttc agagcgtatt tttcaggcaa
    11881 ttgccaccat cgaatatgca aaaaaaattg aggcagatta catcgctcat ggatctacag
    11941 gtgcgggaaa cgaccaagtg cgttttgatt taacgttcga cgtattagca ggtcatatta
    12001 aggtattaac gccaacacgt gatatggtat taacacgcga atacgaaatt aattacctaa
    12061 aagaacatgg ctacgaagct gattttacca aaatggaata ttccatcaac gcaggtttgt
    12121 ggggaacatc tattggtggc aaagaaacat taaaatcgga acaaacattg ccagaatcgg
    12181 catataccaa gcaagtagta aaaaaagaag aagaaactat tacaattgac tttgaaaaag
    12241 gagagattgt aggcgtaaat aatgtacact ataccgacaa aattgcagct atcaataagg
    12301 tagaagaagt aggttcagct ttcggaattg gtagagatat gcatattggc gacactatta
    12361 ttggtataaa agggcgtgtt ggtttcgaag cagcaggggc attgcttatt attaatgctc
    12421 acaaaatgct cgaaaaacac actcttacca aatggcaaca atattggaaa gaccaattag
    12481 gaaattggta cggaatgttt ttacacgaag ctcagtatct agaacccgta atgcgtgata
    12541 ttgaaaaatt cttagaaaat tcgcaacaaa acgttaccgg aaaagtcatc atcaaactgc
    12601 gaccctattc gtatacctta gtaggtgtag atagcccgtt tgatttgatg aaaaccgatt
    12661 ttggcgaata tggcgaagta aacagagctt ggtcggctga cgatgtaaaa gggtttacca
    12721 aaatactcgg aaaccaaatg aaaatatatt ataacgttca ggctaaaaac aacaaataaa
    12781 gatgataaaa attggaatta taggaggtgc tggttataca gcgggcgaat tactccgttt
    12841 gttggtacat cacccacaag cagatatagt atttgtaaat agcaccagta atgctggaaa
    12901 taaattatat gcagttcata gcggattgat tggcgaaaca gaattggaat ttaccaacga
    12961 actaccgctg gatgcgattg atgctttgtt tatctgttca gctcatggtg atagtcgtaa
    13021 atttttagat gcgaatacaa ttccttctaa ggtaaaaatt attgatttat ctaccgattt
    13081 tcgcgacgag agcaatggtt ttgtatacgg actaccagaa ttaaacaaac aacgtattct
    13141 gcaagcgaat aaaatcgcta atccaggatg ctttgcaacg gtcattgaat tggctctgtt
    13201 gccattggca gccaacggat tgcttaccaa cgaagtgcat ataaatgcta ttacagggtc
    13261 tacaggggca ggagtaaaac caagtagcac gtcgcatttt agttggagaa acaataatat
    13321 ttctgtttac aaagcatttg aacaccaaca cctaaaagaa attacgcaat ctattacaca
    13381 attgcaaccc tcttttggca aagcaattaa ttttattcct gtaaggggta attttgctcg
    13441 tggtattttt gcttctgtat acaccgattg cagcctatcg ctagaagaag cgaaagcctt
    13501 gtacaaaaac tattacaaag atgctgcgtt tacgtttgtt acagatacaa atcctgattt
    13561 aaaagaagta gtaaacacaa acaagtgtat cgtatatctt gaaaaacatg gcgataaatt
    13621 acttatttta tcgtgtggtg ataatttatt gaaaggggct tctggacaag cagttcaaaa
    13681 catgaatcta ctatttgggc ttgacgaaaa aatgggctta aatttgaaga atattgcatt
    13741 ttagttacaa aacgacattg ttcgttatct taaaaaatga aaaaatgaaa ttatttgatg
    13801 tatatccgct attccctatt gaaattgtaa aagggaaagg atgcaaagta tatgataaca
    13861 atggtactga atacctcgac ttatatggtg gacacgctgt tatttcggtt gggcatgcac
    13921 accctacgta tgtgcaagca gtagcagatc aagtaggaaa attagggttt tactccaatt
    13981 cggtaatcaa tggcttgcaa gaaacatttg ccgagaaatt aggcattgcg tgtggatacc
    14041 ctgattattc gctattttta atcaactcgg gtgcagaagc tactgaaaat gcactgaaat
    14101 tagcttcctt tcataatgga aaaaagaaag tattggcatt caaaaaagct tttcatggac
    14161 gtacctctgt agctgtaaaa gtaacggata gtccaagcta cgtggcacct atcaacactg
    14221 gtttagacgt aacattttta gctcttaacg acattgaagg tgtaaaaaaa gaacttgcaa
    14281 caggcgatta tacggctgtt atcatcgaag gcatacaagg tattggcggt attattgagc
    14341 caaatatcga ttttttgcaa gcattacgtt tggaatgtac acaaacaaat acggtactta
    14401 ttttggatga aattcaatca ggatacggtc gtagtggcaa gttttttgct catcaatatg
    14461 ctggcatcaa ggcagatatc attacggttg ccaaaggtat tgcaaatgga tttccttttg
    14521 ctggtgtgct tattagtcca atgtttaaag ccgttcacgg acaattaggc acaacttttg
    14581 gaggcaatca tttaggttgt gcggctgcat tggctgtgtt gcacattatc gaaaaagaga
    14641 atcttatcga aaatgcaaaa caaattggcg attacctact acacgaacta aaacaaatcc
    14701 ctcaaataaa agaagttaga ggcaaaggat tgatgattgg tatagatttt gaacaagctg
    14761 taaaggaaat tcgaaacaaa ttattagtag aacagcacgt atttacaggc gtagcgggca
    14821 cacatactat tcgtttactc cctccacttt gtttaacaaa agaggaagct actgaatttt
    14881 tgttaaaact aaaacaatgt ttgtaaaaat gttgtatatt tgtggtaatg tataacccat
    14941 aaaaaatgaa ccgttatgat tagtaaaaaa atgcaagatg cgattaacgc acaaataaat
    15001 gcagaatttt ggtctgcata cctttattta tctatgtcca ataattttgc tgcaaaagga
    15061 aatcctggtt ttgcaaactg gttcgaaata cagtttaaag aagaacaaga ccatgcaatg
    15121 atttttggca aacatatttt ggcacgtgga ggaaaagtat ctttggctcc aatcaaagaa
    15181 gtgaaaaccg aatgggcaac tccgttggaa gcttttgaag aaacccttaa acacgaaaaa
    15241 gtagttactg gcttaatcaa taatctatac aaaattgctg tagaagaaaa tgattacgct
    15301 acccaaagta tgctaaaatg gtttatcgac gagcaagtag aagaagaaga aacagctcaa
    15361 gagctaatcg atagcctaaa aatgattgcc aacaatggtt ttggtattta tatgcttgat
    15421 aaagaattaa gcactcgcac atacaaaaca ccaagtcctt tagtagcaaa agcataaaag
    15481 aaaattcgta aggtcgaaaa tggagtattc tttttcgacc ttatttttaa ctatcattta
    15541 ctggattgga attaataaca tactttttat gaaaaaaatc agcattattg gagcaggcaa
    15601 tatgggtgga gctattgcta gaggattatc tcaaggttct atcttttcag cccaacagat
    15661 tactgtaagc gatatttcaa ccgaaaactt acaggcaatt aaaaccttca accctcttat
    15721 taatacgact cttagtaatg tagatggggt gcaacatgct gatattgttg tgatagctgt
    15781 gaaaccttgg cttaccgaaa caatttgtaa cgaaattaaa tctgtattga actatgacaa
    15841 acaagttgtt gtgtcaattg ttgctggtgt ttcttttgag cagttacata catttttaaa
    15901 gaaagaagga gagtccagtc caaccatatt tagggttatt ccaaatacag ctattgatgt
    15961 attgcaaagc gtttcaacca tagcctcatc gcatgcaaac aaacaacaag aagaagaaat
    16021 tgtacgtata ttcaatgaac ttgggaaagc ttttttagtt agttccgaac aacaactcaa
    16081 cgcctttatg tcgctttcaa gttgtggtat tgcgtatgcc tttcggtata tacgtgccgc
    16141 tgtagaaggt ggtgtagaaa tgggcattta tccagaaaca gccaaacaag tagtgctaca
    16201 aacattgcgc ggagcaattg acctactcga agctaccaat gcacatcccg aagccgaaat
    16261 tgacaaagta accacacctg ctggtattac cattaaaggc ttaaacgaaa tggaagccaa
    16321 cggattctca aacgctgtta tcaaaggctt aaaagcatcg tatataaaat aaactgcctt
    16381 ataattcaat tttatcgggg taatcgagtg tgtaatgcaa ccccctactt tccttgcgtt
    16441 gcattgccat ttttattact aaataggcgg tattgatgat attacgaagt tcgcaaatat
    16501 ctttggttac cactgaacgg gtaaacaaat cttcggtttc tttgtacaaa atctctaatc
    16561 gatccatggc tcgttttaaa cgcaaattag accgtacaat acccacataa ttagacataa
    16621 tttgccccac ctctttggca ctttgtgtta tcaacaccat ttcttcgttg agcgaggtac
    16681 cttcatcatt ccattctggc acttgctcat taaaaacaac accttcaatc ctacctaacg
    16741 catgcgtagc agctttgtcg gcatacacta ccgcttcaat taaagaatta gatgccaaac
    16801 ggttagcacc gtgcaatcca gttcgcgaac attctccggc tgcgtacaaa ttgcgaatac
    16861 ttgactgcga atgtacatct acctctatac caccacacaa ataatgagct gcgggcacta
    16921 cgggtatata atctttggta atatccacac caagcgacaa acatttttga taaattgtag
    16981 ggaaatgtgc ttttgtctct tctgccgatt tatgtgtaac atctaaatag acaaaatcat
    17041 ctcccgaaag tttcatttca gagtcgatag cccgtgccac tatatcacgt ggagctagcg
    17101 agcctcgttc atcgtattta tgcataaact gacgcccttt gcgtgttcgc aatacagctc
    17161 cgtatcctcg cattgcctct gttattaaaa aagagggtct ttcatttgga ttataaaaag
    17221 atgttggatg aaactgtaca aattccatat cgctcaccag tccttttgct ctatacacca
    17281 tggctatacc atcgcctgta gcaatagatg gattggtcgt tgactgataa atactgccta
    17341 tacctccagt agccatcatt gtaacttttg cgagaaaagt atgtacatga cgcgtttttt
    17401 cgttcagcac atacgctcca taacattcga tattcggtgt agtgtgcttc accaacatgc
    17461 ccaaatggtg ttgggtaata atctcaacag caaaatgatt ttcaaaaaca tcaatgtgtg
    17521 ggtgcttttt tatttgtcgc accaatcgtt tctgaatttc agctcccgta gcatctttgt
    17581 ggtgcaaaat acgaaaatca gaatgacctc cttctttgtg caaatcaaaa tttccctcat
    17641 ctcctttatc aaaatcaaca ccccatttta cgagctcatc tatttgcgaa ggtgcatttt
    17701 caactaccat acgcaccacc tcttcgttac ataatccatc gccacaaatc aatgtatctt
    17761 gaatatgttt ctcaaaatta tccgaatctt tggtaacaga ggcaatgcct ccttgagcta
    17821 aattagtatt ggcttcttct aatcgtgttt tactcaaaat agccactttc cctttatggg
    17881 caaccttaag ggcaaaactc ataccagcaa taccactgcc aataacaata aaatcgtatt
    17941 ttcgtaccat attgaatagc taaaatgaaa gacaaaggta taaaaaatct atataaatct
    18001 attcaattac tacttcttat tcagagcata ttacttttgg taaaaaaatc ttcaatattt
    18061 tgcttttctc aacacattga attacatttg ttgccaaaca aatagctatc tatatgaacc
    18121 gaatctataa aattagctcc ttactcatct ttttgttcat catcaatctc atacagagct
    18181 gtggtcccag tatggatggg tacataggct ttgatattac gcacatacag attcaaaatc
    18241 tttccaatac tggctctgat gctgtggtta ctactgaaaa caccatgctt ccgagtgctg
    18301 ttgcttttaa agtgaggctt tacgaaggct ctaatagtgt tagattacgc atgcaaccac
    18361 aaacgaactt cggtttcagt tcgtgtgtag cccttagtcc gccagatttt tactacaaat
    18421 cgaaccaact cattcaaaat atccgagtag ttacgttggt ggattattcc acgtccattg
    18481 tagccaatac cgatgtgtcg gataaatttt tggtatatcc gatagtacca gctatatctg
    18541 attttttgta tgtgagcatt cccgaattct gcacgctaac aaccgaccaa atactgatgt
    18601 ctcctagtat tgaatttcat cttatgtgca aggaaacact ttctctcaac aacgctcaat
    18661 ttagaataat cgttgaatta tcgaatgggc aagagttgag tattgataca gcattacttg
    18721 atttagttac taacgattaa gcacatgaaa cgattactca ttgcacttat tcttacttgc
    18781 attccagcac attacgtatt cagctataca ctgactggat ggtttgaaga atcaaaaatt
    18841 tcagtttttg gaggagttgc cacgcatcat atagaaaaaa gaaatagcac tgaagatgta
    18901 aatctgatga actacctatc gtatagaaat gagttttatc taggcgttga tgcacatgtg
    18961 tacttacacg aacaatggca atttaacatc aaaacatacc tgaacagctc cttgcgaaca
    19021 gacgatgtgt ttgccaaact aatctatatg ccacatgaaa aaattggggt tactttgggc
    19081 tatgcatcta tccctgttca atatgggtta tatgagatac acgaacagcc acagtattat
    19141 actacaattc caaactcaga tgatgtatac gattttgcta aaaacgaatt gtttttagcg
    19201 ggtcttgcat ttccttttga aggaggtaaa ttctcaggaa ctatttactt acaaggagga
    19261 gttagttatc tgccaaacga tttttcgttc tcttggcaaa agaaatacga tagcaacgaa
    19321 cgtagattag tagaatatga tatccccgaa agttatgcat ttatcattca acccgaagta
    19381 gaaatacgtt gggaaatagc aaaaccaaca acagatacca gcataggtct tcaatttcaa
    19441 gggaaatgga attattcgaa acgaaatatc gattatacac gtaacatata cacttggact
    19501 acagacaaca agcaaacgga aataatttcg catccgaagc aatcaataga aggggtagat
    19561 tggaacgttg gcttgtttta tattttctaa aaaaccctat tttttgtagc tattgtacga
    19621 cactatttcg ttcaattttt tgcgtttttt gggtcgagtt tcttgagctg aataacccat
    19681 tacgattact agcaataccc ttttttcatc aggtatagaa agaatattct taatcttctt
    19741 ttcatcaaac cacccaatca tgcaggtacc caagccttcg tctgctgcag ctaaacaaat
    19801 atgggaagct actatcccaa tatcaatatg tggaaaatgt ttactttttg cccaaccgcc
    19861 aaaactagaa ctgatgtttg ctttttcttc tactactaca atctgaacag gggcttgttt
    19921 ggtaaaatga ttcattgcca agactttaga agccattgca tcagctattt gcaatcgctt
    19981 atctgtttca tttaccacca caaaatgcca aggttgtgca ttgcatgccg aaggagccaa
    20041 ctgagcagag gcgagtatcc gctgtaattt ttcatcttca ataccttttg gcaaaaaggc
    20101 tctatcgctt tgtcgggaag agacaagatt ataaaaggaa ttcataagca ttactattag
    20161 tattgtacaa aaatagaaat ttttctggta aaattcgttt aaaacttcca attcactaaa
    20221 atgcggatat ctgttttttg atttccataa atagtttctc cttcagagcc tatctcattt
    20281 ctgtcggcat accaagtatg tgcaaatttt gcataccaag taagttgagg gaaaggctta
    20341 tatacaacat ttacatataa ccgcattcct tgtccataat acatcggcac agaaaaagca
    20401 tataaaacat ctctttcgta tgcatacaaa cgattatcat agttaatggc atcaaacaaa
    20461 gcataacgag catttaacaa caaagggata ctagaaaatt cgtagccaac atcttgatat
    20521 aagagaaaac cataagtggg atgattgagt tgagaggcat actcattgga atgcatacct
    20581 gttttacaat gtaaatgttt aaaagggtta gtatcaattt gaaatcgcca ctcatttttt
    20641 gtatatggat ttaccattgg tgttgccatc tcttcgagta acacattccg ttgtttggat
    20701 gttgatttat attgacaaaa aaatgtggta tttcgagtcg gcgtatatgt tgcttgaata
    20761 aagccttgat aaccacgaga aggggtatta actaaatact ttaaccacgg aaaagaaaac
    20821 acatctagat aagaaaccag tttccattta cgaaatggca ataattctgt ccccataaaa
    20881 aagccttctt cgttatttgt actactactt tgtgaaaaac cattaccaaa tatactacta
    20941 tactcaggct ggtaatagcg atataaagct accaatccaa aacgagaaat aggctgaaaa
    21001 gaaagggtat gaacagtagc catagcacca tttttatcta tagccatttc gccagctaca
    21061 ttgaattgat gaaaacgagc ccgataatca atactagcag ttgaatagtg tttgccagca
    21121 aactcgtata tagcatatgg tttggatgag gggaaagacg gcttatcaag agaataagaa
    21181 aaaccagtaa tacctagttt taaaaacggg aagtggtatt gaatatttcc accaatcacc
    21241 atctgacggg ctacattctt tttcacaaaa tcattttccg tacgatgtat tccatctgtt
    21301 tttattgatg acaagcccaa tagattgttc aaatcatatt caagtgttcc atcaagagga
    21361 gtatttgaat aaaaggctgt tatttctaat tttttatgac caatagttgt tgctaatcca
    21421 ttgaaataca gagattcatc cagcgaggca ctcttactta ttccttcttt gtatatcgtg
    21481 ttagaaaata aagaagactt accaaatcgt tgacttgact gtaacactaa tccttgacca
    21541 aaattaagtt tataatgtcc tagtgcaata tgtttaaact tccagagaga actaataaac
    21601 aaatatggtg cataaaaatc aaaacccttg ttatagtgcc aatcgaaagg ctctcctgca
    21661 tctttttcca tacttactcc gatagataaa tactgtgacg aaaagcgata acgaaattgt
    21721 gtatataatg gattccccac atatcgttgt gcgtctgtgg caagatgcgt atagcctttt
    21781 ttcttttgta ttgtacagtc cgttcgcaac aatacttgat tttttccata tcgaaaagca
    21841 ttctgtaacg ttatgccctc tgattgtagt tcgctctttc ttacatatat aaaaggtaat
    21901 aaaaattgaa tcgtttcctc atcaaaacca tcaatcaatt gtaactcgta tacagtgtac
    21961 aaatcgccat aagaatagcg ataatacagt aaattttcaa tttgatatgg agagagaaaa
    22021 aataattgtt ctaattgttc tctagtgcat gtatttacat tcagtgggtt ttctgccaat
    22081 cgaagcaaat cctcgtatgt atcatctaaa acaacttcat catcactcat ttctaagttt
    22141 tctacgattc tatctattgt ttctaaataa gtattctgag ccatactaac gttggagggt
    22201 aaaaatccaa acccacaaag caagatgcac aaacattgac agtatttcat ggagtcctat
    22261 ttaccaaata taaatacaac acccgaactg accgacatgc ccaacactgg atgatattga
    22321 aatcctacat caaattgcaa aacatcaatg tttaatccta ccccaaaact tggttgtaga
    22381 tattgttgaa gagacattcc agtacgaaca atcacttctg gagcaattgt gtattcaata
    22441 ccagccgaca aggtagactc ttctctcaaa ggcttatgaa tttgggcaag taaaagcact
    22501 ttctgatcca tgtgataatc taaacctgca gtcaaatagg gctgtaggta ttcttcttga
    22561 taactggtct gcaagttagc aaaagtaaaa ttatatgcat gtatacctag gttaacagat
    22621 ggagtagctt ttatgattat ccctaattgg ggaacaaaag cactaaggta accctcttct
    22681 ggagatacat acacagaaac ataatcaact tgtgctccca acgaaaaatg agaagacaac
    22741 gccttagcaa cagacacacc agcaatcatc tcatgataaa gagagaaacc aaaaaaacga
    22801 tataagaacc caacattcat ccacggagta ggcatagaat agcgggcact aatcgaactt
    22861 aattctttaa caacaaatcg gttatgataa gataaagcaa gagttgagga agaaagaaat
    22921 gaagcagagg cctgattggc taaagcataa ttattatcca acagcatgac ataagactta
    22981 ccgagagcaa gacttcgtgg attctctatc tgttgcatca ctgacgcatg aatagggaat
    23041 acactgagca aaaaaaacac acagaataca tatcttttaa acaacacaag atctcctttt
    23101 ttttaatgaa agacaaaggt aatgttaagt attcttaatc tgcaatagag agtggtaatt
    23161 tttatcaaat aatactgtat ttttgcgtaa atatcgtgtc cttatattct cacgattatg
    23221 aaaaataaac tattcctctt gggcaacata atagtgttgt tgtctctttc tttaccaacc
    23281 ttagcaacat cgtacataca aactgctcaa ggttatattc ttcctgggat agtggttggt
    23341 aatgacacaa tccctatcgt agtactaaat gaacacatag tttatcctaa atatgtaaaa
    23401 aaacgcgatc aacaaaaata caataaatta gtacgagaca taaagaaggt atatcctttt
    23461 gccaaagttg tagggaaaga agtggaacgg gttaatcctt tattagaaaa acttccgcaa
    23521 aaagaacgaa aaaaatatat gcgagaatac gaaaaagccc tattcaaaca gtatgaaccc
    23581 gatttaaaaa aactaaccct aacacaaggc aaattattga ttaaattgat agatagagaa
    23641 tgcgaacaaa gttcttttga ccttattcgc gaatacagag gaggattgac cgcattcttt
    23701 tggcagggtt ttgctcgtat attaggagca aacctgaaag aagactttaa tgatgcaaaa
    23761 agtgaagacg caatgattaa tcggataatt atattgatag aaaacggaca attataacac
    23821 aaagaaatat gattcataaa aataaaatca ttatctttgc aacattagat gaacatacca
    23881 taccttaacc tatgctactc attatccttt ttatctgttt agggatagtt atctatctcc
    23941 tcaacaaata cacacgaaga gaaactccgg cgaaaatagc cgatacgcta acgccaataa
    24001 tagaagatac tgaagtatgt tgtggagcac atgaagtatg tgaaaaagac tccttattag
    24061 cttcgtcccc taatgctgtt tatttcgaag atgaagacct agatattttc cgatttcgcg
    24121 acgagaaatc atataatgca aaagaaattg cactatttga ggaagtattt tttacattgc
    24181 gtgaagatga aattgccgaa tggctaaaaa gcttgcaaat cagaaacatt aatctccctt
    24241 atgctatcaa agaagaagct ctccttattg tatctgaaag aagatacgca catactcact
    24301 aagaatatta tactagctca ccaaacatca tgaacaaaga atttccgtta attgcaaaaa
    24361 cattacaggg tctcgaagat attttagcaa aagagctagc acaaatagga gcaaacaata
    24421 tacaaaaaga aaaaagaggt gtctccttta caggaaataa cgagttgatg tacaaggcaa
    24481 atcttcactg tcgtacagca attcgtattc taaaacctat tttcacattt caagcaaaaa
    24541 atgcagatga aatatacgcc caaataaaaa aaatacattg ggaaaaatac ttttcagcga
    24601 aagaaagttt tgctatcagt gctgttgtat actcagaaac atttagccac tcaaaatttg
    24661 ttacgtataa agttaaagat gccattgtag attattttaa agaaaaattt aacgagcgtc
    24721 cttcaattaa gattacaaac ccagacgtat atttaaatat tcacattgca cacactactt
    24781 gcacaatatc actagatagt tcgggagaat cattacacaa acgaggctat cgaagtgctc
    24841 agacagaagc acctctcaac gaagtgttag cagcaggttt aattatgctc acaggatgga
    24901 aaggcgaatc ggattttctc gaccccatgt gtggatctgg aacactcctc attgaagctg
    24961 ctatgatagc acttaatatt ccgccgggag tctttagaaa agaatttgcg tttgaaaaat
    25021 ggaaagattt tgacagtgaa gtatttgata gagtgtataa tgacgatagc aacgaacgca
    25081 tttttaagca taaaatttac gggtcggaca tttcagctca agctctaaaa ataacagaag
    25141 aaaattgcaa gagtgcagga ttaagtaaat atattagctt gcaaaaagcc aattttatgg
    25201 aactccccac tccaacacaa aaaacactta ttgttaccaa tcctccttac ggagaacgca
    25261 ttgggggcaa tagcatcaat aagctatatg cagaaatagg cgaaacgcta aaacatcgtt
    25321 tccctggagc cgaagcctgg attattagct caaacatgga aggattttat aaaattggac
    25381 tccgcccatc acaaaaatat ctactcctaa atggttcgat cgaatgtgaa taccgcaaat
    25441 acgaactgtt tgatgggaaa atgaaagaca aaaaagcagc aataaatcag gaataatcaa
    25501 acgccactac gtacggtatc atttaacagg ctatcccaca tagtaaatgt taaatgatac
    25561 taaagaaagt ttgaattcaa atgttaaaat catatctttg ttttgttaaa aagaagatat
    25621 gaaatgctct aataaacata tttcaagcca gaggttggca ataccgcaag ttcccaatgc
    25681 ccttaacgta aaaacttaag aataagccta tgaataagcg aagtataatc cttttgtcag
    25741 tctttatggc aattaccttt tttgggttat tgtttttaca aataaagtac ctcaactctt
    25801 tgttagatat gcgtgtgcaa cattttaacg aagcagcaaa acgcagttta taccaactta
    25861 gcaagacact agaagaagaa gaaacactac actatttcaa tgaagccctc gctcaacgtc
    25921 cttctcaacg aagcaccagt tttatcgacc agacagtagg attgcaaagt gcaagttcta
    25981 ttactattca agaaaatcta caaggaagta ccactaccat tcagcaaaaa atatcaattt
    26041 caagctcgcc ctctacaaca cgaacagtac aacaaatatc agaaacactt caaaatcgat
    26101 taaaagaaaa ttttgctcaa aaaaagattt tacttgatga tattgctatg cgatggttga
    26161 aggaatctac tgacaaacca atcgaaaaac gtattgattt tacacatatg gaaaatcaat
    26221 taaaaaagga actagaaaac aatggaatca ctaccccatt caaagttatt ttgattgact
    26281 atacaggaga agaggtatac cgaacatcca attttaatgt gccaaatatg gaacattatt
    26341 tctcacaact cctatttcca aacgatttca atcctaaatt aaatattctg agggtatatt
    26401 ttccgtatgc aaaagaatat cttacacaac ccgtttcttt tatgatattt agttctgtac
    26461 tgtttacgat tattttattt ctagtatttt tagcaactat tatcttaata ttcagacaga
    26521 aacgtttggg agaaatgaaa acggacttca tgaataacat gacacatgag ttcaaaactc
    26581 ctatatcaag catttccttg gcggctcaaa tgctcaaaga tgaaggaatt acgaaaactc
    26641 ctgaggtatt aaaaagcatt tctaatataa tatacgaaga aacaaaacga ttaagttttc
    26701 aggtagaaaa ggttctagaa acatccatct tggaaaaaga gaaatctatc atgaaattta
    26761 aagaagccaa cgtccacgaa ataatcgaaa atgtttgcaa caatttttca atcaaaataa
    26821 gcagtaaagg aggcattatc aatacaaatt tacaagcatc agaatattgg gcaatggtgg
    26881 atgaaacgca ctttaccaac gtaatttata atctcctaga caatgcatta aaatatcgaa
    26941 atcttgacca aagtcctatc cttaaaataa aatcatggaa cgaaaaagat atcctttaca
    27001 ttagtgtaga agataatgga ataggaatca aaaaggaaca tttgaaaaaa atatttgaaa
    27061 aatttcatcg tgttcctacg ggaaatgtac acaacgtaaa agggtttggc ttgggtttgg
    27121 cttatgtaaa aaaaattatt gaaaaacaca aaggctctat tcgagcagaa agtgaactac
    27181 ataaaggaac aaaatttatc ataacaatac catcactaaa aaaaacacaa tatgaatgaa
    27241 caaaaagtaa agattcttct atgcgaagat gacgaaaatt taggtatgct tttacgcgaa
    27301 tacctacaag ccaaaggcta tgatacagac ctacttccag atggagatgc tggatacaaa
    27361 gcatttatga aaaacaccta cgacgtatgt gtattcgacg taatgatgcc caaaaaagat
    27421 ggatttacac tagcccaaga agtgcgagca gtaaataccg aagtgcctat tttgttttta
    27481 acagctaaaa ccctcaaaga ggacatcctc gaaggcttta aaattggagg agatgattac
    27541 attacaaaac cttttagcat ggaagaatta ctattcagaa tagaagcaat tctgcgtcgt
    27601 ataaaaggaa aaaaattaaa ggaaaaaatt gtatttaaaa tcggtaaatt tgtatttgat
    27661 tcgcaaaaac aaattctgtc aataggagat aaacatacaa aattaaccac aaaagaatca
    27721 gaactacttg ggttattatg tgccaatgct aatgacatat tagaaagaaa ttatacatta
    27781 aaaacaattt ggatagatga taactatttt aatgctcgaa gcatggacgt atacattaca
    27841 aaacttcgta aattattgaa agaagacgaa agtgtgcaaa taattaacat tcatggaaaa
    27901 gggtataaat taattgttcc tcaaggagtg gaataatctt taccttgaga tagaaaaaaa
    27961 acctcatcat tcaaaatatg atgaggtttt ttctatctat tttttaaaaa agaaattgta
    28021 tatttgtact ctcataagcc attcacaatt catatgcaat ttaagtttag tattttattt
    28081 cttcttatac ttgttacact cagttcgtgt agcatacagg ctcgaataaa caaagcagac
    28141 aaaaaatatg aattaggaga gtattacgta gcgggtaatt tatacaaaag aatttatcca
    28201 tctatacctt ctaaagaaaa agaattaaga gcaagtgtcg catttaaatc tgggaatgca
    28261 tttcgtcaaa ttaacgataa taaaagagca gaaacagcct atcgtaacgc catacgttac
    28321 aaatacaaag aacctttggt ctatttttat gcagcagaag ttgcccgcaa aaataacaaa
    28381 catacagatg ccatcaaatt ttacgaaacg tttttagaat cgaacccttc acatcgtgaa
    28441 gcattaaatg gcctttcttc gtccaaatta gtactatctg attggaaaaa acctactcga
    28501 tatgttgtta gcaaagaaga acttttcaat tctcgttttt ctgatttttc cccttcattt
    28561 gcaaacagca gaggagactt agtttttttt aattccacca gaaaattaaa taaaaaaaat
    28621 aaagcaagcc gcattacggg acaaaacaat aataatattt ttagttcccg taaaaatatt
    28681 caaggaaaat gggaagagcc actcctagta gaaggagata ttaattcaga atttgatgaa
    28741 ggcacctgtt cattttcatt tgatggtcaa gagctatttt ttacccgtag taaaattgaa
    28801 aaagggaaaa cactaggtac gggtattttc gtttctaaaa gaagtggagg ttcatggaca
    28861 tctccccaac aaatcatctt attacaagac tcgagtttga acattgctca tccagccctt
    28921 tctcccaatg gagactattt attctttgtt tctgatatgt caggcggata tggaggcaag
    28981 gatatttgga aagtggaaag aacaaaagat ggatggggaa ttccaaaaaa tcttggagaa
    29041 acaattaaca cagctggaga tgaaatgttt ccttcgttta aaaaaaatgg tacgttgtat
    29101 ttttcttccg atgggcatcc tggtttcggc ggattagata tttttaaagc cgaaccatcc
    29161 gaaaacaatg aatggatgat aagcaatatg atgagcccag tgaactcaga agccgatgat
    29221 tttggaatta cattcatgga aaaagaagaa aaagggttct tttcgagcaa ccgtgctgac
    29281 agacgtttct ttgacaatat atggagcttt gaactaccaa aacttgaata caaaatagaa
    29341 ggaaaagtaa cagataataa tggagatata cttggtgatg ctgtgataag actagtagga
    29401 tcgaatggaa ctattgagaa aatatctgtt cgtcgcaatg ggacattttc acaaataatt
    29461 gccccaaatg cgaactatat tttattggca tctgcacgag gatttttaaa tagctcgcaa
    29521 aaagtttcta ccgaaaattc gaataaaaac caaaccttta cactcaattt tcaattaaac
    29581 tcaatcagtc gacctgtgca aatgaataat attttcttta aatttggatc agcagaactc
    29641 acaccagaat ctgcagtagc tttaaacgaa ttggttaatc tactaaagga taatccaacg
    29701 gtaactattg aaattgcagc acatacggac atgattggta cagaaacagc aaacacactt
    29761 ttatccacac aacgagctca atcagtagta gattatgtaa aacaaaaagg aatagacact
    29821 gctcgtctta tcccaaaagg atacggaaaa aataaaccag ttgaagtaga taaaacactc
    29881 gccaaaaaat atactttttt atcagaagga gttatacttg atgaggaatt tattacatct
    29941 ttaccttctc agcaacaaga aattgctaat caaataaaca gacgtacaga gtttaaagta
    30001 ctaaaaacaa cctttcaggc ttattgaata gtgaaacaaa tggatatgaa caattagtaa
    30061 tatgaaacaa taccacgaat tacttcaacg agtactagat gaaggtacac aaaaagaaga
    30121 ccgaactgga acaggtacaa taagtgtatt tggacaccaa atgcgtttta aaatggaaga
    30181 cggatttccg tgtttaacca caaaaaaact gcatctaaaa tccatcatac acgaattact
    30241 gtggttttta aatggcgata caaatgtacg ttatttacaa gaaaatggcg ttcgtatatg
    30301 gaacgaatgg gctgacgaaa agggtgattt ggggcatatt tacggctacc aatggcgttc
    30361 atggcccacg tatgatggtg gttttatcga ccaaatatca gaagctgtac ataccattaa
    30421 aaacagccct gattcacgcc gaataattgt aagtgcctgg aatgtggccg atttgcccaa
    30481 tatgaactta ccaccatgcc acgctttttt tcagttttat gtagcgaatg gcaaacttag
    30541 tttgcaattg taccaacgaa gtgccgatat ttttttaggt gttccgttta atatagcatc
    30601 atacgcccta ttacttcaaa tgatggcaca agtaactggg ctacaagcag gtgattttat
    30661 tcacacattg ggagatgcac acatttacac caaccatctg gaacaggttc aactgcaact
    30721 tacacgtgaa cctcgccaat taccaaccat gaaaatcaac cctaccataa catctatctt
    30781 tgattttcaa tttagtgatt ttgaattagt aaattataat ccgtatcctc atatacctgg
    30841 agctgtggcg gtataatatc gattttatat gacaaatatt gctattatag tagctatcga
    30901 caagcaaaat ggaattggca aaaacaacca attactatgc catttgccaa acgatttaaa
    30961 gcattttaag caactaacct cagggcacac aatcatcatg ggtagaaaaa cttttgaatc
    31021 attgccaaat ggagcgttac ccaatagaaa gaacattgtt atcacacgaa atcaacatgt
    31081 tacttacaaa gactgtgaaa tagcctattc attagatgat gccattagta aaaatgctga
    31141 aaaaaatatc gtttttatta tcggaggggc atcaatctac gaacaagcca tatcagtggc
    31201 tacaatgttg tatatcacaa aaattgatgc tacattcgat gctgatacat tcttccctca
    31261 catcaacaaa aatgattgga ctcttgtaaa aaaagaatca catcaaaaag acgataaaaa
    31321 tccctttgat tatacctttg aaacatgggt acgaaaaacg agtagttcac aagacatatt
    31381 aggcatctaa attgttcatt gtcttgcaat taatacaaaa tagagcagtt aacaaaaaaa
    31441 tacaaaaaaa gttgatatat ctataaaaaa atacacttaa tttgttacct ttgcgaaaca
    31501 ttaaaaaatc tctaataaga tggaaaaaag aaaaataaaa cgtgccttag tctctgtatt
    31561 tcacaaagag aatttggata aactaatttt aaaactacat gcagagggcg tagaatttat
    31621 ttcaacagga ggaacaaaat cttttataga atcattagaa gttccatgca cagcagtaga
    31681 agacttaaca ggctatccat caatcttagg tggtcgtgtt aaaacattgc atcctaaagt
    31741 atttggagga atattaagtc gcagaaaatt agaaggcgat atgaaccaat tagctgaata
    31801 cgacattcca gaaattgatt tagtaatcgt tgacttatac ccattcgaag ctactgtagc
    31861 atctggagct agcgaacaag aaattattga aaaaattgat attggtggca tatcccttat
    31921 ccgtgcagcc gcaaaaaact ttgccgatgt aattattgtt gcatcgcaag ctcaatacgc
    31981 tccactatac gatttgctag aagaaaatgg agctgaaagt tctattgaac aacgcaaata
    32041 ctttgccaaa gaagcctttg ctgtatcttc atactacgat actgctatct tcaactattt
    32101 tgatggatac gaaaatagtg ctttccgtca tgcagaaaat accatgaaag ctttacgata
    32161 cggcgaaaat cctcatcaac gtggattttt ctacggaaaa ttcaacgata tgtttgagca
    32221 attgcaaggg aaagaaatat cgtacaacaa tttattagat attgatgctg ccgttagttt
    32281 aattaatgat tttgacgaaa ttacatttgc catcttaaaa cacaataatg cctgtggtat
    32341 tgcatctcgt agcaatttac tcgacgcatg gaaagatgcc ttagccggtg accctgtgtc
    32401 agcatttggg ggtgtgttaa ttaccaatgc aatcattgac caaactacgg ctgaagaaat
    32461 caataaactt ttcttcgaag taattattgc tcccgactat gacatggaag cattagaaat
    32521 attatcacaa aagaaaaatc gtatcatttt aattcaaaaa gaaactaaaa gcaaaactac
    32581 tcaatttcgc tcattactca atggcgtctt agtacaagat aaagatttga gcattgaaac
    32641 cgaaaaagat tttaaagttg ttacacaaat tgaaccgtca tcatcagaaa ctgaagacct
    32701 tatttttgca aataaaattg taaaacatac gaaatcaaat gccattgtat tagctaaaaa
    32761 caaacaatta tgtgctagtg gcgtaggaca aacatcacgt gtagatgctc tgcgtcaagc
    32821 catagaaaaa gcaaattcat ttgaaatgag tttgaaaggt gcagtaatgg cttcagatgc
    32881 atttttccca ttccccgact gtgtagaaat agcatcacaa gcaggaatta catctgttat
    32941 tcaaccagga ggttcaatca aagacgattt atctattgag ttttgcgaca acaataatat
    33001 gtcgatggta accacaggaa tcagacattt taaacattaa aaatttaaca aacatagaca
    33061 catgggatta ttttcattca ctcaagaaat agcctttgac cttggtacag caaataccat
    33121 catcattcaa aatgggaaga ttgtattaga cgaaccatct attgttgccc ttgataaacg
    33181 taccgacaaa ttgattgcca ttggagaaaa ggcacgtcaa atgcaaggta aagagcacga
    33241 aagcatacgt actgttcgtc cattacgcga tggtgttatt gccgattttt atgcagccga
    33301 aattatgatt agaggcatga taaaaatgat aagtaacaaa aattcatttt ttgctccttc
    33361 tcttaaaatg gttgtttgta ttccatcggg aagcaccgaa gttgaaatac gtgccgtacg
    33421 tgactcttct gaacatgctg ggggtagaga agtatatatg atatacgaac ctatggcagc
    33481 agcaatgggt ataggtcttg atgtagaagc accggaaggc aatatgattg tagatatagg
    33541 tggcggtagc accgaaattg ccgtaatatc tttaggagga ttagtatgca accgttctat
    33601 tcgtattgct ggagacgatc ttacctcgga tattgtagat tatatgggaa gacaacacaa
    33661 tataagaatt ggagaaagga cagctgaact aattaaaata aacgtcggat cggcactaac
    33721 agatcttgaa gatgctccag aagattacat tgtacgcgga ccaaataggc tatcagctct
    33781 accaatggaa gttcctgtat cataccaaga aatagctcat tgtttagaca aatctatatc
    33841 aaaaattgaa attgcagtgt taggggcatt agaacaaact ccgcccgaat tgtatgctga
    33901 tatcgttcgc aatggtatct acttagcagg aggtggagct ttactaaaag gacttgataa
    33961 acgtttgcaa gataaaatca atattccatt tcatattgca gaagatccct tacgtgcagt
    34021 agcaagagga acaggaattg ctcttaaaaa tgttgataat tattcattct tattaaggta
    34081 atacgtcact atacatgaga gaatgtacta ttgtcggcag aaatatttct tttttgctga
    34141 caatttgttt tttatacaga tagataggta tttactcaag gttaatcagc atcatgtaat
    34201 tatttatgga aaatttactc cgttttttag ctaaagcaag ttctttttta ttgtttgttt
    34261 ttttacaaac agtagcctta ttgttgcttg tttctcacaa caactaccag caatctattg
    34321 cacttagttc ggctaatact atcacgggaa atatgtatgc cactattcaa tcttttacag
    34381 aatactttca tcttcagact acaaatatgt tgcttgccga agaaaataca gccctacaaa
    34441 atgaaaacaa tatactgcat caacaactaa tgaaacaaaa tctgtcttcc attaatcaag
    34501 atacagtcgc attatttacc tatatttcgg ctaaagtaat aaatagctct gttaacaaac
    34561 aacaaaatta cattacacta aataaaggtt ctaaagatgg tataaaaccc gatatgggtg
    34621 taataaatag ccaaggagta gtgggtatcg taagtgctgt ttctaaaaat tttgctgtgg
    34681 taatgcctat tattaatcct cgtatcaaaa taagttgccg tattgccaaa aacgattatt
    34741 atggttctct tttatgggaa ggaagaagtc cattttttgc caatttaaag gaaataccag
    34801 agcatgtaga attagaaaca ggagactcta ttattacaag tggtttttct tctatctttc
    34861 cagaaggcat accagtaggt acggttgaag aagtaaatac atcaaaatat gataattttc
    34921 atgatataaa gatacgatta aacgttaatt ttcaaacgat tacgtacgtt aatgttatta
    34981 attttaatca aaaaaccgaa ttaaacaatc tcgaaacaca acaactacaa tgatactgac
    35041 aattgccaga aatacacttt tttttgtagt actcgtttta gcacaagtat tattgttcaa
    35101 taatattaac tttttgggat ttataaatcc ctatgtgtat gtgctattta ttttaggatt
    35161 acctgttgct ttaaacaaaa aaagcataat ggttgtggcg ttctgtttag gattactcat
    35221 tgatattttt acaaacacct taggaataca catttttgca tctgttttaa ttgcatatct
    35281 acgtaacttt tggattgatg ctctattttt tcgaaatgat ttcgattttg tatctccttc
    35341 tatcaaaaat tttggttttt tcaattttgt caaatacgcc tttgtcctaa tttttgtaca
    35401 tcatttggct ttgtttatat tagaagtact tacttttaat cattttttac taatgctatt
    35461 acgtgtattg tacaattcta ttgttagttt attactcatc attagttttg agcttatcaa
    35521 gtcgaaatga aagattcaat aaaaaatcaa gaacttagaa atagacaata tataattata
    35581 ctcattgtat tgtctgttgc ttttatatac attatccaat tgttttatat tcaaatcgca
    35641 gatagttcgt acaaagaaaa tgcggatagt aatgcttttt taaaaaaggt tatctatcct
    35701 tctcggggtt tattgtacga tagaaatgaa caattatatg tatctaatca gccagtatat
    35761 gatttgatga ttattatgcg tgaaatacag ggatttgaca cactctcatt ttgtaaaatg
    35821 ctcaatatca atcaagaaga atttaacaat agaatagaag aaataaaaga caagcgtaaa
    35881 aacagaggat actctcgttt tactcctcaa atatttatgt ctcagttaag tgttgcagaa
    35941 tatggcaaaa ttcaagaaca gttgtataaa tttagaggag tatatgcaca acaacgtgta
    36001 ctacgcaaat acaccacccc tcatggagct catgcaattg gctctgtagg agaagtaaat
    36061 cagcaacaaa tagatgctga ttcatattat tctcgaggtg attataaggg tcaatctgga
    36121 gtagaaaaag aatatgaaac gtatttaaga ggagaaaaag gagtagaaat attattaaga
    36181 gatgctcatg gtcgtattca aggggtatat aaaaatggag aaagaaacaa ggctccaatt
    36241 gcaggcaaaa atctgaccat ggctattgat atggggttac aagagtatgg cgaacaacta
    36301 atgattaaga aattaggcag tattgtagcc attgaaccct ctacaggaga aatattagcc
    36361 cttatttcaa gcccatcata tgaccctcat ttgttggtag gaagagaacg ttctaaaaat
    36421 tataatgcat tgctgaaaga ccctttcaaa cccttatttg acagacccct aatggcaatg
    36481 tatcctccag gctcaacatt taagttagta aatggattaa tctaccagca agaaggcgtt
    36541 ataaccgaag acacacgttt ttcttgtagc atgggttact actatagtcg gacacgtaaa
    36601 ttaggctgtc atccacatcc taacaattta gatttacgcg aatctgttca acattcgtgc
    36661 aatgcgtatt attgtcatgg attgaaaggt ttattagatt ctccaccaag tggtatttct
    36721 actaccgaat cctataatca ttggagaaat catgtgttat ctatgggttt tggaaaaaaa
    36781 ttaggagtcg attttcctaa tgaaaaggga ggtttgattt tagaaagtaa tcgctatgac
    36841 cgaatatacg gaaaaaacaa atggaaatca tccactatta tctctatcgc aattgggcaa
    36901 ggagaagtgt tagcaactcc cattcaaaca gcaaatttgg cagcactaat agcaaacaga
    36961 ggatattttt acactcctca cattatcaaa aaaattgaag gagatagtat tgataaacgt
    37021 tttagagaaa aacatcaaac gacaatagaa caaaaatatt tcgatcctat tattgatgga
    37081 atgttaatgg ctgtggaaca aggaacaggt agggttggca gaatagaagg tattgaaatt
    37141 tgtggtaaaa caggaacagc acaaaatcca cacggaaaag accactcaat ttttatagca
    37201 tttgccccca aagatgatcc gaaaattgca gtcgccgtat atgtcgaaaa cgcaggattt
    37261 ggagcaacat ggtcggcacc cattgcgagt tttgttatag agaagtattt aaatgattct
    37321 ataaacaagt cgagaatatg gttagaaaac cgaattttag aaggagattt gataaatgtt
    37381 cagaagacaa ataactaata tttggaactc tttagactgg ttcacagtcg ccttatatgc
    37441 catactagta attatgggat ggttcaacat ttatgcagct gtttataatg aggagtttgg
    37501 cagtattttc gacatatcac aaaagtatgg gcgacaatta ctgtggattt tggcagccat
    37561 tgtactgatt gttgttattt ttttgataga caataaaata tacgtatatt tttcatatcc
    37621 tatttacata gctgtgatgt tgttactaat atcagttctt ctgtttggag ttgagataag
    37681 tggctcacgt tcatggtttc agataggaga atttaagata cagccagccg aattttcaaa
    37741 gtttgctaca attttggcta tcgcaaattt tttgagcgaa cccggaagaa ggctaatgaa
    37801 attgccaaat tttttagttg ttttgacgtt aatagctata cctacaattt taatactact
    37861 tcaaaatgac acaggttcgg cattagtttt cacttcgctt gtgtttatgc tttatcgaga
    37921 gggtcttccc ggatggattt tattgattgc cttttttgct atcgtcttgt ttattatagc
    37981 actcacaaag ccattttatt gggttgtagg tttagttata attgcatgtt ggatttatgc
    38041 atatttaaaa tacaacgcaa aacgagattt tatctacttt gggattttta tgctaatgat
    38101 aatcggtgca
//
LOCUS       DAOSRP010000003        30959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1621_length_30959_cov_5.412309, whole genome shotgun sequence.
ACCESSION   DAOSRP010000003 DAOSRP010000000
VERSION     DAOSRP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 30959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30959
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1621_length_30959_cov_5.412309"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLJ82_00380"
     CDS             complement(<1..673)
                     /locus_tag="PLJ82_00380"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOR40000.1"
                     /translation="MKVAIVGTSGAVGQEFLRVLAERNFPMDELVLFGSSRSAGSEYE
                     FKGKKLVVKELKHGDDFKGVDIAFTSAGAGISNEFAADITKFGAVMIDNSSAFRMDTD
                     VPLVVPEVNAADAKNRPRNIIANPNCTTIQMVVALQAIENLSHVKRVHVSTYQAASGA
                     GAAAMDELELQYKQVLHGETPTVSKFAYQLAYNLIPQIDVFTDNGYTKEEMKMYHETR
                     KIMHSD"
     gene            924..3236
                     /locus_tag="PLJ82_00385"
     CDS             924..3236
                     /locus_tag="PLJ82_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOR40001.1"
                     /translation="MKNILIIFLCFLSASTLAQVNETFSDGNFSHAPIWEGNASNFVI
                     NSNAQLQLNAPAEANKSYLVTNSNVTTNAEWKMYGKMMFTPSSANYARWYLIADKQDI
                     TGSLNGYYLLIGNTSKNICLYKQTGTTSTKILDTPINRLNGTTNEINIRITRSSEGLW
                     QLFTQLPTENSPILEGTTTDNTHKAALYTGIVCYYTATRNTGFIFDDIFISGETYEPP
                     ILPSYKASFGDIVFNEIMANPNPPFGLPNEEYIELYNNTSEAINLENYVLKGFSKTCV
                     FPTYVLAPHSYVVVCSTKAFPALEMYGNCLALDKFPTLTNAGGYLELFNAENTILSWV
                     DYSENWHTDSFKKNGGWSLECLDAHNLIGNNTNWNSSINYQGGTPNGANSISGINIDT
                     SELSVINTSIESNSSFVLYFNKPMSVNLLTQTDIITIYPMREISSIEFLSIKQDAIRI
                     TLTNPLLLHEQYTLNITDVEDINKNKHSIVSRLAIPELPSNQDVVINEVLFNPKSEGT
                     DYVEIVNRSQKVIDLRDIMLTNRKQDGQLAVFPVLSEKGYLLFPGDYCLLSTSKEGVC
                     NYYECPDDIHFVTVPSFPSMPDDKGTIVLTTLSNILIDEFSYSEKMHHTLLSNREGIA
                     LERLHYDITTQDASNWHSASFTCGYGTPGYQNSQFTALIQPTASFQITPKSFSPNNDG
                     NDDTATIYYSFDEAGYFVTIRIYNSNGNCVRNILQNNLSSTEEKTSWDGTDDNGKLLP
                     IGIYIITIEAFTPTGKRFKSKEVIVLAAKI"
     gene            3307..4821
                     /gene="gpmI"
                     /locus_tag="PLJ82_00390"
     CDS             3307..4821
                     /gene="gpmI"
                     /locus_tag="PLJ82_00390"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOR40002.1"
                     /translation="MGKKALLVILDGWGIGDKSKSDIIYNTPTPYWDKLVATYPHSQL
                     QASGESVGLPDGQMGNSEVGHLNIGAGRVVYQDLVKINLACRNNTILDNPAVVNAFSY
                     AKQHNKSVHFLGLVSDGGVHSSLEHLFKLCDIAKTYEIDNTFIHCFMDGRDTDPRSGK
                     GFISELEAHTKKSAGKIASIIGRYYAMDRDKRWERVKESYDLLVNGIGEPQTDMIAAI
                     QKSYDNGVTDEFIKPIVNKAVDGKIKEGDVVIFFNYRNDRAKELTIVLTQKDMPEFGM
                     QTIPNLHYCTMTPYDASFKGMNILFDKDNVNNTLGEYLSGLGKSQLHMAETEKYAHVT
                     FFFNGGREEPFAKEERILINSPKVATYDLQPEMSAYEVKDALVAEIKKQKHDFIVVNY
                     ANGDMVGHTGVYKAIETAVKAVDKCLEETIETAKANGYEAIIIADHGNADNALNADGS
                     ENTAHSLNPVPFVYITEKKNTSVKNGILADVAPSILTIMGIPQPKEMTGKTLID"
     gene            4930..5088
                     /gene="rpmH"
                     /locus_tag="PLJ82_00395"
     CDS             4930..5088
                     /gene="rpmH"
                     /locus_tag="PLJ82_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR40003.1"
                     /translation="MKRTFQPSNKKRKNKHGFRERMASANGRRVLASRRAKGRKKLTV
                     SDELSLRK"
     gene            5238..6047
                     /locus_tag="PLJ82_00400"
     CDS             5238..6047
                     /locus_tag="PLJ82_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOR40004.1"
                     /translation="MKRKPLNIFVKHLLIILGTFIAIGIVAFISLSIYTQHGKQETVP
                     NVKNMSITDAEKILNSNNLSYEIVDSIFNRALTPGFVAEQDPIPSSIVKKNRKIYLTI
                     NAKGVQLLPLPNGINMSLRQAKSLFEAADFVIKKIEYKNSEYKDLVLDVKLNNSSIPI
                     GKTIPAGSALTLVVGNGFNGELAIVPDVRNLSYEKATQLANESGFNLDEAIYDEVPLN
                     TADEKKYSIYRQEPRAKASLATGEFIKVWLSKTPQEYNTEEEISLEEDILF"
     gene            6054..7109
                     /locus_tag="PLJ82_00405"
     CDS             6054..7109
                     /locus_tag="PLJ82_00405"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR40005.1"
                     /translation="MIEELEEDLLELTDPSESPLFEHHRFVSDNGQGLVRIDKFLHDR
                     IVNSSRTKIQEAADAGNILVNGTAVKANYRVKPKDVITIVMAYPPREVEIIPQNIPLT
                     IVYEDDDLMVINKPAGMVVHPGHGNYDGTLVNALAYYFRNNANYNATDPRLGLVHRID
                     KNTSGLLLIAKNEYAKINLAKQFFDKTTERVYKALVWGNFQENEGTIKGNIGRSLKDR
                     MQMDVFPNGEQGKHAVTHYKVIENFNYVSFVECRLETGRTHQIRAHMKYIGHPLFNDE
                     RYGGNSILKGTTFNKYKQFVQNCFDTCPRQALHAQTLGFIHPISKEKLFFTSAIPEDM
                     KDLLEKWRNYTTSYVIN"
     gene            7123..7791
                     /locus_tag="PLJ82_00410"
     CDS             7123..7791
                     /locus_tag="PLJ82_00410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOR40006.1"
                     /translation="MKTRQLILLALSLFLSQLSFASTFKVNGTILDSKKRGISNMVVS
                     TTQTTSGAITNNKGYFSIHAANPHDTLIINVLQDKILIPINEIKNIKITIADTITAEE
                     IIDETVNIGYQRIKKEDVTINPNTISGERLLATGETNLNEALLKVMPSIRQDRNGMLI
                     IRATQSLTQQGPPLYVVNGTPTNTVSNIPLSEIKDVQVLKDGSACAIYGVQGNNGVIL
                     INLK"
     gene            7862..8494
                     /gene="pyrE"
                     /locus_tag="PLJ82_00415"
     CDS             7862..8494
                     /gene="pyrE"
                     /locus_tag="PLJ82_00415"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOR40007.1"
                     /translation="MENLSTLTASKLLKINALKLQPTNPFTWASGWKSPIYCDNRKTL
                     SYPDVRNFIKLQMTRIILEKYPDVQAIAGVATGAIAQGALVAAELNLPFVYIRSSKKD
                     HGLENLVEGDLQTGQKVVVIEDLISTGGSSLKAVDAIRNNGCEVLGMVAIFTYGFPVA
                     QQQFSEAKVELTTLSNYEAVLALALETNYIKESDITTLQEWSKSPSTWNA"
     gene            8505..8912
                     /locus_tag="PLJ82_00420"
     CDS             8505..8912
                     /locus_tag="PLJ82_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOR40008.1"
                     /translation="MTQFDSEIKKINATSEIVFNKLTNLENLSQLEGSMPHEKVKILK
                     ITENTCLFEVEMIGEIGIKIANKEPHHLILFESQQSPLAFNLSIYLTEQNEHTELQIQ
                     LKADLPMMVKMMVSEPINKFVNILADTLTRLSY"
     gene            8917..10254
                     /gene="argH"
                     /locus_tag="PLJ82_00425"
     CDS             8917..10254
                     /gene="argH"
                     /locus_tag="PLJ82_00425"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOR40009.1"
                     /translation="MAQKLWEKDTKVNSEIERFTIGKDREMDMYLAKYDVIGSMAHIT
                     MLESIGLLTPKELTTLLLGLKEIYKEIESGHFIIDENVEDIHSQVELVLTDKLGDMGK
                     KIHSGRSRNDQVLLDLKLFSRAKIQEIATETHLLFNTLLTQSEQYKSVLMPGYTHLQI
                     AMPSSFGLWFGAYAESLVDDMQLLLAAFKVCNRNPLGSAAGYGSSFPLNRQKTTDLLG
                     FDSMNYNVVYAQMGRGKMERTVSTALASIANTIAKLAFDSCLFTSQNFGFIKLADEFT
                     TGSSIMPHKKNPDVFELTRAKCNKIQGIPQQINLITNNLPSGYFRDLQIIKELFIPMF
                     DELIECIRMTRYIIEKVEINTHILDDKKYQAIFSVEEVNKQVLSGIPFRDAYKNIGLA
                     IEKGDFHPDTSISHTHEGSIGNLCNEEINALMNEIISAFNFKSIQEAEAKLLL"
     gene            10266..11033
                     /locus_tag="PLJ82_00430"
     CDS             10266..11033
                     /locus_tag="PLJ82_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR40010.1"
                     /translation="MLFSSIIFGPIKSRRLGNSLGINLLPTDAKICSFDCIYCECGLN
                     DSTKKGNIPTREAVKNALSLELEKLKKQDITIDTITFAGNGEPTMHPDFKEIIDDTLI
                     IRNQLAPDAVISVLSNAWHITNPKVYGALLKVDNNILKLDSAIKETILQINHPNNTSF
                     EVSELIANLASFQGQCIIQTLFVKGNHKGNLIDNTTEEEINAWLNALKTIQPKQIMIY
                     SIDRETPIKSLEKIPSSTLKAIASQAKKLGFNISISV"
     gene            11030..12091
                     /locus_tag="PLJ82_00435"
     CDS             11030..12091
                     /locus_tag="PLJ82_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40011.1"
                     /translation="MKTAKRILICPLNWGLGHATRCIPIIHKLQQEGNILLIASSGCA
                     GELLQQEFPTLEYIHFPGFSITYSKHHSQTLRIVLSFPKIIYYSFKEHFVLKKLIKIH
                     DINCVISDNRFGLWNKDITTAYITHQLNIKLPKHVKIFEKLTWYIHRYFIEKYNYCII
                     PDNQQSTLSLAGDLVHQRKLPKNAFFCSPLSRFSKSIQNTSTKFQSNILIILSGIEPQ
                     RSILEKLLLQQLTKINQKAILIQGLPNETKYKKNIGNITIISYVLHQELQELINNTDL
                     VICRSGYSSIMDLVAINKQAIIIPTPGQPEQEYLAKYLHEKKFFIAQQQDNIDILKGM
                     EESKSTKIPDMKFYLQLPF"
     gene            complement(12092..12748)
                     /gene="upp"
                     /locus_tag="PLJ82_00440"
     CDS             complement(12092..12748)
                     /gene="upp"
                     /locus_tag="PLJ82_00440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOR40012.1"
                     /translation="MRTHNLALQDSLLNTFLEQIRSKEVQKDRMRFRRNMERIGEVMA
                     YEISKSLAYSTKNIATPLGIASVNTVDNDIVLGTIFRAGLPFHQGFLSYFDNADNAFI
                     SAYRKYKDALKFEVFIEYIASPDLTGKTLILVDPMLASGSSMELAYNALLTRGKPKEV
                     HFATIIASQYAVDYLSNHFAEESNISLWSAAIDPELNAHSYIIPGLGDAGDLAFGEKL
                     "
     gene            complement(12745..14034)
                     /locus_tag="PLJ82_00445"
     CDS             complement(12745..14034)
                     /locus_tag="PLJ82_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR40013.1"
                     /translation="MDISFTTKEQVAIDSTIPVICYSREHRIPKSFVIEPEGLLFKRG
                     IKDIQPEVTEWNGTKIFFQSQSADVSIPFDIFAASFYLLTRYEEYGSLDLDEHGRYKA
                     ENSLAYKHQFLDYPLVDHWAYLLDQMLADYFPMYTIKSKSRFRFCPSIDIDHVYKYKS
                     KFLSINIIRLFQKLITGKYEDFRYHLNVILNRAPDPYFNVEYIVDMHNDYNLRPIFFL
                     HVGPMGKYDGYSLYNPFNIIKELFYRTLIVGLHPSYFASTNLHQMKKEKKRLESMTRV
                     RVFLNRFHFLKFTFPISFEYLESLSIARDYSLGYAKYTGFRASTCTPFLFYNLTKDYI
                     SGMRLHPIVLMDVTLQKYLHKEATDLFGIMSYYAEVIKKYNGEFVTLFHNESLSDEDQ
                     WYGWREEYKKSVQYVALLELHSIEVAKEKLANQHMGV"
     gene            complement(14123..14818)
                     /gene="radC"
                     /locus_tag="PLJ82_00450"
     CDS             complement(14123..14818)
                     /gene="radC"
                     /locus_tag="PLJ82_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOR40014.1"
                     /translation="METKLLSIKEWSQEDRPREKLLEKGIAALSDAELIAILIGSGTR
                     NETAVELAKKILLLANNDLNQLSRLSVKELTKSTKGVGNAKAISIVAALELGRRRKRI
                     DANQLVTITSSEHVASIFAPLLADLIQEEFWVLFLSRANKVVAKMKISIGGIHQAVVD
                     TRIIFKYALQHAASGIVLCHNHPSGALLPSQEDKNITEKIKQGAKLFDMNVLDHIIIA
                     GDSYYSFADHNIL"
     gene            complement(14954..15205)
                     /gene="rpsT"
                     /locus_tag="PLJ82_00455"
     CDS             complement(14954..15205)
                     /gene="rpsT"
                     /locus_tag="PLJ82_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOR40015.1"
                     /translation="MANHKSSVKRIRQTEKRKLHNRYYAKTARNAVRKLRALREKEEA
                     VALFPKVVAMLDKLAKKNLIHKNKASNLKSKLAKKVNSL"
     gene            complement(15230..15301)
                     /locus_tag="PLJ82_00460"
     tRNA            complement(15230..15301)
                     /locus_tag="PLJ82_00460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15266..15268),aa:Glu,seq:ttc)
     gene            complement(15410..16036)
                     /locus_tag="PLJ82_00465"
     CDS             complement(15410..16036)
                     /locus_tag="PLJ82_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40016.1"
                     /translation="MNNMDVSKRIAQFFTIFFQPMLIPIYGVLLLIDSGYLYLYTAIG
                     QWLVLGIAIFFSVFVPAVSAVILYKMGIVTSPQIAKREERFYPYLISVISLGMLAYVY
                     TILQVPQFLLYLVYGLLVSVVSVFFVNMWWKISAHMGGIGGLLGGVLTTTYYFKENAM
                     WLYIALFIISGMVAFSRLKLKAHSLGQLFAGFLVGLVCVGGVPLFLFL"
     gene            complement(16040..17929)
                     /locus_tag="PLJ82_00470"
     CDS             complement(16040..17929)
                     /locus_tag="PLJ82_00470"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /protein_id="HOR40017.1"
                     /translation="MKIHMGDYCLDIYNYHRRNTDQVLIGTVWVGSKYPIRVQSMTNT
                     STMDTDASTAQCEELIDAGAELVRLTTQGVREAQNLYFIRKNLLAKGYVVPLVADVHF
                     NPKVAEEAARIVEKVRINPGNFVDSIKTFQQYEYTDEEYAKEIAKIEEKLIPLLEICK
                     EHSTAIRIGVNHGSLSDRIMSRFGDTPQGMVESCMEFLRICRKHNFLRIVISVKASNT
                     MVMVHTVRLLVATMQQEGMRFPLHLGVTEAGDAEDGRIKSAVGIGALLIDGIGDTIRV
                     SLSEHPKNELPVANKMVDYINQVAKAPTIEPFIYHNNFFKPFAYNRRKSIPVGPIGNT
                     HVPIVIHSLSDTENGIDVTATKPDFIYVGSSIVNTYPTPKIIDAAVWRKEENTYPMFS
                     IETYTNIQMCDADIVFLSMKSTEITKEICEFISATTRVILIVQLLSENPVGEGRKVFA
                     TLHYYQVENPVVLQRAYNEEDIEMLQIKSSVDIGSFFLDGFGDGIMLENKQKIQDTVS
                     LSFSILQATRARMSKTEFISCPGCGRTLFDLQQTIAHIKRATSHLNHLKIGIMGCIVN
                     GPGEMADADYGYVGAGKGKISLYKKQECVQRNIPEKEAVQRLIQLIKDNGDWYDNGAE
                     NELMQ"
     gene            complement(17926..19215)
                     /gene="murF"
                     /locus_tag="PLJ82_00475"
     CDS             complement(17926..19215)
                     /gene="murF"
                     /locus_tag="PLJ82_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOR40018.1"
                     /translation="MNISDLYELYLAHPHITTDSRNCPSNSIFFALKGDNFNGNQYAM
                     QALEKGAAYAIIDEPLQIHDARCIVVDNVLLTLQDLARFHRKKLTIPVLGITGSNGKT
                     TTKELCAAVLRQKYKVHYTKGNLNNHIGVPLTILGITPDVEFAIIEMGANHPGDIQEL
                     VEIALPNYGIITNVGRAHLEGFGSFEGVIKTKKELYDYILKANGKIFIDSDNSYLQEM
                     ALGTNQISYGTNYENYVSGRIKQVSPFVSFEWTKNDDSTYFIQTALIGEYNLSNLLAA
                     VAVGVYFGVSALQCKDGIEAYVPENNRSQYMKTSKNELILDAYNANPTSMEAAIRNFK
                     NITTSLPKMLILGDMFELGKEAQNEHQKIVDLLDLLQFDKVCLVGSLFSQTKSNFTTL
                     TNGDELVAYLQEKDFQGLSILIKGSRSMKMERVVEFL"
     gene            19414..20721
                     /locus_tag="PLJ82_00480"
     CDS             19414..20721
                     /locus_tag="PLJ82_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOR40019.1"
                     /translation="MTKHLISFFFAILLIGLASCLNEKEDLSNNPHPSAVSIQTNTLA
                     PDIESTVFTIDETNLAISNSDSIAYNSRIDKLVPVFSFAEGVEQILINGTIWNTTDSI
                     DFSSPVIIQFKAKDKLTTAEYTFKVNKHTVDPEAITWTKRADNFTNLTNIEEMAGLWF
                     QSQAILFVQKGNETFVLKSNNGQTWTEAISSNIPLEVSTILVHKNAILAISKNHHLIT
                     SVDGINWSIISSSDEISFINILCSLQNKAWIVGDKNNITYIYSYNSESETITEVTTVS
                     DKLPYIEVSKAMMKTKNGIEKSVLISGKTNNDTPVYSVFSSMTGDYWVNVITETASLT
                     FGERIHATAIWLDSKLLLFGGSTANNNNLLDVYISSDEGYTWEIASSNKQLPVEIGAR
                     KKMIGFVDSNNTLWLIGGIDALGNIQSDVWQGRLNKFDFIKQD"
     gene            20723..21169
                     /gene="coaD"
                     /locus_tag="PLJ82_00485"
     CDS             20723..21169
                     /gene="coaD"
                     /locus_tag="PLJ82_00485"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR40020.1"
                     /translation="MSKTALFPGTFNPFTIGHYSLVKRALTIFDKVIIAIGINSTKEN
                     NLDEIEENKKQIADLFKNENVKVITYTNLTADIVKAEKASCIVRGIRGISDYEYEKNM
                     ADINKELFEIETVFLFSDLKYAHISSSLIRELKRFGKEYKHLLPES"
     gene            complement(21155..22212)
                     /locus_tag="PLJ82_00490"
                     /pseudo
     CDS             complement(21155..22212)
                     /locus_tag="PLJ82_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
     gene            complement(22217..22993)
                     /gene="argB"
                     /locus_tag="PLJ82_00495"
     CDS             complement(22217..22993)
                     /gene="argB"
                     /locus_tag="PLJ82_00495"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOR40021.1"
                     /translation="MKQSLIIIKVGGKIVEESVSLASLLKDFAHTEGYKVLVHGGGRS
                     ATKLSEKLGIESKMVDGRRITDKQTLDVVTMVYGGLVNKNIVAGLQALGVNALGLTGA
                     DMNIIESVKRPIKEVDYGYVGDVKHVNAQIVASLIQQGIVPIVAPLTHDGAGSMLNTN
                     ADTIASELAKSLSTYFDVTLVYCFEKNGVLSDEMDDNSVIPLITPSLFNKYVHQGIIK
                     GGMIPKLENAFQAIQAGVSKVVITNATAIGKDAGTVVLTD"
     gene            23141..23689
                     /locus_tag="PLJ82_00500"
     CDS             23141..23689
                     /locus_tag="PLJ82_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR40022.1"
                     /translation="MQKRKITELNRISVEEFQSKTKLPLVVILDNIRSLHNIGAIFRT
                     CDAFLIEEIYLCGITATPPNAEIHKTALGAENTVKWVYFESIMDAIQQLQTKGYSVFA
                     VEQVKQSIPLNELKIDVTKKYAIIVGNEVKGVQQVAVDMCNGAIEIPQFGTKHSLNVS
                     IAAGIVIWDLVKELSTSQSERG"
     gene            23686..24270
                     /locus_tag="PLJ82_00505"
     CDS             23686..24270
                     /locus_tag="PLJ82_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOR40023.1"
                     /translation="MSLSVCIYCASSNAINSVYYQETEKLARLLAQNNVNVKFGGGSS
                     GLMGKIADVYVEENNDNIVGIIPKFMCEEGWQHQQLKQLIVTETMHERKQKMIENVDA
                     AIALPGGIGTLEEVLEVLTWKQLGIFLKPILLVNINGYFDPLLIALEHAVSENFMRKE
                     HLQLFTIISSVENILQDIQKNPTYDVSIRKIAQI"
     gene            25125..25886
                     /locus_tag="PLJ82_00510"
     CDS             25125..25886
                     /locus_tag="PLJ82_00510"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOR40024.1"
                     /translation="MKIKKILVSQPAPENDKSPYYDIAEKYNVKIEFHPFIKVEPLPL
                     KDFRQQRISILEHTAVIFTARTAIDHFFSICEELRITVPETMKYFCVTETIANYLQKF
                     IDYRKRKVFFPKTGKQEDIIIPVTKHKTEKFLLVVSDVHTDKFVSLLEKNTIQYTKGV
                     FYRTVSNYFSPEEAFDYDMLLFFSPAGVDSLFKNFPNYQQGEVYIGCFGTTTALAVKN
                     AGLRLDVEAPTKESPSMTSALDAFLKEKAKMNGKK"
     gene            25963..26370
                     /gene="rnpA"
                     /locus_tag="PLJ82_00515"
     CDS             25963..26370
                     /gene="rnpA"
                     /locus_tag="PLJ82_00515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOR40025.1"
                     /translation="MKTLRFTFPKKEHLCGKTTIDKLFEKNNSFLQYPLRVLYKIEKN
                     PDNKIPAKILLSVSKKYFKRAIHRNTLKRKMREAYRKQKSPLILLLSEKNLSITMAIL
                     YISKEPISYEQIDAGMKECITKLMHKIENTTPK"
     gene            26370..26591
                     /gene="yidD"
                     /locus_tag="PLJ82_00520"
     CDS             26370..26591
                     /gene="yidD"
                     /locus_tag="PLJ82_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOR40026.1"
                     /translation="MKKLLQTILLIPIYIYRGIISPIKPPSCRYTPTCSEYAIQAIKK
                     HGPLKGMHLAIRRLLSCHPWGGSGYDPVP"
     gene            26608..27252
                     /locus_tag="PLJ82_00525"
     CDS             26608..27252
                     /locus_tag="PLJ82_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR40027.1"
                     /translation="MYYNIHSHKVAKCDNTITIYNVSLHNDITISALSSIGIAPWESD
                     LYTKTNVALLTKYGINENVVAIGEVGLDKRIDTPLTQQLAIFTHQILIAEQCNKPMII
                     HCVGYYNELIQLFRKRRPTVKYILHGFRGKSPLAQELVKHGFYISLGEKSVLHQESIQ
                     QIPLNKILIETDDSDIHVEEVYKSISSIKNISVEELKKSIEENITQCFGWNLRN"
     gene            complement(27257..29077)
                     /locus_tag="PLJ82_00530"
     CDS             complement(27257..29077)
                     /locus_tag="PLJ82_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOR40028.1"
                     /translation="MEYTFFDLLVLIGSLGLFLYGMKLMSEGLQRVAGDSLRSVLAAM
                     TKNRIMGVITGVFVTALIQSSSATTVMVVSFVNAGLLTLFQSITVIMGANIGTTVTAW
                     IISLFGFKVSIAAFAVPLIAIAFPLVFSSNNKRKSLGELILGFALLFMGLDMLKNAVP
                     DLRSNPEILAFLTQYTNFGFGSVLIFLLIGTILTIVVQSSSATMAITLIMCSKGWISF
                     ELGAAMVLGENIGTTITANIAAIPANVSSRRAALAHSLFNIFGVIWMLFVFYNFIDMV
                     ADIVQNYGPGDPRQLQAMTRTIDSETLNLISTADSSSLSVDQLALKEKFEGNQLATSY
                     ALSVFHTAFNIINTFIMIFFVGLIAKTVSWIIPKKKSDEEFHLTHISTGLLSTSELSI
                     IQATKEVNVYAERTLRMFYMVKELYVEKDVQAFQKTYDRIAKYENISDRMEVEIAQYL
                     TKVAEGRLSDESKNRLQSILRMVSEIESIGDSCYNMARIIKRRNEDKSEYTEQMNANV
                     EKMFDSVIKAYDEMMHVLADFDTKTSVDLGYCKEIESEINELRNALKMQNVVDVKGKK
                     YSYQASVTYMDLIIECEKLGDYIINVEEAVKDSTTLTKKI"
     gene            29177..30841
                     /locus_tag="PLJ82_00535"
     CDS             29177..30841
                     /locus_tag="PLJ82_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOR40029.1"
                     /translation="MGNYLEIICKNNQKKALIPFGRSLAEVFEDLKIELPYQVVAARV
                     NNKIESLNYCLYRNKTIEFVDLSCESGMRVYVRSLVFVLFKAIVDLIPNGRLRVEHPV
                     SKGYYCEITANGEKISFEQLAAIKIRMKEIIEQNIRFVSEDKPTSEVIEVFAKHNRTD
                     NVDLLQTYKNYYTEYHSLDGLPDFYSGVVLPSTGYLSLFDIHPYYKGFLIQIPKRSNP
                     IELEEIVVQNKLLEVFADHYKWCRIMGINNIADFNNIEKGKLRDFIKVTEALHEKQIS
                     KMADTIAERSEKTKIVLVSGPSSSGKTTFTKRLSVQLMASGIKPISLSLDNYFVDREN
                     TPLDENGDYDFESLYALDIAYFNKQLAELLNGEEIQLPTFNFETGKRMFKGEKLKLHN
                     DNILIIEGIHALNPTLTSSIPSETMYKVYVSALTTLSLNDHNWIPTTDTRILRRLIRD
                     YKYRGYSAIDTLKRWPSVKKGEEKWIFPYQENADAMFNSSLLFELAALKKQAEPILME
                     VHHDSEAYPEAHRLLKLLQFFEPFPYGEIPATSLLREFLGKSGFRY"
BASE COUNT        10934 a         5709 c         4830 g         9486 t
ORIGIN      
        1 tatctgagtg cataattttg cgtgtttcgt gatacatttt catctcctct ttggtatatc
       61 cattatcggt aaatacatca atttgtggaa taaggttata tgccaattgg taagcaaatt
      121 tggatacagt tggggtttct ccatgtaaca cctgtttgta ttgtaattct aattcatcca
      181 tagctgcagc tcctgcccca ctagccgctt ggtaagtaga aacatgcaca cgtttaacgt
      241 gcgataaatt ttctattgct tgcaatgcaa ctaccatttg tatagtagta caatttggat
      301 ttgcaatgat attgcgtggt ctattttttg catctgcagc attcacttct ggcactacca
      361 aaggcacgtc tgtatccata cgaaatgcac tagaattatc tatcattaca gcaccaaact
      421 ttgtaatatc ggctgcaaat tcatttgaaa tacctgcacc agcagatgta aaggcaatat
      481 ctacaccctt aaaatcgtcg ccatgcttta attcttttac aaccaacttt ttgcctttaa
      541 attcatattc tgatcctgca ctacgtgatg aaccaaacaa tactaattca tccattggga
      601 aattacgctc agcgagtaca cgcaaaaact cttgacctac tgcaccgcta gtaccaacaa
      661 ttgctacttt cattttttac tgttttataa cgtttactta ttaagtattt gcatcctcta
      721 gtcaaaattt ctttttcgac gcaaactgac tacaaaatta cgctttactt tctaattatt
      781 aaacaaataa actaaaaaat agtatttttt atgcttttta gcacaaaaag caacactcat
      841 cgacgccaac aacgcttttt tgatggctaa cattttaaca aacaaaaaaa taatagttac
      901 tttgtcgctc tatttttata gccatgaaaa acattctcat tatttttctt tgtttcctat
      961 cggcaagtac tttggctcaa gtgaatgaaa cattttctga tggtaatttt tcgcatgctc
     1021 ccatttggga aggtaacgca tccaattttg taatcaatag caatgcacaa cttcaactaa
     1081 atgctcctgc agaagcaaat aaatcgtatt tagttacaaa ttcgaatgtt acaaccaatg
     1141 ccgaatggaa aatgtatggc aaaatgatgt ttacaccaag ttctgctaat tatgctcgtt
     1201 ggtacttaat tgctgacaaa caagacatta ctggctcatt gaacggatat tatttgctta
     1261 ttggtaatac gtcaaaaaat atttgtttgt ataagcaaac aggtactacc tccaccaaaa
     1321 tactcgatac acccatcaac cgattaaatg gcactactaa cgaaattaac attcgcatta
     1381 cccgttcaag cgaagggtta tggcaattat ttacacaatt acctacagaa aattcgccta
     1441 ttttagaagg cactacaacg gacaatacgc acaaagctgc tctatatact ggtattgttt
     1501 gctattacac cgcaacacga aatacgggat tcatttttga tgatatcttt atctctggag
     1561 aaacatatga acctccaatt ttgcccagct ataaagcatc gtttggagac attgttttta
     1621 atgaaattat ggcaaatcca aatcctcctt ttggattacc caatgaagaa tatattgaac
     1681 tctacaacaa tacttcggaa gctatcaatt tagaaaacta tgtattaaaa ggattttcaa
     1741 aaacatgcgt tttccctacg tatgtattgg ctcctcatag ctacgttgta gtatgtagca
     1801 ccaaggcttt tcccgcttta gaaatgtatg gtaattgttt ggctctcgac aaatttccaa
     1861 ccctcaccaa tgcaggtgga taccttgaat tattcaatgc agaaaatacc atactttcat
     1921 gggtcgatta ttcggaaaat tggcataccg attctttcaa aaaaaatgga ggttggagtt
     1981 tagaatgtct cgatgcacat aatttaattg ggaacaatac taattggaac tcaagcatca
     2041 attatcaagg aggaactcca aatggagcaa atagtatttc aggtataaac atagatacat
     2101 ccgagttgtc tgttataaac acaagcattg aatcaaacag ttcttttgta ctatatttca
     2161 ataaaccaat gtctgtgaat cttctcacac aaacggatat cattactata tacccaatgc
     2221 gagagataag ttctatcgaa tttttatcca taaaacaaga cgcaatacgc atcacactta
     2281 caaatccact tctattacat gagcaataca cattaaacat tactgacgtt gaagatatta
     2341 ataaaaacaa acattctatc gttagtcgct tagccatacc tgaacttcct tcaaatcaag
     2401 atgttgttat caacgaggtt ttatttaacc caaaatctga agggacggat tatgtcgaaa
     2461 ttgttaatcg ttcgcaaaaa gtaattgatt tgcgagacat catgcttaca aataggaaac
     2521 aagatggaca attagccgtt tttccagtac tgagcgaaaa aggctactta ttgtttcctg
     2581 gtgattattg cctactaagc actagcaaag aaggcgtttg taattattat gaatgccctg
     2641 atgatattca ttttgtaaca gtaccctcat ttccaagcat gcctgatgat aaaggaacta
     2701 tcgtgcttac tactttatca aacatactga ttgatgaatt ttcgtattcc gaaaaaatgc
     2761 accacacctt actctcaaac agagaaggta ttgccctaga aagactccat tatgatatta
     2821 caacacaaga cgcaagcaac tggcattcag cttcttttac ctgtgggtat ggcacaccag
     2881 gttatcagaa ttcacaattt acagcactca tacagcccac cgcttcgttt caaattaccc
     2941 ccaaaagttt ttctcccaat aacgatggaa acgatgacac agcaaccatc tattattcct
     3001 ttgatgaagc tggctatttc gtaactatac gcatatataa ctcgaacgga aattgtgtaa
     3061 gaaatatact gcaaaataac ttatcatcca cagaagaaaa aacatcttgg gatggaacag
     3121 atgataacgg aaagctttta cccattggga tatatattat aaccattgaa gcgtttacac
     3181 ctacaggaaa acgttttaaa tcgaaagaag tcattgtgtt ggcggcaaaa atctaaaggg
     3241 ctcttcaatc aataaaaaaa atagtgtatc tttgtatctt caataaataa taaaaaaaat
     3301 aattatatgg gaaaaaaagc attgttagtc atcctcgatg gatggggcat aggagacaaa
     3361 tcaaaatcag atattatata taacactcct acaccatatt gggataaatt agtagcaact
     3421 tatcctcact ctcaactaca agcctctggc gaaagcgttg ggttaccaga tggtcagatg
     3481 ggaaactctg aagttggaca cttaaacatt ggtgctggtc gtgtagtata tcaagattta
     3541 gtaaaaatca atttagcttg tcgcaacaac accattttgg acaacccagc agtggtaaac
     3601 gcattttcgt atgcaaaaca acataacaaa tctgttcact tcttaggctt agtgtctgat
     3661 gggggtgtac atagctcttt ggagcattta tttaagctat gcgatatagc aaaaacgtat
     3721 gaaattgaca atacatttat tcactgcttt atggacggta gagatactga ccctcgtagt
     3781 ggaaaaggat ttattagcga attggaagct cacacaaaaa aatctgctgg taaaattgca
     3841 agcatcattg gacgctatta tgcaatggac agagacaaac ggtgggagcg tgtaaaagaa
     3901 tcgtacgatt tgcttgtcaa cggtattggc gaaccacaaa cagacatgat tgctgccata
     3961 caaaaatcat atgataatgg tgtaaccgac gaatttatca aaccaattgt aaataaagct
     4021 gtggatggca aaattaaaga aggtgatgta gtaatattct ttaattaccg caatgatagg
     4081 gcaaaagaat tgaccattgt gttaactcaa aaagacatgc ctgaatttgg aatgcaaaca
     4141 ataccaaatt tacactattg tacaatgact ccatacgatg cttcatttaa aggaatgaac
     4201 attttgtttg acaaagacaa tgttaacaat accctaggag aatacctctc tggtctagga
     4261 aaatcccagt tgcacatggc tgagacagaa aaatatgccc atgttacatt cttcttcaac
     4321 ggaggaagag aagagccttt tgcaaaagaa gaacgtatat taatcaattc gcccaaagta
     4381 gccacctacg acttgcaacc cgaaatgagt gcatacgaag taaaagacgc tttagttgct
     4441 gaaataaaaa aacaaaaaca cgattttatc gtagtgaatt acgccaatgg ggatatggtg
     4501 ggacatacgg gcgtgtacaa agctatcgaa acagccgtaa aagcagtaga taaatgctta
     4561 gaagaaacta tcgaaacagc aaaagctaac ggatacgaag ccattattat agctgaccat
     4621 ggcaatgctg acaacgctct aaatgctgat gggtcagaaa atacagcaca ttcgttaaat
     4681 cctgtgcctt ttgtatatat taccgaaaag aaaaacacaa gtgttaagaa cggtatttta
     4741 gcagatgttg ccccttctat attaacaatt atgggcattc cccagccaaa agaaatgaca
     4801 ggaaaaacct taatcgacta aatatcaaaa aaaagagtag gaaaaagaac aaagattttg
     4861 gagtttaaaa aattttatct acttttgcca acccaaatag aagagaaaag gaaataaaaa
     4921 aataataata tgaaaaggac atttcaacca tcaaacaaaa aaagaaaaaa caaacacggt
     4981 tttcgcgaaa gaatggcttc tgctaatggt cgtagagtgt tagcttcaag aagagctaaa
     5041 ggcagaaaaa aactaaccgt atctgacgaa ctttctttga gaaaataaca attattttct
     5101 tgtactatga atatgagaga gtgaaggcac aagcctttac tctcttttcg ttgtattcaa
     5161 aaaaaagcgt atctttgcag tatccaatta ttgaataatt aactgccaat agtgtgtaac
     5221 aatgaaacaa tacagacatg aaacgtaaac ctctaaatat ttttgtaaag cacctactta
     5281 tcatccttgg aacatttatt gcaatcggta tcgtcgcttt cattagctta tcaatatata
     5341 cacagcacgg aaaacaagaa acagttccta atgtaaaaaa catgtctatt acagatgcgg
     5401 aaaaaatact aaactccaac aacctgtcgt atgaaattgt tgattcaata tttaatcgtg
     5461 cattgacacc cggttttgtt gccgaacaag accctattcc ctcatcaatt gtaaagaaaa
     5521 atagaaagat ttacttaact atcaatgcaa aaggagtaca attattaccc ttaccaaatg
     5581 gaataaatat gtcgttaaga caagcaaaat ccttatttga agcagctgat tttgtgataa
     5641 aaaaaattga atataaaaac tcagaataca aagaccttgt gctagacgta aaactaaata
     5701 acagttcaat tcctatagga aaaacaattc cagcaggttc agcccttaca cttgtggtag
     5761 gaaatggttt taatggagaa ttagcaattg taccagatgt aagaaattta tcctacgaaa
     5821 aagcaacgca acttgctaat gaaagtggat ttaatttaga tgaagcaatt tatgacgagg
     5881 taccattaaa taccgccgat gaaaaaaaat acagtatata tcgtcaagaa cctagagcaa
     5941 aagcatcatt agctactgga gaatttataa aagtttggct ttctaaaaca ccccaagaat
     6001 ataatacaga agaagaaatt tcgctagaag aagatatact attttaagct agaatgatag
     6061 aggaattaga agaagattta ctcgaattaa cagatccatc agaatcaccc ctattcgaac
     6121 accatcgttt tgtcagcgat aatggacagg gattggttcg tatagacaag tttttacacg
     6181 atagaatcgt aaatagctct cgtaccaaaa tacaagaagc agccgatgct ggaaatatct
     6241 tagtaaatgg cacagcggta aaagccaatt atcgggttaa acccaaagat gtcataacga
     6301 ttgtaatggc ttatcctcca agagaggtgg aaattatacc tcaaaatatt ccgcttacta
     6361 tcgtatacga agatgatgat ttaatggtaa taaataaacc agcaggaatg gtagtacatc
     6421 ctggacacgg aaattatgat ggcactttag taaacgcact agcgtattat tttagaaaca
     6481 atgcaaatta caatgctact gacccacgat tagggttagt acataggata gacaagaata
     6541 catctgggtt attgctaatt gctaaaaacg aatacgcaaa aataaatctt gccaaacaat
     6601 tttttgataa aacaacagag cgagtttaca aagccctagt atggggtaat tttcaagaaa
     6661 acgaagggac aatcaaaggc aatattggtc gaagcctaaa agatagaatg caaatggatg
     6721 tattcccaaa tggagaacaa ggaaaacacg ctgtaactca ttataaagtt atagaaaatt
     6781 tcaattatgt ttcgtttgtg gaatgccgtt tagaaactgg ccgaacacac caaatacgtg
     6841 ctcatatgaa gtatatcgga catcctcttt ttaacgacga acgttatggt ggaaatagta
     6901 ttctgaaagg aactaccttt aataaatata aacaatttgt acaaaattgt tttgatacct
     6961 gtccgagaca agccctccat gcccaaacac taggatttat acatccaata tctaaggaga
     7021 aacttttttt cacgtcagct attcctgaag acatgaaaga tttactagag aaatggcgaa
     7081 attacacaac cagttatgtt attaattaaa aaaacataca caatgaaaac acgtcaactt
     7141 atactattag cacttagctt attcttatct caattaagtt ttgcatctac ttttaaagta
     7201 aatggaacca tcttagatag caaaaaaaga gggataagca acatggtggt gagcacaact
     7261 caaaccactt caggtgcaat tacaaataac aaaggctatt tctccataca tgcagcaaat
     7321 cctcatgaca cattaataat taacgtacta caagataaaa tactcatccc tataaatgaa
     7381 attaaaaata tcaagattac cattgcagat accataactg cagaagaaat catcgatgaa
     7441 acggtaaata ttggctatca aagaataaaa aaagaagatg taactattaa tcctaatacc
     7501 atctctggcg aaagactttt ggcaactggt gaaaccaact taaatgaagc cctcctaaaa
     7561 gtaatgcctt caataagaca agatcgtaac ggaatgttaa ttatacgtgc tactcaatct
     7621 ttaactcaac aaggacctcc cttgtatgtt gtgaatggga ctccaacaaa taccgtaagt
     7681 aatattcctt taagtgaaat aaaagatgtt caagtattaa aagatggttc tgcttgtgcc
     7741 atttatggag tgcaaggaaa caatggtgta attcttataa atttgaaata agcatttttt
     7801 tttccaattc tattttttac ctttgtgaaa cttaatcata tttactaaat ttagttttaa
     7861 aatggaaaat ctatcaaccc ttacagcatc taagttactt aaaataaacg cattaaaatt
     7921 acaaccaacc aatcccttta catgggcatc aggatggaaa tcacctattt attgtgataa
     7981 tcgtaaaacg ttatcatatc cagatgtacg taattttatt aaacttcaaa tgacacgtat
     8041 tattctagaa aaatatccag atgtacaagc catagcgggg gttgcaactg gagctattgc
     8101 acaaggagca ttggttgctg cggaactaaa cctccctttt gtatatatac gttcttcaaa
     8161 aaaagaccat ggactagaaa atttggtaga aggtgattta caaactggtc aaaaagtagt
     8221 agttatagaa gatttaatat caacaggagg cagtagttta aaagcagtgg atgctattcg
     8281 taataatggt tgcgaagtgt taggaatggt cgccattttt acgtatggtt ttccagtagc
     8341 tcaacaacaa tttagcgaag caaaagtgga attaacaact ttatcaaact atgaagcagt
     8401 cctagctctt gctttagaaa ctaattatat taaagaaagt gatataacca ccctacaaga
     8461 gtggagtaaa agcccatcta cttggaacgc ctaatacaaa cagaatgaca caattcgaca
     8521 gcgaaattaa aaagattaac gctacctcag aaattgtatt taataaactt acaaatctgg
     8581 aaaaccttag tcaacttgag ggctcaatgc cacacgagaa agtaaagatt ctgaaaatta
     8641 cagaaaatac atgtctattt gaagtagaaa tgattggcga aattggcatt aaaatagcca
     8701 acaaagagcc tcatcacctt attttattcg agtctcaaca atctcctttg gcatttaatt
     8761 tatctatata cctaacagaa caaaacgaac ataccgaatt acagattcag ctaaaagcag
     8821 atctacccat gatggtaaaa atgatggtta gtgagcctat taataaattt gtaaacattt
     8881 tagcagatac attaactaga ttatcctatt aaaaatatgg cacaaaaact ttgggaaaaa
     8941 gacacaaaag tcaatagcga aatcgaacgt tttactatcg gcaaagaccg tgaaatggat
     9001 atgtatttag ctaaatatga tgttatagga tctatggctc acatcacaat gctcgagagc
     9061 attggcttac taacacccaa agagctaacc accttactac ttggcttaaa agaaatttat
     9121 aaagaaattg aatctggaca ttttattata gacgaaaacg tggaagacat ccactctcaa
     9181 gtagagttag tgcttacaga taagttgggt gatatgggta aaaaaattca tagtggacgc
     9241 tcacgcaacg accaagttct acttgatttg aagctttttt ctcgagctaa aatacaggaa
     9301 attgcaacag aaactcactt actgttcaat actttattaa cacaaagtga acaatataaa
     9361 agcgttctta tgcctgggta tacacaccta caaatagcta tgccttcgtc atttggacta
     9421 tggtttgggg cttacgcaga aagtttggtt gatgatatgc aactattact agcagcattt
     9481 aaggtatgta atcgcaaccc attgggttct gccgctggat atggttcttc tttccctctt
     9541 aacagacaaa aaactactga tttattggga tttgactcca tgaattacaa tgtagtgtat
     9601 gctcaaatgg gtagaggaaa aatggagcgt acagtatcaa cagcattagc atccattgct
     9661 aacacgatag ccaaattggc tttcgatagt tgtttgttta ccagtcaaaa ttttggattt
     9721 attaaattgg ctgacgaatt tacaacgggg tcgagcatta tgcctcacaa aaaaaaccca
     9781 gatgtatttg agttaacacg tgccaaatgc aataaaatac aaggtattcc acaacaaatt
     9841 aatctaatta ccaacaattt accttcagga tatttccgcg atttgcaaat catcaaagaa
     9901 ttattcatcc cgatgtttga tgagctaata gaatgcatac ggatgactcg ttatatcatc
     9961 gaaaaagtag aaataaacac acacatttta gatgacaaga aatatcaagc catctttagt
    10021 gttgaggaag taaataaaca agtattatct ggtattcctt ttagagatgc ttacaaaaac
    10081 attggtctcg ccatcgaaaa gggagatttt cacccagaca caagcatttc tcatacgcat
    10141 gaaggaagca ttggaaactt atgcaacgaa gaaattaatg ctttaatgaa cgagattata
    10201 tcagcattta atttcaaaag tattcaagag gcagaagcga aattactatt ataaaaccac
    10261 tcattatgtt attcagttca attatatttg gtcctataaa aagcagaaga ctaggtaatt
    10321 cacttggaat caacctgtta cctactgatg caaaaatttg ttcattcgat tgcatttatt
    10381 gtgaatgcgg actaaatgat agtacaaaaa aaggcaatat tcctacacga gaagctgtaa
    10441 aaaatgctct ttctttagaa ctagaaaaac taaaaaaaca agatataaca atagatacta
    10501 ttacttttgc tggcaatggc gaaccaacaa tgcatccaga ttttaaagaa attatagatg
    10561 acacgttgat aatacgtaat caattagctc cagatgctgt tatctctgtt ctttcaaatg
    10621 catggcacat taccaaccca aaagtatatg gggctctgct aaaagtagac aataacatct
    10681 taaaattgga tagtgctata aaagagacaa ttttacaaat aaaccacccc aataatacat
    10741 cctttgaggt atcggaacta attgcaaatt tagcctcttt tcaaggtcaa tgtataatcc
    10801 aaactctctt tgtaaaagga aaccataagg gaaatttgat tgacaacaca accgaagaag
    10861 aaattaatgc atggctaaat gccctcaaaa caattcagcc caaacaaatt atgatttact
    10921 ctattgatag agaaacacct attaaaagct tagaaaaaat acctagttcg acattgaaag
    10981 caattgcctc acaagccaaa aaactgggat ttaatatttc tatttcagta tgaaaacagc
    11041 taagcgaata ctaatctgtc ctcttaattg gggattaggt cacgccactc gctgcattcc
    11101 gataatacat aaattgcaac aagaaggaaa tatactacta attgctagta gtggatgtgc
    11161 aggagaatta cttcaacaag aatttccaac gcttgaatac atacatttcc ctggatttag
    11221 tattacatat agcaaacacc attcacagac tctacgtatt gtattgtcat tcccaaaaat
    11281 tatttattac tctttcaaag aacactttgt attaaaaaaa ctcattaaaa tacatgatat
    11341 taattgcgta atatcagata accgttttgg gctatggaat aaagatatta caacagcata
    11401 cattacccat cagttaaaca ttaaactacc taaacatgtc aaaatctttg aaaaattaac
    11461 ttggtacata catcgatatt ttatcgaaaa atataattat tgtatcattc cagataatca
    11521 acagagtact ttatcattag caggagattt agttcaccaa cgaaaactgc caaaaaatgc
    11581 tttcttttgc tctcctctat cacgcttttc aaaaagtatc cagaatacat ctacaaaatt
    11641 tcaatcaaac attcttatca ttttatctgg aattgaacct caacgttcaa ttttagaaaa
    11701 gctcttattg caacaactta ccaaaataaa ccaaaaagcc atcttgattc aaggattgcc
    11761 aaacgaaact aaatacaaaa aaaacatagg gaatataacg attatatcct atgttttaca
    11821 ccaagaatta caagaattga ttaacaatac agatttggta atttgtcgct cagggtacag
    11881 tagtatcatg gatctagtag ccataaataa acaagctatt ataatcccta cacctggaca
    11941 accagaacaa gaatatttag caaaatatct gcacgagaaa aagttcttta ttgctcaaca
    12001 acaagacaat atagatattt taaaaggaat ggaagagagt aaaagcacaa aaattccaga
    12061 tatgaaattc tatttacagt taccatttta attacaattt ttcgccaaaa gccaaatcac
    12121 cagcatctcc taaacctggt ataatatacg aatgagcatt caattctgga tcaatagccg
    12181 cactccataa agaaatatta ctctcctcag caaaatgatt ggataaataa tcaacagcat
    12241 actgactcgc aataattgtt gcaaagtgca cttccttagg tttccctctg gttaacaagg
    12301 cattatatgc aagttccata gaagaaccac tagcaagcat ggggtctacc aaaataagcg
    12361 ttttaccagt caaatcagga gaagcaatgt attcaataaa aacttcaaac ttcaatgcat
    12421 ccttatattt tctgtatgca gatataaaag cattatccgc attatcgaaa tacgacaaaa
    12481 aaccttgatg aaatggtaaa cctgctctaa atatagtacc tagcacaata tcattatcaa
    12541 cggtatttac gctagctata cccagtggag tagcaatatt tttagttgaa tatgctaatg
    12601 atttactaat ctcataagcc attacctcgc caatacgctc catattccgt ctaaaacgca
    12661 tcctatcttt ttgaacctct tttgaacgta tttgttctaa aaaagtattt agtaaagagt
    12721 cttgcaaagc taaattgtgt gttctcatac tcccatatgt tgatttgcta atttttcttt
    12781 tgctacttca atagaatgca actcaagaag tgcaacatat tgcactgatt ttttatattc
    12841 ttccctccat ccataccatt ggtcttcgtc tgataatgat tcgttatgaa acaatgtaac
    12901 gaactctccg ttatatttct taatcacttc agcataataa ctcataatcc caaacaaatc
    12961 tgtagcttct ttatgcaaat atttttgcaa agtaacatcc attaaaacaa taggatgcag
    13021 acgcatccca gaaatataat ctttggttaa attataaaac aaaaatggag tgcaagtact
    13081 cgctctaaag cctgtatatt ttgcgtatcc taaggaataa tcacgggcaa ttgataatga
    13141 ttcgagatat tcaaagctaa taggaaaagt aaatttcaaa aaatggaatc tatttaaaaa
    13201 aacacgaacc cttgtcatcg actctaagcg ttttttttct tttttcattt gatgtagatt
    13261 tgtactagca aaatatgatg gatgcaaacc aacaatcaac gtacgataaa acaattcctt
    13321 aatgatatta aatggattgt ataatgaata tccatcatat ttacccattg gacctacgtg
    13381 aagaaagaaa ataggacgca gattataatc attgtgcata tcaactatat actcaacatt
    13441 aaaataagga tcaggggctc tattcaaaat aacatttaaa tgatatctaa aatcctcata
    13501 tttcccagtt atgagttttt gaaataaacg aataatatta attgaaagaa acttcgactt
    13561 atatttatac acatgatcaa tatcaataga aggacaaaat cgaaaacgag atttagattt
    13621 tatagtatac atgggaaaat aatccgccaa catctgatct aacaaatatg cccaatgatc
    13681 taccaaggga taatctaaaa attgatgttt atatgccaaa ctattttctg ctttatatct
    13741 tccatgctca tctaagtcaa gactaccata ttcctcgtat cgcgtcagaa gataaaaaga
    13801 tgcggcaaaa atgtcaaatg gtatagaaac atcggctgat tgtgattgaa aaaatatttt
    13861 tgttccattc cattctgtaa cttctggttg aatatcttta attccccttt taaacaataa
    13921 cccttcaggc tcaatgacaa aggattttgg aatacgatgt tctctagagt aacaaataac
    13981 aggaatagta ctatcaatag caacttgctc ctttgtggta aaagatatat ccacatgcaa
    14041 aatttcttta aatatttgct caaaaatata ttttactcta ggagtatatt tatctgtaaa
    14101 gaccaatacc ataggctact atctataaaa tattatggtc agcaaaactg taatacgaat
    14161 ctcccgcaat aataatatgg tctagcacat tcatatcaaa caatttagcc ccttgcttta
    14221 ttttttctgt tatgttttta tcttcttgac ttggtaataa cgctcccgat ggatggttgt
    14281 ggcacaacac gatacctgat gctgcatgtt gtagtgcata cttaaaaata atccttgtgt
    14341 ccactactgc ctgatgaatt cctccaatcg aaatcttcat tttagctact accttgttgg
    14401 ctctagataa aaaaagtacc caaaattctt cttgaattaa atctgcaaga agtggagcaa
    14461 aaatagaggc tacatgctcg cttgatgtaa tagttacaag ctgattagca tcaatccttt
    14521 ttcgccgtct ccccaactct aaggcagcta ctatactaat tgcttttgca tttcccaccc
    14581 ctttagtaga ttttgttaac tcttttacag ataacctact taattgattt aaatcattat
    14641 ttgctaacaa caatattttc ttagctaact caacagcagt ttcattacga gtgcctgacc
    14701 caataagtat agcaattagc tctgcatcag ataaagcagc tatccctttt tctaacaact
    14761 tctccctagg tctatcctct tgcgaccatt ctttaatact caacaacttc gtttccatca
    14821 ttcgacacca aaaatacaga attattaaag taaaaaaaaa tattcattca actatttaca
    14881 tttgtcaata aaaaaatggc tttcggtaat ctacagaaag ccattttata tatagtcaag
    14941 taagtattac tttttataag ctatttactt ttttagccag ttttgatttc aaattacttg
    15001 ctttattttt gtgaataaga ttctttttag ctaatttatc taacatagct acaacttttg
    15061 gaaacaatgc tacagcttct tctttttcac gtagagcacg taattttctt acggcattac
    15121 gagctgtctt agcataatat ctattatgca attttctttt ttcagtttgt ctgattctct
    15181 ttacggatga tttgtgattt gccatctttc aatctattat tataatttgt agcccctagg
    15241 ggagtcgaac ccctctttca agaatgaaaa tcttgcgtcc taaccgatag acgaaggggc
    15301 catcggacaa ttcaaaactt ggacgttttt ctgaattgcg ggtgcaaata tacgggagtt
    15361 ttttaattct gcaaaatttt attgcgattt tttgcacaaa aaacaacttt tataaaaaaa
    15421 gaaacaacgg aacacctcca acacacacca aaccaaccaa aaacccagca aataactgac
    15481 ccaaagaatg tgctttaagt ttcaaccgag aaaatgccac cattccggaa ataataaaaa
    15541 gagcgatata caaccacatt gcattttctt taaaataata cgttgtagta agaaccccgc
    15601 caagcaatcc tcctattcca cccatgtgtg cactaatctt ccaccacata tttacaaaaa
    15661 acacagatac aaccgatact aaaagaccat acaccagata caataaaaat tgaggcactt
    15721 gaagtattgt atatacataa gctaacatcc ccaatgaaat aaccgaaatg agatagggat
    15781 aaaaacgttc ttctcttttg gctatttgag gagaagttac tatacccatt ttatataaaa
    15841 taacagcact aacagcaggc acaaacacag aaaaaaatat agcaataccc aaaacaagcc
    15901 attgcccaat ggctgtatat aaatacaaat aaccagaatc tatgagcaat aatactccgt
    15961 aaataggaat aagcattggt tgaaaaaaaa tcgtaaaaaa ttgtgctatt cgttttgaaa
    16021 catccatatt attcatttac tattgcatta actcattttc agcaccatta tcataccaat
    16081 ctccattatc tttgattagc tgtataagcc gttgtacggc ttctttttca gggatatttc
    16141 tttgtacgca ttcttgtttt ttatacaaac ttatcttccc tttgcctgct cctacgtatc
    16201 cataatcagc atctgccatt tcgccaggac cattcactat acaacccata ataccaattt
    16261 ttagatgatt aagatgcgag gttgctcgtt ttatatgtgc tatggtttgt tgtaaatcaa
    16321 acagggttct accacaacca ggacacgaaa taaattcagt tttactcatc ctagcacgtg
    16381 tggcttgtaa aatagaaaac gataatgaaa cagtatcttg tatcttttgt ttgttttcta
    16441 acatgatacc atcaccaaat ccatcaagaa aaaatgaacc aatatcaaca cttgacttta
    16501 tttgcaacat ttcaatatcc tcttcattat aggcacgttg caacactact ggattctcaa
    16561 cctgataata atgtaacgtt gcaaaaactt ttcttccttc tcctactgga ttttcagaca
    16621 gcaattgaac tattaaaatt acccgtgtag tagcagaaat aaactcgcaa atttcttttg
    16681 taatttctgt cgatttcatc gaaagaaaaa caatatccgc atcacacatt tgtatatttg
    16741 tgtacgtttc tatcgaaaac attggatacg tattttcttc ctttcgccac acagccgcat
    16801 caataatttt tggcgttgga tatgtattaa cgatactcga tcccacataa ataaaatctg
    16861 gttttgtagc agtaacatct ataccattct ctgtatctga taatgaatga atcactattg
    16921 gaacatgcgt atttccaatc ggtcctacag ggattgattt tctgcgatta tacgcaaaag
    16981 gcttaaaaaa gttattgtgg taaataaatg gttctatcgt tggtgctttt gctacttggt
    17041 taatataatc taccatctta tttgccaccg gcaattcatt ctttggatgc tcactaagag
    17101 acaccctaat tgtatcacca ataccatcta taagtaacgc tccaatacca acggcagatt
    17161 ttatacgacc atcctctgca tctccagcct ctgttacccc aagatgcaat ggaaatctca
    17221 ttccttcttg ttgcattgtt gccaccaata atcttacagt atgcaccata accatggtgt
    17281 tcgatgcttt tacagaaata acaatacgta aaaaattatg ttttctacaa atacgcaaaa
    17341 actccataca cgattccacc atcccttgtg gagtatcacc aaaacggctc attattctat
    17401 cactcaacga tccatgattc acgccaatac gaatagcagt agaatgttct ttacaaattt
    17461 ctagtaatgg gatcagtttt tcttcaattt tagcaatctc tttggcatac tcttcatcag
    17521 tatactcata ttgctgaaaa gttttaatac tatccacaaa attacctgga ttaatccgca
    17581 ctttttctac tattcttgca gcttcttcag caactttggg attaaaatga acgtctgcca
    17641 caagtggcac aacatagcct tttgccaaaa ggttcttacg tataaaatat aagttttgag
    17701 cctctcttac gccttgcgtt gttaaacgca ccaactctgc tcctgcatca attaattcct
    17761 cacactgagc agtacttgca tcagtatcca tcgttgatgt attcgtcata gactgtactc
    17821 taatgggata ctttgaacct acccaaacag taccaatgag cacctgatct gtatttctac
    17881 gatgataatt atatatatca agacaataat ctcccatgtg tattttcata aaaattcaac
    17941 gactctttcc attttcatac ttcttgagcc ttttattagt attgataacc cctgaaaatc
    18001 tttttcttgc aaataagcaa ccaattcatc tccattagta agtgtggtaa aatttgattt
    18061 tgtttgagag aataagcttc ccactaaaca gaccttatcg aattgcaaca gatctaataa
    18121 atccacgatt ttttgatgtt cattttgagc ctctttacct aattcaaaca tatctccaag
    18181 tattaacatt ttgggtaaag atgttgttat atttttgaaa ttacgaatag ctgcttccat
    18241 gcttgtcgga tttgcattat acgcatccaa aatcaattca tttttggacg ttttcatata
    18301 ttgcgaacgg ttattttctg gcacatatgc ttcaatacca tccttgcact gtaatgcact
    18361 tacaccaaaa tatactccca cagcaactgc ggctaataaa ttagataaat tatattcacc
    18421 aattaatgct gtttggataa aataggttga atcatcattt tttgtccatt caaacgaaac
    18481 aaaaggagaa acctgtttta ttcttccact cacataattc tcatagttag taccataaga
    18541 aatttgattt gttcctaatg ccatttcttg cagatacgaa ttatctgaat caataaaaat
    18601 tttaccattt gcttttagaa tataatcata taattctttc tttgttttga taacaccttc
    18661 aaacgaacca aatccttcta aatgagccct gccaacattg gtaataattc cataattggg
    18721 caacgctatt tccacaagct cttgaatatc tccagggtga tttgctccca tctcaataat
    18781 agcgaactct acatcaggcg taatgcctaa tatagttaag ggcacaccaa tgtgattatt
    18841 taaattccct ttcgtatagt gcactttgta tttttggcgt aatacagcag cacacaattc
    18901 ttttgtggtg gttttaccat tacttcccgt aattcctaat acaggtatcg ttagtttttt
    18961 tcgatgaaaa cgtgccaaat cctgcaaagt aagtagtaca ttgtctacta caatacaccg
    19021 agcatcatgt atttgcaatg gttcgtcaat aatagcataa gccgctccct tttctagtgc
    19081 ttgcatagca tattgatttc cattaaaatt atcgcctttt aaagcaaaaa aaatggaatt
    19141 acttgggcaa tttctactgt ctgtagttat gtgtggatga gctaaatata actcatataa
    19201 atctgatata ttcataatgg tgtaaatatg taacgcaaaa gtaaaaaaaa gctagcgatt
    19261 aagtggaaaa aagacaaaaa aaaacaaaat caagcaaaaa tttatacctt tgcaactata
    19321 attctcatat atcatagaat gagacttcat gttatatgtt agttaatgca aaattcatct
    19381 atttctctta ttacataaca atgttaattt accatgacaa aacatcttat ttcatttttt
    19441 ttcgctatcc tactaattgg tcttgcatct tgtttaaatg agaaagaaga tttatcaaac
    19501 aatccacatc cgagtgcagt aagtatacaa acaaatacgc ttgccccaga tattgaatca
    19561 acagtgttta cgattgatga aaccaatctt gccataagca atagtgactc aattgcatat
    19621 aatagtagga ttgataaatt ggtacctgta ttttcttttg cagaaggggt agaacaaatt
    19681 ctgataaatg gaactatttg gaatacaaca gattcgatag atttttcttc tcctgttatt
    19741 attcaattta aagcaaaaga taaactaacc acggcagaat atacttttaa ggtaaacaag
    19801 cacacagtag atccagaggc tattacatgg actaaaagag cagacaattt taccaactta
    19861 acaaacatag aagaaatggc aggcttatgg ttccaatcac aagccatact ttttgttcaa
    19921 aaaggaaatg aaacatttgt gttgaaaagc aacaacggac aaacatggac agaggcaata
    19981 tcaagtaata ttccgttgga ggtcagcacc atacttgttc ataaaaatgc catacttgcc
    20041 atttcaaaaa atcatcatct tataacatcg gtagatggga ttaactggag cataataagt
    20101 agtagtgatg agatttcatt tattaacatc ttatgttcat tacaaaacaa agcttggata
    20161 gttggagata aaaacaatat aacatatata tactcatata attcggaatc tgaaaccata
    20221 actgaagtta caactgtttc tgacaaatta ccgtatattg aggtttcaaa ggcaatgatg
    20281 aaaacgaaaa atggtattga aaaatcagta ttgattagtg ggaagaccaa caacgacact
    20341 cctgtatatt cagtattttc atctatgact ggagactatt gggtaaatgt aataacagaa
    20401 acggcatcat taacttttgg agaacgtata cacgctactg ctatttggct agacagcaag
    20461 ctattgttat ttggcggatc aaccgccaac aacaacaatt tgttagatgt atacatttca
    20521 tcagacgaag gctacacttg ggaaatagcc tcatcaaaca aacaactccc tgtggagatt
    20581 ggtgcaagaa aaaagatgat tggttttgtg gatagcaaca acaccctttg gctaattgga
    20641 ggtatagatg ctttgggcaa catacaatct gatgtatggc aaggtagact aaacaaattt
    20701 gattttataa aacaagattg atatgtcaaa aacagctcta tttcctggta cattcaaccc
    20761 ttttacaatt ggacattata gtttggttaa acgtgctcta accatatttg ataaggtaat
    20821 aattgctatt gggattaact caactaaaga gaataatctt gatgaaatag aagaaaataa
    20881 aaaacaaata gccgatctat ttaaaaatga gaatgtaaaa gtaattacct atactaattt
    20941 aaccgcagat atcgtaaaag cagaaaaagc atcttgtatt gttagaggaa ttagaggaat
    21001 aagcgattat gagtacgaaa aaaatatggc tgatattaat aaagagttat ttgaaatcga
    21061 aaccgtattt ttattttctg atttaaaata tgctcacatc tcctcgtctc ttatcagaga
    21121 acttaaacgc tttggcaagg aatataaaca cctattacct gagagttaaa ccatcaatta
    21181 attgttatat aaacctattc cttcttctat ttcagaaata aaaatatatt catctgccgt
    21241 atgagagcgg gctgaatccc ctacaccaat ttttacactt ggaaactgca taaatgtttg
    21301 atcagacaag gtagctgaac caaatattgt tggttttatc gaaagaaatc tctttacaaa
    21361 cacatggttt aaatctatag cagaagaatt aagacgaaaa gatcgtggtg taacatctgc
    21421 ttctacatat tgtttcacaa tgtcgaatac ctgctgattg gaataacact cgttgacacg
    21481 gatatccact gtaaacgcac aagcatcagg cactacatta tgctgtttgc ccgcctgaat
    21541 cattgttaca gacattttta ctgctcccaa taaatcagat tctttttcga attggtaatt
    21601 tttaaaccat tcaatatcgc gaattgcctt ataaatggca tttacgcctt cttcgcgtgc
    21661 agcatgacca ctttgtcctt tggcgacaca atcaagcacc atcagccctt tttctgctac
    21721 cgccaaattc atttgggtag gttctcctac tattgcaaaa tcaatttttg gtaatacggt
    21781 taataagctt tccataccat ttactccaga aatctcctct tcagccgaaa tggctaataa
    21841 taaattatag gattgttctg tgtcttttaa cattaaaaaa acagtgatta atgatactaa
    21901 agatgcatgg gcatcattag ctcctaagcc aataattcgt ccgtcaagca gtgttggctg
    21961 ataaggagga aatgtccacg aatcgctcgg acgcacagta tccatatgcg aatttaacat
    22021 tagcgtgggt ttgttttcat catacgattt agcaaaacaa aagagattgt ttttatggcg
    22081 ttgaacatca caatcatgtt gttttaaaaa attaacaaga aaatcagatt tttcttgttc
    22141 acttttacta attgacggta taacaagtaa tgatgttaat agttcaattg gattaataat
    22201 tattgctttc atatagctaa tctgttagta ctactgttcc agcatctttg cctatggctg
    22261 tagcgttggt tataactact ttactcactc ccgcttgtat ggcttgaaaa gcattttcaa
    22321 gcttgggtat catccctccc ttaattattc cttggtgcac atatttgtta aataatgaag
    22381 gagtaattag cggaataaca ctattatcat ccatttcatc ggataaaaca ccatttttct
    22441 caaaacaata cactaacgtt acatcaaaat aagtggataa tgattttgcc aactcactag
    22501 caatagtatc tgcatttgta tttagcatag aaccagctcc atcatgcgta agaggagcta
    22561 ctatgggtac tataccttgc tgtattaatg aggctactat ctgagcattt acatgcttca
    22621 catctcctac ataaccatag tctacttctt ttattgggcg tttaacactt tctataatat
    22681 tcatatctgc ccccgttagt cctaacgcat ttacccccaa tgcttgtagt cctgctacaa
    22741 tattcttatt aaccagtcca ccataaacca ttgttactac atccaaggtc tgtttgtctg
    22801 taattcttct tccgtctacc attttgcttt ctatacctaa tttctcgctc agtttagtag
    22861 cagagcgtcc acctccatgt accaagactt tatatccttc tgtatgtgca aaatctttca
    22921 aaagagaagc taaactaact gattcctcta caattttacc tcccacttta attataatca
    22981 acgactgttt catgtttcta ttctaataaa tatagtagtt aaaaaaataa gcaagcattc
    23041 atctgtttta ctttcttgct tttttcttgt gcaaatgtaa aaaaaagata tgtttattct
    23101 tatttttacg ctatttttgt gcaaattatt tcatgatacc atgcaaaaaa ggaaaataac
    23161 agagttaaat cgcattagtg tagaagaatt tcagtcgaaa actaaactgc cacttgttgt
    23221 tattttggat aatatacgaa gtctacacaa tattggtgct atttttagaa cttgtgatgc
    23281 ttttttgata gaagagattt atctttgtgg tattacagcc acccctccta atgctgaaat
    23341 tcataaaaca gctttaggtg cagaaaacac ggtgaaatgg gtatattttg aatcaataat
    23401 ggatgcaatt caacaattac agaccaaggg atactccgta tttgccgtag agcaagtaaa
    23461 acaaagcatt cctctcaacg aattgaaaat tgacgtaaca aaaaaatacg ctataattgt
    23521 aggcaacgaa gtaaagggag tgcaacaagt agctgtagat atgtgcaacg gtgctattga
    23581 aataccacag tttggaacaa agcactcact aaacgtatcc attgcagcag gaatagttat
    23641 ttgggatttg gtaaaagaat tatctacatc acaatcagaa agaggatgag cttatcggta
    23701 tgtatatatt gtgcatctag caatgccata aactctgttt attatcaaga gacagaaaaa
    23761 ctagctagat tattggctca aaacaacgtt aacgttaagt ttggaggtgg tagtagcgga
    23821 cttatgggaa aaattgcaga tgtgtatgtg gaagagaaca atgacaatat agtaggaatt
    23881 atccctaaat tcatgtgtga agaaggctgg caacatcaac aactaaaaca gttaattgta
    23941 acagaaacta tgcacgagag aaaacaaaaa atgatagaaa atgtagatgc agcaattgct
    24001 ttaccgggtg ggattggtac gttagaagaa gtgttagagg tactcacttg gaaacaatta
    24061 ggaattttct taaaacctat cttactcgta aacattaatg gatatttcga tccactactt
    24121 attgcgttag aacacgctgt gtcagaaaac tttatgagaa aagaacattt acaattattt
    24181 accatcataa gttcggtaga aaacatattg caggacatac aaaaaaaccc tacatatgat
    24241 gtatccataa gaaaaattgc acaaatttaa ctatctatgc cagaagactc tttagtacag
    24301 attacaaata actatccccc tttgttgcag agccctgact ctattacaaa ccatgttcca
    24361 gtgtgggatg atatactctt ttcttctact atgcacatag atagtacgcg gatactgatt
    24421 cccgaaggaa tggaaagtat atggcgaaca acttcatttt ttcattctga tagtgtgatt
    24481 gcattactat tgagcatatt tattttactg tgtattacta ttgcattttt tcgtaaagcc
    24541 aaaacacatt attttacaag tatatttaaa cacaaaaacg aaacggaaca aattaatcta
    24601 acagtattag aacaatggaa acaagttctg tatagtctat ttggattaat tggatacacg
    24661 agcatagcac tttatttagt tgtagaaaaa tttactatca caagctatta tgaaacatgg
    24721 gaaataggag gatggctttt tgttgccctt tttctgtatt tttttatcaa atatatcttg
    24781 ttgaaactct atgtgtatac atttttttca acaacaaaca gtctcgctat aaggcaatat
    24841 ttttcaatca ttttctatag tggacaactt acctttctgt ttcttttggg aattttattt
    24901 agtaatgaat acgtacacat atacctatat tacggggtag ccatactagc cttattaacg
    24961 ttattgtcaa tgttttatat catatacaga acttttttca gtaaaataca tttagtttta
    25021 tattttattt tgtacctttg caccctggaa attttaccct tatgtttact tataaagttt
    25081 tttgtagggg acatatattt tatcaaccat taaattgtaa agcattgaaa atcaagaaaa
    25141 tactcgtatc tcagccagct cctgaaaacg ataaatctcc gtattatgat atagcagaaa
    25201 aatacaatgt aaagatagaa tttcatccat tcattaaagt ggaacctctg cctttaaaag
    25261 atttccgaca acagcgcata tctattttag aacatactgc tgttattttt actgctcgca
    25321 ctgctatcga ccattttttt tcgatatgtg aagaactgcg tattaccgtg cccgaaacaa
    25381 tgaaatattt ttgcgtaacc gaaacaatag caaattacct acaaaaattt attgattacc
    25441 gtaaacgtaa agtttttttt ccaaaaacag gaaaacaaga agacattata atacccgtta
    25501 caaaacataa aacggaaaag tttttgttag ttgtatctga tgtacatact gataaatttg
    25561 tgtctctatt agaaaaaaat acaattcagt acacaaaagg agtattctat cgtacggtga
    25621 gcaattattt ttcgcccgaa gaggcttttg actatgatat gttgctattt tttagtcctg
    25681 caggagttga ctctttgttc aaaaatttcc ctaattatca acaaggagaa gtgtatattg
    25741 gatgttttgg cacaaccaca gccctagctg taaaaaatgc aggattacga ttagatgtag
    25801 aagcacctac aaaagaatct ccctcaatga caagtgcttt ggacgctttc ttaaaagaaa
    25861 aagcaaaaat gaatggaaaa aaataatacc attttgaagt aatagtatct ctcattgatt
    25921 agcttgaaaa aaaaagctat ctttgagaga tattttttta gtatgaaaac acttcgtttt
    25981 acctttccca aaaaagagca cttgtgtgga aaaacgacca ttgataaatt gttcgaaaag
    26041 aacaattcat ttttgcagta tcctttacgt gtattataca aaatagaaaa gaatcccgat
    26101 aataagattc ctgcaaaaat cttactatca gtatccaaaa agtattttaa aagagcaata
    26161 catagaaata cactaaaaag aaaaatgcga gaagcttatc gcaaacaaaa aagcccttta
    26221 atactgcttt tatctgaaaa aaatctatct attacaatgg ctattctata catttctaaa
    26281 gaacctataa gctatgagca aatagatgca gggatgaaag agtgtatcac aaaactcatg
    26341 cacaaaattg agaatacaac accaaaatga taaaaaaact attacaaacc atactgctaa
    26401 taccgatata catctacaga ggcatcattt ctcctatcaa acctccgtca tgtaggtata
    26461 cccctacctg ctctgaatat gctatacaag caataaaaaa acatggacct ttgaaaggaa
    26521 tgcatttagc aattagacgt ttattaagct gtcacccgtg gggaggtagc ggatatgatc
    26581 ctgttccata attctaatac gaagaaaatg tattacaata tccactccca taaggtagca
    26641 aaatgcgata ataccattac catatataat gtttctctac ataatgatat tactatctcc
    26701 gcattgtcat ctattggtat tgctccttgg gaaagcgatt tatacactaa gacaaatgta
    26761 gcattgttaa ctaaatatgg tataaacgaa aacgtggttg ccattgggga agtgggttta
    26821 gataaacgaa tagacacacc tttaacacaa caattagcta tcttcacaca ccaaatttta
    26881 atagcagagc aatgcaataa accaatgata atacactgtg taggttacta taacgaatta
    26941 atacaattat ttcggaaacg ccgacctaca gttaaatata tcctacatgg atttagagga
    27001 aaatctccat tagcacaaga acttgttaaa catggatttt acatttcatt gggagaaaaa
    27061 agcgtacttc accaagagtc tattcaacaa atacctctga acaaaatatt gatagaaaca
    27121 gatgattctg acatacatgt agaagaggtt tacaaatcta tttctagtat aaaaaacatt
    27181 tcagtagaag agctaaaaaa aagcattgag gaaaatatca ctcaatgctt tggatggaat
    27241 ttgcgaaatt aagacattat attttctttg ttaatgttgt ggagtctttt accgcttctt
    27301 caacattgat aatataatca cccaattttt cacattctat aatcaaatcc atatacgtta
    27361 cactcgcttg atatgaatat ttttttcctt ttacatccac aacattttgc attttcaggg
    27421 catttcttaa ttcatttatt tctgattcaa tctctttaca atagcccaaa tcaacagatg
    27481 tttttgtatc aaaatcagca agcacgtgca tcatctcatc atacgcttta ataacagaat
    27541 caaacatctt ttctacattc gcattcattt gctcagtata ctccgattta tcttcatttc
    27601 tgcgttttat aatacgtgcc atattataac aactatcccc aatgctttct atttcagaca
    27661 ccatacgtaa aatactttgc aaacggtttt tactctcatc actcaatctg ccttctgcca
    27721 ccttggttaa atattgagca atctcaacct ccattctatc gctaatattt tcatacttag
    27781 caattctatc ataggttttt tgaaaagcct gcacatcttt ttctacatac aattctttca
    27841 ccatataaaa catacgcaac gtccgttcgg catacacatt tacctcttta gttgcttgaa
    27901 taatagacaa ttcggaagta gaaagcaaac ctgtagatat atgtgtaaga tggaattctt
    27961 cgtctgattt ctttttagga attatccaag ataccgtttt agcaataaga cctacaaaaa
    28021 atatcataat aaaagtatta ataatattaa atgcagtatg aaacacagat aatgcatagg
    28081 atgttgccaa ttgatttcct tcaaattttt cttttaatgc caattgatct acagaaagag
    28141 aggacgaatc ggctgtacta atcagattta acgtttcaga atcaatcgtt ctagtcatag
    28201 cttgcaattg tctagggtcg ccaggtccat aattttgaac tatatctgct accatatcaa
    28261 taaaattata aaacacgaac agcatccaaa taacaccaaa tatattaaat aatgagtgag
    28321 ccaaagctgc tcgtcttgaa gaaacgttag caggtatagc ggcaatattt gcagtaatgg
    28381 tagtcccgat attttcgcct aataccatcg cagctcccaa ttcaaatgat atccaacctt
    28441 tactacacat aatcagcgta attgccatag tagcagaaga agactgcact acgatagtta
    28501 atattgttcc tatgagcaag aatatcaaca cagaaccaaa cccaaagttg gtatattggg
    28561 ttaagaacgc caaaatttct ggattacttc gtaaatcagg aacggcattt ttcaacatat
    28621 ccaagcccat aaacaaaagg gcaaaaccca agatcagctc tcctaaggat tttcttttgt
    28681 tattagaaga aaaaacgagt gggaatgcaa tagcaataag cggaacggca aatgcagcaa
    28741 tactaacctt aaaaccaaac aaagaaatta tccaagcagt aacagttgta cctatattgg
    28801 ctcccatgat aacggtgatt gattggaaaa gcgttaataa tcctgcgtta acaaaactta
    28861 caaccatcac tgtagttgca gaagacgact ggattaaagc tgtaacaaaa acgcctgtga
    28921 taacccccat gatacggttt ttggtcattg ctgccaatac tgaacgtaag ctatcgcctg
    28981 ctactcgttg caaaccttca ctcatcagct tcattccata caaaaacaaa cccaatgagc
    29041 caattaatac taataaatca aaaaatgtgt attccataac ggattttatt taaaaaagta
    29101 aggtacaaaa ataatatttt ttatacattt acaaagattt ttttaaataa aagttaaata
    29161 aatatagcat acagttatgg gaaactacct tgagattatc tgtaagaaca accaaaagaa
    29221 agctttaatt ccttttggta gaagcttagc agaagtattt gaagatttaa aaattgaact
    29281 accttatcaa gtggtagccg ctcgagtaaa taataaaatt gaaagcttaa attattgcct
    29341 ttatcgcaat aaaactatcg aatttgtaga tttgagttgc gaatcgggca tgcgtgtata
    29401 tgtacgttcg ttggtgtttg tattgtttaa agccattgta gacttaattc ccaatgggag
    29461 gcttcgggta gaacatccag tatcgaaggg atattattgc gaaattacgg ctaatggaga
    29521 aaaaatctct tttgaacaat tagctgctat taaaatacgt atgaaagaaa tcattgagca
    29581 aaacatccga tttgtatcag aagacaaacc aacatcggag gtaattgaag tttttgcaaa
    29641 acacaacaga acagataatg tcgatttgtt gcaaacatat aaaaactatt acacagagta
    29701 ccattcgctt gatggattac ccgattttta cagtggcgta gtattaccca gcactggtta
    29761 tttatctcta tttgatatac atccgtatta taaaggcttt ttgatacaaa tacccaaacg
    29821 tagtaaccca atagaacttg aagagattgt ggtgcaaaat aaattactag aagtttttgc
    29881 tgatcattat aaatggtgca gaataatggg catcaataat atagctgatt tcaataacat
    29941 agaaaaaggc aagctacgtg attttattaa agtaactgaa gctttacacg agaagcaaat
    30001 ttcaaaaatg gcagatacta tcgccgaacg gagtgaaaaa acaaaaattg tattggtttc
    30061 aggtccttct tcttctggta aaactacctt taccaaacga ttgagtgtgc aacttatggc
    30121 gtcgggtatt aaaccaattt ctttatcatt agacaattat tttgtagata gagaaaatac
    30181 cccattagac gaaaatggcg attatgattt tgaatctttg tatgcactcg acatcgctta
    30241 ttttaacaaa caattagcag agctattgaa cggggaagaa attcaactac ctacgtttaa
    30301 ctttgaaaca gggaaacgga tgttcaaagg cgaaaaactt aaactacaca atgacaatat
    30361 actcattata gaagggatac atgctcttaa ccctacacta acatcctcta taccaagtga
    30421 aaccatgtat aaagtatatg tttcggcact taccacctta tcactcaacg accataactg
    30481 gatacctact accgacacac gtatactaag acgtttgata cgcgattata aataccgtgg
    30541 ctattcggct atcgacacac tcaaacgttg gcccagtgta aaaaaagggg aagagaaatg
    30601 gattttcccc taccaagaaa atgctgacgc catgttcaac tcatcgctat tgtttgaatt
    30661 ggctgccctt aaaaaacaag ccgaacccat tttaatggaa gtgcatcacg attcagaagc
    30721 atacccagaa gctcatcgtt tactaaaact cttacaattt tttgaaccat ttccgtatgg
    30781 agaaattcct gctacatcgc tgttacgaga atttttgggg aaaagtggct ttcggtatta
    30841 agaaaaatgg ggaaattttt atttccccat tttttagtat attcaatatg tatattacac
    30901 cacctctatc ccttgttgag cacacatctc aagagccatg tctttcaatt tgtattttt
//
LOCUS       DAOSRP010000004        29435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1738_length_29435_cov_6.810619, whole genome shotgun sequence.
ACCESSION   DAOSRP010000004 DAOSRP010000000
VERSION     DAOSRP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29435
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_29435_cov_6.810619"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..269)
                     /gene="rpsQ"
                     /locus_tag="PLJ82_00540"
     CDS             complement(18..269)
                     /gene="rpsQ"
                     /locus_tag="PLJ82_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOR40030.1"
                     /translation="MRNLRKERQGVVFSNKMDKTITVAIKWKEKHPIYGKFVNKTKKY
                     HAHDEKNECTIGDTVKIMETRPLSKLKRWRLVEIIERAK"
     gene            complement(282..479)
                     /gene="rpmC"
                     /locus_tag="PLJ82_00545"
     CDS             complement(282..479)
                     /gene="rpmC"
                     /locus_tag="PLJ82_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR40031.1"
                     /translation="MKIAEVKELSNQEIIERIDGEKEKLSQLKLNHSISPLDNPNQIK
                     EVRKTIARLNTELRAREMKQN"
     gene            complement(486..920)
                     /gene="rplP"
                     /locus_tag="PLJ82_00550"
     CDS             complement(486..920)
                     /gene="rplP"
                     /locus_tag="PLJ82_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOR40032.1"
                     /translation="MLQPKKTKFRRQQKGRMKGNAQRGNQLAFGSFGIKSLESKWITG
                     RQIEAARIAVTRYMQRQGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAPITPGRV
                     IIEVEGVPFEIAKEALRLAAQKLPVTTKFVTRRDYDYESPNA"
     gene            complement(949..1686)
                     /gene="rpsC"
                     /locus_tag="PLJ82_00555"
     CDS             complement(949..1686)
                     /gene="rpsC"
                     /locus_tag="PLJ82_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR40033.1"
                     /translation="MGQKVNPISNRLGIIKGWDSNWYGGNNYGDTILEDFKLRKYLEA
                     RLAKASVSRIIIERTLKLITITVCTARPGIIIGKGGQEVDKLKEELKKITDKDVQINI
                     FEIKKPEVDAVIVANNVARQIEGKIAYRRAVKMAIASTMRAGAEGIKIMVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYAHAEALTKVGIIGVKVWICRGEIYGKKDLTPQFAT
                     QKEGARPQFGNANRSENAGKGFKKRKK"
     gene            complement(1693..2103)
                     /gene="rplV"
                     /locus_tag="PLJ82_00560"
     CDS             complement(1693..2103)
                     /gene="rplV"
                     /locus_tag="PLJ82_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR40034.1"
                     /translation="MGARKKISAEENKEALKGLYFAKLNNVPTSPRKMRLVADMIRGV
                     EVHKALGLLKFSTKEAAGRLEKLLRSAISNWEQKTEQKAEDANLIVSRIHVDSGRMLK
                     RLRPAPQGRGYRIRKRSNHVTIYVDTVNTNDTKN"
     gene            complement(2143..2412)
                     /gene="rpsS"
                     /locus_tag="PLJ82_00565"
     CDS             complement(2143..2412)
                     /gene="rpsS"
                     /locus_tag="PLJ82_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR40035.1"
                     /translation="MSRSLKKGPFINAKLDKKVTALNEANKKNVIKTWSRASMISPDF
                     VGHTIAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFRGHGGNKKK"
     gene            complement(2442..3266)
                     /gene="rplB"
                     /locus_tag="PLJ82_00570"
     CDS             complement(2442..3266)
                     /gene="rplB"
                     /locus_tag="PLJ82_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR40036.1"
                     /translation="MAVRKLNPITPGQRHKIIGTFDTITASVPEKSLVFGTSKSGGRN
                     NQGKMTMRYLGGGSKRKYRVIDFKREKDGVPAVVKTIEYDPNRSARIALLFYADGEKR
                     YIIAPNGLEVGQTVLSGVDAAPEIGNALPLQNIPLGTIVHNIELNPGQGAALVRSAGT
                     FAQLTSREGKYAVVKLPSGETRMILCTCKATIGTVGNSDHALERSGKAGRSRWLGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRAPKKASSKYIIERKKK"
     gene            complement(3276..3566)
                     /gene="rplW"
                     /locus_tag="PLJ82_00575"
     CDS             complement(3276..3566)
                     /gene="rplW"
                     /locus_tag="PLJ82_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR40037.1"
                     /translation="MEIIIKPIVTEKATKLTEKLNRYAFKVNRAANKIQISQAIEKNY
                     NVTVEAVNTMNYTGKAKSRFTKSGVITGKTSDFKKAVVTLKEGDTIDYYSNI"
     gene            complement(3578..4207)
                     /gene="rplD"
                     /locus_tag="PLJ82_00580"
     CDS             complement(3578..4207)
                     /gene="rplD"
                     /locus_tag="PLJ82_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR40038.1"
                     /translation="MELSIYNIDGQDTGRVISLDDTIFGIEPNDHAIYLDVKQHMANK
                     RQGTHKAKGRSEVAGSTRKLGKQKGGGGARPGDVKSPVRVGGGRIFGPTPRDYSFKIN
                     KKVKQLARKSALAYKVKNNQLIVLEDFSFDTAKTKAYIALTKNLKVNEKKTLLVLPEP
                     NKSVYLSARNLEKSKVIILSELNTYKILDAQYLMLTEKSVANLVQNFNA"
     gene            complement(4207..4824)
                     /gene="rplC"
                     /locus_tag="PLJ82_00585"
     CDS             complement(4207..4824)
                     /gene="rplC"
                     /locus_tag="PLJ82_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR40039.1"
                     /translation="MPGLIGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKTLENDG
                     YEAVQVGFSEKKEKATNKPEKGHFAKANVTPQRHLVEFKGLGNDVKLGDVFTVDMFNG
                     TEWADVIGTSKGKGFQGVVKRHNFGGVGQSTHGQHNRLRAPGAIGACSYPAKVFKGMR
                     MAGRMGGNRVTVQNLEILKVIPENNLILVKGSVPGAKGSIVIVNK"
     gene            complement(4874..5179)
                     /gene="rpsJ"
                     /locus_tag="PLJ82_00590"
     CDS             complement(4874..5179)
                     /gene="rpsJ"
                     /locus_tag="PLJ82_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR40040.1"
                     /translation="MSQKIRIKLKSYDYSLVDKSAEKIVKAVKATGAIVSGPIPLPTH
                     KRIFTVNRATFVNKKSREQFQLCAYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(5256..7373)
                     /gene="fusA"
                     /locus_tag="PLJ82_00595"
     CDS             complement(5256..7373)
                     /gene="fusA"
                     /locus_tag="PLJ82_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR40041.1"
                     /translation="MAKGDLSYNRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTSWDYQNEKYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVAAFCAVGGVEPQSETVWRQADKYKVPRIGYVNKMDRSGADFFDVVRQVKEVLGA
                     TPCPIQIPIGAEEKFQGVVDLVKMKAIFWHDETMGAQYSIEEIPANLLSEAKEWREKM
                     LEVVAEFDDVLMEKFFDDPSTITEDEIKAAIRKGTISMQINPMICGSSFKNKGVQTML
                     DAVCAYLPSPLDTPEIIGQDVNDAKKQVVRHPDAKEPLCALAFKIATDPYIGRLCFFR
                     VYSGKIDAGSYVYNTRSGKKERISRIFQMHSNKQNPVDVISAGDIGAGVGFKDIRTGD
                     TLCDENNQMTLESMDFPAPVIGIAIEPKTQKDIDKLGNGLSKLAEEDPTFTVKTDEQS
                     GQTIISGMGELHLEIIVERLKREFKVECNQGKPQVSYKEAITQTVELREVYKKQSGGR
                     GKFADMMLKVGPVDDNFEGDLQFIDEVKGGNIPKEFIPSIQKGFKAAMRNGVLAGYPV
                     DKLKVVVVDGSYHTVDSDQLSFEICAQLAFKNACAKAKPVLLEPIMKMEVITPEESMG
                     DVIGDLNKRRGQVEGMDSSRTGARIVKAKVPLAETFGYVTSLRTISSGRATSTMEFSH
                     YAEVSSSIAKAVVTEAKGKVELL"
     gene            complement(7400..7876)
                     /gene="rpsG"
                     /locus_tag="PLJ82_00600"
     CDS             complement(7400..7876)
                     /gene="rpsG"
                     /locus_tag="PLJ82_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR40042.1"
                     /translation="MRKAKPKKRIVLPDPIFGDVMVTRFVNHLMYDGKKTTAFTIFYT
                     ALENVKGKLPNEEKSSIDIWKKALENITPQVEVKSRRIGGATFQVPTEIRPERKESIS
                     MKNLILYSRKRGGKTMADKLAAEIVDAFNLQGASFKRKEDMHRMAEANRAFAHFRF"
     gene            complement(8037..8453)
                     /gene="rpsL"
                     /locus_tag="PLJ82_00605"
     CDS             complement(8037..8453)
                     /gene="rpsL"
                     /locus_tag="PLJ82_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR40043.1"
                     /translation="MPTIQQLVRKGRAKQIDTSKSPALDSCPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNSYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRKQRRSKYGAKRPKPGQPAAAPAKGGKKK"
     gene            complement(8611..8922)
                     /locus_tag="PLJ82_00610"
     CDS             complement(8611..8922)
                     /locus_tag="PLJ82_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOR40044.1"
                     /translation="MENSNNGFSIEIADDIAEGVYSNLAIIAHSTSEFVIDFARLMPN
                     TPKAKVKSRIILNAEHAKRLLLALQENITKFEKQNGKIILSEQKNPPIPMNFGGMKGE
                     A"
     gene            9141..11240
                     /locus_tag="PLJ82_00615"
     CDS             9141..11240
                     /locus_tag="PLJ82_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR40045.1"
                     /translation="MGANEGLSKKAKIFNEIIKDLQYILAIGIIVVLLPSDLQYKYQY
                     TEGKPWQYDLLTATFDFPIYKTEVEISNQRNDIIKTHKPYYVLDTSLKSKQIAFLELI
                     VQSLFAKDTISPISTHVISELKKSLINLYDTGILTSQDQSALLAKEAKYIVIMQNNLG
                     QTRRVEDIITEKEAIRLFLKDASRILPESTLNKLPINELIIGNLVYDAETTKKIKNDE
                     LNNLSLTSGQIQAGERIIDRGEIVTYEKQKILDSLKKQFHQEERKSNNNTLLLGEILI
                     VSCLILLFYLYARLFRKKFFISKRFVSVTLLFIIGFTIATSMIVQHANPKWVYIIPYS
                     LLPIVLSTFFDTRTGLFAHVITVLLSSFMVPLPFEFVLLQITTGMVAIYSLKDLAQRS
                     QLVQSAIIIVVSYCLIYFGYGITIDGDITRINWTMFIYFFVNGVLLLFAYPLIYILEK
                     MFGYISNVTLIELANTNNKVLRNLSELAPGTFQHSMQVSNLASEIAIKVGANPLLART
                     GAMYHDIGKMKNPAFFTENQNKGNNPHQQLSFEDSARIIIQHVEDGVQIAKDANIPNC
                     IIEFIQTHHGTNKVRFFYNSYRLQFPEKEIDDSIFTYPGPNPSTKETAIVMLCDAVEA
                     ASRSLPEYTDEAIDGLVEKIITSLLQEGSLNEAPLTMQHISLTKLILKEKLKNIYHTR
                     ISYPELQKNKLKPNETT"
     gene            11227..13308
                     /locus_tag="PLJ82_00620"
     CDS             11227..13308
                     /locus_tag="PLJ82_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOR40046.1"
                     /translation="MKPLRLFTISLLLLFTGYFVKSQNIGIQKNNPDFLEYLSSEEIQ
                     TGYVPLPFEMPTTPVGTKRSGESFPSQFDSRNVNGTSYILAPRNQVTTGPCWAFAATD
                     VVQASFYKKGYQEGYLSPQLLTNCHNFVYTKTQGGNSLMAVATFARLVGPAFENAAPY
                     DANETACMTTTKEDFPAFVSNVHMINNNIDAVKKAIMDNGSVATKMFYDVAYYNSADK
                     TYFYNGAANENHGISIVGWNDNMVVADKGYGTPSASGVWIVKNTWGTGFGDGGYFYVS
                     YEDTYVGKEVFAFTKRIDKNTIDKVYQYAQHGMTNSVGYTANNPSYGRIKFTTTEREK
                     IAAIGLYTYHPNTEITFWIYEGTDFSKLRYTSEAEVIQYGGYYTIDITAPIYITGDFQ
                     VIVSYKHSTENYAIPIEQNFSNYNNATIQNSGVQWISSSGSSWAPLGADTSYKFNLCI
                     NAYTSIPNPPANDNVQDAVLLNTNTVYENFANAGATVQANEVVPPYNNEEASCYTQNS
                     WCYEGGLQNSVWFKFVATNSVVNIITEGFDNQIALWQSNDDNAENLISGNTSTYQLLA
                     ANDDASVSNAFASLYNVQGLQVGKTYFIQVDGSANGAEGIFSIEIQDIPTSINSTHAS
                     TLLTPEGELTQEIKDKVNYIEVFDVSGRLLQILPKNSSLSNSKLPKQHVLIIKLHYTD
                     GGSDTISWLQN"
     gene            complement(13358..13663)
                     /locus_tag="PLJ82_00625"
     CDS             complement(13358..13663)
                     /locus_tag="PLJ82_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOR40047.1"
                     /translation="MLRHIVMWKVKEEALGKTKLELCTEICHLLQTLPPHITEIKQLS
                     VAINHPLCPADNYDVVLDTVFNSVEDLQTYQVHPKHVEVAGFIKQVVFQRACIDVEC"
     gene            13760..14461
                     /locus_tag="PLJ82_00630"
     CDS             13760..14461
                     /locus_tag="PLJ82_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOR40048.1"
                     /translation="MSIIERLEQERNAAKAQILQGFFKTGKGQYGEGDIFWGISVPTL
                     RGIAKTYYQQIPTEEITQLLHSPIHEQRLLSLYILILRYNHAKDDNEKQAIIDTYLSN
                     TRYINNWDLVDSSAYHLLGKHYVDKPRTILFQLVHSSDLWEQRIAIIATFYFIRQHDY
                     ADTLRMAGVLLHHPHDLIHKAVGWMIREVGNRDYGTAYAFLVEHYAQMPRTMLRYAIE
                     KFDEPIRLAFLKGEV"
     gene            complement(14621..>15133)
                     /locus_tag="PLJ82_00635"
     CDS             complement(14621..>15133)
                     /locus_tag="PLJ82_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOR40049.1"
                     /translation="DGKPISAEEFLNNLFGELPKLFKDEEELRKLWSSPITRRILLEN
                     LDAAGFPKDDLLTLQKLVDMEKSDLYDVLEYVFNGDYIAMTREARAKAAEATIFAFLN
                     DKQKEFISFVLSKYIETGVDELDQDKLPILLTNKYQSLEDAKEILGDVANISRLFIEF
                     QEHLYKQKVA"
     assembly_gap    15134..15233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15234..16720)
                     /locus_tag="PLJ82_00640"
     CDS             complement(<15234..16720)
                     /locus_tag="PLJ82_00640"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOR40050.1"
                     /translation="MNEAETRAELIDPKLKACGWGVVEGSKVLREYHITQGKIQTGGG
                     RGKKEIADYVLVHKGIKLAIVEAKSDELEVGEGVMQAKKYADKLKLETTYSTNGKAIY
                     QICMKSGEEGLVNDFLSPDELWNKTFAEQNDWREKFANVPFEDKSGSWQLRYYQEIAV
                     QRTVEAIANKSERILLTLATGTGKTAIAFQIAWKLFQTRWNLQRDGNRRPRILFLADR
                     NILADQAYNSFSAFPEDALVRIKPTEIKKNGKVPTNGSIFFTIFQTFMSGTDAEGKPA
                     PYFGEYPADYFDFIIIDECHRGGANDESNWRSILEYFTPAVQLGLTATPKRKDNVDTY
                     RYFGEPVYIYSLKEGINDGFLTPFKVKRIKTTLDDYRYTSDDTIVEGEIEEGKLYEEK
                     DFNRTIEIVAREAKRVNIFLEEANQNEKAIIFCANQAHAALIRDLVNQNAKSIDPFYC
                     VRVTANDGEEGERLLREFQDNEKTIPTILTTSQKLSTGVDARNIR"
     gene            complement(16727..17038)
                     /locus_tag="PLJ82_00645"
     CDS             complement(16727..17038)
                     /locus_tag="PLJ82_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40051.1"
                     /translation="MKILKIDNGKGLYFKEPDQWIGIDQIGKEDLLTLLDNAISGDFE
                     MDEFDTAALQNKAHQIIYKHLYQKFNELNENKSRFKDESEQLYKSAIEKYTEAISSEN
                     N"
     gene            complement(17019..19049)
                     /locus_tag="PLJ82_00650"
     CDS             complement(17019..19049)
                     /locus_tag="PLJ82_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40052.1"
                     /translation="MKKIDLHIHTKASPYSDSVYDFSISKLQEYVEKLSIDCIAITNH
                     NLFDLEQFNQISEQIEITVLPGIEIDIEKGHLLLISESTELDDFKTKCNQVEHLIQNK
                     SDYISIAQLKEIFVDLSKYLLVPHYDKDPILKPEIIEQLKPFITSGEVSSAKKFKYCI
                     NDTNSLVPVLFSDLRFTHQMTEFSPRQTFIDIDDKSLRAIKTCLMDKNKVFLHHSEGH
                     RFFQIFENGQKLSTGLNVILGERSSGKTYTLKTISRLYENVKHIKQFELLETDEEKDK
                     KKFDELLTTKQSSVSEQFLKEFKEVVEDVLKIDRKENDKRIESYLTSLLKVAAEEDKM
                     DVYSKCTLFNESKFIETNNETLKQLVEATELLIENAQYRTLIDKHISKKILQELAVDL
                     MKKYEEVQQKNLKVRWVNSLVTNVQKELQSSTASEHVKDIDFYNVLLEKEKLEKFENI
                     AAQIKKEKVIEQKDIRRFKIVASTKRFTGAQELKNKSGIQQTAFSGAFPLYDSPIQFL
                     EELKKMERIPETEFYKYFVDVSYKILNEHDAEVSGGERSEFNLLDRIQHAHHYDFLLI
                     DEPESSFDNLFLKNEVNEQIRQISKTVPVIVVTHNNTVGASIKPDYILYTKKEIVDGK
                     AVYKIFSGNPADTILKTVDGEEINNYNIMLNCLEAGDSAYVERKQSYENLKN"
     gene            complement(19046..20278)
                     /locus_tag="PLJ82_00655"
     CDS             complement(19046..20278)
                     /locus_tag="PLJ82_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOR40053.1"
                     /translation="MNWIKKKLGEVLTIERGGSPRPIDKYLTNSPEGINWIKISDATA
                     SDKYIFETKEKITKEGLHKTRMVNEGDFILSNSMSFGRPYIMKTSGCIHDGWLVLKEN
                     GQKIFDTEFLYYLLSSPYVFNQFDYLAAGSTVRNLNIALVSSVEVPIPPLPEQQRIVS
                     ILDEAFAAIAKAKANAEQNLKNAKELFESYLQEVFENGNENWETKCLDELGTITSSKR
                     IYKSEYVKDGIPFYRTKEIKELANGKNITLELFISRERYNEIKKSFGVPQINDLLMSA
                     VGTIGEIMVIKNKDEFYFKDGNIVWFKGFRNLDTNYLKFALTAFVEKIKSLAIGAAYS
                     ALTIEKLNKYQISFPKSIEEQQTIVHQLDALRAETQKLEAVYQKKIADLEELKKSILQ
                     KAFGGELKTEIKVFAEQV"
     gene            complement(20275..21729)
                     /locus_tag="PLJ82_00660"
     CDS             complement(20275..21729)
                     /locus_tag="PLJ82_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOR40054.1"
                     /translation="MFEQTFKNIDDILHKDAGCGSELDYVEQTSWILFLKYLDDLEKD
                     RATAAELTGKTYTPIIDKDYQWTVWAMPKGKDGKLDHHKALTGDDLTDFVNNKLFPYL
                     KKFKLSAESADTIEYKIGEIFSELKNRIHSGYNLREVINRIDELRFRTHAEKHEMSHL
                     YEDKIKNMGNAGRNGGEYYTPRPLITTIVKVVAPKIGDKIYDGAVGSAGFLCEAFDYL
                     RNSKSLSTKETEILQKRTFYGKEKKSLAYIIGIMNMILHGVEAPNIIHTNTLAENLAD
                     IQEKDRYDVVLANPPFGGKERAEVQQNFPIKTGETASLFLQHFIKILKAGGKAGVVIK
                     NTFLSNTDNATVAIRKTLLENCNLHTVLDLPGGTFTGAGVKTVVLFFEKGKPTQKTWF
                     YSLNLDRNLGKTNPLNENDLAEFVELQKTFADSENSWTVNTKDIDQNTFDLSVKNPNK
                     KEETALRQPQAILEEMKALDKESAEILNSISELI"
     gene            complement(22335..23627)
                     /gene="tyrS"
                     /locus_tag="PLJ82_00665"
     CDS             complement(22335..23627)
                     /gene="tyrS"
                     /locus_tag="PLJ82_00665"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOR40055.1"
                     /translation="MNFVDELEWRGMIHTVMPGTKEQLEKELTSAYVGIDPTADSLHI
                     GHLVGVMMLKHFQRCGHKPIALIGGASGMIGDPSGKSAERNLLDEKTLRHNQDAIKQQ
                     LARFLDFDSDAPNAAEIVNNYDWMKSFTFLDFIRDVGKHITVNYMMAKDSVKKRLSSE
                     SSVGMSFTEFTYQLVQGYDFLYLYDAKNCKLQMGGSDQWGNITTGTELIRRKVGGEAF
                     ALTCPLITKADGGKFGKTESGNVWLDAKYTSPYHFYQFWLNVSDEDAQKYIKIFTFIS
                     KEEIESLVATHLEAPHLRLLQKRLAQEVTCMVHSQEAYEMAVEASAVLFGNATSETLK
                     KIDTATLLAIFEGVPQFEIDTKDLSGEVKLMDLLTTQAPVFASKGEMRKMIQNGGLFI
                     NKEKITDTEAIATSNLLLNNTFILVQKGKKNYYLLRVK"
     gene            complement(23632..24066)
                     /locus_tag="PLJ82_00670"
     CDS             complement(23632..24066)
                     /locus_tag="PLJ82_00670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOR40056.1"
                     /translation="MLDDTYFMKQALAEAKKAFAKGEVPIGAVVVCNQQIIARAHNFT
                     ETLHDVTAHAEMQAITAAASYLGAKYLNDCTLYVTVEPCVMCAGALYWSKVAKVVYGA
                     SEDKYGYTKKVPLALHPKTEVVAGVMADECAQIMKDFFITKR"
     gene            complement(24066..25394)
                     /gene="rmuC"
                     /locus_tag="PLJ82_00675"
     CDS             complement(24066..25394)
                     /gene="rmuC"
                     /locus_tag="PLJ82_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOR40057.1"
                     /translation="MEWMWISIGLVSVLAVLWLIVKYMKQLMHNKLAEQEKALASSIA
                     VLEKEKALAQMQLADVQNVYQQTLQQLSKETEKYQQLSSQYAVLQTQNTHLSERLDTQ
                     KKELTELQQHFTKEFENIANRILKQNSSDFTDVNVKHLTDIVNPLKDKISLFEKKVED
                     AYDKELRDKLSLREEVRKLTELNTRISEEATNLTKALKGDVKKQGNWGEIVLERVLER
                     SGLRKGIEYTREEQIEGDVGQVYRPDVIVHLPDNKHIIVDSKVSLLAYENFVNANDDE
                     KDAYLKAHIVSLRNHIKGLGEKCYQNSPTLNSPDFVLMFLPIEGSFSVALQSDNEIYS
                     FAWDRKIVVVSPTTLLATLRTIASIWKQENQTKNAQEIARLGGALYDKLVGCVEDLNK
                     VKLNIDRASASHNDAMKKLQLGAGNVFVTAEKMKQLGAKTSKQLPEDLQE"
     gene            complement(25407..25622)
                     /locus_tag="PLJ82_00680"
     CDS             complement(25407..25622)
                     /locus_tag="PLJ82_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40058.1"
                     /translation="MKKPSKRNDKNTQKSLLRKNRVTFVLNDKELNALEAYCKRFRVK
                     NKSRFVRTVLMSTVIERLEQSSPTLFD"
     gene            25720..26088
                     /locus_tag="PLJ82_00685"
     CDS             25720..26088
                     /locus_tag="PLJ82_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOR40059.1"
                     /translation="MAIHNKLGEEGEKLATAYLQQKGYRIRHTNWRSGHLELDIVAES
                     HDTLYVVEVKTRSTAYFEHPKDAITNAKIKRIVHATEEYIHRFDIHKDIQFDIMALLP
                     NKHGTFDIEHIPDAFLAPLS"
     gene            26103..26456
                     /locus_tag="PLJ82_00690"
     CDS             26103..26456
                     /locus_tag="PLJ82_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOR40060.1"
                     /translation="MNIEEVRTYCLSKPYVTEDFPFDETVLVFRVAGKIFVLCALDAI
                     PPRINVKCNPQKAIELRELYNYVVPGFHMSKKHWNTIFIESVDNEQQLRTWIDESYEL
                     VIKGLTKKEQKQLNM"
     gene            26557..27273
                     /gene="dapB"
                     /locus_tag="PLJ82_00695"
     CDS             26557..27273
                     /gene="dapB"
                     /locus_tag="PLJ82_00695"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOR40061.1"
                     /translation="MKLALIGYGKMGKEIEQIAISRGHEIVSRIDVDNQEDFKSDAFK
                     SADVAIEFTAPHVAYANYLKAFEAGVSIVSGTTGWLDKINEIKAICHDKQLAFFWASN
                     FSLGVNIFAAVNSYLAKIMNQYSEYNVSMTEVHHTQKLDAPSGTAITLAEGIIKELAR
                     INKWELETASTKDSLAIHAIREGNVPGIHTIRYESEVDSIEITHDAKSRKGFALGAVL
                     AAEFTYGKQGFLGMNDLLQF"
     gene            27289..28716
                     /gene="lepB"
                     /locus_tag="PLJ82_00700"
     CDS             27289..28716
                     /gene="lepB"
                     /locus_tag="PLJ82_00700"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOR40062.1"
                     /translation="MSDIHNEKKTGRFDVSLIQWIKAFVVCLLYVLFIIWVGNFWWLL
                     LLPIFFDIYITKFIPWSFWKKSKNKYVLKIAEWTDAIVFALIAVYFINLFLFQNYKIP
                     TSSLEKSLLVGDFLLVSKVSFGPRVPNTPLSFPLAQHTLPILNTKSYIEYPQWEYKRL
                     KGLDTIKHLDIVVFNFPAGDTVTTKVSNPDYYTLCHDYGREYVHNNKNLFGDVIYRPV
                     DRRENYVKRCVGLPGDTLQIIEHQVYINGVAQEEVEGTQLSYWVTTDGRKISENQFKR
                     IGVSKDDQQFLINNLPNGDNLLDYCGYERNSEGKHNPVYRLPLTKEALNKVKNLPNVI
                     KIEIDNGLISGQVFPFGYKKWTRDNYGPIYIPRKGATIALNLDNLPVYERVIRNYEGN
                     TLEIKDSTIYINAVATTNYTFKMNYYWLMGDNRHNSADSRYWGFVPEDHVVGKPLFVW
                     LSLDKDASLLESIRWERLFKFVHKE"
     gene            28720..29349
                     /locus_tag="PLJ82_00705"
     CDS             28720..29349
                     /locus_tag="PLJ82_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOR40063.1"
                     /translation="MSGVVLTSTYLGNIQFYSKLVHFSEIWIEQHCHYTKQTYRNRCK
                     IATANGVMALSIPTIKPATEKCKTKDIRIDNSQHWQQIHWRALEAGYNSSPFFEYYKD
                     DFAPFYTNKFEFLVDYTFQLQSLVIALLELDCKVQLTDAYTTYDDALDYRDALSAKKE
                     IEDNDFVISSYYQVFENKFGFIPNLSILDLLFNMGNESILVLQKSASKH"
BASE COUNT         8687 a         5510 c         4991 g        10147 t
ORIGIN      
        1 tttcttgttg tatcatgtta ttttgctctt tcgattattt ctactaatct ccatctttta
       61 agcttgctta aaggacgagt ttccataatt tttactgtat caccaattgt acactcattt
      121 ttttcatcat gagcatggta tttttttgtt ttattcacga acttaccgta aataggatgc
      181 ttttctttcc acttgatagc aacagtaatg gttttatcca ttttattgct aaaaaccacg
      241 ccctgtcttt cttttcttaa atttctcata tcaggtcaag tttaattttg tttcatttct
      301 cttgcacgta gttctgtgtt cagacgagca attgttttac ggacttcttt aatttgattt
      361 ggattatcta atggagaaat actatgattc aattttaatt gagataattt ttctttttcg
      421 ccatcaattc tttcgataat ctcttggttg gataattcct ttacttcagc tattttcata
      481 cctcattatg cattaggaga ttcataatca taatcacgtc tagtaacaaa tttggtagtt
      541 actggcaatt tttgtgctgc taagcgtaat gcttctttag caatttcgaa aggaacacct
      601 tccacttcaa taattacacg accaggtgta atcggggcaa caaaaccctc aggggctcct
      661 tttcctttac ccatacgtac ctcagcaggt tttttagtta ttggtttatc tggaaaaata
      721 cgtatccaaa tttgaccttg tctctgcata taacgcgtaa ctgcaatacg agcagcttct
      781 atttgccttc ctgtaatcca tttggattct aaagacttta ttccgaaaga tccgaatgcc
      841 agctgattgc cacgttgggc attacccttc atacggcctt tttgctgtct tctgaacttt
      901 gtttttttag gttgtaacat gtttccttat gtaaaattcg ttaacagatt atttttttct
      961 ttttttaaaa cctttgcctg cgttttcaga tcgattggca tttccaaatt gaggacgagc
     1021 accttctttt tgggttgcaa attgcggtgt caaatctttt ttaccatata tttcgccacg
     1081 acaaatccac actttaacgc caattatacc tacttttgtt aaagcttcag catgtgcata
     1141 gtcaatatct gctctaaaag tatgtaaagg agttctacct tctttataca tttccgaacg
     1201 agccatctct gcaccattta aacgaccaga aaccattatc ttaattccct ctgctcctgc
     1261 acgcatagtt gatgcaattg ccattttaac tgctcttcta taggcgattt taccctctat
     1321 ttgacgagca acattatttg caacaatcac tgcatctacc tctggttttt tgatttcaaa
     1381 aatattgatt tgaacatctt tatcagtaat ttttttaagc tcttctttga gtttatcaac
     1441 ctcttgacca cctttaccaa ttatgatacc aggacgagct gtacatactg taatagtgat
     1501 gagttttagt gtgcgttcaa taatgatacg agaaacacta gccttagcta agcgagcttc
     1561 taaatatttt ctaagtttaa aatcttctaa aattgtatca ccatagttat ttccaccata
     1621 ccaattagaa tcccaacctt tgatgattcc taaacggtta cttattggat ttactttttg
     1681 tcccatctac tattaatttt ttgtatcgtt agtatttaca gtgtctacat aaatggttac
     1741 atggttcgag cgtttacgaa ttctgtatcc tctaccttgc ggagctggac gaagtctttt
     1801 aagcattcta cctgaatcca catgtatgcg actaactatt aagttagcat cttctgcttt
     1861 ttgttctgtt ttttgttccc aattagaaat tgcagagcga agaagttttt ctaaacgacc
     1921 ggcagcttct ttggtggaaa attttaacag tccaagcgct ttgtgcactt ccacgcctct
     1981 tatcatatct gcaactaaac gcattttacg tggagacgta ggcacattat ttagctttgc
     2041 gaaataaaga ccctttaaag cttctttatt ttcttctgct gatatttttt ttctagcacc
     2101 catttttata cttattattt attcctaatt tatattcaac ggttatttct ttttatttcc
     2161 accatgacct ctaaaattac gagttggcga aaattctcct agtttatgtc ctaccatatt
     2221 ttcggtaaca tacactggaa taaatttatt tccgttgtgt acagcaattg tatgaccaac
     2281 aaagtctgga gatatcatag atgctcttga ccatgttttg atcacattct ttttgttagc
     2341 ctcgtttaag gcagtcactt ttttatcaag tttagcgttg ataaatggac ctttttttaa
     2401 tgaacgactc atattattat acttctctta aattaatcaa attacttttt ctttctctcg
     2461 atgatatatt tacttgaagc tttttttgga gcacgtgttt tatacccttt agctaacaaa
     2521 cctttacgtg atcttggatg acctccagaa gcacgacctt caccaccacc cattgggtga
     2581 tccacaggat tcatgacaac gccacgaact cttggtcgcc tacctaacca tcttgaacga
     2641 cctgctttac ctgaacgttc caaagcatga tctgaatttc ctactgttcc gattgtggct
     2701 ttgcatgtgc aaagtatcat acgggtttca ccagaaggca atttaacaac tgcatattta
     2761 ccttctctcg aagtcaactg tgcaaaagtt cccgcagagc gaaccaaagc agctccttgg
     2821 ccaggattca attcaatgtt gtgtacaatt gttccaagag gaatattttg taaaggcaat
     2881 gcattcccaa tttctggtgc agcatccact cctgacaaaa ctgtttgacc tacttctaga
     2941 ccattaggtg cgataatata acgcttttct ccatctgcgt aaaatagcaa tgcgatacgt
     3001 gccgaacgat ttggatcgta ttcaattgtt ttaactacag ctggaacacc gtctttttct
     3061 cttttaaaat caattactct atattttctc ttgcttccgc cacctaagta gcgcatggtc
     3121 attttacctt gattgtttcg accaccagat ttactggtac caaacacaag agatttctct
     3181 ggaacacttg cagtaattgt gtcaaatgtt ccaataattt tatgtctctg ccccggtgtg
     3241 atggggttta atttacgtac agccattttc ttttattaaa tgttgctata ataatctatt
     3301 gtatcacctt cttttaacgt tacaacggct tttttgaagt cggacgtttt tccagtaata
     3361 acaccacttt ttgtaaaacg agattttgct ttccctgtat aattcatggt gttaactgct
     3421 tctacagtta cattataatt tttctcaatc gcttggctaa tttgtatttt attagcagct
     3481 ctattcacct taaaagcata acgattaagt ttttcagtaa gctttgtagc tttttctgtt
     3541 acgatgggtt taataataat ttccatacac ttatctccta tgcgttaaag ttttgaacaa
     3601 gatttgctac tgatttctca gttagcatta aatactgagc atctagaatc ttataagtgt
     3661 ttaattctga aagaattatc acttttgatt tttctagatt acgagccgac aaatatacac
     3721 ttttatttgg ttctggtaaa actaaaagtg tttttttctc attaactttc aaattttttg
     3781 ttagagcgat atatgctttt gtttttgcag tatcaaatga gaagtcttct aatacgatta
     3841 gttgattatt cttcacttta tatgctaaag cagatttacg tgctaactgt ttaacttttt
     3901 tgttaatttt aaagctgtaa tctcgtggag ttggtccaaa aatgcgacct ccaccgacgc
     3961 gaacaggaga tttaacatca ccaggacgag caccaccgcc tcctttttgt ttacctaatt
     4021 tgcgcgtact accagcaact tcacttctgc ctttggcttt gtgtgttcct tggcgtttgt
     4081 tggccatatg ttgttttaca tccaaataaa tggcatgatc atttggctca attccgaaga
     4141 ttgtatcatc taacgaaatt actcttccgg tatcttgacc gtctatatta taaatactta
     4201 gttccattat ttatttacaa ttacgattga accttttgct cctggaactg atcctttcac
     4261 taagataaga ttgttttcag gaatcacttt taatatttct aggttttgaa ccgttacacg
     4321 attaccaccc atacgtccgg ccattctcat acccttgaac actttcgctg gataagaaca
     4381 agccccaata gctcctggtg ctcttaaacg attatgttgt ccgtgcgttg actgacccac
     4441 tccgccgaaa ttatggcgtt ttactactcc ttggaaacct ttcccttttg atgttccaat
     4501 aacatcagcc cattcagtgc cattaaacat atccactgta aagacatctc ctaatttgac
     4561 atcatttcca agtcccttga actcaaccaa gtgtctttgt ggagtaacgt ttgcttttgc
     4621 aaaatgacct ttttcaggtt tgttagtagc tttttctttt ttctcagaaa aacctacctg
     4681 tacagcctca taaccatcat tttcaagcgt tttgatttgc gtaactacac aaggaccagc
     4741 ttcaataaca gtgcatggaa cattttttcc atcggcactg aaaacggaag tcattccgat
     4801 tttttttcct attaatcctg gcatttcaat ttaattaatt attatttact atttatgttt
     4861 gattatctac aaatcaaact ttgatttcta cttctactcc acttggtaac tctaatttca
     4921 ttaaagcatc tactgtctta gacgtagaac tataaatatc aattagtctt ttgtatgcac
     4981 acaattgaaa ttgctctctc gattttttat tcacaaaagt agcacgattc actgtaaaga
     5041 ttcgtttatg agtaggcagt ggaattggac cactcacaat tgctccagta gcttttactg
     5101 cttttacgat tttctctgcc gatttatcga ctaagctgta atcgtatgat ttaagtttga
     5161 ttctaatttt ttgactcatc gtcttttatt ttaattaagt tatttaaaaa aggtattgat
     5221 taagaatcat ttgctaatca acaccttatt atttcttata ataattctac ttttcctttt
     5281 gcttctgtaa ctacggcttt agcgatagaa gatgaaacct cagcataatg agaaaattcc
     5341 attgtagaag ttgctctacc tgatgaaatc gtacgtaaag atgttacata tccaaaagtt
     5401 tcagctaaag gcacttttgc ttttacaata cgagccccag tacggcttga gtccattcct
     5461 tctacttgac cacgacgttt gtttaagtct ccaattacat ctcccatact ttcttctgga
     5521 gtaataactt ccattttcat gattggttca agtaatacag gttttgcttt ggcacacgca
     5581 tttttaaatg ctaactgagc acaaatttcg aaagataatt ggtcagagtc tactgtgtga
     5641 taagaaccat cgacaacaac aacctttaat ttatctactg gatagccagc taacactccg
     5701 ttacgcatag ccgctttgaa tcctttttgg atagaaggga taaattcttt tgggatgttt
     5761 ccacctttta cttcgtcgat aaattgtaaa tctccttcga agttatcgtc aacaggacca
     5821 actttaagca tcatatcagc aaatttacca cgaccacctg attgtttttt atacacttct
     5881 cgtagctcaa cggtttgagt tattgcttct ttataagaca cttgcggttt accttgatta
     5941 cactctactt taaattcacg tttaagacgt tcaacaatga tttctaaatg aagttcaccc
     6001 ataccactaa tgatagtttg acctgattgc tcgtctgttt tcacagtgaa agtaggatct
     6061 tcttcagcca atttagacaa tccatttcct aatttatcta tatctttttg ggttttaggc
     6121 tcaatagcaa taccgattac tggtgcgggg aagtccattg actcaagtgt catttgatta
     6181 ttctcgtcgc acaaggtatc accagtacga atatccttaa aaccaacacc tgcacctata
     6241 tctccagctg agattacatc aacaggattc tgtttgttag agtgcatttg aaaaatacgt
     6301 gaaatacgtt ctttctttcc agaacgtgtg ttgtatacat acgaacctgc atcaatttta
     6361 cctgaataca cgcggaagaa gcacaaacga cctatgtatg ggtcggtagc aattttaaat
     6421 gctaaagcac aaagaggttc ttttgcatca ggatgtctta ctacttgttt tttagcatca
     6481 tttacatctt gtccgataat ttcgggagta tctaatgggc ttggcaagta tgcacaaaca
     6541 gcatcaagca tagtttgaac acctttattt ttgaaagaag acccgcaaat cataggattg
     6601 atttgcatgc ttattgtacc tttgcgaata gctgctttga tttcatcttc agtgatagtg
     6661 ctaggatcat caaagaactt ttccataaga acatcatcaa attcagcaac cacttcgagc
     6721 attttttctc tccattcttt ggcttcgctc aacaaattag cggggatttc ttcaatacta
     6781 tattgagctc ccatcgtttc atcatgccaa aaaattgcct tcattttcac aaggtctact
     6841 acaccttgaa atttctcttc agcaccaatt ggtatttgaa taggacaagg agttgctcct
     6901 aatacctctt taacttgacg aacaacgtcg aagaaatcgg caccagagcg gtccatttta
     6961 tttacataac cgatgcgagg cactttgtat ttatctgctt gacgccatac tgtttctgat
     7021 tgaggttcaa cacctccaac agcacaaaat gctgctacag ctccgtctag gatacgcaac
     7081 gaacgttcaa cctctacggt aaagtcaacg tgtcctgggg tgtcaatcaa atttattttg
     7141 tatttttcat tttgataatc ccaactagtt gtagtagctg cagaagtaat tgtaatacct
     7201 ctttcttgct cttgctccat ccagtccatg gtagcagcac catcgtgtac ttcaccaatt
     7261 ttgtgggtta accctgtata aaatagaata cgctcggaag ttgttgtttt accagcatca
     7321 atgtgagcca tgataccgat attccgattg taacttaaat ctccttttgc cattttagta
     7381 tatcttcaga tgttaaatat taaaaacgaa aatgggcgaa agctctgttt gcttcagcca
     7441 tacgatgcat atcttcttta cgtttgaaag aagcaccttg cagattgaat gcatctacaa
     7501 tttcggcagc taatttatca gccatcgttt ttcctcctct cttgcgagaa tagagtatta
     7561 agtttttcat tgaaatggat tcttttcttt ctggacggat ttcagtagga acttgaaatg
     7621 tagctccacc gatacggcga gatttcactt ctacttgagg ggtgatgttt tctaaagctt
     7681 ttttccagat atcaatagac gacttttctt cgttaggtaa tttaccttta acgttttcta
     7741 aagctgtata aaagatagta aatgcggtgg tttttttacc atcatacatt aagtggttaa
     7801 caaaccttgt aaccattaca tctccgaaaa ttggatcagg taatacgatc cgtttttttg
     7861 gttttgcttt tctcattttt tttaaaattt ttccgttttg tatttggttg ccattgtgtc
     7921 ttcaaaaaat tacgcttaat ttttttactc aaccgataaa atgtctaaat ccaaaatatg
     7981 caaaacagta gtaattaact gttgtttgtt tttcttttaa ttttcacacc gaaggattat
     8041 ttctttttac ctcctttagc tggtgcagca gctggttgac ctggttttgg gcgtttagct
     8101 ccgtatttag aacgtctttg tttgcgacca ttaacgccag cagtatctaa tgttccacgt
     8161 acaatgtgat aacgaacccc tggtaaatct ttaacacgac caccacgaat tagcacaatt
     8221 gagtgttctt gcaagttgtg tccttcacct ggaatatatg agtttacttc cttttggtta
     8281 gttagacgaa ctcttgctac tttacgtaaa gccgagttag gttttttggg agttgttgta
     8341 tacaccctta cgcacacacc acgacgttga ggacaagagt ccaacgctgg agacttgctt
     8401 gtgtcgatct gtttagccct tccttttctt actaattgct gaattgtagg catttttctt
     8461 gtttttgtta taaattatta gacttctttt tgaacccatt ttttgtaaat tcgggttgca
     8521 aagatacaca aacttttgta ctaaacaata ttttagtata aaaaaataca aatttatttg
     8581 agaaacaatt atttaataga agcatacgaa ctatgcttct cctttcatcc caccaaaatt
     8641 catgggaata gggggatttt tttgttctga aaggattatt ttcccgtttt gtttttcaaa
     8701 tttagtaata ttttcttgta atgctagtaa aagtctttta gcatgctcgg catttaagat
     8761 aatgcgagat ttcactttag ctttgggggt attgggcatt aatcgagcaa agtctattac
     8821 aaattcgctg gttgaatgtg ctataattgc taaatttgaa tacacacctt ctgcaatgtc
     8881 gtctgcaatt tctatactaa aaccattgtt tgaattttcc attctatttt gttttaaaat
     8941 tatgtaatta agaaagatgt tttttctaaa aaaaccttat gtttctacta ttttgtatca
     9001 attttgacac attttgtatg gtttcatgtt aattaacaga caaaagtacg atttttaaat
     9061 taaatcattg aattctcaat taaaatattt acatttgctt gttaattttg tagtattttc
     9121 tctgaaaaga agaaagcgtt atgggagcaa atgaaggtct gagtaaaaag gcaaaaatat
     9181 ttaatgaaat tattaaggat ctccagtata ttttggcgat aggtattatt gtagtattac
     9241 ttcccagcga tttgcaatac aaatatcaat atactgaagg caagccttgg cagtatgatt
     9301 tacttacggc tacatttgat ttcccaattt ataaaacaga agttgaaatc agtaatcagc
     9361 gtaatgatat aataaaaacg cacaaacctt attatgtact cgatacaagc cttaaaagca
     9421 agcaaattgc attcttagaa ttaatagtac aatcgttatt cgcaaaagac acaatttcgc
     9481 caatttctac ccatgtaatt tctgaactaa aaaaatcgtt aataaactta tatgatactg
     9541 gaattttaac atcacaagat caatctgcat tacttgctaa agaagctaaa tacattgtga
     9601 ttatgcaaaa taatctaggt caaacacgga gagtagaaga cattatcaca gaaaaagagg
     9661 ctatcagatt atttttaaaa gatgcaagtc gtatacttcc agagtctaca cttaataaac
     9721 tacctattaa tgaattaata ataggcaatt tggtgtacga tgctgaaacg acaaaaaaaa
     9781 taaaaaatga tgaattgaat aatttatcgc tcacttcggg ccaaatacaa gctggagaac
     9841 gtattattga taggggagaa attgtaactt atgagaaaca aaagatctta gactccttga
     9901 aaaaacaatt tcaccaagaa gagagaaaat cgaataataa tacacttctt ttaggagaga
     9961 ttttgattgt aagctgttta attttattgt tttaccttta tgctcgttta tttaggaaga
    10021 agttttttat cagcaaacgt tttgttagtg ttacgttatt attcataatt gggttcacta
    10081 tagcaacttc gatgattgtg caacatgcta atcctaaatg ggtatatatt atcccatatt
    10141 cgttgctacc tatcgtgtta agcacctttt ttgatacccg tacaggatta tttgcacatg
    10201 ttataactgt attgctctct tcatttatgg ttccattgcc atttgaattt gttttattac
    10261 aaataacgac aggaatggtt gcaatctaca gcctaaaaga tttggctcaa cgctcccagc
    10321 tagtacaatc agctatcatt attgttgtta gctactgctt aatttatttt ggatatggca
    10381 ttacaattga tggagatatt actcgcatta attggactat gtttatttat ttctttgtaa
    10441 atggagtcct tttattattt gcttatcctc tcatttatat tttagaaaaa atgtttggat
    10501 atatttctaa cgtaacatta attgaattag ccaatacaaa taataaggta ctccgtaatt
    10561 tatcggaact agcccctggt actttccagc attcgatgca agtgtcgaat ttggcttcag
    10621 agattgcaat taaggtagga gctaatccat tacttgctcg tacaggagcc atgtatcacg
    10681 acattgggaa aatgaaaaat ccggctttct ttacagaaaa tcagaacaaa ggaaataatc
    10741 ctcatcaaca gttgagtttt gaagatagtg ctcgtattat tatccaacat gttgaagatg
    10801 gtgtgcaaat agccaaagat gcaaacatcc ccaattgtat aattgaattt atccaaacac
    10861 atcacggcac aaacaaagtt cgattttttt acaattcgta ccgtttgcaa ttccctgaga
    10921 aagaaattga cgatagtata tttacatacc ctggaccaaa cccaagtacg aaagaaacag
    10981 ctattgttat gttatgtgat gcagtagagg ctgcttcacg tagtttgcct gaatatacag
    11041 acgaagctat tgatggttta gtagaaaaaa ttatcacatc attattacaa gaaggctcgt
    11101 taaatgaagc acccttaaca atgcaacata tatcattgac aaaactcata ttaaaagaga
    11161 aactaaagaa catttaccac actagaatat cttatcctga attacaaaaa aataaattaa
    11221 aacccaatga aaccacttag actgtttact ataagtttat tattactgtt tactggctat
    11281 tttgtaaaat cacaaaatat tggtattcaa aaaaataacc ctgatttttt ggaatatctt
    11341 tcatcagaag agatacaaac gggttatgtt cctttaccct tcgaaatgcc cacaacacct
    11401 gttggaacaa agaggagcgg agaaagtttc ccttcacaat tcgactctcg caatgtgaat
    11461 ggaactagtt atattttagc tccaagaaat caggtaacaa ctggtccttg ttgggctttt
    11521 gcagctacag atgtggtaca agcttctttt tataaaaaag gatatcaaga aggatattta
    11581 tcaccacaac tattaacaaa ctgtcataac ttcgtgtata caaaaacaca aggaggaaat
    11641 tcgttgatgg cagtagctac atttgcccgt ttagttggtc ctgcttttga gaatgcggca
    11701 ccatatgatg cgaatgaaac agcctgtatg acaacaacaa aagaagactt tccagcattt
    11761 gtttcgaacg tacatatgat aaataataat attgatgcgg taaaaaaagc aatcatggac
    11821 aatggatcgg ttgcaacaaa aatgttttac gatgtcgctt attataatag tgctgataaa
    11881 acgtattttt ataatggagc agccaacgaa aatcatggta ttagcattgt gggttggaac
    11941 gataatatgg tggttgccga caaaggatat ggaactccat cagcttcggg tgtttggata
    12001 gtaaaaaaca cttggggtac tggttttgga gatgggggtt atttttatgt atcgtacgaa
    12061 gatacatatg ttgggaaaga ggtatttgca tttaccaagc gtattgataa aaataccatt
    12121 gacaaggtat atcaatatgc acagcatggg atgacaaata gtgtaggcta tactgccaat
    12181 aacccatcat acggacgcat taaatttact accactgaac gagagaaaat agcagccata
    12241 ggtttgtata cataccaccc caataccgag attacatttt ggatttacga aggaactgat
    12301 tttagcaaat tacgctatac atccgaagca gaagtaattc aatatggagg atattataca
    12361 atagatatta ctgcaccaat atatataact ggagattttc aggtaatagt aagctataaa
    12421 cactcaacgg aaaattatgc aatacccata gaacaaaatt tttctaacta caataatgct
    12481 acaatacaaa actcgggagt acagtggatc agctcttctg gcagttcatg ggctcctcta
    12541 ggtgcagata catcttataa atttaatctg tgtattaatg catatacctc tataccaaac
    12601 cctcctgcta acgataatgt gcaagatgct gtattgctaa acacaaatac agtgtacgaa
    12661 aacttcgcaa acgctggagc tacagtacaa gctaatgaag ttgtgccacc atacaataac
    12721 gaagaagcaa gttgttatac acaaaacagt tggtgttacg aaggggggtt acaaaattcg
    12781 gtatggttta aatttgtagc aactaactct gtagtaaata taattacaga aggctttgat
    12841 aatcagatag ccctttggca atcaaatgat gataatgcgg aaaaccttat cagtggcaac
    12901 acatctacct atcagctact agcagccaac gatgatgcca gtgtaagcaa tgcctttgct
    12961 tcattgtata atgtgcaagg attgcaagta gggaaaactt attttatcca agtagatggt
    13021 tctgccaatg gtgcagaggg tattttctca attgaaatac aagacattcc aacttctatt
    13081 aatagcaccc atgcatctac tttattaaca cctgaaggcg agttaacaca agaaataaaa
    13141 gataaggtga actatattga ggtgtttgat gtaagtggac gattactaca gatattgcca
    13201 aaaaatagta gtttgagcaa cagtaaatta cctaaacagc acgtgctaat catcaagttg
    13261 cattatactg atggtggtag cgataccatt agttggttgc aaaactaacg aattgactta
    13321 aaaaaatcct ttgatgcaag tcaaaggatt ttttgtttta gcattcgaca tcaatacatg
    13381 ccctttgaaa aactacttgt ttgataaaac cagccacttc tacgtgcttg ggatgcactt
    13441 gataggtttg caagtcctct acagaattaa atactgtatc taatactaca tcgtaattgt
    13501 ccgctgggca taaaggatga ttgatggcaa cagataattg cttgatttcg gtaatgtgtg
    13561 gaggcaaggt ttgtaataag tggcaaattt cggtgcataa ctccagcttg gttttgccta
    13621 aagcttcttc tttcaccttc cacataacaa tgtgtcgcaa cataagggta ttatttaaaa
    13681 attatttcgg cacaaatata cactattcag gcatatttag agaatatcaa gctatatttg
    13741 taaaaaaaat tcagcgtata tgagcattat tgaaagactt gaacaagaaa gaaatgcagc
    13801 taaagcacaa attttgcaag gattttttaa aactggcaaa ggacaatacg gcgaaggcga
    13861 tattttttgg ggcatttcgg tacctaccct acgtggcatt gccaaaacgt attaccaaca
    13921 aatacccaca gaagaaatta cccagttgct acactcgccc attcacgaac agcgtttact
    13981 ttcactgtat atcctgatat tgcggtacaa ccacgccaaa gatgataacg aaaaacaagc
    14041 catcattgat acctacttga gcaatacccg atacattaat aattgggact tggtagattc
    14101 gtcggcttac cacttgttgg gcaaacatta tgttgacaaa ccacgcacta ttctttttca
    14161 attagtgcac agtagcgatt tgtgggaaca acgcattgcc atcatcgcca ccttttattt
    14221 tatcagacaa cacgattatg ccgacacctt gcgtatggca ggtgtcttac tgcatcatcc
    14281 ccacgacttg atacacaagg ctgtgggctg gatgatacgt gaagttggca acagggatta
    14341 cggaacagca tacgcttttt tggtagaaca ttacgcacag atgcctcgca ccatgctacg
    14401 ctacgccatc gaaaaatttg acgagcctat acggcttgct tttttgaagg gggaagtata
    14461 actgagtgga atacttttta taagtagctt atttggtgcc cttgaaaaag aatatttctt
    14521 ttggtgttat cctagagatt acttttttta gactttttgt gtaaagttgt atttggacga
    14581 gttagtgaag cgtgggctgt atgtttcatt attccaatca ttacgcaact ttctgtttgt
    14641 aaagatgttc ctgaaattcg atgaacagtc ggcttatgtt tgctacgtct cctaaaattt
    14701 cttttgcatc ttctaatgat tgatacttgt ttgtcagtaa aattgggagt ttatcttggt
    14761 cgagttcgtc aacgcctgtt tcaatgtatt tgctcaatac gaatgaaata aactctttct
    14821 gcttgtcatt gaggaaggca aatattgttg cttcggctgc tttggctctg gcttctcttg
    14881 tcattgcgat atagtcgcca ttgaaaacat attccaacac atcgtacaaa tcacttttct
    14941 ccatatctac taacttttgt aaagtcagta agtcgtcttt gggaaaacca gctgcgtcta
    15001 aattttctaa taaaattctt cgggttatcg gactgctcca aagttttcgc aattcttctt
    15061 cgtctttgaa gagtttaggt aactctccaa acagattatt caaaaattct tctgctgaaa
    15121 tcggtttacc gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgcgaa
    15241 tgtttcttgc atcaactcca gttgaaagtt tttgcgaagt agtcaaaatg gttgggattg
    15301 ttttttcgtt gtcttgaaat tctcgcaaca atctttcacc ttcttctcca tcgttggcag
    15361 ttacacgaac acaataaaaa gggtctatac ttttggcgtt ttggtttacc aaatcccgaa
    15421 ttaatgcagc gtgagcttga tttgcacaaa atataattgc cttttcattt tgattggctt
    15481 cttctaaaaa gatatttacc cgcttggctt cacgtgccac aatttcaatc gtgcggttaa
    15541 agtctttttc ttcatacaat ttgccttctt caatttctcc ttctacaatc gtgtcgtctg
    15601 atgtgtagcg gtaatcatcc aaagttgttt tgatgcgttt tactttgaaa ggcgttaaga
    15661 aaccgtcatt gataccttct tttagagaat atatgtaaac aggttcgcca aagtaacggt
    15721 aagtgtccac attgtctttt cgtttgggag tagcggttaa acccaactgt actgccggag
    15781 taaaatattc taaaatgctt ctccaattgc tttcgtcatt ggctccgcca cggtggcact
    15841 cgtcaataat gatgaaatcg aaataatcgg ctggatattc gccaaagtag ggtgcaggtt
    15901 ttccttctgc atccgtgccg ctcataaagg tttgaaaaat ggtaaagaaa atactgccat
    15961 tggttggcac ttttccgttt ttcttaatct cggttggttt tatccgtacc aaagcatctt
    16021 cgggaaatgc ggaaaatgaa ttgtaagctt ggtcggctaa aatatttcta tcggctaaaa
    16081 acagaattct tggtctacgg ttgccgtctc gttgtaagtt ccaacgggtt tgaaacagtt
    16141 tccaagctat ttgaaaagca attgccgttt ttcctgttcc tgtggcgagt gtaagtaaaa
    16201 ttctttcgct tttgttggca atggcttcaa cggttcgttg aacggctatt tcctgatagt
    16261 atcttaactg ccaacttccg cttttatctt caaatggaac attggcaaat ttttctctcc
    16321 aatcgttttg ctcggcaaag gttttgttcc aaagttcgtc tgggctcaga aaatcgttta
    16381 ccaaaccttc ttcgcctgat ttcatacaga tttggtaaat ggcttttccg ttggtgctgt
    16441 aagtggtttc taattttagt ttgtctgcat atttcttggc ttgcattacg ccttcgccca
    16501 cttccaattc atcacttttt gcttctacta tagcgagttt gataccttta tgtaccaata
    16561 cataatcggc aatttctttt ttgccacgcc caccgcctgt ttgtattttt ccttgcgtaa
    16621 tgtggtattc acgaaggact ttagagcctt ccacaacgcc ccaaccacaa gccttcaact
    16681 tggggtcgat taattccgct cttgtttctg cttcgttcat tttcaactaa ttattctcgc
    16741 tactaatagc ttctgtatat ttctctattg cacttttata taattgttcg ctttcatctt
    16801 tgaaacgact tttattttcg tttaattcat tgaatttttg ataaagatgt ttgtaaatga
    16861 tttgatgtgc tttattttgt aaagcagctg tatcaaactc atccatttca aaatcgccag
    16921 aaatagcatt atctaataaa gtcagtaaat cttcctttcc tatttggtca atccctatcc
    16981 attggtcagg ttctttaaaa taaagccctt ttccgttatc aatttttaag attttcatat
    17041 gattgctttc tttcaacata tgctgaatca cctgcttcaa ggcaattaag cattatatta
    17101 taattattaa tttcttcacc atcaacggtt ttaagaattg tatcagctgg attacctgaa
    17161 aatattttgt aaactgcttt accgtcaact atttcctttt tcgtgtaaag aatgtaatca
    17221 ggttttattg aggctccaac ggtgttatta tgagtaacga caataactgg tacagtcttg
    17281 gaaatctgtc ttatttgctc atttacttcg tttttcaaaa acaaattatc aaatgaagat
    17341 tcaggctcat caattaaaag aaaatcgtaa tggtgtgcat gttgtattct atcaagtaaa
    17401 ttgaattcag aacgttcacc gcctgaaact tctgcatcat gttcattcaa tattttataa
    17461 cttacatcca caaaatattt ataaaattca gtttctggta ttcgttccat tttctttagt
    17521 tcttccaaaa attgaatagg ggaatcatag agtggaaatg caccactaaa agcagtttgt
    17581 tgtatgccgc ttttattttt taactcttgt gcacctgtaa atctttttgt tgatgctact
    17641 attttaaacc tacgaatatc cttttgctca attacttttt ccttttttat ttgggctgca
    17701 atgttttcaa acttttctag cttttctttc tcaagtaaaa cattgtaaaa atcaatatct
    17761 ttaacgtgct cggaagcagt tgaagattgt aattcctttt gaacatttgt gacaagtgaa
    17821 ttaacccatc ttacttttaa gttcttctgt tgaacttctt catatttctt cattaaatca
    17881 acagccaatt cttgcaaaat cttcttagaa atatgcttat ctattagggt tctatactgt
    17941 gcattttcaa taagtaattc cgttgcttct actaattgct ttaatgtttc gttattggtt
    18001 tcgataaatt tactctcatt aaacagtgtg cattttgaat atacatccat tttatcttct
    18061 tccgctgcaa cttttaataa acttgttaag tagctttcta ttcgtttgtc attttctttt
    18121 ctgtcaattt ttaatacatc ttcaacaact tccttaaact cttttaagaa ttgttcggaa
    18181 acactgcttt gcttggttgt aagcaattca tcaaatttct ttttgtcttt ttcttcatct
    18241 gtttctaaaa gttcgaattg cttaatgtgt ttaacatttt catacaatcg agaaattgtt
    18301 ttgagtgtat aagttttacc tgatgacctt tctcccaata tcacatttaa accagttgag
    18361 agcttttgtc cattctcgaa tatttgaaaa aaacgatgac cttcactatg gtgcagaaaa
    18421 actttatttt tatccataag gcaggtttta attgccctga gagacttatc gtctatatca
    18481 ataaaagtct gtcgaggtga aaattcagtc atttgatgag taaaacgcaa atcactgaat
    18541 aatactggaa ctaaactatt tgtgtcgtta atgcaatatt tgaatttttt tgcacttgaa
    18601 acttctcccg aggttatgaa tggttttaat tgttcaatta tttcaggttt taaaatcggg
    18661 tctttatcat agtgcggtac caacaaatat ttacttaaat cgacaaaaat ctccttcaat
    18721 tgagcaatgg agatataatc tgacttgttt tgaatcaaat gctcaacttg attacattta
    18781 gttttaaaat catcaagttc ggtactttca gaaattaaaa gtaaatgccc tttttcaata
    18841 tctatttcaa ttcctggtag aactgtaatt tcaatctgtt cggaaatttg attaaattgt
    18901 tcaaggtcaa ataaattgtg attagttata gcaatacaat caatagacaa tttttcaacg
    18961 tattcctgta atttggaaat gctgaaatca taaacagaat cactatacgg tgatgctttt
    19021 gtatgaatat gtaagtctat ttttttcata cttgttctgc aaatacttta atttcagttt
    19081 tcaactcgcc cccaaatgcc ttttgcaaga ttgatttttt cagttcttcc aaatcagcga
    19141 ttttcttttg atacaccgct tccaactttt gcgtttcggc tcgtagtgca tctaattggt
    19201 ggacaatggt ttgttgttct tcaattgatt ttggaaaaga aatttgatac ttatttagtt
    19261 tttctattgt caaggcacta tatgcagcac caattgcaag gctttttatt ttttctacaa
    19321 atgcagtcaa agcaaatttt aaataattcg tgtctaaatt tctaaatcct ttaaaccaaa
    19381 caatgtttcc atccttaaaa tagaattcat ctttattttt aataaccatt atttcgccaa
    19441 ttgttcctac tgctgacatc agtaaatcat taatctgagg aacgccaaat gattttttta
    19501 tttcattgta tctttctctt gaaatgaata attctaacgt tatatttttt ccatttgcta
    19561 attccttaat ttcctttgtt ctataaaaag gaataccatc ttttacatat tcgcttttgt
    19621 aaattcgttt acttgaagta attgttccta attcatctaa acatttggtt tcccagtttt
    19681 cattcccatt ctcaaacacc tcctgcaaat agctttcaaa aagttctttg gcgtttttga
    19741 ggttttgttc ggcattggct tttgccttgg ctatggcggc aaaggcttca tctaatatgg
    19801 aaacaatgcg ttgttgttcg gggagagggg gtattggaac ttctactgaa ctaaccaacg
    19861 caatattcaa atttcttact gttgaacctg ctgccaaata atcgaattga ttaaaaacat
    19921 acggtgatga aagtaagtag tataaaaact ctgtatcgaa aatcttttga ccattctctt
    19981 ttaatactaa ccaaccatcg tgaatgcaac ctgaagtttt cattatgtaa ggtctgccaa
    20041 aactcattga atttgataaa atgaaatcgc cttcatttac cattcgtgtt ttgtgcaaac
    20101 cttctttggt aattttttcc tttgtctcaa aaatgtattt atcagaagct gttgcatctg
    20161 aaatttttat ccaattaatt ccttctggtg aattggttaa atacttgtca atcggacgtg
    20221 gagaaccacc ccgctcaatt gttaagactt cacccaattt cttttttatc caattcatat
    20281 caattctgaa attgagttta gtatttcggc actttctttg tccaatgctt tcatttcttc
    20341 caaaatggct tgcggttggc gtagggctgt ttcttctttt ttgttcgggt tttttacgct
    20401 caaatcaaaa gtgttttggt caatgtcttt ggtgtttacc gtccaagagt tttcgctatc
    20461 ggcaaaggtt ttttgtagtt ccacaaattc tgccaaatcg ttttcgttga gcggattggt
    20521 tttacccaaa ttacggtcaa gatttagcga gtaaaaccaa gtcttttggg tgggtttacc
    20581 tttttcaaaa aacaacacaa cggtttttac acctgcaccg gtaaacgtac cgcccggtaa
    20641 atccaacacg gtatgcaggt tgcagttttc caacaaggtt ttgcgaatgg caacggtggc
    20701 attgtcggta ttgctcaaaa aagtgttttt aataacaaca cctgcttttc cacctgcttt
    20761 cagaatttta ataaagtgct gcaaaaacaa actggcggtt tcgccggttt ttattgggaa
    20821 attttgctgc acttctgctc tttctttacc accaaaaggc ggattggcaa gcactacatc
    20881 gtaacggtct ttttcttgta tgtcggcaag attttcggca agtgtgttgg tgtgtatgat
    20941 gttgggagct tccacgccgt gcaaaatcat attcataatg ccaataatgt aagccagcga
    21001 tttcttctct tttccgtaaa aggttcgttt ttgcagtatc tcggtttctt tggtagaaag
    21061 tgatttgcta tttctcagat aatcgaacgc ttcgcaaagg aagcccgcag aaccaacagc
    21121 accgtcataa attttgtcgc caatttttgg ggcaaccact tttacaatgg tggtaatgag
    21181 cggtcggggc gtatagtatt cgcctccgtt tcgccctgcg ttgcccatat ttttgatttt
    21241 gtcttcatac agatggctca tttcgtgttt ctctgcgtgg gtgcggaaac gcaactcgtc
    21301 aattcggtta atcacttcac gtaaattgta gccgctatgt atgcggtttt tcagttcgct
    21361 aaaaatctcg ccaatcttgt attcaatcgt gtcggcactc tcggcactta gtttaaactt
    21421 tttgaggtag ggaaacagtt tgttgtttac aaagtcggtg aggtcgtcac ctgtcagggc
    21481 tttgtggtgg tcgagttttc cgtctttgcc ttttggcatt gcccaaactg tccattggta
    21541 atctttgtca atgataggcg tgtaagtctt gcctgtaagt tcggctgctg tggctcggtc
    21601 tttctctaag tcgtccaaat acttgaggaa caaaatccac gaggtttgct ccacatagtc
    21661 caattcactg ccgcaacctg cgtctttgtg aagtatatcg tcaatatttt taaaagtctg
    21721 ctcgaacatt attaaaaccc tgtattataa tcattatcaa taaacgcaaa tgtacaaatt
    21781 tctgtggaat tgttggcaag taaactgctc tttttttcca gatgaaaaat cgggatgttt
    21841 cggagcttgt tctaaaagct ttcggaatac tgctttgtgt gttggcggtt cattattttg
    21901 tttcccttta ttctgtcttt aagtagcaaa ttgttagctg tatagttatc ttttcactga
    21961 ctgccaacat caatgtaagt gtactacccc tacacctata caatataata ctaacaatac
    22021 tctattcttt ttggcaaagt taaaaaaaga aaagaaagaa ccgagtattt tgagaagaat
    22081 aattgatgta aacaattttt gcaagtttgg gaacttgcga aaaagaaaaa acgttaacag
    22141 ctaccgaaat agtttgttaa cgttttcctc tctgttgtct caccaggatt cgaacctaga
    22201 cagacagaac caaaaactgt agtgctacca ttacaccatg agacaatcaa atctattgca
    22261 aaagttacct tatcgcaaat gtggtgcaaa ggtagtatgt aagatttaat tcaccaaata
    22321 tttttctaaa aaaatcactt cacacggagc aaatagtagt tctttttgcc tttttgtacc
    22381 aatataaagg tattgttgag taataaattg ctagtagcga tggcttcggt atcggtaatt
    22441 ttttctttat tgataaacaa gcctccgttt tgtatcattt tacgcatttc gcctttcgag
    22501 gcaaatacag gagcttgtgt ggtaagtaaa tccatcagtt ttacttctcc cgacaaatcc
    22561 tttgtatcaa tttcgaattg aggcacacct tcgaatattg ccaataaggt ggcggtatca
    22621 atctttttaa gcgtttcgga agtggcgtta ccaaacaata cagccgaggc ttctactgcc
    22681 atttcgtacg cttcttgcga atgtaccatg caggtaactt cttgtgccaa gcgtttttgc
    22741 aatagtcgca aatgaggtgc ttcgaggtgc gttgctacta aactttcaat ttcttctttg
    22801 gaaataaacg taaatatttt gatgtatttt tgagcatctt catcgcttac attcagccaa
    22861 aactgataaa aatgatatgg tgaggtatat tttgcatcta accaaacatt gccactttct
    22921 gttttaccaa atttgccacc atcggctttg gtaataagcg gacaagtaag tgcaaaagcc
    22981 tctccaccta cttttctgcg aattaactcc gtaccagtgg taatattgcc ccattggtct
    23041 gagccaccca tttgtagttt gcagttttta gcatcgtata aatataaaaa gtcgtagcct
    23101 tgtaccaatt gataagtaaa ttccgtaaac gacatgccta cagaagattc ggaagacagg
    23161 cgttttttta cagaatcttt tgccatcata taattcactg tgatgtgttt gcccacgtct
    23221 ctgataaaat ctaaaaacgt aaaggatttc atccaatcgt aattgtttac aatttcggct
    23281 gcattgggag catcgctatc aaaatcgaga aaacgggcta attgttgctt aattgcatct
    23341 tggttgtgac gtaaggtctt ttcatcaagc aagttacgct cagcgctttt gcccgatggg
    23401 tcgccaatca taccgcttgc acctcctatt aaggcaatgg gtttgtgtcc gcaacgttga
    23461 aaatgcttga gcatcattac acccaccaaa tgacctatat gcaaggaatc tgccgttggg
    23521 tcaataccta cataagcaga ggttaattct ttttctaatt gttcttttgt acctggcatt
    23581 acggtatgta tcatacctct ccattctaat tcatcaacaa aattcatgtt attatcgttt
    23641 agttataaaa aaatctttca ttatttgagc acattcatct gccatcacgc cagccacaac
    23701 ttccgtttta ggatgcaggg ctaaaggaac ttttttggta tatccatatt tatcttcgct
    23761 tgctccatat actactttgg ctacttttga ccagtacaat gcaccagcac acattacaca
    23821 gggttctact gttacataca aagtgcaatc attcaggtat ttagctccta aataacttgc
    23881 agcagcagta attgcttgca tttcggcatg ggcggtaaca tcatgcaatg tttcggtaaa
    23941 attatgagca cgggcaataa tctgttggtt acataccacc accgcaccaa taggaacttc
    24001 tcctttagca aacgcttttt tagcctctgc tagggcttgt ttcataaaat acgtatcgtc
    24061 taacattatt cttgcaaatc ttcgggtagt tgtttacttg tttttgcccc tagctgtttc
    24121 attttttcgg cagtaacaaa tacgtttcct gctcctaatt gcagtttttt catggcatcg
    24181 ttatgcgaag ctgaggctct atcaatattt aacttcacct tgtttaaatc ttctacacaa
    24241 cccactaatt tatcgtacaa agctccaccc aaacgtgcaa tttcttgggc atttttggtt
    24301 tgattttctt gtttccagat ggaggctatg gtacgcaaag tagccaataa ggtagtagga
    24361 cttaccacca caatctttct atcccaagca aagctataaa tctcattatc agactgtaat
    24421 gctaccgaaa aagagccctc gataggcaaa aacattaata caaaatcagg agaatttaat
    24481 gttggagaat tttgataaca cttttcgcca agtcctttta tatgattgcg aagagaaact
    24541 atatgagcct tgaggtacgc atccttctca tcgtcgtttg cattcacaaa attttcatac
    24601 gctagtaacg acacctttga atccacaata atatgcttat tatcaggcaa atggacgata
    24661 acgtcaggtc tatatacttg acctacatcc ccttcaattt gttcttcgcg tgtatattca
    24721 atcccttttc gcaagcccga acgctccaat accctttcga gtacaatttc gccccagtta
    24781 ccttgctttt ttacatcacc ttttaaagct ttcgtcagat tagtggcttc ttcgctgata
    24841 cgcgtattta gctcagttaa ttttcgtact tcttcacgca aacttagctt gtctctaagc
    24901 tccttatcgt aagcatcttc cactttcttt tcgaacaaag aaattttatc ttttaatgga
    24961 ttgacaatat cggtaaggtg ttttacattt acatctgtaa aatcagaaga attttgtttc
    25021 aaaatgcgat tggcaatgtt ctcgaactct ttggtaaaat gttgttgcaa ttcggtaagt
    25081 tctttttttt gcgtgtctaa tcgttcggac aagtgcgtat tttgtgtttg caacacagca
    25141 tactgagagg atagttgttg gtatttttca gtttctttac taagttgttg taaggtttgc
    25201 tgatacacat tttgcacatc agccagttgc atttgagcca aggctttctc cttttctaat
    25261 accgcaatag aactggcaag agctttttct tgctcggcta atttattgtg cataagctgt
    25321 ttcatgtact ttacaataag ccatagcaca gcaagcacac ttactaaccc aatacttatc
    25381 cacatccatt ccattgtata tcttttttaa tcaaacaaag ttggactact ttgttctagt
    25441 cgttcaataa ccgttgacat taaaacagtc cttacaaaac gtgatttatt tttcacccta
    25501 aaacgtttac aatatgcctc caacgcattt aattctttgt cgtttaacac aaaagtaact
    25561 ctattttttc tgagtaatga tttttgtgta tttttgtcgt tacgtttgct aggttttttc
    25621 attcactaga ttttttttct atctaccttg caaaagtaca acggtttata taattaaaaa
    25681 aatgctttta agaaaaaacg taataaattt gtaaaataaa tggcgataca caacaaactg
    25741 ggagaagaag gagagaaatt agctactgca tatttgcaac aaaaagggta tcgtatccgc
    25801 cataccaatt ggcgtagtgg acatttagaa ttggatattg tggctgaatc gcacgatacg
    25861 ctttatgttg ttgaagtaaa aacacgcagt actgcctact ttgaacatcc caaagatgcg
    25921 attaccaatg ccaaaataaa acgcattgtg catgcaacag aagaatacat tcatcggttt
    25981 gatatccaca aagatataca gtttgacatt atggctctac tgcctaataa gcatggaaca
    26041 tttgatattg aacatattcc agatgcattt ttagccccac tttcataaaa aaagcttaga
    26101 caatgaacat tgaagaagta cgaacatatt gtttgtcaaa gccttatgtt actgaggatt
    26161 ttccttttga cgagaccgta ttggtattta gagttgctgg aaaaatattt gtattgtgtg
    26221 ctcttgatgc catccctcct cgcatcaatg taaaatgcaa ccctcaaaaa gccattgagc
    26281 tgagagaatt gtacaactat gtagtaccag ggtttcacat gagcaaaaaa cattggaata
    26341 cgatattcat agaatctgtt gacaatgagc aacaactccg cacttggata gacgaatcgt
    26401 acgaattagt gattaaagga ttgacaaaaa aggagcaaaa acaactgaat atgtaattat
    26461 tttaggaatg gactccaatt tttatacatt atttgtatat ttgcaagtta tagttttttt
    26521 gcataaaaca accaataaaa acctaaactt ataattatga aattagcctt gattggatat
    26581 ggtaaaatgg gaaaagaaat tgaacaaatt gccatttctc gtggacacga aatagtaagc
    26641 agaatagatg ttgacaacca agaagatttt aagtcagacg cgtttaaaag tgccgatgta
    26701 gcgatagaat ttacggcgcc tcatgtggct tatgccaatt acttaaaggc atttgaagca
    26761 ggcgtttcga tagtttcggg cactactgga tggcttgata aaattaatga aattaaagca
    26821 atttgccacg acaagcaact ggcatttttt tgggcatcca atttcagttt aggagttaat
    26881 atttttgctg cagtaaatag ctatttggca aaaattatga atcaatattc tgaatacaat
    26941 gtttctatga cagaagtaca tcatacacaa aaattagatg caccgagtgg aactgccatt
    27001 acattggctg aaggaatcat taaggagcta gcacgtataa ataaatggga attagaaacg
    27061 gcatctacaa aagattcgct ggcaatacat gctattagag agggaaatgt acctggcata
    27121 cacacaatta ggtacgaatc ggaggtagat agcattgaaa taacgcacga tgctaaaagc
    27181 cgcaaagggt ttgcattggg tgcggttttg gctgctgagt ttacgtatgg caaacaagga
    27241 ttcttgggca tgaatgattt attacaattt taaacaaaga taaaaaagat gagcgatata
    27301 cataacgaaa aaaaaactgg gcgttttgat gtttcactaa tacaatggat aaaagcgttt
    27361 gttgtatgct tgctatacgt actatttatt atctgggtag gaaatttttg gtggttatta
    27421 ctacttccta tattttttga tatctatatc acgaagttta ttccttggag tttttggaaa
    27481 aaatcgaaaa ataaatatgt ccttaaaatt gctgaatgga cagatgctat cgtatttgca
    27541 ttgattgcgg tgtattttat caacttattt ttattccaaa attataaaat tcctacctca
    27601 tcattggaaa aatcattgtt ggtaggcgat tttttgttag taagcaaagt aagctttgga
    27661 ccccgagttc caaacacgcc actttctttt ccattggcac aacacacatt acctatactc
    27721 aataccaaat cgtatattga ataccctcag tgggaatata aacgattgaa agggctagat
    27781 acaattaaac atttagatat tgtagtattt aattttcctg cgggcgacac agtaacaaca
    27841 aaagtatcaa atccagatta ctatacatta tgtcatgatt acggaagaga atatgtacac
    27901 aataataaaa atttgtttgg agacgtcatc tatcgcccag tagataggcg agaaaactat
    27961 gtaaaaagat gcgtaggatt acctggcgac acattacaaa taattgaaca ccaagtatat
    28021 ataaatggag ttgcccaaga agaagtagaa ggcacccaat taagttattg ggttacgact
    28081 gacggtagaa aaatttcgga aaaccaattt aaacgtatag gggtaagcaa agatgaccaa
    28141 caattcttaa tcaataacct gccaaatggt gataacttgc tggattattg tggctatgaa
    28201 agaaatagtg aaggcaaaca caatccagta tacagattac cattaaccaa agaggcttta
    28261 aacaaggtaa aaaatttacc gaatgtaatc aaaatagaaa ttgataatgg attaatttca
    28321 ggtcaagtat tcccatttgg ttataaaaaa tggacccgcg ataattatgg acctatttat
    28381 attcctcgca aaggggctac cattgcatta aatttggata atttacccgt atatgaacgt
    28441 gtaattcgca attacgaagg caacacttta gaaattaagg atagtacaat ttatataaat
    28501 gctgttgcaa cgactaatta cacctttaag atgaattatt attggttaat gggagataac
    28561 cgccacaatt cagcagattc tcgttattgg ggatttgtgc cagaagatca tgtggtggga
    28621 aaaccactat ttgtatggtt atcattagac aaagatgcat cgttactaga aagcattaga
    28681 tgggaaagat tatttaaatt tgtgcataaa gaataagaaa tgagcggtgt agtactcaca
    28741 tccacgtatt tagggaatat tcagttttac agtaaattgg tacacttttc tgaaatttgg
    28801 atagaacaac attgccacta tacaaaacaa acctatcgaa ataggtgtaa aattgctact
    28861 gcaaacggag ttatggcgtt atcaatacca accataaagc ctgcaacaga aaaatgcaag
    28921 acaaaagata tccgcataga taatagccag cattggcaac aaatacactg gagggcttta
    28981 gaagcagggt ataattcgag tccatttttt gagtattaca aagatgattt tgctcctttt
    29041 tatactaaca agtttgaatt cttagtagac tacacctttc aattacaatc gttggttatt
    29101 gcattattgg aactcgactg taaggtgcaa ttgacagatg catataccac atacgatgat
    29161 gcgttagatt acagagatgc actatctgca aaaaaagaaa tagaagataa cgattttgta
    29221 attagttcgt attatcaagt atttgaaaat aaatttggtt ttattcctaa tttaagtatt
    29281 cttgatttgt tgtttaatat gggaaatgaa tccattttag tattgcaaaa atctgctagc
    29341 aagcattaaa attttgacaa tgaaacagtt tttagtcgta atattggcgt ttttttctat
    29401 tggcgttggc acaagctacg ctcagataca aagtg
//
LOCUS       DAOSRP010000005        28900 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1784_length_28900_cov_4.756041, whole genome shotgun sequence.
ACCESSION   DAOSRP010000005 DAOSRP010000000
VERSION     DAOSRP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28900
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1784_length_28900_cov_4.756041"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLJ82_00710"
     CDS             complement(<1..336)
                     /locus_tag="PLJ82_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR40064.1"
                     /translation="MSIYKTAVNKPITSILIFVGVIIMGLYSLIQLPIDQFPEIEIPS
                     VTVMTSYPGANGTEVETNVSKILENSLNSVDGLKEVYSTSKDNLSLVTLEFEWGANLD
                     EALNDVRSSI"
     gene            complement(351..1373)
                     /locus_tag="PLJ82_00715"
     CDS             complement(351..1373)
                     /locus_tag="PLJ82_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR40065.1"
                     /translation="MKKGIKISMLMLAVIALLNACTPKAEKKQEEEIRVENVKTAVLE
                     TQEIARKIDFSTTLEPYNKVMVAPTMAGRIDKIHVEVGSKVQANQLLVEMDKTTYLQT
                     KVSFDNLAVDFQRISTLYNSGNIAQQTYDQTKAQYESLKTNVQNLERNTFLRAPFSGV
                     IAAKNYENGELYAGTPILLLVDMANLKAHINIPESYYPSIKEGMNVAITSDVFADKEF
                     KGIIEIVSPVIDATTHTFQVQIKISNAGGVLRPGMFARIGVDFDKINAKVVPYQAVLK
                     LQGSNERYVYVNKNGVAKRVKVQMGKRYDDKIEILSDELKEGDQLVVVGQARLVNGVQ
                     LTIVND"
     gene            complement(1401..2690)
                     /locus_tag="PLJ82_00720"
     CDS             complement(1401..2690)
                     /locus_tag="PLJ82_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR40066.1"
                     /translation="MKRKLFLLFASFFLGYSVHSIAQESLHISIDEAKEHALNYNKTI
                     QKANLAIQKADYAKWQSIASLLPKVDASLGYNNYLGHKMSLMGTPIAMNPEGSFTAQA
                     SIALSGMQVVGVQLSEIAQQMSQTSALSDVLSLQTNVTNAYYAILVSEESKKIVEGNL
                     ENLRKLYNSSLNLVKVGMAEQTDADQLAVQVGMIENTIRMAERNVELSYNTLRLLLGV
                     DNNTPLSLKNTLKDFTNKESANELLATSFDMTQNYTVTLLQQTIEMNQKQVRLNQWAY
                     APMLTGFYQYTKKTHFDKAVGFDMTPPNLVGVTISIPVFSSGERYAKVKQAQFDLQTA
                     KLNKEQTVDALLMEEKQLRYNLTSAIESYELQLKNVALYERILTNATQKYEQGMISST
                     ELSTISNNTLSTQSQYISALMDMLMAKTKLQQLINKL"
     gene            complement(2710..3318)
                     /locus_tag="PLJ82_00725"
     CDS             complement(2710..3318)
                     /locus_tag="PLJ82_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR40067.1"
                     /translation="MDKIKQEILKTATNAFVKKGIRSVSVDDICDNLRISKKTFYSYY
                     RQKEALVMAVMETFTTHHQEYHCMLQNQEFTNVIDEILWMKTLIKNGKEEKKHERFFN
                     DLLKYYPSIYNTFSVEKRQYTEQMFLIQIEKGIAQGVFREDIHVQFTAQSLTVSLQYF
                     INMSIKHPRKYTKRGIIDFMVDSYLRMVCNEKGLLYYTSLLT"
     gene            complement(3613..5196)
                     /locus_tag="PLJ82_00730"
     CDS             complement(3613..5196)
                     /locus_tag="PLJ82_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOR40068.1"
                     /translation="MKLKYLVCFVCFPIVLLAQQADYQKPRLVIGITVEGLTADFLSM
                     FWNGMGNQGFRKIVENGATLSSLQFDYDATDNVSDIATFSTGSQPYLHGVSWSDVYNR
                     NTRKYQFLLQDDAVKSINGVDQVSARGLLVTTLADELKIHSIQRSKVVSIAMNAKEGI
                     LQTGHLADVVMWIDDTTGKWSSSTYYNEQLPSWVNRKNEQNAIQNYMEMVWEPLYPIN
                     YYYVTQSRGFTTKSFSYPLKKMTMGKNGVKEFKKTPFANTCIREMAVEAIEQETLGRD
                     IFPDLLLLNFRLQSYPQASDLTVELEDAYVRLDNDIKMILEAVERKIGKEHILVYITS
                     PRKTHYAVSPLLKEKMQLGTFNSYRYMALLNVYLMAIYGQESWVMGFYNGNIYLNRKI
                     IDEKNLSLKEVQERAMEFVSQLPGVMNVVNSVALEQQFALRNSLRQSYNKQSSGDILF
                     TLYPGWVEVDKNDNPTGFYNVLGNSVPFYFYGWKIQPQTIATPYAIIDATATLAKWLQ
                     IPLPNASNGVLLPIVLSAE"
     gene            complement(5246..7030)
                     /locus_tag="PLJ82_00735"
     CDS             complement(5246..7030)
                     /locus_tag="PLJ82_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOR40069.1"
                     /translation="MKQTDIVNLWYLSLLKWRERKIKTSNFILVLSFLVGIFTALAAF
                     FLKSAIHFIQHILTTNFSVNHENYLYLAYPIIGIFITTLFVRYVVKDDISHGVTRILY
                     AISKRKSRLKPHNNWTSLVASSITIGFGGSVGAEAPIVLTGSSIGSNLGKFFKMDQKT
                     LMLLVGCGAAGAIGGIFKCPIAGVVFTLEVLMLDMTMTSVVPLLISSVTATSISYFLM
                     GNTVMFAADSYEPFLLERIPYYILLGIVCGLVSLYFTRAMNKMEGFFQRLKSMYAKLF
                     VGGVVLSLLIFVFPPLYGEGYDSISALLNNQPNILFEGSLFYTLKDNMIWVVMYMGFI
                     VLLKVFAASATNGSGGVGGIFAPSLFIGCITGFAFALVAGFIGEVLPVQNFALAGMAG
                     IISGVMHAPLTGIFLIAELSGGYELFIPIMIVSTVSFLTIIGFEPHSIYAMRLAKKGE
                     LRTHHKDKAVLNMLNMNRLVETDLYKVEPSATLGDLVKVIATSKRNLFPVVDKDGVFL
                     GVVLLEEIRNIMFRTELYQRFTVADLMIAPPSRIFVGEEMEEVMMKFEKTQAWNLPVL
                     DGDKYVGFVSKSTIFSAYRNVLVDFSEE"
     gene            complement(7046..8461)
                     /locus_tag="PLJ82_00740"
     CDS             complement(7046..8461)
                     /locus_tag="PLJ82_00740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOR40070.1"
                     /translation="MNHKKQVIKYILADYITAVLAWFLFYVFRRIEIDLVAIRDIQMF
                     VPMYNARVTMLLVPFFWIVLYVVSGYYNTPFFKSRLSEFFTTILSVFFGSLIVFFSVL
                     MDDPVASYTFYYISFFVYFILHFSLTYAFRLLITTQSAHLIHTRKIGLRTLVLGVGEK
                     AFKLEKQLSEMNQSLGYIIEGFVALQKNETRKVNNSSVVGSIVDINNVLKKYPVDVVI
                     IAVDSITEDDIYQLMQSLWSKNIKIKIVPSLFEILTGSVKMNSIYAVPLIDLLEIQMS
                     DFQLNLKRLFDILLSSMVLLIGLPFFVIIALRIKLDSKGEVLYKQERVGLHGIPFMMY
                     KFRTMYENAEQDGVPRLSCENDERITPFGRFMRKYRIDEFPQFYNVLKGEMAIVGPRP
                     ERRYFVDQIIEHAPYYYRLFTVRPGITSWGMVKYGYANTIEKMIERAQFDISYIENMS
                     LVIDSKILIYTIKTIVTGRGI"
     gene            complement(8481..9026)
                     /locus_tag="PLJ82_00745"
     CDS             complement(8481..9026)
                     /locus_tag="PLJ82_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOR40071.1"
                     /translation="MIKQNNIVVKQYVDELRNAHIAFCYFDNKYGVLCDATNENLVHK
                     MYTKLLPQRMYLLVDNVVKLRHYVSEIPEIAYDLWETSENNVLIVFSELQNVSATIQQ
                     NSANVALGVLSDGFLQNLLQQFRIPLLFIPFVSETDKEIAEIQHIIPYFNMVFDKNRK
                     LYAGYGIIKLQKDGTFVILRK"
     gene            9219..10943
                     /gene="recJ"
                     /locus_tag="PLJ82_00750"
     CDS             9219..10943
                     /gene="recJ"
                     /locus_tag="PLJ82_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOR40072.1"
                     /translation="MTNNWIVKELTTEESEQTEHLIKDLGISPILCKLLVQRGIHTME
                     EAKNFFRPQLSQLHDPFLLNDMDKAVARLNKAMRKKEKILIYGDYDVDGTTAVAVVYK
                     FLQQFYSSIDSYIPDRYEEGYGISYKGIDYAAENGFSLLIVLDCGIKAIEKVRYAKEK
                     GIDIIICDHHTPDEILPDAIAVLDPKRVDSTYPYPHLSGCGVGFKLIQAFAINNQIDF
                     DNITPFLDLLAVSIASDIVPITGENRILMFFGLKQLNANPCVGLQAIIDVCGLRGKEI
                     TTSDIVFKIGPRINASGRMRSGNEAVELLVSKNLSFAKAKSDCIDQYNQERKDLDKKT
                     TDDAKDFIQGNEDFEHRNSIVVYHPEWSKGVIGIVASRLSELYHKPTIVLTLSNGFAT
                     GSARSVTGFDIYKAIESCRDLLENFGGHMYAAGLTMKEDNVSIFTERFDNYVRENITE
                     EQTQPQIDIDAIIRFSDITPKFFNILQQFAPYGPENMKPIFATHKVTDFGSSRLVGKE
                     LEHLKLELTEPNSENVMNGIAFGMGEKFSAHIKDLKPIDICYTLEENVFNGNKTIQLM
                     IKDMKINE"
     gene            10964..12868
                     /locus_tag="PLJ82_00755"
     CDS             10964..12868
                     /locus_tag="PLJ82_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOR40073.1"
                     /translation="MYHEILHTYWGYSEFRSLQLDIIQSIGNGKDTLGLMPTGGGKSL
                     TFQVPALAKEGLCLVITPLIALMKDQVENLKKRGIKATAIYSGMSYEEMMIAIDNCMY
                     GNYKFLYVSPERLSTRAFLLKIPHLQINLIAVDESHCISQWGYDFRPSYRQIASIREH
                     LPTVPILALTATATPEVVRDIQTQLKFAAPNVFQKSFVRSNLIYIVRQTDDKLQQLLQ
                     ILQKVPGTSVVYVRNRKKTKEIADFLNENNIPANYFHAGLSNNTKDERQQKWKNDECR
                     VMVSTNAFGMGIDKPEVRTVIHLDLPDSIENYFQEAGRAGRDEKKAYAILLYSNADKV
                     KIEKRIRDNFPEKEKIKEIYEAISNYLQIPVEGKEGAVYDFEIFDFCAKFHFPALLVH
                     SAINILEQAEYICYTEEVDSNSRILFTVSRDDLYAINNSHIHSEKIIHALLRSYTGLF
                     TDYAYINEEIIAQRAGLTVDETIQTLIFLAKTKVIQYIPRKKTPFITYTQQRQEKRYL
                     KIPREVYEEKKERYVKRIESMIAYATNTTVCRSRMLLHYFGESNTHDCDQCDICLTRK
                     KNDLANQEFQQLCTTIKTTLLEKALTIHELSAKYNHQKEKIIKTTRWMLDQGEIYCDD
                     TLKLHLNKTK"
     gene            12865..13776
                     /locus_tag="PLJ82_00760"
     CDS             12865..13776
                     /locus_tag="PLJ82_00760"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOR40074.1"
                     /translation="MKAELTILGCGSAMPSYGRFSASQVLSVRDKSFMIDCADGTLFR
                     MREIAIKTNRLNHIFISHLHGDHCFGLMAVLSTLGLMKRTADIYIHAHPDLENLLQPH
                     LRYFCNDLTYKIFFVPYNPLQSEIIHEDKSLTVTTIPLRHGIPTCGFLFKEKPQQLHI
                     KRDMIDFYQVGIKDLNAIKDGKDYITNQGETIPNSFFTTPASPQKSYAYCCDTAYSEK
                     IIPIIEGVDCLYHDATFAENEVLRAKKTLHSTAKQAATIASKAQVKQLLLGHFSARYP
                     HVSVILNEAQTIFANSIIAEDKKTYTF"
     gene            13902..15377
                     /gene="guaB"
                     /locus_tag="PLJ82_00765"
     CDS             13902..15377
                     /gene="guaB"
                     /locus_tag="PLJ82_00765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOR40075.1"
                     /translation="MSFIADKIAFDGLTFDDVLLIPAFSEVLPREVNLSTKFSRNIEL
                     KIPFVTAAMDTVTEAKMAIAIAREGGIGVIHKNMSIEEQARQVAIVKRAENGMIYDPV
                     TIKRGSTVADALAIMAEYKIGGIPVVDDNKILVGIVTNRDLRFERNLDRFIDEVMTKE
                     NLVTTKQSTDLESAAQILQQYKIEKLPVIDNNGKLIGLVTYKDITKAKDKPMACKDTK
                     GRLRVAAGVGVTDDTLDRMQALVDAGVDAIVIDTAHGHSISVIEKLKEAKKRFPQIDI
                     VVGNIATGEAAKTLVAAGADAVKVGIGPGSICTTRVVAGVGVPQLTAVYDVAKALKGT
                     GIPLIADGGLRYSGDVVKALAAGGFSVMIGSLVAGTDESPGETIIFNGRKFKTYRGMG
                     SLEAMEKGSKDRYFQSGEFDVKKLVPEGIAARVPYKGSLYEVIFQMVGGLRAGMGYCG
                     ARDIEELHNAKFTRITAAGVKENHPHDVTITHEAPNYTRGE"
     gene            15394..17331
                     /locus_tag="PLJ82_00770"
     CDS             15394..17331
                     /locus_tag="PLJ82_00770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR40076.1"
                     /translation="MKKWIVFIFVSLSVSYTLAQKNTIVTIDQKPISIEEFEYYYNKN
                     NTNDLQQKMPVEEYMQLFVNFKLKVHQAYMLQMDTAQTFKQELEGYRNQLIKPYLTDN
                     EKIDDLVKEAYQHLLEDVDVSHILIRLNANPTPADTIAAYTKAMTAYKRVTNGESFEK
                     IAVEFSEDPTVQENKGRLGFITGMMVVYPFEKTAYTTPVGQVSAPIRTRFGYHIIKVH
                     DKRKTQGERLVAHILKRIPEDASEKQKAAIERSIKSIYEDLAKGADFQRLAQANSDDQ
                     SSANKGGEISWVGTGKTTPEFENAVFNLQNIGDISQPVKTEFGWHIIKLLDKRDIMTL
                     QEAYPLIEPRVKTDERASIIANSFIEKLKKTYNFKSYPANLHALHTLLAQPNDNILEQ
                     TKTYNLPIYTFADQVITQSDLIVFLHQQNYTKNPIDLSLLDILANQVANNTIIKYENS
                     MLEKKYPEFGLLMNEYKEGILLFNISNEMVWSKASKDIEGLTDFFEKNKKSYAWDKPR
                     FKGAIIYCDSKQSFKTAKKIAKATPEKELSTKLKQTLNSNEKNAVKIETGLFAEQTNP
                     VVDKLVFKKGKLEKNNDYPYIFTSGNIQKHYPDSYQDIRGIVINDYQNYLDKQWIEEL
                     RSKYPIVIDTSVLNSLKSNNE"
     gene            17324..18187
                     /locus_tag="PLJ82_00775"
     CDS             17324..18187
                     /locus_tag="PLJ82_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40077.1"
                     /translation="MNKRYSFYLILITSTLLGCNKVNITVDSDRVPVMSVNNEVLYAD
                     EIENILPANITGKDSVNFVENYKKNWAINILLYEKAEQNLRSTEEIDLLTEEYRKQLI
                     INRYQQELISQKLKNISNDTLINVYEREKTNFILEDPIIKGVFVQIPSKAPNQKQLKK
                     WLTEMNDNTLEKIEKYCIQYASRYIFFRDDWTPYSQVDKLFPHHVDWNDPILTRGLVE
                     QKDSISTYFLRITGKANPGTIEPFEIALPKITNILSQGEKMNYIKNFENSLYNNAIKK
                     QKITFFLSSEE"
     gene            18199..19554
                     /locus_tag="PLJ82_00780"
     CDS             18199..19554
                     /locus_tag="PLJ82_00780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR40078.1"
                     /translation="MLKKITLLFVVCIFSIGVFSQNYIVDQVVWIVGDEAILQSEIET
                     EILRLKYEKQKTPESPECVIAEQLALQKLFLAQAKIDSITISEATINQQVESRIAYFT
                     EQLGSKEKVEAYFKKPLSKMREELKAMLQDQLMVQEMQQKITKDIKITPSEVTAFYEN
                     IPKDSLPIIPEQVEIQLITLSPTISVAEVERVKSKLREFRERIETGDIQFSTLAILYS
                     EDRVSALQGGEIGFRSRSMLVPEYANTAFSLTDPQKVSRIVETEYGFHIIQLIAKQDD
                     QVNSRHILMRPKISLEDKNLASQRLDSIAQAIRTGSINFEKGVEIYSDKSSTKNSNGL
                     LINTKDGSTRFQYDELPQEIINQVSFLNIGEISKPFSFTNENGKEIVAIVKIHDKIKA
                     HKASISDDYQTLKMVYTNVKNTQAIQQWIESKIATTYNQLTPAYRTCNFSYPHWLKNR
                     E"
     gene            19610..21133
                     /locus_tag="PLJ82_00785"
     CDS             19610..21133
                     /locus_tag="PLJ82_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOR40079.1"
                     /translation="MKNNVSIIVFLYVMCIALCYNANVTAQNKTTVFLENAETLSFDQ
                     NVRNNAQLLQGNVRFRHDKTLMLCDNAYFYEKTNSLDAFGNVKIIQGDTLFIYGNTLY
                     YNGNTRLAQMRNNVRMINRDAVLTTQKLDYDTYSNIGYYTDFGTLTDKKNVLTSKEGE
                     YLTKQNIIKFKKNVVLKNAEFTLYSDTLHYHTYNSLATIVGPTTIISDSTRIYAEKGW
                     YNTSTEDTKLTKNGKIQHKNGKSIQGDSIFYNKKYATIEAFSNTLLKDSVQQISVLGN
                     YGKYFEQNEKGYVTKKATFREHSSADTLFLSADSLLYESIKGDFSVKAFRNVRFWQTD
                     FQGTCDSLTYNQTDSLLTMYHNPILWNEENQISGKVITLKMTGDKVDHFIVEEDAFIL
                     SEENPEEFNQLSGRKIIGYILNDKIRKIEIIGNAQSIYYVKDGESAIGTNRAESALLT
                     IFLNDSNQAEKIVMSPQSGGILYPTFQKNISIIRLSNFLKQNNNRPKNQYDIYTNRE"
     gene            complement(21173..22525)
                     /locus_tag="PLJ82_00790"
     CDS             complement(21173..22525)
                     /locus_tag="PLJ82_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOR40080.1"
                     /translation="MKTTKKIVLNENDMPREWYNIVADMHTKPMPPLHPVTKQPLAPA
                     DLEPIFAKELIKQEVSSERWIEIPEEVRDLYRIYRPTPLVRASGLEKALDTPAKIYFK
                     NESVSPVGSYKLNSAIPQAYYNKIEGITSITTETGAGQWGSAMSVACQHFGINLQVFM
                     VKISYEQKPYRKSVMNTYGANVVASPSNLTEVGRRMQQQFPGTNGSLGMAISEAMELA
                     MKDEKTKYTLGSVLNHVTLHQTIIGLEAEKQMEIAGDYPDVVIGCFGGGSNFSGIAFP
                     FLRHKLTEGKDIRLIAVEPASCPKLTRGKFLYDYGDTEGFTPLLPMYTLGHNFEPAEI
                     HAGGLRYHGAGTLVSQLRKDGLIEAEAIKQNETFEAGVLFARAEGIVPAPESTHAIAM
                     TIREALKAKEEGKERVILFNLSGHGLIDMAAYDKYLSGSLSNYEVSDAEIEKSLAGLK
                     "
     gene            complement(22865..23878)
                     /locus_tag="PLJ82_00795"
     CDS             complement(22865..23878)
                     /locus_tag="PLJ82_00795"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOR40081.1"
                     /translation="MEKKIKAVINGIGGYVPEAILDNEELSRMVDTSDEWIMSRIGIK
                     TRHVLGERRGSAYMGIKAVQDLIEKTSLDTKDVDLLICCTTTPSFVFPSNASLICGAL
                     GITSAYGYDLAAACSGFIFGLDTCATMIESGKYKKIILVCSEKMTAITNYEDRNTCPL
                     FGDGAAAVLIEPTTEDVGVVDSILRIDGLGKENLHLKAGGSLHPASIETVTNKEHFIY
                     QEGQAVFKRAVSDMSAVAEEIMIKNALTSDDIAWLVPHQANIRIIEAVAKRMSLPREK
                     TLINIEHYGNTSAASIPLCLWDYESRLRKGDILVLAAFGAGFSWGSVLLKWGYDIEHS
                     KKM"
     gene            complement(23964..24149)
                     /gene="rpmF"
                     /locus_tag="PLJ82_00800"
     CDS             complement(23964..24149)
                     /gene="rpmF"
                     /locus_tag="PLJ82_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOR40082.1"
                     /translation="MAHPKRKQSKTRTAKRRTHDKAVPATMAVCSNCGAMHIYHTICG
                     ECGYYRGKLAIEKTVNA"
     gene            complement(24179..24688)
                     /locus_tag="PLJ82_00805"
     CDS             complement(24179..24688)
                     /locus_tag="PLJ82_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOR40083.1"
                     /translation="MSHYIIPLKSIVSYNTTYDYELDESFFSAFVAEDGIIRGGNVKA
                     SIDVKKLNYSFELSIKLNGFVSVLCDRCLDVMEIQIDEDSVLVVKFGETYTEVDEELV
                     IVPEKEGVFNIAWYLYESLVLILPLQRIHQDGGCNKEMEKNLQKYSTDITVVNEKIDP
                     RWEALKNIK"
     gene            complement(24853..25998)
                     /locus_tag="PLJ82_00810"
     CDS             complement(24853..25998)
                     /locus_tag="PLJ82_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR40084.1"
                     /translation="MTSIYEVRQRFKLLFSSLAILIAISSFVVTNSLVQKLSDEEKSK
                     MQIWANATKELINMDEYCDISFVQQVIEANTTIPVILVTQEDSVISYKNIPERLQESR
                     KLQQLVEEYKKSNTPIEIVFSDTERQYLYYGNSLLLIQLSYFPFVQLIAVFIFLVFVI
                     WALLSTKKAEQNEVWVGLSKETAHQLGTPISSLMAWTEIFKSKYIEDSMFGEMEKDVN
                     RLRTIAERFSQIGSKTDLEIVNLNQVIHNAVDYLRTRISKKITLSIQENEVLYCELNV
                     SLFEWVIENLCKNAADAMSGDGIIKIQLSQKGKEIRIDVIDNGKGISKHNSRKIFNPG
                     FTTKKRGWGLGLSLAKRIIEEYHQGKIFVQHTEIDKGTTFRIIFYVQ"
     gene            complement(26076..26999)
                     /gene="rsgA"
                     /locus_tag="PLJ82_00815"
     CDS             complement(26076..26999)
                     /gene="rsgA"
                     /locus_tag="PLJ82_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOR40085.1"
                     /translation="MQGLVIQNNGNIYLVQLQTGVSVECRIKGNFRLKGIRSTNPIAI
                     GDNVLVDLQTNGHSFIYDICDRKNYIVRKSSNLSKHSHIIGANIDQAILVVTVNYPET
                     STGFIDRFLVSADAYHVPVIIVFNKVDLYIENERNYLDALKQLYQHIGYRCIETSVVS
                     MQGIEELKECLRGNISLFSGNSGVGKSSLIHQIDPTLDVKIGLISEMHNKGMHTTTNS
                     HMYTLSGGYIIDTPGIKGFGIVDIKKHEVAHYFRDIFEYSHQCKYNNCLHQQEPDCAV
                     IKAVGEFRISQSRYQSYLSILQDMTEDAKYR"
     gene            complement(27009..28154)
                     /locus_tag="PLJ82_00820"
     CDS             complement(27009..28154)
                     /locus_tag="PLJ82_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40086.1"
                     /translation="MKFVIYVIIFFYVMLTNGYAISEVSEIVAAKDSLHSDTATVMPS
                     CPLIKESWFTIPMGYYQQETSVALGIVGGYYFPSTNLRKISSISGSVIYTFRNQFIIN
                     ANPRFYGGDSNWFFNGNVNLRYYPTYYYGIGNSNSIYNESYISKSFQLMLQSGYFIND
                     NWQIGPYVSLRGEKITVGESFENHIEAIFARYGSAGWNPYWMTGLGVFSTYDTRDNVF
                     YPQKTSVFIKTSVLNYFSILGSTFDVASFNGDFRCFLPTWFGQLIACQLYLDARLGGN
                     IPFQLLPTVGGSDNLRGFRERKYIDDMFVQLQTEYRIPIWNRLKATLFYSVGDVVSTD
                     NPSFRLKMSYGGGLRFRLNDARVHLRADIARTNYGNVEFYITATEAF"
     gene            complement(28252..28809)
                     /gene="frr"
                     /locus_tag="PLJ82_00825"
     CDS             complement(28252..28809)
                     /gene="frr"
                     /locus_tag="PLJ82_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOR40087.1"
                     /translation="METKQYLTAAIEKMETTLLFLDDALAHIRAGKANVRILDAVRVD
                     YYGSMVPLSNVATLTTPDARTIAIQPWEKGLIKEIEKAILHSDVGITPENNGEMIRLG
                     IPPLTEERRKMLVKQSKQEGEEAKISVRNVRRDVIDAIKKAVKDGLAEDMAKDAETEI
                     QKIHDKFIKKIDDAVFAKEKEIMTI"
BASE COUNT        10073 a         5532 c         4309 g         8986 t
ORIGIN      
        1 gatagaagac cgcacatcgt taagtgcctc atctaaattg gcaccccact caaattcaag
       61 cgtaaccaag gataaattat ctttagatgt ggaatacacc tcttttaatc catctacaga
      121 attaaggcta ttctcaagaa tcttactcac attggtctcc acctctgtgc cgttagctcc
      181 aggatacgaa gtcataaccg ttacagatgg gatttcaatt tctgggaatt gatcaatagg
      241 caattgtatt aacgaataca agcccatgat aattaccccc acaaatatca atatagatgt
      301 gatgggttta tttacagctg ttttatatat actcatggtg ttgtgtattt ctaatcgttt
      361 acaatagtta attgtacgcc atttactaag cgcgcttgac ccactaccac taattggtct
      421 ccctctttga gttcatcaga taaaatttct attttatcat catagcgttt ccccatttgt
      481 acttttacac gtttcgctac tccattctta ttaacatata catagcgttc gttagagcct
      541 tgtagtttta agactgcttg ataaggtact actttagcat taattttatc aaaatcaact
      601 ccaatacgtg caaacattcc tggtcgtaat actccgccag cattcgagat tttaatttgc
      661 acctgaaaag tatgtgtagt agcgtctatc acaggcgaaa caatttctat tattcccttg
      721 aattctttgt cagcaaacac atccgatgta attgccacgt tcataccttc tttaatggat
      781 ggatagtaac tttcgggaat attaatatgt gctttcaaat tggccatatc tactaaaagt
      841 aaaataggtg ttccagcata taattctcca ttttcataat ttttagcagc tataacaccc
      901 gagaatggtg ctcttaaaaa ggtattccgt tctaaattct gaacatttgt ttttagtgat
      961 tcgtattgag cctttgtttg atcatacgtt tgctgtgcta tattacctga attatacaac
     1021 gtggaaatac gttgaaaatc gactgctaaa ttatcaaaac tcacttttgt ttgcaagtaa
     1081 gtagttttgt ccatttctac taacaattga ttggcttgca ccttagaacc aacttctaca
     1141 tgtattttat caatacgccc agccatagta ggggcaacca ttaccttatt gtaaggctct
     1201 aatgtggttg aaaaatcaat ttttcgagct atttcttgcg tttcaagaac agctgtttta
     1261 acattttcga cacgaatttc ttcttcctgt tttttttctg ctttaggcgt gcatgcattc
     1321 agtaaagcaa ttacagccaa catgagcatt gatattttta ttcctttttt catcttggta
     1381 actattaaga ttggtttagt ttaaagtttg tttattaatt gttgtaattt agtttttgcc
     1441 attaacatat ccattaaagc actgatatat tgactttgag tagataatgt attattgctg
     1501 atagttgaca attcagtact cgaaatcatt ccctgctcgt atttttgagt agcatttgtt
     1561 aaaatacgtt cgtataaagc cacgtttttt agttgtaatt catacgattc aatagctgaa
     1621 gtcaaattat aacgcaactg tttttcttcc attaacaagg catccactgt ttgttctttg
     1681 tttaatttag cagtttgcaa atcaaattga gcttgtttta ctttggcata acgttcaccg
     1741 cttgaaaaca caggaatact aatggtaact cctaccaaat taggtggagt catatcaaag
     1801 ccaaccgctt tatcaaaatg tgttttcttc gtatattgat aaaaaccagt gagcatcggt
     1861 gcgtacgccc attggtttaa tcgaacttgc ttttgattca tttcgatagt ttgttgcaac
     1921 agtgtaaccg tataattttg tgtcatatca aacgatgtag ccaatagttc attggcagat
     1981 tctttgtttg taaaatcttt tagcgtattt ttcaatgaaa gtggcgtgtt attatcaaca
     2041 cccaataaca gcctcaaggt attgtatgat aattctacat tcctttctgc catacgtatt
     2101 gtattttcaa tcatacctac ttgcactgct aattggtcag catctgtttg ttctgccatg
     2161 cccactttta caagattaag cgaagaattg tatagtttgc gtaaattttc taaattacct
     2221 tcaactattt tttttgattc ttcagacact aatatggcat aatacgcatt ggttacattt
     2281 gtttgcaagc ttaatacatc cgacaacgca gaagtttgcg acatttgttg agctatctcg
     2341 ctcagctgca ctcctactac ttgcatacca ctgagtgcaa tggaagcctg agcggtaaaa
     2401 ctcccctcgg gattcatagc aattggtgta cccattaatg acattttatg acccagataa
     2461 ttattatagc ccaatgatgc atccactttt ggtagcaaac tagcaataga ctgccatttg
     2521 gcataatcag ctttttgaat ggctaaattg gctttttgaa ttgttttatt atagtttaga
     2581 gcgtgctctt ttgcctcatc aatgcttata tgcaaagatt cttgagctat actatgcacc
     2641 gaatagccaa gaaagaatga ggcgaacaat agaaataatt ttcttttcat ctgatatgtt
     2701 tttatttttt tatgttaata atgatgtata atacagtaat cctttttcgt tgcacaccat
     2761 acgcaaataa ctatcaacca taaaatcaat gatgccacgt ttggtgtatt tgcgaggatg
     2821 cttaatggac atattaataa aatattgcaa ggaaacagtt aacgactgag ccgtgaattg
     2881 aacatgaata tcttctctaa aaacaccttg tgctattcct ttttctatct gaataagaaa
     2941 catttgttct gtatattgtc gtttttctac agaaaaggta ttgtatattg aaggataata
     3001 ttttagcaaa tcattaaaaa atctttcatg ttttttttcc tctttgccat tttttatcaa
     3061 tgttttcatc cacaaaatct catcaatcac attcgtaaac tcttggtttt gaagcataca
     3121 atgatactct tggtgatgtg tggtaaacgt ttccataaca gccattacta atgcttcttt
     3181 ttgcctgtaa taactataaa aggttttttt agaaatacgt aaattatcac aaatatcatc
     3241 tacgctcaca cttctaatac cttttttaac aaaagcattg gtagcagtct ttaagatttc
     3301 ttgtttaatt ttgtccatat actaaaaata catttgtctc tttcactctg aaataggacg
     3361 caaagataga atacgaacaa taaattacca caaacattaa tgttaaatta ttgtaataaa
     3421 ccattaaaca ctgattatca aacaattaat ggaaacgaaa tattggttac aatttccaag
     3481 tttccatttc gaagaacaaa aattggataa aaaacaacac ttttagagtg aaacctcatt
     3541 aggaattgaa atttaaatca ttagtatgat aaattatacc aaatttgaca ataaacttag
     3601 gaaagaaaaa ttctattctg cagataaaac aattggcaac aacacaccat tactagcgtt
     3661 gggcaaagga atctgtaacc atttggcaag tgttgctgta gcatctatta tggcatacgg
     3721 tgtagcgatg gtttgtggct gtatcttcca tccataaaaa taaaaaggaa cactattgcc
     3781 taatacatta tagaaaccag ttgggttatc gtttttatct acctcaaccc aacctggata
     3841 taaggtaaat aatatatctc ctgagctttg cttgttgtag gattgtcgaa gcgaatttct
     3901 taacgcaaat tgttgctcca aagcgactga attaaccaca ttcatcacac caggcaattg
     3961 tgatacaaac tccatcgctc gttcttgcac ttcttttaaa ctcaaattct tttcgtctat
     4021 tatttttcta tttaaataaa tattcccgtt gtaaaaaccc attacccaac tttcctgacc
     4081 gtatatcgcc attagataaa cattcaacaa agccatatag cgatacgagt taaatgtgcc
     4141 gagttgcatt ttttctttta ataaaggaga cacagcataa tgagttttgc gaggagaggt
     4201 gatatataca aggatatgtt ctttgccaat ttttctttct actgcttcaa gaatcatttt
     4261 gatatcatta tctaatcgta catatgcgtc ttctaattcg acagttaaat cgcttgcttg
     4321 aggataggat tgtaaacgaa aattaagcaa caataaatca gggaaaatat ccctccccaa
     4381 agtttcttgc tctatagctt ctacagccat ttcgcgaata caggtattgg caaaaggagt
     4441 tttcttaaat tctttaaccc cattttttcc cattgtcatt tttttcaacg gataagaaaa
     4501 actttttgtg gtaaaccccc ttgattgtgt tacataataa taattaattg gatacaaggg
     4561 ttcccacacc atttccatat agttttgtat ggcattttgt tcgttcttgc gatttaccca
     4621 agatggcaac tgttcattgt aatacgtaga gcttgaccat ttaccagtgg tatcatcaat
     4681 ccacattact acatctgcca aatgtccagt ttgtaatata ccctctttag cattcattgc
     4741 tatagacact acctttgatc gctgaattga atgtattttc aattcatcag ctaaagtggt
     4801 aactaacaag ccccgtgccg aaacttgatc cactccatta attgatttaa cggcatcatc
     4861 ttgtaataaa aattggtact tacgggtatt tctgttatat acatcagacc aactaacacc
     4921 atgcaaataa ggctgagaac ctgtactaaa ggtagctata tcagacacat tatcagtagc
     4981 atcatagtca aactgaagag atgataatgt agctccattt tcaactatct tacgaaagcc
     5041 ttggtttccc attccgttcc aaaacatgga taaaaaatca gcagttaatc cctctacagt
     5101 aatcccaata accaaccgag gcttttgata atcggcttgt tgtgccaaca gtacaattgg
     5161 gaaacacaca aaacagacaa gatatttcaa tttcatacaa ttattatttt actacatgta
     5221 tagaaagaca tgttctttga acaaattact cttcagagaa atcaactaac acattgcgat
     5281 acgcactaaa aatagtagat tttgaaacaa aaccaacata tttatctcca tccaatactg
     5341 gcaaattcca tgcttgtgtt ttttcaaact tcatcattac ttcttccatc tcttctccta
     5401 caaaaatacg agatggcgga gcaatcatca aatcagcaac agtaaaacgt tgatacaatt
     5461 cggttcggaa cataatattg cgtatctctt ctaataaaac gactcctaaa aaaacaccat
     5521 ctttatctac tactggaaat aaattacgct tagaggtagc tattactttt actaaatcac
     5581 ccaaagtagc cgatggctca actttataca aatcagtttc gactaaacgg ttcatattaa
     5641 gcatattcaa tacagcttta tccttatgat gtgtacgcaa ctcacctttt ttagctaaac
     5701 gcatagcata aatactatgg ggttcaaaac caataatggt aagaaaagac acggttgaaa
     5761 caatcataat tgggataaat aattcgtatc cacctgacaa ttctgcaatt aaaaaaatgc
     5821 ctgttaaagg agcatgcatt actcccgata taattccagc cattcctgct aaagcaaaat
     5881 tttgcactgg caacacttct ccaataaacc ctgcaaccaa agcaaatgca aaaccagtta
     5941 tgcaaccaat gaataatgat ggagcaaaaa ttccacccac accaccagat ccgttggtag
     6001 cagaagcagc aaataccttg agcaaaacga taaatcccat atacatgacc acccaaatca
     6061 tattatcttt cagagtataa aataaactac cctcaaacaa tatatttggc tgattgttaa
     6121 gtaaagcaga aattgaatca tacccctctc catacaacgg aggaaataca aaaatcaaca
     6181 aactcaagac aactccacca acaaacaatt tagcatacat actttttagt cgctgaaaaa
     6241 aaccttccat tttgttcatt gcacgagtaa aatacaaaga caccaatcca caaactatcc
     6301 caagcagtat gtagtacgga attcgctcta ataaaaaagg ttcgtaacta tctgcggcaa
     6361 acataaccgt attacccatt aaaaaataag aaattgaggt tgcagttaca gacgatatta
     6421 acaatggcac cacagatgtc attgtcatat ctaacatcaa tacttctaac gtaaaaacaa
     6481 ctccagctat ggggcactta aaaatacctc cgatagctcc tgctgctcca catccaacga
     6541 gtaacattaa ggtcttttgg tccattttaa aaaattttcc taaattagaa ccaatcgaag
     6601 atcctgtaag aacaataggt gcctctgctc caactgaacc tccaaatcca attgtgatag
     6661 aactagccac aagagatgtc caattattat gaggtttgag tctactctta cgtttggata
     6721 tggcgtacaa aatacgtgtt acgccatgac tgatatcatc cttcactaca taacgcacaa
     6781 aaagtgtagt aataaaaata ccaatgatag gatacgctag atacaaataa ttctcatgat
     6841 taacactaaa attagtggtt aatatatgtt ggataaaatg tattgcactt ttcagaaaaa
     6901 aagcagctaa agctgtaaaa attcctacca aaaaactcaa aacaaggata aagttactcg
     6961 ttttaatttt tctttctcgc catttaagca acgataaata ccacaaattt acaatgtctg
     7021 tttgtttcat atctttcctt attcgtcaaa tccctcttcc tgttacaatt gtttttatcg
     7081 tataaatcaa tatcttacta tctattacta acgacatatt ctcaatataa gagatatcaa
     7141 attgagccct ttcaatcatt ttttctatgg tgttggcata accatatttt accatgcccc
     7201 aggatgtaat acctggtcgc acagtgaaca aacggtaata atacggagcg tgttcgataa
     7261 tttggtctac aaaatatcta cgttctggac gaggtcctac aatagccatt tctcctttca
     7321 gaacattata aaattgagga aactcatcaa ttctatattt acgcataaaa cgtccaaatg
     7381 gagtaatccg ttcatcattt tcacaagata aacgtggtac accatcctgt tctgcatttt
     7441 catacatggt tctaaactta tacatcataa aaggaattcc atgtaaacct acacgctctt
     7501 gcttatacaa tacttcccct tttgaatcta atttaatgcg taaagcaata attacaaaaa
     7561 atggcaatcc aatcaacaaa accatactag aaaggaggat atcaaataag cgtttcaaat
     7621 tgagctgaaa atcgctcatt tgtatttcta gtaaatcaat taaaggtaca gcataaatac
     7681 tattcatttt cacactacca gttaatattt caaacaaact tggtacaatt tttattttaa
     7741 tatttttcga ccacaacgac tgcatcaatt gataaatatc atcttcagta atactatcaa
     7801 cagcaataat aactacatca acaggatatt tctttaaaac gttattgata tccacaatag
     7861 agccaactac actgctatta ttaacttttc ttgtttcatt tttttgtaat gccacaaagc
     7921 cttctattat gtaacctaaa gattggttca tttccgaaag ttgcttttct aatttaaaag
     7981 ctttttctcc tacacccaat accaatgtcc gtaaacctat ttttcgcgta tgaataagat
     8041 gggctgattg tgtagtaata agtaaccgaa aggcatatgt tagactaaaa tggagtataa
     8101 aataaacaaa aaaagaaata tagtaaaatg tgtatgaggc aacaggatca tccatcaata
     8161 cagaaaaaaa cacaatcaag ctaccaaaaa aaacactaag aattgtagtg aagaattctg
     8221 acaatcgcga tttaaaaaac ggagtattat aatatcctga tactacgtat aaaactatcc
     8281 aaaagaaagg gacaagtaac atagtaacac gtgcattgta catgggtaca aacatttgaa
     8341 tatcccgaat agccactaaa tcaatttcaa tacgccgaaa tacatagaat aaaaaccacg
     8401 ctaatacagc tgttatgtag tctgcgagaa tatatttgat tacttgcttt ttgtggttca
     8461 ctatatataa tctaattaaa ttactttcgc aaaattacaa aagttccgtc tttttgcaat
     8521 tttattattc catatcctgc atataatttt cgatttttgt caaataccat attgaaatac
     8581 ggaataatat gttgtatttc tgcaatttct ttatcggttt ctgaaacaaa agggataaat
     8641 aataaaggaa ttctaaattg ttgtaataaa ttttgaagaa atccatctga aagtacacca
     8701 agagcaacat tagcactatt ttgttgaatg gttgcactta cattttgcaa ttcagaaaaa
     8761 acaattaata cattattttc tgatgtttcc cacaagtcat acgctatttc aggtatctca
     8821 gacacataat gccttaattt aacgacatta tctaccaata aatacattcg ttggggtaat
     8881 agtttggtat acattttatg tactaaattt tcgttcgtag catcacaaag cacaccatat
     8941 ttattatcaa aataacaaaa agctatatga gcatttctca attcatctac gtactgttta
     9001 accacaatat tattctgctt tatcattctt tacattcaag tcgaaaaata tcttgtaaac
     9061 attttttcac actcacaaga agttgttttt actattttat tgtatcttta cgatttgatt
     9121 tattcttttt actttaaaaa agtgaaaatc gaattatgcg tacaaatata caatgatttt
     9181 gtacagtttt tatcacagcg ataataaaaa cacactatat gacaaacaat tggattgtaa
     9241 aagagcttac aacggaagaa tctgaacaga ctgaacattt aattaaggat ttggggataa
     9301 gtccaatttt gtgcaaacta ctcgtacaaa gaggcataca cacaatggaa gaagcaaaaa
     9361 actttttccg acctcaatta agtcagctac acgacccttt cttactcaac gacatggaca
     9421 aagcagtagc acgcttaaat aaagcaatga gaaagaaaga aaaaatcttg atatatggcg
     9481 attacgatgt agatggtaca acagcagtag ctgtggtata taaattttta caacagtttt
     9541 attcaagtat tgattcgtat atcccagata gatacgagga aggctacggc atttcataca
     9601 aaggaattga ttatgctgca gaaaatggtt tttctctgtt aattgtactt gactgtggca
     9661 ttaaggctat agagaaagtg cgttatgcca aagaaaaggg gattgatatt attatttgcg
     9721 accaccatac tcctgacgaa attcttcctg atgctattgc ggtattagac cctaaaagag
     9781 ttgattctac gtatccatat cctcatttat ctggttgcgg tgttgggttt aaactcatac
     9841 aagcttttgc tataaataat caaattgact ttgacaacat tacccctttt cttgatttat
     9901 tagccgttag tattgcatct gacattgtcc ccattacagg cgaaaataga atcttgatgt
     9961 tttttggctt aaaacaacta aacgcgaatc catgtgttgg gctacaagcc attattgatg
    10021 tatgtggctt gagaggcaaa gaaatcacta cttctgatat cgtttttaaa ataggtccac
    10081 gtattaatgc atcaggcaga atgagatctg gcaacgaagc agtagagtta ttagttagta
    10141 aaaatctaag ctttgctaag gctaaaagcg actgcataga ccaatacaat caagaacgca
    10201 aagatttgga caaaaaaaca acagatgacg ctaaagactt catccaaggg aacgaagatt
    10261 ttgaacacag aaactccatc gtcgtttatc atcctgaatg gagtaaagga gttattggga
    10321 ttgtggcatc taggctgagc gaattatacc acaaacctac tattgttctt acgctttcaa
    10381 atggttttgc tactggttct gcacgttctg ttactggttt tgatatttat aaagcaatag
    10441 aatcgtgtcg ggatttgtta gaaaactttg gaggacatat gtatgctgct ggattgacca
    10501 tgaaagaaga caatgtgtca atttttacag aacgttttga taactatgtc cgcgaaaata
    10561 ttaccgaaga acaaacacaa cctcaaatag atattgacgc cattatccgt tttagtgata
    10621 taacgccaaa attttttaat atattacaac aatttgcacc atacggtcct gaaaacatga
    10681 aaccaatctt tgcaacccat aaagttacag attttggctc cagccgacta gtaggaaaag
    10741 aattagaaca tctaaaacta gaactaactg agcccaactc agaaaatgta atgaatggaa
    10801 ttgcttttgg gatgggagaa aaatttagtg ctcacattaa agacctcaaa ccaatcgata
    10861 tttgttatac gttggaagaa aacgtcttta atggaaataa aaccatccaa ctaatgataa
    10921 aggatatgaa aattaatgaa taaatagcag ttaataacct aacatgtacc acgaaatttt
    10981 acatacatat tggggatatt ctgaattccg ttcattacaa cttgatatta ttcagtctat
    11041 tggaaacggc aaagacacct tgggcttaat gccaactggt ggaggaaaat cgcttacttt
    11101 tcaagtacca gctctagcaa aagaaggttt gtgtttggtc attactcctc ttattgcttt
    11161 gatgaaagac caagtagaaa acctgaagaa gagaggaatc aaagcaactg ccatatacag
    11221 tggcatgagc tatgaagaaa tgatgattgc tatagataat tgcatgtatg ggaattataa
    11281 gtttttatac gtttctcccg aacgactttc aactcgagct tttttactta aaatacctca
    11341 tctacaaatc aatcttattg ccgttgacga atctcattgc atttcacaat ggggatacga
    11401 ttttcgtcct tcatatcgtc aaatagcatc tattagagaa catttaccta cagtacctat
    11461 tttagctctt acggctacag caacccccga agtagtacgc gacattcaaa cacaactcaa
    11521 atttgctgca ccaaatgtgt ttcaaaaaag ttttgttcgg agcaatttaa tttatattgt
    11581 acgtcaaacc gatgataaac tgcaacaatt actacaaata cttcaaaaag tgcctggtac
    11641 ttctgttgta tatgtaagaa acagaaaaaa aacaaaagaa atagctgatt ttttaaacga
    11701 aaacaatatc cctgctaatt atttccacgc aggacttagt aataatacca aagatgaacg
    11761 ccaacaaaaa tggaaaaatg atgaatgtcg tgtaatggta tctaccaacg catttggtat
    11821 gggcatagat aaaccagagg tacgtactgt tatccattta gacttaccag atagcattga
    11881 aaactatttt caagaagctg gtagagctgg tagagacgaa aaaaaggcgt atgccatact
    11941 actgtatagt aatgctgata aagtaaaaat tgaaaaaaga atacgagaca acttccctga
    12001 aaaagaaaaa attaaagaaa tttacgaagc catatccaat tacctgcaaa tacccgtaga
    12061 aggaaaagag ggagctgtat atgattttga gatatttgat ttttgtgcca aattccactt
    12121 tccagcttta cttgttcata gtgcaatcaa tattctagaa caagcagaat atatatgcta
    12181 tacagaagaa gtagacagta attcgcgcat tctatttact gtttcgcgtg atgatttgta
    12241 tgcaattaac aactcccata tacattcaga aaaaataatc catgctcttt tacgctcata
    12301 cacaggacta tttacggatt atgcatacat caacgaagag ataattgcac aacgtgctgg
    12361 attaacagta gacgaaacaa ttcagacact aatctttcta gctaaaacta aagttattca
    12421 atatattcct cgaaaaaaaa caccttttat tacctacacc caacaacgac aagaaaaacg
    12481 atatctaaaa attccgagag aagtatacga agaaaaaaaa gaacgttatg taaagcgtat
    12541 cgaatcaatg attgcctacg ctacaaatac taccgtttgc cgaagccgaa tgcttttgca
    12601 ttattttggc gaaagcaaca cacatgattg tgatcagtgt gatatttgct tgacacgtaa
    12661 aaaaaatgat ttagccaacc aagaatttca acaactttgc actaccataa aaacaacttt
    12721 actagaaaaa gcactaacca tacacgaatt gtcagcaaaa tacaaccacc aaaaagaaaa
    12781 aatcattaaa actacacgat ggatgcttga ccaaggagag atatattgtg atgatacgtt
    12841 gaaattacat cttaataaaa caaaatgaaa gctgaattaa ccatattagg atgcggatct
    12901 gctatgccat cgtatggcag attttccgct tcacaggtgt tatctgtacg cgataaaagt
    12961 ttcatgattg attgtgctga tggcactctc tttagaatga gagaaatagc tatcaaaaca
    13021 aatcgtctca atcacatatt tatttcgcat ttacatggag accactgttt tgggcttatg
    13081 gctgtattat ctaccttagg cttgatgaaa agaaccgctg atatatatat tcatgcccac
    13141 cctgatttgg aaaatttgct acaaccccac ttacgctatt tttgtaacga tttaacatac
    13201 aaaatctttt ttgtacccta caatccgcta caaagcgaaa taatacacga agataaatca
    13261 ctaacagtaa caaccatccc attacgccat ggcataccta cttgtggatt tcttttcaaa
    13321 gaaaaacctc aacaactcca tatcaaacga gatatgattg atttttacca agtaggaata
    13381 aaagatttga atgccattaa agacggaaaa gattacataa caaaccaagg agaaacaata
    13441 ccaaactcct tttttacaac tccagccagc ccacaaaaaa gctatgctta ttgttgtgac
    13501 acagcatatt ctgaaaaaat aatacccata atagaagggg tagattgttt gtatcatgat
    13561 gctacttttg ccgaaaatga agtactcaga gcaaaaaaaa cattacattc tacagcaaaa
    13621 caagccgcaa caattgcctc aaaagcacaa gtaaaacaat tactattagg acatttctca
    13681 gctcgctatc cacatgtatc cgttatttta aacgaagctc aaacaatatt tgccaatagt
    13741 ataatagccg aagataaaaa aacatatact ttttagacaa aaaataacac ttactgtcca
    13801 aaaatacgcc tttagctaac taattttaac gtagatagaa taccgtaagt tcgatatttt
    13861 tttcgtatat ttgcacgcaa taattttaat acctaaatcg tatgtcgttt attgcagata
    13921 aaatagcttt tgatgggtta acatttgatg atgtactcct catccccgca ttttccgaag
    13981 ttcttccacg cgaagtaaat ctttctacaa agttttctag aaatattgaa ttaaaaattc
    14041 cttttgtaac agctgcaatg gataccgtaa ccgaggcaaa aatggctatt gcaattgctc
    14101 gagaaggagg tattggtgtt atccacaaaa acatgtcaat agaggagcaa gctcgacaag
    14161 tagccattgt taaacgtgcc gaaaacggta tgatttacga ccccgttacc attaaacgag
    14221 gatctactgt agctgatgcc cttgctatta tggcggaata caagattgga ggaatacccg
    14281 tagtagatga taacaaaatt ctagtaggca ttgttacaaa ccgcgattta cgttttgaac
    14341 gtaatcttga ccgttttatc gacgaagtaa tgacaaaaga aaatttggta actaccaaac
    14401 aatctaccga tcttgaatca gcagctcaaa tactacagca atataaaatt gaaaaattac
    14461 ctgtaattga taataatggc aaacttattg gattggttac atacaaagac ataactaaag
    14521 ccaaagacaa acctatggcg tgtaaagata ccaaaggacg attgcgtgtt gctgcaggcg
    14581 ttggagtaac tgacgatact ttagatagaa tgcaagccct agtagatgca ggggtagatg
    14641 ctattgttat tgatactgct cacggacact caatttctgt aattgaaaaa ctaaaagaag
    14701 caaaaaaacg ttttccccaa attgatattg tagtaggaaa tattgcaaca ggagaagctg
    14761 ctaaaacatt agtggctgct ggagccgacg ctgtaaaagt aggcatcggt ccaggttcca
    14821 tctgcacaac aagagtggta gctggcgtag gtgtacctca attaacagct gtatatgatg
    14881 tagccaaagc cctaaaagga acaggtatac ctttaattgc agacggagga cttcgctatt
    14941 caggagacgt tgtaaaagca ttagccgcag gaggtttttc tgtaatgata ggctccttgg
    15001 tcgcaggaac agacgaaagt ccaggtgaaa ccatcatatt taacggcaga aaatttaaaa
    15061 catacagagg aatgggttct ttagaagcaa tggaaaaagg atctaaggac cgctacttcc
    15121 aaagtggtga gtttgatgta aaaaaattgg taccagaagg cattgccgca cgagttcctt
    15181 ataaaggctc tttatacgag gtaatttttc agatggttgg tggattacgt gctggaatgg
    15241 gctattgtgg agctcgcgat atagaagaat tgcacaatgc taaatttaca cgtattactg
    15301 ctgctggagt aaaagaaaat caccctcacg atgtaactat cactcacgaa gcacctaatt
    15361 acactagagg agaataaact aaattttgat tgtatgaaaa aatggattgt gtttatattt
    15421 gtcagtttaa gtgttagcta caccctagct caaaaaaaca ctatagtaac tattgatcaa
    15481 aaacctattt caatagaaga atttgaatat tactataata aaaataatac aaatgatttg
    15541 cagcaaaaaa tgcctgttga agaatatatg caattgtttg taaactttaa actgaaagta
    15601 catcaagctt atatgttaca aatggataca gcccaaacat ttaaacaaga actagaagga
    15661 taccgtaatc aactaataaa accatactta acagataacg aaaaaattga tgatctcgta
    15721 aaagaagctt accaacattt actcgaagat gttgatgtta gccacatttt aatacgacta
    15781 aatgcaaacc caacccctgc agacaccatt gctgcataca caaaagcaat gaccgcatac
    15841 aaaagagtaa caaatggtga atcattcgaa aaaatcgctg tcgaattttc agaagaccct
    15901 accgtacaag aaaacaaagg aaggttaggc ttcattacag gcatgatggt tgtctatcct
    15961 tttgaaaaaa cagcatatac aactcctgtc ggtcaagtct cagcaccaat cagaacaagg
    16021 tttggatacc atattattaa agtacacgac aaaagaaaaa cacaaggaga acgattggtt
    16081 gcacatattt tgaaacgtat accagaagat gcatcagaaa aacaaaaagc agctattgaa
    16141 cgctcaataa agagtatata cgaagattta gccaaaggag ctgattttca acgtctagct
    16201 caagcaaact ccgacgacca gtcatctgcc aacaaaggtg gagaaatttc ttgggtagga
    16261 acaggaaaaa caactcccga atttgaaaat gctgtgttta atctccaaaa tattggagac
    16321 atctctcagc ccgtaaaaac agaatttggc tggcatatta ttaaattgct cgacaaaagg
    16381 gatattatga cattgcaaga agcatatccc ctaattgaac ctcgcgtgaa aactgatgaa
    16441 cgagcaagca ttattgccaa tagctttata gaaaaactta aaaaaacata caactttaaa
    16501 agttatcctg caaacctaca tgcattacat actctactgg cacaaccaaa cgataacata
    16561 ctcgaacaaa caaaaacata caacttgcca atttatacat ttgccgatca ggttataact
    16621 caatcagacc taattgtgtt cttacaccaa caaaactaca caaaaaatcc tattgacctt
    16681 tctctccttg atatattggc aaatcaagta gcaaataaca ctattatcaa gtacgaaaat
    16741 agtatgttag agaaaaaata ccctgaattc ggactcctca tgaatgaata taaagaaggt
    16801 attttattat ttaatattag caatgaaatg gtttggtcta aagcctcaaa agatatagag
    16861 ggactaactg atttttttga aaagaacaaa aaaagttatg cttgggataa accccgtttt
    16921 aaaggagcta ttatttactg cgactcaaaa caatctttta aaactgcaaa aaaaatagct
    16981 aaagcaaccc ccgaaaaaga actatcaaca aaacttaaac aaacattgaa ttcaaacgaa
    17041 aaaaatgctg taaaaatcga aacgggactt tttgctgaac aaacaaatcc agtcgtagac
    17101 aaacttgtat ttaaaaaggg gaaattagaa aaaaataacg attatcccta tatatttacc
    17161 agcgggaata tccaaaaaca ttatcctgat tcgtatcaag atatacgagg cattgtgata
    17221 aatgattacc aaaattatct cgataaacaa tggatagagg aactacgatc aaagtatccc
    17281 atagtaatag atactagcgt tttaaatagt ttaaagtcta ataatgaata aacgttattc
    17341 cttctatctc attcttatca cctccacact attgggatgt aataaggtga acattacagt
    17401 cgactcagat cgggtgcctg tcatgtctgt aaataatgaa gtgttatatg cagatgaaat
    17461 agaaaatata ttacctgcaa acataacagg aaaagatagt gttaactttg tggaaaatta
    17521 taaaaaaaac tgggcaatca atattttatt atacgaaaaa gctgaacaaa acctacgttc
    17581 aacagaagaa atagacttgt taaccgaaga ataccgaaaa caactaatta ttaatagata
    17641 tcagcaagag cttatttctc aaaaattaaa aaacataagc aatgatacat taataaacgt
    17701 gtatgaacgt gaaaaaacta atttcatttt agaagatcca attataaaag gggtatttgt
    17761 acagatacca agcaaagctc caaaccaaaa acaactaaaa aaatggttga cagaaatgaa
    17821 tgataatact ttagaaaaaa tcgaaaaata ctgtattcaa tatgcttctc ggtatatatt
    17881 ttttcgagac gactggactc catattcaca agttgataag ttatttcctc atcatgtaga
    17941 ctggaatgac ccaattttaa ctcgtggttt agttgagcaa aaagacagta tttcaacgta
    18001 ttttttgcgt attactggaa aggctaaccc cggtacaatt gaaccatttg aaatcgctct
    18061 acctaaaatc acaaatattc tctctcaagg agagaaaatg aattacatta aaaattttga
    18121 gaattctctt tacaataacg caattaaaaa acaaaaaatc accttcttcc tttcatccga
    18181 agaataatag ccactattat gttaaaaaaa atcacccttc tgtttgttgt atgtatattt
    18241 tcaataggag tattctctca aaattatatc gtagatcaag tcgtatggat tgtaggagat
    18301 gaagccatat tacaatcaga aatagaaacc gaaattctac gtttaaaata tgaaaaacaa
    18361 aaaacgccag aaagtccaga gtgtgttatt gctgagcaac tagccctaca aaaattattt
    18421 ttagcacaag ctaaaattga tagtattacg atctctgagg ctactataaa tcaacaggta
    18481 gaaagtcgga ttgcgtattt taccgaacaa ctcgggtcaa aagaaaaagt agaagcatat
    18541 tttaaaaaac cattatctaa aatgcgagaa gaactaaaag caatgctcca agatcagctg
    18601 atggtacaag aaatgcaaca aaaaattacg aaagatatca aaattacacc ttcagaagta
    18661 accgcatttt acgagaacat tcccaaagat agtttaccaa taataccaga acaagtagaa
    18721 attcagctta ttaccctatc tcctactata tctgttgcag aagttgagcg agtaaaaagc
    18781 aaattacgcg aattccgcga acgaattgaa acaggagata ttcagttttc taccttggct
    18841 attttatact ccgaagacag ggtgtcagcc ttacaagggg gcgaaattgg ttttcgttcg
    18901 cgttcaatgc tagtgcctga atacgcaaac actgcatttt ctcttactga ccctcaaaaa
    18961 gtttctcgta tcgtcgaaac agagtatggt tttcatatta ttcaacttat agcaaaacag
    19021 gatgatcaag tcaatagtag acacatacta atgcgtccaa aaatatcatt ggaagataaa
    19081 aatcttgctt cccaacgtct tgatagtatc gctcaagcaa tacgtacagg ctctataaat
    19141 ttcgaaaaag gagtggaaat atattctgat aaatcttcta caaaaaatag caacggatta
    19201 ctcataaata ctaaggatgg tagcactcgg tttcaatatg acgaattacc acaagaaatt
    19261 ataaatcaag tttcttttct aaacatcggg gagatttcta aaccatttag ctttaccaat
    19321 gaaaacggta aagaaatagt tgctatagta aaaatacatg ataaaattaa ggctcataaa
    19381 gcatctatta gtgatgacta tcaaacctta aaaatggtat acacgaatgt aaaaaacaca
    19441 caagcaatcc aacaatggat agaaagtaaa attgcaacaa catataatca gttgacccct
    19501 gcatatcgta cttgtaactt ttcatacccc cattggctaa aaaacagaga ataatcacaa
    19561 caaattgatg caaaatgcac atatccattg gcagattaac tacaagaata tgaaaaacaa
    19621 tgtctctatt atcgtgtttt tatatgttat gtgtattgca ttgtgctaca acgctaacgt
    19681 aactgctcaa aataaaacga ctgtttttct agagaatgct gaaacgctat cttttgatca
    19741 aaatgtccga aataacgctc aacttctaca aggaaatgtt cgatttagac atgataaaac
    19801 gttgatgctc tgcgacaacg catattttta tgaaaaaacc aattcattgg acgcttttgg
    19861 taatgtgaaa attatacaag gagatactct ttttatttat ggaaatactc tttattataa
    19921 cggcaataca cgtctagccc aaatgagaaa taacgtacga atgattaacc gcgatgccgt
    19981 gctaactaca caaaaactcg actacgacac atattcaaac attggctact acaccgattt
    20041 tggcacatta actgataaaa aaaatgtatt aacatcaaaa gaaggcgaat acttaaccaa
    20101 acaaaacata atcaaattta agaaaaatgt tgtgctaaaa aacgctgaat ttacactcta
    20161 ctcggacaca ttacactatc acacatataa ttcacttgct actattgttg gacccaccac
    20221 aatcatttct gatagtactc gtatttatgc agaaaaagga tggtataata cctctactga
    20281 agacacaaaa ttaactaaaa atggtaaaat tcagcataaa aacggaaaaa gcattcaagg
    20341 agactctatc ttttataaca aaaaatacgc aactatagaa gctttttcaa acaccttgct
    20401 aaaagattct gttcaacaaa tctccgtttt aggaaattat gggaaatatt ttgaacaaaa
    20461 cgaaaaagga tacgttacga aaaaagcaac ctttagagaa cattcctcag ctgatacatt
    20521 atttctaagt gccgattctt tgttatacga aagtattaag ggggatttct ccgttaaagc
    20581 tttccgaaat gtccgttttt ggcaaacaga ttttcaagga acatgtgatt ccctaaccta
    20641 caatcaaacc gactcgttac tcacaatgta ccacaatcca atactttgga acgaagaaaa
    20701 tcaaatctct ggaaaagtaa ttacattaaa aatgacagga gacaaagtgg atcatttcat
    20761 agtagaagaa gatgctttta ttctttctga agaaaacccc gaagaattca accaactatc
    20821 tggaagaaaa attataggtt acatactcaa cgataaaata agaaaaattg aaataattgg
    20881 gaatgcacaa agtatctatt acgttaaaga cggagaaagt gctatcggca ccaatagagc
    20941 agaaagtgct ctattaacta tctttctaaa tgattcaaat caagcagaaa aaatagttat
    21001 gtctcctcaa tctggaggca tcttataccc aacttttcaa aaaaatatat ccattattcg
    21061 gctcagtaac ttcttgaaac aaaataacaa tagaccaaaa aatcagtacg atatttatac
    21121 aaacagagag tagccattca actactctct gtttgtataa aatgaataat tattacttca
    21181 atccagctaa acttttttca atttcagcat cagaaacttc gtaattactc aaactaccag
    21241 acaaatattt atcgtatgca gccatatcta ttaacccatg tccagaaagg ttaaacaata
    21301 taaccctttc ttttccttct tcttttgctt taagagcttc tcgtatagtc atagctatag
    21361 catgtgttga ttctggtgct ggaacaatac cctctgcacg tgcaaataat acccctgctt
    21421 caaaagtctc attttgcttt attgcctctg cttcaattaa gccatctttg cgtaactgac
    21481 taaccaatgt acctgctcca tgataacgca atcctccggc atgaatttcg gctggctcaa
    21541 agttatgccc caatgtatac atcggtaata aaggcgtaaa cccttctgta tctccataat
    21601 catataaaaa tttaccacgt gttaattttg gacatgacgc aggttcaact gctataagcc
    21661 gaatatcttt cccctctgtt aacttatgac gtaaaaaagg aaacgcaata cccgaaaaat
    21721 tagaacctcc accaaaacat ccaatcacta catctggata atcacctgca atttccattt
    21781 gtttttctgc ctccaaacca ataattgttt gatgcaaagt tacatggttt aatacactac
    21841 ccaatgtata cttagtcttc tcatctttca tagctaattc catcgcctca gaaatagcca
    21901 tccccaaact accatttgtt ccaggaaact gttgttgcat acgtctgcca acctctgtta
    21961 aattactggg agaagcaact acatttgctc catacgtatt cattacagat ttgcgatatg
    22021 gtttttgttc ataactaatc tttaccataa acacttgcaa attaatccca aaatgttgac
    22081 aagcaacact catggcactg ccccattgac cagctccagt ttctgtggta atagaggtta
    22141 taccctcgat tttattataa taagcttgtg gtatagccga attcaattta tatgatccta
    22201 ctgggctaac actctcattc ttaaaataaa ttttcgctgg cgtatccaaa gctttctcca
    22261 aacctgatgc acgtactaat ggagttggac gatatatacg atataagtct cgtacttctt
    22321 cgggaatttc aatccatctt tcagatgaca cctcttgttt aatcaattct tttgcaaaaa
    22381 ttggttctaa atcagctgga gcaagtggct gttttgttac tggatgaaga ggaggcatag
    22441 gtttagtgtg catatcagcc acaatattat accactctct tggcatatca ttctcattca
    22501 atacaatctt ttttgttgtt ttcatacaaa catatgttta ttagtaaatt attaattcaa
    22561 atctctttaa ataggaaaag gtgacaaaat gtagaatcac catcgaaaag ctatcttctc
    22621 ataaaatagc ttgaaataaa ctagtataaa acaataatga agtatcatat aattttttaa
    22681 ataacagaaa aaaaaagaac cataggtgga tttcctacgg ttcttatcta ttttgtacaa
    22741 cacgcgacga agcatccacc ttatttataa gtcagattgc caccaccaat tgttgctgaa
    22801 tctttttttc atattatatt ttttgcaaag taaaagaata ttattgaatt agcaaaaaaa
    22861 tgtactacat ttttttgcta tgctctatat catatcccca tttaagcaat acacttcccc
    22921 aagaaaaacc agccccaaat gccgctaaca ccaaaatatc tccctttctt aaacgagact
    22981 cgtaatccca caaacataac ggaattgatg ccgctgacgt attcccataa tgttcaatat
    23041 taatcaaggt tttttctctt ggtaaactca ttcgtttggc tactgcttca ataatacgta
    23101 tatttgcttg gtgtggaact aaccaagcta tatcatcaga agtaagtgca ttctttatca
    23161 taatctcttc tgctacagct gacatatccg acactgctct tttaaataca gcttgccctt
    23221 cttgataaat aaaatgctcc ttattagtta ccgtctctat actagcagga tgtaaagatc
    23281 ctccagcttt taaatgtagg ttctccttcc ctaaaccatc tatacgcaaa atagaatcga
    23341 ctactccaac atcttcagta gttggctcta taagtacagc tgctgctcca tctccaaaca
    23401 aaggacacgt attcctatct tcataatttg tgatagcggt catcttttca gaacaaacca
    23461 atattatctt tttatacttc ccagattcta tcatagtggc acaagtatcc aagccaaaaa
    23521 taaaacctga acaagcagcc gccaaatcat acccataagc agaagtaatg cccaatgctc
    23581 cacaaattaa tgatgcatta gatgggaaaa caaaagatgg ggtagttgta caacagatta
    23641 acaaatcaac atcttttgtg tccaaactag tcttttctat taaatcttga acggctttaa
    23701 tccccatata agcagaacct ctacgttctc ctaatacatg gcgagtctta attccaatac
    23761 gagacattat ccactcatcg gaggtatcaa ccatcctact taattcctcg ttatctaata
    23821 tagcctcagg aacataaccc ccaattccat tgataacggc ttttattttt ttttccataa
    23881 tattctgtgc taaaaacaaa aactgcccta agtatttcgt cagggcttat ttagttgtat
    23941 tacaaagaaa gcataatcaa acgttatgca ttaacagttt tttctatcgc taatttgcct
    24001 ctataatatc cacactctcc acaaatagtg tgataaatat gcattgcacc acaattagag
    24061 catactgcca ttgtagctgg aaccgcttta tcatgcgttc ttctcttcgc cgttcttgtt
    24121 ttcgactgtt ttctttttgg atgtgccatc tctttttata taaattaatt actacttatt
    24181 attttatgtt ttttaacgct tcccatcgcg ggtctatttt ttcatttact acagttatat
    24241 ctgtactata cttttgtaaa ttcttttcca tctccttatt gcatccacca tcttgatgta
    24301 ttcgctgaag tggcaatatt aataccaaag actcatacaa gtaccatgct atattgaaca
    24361 caccttcttt ctctggcact atcaccaact cttcatcaac ttctgtatac gtttctccaa
    24421 acttaaccac caacactgaa tcttcatcta tttgtatttc cattacatcc agacatctat
    24481 cacacaaaac agatacaaat ccgtttagct taatagataa ttcaaacgaa tagttcagct
    24541 tttttacatc aattgacgcc ttaacattac ctcctctaat aatcccatct tctgcaacaa
    24601 aagcagaaaa aaaagactca tctaactcat aatcatacgt cgtattgtat gatacaatac
    24661 ttttgagagg aataatataa tgactcaaat ctgacactgt actagttttg ataaaagtgt
    24721 ggcaaagtta caaaatttta cgatactaaa acacaaatta cgtctaaaat atgttttttt
    24781 tctcttttta tttgtaaata gcttattatc aaaattaaac aaaagtctaa ttgcatcttc
    24841 cataatccat ccttattgga cataaaagat aattctaaag gtggttcctt tgtctatttc
    24901 tgtatgttga acaaatattt taccttgatg atattcctct ataatccttt ttgctaaaga
    24961 caaacccaac ccccatcctc tttttttagt tgtaaaacca ggattaaaga ttttacgact
    25021 attatgtttt gatatcccct tgccattatc tattacatct attctaattt ctttaccttt
    25081 ttgcgacaat tgaattttaa tgatgccatc accactcata gcatctgcgg cattcttgca
    25141 caaattttca atcacccact caaataatga tacattcaat tcacaataga gtacttcatt
    25201 ctcttgtata gaaagagtga tttttttgga gatgcgtgtc cgtaaataat ctacagcatt
    25261 gtgtataact tgatttaagt tcactatttc caaatctgtt ttggagccta tttgcgagaa
    25321 acgttctgca atggtacgta atctatttac atccttttcc atttcaccaa acatcgaatc
    25381 ttcaatgtat ttcgacttaa aaatttctgt ccatgccatt aaagaggata ttggagtccc
    25441 tagttgatgt gccgtttctt tagacaatcc cacccatact tcattttgtt ctgctttttt
    25501 ggtgcttaaa agagcccata taacaaatac taaaaatata aatactgcta ttaattgaac
    25561 aaaaggaaaa tacgacaatt gaataagcaa cagtgaattt ccatagtaca aatattgtcg
    25621 ttctgtatca ctaaatacaa tttcgatagg cgtattgctt tttttatatt cttcaactaa
    25681 ttgctgtaat tttctagatt cctgcaaacg ctctggaata tttttatacg aaataacact
    25741 gtcctcttgt gtaacaagta taactgggat agtagtattt gcctctatta cttgttgtac
    25801 aaatgaaata tcacaatact catccatatt aatcaactct ttcgtagcat ttgcccatat
    25861 ttgcatttta gacttttctt catctgaaag cttttgcacc aaactgttag ttacaacaaa
    25921 cgagcttatt gcaatcaata tcgcaagact tgaaaaaagc agtttaaagc gttgccttac
    25981 ttcgtaaatg gaagtcataa gaatgtatat tttgtgtacg ttaatgtaaa caaccaatac
    26041 atataacgta tattatataa aaaaagtata tccgtttatc tgtatttcgc atcttcagtc
    26101 atatcctgta aaatactcaa ataactctgg tatcgagatt gacttatacg aaattctcct
    26161 acagctttta taacagcaca atctggctct tgttggtgta aacaattatt atatttgcat
    26221 tgatgcgagt attcaaaaat atctctaaaa taatgtgcta cctcgtgctt ttttatatct
    26281 acaataccaa agcccttaat acccggagta tcaataatgt aacctcctga caatgtatac
    26341 atatgggaat tagttgttgt atgcattccc ttattatgca tttctgagat aagcccaatt
    26401 ttaacatcta atgtagggtc aatttggtgt atcaaacttg atttaccaac acccgaatta
    26461 ccagaaaaaa gagaaatatt tcctcttaaa cactctttca actcttcgat accctgcata
    26521 cttactactg aagtttctat acaacgatac ccaatatgtt gatacaactg ttttaaagca
    26581 tccaaataat tacgctcatt ttctatatac aaatcaacct tattaaacac aattatcaca
    26641 ggcacatggt acgcatcggc actcaccaaa aatctatcaa taaaacctgt agaagtttct
    26701 ggataattta ccgtaaccac taatatagct tggtcaatat ttgccccaat aatatgcgaa
    26761 tgtttcgaca aatttgatga ctttcgcaca atataattct ttcgatcgca tatatcataa
    26821 ataaatgaat gaccatttgt ttgtaaatca actagcacat tatccccaat ggctatagga
    26881 ttagtacttc taattccctt cagcctaaaa ttccccttta ttctacactc aacagatact
    26941 cctgtttgta gctgaacaag ataaatattt ccattatttt gaattacaag accttgcatt
    27001 cttatatttt aaaaagcttc tgtagctgta atataaaatt ccacattccc ataattagtt
    27061 ctggcaatgt ctgcccgtaa atgtacccgt gcatcattta acctaaatcg taaccctcca
    27121 ccatacgaca tttttaatcg aaaagaagga ttatcagttg acacaacatc cccaacagaa
    27181 taaaaaaggg ttgcctttaa tctattccat atcggtattc tatactctgt ttgtaattga
    27241 acaaacatat catctatgta ttttctctct ctaaatcccc gtaaattgtc agatcctcca
    27301 acagttggca acaattgaaa tgggatattc cctcccaaac gtgcatccaa atacaattgg
    27361 caagctatca attgaccaaa ccatgttggt aaaaaacacc taaaatcacc attaaatgaa
    27421 gctacatcaa aagtactacc taaaatcgaa aagtagttca atacacttgt ctttataaat
    27481 acacttgttt tctggggata aaatacatta tctcgcgtat cgtatgtaga aaaaacccct
    27541 aaacctgtca tccaataggg attccatcca gcactaccat aacgtgcgaa tatagcttct
    27601 atatgatttt caaaactctc acccacagtt atcttttctc ctctgagtga tacatatgga
    27661 ccaatttgcc aattatcatt tataaaatag ccagattgaa gcatcaactg aaagctctta
    27721 gatatataac tttcattata tattgaatta ctattaccta taccataata atacgtggga
    27781 taatagcgta aatttacatt cccgttaaaa aaccaattgc tatcgcctcc ataaaaacga
    27841 ggattagcat ttataataaa ttgatttcta aatgtgtata tcacactgcc agatatacta
    27901 ctaattttac gcaagtttgt actaggaaaa taatatccac caacgatacc caatgcaaca
    27961 ctcgtttctt gctgataata acccatagga atagtaaacc aactttcttt aataagcgga
    28021 caagagggca taacagtagc tgtatcacta tgcaatgaat cttttgcagc aactatctca
    28081 gatacttctg aaatagcata accattggta agcatcacat aaaaaaaaat aattacataa
    28141 ataacaaact tcattaaaga gcttctttag gtgttgattt acgttattct ggtataaaaa
    28201 accattcctc gttgttacaa agaatggtaa aatactcgtt tattttaaat attatattgt
    28261 cataatctct ttctctttag cgaatacagc atcatcaatc ttttttataa acttatcatg
    28321 aatcttttgt atctcagttt ctgcatcttt agccatatcc tctgctaatc catcttttac
    28381 agcttttttt atcgcatcaa tcacatctct acgaacatta cggacactaa tctttgcctc
    28441 ttcgccttct tgtttcgact gttttactaa cattttacgt ctttcctctg tcaaaggagg
    28501 aatgcctaaa cgaatcattt ctccattatt ttcaggagta atacctacat ctgaatgcaa
    28561 aattgctttc tcaatctctt ttattaaccc tttctcccat ggttgtattg caatagttct
    28621 tgcatcgggg gtagttaatg ttgcaacatt actcaatggt accattgaac cataataatc
    28681 gactcttaca gcgtctaaaa tacggacatt tgctttgcct gcacgtatat gtgccaaagc
    28741 atcatctaaa aacagaaggg tggtttccat cttctctatc gctgccgtta agtattgttt
    28801 tgtttccatc tttaagttat atttacgtcg ttatacatat actaatgttc caattttttc
    28861 tcctaaaatg accttcttta tattgcccgg agtgtttatt
//
LOCUS       DAOSRP010000006        28323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1848_length_28323_cov_5.309750, whole genome shotgun sequence.
ACCESSION   DAOSRP010000006 DAOSRP010000000
VERSION     DAOSRP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28323
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_28323_cov_5.309750"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLJ82_00830"
     CDS             <1..1091
                     /locus_tag="PLJ82_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOR40088.1"
                     /translation="KTIPVAKALCDRNKKEAFLAEIDIQHRETREKYASLKKVELISL
                     AEARNNALQIDWKKEQIHVPKITGLQIWKNYPIEELIPYIDWTYFFVAWQLKEKYPKI
                     LEDSPFKEEAIKLFADAQKILERMATEKLVVANGVFGIYPANTVEDTVEVYADENREK
                     PIALFHFLRQQTKKKNDEKNLSLADYIPSKKSGIGGYIGGFAITAGIHLTELTDSYEK
                     EGDDYSAIMCKILSDRLAEAFAEKLHADIRTTYWGYETIHATPQEMFEGKYQGIRPAI
                     GYPSIPDHSEKETLFNLLQATEHTGITLTENYAMFPNASVCGLYFDTARASYFNIGRI
                     GEDQVNDYANRKQIDITFLKKLIAKNLE"
     gene            1138..2091
                     /locus_tag="PLJ82_00835"
     CDS             1138..2091
                     /locus_tag="PLJ82_00835"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOR40089.1"
                     /translation="MNVADTLKQASSPLFTFELLPPLKGKGIDAIYKAIDPLLAFNPS
                     YINMTYHSAEVVYKTLADGKIEKRTVKKRPGSVALAAAIKYKYNIEVVPHVICSGFNQ
                     EETENILIDFHFLDIHNLLVLRGDPPKGMRVFQPKEDGHEHTLGLMSQINNMNNGRYL
                     DENLKNPEKTTFSMGVAGYPEKHMEAPNMVSDMKYLKQKVENGAEYIVSQMFFDTQKY
                     IDFVHQCRKEGILVPIVPGIKPIASMNDIKVLPQIFNIDLPEELVVELKKCTTNDEAR
                     QVGIEWCIKQSKELIAFGVPSLHYYTLGESDNIQKICKAVF"
     gene            2182..3804
                     /locus_tag="PLJ82_00840"
     CDS             2182..3804
                     /locus_tag="PLJ82_00840"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOR40090.1"
                     /translation="MTKSALQIARAAYQPKLPKALKGKVAIKEGAATQSVADQDAIKS
                     LFPNTYGMPIISFESSDKGIEVSKPISVGVILSGGQAPGGHNVISGIFDGVKKLHKDS
                     KVYGFLGGPGGLVDHKYIELTSEIIDEYRNTGGFDIIGSGRTKLEETAQYDKGVEICN
                     KLGIQALIVIGGDDSNTNACVLAEYYLQKNTGIQVIGCPKTIDGDLKNDMIETSFGFD
                     TACKVYSELIGNIMRDANSAKKYWHFIKLMGRSASHIGLECALQTQPNICIISEEVEA
                     KKQTLKEVVDSIAKVVATRAAKGNNFGIALIPEGLIEFIPEMKVLISELNDLLAHESD
                     VQKALNGLSKASAEVFCSLPENIAKQLTLDRDPHGNVQVSLIETEKLLIEMVGKRLKE
                     MKAEGQYTGKFAGLGHFFGYEGRCAAPSNFDADYCYSLGYNAAQLIATGKTGYMSSIR
                     NTTAPAEQWIAGGVPVTMMMNMERRHGAMKPVIQKALVRLDGAPFKTFAANRDKWATD
                     YCYVYPGPIQYFGPTEVCDQPTKTLQLEQAGK"
     gene            3959..4645
                     /locus_tag="PLJ82_00845"
     CDS             3959..4645
                     /locus_tag="PLJ82_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40091.1"
                     /translation="MKKLITTIFVTSIACWGLFAQGTNFARSEGNVTVDVNFSPGVNA
                     PLALNLIQGRYFLNETTAIRTGINLTIVSNTDKETTPGAGNGGSVLTKDWLNRNIAFT
                     LNPGIEKQYPITDRLSTYIGGDILFTYKTDYALYENSNSIKTETKNATGSTTYGAQFV
                     AGFDIAIFKGLYLGAELNYGATWTTFADQVVINTAEGVETTTTTENNNVNFNLTPNII
                     PSIRLGWTFK"
     gene            4775..4850
                     /locus_tag="PLJ82_00850"
     tRNA            4775..4850
                     /locus_tag="PLJ82_00850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4811..4813,aa:Pro,seq:ggg)
     gene            complement(4960..6183)
                     /locus_tag="PLJ82_00855"
     CDS             complement(4960..6183)
                     /locus_tag="PLJ82_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR40092.1"
                     /translation="MKVRNWTKWTPLFLSNFMGVLNDNFLKYSIIFMAISWSLPTWFT
                     QSQLIALVSFALVIPYVFLSPLGGMLATRYSKRKVFLFFKLLEIPIMAIACVAFYYQY
                     VFLAIFSVLLMGIQSSLYSPAKYSLIRDIGGEKGVSFGTGVFEMMAFLGILAGTVLAS
                     YMADRYSYVWVLICFLGFAIIGYLVTSQIRAKEIAAPQELKIALHPFAFIIQSYHKSR
                     AFPMLNAAVFGSAFFWFLGSILQMNIVIHSTVVYGLSNTETGVIVSIVAIALGVGCWI
                     AGKILTNTNIYVLLLVGISGIAISSFVLGIYCYPLRTFLALLVILSLCGGLFQVTCLS
                     LIQKTAKEISLADIFAYVNLLTFICILIGAGLFSLINWMSGDNSFYIFFLLSAISFLL
                     TFFYFFKVMSIRKLP"
     gene            complement(6258..7019)
                     /locus_tag="PLJ82_00860"
     CDS             complement(6258..7019)
                     /locus_tag="PLJ82_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40093.1"
                     /translation="MLNGSLDLFFVLFSYLFPLIMTVMFIFLIAIIPQWRSDKQKKAI
                     LIFLIAWLPWLLALVVWEVFVADPPYPVFDFFFSLSGLITFPFIYNYIVVVTSQRGLT
                     FKKWIAWLSLPFLLLISWIIIVLIQGELPFVYSVSDFIAQLHSAESWIRVAGFLGYCV
                     YAILIVILGIKRYLMYKENIANDFSYTHSISLNWIIYMLGLFFIFAALSGISFFAGDV
                     FVHATVNIINTVLILMLVCCVILMILYFKQSCIRN"
     gene            complement(7169..7612)
                     /gene="rplI"
                     /locus_tag="PLJ82_00865"
     CDS             complement(7169..7612)
                     /gene="rplI"
                     /locus_tag="PLJ82_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR40094.1"
                     /translation="MEIILKENVINLGFKDDIVTVKDGYGRNFLIPTGKAVLATSSAK
                     KVLAENLKQRAHKLAQLKQQADEIAAQLEGLTLTIGVKASSTGKIFGSVTTIQVAEAL
                     EAKGIVVDKRIIVLKDAIKEVGTYKATLKLHKEVTSEISLEVIAE"
     gene            complement(7636..7905)
                     /gene="rpsR"
                     /locus_tag="PLJ82_00870"
     CDS             complement(7636..7905)
                     /gene="rpsR"
                     /locus_tag="PLJ82_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOR40095.1"
                     /translation="MAQNSSEIRYLTPPSVDNKKKKYCRFKKSGIRYIDYKDPEFLKK
                     FLNEQGKILPRRLTGTSLKFQRQVAQAVKRARHLALLPYVTDMMK"
     gene            complement(7909..8259)
                     /gene="rpsF"
                     /locus_tag="PLJ82_00875"
     CDS             complement(7909..8259)
                     /gene="rpsF"
                     /locus_tag="PLJ82_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR40096.1"
                     /translation="MNHYEAVFILTPVLSDVQMKEAVEKFKSLLEQEGAEIVNVENWG
                     LRKLAYPIQKKSTGFYNLIEFNAESPIVDKLEIQFRRDERVIRFLTLKMEKYAVEYAE
                     KRRKLKSTKEVKEN"
     gene            8378..8845
                     /gene="bcp"
                     /locus_tag="PLJ82_00880"
     CDS             8378..8845
                     /gene="bcp"
                     /locus_tag="PLJ82_00880"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOR40097.1"
                     /translation="MAVKVGNKAPNTLGKDENGNLIQLSDYQGKKVILYFYPKDNTSG
                     CTAQACSLRDNYSDLKNLGYVVIGVSIDSETSHQKFIQKFDLPFPLIADTDKTLVETF
                     GVWAEKSMYGKKYMGTVRTTFIIDEQGIITHIIEGKAVNTKEHATQLIQLIQS"
     gene            8868..12554
                     /gene="purL"
                     /locus_tag="PLJ82_00885"
                     /gene_synonym="purI"
     CDS             8868..12554
                     /gene="purL"
                     /locus_tag="PLJ82_00885"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOR40098.1"
                     /translation="MIQFFETPEKNILAVQYTKELSKEDNNKLVWLFGNANILDNSSI
                     TGNFVGPRREMITPWSTNAVEITQNMGIEGIVRIEEFYPADTTGDFDPMLQRKYDKLT
                     QEIFDVIKQPEPIVFIDDIAAYNQQEGLALNDDEINYLNNISQTIGRKLTDSEVFGFS
                     QVNSEHCRHKIFNGKFVIDGEEKELSLFKLIKKTSEYNPNKLVSAYKDNVAFNEGPVI
                     EQFAPKSGDKPDSFTTKEIKSVLSLKAETHNFPTTVEPFNGAATGTGGEIRDRLGGGK
                     ASLPIAGTAVYMTSYPRIESNRSWEENIKERNWLYQTPEQILIKASNGASDFGNKFGQ
                     PLICGSVLTFEHEENNKKYGFDKVIMLAGGVGFGTMRDALKGEAKVGDKVVVMGGDNY
                     RIGMGGGAVSSVDTGQYDNAIELNAVQRANPEMQKRAANVIRTLAESDRNPIVSIHDH
                     GAGGHLNCLSELVESSGGKIDMSKLPIGDSTLSAKEIIGNESQERMGMLMDEKDIDLV
                     QKIADRERAPMYVVGETTGDMQFVFEQANGEKPIDLRLDYMIGNPPRMVITDNTLNET
                     FEEVSVSDSKIEEYITNVLRIEAVACKDWLTNKVDRSVTGKVATQQCAGELQLPLNNL
                     GAVALDYQGKAGIATSIGHAPLAALVNPEAGSVLAIAEALTNLVWAPLTEGLTSISLS
                     ANWMWPCKNSGEDARLYKAVEACSDFACSLGINIPTGKDSMSMTQKYGNDKVFSPGTV
                     IISAAAEVSDVTKTISPVIINDTKTAVYYVDFSFDALKLGGSVLYQTLNKLGNDTPTV
                     QDSEYFATAFTAVQELIANKLVLAGHDISEGGMITALLEMCFANTKGGLSVNLSELKD
                     FSLTTVLFAENPGVLLQVKNTVEVEKLLTENGIGFAKIATPINDRKLILTKDSTHQEL
                     SIDTLRDVWYKTSYLLDRKQSGEVCALQRFENYKNSPLQFAFGTEFTGKLAKYGISND
                     SRGKSGAKAAIIREKGTNGEREMAYALYLAGFDVRDVHMTDLANGRETLEDIQMIVFC
                     GGFSNSDVLGSAKGWAGGFLYNEKAKTALENFYKRPNTLSLGICNGCQLMIELNLINP
                     EHTKRSRMLHNDSHKFESNFVGLHIPENNSVLFNSLSGSKLGIWVAHGEGKFSLPESE
                     DKYHVIAKYGYNTYPANPNGSDYNVAGICSADGRHLAMMPHLERAIFPWQWAYYPIDR
                     KTTDEVSPWIEAFVNAKNWILAHK"
     gene            12578..13159
                     /locus_tag="PLJ82_00890"
     CDS             12578..13159
                     /locus_tag="PLJ82_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR40099.1"
                     /translation="MILSFVYSRIYEMNAWKLFSTFFKIGTFTFGGGYAMLPLIEQEV
                     VHKQQWLNTEEYTEMLSLVQTAPGPISINSAVFIGYKLHRWKGALSCLLGVILPSFII
                     ILLIAVAFTEINSNPIVERIFKGIRPAVVALIAVPVIRMIQREKLHWTSGLIALLSII
                     LVWLLGYSPVWVVLGAIIYGIVKEYLTTKTKKP"
     gene            13156..13677
                     /locus_tag="PLJ82_00895"
     CDS             13156..13677
                     /locus_tag="PLJ82_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR40100.1"
                     /translation="MIYLELFWSFFTIGLFGFGGGYSILSLIQHEVVDIQGWLTIADF
                     TNIVAISQVTPGPIAINCATYVGYTATNSILGSAVATIAVSLPSLFIMVLISRFINAF
                     RSNPYVAAALNTLKPAIIGLIASAALLLSNPSNFIDYISFIIFGTVFVAAYCKVNPIF
                     LILLAGMAGLVIY"
     gene            complement(13681..14985)
                     /locus_tag="PLJ82_00900"
     CDS             complement(13681..14985)
                     /locus_tag="PLJ82_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR40101.1"
                     /translation="MRTPLLVIWLLFVSSILFGQQKWTLEQCLEHAKVENIQVQQRLI
                     QAENAKINLQQSKQNLLPDLNMYVGQSFSFGRSIDETNTYQNNNVQNTNFSLSSSLNL
                     YDGGRMFYAIEKSKLDFQALLLDVEQEKSTIELQVLLHYFQVSYAKKQVELAENQYAL
                     TSNQLEKTKALIDAGRLPEGEIYQVKAQLSQEELAILQAKHEVNNLLMQFAQLLLIDD
                     VDSFDIEDVEIVAREEYLLPMFENVYTSTIATMPSIRAEQLRLESANQQVRIEKSAYY
                     PRLSMGASYGNGYYNAENRLNLPFSEQWQQNQNIAVTLNLQIPIFNKMSVINRVKQSE
                     LAVKSQELVAKNTKIQLRQELQQAYLLTVAAKEKISVSENNVRAMQKSFDYQTEKFDS
                     GVSTIYEYNEAKNALTKALSNQTQAMFEFLFRSKAIELYMGK"
     gene            complement(14990..16072)
                     /locus_tag="PLJ82_00905"
     CDS             complement(14990..16072)
                     /locus_tag="PLJ82_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR40102.1"
                     /translation="MKKFLKISAIVILVVLVLGTFYMLWKKSQPEKTSFSTTSPIVTT
                     IENKTVATGKVEPRTVVQIKPQISGIITEIYKEAGQQVKAGDVIAKVQIIPDMVSLSA
                     AESRIKRAQIALEKAQIDYDRQKSLFDKGVISQEAYQTATIELKSSQVDLVDAQNNYD
                     LMKEGITRNASNYSNTLIRSTINGMILDIPQKVGSSVIQSNNFNDGTTIATVANMKDM
                     IFVGKIDETEVGKIKVGMPIVLVIGALEGKTFDATLEYIAPQGISDGGAIVFEIKAAV
                     SLSDDVFVRSGYSSNAQIILEKKENILALPESALIFEKDSVFVEVQTKDTLVFDKRAV
                     KLGLSDGINIEVLEGVTQNEKIKLVK"
     gene            complement(16100..17356)
                     /locus_tag="PLJ82_00910"
     CDS             complement(16100..17356)
                     /locus_tag="PLJ82_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR40103.1"
                     /translation="MRIVDIDRWQEIWHTIKQNKLRSVMTAFGVFWGILMLVIMLGSG
                     KALENGILDGTENFERNSCFMFAGTTTEPYKGFQRGRSWYITSSDLVAIEKNVEGVKF
                     TVPYVFPTNTTENNVMNGMKVGSFSVQGITPELYLVNPQILLQGHLINKLDELGKRKI
                     CVIGEKVKEQLFLADETVVGTYIRVNGVCFQVVGVIKSATQINIGSQPENTVFVPYTT
                     AKQVFNLGDRISYISVVAEDWADISKVEEDTKEVLKARNSIAPSDNEAIWSFNMKQQF
                     QQIGALLLGIQLLIWIVGIGTLLAGIIGVSNIMLITIRERTKEIGIRRAIGATPKVII
                     GQILVESLVLTVLAGMGGLVLGVWILHFANGLIPADGMFFKNPHIDFGVAIIATGIMS
                     LFGVLAGIIPARRAIRIKPIDAIREE"
     gene            complement(17361..18626)
                     /locus_tag="PLJ82_00915"
     CDS             complement(17361..18626)
                     /locus_tag="PLJ82_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR40104.1"
                     /translation="MIHFQLWEEIFASLRRNKLKTALTGFAVAWGIFMLIVLLAAGNG
                     LKNGMALNYKDDSKLAVWMWSGRTNLAYKGLPTKRNIQFTNTNETILRKDFDASIGEL
                     SPLIDMRGKNVMYKNEKGIHDVRGVLPEFVPIEALEMLPNQGRFVNQLDCDHKRKVVV
                     VSSKFVQDFFKDESPLGKQLSIDGIMFTVIGVFKEVPRSWQKLVYIPLPTAQSLYGFG
                     DKIHEISFNLHMQNETNVDESKQIMDNIKQRFASYHQFDVEDEGAMGYWNTLESFLFT
                     QKLFAGISIFVWIIGVGTLIAGIVGISNIMLISVKERTKEFGIRRALGAKPSSIIVLV
                     VLESIIITAFFGYIGMVFGVGLTEGVNYVMKQMPMDETGAVFFYNPTIQLDLVMVATV
                     LLIIAGVIAGYFPARKAIRIKPIDALRDE"
     gene            complement(18630..19304)
                     /locus_tag="PLJ82_00920"
     CDS             complement(18630..19304)
                     /locus_tag="PLJ82_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR40105.1"
                     /translation="MIRLTNIHKTYYNGSPLKVLKGVDLHIKEGEFVSIMGASGSGKS
                     TLLNILGILDNYDEGDYYLDNQLIKNLTETQAAQYRNELIGFVFQSFNLIHFKNALEN
                     VALPLYYKGIARKKRNAIALEYLEKMGLKDWAHHYPNELSGGQKQRVAIARALISNPR
                     VILADEPTGALDSQTSVEVIDLLKAINQQGVTVIVVTHENEVAASTERIIRMKDGIIQ
                     PQDIHQ"
     gene            complement(19404..20756)
                     /locus_tag="PLJ82_00925"
     CDS             complement(19404..20756)
                     /locus_tag="PLJ82_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR40106.1"
                     /translation="MNYHRIFRTYFLYKLIFKYVRCIHAYILHRSYTVYNAENIPTGK
                     PFIMVANHQHGILDALALLFMIKDGRYPVFMARADVFKNKYVAQLLVFFRLLPAFRMQ
                     DVGKNRVLENEKVFATTAQLLAQHNGILALFPEGGHQEREQLGKLKKGFARIAFQTAS
                     CANFETEIFVLPVGIHHIGGLFQSKVSVCVGKPISVLTFQELYMQEPEKAQQQLLEQT
                     ARAMSSNMLDIKETTYYNEYLVLCTIARIYRKNNAAIQERYATDLAIEQAVVSRVELL
                     KVHQETTYVELMKLTKNMDELIVSLHLSYELVCEKKHSIAKRGMHVFITILLIPIQIY
                     AFVHQIIPFLLTSVCSRNAGDELLKVSLQLALGALFIFPLWNGFLVFILFLLEVPFSW
                     LVCYGISLLFSIWVFFRSVSAWSFCQAWCRRQLVRLTNRSAIKEIEGILKNIFSIIGV
                     "
     gene            20857..21381
                     /locus_tag="PLJ82_00930"
     CDS             20857..21381
                     /locus_tag="PLJ82_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR40107.1"
                     /translation="MQQTEWFPIVTETGERIGKATRKECHSGSFLLHPVVHLHVFNAK
                     KQLFLQQRAANKDIQPNKWDTAVGGHVDDGESIQIALVREAYEELKIKDFSANFLFQY
                     PFRSQVEHELVHSFWVQYDFPIQANLEEIQDARFWVIDDIKNSLGKQLFTPNFEDEFT
                     KLLSHPDIIQFLHP"
     gene            complement(21447..21929)
                     /locus_tag="PLJ82_00935"
     CDS             complement(21447..21929)
                     /locus_tag="PLJ82_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR40108.1"
                     /translation="MGKFNKNSSSRELPTISTASLPDIIFMLLFFFMATTQLKEVDFK
                     VSIKEPKATELEKLDKKNLVRFIYVGTPTDKYRGVYGSETRVQLDDAFADVSQIEDYI
                     VAQRAAMNEYEQSLLTVALRVDEDAKMGIVTDIKQSLRRAYALKINYVSRQRDNSQGF
                     "
     gene            complement(21929..22519)
                     /locus_tag="PLJ82_00940"
     CDS             complement(21929..22519)
                     /locus_tag="PLJ82_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR40109.1"
                     /translation="MSRNKRKVPEINSSSMADISFLLLIFFLVTTTMVVNKGLARRLP
                     PPIPPDQKTEDLKVKERNVLVVLLNSDNQLMVQREYISVTELRKKAKEFIVNANNAQD
                     MPEKKAEEVQFFGEVPLTRDHVISLQNDRTTSYKAYIEVQNELIAAYNELREELAKSK
                     FGVSYAELDDDRKTAIQYIYPQKISEAEPKNYGGNK"
     gene            complement(22557..23009)
                     /locus_tag="PLJ82_00945"
     CDS             complement(22557..23009)
                     /locus_tag="PLJ82_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40110.1"
                     /translation="MLNNLSKITLYVLIGITVVVSVLFFWGGAVDEYAEYIEPNYTQA
                     MMILMYVLFGIAILITLVSLLIAFLENFKVDKKTATKPLISIFALLALVGFAWLIADG
                     SPMQILGLDAQPTENEIKFVDVQIKAIYLLSGVAVLSMFLSFFTKRLK"
     gene            complement(23037..23840)
                     /locus_tag="PLJ82_00950"
     CDS             complement(23037..23840)
                     /locus_tag="PLJ82_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR40111.1"
                     /translation="MKKLFAVLAVIFSLTFGLTFSALAQDAAPAVDSTSVEQVATDEA
                     VVEETVAPVVEEGGIHQALKQKFIEGGAGFMASIALCLILGLAVCIERIIYLSLATSN
                     SKKLLEEVEAALAQGGVEAAKEICRNTRGPVASIFYQGLTRIDEGIDVVEKSIISYGS
                     VQTTLLEKNLSWIGLFIGLAPMLGFLGTVIGMIEAFDRIQQAGDISATIVAGGIKVAL
                     ITTVGGLIVAIILQVFYNFIVSRIESVVSDMEDSSISMLDILVEQSNKK"
     gene            complement(24731..25033)
                     /locus_tag="PLJ82_00955"
     CDS             complement(24731..25033)
                     /locus_tag="PLJ82_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40112.1"
                     /translation="MTDELIKYHALEFFRQHSRYLSEDSIFDIERDLRNYQVENRKLY
                     EFKYQDAIEIGILKTYLLDNDIIIFKESARVTGERLYGLSEYGKELLKRNKEHSYQ"
     gene            complement(25106..25243)
                     /locus_tag="PLJ82_00960"
     CDS             complement(25106..25243)
                     /locus_tag="PLJ82_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40113.1"
                     /translation="METRITLHTPRVEQQHYAHPKHGSQKSLVYFESSLKPITFEQKL
                     N"
     gene            complement(25377..25461)
                     /locus_tag="PLJ82_00965"
     tRNA            complement(25377..25461)
                     /locus_tag="PLJ82_00965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25425..25427),aa:Ser,seq:tga)
     gene            25608..28049
                     /locus_tag="PLJ82_00970"
     CDS             25608..28049
                     /locus_tag="PLJ82_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOR40114.1"
                     /translation="MIYPAHFETKIGFDSIRAILKHHCISPLGEEKVDEMTFSDSLVT
                     IDTRLSQTNELLTLLNEHDSFPTDAYVDIRPALSRIRIEGTYLLENELFDLYRSLLTI
                     QHIVQFIQQKDAKRFEQLHLLAKDVLLFPPITKHIDTILSKFGKIKDSASPELAFIRR
                     SKSQLSGTISRLLQQIIRKGQTDGYIDNEVTPAVREGRLVIPVSPSYKRKINGIIHDE
                     SASGKTIFIEPAEVVETNNQIRELELQEKREITRILVQCANFIRPMLPDLLHSYHFLG
                     TIDFIRSKALLAIQLQATKPVIENNPHIDWQDTFHPLLYLSLRKQGKAIVPLSIKLND
                     QERILVISGPNAGGKSVCLKTVGLIQYMMQCGLLVPMKESSTMGIFQRIFIDIGDEQS
                     IENDLSTYSSHLTNMKFFAKHCQEATLLLIDEFGTGTEPLIGGAIAEATLHVFNQQKA
                     FGVITTHYTNLKHFATENEGVVNGAMLYDRHLMQPLFQLEIGNPGSSFAIEIARKIGL
                     PEHIIADATLKVGAEHIDFDKNLQDIARDKRYWENKRQQIRLKEKKLEETTEQLRKEL
                     EQVTKQRKQVLKDAKNEASLLLQQANASIENTIRTIKQSQADKEKTAIARKKLEEYKQ
                     SINDTQQQDVLEQRLSAIKPLKANKEKSEQTENKTLHVGDAVRIIDKETTGTIVSIHQ
                     KTAIIAIGQLKSTIKLDQLERISNKQQKQLQRHISYISQRTQDEMRERKLQFKQEIDV
                     RGKRAEEALQIIRYYIDDAFIVNASRVRILHGTGTGALREVIRQYLSSIPGITRISDE
                     HIQLGGSGITVVDFE"
     gene            complement(28137..>28323)
                     /locus_tag="PLJ82_00975"
     CDS             complement(28137..>28323)
                     /locus_tag="PLJ82_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40115.1"
                     /translation="TGIQSTFDAAVVAANGTISADADFSIINLAGVDVTAQNGSLAAG
                     AYIVKLANGAAQVVTLK"
BASE COUNT         9628 a         5454 c         4658 g         8583 t
ORIGIN      
        1 caaaaaccat tccagttgct aaagcattgt gcgatagaaa caaaaaagag gctttcttag
       61 ctgaaattga tatacaacac cgagaaacca gagagaaata tgcctcgttg aaaaaagtag
      121 aacttatttc attggctgaa gcacgtaaca atgccctaca aatagattgg aaaaaagaac
      181 agattcatgt tcccaaaata acaggcttgc aaatttggaa aaattaccca attgaagagc
      241 ttattccata tatagattgg acatactttt ttgtggcatg gcagttaaaa gaaaaatacc
      301 cgaaaatact agaagatagc ccttttaaag aagaagctat taagctattt gctgatgccc
      361 aaaagatact tgagcgtatg gcaactgaaa aattggtagt cgccaatgga gtatttggca
      421 tttatccagc caatacagta gaggatacag tagaagtata tgctgatgag aatcgcgaaa
      481 aaccaatcgc attgtttcat ttcttgcgtc aacaaaccaa gaagaagaac gacgaaaaaa
      541 acctttcgct tgccgattat attccttcta agaaatcagg gataggcggt tatattggcg
      601 gttttgctat tactgcgggc atacatctta cagaattaac agatagttac gaaaaagaag
      661 gcgacgacta ttctgccatt atgtgcaaaa tattgagcga tagattagcc gaagcatttg
      721 ccgaaaaatt acatgctgat atacgtacaa cctactgggg ttacgaaaca atacacgcaa
      781 caccacaaga aatgtttgaa ggcaagtacc aaggcattcg acccgccatc ggctacccat
      841 caatccccga ccactctgaa aaagaaacct tattcaactt gctacaagca actgaacata
      901 caggcattac cttgacagag aattacgcca tgtttccgaa tgcaagtgtt tgcggtttgt
      961 attttgatac tgccagagca tcgtacttca atataggacg aattggagaa gaccaagtaa
     1021 acgattacgc caatcggaaa caaattgata tcactttttt aaaaaaactc atagcaaaaa
     1081 atttagaata aaataagttt tcgtaatttt gttggcttaa cgaaaaaaac agataacatg
     1141 aacgtagcag atacactcaa acaagcaagc tctccccttt ttacgtttga actattgcct
     1201 ccattaaaag gaaaaggcat tgatgccatt tacaaagcaa tagaccctct tttagcattc
     1261 aatccttcgt atatcaatat gacctaccac agtgccgaag tagtatataa aaccttggcg
     1321 gatggtaaaa ttgaaaaaag aaccgtaaaa aaacgccctg gaagcgtagc attagctgct
     1381 gctatcaaat ataaatacaa tattgaggta gtgcctcacg ttatttgtag cggttttaat
     1441 caagaagaaa cagaaaatat attgattgat tttcattttt tggatattca taatttattg
     1501 gtacttagag gcgacccacc aaaaggaatg cgtgtatttc aaccaaaaga agacggacac
     1561 gagcatacct tagggttgat gtcgcaaatt aacaacatga ataatggacg gtatttggat
     1621 gaaaatttaa aaaatcctga aaaaaccact ttctcgatgg gagtagcagg atatcctgaa
     1681 aaacacatgg aagctcccaa tatggtatct gacatgaaat acttaaaaca aaaagtagag
     1741 aatggtgctg aatatattgt gtcgcaaatg ttttttgata cacaaaaata cattgatttt
     1801 gtacatcaat gtcgcaaaga aggtatcctc gtgccgatag taccaggcat taaacccatt
     1861 gctagcatga acgatatcaa agtattacca caaattttta atattgactt acccgaagaa
     1921 ttagtggtgg aattaaaaaa atgcacaaca aacgatgaag cccgacaagt aggtatcgaa
     1981 tggtgcataa aacaatcaaa agaacttatt gcctttggtg tgcccagttt gcattactat
     2041 accttaggag aatcggacaa tatacaaaaa atatgcaaag cggtatttta acaacaataa
     2101 tataaataga tagtatatat ttttttcttt ttattgtatc tttgcaagcg aaaaaactta
     2161 aaccacaaaa cacataacat tatgactaaa agtgcattac aaatcgcaag agctgcctat
     2221 cagccaaaat tacctaaagc cttaaaaggg aaagtagcta ttaaagaagg agctgctacc
     2281 caatcagtag ccgaccaaga tgccatcaaa tcattgtttc caaacacata tggcatgccc
     2341 attattagtt tcgaatcatc agacaaaggt attgaagtaa gcaaacctat ttcagtaggt
     2401 gttattttat ctggaggtca agctcctggt ggacacaatg taatttctgg tatttttgat
     2461 ggcgtaaaaa aattgcataa agactctaaa gtgtatggat ttttgggtgg acctggtggt
     2521 ttagttgacc ataaatacat tgaacttaca agcgagatta ttgatgaata ccgcaatact
     2581 ggaggttttg atattattgg ttctggtcgc acaaaattag aagaaacagc acaatacgat
     2641 aaaggcgtag aaatttgcaa caaattaggc attcaagcac ttattgttat tggtggagat
     2701 gattcaaaca caaatgcgtg tgtattggca gaatattatt tgcaaaaaaa tactggtatt
     2761 caagtaattg gttgcccaaa aaccattgat ggtgacctta aaaatgatat gatagaaaca
     2821 tcatttggat ttgatactgc ttgtaaagtt tattcagaac ttatcggcaa tatcatgcgt
     2881 gatgcgaatt ctgcaaaaaa atactggcat tttattaaat taatgggacg ttcagcttcg
     2941 catatcggac ttgaatgtgc tttacagaca caaccaaaca tttgcatcat ttcggaagaa
     3001 gtagaagcaa aaaaacaaac gctaaaagaa gtggttgata gcattgctaa ggtggtagct
     3061 actcgtgctg cgaaaggtaa taattttggt attgcactta ttcctgaagg gttaatagag
     3121 tttattcctg aaatgaaagt tttaatttcg gaattaaacg acttgctagc acacgaatcg
     3181 gatgttcaaa aagcactcaa cggattaagc aaagcaagtg ctgaagtatt ctgtagctta
     3241 ccagaaaata ttgctaagca attaaccctt gatcgcgacc cacatggcaa cgtgcaagta
     3301 tcgttgattg aaacagaaaa attgttgatt gaaatggtag gtaaacgatt gaaagagatg
     3361 aaagccgaag gacaatacac aggtaaattt gctggcttag gacatttctt tggttacgaa
     3421 ggacgttgtg cagctccatc aaactttgat gctgactact gctattcatt aggatacaat
     3481 gcagctcaat tgattgcaac aggcaaaaca ggctatatgt cgtctatccg caatactact
     3541 gcccctgcag aacaatggat tgctggtggt gtacctgtta ctatgatgat gaatatggaa
     3601 cgtcgtcatg gtgcaatgaa acctgtaatt caaaaagcat tggttcgttt agacggtgct
     3661 ccatttaaaa catttgcagc caatcgcgac aaatgggcta ctgactattg ctatgtatat
     3721 cctggtccaa tacaatactt tggtccaaca gaagtatgcg accaacctac aaaaaccttg
     3781 caattggaac aagcaggtaa ataacatatc tttttttact caaataagcc tcttaaaaaa
     3841 caagaggctt attttgttaa tatatgttaa ttaaaatgtg cgtttgtaaa ctgtaaagta
     3901 tatttgtgta gttttgttta cacttaattt tatattattc attaaaaact aatttattat
     3961 gaaaaaatta attacaacaa tttttgttac aagtattgct tgttggggtc tatttgcaca
     4021 aggaacaaat tttgctcgtt cggaaggaaa cgtaacagtg gatgttaact ttagtccagg
     4081 tgttaatgct ccattagccc taaacttaat tcaaggacgt tatttcttaa atgaaactac
     4141 ggctattcgt acaggaataa accttacaat agtctctaat acagataaag aaactacgcc
     4201 tggtgctggc aatggtggct ctgtattgac taaagattgg cttaatcgga acattgcatt
     4261 taccttaaat cctggtattg agaaacaata tccgattaca gacagattaa gtacatatat
     4321 tgggggagat attctcttta cgtacaaaac cgattatgcg ttatacgaaa atagcaattc
     4381 aataaagaca gaaacaaaaa atgcaacagg aagtactacg tatggagcac aatttgttgc
     4441 tggttttgat attgccattt ttaaaggatt gtatttggga gcagagctta attatggtgc
     4501 tacatggact acatttgcgg atcaagtagt gataaacacg gctgagggcg tagaaactac
     4561 aactactacc gaaaacaaca atgttaactt caacttaaca ccaaacatta taccttctat
     4621 tcgtttaggt tggactttta aataattctt tttacttact tggtaaataa aaagcagcta
     4681 ttttagctgc ttttttcgta taataatagc gaataaatta gatttttgat ttgacaaaca
     4741 caaaaaatta cgtacatttg cattcccaat agttcggggt gtagctcagc ccggtttaga
     4801 gtacacgtct ggggggcgtg tggtcgcaag ttcgaatctt gtcaccccga ctaacaacaa
     4861 agcaagactt tccacaagaa agtcttgctt tgttgtatag ggacatgatg cttccacaac
     4921 aaacaactat ttcaggtctt tgctttttat tctatttatt caaggcaact ttcgtataga
     4981 cattacctta aaaaaataaa aaaacgtcaa caaaaaagat atcgcagata aaagaaaaaa
     5041 tatataaaaa ctattatcac ctgacatcca atttattaga gaaaataatc cagcaccaat
     5101 taagatacat ataaaagtaa gcaaattaac ataagcaaaa atatccgcta acgaaatttc
     5161 cttagctgtt ttttgaataa gagacaaaca cgtaacttga aataatccac cacacaggga
     5221 aagaatgacg agtaatgcta aaaatgtccg taatggatag caatatatac ctaaaacaaa
     5281 gctactaatt gctattccac taatacctac caataatagt acatatatat tggtgttcgt
     5341 taaaatttta ccagctatcc aacaccccac tcccaatgct atggctacta tggacacgat
     5401 gacacccgtt tctgtattag ataatccgta tacaacagta ctatggatga cgatattcat
     5461 ctgcaatata ctacctaaaa accagaaaaa agccgatcca aacacggcag catttaacat
     5521 gggaaaagct cgagatttat gatacgattg aataataaat gcaaatggat gtaatgctat
     5581 tttaagctct tgaggagcag caatttcttt tgctcttatt tgagatgtaa ctagatagcc
     5641 aataatagca aaacctaaga aacatatcaa tacccagaca taactatacc tatcagccat
     5701 gtaagatgcc aatactgtac cagctaatat tccaagaaat gccatcattt cgaatacacc
     5761 cgttccaaac gatacccctt tttctccccc aatatcacgt attaaactat atttggcagg
     5821 agaatacaag ctactttgaa tccccattaa caaaacggaa aaaatagcta aaaacacata
     5881 ttgataataa aacgccacac aagctattgc cataatgggg atttctagca acttaaaaaa
     5941 aagaaaaacc ttgcgtttgg aatagcgagt tgccaacata ccgcctagag gagataaaaa
     6001 aacatagggt attactaacg cgaatgatac cagggctatt aactgagatt gagtgaacca
     6061 agtaggcaat gaccaggata tcgccataaa aataatggag tatttcagaa aattatcatt
     6121 taatacaccc ataaaattgc taagaaacaa gggtgtccat ttagtccaat tacgtacttt
     6181 catctgtctt ggttatctta cattttaatt ttgaacaaga caaaagtacc tatatttttt
     6241 tatatgcaat aaaaaaatta gtttctgata caagattgtt tgaagtatag tatcataagg
     6301 attacacaac aaacaagcat aagaataaga acggtattta taatattaac agtagcatgc
     6361 acaaaaacat cgcctgcaaa aaatgaaata cccgataatg cggcaaaaat aaaaaataat
     6421 cctaacatat aaataatcca attcaacgaa atggaatggg tataggaaaa atcattggca
     6481 atattttctt tatacattag gtatcgttta atgcctaaga tgacaattaa aatagcataa
     6541 acacaataac ccaaaaatcc agccactcta atccagcttt cagccgaatg taattgtgca
     6601 ataaaatcag agacggaata aacaaaaggc aactctccct gaataagaac gataattatc
     6661 caacttataa gcaataaaaa tggcaaactt aaccatgcaa tccacttctt aaaagtaagc
     6721 cctcgctgac ttgtaactac cactatatag ttgtatataa atggaaacgt gattaatcca
     6781 gaaagagaaa aaaagaagtc gaatactgga tacggtggat cagcaacaaa aacctcccac
     6841 acaacaagag caagcaacca aggtaaccat gcaatcagaa atatcaaaat tgcttttttt
     6901 tgtttatccg atctccactg agggatgata gcaattaaga aaataaacat cacagtcatt
     6961 atcaacggaa ataggtagga aaacaacaca aaaaacaagt ctaatgaacc attcagcata
     7021 aaaataagat taagaaaata acataacgtg tatgttcaag tattgtaaat cgaataaaaa
     7081 ataataccac ctacacatag caaaagaaaa gtacaaaaaa aaatccgaac tagctagttc
     7141 ggattttcta taataacaca acaaatactt actcagcaat aacctctaaa ctaatttctg
     7201 aagtaacttc tttgtgtaat tttaatgtag ctttataagt acctacttct ttaatagcat
     7261 ctttaagtac aatgatacgt ttgtcaacaa caataccttt agcttctaaa gcctcagcta
     7321 cttgaatagt agtaaccgaa ccaaagattt tacctgttga actagctttt acgccaatag
     7381 ttaaggtcaa accttctaat tgagcagcta tttcatcagc ttgttgtttt aattgagcta
     7441 atttatgagc tctttgtttc aaattttctg ccaatacttt ttttgcagac gatgtagcta
     7501 aaacagcttt accagtagga ataaggaagt tgcgaccata accatctttt acagttacga
     7561 tatcatcttt aaatcctaaa tttattacgt tttcttttaa tataatttcc attgtttttt
     7621 tcctcctttg atttattatt tcatcatatc ggttacatat ggaagcaaag ccaaatgacg
     7681 tgctcttttt acagcttgag caacttggcg ttgaaacttc aacgaagtac cggtaagtct
     7741 gcgaggtaaa attttacctt gctcgtttaa aaactttttc aaaaactcag gatctttgta
     7801 gtcgatataa cggataccac tttttttgaa acgacaatat tttttctttt tattgtcaac
     7861 tgatggaggt gttaaatatc taatttctga tgaattttgt gccatggttt agttctcctt
     7921 tacttcttta gttgatttta attttcttct tttctcagca tactcaacag catatttttc
     7981 catttttagt gttaaaaaac ggataacacg ctcgtcgcga cggaattgaa tttccaactt
     8041 gtccacaata ggagactcag cattaaactc aatcaggtta taaaaaccag ttgatttttt
     8101 ttggattgga tacgctaact tgcgtaatcc ccaattttct acgttcacaa tctcagctcc
     8161 ttcttgttcg agaagagact tgaacttttc aaccgcttcc ttcatctgaa catcagacaa
     8221 aacgggagtc aaaatgaaaa cggcttcgta atgattcata cattctctgt ttaaattgtt
     8281 aataaaaatt tttagcggga caaaggtagt gtttttttat gtttcgacaa atattataga
     8341 aagtttttgt acctttgtgc ctcaaaataa tacaaatatg gcagtaaaag ttggaaataa
     8401 agccccaaat acattgggaa aagacgaaaa cggtaatctt attcaactat cagactatca
     8461 agggaaaaaa gttattttat atttttatcc aaaagataat acctcgggtt gtactgctca
     8521 agcatgtagc ctacgcgaca attattctga cttaaaaaac cttggctatg tggtaattgg
     8581 tgtaagtatt gatagcgaaa cttctcatca aaagtttatt caaaaatttg atttaccatt
     8641 tccattaatt gctgacaccg ataaaacctt ggttgaaact tttggggtat gggcagaaaa
     8701 atcgatgtac gggaaaaaat atatgggcac agtacgtacc acttttatca tcgacgaaca
     8761 aggcattatc acacatataa tagaaggtaa ggcagtaaat acaaaagaac acgctactca
     8821 attaatccaa cttattcaat cataaacata aacgctataa aaaccgcatg attcaatttt
     8881 ttgaaacccc tgaaaaaaat atacttgctg ttcaatacac aaaagaatta agcaaagaag
     8941 acaataacaa gttagtttgg ttatttggaa atgctaacat attggataac agttccatca
     9001 ctggaaattt tgttggacca agacgcgaaa tgattacgcc atggagtaca aatgcggtgg
     9061 aaataaccca aaatatgggt atcgagggca ttgttcgtat cgaagagttt tatcctgctg
     9121 atactacagg cgattttgac ccaatgttgc aacgcaaata cgacaaactt actcaagaga
     9181 tttttgatgt catcaaacaa ccagaaccca ttgtttttat tgatgatatt gctgcttaca
     9241 accaacaaga aggtttggca ttaaacgatg acgaaattaa ttacctcaat aacattagcc
     9301 aaacaatagg tagaaaacta accgatagcg aagtttttgg tttttcgcaa gtaaattcag
     9361 aacactgtcg ccataaaatt ttcaacggca aatttgttat tgacggagaa gaaaaagaac
     9421 tttctctgtt taaattaatc aaaaaaacat ctgaatacaa cccaaacaaa ttagtatctg
     9481 cttacaaaga caatgtagca tttaacgaag gtcctgtgat agaacaattt gcccctaaaa
     9541 gtggcgataa accagattca tttacaacca aagaaattaa atctgtatta tcgttaaaag
     9601 cagaaacaca caactttcca actacggttg aaccctttaa tggtgctgct actggcacag
     9661 gaggagaaat tcgcgaccgc ttgggcggag gaaaagctag tttgcccata gctggaacag
     9721 ctgtatatat gacatcgtat cctcgcatcg aatcaaatcg ttcgtgggaa gaaaatataa
     9781 aagaacgcaa ttggttatac caaacacccg aacaaatatt aatcaaagca tcgaacggtg
     9841 catctgattt tggaaataaa tttggacaac cgcttatttg tggttcagtt ttaacgtttg
     9901 agcacgaaga aaacaataaa aaatatggtt tcgataaagt aatcatgttg gcaggtggtg
     9961 ttggttttgg taccatgcgt gatgctctaa aaggcgaagc aaaagttggc gataaagtag
    10021 tagtgatggg cggcgacaat taccgcattg gtatgggtgg tggtgctgta tcatctgttg
    10081 atacaggtca atacgacaat gccattgaac taaatgctgt gcaacgtgcc aacccagaaa
    10141 tgcaaaaacg tgcagccaac gttatccgta ccttagcaga aagcgaccgc aatcctattg
    10201 tttctattca cgaccacggt gcaggcggac acctaaactg cttgtcagaa ttagtagaaa
    10261 gtagtggtgg aaaaattgac atgagcaaat tacccatagg agactctacc ctatctgcca
    10321 aagaaattat tggaaacgaa agccaagaac gcatggggat gctaatggac gaaaaagaca
    10381 ttgacctagt gcaaaaaatt gctgacagag aacgtgctcc gatgtacgtg gtaggcgaaa
    10441 ctacaggcga tatgcaattt gtatttgaac aagcaaatgg cgaaaaaccc attgatttgc
    10501 gtttggatta catgattgga aaccctcctc gtatggtaat aacagacaat acccttaacg
    10561 aaacatttga agaagttagt gtttctgata gcaaaattga agaatatatt accaatgtat
    10621 tgcgtattga agccgttgca tgtaaagatt ggttgaccaa taaagttgac cgctctgtaa
    10681 caggaaaagt agccactcaa caatgtgctg gagaattaca attaccatta aataacttgg
    10741 gagctgtagc cttagattac caaggaaaag cgggtatagc aacttctatt ggtcatgctc
    10801 cattagctgc tttggttaac ccagaagcag gctcggtatt agctattgct gaagcactta
    10861 ccaacttggt ttgggctcca ttaacagaag gcttaaccag tatttcatta tcagccaatt
    10921 ggatgtggcc ctgcaaaaat agcggagaag acgctcgtct atacaaagcc gtagaagctt
    10981 gtagtgattt cgcttgttcg ttgggcatca atatccctac tggcaaagac agtatgtcga
    11041 tgacacaaaa atatggcaat gataaggtct tttctcctgg tacggttatt atttcggcag
    11101 cagctgaagt aagcgatgta acaaaaacca tttctcctgt tattatcaat gatacaaaaa
    11161 ctgctgtata ttacgttgat ttttctttcg atgcactaaa acttggaggc tcggtactat
    11221 atcaaacact taacaaatta gggaatgata cacctaccgt tcaagacagc gaatattttg
    11281 caacagcttt tacagctgtt caagaattga ttgccaataa gttggtgctt gctggtcacg
    11341 acatctccga aggcggtatg atcactgcat tacttgaaat gtgttttgct aataccaaag
    11401 gtggtttatc tgtcaatcta tctgaattaa aagatttctc gcttacaacg gttttatttg
    11461 cagaaaaccc aggggtttta ctacaagtta aaaacacagt tgaggttgaa aaactattaa
    11521 cagaaaatgg cattggattt gccaaaattg caacacccat taatgaccgc aaattaatac
    11581 taacaaaaga tagcacgcac caagaattat cgattgatac acttcgtgat gtatggtata
    11641 aaacatcgta tttacttgac agaaaacaaa gcggagaggt ttgtgccctt caacgttttg
    11701 aaaattataa aaatagtcca cttcagtttg cctttggcac agaatttacc ggaaaattag
    11761 caaaatatgg cattagcaac gacagccgcg gaaaaagcgg agcaaaagca gctattattc
    11821 gtgaaaaagg aacgaacgga gaacgtgaaa tggcgtatgc actatactta gctggttttg
    11881 atgtacgcga tgtgcacatg acagacttag ccaatggtag agaaacgctt gaagatatac
    11941 aaatgattgt attctgcggc ggtttttcca actcagatgt attgggttcg gcaaaaggtt
    12001 gggctggcgg atttttatac aacgaaaaag ccaaaacagc actggagaac ttctacaaac
    12061 gtccaaacac tttgagctta ggtatttgca atggctgtca gttaatgata gaactaaatc
    12121 taatcaatcc agagcataca aaacgttcgc gtatgttgca caacgattcg cataaatttg
    12181 aatcaaactt tgtaggctta catatccctg agaacaattc ggtattattc aattcattgt
    12241 cgggtagcaa gctagggatt tgggtagcac atggcgaagg aaaattctct ttgcccgaaa
    12301 gcgaagataa ataccatgta attgcaaaat acggctacaa cacttatcca gccaatccga
    12361 atggttctga ttacaatgta gcaggtatct gctctgccga tggacgacat ttggctatga
    12421 tgcctcactt ggaaagagct attttccctt ggcaatgggc atactaccct atcgacagaa
    12481 aaacgactga tgaggtatcg ccttggatag aagcttttgt aaatgcaaaa aattggattt
    12541 tagcacataa ataaaaaaaa gacaaagaca gtacatgatg atactgtctt ttgtttatag
    12601 tagaatttac gagatgaacg cttggaaact atttagtaca tttttcaaaa ttggcacctt
    12661 tacctttggt ggaggatatg ccatgttgcc attaatagaa caagaagtgg tacacaagca
    12721 acaatggcta aacacagaag aatataccga aatgttatct ttggttcaaa ctgcacctgg
    12781 accaatttct atcaatagtg cggtatttat tggctacaaa cttcatcgtt ggaaaggagc
    12841 cctatcttgt ttgcttggcg ttattctgcc ttcgtttatt attattttac taattgcggt
    12901 tgcctttact gaaataaatt caaacccgat tgtagaacgc atatttaagg gtatccgacc
    12961 tgctgtagta gccttgatag ctgtgcctgt aattaggatg atacaacgcg aaaaactcca
    13021 ctggactagt ggacttattg ccctccttag cattatacta gtatggttgt taggttattc
    13081 acctgtgtgg gttgtactag gagcaattat ctatggcatt gtaaaagaat accttaccac
    13141 aaaaacgaaa aagccatgat ttatttagaa ttattttgga gctttttcac catcggactt
    13201 tttggttttg gtggcggcta ttctatatta tcgcttatcc aacacgaagt agttgatata
    13261 caaggatggt taacaattgc cgactttacc aatatagtag ctatttcaca agtaacccct
    13321 ggacccattg ccatcaattg tgccacctat gttggttaca cagccaccaa ttcgatactc
    13381 ggatcggctg ttgccaccat tgctgtatcg ctaccctcac tatttattat ggttttaatt
    13441 agccgattta tcaatgcttt tagaagcaat ccgtatgtag cagcagcctt aaacacatta
    13501 aaaccagcca ttattgggtt aattgcctca gcagccttat tgcttagtaa tccctccaac
    13561 tttattgatt acatcagttt tatcattttt ggtaccgtat tcgtggcagc ttattgtaaa
    13621 gtcaatccaa tttttttaat ccttctggct gggatggctg gattggttat atattaaaat
    13681 ttacttcccc atatacaact caatagcttt gcttctgaat aaaaactcaa acatggcttg
    13741 agtttgattg gataaagcct tggttaatgc attcttagcc tcattatatt cataaatagt
    13801 ggagactcca ctatcaaatt tttctgtctg ataatcaaat gatttttgca ttgcacggac
    13861 attattttca gagacactga ttttttcttt tgccgccaca gttaacaaat aagcctgctg
    13921 taactcttgt cgaagttgta ttttagtgtt tttagcaact aattcctgac tctttacggc
    13981 aagttctgat tgttttactc tgttaatcac agacatttta ttgaaaatag gtatttgaag
    14041 attcagcgtt actgcaatat tttggttttg ttgccattgt tctgaaaaag gtaaattaag
    14101 cctgttttca gcattataat acccattgcc ataactagca cccatagata atcgtgggta
    14161 ataagccgat ttttctatac gtacttgttg attagcactc tctaatcgaa gctgttccgc
    14221 ccttatagaa ggcatggtag caatcgtact tgtatataca ttttcaaaca tcggaagcaa
    14281 atactcctct cttgccacaa tctctacatc ttcaatatca aagctatcca catcatcaat
    14341 taaaaggagt tgagcaaact gcatcaacaa attattaact tcatgctttg cttgtaatat
    14401 agcaagttct tcttgcgata attgagcctt tacttggtat atttcgcctt caggcaacct
    14461 tcctgcgtct attaaagctt tcgttttttc aagttgattg ctagtcaaag catattgatt
    14521 ttcagctaat tctacctgtt tctttgcata tgacacctga aaatagtgca aaagaacttg
    14581 taattcaatc gtactttttt cttgttctac atctagtaat agtgcctgaa aatcaagttt
    14641 tgatttctca atagcataaa acatcctacc accatcatac aaatttaaac tacttgataa
    14701 tgaaaaattt gtattctgga cattattatt ttgatacgtg tttgtttcat caatagatcg
    14761 accaaaacta aagctttgac ctacatacat atttaaatcg ggtaataaat tttgtttcga
    14821 ttgctgtaga ttaatttttg cattttctgc ctgtatcaat cgctgttgaa cttgaatatt
    14881 ttctactttt gcatgctcta aacattgctc aagagtccat ttttgctgac caaatagtat
    14941 actactaacg aatagcaacc atataactaa tagtggtgtt ctcatatttc tatttaacaa
    15001 gtttaatctt ttcattttgc gttacgcctt ccaacacttc aatattaata ccatcagata
    15061 aacctaattt gacagctctt ttatcaaata ctaatgtatc ttttgtttga acctcaacaa
    15121 atacactatc tttctcaaaa atcaaagcac tttcgggcaa agccagaata ttttctttct
    15181 tttctaatat aatctgtgca ttagaactat accccgaccg aacaaaaaca tcatcagaca
    15241 atgacacagc cgctttaatc tcaaatacaa tagctccacc atcgcttatc ccttgtggtg
    15301 caatatactc taatgtagca tcaaatgttt taccttctaa agctccaata actagcacaa
    15361 taggcattcc aacctttatt ttacccactt cagtttcgtc aatctttcct acaaaaatca
    15421 tatctttcat attggcaact gtagcaatag tggtgccatc attaaaatta ttactttgaa
    15481 taacagaact acctaccttt tgaggaatat ctaaaatcat gccattgatt gtggaacgaa
    15541 taagtgtatt actgtaatta cttgcattac gtgttatgcc ttctttcatc aaatcgtaat
    15601 tattttgagc atccactaaa tctacttgac tagatttaag ttcgatagta gctgtttgat
    15661 aagcttcttg agaaataacc cccttatcaa acaaagattt ttgtctatca taatctattt
    15721 gagccttttc aagtgctatt tgagcccttt taatacgcga ttcggcagca cttaatgaca
    15781 ccatatcagg aataatttgt accttagcta ttacatcacc tgctttcact tgttgcccag
    15841 cctctttgta tatttcagta ataatacctg atatttgagg tttaatttga acaaccgtac
    15901 gtggctcaac ctttcctgtt gctacggttt tattttcaat agtggtaaca atcggagatg
    15961 ttgttgaaaa gctagttttc tcaggctgtg attttttcca caacatgtag aacgtgccca
    16021 atactaacac aaccaatatg acaattgcac taatttttaa aaactttttc atatcttttt
    16081 atagttaaaa tgttacattt tattcttctc ttattgcatc aatgggtttt atccgaatag
    16141 ctcttcgggc aggaataatt cctgcaagca caccaaatag cgacataata cctgtagcga
    16201 taatcgcaac tccaaaatcg atatgtggat tcttaaaaaa catgccatca gcaggaatta
    16261 atccattggc aaaatgcaat atccacacac ccagaactaa gcctcccata cctgctaata
    16321 cagtaagtac caaactttct accaaaattt gaccaataat aactttggga gtggctccaa
    16381 tggctcgcct aattccaatt tcttttgttc tctctctaat ggtaataagc ataatattgc
    16441 tcacacctat tataccggct aacaaggtac caattcccac tatccaaatt aatagttgaa
    16501 taccgagcaa taacgctcca atttgttgaa attgttgttt catattaaaa ctccaaatag
    16561 cttcattatc agacggagcg atactattac gagccttaag tacctcttta gtatcttctt
    16621 ctactttgga tatatctgcc caatcttcgg caactacaga gatatatgat attcgatctc
    16681 ctaaattaaa tacttgtttg gcagtagtat atggcacaaa tacggtattt tctggctgac
    16741 taccaatatt tatttgagtt gctgatttaa taactcccac tacctgaaaa cacacaccat
    16801 ttacacgaat atatgtcccc acaactgttt catctgctaa aaaaagttgt tcttttacct
    16861 tttcccctat aacacaaatt tttcgtttgc ctagttcatc taatttatta atgagatgtc
    16921 cttgcaacaa aatctgagga ttcaccaaat acaattccgg agtaattccc tgtacagaga
    16981 aagatcccac tttcatacca ttcatcacat tattctccgt agtatttgtt ggaaaaacgt
    17041 aagggacagt aaatttaact ccctctacat ttttttcaat cgccaccaaa tcactcgatg
    17101 taatatacca cgaacgtcca cgctgaaacc ccttatatgg ttcagtagta gtcccagcaa
    17161 acataaaaca tgaatttctt tcaaaatttt cagttccatc taatatccca ttctccaaag
    17221 ccttgccaga accaagcata ataaccaaca tcagtattcc ccaaaacaca ccaaacgcag
    17281 tcattacact acgaagctta ttttgcttta tagtatgcca aatttcttgc catctgtcaa
    17341 tatcaactat acgcatatac ttattcgtct ctcaaagcat ctataggttt aattctaata
    17401 gccttacgtg caggaaaata acctgcaata acacctgcaa taatgagcaa aacagtagcc
    17461 accataacta aatcaagttg aatagtgggg ttataaaaaa agactgcacc tgtttcatcc
    17521 attggcattt gcttcatcac ataatttact ccctcggtca gaccaacacc aaacaccatt
    17581 ccgatataac caaaaaaagc cgttataata atcgattcta acacaactaa cacaataatt
    17641 gaagatggtt tagctcctaa agctcgtcga ataccaaatt cttttgttct ctcttttacg
    17701 cttattaaca tgatattact aataccaaca attcccgcaa taagagttcc aacaccgata
    17761 atccacacaa aaatagatat cccagcaaat aacttttggg taaacaaaaa actctctagg
    17821 gtattccaat accccatcgc tccttcatct tctacatcaa attgatggta tgatgcaaaa
    17881 cgttgcttaa tattatccat aatttgcttg ctttcatcaa cattggtttc gttttgcata
    17941 tgtaaattaa acgatatttc atgtatctta tctccaaatc catacaaact ttgagctgta
    18001 ggaagtggaa tatacactaa tttttgccag ctacgaggca cttctttaaa aacgcctata
    18061 acggtaaaca tgataccatc tatactcaat tgtttaccta acggggactc gtctttaaaa
    18121 aaatcctgaa caaatttgga acttacaacc actacttttc gtttatgatc acaatccaat
    18181 tggttaacaa aacgtccttg attggggagc atctccaatg cttcaatagg cacaaactcg
    18241 ggcaaaacac cacgcacatc gtgaattcct ttttcatttt tgtacatcac atttttccct
    18301 cgcatatcaa tcaggggcga taattctcca atagatgcat caaaatcttt cctcaatatt
    18361 gtttcgttcg tattcgtaaa ctgaatattg cgttttgtag gaagcccttt gtatgccaaa
    18421 ttagttctac cagaccacat ccaaacagcc aacttggagt catctttata attcaatgcc
    18481 attccattct ttaagccatt acctgctgcc agcaaaacaa tgagcataaa tataccccat
    18541 gccacagcaa atccagttaa agctgtttta agtttatttc tacgcaaaga cgcaaaaatt
    18601 tcttcccata attgaaagtg tatcatatct tactgatgta tatcttgagg ctgaataata
    18661 ccatctttca ttcgtataat acgttctgtt gaggcagcta cttcattttc gtgagttacc
    18721 actatcacgg ttacaccctg ttggttaatt gcctttaata agtctataac ttctactgaa
    18781 gtctgactat ccaaggctcc agtcggttcg tcagctaata ttactcttgg attagaaata
    18841 agtgcacgag caatggctac acgctgtttt tgtcctccag aaagctcatt aggataatga
    18901 tgagcccaat ctttcaaacc cattttttcc aaatactcca atgctatagc attacgcttt
    18961 ttccgagcta tccctttgta gtaaagtggt aaagcaacat tctctaatgc atttttaaaa
    19021 tgaattagat taaatgattg aaaaacaaac ccaattaatt cattacggta ttgagcggct
    19081 tgtgtttcgg ttaaattctt gataagttga ttgtctaaat aataatctcc ctcgtcataa
    19141 ttatccaata tccctaaaat attcaacaaa gtagattttc cagaaccaga agcccccatg
    19201 atggaaacaa attctccctc tttgatgtgt aaatcaactc ctttaagaac ttttaatgga
    19261 gaaccattgt aatatgtttt gtgaatattg gtgaggcgta tcataggaat aaattatcta
    19321 aaaaacgtct aaaggtaaaa tttcttatct gaaaaataaa atattttata caaaaagaat
    19381 atatctttat ataaacaaac taattatacc cctataattg agaaaatgtt ttttaatatt
    19441 ccctctattt cttttattgc agaacggtta gtcagcctta ctagttgtct gcgacaccaa
    19501 gcttgacaaa aactccaagc agaaacactc ctaaaaaaca cccaaatact aaaaagaaga
    19561 gatatgccat aacaaaccaa ccaagaaaat ggaacttcta gtaaaaatag aataaacact
    19621 aaaaatccat tccataatgg aaaaataaat aaagctccta gagctaattg caaagaaact
    19681 tttagcaact catctcccgc atttctacta catacactag ttaataaaaa agggataatc
    19741 tgatgaacaa atgcatatat ctgtatggga atcaatagta tcgtaataaa aacatgcatg
    19801 cctcgcttcg caatagagtg ttttttttca cacaccaatt cgtatgacaa atgcaaactt
    19861 acaattagtt catccatgtt tttagtcaac ttcatcaact ctacgtatgt tgtttcttga
    19921 tgtactttaa gcaattctac cctacttaca acagcttgtt ctatagccaa atcagtagca
    19981 taacgctctt gaatagctgc attatttttt cgatatatac gagctatcgt acacagcacc
    20041 aaatattcgt tgtaatatgt tgtttctttt atgtctagca tattactact catagctctt
    20101 gctgtttgtt caagcaactg ctgttgtgct ttttctggct cttgcatata gagttcttga
    20161 aaagtaagaa cagaaatggg tttgccaaca cacaccgaaa ccttactctg aaacaatcct
    20221 cctatatgat gaatacccac gggtaataca aaaatttctg tttcaaaatt agcacaagag
    20281 gctgtttgaa aggctatccg agcaaatcct tttttcagtt ttcctaattg ttccctttct
    20341 tgatgccccc cttcaggaaa aagtgctaaa atgccattat gctgagcaag taactgagcc
    20401 gtagttgcaa acactttttc attttcaagc accctatttt tacctacatc ttgcatacga
    20461 aaggctggta gtaaacgaaa aaaaactaac aattgtgcaa catacttatt cttaaacaca
    20521 tcagcacgtg ccataaacac cggatatcta ccatctttaa tcataaacaa taatgccaga
    20581 gcatccaaaa ttccatgttg atgattggct accataataa atggcttgcc agtaggtata
    20641 ttttctgcat tatatacagt atatgaacga tgcaatatat aagcatgtat acaccgaacg
    20701 tatttaaaaa tcagtttgta taaaaaataa gtacgaaaga tacgatgata attcattcgt
    20761 taattgttct aataaaaaaa caaagataca gtattcagac aactcgtata cgcttaaaat
    20821 aagaaatatt ttctactttt gtacaaaaat aatgttatgc aacaaacaga atggtttcct
    20881 atagtaaccg aaactggcga aagaattggt aaagcaaccc gaaaagagtg tcattcaggg
    20941 tcttttctcc ttcatccagt ggtacacctt catgtattta acgccaagaa acaattattt
    21001 ttgcaacaac gtgcagccaa taaagacatt cagccaaata aatgggacac tgccgtgggt
    21061 gggcatgtag atgatggcga aagcatacag atagccttag ttcgcgaagc gtatgaagaa
    21121 ttaaaaatca aagatttttc ggcaaacttt ctcttccagt atccctttcg ctcacaagta
    21181 gaacacgaat tggtacatag cttttgggtt caatacgatt ttcccattca agccaactta
    21241 gaagaaattc aagatgctag gttttgggtg atagatgaca ttaaaaattc tttggggaaa
    21301 caattgttta ctccaaactt tgaagatgag tttaccaagc tactatcgca ccctgatatc
    21361 atccaatttt tacatcccta aaaaaaaaga gaagcctcct atctaagact tctctttttt
    21421 atttatttca accatcactg ttgattttaa aaaccttggc tattatcgcg ttgtcttgaa
    21481 acgtaattaa tctttaaggc atacgctcta cgcaaagact gtttaatgtc tgttacaatg
    21541 cccatcttag catcttcgtc aactctaaga gctacagtta gtagcgattg ctcatactca
    21601 ttcatagccg cacgttgagc tacaatataa tcttcgattt gcgatacatc agcaaaagca
    21661 tcatcaagtt gtacacgcgt ttcagaccca tatacacctc tatatttatc agtcggagta
    21721 cctacataaa taaagcgaac cagattcttc ttgtccaatt tttccaactc agtagcctta
    21781 ggttccttaa tggacacctt aaagtcaacc tcttttaatt gtgtggttgc cataaagaag
    21841 aacaacaaca taaaaataat gtcaggcaat gatgcggtac taatagtagg caattctctc
    21901 gaactactat ttttattaaa ttttcccatt acttattccc tccataattt tttggttccg
    21961 cttccgaaat tttttgtgga taaatgtatt gtatggctgt ttttctatca tcatctagct
    22021 ccgcatacga tactccaaac ttcgatttag ctaattcttc acgcaattca ttatatgcag
    22081 ctataagctc attttgcact tcaatgtatg ctttatacga agtagttcta tcattttgaa
    22141 gagaaatcac atgatcccgt gtaagaggaa cttctccaaa aaattgaact tcttcagctt
    22201 ttttttcagg catatcttga gcattattcg catttacgat aaattctttt gctttcttac
    22261 gcaattcagt tacactgata tattcacgtt gcaccatcaa ttgattatcc gaattcagca
    22321 acactaccaa tacattacgc tcttttacct ttaaatcttc tgttttttgg tcaggcggaa
    22381 tgggtggagg caatcttcta gcaagccctt tatttaccac catagtagta gttaccaaaa
    22441 agaaaatcaa gagcaggaag gaaatatccg ccatcgaact tgaattaatt tcaggtactt
    22501 ttcttttatt tctactcatt ctatttatta atttaatatg attcgtgtat tacaacttat
    22561 tttaatcttt ttgtaaagaa gcttaaaaac attgacaaca ctgctacacc cgacaataga
    22621 tagattgctt ttatttgcac atctacaaac ttaatttcat tttcagtagg ctgagcatcc
    22681 aaacctaaaa tttgcatcgg gctgccatct gctattaacc aagcaaaacc taccaatgct
    22741 aataaagcaa aaatactaat taatggcttg gttgcggttt ttttatctac tttaaaattt
    22801 tccaaaaatg caataagtag cgatacaagg gtaataagaa tagctatacc aaacaacaca
    22861 tacattaaaa tcatcattgc ttgtgtataa tttggttcta tgtattcagc atattcatct
    22921 actgctccac cccaaaagaa caaaacagaa actaccactg tgatgcctat taggacatag
    22981 agggttattt tcgaaagatt gtttaacatg tttttatttt ttaattgtaa aataatctat
    23041 tttttattgc tttgttcgac taaaatatct aacatagaaa ttgaagaatc ttccatatcg
    23101 cttactactg attcaatacg cgaaacaata aagttataaa atacttgtaa aataatagct
    23161 acaataagac ctccaacagt agtaatcaaa gctaccttga tacctcctgc tacgatagtt
    23221 gcagaaatat cacctgcttg ttgaatacgg tcaaaagctt caatcatacc aattactgtt
    23281 cccaagaatc ctaacatagg agccaatcca ataaacaaac ctatccaaga taaattcttt
    23341 tctagtaaag ttgtttgaac acttccataa gaaataatag atttttctac tacgtcaata
    23401 ccttcgtcaa tacgtgttaa gccttgataa aagatagaag caactggacc tcttgtattg
    23461 cgacatattt ctttggcagc ttctacacct ccttgtgcca atgcagcttc aacttcttct
    23521 aataacttct tagaatttga agtagctaag cttaaatata taatacgctc aatacatacg
    23581 gctagcccta aaataagaca taatgcaatt gatgccataa aaccagcacc accttcgata
    23641 aatttttgtt ttaatgcttg gtgaattcct ccttcttcaa ctacaggagc aactgtttct
    23701 tctactactg cctcatctgt ggctacttgc tctaccgatg ttgagtcgac cgccggagct
    23761 gcatcttgtg ccaacgctga gaaagtcaaa ccgaaagtaa gactaaaaat tactgctaaa
    23821 actgcaaaca actttttcat tttttttttt gtgttttgat taattacttg ttttatttaa
    23881 aatgattaat gtattccctt tattaaataa attacgtagt ttttacttat tctttctaaa
    23941 caagagaaaa actaaaaaaa cattcagggc tcaaagttaa taaaaaagaa agaatacaaa
    24001 acaattttta tgcaattatt ttgtctaaac aagtataaaa aagaagagca tttaatttac
    24061 accaaataca aagaaaagaa attaactaaa aacagtaaga gccccagtaa acactagaac
    24121 tctattattt tatcagggta agggaggctc tccctgttaa tgtagacaga cagggcagtc
    24181 tacccaattg tccaatcgtg gttaattctc atagcctaat tggagcatac aacgcaaaaa
    24241 ttgtccgggt tcctgttttt ctcccatata tttctcccaa atttctcgtt tgtaaaaaat
    24301 gccttgttac atacttttgc acaattacct aattttcgca ccgttgaaaa agtaaatttc
    24361 tttctccgta atcctaaaat gccttgttgt tttcacaaca aaaggacaga aataaacccc
    24421 gactttcaaa atcctaattt aaatacaaag ataagagcgt gttagtgact ggtgcttggt
    24481 ttttagtcgt tttaattatc tattttcaat ttaaataacc ctgtcagtgc catattaact
    24541 gtcaaataaa agtcatgaaa tataatttca taatagtcac atatagagat tctactgtcg
    24601 taattaaacc ttttccaata gaaaagtcaa aggctttcaa tcgttacata gataaaatta
    24661 ctaaaagtcc aaaagtttat tattggagag ttacagaatg aacagaccga acgcaaaaag
    24721 aatttgattc tcattgatag gaatgttctt tattcctttt aagaagttct ttcccgtatt
    24781 cagaaagccc ataaagtctt tctcctgtaa ctcttgcact ttctttaaaa ataattatgt
    24841 cgttgtcaag taaataagtc tttaaaatgc ctatttcgat tgcatcttga tatttaaact
    24901 catataattt tctattttca acttgatagt ttcgtaagtc tctttcaatg tcaaatatag
    24961 aatcctcact taggtatctt gaatgttgtc tgaaaaattc aagagcatgg tattttatta
    25021 attcatctgt cataattatg ttatttaata tgtctatatt ttttcggcac ttgcccctaa
    25081 catcagtctg tctaaaaacc tccgtttagt ttaatttttg ctcaaatgta atgggtttta
    25141 acgaactttc aaaatatact agggattttt ggctaccatg cttggggtga gcgtagtgtt
    25201 gttgttccac acgaggagta tggagagtaa tccttgtttc cattttacac ctttttgggg
    25261 taaaaaatta tgtcaggaca agccacctat caatataaca aaatcaccca tttcaacctt
    25321 tataagggct aaaatgggtg ataaatttgc aacgtgctaa taatcagctg ttattgcgga
    25381 gagggaggga ttcgaacccc cggtacctcg cagtacaacg gttttcaaga ccgccgcaat
    25441 cgaccactct gccacctctc cttatcttgc tttagcaaaa gtggtgcaaa tgtaattgct
    25501 ttttttttct cttgcaaatt tttatattga tttttataaa aaactacagc tattgctcca
    25561 ttacaacaaa taaactacct ttgtatcgta ataaagcaag cacatgtatg atttatcctg
    25621 ctcattttga gacaaaaatt ggatttgata gcattcgagc cattctaaaa caccattgca
    25681 ttagtccgct tggcgaagaa aaagtggatg aaatgacatt ttcggattcg cttgttacta
    25741 ttgatacacg cttaagccaa acaaacgagc tgttaacctt actcaacgaa catgattcgt
    25801 ttccaacaga tgcgtatgtg gatattcgtc cagccttatc gcgtattcgt atagaaggta
    25861 cgtatttgct cgaaaacgaa ttgttcgatt tataccgatc gctgcttacc atacaacata
    25921 ttgtacaatt tatccaacaa aaagatgcca aacgttttga acaattacac ctcttagcaa
    25981 aagatgtctt attattccct cctattacaa aacacattga cacgatttta tcaaaatttg
    26041 ggaaaattaa ggacagtgcc tcgcccgaat tagcctttat taggcgttct aaatcgcaac
    26101 tttcgggcac tatctcacgt ttattacaac aaattattcg caaagggcaa acagatggat
    26161 acattgacaa cgaagtaacg cctgctgttc gcgaagggcg actagtaatt cctgtttctc
    26221 cttcgtacaa acgcaaaatt aatgggatta tacacgacga atctgccagt ggaaaaacca
    26281 tttttattga acctgccgaa gtcgtggaaa caaataatca gattcgcgaa ttggagctac
    26341 aagaaaaacg cgaaattacc cgcatcttgg tgcaatgtgc caattttatt cgtccaatgt
    26401 tacccgattt acttcattcg tatcattttt taggcaccat tgattttatt cgctccaaag
    26461 cactgctagc cattcagcta caagcgacaa aacctgttat cgaaaacaac ccgcacattg
    26521 attggcagga tacgtttcat cccttgcttt acttatcact tcgcaaacaa gggaaagcga
    26581 ttgttccact aagtatcaaa ctaaacgacc aagaacgtat tttggtcatt tctggaccga
    26641 atgcaggcgg caaatcggta tgtttaaaaa cagtaggtct tattcagtat atgatgcaat
    26701 gtggcttact agtgccgatg aaagaatcaa gcactatggg tatctttcag cgaattttta
    26761 tcgatattgg cgatgaacaa agtattgaaa acgatttaag tacatacagt tcgcatttaa
    26821 cgaatatgaa gttttttgcc aaacactgcc aagaagctac tctgctactc atcgacgaat
    26881 ttggcacagg tactgaaccg cttattggag gtgcaatagc ggaggctacc ttacacgtat
    26941 ttaaccaaca aaaagctttt ggtgtaatta ctacccatta tacgaattta aaacactttg
    27001 ccaccgaaaa tgaaggcgtt gtaaacggtg caatgctcta cgataggcac cttatgcaac
    27061 cattatttca attggaaata ggtaatcctg gaagttcctt tgccatcgaa attgcacgta
    27121 aaattggctt gcccgaacat attattgcag acgctacgct caaagtgggg gctgaacaca
    27181 ttgattttga caaaaacctg caagatatag ctcgtgacaa gcgttattgg gagaataaac
    27241 gtcaacaaat acgcctgaaa gagaaaaaac tagaagaaac aacagaacaa cttcgcaagg
    27301 agcttgaaca agttaccaaa cagcgtaaac aagtactgaa agacgctaaa aatgaagcat
    27361 cgttgctttt acaacaagcc aatgcgtcta tcgaaaatac cattcgtact attaagcaat
    27421 cacaagcaga taaagaaaaa actgccattg ctcgaaaaaa gctcgaagaa tacaaacaat
    27481 ccatcaacga cacacagcaa caggacgttt tagaacaacg acttagtgcc atcaaaccac
    27541 taaaagcgaa caaagaaaaa agcgaacaga ctgaaaacaa aacactacat gtaggggatg
    27601 cagtacgcat tatcgacaaa gaaacaactg gcacaatcgt aagtatccat caaaaaacag
    27661 ccatcatcgc cattggtcaa ttaaaatcga ctattaaact cgatcaactt gaacgcataa
    27721 gcaataagca acaaaaacaa ctacaacgcc atattagcta tatcagccaa cgtactcaag
    27781 atgagatgag agagcggaaa ctacaattta aacaagaaat tgatgtgcgt ggcaaacgag
    27841 cagaggaagc tctacaaatt atccgctact acattgacga tgcttttatt gtaaatgcca
    27901 gcagggtacg tatattgcat ggcacaggaa caggagcctt acgcgaagtt attcgtcaat
    27961 acctatcaag cataccggga ataacacgta ttagcgatga gcacatacaa ctaggaggat
    28021 cgggcattac tgtggtagat tttgagtaat gctatttcaa aaaaaaaagc atacaaataa
    28081 gaaataaaaa aagggaacag aatctgttcc cttttatcag tatacgtgaa atagtattat
    28141 ttcaatgtta ctacttgagc agcaccgtta gcaagcttca cgatatatgc accagcagct
    28201 aagctaccat tttgagcagt tacgtcaaca cctgcaaggt tgatgataga gaaatcagca
    28261 tcagcagaaa ttgttccgtt tgcagctact acagcagcat caaatgtaga ttggatacct
    28321 gta
//
LOCUS       DAOSRP010000007        26953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_1987_length_26953_cov_4.281657, whole genome shotgun sequence.
ACCESSION   DAOSRP010000007 DAOSRP010000000
VERSION     DAOSRP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26953
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_26953_cov_4.281657"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLJ82_00980"
     CDS             complement(<1..377)
                     /locus_tag="PLJ82_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HOR40116.1"
                     /translation="MKTFTFGGIHTADMKLSAGKKIQIVPIPSEVIIPLEQHIGAPTN
                     PVVAVGETVKVGQLIGKAVGFISANIHSSVSGKVTKIDTVIDAWGYKRPAVYIAVEGD
                     EWMENIDKKDVVRKEITTKREDI"
     gene            complement(427..1278)
                     /locus_tag="PLJ82_00985"
     CDS             complement(427..1278)
                     /locus_tag="PLJ82_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOR40117.1"
                     /translation="MNTIFLSLVVLGTIGLLAAIILYVVSQKFKVEENPIIDEVEAAL
                     PSANCGGCGFPGCRGFAVACSEATDLSNLFCPVGGNDTMKTVAHIVGLSVVETKPKIA
                     VLKCNGTCEHRPRTNQYDGAKSCKIIHNLYIGETACHYGCLGGGDCEVACAFDALHIN
                     PVTGIPEIDEEKCTACGACVKACPKLLLELRFKGPKGKRIYVACSNKEKGGIAKKACE
                     VACIGCSKCFKVCPHDAIIIENNLAYIIDDKCRLCTKCVDECPTGSIQKLNFPIKKKE
                     VAEQAQA"
     gene            complement(1275..1697)
                     /locus_tag="PLJ82_00990"
     CDS             complement(1275..1697)
                     /locus_tag="PLJ82_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOR40118.1"
                     /translation="MNSSQEYTGTIVKINQETVTVCIKQLAACVSCQTKGACSSADVS
                     SKLIEATINNESFEIGETVRIIGKKNFGLKAVLMAFVIPVFLILLILFVAEYYKKDDL
                     TMVISTLFVLIPYYLILFLLQNKLKDQFMFYVEKIDKK"
     gene            1824..2726
                     /locus_tag="PLJ82_00995"
     CDS             1824..2726
                     /locus_tag="PLJ82_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOR40119.1"
                     /translation="MQQGERYKEIRNVTLIGFVVNLLLTLAKLFAGIVGKSSAMIADG
                     VHSLSDFATDIVVIVFMRVSDKESDENHKYGHGKFETFAILIISICLFVVGIGLLIGG
                     IDKIVYFYKGNEIMQPSLLAFYAALVSIALKEALFWYTFKIGKKVNSDAVIANAWHHR
                     SDAFSSIATALGIAGAIFLGEKFRILDPIASVLVSFFIMKVSYELAMPSVKELLESSL
                     PKEKEDEIIDHVLSIPGVITYHHLRTRKVGNLIAIDIHIKLNKHISFVESHDIATDVE
                     SLLRHQYGNQTVVNIHTEPYEEKK"
     gene            3044..4537
                     /locus_tag="PLJ82_01000"
     CDS             3044..4537
                     /locus_tag="PLJ82_01000"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOR40120.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKALESLGVDVIEA
                     GFPVSSPGDFKSVVEISKAVTWPTICALTRGVQKDIEVAAEALQYAKNKRIHTGIGTS
                     DYHIKHKFNSTREEILERAVAAVKYAKKYVEDVEFYCEDAGRSDNEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPSQYGEKIKFLMENVKGVHNAILSTHCHNDLGMATANTIAGVL
                     NGARQVEVTMNGIGERAGNTSLEEIAMILKCHKHLEIDTQINTQKIASTSRLVSSLMN
                     MPIQPNKAIVGRNAFAHSSGIHQDGVLKNRESYEIMDPKDVGIDENAIVLTARSGRAA
                     LKHRLSILGVAVEGDALDKIYQDFLVLADKKKDVNDDDVLMLAGMERTSNSRIKLDYL
                     QVTSGKGVRAVASVGLDIAGEKFEAAASGNGPVDAAIKALKQIIKREMSLQEFTIQAI
                     NKGSDDIGKVHMQVQYKGSVYYGFGANTDIVAASLEAYIDAINKFVK"
     gene            4595..5989
                     /gene="leuC"
                     /locus_tag="PLJ82_01005"
     CDS             4595..5989
                     /gene="leuC"
                     /locus_tag="PLJ82_01005"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOR40121.1"
                     /translation="MKKTLFEKIWDAHVISEVNDGPTQLYIDRMYCHEVTSPQAFDGI
                     RKRGLTVFRPKQVTCIPDHNIPTINQEKPIEDPISRHQVQALDSNAKEYNLQYFPMGH
                     NKNGIIHVVGPENGLSLPGMTMVCGDSHTSTHGALGAVAFGIGTSEVEMVLATQCIFQ
                     SKPKTMRISVNGNLKDGVSAKDIALYIIAKMTTGGATGYFVEYAGSAVQALSMEGRLT
                     LCNLSIEMGARGGMVAPDETTFEYIKGREFAPKGEEWDKALAYWKSLPTDEGAQFDKD
                     IAFDAADIEPMITYGTNPGMGLGITESIPSLDSIDEAGRISFVKSLDYMGFKPGDKMI
                     GKPIDYVFLGSCTNGRIEDFRAFASLVKGKKKADNVVVWLVPGSWAVEKQIKEEGIDT
                     ILTEAGFVLRQPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGARTILAGPLVAA
                     AAAITGKITDPRTL"
     gene            6037..6648
                     /gene="leuD"
                     /locus_tag="PLJ82_01010"
     CDS             6037..6648
                     /gene="leuD"
                     /locus_tag="PLJ82_01010"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOR40122.1"
                     /translation="MEKFKTISSTVVPLPIENVDTDQIIPARFLKATSKLGFGDNLFR
                     DWRYDKNDQPNPDFVLNNPTYSGEILVAGKNFGSGSSREHAAWAIAGYGFKIVISSFF
                     ADIFKNNAMNNGVLPVVVSEAFLAEIFSSVENNPKTIITVDLQNQTVTNTATNKSEKF
                     DINSYKKECFLQGFDDIDYLLAKKQIIEQYEDKQSDVAYFLTT"
     gene            6661..8163
                     /locus_tag="PLJ82_01015"
     CDS             6661..8163
                     /locus_tag="PLJ82_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOR40123.1"
                     /translation="MIKILDTTLRDGEQTSGVSFAIQEKLSIARLLLEDLQIDRIEVA
                     SARVSDGEFASVQQITKWAKSKNVLDKVEILGFVDGFTSLKWIEKAGGRVINLLCKGS
                     LNHCTHQLRKTPQEHIADIKQVVANAQSMGITVNIYLEDWSNGIQQSSEYVFTLMDSL
                     KDEPIVHFMLPDTLGILNPNQTRTYCSMMVERYPSLQFDFHAHNDYDMAVANVFEAVQ
                     VGIQGLHVTINGLGERAGNAPLASVVAMLHDQLHLSTNINEPELNRVSKLVEAYSGIR
                     IPPNKPVVGDNVFTQCAGIHADGDAKKNLYFNDLLPERFGRIREYALGKTSGKANIRK
                     NLEALGIELDEESMHKVTQRITELGDQKQVVTQDDLPYIVSDIVKEENIAENVRILNY
                     SLSLAQGLKPIANILIEINGETYQEYATGDGQYDAFMKALWKIYDTLGKPHPILLDYL
                     VTIPPGGRTDALVQTLITWDLDGNVFKTHGLDGDQTAAAIKATLKMLNRI"
     gene            8206..8652
                     /locus_tag="PLJ82_01020"
     CDS             8206..8652
                     /locus_tag="PLJ82_01020"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOR40124.1"
                     /translation="MNKQLTIALVAHDRRKADMVEWALHNAQTLSQHKLVCTGTTGGL
                     IQEAFENKSIAADITRVNSGPMGGDAEIAAMVVKHQIDLAIFLIDDLNPQPHEADIQM
                     LLRQCRVHNVPIACNRYSADLMITSTLWDNPTYQPTEPKYVVFERK"
     gene            8726..9799
                     /gene="leuB"
                     /locus_tag="PLJ82_01025"
     CDS             8726..9799
                     /gene="leuB"
                     /locus_tag="PLJ82_01025"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOR40125.1"
                     /translation="MKLNIAVLPGDGIGPEIIKQGLDVTIAVCEKFGHELTYEYGLVG
                     ATAIDQVGNPYPDATHELCMKSNAVLFGAVGDPRFDNNPSAKVRPEQGLLAMRKKLGL
                     YGNIRPVTTFPSLIHKSPLRADLIDGADFVCIRELTGGMYFGRPQGRSEDGTVAYDTC
                     VYSRMEIERVLTLAYSYALKRRKKLTVVDKANVLATSRLWREVAKEMAQNYPDVETEF
                     MFVDNAAMRMIQWPKDFDVMVTENMFGDILTDEGSVITGSMGMLPSASIGEHTSVFEP
                     IHGSWPQAAGKDIANPLATILSAAMMFEYAFDLKEEAALIRKAVDASMDANVRTADIQ
                     VEGKEGYKCSEVGEWIKNWIKNN"
     gene            complement(9883..11667)
                     /locus_tag="PLJ82_01030"
     CDS             complement(9883..11667)
                     /locus_tag="PLJ82_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR40126.1"
                     /translation="MNTFEKLGLEQSILQGISEMGFVSPTPIQEEVIPYMLSNQSDII
                     GLAQTGTGKTAAFGLPLIQLISTKERTTQGLVLAPTRELCMQIATELRKYSTYKEGIH
                     IVAVYGGEDIMKQLKQLDKTPHIVVATPGRLIDLINRRKVSLQGIKHLILDEADEMLK
                     MGFLDDITTIIEETPDSRQTLLFSATMPTEISSIARRYMSNAKEIAVGKKNEASKNVE
                     HVCVVVRKDQKYLALKRILDSNPDIYGIIFCRTRQQTKDIADLLIVDGYNADALHGDL
                     SQAQRDMVMNKFRIKNLQILVATDVAARGLDVNNLTHVINYDLPDDKETYTHRSGRTG
                     RVNKSGISIAIVTPREKGSLSTIEKAINRKFKQELIPTGLEACQKQLNHLIHRMREVE
                     VSEDLDDNYLSEIFEMLTDMSKEEVITRFVSLEFNRFLSYYKDSKDLNNDIKAEKDGG
                     QARNRRTGKSDRIRLKIDQGKNDGYEPKRLLKLINEVTDSKDINIGDIEVTSKFTFFD
                     VDKDQVTRLKTAFATSKFSRSIELNEVKGDRSSRSSSSSPSRRRQAPANQSSSSASRS
                     QSSGRGRDRNKETDKNKKSDRYSKRKTW"
     gene            11947..14280
                     /locus_tag="PLJ82_01035"
     CDS             11947..14280
                     /locus_tag="PLJ82_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOR40127.1"
                     /translation="MKPKTKKTILIGLWTLFASIIVGTVLLFFLIAKGVIGYIPEIEE
                     LQNPKNKFASEIYTADGEVLGRFFYGKDNRVAVNYTQISPYMIEALVATEDIRFFNHS
                     GIDARSLGRAIILRGLLGKASAGGGSTITQQLAKLLYSPNTDNLFQRAMQKPIEWVIA
                     VQLERFYAKEEIIAMYLNQFDFLNNAVGIKSASQVYFNVAPNELTREQAALLVGMCKN
                     PSLFNPNRFPERAKNRRNTVLDQLYKYDYITKQEVDSLKSIPIKLNFQRVDHKLGLAP
                     YFREYLRQTMMADKPQRSNYMSWQMQKYKEDSLAWETNPLYGWCKKNKKPNGSNYNIY
                     ADGLRIYTTIDARMQRYAEEAVKEHIGDNLQKRFFNEKRGRSYAPFSRNITPEEMSSI
                     LTRAKRQSDRYRNMKKANYTNAEIDRAFAKKIPMSVFTWTGVRDTIMSPMDSIKWQKH
                     FLRCGFMSMDPYNGHVKAYVGGPNFAEFQYDMVTQGKRQVGSTIKPYLYTLAMEEGLT
                     PCQKVPNIPQTFYLEDGKEWTPRNASNSKEGELVTLRWGLANSNNWITAYLMKQITPQ
                     ALVRMIHSFGIQSHLEPVVSLCLGPADVSVKEMVGAYSSFVNKGIRVEPLFVTRIEDN
                     KGNVLANFTPQLVEIFSEETSYKMLTLLGAVVNEGTGVRLRYRFQLRGEIAAKTGTTQ
                     NNSDGWFIATTPQLVAGVWVGGEDRSIHFDNIADGQGANTALPIWAIFMKKVYADTSL
                     PYSENVKFDIPTDIRQGYDCRSEPNTEESFDQIFDLE"
     gene            14377..17070
                     /locus_tag="PLJ82_01040"
     CDS             14377..17070
                     /locus_tag="PLJ82_01040"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR40128.1"
                     /translation="MKIHFKLNYFTKWGQNIAIMGSIPELSNNDPSKALYMNFEWKED
                     WSLDIDVQRDAPFELTYKYVLKDTNGLDVLEWGDDRTILVDPLRDENIYCYDSWNPAG
                     AVENVFMTSPFQNVLFKENHIPVPAITPKKYTHIFRVKAPTLKKGEALCVVGSTKELG
                     DWASEKPVVMSNEDKNWWVAKVNLATTKVDVVNYKYGVYDIEDQSLKYFEYGADRKAT
                     VVTTKKSLVIVSDSFARIGSYDFKGAGVGIPVFSIRTKSSFGVGDFIDIKLMVDWAKK
                     VGLKLIQVLPLNDTIGTHTAADVLPYAAISAFALSPLYLNLPKMGKLPADNPLNKAYK
                     TKQKALNDLPLVEFLEIVNFKLAYSKALYLKNKEKFLKNKSYLKFFKENKHWLVSYAA
                     FCYLRDKNGTADHSKWGEYATFSEAKLERLTSPEQAHFDDIAVNYFIQYHLHLQLLEA
                     AEYAHENGVILKGDIPIGVNRNSVDTWVAPELYNMHMQAGAPPDMFAIKGQNWELPTY
                     NWEKMEETGFDWWKKRFQQMGYYFDTFRIDHILGFFRIWQIPLHQEEGIMGYLNPSIP
                     VHINEFGEKGVHFDYNRFCVPFITDAVLWEVFGDDANWVKTNCIDIIDGWILRLKPIC
                     NTQKKVMEMFDKNMITEKIKWGLFDLISNVLFFEVEWSNGMMYYPRYGMQTTTSFRYL
                     DNFTKNKIEELYVDYFYRRQDSFWKYSALKKLPAIKRSTNMMICGEDLGMMADCVTSV
                     MNELGILSLEIQRAPKVDTIEFFHPADAKYLSVVTPSTHDMSTIRGWWEEDREVTQRF
                     YNQQLGHWGEAPYFAEWWVCRDMIVQHLYSPAMWAIFQLQDLLSISDTLRRQNPHEER
                     INVPSNSKHSWRYRMHLTVEELIEQETFNKELKNYIVQANR"
     gene            complement(17137..18216)
                     /locus_tag="PLJ82_01045"
     CDS             complement(17137..18216)
                     /locus_tag="PLJ82_01045"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOR40129.1"
                     /translation="MKRKELPFLFQKLFKNYPFSIVIGASLLLFFLFGGNIWVENSLL
                     YKQIESFFAYILGQTSSSLLRLFGFNVSYNALIQYIGIVGKETFSIQPFVALKYYLFI
                     LFLFSFFFINKNMSLLYIVCGILFLFLLSLFKLAMLLTFDGKLAYQLSDPTNLYIKTL
                     LVVLLLLKFQLFEFSSNYIKSLNDKIQLKFSLSLIGMITIIPMFVNFHKILDLIAPYG
                     TFNWLLNIILWLSQKFMFLIGYSETIIVNNYLYLGINYVGVGAQCLGIGIMTSFSLLI
                     LLIRSSLINKLTYIVFGMFVMIIMNSVRIVFLLLHLYKHQSYQLAMDAHDLSNYFFYI
                     VVFLLFLGYILWFQYVPLFRGKVER"
     gene            18429..19223
                     /locus_tag="PLJ82_01050"
     CDS             18429..19223
                     /locus_tag="PLJ82_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOR40130.1"
                     /translation="MRTLVLFIISLLLFFSCVTPRKTHYFQEPDDVIPSYSEAIFPSD
                     YQIQFNDELYIRVLTLNPDSKRIFNPQLSTTSSSSSGNVTTSPIKGVTSYTVYDDGTI
                     DFPYLGAVSVANKTTREIKVLLEEKLSEYVKDCSVDVRLVNNYVNVLTNTGASRVQLV
                     KEHTNIFEVLAMSHGLSSYAKRSDVKIVRETAQGSTIYSFDLRSKDIIHSDFYYVKPN
                     DVIYVPDFDGQFFRLNNFMTVLTTTTTTISFGFFVWKLIDLWIPKQ"
     gene            19265..19957
                     /locus_tag="PLJ82_01055"
     CDS             19265..19957
                     /locus_tag="PLJ82_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOR40131.1"
                     /translation="MASCITNKSTAYLQEGGNMPQYEETEYTYYRIQPNDELAMQVLS
                     INEEVVAFFKDGQAGATPISYKVYADGTIDIPFISGIHVAGLTVREAAKKIELQVREF
                     APDATVKLGLSNDSFYLITEGKQGKFPMYKEKLNLFQALSLAGNIPINADRQRVRIIR
                     QKDAMSPPIIKEFDIRTESIIHSEFYYIQPNDLIYVSTIKGDFFKVTDYSSTITSITS
                     TLSFLLLIMGLF"
     gene            19967..22366
                     /locus_tag="PLJ82_01060"
     CDS             19967..22366
                     /locus_tag="PLJ82_01060"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOR40132.1"
                     /translation="MENNVPQNNFNEENESSSFDIKIWIIRILKNWYLFFISISMFLT
                     VAYFQNKSWFPIYRSSALVIIEEANRGFNRSQQAFMEGFSVQGGYRNVNNQIVMFSSL
                     DLIKKVVDNLQDLHIEYYTRGRFTSTNLYKKSPVFIEKLFISPNGYEQEFFLEDVGNG
                     KDFLITYAGNKQIAEFTVKGEYGKQLETSLFFITIHKSDFYRNRFQLYFGFRDLDKLA
                     SMLHDRLTFAFKMEGSSVLEVSITGNVHERDCDFLNALCDAFLAEGLARKNDAAIKTI
                     EFIDSQMLTISDSLRVSERALQNFKSDNFMVAANSSTELSAKYRALETKETELRLKDS
                     YIKYLTNYLEKNIEDGSLIAPTSLGVNEPTLTLLVKDFTEVMFKKKEVGERSPLYAKY
                     TRDLSNIKEQMFEALRNIRVGFNIEKADFDKRMAELKAQIQVLPIKEQQLLNFERKFK
                     VNDNYYSFLLQKRADAQILKASNSPDNIILDKARLTGVTNGASKGSNYSTFLLIGLLI
                     PLAFVGLKEFMYPFIRSENEILHLSRRAYQIIGVIRHTSKNIPVIVDAYPRSAMAEIF
                     RVVRTRMEFLNGKKKNMTMMFTSTHSGEGKTYISVNMAAIYAMTGKKTILIDSDLRKP
                     SVSATLSLPENKGLSNYLAGQIDLSEAIIKKSNYKFDIMTVGVIPPNPGELMKSQKMK
                     ELIKELKEIYDFIIFDAPPIGLVADSYAIINEMDLNIYVVRCERTNKPLFKKTITQLQ
                     EDKIPNVHVLFNDINIKKLEYATSYSNYSKYGKSYYTNLLYKNYKDNNGVYFDDLEDD
                     V"
     gene            22415..23710
                     /locus_tag="PLJ82_01065"
     CDS             22415..23710
                     /locus_tag="PLJ82_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOR40133.1"
                     /translation="MNVQDKKIAVIGLGYVGLPLAIEFGKKYRVLGFDIKAARIEELQ
                     AGEDSTLEADLEGMKIATDLYKKNEQIGLSFSSIQEDLKNFNTFIVTVPTPIDEFNNP
                     VLTPLISASRMIGNVLKKGDLVIYESTVYPGCTEEDCVPVLEKQSGLKFNKDFFCGYS
                     PERINPGDKVNTLTKIRKITSGSTPAIAELVDNLYSSVITAGTYKAANMKVAEAAKAI
                     ENAQRDMNISFMNELALIFDRVGIDTNDVIEAAGTKWNFLKYHPGLVGGHCISVDPYY
                     LAHKAKALGYHPEVILSGRRVNNSISEFIAQKTLKLMIQKGHTIKDSNVLILGITFKE
                     NCPDVRNTKVVDIRKELVEFGVNVDMFDPWAEAEEVHEEYGIDLVSKLDETKMYNAII
                     VAVAHDEFKAFDFAKYKKQGAIIYDVKAFVDRDLVDARL"
     gene            23750..24106
                     /locus_tag="PLJ82_01070"
     CDS             23750..24106
                     /locus_tag="PLJ82_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOR40134.1"
                     /translation="MGSLVGEKSYAFALRTIKAYQYIQTINEFVLSKQLLRSGTAIGA
                     LIREAEHAQSKADFLNKMNVALKEANETIYWISLLKDANYIDEKVYNSIQAEATEILK
                     MLISIVKTTKQNLNKK"
     gene            24346..25659
                     /locus_tag="PLJ82_01075"
     CDS             24346..25659
                     /locus_tag="PLJ82_01075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOR40135.1"
                     /translation="MKIAIVGTGYVGLVSGACFAEIGTDVTCVDVDKKKIDDLHNGII
                     PIYEPGLEDIVLRNVKAGRLHFTTELTEILDDVQIVFSAVGTPPNEDGSADLKYVLQV
                     AKTIGENMNNYVLLVTKSTVPVGTAQQVRTVIQTELDKRGVDIEFDVASNPEFLKEGA
                     AIKDFMSPDRVVIGVESERAKEVMTKLYRPFLLNNFRVIFMDIPSAEMTKYAANAMLA
                     TRISFMNDIANLCELVGADVNMVRKGIGSDARIGNKFLYPGCGYGGSCFPKDVKALVN
                     TANEYGYPMRILQAVEDVNFQQKHILFEKLKKHFGNKFADKTVAIWGLTFKPNTDDMR
                     DAPSLVLIDELLQVGCKVKAFDPIITKLTDKAYKDVCIGKDIYDTANDADALVLVTEW
                     QEFRLPAWQVIHKTMKNHIVIDGRNIFDKTEMSQYGFIYEGIGIK"
     gene            25683..26051
                     /locus_tag="PLJ82_01080"
     CDS             25683..26051
                     /locus_tag="PLJ82_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOR40136.1"
                     /translation="MRYIYIFGTLFFTVYGQLILKWRLGNLSFLLPQGNILEKLVSLL
                     KLIFDPYIFSGFASAFIASLFWMAAMTKFQITEAYPFMSLAPAIVFVLGIWLLGETFT
                     WGKVIGLVLIIVGVIITVKL"
     gene            26078..>26953
                     /locus_tag="PLJ82_01085"
     CDS             26078..>26953
                     /locus_tag="PLJ82_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR40137.1"
                     /translation="MQNNIVLSVVSPVYRAENIVQELVRQLHEQIQLITQNYEIILVN
                     DASPDASWEKIIKVCAEDKRVKGINLSRNFGQHYAITAGLSYTKGEWVVVMDCDLQDR
                     PDEIPNLYRKAQEGWEIVFARRTERKDKFFKRLSSRLFHQAYSYLSGINTDRSIANFG
                     IFHQKVIVEYNRMKESARSFPSLVHFLGFKRTTLDVKHAERFDGKTTYTFSKLIRLTT
                     DVILSNSNKPLKMTVKLGFSISVLSFLLALYNIFAHFAGIIKVEGFTTTVFSIWFVGG
                     LILFVMGIVGLYIGKI"
BASE COUNT         9154 a         4178 c         5040 g         8581 t
ORIGIN      
        1 ataatatctt ctcttttcgt tgttatttct ttgcgaacta catctttttt atctatattt
       61 tccatccatt cgtcgccttc tactgctatg tatacggcag gtcttttgta tccccatgca
      121 tctataactg tatcaatctt ggttactttt cctgaaacgg atgagtgaat atttgccgaa
      181 ataaacccta cagctttacc aattaactga cccactttaa ccgtctcacc tacagctacc
      241 actgggtttg taggagcccc aatatgctgt tctaagggga taattacttc actagggatc
      301 ggtactattt gaattttttt tcctgccgat aatttcatat ccgcagtatg aatgcctcca
      361 aaggtaaatg ttttcatacg tcttatttca gaatatacgt aatagttata tactactaac
      421 ttagttttat gcttgtgctt gttcagctac ttcttttttc ttaataggga aatttaattt
      481 ttgaatacta cctgttggac attcatccac acattttgta cacaaacgac atttatcgtc
      541 tattatatac gccaaattgt tttcaataat tatggcatca tgtgggcata ctttaaagca
      601 tttagagcat ccgatacatg ctacttcgca tgcttttttt gcaattcctc ctttttcttt
      661 attactacat gctacataga tacgtttgcc tttgggtcct ttaaatcgta attctaacaa
      721 taattttggg catgccttta cacaagcacc acacgcagta catttttctt catcaatttc
      781 tggtattcct gttactgggt ttatatgcaa agcatcaaag gcacaagcca cttcgcagtc
      841 gccaccaccc aaacaaccat aatgacaggc tgtttctcct atgtataagt tgtgaataat
      901 tttgcatgat ttcgctccgt cgtattgatt agtgcgtgga cggtgctcgc aggttccgtt
      961 gcattttaac actgcaattt tgggttttgt ttccactacg cttaatccta ctatatgagc
     1021 aacagttttc atggtatcat ttccgccaac aggacaaaac aaattactta aatctgttgc
     1081 ttcactacat gctacagcaa atcctctaca tccaggaaaa ccacatccac cacaattggc
     1141 actaggtaat gctgcttcaa cctcatcaat gatggggttt tcttctactt taaatttttg
     1201 agaaacaacg tacaaaataa tcgcagcaag caatccaatg gtgcccaaaa ctactaatga
     1261 aagaaaaatt gtattcattt tttatctatt ttttcaacat aaaacataaa ctgatccttc
     1321 agtttattct gcaatagaaa tagtattaaa taatatggta ttagcacaaa taatgtacta
     1381 attaccattg ttaaatcatc ttttttgtaa tactccgcaa caaacaaaat aagcaatatt
     1441 agaaaaacag gaataacaaa agccatgaga acagctttta acccaaagtt ttttttgcct
     1501 attatacgta ctgtttcgcc aatctcaaaa ctctcattat taatagttgc ttctattaat
     1561 ttagagctaa catcagcaga actacaagca cctttcgttt gacaagaaac acaagcagca
     1621 agttgtttta tgcagactgt tactgtttct tggtttattt ttacgatggt tccagtatat
     1681 tcctgagaag aattcatacg taaatcattt tttgaaagca caaagttact gtttatttta
     1741 tatattatac gtatttttta gttaattttt cattttattt taccaaaaag agtacctttg
     1801 caagcatata aaattttaac taaatgcaac aaggagaacg atacaaagaa attagaaatg
     1861 taacgcttat tggttttgta gttaatctat tgcttaccct tgccaaactt tttgcaggta
     1921 ttgtaggtaa aagttctgct atgattgctg atggggtgca ttctttgtct gattttgcga
     1981 cagacattgt ggttattgtt tttatgagag tatcagacaa agaaagtgat gagaatcata
     2041 aatatggtca tggtaaattc gaaacctttg ctattttaat aataagtatc tgcttatttg
     2101 tcgttggaat agggttgtta ataggtggta ttgataagat tgtttatttt tataagggga
     2161 atgaaataat gcaaccgagt ctgttggctt tttatgcggc tcttgtttcg attgctttaa
     2221 aagaagcgtt gttttggtat acatttaaga ttggtaaaaa agtgaatagt gatgctgtta
     2281 ttgcaaatgc atggcatcat cgttcagatg cattttcttc tatagcaaca gccttaggta
     2341 ttgcaggagc aatattttta ggagaaaaat ttagaatctt ggatccaatt gctagtgtac
     2401 tagtgagttt ctttattatg aaagtcagtt atgaattagc aatgcctagt gtaaaagaac
     2461 tgttggagtc ttctttacca aaagaaaaag aagatgaaat aattgaccac gtattgtcta
     2521 taccaggtgt aattacatat catcatttgc gtactagaaa agtaggtaat ctcattgcaa
     2581 ttgatattca tattaaactg aacaaacata taagttttgt agagtctcat gatattgcta
     2641 cagacgtaga atctttgttg cgtcatcaat atggtaatca aacagttgtg aatatacata
     2701 cagaacctta tgaagaaaaa aaataaaggt ttaatcatgt gcattatttt ttatatttga
     2761 ttttatttag aaagatacag ttgttttttg acattttttt taatattttt cttttaaata
     2821 gtttgtttga ataaaaaaaa gacagtatct ttgccaaaca aaacaacgat tatgtcattt
     2881 agcactctac atactatttt acttagtatt cttctctgta aaggatgagc ggagtgagtt
     2941 tggtatgtgg aaaattaata ataacataga aagcctttct tacttcggta atgagaggct
     3001 ttttcttgta atttttgtaa gaacaatata aatacgtgag gatatggaca gattatttat
     3061 ttttgacacc accttgagag atggagaaca agtgccaggg tgtcagttga atacagtaga
     3121 aaaaattcag gtagcaaaag ccttagaatc tttaggtgta gatgtaattg aagcaggatt
     3181 tccagtatct agcccaggag actttaaatc agtggtagaa atatccaaag ctgtaacgtg
     3241 gcccacaatt tgtgcactta cacgtggtgt acaaaaagac attgaagtag ctgctgaagc
     3301 tttacaatac gctaaaaata aacgtattca tacaggtatt ggtacatcag attatcatat
     3361 aaaacacaaa tttaattcta cacgcgaaga aatattagaa cgtgctgttg ctgctgtaaa
     3421 atatgccaag aagtatgtgg aagatgtgga attttattgc gaagatgctg gtcgtagtga
     3481 taacgaatac ttagcaagag ttgtagaggc tgtaatcaaa gctggagcaa ctgtggttaa
     3541 cattcctgat acaacaggct attgtttgcc atcacaatac ggagagaaaa ttaaattttt
     3601 gatggaaaat gtaaaaggag ttcataatgc tatcctttct actcattgcc acaacgactt
     3661 agggatggct actgctaata caatagctgg ggtattaaac ggagcacgtc aggtagaagt
     3721 aaccatgaat ggtattggcg aaagagctgg taacacctca ttagaagaaa tagcaatgat
     3781 tttgaaatgc cacaaacatt tagaaataga tacccaaatt aacacacaaa aaattgcaag
     3841 cactagtcgt ttggtgtcta gtttaatgaa tatgcccata caacccaaca aagcgattgt
     3901 gggtcgtaat gcctttgcac attcatcagg tattcaccaa gatggtgtgt tgaagaacag
     3961 agagagctat gagattatgg atcctaaaga tgtgggaatt gatgaaaatg caattgtttt
     4021 aactgctcgt agtggtcgtg ctgctcttaa acatcgttta agtatattgg gtgttgctgt
     4081 agaaggcgat gccttggaca aaatttacca agacttttta gtgcttgccg ataagaaaaa
     4141 agacgtgaac gatgatgacg tattgatgtt ggctggtatg gaacgcacaa gcaatagccg
     4201 tattaaactt gattatttac aagtaacatc aggaaaaggt gtacgtgctg tagcaagtgt
     4261 gggattagat attgctggcg aaaaatttga agcagcagct tcgggtaatg gtccagtaga
     4321 tgcagctatc aaagcgttaa aacagattat taaacgcgaa atgtcattac aagagtttac
     4381 tattcaggct attaataaag gaagtgatga tataggtaag gtacacatgc aagtacaata
     4441 caaaggtagc gtgtattatg gttttggtgc caatactgat attgtggcag cctctttaga
     4501 agcatacatt gatgcaatta ataagtttgt gaaataaaaa ataacataca tacattattc
     4561 atttataata ctaacctaca taatagttta actcatgaaa aagactttat ttgagaaaat
     4621 atgggacgca catgtcattt ctgaagtaaa tgatggtcct actcaattgt atatcgatcg
     4681 catgtattgt cacgaagtaa ctagtcctca ggcatttgat ggaattagaa aaaggggtct
     4741 aacggtattt cgcccaaagc aagtaacatg tattcctgat cataatatcc ctactatcaa
     4801 tcaggaaaaa cctattgaag atccaatttc aagacaccaa gtacaagcct tggatagtaa
     4861 tgcaaaagaa tataacttgc agtattttcc gatggggcac aataaaaatg gtattattca
     4921 cgtagtagga ccagaaaatg gtttatcatt gcctggaatg acaatggtat gtggcgattc
     4981 gcacacatct actcacggag ctttaggggc tgttgctttt ggtataggta ctagtgaggt
     5041 agaaatggta ttggcgacac aatgtatttt tcagtcaaaa cctaaaacca tgcgtattag
     5101 cgtgaatgga aacttaaaag atggtgtttc agctaaagat attgctttat atattattgc
     5161 taaaatgaca actggtggtg ctactgggta ttttgtagaa tatgcaggta gtgccgtaca
     5221 agcactttca atggaaggtc gtttaacctt gtgtaatctt tcaatcgaaa tgggagctcg
     5281 tggaggtatg gttgctccag atgaaactac tttcgaatac atcaaaggtc gtgaatttgc
     5341 tccaaaaggc gaagaatggg ataaagcctt agcgtattgg aaatcattac caactgacga
     5401 aggggctcaa tttgataaag atattgcatt cgatgcagct gatattgaac caatgattac
     5461 ctatggaaca aatccaggaa tggggttagg aattaccgaa tcaattcctt cattggatag
     5521 tattgatgag gcaggtcgca tttcttttgt taaatcattg gattatatgg gctttaaacc
     5581 tggagataaa atgataggta aacccattga ttatgtattt cttggaagct gtacgaatgg
     5641 acgtattgaa gattttagag catttgcttc gctagttaaa ggaaagaaaa aagctgataa
     5701 tgttgtagta tggttagttc ctggttcgtg ggctgttgaa aagcaaataa aagaagaggg
     5761 tattgatacc attttaaccg aagcaggttt tgtacttcgt caacctggtt gttcggcatg
     5821 tttggcgatg aacgatgata aagtccctgc tggtaaatat gctgtttcga cttcaaacag
     5881 aaattttgaa ggtcgtcaag gacctggagc acgcacaatt ttggcaggtc cgttagttgc
     5941 tgcagcagct gcgataacgg gtaaaattac cgatccaaga acgttgtaga gagtaaatga
     6001 tttttagtat taatagtata gtaatataga tagaatatgg aaaagtttaa aacaatcagt
     6061 agtacagtag tgcctttgcc tatcgaaaat gtggatactg atcaaattat accagcacgg
     6121 tttttaaaag ctacttccaa attaggcttt ggagataatc tttttcgtga ttggagatac
     6181 gataaaaacg accaaccaaa tcctgatttt gtgcttaata atccaaccta tagtggcgaa
     6241 atattggtag caggtaaaaa ttttggtagt ggttcaagtc gcgaacatgc tgcatgggct
     6301 attgcaggat acggatttaa gattgtaata tcaagttttt ttgcggatat ttttaaaaat
     6361 aatgccatga acaatggggt attgccagta gtggtttctg aagcattttt agccgaaata
     6421 ttctcttctg ttgaaaataa ccctaagacg attattacag tagatcttca aaaccaaaca
     6481 gtaactaaca ctgctacaaa taaatccgaa aaatttgata taaattcgta taaaaaagag
     6541 tgttttttgc aaggatttga tgatattgat tatttattag ctaaaaagca aataattgaa
     6601 caatacgaag ataagcaatc tgatgtagct tattttctta caacttaatt gacacaaaga
     6661 atgataaaaa ttttagatac aacattgcgt gatggagagc aaacctcagg ggtttcgttt
     6721 gctatacaag aaaagctgag tatcgcacgc ttattgctag aagatttaca aattgataga
     6781 attgaagtgg cttctgcacg cgtgtcggat ggcgaatttg cttctgttca acaaattacc
     6841 aaatgggcaa aaagcaaaaa tgtattggat aaggttgaaa ttcttggttt tgtcgatggt
     6901 tttacatccc ttaaatggat agaaaaagcg ggagggagag taattaattt actgtgtaaa
     6961 ggctcgttaa atcattgtac tcatcagcta agaaaaacac cccaagagca catagctgac
     7021 attaagcaag tggttgccaa cgcacaatca atgggtatta cagtaaatat atacctagaa
     7081 gattggtcga atggcataca gcaaagttct gaatatgttt ttaccttaat ggacagtcta
     7141 aaagatgaac caattgtaca tttcatgtta ccagatacac tggggattct aaatccaaac
     7201 caaactcgca cgtattgttc aatgatggtt gaacgatatc cttcgttgca gtttgatttt
     7261 catgctcata acgattacga tatggctgtt gcaaatgtat ttgaggcagt acaagtaggt
     7321 attcaaggtc ttcacgttac tatcaatgga ttaggtgaac gtgcaggtaa tgctcctctt
     7381 gctagtgtcg tagcaatgct acatgaccaa cttcacttat cgaccaatat taatgagcca
     7441 gaattaaacc gtgtaagtaa gcttgtagaa gcatattcgg gtatccgtat tccaccaaac
     7501 aaacctgttg tgggcgataa tgtgtttaca caatgtgctg gtattcacgc tgatggtgat
     7561 gcaaaaaaga atttatattt taatgattta ttgcccgaac gctttggtcg cattagagaa
     7621 tacgctcttg gtaaaacatc tggaaaagcc aacatcagaa aaaatcttga ggctttaggt
     7681 attgagttgg atgaagagtc catgcataaa gtaactcagc gcattactga gttgggcgac
     7741 caaaaacaag tggtaacaca agacgatttg ccatatattg tttcggatat tgtaaaagaa
     7801 gaaaatattg ctgaaaatgt acgtatcctt aattattctc tatcactggc tcagggatta
     7861 aagccaatag ctaatatttt gatagaaata aatggagaaa catatcaaga atatgcaact
     7921 ggcgatggtc aatacgatgc atttatgaag gcattatgga aaatttacga tacattagga
     7981 aaacctcatc ccattttgct ggattatttg gtaactattc ctcctggagg tcgcacagat
     8041 gctttggtac aaacgttaat aacttgggat ttggatggaa atgtgtttaa aacacatgga
     8101 ctagatggcg accaaacggc tgctgctatt aaggctacac tgaaaatgct aaatcgtata
     8161 taattacttc aaatgaatgg agtaaattga ataaacagta ataaaatgaa taaacaacta
     8221 accattgctt tggtagctca cgacagaaga aaggcagata tggtagaatg ggcgttgcat
     8281 aatgcccaaa ctttatctca acataagtta gtgtgtacag gtactactgg aggtttaata
     8341 caagaagctt ttgaaaataa atcaattgct gctgatatta cacgagttaa ttcaggtcca
     8401 atgggtggtg atgcagagat tgctgctatg gtcgttaaac accaaataga tttagctata
     8461 tttttgattg atgacttaaa tccacaacct cacgaagctg atattcaaat gcttttgcga
     8521 caatgtaggg tgcataatgt gcctattgct tgtaatagat acagtgctga tttgatgata
     8581 actagtacgt tgtgggataa tcctacttat cagccaactg aaccaaaata tgttgtattt
     8641 gaacggaaat agtacaacat tagcaaaaaa taatccccaa ttatctctat taagtatact
     8701 tttaacataa acaataacta ctaaaatgaa attaaacatt gcagttttgc ctggagatgg
     8761 cataggacca gaaattatta aacaagggtt agatgtaacc attgctgttt gtgaaaaatt
     8821 tggacacgaa ttaacctatg agtatgggtt agtgggagct actgccattg accaagttgg
     8881 taatccttat cctgatgcta cacatgaatt atgtatgaaa tccaatgctg tactttttgg
     8941 agcagtagga gatcctcgtt ttgataacaa cccaagtgct aaagtacgtc ccgaacaagg
     9001 cttactagct atgcgtaaaa aacttggttt atatggaaat attcgtcctg ttacaacgtt
     9061 tccgtcgttg attcacaaat ctccattacg tgcggattta attgatggtg ccgattttgt
     9121 atgtatcaga gaattaacag gtggtatgta ttttggtcgt cctcaaggta gaagtgaaga
     9181 tggaacagta gcttatgata cctgcgttta tagtcgtatg gaaattgaac gtgtgttgac
     9241 gttggcatat agctatgcgt taaaacgtag gaaaaaattg actgttgtag ataaagcaaa
     9301 tgtattggca acttcgcgtt tgtggagaga agtagctaaa gaaatggcac aaaattaccc
     9361 tgatgtagaa acagaattta tgtttgtgga taatgcggct atgcgtatga ttcagtggcc
     9421 caaagatttt gatgtgatgg taaccgaaaa tatgtttggt gatattttaa ctgacgaagg
     9481 ttccgttata actggatcta tgggaatgtt gccttcagcc tctataggcg aacatacttc
     9541 tgtatttgaa ccaattcatg gatcgtggcc ccaagctgct ggaaaagaca ttgcaaatcc
     9601 attagcaact attttatctg ctgccatgat gtttgaatat gcttttgact taaaagaaga
     9661 agctgcttta attcgcaaag ctgtagacgc ctcaatggat gctaatgtac gtaccgctga
     9721 tattcaagta gaaggaaaag aagggtacaa atgcagtgaa gtaggagagt ggattaaaaa
     9781 ctggattaaa aataactaag ttacatttat atttgctagt taaacataaa aaaacgaaca
     9841 tcataatcga ggttcgtttt ttttatgttt atatgtccca ttttaccaag ttttacgctt
     9901 gctataacga tcggattttt tgtttttatc tgtttcttta ttcctatctc tgcctcttcc
     9961 tgacgattga ctgcgtgaag cagatgatga agattgatta gcaggagctt gtcttctgcg
    10021 tgatggagaa gaagaggaag agcgacttga cctatctcct tttacctcat ttaattcgat
    10081 actacgacta aatttgctag tagcaaatgc tgtttttaaa cgagtaactt ggtctttatc
    10141 tacatcaaaa aatgtaaatt tagatgtaac ttcaatatct ccaatattaa tatctttact
    10201 atcagttacc tcattaatga gttttaataa tcgttttggt tcatacccat cgtttttacc
    10261 ttggtctatt tttaaccgaa ttctatccga tttccctgtt ctacgattgc gtgcctggcc
    10321 tccatctttt tctgccttaa tatcgttatt caaatcttta gaatctttgt aataacttaa
    10381 aaagcgatta aattctaacg aaacaaaacg agtaatcact tcttctttgc tcatatctgt
    10441 tagcatctca aaaatctcgc tcaagtagtt atcatctaag tcctcgctaa cctccacttc
    10501 acgcatacgg tgtataaggt ggtttaattg tttttggcat gcctctaatc ctgtaggaat
    10561 gagttcttgt ttaaattttc tatttattgc tttttcaatt gtgctaagag aacccttttc
    10621 tctaggagtt acaatagcaa tagaaattcc tgatttattg acacgccctg tacgtccgct
    10681 tctatgggta tacgtttctt tatcatcagg caaatcatag ttaataacat gtgtcaaatt
    10741 gtttacatct aatccccgtg cagcaacatc tgtagctact aaaatctgta aatttttaat
    10801 acggaattta ttcattacca tgtcacgttg tgcttgcgac aaatcgccat gcaaggcatc
    10861 agcattatac ccatcgacta taagtagatc tgcaatatct ttggtttgtt ggcgagttct
    10921 acaaaatata atgccataaa tatctgggtt gctatccaaa attcgtttta gagctaaata
    10981 tttttggtct ttacgtacta ccacacatac atgctcaaca tttttggatg cctcattttt
    11041 tttaccaaca gcaatttctt ttgcattact catataacga cgagcaatac tgcttatctc
    11101 tgtgggcata gtagccgaaa ataataatgt ttgtcggcta tcaggagttt cttcgatgat
    11161 ggttgttata tcatctaaaa atcccatttt tagcatctca tcagcttcgt ctaatattaa
    11221 atgtttaatt ccttgcaacg aaacttttct acgattaatc aaatcaatta atctacccgg
    11281 agtagctact acaatatgcg gtgttttatc gagttgtttt aattgtttca taatgtcttc
    11341 accaccatat accgctacga tatgtatacc ttctttgtag gtactatatt tacgtaattc
    11401 tgtggctatt tgcatgcata attcacgtgt tggagctaag actaaacctt gtgttgttct
    11461 ttcttttgtg ctgattaact gaattagagg caaaccaaaa gctgctgttt ttcctgttcc
    11521 tgtttgtgct aaaccaatga tatcagattg attagaaagc atgtaaggga taacttcttc
    11581 ttgtatgggt gttggagaaa caaatcccat ctcactaatg ccctgaagaa ttgattgttc
    11641 aagacctaat ttttcaaatg tattcatgta aaataataaa tgatgaggaa aataaatagc
    11701 gaaaaaacaa agagggatgc gtattaaaaa cggcaaaatc agtccgtgtc atttagtaaa
    11761 cctatttttt cactatataa aagatgttgt atataaaata aataattatt gcggtacaaa
    11821 ggtagttttt tatttttgaa taataatgat attcatactt ttttgtttaa aaatagagtc
    11881 tttactagtc gtttgaaaaa aaaatactgt acctttgtgg gcaaaaattt atgaagaaac
    11941 tactacatga aaccaaaaac taaaaaaacg attcttattg gactttggac actttttgcg
    12001 tcaatcatag ttggtacagt attgctattt ttccttatag caaaaggtgt tattggttat
    12061 ataccagaaa ttgaagagtt gcaaaatcca aaaaataaat ttgcatctga aatatacacg
    12121 gcagatggag aggttttagg aagatttttc tatggtaaag acaaccgagt agcggtaaat
    12181 tatacccaaa tatctcctta catgatagaa gcattggtag ctacagaaga tattcgtttt
    12241 tttaaccatt ctggtattga tgcacgttct ttgggaagag ctattatttt acgaggttta
    12301 cttggaaaag ccagtgcggg tgggggtagt actattacac aacagttagc taaattactt
    12361 tattctccta atacagataa tctctttcag cgtgctatgc aaaaacctat agaatgggta
    12421 atagctgtac aactagaacg tttttatgct aaagaggaaa taattgcaat gtatcttaac
    12481 caatttgatt ttttgaataa tgccgttggt ataaaatcag cttcacaggt atatttcaat
    12541 gttgcaccca atgaactaac tagagaacaa gcagctttgc ttgtaggaat gtgtaaaaac
    12601 ccttcgttat ttaatccaaa ccgctttcca gaacgtgcta aaaataggcg taatacagta
    12661 cttgaccaat tgtataagta tgattatatt actaaacaag aagtagattc attaaaatca
    12721 attccaatta aattgaattt ccaacgagtt gatcataagt taggtttagc tccttatttt
    12781 agagaatatt tgcgtcaaac gatgatggct gataaacccc aaagaagcaa ttatatgtcg
    12841 tggcaaatgc aaaaatataa agaagactcg ttagcctggg aaacaaatcc attgtatggt
    12901 tggtgtaaaa aaaataaaaa accaaatggg tcaaattaca atatttatgc agatggatta
    12961 cgcatttata ctacgataga tgcacgcatg caacggtatg cagaagaggc agtgaaagag
    13021 catattggag ataatttgca aaaacgtttt tttaacgaaa aacgtggacg ttcgtatgct
    13081 ccattttctc gaaatattac tccagaagaa atgtctagca ttttaacaag agcaaaacgt
    13141 cagtcagata ggtatcgcaa tatgaaaaaa gccaattata caaatgctga aattgatcgt
    13201 gcttttgcta aaaaaatacc gatgagtgta tttacttgga caggtgttcg tgatactatt
    13261 atgagcccga tggattctat caaatggcaa aaacactttt tacgctgtgg atttatgtct
    13321 atggatccct ataacggaca tgtaaaagcc tatgtaggtg gaccaaactt tgctgaattc
    13381 caatatgata tggtaacgca aggaaaacgg caagtgggtt ctaccatcaa gccttattta
    13441 tatacactgg ctatggaaga agggttgact ccttgtcaga aagtgcccaa tattccgcaa
    13501 accttttatc tagaggatgg aaaagagtgg acaccacgta atgcaagcaa ctcgaaagag
    13561 ggagaattag ttacgttacg ttggggattg gcaaattcaa acaactggat tacagcatat
    13621 ttaatgaaac aaattacccc acaagcattg gttaggatga tacattcttt tggtattcaa
    13681 agtcatctgg agccggttgt ttcattgtgt ttaggacctg ctgatgtgtc tgttaaagag
    13741 atggtgggag cctattcttc ttttgtaaac aaaggtatac gtgttgaacc gctgtttgtt
    13801 acacgtatag aagataataa aggaaatgtg ttagcaaatt ttactcccca attggtagaa
    13861 atatttagcg aagaaacttc ctataagatg ctgacactct taggagccgt tgtaaatgag
    13921 ggtactggtg ttcgcctacg ttatcgtttt cagcttagag gggaaatagc agctaaaaca
    13981 ggtacaacgc aaaataattc agacggttgg tttattgcta caactcctca attagtcgcc
    14041 ggagtatggg ttggtggaga agacagatcc attcactttg acaatatagc agatggtcaa
    14101 ggggctaata cagccttacc tatttgggca atttttatga aaaaagtgta tgcagatact
    14161 agtttgcctt attcagaaaa tgttaaattt gatataccta cagatattcg acaaggttat
    14221 gactgtcgtt ctgaaccaaa tacagaagaa agttttgatc aaatattcga tttagaataa
    14281 cgaaataaac cagactattt gtctatatga aaatttgttg tacctttgaa acatacattt
    14341 aaaacgtaag attaacccta aaaatagaaa agaaacatga aaattcattt caaattaaat
    14401 tactttacaa aatgggggca gaatattgcc ataatggggt ctatcccaga attatcgaac
    14461 aatgatcctt ctaaggcatt gtatatgaat tttgaatgga aagaggattg gtcgttggat
    14521 attgacgtgc aacgtgatgc tccatttgaa ttaacataca aatacgtatt aaaagacacc
    14581 aatggattgg atgtgttgga gtggggtgat gaccgaacaa ttttagtcga tccattaaga
    14641 gatgaaaata tatattgcta tgatagttgg aatcctgctg gtgctgtaga aaatgtgttt
    14701 atgacctcac cctttcaaaa tgtattattc aaagaaaatc atatccctgt acctgccatt
    14761 acacctaaaa aatacacaca tatttttagg gtaaaagcgc caaccttgaa aaaaggggaa
    14821 gcgttgtgtg tggtaggaag cactaaagaa ttaggtgatt gggcaagtga gaaaccagtt
    14881 gtgatgagca atgaagacaa aaattggtgg gtagcaaaag taaatttagc aacaaccaag
    14941 gtcgatgttg tgaattataa atatggagta tatgatattg aagaccagtc tcttaaatat
    15001 tttgaatacg gtgcagatag aaaagctacc gtagtaacaa caaagaaatc gctcgttatc
    15061 gtttctgatt cttttgcacg tattggatca tacgatttta aaggtgctgg tgtaggtatt
    15121 cctgttttta gtatccgtac aaaatcaagt tttggggtag gtgattttat cgacatcaaa
    15181 ttaatggttg actgggcaaa aaaagtaggt ctgaaactta ttcaggtttt acccttaaat
    15241 gacaccattg gtacacatac cgcagccgat gtattgcctt atgctgctat ctctgctttt
    15301 gccttaagtc ctttgtattt aaacctacct aaaatgggta agttgccagc agacaatccg
    15361 ttaaataaag catacaaaac caaacaaaaa gcgttaaatg atttgccttt ggtagagttt
    15421 ctagaaattg taaattttaa attagcctat tcaaaagcat tatacctaaa aaataaagag
    15481 aaatttctga aaaataaatc gtacttaaaa ttcttcaaag aaaacaaaca ttggttggtt
    15541 tcttacgctg ctttttgcta tttacgtgat aaaaacggta ccgcagacca ctcaaaatgg
    15601 ggcgaatatg caacattttc tgaggcaaaa ttggaaagat taacatctcc agaacaagct
    15661 cattttgatg atattgcagt aaattatttt atacaatacc atttgcattt acagttatta
    15721 gaagcagctg aatatgctca tgaaaacgga gtaattctga aaggagatat tcctattggt
    15781 gtaaacagaa acagtgttga tacatgggtt gctcccgaat tatataatat gcatatgcaa
    15841 gctggagctc cacccgatat gtttgctatc aaaggtcaaa actgggaact tcctacctat
    15901 aattgggaaa aaatggaaga aacaggattt gattggtgga aaaaacgttt tcaacaaatg
    15961 gggtattatt ttgacacctt ccgtatcgac catatcttag gatttttccg tatctggcaa
    16021 attcctttgc accaagaaga aggtattatg gggtatttaa acccatcaat tccagtacat
    16081 attaatgagt ttggcgaaaa aggtgtacat tttgattaca atagattctg tgtgccattt
    16141 attaccgatg cagtattgtg ggaagtattt ggtgatgatg ccaactgggt gaaaacaaat
    16201 tgcatcgaca tcattgatgg ttggatttta cgccttaaac caatatgcaa tacccaaaag
    16261 aaagtaatgg aaatgtttga taaaaacatg attactgaaa aaattaagtg gggcttattt
    16321 gatttaattt caaatgtatt gttttttgaa gttgaatggt cgaatggaat gatgtattac
    16381 ccacgttatg gtatgcaaac cactactagt ttccgttatt tagataattt tacgaaaaat
    16441 aaaattgaag aactgtatgt agattatttc tatcgtcgtc aagatagttt ttggaaatat
    16501 tcagcattaa aaaaactacc agctatcaaa cgttctacca atatgatgat ttgtggcgaa
    16561 gacttaggta tgatggcaga ttgtgtaact agcgttatga atgaattagg tattttaagt
    16621 ttagaaatac aacgtgctcc taaagttgac accatcgaat tcttccatcc tgccgatgct
    16681 aaatatttat cagtagttac accttctacc cacgatatga gcaccatcag aggctggtgg
    16741 gaagaggata gggaagttac ccaacgattt tacaaccaac aacttggtca ttggggtgaa
    16801 gctccttact ttgccgaatg gtgggtatgt agagatatga ttgttcagca cttatattct
    16861 ccagctatgt gggcaatatt ccagttacaa gatttattaa gtatttcaga tacattaaga
    16921 cgtcaaaatc cacatgaaga gcgaataaat gtgccatcaa actccaaaca tagttggaga
    16981 tacagaatgc accttaccgt agaagaatta atcgaacaag aaacctttaa taaagagctg
    17041 aaaaactaca tagtacaagc taatcgttaa ttattaaaaa aaatgtccaa gaatttcttg
    17101 gacatttttt tataagtagt tgacagaaat aaacgctcaa cgctcaacct ttcccctaaa
    17161 taaagggacg tattgaaacc aaaggatata tcctaagaac aataagaaca ctacaatata
    17221 aaagaagtag ttagataaat catgggcatc catagctaat tgatagcttt gatgcttata
    17281 caaatgtaaa agtaaaaata cgatacggac actattcata ataatcatca caaacattcc
    17341 aaatacaata tatgtaagtt tatttatcaa agaactgcga atgagtaaaa tgaggagtga
    17401 aaagcttgtc ataataccaa tacccaaaca ttgagctcca actcccacat aattaattcc
    17461 caaataaaga tagttattaa caataattgt ttcgctgtaa ccaatcaaaa acataaattt
    17521 ctgtgacaac cataaaatta tatttagcaa ccaattaaaa gtgccataag gtgcaattaa
    17581 atcaagaatt ttatgaaaat tcacaaacat aggaataatt gttatcattc ctattaatga
    17641 taaactgaat tttaattgta ttttatcatt taatgattta atatagtttg aggaaaattc
    17701 aaacaattga aattttaata aaagcagaac aactagtaag gttttaatat acaagtttgt
    17761 tgggtcactc aattgatagg ctaattttcc atcaaacgtt aaaagcatag caagtttgaa
    17821 caaagaaagc aaaaagagga ataagatacc acaaacgata tataaaagac tcatattttt
    17881 gtttatgaaa aagaaagaaa agagaaacaa tataaaaaga taatatttta gggcaacaaa
    17941 aggctgaata gaaaatgtct cttttccaac aattccaata tactggatta aggcattata
    18001 cgacacatta aatccaaata aacgtaaaag agaagaactt gtttgtccta aaatataagc
    18061 aaaaaaggat tcgatttgct tatacaacaa agagttttct acccaaatat taccaccaaa
    18121 aagaaaaaat agtagcaatg aagctccaat tactatagaa aaaggataat tcttgaataa
    18181 tttttggaat aaaaagggta attcttttct tttcataaag ttgaattgtt aattgtaatt
    18241 tgagaaagca aaagtattta tttttttata tttataatct atttacgaat aaattgagga
    18301 ttttaattga ttttctgctt ctgtaaacta gatagaaata aataaaccta tctttttaat
    18361 attaattttt tgtgtaagtc ttctaaaaca actacatttg tagcggttta taatcaaatt
    18421 ttgtattgat gcgaacatta gtcttattta taataagttt gttgttgttt ttttcttgtg
    18481 ttacaccaag aaaaacgcat tattttcaag aacctgatga cgttattcca tcatactctg
    18541 aagctatttt tccttctgat tatcaaatcc agtttaatga tgaattatat atacgcgttt
    18601 tgacattaaa tcctgattct aaacgtattt ttaaccctca attatcaaca acaagttcat
    18661 ctagttcagg aaacgttaca acctctccta taaaaggcgt tacatcctac accgtttatg
    18721 atgatggaac tattgatttc ccgtatcttg gtgctgtatc tgttgcaaat aaaactaccc
    18781 gtgaaatcaa agttttattg gaagaaaaac taagtgagta tgtaaaagac tgttcagttg
    18841 atgttcgatt agtaaacaac tatgtgaatg ttttaacaaa tacaggtgca tctagagttc
    18901 aattagtaaa agagcatacc aatatatttg aggtcttggc aatgtcgcat ggattaagtt
    18961 cgtatgcgaa acgatctgat gtgaaaattg tgagagaaac tgcacaagga tctacaattt
    19021 attcttttga tttgagaagt aaagatatta tccattctga tttttattat gttaaaccga
    19081 atgatgtgat atatgttcct gatttcgatg gtcaattttt tcgcttaaat aactttatga
    19141 cagtattaac aacaacaaca acaacgatat catttgggtt tttcgtttgg aaattaatag
    19201 atttgtggat accaaaacaa taacatataa gcatttagtg atactaatac ttgtcttttt
    19261 tagtatggct tcatgtatta caaacaagtc aaccgcttat ttgcaagaag ggggaaatat
    19321 gccccaatac gaagaaaccg aatacacgta ttaccgcata caaccaaacg atgagctagc
    19381 catgcaagta ttaagcatta acgaagaggt agttgctttt tttaaagatg gacaagcagg
    19441 tgctactcct atttcttaca aagtatatgc tgatggaacc attgatattc cgtttatctc
    19501 tggtattcat gtagcagggt tgacggtaag agaagctgca aaaaaaattg agctacaagt
    19561 tcgtgaattt gcaccagatg ctaccgtaaa gttaggttta tcaaatgact ctttttatct
    19621 cataactgaa gggaaacaag gcaaatttcc catgtacaaa gaaaaactaa acttgtttca
    19681 agccttatca ttagccggaa atatacctat aaatgccgac agacagcgtg tgaggataat
    19741 tcgacaaaaa gatgccatgt ctccacccat catcaaggaa tttgatattc gtacagaaag
    19801 catcatccat tccgaatttt attacattca gccaaacgat ctaatttatg tttctacaat
    19861 aaaaggagat ttttttaaag taaccgatta ttcatctaca attaccagta ttaccagtac
    19921 gttgtctttt ttactgctaa tcatgggatt attttaatat atttttatgg aaaacaacgt
    19981 acctcaaaat aattttaatg aagaaaacga aagttcttct tttgatatta aaatatggat
    20041 tattcggatt ttaaaaaact ggtatctttt tttcatatct atcagtatgt ttcttactgt
    20101 agcttatttt caaaataaaa gctggtttcc tatttatcgt tcgtcagctt tggttattat
    20161 tgaagaagca aatagaggtt tcaacaggag ccaacaagct tttatggaag gattcagtgt
    20221 acaaggagga tatagaaatg taaacaatca aattgtaatg tttagttctt tggacttaat
    20281 caaaaaggta gtagataatt tacaagattt acatatagaa tactatacac gaggacgttt
    20341 tacttcaaca aatttataca aaaaatctcc tgttttcatt gaaaagttat ttatatcgcc
    20401 taatggatat gaacaagaat tctttttaga ggatgtagga aatggaaaag attttttaat
    20461 aacatatgct ggcaataaac aaattgcaga atttacagtt aaaggagaat atggaaaaca
    20521 gctagaaacg tctcttttct ttattactat tcataagtct gatttttatc gtaaccgctt
    20581 tcaattgtat tttggtttta gggatttaga taaattagct tctatgctac atgatcgctt
    20641 aacatttgcc tttaagatgg agggctcttc agtattagaa gtatctatta ctggcaatgt
    20701 gcatgaacgg gattgtgatt ttctaaatgc tttgtgtgat gcgtttttag cagaaggttt
    20761 agctcgaaaa aatgatgcag caataaaaac aattgagttt attgattctc agatgcttac
    20821 gatatcagat tcgttgcgtg tctcagaaag agcattgcaa aactttaaat cagataattt
    20881 tatggtagca gctaattcaa gtactgaatt gtctgccaaa tatcgagctt tagaaacaaa
    20941 agaaacagaa ttacgtttaa aggactccta tattaaatac ttaacgaatt atcttgaaaa
    21001 gaacattgag gatggatccc tgattgctcc tacaagttta ggggtaaatg aacctacatt
    21061 aacattacta gttaaagatt ttactgaagt aatgttcaag aagaaagagg taggagaaag
    21121 aagtcccttg tatgctaagt atactcgtga tttatcgaat ataaaagagc agatgtttga
    21181 agcattacgc aatatacgtg ttggttttaa tattgaaaaa gctgattttg acaaacgaat
    21241 ggctgagttg aaggctcaaa tacaagtatt accaataaaa gaacaacaat tactcaattt
    21301 tgagcgtaag tttaaggtaa acgataatta ttattcgttt ttattacaaa aaagagcaga
    21361 tgcacaaata ctaaaagcat caaattcacc tgataatatt attttggata aggctcgctt
    21421 gacaggtgtt acaaatggtg ccagtaaagg gagtaattat agtacttttt tattaatcgg
    21481 acttctaatt ccattagcat ttgttgggct aaaagaattt atgtatccct ttattcgttc
    21541 tgaaaatgag attttgcatc tttctcgcag ggcataccaa attattgggg ttattcgtca
    21601 tacctcaaaa aacatacctg taattgtaga tgcctatcca cgttctgcta tggcagagat
    21661 atttagagta gtgcgtacac gtatggaatt tttaaatggg aagaaaaaaa acatgactat
    21721 gatgtttact tcaactcatt caggagaagg taaaacatat atatctgtaa atatggctgc
    21781 aatatatgct atgacaggca aaaaaactat cttgattgat tccgatttgc gtaaacctag
    21841 tgtttctgct actttatcat tgccagaaaa taaggggttg agtaattatt tagcgggaca
    21901 gattgatttg tcagaggcta ttataaaaaa atcaaattac aagtttgata ttatgacggt
    21961 aggagtcatt cctccaaatc caggagagtt aatgaaatct caaaaaatga aagaattaat
    22021 caaagaatta aaagaaatat atgattttat tatatttgat gctcctccta ttggtttagt
    22081 agcagactct tatgctatta taaatgaaat ggatttaaac atttatgtag tgcgttgtga
    22141 gcgaaccaat aagcccttat ttaaaaagac aattacgcaa ttgcaagaag ataaaatacc
    22201 gaatgtccat gttttattta atgatataaa tataaaaaaa ttagaatacg ctacttcata
    22261 tagtaactat tctaaatacg gcaagtctta ctataccaat ttattataca aaaactacaa
    22321 agataataac ggcgtctatt ttgatgattt agaagatgat gtttaattga cacttatcac
    22381 taaacattga cactaatcac taatcattat taacatgaat gtacaagaca aaaaaattgc
    22441 agtaataggc ttagggtacg ttggacttcc cctagcaatt gaatttggta aaaaataccg
    22501 tgttcttgga tttgatatta aagcagcacg gattgaagag ttacaagctg gagaagattc
    22561 tactttggaa gctgatttgg aaggtatgaa aatagccact gatttgtata aaaaaaatga
    22621 acaaattggc ttgtcttttt catctattca agaagattta aaaaatttta acacctttat
    22681 tgtaaccgta ccaactccca ttgatgaatt taataatcca gtgttaacac cccttatttc
    22741 agcatctcgt atgattggta atgtgttaaa aaagggagat ttagtgatct atgaatcaac
    22801 agtatatcct ggttgtacag aagaagattg tgtacctgtt ttagaaaaac aatcaggttt
    22861 aaaattcaat aaggatttct tttgtggcta ttcgcccgaa cgtatcaatc cgggtgataa
    22921 agtaaacaca ttaaccaaaa ttagaaaaat aacatcgggt tcaactcctg ccattgcaga
    22981 gcttgtcgac aatttgtata gttcagttat aacagctggc acatacaaag cagccaatat
    23041 gaaagtggca gaagcggcta aagctattga gaatgcacaa cgagacatga atatatcctt
    23101 tatgaacgaa ttggcattaa tttttgacag agtaggaatt gacaccaatg atgtaattga
    23161 agctgctgga acaaaatgga acttcttgaa ataccatcct ggtttggtgg gtggacattg
    23221 tattagtgtt gacccatatt atttggctca caaagccaaa gctcttggtt atcatcctga
    23281 ggttatactt tctggacgta gggtaaataa tagtatctca gaatttattg cacaaaaaac
    23341 actgaaatta atgattcaga aagggcatac cattaaagat tcaaatgttc taattttagg
    23401 cataactttc aaagaaaatt gtccagatgt acgcaataca aaggtggttg atattcgcaa
    23461 agaactagtt gaatttggtg tcaatgtaga tatgtttgac ccttgggctg aagccgaaga
    23521 agtacacgaa gaatacggaa ttgatttggt gagcaaatta gatgaaacta aaatgtacaa
    23581 tgccattatt gtggctgtag ctcacgatga atttaaagca tttgattttg ctaaatacaa
    23641 gaaacaaggt gcaatcatct atgatgtaaa agcatttgtg gatagggatt tggtagatgc
    23701 gagactttaa taagtggaag tggatagttg aaagttgata gtggaggata tgggaagtct
    23761 tgttggtgaa aaatcgtatg catttgcttt acgcaccatt aaagcgtatc aatatatcca
    23821 aactataaac gaatttgtac tatccaagca actattgaga agtggaacgg caattggagc
    23881 attgataaga gaagctgagc atgcacaaag taaagctgat tttttaaaca aaatgaatgt
    23941 tgcactgaaa gaagcaaatg aaacaatata ttggatttct ttattaaaag atgctaacta
    24001 tatagatgaa aaagtgtata actctattca agcagaagca actgaaattt taaaaatgtt
    24061 aatctcaata gtcaagacaa ccaaacaaaa cttaaacaaa aaatagaatt atcaactatc
    24121 aattatcaac tctcaactat caattaatta aatgaaacaa tatattggat ttctttatta
    24181 aaagatgcta actatataga tgaaaaagtg tataactcta ttcaagcaga agcaactgaa
    24241 attttaaaaa tgttaatctc aatagtcaag acaaccaaac aaaacttaaa caaaaaatag
    24301 aattatcaac tatcaattat caactctcaa ctatcaatta attaaatgaa aatagcaata
    24361 gtaggaacag gatatgtagg tttagtgtct ggtgcatgtt tcgcggaaat tggcacagat
    24421 gttacttgtg tagatgtcga taaaaagaaa atagacgatt tacataatgg aattattcca
    24481 atttatgaac caggcttaga agatattgtt ctaagaaacg taaaagcagg taggttgcat
    24541 tttactactg agttaactga aatattggat gatgttcaaa ttgtgtttag tgccgtggga
    24601 accccaccca atgaagatgg aagtgctgat ttaaagtacg tactacaagt agccaaaacc
    24661 attggtgaaa atatgaataa ctacgttttg ctggttacta aaagtacagt gccggtaggt
    24721 acagcccaac aagtccgaac agttattcaa actgaattag ataaacgtgg ggttgatatt
    24781 gaatttgatg tagcatctaa tcctgagttt ttaaaagaag gagctgctat caaggatttt
    24841 atgagtcctg atagggtggt gataggggta gaaagcgaac gtgctaaaga agtaatgaca
    24901 aaattgtatc gtccattttt gttgaataat tttagggtca tttttatgga tatcccatcg
    24961 gctgaaatga caaaatacgc agcaaatgcc atgctagcaa cacgcatcag ttttatgaac
    25021 gatatagcta atttatgcga attggtagga gctgatgtaa atatggtgcg caaaggaatt
    25081 ggttcggatg cacgtatagg aaataaattt ctgtatcctg gttgtggtta cggtggttct
    25141 tgtttcccaa aagatgtgaa agcactcgta aatacagcca atgagtacgg atatcctatg
    25201 cgtatattac aagccgtaga agatgtaaat ttccaacaaa agcatatatt gttcgagaaa
    25261 ttaaaaaaac actttggcaa taaatttgca gacaagacag tagctatttg ggggttaaca
    25321 tttaaaccca atactgatga tatgagagat gcaccttcgt tggttttaat tgatgagttg
    25381 ttacaagttg gctgcaaagt aaaagcgttc gacccaatta ttactaaatt aacagataag
    25441 gcttataagg atgtgtgcat aggaaaagat atttatgata cagccaacga tgccgatgct
    25501 cttgtattgg ttactgaatg gcaagaattt cgcttgcctg cttggcaagt gatacataaa
    25561 accatgaaaa atcatatagt aattgatggt agaaatatat ttgataaaac tgaaatgagt
    25621 cagtatggtt ttatttacga agggattgga ataaagtagt aattgatgat ttcataaaaa
    25681 atatgcgtta tatatatatt ttcggcaccc ttttttttac agtttatgga caattaatcc
    25741 ttaaatggag attgggtaat ttaagttttt tattgccaca gggaaatata ttagaaaaac
    25801 tagtatcttt gcttaaactt atttttgacc catatatttt ttcaggtttt gcatctgcgt
    25861 ttattgcttc gttattttgg atggcagcga tgactaagtt tcagattaca gaagcctatc
    25921 cctttatgag cttggctcct gctattgttt ttgtattagg aatatggttg ttaggtgaaa
    25981 catttacatg gggaaaagta attggattag tgttaataat tgttggtgtg attataacag
    26041 ttaaattata aaatcattaa aaaaaacaaa caatacaatg caaaataata ttgttttatc
    26101 tgtcgtttct ccagtttatc gtgccgaaaa tatcgtgcag gaattggttc gccagttaca
    26161 tgaacaaata caactgatta cgcagaatta tgagattata ttagttaacg atgcatcgcc
    26221 tgatgcctcg tgggaaaaga ttatcaaagt gtgtgccgaa gataaacgtg taaagggaat
    26281 caacctgagc cgcaatttcg gacagcatta tgcgattaca gcaggactta gttataccaa
    26341 aggcgaatgg gtagtagtaa tggattgcga tctgcaggac aggccggatg aaataccaaa
    26401 tctttacaga aaggctcagg aagggtggga aattgtgttt gcccgaagga cagaaaggaa
    26461 agataagttt tttaaaaggc tttcatcgag gcttttccat caggcttact catatttaag
    26521 cggaattaat actgatagat ctattgcaaa tttcgggata tttcatcaaa aagtaatcgt
    26581 cgaatataac cgaatgaaag aatctgcccg ttctttccca tctttggttc attttctagg
    26641 ctttaagcgc acaacactcg atgttaaaca tgccgaacgt tttgatggta aaaccaccta
    26701 tactttttca aaattaattc gcttgaccac cgatgtaata ctatccaaca gcaataaacc
    26761 gttaaaaatg accgtaaaac ttggtttctc aatctctgtt ctgtcatttt tattagcttt
    26821 gtacaatatt tttgcacact ttgctggaat catcaaagtt gaaggtttta ctacaactgt
    26881 tttttcaatt tggtttgtgg gtggtttaat actgtttgta atgggaatag tgggacttta
    26941 tattggtaaa att
//
LOCUS       DAOSRP010000008        26719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2013_length_26719_cov_5.063456, whole genome shotgun sequence.
ACCESSION   DAOSRP010000008 DAOSRP010000000
VERSION     DAOSRP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26719
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2013_length_26719_cov_5.063456"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PLJ82_01090"
     CDS             <1..1509
                     /locus_tag="PLJ82_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOR40138.1"
                     /translation="SYINKRSWKQIKKTSSFSVTKHAINTRNKLLNIYKKYGITDNEF
                     GIVAALTVGYKEGLSKETKQSFSITGGSHVLAVSGLHVGIIYLALGFMLGFMAKSKQG
                     IVGKQIILIASIWAYAFICGLPPSVVRASIMISLICISEILNRKSATYNAVFVSGFCM
                     LIYNPLYLFDVGFQLSYMAVLSILYFQPRINNIFSPNHIVIKWLWSLTAVSIAAQIGT
                     APISLYYFHNFPTYFLLTNIIVIPAATIIIYLSIVLLLLSSWVKIGLIVAWLLKSCVQ
                     IISIGIHFIEHLPGASIPDIWIPIWFMPIVFLSLVSTILFIRTQRSRYFFLIFYLSIL
                     SISIAIWNRFQTLQNKELIVYANNPSETNFNIHIISGESNTLLADTIYKANLEQYVNT
                     VTHKKHLNPARFEHIDMKNSFGCIDTLTYFVYTNPTKWKPIDISIPVDIVFVYEKRMC
                     HVTEITSHVSPTYIVFGENINGKQLQILKEECEKKSINYHSLYEKGAFILPL"
     gene            1554..2603
                     /locus_tag="PLJ82_01095"
     CDS             1554..2603
                     /locus_tag="PLJ82_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOR40139.1"
                     /translation="MISKIILEDLISQTKKAIDKSTSIVIVSHVNPDGDAIGSSLGLY
                     HFLFSQGKETRIIMPNSFPNFLNFLPDSSDILIYDDYQDEAIDLIESADLVFCLDFNQ
                     LKRVGAMEDVIKNCKATKILVDHHPFPDTFANITISHPEIASTSELIFRMICRMGYFT
                     EMNIHSAECICTGIMTDTGAFSYNSNSPEIYTIISELIKKGVDKDALHRKLYNNYSAQ
                     RMQLMGYLLSQKMKIYAEYGTAIMVLSLAEQETYHHKKGDSEGFVNLPLSIHGITFSI
                     FLKEETDTIKMSFRSQGNFPSNTFANRYFNGGGHLNASGGEGLGTIEETVTYIESILP
                     AFYQEWVDNNNNKEI"
     gene            2616..3824
                     /locus_tag="PLJ82_01100"
     CDS             2616..3824
                     /locus_tag="PLJ82_01100"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOR40140.1"
                     /translation="MKYIIILGDGMADEPIAKLGNKTPLQFANTPYIDKVAELGCSGM
                     LHTIPDGFHPGSEIANLSVLGYKVADVFEGRGSLEAASMGVDIENNEMAMRCNLICIE
                     KGIIKNHSAGHISNEEAAILIADLEKELGNDVVSFYAGVSYRHLLKIKGGNKNITCTP
                     PHDVPGTAFSTVMIEANVPEAQPTADLLNDLILKSQAILDNHPINLKRAAEGKDKANS
                     IWMWSPGYKPKMKTLLELYNLKSGSVISAVDLIRGIGVYAGLKVILVEGATGLYNTNY
                     EGKAQAAIEALRTDDFVYLHIEASDEAGHEGDVDLKVRTIEYLDQRIVKTILEEVSTW
                     SEPVCIAILPDHPTPCALKTHTTDPIPFVIYKPDGKKDDVTVYDEFAARKGGYGEIRE
                     QEFMQLLLQQ"
     gene            3840..5132
                     /gene="thrC"
                     /locus_tag="PLJ82_01105"
                     /pseudo
     CDS             3840..5132
                     /gene="thrC"
                     /locus_tag="PLJ82_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
     gene            5130..5375
                     /locus_tag="PLJ82_01110"
     CDS             5130..5375
                     /locus_tag="PLJ82_01110"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOR40141.1"
                     /translation="MVDGIQNNKNKKTIVLKSYDNPAIANLEHGILQDAGVECFLSDE
                     SVVNLYPMFSSPFGGVKLHVFEEDKEKALEILGTTNS"
     gene            5396..6682
                     /locus_tag="PLJ82_01115"
     CDS             5396..6682
                     /locus_tag="PLJ82_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOR40142.1"
                     /translation="MKQDTKIGTICVQGGWSPKKGESRVLPIIQSTTFKYETSEQMGK
                     LFDLEESGYFYTRLQNPTNDAVAAKIAALEGGVAAMLTSSGQAASFYAISNICSAGDH
                     FVCTSSIYGGTYNLFAVTLKRMGIEVTFVDLDADEQEIAKAFKPNTKALFAETIANPA
                     HAILDIEKFARIAHTHGVPLIIDNTFATPINCRPFEWGADIIIHSTTKYMDGHATAVG
                     GVIVDSGNFDWDAYADKFPGLTQPDDSYHGLTYTKAFGKGAYITKAVVQLMRDLGSCQ
                     SPQNAFLVNIGLETLHLRMARHCENAQKVAEFLENHSKVAWVNYSGLPSNKYYQLAKK
                     YLPKGSCGVLSFGLKAGREASIAFMDNLQLVAIVTHVADARSCVLHPASHTHRQLSDE
                     QLKQAGVAPDLIRLSVGIEDVEDIIADIEQALQKVN"
     gene            6709..7626
                     /gene="metA"
                     /locus_tag="PLJ82_01120"
     CDS             6709..7626
                     /gene="metA"
                     /locus_tag="PLJ82_01120"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOR40143.1"
                     /translation="MPLNIPKKLPAIETLKGENIFVMDDERASTQEIRPLKIVILNLM
                     PIKITTETDLIRLLSNSPLQIEIDFLQIKSHTHKNTPIEHLMAFYKTFDAIKKNKYDG
                     MIITGAPVELMPFEEVNYWNEITEILDWAKTHVTSTLFICWAAQAGLYHYYGIPKYEK
                     PSKVFGVFEHTALDKLNPIFRGFDDSFFVPHSRHTEVKAEDILKHPALTLLSVSKEAG
                     VYMVMARNGREFFVTGHSEYSPNTLDDEYKRDIEKGLPIDLPQNYYIDNNPNNPVNVR
                     WRSHGNLLFTNWLNYFVYQETPYDIDKIK"
     gene            7629..9449
                     /locus_tag="PLJ82_01125"
     CDS             7629..9449
                     /locus_tag="PLJ82_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOR40144.1"
                     /translation="MQTIELLAPAKNKESGVEAILHGADAVYIGAPKFGARAAAGNSL
                     EDIAQLIEFAHIYDAKVYIALNTLIKDDELQEITDLIHSLYAINADALIIQDMGILEL
                     DIPPIALHASTQTDNRTLAKVAFLESVGFDQIVLARELSLQQIKHIAQNSSSKIEVFV
                     HGSLCVSYSGQCYISEALHGRSANRGECAQLCRLPYDLLDANKKIIAKDKHLLSLKDL
                     NLSDHLEELIDAGVYSFKIEGRLKDTAYVKNITAYYRQKIDAIIAKNNNLKRSSTGIT
                     TYTFEPKPEKTFQRSTTSYFFHKPTESVFSVLTPKSTGEYIGTIIDIQKDFFVVDTTI
                     SIANGDGFCYISTKQLLEGFRVNKAIGNRIYPSEMLQMPIGTKIYRNADFAFSKLLHA
                     KSANRRIAISISIQETNVGYLIEFCDENMNKACREIHIDNNKAQKDQQPNIEQQLSKL
                     GNTPFYCKHISIIFTDNKFIPSSVLTQIKQTLSQELLLHRQVRYKPTRAVKTNSASLF
                     PEKELSYTGNVSNNLAHKFYTKHGVIKIDKAFELEKRLPNPVLMFCKACIKDSIGFCP
                     KKNATPFPYKEPLFLKYKEQEFLLSFNCSTCEMTIVQEKI"
     gene            9511..12192
                     /locus_tag="PLJ82_01130"
     CDS             9511..12192
                     /locus_tag="PLJ82_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR40145.1"
                     /translation="MRKLYSKLFALMLVISGFLVPMQLQADVHKRVVLQAFWWNYWNE
                     NYPNSYANYLTELAPRLKEYGINAVWIPPSYKNTGTNSVGYSPFDHYDLGDKYQKGST
                     TTRFGTKDEFLRMVAVMHANGIEVIQDIVLNHVDGAGGTDGAGGEDPSGWDNKWKNFR
                     YVSYATPAKSGNEADYWSRSGRWAKNWKNFHPNNAHNCNSGDICSDWWGPDICYWEGA
                     YGKSSNVVGYNPDQTANYMRNEARNWMMWFKKQTAVDGYRWDAVKHIPDWFQQDMLWN
                     VKYSLPSWSQGGEDMFSVGEYVGSQTEMDNYCSAVQYANGANEFTMGTFDFSLRQGIQ
                     EVIQGGGFGDIGSIPGKQQTKRYANYASQKVHRVVPFVNNHDTFRPILDDDGKYKGWD
                     YSNQIGGNVDPFDKRLGMAYAIIMGVDGNPGIFMEDLFNLSNGKRFSHDPKSETDLPV
                     RSSVRNLIWCHQNLGFKYGDYKIRYQSGDLLVIERAGKAIIAITDNGSTDQEVWIDTD
                     FRNVDMKDYSGSISGTRRAWEDGRFNVKVTNTGTGLGYAVWAPDGITGGYEPYRSKST
                     TQEWEMADDLGDSNCQSLGQGGAIPASTHQRVVGKIYVEENKLVTYNFFPQQDGYDIT
                     LALYDLEGNLLKKQSDNKGFTGEWNATYTGWVTLKVWNNNSENISQKCWVRVKYYAPP
                     TITDVYTDTPDTRVSIWTANGNTSTWNDCNNWEQGKVPANNATVIIPNHTPIVPIMSG
                     NLTLKKLVIEQGKGNIGSPDLIVNGSLTVNELTILSGTAYICGNGTTEVSIQNGSVSN
                     CATRIEEPSFSIEQISVIPNPVESICELRFISNSIGVAYFTLTDVTGKTILTKEIPIN
                     AIGINSTNLYLGGIHTGTYIAKINCNNRIFSVMIVKK"
     gene            12290..12745
                     /locus_tag="PLJ82_01135"
     CDS             12290..12745
                     /locus_tag="PLJ82_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40146.1"
                     /translation="MKKTILIVALLVGFVASGVAQSRSIGLRSFQEVSYQHGFGSSNM
                     LEIDGGFFNTGLQATATYNWLFPIGWGHEGSWNWYVGPGAYAGIRSNSNNNNGLFIGA
                     AGMIGIEYNFWFPLQLSIDYRPTIALFNSNNTPFGFYSNTVGLGVRYIF"
     gene            complement(12830..13261)
                     /locus_tag="PLJ82_01140"
     CDS             complement(12830..13261)
                     /locus_tag="PLJ82_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40147.1"
                     /translation="MYKYFENHPYFKERNVARLNYLDVQQRTLQNEIATLDSLKKLEY
                     FEANSKTKNLKMDNALLIGESNTQLYHTNILEMMEKLQRTKIDIETRNSVVTISSDFQ
                     IKSPQNARVKSLVLFGSAFFVIGFCLALFIRHRNKIKEFLK"
     gene            complement(13306..13767)
                     /locus_tag="PLJ82_01145"
     CDS             complement(13306..13767)
                     /locus_tag="PLJ82_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40148.1"
                     /translation="MEPEKELDLIDLLKLLGNSIGKGISAISRFIMWFIKFCVKNWYI
                     LLVSVAFGIACAFYFSQSKFSKYEGIIVVENNVESTPEFYTEVQSLASYMNNDNTSFS
                     SKFGVSTEIGKKIFRLKPLYIIPAKMKDCIILLTKKRSIQIWSLTNKNLLF"
     gene            complement(13829..14509)
                     /locus_tag="PLJ82_01150"
     CDS             complement(13829..14509)
                     /locus_tag="PLJ82_01150"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR40149.1"
                     /translation="MPIFKENMDIHKRLLYHAFVFPFLFLFIFWLVFLVERVLYTDFT
                     VFGIFPLATKGLLGIITMPFIHADSTHLLANSVTFLVLSVLLFSNYRHIALKVFILIW
                     LFSGVILWVIGRPSWHIGASGVIYGMAFFLFCSGLLRKKIPLVATSLVVVFLYGGMVW
                     YMFPLGIDNHISWEGHLSGALIGMFFSVYYKIKEPSEQKQEDIDENDSVDYDWQENEI
                     ENTTEDTI"
     gene            complement(14510..15463)
                     /locus_tag="PLJ82_01155"
     CDS             complement(14510..15463)
                     /locus_tag="PLJ82_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOR40150.1"
                     /translation="MKLIRFTFLFFVCIVPTIALFSQQYRNPKYQTYIEKFSPIAIEN
                     MQKHGIPASITLAQGILESGIGDSRLAREANNHFGIKCHDWKGEYILHTDDKVNECFR
                     KYKNPEESFEDHSVFLTTRGRYAHLFELKITDYKAWAFGLQRAGYATDPTYATRLINL
                     IEENGLHTFDKQLLQPSKQKSVAKEETKHDTEKPSREHKHDRYPSRPTQRNMYGLRYV
                     IASSNDTYTSIAKEFKVSLRSILKWNDLPYEVSLKEGEIVYLQSKKKRVSSYSTVHYV
                     ASGETMHIISQRYGIRLKSLYDLNNMNYSQVPTIGQKLYLR"
     gene            15699..16184
                     /locus_tag="PLJ82_01160"
     CDS             15699..16184
                     /locus_tag="PLJ82_01160"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOR40151.1"
                     /translation="MKKISLHTAIEVYTYEELTDQQKTLIQKAKETSLSAYSPYSKFS
                     VGAAILLENGEIVCGSNQENAAYPSGICAERTAMFYANSTYPTVAPICIAIVAFTDGK
                     FKQTAISPCGSCRQVLIESEIRFNKPITMLLYGEPEIFFINSITDLLPLNFAPSSLLS
                     S"
     gene            16197..16583
                     /locus_tag="PLJ82_01165"
     CDS             16197..16583
                     /locus_tag="PLJ82_01165"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOR40152.1"
                     /translation="MTKEKISHEYNLRTTISLTHMWQYFHTEDGLGTWFADKVISKNG
                     IYEFFWENTSQKAKILHKTQQFSVRFHWIDDEEETTYFQFKLERDDITNVVTLTITDF
                     SYPDEREDTIQLWNTQLEKLRRTVGL"
     gene            16709..18187
                     /locus_tag="PLJ82_01170"
     CDS             16709..18187
                     /locus_tag="PLJ82_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOR40153.1"
                     /translation="MPIVKKINYYIIKNFLGVFIITFLGSLFILVLQFFLQHINSLVG
                     KGIGLGVFIELFFYAGLTLIPLALPLAILIGSLMTFGNLGERLELLAMKAAGISLWKI
                     MQPLIILVGCISISGFYFSNNILPKTQVKMWTLVFSIREKSPELDIPEGVFYTEIPGR
                     NIFISEKSHKNNTLKDIIIYDFSRGFTNTSIVVADSGKMSFSVDKTNLLISLYKGETF
                     ETLPNNAKTRNAVVPYRRELFEQKEIIIDFDANFNLLGTELLEGQYVSKNASQLQHSI
                     DSISTKIDEEKNKYREDFLRNKYYNSYTGSSSAFAILDSTIDAKGYNFQDILLNTENG
                     LQEQVMLSSINRAYTIKNDLIYNQSIINALIFDARKHSIEWYKKFTLSFACIIFFFIG
                     APLGAIIRKGGMGLPIVISIVLFIVYYIIDNTGYKMAREGIWEVGMGMWFSALVLLPL
                     GMFLTYKAVTDAVILTTEGKLFNPKKWINALSALVKKKKKNI"
     gene            18198..19412
                     /locus_tag="PLJ82_01175"
     CDS             18198..19412
                     /locus_tag="PLJ82_01175"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOR40154.1"
                     /translation="MTTFKLNSIEEAIEDFKEGKFLIVVDDEERENEGDFIIAAEKIT
                     PEAVNFMLINGRGVLCAPITEERCAELELEKQVSNNTSILGTPFTVTIDKIEGCSTGV
                     SAADRAATIKALADPTSTPNTFGKPGHISPLYAKTKGVLRRAGHTEASIDLARLAGLY
                     PAAALIEIMNEDGTMARMPDLQKVAEKFSIKIITIKDLIAYKIKQESIVEKGVTVHMP
                     TQYGMFKLTPFRQKSNGLEHIALTKGEWKDDEPVLVRVHSSCATGDIFGSLRCECGEQ
                     LHHSMKMIEKEGKGAIVYLYQEGRGIGLMNKIKAYALQDKGMDTVDANLHLGFKADER
                     DYGVGASILREIGVRKMKLLTNNPVKRIGLESYGLEIVENIQIVVGINKYNETYMKTK
                     QERMGHNIISSK"
     gene            complement(19464..20846)
                     /locus_tag="PLJ82_01180"
     CDS             complement(19464..20846)
                     /locus_tag="PLJ82_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOR40155.1"
                     /translation="MFVIWLVFVTPSYASSIYPSQSSPLLVSEYESSMQTFDTAIEEM
                     CVAWRMRIKTKETCDSAFRYAPLPYSPDSIRIKRIAMMPTILEMTYNDVVGRYINLYC
                     DRNREISYMLALSQHYFPIFETIFEQYNLPNELKYLSIVESALNPRATSRMGAAGLWQ
                     FMPTTGKLYNLEMNSIYDERRDPIKSTHAAAQHFRDLYEIFQDWNLVIAAYNCGTGNV
                     NKAIRRSGGKKDYWEIYPYLPIETRNYVPAFIAVNYMMYYADSYHICPASIDIPLVTD
                     TLHVTKRIDLLHVSQVLDIPIEQIRLLNPCYKNDIIPDSEKPFILRLPSHLAYLFIEK
                     QDSLYMSQRDVTKQPISLSTSATPHACSSVHIVRSGENLSIIAKRYGITVRQLMNWNN
                     LTSPLVRVGQRLHLNSSKDISVYVVKKGDSLWTIAKNTSVSVEKIKQANRNKNLRILQ
                     PGQQLTIPKN"
     gene            complement(20896..21549)
                     /locus_tag="PLJ82_01185"
     CDS             complement(20896..21549)
                     /locus_tag="PLJ82_01185"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOR40156.1"
                     /translation="MKLKYYIFAIFVFSCLSLWASDDSLVVVSESDSVQEKEVVLYVD
                     STLLHAEFKPQPSKAIWYAAIVPGLGQIYNRKYWKLPILYGGAMGLTYAISWNGGMYR
                     DYQKAYRDIVDNDASTQSYVNILPQGVVIDSDNKAYYTSLLKNKQDNYLRYRDLSIIG
                     TVGLYLLSIVDAYVDAHLYDFDVSPDLSLKIVPKAIPLKHINHFSDVSFGLSCQFSF"
     gene            complement(21562..22437)
                     /locus_tag="PLJ82_01190"
     CDS             complement(21562..22437)
                     /locus_tag="PLJ82_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOR40157.1"
                     /translation="MNKKSGLGRGLGALIEMETVATSGSSSISEVDITKIQANPDQPR
                     TYFDEEALQELATSIKEVGIIQPITLRQVSSDTYQIIAGERRYRASKIAGLTKIPAYV
                     KTVDDEAVMEMALVENIQREDLNAIEIALSYQKLIEISGYTQETMSERVGKKRSTIAN
                     YLRLLKLPAEIQLGITNKKIDMGHARALVTISDASVMLQVYEKIVKEGLSVRKVEELV
                     KNLQSLEVEGEQKVTKSTPRLPAEYTALSKRLSDFLHVSVKIERNESGKGKIILPFRS
                     DEELEGLMDVFDKMM"
     gene            complement(22462..23226)
                     /locus_tag="PLJ82_01195"
     CDS             complement(22462..23226)
                     /locus_tag="PLJ82_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR40158.1"
                     /translation="MGKIIALANQKGGVGKTTTTVNLGASLAVLEKKVLIVDADPQAN
                     ASSGLGIDIEKLEYSIYECLINNVPVSKAIQKTPIEGLHILPSHIDLVGAEIEMLKLD
                     NREKFLRTMLETIKNEYDFILIDCSPSLGLITVNSLTAADSLIIPVQAEYYALEGISK
                     LLNTVKIIKSRLNPSLDIEGFLVTMYDSRLRLANQVYAEVKKHFQDMVFETVIQRNIR
                     LSESPSHGLPVVLYDADSKGAQNHMQLAAEIIKNNS"
     gene            23345..24145
                     /locus_tag="PLJ82_01200"
     CDS             23345..24145
                     /locus_tag="PLJ82_01200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40159.1"
                     /translation="MKISLIISIYKNVPFLKLVLESLQVQSYQDFEIIISEDGEDENV
                     RQFVASYPFKQSYQHLTQKDDGWQKNKALNNAIRKSQGDWLIFIDGDCVLHKRFVEFH
                     AKMADEHLILAGKRVKLSAETSNKLLSNPTLLPTLSRYLYIHFWKSGRYLEEALFIHP
                     QGLLGWITRIRTMKHLKGCNMSFSKKALYAINGFDEDYTLPAIGEDVDILWRLQQAGY
                     QLKSVRNMAVMYHLHHKESWVSQEENKEKMRQKMEQNTYICKNGIQKI"
     gene            24159..25037
                     /locus_tag="PLJ82_01205"
     CDS             24159..25037
                     /locus_tag="PLJ82_01205"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40160.1"
                     /translation="MLSIIICSKFAELSSILTANIHETVGVDYEIIHIDNSQSTHSIF
                     SAYNLGVTKSKFPYLCFVHEDVFFRSSDWGLNIIEHLQTPQVGLIGLAGGCLASRIPA
                     SWSTHGLKMNLIQSGKGKSSYIRTPNQPASTREEVVLLDGVFLATTKENFQFLQFDEK
                     ISGFHGYDYDICMQAKANNLRNYVVYDILLEHFSAGSHNTDYYKNLLLVYKKWINQLP
                     FSCTHLSEAETKLIEKKKLKKLIRKLGIRNFTYSEIKQELDYFATITDNQKVLTWFGF
                     KLFYARLFNNPSRLFK"
     gene            25046..26287
                     /locus_tag="PLJ82_01210"
     CDS             25046..26287
                     /locus_tag="PLJ82_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOR40161.1"
                     /translation="MLEKFRHYVHSINLLCGLFLMASLPFYFPKQQLALTLFFISYIV
                     EFLVDMKWRQIQISATTWYFIGILLFFTMGFLYYPFEDSHTYFQNLTERRLALFGFAI
                     IGIFGYNSLYKLSYFLHTIIISSTLLILYLIIYHIGLPEFLTNENRYDLFTQARIAYV
                     NSHMAFNLYLNIALASVWYLAVTRFKELHWSVKTIYGFSILLIISTLLMSEGRSGFIA
                     AIGLSAIIVIRKIWLTKKIVAIGASVFIVIGMVFAMLQHERMSEKMIEQEPRLFLWTE
                     VALPIIQEKPILGHGLSDGQTQIDSVRQGKQSKILYELWKTNKRVDVHNQFLQTTIEF
                     GLIGLVLLLAIYYYPIRLVNRSRMTLLLLGLFLFSWQSLFDTFITGQFAIIFGFFITL
                     LLTVPKSESLNNNQTALKNLW"
     gene            complement(26250..>26719)
                     /locus_tag="PLJ82_01215"
     CDS             complement(26250..>26719)
                     /locus_tag="PLJ82_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40162.1"
                     /translation="CKASIDYCLTHEDVEEYTFTGDFEEDKHKIRYYQPYTWAELRFL
                     RHSKKLQWETGERRPEPCGLTYPKQIKVLHYQFRSPQQMQQRYAVRQQAKANGCGSFR
                     HEKGNDWKDYLRNRNELVYDKQDGTYQTLGNRNKFNKWHTRITKGFLTLFGYY"
BASE COUNT         9167 a         4585 c         4591 g         8376 t
ORIGIN      
        1 tcatacatca ataaaagaag ttggaaacaa ataaaaaaaa catcctcttt ttcagttacg
       61 aagcatgcta tcaatacccg caataaattg ctcaatatct ataaaaaata tggtataaca
      121 gacaatgaat ttggtattgt tgcagcactg acggtgggat ataaggaagg attatctaaa
      181 gagacaaaac aaagtttttc gattactgga ggctctcatg tattagctgt aagcggctta
      241 catgttggta ttatttatct tgcacttggg tttatgcttg gatttatggc aaaaagcaaa
      301 caagggatag taggcaaaca aattatactc atagcaagta tttgggcgta tgcttttatt
      361 tgcggtcttc ctccatcagt tgtaagagca tcaatcatga tatccttaat ttgtatcagc
      421 gaaatattga acagaaaatc ggctacatac aatgctgtat ttgttagtgg gttttgtatg
      481 ctgatttaca acccgttata cttatttgac gtaggttttc aattaagcta tatggctgta
      541 ttaagcatct tgtattttca gcctcgtatc aataacatat tttcccccaa tcatatagta
      601 atcaaatggt tatggagtct gacggcagtt tctattgcag cacaaattgg aacagctcct
      661 attagtttat attattttca taactttcca acatattttt tacttacaaa cattatagtc
      721 attccagctg ctacaattat tatttatctt tctatagtac ttttattgct ttcttcttgg
      781 gtaaagattg gattaatagt tgcatggtta ctaaaaagtt gtgtgcaaat tatttcaatt
      841 gggatacatt ttattgagca tttaccagga gcaagcatac ctgacatttg gattccgatt
      901 tggtttatgc ctatcgtttt tttatcactt gttagtacca tactttttat tcgcactcag
      961 cgatcaagat atttcttttt aatcttctat ttatctattt tgtccatttc gattgcaatc
     1021 tggaatagat ttcaaacatt gcaaaataaa gaactaattg tctatgcaaa caacccttca
     1081 gaaacgaatt ttaatataca tattatttct ggagaatcaa atacactgtt agcagatact
     1141 atatataagg caaacctcga acagtatgtg aatacagtaa cccataagaa acacttgaat
     1201 ccagcacgtt tcgaacacat cgatatgaag aattcttttg gttgtattga tacactcact
     1261 tactttgtgt ataccaaccc cacaaaatgg aaacccattg acatatctat accagtagat
     1321 attgtttttg tatatgaaaa aagaatgtgt catgtaacag aaattacgtc ccatgtatct
     1381 cccacctaca ttgtatttgg agaaaatata aatggaaaac agctacaaat attgaaagaa
     1441 gaatgtgaaa aaaagagcat taactatcat tcgttgtacg aaaaaggagc atttatactt
     1501 ccactataac aaagtattac tatttttgca gtagatttac taatagtgat aatatgatat
     1561 caaaaattat tctcgaagat ttaatttcac aaacaaaaaa agcaattgat aaatccacta
     1621 gtattgtgat tgtatctcat gtcaaccctg atggagatgc cattggtagt tctttagggc
     1681 tgtatcattt tttgttttct cagggaaaag aaactcgtat tattatgcca aattctttcc
     1741 ctaatttcct taacttttta cccgacagta gtgatatctt gatatatgac gattatcaag
     1801 atgaagctat cgacttaatt gaatctgccg atttagtttt ttgtctggat tttaatcaac
     1861 taaaacgtgt aggtgcaatg gaagatgtaa taaaaaattg caaagcaaca aaaatattag
     1921 tagatcatca cccatttcca gatacctttg ctaacattac tatatcacat cccgaaattg
     1981 cctccacaag tgagttaatt tttcgtatga tttgtaggat gggatacttc actgaaatga
     2041 acattcattc agcagaatgc atttgtacgg gtattatgac cgatacagga gctttttcgt
     2101 acaattccaa tagtcccgaa atctatacca ttatttcaga attgataaaa aagggtgttg
     2161 ataaagatgc attacacaga aaattatata ataattattc agcacaacgg atgcaattaa
     2221 tgggatattt attgtcgcaa aaaatgaaaa tctatgctga atacggaact gcaatcatgg
     2281 ttttgtcttt ggcagaacaa gaaacctatc accacaaaaa aggagatagc gagggttttg
     2341 ttaatttgcc attatccata catggcatca cattttctat ttttctgaaa gaagaaacag
     2401 acactataaa aatgtcgttc cgctcgcaag gtaattttcc aagcaatacg tttgccaacc
     2461 gttattttaa tggtggtggt catttaaatg cctctggggg tgaaggttta ggtacaatcg
     2521 aagaaacagt tacgtatatt gaatccatat tacctgcatt ttatcaggaa tgggttgata
     2581 ataacaataa caaagaaata taaacttata cttacatgaa atatattatc atacttggag
     2641 acggaatggc tgatgaacca atagctaaat taggaaacaa aacacccttg caatttgcaa
     2701 atactccgta tatcgacaaa gtagctgaac ttggttgtag cggcatgcta cataccatac
     2761 ctgatggctt tcatccaggt agcgaaattg ccaatttaag cgtactcgga tacaaggtgg
     2821 ctgatgtgtt tgaaggaaga ggttcgttag aagcggctag tatgggcgtt gacattgaaa
     2881 ataatgaaat ggcaatgcgt tgcaacctta tttgtataga aaaaggcatt atcaagaacc
     2941 actctgctgg acatataagc aacgaagaag cggcgatttt gattgctgat ttagaaaaag
     3001 aattgggcaa tgatgtggtg agtttttatg caggtgtatc gtatcgtcat ttactaaaaa
     3061 ttaaaggagg aaataaaaat atcacttgta ctcctccgca tgatgtaccg ggcacagctt
     3121 tttctactgt tatgattgag gcaaatgtgc ccgaggcaca accaacagcc gacttattaa
     3181 acgatctaat tttaaaatcg caagcaattt tggataacca cccaattaac ctaaaacgag
     3241 cagctgaagg gaaagacaaa gcaaatagta tttggatgtg gtcgccagga tacaaaccaa
     3301 aaatgaaaac cctgcttgaa ctgtataatt taaaaagtgg ttcagtaata tcagcggtag
     3361 atttaatccg tggaattggc gtgtacgctg gcttaaaagt tattttagta gaaggggcta
     3421 ctggcttata caacacaaat tacgaaggaa aagcacaagc agcaattgaa gcattacgca
     3481 ctgatgattt tgtgtaccta cacatcgaag caagcgatga agctggtcac gaaggggatg
     3541 ttgacttaaa agtaagaacc attgaatacc tcgaccaacg aattgtaaaa acaatattag
     3601 aagaagtaag cacttggagc gaacctgtat gcattgctat tttacccgat caccctaccc
     3661 catgtgcttt aaaaacacat acaacagacc ccatcccttt tgttatttat aaacctgatg
     3721 gaaaaaaaga tgatgtaaca gtgtacgatg aatttgctgc acgcaaaggg ggctacggag
     3781 aaattcgaga acaagaattt atgcaattat tattacaaca ataaacctat atagtcaata
     3841 tgaattatta cagcaccaat aaaaatgcat ctatagctac cttacaaaaa gcagttgtaa
     3901 aaggcttagc tgaagacaaa ggcttgtata tgccagaaac tattaagtta ttacctccgc
     3961 aattttatga caacatagat agtatgtctt ttcaagaaat tgcttacgaa gtggctcatg
     4021 ctttttttgg agaagacatt gaggagggag ctttaaagca aattgtgtac gacacattaa
     4081 actttgacgt acctctggtt cctgttaccg aaactattta cagtttagaa ttataccacg
     4141 gacccacctt ggcttttaaa gatgtaggtg cacgttttat gtcgcgtttg cttggctatt
     4201 ttattgaaaa agaaggagaa aaaaatgtaa aagtattggt tgccacttct ggcgacacag
     4261 gcagtgctgt agctaatgga tttctcggtg ttaaaggtat tcatgtatat gtactgtatc
     4321 ctaaaggatt ggttagccct attcaagaat gtcagtttac tacattagga caaaatatta
     4381 cagccataga agtagacgga acatttgacg actgccaagc attggttaag aatgctttta
     4441 tggacaaaga actcaatgaa gcattaaaac ttacatcagc aaactcgata aacgtagcac
     4501 gctttttgcc acaggcattt tattactttt atgcatatgc tcagttgaaa aagattggga
     4561 aagaacaaaa catggttgta tgtgtaccaa gcggtaattt tggtaatatt acagcaggtt
     4621 tgtttgcaaa aaaaatggga ttgcctatca agcgttttat tgcagcgaac aatcgtaacg
     4681 atatattctt acagtattta caaacgggtg tgtacacccc aaaaccatcg gtagccacca
     4741 tagccaatgc aatggatgta ggcgacccaa gtaactttgc acgtatcctt gacttgtata
     4801 agaactcaca cgaagctatt tgtgctgaca ttagtggatc aagttataca gatgaagaaa
     4861 tagccgatac gttaaaaaaa tgctatcagc aaaacgatta tttgcttgat cctcatggag
     4921 cttgtgcata ccgtgctctt acagaacaga taaaagaaaa tgaaattgga gttttcttgg
     4981 aaacagctca tccagctaaa tttcttgaaa ccgttcagaa tataattaaa caagaagttg
     5041 taattcctga aaaactccaa gagtttatga aaggaaaaaa agaggttgaa ccaatgagca
     5101 aagaattttc tgctttcaaa gcttttttga tggtcgatgg aatacaaaac aataaaaata
     5161 aaaaaacaat cgttcttaaa tcgtacgata acccagctat tgcaaattta gaacatggca
     5221 tactgcaaga tgcaggagtt gagtgtttct tgagcgacga aagtgtggta aatctgtatc
     5281 caatgttttc ttctcctttt ggtggagtta aactgcatgt ttttgaagaa gataaagaaa
     5341 aagcattgga aatactagga acaactaatt cgtaattatt aacaaaagaa taacaatgaa
     5401 acaagataca aaaattggga ctatatgtgt acagggcgga tggagtccta aaaaaggaga
     5461 atcgcgtgta ctacctatca tacaaagtac tacctttaaa tacgaaacga gcgaacaaat
     5521 gggcaagctc tttgatttgg aagaaagtgg ctatttttat actcgtttac aaaacccaac
     5581 caacgatgca gtagctgcaa aaatagctgc cttagaaggt ggcgtagctg ctatgctaac
     5641 ctcttcgggt caagctgcat cattttacgc aatatcaaat atatgttcgg ctggagatca
     5701 ttttgtatgt acttctagca tttatggagg cacatacaat ttgtttgctg ttacactcaa
     5761 gcgtatgggt attgaagtaa catttgttga tttagatgca gatgaacaag aaatagcaaa
     5821 agcatttaaa ccaaatacaa aagctttatt tgctgaaaca atcgctaatc cagctcatgc
     5881 aattttagat atagaaaagt ttgctagaat tgcacataca catggtgtgc cattaattat
     5941 tgacaataca tttgcaacac ctatcaattg tcgcccattc gaatggggag cagatattat
     6001 tatccattcc actaccaaat atatggatgg acacgccacc gcagtaggtg gtgtcatcgt
     6061 agatagtggc aattttgatt gggatgcata tgctgataaa tttccgggat taacacaacc
     6121 tgatgattca tatcatggat taacatatac caaagctttt ggcaaaggag cttatattac
     6181 aaaagctgtg gtacaattaa tgcgtgattt aggttcatgt caatcgcccc aaaatgcttt
     6241 tttagtgaac attggattag aaaccttgca tctacgcatg gctcgacatt gcgaaaatgc
     6301 acaaaaagta gcagaatttt tagaaaatca tagcaaagtc gcttgggtaa attatagtgg
     6361 attaccctcg aataaatact accagttagc taaaaaatat ttaccaaaag gttcgtgtgg
     6421 tgtattatct tttggattaa aggctggaag agaggcatct atcgcattta tggataactt
     6481 gcaattagta gctattgtta cacacgtggc agatgctcgt tcgtgcgtgc tacatccagc
     6541 aagccataca caccgtcaat tgtctgatga acaacttaaa caagcaggtg tagcacctga
     6601 cttaattcgt ctttcagtag ggatagaaga tgtagaagat atcattgctg atattgaaca
     6661 agcactgcaa aaagtaaatt aatgtatcac taaaaaaata aacacatcat gccattaaat
     6721 attcctaaaa aactccccgc aatagaaaca ttaaaaggag aaaacatttt tgtaatggac
     6781 gatgaacgtg catcaaccca agaaattaga ccacttaaaa tagtgattct taacttaatg
     6841 cctattaaga taacaaccga aacggattta attcggttat tgtcaaactc gccattgcaa
     6901 atagaaattg attttttgca aataaaaagt catacacaca aaaatacgcc tatagaacat
     6961 ttaatggctt tttataagac gtttgatgca ataaaaaaga acaaatacga tggaatgatt
     7021 atcactgggg ctccggtaga attaatgcct tttgaagaag taaattattg gaatgaaata
     7081 actgaaatac tcgattgggc taaaacgcac gttacttcta ccctctttat ttgttgggca
     7141 gcacaagctg gtttgtatca ttattatggc atacctaaat atgaaaaacc aagcaaagta
     7201 tttggtgtgt ttgaacatac agcattagac aaacttaatc ctatatttcg cggatttgac
     7261 gacagcttct ttgtgcctca tagccgacac accgaagtaa aagcagaaga tattctcaaa
     7321 catcccgcct taaccttatt atccgtgtcg aaagaagccg gagtatatat ggtaatggct
     7381 cgtaacggca gagaattttt tgtaacagga cattctgagt attctcctaa tacattagat
     7441 gatgaatata aacgagatat agagaaagga ttacctattg atttaccgca aaattattat
     7501 attgacaata atccaaacaa tcctgttaat gttcgttgga gaagccatgg taatttactg
     7561 tttactaatt ggttaaatta ttttgtgtac caagaaacac cttacgatat agacaaaatt
     7621 aagtaatcat gcaaaccatc gaacttcttg cccctgctaa gaacaaggaa agtggggttg
     7681 aagccatatt acatggtgca gatgcggttt atattggagc tccaaaattt ggagcaaggg
     7741 ctgctgctgg aaattcgcta gaagatattg ctcaacttat cgaatttgct catatatatg
     7801 atgcaaaagt atatattgca ctaaatacat taattaagga tgatgagctg caagaaatta
     7861 cagatctaat tcactcttta tatgcaatta atgcagatgc acttattata caagatatgg
     7921 gcattttgga acttgacata ccgcctattg cgttgcatgc tagtacacaa acagataata
     7981 ggacattagc taaagttgca tttttagagt ctgtaggatt cgaccaaata gtattggcac
     8041 gagaattatc gttacaacaa atcaaacata ttgctcaaaa tagctctagt aaaatagaag
     8101 tatttgtgca tggctcatta tgtgtaagct acagcggaca atgctatata agcgaagctt
     8161 tgcatgggcg aagtgccaat cgaggtgagt gtgctcaatt atgtaggctt ccatacgact
     8221 tattagatgc taataaaaaa ataattgcaa aagataagca tcttttatcg cttaaagatt
     8281 taaatttatc tgatcacctt gaagaactaa ttgacgctgg agtttattcg tttaaaatag
     8341 agggacgatt aaaagatact gcatatgtta aaaatattac ggcttactac agacaaaaaa
     8401 tagatgcaat tattgcaaaa aataataatc taaaacgctc atcaactggc ataacaacct
     8461 acacatttga acctaaacca gagaaaacct tccaacgaag tactacatcc tatttttttc
     8521 acaaacctac tgaatcagta ttttcagttt taactccaaa atcaactggg gaatatattg
     8581 gtaccataat tgatatacag aaagattttt ttgtagttga taccactata tccatagcaa
     8641 atggtgatgg attttgctat atatctacaa aacaactact ggaaggattc agagtaaaca
     8701 aagctattgg aaaccgcatt tatccatccg aaatgctaca gatgcctatt ggaaccaaaa
     8761 tatatagaaa tgctgatttt gctttttcaa aattactcca tgcaaaatca gcaaatagaa
     8821 gaatagctat ttctatctca atacaagaaa caaatgtggg gtatttaatt gagttttgtg
     8881 atgaaaatat gaataaagca tgtagggaaa tacatattga taacaacaaa gcacaaaaag
     8941 atcagcaacc aaatattgaa caacaacttt caaaattagg aaatacacca ttttattgca
     9001 aacatatctc cattatattt acggataata aatttattcc ttcttctgta ttaacacaga
     9061 taaaacaaac tctttcacaa gaattacttt tacatagaca agtaagatat aagccaacaa
     9121 gggcagtaaa aacaaactcg gcatccttat tccctgaaaa agaattaagt tatacaggca
     9181 acgtaagcaa taacctagca cataaatttt ataccaaaca tggggttata aaaatagaca
     9241 aagcctttga acttgaaaaa aggctaccca accccgtact tatgttttgt aaagcatgta
     9301 tcaaagactc aataggattc tgtcccaaaa aaaacgcaac tcccttccct tataaagaac
     9361 ctttattttt aaaatataaa gagcaagagt ttctgttatc atttaactgc tcaacatgtg
     9421 aaatgactat tgttcaagaa aaaatataat tttttttgga ataatacaat tttcattcta
     9481 tatttgtaag ttacttaata ccttctactt atgagaaaat tgtacagtaa attatttgcc
     9541 ttgatgctag taattagtgg atttctcgtt ccaatgcaac tacaagctga tgttcataaa
     9601 agagtcgtat tgcaagcatt ttggtggaac tattggaatg aaaactaccc aaatagttat
     9661 gcaaactacc ttactgagtt agctcctagg ctaaaagaat atggaattaa tgctgtatgg
     9721 atacctcctt cttataaaaa tactggaaca aattctgttg gatattcccc ttttgatcat
     9781 tatgatttgg gagataagta tcaaaaaggt tctactacca cacgttttgg aactaaagac
     9841 gagtttttac gtatggttgc ggtaatgcat gccaatggaa ttgaagtaat ccaagacatt
     9901 gttttaaacc acgttgatgg tgcaggaggt actgacggtg caggaggaga agacccctct
     9961 ggctgggata ataaatggaa aaattttagg tatgtatcgt atgctactcc agccaaaagt
    10021 ggcaatgagg cagattattg gagtagaagt ggaagatggg caaaaaattg gaaaaacttc
    10081 catccaaaca atgcccacaa ttgtaatagt ggagatatct gttctgattg gtggggacca
    10141 gatatttgtt actgggaagg agcatacgga aaaagttcta acgtagtggg ttacaatcca
    10201 gaccaaacag caaactacat gcgaaacgaa gcacgtaatt ggatgatgtg gtttaaaaaa
    10261 caaacagcag ttgatggtta tcggtgggat gcagtaaaac acatacctga ttggtttcaa
    10321 caagacatgc tttggaatgt gaaatactcc ttgcctagtt ggagtcaagg aggcgaagat
    10381 atgtttagtg taggcgaata tgttggtagt caaaccgaaa tggataatta ttgtagtgcc
    10441 gttcaatacg caaatggagc taatgaattt actatgggaa catttgattt ttctctcaga
    10501 caaggtattc aagaagttat tcaaggagga ggttttggtg atattggatc aattcccgga
    10561 aaacaacaaa caaaacgcta tgccaactac gcttctcaaa aagtacatag agtagtacct
    10621 ttcgttaata accatgacac cttcagacca atattagatg atgatgggaa atataaaggc
    10681 tgggattaca gcaatcaaat aggaggaaat gtagatcctt ttgacaaacg tttgggaatg
    10741 gcatacgcca taataatggg agttgatggc aacccaggca tatttatgga agatcttttt
    10801 aatctaagca acgggaagag atttagtcat gacccaaaaa gtgaaacaga cctgcctgtg
    10861 cgaagttctg ttaggaacct tatttggtgt catcaaaatc ttggatttaa atatggcgac
    10921 tacaaaatac gttatcaatc aggcgattta ctagtaattg aacgtgcagg aaaagcaata
    10981 attgctatta ctgataatgg ctctaccgac caagaagtat ggatagatac agactttcgt
    11041 aatgtagata tgaaagatta ctctggtagc attagcggta cacgaagagc atgggaagac
    11101 ggacgtttta atgtaaaagt aacgaataca ggaacaggat taggttatgc cgtatgggct
    11161 ccagatggga taactggcgg atacgaacca taccgatcca aatcaacaac acaagaatgg
    11221 gaaatggctg atgacttagg cgatagtaat tgccaatcgt taggacaagg aggagccatt
    11281 cctgcatcaa cgcatcaacg tgtagttggc aaaatatatg tagaagaaaa caagcttgta
    11341 acctacaact ttttcccaca acaagatgga tatgatataa cattggcatt gtatgactta
    11401 gaaggaaatc tcttaaagaa acaaagtgat aacaaaggat ttactggaga atggaatgca
    11461 acttatacag ggtgggtaac actgaaagtg tggaataata attctgaaaa tatctcacaa
    11521 aaatgttggg taagagttaa atactatgct ccgcccacta ttacggatgt gtatacagat
    11581 acccctgata cacgagtaag tatttggaca gcaaacggta atactagcac atggaacgat
    11641 tgtaataatt gggaacaagg caaagttcca gctaataatg ctacggttat cattcctaat
    11701 cacaccccaa ttgtgcccat tatgtctgga aatcttacat taaaaaaatt ggttattgag
    11761 caaggtaaag gcaatattgg aagtcctgat ttgatagtta atggttcttt aacagtaaac
    11821 gaattgacta ttttatctgg tacagcatat atttgtggca atggtacaac agaggtatct
    11881 attcaaaatg gttctgtatc aaattgtgca acacgtattg aagaaccttc ttttagtatt
    11941 gaacagatat cagtcatacc caatccagta gaatctatct gcgaattgcg ttttatttcc
    12001 aatagtatcg gtgtagctta tttcacatta actgatgtaa ctggaaaaac aattctgaca
    12061 aaagaaatcc ctattaatgc tattgggatc aattctacca acttatattt aggaggtata
    12121 catactggaa catatattgc caaaataaac tgtaacaacc gaattttttc tgtaatgatt
    12181 gttaaaaaat aaggaataac aactaatttt aattgctttt tcagaaaata aacctttact
    12241 ttgcacgcga aaaagtaaag gtttattttt taactaaaac gaaaaaatta tgaaaaaaac
    12301 gattttaatt gtagccttat tagtaggctt tgtagcaagt ggtgtagcac aatcacgttc
    12361 aataggatta cgatctttcc aagaagtaag ttatcaacat ggatttggta gtagcaatat
    12421 gctagaaatt gacggagggt tctttaatac aggtttacaa gcaaccgcta catacaattg
    12481 gttatttcca attggttggg gtcatgaagg cagttggaat tggtatgtag gtccaggtgc
    12541 atatgcaggt attcgctcga acagtaataa caataatgga ttatttattg gagcagcagg
    12601 tatgattggt atcgaatata acttttggtt tcctcttcaa ttatcaatag attatcgccc
    12661 aacaattgct ttatttaata gtaataatac gccttttgga ttctactcaa atactgttgg
    12721 attaggagtt aggtatattt tctaacaaaa ttacaagtgt atagtcttta taaaacaata
    12781 gcgagttcaa aaagttgaac tcgctattgt tttataacta atgaacgtgt tattttaaaa
    12841 actcctttat tttgttccta tgacgaataa atagagcgag acaaaaacca ataacaaaga
    12901 aagcagaacc aaataaaact aaagacttta ctctagcatt ttggggagat tttatctgaa
    12961 aatcagaact aatcgttact acgctatttc ttgtttcaat atcaattttc gtacgctgca
    13021 atttttccat catttctaaa atattagtat gatacaactg cgtattggac tctcctatta
    13081 ataaagcatt atccattttt aaattttttg ttttagaatt agcttcgaaa tattccaatt
    13141 ttttcaaact atccaacgtc gctatttcat tttgcaatgt gcgttgttgc acatctaaat
    13201 aatttaaccg agccacattc cgctctttaa aatagggatg attctcaaaa tatttgtaca
    13261 atgcggtctt taagaacggg aataacgaac gatcatttgt ctttattaaa atagcaaatt
    13321 tttgttggtg aggctccaaa tctgaatact tctttttttc gtcaataaaa ttatacaatc
    13381 cttcatcttc gctggtataa tataaagagg tttcaaccta aatatctttt tcccaatttc
    13441 agttgaaaca ccaaatttag acgagaaaga tgtattatca ttgttcatat aagatgccaa
    13501 agactgtact tctgtataaa actcaggggt actttctaca ttattctcta ctacaattat
    13561 tccttcatat tttgaaaatt tagattgtga aaaataaaaa gcacaagcta ttccaaaagc
    13621 tacggaaaca agtagtatat accaattttt tacacaaaac ttgataaacc acataataaa
    13681 tctactaata gcagatattc ctttacctat cgaattgcct aacaatttta ataaatcaat
    13741 taaatctaat tccttttctg gttccatact gtttctgttg tttatatttg taactctttt
    13801 gatgcacaaa aatacataaa atattgattt aaatagtatc ttctgttgta ttttctatct
    13861 cgttctcttg ccaatcataa tcaacactat cattttcatc tatatcctct tgtttttgtt
    13921 cagatggctc tttgatttta taataaacag aaaaaaacat acctatcaac gctcctgaca
    13981 aatgcccttc ccacgaaata tgattatcaa tacctaaagg aaacatatac cacaccatac
    14041 ctccatatag aaaaactaca accaaagaag tagcaactaa gggtattttt tttcgaagca
    14101 atccactaca aaaaagaaaa aaagccattc cataaattac tccacttgcc cctatatgcc
    14161 aacttggcct accaataacc cataatatta cgcctgaaaa aagccagata agaataaata
    14221 cttttagtgc aatatgccta taattagaaa acaacaaaac agataaaacc aaaaaagtga
    14281 cggaattagc taggagatgt gtgctatctg catgaataaa tggcattgtt ataattccca
    14341 ataacccttt agttgctaga ggaaatatgc caaatacagt aaaatcagta tagagtaccc
    14401 tctctaccaa aaatacaagc caaaaaataa aaagaaataa aaaaggaaaa acaaacgcat
    14461 gataaagtaa acgtttatga atatccatat tttctttaaa aataggcatc tatcttaagt
    14521 ataatttttg accaatagtt ggtacctgag aataattcat attattcaaa tcatataacg
    14581 attttaatcg tattccataa cgttgagaaa ttatatgcat cgtttctcct gaggcaacat
    14641 aatgtactgt gctataactc gacacgcgtt ttttcttgct ttgcaaataa acaatttctc
    14701 cttctttcaa acttacctca tagggcaaat cattccattt gagtatagaa cgtaaagata
    14761 ccttaaattc cttagctata gatgtatacg tatcattaga agaggcaatc acataacgta
    14821 atccgtacat attccgttgt gttggtcgtg atggatatct atcatgcttg tgttctcgtg
    14881 atggcttttc agtgtcgtgt ttcgtttctt ccttagcaac tgatttttgc ttagaaggct
    14941 gtagaagctg tttatcaaat gtatgcaatc cattttcttc aattaagttg attaatctgg
    15001 tggcataagt tggatctgtt gcataacctg cacgttgtaa accaaaggcc caagccttat
    15061 aatcagtaat tttcaactca aataaatgag catagcgacc tcttgtggtt aaaaataccg
    15121 aatgatcttc gaacgattct tcaggatttt tatattttct aaaacattca tttactttat
    15181 catcagtatg taaaatatat tcccctttcc aatcatgaca tttgatacca aaatgattgt
    15241 tggcttcacg agccaaacga ctatccccga tacctgactc caaaatccct tgagccaatg
    15301 taatacttgc cggaattccg tgtttctgca tattttctat agcaataggg ctaaattttt
    15361 cgatataagt ttggtatttg ggatttctat attgctgaga aaaaagagct atcgttggaa
    15421 cgatacatac aaaaaaaaga aaggtaaatc gtattaattt catgcttagg aactctcact
    15481 atttaaaatt ataaacccat taataatcgt tgataaaata atagatacaa aaatagtatt
    15541 ttagttacga aaaatgctaa aaaaattgtt tttttagtga attttagtta tgtttgtaaa
    15601 atatggatag tatgatataa aagtttctaa ttgatagaaa atttatctaa cgaagcaaat
    15661 actaaataaa tctgtactaa aacattaatt gtaacataat gaaaaaaata agtttacata
    15721 cggcaattga ggtatataca tacgaagagc ttaccgacca acaaaaaaca ctcatacaaa
    15781 aagccaaaga aacctcttta tctgcctata gcccatattc aaaatttagt gttggtgctg
    15841 ctattttact agaaaatggg gaaatagttt gtggctctaa tcaagaaaat gcagcgtatc
    15901 cctcaggcat atgtgctgaa cgaacagcta tgttttatgc aaattctact tatccaacgg
    15961 ttgctcccat ttgtattgct attgtagctt ttactgacgg aaaatttaaa caaacagcta
    16021 tatctccatg tggatcatgc agacaagtac taatagaatc tgaaattcgt tttaataaac
    16081 ctataactat gctgctatat ggagaacctg aaattttttt cattaacagc ataacagact
    16141 tattgcctct caattttgct ccttctagtt tgttatcttc ataataccaa aagcttatga
    16201 caaaagaaaa aatctctcac gaatataacc ttcgtacaac catttcgcta acacatatgt
    16261 ggcaatattt tcatactgaa gatggtttgg gcacttggtt tgcagacaaa gtaattagta
    16321 aaaatggcat atacgaattt ttttgggaaa acacttccca aaaagcaaaa atactgcata
    16381 aaacacaaca attttctgtt cgattccatt ggatagatga tgaagaggaa actacttatt
    16441 ttcaatttaa attagaacgt gatgatataa caaatgttgt aacacttact atcactgatt
    16501 tttcatatcc agatgaacga gaagatacaa tacaactatg gaatacccaa ctggaaaaac
    16561 ttcgtagaac ggttggttta taaaaacatt tgattcaaaa ataacaacag aatacgtttt
    16621 gttaacactt taaatactaa aattgcatat ttttctgtaa ttttgtgctc tatttcgtga
    16681 aatgggtaaa aacatacagc aattaagaat gccaattgta aaaaaaataa attattatat
    16741 tataaagaat tttctaggag tatttattat tactttttta gggagtttat ttatccttgt
    16801 actccagttc tttttacaac acattaacag tttggttggg aaaggcattg gactaggagt
    16861 gtttattgaa ttattttttt acgcagggct aacactcatc cctcttgctt tacctttagc
    16921 aattttaatt ggctcattga tgacctttgg gaatttagga gaacgattag agctactggc
    16981 aatgaaagct gcaggaattt ctctttggaa aattatgcaa cctcttatta tactggttgg
    17041 ttgcatatca atctctggat tttatttttc taataatatt ctacccaaga cccaagtaaa
    17101 aatgtggacc ttagtattct ccatacgaga gaaatctcct gaattagata tacctgaggg
    17161 agttttttat acagagatac ctggtagaaa tattttcata agtgaaaaga gccataaaaa
    17221 caatacactt aaagacatca ttatatacga tttctctaga ggatttacca atacaagtat
    17281 tgtggttgct gactctggaa aaatgagttt tagtgttgac aaaacaaatc ttcttatttc
    17341 tttgtacaaa ggggaaacct ttgaaacatt accaaataac gcaaaaacta gaaatgctgt
    17401 tgtcccatat cgcagagaat tatttgaaca aaaagaaatt atcattgatt ttgatgcaaa
    17461 cttcaatttg cttggaacag aattacttga aggtcaatat gtaagcaaaa atgcatcgca
    17521 actacaacac tctatcgact ccatttccac aaaaatagac gaagaaaaaa acaaatatcg
    17581 ggaagatttt ttgcgaaata agtactataa ctcatacaca ggatcttcct cggcttttgc
    17641 aattttagac tcaaccatag atgctaaagg atataatttt caagacattc tccttaatac
    17701 agaaaatggt ttgcaagaac aagttatgct atcttctatt aatcgagcat acactataaa
    17761 aaatgattta atatacaatc aatccattat aaatgctctt atatttgacg ctcgtaaaca
    17821 ctccattgaa tggtataaaa agtttacact ttcttttgca tgtatcatat tcttttttat
    17881 aggagcacca ttaggagcca tcatccgcaa agggggaatg ggtttgccta tcgttatttc
    17941 aattgtgcta ttcattgtat attacataat tgataatact ggatacaaaa tggctcgtga
    18001 aggtatatgg gaagtaggta tgggaatgtg gtttagtgca ttggtattgc tccccttggg
    18061 tatgttctta acctataaag cagttactga cgctgttatt cttaccaccg aaggcaaatt
    18121 atttaatcca aaaaaatgga taaacgctct aagtgcatta gtcaagaaaa aaaagaaaaa
    18181 tatttaacta aaaaaatatg actacgttta aattaaattc tattgaagaa gcaatagaag
    18241 atttcaaaga aggtaaattc ctaattgtag tagacgatga agaaagagaa aatgaaggag
    18301 acttcattat agctgccgaa aaaattacac cagaagctgt aaattttatg ttaattaatg
    18361 gtcgtggtgt gttgtgtgct cctatcacag aagaacgttg tgccgagctt gaattagaaa
    18421 agcaagtttc aaacaatacc tcaatacttg gaactccttt taccgttacc atagataaaa
    18481 ttgaaggttg ttcgacggga gtgtctgctg ctgacagagc cgctacaatc aaggctcttg
    18541 ccgatcctac ttctactcca aatacatttg gcaaacctgg acatattagt ccgctatacg
    18601 caaaaaccaa aggcgtactc agacgtgctg gacacaccga agcatctatt gatttagctc
    18661 gtttggcagg cttgtatcct gctgcagctc taatcgaaat aatgaacgaa gatggcacaa
    18721 tggcacgtat gccagatttg caaaaagtag cagagaaatt tagtataaaa ataattacaa
    18781 ttaaagattt aatcgcctat aaaattaaac aagaatcaat tgttgaaaag ggtgtaactg
    18841 tacatatgcc tactcaatat ggaatgttta aactaactcc gtttcgccaa aaatcaaatg
    18901 gattagagca cattgcatta accaaaggag aatggaaaga cgatgagcct gttttagtac
    18961 gtgttcactc atcgtgtgct acgggagata tttttggctc attgcgttgc gaatgtggtg
    19021 aacagctaca tcatagtatg aaaatgattg aaaaagaagg aaaaggtgca atcgtatatc
    19081 tttatcaaga aggaagaggt attggtttaa tgaacaagat taaagcgtat gccctacaag
    19141 acaaggggat ggatactgta gatgctaatt tacatcttgg ctttaaagct gacgaaagag
    19201 attatggtgt aggtgctagt atcctaagag aaataggtgt gagaaaaatg aagcttctta
    19261 cgaataatcc tgtaaaaaga attgggttag aatcttacgg attagagatt gtggaaaata
    19321 ttcaaattgt ggtgggtatt aataaataca atgaaaccta tatgaaaacg aaacaagaaa
    19381 gaatgggaca taatatcata tcgtcaaaat agccctccat aactaaaaca atacaaagga
    19441 atcaaactat acaattggtt cctttaattt tttggaatag tcagttgctg acctggttgc
    19501 aaaatacgca aatttttatt tctattagct tgttttatct tctctaccga gacacttgta
    19561 tttttggcaa tagtccatag gctatctcct ttttttacca catagacaga tatatcttta
    19621 gacgaattga gatgtaaacg ttgccctaca cgaactaatg gtgaagttaa attattccag
    19681 ttcattaatt gacggacggt tataccataa cgtttggcaa taattgacaa attttctcca
    19741 gaacgtacaa tgtgtacaga tgaacatgcg tgaggagtag ctgatgttga tagagaaatt
    19801 ggttgtttag tcacatcacg ttgcgacata tatagagagt cttgcttttc tataaataaa
    19861 tacgctaaat gagaaggaag tcgtaaaatg aaaggttttt ctgaatcagg aattatatca
    19921 tttttataac agggattaag taaccgtatt tgctcaatcg gtatatccaa cacctgagaa
    19981 acatgtaaca aatctatcct ttttgtaaca tgaagagtat ctgttaccaa tggaatatca
    20041 atgctagctg gacaaatatg atagctatct gcataataca tcatataatt cactgcaata
    20101 aatgcaggca cataattacg tgtttcaata ggtaaatatg gataaatctc ccaataatct
    20161 ttttttccac ccgatcttct gatagcttta ttaacatttc cagttccaca attataagcg
    20221 gctatcacta aattccaatc ctgaaatatt tcatataaat ctctaaaatg ctgggcagct
    20281 gcatgagttg atttgatggg gtctcttcgc tcatcatata tactattcat ttctaagtta
    20341 tataacttac ctgtagttgg cataaattgc cataaccctg cggctcccat acgcgaagta
    20401 gctctaggat tcagtgctga ttctactata gacaaatact ttagttcatt gggaagattg
    20461 tactgctcaa aaatagtttc aaaaattggg aaataatgct gactaagtgc aagcatataa
    20521 gatatttctc tgtttctgtc gcaatataaa ttgatataac gacctactac atcattatac
    20581 gtcatttcca aaattgtggg catcattgca atccgtttaa tacgaattga atcaggcgaa
    20641 taaggtaatg gagcgtaacg gaatgcacta tcacatgttt cttttgtttt aattcgcata
    20701 cgccatgcaa cacacatctc ttctattgct gtatcgaacg tttgcataga tgattcatac
    20761 tcagaaacaa gtaacggaga gctctgcgat ggataaatag aagaggcata gcttggtgta
    20821 acaaacacca accatataac gaacaaactg gatataattt tatttataaa cattctttct
    20881 actacctctt aattatcaaa aagaaaattg acaactaagc ccaaaagaaa catcactaaa
    20941 atgattgata tgtttcaatg gaatggcttt aggtactatt tttaaagata aatctggact
    21001 aacatcaaaa tcatataaat gagcatcaac atacgcatct actatactta aaagatatag
    21061 accgaccgtt ccaataatac tcaaatccct gtatcttaaa taattatcct gtttattttt
    21121 caacaaactg gtatagtatg ctttgttatc actatctatc actactccct gaggtagtat
    21181 gtttacataa ctttgggtac tagcatcatt atcaacaata tctctatatg ctttttgata
    21241 atccctatac attcctccat tccacgaaat agcatacgtt aaacccattg cacctccata
    21301 caatataggc agtttccaat attttctgtt gtatatctgt cctagcccag gaactatagc
    21361 tgcataccaa atagcttttg aaggttgggg tttaaactca gcgtgtaaaa gtgttgaatc
    21421 aacatataac actacctctt tttcttgtac tgagtctgat tctgatacta caaccaaaga
    21481 atcatcactt gcccacaaag atagacaact aaatacgaat atagcaaata tgtaatattt
    21541 cagtttcaaa agtttaacta attacatcat tttatcaaaa acatccatca aaccttctaa
    21601 ttcttcatcc gaccgaaaag gtaaaataat tttcccttta ccgctttcat tacgttctat
    21661 tttaacagaa acatgtaaaa aatcagacaa tcgcttactc aatgcagtat actcagcagg
    21721 taatcgagga gttgattttg taactttttg ttccccttct acttcaagac tttgaaggtt
    21781 ctttactaat tcttctactt ttcgaacaga aagaccctct ttcacaattt tttcatatac
    21841 ttgcaacatc acacttgcat cagaaatcgt aaccaatgca cgtgcatgcc ccatatcaat
    21901 ttttttattt gtaataccca gttgaatctc agcaggtaat ttaagcaaac gaagataatt
    21961 agctattgta gaacgttttt tgcctacacg ctcgctcatg gtttcttggg tataaccaga
    22021 aatctctatc aatttttgat aactcaatgc aatttctatg gcatttaaat cttcacgctg
    22081 aatattttca accagagcca tttccattac agcctcgtca tctacggttt taacgtaagc
    22141 aggaattttt gttaaaccag ctatttttga agcacgataa cgtctttctc cagcaataat
    22201 ttgatacgta tcgctagaca cttgacgcaa agtaattggc tgaatgatac caacttcttt
    22261 aattgatgtt gctaattctt gcaaagcttc ttcatcaaaa taagtacgtg gttggtctgg
    22321 attggcttgt atcttagtta tatccacctc gcttatcgag gatgaaccac tagtagctac
    22381 cgtttccatt tcgatgagag cacccaagcc tctgcctaaa cctgattttt tattcattgt
    22441 acgggattat aacttaatat attatgaatt atttttaata atctcagccg ccaattgcat
    22501 atggttttga gctcctttag aatctgcatc atacaaaact acaggcaagc catgactagg
    22561 cgattcactc aagcgtatgt tacgttggat aactgtttca aacaccatat cttgaaaatg
    22621 ttttttaacc tcagcatata cttgattggc taatcgcaaa cgagaatcgt acatggttac
    22681 taaaaagcct tcaatatcaa gcgatggatt tagccgactt ttgataattt ttacggtatt
    22741 taacagcttg cttattcctt caagcgcata atattcagct tgtacaggta taattaggga
    22801 atctgctgct gtaagcgaat tgactgtaat caagcctagc gaaggagaac aatcaattaa
    22861 gataaaatca tactcatttt ttatcgtttc taacatggta cgtaaaaact tttcacgatt
    22921 atcgagtttc aacatctcaa tctcagcacc gaccaaatca atatgcgagg gcaaaatatg
    22981 caagccttcg atgggtgttt tttggatggc tttgcttacg ggcacattat ttatcaaaca
    23041 ttcataaata ctgtattcta atttttcaat atcaatacct aagcccgaag aggcatttgc
    23101 ttgtggatct gcatctacaa tcaacacttt cttttcgagt accgccaacg aagctcctaa
    23161 atttactgtg gttgttgtct ttcctactcc acctttttga tttgctaagg caataatttt
    23221 acccatattg tatttaaaat ttttacgttt tttccaaacg caaatgtagc aaaaattttg
    23281 aggttgaatg tttatttttt caacattttt agacttacat ctatttttat agtacttttg
    23341 caccatgaaa ataagcctca tcatatctat atacaagaat gtaccttttt tgaaactggt
    23401 gcttgaatcc ttgcaagttc aatcatatca ggattttgaa attattatat cagaagatgg
    23461 agaagacgaa aatgtgcgtc aatttgtagc ctcataccca tttaaacaaa gttatcaaca
    23521 tcttacacaa aaagatgatg gatggcaaaa aaataaggca ctgaacaatg caataagaaa
    23581 atctcagggt gattggctca tttttattga tggtgactgt gtgcttcaca aacgatttgt
    23641 tgaatttcat gcaaaaatgg ctgacgagca cctaatttta gctggcaaac gtgttaaatt
    23701 atcggcagaa accagtaaca aactgctttc aaacccaacc ttattgccaa ccttatcgcg
    23761 ttatttatat atccactttt ggaaatctgg tcgatatttg gaagaagctt tatttattca
    23821 tccacagggt ctgttgggtt ggataacacg tatacgcacg atgaaacatt taaaaggatg
    23881 caacatgtct ttttcaaaaa aagcactata tgcaattaat ggatttgatg aagactatac
    23941 cctacctgca attggcgaag atgtggatat tttatggcgt ttacaacaag caggctacca
    24001 actgaaatca gtacgcaata tggctgttat gtaccaccta catcacaaag aaagttgggt
    24061 aagtcaagaa gaaaacaaag aaaaaatgag gcaaaaaatg gaacaaaata cctatatttg
    24121 caaaaacgga atacaaaaaa tctaactata cagaacgcat gctatccatt attatttgtt
    24181 caaaatttgc cgaattatct tctattttaa cagccaatat tcacgaaacc gtaggtgttg
    24241 actatgagat tatccatata gacaattcac aatcaacaca ttctattttt tctgcataca
    24301 atctgggagt aacaaaaagc aaattccctt acttatgttt tgtacacgaa gatgtttttt
    24361 ttagaagctc tgattgggga cttaacatca tagaacattt acaaacgcca caagttggct
    24421 taataggctt agcaggtggt tgtttagctt cgcgcatacc agcgtcatgg tcaacacatg
    24481 gtttaaagat gaacctaatt caatcaggca aaggaaaatc tagttatatt cgcactccca
    24541 accaacccgc atcaacccgt gaagaagttg ttctgttaga tggtgtcttt ttagctacga
    24601 caaaagaaaa ctttcaattt ttgcaatttg atgaaaaaat ttcgggcttt catggatatg
    24661 attacgatat ttgtatgcaa gctaaagcca ataatttaag aaattatgtt gtttacgata
    24721 ttttactaga gcatttttct gcaggctctc acaatacgga ttattacaaa aatttgctcc
    24781 ttgtttataa aaaatggata aaccaacttc cattttcttg cacacaccta tcagaagcag
    24841 aaaccaagtt gatagaaaaa aagaaattaa agaaattgat tcgcaaatta ggtattcgta
    24901 attttactta ctcggaaata aaacaagaac ttgattattt tgccactatt accgataatc
    24961 agaaagtact tacatggttt ggttttaaat tattttacgc acgattattc aacaatcctt
    25021 cccgtttatt caaataacgc aacttatgtt agaaaaattt aggcattacg tacatagtat
    25081 caatctgctt tgtggacttt ttttaatggc ttcgttgccg ttttattttc caaaacaaca
    25141 gttagccctt acgttgtttt ttatatccta tatcgtagaa tttttggttg atatgaagtg
    25201 gagacagata caaatttcgg ctaccacctg gtattttatt ggtatattgt tgttttttac
    25261 aatgggtttt ttgtattatc cattcgaaga ttcgcatacc tattttcaaa acctaacaga
    25321 acgtcgatta gccttatttg gatttgctat aataggtatt tttggatata attcgttgta
    25381 taaattatcc tattttttac atactatcat tatttcatct accttgctta tcctttatct
    25441 tattatttac cacattggct tgcccgaatt tttgacaaac gaaaataggt atgatttatt
    25501 tacgcaagct cgcattgcat acgttaactc gcatatggct tttaacttat acttaaatat
    25561 tgcattggct agtgtctggt atttggctgt aacacgcttt aaagaactcc attggagtgt
    25621 gaaaactata tatggcttta gtattttgct tattataagc accttattaa tgtcggaggg
    25681 tcgttctgga tttatcgctg ctattggttt atccgctatc attgttattc gaaaaatttg
    25741 gttaacaaaa aaaatagtgg caattggagc ttccgttttt attgttattg gcatggtgtt
    25801 tgccatgttg caacacgaac gtatgtcaga aaaaatgatt gagcaagagc cacgcttatt
    25861 tttatggaca gaagttgccc tacccatcat acaagaaaaa ccgatattgg ggcatggatt
    25921 aagcgatgga cagacacaaa ttgacagtgt tcgacaagga aaacaatcta aaattttata
    25981 tgaattatgg aaaacaaata aacgcgtaga tgtacacaat cagtttttgc aaaccaccat
    26041 cgaatttggc ttgattggac ttgtcttgct cttagccatt tattactacc ctattaggct
    26101 ggttaatcgt agcagaatga ctttattgct attaggattg ttcctatttt cttggcaatc
    26161 gttgttcgac acattcataa cagggcaatt tgccattata tttggttttt ttataacgct
    26221 attgctaaca gtccccaaaa gtgaatcgct caataataac caaacagcgt taaaaaacct
    26281 ttggtaatac gggtatgcca tttattaaac ttattacgat tgcccaaggt ttggtacgtt
    26341 ccatcttgct tatcgtacac tagttcattt ctatttctca aataatcttt ccaatcgttc
    26401 cctttttcgt ggcgaaaaga accgcagcca tttgctttgg cttgctgacg cacagcatag
    26461 cgttgctgca tctgttgcgg actacggaac tgataatgca acaccttaat ttgtttcgga
    26521 taagttaatc cacaaggttc tggtctgcgt tcgcctgttt cccactgcaa ttttttgcta
    26581 tgacgcaaaa aacgtagttc tgcccatgtg taaggttggt aatagcgaat cttgtgttta
    26641 tcttcttcaa aatcacctgt aaacgtatac tcttctacat cttcatgtgt aaggcaataa
    26701 tcaatacttg ctttgcata
//
LOCUS       DAOSRP010000009        26442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2039_length_26442_cov_4.998181, whole genome shotgun sequence.
ACCESSION   DAOSRP010000009 DAOSRP010000000
VERSION     DAOSRP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26442
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_26442_cov_4.998181"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLJ82_01220"
     CDS             <1..213
                     /locus_tag="PLJ82_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444324.1"
                     /note="catalyzes the interconversion of
                     tetrahydrodipicolinate and L,L-diaminopimelate in lysine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOR40163.1"
                     /translation="INSPHIWIKTPKNTPSWKFFQQLLYESHIVCTPGAGFGQFGEGY
                     FRFSGFNTRENTIEAMERMQVWRNRI"
     gene            342..2726
                     /locus_tag="PLJ82_01225"
     CDS             342..2726
                     /locus_tag="PLJ82_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40164.1"
                     /translation="MKSKLSGIIIVSMLLCINNYVQADNVQIRGEVVDAFSGHAIIGA
                     TVIIENSNPIIGCISDYDGNFVLSNLQPGRYTINVSSVGYHPASIHNMMAISGKDNVL
                     VFKLEEKINHLKDIIVTANNNKFDAINEFAFTSARSFTVEETERFAGSWNDPSRMVSN
                     FAGVMSSNDARNDIIIRGNSPSGLLWNIDGVNVVNPNHFGSQGTTGGPISMLNSNLLA
                     NSDFITGAFPSEYGNALSGVFDLNLRSGNRERFEFMGQIGLNGFEGLVEGPLKLGKNL
                     QNGSFIVDYRYSTLKVLRDIGFDFGTGDAIPKYQDLAVLIDLPTKNSGRFKFTGITGK
                     SNIEIGRNFDEQEALGKGPMNSASDFSGNVLIGILSHTYMCNEKTRIKSSISFQESGN
                     EIINDTIDYINKTYFNYYAANQNEKKVTASFQIKHRVNVQNNFTVGLIGNQLITSYHD
                     SVWVNEYAIRKYLRNIDKKNSSLVQAYATWQHKFSDNLIVNSGLHSQYYDLNQQLSFE
                     PRISGLWEFSPNQQISFGYGLHSQLQPRIIYFTKEYDYTTNMYGNDNQHIGFSRAHHY
                     VMGYDIRIGRNFRIKTEGYYQNLFDIPISSQNVQYSMLNTGSSYYYEPVYGLENSGKG
                     RNYGVELTVEQFMHKGLYFLLTGSLFDSKYKAYDQIWRNTAFNTNYASNLLAGYEMRV
                     GKKHFLTFDIRTVWSGGLRYTPIDLNMSILTGEEVISVDKTNEEQFPDYIRCDFRTGF
                     KYNSKRITQEIGIDLNNVTNHQNIFSQHYNKATQQISTVYQQGFMLILFYRINL"
     gene            2788..3825
                     /locus_tag="PLJ82_01230"
     CDS             2788..3825
                     /locus_tag="PLJ82_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOR40165.1"
                     /translation="MEPNRKGFLSHFIIYSRWYTVLILTSIISIIVGFVISYFLDFRL
                     TGWHYYLRVLATTLVLWIGCSTIVIYTWKKYPWILMPFKHLMMEVIGVFCLLTLYFGF
                     TLLIYMYSHVGISLIQSIEIHSIAFIIITFTTFFILAIHEAILFYNQWHEHYSKSIRL
                     EKDTIEAQYNLLKTQINPHFLFNSLTSLMSMVDGNPKAEKYIQTLSDYLRYMLADNIN
                     ELITLKDEVNNVTKFFYLQKIRFENNIHLKIRIDDVALNKKIVPLVLQILLENCIKHN
                     IIANKMPLYIEIYNDENYVSIKNNLQIKTSKQSIGKGIKNIEGRYQFLSANAIKISKT
                     NETFTVSVPLL"
     gene            3837..4595
                     /locus_tag="PLJ82_01235"
     CDS             3837..4595
                     /locus_tag="PLJ82_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOR40166.1"
                     /translation="MLKILIIEDEKPALALLEKMLAQIINNFTIIGRCDSIESSVTFL
                     KKHQDIDLIFMDIHLGDGNSFDIFLQIQIDTPIIFTTAFDMYAIKAFELNSIDYLLKP
                     IKKEDLTRSIEKFYKSKKHLQQFDIQYLSSIISNRSNIYKERFLVYIGNNIKAIMTSD
                     IAYFYSLDSATYLVTHNGNTYDVNMSLEQLIGVLDPQIFFRINRQYIVAFSAIEKIQL
                     LSTYRIKIVLLPPTDKEILVSSNKNQLFKEWLNR"
     gene            4692..6710
                     /gene="uvrB"
                     /locus_tag="PLJ82_01240"
     CDS             4692..6710
                     /gene="uvrB"
                     /locus_tag="PLJ82_01240"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR40167.1"
                     /translation="MDFILVSPYKPTGDQPQAIEQLASGILGGISDQTLLGVTGSGKT
                     FTIANVIEQVNKPTLVLSHNKTLAAQLYREFKSFFPNNAVEYFVSYYDYYQPEAYLPT
                     TDTYIEKDLAINDEIERLRLSTTSSLLSGRQDIIVVSSVSCIYGIGNPDDFTNNVINI
                     KQGEQIVRDVFLRQLVSSMYNRNEIDFQRGNFRVKGDTVDIWLAYADIMLRVIFWGNE
                     IESITTHDPLHLFTLEHHDAYKIFPATIFVTTKDSIHRAIGEIEIDLGKHVDYMKSIG
                     KHYEAKRLYERVTYDLEMIRELGYCSGIENYSRYFDGREPGSPPFCLLDFFPKDFLMV
                     IDESHVTLPQIRAMYGGDAARKKNLVEYGFRLPAAMDNRPLTFDEFNEHKKQTIYVSA
                     TPADYELERCNGIVVEQVIRPTGLLDPIIEVRPSLNQIDDLQEEIVQRVQKQERVLIT
                     TLTKRMAEELHLYLQRAGVRCTYIHSDVDTLERVEILDHLRSGFYDVLIGVNLLREGL
                     DLPQVSLVAILDADKEGFLRSHRSLTQTAGRAARNLNGKVIMYADKITESMRKTIDET
                     NRRREKQLAYNAEHNITPTAIAQHAHATLKNKELYTDHPTYSMDNSMVDVAADPIVQY
                     MNKTQLQKSIEKTKKQMQEASKNMEFLEAAQYRNELAKLQEILKSKNS"
     gene            complement(6707..7492)
                     /locus_tag="PLJ82_01245"
     CDS             complement(6707..7492)
                     /locus_tag="PLJ82_01245"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOR40168.1"
                     /translation="MLGKRNVRFLSILFLGILFVSQVYSQTITYDETEDTDVGLRLSA
                     EISKRLSRNWSISLEEELRLNDNISRFDRLYSSVSVGYTINPYINTSIGYQHRLIQQK
                     GKASTNYQGYLDFRHRGILSVSFSYPVGQFEFSLRQRLNGTLRTDSVNPLEKSNPQII
                     SRTRMQAEYSFWSIPLKPYVSVEMFNPLNNPAYISDVWLETMRYTGGFTYRLDARNRI
                     ELYYFYEDEMGKDVNIGRNSGRVTITTEKEHNHVLGIAYQYRF"
     gene            complement(7482..8195)
                     /locus_tag="PLJ82_01250"
     CDS             complement(7482..8195)
                     /locus_tag="PLJ82_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOR40169.1"
                     /translation="MTDLTTFDPSIAAEFGENSTIDFLGVALFDATSLLHLLMRFVFN
                     MLVCWVIVQFFYYKKSKRRDYYVTFIMFSTTMFLLIFLMKNVDLHIGLTLGIFAIFGM
                     MRYRTETVPIREMTYLFVITGISVINGLSMTVSYAELVATNLLFVILAYIIENQKVLK
                     HTSSKLVLYEKIDLIVPEKREELKADLEKRTGLKIEKLEIGHIDFLRDVAFVKIYYVL
                     SPGESNSIDSIGKINQYVG"
     gene            complement(8192..8983)
                     /locus_tag="PLJ82_01255"
     CDS             complement(8192..8983)
                     /locus_tag="PLJ82_01255"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOR40170.1"
                     /translation="MTRDELSKLAVLRAMDTISLSEMKAVKLMNRVDTKYIASVDALM
                     EILRRATDDYRVQVVEGVPISSYDTIYFDTIDLEMYHRHHNRVLCRKKVRMRSYLDTG
                     DCFFEIKHKSNKGRVNKKRIQLPISNFDSTSFNNESIEFLEKQTDYQGPDLLPQLRTT
                     FDRITLVNKEKTERLTIDINLNFENRQTGIIYGFPDIMIIELKQDSSSYSMMKSILMD
                     LRIKPQRISKYCLGTVLTNPAAKYNWFKKKLMYIDKIRQQQTFTL"
     gene            complement(8998..9420)
                     /locus_tag="PLJ82_01260"
     CDS             complement(8998..9420)
                     /locus_tag="PLJ82_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40171.1"
                     /translation="MKRILLIFLITLMSGAYIYALVTPETLAKREKRKHLTVKEWNTD
                     AKGKTSWLDNLTIYDDQGRKVEEIEYANYGQKERITYQYDNATGKVEREIVYDYRNKP
                     TRIRKYEYNNDGTKKKQYNYLPNGKLFSIKVFEYIFTD"
     gene            complement(9538..11034)
                     /locus_tag="PLJ82_01265"
     CDS             complement(9538..11034)
                     /locus_tag="PLJ82_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOR40172.1"
                     /translation="MANIKSLAKETAIYGVSSILGKFLNWLLVPLYTYVLESSADYGV
                     VTNLYSWTALLLVVLTYGMETGYFRFANKNKENADQVYSTTLVSVGSTSLLFALFCVF
                     FSQQIANFLGYPTHPEYVWMLLGVVVALDAFSAIPFAYLRYINRPIKFAALKFLYIVL
                     NIFFNLFFLIVCPWLLKEAPWTVSWFYNPHYGVGYVFVANLLSTIIQTLVLLPYVFAV
                     KFDFDMQLLKKILRYSIPLLLLGIAGIMNQTLDKILFPFLRTGVEGAAELGIYGATSK
                     IALVMLMFTQAFRLAYEPFIFAQQKDKNSLTTYALAMKFFIIFSLLIFLGMVLYIDIF
                     KYIIQQNYWEGLNVVPIVLFSFIFQGIFFNLSLWYKLTDKTIYGAWFSFLGTVIIVVG
                     NIVLVPLFSYTGSAWAAFLCYFVIMLVSYCFGQKYMPIKYDLKSIAIYSVLAICLYVI
                     SLFINTEYSWLDYLLKTVLLAIFVAFAIKRDMPLTSIPILGDWYAKKK"
     gene            complement(11063..12085)
                     /gene="ruvB"
                     /locus_tag="PLJ82_01270"
     CDS             complement(11063..12085)
                     /gene="ruvB"
                     /locus_tag="PLJ82_01270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR40173.1"
                     /translation="MEDNFDIRKGALTDKEQDVDIALRPLSFNDFSGQKSIVDNLKIF
                     VQAARMRGESLDHVLLHGPPGLGKTTLSTIIANELQVGFKITSGPVLDKPGDLAGLLT
                     SLETNDVLFIDEIHRLSPIVEEYLYSAMEDYRIDIMIDKGPSARSIQLDLNKFTLVGA
                     TTRSGLLTSPLRARFGINCHLEYYDNDVLINIIKRSAAILHTPCTQDAAVEIASRSRG
                     TPRIANALLRRVRDFAQVKGNGKMDREIACYALEALNIDKYGLDEIDNKILTTIIDKF
                     NGGPVGLTTISTALGEDAGTLEEVYEPFLIKEGFLKRTPRGREVTQLAYEHLGRIRKA
                     DIGSLF"
     gene            complement(12111..13004)
                     /gene="gldA"
                     /locus_tag="PLJ82_01275"
     CDS             complement(12111..13004)
                     /gene="gldA"
                     /locus_tag="PLJ82_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOR40174.1"
                     /translation="MSLYIDSLSKFYGTQHVLKNISFHVESGEIIGFLGPNGAGKSTA
                     MKIITGFVQPDSGSIFVDGIDVLNDTLHAQKIIGYLPEHNPLYLDMYVAEYLLFIAQL
                     YKLGKESKQRVEDIIEKTGLTREYTKKIGQLSKGYRQRVGLAQAMIHNPKVLILDEPT
                     TGLDPNQLVEIRELIKEVGKDKTVILSTHIMQEVQAVCDRYVIIREGSIVADSKKDKI
                     HDTMTIVVEFANPISKDQLFMIDKTTAIHAINARSYVVECNEDIRYLLYQFAVEQGVG
                     LVTLHLQEQNLEELFRKLTRN"
     gene            13177..14220
                     /gene="thiL"
                     /locus_tag="PLJ82_01280"
     CDS             13177..14220
                     /gene="thiL"
                     /locus_tag="PLJ82_01280"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOR40175.1"
                     /translation="METRTTISELGEFGLIERLASTISLQHPTSIKGIGDDAAVIENN
                     EGNSQTLISTDLLLEGIHFDLTYFPLKHLGYKAAIVNFSDIYAMNGTPQQITVSIGVS
                     NRFCIEDIEELYEGIKLACSTYKVDIIGGDTSASLTGLTISITCIGVVNTKTIAYRNT
                     AKEGDLICVSGNLGAAYMGLQLLEREKTLFRQTNNAQPDFTGKEYILQRQLKPEARKD
                     IIELLAKESIIPTSMIDISDGLSSETLHICTQSKVGCRIYEDKIPIDYQTASMANEFN
                     MNLVTAALNGGEDYELLFTVPLSMHEKIATLEGISIIGHTTKESLGCNLITRDGQEFQ
                     LKAQGWNALHKND"
     gene            14355..14930
                     /locus_tag="PLJ82_01285"
     CDS             14355..14930
                     /locus_tag="PLJ82_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOR40176.1"
                     /translation="MKTFELSGVTRTDLGKKPSKAVRNDNAIPCVMYGTGSNTHFTVT
                     ESDVRKLIYTPEIFEVHLTVDGKKSVAVMKDLQFHPVTDKVLHIDFLEVDNKKPIVLS
                     VPVKVEGLAEGVKAGGKLSLELRKLKVKALCKDIPENLVVNVEKLGLGKTLKAGQLSY
                     PNLEILNNKDNVIVAVKLTRAARGLAAKGGK"
     gene            14937..15500
                     /gene="pth"
                     /locus_tag="PLJ82_01290"
     CDS             14937..15500
                     /gene="pth"
                     /locus_tag="PLJ82_01290"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR40177.1"
                     /translation="MKYLIVGLGNIGSEYQDTRHNIGFTLLDAFAKASNVFFTEKRYG
                     AVCECKIKNKTMILLKPSTFMNLSGNAVRYWMKEEKIPIENVLILVDDLALPFGVLRL
                     KSKGSDAGHNGLKNIQEILGTDQYPRLRFGIGNNYPKGQQINYVLGKWTLEENTALPS
                     RIQIAIDIIQSFVLAGIQHTMNLYNNK"
     gene            15539..15934
                     /locus_tag="PLJ82_01295"
     CDS             15539..15934
                     /locus_tag="PLJ82_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOR40178.1"
                     /translation="MIKEEVRIDKWLWAVRLFKTRTLAAEACKKGKITIDTVSVKPSR
                     TIRVNDVIQIKKPPITYAFKVLAVTENRMGAKLVPSFMENVTSEEQYAILELSKISGF
                     IDRDKGTGRPTKKDRRSLESFLDTDWTEE"
     gene            15949..16479
                     /locus_tag="PLJ82_01300"
     CDS             15949..16479
                     /locus_tag="PLJ82_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOR40179.1"
                     /translation="MIIARQKRKENIAEYILYMWHIEDIIRAYKLDIEAIHTHIIMHF
                     SLDEATRKEMKEWYESLIEMMNKEQVMHTGHLQINQSSVRLLCDFHVELLENEDEKQY
                     HSLYHQTLPLIEEFRHKNALSDVPSIEVCLQFMYGIWLLKIQKKEISTETNQAVVQIG
                     KLLAFLSKKFKNNEEN"
     gene            16540..17409
                     /gene="rfbA"
                     /locus_tag="PLJ82_01305"
     CDS             16540..17409
                     /gene="rfbA"
                     /locus_tag="PLJ82_01305"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOR40180.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPISVLMLA
                     GIREILIISTPQDIHLYENLLGDGTSLGVKFTYAIQPSPDGLAQAFIIGDEFIGNDSV
                     CMVLGDNIFYGYNFSKQLKEAATLEDGAVVFGYYVNDPERYGVAEFDTNGKVLSLEEK
                     PAIPKSNYAVTGLYFYSNDVIQKAKGLKPSARGELEITDLNKLYLQEERLKVQLLGRG
                     MAWLDTGTHDSLLEASNFIATIENRQGLKVACLEEIAFNNGFINKDQLLKLAEPLKKN
                     YYGQYLLKIASQK"
     gene            17439..17993
                     /gene="rfbC"
                     /locus_tag="PLJ82_01310"
     CDS             17439..17993
                     /gene="rfbC"
                     /locus_tag="PLJ82_01310"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOR40181.1"
                     /translation="MEIIETKIPGLYIVQPKVFGDHRGYFCETYNEKTFHSLGITNKF
                     CQDNLSKSSYGVIRGLHFQLHPHSQAKLVSAVVGTVYDVAVDMRVGSPTYGEWVGIEL
                     SEENKTQFLIPQGFAHGFSVLSETAIFSYKCDNLYTPSAEGGILYNDPTLQIDWKVPF
                     EKQLVSDKDTKHPTLKDVKTNFVF"
     gene            18011..18892
                     /gene="rfbD"
                     /locus_tag="PLJ82_01315"
     CDS             18011..18892
                     /gene="rfbD"
                     /locus_tag="PLJ82_01315"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOR40182.1"
                     /translation="MTNVLITGAYGQLGSELKKLAPQYTQFTYFFTDVDTLDICNKQA
                     ILQFCIDNSIHFILNCAAYTAVDKAEEKEQHSLCYAINAEAVKNLGEVASQIGANIIH
                     ISTDYVFDGKAYLPYTEDITTNPQSLYGKSKLLGEQWLMKSCAHALIIRTSWLYSTFG
                     NNFVKTMIKLGAERTELKVIFDQIGTPTYAADLANTMLTIVAKSTEKATNYVPGIYNF
                     SNEGVCSWYDFTIAIHALYGISTCNVLPIESKEYPTKAPRPHYSVLNKQKIKTTFDIT
                     IPHWQTSLANCIGKLKT"
     gene            19051..20568
                     /gene="atpD"
                     /locus_tag="PLJ82_01320"
     CDS             19051..20568
                     /gene="atpD"
                     /locus_tag="PLJ82_01320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOR40183.1"
                     /translation="MSVKTARAYITQVIGPIVDIYFEEGGYALPEIHDALEIIKPSAK
                     NIIVECHQHIGERSVRTISMDSTDGLVRGMEVIPKNKPICMPIGNQIKGRLLNVIGDA
                     IDGIGELDRKGESPIHRDPPKYEDLTTTKEVLYTGIKVVDLLAPYSRGGKIGLFGGAG
                     VGKTVLIMELIHNIAKGYNGYSVFTGVGERTREGNDLLREMIESGVINYGEEFKKSME
                     EGDWDLSKIDHEALKTSMATLVFGQMNEPPGARASVALSGLTVAESFRDADASNAQGK
                     DILFFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGLLQERITSTKTGAITSV
                     QAIYVPADDLTDPAPATTFSHLDATTVLSRKIAELGIYPAVDPLDSTSRILEPNVVGE
                     LHYKTALRLVELLQRNKELQDIIAILGMEELSDEDKLIVHRSRRVQRFLSQPFHVAEQ
                     FTGIPGVFVPIEETIRGFNMIMDGEVDQYPESAFNLVGTIEDAIEKGKAILRGEN"
     gene            20575..20823
                     /locus_tag="PLJ82_01325"
     CDS             20575..20823
                     /locus_tag="PLJ82_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOR40184.1"
                     /translation="MIVEIYAPHKTLFSGNALAISLPGKMGKFAILDNHAPLVSVLGK
                     GNIYLTKKNEENISFSIDSGFVEVNNNIVKVCVEINKK"
     gene            20831..21205
                     /locus_tag="PLJ82_01330"
     CDS             20831..21205
                     /locus_tag="PLJ82_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40185.1"
                     /translation="MRKYYQIIVTSVIVSQLIIWLVFTFFLPEYWDTNFFLIPIYFLA
                     IEIVFCKLLERFYVAKQKLNWYVIFKLGKLLLSIILAALYILAIQTQAIAFLLKFGVV
                     YLVFLFVETWIFLDYQKAQKGK"
     gene            21202..22284
                     /gene="atpB"
                     /locus_tag="PLJ82_01335"
     CDS             21202..22284
                     /gene="atpB"
                     /locus_tag="PLJ82_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOR40186.1"
                     /translation="MNQYFKYILLCFTLLFVVSYTQAANNHKEDEKPFDANTFIFGHI
                     ADAYDFHITSVGHKHISVPLLVIVKGKENGWSVFPSYRITHGDTYKGYYIQKKGSYKG
                     KIVETLSNGEIHRPFDLSITKNVFSILLSIFILIALFVPAANAYKKNPLGVPTKKNHL
                     LEMVILSIQNDVVKPSVGKDYAKYSPYLLTVFFFILITNLMGLIPFFPFGANVTGNIA
                     VTMVLAIFTYLITNIFGSREYWKEIFWPDVPVMLKFPLPLMPLIELISTFTKPFALLV
                     RLFANIFAGHMIILVLMALIFIFGSINPGVGYGVSIISIGFSIFMFMLELLVAFIQAY
                     VFTTLSAIFIGLSRVEPHHHPKKEIN"
     gene            22316..22570
                     /gene="atpE"
                     /locus_tag="PLJ82_01340"
     CDS             22316..22570
                     /gene="atpE"
                     /locus_tag="PLJ82_01340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOR40187.1"
                     /translation="MLLSIILQAVASASLAKLGASLGAALAVVGAGLGIGKIGSSAME
                     AIARQPEAASDIRSSMIVSAALIEGATLFAIIVCALAILI"
     gene            22615..23115
                     /gene="atpF"
                     /locus_tag="PLJ82_01345"
     CDS             22615..23115
                     /gene="atpF"
                     /locus_tag="PLJ82_01345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOR40188.1"
                     /translation="MDLFLPESGLVIWMTIAFGIVFFILSKFGFPIILKSINEREKYI
                     IKSLDAANEAQQKLLEIEDKGKEIIANAEQQQLLIVKESQELKKKLVEEAKMQAQKEA
                     DNIIENARIQIEKEKETALSDIRNEVLQVSFSIAEKVLEKELENKKAHTQYVEKLLDN
                     MSMSQS"
     gene            23124..23660
                     /gene="atpH"
                     /locus_tag="PLJ82_01350"
     CDS             23124..23660
                     /gene="atpH"
                     /locus_tag="PLJ82_01350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOR40189.1"
                     /translation="MNEGKISVRYARAILNLAKNKGIEDAVYKEMFVLSDNLTHYAKD
                     FKTAFAHPDITEENKKKLFKLAVGNTISSFTSQSIDFIVEKKRSSFMLRIALMYQTLY
                     QIEHNIIISNVISAKELDKKTLDKIKAFASKTFDASVELVSTIDPDLIGGFILDIANQ
                     RLDASIKGKLNNIRKIVY"
     gene            23672..25255
                     /gene="atpA"
                     /locus_tag="PLJ82_01355"
     CDS             23672..25255
                     /gene="atpA"
                     /locus_tag="PLJ82_01355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOR40190.1"
                     /translation="MMASIKASEVSNLLQMELEGFSDKLKFEETGKVLQISDGVARVF
                     GLTKVQSNELVLFENGTKGVVLNLEEDNVGIVLLGSTRGIKEGDTVQRTGNIASILAG
                     EGLLGRVVNTLGEPIDGKGEIKGELFEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTEIAIDTIINQRSNFLAGDPIYCIYVGIGQKGSTIAGIVK
                     TFEEYGAMDYTIVVSATASDPAAMQFYAPYAGVAIAEYFRDTGRHALIIYDDLSKQAV
                     SYREVSLVLRRPPGREAYPGDIFYLHSRLLERAAKIIKNDVVARNMNDLPDSIKHLVK
                     GGGSITALPIIETREGDVSAFIPTNVISITDGQIFLETSLFNAGIRPAINVGISVSRV
                     GGNAQIKAMKKIAGTLKLDQAQYRELEAFSKFGADLDAGTKLVLNKGSKNVEILKQKL
                     HQPMPVEKQIAIILCGVRGYLSHVPLNKVQEFENNLLEILEAKYKKDVLDILQKGDLT
                     EDVLTKIKDVAFTLCKQYE"
     gene            25280..26158
                     /gene="atpG"
                     /locus_tag="PLJ82_01360"
     CDS             25280..26158
                     /gene="atpG"
                     /locus_tag="PLJ82_01360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOR40191.1"
                     /translation="MASLKDIRARILSVRSTKKITSAMKMVSSSKLHKTETLCLNFLP
                     YKNALCESLANYLNSLDEKISVQVAEQRPIKRMAIICFSSNSGLCGTFNTNIAKLASE
                     KIDQYAYLGKENIYVYTIGKKITDYLQKLKYPIHAHYNNLLDNPSYDEVVKLADMLTE
                     LFISKQIDCVELLYNHYKNTACQTPTSEILLPLSSMQLTRTTKSQPLYIVEPSKEEFI
                     NALAPKLIRIKLYAAFLDSFTAEHAARTTAMQIATENAEEMIKTITQQYNKKRQEVIT
                     NELLDIVGGSEALRQD"
     gene            26175..>26442
                     /locus_tag="PLJ82_01365"
     CDS             26175..>26442
                     /locus_tag="PLJ82_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR40192.1"
                     /translation="MEITRVFDILENYKIIKPNQPIALAHKKEGNWIKYSIDEYIYQT
                     NLISAGLLYNGVKKGDTIAIISSNRAEYNIVDMAAMQIGAIPVPI"
BASE COUNT         9305 a         4459 c         4437 g         8241 t
ORIGIN      
        1 attaattccc ctcatatttg gattaaaaca cccaaaaata caccatcatg gaaattcttc
       61 cagcaactgc tttacgaatc gcatattgtt tgcacaccag gagcaggatt tggtcagttt
      121 ggcgaagggt atttccgctt ttccggattc aatacccgcg aaaacaccat cgaagcaatg
      181 gaaagaatgc aggtgtggag aaataggata taacacgaaa gtatgttttc aatttcgatt
      241 tttcaatatt caatatcctt ttttgtacat acaactcttt ttatttttaa tcaataaaac
      301 cttaaaatac ctttgcgttc attaactatt ataaatgtac aatgaaaagc aaactatcgg
      361 gtattattat tgtgagtatg ttattgtgta taaacaatta tgtacaagcc gacaatgttc
      421 aaatcagagg agaagtagta gatgccttta gcggacatgc tattatagga gcaacagtga
      481 ttattgagaa ttctaatcca ataattggtt gtatttctga ttatgatggc aattttgtgt
      541 tatctaactt acaaccagga agatacacca ttaatgtatc ttctgtgggt tatcatcccg
      601 ccagcattca taatatgatg gcaattagtg gaaaagataa tgttcttgta tttaagcttg
      661 aagaaaaaat taatcacctt aaggatatta ttgttacagc caataataac aaatttgatg
      721 caattaatga atttgcattt acaagtgcac ggtcatttac tgtagaagaa actgaaagat
      781 ttgctggcag ttggaatgat ccgagtagaa tggtgtctaa ttttgcaggt gtaatgagca
      841 gtaatgatgc ccgtaatgat attatcataa gaggaaactc accaagcgga ttattatgga
      901 atatagatgg tgtaaatgtt gtaaatccta atcattttgg ttctcaagga actactggag
      961 gaccaataag tatgcttaac agcaatcttt tggcaaactc cgattttatt acgggtgctt
     1021 ttcctagtga atatggtaat gctttatcgg gagtgtttga tttaaatctg agatcaggaa
     1081 atagagaaag atttgaattt atgggacaaa ttggcttgaa tggttttgaa gggttagttg
     1141 aaggtccttt aaaacttgga aagaacttac aaaatggctc atttatagta gattatcgtt
     1201 attctacatt aaaagtttta cgcgatattg gtttcgattt tggcacaggt gatgccattc
     1261 cgaagtatca agatttagca gtattaattg acctcccaac aaaaaattct ggacgtttta
     1321 agtttacagg aattaccggg aaaagcaata tcgaaatagg aagaaacttt gatgaacaag
     1381 aagcattggg aaaaggtcct atgaattcag cgtcagattt ttctggaaat gttctgattg
     1441 gaattttatc acacacatat atgtgtaacg aaaaaacaag aattaaatct tctatatctt
     1501 ttcaggaaag tggcaatgaa attataaatg atacgataga ttacatcaac aaaacctatt
     1561 ttaattacta tgccgccaat cagaatgaga aaaaagttac tgcatctttt caaataaaac
     1621 acagggtaaa tgttcaaaat aattttacgg tagggttaat tggcaatcaa cttattacct
     1681 cataccatga ttctgtatgg gtaaatgagt atgcaatacg gaaatatcta agaaatattg
     1741 acaaaaaaaa tagttcactt gtgcaggctt atgcaacgtg gcaacacaag ttctctgaca
     1801 atttgattgt aaattctggt ttgcatagtc aatattacga cctcaaccaa cagctaagtt
     1861 ttgaacctcg aataagtgga ttatgggaat tttctcctaa ccaacaaatt agttttggat
     1921 atggattaca tagtcaattg caacccagaa ttatatattt cacaaaagaa tacgattata
     1981 ctacgaatat gtacggaaat gataatcaac atattggatt ttctcgtgct catcattatg
     2041 taatgggata tgatatacgc attggtagaa atttcagaat aaaaaccgag gggtattatc
     2101 aaaacttatt tgatattcca atttcttcgc aaaatgtaca atattcgatg cttaatacag
     2161 gtagtagcta ttattatgaa cccgtatatg gtttagaaaa ttcgggtaaa ggcagaaatt
     2221 atggtgtaga gttaactgta gaacagttta tgcacaaagg actatatttt ctacttacag
     2281 gttcgctgtt tgactcaaaa tataaagctt atgaccaaat atggcgtaat acagctttta
     2341 atacaaatta tgcaagcaat ttgttggctg gttatgaaat gcgtgttggt aaaaaacatt
     2401 ttcttacctt cgatattcgc actgtttggt cgggaggact gcgatatacg ccaattgact
     2461 taaacatgtc aatattaact ggtgaagaag taataagtgt tgacaaaacg aatgaagaac
     2521 aatttccaga ttacataaga tgcgattttc gaaccggatt taaatacaac tctaaacgaa
     2581 ttacgcaaga aattggcatt gatttaaata acgttacaaa ccaccaaaat atattttcac
     2641 aacattataa taaagctact caacaaattt caacagtata tcaacaagga tttatgctta
     2701 ttttgtttta tcgtattaat ttataagcac attttcaatt ggtagtgcca taaattttat
     2761 gtaatttcgc acaaaactac tatgaatatg gaacccaata gaaaaggttt tttatcccat
     2821 tttattatct attcaagatg gtacacggta ttgatattga caagtatcat tagtataatt
     2881 gttggttttg ttatttcata ttttttagat tttcgtctta ctggatggca ttattaccta
     2941 cgtgtattgg ctacaacctt ggtattgtgg attggatgtt ctactattgt aatatatact
     3001 tggaaaaaat acccatggat tttgatgcca tttaaacatt tgatgatgga ggtaataggc
     3061 gttttttgtc tattaacatt atactttgga tttacattat tgatttatat gtattcacat
     3121 gtaggtatta gtttaattca aagcattgaa atacattcca ttgcatttat tattataact
     3181 tttaccactt ttttcatttt ggctatacat gaagctatat tgttttacaa ccaatggcac
     3241 gaacattatt caaaatcaat tcgactagaa aaagatacta ttgaggctca atacaattta
     3301 ctcaaaactc aaattaatcc acatttttta ttcaatagcc ttactagcct aatgagcatg
     3361 gttgatggca accctaaagc cgaaaagtat atacaaactt tatctgatta tctgagatat
     3421 atgctggcag acaatataaa tgaattaata acactaaaag atgaggtgaa taacgtaact
     3481 aaattctttt atttacaaaa aatccgattt gaaaacaaca tccatcttaa aattaggatt
     3541 gatgatgtgg cattaaacaa aaaaattgta cctttggtat tacaaatatt gcttgaaaat
     3601 tgcattaaac ataatatcat tgcaaataaa atgcctttat atatagagat ttataatgat
     3661 gagaactatg taagcattaa aaataatttg caaattaaaa caagcaaaca atccattggt
     3721 aagggtatta aaaatattga aggacgctac caatttttat cagctaatgc cataaaaata
     3781 tcaaaaacaa atgaaacgtt tacagtatct gtgccattgt tataagaaat atgtgtatgt
     3841 tgaaaatatt aatcattgaa gatgaaaaac ccgccttggc attgttagaa aaaatgctgg
     3901 ctcaaataat caataatttt acaattattg gaagatgtga tagcattgag tcgagcgtaa
     3961 ctttcttgaa gaagcaccaa gatattgatt taatttttat ggatatacac ctgggagatg
     4021 gcaatagttt tgatatattc ttacaaatac agattgatac acctatcatt tttactactg
     4081 cttttgatat gtatgcaata aaagcattcg aactaaacag cattgattat ttgttgaaac
     4141 ccataaaaaa agaagattta actagatcaa ttgagaaatt ttataagtcg aaaaaacatt
     4201 tacaacaatt cgatattcaa tatttatctt ccattatttc taatcgttct aatatttata
     4261 aagaacggtt tttggtgtat atcggaaaca atataaaagc aattatgaca agtgatattg
     4321 cctattttta ttcacttgac agtgccacat accttgtaac acacaatggc aatacttatg
     4381 atgtaaacat gtcgttggaa caattgattg gtgttttaga cccacaaatc ttttttagaa
     4441 ttaacagaca atacattgtt gcatttagtg ctatcgaaaa gatacaatta ctttctacct
     4501 accgaattaa aatagtatta ttgccaccca cagataaaga aatacttgta agtagcaata
     4561 aaaatcagtt gtttaaagaa tggttaaata gatgaaaaat agtttttata actgaaaaaa
     4621 ttgttgtcta tctattctta tttttgcttg tatcaatata agtcattaca ttcgcgtaaa
     4681 taatcttatc tatggatttt atactcgttt ctccttataa accaactggc gaccaacctc
     4741 aagctatcga acaattagct agtggcattt tgggtggaat aagcgaccaa accttattag
     4801 gggttactgg ttcgggtaaa acatttacca tagccaatgt gattgagcaa gtaaataaac
     4861 ctactctcgt tttaagtcac aataaaactt tagctgctca attatacaga gaatttaaaa
     4921 gtttttttcc gaacaatgca gttgagtact ttgtttcgta ctacgattac taccagccag
     4981 aagcatactt accgaccact gatacctata tagaaaaaga tttagccata aatgatgaaa
     5041 tagagcgtct taggctctct actacttcat ctctactttc tggcagacag gatattattg
     5101 ttgtttcttc cgtttcgtgt atctatggta ttggtaatcc tgatgacttt acgaataatg
     5161 taattaacat caaacaagga gaacaaatag tgcgtgatgt gtttctcaga caattagtat
     5221 catccatgta taaccgaaat gaaattgact ttcagcgggg taattttagg gtaaaaggag
     5281 atactgtcga catttggctt gcgtatgctg acattatgct acgtgtcatt ttctggggaa
     5341 acgaaataga aagcattact acccacgacc cattgcactt attcacacta gagcatcacg
     5401 acgcttataa aatatttcct gctactattt ttgttactac caaagatagt attcatagag
     5461 ctattggcga aatagaaatt gatttgggaa aacatgttga ttatatgaaa tcaattggca
     5521 agcattacga agccaaacga ttatacgaac gtgtaactta cgacttagaa atgatacggg
     5581 agttaggcta ctgctctggc atcgaaaatt attctcgtta ttttgatggt agagaacctg
     5641 gttctcctcc gttttgtctg ttagatttct ttccaaaaga ctttttaatg gttatagacg
     5701 aaagtcacgt aacactccct caaatacgtg ccatgtatgg tggcgatgct gctcgcaaaa
     5761 agaaccttgt tgaatatggc tttcgattac ctgcagccat ggataacaga cctcttacat
     5821 ttgatgaatt taacgaacat aaaaaacaaa ctatttacgt aagtgctact cctgctgatt
     5881 acgaattaga gcgttgtaat ggtattgttg tagaacaagt aattcgccca actggtttgc
     5941 tcgaccccat catcgaagtg cgtcctagtt tgaatcaaat agacgattta caagaagaaa
     6001 ttgtacaacg tgtacaaaaa caagaacgtg tactaattac cactcttaca aaacgtatgg
     6061 cagaagaatt gcatctctac ctacaacgtg caggggtacg atgtacatac atacactctg
     6121 atgtggatac gcttgaaagg gtggaaatat tagatcactt acgttctgga ttttatgatg
     6181 tattaatagg tgtaaactta ctaagagagg ggcttgattt gccacaagta tcgttagtcg
     6241 ctattcttga tgctgataaa gaaggctttc ttcgttcgca tcgctcgctt acccaaacag
     6301 ctggtagagc cgcacgcaac ctcaacggaa aagtgattat gtatgctgac aaaataaccg
     6361 aaagcatgcg gaaaacaatc gacgaaacga acagacgaag agaaaaacaa cttgcgtaca
     6421 atgccgaaca taatattaca ccaactgcca tagctcaaca cgcacacgca acgttaaaaa
     6481 acaaagaatt atacacagac catccaacat actcaatgga caatagcatg gtagatgttg
     6541 ctgctgaccc aattgttcaa tacatgaaca agacacaact ccaaaaatct atagaaaaaa
     6601 caaaaaaaca gatgcaagaa gcctctaaaa atatggaatt cttagaggct gctcagtatc
     6661 gtaatgagtt agcaaaatta caagagattt tgaaatcaaa gaatagctaa aacctatatt
     6721 gataagcaat tccaagtaca tggttatgct ctttttcggt tgttatggtt actcgcccgc
     6781 tattgcgtcc tatattcaca tccttaccca tttcatcctc ataaaaataa tacaattcta
     6841 ttcggtttct cgcatccaag cgataagtaa aacctcctgt gtatcgcatt gtttctaacc
     6901 aaacgtcact aatataagca ggattgttca acggattaaa catttctaca gaaacatatg
     6961 gctttaaagg aatactccaa aaactgtact cagcctgcat tcgagtacga gaaatgattt
     7021 gtggatttga tttttctaag ggattaacag aatctgtacg taaggtaccg tttaatcgtt
     7081 gtcgcaaact gaattcaaat tgaccaactg gataggaaaa tgaaacactc aatattcctc
     7141 tgtgtctaaa atctaaatat ccttgataat tagtactcgc tttccctttt tgctgtatta
     7201 acctatgttg atagccaata ctcgtattaa tatatggatt aattgtataa cccacagata
     7261 cagatgaata taacctatcg aatcgactaa tgttatcatt caaccgtagc tcctcctcta
     7321 acgatattga ccaattacga cttaatcttt ttgatatttc agcagacaat ctcaacccca
     7381 catcagtgtc ttctgtttcg tcgtacgtta tagtctgaga atacacctgc gaaacaaaca
     7441 atatacctaa aaaaagtatg cttaaaaatc taacattgcg tttacccaac atattgattt
     7501 atttttccaa ttgaatcaat gctatttgat tcaccaggac ttaatacata atatattttt
     7561 acaaaagcca catcacgtaa aaaatcaata tgacctattt ctaatttttc aatctttaat
     7621 cctgttcgtt tttctaaatc ggcttttagt tcctctcttt tttcaggaac tattaaatca
     7681 attttttcgt acaacactaa ttttgacgat gtatgtttta atactttttg attctcaata
     7741 atatatgcaa gaataacaaa taataaattt gtagcaacca attcagcata gctgacagtc
     7801 atcgacaaac cattaataac cgaaatacct gtaataacaa ataaataggt catctctcgg
     7861 ataggaaccg tttcggttct atacctcatc atgccaaaaa tggcaaatat acctagtgtt
     7921 aatccaatat gtaaatcaac attcttcatt aaaaaaatca acaaaaacat ggtagtgcta
     7981 aacataataa acgtaacata ataatcacgt cttttgcttt tcttataata gaaaaactgc
     8041 actatcaccc aacatactaa catgttgaaa acaaaacgca ttagtaaatg caataaactg
     8101 gtagcatcaa atagagctac acctagaaaa tcaatggtag aattttcgcc aaattccgca
     8161 gcaatactgg gatcaaaagt tgttaaatct gtcataatgt gaaggtttgt tgttgtctaa
     8221 ttttatctat gtacattaat tttttcttaa accaattata tttggcagca gggttggtaa
     8281 gcactgtgcc taaacagtat ttactaatac gttgcggttt aatacgtaaa tccattaaaa
     8341 ttgatttcat catagaataa ctcgaactgt cttgtttcaa ctctataatc attatatcag
     8401 gaaaaccgta tattatacct gtttgcctat tctcaaaatt tagatttata tcaatagtta
     8461 aacgttctgt tttttcttta ttaacaagtg taattctatc aaacgtagta cgcaactgag
     8521 ggagtaaatc tggtccttga taatctgttt gtttttccaa aaattcaata gactcattat
     8581 taaaagaggt ggaatcaaaa tttgaaattg gcaattgaat gcgttttttg tttacacgtc
     8641 ctttgttgct tttatgttta atttcaaaaa aacaatcacc tgtatctaaa tagctacgca
     8701 tacgtacttt ttttcggcac aatacacgat tgtgatgacg atgatacatc tctaaatcaa
     8761 tggtatcaaa ataaatagta tcataactgc taatgggaac accttcaacg acctgcacgc
     8821 gataatcatc ggtagctcta cgtaaaatct ccattaacgc atcaacactc gctatgtatt
     8881 ttgtgtcaac acgattcatt aattttacgg ctttcatttc cgagagcgaa atggtatcca
     8941 ttgctctcaa aactgccaat tttgataatt cgtctctagt catttatata gatcaaatta
     9001 atcagtaaat atatattcaa atactttaat cgaaaataac tttccgtttg gcaaataatt
     9061 gtattgtttt ttctttgtgc catcattatt gtattcgtat ttacgtattc ttgtaggttt
     9121 attacgataa tcataaacaa tctcccgttc tactttacca gtagcattat cgtattgata
     9181 agttatacgt tccttttgtc cataattagc atactcaatc tcctctactt ttctcccttg
     9241 atcatcatat attgttaaat tatccaacca actggtttta cctttggcat ctgtattcca
     9301 ctcttttaca gttaaatgtt ttcgcttttc gcgtttggct agtgtttctg gtgttactaa
     9361 agcatatata tatgctccag acataagggt gataagaaaa attaatagaa ttcttttcat
     9421 aatacatgta taaggttgtt acaaacaaat atattaacta aaatggctaa caaatacagc
     9481 tacatttccg ttaagagaac gcaaagatat aattaaacat agaaacttca aattatttta
     9541 cttttttttt gcataccaat cgccaagaat gggtatagat gttaacggca tatctctttt
     9601 gatggcaaat gctacaaaaa ttgccaataa gacagttttc agcaaatagt ccaaccaact
     9661 atattccgta ttaataaata atgaaatgac atataagcaa atagctaata cagagtagat
     9721 ggctattgat tttaaatcat actttatagg catatatttt tgtccaaaac agtatgatac
     9781 caacataata acaaaatagc acaagaaagc agcccatgcc gatcctgtat agctaaaaag
     9841 aggtactaat acgatattcc ctactacaat gattactgtt cctaaaaaag agaaccatgc
     9901 tccgtaaatg gttttatccg taagtttata ccacaaagaa aggttgaaaa atattccctg
     9961 aaaaatgaag gagaacaaaa caataggaac aacgtttaac ccttcccaat aattttgttg
    10021 aatgatgtat ttaaaaatgt caatatataa caccattcca aggaaaatta gcaaggaaaa
    10081 aatgatgaaa aacttcatgg ctaaagcata tgttgtcaaa ctattcttat ctttctgttg
    10141 agcaaaaata aatggttcat acgcaagccg aaaagcctga gtaaacataa gcatcaccaa
    10201 ggctatctta gatgttgctc cataaattcc aagttcagca gctccttcta ctcctgtcct
    10261 cagaaatgga aaaagaattt tgtccaatgt ttgattcata atgcctgcga tacctaaaag
    10321 caacaatgga attgaatagc gtaagatttt tttcagcaat tgcatgtcaa aatcaaattt
    10381 taccgcaaaa acatatggta ataaaactaa cgtttgaata attgtagaca ataaatttgc
    10441 aacaaataca taaccaacac cataatgggg attataaaac cagcttactg tccacggggc
    10501 ttctttcagt aaccaaggac atacaatcag aaaaaaaaga ttgaagaaaa tattcaatac
    10561 aatatacaaa aacttgagtg ctgcaaattt tattggacgg ttgatgtatc gtaaataagc
    10621 aaacggtatt gcagaaaagg catctaatgc aacaacgact cctagcaaca tccaaacata
    10681 ttcaggatgt gtcggatagc caagaaaatt agctatttgc tgcgaaaaga atacacaaaa
    10741 taatgcaaat aataatgatg tactcccaac tgaaactaac gtagtactgt acacttgatc
    10801 ggcattttct ttgtttttgt tggcaaaacg aaaatatcca gtttccatac cgtatgtcaa
    10861 aacaactaac aacaaagctg tccaagaata caagtttgtt actacaccat aatcggcaga
    10921 actttccaga acataggtat aaagaggaac aagtaaccaa ttaaggaatt ttcctaaaat
    10981 gctacttact ccatatattg ctgtttcctt tgcaagactt tttatatttg ccatgaatac
    11041 gattattctg atgaataaaa acctaaaata aagaacctat atctgctttt ctaatacgac
    11101 ctagatgctc atatgccaat tgcgttacct ctctaccacg aggtgtgcgt ttcaaaaaac
    11161 cttcctttat tagaaacggt tcgtacacct cctctagagt tccagcatct tcgcccaaag
    11221 ctgtcgaaat ggtagtcaat ccgacaggtc ctccattaaa tttatcaata atagtagtca
    11281 aaattttgtt atcaatctca tctaaaccat acttatcaat atttaatgct tcgagggcat
    11341 aacaagcaat ttctctgtcc atttttccat tcccttttac ttgagcaaaa tctcttactc
    11401 tacgcaataa ggcattagca atacgtgggg tacctctact tctgctagct atctctacag
    11461 ctgcatcttg agtgcatggg gtgtgtaata tagctgccga acgctttatg atattgataa
    11521 gcacatcatt gtcgtaatat tctaaatgac aattgatgcc aaatcgggct cttaacggac
    11581 tagtaagcag tccactacga gtagttgctc ctacaagtgt aaatttgttt aaatctagtt
    11641 gtatagagcg agcacttgga cctttatcta tcattatatc aatacgataa tcctccattg
    11701 ctgaatataa atattcttct acaatcggac tcaaacgatg tatttcatct ataaacaaca
    11761 catcattcgt ttccaaactg gttagcaaac ctgctaaatc accaggttta tccaatacag
    11821 gtcctgaagt tattttaaac ccaacttgca actcattggc aattatagtt gataaagtgg
    11881 ttttacctaa tccaggaggt ccatgcaata atacgtggtc taacgattct ccacgcatac
    11941 gagctgcttg aacaaaaatt ttcaggttgt ccacaatgct tttttgtccg ctaaaatcat
    12001 taaaacttaa tggacgtaag gcaatatcaa catcttgttc tttatcggtt aaagctcctt
    12061 ttcttatatc gaaattatct tccatctatg tttattttag tctatatcaa ctaatttcgc
    12121 gttaattttc taaacagttc ttccaaattt tgctcctgta agtgcaaggt taccaagccc
    12181 accccttgtt ctacggcaaa ttgatacaac aaataccgga tatcttcatt acattcaacc
    12241 acatacgacc gtgcattaat tgcgtgtata gcagttgtct tatctatcat gaatagctgg
    12301 tctttggata ttggatttgc aaattccaca acgatggtca tagtatcatg aatcttatct
    12361 tttttgctat cggcaacaat tgatccttct cgtataataa catatctatc acacacagcc
    12421 tgcacttctt gcataatatg cgtgctaagt attactgttt tatctttgcc tacctcttta
    12481 ataagttcac gtatttcaac taattgatta ggatcaagac cagtagtagg ctcatccaaa
    12541 ataagcactt tgggattatg aatcatggct tgagctaaac ccactcgttg acgataccct
    12601 tttgataatt gtccaatttt ttttgtatat tctcgagtta accctgtctt ttctattata
    12661 tcctctactc tctgtttact ttctttacct aatttataaa gctgagcaat aaacaataag
    12721 tattcggcaa catacatatc caaatacaat ggattatgct ctggcaaata tccaattatt
    12781 ttttgtgcat gtaaagtatc attcaacaca tcaattccat caacaaatat actaccacta
    12841 tctggctgta caaagccagt aataatcttc atcgcagttg acttccctgc tccattcgga
    12901 cctagaaaac caataatttc accactttct acatgaaatg aaatattttt caatacatgc
    12961 tgggtaccat aaaacttaga taaactatca atatacagac tcatgcagaa taaggttcta
    13021 taaattttga gcaaaattaa acataaaaag ggagtttgta tcatatttaa ctacaaattt
    13081 atttttttta tcatttcaaa atagataaca gctgtagctc tttgacattg ggatgtaaaa
    13141 aaatatctat atctttgcaa ttcaagaaaa aaatgtatgg aaacacgaac aactatttca
    13201 gaattaggag aatttggctt aattgagcga ttagcaagta ccatttcact acaacatcca
    13261 acctctatta aaggtatagg agatgatgcc gcagtaatag aaaataatga gggtaattct
    13321 caaacactta tttcaaccga tttattatta gaaggtattc atttcgattt aacctatttt
    13381 cctttaaaac atttgggata caaggcggct attgttaact tttcggatat atatgccatg
    13441 aatggcactc ctcaacaaat tactgtttct attggtgtat ctaaccgttt ctgtattgaa
    13501 gatattgaag aattatatga ggggattaaa ctggcttgct ctacgtataa ggtagacatt
    13561 attggagggg atacttcagc ctctcttact ggactcacta ttagtattac ctgcattggt
    13621 gtagttaata caaaaactat tgcgtatcgt aatacagcaa aagagggtga tttaatttgt
    13681 gtgtcaggca atttgggtgc agcctacatg ggattgcaat tattagaacg tgaaaaaaca
    13741 cttttccgtc aaacgaataa tgcacagcct gattttactg gaaaagaata tattttacaa
    13801 cgacaattaa aacccgaagc ccgtaaagat atcatcgaat tacttgcaaa agaatccatt
    13861 attccgactt caatgattga catatctgat ggtttatcat ctgaaacatt gcatatatgt
    13921 acccaaagta aagttggatg tcgcatatac gaagacaaaa ttccaattga ttaccaaacg
    13981 gcatcaatgg ctaatgaatt caatatgaat ttggtaacag ctgctttaaa tggcggagaa
    14041 gattacgaat tattgtttac tgttccatta tccatgcacg aaaaaatagc aactttagaa
    14101 ggaattagca ttattggaca tactacaaaa gaatcgttag gctgtaattt aattacacga
    14161 gacggacaag aatttcaatt aaaagctcaa ggttggaatg cattacataa aaatgactaa
    14221 gtatctcttt tgtaaataaa aaaaaataca tacctttgca ccgcttttcg aaagaaagtg
    14281 tgatgggaat caagtcacta atcacttaat attataactg gacttgaatt tgagtaacac
    14341 aacaaaaaat taacatgaaa acatttgaat tatctggagt aacaagaaca gacttaggta
    14401 aaaaaccaag taaagcagta cgtaacgaca acgcaatacc ttgtgtaatg tacggaactg
    14461 gtagcaacac tcactttaca gtaaccgaaa gcgatgttcg taaattaatt tacacacccg
    14521 aaatttttga agtacactta actgtagatg ggaaaaaaag tgtagctgta atgaaagatt
    14581 tacaatttca ccctgttaca gacaaagttt tacacatcga ctttttagaa gttgacaaca
    14641 aaaaaccaat tgtattatct gtgcctgtaa aagttgaagg cttggcagaa ggtgtaaaag
    14701 cagggggtaa attatcattg gaattacgca agctaaaagt aaaagctctt tgcaaagaca
    14761 ttccagaaaa tttggttgta aatgttgaaa aattagggtt aggaaaaaca ttaaaagctg
    14821 gacagttgag ctatccaaac ctcgaaattt tgaacaacaa agacaacgta atagtagctg
    14881 taaaattaac aagagctgca agaggattag ctgcaaaagg cggaaaataa taacacatga
    14941 aatacttgat tgtaggattg ggtaacattg gtagcgaata ccaagatact cgccacaaca
    15001 taggatttac actattggat gccttcgcta aggcatccaa tgtttttttt accgaaaaac
    15061 ggtacggggc tgtgtgtgaa tgtaaaatca aaaacaaaac aatgatttta cttaaaccct
    15121 ctacttttat gaatttaagt ggaaatgcag ttcgttattg gatgaaagaa gaaaaaattc
    15181 cgatagaaaa tgtacttatc ttagtagatg atttagcttt accttttggg gtattgcgac
    15241 taaaatcgaa aggaagcgat gctggtcata acggattaaa gaatattcaa gaaattttag
    15301 gtaccgatca ataccctcgt ttacgatttg gaattggcaa caactacccc aaaggtcaac
    15361 aaatcaacta tgtgcttggc aaatggacgt tagaagaaaa cactgctctc ccctctcgca
    15421 tacaaatagc aattgacata atacaaagtt ttgtattggc aggtattcaa cataccatga
    15481 atctgtacaa caacaaatag tttcttttag caaagttact tataataagt aggatgatat
    15541 gataaaagaa gaagtaagaa ttgacaaatg gttatgggct gtacgtttat tcaaaacccg
    15601 tacacttgct gctgaagcct gtaaaaaagg aaaaattacc attgatactg tatctgtcaa
    15661 accatcacgt actatccgtg taaacgatgt aatacaaata aaaaagcccc ctattacgta
    15721 tgcttttaag gtgttagcgg taacagaaaa cagaatgggt gcaaagttag tgccctcctt
    15781 tatggaaaat gtaacatccg aagaacaata tgctatttta gaattatcaa agatttcagg
    15841 ctttatcgat agagacaaag gaactggacg accaacaaaa aaagacagac gttcgttaga
    15901 atctttctta gataccgatt ggacagaaga gtaaaaaaaa gaaaacgtat gattatcgcc
    15961 cgtcaaaaaa gaaaagaaaa tattgctgaa tacattttgt atatgtggca tattgaagat
    16021 attatcagag catacaagtt agatattgaa gcaatacaca cacatatcat aatgcatttt
    16081 agccttgatg aagccacaag aaaagaaatg aaagaatggt acgaaagtct aatagaaatg
    16141 atgaataagg aacaagtgat gcatacagga cacttgcaaa tcaaccagag ttccgttcgt
    16201 ttgctttgtg attttcatgt agaattgctc gaaaatgaag acgaaaaaca gtatcattca
    16261 ttatatcatc aaacgctacc attaatcgaa gaattcagac ataaaaatgc actaagcgat
    16321 gtcccttcaa tcgaagtctg cttgcaattt atgtatggca tttggttgtt aaaaattcag
    16381 aaaaaagaga tttctaccga aaccaatcaa gcagtagtac aaattggtaa acttttagca
    16441 tttttgtcta agaaatttaa aaacaatgag gagaattaac aaaatacttg taactttggt
    16501 gcggtaaatt cgtttacact aataaataaa aaaaatcata tgaaaggaat tgtacttgct
    16561 ggaggctctg gaacacgttt gtatccaatt acaaaaagta tttcaaaaca aattattccg
    16621 gtttatgaca aaccgatgat atattatcca atctctgttt taatgcttgc aggcattaga
    16681 gaaattctaa ttatttctac acctcaagat atccatttat acgaaaactt attaggtgat
    16741 ggtactagtt taggtgtaaa atttacctat gctattcagc cctcaccaga tgggctagca
    16801 caagcgttta ttattgggga cgaatttatt ggcaacgact cagtatgtat ggtattggga
    16861 gataacatat tttatggata taatttttca aaacaactaa aagaagctgc aacactcgaa
    16921 gatggagctg tagtattcgg ttattatgta aatgaccccg aacgctatgg agtagctgag
    16981 tttgacacca atggcaaagt tttaagttta gaagaaaaac cagcgatacc aaaatctaat
    17041 tatgccgtta ctggcttgta tttttatagc aacgacgtga ttcaaaaagc aaaaggatta
    17101 aaaccatcgg cacgtggcga actggaaatt accgacttaa acaaacttta cttgcaagaa
    17161 gaacgcctaa aagtacaact acttggacgg ggcatggcat ggcttgatac aggcacacac
    17221 gacagcttgt tagaagcttc taattttatt gcaactattg aaaacagaca aggcttaaag
    17281 gttgcttgtt tggaagaaat tgcattcaat aatggcttta tcaacaaaga tcagttatta
    17341 aaattggcag agcctcttaa aaagaactat tacggtcagt atttattgaa aattgcatct
    17401 caaaaataac atcctttttt actagcaaaa acgtacacat ggaaattatt gaaacgaaaa
    17461 tcccaggttt atatattgtt caacccaaag tatttggcga tcatagaggt tatttttgcg
    17521 aaacatataa cgaaaaaaca tttcactctt tgggcatcac aaacaaattt tgtcaagaca
    17581 atttgtctaa atcatcgtac ggagttattc gcggtttaca ttttcagcta cacccacaca
    17641 gtcaagcaaa actagtttcg gcagtagtgg gcacggtata tgatgtagct gtagatatgc
    17701 gtgttggttc gcctacatat ggcgagtggg taggtatcga attaagcgaa gaaaataaaa
    17761 cacaattcct tatcccacaa ggatttgcac atggattttc agttcttagc gaaacagcta
    17821 ttttctctta caaatgtgac aatctatata caccatcagc agaaggaggt attttatata
    17881 acgatccaac cttacaaata gactggaaag ttccttttga aaaacaactt gtttcagaca
    17941 aagacacaaa acaccctacc ttgaaggatg tgaaaactaa ttttgtattt taattataaa
    18001 attgaatcgt atgacaaacg tattaataac cggagcttat ggtcagctag gaagcgaact
    18061 taaaaaactt gcaccccaat atacacagtt tacctatttt tttacggatg ttgatacgct
    18121 tgatatatgt aataaacaag caattttaca gttttgcata gataattcta ttcatttcat
    18181 tttaaattgt gccgcataca ctgctgtaga taaagcggaa gaaaaagagc aacactcttt
    18241 gtgttatgct atcaatgctg aagctgtaaa aaacttagga gaagtggctt cgcaaatagg
    18301 agccaatata atccatattt ctactgatta tgtatttgat ggcaaagcat atctcccata
    18361 cactgaagat ataacgacta atcctcaatc attgtatgga aaatcaaaac tattaggaga
    18421 acaatggcta atgaaatcat gtgctcatgc acttattatt cgtacctcat ggctatattc
    18481 tacttttgga aacaattttg taaaaacaat gataaagtta ggtgcagaac gtacggaact
    18541 caaggtgatt tttgatcaaa ttggcacccc tacctatgct gctgatttag caaatacaat
    18601 gttaaccata gtagccaaat caacagaaaa agctaccaat tacgtgcctg gcatatataa
    18661 tttctctaac gaaggcgtat gtagttggta cgacttcaca attgctatac atgctttata
    18721 tggcatttct acctgcaatg tgttacctat tgaaagtaaa gaatatccaa ccaaagctcc
    18781 ccgtccgcat tatagtgtcc ttaacaaaca gaaaataaag accacttttg acattactat
    18841 tcctcattgg caaacatcat tagccaattg tataggtaaa ttaaagacgt aataatcgcg
    18901 atgccttgct caaagtaagc acgttttacc ttggtaaata aaataaaaaa gaatgttaaa
    18961 taatttgtat agttcttttt ttacagtaac tttgggcgtt ctttttacaa ttaacgagaa
    19021 acactaacct ttattctaca aacaaatact atgtcagtta aaactgcaag agcttatatt
    19081 acacaggtaa ttggacctat tgtggatatc tattttgaag aaggtggata tgcccttcct
    19141 gaaatccatg atgcattaga aataatcaaa ccttcagcta aaaacattat tgttgagtgc
    19201 caccaacaca ttggtgagcg ttctgtgcgt accatatcaa tggattcgac agatggtcta
    19261 gtaagaggca tggaagttat ccctaaaaac aagccgatct gtatgcctat tggcaatcaa
    19321 ataaaaggac gattacttaa cgtaattggc gatgccatag atggaatagg cgaacttgat
    19381 agaaaaggag aatcccctat ccacagagac cctcccaaat acgaagattt aacgacaaca
    19441 aaagaagtac tatatacagg tattaaagtt gtggatttac ttgctcccta ctcacgtggg
    19501 ggtaagattg gactctttgg aggtgctggt gtaggtaaaa cagtacttat tatggaatta
    19561 atccataata ttgcaaaagg atacaatggt tattctgttt ttactggcgt aggcgaacgt
    19621 acacgcgaag gaaatgattt attgcgagaa atgattgaat ctggcgtaat caattatgga
    19681 gaagaattca aaaaaagcat ggaagaaggc gattgggatt tatccaaaat tgaccatgaa
    19741 gccttaaaaa cctcaatggc tacgctcgta tttggacaaa tgaatgaacc tcctggagca
    19801 agagcctctg tggcactttc tggattaacg gtagctgaat catttcgtga tgccgatgca
    19861 agcaatgctc aaggaaaaga tattttattt tttatagaca atatcttccg ttttacacaa
    19921 gcaggttcag aggtttctgc actactaggt agaatgccgt ctgctgtagg atatcaacct
    19981 accttagctt cagaaatggg attgctacaa gagcgtatta cttcgactaa gacaggtgca
    20041 atcacttctg ttcaagccat ttacgttcct gcagatgact taactgaccc agccccagca
    20101 accacattct ctcacttaga tgcaacaact gttttaagta gaaaaattgc agagctagga
    20161 atttaccccg cagtagaccc tcttgattca acatcacgta ttttggaacc aaatgttgta
    20221 ggcgaattac attacaaaac tgcattaagg ctagttgagc tattacaacg caacaaagag
    20281 ttacaagaca ttattgctat tttaggaatg gaagaattat ctgatgaaga taaattaatt
    20341 gtacatcgtt caagaagagt acaacgattt ttatctcaac catttcacgt tgctgaacaa
    20401 tttacaggga tacccggtgt ttttgtacca atagaagaaa ctattcgtgg gtttaatatg
    20461 attatggatg gagaagttga ccaataccct gagtctgctt tcaacttagt aggcaccata
    20521 gaggatgcaa tagaaaaagg gaaagctatt ttgaggggag aaaactaaat acatatgata
    20581 gtagaaattt atgcaccaca taaaacactt ttttctggca atgcattagc aatatcttta
    20641 cctggaaaaa tgggtaagtt tgcgatactt gataaccatg ctccactggt ttctgtacta
    20701 ggaaaaggaa atatctatct cacaaaaaag aatgaagaaa atatttcttt tagtattgat
    20761 agcggttttg tagaggtaaa taataatata gtaaaagtct gtgtagaaat taataaaaaa
    20821 taaatatagc atgagaaaat attaccaaat cattgttacg agtgtaattg tatctcaact
    20881 aataatttgg ttggtattta cctttttttt acctgaatat tgggatacca atttttttct
    20941 tatccctatt tattttttag caattgagat agtgttttgt aaattattgg aacggtttta
    21001 tgtagccaaa caaaaattaa attggtatgt tatttttaag ttaggaaagt tattattatc
    21061 aattattctt gcagctcttt atatacttgc aattcaaaca caagcaatcg catttttatt
    21121 aaaatttggt gtggtatacc tagtgttttt atttgttgag acttggatat tcttagatta
    21181 ccaaaaagca caaaaaggaa aatgaatcag tatttcaaat acatattgct atgttttact
    21241 ctcttgtttg ttgttagcta cacgcaagca gcgaataacc ataaagaaga tgagaaaccc
    21301 tttgatgcca atacatttat ttttggacat atagcagatg cttacgactt tcatattacg
    21361 tcagtaggac ataagcatat ttctgtacct ttattagtaa ttgtaaaagg gaaagaaaat
    21421 gggtggtctg tttttccgtc ctacagaata acacatggag atacctacaa gggttattat
    21481 atccaaaaaa aaggaagtta taaagggaaa attgtagaga cactatccaa cggagaaata
    21541 catagacctt ttgatttatc gataaccaag aatgtatttt ctattttact ttctatcttt
    21601 atattaattg ctctatttgt tcctgcagcc aatgcatata aaaagaatcc tttgggtgtt
    21661 cctactaaaa aaaatcattt attagaaatg gtcattctat caatccaaaa tgacgtagtg
    21721 aaaccctcag taggaaagga ttacgcaaaa tactcaccct acttgcttac agtttttttc
    21781 tttattctta taaccaattt aatgggcctt attccatttt ttccttttgg ggcgaatgta
    21841 actggcaata ttgccgttac catggtattg gctattttca cctatcttat tactaatatt
    21901 tttggatctc gcgaatattg gaaagaaatt ttttggccag atgtgcctgt tatgctgaaa
    21961 ttccctttac ctttaatgcc tttaatagag ttaatatcta catttacaaa gccatttgct
    22021 ttacttgtgc gtttgtttgc taacatattt gcaggtcata tgattatttt ggtattaatg
    22081 gctttaattt ttattttcgg tagcatcaat ccaggcgttg gatatggagt ttctattatt
    22141 tcaatcggat tctccatatt tatgtttatg ctcgaattac tggtggcttt tatacaagcg
    22201 tatgtattta ctactttgtc agctattttt ataggtttat ctcgagtaga acctcatcat
    22261 catcctaaaa aagaaataaa ttaaaataaa tcattcactt aaaacaaaaa aaactatgtt
    22321 attatcaatt attcttcaag cagtagcatc agcctctttg gcaaaattag gagcaagttt
    22381 aggtgcagca ttagctgtag ttggagctgg tttaggtatc ggtaaaattg gtagctcggc
    22441 aatggaagcc attgctcgtc aaccagaagc tgctagtgat attcgttcaa gtatgattgt
    22501 gtcagcagct cttattgaag gggctacttt atttgcaatt atcgtatgtg ctttggctat
    22561 tcttatttaa gtaaatcgcc actataaata cctttaaatt taataaatag tcttatggat
    22621 ttatttcttc cggaatcagg attggtaatt tggatgacca tagcctttgg tatcgtcttt
    22681 tttattttgt caaaatttgg gttccctatt attttgaaat ctattaatga acgagaaaag
    22741 tacattatca aatcgcttga tgcggctaat gaggctcaac aaaaactcct agaaatagaa
    22801 gacaaaggga aagaaattat tgccaatgct gaacaacaac aactcttgat agttaaagaa
    22861 tctcaagagt taaaaaagaa attagttgaa gaggctaaaa tgcaagcaca aaaggaagct
    22921 gataacatta tagaaaatgc tcgcattcaa attgaaaaag aaaaagaaac agctttatct
    22981 gatatacgta atgaagtact tcaggtatcc ttttctattg cagaaaaggt acttgaaaaa
    23041 gaactcgaaa ataaaaaagc tcatacccag tatgtagaaa agttattaga taatatgtca
    23101 atgtctcaat catagaatta tacatgaacg aaggtaaaat atcagttaga tatgctagag
    23161 caatccttaa tttagctaaa aataaaggta ttgaagatgc tgtgtacaaa gaaatgtttg
    23221 tactttcgga caacttaact cattacgcaa aagattttaa aaccgctttt gcccatcctg
    23281 acataaccga agaaaacaaa aaaaaattgt ttaaacttgc tgttggtaat accatctcat
    23341 catttacatc acaatctatt gattttattg tggaaaaaaa aagaagctca tttatgctac
    23401 gcattgcatt aatgtatcaa actttatatc aaatagaaca caatattatc attagcaatg
    23461 taataagtgc taaagaatta gacaaaaaaa cattagataa aataaaagcg tttgcaagta
    23521 aaacgtttga tgcatctgta gaactagtca gtacaataga tcccgactta ataggagggt
    23581 ttattttaga tattgccaac caacggctag atgcgagtat taaaggaaaa ttaaacaata
    23641 ttagaaaaat agtatactaa aaatatatcc aattatggca agcataaaag caagtgaagt
    23701 gtcgaatctg ctacaaatgg aactagaagg ttttagtgat aaactaaaat ttgaagagac
    23761 aggcaaggtg ttacaaataa gtgacggtgt agcaagggtt tttggattaa ctaaggtgca
    23821 atcgaatgag cttgtacttt ttgaaaatgg cacaaaagga gtagttttaa atctggaaga
    23881 agacaatgta ggtattgttt tattgggatc tacccgtgga attaaggaag gagacactgt
    23941 acaaagaaca ggaaatattg cttctatctt agctggcgaa ggactattag ggcgtgtagt
    24001 aaatacattg ggcgaaccta tagatggaaa aggcgaaatc aaaggcgaat tatttgaaat
    24061 gccgttggaa aggaaagctc caggtgtaat atttagacaa cccgtaaacg aaccactaca
    24121 aacaggaatt aaagccattg atgctatgat tccgattgga cgtgggcaga gagagttaat
    24181 cattggagac agacaaacgg gtaaaacaga aatagccatt gatacgataa tcaatcaacg
    24241 tagtaacttt ttagcaggag atcctattta ctgtatttat gtgggtatag gacaaaaagg
    24301 gtcgaccata gctggcattg taaaaacctt cgaagagtat ggtgccatgg attataccat
    24361 agtggtatct gcaacagcct ctgaccctgc tgcaatgcaa ttttatgctc cctatgcagg
    24421 agttgccatt gctgaatatt ttagagatac aggtcgtcat gcattaatta tttatgatga
    24481 tttatcaaaa caagcagttt cttatcgtga agtatcgttg gtacttcgtc gtccacctgg
    24541 tcgtgaagca tatcctggtg atatctttta tttacattca cgtttgttag aaagagcagc
    24601 taaaatcatt aaaaatgatg ttgtggcacg taatatgaat gatttgccag attccatcaa
    24661 acacttagta aaaggaggag gctctattac agccttacca attattgaaa cacgagaagg
    24721 agatgtttct gcatttatcc ctaccaatgt catctccatt actgacggac agatattctt
    24781 agaaacaagt ctgtttaatg caggtattcg tcctgcaatt aacgtaggta tatctgtatc
    24841 acgcgtaggc ggaaatgccc aaataaaagc aatgaaaaag attgcaggga cactcaaact
    24901 tgaccaagct cagtaccgcg aactagaagc tttttcaaaa tttggagcag atcttgatgc
    24961 tggtaccaag ttggtgctaa ataaaggaag taaaaatgtt gaaatattaa aacaaaaact
    25021 tcatcaacca atgcctgtgg aaaagcaaat tgcaattata ctttgtgggg tacgcgggta
    25081 tttgtctcat gtaccactaa ataaggtaca agaatttgaa aataacttgt tagaaattct
    25141 tgaagctaaa tataaaaaag atgtattaga catactacaa aagggcgatt tgacagaaga
    25201 tgttttaaca aaaattaaag atgtcgcttt tacactttgt aaacaatatg aataatctaa
    25261 aaattgtgag taaaaaataa tggcttcttt aaaagatata cgtgcacgga ttctttctgt
    25321 tagatcaacg aaaaaaatta ccagtgcaat gaaaatggtt tcttcatcga aattgcataa
    25381 aacagagaca ttgtgcctta attttttacc atacaaaaat gctttgtgtg aaagtttagc
    25441 taactatttg aactcattag acgaaaaaat atctgttcaa gtagctgaac aacgcccaat
    25501 taagcgtatg gcaattatat gtttttcttc caattcaggt ttatgtggta cgtttaacac
    25561 aaatattgca aaattagcat ctgaaaaaat tgatcaatac gcttatttag gaaaagaaaa
    25621 tatttatgtt tatactattg gaaaaaaaat aactgactac ttgcaaaaac tcaagtaccc
    25681 tatccatgcc cactacaata atttattaga taaccctagt tatgatgaag ttgtcaaatt
    25741 ggccgacatg ttgactgaac tatttatttc aaaacaaata gactgtgtag agttattgta
    25801 taatcactac aaaaacactg cttgccaaac acctacatct gaaatactcc ttccactatc
    25861 atctatgcaa cttacaagaa ctacgaaatc tcaaccgctg tatattgtgg aaccctctaa
    25921 agaagaattt attaatgccc tagcaccgaa attaattcga atcaaattat atgctgcttt
    25981 tttagattca tttacagccg agcatgctgc tagaactact gcaatgcaaa ttgctaccga
    26041 aaatgccgaa gaaatgatta agacaataac tcaacaatac aataaaaaac gacaagaagt
    26101 tattaccaat gagttgttag atattgtagg aggatctgag gctttacgtc aagattaaca
    26161 ctaaactgaa aacaatggaa ataactagag tttttgatat tttagaaaat tataaaatta
    26221 taaaaccaaa ccaacctata gcactagcac ataaaaaaga aggcaactgg ataaaataca
    26281 gtattgacga atacatctac caaacaaatt taatatcagc aggattgcta tacaatgggg
    26341 taaaaaaagg agacaccatt gctataataa gctctaatag agctgaatac aatattgtag
    26401 atatggctgc aatgcaaatt ggagcaatac ctgtccctat tt
//
LOCUS       DAOSRP010000010        22211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2525_length_22211_cov_5.564272, whole genome shotgun sequence.
ACCESSION   DAOSRP010000010 DAOSRP010000000
VERSION     DAOSRP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22211
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_22211_cov_5.564272"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..922
                     /gene="trpA"
                     /locus_tag="PLJ82_01370"
     CDS             146..922
                     /gene="trpA"
                     /locus_tag="PLJ82_01370"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOR40193.1"
                     /translation="MDRIASLFATKKNRIISVYFTAGFPQLHDTVTIIKELAHQGVDM
                     IEIGIPYSDPMADGPVIQHSSQQALENGMSQKLLFEQLATIRQEVSIPLVFMGYLNTI
                     MQFGFERFCQQAANIGIDGLIIPDIPLDVYVKEYKTIVEQYGLKFILLITPETTEERI
                     RQIDAVSSGFVYMVSSAATTGSQQSFDTEKQAYFKRIDAMKLKNPTVVGFGVSNNATF
                     EAACAYANGAIIGSEFIKQLQNQPSIPLAVSTLLSTIKGS"
     gene            complement(923..1861)
                     /locus_tag="PLJ82_01375"
     CDS             complement(923..1861)
                     /locus_tag="PLJ82_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018032.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOR40194.1"
                     /translation="MNIPTHFSPTKRYLDYSQYMLAFFPERIQKISIHAGFTCPNRDG
                     SKGYGGCTFCNNESFSPAYCSSHTSISQQIEEGISFFGKKHAHQKYLAYFQSYTNSYG
                     SMEVIKQKYSEAIHHPAISGIVIGTRPDCMSDELLDYLHEIQKNTFVLVEYGVESTNN
                     NTLELINRGHTYEESVDAIMRTHAKGIPVAAHIILGLPSENRETILNHATQLATLPLA
                     IIKLHQLQIIKNTPLAKQYTDNPSFIPLYSLQEYIDLCIDFMEHIPSHICLERFVSQT
                     PTEYRIAPNWGIKNHEFIAKLDKRLQERNTFQGRLC"
     gene            2052..3641
                     /locus_tag="PLJ82_01380"
     CDS             2052..3641
                     /locus_tag="PLJ82_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOR40195.1"
                     /translation="MYSEDDLIWLSENGITPESLEKQLQIFTKGVEPPAIKRIATCND
                     GIRVVNDAEVEMYQTAWNDYIENNPDKTTHFIPASGTANRLFRALYRFYYSEHTEPQT
                     NIEKNFFKHLQSFAFFNELNVICIQKEKKDIHELIEQKQYKLILEYMFFEKGANYSQL
                     PNALFKFHTDKSMRYAAFQKYLPKKIAKYASFEHTRTPIQENLLECAMVSGVKKGNVR
                     VCFTVNKEHLNQIAEYIEEYKKPIEKKLSVSLDVHLSVQHPSTQTVIVKDDNSFFRDK
                     DNKITFTYAGHGALLENLNAIDAEIVFIKNIDNAVPDTLKKTTSSYKKMLAGILLSTR
                     NKIRYYVDLLDMPNLPEDKLIEIIHFVENELNVKKQSILSLPKGVLLQYLKEKLDRPL
                     RVCGMVKKEEEQGGEPFWVEQKDGTMSLQIVDFYQISNDQKALELFDTATHFNPVDIV
                     CSIVDKDGSKYDLSKFVDEEAYIIARKKKKDEWLKRLEFPGLWNGAMAHWNTIFVEVP
                     IKTFNPVKTINDLLRYEHQNQ"
     gene            3807..5816
                     /locus_tag="PLJ82_01385"
     CDS             3807..5816
                     /locus_tag="PLJ82_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOR40196.1"
                     /translation="MEELKLLQNDPWLAPYKEAIHGRYAYALHKEQALVQEMNSLADF
                     ATGYLYFGLHKISDGWVMREWAPNATEVFLVGDCNNWQTSEEYRLHRKENGVWEGIFK
                     EDALKHQQLYKLFVCWNGGCGERIPAWVRRVVQDENTKIFSAQVWNPEQKYTFKVNQF
                     VPNKDPLVIYECHIGMATEEEKVGSYLEFRDLVLPRIKKAGYNCIQIMAIQEHPYYGS
                     FGYHVSNYFAASSRFGTPEELKSLIDTAHEMGIAVIMDIVHSHAVKNETEGLGRFDGT
                     PYQYFHDGPRREHPAWDSLCFNYGKHEVLHFLLSNCKFWMDEYKFDGFRFDGVTSMLY
                     LSHGLGESFSGYADYYNGNQDGDAICYLTLANKLIHQVNPNAISIAEEMSGLPGLANK
                     FEDGGIGFDYRMAMGIPDLWIKYIKEYTDENWKPGHLFWELTNRRYDEKTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMEKGHSTYMVDRGVALHKMIRLLTASTINGGYLNFMG
                     NEFGHPEWIDFPRQGNGWSYKYARRQWSLVDNPNYLYHSLAMFDRGMIDILTKTDKFQ
                     DSGVYKCWDHSGDQVLAFARGGELLFVFNFHPYNSFTDYGMLVDKGEYEVVLNTDAKE
                     YGGFGLCDDSIRHFTIPTNVKGEEKEWIKLYLPSRTAMVLRKVSK"
     gene            5818..6543
                     /locus_tag="PLJ82_01390"
     CDS             5818..6543
                     /locus_tag="PLJ82_01390"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOR40197.1"
                     /translation="MFYQDSIHKKSVNQKQLALLVDPDKYSQESLALFVELAKETLPD
                     CIMVGGSLVAGSVEQVVQYIKLHTNIPVVLFPGNSHQLCNEADALLLLSLLSGRNADF
                     LIGQHVVAAKAIRDSGIESISTGYILIDGGCSTSVEYMSNTKPIPCDKVEIIVATAIA
                     GELIGNRMVYLEAGSGAINPVSKEAIQAVRKAISLPIIVGGGIRTVEQVKDAYLAGAD
                     MIVVGNALEKDRFFMRDLAQIRG"
     gene            complement(6607..8442)
                     /locus_tag="PLJ82_01395"
     CDS             complement(6607..8442)
                     /locus_tag="PLJ82_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR40198.1"
                     /translation="MKRFIILLKRFIPPYKKYVGLNVLFNLLSTIFSLFSFALIIPIL
                     EILFNTKAVVYELQSTHNVPMSELFDVLKNNLYYYTTDLIDKSGALTVLIILSSFLIL
                     MTFLKVATAYLGSFFMVSIRTGVLRDLRNMIFAKIVHLPIGFFNEERKGDIMSRMTLD
                     VNEVEASIMSSLDMLFKNPLMILTYIIVMFTISWELTLFVMVLLPISGYFIGQVGKSL
                     KRKSLEGSILTGELTSQIEETLGGLRIIKAFNAEKKIISRFVALNNRLRGTLNKIQGR
                     YLLAHPMSEFMGTVVIATVLCYGGNLILTSNSEALTASVFIYYILIFYSIINPVKELS
                     KAAYSVQKGMASLERIDKILHTQNSLHTVNGKEISGFNHSIEFKNVSFKYQNAWVLRN
                     INLTIEKGKTIALVGQSGSGKSTLVDLIPRFWDVVEGEICIDGVNIKEINLHSLRDLM
                     GNVNQEAILFNDTFYNNIAFGVENATMDEVIAAAKIANAHDFISATEQGYDSNIGDRG
                     SKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKLVQEAIEKLMKNRTSVVIA
                     HRLSTIKNADEICVLLDGKIVERGTHEALLQENGVYKKLQETQTV"
     gene            complement(8460..9806)
                     /gene="purB"
                     /locus_tag="PLJ82_01400"
     CDS             complement(8460..9806)
                     /gene="purB"
                     /locus_tag="PLJ82_01400"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOR40199.1"
                     /translation="MTNLNLLTAISPVDGRYRSKVDELTNYFSEYALIRYRVQVEIEY
                     FIALCELPLPQLNNIEKKVFPVLRSIYTSFSVADAQKVKDTESITNHDVKAVEYFIKD
                     KFDEIGLSDYKEFIHFGLTSQDINNTAVPLSIKDCLYTVYLPVLQQLVDELEQLATKW
                     EHIPMLAKTHGQPASPTRLGKEIRVFVSRLEQQIVLLKQTPISAKMGGATGNFNAHHV
                     AYPTINWIDFGNSFVKNYLGLEREPYTTQISNYDNLAALFDAIKRINTILIDMNRDFW
                     TYISMEYFKQKIKVGEVGSSAMPHKVNPIDFENAEGNLGIANAILEHLAGKLPISRLQ
                     RDLTDSTVLRNVGVPMAHNLIAIKSTLKGLSKLILNEASLKRDLDNCWNVVAEGIQTI
                     LRRENYPKPYETLKELTRTNTEVNAESIASFIETLQVSESVKAELRAITPHNYTGL"
     gene            complement(9954..11282)
                     /locus_tag="PLJ82_01405"
     CDS             complement(9954..11282)
                     /locus_tag="PLJ82_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOR40200.1"
                     /translation="MEHVSDVAQHVAMPIWASAPFALMLIMIAIGPLLWEHWWEQNKN
                     KLLVSFLLSVPTVIFLIINGFSHDLEHQIMFDYVPFIILLTSLYVISGGLFLTGDIQA
                     KPKINTLFMAIGYVLASIMGTTGAAMLLIRPLITTNSQRIYKTHTILFFIAAVANAGG
                     LLTPLGDPPLFLLYLRGADFGWFLHMLPEWLFIGTVLIGLYYLLDRRYYKKEKPEAIE
                     SDNANVEPLRIGGSKNFFLLIGVILCVAFVNVNYIPEMGNPHGSLWIKYLREIILIGF
                     ILVSMDITKKKTRELKGFSWTPIYEVAALFLGIFITMVPALLYLKENAASFGLSESWQ
                     FYFMTGSLSSFLDNAPTAVAMHGVALGLVQEGAAVAEVAGVPELLLTAISMGSVLFGS
                     MTYIGNGPNFMVKAIAESSGIKMPSFFGYIFKFSLVILLPLYILTYFLFV"
     gene            complement(11331..13118)
                     /gene="lepA"
                     /locus_tag="PLJ82_01410"
     CDS             complement(11331..13118)
                     /gene="lepA"
                     /locus_tag="PLJ82_01410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOR40201.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEETSTVTGKEMQAQVLDNM
                     DLERERGITIKSHAIQMNYIYEGEKYVLNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYMAIEHDLEIIPIMNKMDMDSAMPEEVEDQIVELLGCKSDEIIKA
                     SGKTGLGIQEILQAIVKRIHPPKGDPEAPLQCLVFDSVFNSFRGIITYMKIENGTIRK
                     GDLVKFFNTGKEYFADEIGVLRLGMEPREELRCGDVGYIISGIKVSKEVKVGDTITHV
                     KRPCEKGIEGFEEVKPMVFAGIYPIDSEDFEDLRASMEKLQLNDASLTFEAESSAALG
                     FGFRCGFLGMLHMEIVQERLDREFDMDVITTVPNVQYKIYTRKNEVLDVHNPSGMPDI
                     TLIDRIEEPYIRASIITRTDFIGSIMSLCLGKRGELIKQDYISSNRIELIYDMPMGEI
                     VFDFYDKLKSISKGYASFDYHISGFRPSKLVKLDILLNGESVDALSSLTHFDNAVTIG
                     RRMCEKLRELIPRQQFDIAIQAAIGSKIIARETIKAVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFMEVLKLD"
     gene            13342..14655
                     /gene="ffh"
                     /locus_tag="PLJ82_01415"
     CDS             13342..14655
                     /gene="ffh"
                     /locus_tag="PLJ82_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOR40202.1"
                     /translation="MFENLSERLERSFKILKGQGKITEINVAETLKDVRKALLDADVN
                     YKIAKTFTDTVKEKALGMNVLTAIKPNELMVKIVRDELALLMGGTSVDINTKGSPAVI
                     LMSGLQGSGKTTFSGKLAKMLKSKKGKNPLLAACDVYRPAAIEQLKVLGEQIGVPVYS
                     EIENKNPIQIAQNAIAFAKKNGHDLLIIDTAGRLAIDEQMMNEIAAIKQAIQPQEILF
                     VVDSMTGQDAVNTAKEFNDRLDFTGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMEAIDQFHPERMADRILGMGDIVSLVEKAQEQYDEEEARRVAKKIAKNQFDFNDFMG
                     QIQQIKKMGNLKDLAAMIPGMGKAMKDVDIKDDAFKGIEAMIQSMTPAERENPQLLNG
                     TRKMRIANGSGTSIQEVNKLIKQFDGIRKMMRMVQTNGNKFPKFK"
     gene            14663..15544
                     /gene="folD"
                     /locus_tag="PLJ82_01420"
     CDS             14663..15544
                     /gene="folD"
                     /locus_tag="PLJ82_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOR40203.1"
                     /translation="MQLIDGKAIADIIKQEIAVEVATIKSMGQRPPHLAAILVGHDGG
                     SETYVAHKVKACEQCGFNSTLIRYEDTVTETELLAKISELNNDTAIDGFIVQLPLPKH
                     ISEQKIIEAIDYRKDVDGFHPINVGRTSIGLPAFVSATPAGIMELLKRYGIATAGKHC
                     VVLGRSNIVGKPVANLMIQKAYPGNATVTVCHSYSADLVEQCQKADIIIAALGQPNFV
                     KGNMIKDGAVVIDVGTTRVPSTTTKSGFKLTGDVLFDEVAPKCSYITPVPGGVGPMTI
                     VSLMRNTLLAAKKELFK"
     gene            15954..17300
                     /locus_tag="PLJ82_01425"
     CDS             15954..17300
                     /locus_tag="PLJ82_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOR40204.1"
                     /translation="MRQKKVAWKYRVPFLLLAIVAIVSLIPVDHETLIPLGGELLEVN
                     HADTSWLLMASALVLLMTPGLAFFYGGMVRYKNLVSTLLQSFIALGIISVLWIVVGFS
                     LAFGESVGGFIGNPTSFMFFKNVGLTPHPDFGPTIPFALFAMFQLKFAIITPALITGG
                     LAERVRFTSLMLFLILFSLLIYAPLAHWTWHPEGFLRKWGVLDFAGGTVVHISAGFAA
                     LSGAIFLGKRKGVNSEARPTNIPYILLGTGLLWFGWFGFNAGSSLAANDMAVRAFVNT
                     NISSATAMMTWLFVDGAMGKKRSAAGAAIGAVVGLVAITPAAGFVTVGQSIFFGFVAS
                     LVSYTAVQLKSKTKIDDTLDVFPCHGLGGIVGMLLTGVFAVENGVGVGLIHGNVTQFM
                     FYLLALLIVGVFTFGGSYLLYKVTNLILPMRVTAEQEEIGLDITQHGEEAFDLSTK"
     gene            complement(17286..17795)
                     /locus_tag="PLJ82_01430"
     CDS             complement(17286..17795)
                     /locus_tag="PLJ82_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40205.1"
                     /translation="MYSQIRVEKYLYPEYHLDSKVILEENFDEPLLIQKNKNYRLEEI
                     NGKRIYSVFKDSWYLNILPGRISKIPEQWEIKCNITINSTQFANQEKINPALYEEAFT
                     IYGESEDKLYFTFNASGHACVYYFNGSQYKIYKEVEFPKFNKNSEITIRRIDNDIYFY
                     IDYFYLFSR"
     gene            complement(18157..19017)
                     /locus_tag="PLJ82_01435"
     CDS             complement(18157..19017)
                     /locus_tag="PLJ82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOR40206.1"
                     /translation="MRQLKIVKSITNRESASLDKYLQEIGREDLITVEEEVELAQRIK
                     KGDRIALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIPVDKITDTLKVSGRHISVDAPFVEGEDNSLLDVLVNDDSPVADRFLLNESLATE
                     IDRALNTLSEREKNIIVSFFGISCQEMTLEEIGDKFGLTRERVRQIKEKAIRKLRSSS
                     KSKLLKGYLG"
     gene            19200..19560
                     /gene="rnpB"
                     /locus_tag="PLJ82_01440"
     ncRNA           19200..19560
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLJ82_01440"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            19579..20919
                     /locus_tag="PLJ82_01445"
     CDS             19579..20919
                     /locus_tag="PLJ82_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOR40207.1"
                     /translation="MARFTTLIIFFKSALHFLCFDIWRITDGEVTAAKNRIYTIIKVF
                     VISVRRFFEDKIFAKSAALTYYTLLSVVPVLALVIAIGKGFGVQYYLENQFVAIFPSH
                     STVLKEAFFLVDSYIQQIQGGVFFGIGFVLLFWSVLNMFSEIEIAFNDIWQIKKLRNT
                     FRRFADYFSMMLILPVLLIVSSGLSIYLNGTIQDTVLSSLIVPVWYGFLKILPFIINI
                     VLFTLMYLFFPNTKVRFIAALFAGVVAGVLFQLFQMLYISGQIWISYYNAIYGSFAAL
                     PLLLLWLWASWSIILYGAEFAFSVQNIKNYEFESDVKNISRRYENFLFVLISSVIVKR
                     FAEKLPAMNAEELSTHYNIPIRLVNRIVSKLLDAGIIVESISTVKKTEEIVYQPAIDI
                     QHLTLAYLFEQIDGLGSENFKIDITHEHAQPWQATLFVQNSTQSYASSVLLKDL"
     gene            complement(20926..21963)
                     /gene="mltG"
                     /locus_tag="PLJ82_01450"
     CDS             complement(20926..21963)
                     /gene="mltG"
                     /locus_tag="PLJ82_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOR40208.1"
                     /translation="MNYLRNHKTLLILVAVIGITASGLMLNAFILPNLNGNGNKKQYL
                     FVYPEQTWSDVINTLTEMEVVNSTFSLKLAIALKYADMPPKTGKYDIEPNLSNRYLLN
                     RIAHGLQSKIILRVVSTRNTQQVARNLSRQLMVDSTSLMKQFHNPTLLKKYELTPTNS
                     LCLFLPFNAEITWDTPIEEIIQLIEKEYTTFWNDNRKQLAKKINLTPSEIHILASIVE
                     EETNNFEDKKMVAGLYINRLRKDMLLQADPTVRYATGNFSLNRILKRHLAINSPFNTY
                     IYKGLPPAPIRIPTQESIDAVLNYTKHPYVYMVAKSDFSGTHNFSRTFDEHLRKARAY
                     QKQLNKRGIFE"
     gene            22082..22168
                     /locus_tag="PLJ82_01455"
     tRNA            22082..22168
                     /locus_tag="PLJ82_01455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22116..22118,aa:Ser,seq:gct)
BASE COUNT         6997 a         3618 c         4125 g         7471 t
ORIGIN      
        1 gatgtagaag aaattaaaaa gattgcacat ccgcaattgg tagcgataga tgtaaacagt
       61 cgttttgaaa catctccagc aaccaaaaat atcaaccaat tacaacaatt tatacaagaa
      121 ataaaacaac taaatacata ctaacatgga taggatagca tcattatttg caactaaaaa
      181 aaatcgaata atatcagtgt attttacagc aggatttcct caattacatg ataccgtaac
      241 tattataaag gagcttgcac accaaggcgt ggatatgata gaaataggga ttccttattc
      301 cgacccaatg gcagatggtc cagttataca acatagtagt cagcaggcac ttgaaaacgg
      361 catgtcgcaa aaattgcttt tcgaacagtt agcaactatc cgtcaagaag tgtctatccc
      421 gttggtattt atgggctatt taaatactat tatgcaattt ggatttgaac gcttttgtca
      481 gcaagcagca aacattggca ttgatggatt aattattcca gatatacctc ttgatgtgta
      541 tgtaaaagaa tacaaaacca tagtagagca atatggatta aaatttattt tattgattac
      601 acccgaaacc acagaagaac gtattcgaca aattgatgcc gtaagtagtg gatttgttta
      661 tatggtatct tcggcagcta ccacaggctc gcaacaatca tttgacacag aaaaacaagc
      721 ttatttcaaa cgcatagacg caatgaaact aaaaaatcca acagtagtag gctttggcgt
      781 atccaataat gctacgtttg aggctgcttg tgcgtatgcc aacggagcaa ttataggcag
      841 tgaatttata aagcaattac aaaatcaacc atctattccg ttagctgtga gtacattact
      901 ctcgacaatt aaagggtcgt aattaacaca aacgaccttg aaatgtgttg cgttcttgca
      961 aacgtttatc cagtttagca ataaactcgt ggttttttat gccccaattg ggtgctatac
     1021 gatattcggt aggcgtttgc gatacaaaac gttcaaggca aatatgcgaa ggtatatgtt
     1081 ccataaaatc gatacacaaa tcaatatatt cctgtaaact gtacaacgga ataaacgatg
     1141 gattatctgt gtattgtttt gctaaaggtg tattttttat aatttgaagt tgatgcaatt
     1201 tgatgatagc cagcggaagt gtggctaatt gtgtggcatg atttaatatc gtttctctgt
     1261 tttcagatgg caatcccaaa ataatatgtg ctgccacagg aatgcctttg gcgtgtgttc
     1321 gcatgatggc atcaacactt tcttcgtacg tatgtcctcg atttatcagt tcaagcgtgt
     1381 tgttattggt actttctaca ccatattcaa ctagtacaaa agtatttttt tgtatttcat
     1441 gcaggtaatc aagcaattcg tccgacatgc agtcgggtcg tgtgccaatt acaatacccg
     1501 aaatagctgg atggtgtatg gcttcgctgt atttttgttt aataacttcc atacttccgt
     1561 acgaatttgt atatgattga aagtaggcaa gatatttttg atgtgcatgc tttttgccaa
     1621 aaaaagaaat tccttcctct atttgttgtg aaatggatgt gtgagagcta caatatgctg
     1681 gactaaacga ttcattgttg caaaacgtac aacctccata tcctttgctt ccatctctat
     1741 tggggcatgt aaatcccgca tgaatagata ttttctggat tcgttctgga aaaaacgcaa
     1801 gcatatactg tgaataatca agatatcgtt ttgtgggcga aaaatgggtc ggaatattca
     1861 tgctttatta tttttatagt gcggcatagt tggcttaata caatatccat gcatgcaaaa
     1921 aagctacgtg caaaggtacg atacaaaatt gaagtatcga aatagtacga ataaaaatca
     1981 atacgtaata tttttttgaa gtatcactat taagttgtat ttttgttgtt cttaattaaa
     2041 agtaagatag gatgtatagt gaagatgatt taatttggct ttcagagaat ggtattacgc
     2101 ccgaatcgtt agaaaaacaa ttgcaaatat ttaccaaggg agttgaaccg ccagctatta
     2161 aacgtatagc aacatgcaac gatggtattc gtgttgtaaa tgatgctgag gtagaaatgt
     2221 atcaaacagc atggaatgat tatattgaaa ataacccaga taaaaccact cattttatac
     2281 cagcatcagg cacagctaat cgtcttttta gagcattgta ccgtttttat tattcagaac
     2341 atacagaacc tcaaacaaat attgaaaaga atttttttaa acatttgcaa agttttgcct
     2401 ttttcaatga gctaaatgtg atttgcattc aaaaagaaaa aaaagatata cacgagttaa
     2461 ttgaacaaaa gcagtataag ctcattctcg aatatatgtt ttttgaaaaa ggggcgaact
     2521 atagtcaact ccccaatgca ttgtttaagt ttcatacgga caaatcaatg cgatacgctg
     2581 cttttcaaaa atatttaccg aaaaaaattg ccaaatacgc atcattcgaa cacacacgca
     2641 cacctattca agaaaattta ttggagtgtg ctatggtgtc tggtgtaaaa aaggggaacg
     2701 ttagagtatg ttttaccgta aataaggaac acctcaatca aatagccgaa tatattgaag
     2761 aatataaaaa acctattgaa aaaaaattat cagttagttt agatgtacat ttatctgttc
     2821 agcatccgtc tactcaaaca gttattgtta aagatgataa ttcttttttt cgcgataaag
     2881 ataacaaaat aacctttacc tatgcaggtc atggagcttt attggagaat ttgaatgcaa
     2941 ttgatgctga aatcgtattt atcaaaaata ttgataatgc agttcccgac actcttaaaa
     3001 aaacaacatc aagttataaa aaaatgcttg ctggcatttt gttgtctact cgtaacaaaa
     3061 ttcgttatta tgtagatctt cttgatatgc caaatttgcc agaagataaa ttaattgaaa
     3121 ttattcattt tgttgaaaat gaactgaatg taaaaaaaca atctattttg agcttaccaa
     3181 agggcgtttt gttgcaatat cttaaagaaa aattagatag acccttacgt gtatgtggaa
     3241 tggtaaaaaa agaagaagaa caaggcggag aacctttttg ggttgaacaa aaagatggta
     3301 ccatgagctt acaaattgtt gatttttatc aaataagtaa tgatcaaaaa gccttggaat
     3361 tgtttgatac agctactcat tttaatcctg tagatattgt ttgttctatc gtagataagg
     3421 atgggtctaa gtatgattta tctaaatttg tggatgaaga agcatatatt attgctcgta
     3481 aaaagaaaaa agatgaatgg ttaaaacgcc ttgagttccc aggtttatgg aatggagcta
     3541 tggctcattg gaatacgatt tttgtagaag tgcctattaa aacatttaat cctgtaaaaa
     3601 caattaatga tttattacga tacgagcatc aaaaccagta ggcatggcaa catttgatta
     3661 cgtattaaaa tgaaatttgt aagtttttgt ttttttctta ttataaaagt acgataaaaa
     3721 tatagatttt ctttgctatt agtagaaaat actctatatt tgtcaagata tttttctaaa
     3781 ccacatacca cacgctcttt gtagatatgg aagaactaaa attattacaa aatgaccctt
     3841 ggttagcccc ttataaagag gcaattcatg gacgatatgc ctatgctttg cataaagaac
     3901 aagctcttgt tcaagaaatg aattctttag ccgattttgc aaccggttac ctttattttg
     3961 gattacacaa aatatcagac ggctgggtaa tgagagagtg ggctcccaat gcaactgaag
     4021 tttttcttgt tggagattgt aataattggc aaacttctga ggagtaccgt ttacatcgta
     4081 aagaaaatgg tgtatgggaa ggaatattta aggaagacgc tctcaaacat caacaattgt
     4141 ataaactttt tgtttgttgg aatggcggtt gtggagaaag aattcctgcc tgggtacgtc
     4201 gggtagttca agatgaaaac acaaaaatat ttagtgctca agtgtggaat cctgagcaaa
     4261 agtatacatt taaggtaaat caatttgtcc ccaataaaga tcctctggta atttatgaat
     4321 gtcatattgg tatggcaaca gaagaagaaa aagttggatc ataccttgag tttagagacc
     4381 tagtactgcc tcgtatcaaa aaagcgggat acaattgcat tcaaataatg gcaatacaag
     4441 agcacccata ttatggttct tttggatacc atgtgtcaaa ttattttgca gcatcctcac
     4501 gttttggtac cccagaagaa cttaaatcct taatagacac agcacacgag atgggtattg
     4561 ctgttattat ggacattgtt cattcgcatg ccgtaaaaaa tgaaacagaa ggattgggtc
     4621 gttttgatgg tactccttac cagtattttc acgatggtcc acgtcgcgaa catccagctt
     4681 gggactcatt gtgttttaat tatggcaaac atgaagtgtt acactttttg ttatctaatt
     4741 gtaaattttg gatggacgaa tataaattcg atggtttccg tttcgatggg gtaacctcta
     4801 tgttatattt aagtcatggt ttgggcgagt cttttagtgg atatgctgat tattataatg
     4861 gaaatcaaga tggagatgcc atttgttacc ttacgttggc aaataaacta atacaccaag
     4921 taaatccgaa tgcaatatcg attgccgaag aaatgagtgg tttacctggt ttagccaata
     4981 aatttgaaga tggtggcatt ggatttgatt atcgcatggc aatgggtatt cctgatttat
     5041 ggataaaata cataaaagaa tatactgacg aaaattggaa accaggtcat ttattttggg
     5101 aattaaccaa cagaagatac gacgaaaaaa caattagtta tgctgaaagt catgatcaag
     5161 ctttagtggg tgataaaaca attattttcc gtttgattga tgccgatatg tattggcaca
     5221 tggaaaaagg acattctacc tatatggtag acagaggtgt ggctttacac aaaatgattc
     5281 gattacttac agcatcaact atcaatggtg gatatttaaa ctttatgggc aatgagtttg
     5341 gtcatccaga atggattgat tttccacgtc aaggtaatgg ttggtcgtat aaatatgctc
     5401 gtagacaatg gagcttggta gataacccta attatttata tcactctttg gcaatgtttg
     5461 atagaggtat gattgatata ttaaccaaaa cagataaatt tcaagattca ggtgtttata
     5521 aatgttggga tcattctggc gatcaggtgc ttgcgtttgc acgtggtggt gagttattgt
     5581 ttgtatttaa ttttcatccg tataattcgt ttacagacta tggcatgttg gtagataaag
     5641 gggaatacga ggttgtattg aatacagatg caaaagaata tggtgggttt ggattatgtg
     5701 atgatagtat tcgacatttt accataccaa ctaatgtgaa aggggaagaa aaagagtgga
     5761 ttaaactcta tctcccatct cgtacagcca tggttttacg caaagtctca aaataaagtg
     5821 ttttatcaag atagtataca taaaaaatca gtcaaccaaa agcagttagc actacttgtc
     5881 gatcctgata aatattctca agaatcactt gcattatttg ttgagttagc taaagaaaca
     5941 ttacctgatt gtattatggt gggtggcagt ttggtggctg gttctgtaga gcaagtagtg
     6001 cagtatatca aattacatac aaacattcct gtggtgctat ttcctggaaa tagtcatcaa
     6061 ttgtgcaacg aagccgatgc tttattgttg ttgtcgttgt tatctggtcg caatgctgat
     6121 tttttaattg gtcagcatgt agtagcggca aaagccatac gcgattcggg tatcgaatct
     6181 atttctacgg ggtatatcct tattgatgga ggatgttcta ccagtgtgga atatatgagt
     6241 aacactaaac caattccatg cgataaagta gaaattattg tagccacagc catagctggc
     6301 gaactaattg gcaatcgtat ggtgtattta gaagctggaa gtggagccat caatcctgta
     6361 tcaaaagaag ccattcaagc tgttagaaaa gcaatttcat taccgattat tgttggtgga
     6421 ggaattcgca cagtagagca ggtaaaagat gcgtatcttg ctggagctga tatgattgta
     6481 gtaggcaatg ccctagaaaa agataggttt tttatgcgtg atttggcaca aattagaggc
     6541 tgaactaacg atttatttgc gttctcatta cgatattgac tgttaggttt tgaccgattt
     6601 tgtgtattac actgtctgcg tttcttgcag ttttttgtac acaccatttt cttgcaacaa
     6661 agcttcgtgt gtgcctcttt ctactatttt accatcaaga agaacacaaa tttcgtcagc
     6721 atttttgata gtagatagtc ggtgagctat aacaacactg gttctatttt tcatcagttt
     6781 ttcgatggct tcttgcacta atttttctga ttcagtgtct aaagctgagg tagcttcgtc
     6841 taaaattaag ataggagggt tttttagtat tgctctagca atgcttatac gttgacgttg
     6901 tccaccagat aacttactgc ctctatctcc aatgtttgaa tcataccctt gttcggtagc
     6961 tgaaataaaa tcgtgtgcat tggcaatttt tgctgcggca attacctcat ccatcgtggc
     7021 attttctaca ccaaatgcaa tattattata gaaagtatca ttaaataaaa tggcttcttg
     7081 attcacattc cccatcaaat cgcgtaagct atgcaagtta atttctttaa tatttacacc
     7141 atcaatacat atttctccct ctaccacatc ccaaaaacga ggtatcaaat cgactaaggt
     7201 agattttcct gagcctgatt gaccaactaa tgcaatggtt ttaccctttt caattgttag
     7261 gttgatatta cgtaataccc atgcgttttg atatttaaaa ctcacatttt taaattcgat
     7321 cgaatgatta aatccagaaa tctcttttcc gtttacggtg tgtaatgaat tttgtgtgtg
     7381 caaaatctta tcaattcttt cgagcgatgc catgcctttt tgcacgctat aagcagcttt
     7441 tgatagttct tttacaggat tgataatgct gtagaaaatt aagatgtaat aaataaatac
     7501 agatgcagtt aatgcttcgg aattgctcgt taaaataaga ttgccaccgt aacacaacac
     7561 ggttgctatt accacagtac ccataaattc gctcattgga tgtgctaata aataccgacc
     7621 ctgaatttta ttgagtgtgc ctcgtaagcg attgtttagt gctacaaatc gtgaaatgat
     7681 ttttttttcc gcattgaatg ctttaataat gcgtaatcca cccaatgttt cctctatttg
     7741 ggaggttaat tctcctgtaa gaattgaccc ttcgagcgat tttcgtttaa gcgattttcc
     7801 tacctgtcca ataaaatatc cacttattgg caatagtacc attacaaata acgtaagttc
     7861 ccaacttatt gtaaacataa cgataatgta agtaagaatc atcaaggggt ttttaaacaa
     7921 catatccagc gaactcatga ttgaagcttc tacttcattt acatctaagg tcatacgtga
     7981 catgatatca ccttttcgtt cttcgttaaa aaaaccaata ggtaaatgca caatttttgc
     8041 aaaaatcata tttcgcaagt cgcgtaatac ccctgtgcgt atggatacca taaagaaact
     8101 acctaggtag gctgttgcaa ctttcaaaaa tgtcattaat atgagaaaag aagagaggat
     8161 aataagtacg gttaacgctc cacttttgtc aattaaatcg gttgtgtaat aatataaatt
     8221 atttttcagt acatcaaaca attcgctcat tggcacattg tgtgtgcttt gtaattcata
     8281 tactacggct tttgtattaa acaatatttc gagtatcgga ataataagtg caaaggagaa
     8341 caaactaaaa atagtagata gtaaattgaa caaaacattt agccctacat attttttgta
     8401 tggagggata aatcgtttca gtaaaataat aaatcgtttc atttatacag tatggtttat
     8461 tataatccag tataattatg cggtgtaata gctcgcaatt ctgcttttac agattcgctt
     8521 acttgcaaag tctcgataaa tgaagcaata gattcggcat taacctcggt attggtgcga
     8581 gttaattctt ttagtgtttc gtatggtttt ggataatttt ctctacgtaa gatggtttga
     8641 atcccttcgg ctactacatt ccaacaatta tctaagtctc tttttaagct tgcttcattc
     8701 aaaattaatt tactaagtcc tttgagagta cttttgatag caattaaatt atgagccatt
     8761 ggaaccccta cattgcgtaa aacagtcgag tcggtaaggt ctctttgcaa gcgtgatatg
     8821 ggtaatttgc ccgctaaatg ttctaaaata gcattggcaa tacctaagtt tccttctgca
     8881 ttttcaaaat cgattggatt cactttgtgt ggcatcgcag atgaaccaac ttcccctact
     8941 ttaatttttt gtttgaaata ctccatcgaa atgtatgtcc agaaatccct attcatgtct
     9001 atcagaatag tattgatacg ttttatggca tcgaaaagtg ctgctaaatt atcgtaatta
     9061 gatatttggg ttgtatatgg ctctcgctct aagcctaaat aatttttcac aaaagaatta
     9121 ccaaagtcaa tccaattgat agtaggatag gctacatggt gagcgttaaa atttcctgtt
     9181 gctccaccca tttttgctga aataggagtc tgttttaaca acacaatttg ttgctctagt
     9241 cggcttacaa ataccctaat ttctttgcct aaacgtgtag gagatgctgg ttgtccgtga
     9301 gttttagcca gcatagggat gtgttcccat ttcgttgcta attgctccaa ttcatctacc
     9361 aattgctgta aaacgggcaa atacacagtg tataggcagt ctttaattga taatggaaca
     9421 gctgtattgt taatatcttg ggatgtgagt ccgaaatgaa taaattcttt ataatcagat
     9481 agcccaattt catcaaattt atctttaata aaatattcta ctgcttttac atcatggttg
     9541 gtgatgcttt ctgtgtcttt tactttttga gcatcagcta ctgaaaaaga ggtgtatata
     9601 gagcgtagaa ctggaaaaac ctttttttct atgttgttaa gttgaggtaa aggaagttcg
     9661 cacaaagcta taaagtattc gatttctact tgtactctgt atctaattag tgcgtattca
     9721 gaaaaatagt ttgtaagttc gtcaactttg gaacggtatc taccatcaac gggtgagata
     9781 gcagttagta aatttaagtt agtcatataa atagataatt aatagaagtg caaaggtaca
     9841 attatttaca cataattaca atatatccga tggtaagata tctgatattg gtgtaaaaaa
     9901 taaatgactt ggaaccattg tccaagtcat ttattacgta tatattttgt tttttataca
     9961 aacaagaaat aagtaagaat gtagagtggg agtaaaataa ccaaagaaaa tttaaaaata
    10021 tagccaaaga aactaggcat ttttatgcca ctactttcgg ctatagcttt taccataaag
    10081 ttaggaccat taccaatgta ggtcatagaa ccaaataaaa cagaccccat agatattgct
    10141 gttaataaca gttctggaac gcctgctact tctgctacag ctgctccttc ttgcactagt
    10201 cctaaggcta ctccatgcat cgctacagct gtaggagcat tatctaaaaa cgaacttaaa
    10261 ctacctgtca taaagtagaa ttgccagctt tcacttaatc caaaactagc cgcattttct
    10321 tttaaataca ataatgcagg taccatcgta ataaagatac ctaaaaataa agctgcaact
    10381 tcgtaaatag gagtccatga gaatcctttc agttcgcgtg ttttcttttt ggtaatatcc
    10441 atggatacta aaataaaccc aattaaaata atttcgcgta aatatttaat ccaaagagat
    10501 ccatgaggat tgcccatttc tggaatataa tttacattga caaaagctac acataaaatt
    10561 actccaatca gcaaaaagaa attcttacta cccccaatac gtaaaggttc tacgtttgca
    10621 ttatcgcttt ctattgcttc tggtttttct tttttataat atctcctatc taacaaatag
    10681 tataacccaa ttagaacggt acctataaat aaccattcag gaagcatatg taaaaaccaa
    10741 ccaaaatcag ctccacgtag atataatagg aataatggag gatctccgag tggagtaagt
    10801 aatcctcctg catttgctac ggcagcaata aaaaatagaa tggtatgcgt tttatagata
    10861 cgttggctat tggttgtaat taatgggcga attaagagca tggctgctcc tgttgtaccc
    10921 ataatagacg ctaacacgta cccaattgcc ataaataagg tgttaatttt tggtttggct
    10981 tgaatgtctc ccgtaagaaa taagccacca gaaataacgt acaaagatgt aagtaaaatt
    11041 ataaaaggaa cataatcgaa cattatttga tgttctaaat catggctaaa tccatttata
    11101 atcagaaaga taactgttgg tacactcagt aagaatgaaa ccaataattt gtttttgttt
    11161 tgctcccacc agtgttccca taaaagagga ccaatggcaa tcataatgag catcaatgca
    11221 aaaggggcag atgcccaaat tggcatagca acgtgctgtg caacatcaga aacatgttcc
    11281 atagaaattg aattttaagg taaaataatt tatttaaaaa atattgaaaa ctaatctaat
    11341 ttaagtacct ccataaacgc tttttgtggt acttctactg aaccaatttg cttcattcgt
    11401 ttttttcctt ttttctgttt ctccagtagt ttacgctttc gtgaaatatc gcccccataa
    11461 cattttgctg taacatcttt gcgtacagct ttaatggttt cgcgggcaat aatttttgaa
    11521 ccaatggcag cttgaatagc gatatcaaac tgctgacgag gaattaattc gcgtaatttc
    11581 tcgcacattc tacgaccaat tgtaaccgca ttatcaaagt gagtgagtga tgacagggca
    11641 tctactgatt ctccatttaa taaaatatct agttttacca attttgaagg acgaaatccg
    11701 ctgatatggt aatcaaacga agcatatcct ttggaaatgg acttcaattt atcataaaaa
    11761 tcaaagacta tttctcccat aggcatatcg taaattaatt cgatacgatt agatgaaata
    11821 taatcttgtt ttatcaattc tcctcttttt cccaaacaca aactcatgat agaaccaata
    11881 aaatccgttc gtgtaataat tgacgctcgt atataaggct cttctattct gtcaatcaat
    11941 gttatatctg gcattcctga tggattgtga acgtcgagca cttcgttttt acgcgtatat
    12001 attttatact gtacgttggg tactgtcgta attacatcca tgtcaaattc acgatcaagt
    12061 cgttcttgta caatctccat gtgcaacatg cctagaaatc cgcaacgaaa gccaaaacct
    12121 aaagcggctg atgactctgc ttcaaaagta agagacgcat cattcaattg taatttctcc
    12181 attgacgctc ttaggtcttc aaagtcctct gagtcaatag gatatatacc ggcgaaaacc
    12241 attggcttta cttcttcaaa cccctcaatg cctttttcgc aaggacgttt tacatgtgta
    12301 atagtgtcgc caacttttac ttctttagat accttaatgc ctgaaataat atatccaaca
    12361 tcaccacaac gtaattcttc tcttggttcc atacctaaac gaagcacgcc aatttcatca
    12421 gcaaaatact cttttccagt attaaagaat tttactaaat cgcctttgcg tatagttccg
    12481 ttttcaattt tcatataggt aataattcct ctgaaagagt tgaatacaga atcaaatacc
    12541 aaacattgca aaggagcttc tgggtctcct ttcggcggat gtatgcgttt gacaattgct
    12601 tgtaatattt cttgtatacc taaaccggtt tttccgctag cttttataat ttcatccgat
    12661 ttgcatccca gcaattctac aatttggtct tcaacttcct caggcatggc actatccata
    12721 tccattttat tcataatagg aataatttcc aaatcatgtt caatagccat gtataggtta
    12781 gagatggttt gagcctgaat gccttgtgta gcatctacaa tcaacaaagc accttcgcaa
    12841 gcagcaattg aacgagatac ttcatacgaa aaatctacat gtcctggagt gtcaataaga
    12901 ttgagtacat atttttctcc ttcatatatg tagttcatct gaatagcatg acttttgatg
    12961 gtaatgcctc tttctcgttc caaatccata ttatcaagta cttgagcttg catctctttg
    13021 cctgtaactg tgctagtctc ttcgagcaga cgatcggcta aggtactttt accatggtca
    13081 atatgggcaa taatacaaaa attgcgaata tttttcatcc gttctaatta ttattttaag
    13141 acccgcaaag atagtgaata attatagact aaaacataaa aaagagatga aaaaactgtt
    13201 tttttataaa tcaattttcg atgattattc tacatttatg tgcgtaaaat cacaaatttt
    13261 gactaatttt gtgagctatt tccaaaaaaa ggactatttg ctgtcttttt gatgatttat
    13321 taaactaaac cgaagataat tatgtttgaa aatttaagtg aacgcctcga acgatcgttt
    13381 aaaattctta aagggcaggg taaaattact gaaataaatg tagcagaaac attaaaagat
    13441 gtgcgtaaag ctcttttgga tgccgatgtt aactataaaa tagccaagac atttaccgat
    13501 acggttaaag aaaaagcttt gggaatgaat gtgctaacag caattaagcc caacgagttg
    13561 atggttaaaa ttgtgcgtga tgagttagcc ttgttgatgg gtggaacatc cgtggatatt
    13621 aataccaaag gttcgcctgc tgtaatttta atgtctggtt tgcaaggttc tggtaaaaca
    13681 accttctcgg ggaagttagc caaaatgctt aaatcaaaaa aaggaaaaaa ccctttgctt
    13741 gctgcttgtg atgtgtatcg tccagctgct atcgagcaat taaaagtgct tggcgaacaa
    13801 ataggcgttc ctgtttatag tgaaatagaa aacaaaaatc ccattcagat agctcaaaat
    13861 gccattgctt ttgctaaaaa gaacggacac gatttgttaa ttattgatac agcgggtcgt
    13921 ttggctattg atgagcaaat gatgaacgaa atagctgcca ttaaacaagc tattcaaccg
    13981 caagagattt tgtttgtggt ggactcgatg acgggtcaag atgctgtgaa tactgcaaaa
    14041 gaatttaacg atagacttga ttttacgggt gttgtactta caaaattaga tggtgatacc
    14101 cgaggtggag cggctttatc tattcgttcg gtagtggata aacctatcaa atttgtagga
    14161 acgggcgaaa aaatggaagc cattgatcag tttcatcccg aacgtatggc agataggatt
    14221 ttgggtatgg gtgatattgt gtcgttagta gaaaaagccc aagaacaata tgacgaagaa
    14281 gaggctcgta gggtagcgaa aaaaattgcc aaaaatcagt ttgatttcaa tgattttatg
    14341 ggtcaaattc agcaaatcaa aaagatgggt aacctaaaag atttggcagc aatgatacca
    14401 ggtatgggca aagccatgaa agatgttgat attaaagatg atgcgtttaa aggcattgag
    14461 gcaatgatac aatctatgac tcctgccgaa agagaaaatc ctcaattact gaatggtaca
    14521 cgtaaaatgc gtattgccaa tggtagtggt acttctatac aagaagtaaa caaattgata
    14581 aaacaatttg atggcatacg aaaaatgatg cgtatggtac aaacaaatgg taataaattc
    14641 ccgaaattca aataaaagaa ctatgcaact tattgacgga aaagcaatag cggatattat
    14701 taaacaagaa atagcagtag aagtagcaac tataaagagc atgggtcaac gacctccaca
    14761 tttggctgct attttggtag gacacgatgg tggtagcgaa acgtatgtgg ctcataaagt
    14821 aaaagcttgc gagcaatgtg gatttaattc taccctgata cgatacgaag ataccgttac
    14881 tgaaactgaa ttgttggcaa aaattagtga attaaataac gatactgcca tagatggatt
    14941 cattgtacag ttgcctttgc caaaacatat ttcagaacaa aaaattatcg aagccattga
    15001 ttatcgcaaa gatgtagatg gttttcatcc aattaatgta ggacgcactt ctattggatt
    15061 gcctgcattt gtgtcggcta caccagctgg tataatggaa ttgcttaaac gttatggaat
    15121 tgctactgct ggcaaacatt gtgtggtgct aggaagaagt aatattgtag gcaaacctgt
    15181 ggcaaattta atgattcaaa aggcgtatcc gggcaatgct acggttacgg tatgtcatag
    15241 ctattcggct gatttagtgg agcaatgcca aaaagcagat attataattg cggcattggg
    15301 tcaaccaaat tttgtaaagg gaaatatgat aaaagacggt gctgtggtaa ttgatgttgg
    15361 aacaacccgt gtgccatcta ctacaactaa atcgggcttc aaacttacag gtgatgtatt
    15421 gtttgatgaa gtagctccaa aatgttcgta cataactcca gtgcctggtg gagtaggtcc
    15481 catgaccatt gtgtcgttga tgcgaaatac cttattggct gctaaaaagg agttgtttaa
    15541 ataatctcga aagtattctc caaatactaa agccaagtac ttttcgtgct tggcttcaac
    15601 ttatcttttt attttcagtt tacacgtgct tgtataattc tatttaaggg aaaaaatatt
    15661 ctgatagata cctaattcta ttaaaatatt tgtacttgtt ttatactcaa gaatcactaa
    15721 ttaatattaa atcgctattt gtctgctaat aaattattat ttattttcat attattttat
    15781 ttatttgtta tattattttt atctttttgt ataaaatata atttgtttta tttttgtaaa
    15841 atacaaatgt atataaatat ttaaaaaata aaatataatg attaaatttg catcgttttt
    15901 ctagcaaaaa gaaaacaata attattaata actaacacac tatttgtttt attatgaggc
    15961 agaagaaagt tgcatggaag tacagagtcc cctttttact tttagctatc gtggcaatcg
    16021 tatctcttat acctgtagac cacgaaacat taattccatt aggaggagag ctattagaag
    16081 taaatcatgc agatacatca tggttattga tggcatcggc actagtatta ttaatgacac
    16141 caggtttagc ctttttttac ggcggtatgg tgcgttataa gaatttagta tctactttat
    16201 tacaaagttt tattgcatta ggtattataa gtgtgctttg gattgttgtt ggttttagtt
    16261 tggcttttgg cgaaagtgta ggcggtttta ttggcaatcc tacctcattt atgtttttca
    16321 agaacgtggg tttaacacct catcctgatt ttggcccaac tataccattt gcattgtttg
    16381 ctatgtttca acttaagttt gctattatta cccctgcact tattactggt ggattagcag
    16441 aaagggtaag atttacctca ttgatgttgt ttttaatact tttttcactg ttaatatatg
    16501 ctcctttggc tcattggact tggcacccag aaggattttt acgtaaatgg ggtgtgcttg
    16561 attttgctgg tggcactgta gttcatattt ctgctggttt tgcagcactt agtggagcaa
    16621 tttttttggg taaaagaaaa ggggttaata gcgaagctcg tcctacaaac atcccttata
    16681 ttcttttagg tacaggattg ttgtggtttg gttggtttgg ttttaatgct ggatcttctt
    16741 tagctgcaaa tgatatggca gtaagagctt ttgttaatac taatatttca tcggcaacgg
    16801 ctatgatgac atggttgttt gtggatggag ctatgggcaa gaaacgttca gcagcaggtg
    16861 cagcaattgg tgcggtagtt ggtttggtgg ctattacacc cgcagcaggt tttgtaactg
    16921 tagggcaaag tattttcttc ggttttgtag cctcattagt aagttataca gctgtgcaat
    16981 taaaatctaa aacaaaaatt gatgacacct tagatgtatt tccatgccac ggattaggcg
    17041 gtattgtggg tatgttactt acaggtgtat ttgcggtgga aaatggggtt ggagtggggt
    17101 taattcatgg taatgttact caatttatgt tttatttatt agcattatta attgtgggtg
    17161 tatttacttt tggtggttca tatctgctgt ataaagtaac gaatttaatt ttaccgatga
    17221 gggttactgc tgagcaagaa gaaataggtc ttgatattac acagcacgga gaagaggctt
    17281 ttgatttatc tactaaatag atagaaatag tcaatataga aatatatatc attgtcaatt
    17341 cttctgatgg tgatttcgct attcttattg aactttggaa attctacctc tttgtatatt
    17401 ttatactggc taccattaaa ataatacaca catgcatgac cacttgcgtt aaaagtaaaa
    17461 tataatttat cttcagattc tccataaatt gtaaacgctt cttcatacaa agcaggatta
    17521 attttttctt gatttgcaaa ttgcgttgaa tttattgtta tgttacattt aatttcccat
    17581 tgttctggta tcttcgaaat gcgtcctgga agtatattta aataccaact atctttaaat
    17641 actgaataga ttcttttccc atttatttct tcgagacgat aatttttatt tttttgtatc
    17701 aacaagggtt catcaaagtt ttcttctaag attacttttg aatcaagatg gtattctgga
    17761 tataagtatt tttctactct aatttgcgaa tacaagctaa ggggaaggca taataaaatg
    17821 agtagttttt ttcatagttt gtagttaatg tgtatgattt cttagtgcta tttcaattct
    17881 acaatagatg caaattgttt ttttctattt ttcaccatgt ttttagtaat actactttgt
    17941 acgaactata catggtaggc aaagttaaaa aaagaaaatt aagaaaagaa tattttaagg
    18001 aaaataatac aacaatagga agaattaatg ctcatcatta ggtgtttaat tgttgagttt
    18061 agtaaattga atagttttca ttgtgttcct atatacaaaa aaaaagacca tcatacaaaa
    18121 aaagatggtc ttaggttatg tttgagagct aggtgattaa cctaaatacc cttttaatag
    18181 tttacttttg ctactactac gtaacttgcg aatggctttt tcttttattt gacgtacacg
    18241 ttcacgtgtt aagccaaatt tatctccaat ttcctctaat gtcatttctt gacacgaaat
    18301 accaaaaaac gatacaatga tatttttctc tctttcgctt aatgtgttta atgctctatc
    18361 aatttcagtt gccaacgatt cgttaagcaa aaacctgtca gccacgggcg aatcgtcatt
    18421 aaccaatacg tcaagcaaac tattgtcttc accttctaca aagggagcat ctaccgaaat
    18481 atgacgacca gatactttca gcgtatccgt aattttatct acaggtatat ccaactcttc
    18541 agccaattcc tctggcgaag gacgacgttc attttcttgt tcaaatttgg aaaaagcttt
    18601 gttgatttta tttaatgatc ctacttgatt tagtggtaaa cgtacaatac gagactgttc
    18661 tgctaaggct tgtaatattg attgacgaat ccaccataca gcgtacgaaa tgaacttaaa
    18721 accacgggtt tcatcaaatt tttcagcagc tttgattaat cctaaatttc cttcattgat
    18781 taaatctggt aggctaagac cttgattttg atattgtttt gctacagata caacaaatcg
    18841 taaattggct ctagtaagtt tttcgagggc aattctatct ccttttttta tgcgttgagc
    18901 tagctcaact tcttcttcta ctgtaataag gtcttcgcgt ccaatttctt gtaaatattt
    18961 gtccagcgaa gcactttctc tattagtgat tgattttacg attttcagtt gtctcataat
    19021 gcgggcaaaa ttagtttgca aagatagaag aaaaaaacga attaacgaaa aaatagtgta
    19081 ctcattcttc ttctatatgt tattttatgt tatctcattt agtttttcta tcccaatttt
    19141 ctatttcatt ttcattaaag tgttgtttca ataacataat tgcgtatctt tgtattcaga
    19201 tagtaggtcg gtttgtcgcc gttcattgtg tgtgaaagga ggaaagtccg ggcaacgtag
    19261 agcaccatac ttcttaacgg gaagttgttc gtgagagcaa agtagtgtaa cagaaaataa
    19321 ccgccatgcc ttgtgtgtgg taagggtgaa aaggtgaggt aagagctcac cggtttgttt
    19381 ggtaacaagc agagccgtac gccttatggg ttgcaagacc atgtataccg acgcatgtag
    19441 ggctgctcgt ccgatgtcgg ggggtaggtt gctagaggca acaagtgatt gttgttgtag
    19501 ataaatgaca aacatttacg caagtaaata cagaacccgg cttatagacc tgctattttt
    19561 ttataatact tgtatgctat ggctagattt actacattga ttattttttt taaatcagcc
    19621 ttacattttt tatgtttcga catttggcgg ataacggatg gggaagttac ggcagctaaa
    19681 aatcgtattt ataccattat aaaagtattt gttatttctg ttcgtcgttt ttttgaagat
    19741 aaaatatttg caaagtcggc ggctctgacc tattatacct tgctttctgt tgttcctgta
    19801 cttgccttag taattgcgat tggtaagggc tttggtgtgc aatattatct tgagaatcaa
    19861 ttcgttgcga tatttccaag ccatagtact gttttgaaag aagctttttt cttagtcgat
    19921 tcatatattc agcaaattca gggtggtgtg ttttttggaa ttggttttgt attgttgttt
    19981 tggtctgtac ttaacatgtt ttccgaaatt gagattgctt tcaatgatat ttggcagata
    20041 aaaaaattgc gaaatacttt tcgtcgtttt gccgattatt tttccatgat gttaatactt
    20101 cctgtgttac tcattgtatc tagtggattg tcaatttatt tgaacgggac tattcaagat
    20161 acggtgcttt cttcgttgat tgtgcctgta tggtatggtt ttctcaaaat attacctttc
    20221 attattaata ttgttttgtt tacattaatg tatttatttt ttccaaatac aaaagtgagg
    20281 tttattgccg ctttatttgc cggagtagtc gctggtgtat tgtttcagtt attccaaatg
    20341 ttgtatatta gcggacaaat ttggatatct tattataatg ccatttacgg tagttttgca
    20401 gcattgccat tgcttttgtt atggttatgg gcgtcgtggt ctatcatttt atatggtgca
    20461 gaatttgctt tttctgtgca aaatattaaa aattacgaat ttgagtctga tgttaaaaat
    20521 atatctaggc gttacgaaaa ctttttgttt gttcttatca gttctgtcat tgttaagcgt
    20581 tttgccgaaa agcttcctgc aatgaatgca gaagaattat ctactcacta taatattcca
    20641 atacgattag tcaatcgcat tgtatctaag ttactagatg cagggattat tgttgagagt
    20701 atttctactg taaaaaaaac ggaggaaatt gtttatcagc cagctataga tatccaacat
    20761 ttaacgcttg cttatttatt tgagcaaata gatggattgg ggtctgagaa ttttaaaatt
    20821 gacattacac atgagcatgc ccaaccttgg caagctacct tatttgttca aaatagcaca
    20881 cagtcctatg cttcttctgt attattaaaa gatttataac agactttatt caaaaatacc
    20941 acgtttgttc aattgctttt ggtatgctct agctttccgc aaatgttcgt cgaatgtgcg
    21001 actaaagttg tgtgtaccag aaaaatcact ttttgcgacc atatatacat atgggtgttt
    21061 ggtgtaattg agtacagcat caatactttc ttgggtagga atacgaatgg gagcaggagg
    21121 tagtccttta tagatatatg tattgaatgg agagttgata gcaaggtgtc gttttagtat
    21181 ccgattaaga gaaaaattgc ccgtagcata tcgcacagta gggtctgctt gtaacaacat
    21241 gtcttttcgt aatctattga tatagagacc tgctaccatt ttcttatctt caaaattatt
    21301 tgtctcttct tctactatag aagctaaaat gtgaatctca gatggtgtta aatttatttt
    21361 cttggctaat tgttttctgt tatcattcca aaacgttgta tattcttttt ctatcaattg
    21421 tataatttct tcgattggcg tatcccatgt aatttctgca ttaaaaggta agaacaaaca
    21481 caatgaatta gtcggagtaa gttcgtattt ttttaataaa gtaggattgt gaaattgttt
    21541 cattaacgaa gttgagtcaa ccattaattg gcgactaaga ttacgtgcaa cttgttgagt
    21601 attacgggta gaaaccaccc taagaataat tttgctttgt aatccgtgag caattctatt
    21661 cagtagatac ctgttagata gattgggctc tatgtcatat tttcctgttt ttgggggcat
    21721 atcagcatat ttcagagcaa tcgcaagttt caaagaaaat gtactattaa caacctccat
    21781 ttctgttaac gtgtttatta catcactcca cgtttgttca ggatacacaa agagatactg
    21841 ttttttgtta ccattaccat ttaaattagg gagaataaag gcattaagca ttaaaccact
    21901 agcagttatt cctattactg ctactagtat aagtagtgtt ttatggttac gtaaatagtt
    21961 cattttcttt tttgtttttt acaaatatgt tgctttttta taaatattga ataagaatgt
    22021 ttgaaaatat caaatctaat gactacattt gcagtcgcaa aaccagttta gcgtttgcat
    22081 tggaagtgtg ggtgagtggc tgaaaccacc agtttgctaa actgacgtac gggaaactgt
    22141 accgggggtt cgaatccccc cgcttccgcg aaagtccttt gaaagaagga ctttttttat
    22201 gtttacctac t
//
LOCUS       DAOSRP010000011        22114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2534_length_22114_cov_4.974614, whole genome shotgun sequence.
ACCESSION   DAOSRP010000011 DAOSRP010000000
VERSION     DAOSRP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22114
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_22114_cov_4.974614"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PLJ82_01460"
     CDS             complement(<1..1331)
                     /locus_tag="PLJ82_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40209.1"
                     /translation="MYKQKLSFFTLPCLFCVLFCTLNLSVIYAKDKPILDTSYVYTIA
                     EIEIVRTQRKQEILPAQTLQGDMLSKLSSHSVADAVRYFSGVQLKDYGGIGGLKTINI
                     RSMGTNHMGVFYNGIQLGNAQNGQVDLGRFSLDNMESISLYNGQKSTVLQSAKDFGAA
                     GSIYLQTKTPTFEPNKTYNLIGNYKTGSFGLHNPSLVWQQKITSTISSVVSAEYTNAH
                     GRYPFTFRKVSADGTVAYDTTAIRKNADIEAIRTEFGLFGKTSNTNWHVQVYNYHSER
                     GLPGYIARNIYEHQQRLWDNNFFVQSSIKYSKNKYSLLGNIKYADDYTRYLNPDYTLQ
                     YIDNEYRQKEIYGSLAQSFHLFNCWEMGIATDLQWNTLDADLYNFAYPTRFTELVAIT
                     QSFKFPHVQLQANALGTFVQEHVQVGTSSNNKSMVTPSLFASVQPWLYIPF"
     regulatory      complement(1364..1572)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1611..2099)
                     /gene="nrdG"
                     /locus_tag="PLJ82_01465"
     CDS             complement(1611..2099)
                     /gene="nrdG"
                     /locus_tag="PLJ82_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOR40210.1"
                     /translation="MLRYLNYNIVFQEVPDEVTLAIHISQCPHRCKGCHSPILQQNSG
                     EYLNESVLDHLLHTYQNAITCICFMGGDVDPKELERLALYVKQHSNIQTAWYSGSVKI
                     PEFCNILAFDYIKIGPYIEHLGGLNSTTTNQIFYAVRNGTLINITNRFQKKQALSMLN
                     CV"
     gene            complement(2077..4167)
                     /gene="nrdD"
                     /locus_tag="PLJ82_01470"
     CDS             complement(2077..4167)
                     /gene="nrdD"
                     /locus_tag="PLJ82_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOR40211.1"
                     /translation="METCIIKRDGKKENFSIEKIKSAIRKAFLSVGSFATDEVITHIL
                     SRVNITNGISVEDIQNQVEIALMAERYYAVAKAYMLYRQKHLEDREVKEKLEFLMEYC
                     DAKNAATGSKYDANANVENKNLATLIGELPKSNFIRLNRRILTDKLKEMYGKEVADRY
                     IDLLNNHYIYKNDETSLANYCASITMYPWLIGGTNSIGGNSKAPTNLKSFSGGFINMV
                     FMVSSMLSGACATPEFLMYMNYFIQLEFGDDYYLEADKVVDLSKKQRTLDKVITDYFE
                     QIVYSINQPTGARNFQAVFWNISYYDRYYFESLFEHFVFPNGSKPHWESLNWLQKRFM
                     KWFNQERTKTVLTFPVETMAILSDNGDVKDKEYGDFAAEMYAEGHSFFTYMSDNADSL
                     SSCCRLRNEIQDNGFSYTLGAGGVSTGSKSVLTINLNRCIQHATRKGMHYLFFLEEVV
                     DLVHKVQVAYNENLKYMHEKGMLPLFDAGYINMDRQYLTIGVNGLVEAAEALDIEISD
                     NPHYLEFVQQVLGLIESYNKKYKTKGMMFNCEMIPAENVGVKHAKWDKRSGYKVNRDC
                     YNSYFYIVEDESLNIIDKFRLHGKKYIEHLTGGSALHMNLEEHLSKIQYQHLLRVAIQ
                     EGCNYFTFNIPNTVCNVCGHIDKNNLKTCPKCNSHDIDYLTRIIGYMKRVSNFSQSRQ
                     LEANKRHYATISQL"
     regulatory      complement(4304..4524)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4748..5365)
                     /locus_tag="PLJ82_01475"
     CDS             complement(4748..5365)
                     /locus_tag="PLJ82_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40212.1"
                     /translation="MDIRFVTQTYYLVVLMCICFLFSCTTQISTSHHHPPSWSEPVIF
                     GKKPNAIHYNYIPEASSQKTLAMVELIITHIPQEYEGQLFTLVGGGVDVGLHGPNATG
                     FSGTGVHFAGGNGNPPQTIEYKKDYTKQVENGSLHFTIYYTPTDIIQHWDSTASRYVP
                     MTEVTLTMHPLGEWNSIFHSTINPYNWCIAGVGKGNKLYLEISLE"
     gene            complement(5347..6687)
                     /gene="mgtE"
                     /locus_tag="PLJ82_01480"
     CDS             complement(5347..6687)
                     /gene="mgtE"
                     /locus_tag="PLJ82_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR40213.1"
                     /translation="MNSTNIHLQSLIEEKKWKALRLEIIDRDPIEVANIIDEASLADA
                     IIVFRLLTREQAKETFQYLSYDKQEYIIEGLAKNVNKLSDLLNDLDPDDRTAFFEELP
                     GQITQRLIQLLSPEERKIATRLLGYPEDSIGRLMTPEFVAVKPHFTIAQTLNHIREYG
                     RNSETLNVIYVVDHHWKLIDDIKIRTILLAKPEQTISELMDNHYVSLSAFDDQEVAIR
                     VFQDQDRIAIPVTDTEGLLLGIITIDDVMDVVEEETTEDFHKFGAFQDAIDNPLKAKV
                     SFLYKKRIFWLTALIFMNIFSGAAIAHFQTTIQSVISLLFFLPLLIASGGNAGAQAAT
                     LTIRSLAVGDVESKDWLHLVGKEFLVSLLLGITMALGVSLIASFRAPDIIAVVAITMT
                     LTVMVGSLIGLLLPFIFTRLKMDPASASAPLITSIADISGVLIYFTVARWILGL"
     gene            complement(6878..7474)
                     /locus_tag="PLJ82_01485"
     CDS             complement(6878..7474)
                     /locus_tag="PLJ82_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOR40214.1"
                     /translation="MCQFVDAIQYSNGEFKRIAYHQKRAEAAVKEYYPLSNIFSIMDY
                     LRQLELPTTGIYKLRICYTDTIQSYECIPYKKREIKELHLVETHIKSRKYKLADRSDY
                     TIANKFDLNNSDILFVRDGLLTDTSYANIALLKNGIWYTPRTPLIFGVNRASLLDQGI
                     IVEKDILEKNIPHYEKIAIFNAMIEFGELILSINAIHR"
     gene            complement(7458..8450)
                     /locus_tag="PLJ82_01490"
     CDS             complement(7458..8450)
                     /locus_tag="PLJ82_01490"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOR40215.1"
                     /translation="MQSYPFIDIQDAILRINLWAKQKKAFFFAFDYNLHHAIALLADE
                     IDAENILYKIGGYTNESMQNDSLPANLVWQPQPLSFADYSKKCTYVKEQIQLGNSFLV
                     NLTQPTPIQTNVSLQDLYQLTDAKYKLWVKNHFVVLSPEPFIRIQGLHISSFPMKGTI
                     DASIPNAEKIILENPKEEAEHATIVDLIRNDLSMIATDVHIKRYRYIEHIQSLQKHLL
                     QVSSEICGTLPSNYHENLGTMIRKLLPAGSISGAPKTKTLDIIQSAEGYDRNFYTGIC
                     GYFDGENLDSAVMIRFIEQQDSQLVYKSGGGITANSDIQTEYEELIQKIYVPIR"
     gene            complement(8466..9236)
                     /locus_tag="PLJ82_01495"
     CDS             complement(8466..9236)
                     /locus_tag="PLJ82_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOR40216.1"
                     /translation="MNNMHQTVKTLGLMLCMLIFSISASATIPNKPNPPRLVNDFAGI
                     FAPNELQQLEQYLVLVDDSTSNQIVVVTVNDLGNYDKAQFATELGQQWGVGDASFDNG
                     IVLLIKPKVGNQSGEVFIAVGYGLEAVIPDAIAKRIIEEVTIPYFRQNDYYGGALATV
                     QYLHKLAKGEIDVAREAEPDYTRFVIFLVIFILFIIIASNSKKNNTDGKGGTRHTSYG
                     GAPWIITGGSGGFGRSSGGGFGGFSGGGFGGGGAGGRW"
     gene            complement(9229..9675)
                     /locus_tag="PLJ82_01500"
     CDS             complement(9229..9675)
                     /locus_tag="PLJ82_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOR40217.1"
                     /translation="MNPKTFFSKADQLRIENAIVEAEKQTSGEIRLHIQSLTKGDVMD
                     AAVGVFYRLEMNKTEQRNGVLFYLSINDHQFAVIGDSGINKVVPSNFWDSIYEGIISY
                     FKQNLYADGLVWAISQAGIQLSSFFPLQKDDTNELSNEISFETKNE"
     gene            complement(9678..10256)
                     /locus_tag="PLJ82_01505"
     CDS             complement(9678..10256)
                     /locus_tag="PLJ82_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR40218.1"
                     /translation="MNKKLILPISIISIVVILVIWAFSTYNSIVTLDEAVPAQWAQVE
                     NAYKRRADLIPNLVSTVKGYAAHEQGTFEAVVEARAKATQITVDPSNLTPEKLKEYQA
                     AQGQVGSALGKLLMITENYPELKANQNFLELQAQLEGTENRISTERHRFNESTRMFNS
                     KIRRFPGNLFGFDTRPYFEATEAEQAVPTVSF"
     gene            complement(10346..12025)
                     /locus_tag="PLJ82_01510"
     CDS             complement(10346..12025)
                     /locus_tag="PLJ82_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOR40219.1"
                     /translation="MDTAENKKSLNFIESIVEKDLKEGKNDGRVQTRFPPEPNGYLHI
                     GHAKAICMDFGIAERFGGICNLRFDDTNPITEDVEYVDAIKHDIQWLGFQWNSIYYAS
                     DYFQQLFELAVRLIKEGKAYVDEQSSEEIAKQKGTPTTPGIESPYRNRPIEESLHLFD
                     KMNAGEIAEGAMVLRAKIDMSSSNMHFRDPIMYRIINTRHHHRTGDKWKVYPMYDYAH
                     GQSDFFEGVTHSICTLEFEVHRPLYDWFIDQLATTNYRPQQYEFNRLNITYTVMSKRK
                     MLQLVKEKLVAGWDDPRMPTICGLRRRGYTPSAIRSFINMIGYTKYEGTIDASLMEHA
                     IREDLKPKADRVCAILDPIKLIITNYPAHQLELLSTQNNPDNEQAGERTIQFGKELYI
                     ERSDYMENAPKDYFRLTEGKEVRLKSAYIVTCTGSKKDENGRVVEVYCTYDAETKSGT
                     EGSKRKVKSTINWVEIGSSIDAEIRLYDRLFAIEDPSKSDQDFRDLLNPDSLHILKGA
                     KVEAYLLNAKPMDSFQFLKHGYFTVDLDSKPNHLIMNRTVSLKDNTPKEKK"
     gene            complement(12091..12861)
                     /gene="lpxA"
                     /locus_tag="PLJ82_01515"
     CDS             complement(12091..12861)
                     /gene="lpxA"
                     /locus_tag="PLJ82_01515"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOR40220.1"
                     /translation="MTNIHPEAQIGANVQIGPFVHIDKNVVIGEGTVIASNVSILEGA
                     RIGKNCKIFSGAVIAAIPQDLKFRGEETIAQIGDNTTIRECVTVNRGTASKGKTIVGN
                     NCLLMAYTHVAHDCVVGNNVILGNATQLAGEVTIHDHAILSGGILVHQFCSIGSHVII
                     QGGSRITKDVPPFITAGRDPLSYAGINTIGLRRRGYSNEQIQSIQDIYRIIYASGLNH
                     TQAIEKLQQEMSPSAEKDEVIHFLQSSERGIIKGYFDK"
     gene            complement(12903..14288)
                     /locus_tag="PLJ82_01520"
     CDS             complement(12903..14288)
                     /locus_tag="PLJ82_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOR40221.1"
                     /translation="MSTKQKTLQQPFTLEGKGLHTGLHIKATFMPAPDNTGIVFKRID
                     IAEKPEIAAVAENVIATERGTVISKNEIQVSTIEHAMAALFASEIDNCIIELNGPELP
                     ILEGSAIQYIESIKKVGIQEQAEEKDYFTVQDKIEFTSDSGASYILLPDDKFSVHALV
                     SFNSPILSNQYANLDSLADFETEIAACRTFVFVREIEPLLKNNLIKGGDLDNAIVIYD
                     QEMPQAELDRLATLMNVPSMKVNNLGYINTKPLVYDNEPARHKLLDIIGDLALIGRPI
                     IGKIIATKPGHAGNTQLAKKISKELRRQETTPPIYNPEIAPVMDTNRIKTLLPHRWPF
                     LMVDKILSLKDKEIIGVKNVTCNETFFNGHFPEEPVMPGVLIVEAMAQVGGLLVLNSV
                     DEPARYSTYFLKIDNVKFRQKVSPGDTLIFKLILLTEIRRGCANMKGYAFVGKKIVAE
                     AEFMAQIVKNK"
     gene            complement(14290..15333)
                     /gene="lpxD"
                     /locus_tag="PLJ82_01525"
     CDS             complement(14290..15333)
                     /gene="lpxD"
                     /locus_tag="PLJ82_01525"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOR40222.1"
                     /translation="MELSAQQIAAYLHGEVVGDASVTVNTFAKIEEGKPHSLSFLANP
                     KYTHYIYETQASIVLVNKTFIPEKPINATLIKVDDAYKSLADLLTLVENNKPKKTGID
                     PLAYIAPSATIGQNVYIGAFVYIEEGVVIGDNCQVYPHTYIGEKSTIGKDSILYSGVK
                     IYHQCVIGNQCIIHASSVIGADGFGFARNEDGSYSKIPQSGNVVVEDNVEIGACTTID
                     RASMGSTFIRKGVKLDNQIQIAHNVELGENTAMAACSGIAGSTKVGKNCILAGMVGLA
                     GHLNVPDNTIFAAYTGVPNNIKEAGIYQGIPAIPVAQFRRASVVYKQLPELQKTIIAL
                     QKEIELLKAELKK"
     gene            complement(15433..16656)
                     /locus_tag="PLJ82_01530"
     CDS             complement(15433..16656)
                     /locus_tag="PLJ82_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR40223.1"
                     /translation="MSNNKKKIINDPVFGFINIPGTLLYNLLQHPYVQRLTRIKQLGL
                     SSYVYPGAQHTRFQHSLGAMYLMTEAIKTLKSKGISISQHETEAACAAILLHDVGHGP
                     FSHALEGMIIPHLSHEEITLRIMQKINKEYEGKLDLALEIFQNTYHKTFLHQLVSGQL
                     DMDRLDYLRRDSFFTGVTEGNIGSARIIKMLNIKNDTLVVEAKGIYSIENFLMNRRLM
                     YWQVYFHKTAFAIEKMLIKTIERAKIIAKQDSTLFGTPSLLFFLRQEDNLTNDSALDL
                     FLNLDDTDIMCSIKQWINHPDTILSTLSYGIIHRKLFKIEVSNEPFSKAKIEAKTTEI
                     AQHLGINHMDAAYFVSVNDVSINMYHNNEDSITILHKDGSIKPISQTSDMLDIRILSR
                     EVTKYYFSYYKITPE"
     gene            complement(16661..17497)
                     /gene="pyrF"
                     /locus_tag="PLJ82_01535"
     CDS             complement(16661..17497)
                     /gene="pyrF"
                     /locus_tag="PLJ82_01535"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOR40224.1"
                     /translation="MNRQELVENIQRKKSFLCVGLDTDITKIPEVLFDSCDDPMFVFN
                     KRIIDATADLCVAYKPNLAFYESLGLEGWDVLERTIDYIRTNYPDQFIIADAKRGDIG
                     NTSAMYARTFFGTMDFDAVTVAPYMGEDSVTPFLTYENKWVILLALTSNKGAFDFQFL
                     QDKETNERLFEKVLHTSTQWGNTDNMMYVVGATKAEMLQDIRAIIPNHFLLVPGVGAQ
                     GGSLQEVAKYGMNAECGLLVNSSRQILYASSGEDFAEAARLEALAVKQEMEQLLKDAH
                     LV"
     gene            complement(17515..18594)
                     /gene="prfA"
                     /locus_tag="PLJ82_01540"
     CDS             complement(17515..18594)
                     /gene="prfA"
                     /locus_tag="PLJ82_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOR40225.1"
                     /translation="MSTLLQKLEGLATRFEEVSTLITDPSVIADMKRFVKLNKEYREL
                     EKINGARIKYEQLIANIAEAKEILDKENDEELREMAKMELEQSSPLVATMEEEIKLLL
                     VPADPEDSKNAIVEIRGGTGGDEAAIFAGDLYKMYIRYCETKKWKVEVTNISEGTAGG
                     YKEVIFTVSGENVYGTLKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEAEEFDIEL
                     KESDVRVDTFCSSGAGGQSVNTTYSAIRLVHIPTGITVQCQDEKSQHKNKAKAMIELR
                     SRIYAIEHQKYLDEIGSKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLSAFM
                     DGDIQDCIDQLIIAENAERMKENEL"
     gene            complement(18613..19779)
                     /locus_tag="PLJ82_01545"
     CDS             complement(18613..19779)
                     /locus_tag="PLJ82_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOR40226.1"
                     /translation="MSNQRYMMRGVSASKEDVHKAIQHIDKGLYPKAFCKIIPDILGG
                     DPDFCNIMHADGAGTKSSLAYLYWKETGDISVWKGIAQDALIMNIDDLICVGAIDNIL
                     VSSTIGRNKLLIPGEVIAAIINGTEELLAELRELGIGVYGTGGETADVGDLVRTIIVD
                     STVTCRMKRADVIDNARIEGGDVIVGLASYGQATYEKSYNGGMGSNGLTSARHDVFAN
                     YLANKYPESYDASMPSELAYSGSLQLTDAVPNSPIDAGKLVLSPTRTYAPVVKQLLDT
                     MRNSIHGMVHCSGGAQTKILHFIDNLHVIKDNLFPVPPLFKTIQEESGTDWAEMYKVF
                     NMGHRLEVYLPKEYAQQVIDIAQSFGIDAQVIGRVETAEKKKLTINSEFGTFVY"
     gene            19964..20731
                     /locus_tag="PLJ82_01550"
     CDS             19964..20731
                     /locus_tag="PLJ82_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOR40227.1"
                     /translation="MNRMKKTMFIALLAMLLTSCAELLTIANQVIVTQPVAVSNTENI
                     AGLKSSLDVGIEKAVGMLSIENGFYNDAVLKVLLPPEAQPIVDNIKLIPGGQELVNKA
                     VLSLNRSAEDAVKEATPIFKKAILSMTIADATNILFGADNAATEYLRKTTYSDLSVAF
                     SPKVKESLGKPLVANVSTTESWKSLTSAYNQVANTPVGLIAGLKPVNVNLEQYVTEKA
                     LDALFVKVAEEEKAIRTDPVARINDILKRVFGQLDKK"
     gene            complement(20929..22065)
                     /gene="cydB"
                     /locus_tag="PLJ82_01555"
     CDS             complement(20929..22065)
                     /gene="cydB"
                     /locus_tag="PLJ82_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOR40228.1"
                     /translation="MLNSLSHFDLQQYWWLIMSLVGSILVFLLFVQGGQSLISTLGKT
                     ADERNMVVNSIGRKWEFTFTTLILFAAGMFAAFPLFYSTSFGGAYYAWKLFLIVFVIQ
                     AVSFEFRRKKGNLLGTKTYDTFLAINGYVAPFLLGAVVATFFTGSAFIRNEYNLSFWQ
                     TELYGIEILFEPTNLALGFAVLFLARILGAMYIINNTLENTIIERARKQVAINTVSFL
                     VFFLYFVYQLLVMDGYAVDPTSNQVYLEANKYLNNFLAMPISIVLFLVGVLLVIYGII
                     STWFMKSTKGIWFSGLGTVCTVLAIFFVAGFNNTSFYPSFIDLQSSLTIKNASSSHYT
                     LTAMTYISFLVPVVIAYIYFTWKAIDKHKITKEDIKEDAKHHVY"
BASE COUNT         6489 a         4120 c         3982 g         7523 t
ORIGIN      
        1 ttaaatggaa tatataacca gggttgaacc gaggcaaaca acgatggggt aaccatactt
       61 ttattgttag atgaggtacc cacttgtacg tgttcttgta caaacgtgcc caaggcattc
      121 gcctgaagtt gtacgtgtgg aaacttaaac gattgtgtaa tggcaactaa ttctgtaaaa
      181 cgagtagggt aggcaaagtt atataaatca gcatctaagg tattccattg caggtcggta
      241 gctatgccca tttcccagca gttgaacagg tgaaaagatt gtgctaagga gccgtatatt
      301 tctttttgac gatactcatt atcaatgtat tgcagggtgt aatctgggtt taaatagcgt
      361 gtgtaatcat cagcgtattt tatgttacct aataatgaat atttattttt tgagtattta
      421 atagaacttt gaacaaagaa attattgtcc caaaggcgtt gttggtgttc gtaaatattg
      481 cgagcaatgt atcctggcaa acctctttcg gagtgataat tatacacttg cacgtgccaa
      541 tttgtattgc ttgttttacc aaacaaacca aattctgttc taatagcctc tatgtcagcg
      601 ttttttctaa ttgctgttgt gtcgtaggct acagtaccat cagcactcac ttttctgaaa
      661 gtaaatggat accttccgtg agcattggta tattcggcac ttactacgct actaatggta
      721 gaggtgattt tttgttgcca taccagcgat ggattgtgca acccaaacga gcccgttttg
      781 taattaccaa taaggttgta ggttttgttg ggttcaaatg tgggtgtttt tgtctgtaaa
      841 taaatgctac cagcagcccc aaaatcttta gcagattgca aaacagtgct tttttgtccg
      901 ttatacaagc taatagattc catattgtcc aatgaaaatc ttcctaaatc tacctgtccg
      961 ttttgagcat tacccaattg aattccatta taaaacacgc ccatgtggtt ggtgcccata
     1021 cttcgaatat tgatggtttt taagccacca atgccaccgt aatctttcag ttgaacaccc
     1081 gaaaaatagc gtactgcatc tgctactgag tgtgaactca atttgctaag catatcgcct
     1141 tgtagtgttt gagcaggaag aatttcttgt ttgcgttggg tacgaacaat ttcaatttca
     1201 gcaattgtat atacgtaaga cgtgtcaagt ataggtttgt cttttgcata tataaccgat
     1261 aaattgagtg tacaaaatag cacacaaaac aaacagggta gcgtaaaaaa tgataacttt
     1321 tgcttataca ttgttttata agtcctttcc tcgagaacta tatgaattat gattaagcaa
     1381 aggcaggtct tctgacttac tcccgttatc gaacggcctt cccgtagcac ccaatgggtg
     1441 acagtggcaa aagatgtgtt cgaaacgtta taaagagctt acagcagcgg gactgttcag
     1501 gacttacacc tgattccctt ttaatcgttc tatgtgttga acatacaacg aaccattgcg
     1561 agggcaaaga tactattgtt ttttttagtg aacaacaatt ctattgaaaa ttatacacaa
     1621 tttaacatac taagtgcttg ctttttttga aatctatttg ttatattgat gagtgttccg
     1681 ttgcgaacag cataaaaaat ttggtttgtt gttgtagagt ttagtccacc cagatgttcg
     1741 atatagggtc cgattttaat ataatcaaaa gcaaggatgt tacaaaactc aggaattttt
     1801 acgcttcctg agtaccatgc agtttgtata ttactgtgtt gttttacgta tagggcaagt
     1861 ctttctaatt cttttggatc gacgtctcca cccataaaac agatacaagt aatggcgttt
     1921 tgatacgtat ggagtaggtg gtcaagcaca ctttcgttta aatattcccc actattttgt
     1981 tgtaggatag ggctatggca tcccttgcac cgatgaggac attgactaat atgaatagcc
     2041 aatgtaactt catcaggcac ttcttgaaaa acaatgttat aattgagata tcgtagcata
     2101 atggcgttta tttgcttcta attgtctgct ttgcgaaaaa ttacttactc gtttcatgta
     2161 tccaataatg cgtgttaggt aatcaatatc atgactgttg cattttggac aagtttttag
     2221 gttgtttttg tcaatatgcc cacatacatt gcacacagta tttggaatat tgaaggtaaa
     2281 ataattacat ccttcttgaa tggctacacg aagcaaatgc tggtattgaa ttttacttag
     2341 gtgttcttct aaattcatat gtagtgcgga gcctcctgtt aggtgttcaa tgtatttttt
     2401 accatgcaaa cggaatttat ctataatatt gagcgactca tcttctacaa tgtagaaata
     2461 gctgttgtaa caatctctat ttactttata accacttcgt ttgtcccatt tagcatgttt
     2521 tacacctaca ttttcggcag gtatcatttc gcaattaaac atcatgcctt tcgttttgta
     2581 ctttttgttg tagctttcta ttagtcccaa tacttgttgc acaaattcta ggtaatgtgg
     2641 gttgtcggat atttctatat ccaaagcttc ggcagcttct accagtccat tcacacctat
     2701 ggttaggtac tgacgatcca tgttgatgta tccagcgtcg aacaaaggga gcatcccttt
     2761 ttcgtgcatg tatttcaggt tttcgttgta tgccacttgt actttgtgta ctaaatctac
     2821 cacttcttca aggaaaaata agtaatgcat tcccttgcgt gtagcgtgtt gaatacacct
     2881 atttagatta atagtaagta cacttttcga tcccgtagaa acaccaccag cacctaaggt
     2941 atagctaaat ccattgtcct gaatttcgtt gcgtaaccta cagcaactgc ttaacgagtc
     3001 agcattatcg ctcatatagg taaaaaagga gtgtccttct gcatacatct ctgctgcaaa
     3061 atctccatat tctttgtctt ttacatcgcc attatcgctt agtatagcca tcgtttctac
     3121 gggaaaagtc aaaactgttt ttgtgcgttc ttgattaaac catttcataa accgcttttg
     3181 taaccaattt aatgattccc aatgtggttt actaccgttt ggaaatacaa aatgttcaaa
     3241 taaactttca aaatagtaac ggtcgtaata cgaaatattc caaaatacag cttgaaagtt
     3301 tctggcacca gtaggttgat tgatagagta cacaatttgc tcaaaataat cggtaattac
     3361 cttatccaaa gtacgttgct ttttcgataa gtcaaccacc ttgtctgcct caaggtaata
     3421 atcatcgcca aattctagtt gaataaaata attcatgtac atcaaaaatt cgggagtggc
     3481 acaagctcca ctcagcatgc tcgataccat aaacaccata ttgataaaac ctccgctaaa
     3541 ggattttaag ttggtggggg ctttagagtt tcctccaatt gagtttgtac caccaatgag
     3601 ccacggatac atggtaatgc tggcacaata attggctaag ctcgtttcgt cgtttttgta
     3661 tatgtagtgg ttgtttagca agtcaatata ccgatcggct acttctttgc catacatttc
     3721 ttttagttta tctgtaagta tgcgacggtt gaggcgaata aagtttgatt ttgggagttc
     3781 gccaataagg gttgctaagt ttttattctc cacattggca tttgcgtcat atttgctgcc
     3841 tgtagctgca tttttagcat cgcaatattc cattaaaaat tccagttttt cttttacttc
     3901 cctgtcttcc aaatgttttt ggcgatacag catgtaggct ttggctacag cataatagcg
     3961 ttctgccatc aaggctattt ctacttggtt ttgaatgtct tctaccgaaa taccattggt
     4021 aatgtttaca cgacttaaaa tgtgtgtaat tacttcatct gtggcaaaac tgccaacaga
     4081 taaaaaagct ttacgaatag cacttttgat tttttcaatc gagaaatttt cttttttccc
     4141 gtcacgttta atgatacatg tttccatact gtatataatt ttgtaaataa ttgctactaa
     4201 cttgcacgat gagacagtag ggatacaaac agacgaatgg aaagtttctg aataaatata
     4261 gatttatcgg agaactatcc tgttgccttt cacccgaaag cccagataat aatttacata
     4321 cggcaggtct tctgacttac tctcattttg aaaagccttc ccattctaca aatatgaaca
     4381 gtggcataag ggtttcaaaa tttctttaca ggtcttcttt aagttgtaaa aaaagagttt
     4441 acagctacgg ggatagttct cgaattgcac gagattccca ttttaatcca tcaataatat
     4501 ggaaccgtta gtgcaacaaa aatacagtat ttttttagaa agttatccaa aaaaggaaag
     4561 aatagttatt aacagaaata taaaaaatct aattatttaa tataatattt tggttgccaa
     4621 tagattgtat tttatattat ttatattaat ttctgaaaat gcaaataata gaaaaataaa
     4681 gtagtagaaa taatgatttt gaggataatc taacttgcta attattttca atgtaaattc
     4741 tatttattca ctctaaactt atttcaagat acaatttatt tccttttcct acaccagcta
     4801 tacaccagtt gtatggattg attgtagagt gaaaaatact attccattct cctagggggt
     4861 gcatggtaag tgttacttct gtcattggaa catagcgaga tgctgttgaa tcccaatgtt
     4921 gaataatgtc tgtaggcgtg taataaatgg tgaaatgcaa actaccgttt tctacttgtt
     4981 tggtgtaatc ttttttgtat tcaatggttt gaggtggatt gccattccct cctgcaaaat
     5041 gaactccagt acctgaaaat ccagtagcgt ttggtccatg taaccctaca tctacaccac
     5101 cacccaccaa ggtaaatagc tgtccctcat attcttgagg tatgtgtgtg atgattaatt
     5161 ctaccattgc caaagttttt tgtgaagaag cttcgggtat gtagttatag tgtatagcat
     5221 tcggcttttt tccgaaaata actggttcag accaactagg tggatgatgg tgtgatgttg
     5281 atatttgtgt tgtgcaagaa aataaaaaac agatacacat caatacgaca agataatatg
     5341 tttgggttac aaacctaata tccatctagc aacagtaaaa taaattaata ctcccgatat
     5401 atctgctata gaggtaataa gaggagcact agctgatgca gggtccattt taagacgtgt
     5461 aaaaataaat ggcaatagta accctattaa actacctacc attaccgtta atgtcattgt
     5521 aatagcaaca acagcaataa tatcaggagc tctaaatgat gcaataagac ttacacctaa
     5581 agccattgta atacctaaaa gtaaggatac taaaaattct ttcccaacta aatgtaacca
     5641 gtccttactt tccacatcac ccactgctaa cgaacgtatt gttagggtag cagcttgagc
     5701 tcctgcgtta ccaccgctag caattagcaa gggtaaaaag aataacaaag aaataacact
     5761 ttgaatagtc gtttgaaaat gagctatagc tgcacccgaa aaaatattca taaaaattag
     5821 agctgtgagc caaaatatac gttttttata caaaaaagaa acttttgcct ttagagggtt
     5881 atcaattgca tcttgaaaag ctccaaattt atgaaagtcc tctgttgttt cttcttctac
     5941 cacatccatc acatcatcaa tagtgataat acctaacaat aatccttcgg tatcggttac
     6001 gggtattgca attctatctt gatcttgaaa cacacgaata gcaacttctt ggtcatcaaa
     6061 tgcacttaac gaaacatagt gattgtccat taattcgcta atggtttgct ctggtttggc
     6121 taataaaata gtgcgtattt tgatatcatc aattaatttc caatgatggt caacaacata
     6181 aataacattc aatgtttcag aattacgacc atattcacga atatgattaa gtgtttgagc
     6241 aatagtgaaa tgtggcttta cggcaacaaa ttctggggtc atcaaacgtc cgatactatc
     6301 ttcgggataa cccaataagc gtgtagctat tttacgctcc tcgggcgaaa gtaactgtat
     6361 taggcgttgt gttatttgcc ctggtagttc ttcgaaaaaa gcagttctat catcggggtc
     6421 aaggtcattt aataaatcgg atagtttatt tacatttttt gctaaccctt caatgatata
     6481 ctcttgtttg tcgtatgaca agtattgaaa tgtttctttt gcttgctctc gagtaagcaa
     6541 gcggaaaacg ataatagcat cagcaagtga tgcttcatct ataatgtttg cgacttcaat
     6601 agggtctcta tcaataatct caagacgtaa tgctttccat tttttttctt caataagaga
     6661 ttgaagatgg atattcgttg aattcatgta ttgtctcctc aacaatttat atctgtaaag
     6721 gagacggcag ccctttgcag ataggttata actttagtct actatgatac gggccttcat
     6781 ccataatgtg tgtatttttt ttaattgcgt acaaaagtaa acataaaaat tgttttaagg
     6841 ctattctttt ccattttttt tataaaaacg tacgacttta cctatgaata gcattgatag
     6901 aaagaatcaa ctctccaaat tctatcatcg cattaaaaat ggcaattttt tcgtagtgag
     6961 gtatgttctt ttcaaggatg tctttttcta caatgattcc ttggtcaagc aaagaggctc
     7021 tatttacacc aaaaataagg ggtgtgcgtg gagtatacca gatgccattt tttaacaatg
     7081 caatatttgc ataactggta tcggtcagta aaccatctct tacgaatagt atgtctgaat
     7141 tatttaaatc gaatttgttc gcaatagtgt aatcactcct gtcggctaat ttgtatttac
     7201 ggcttttaat atgtgtttcc actaaatgaa gttctttaat ttctcttttt ttatatggta
     7261 tacattcgta agattgaatt gtatctgtat agcaaatgcg tagtttgtat atcccagttg
     7321 ttggtaactc taactgacgt agataatcca tgatagaaaa aatattggat aaaggataat
     7381 actcttttac agcagcttcg gcacgttttt ggtggtaggc tattcgttta aactcaccat
     7441 tactatattg tatggcatca acgaattggc acataaatct tttgtattag ttcttcgtat
     7501 tcagtttgta tatcactatt agctgttata ccaccaccac ttttgtatac taattgactg
     7561 tcttgttgtt caataaatcg aatcataaca gcactatcca aattttctcc atcaaagtag
     7621 ccacaaatac ctgtataaaa attgcggtcg tagccctcgg ctgattgtat aatgtcaagt
     7681 gttttggttt ttggagcacc gctaattgaa cctgcaggta atagttttcg tatcattgtg
     7741 cctaaatttt cgtggtagtt actaggcaat gttccacata tttcggaact tacttgcaat
     7801 agatgttttt gcaatgattg gatgtgttca atataccgat accgtttgat atgtacatcg
     7861 gtggctatca tgcttaaatc atttcgaatt aaatcaacaa tagtagcgtg ttcggcttct
     7921 tctttggggt tttctaaaat tattttttct gcattaggaa tagaagcatc aattgttcct
     7981 ttcataggga atgaagaaat gtgtaagcct tgtatgcgaa taaatggctc gggagacaac
     8041 acaacaaagt ggttttttac ccaaagtttg tattttgcat ctgttagttg gtacaaatct
     8101 tgtaaggata cattcgtctg aatgggtgtt ggttgtgtta agtttaccaa aaacgaattg
     8161 cctaattgaa tttgctcttt tacatacgtg catttcttgc tgtaatctgc aaatgagagt
     8221 ggttgtggtt gccaaactag atttgcgggt agtgaatcat tttgcataga ttcgttggta
     8281 tacccaccaa ttttgtaaag gatgttttct gcgtcaattt cgtctgctaa aagtgcgata
     8341 gcgtgatgca ggttataatc aaatgcaaaa aagaacgctt ttttctgctt tgcccataaa
     8401 ttgatgcgta aaattgcatc ttgtatgtcg ataaatggat aagattgcat caatatgtat
     8461 acttgttacc agcgaccacc ggctcctccg ccaccaaaac caccaccact gaatccacca
     8521 aaaccaccac ctgacgaacg accaaaaccg ccactaccgc cagttataat ccaaggagca
     8581 ccaccatagc ttgtgtggcg tgttcctcct ttgccatcag tattattttt tttagaattg
     8641 ctggcgataa taataaatag gataaatata actagaaaaa ttacaaagcg tgtgtaatca
     8701 ggttctgctt ctcttgctac atcaatttca cctttagcta gcttatgtaa gtattgaaca
     8761 gtagccaatg ctccgccata atagtcgttt tgacgaaagt aggggatagt aacttcttca
     8821 ataatacgtt ttgcaatagc atcaggaata acagcttcta agccataacc cactgcaata
     8881 aatacttcgc ccgattggtt gcctaccttg ggtttaatta ataatacaat gccattatca
     8941 aatgatgcat ctccaacacc ccattgttgc cctaattcag tagcaaactg agctttgtcg
     9001 taattgccca aatcgtttac tgtaaccact actatttgat tggaagtaga atcgtctacc
     9061 agtaccaaat actgttctag ttgttgcaac tcgttgggag caaaaattcc tgcaaaatcg
     9121 tttaccaaac gaggaggatt gggtttattt ggaatagtag ccgatgcact tatcgaaaag
     9181 ataagcatac atagcattag tcccaaagtt tttactgttt ggtgcatgtt attcattttt
     9241 tgtttcaaaa gaaatttcgt tgctcaattc gttggtatca tccttttgaa gtgggaaaaa
     9301 gctagatagc tgaatgcctg cctgcgatat tgcccatacc aatccatctg catacaggtt
     9361 ttgtttaaaa taggaaataa ttccttcgta aatgctgtcc caaaaattgc ttggcactac
     9421 tttgttaata ccgctatctc caattacagc aaattgatgg tcgttaatag aaagataaaa
     9481 taacacacca ttgcgttgct ctgttttatt catctccaaa cgataaaaga caccgactgc
     9541 agcatccatt acatcccctt tggtgagtga ttggatgtgc aaacgtattt ctcccgaagt
     9601 ttgtttttct gcttctacta tggcattttc aatgcgtagt tggtcagctt ttgaaaaaaa
     9661 agtttttgga ttcattctta aaaactaaca gttggtacag cctgttcagc ttcagtagct
     9721 tcaaaatagg gtctggtatc aaatccaaac aagtttcctg ggaaacgacg tattttgcta
     9781 ttgaacatac gagtcgattc gttgaaacga tgccgttctg tactaattct attttcagtt
     9841 ccttcaagtt gtgcctgtaa ttctaaaaag ttttgattgg cttttagttc tggataattt
     9901 tcggtaatca tcaatagttt acctaatgca gagcctactt gaccttgagc tgcttggtat
     9961 tcttttagtt tctcaggagt taagttgctc gggtcaactg taatttgcgt tgctttagca
    10021 cgagcttcta ccacagcttc aaatgtgcct tgttcgtgtg cagcgtatcc tttcacggtg
    10081 cttactaggt tgggtattaa atcagcacga cgtttgtacg cattttctac ctgtgcccat
    10141 tgtgcaggaa cagcttcatc gagtgttaca atactattat acgtgctaaa tgcccatatc
    10201 acgagtatta ctacaatact gataatggaa attggaagaa tgagtttctt gttcatagtt
    10261 gataatattt tattcagata caaatgtact taaaattact tatttttcaa aatgccgaac
    10321 atatcactag gtgcattatt cgttattatt ttttttcttt aggtgtattg tcttttaaag
    10381 aaacagttct gttcattatc agatgattag gctttgaatc taaatcgact gtaaaatagc
    10441 catgtttcaa aaattgaaaa ctatccatgg gttttgcatt tagtaagtat gcttctactt
    10501 ttgctccttt tagtatgtgt aaggagtcgg ggtttagtaa atcgcgaaaa tcttggtctg
    10561 atttagatgg atcttcgatg gcaaataatc ggtcgtacaa gcgtatttca gcatcaatgc
    10621 tactgccaat ttcaacccag tttatggtgc tttttacttt tcgtttactt ccctctgtac
    10681 cacttttggt ttcagcatca tacgtacaat atacttctac aacacgtcca ttttcatctt
    10741 ttttagagcc tgtacatgtt acaatatagg cactttttag tcgaacttct tttccttcgg
    10801 tcaagcggaa ataatcttta ggtgcatttt ccatgtaatc agaacgttca atatacaatt
    10861 ctttgccaaa ttgtatggtg cgttcacctg cttgctcatt gtctggattg ttttgcgttg
    10921 acaataattc cagttggtgt gctggatagt ttgtaataat gagtttaata gggtctaaaa
    10981 tggcacatac tctatctgct ttaggtttta aatcttcacg tatagcatgt tccatcaatg
    11041 atgcgtcaat tgtgccttcg tatttggtat agccaatcat attgataaag ctgcgtatag
    11101 cacttggtgt ataccctctt ctgcgtaagc cacaaatggt tggcatacgt gggtcatccc
    11161 atcctgctac tagtttttct tttactaatt gtaacatttt tcgcttactc attacggtat
    11221 atgtaatatt taggcgatta aattcatatt gttgaggacg gtaattggtt gttgctaatt
    11281 ggtcgataaa ccaatcgtat aaagggcggt gtacctcaaa ctccaaggtg cagatagaat
    11341 gcgttactcc ctcaaaaaaa tcggactgac catgagcgta atcgtacatc ggatatacct
    11401 tccatttgtc gcctgtacga tgatgatgcc ttgtgtttat aatacggtac ataattgggt
    11461 cgcgaaagtg catgtttgac gatgacatat ctatcttggc acgcaatacc attgctcctt
    11521 cagcaatttc tccagcattc attttatcaa acaaatgtaa actttcttca atgggtctat
    11581 ttcgatacgg actttcgata ccaggtgtag taggggttcc tttttgtttg gctatttctt
    11641 cagatgattg ttcgtctacg taggcttttc cttcttttat taaccttaca gctaattcaa
    11701 ataattgttg aaaataatca gaagcatagt aaattgaatt ccactgaaat cctaaccatt
    11761 gaatatcatg ttttattgca tctacatatt ctacatcttc tgttatagga tttgtatcgt
    11821 caaaacgtaa attgcatata cccccaaatc gttcagcaat accaaaatcc atgcaaatag
    11881 ctttggcatg acctatatga aggtatccgt tgggttctgg tggaaaacgt gtttgcacac
    11941 gtccatcatt tttaccttct ttgaggtctt tttcaacaat actttcaatg aaatttaatg
    12001 actttttatt ttctgctgtg tccatagtaa caatcaagtt atattttttg aaaaaagctg
    12061 cctttttatt gacagcttcc ttcgtttgtt ttatttatca aaatatcctt taataatacc
    12121 tctttctgag gactgaagga agtgtattac ttcgtctttt tctgctgatg gagacatctc
    12181 ctgctgcagt ttttcaattg cctgtgtatg atttagtcca gatgcataaa taatacgata
    12241 aatatcttga atagattgta tttgttcgtt gctataccct cgtctgcgta atccaatcgt
    12301 gttgattcct gcatatgata atgggtctct tcctgcagtt ataaaaggag gcacatcttt
    12361 tgttatgcga gaacctcctt gaataataac gtgagaacca atactacaaa attggtgaac
    12421 caaaatacct ccacttaaaa tggcatgatc atgtatagta acttcacctg ctaattgagt
    12481 tgcattacct aagataacgt tgttgccaac cacacaatca tgtgctacgt gtgtgtatgc
    12541 cataagtaga caattattgc caacgattgt ttttccttta gaggcagtac ctctatttac
    12601 cgttacacat tcgcgaatgg tggtgttatc tccaatttga gcaatggttt cttcgccacg
    12661 aaattttaaa tcttgcggaa tagcggctat aacagctcca gaaaatattt tacaattttt
    12721 ccctatgcgg gctccttcta aaatgctaac gttagatgca ataacagtgc cttcgccaat
    12781 tacaacattt ttgtcaatat gcacaaaagg tccaatttgt acattcgctc ctatttgtgc
    12841 ttcggggtga atgtttgtca tactatgtat cattaatggt tgattgcgtt aaaatcgtta
    12901 tcttatttgt tttttacaat ttgagccata aattctgctt cggctactat ttttttacca
    12961 acaaaggcat agcctttcat attggcacaa ccccgacgaa tttcagtaag taatattaat
    13021 ttaaaaatga gggtgtcgcc aggagatact ttttgacgaa attttacatt atcaattttc
    13081 aaaaagtagg ttgaatatct agcgggctca tcaactgaat ttaataccaa taatcctcct
    13141 acttgagcca ttgcttctac tattaataca ccaggcataa caggctcttc tggaaaatgt
    13201 ccgttaaaaa aggtttcgtt acacgttaca ttttttacac caataatttc tttgtcctta
    13261 aggctaagga ttttatcaac catcaaaaag ggccagcgat gaggaagtaa ggttttaatc
    13321 cgattcgtat ccattactgg tgcaatttct ggattgtata taggaggtgt ggtttcttga
    13381 cgacgaagtt ctttgcttat tttttttgcc aattgggtat tgcctgcgtg tccaggttta
    13441 gtagcaatta tttttccaat aatgggtcga ccaataaggg caagatctcc tataatatca
    13501 agaagcttat gacgggcagg ctcattgtca tacactaaag gtttagtatt gatataacct
    13561 aggttattta ctttcatgga tggaacattc atcaatgtag ctaacctatc taattctgct
    13621 tgtggcattt cttgatcata aataacaatt gcattatcta aatcaccacc ttttataagg
    13681 ttatttttca gtaagggttc tatttcacga acaaatacaa aggttctaca agctgcaatt
    13741 tcagtttcaa aatcagctaa actgtccaaa tttgcatatt gatttgataa aataggtgaa
    13801 ttaaatgaca ctaatgcatg tacgctaaat ttatcgtctg gcaataaaat atacgatgca
    13861 ccactatcgc tggtaaattc aattttatct tgaactgtaa aataatcttt ttcttctgct
    13921 tgttcttgaa taccaacttt tttaattgat tcgatatact gaatggcact tccttcaagt
    13981 atggggagct ctggtccgtt cagttcaatg atacagttgt caatttcgga tgcaaaaaga
    14041 gctgccatag cgtgctcaat agtgcttact tgtatctcat ttttagaaat aactgttcct
    14101 cgttctgtag caatcacatt ttcggctact gcagctattt ctggtttttc agcaatgtca
    14161 attcgtttga aaactattcc ggtattatcc ggagctggca taaaagtagc tttgatgtgt
    14221 aagccggtgt gtaacccttt tccttctaaa gtaaatggtt gctgtaacgt tttttgtttt
    14281 gtcgacatct tactttttta attctgcttt tagtaattct atttcttttt gtaaggcaat
    14341 aatagttttt tgtagttcag gtaattgttt atatacgact gaagcacgtc taaattgtgc
    14401 tacaggtatg gctggtatgc cttggtaaat gcctgcttct ttaatattat ttggcacacc
    14461 agtataagcc gcaaaaatgg tgttatcggg cacattcaag tgtccagcta acccaaccat
    14521 accagctaaa atgcaatttt ttcccacttt ggtagagcct gcaatgcccg aacatgctgc
    14581 catagcagta ttttcgccca actctacatt atgggctatt tgtatttgat tgtctaattt
    14641 aacgccttta cggataaatg ttgagcccat actagctcgg tcaatggtgg tgcatgcacc
    14701 aatttctaca ttatcttcca ctactacatt gccactttgt ggtattttcg agtagcttcc
    14761 gtcttcgttg cgagcaaagc caaagccatc tgcaccaatt accgaagagg catgaattat
    14821 gcattgatta ccaataacac actgatggta aatttttacg cctgaatata aaatgctatc
    14881 tttcccaata gtgctttttt cgccaatgta ggtatgagga tatacttggc aattatctcc
    14941 aataaccact ccttcttcaa tatatacgaa tgctccaata tatacgtttt gtccgatagt
    15001 tgctgaggga gcaatgtatg ctaatggatc aatacctgtt tttttgggtt tattgttttc
    15061 gactaaggta agtaaatctg ctagtgattt gtaggcatca tctactttta taagagtcgc
    15121 attgattggt ttttctggta taaatgtttt atttaccaac acaatacttg cttgggtttc
    15181 gtaaatatag tgcgtatatt tagggttagc taaaaaactc aacgaatgag gttttccttc
    15241 ttcaattttt gcaaaagtat ttactgtaac ggaagcgtca ccaacgactt ccccatgtag
    15301 atatgcagct atttgctgag cggacaattc catataggca caaattttgg caaaggtaca
    15361 tattttttac aaatgaagag ctaatttttt atcagaaagt agtaacattt tacaacattt
    15421 tgtttatttt ttctattcag gtgttatttt gtaataacta aaatagtatt tcgttacttc
    15481 tctagataaa atgcgtatat ccaacatatc cgaagtttga cttatgggtt taatactacc
    15541 gtctttgtgt aaaatagtaa tactatcttc gttgttgtga tacatattaa ttgatacatc
    15601 atttaccgac acaaaataag cagcatccat atggtttata cctagatgtt gtgctatttc
    15661 tgttgttttt gcctctattt tcgctttgct aaatggctcg ttactaactt ctattttaaa
    15721 taattttcgg tgaataatgc catagctgag agtagataaa atcgtatcag ggtggtttat
    15781 ccattgcttt atagagcaca taatgtcggt atcgtcgagg tttaaaaata aatctaacgc
    15841 actatcgttg gtcaaattat cctcttgacg taaaaaaaag agcaacgatg gggtgccgaa
    15901 tagcgtgctg tcttgcttgg caataatttt cgctctttcg atggttttga taagcatttt
    15961 ttcgattgcg aaagctgttt tatgaaaata tacttgccaa tacatcaggc gtctgttcat
    16021 cagaaaattt tcgatagaat aaattccttt ggcttctact actagcgtat cgttttttat
    16081 gtttaacatt ttgataatac gtgccgaacc aatattgcct tcggtaactc ccgtaaaaaa
    16141 actatctcta cgcaaataat cgagcctatc catatccaat tgacccgaaa ctaactgatg
    16201 caaaaatgtt ttgtgatagg tgttttgaaa gatttctaag gctaaatcaa gtttcccttc
    16261 gtattcttta ttgatttttt gcatgatacg caaggttatt tcttcgtgac ttaggtgggg
    16321 aatgatcata ccttctaagg cgtgagaaaa cggaccatgc cccacatcat gcaataatat
    16381 ggcggcacaa gcagcttctg tttcgtgttg cgaaatgcta attccttttg atttgagtgt
    16441 tttaattgct tctgtcatca agtacatagc ccccaaggag tgttgaaagc gtgtgtgctg
    16501 tgccccagga tatacatacg atgataaacc aagttgtttg atgcgtgtaa gtcgttgcac
    16561 ataaggatgt tgcaataaat tatacaacaa tgtgcctggt atgttgataa aaccaaaaac
    16621 agggtcgtta attattttct ttttgttgtt agacacggca ttacactaaa tgagcatctt
    16681 tcaataattg ttccatttct tgtttcacag caagggcttc aaggcgagcg gcttctgcaa
    16741 aatcttctcc cgaagaggca tatagtattt gtcgcgacga atttactaac agaccacatt
    16801 cagcattcat accgtatttt gccacttctt gcaaactgcc accttgtgct ccaacacctg
    16861 gaacaagcaa aaaatgattc ggaataatag cacgaatatc ctgtaacatt tcggcttttg
    16921 tagcacctac tacatacatc atattatcgg tatttcccca ttgagtagat gtgtggagta
    16981 ctttttcgaa caagcgttca ttggtttctt tgtcttgtaa aaattgaaaa tcaaaagctc
    17041 ctttgttcga tgttaacgcc aacaaaatga cccacttatt ttcgtatgtt aaaaatggtg
    17101 ttacgctgtc ttcgcccata taaggagcta ctgtaactgc atcaaaatcc attgttccaa
    17161 agaacgtacg tgcatacata gccgaggtgt tgcctatatc gccccgttta gcatcagcaa
    17221 taataaattg gtcagggtaa tttgtacgaa tataatcaat ggtacgctct agcacatccc
    17281 aaccttctaa tcctaaactt tcgtaaaaag ctaagttggg tttgtatgca acgcataaat
    17341 cagccgtagc atcaataatt cgtttgttaa acacaaacat ggggtcgtcg caactgtcaa
    17401 aaagtacttc tggtatttta gtaatatcgg tatctaaacc aacacataga aacgattttt
    17461 tgcgttgtat attttcaact aattcttgtc tgttcatagt atcaaagtgt atcgttataa
    17521 ttcattttct ttcattcttt ctgcattttc ggcaattatc aactggtcga tacagtcttg
    17581 tatatcgcca tccataaaag cagataaatt atagatagta taattaatgc ggtggtcggt
    17641 aatacgtcct tgcgggtaat tgtaggtgcg aatttttgcc gaacgatcgc ctgttgaaac
    17701 cattgttttg cgtttcgacc caatttcgtc gaggtatttt tggtgttcaa tggcgtaaat
    17761 tctggaacga agctcaatca tcgctttggc tttgtttttg tgttgcgatt tttcatcttg
    17821 gcattgtacg gtaatacctg ttggtatatg caccaaacgg atagccgaat aggtagtgtt
    17881 taccgactgc cctccagctc ccgacgaaca gaatgtatca acacgtacat cgctttcttt
    17941 tagttctata tcaaattctt ctgcttcggg caatacagcc accgttgcag ctgaggtatg
    18001 cacacgtcct tgtgtttctg tttgtggtac acgttgtaca cggtgtacgc ctgattcgta
    18061 ttttaaggta ccatacacat tttcgcctga tacagtaaag attacttctt tgtagccacc
    18121 tgcagtacct tcgctaatgt tcgtaacttc tactttccat ttttttgttt cgcaatagcg
    18181 tatgtacatt ttatacaaat ctccagcaaa aatagctgct tcatcgcctc ctgttccgcc
    18241 tctaatttct acaatggcat ttttactgtc ttctgggtcg gctggcacga gcaataattt
    18301 gatttcttct tccattgtag caacgagtgg agaagattgt tctaattcca tttttgccat
    18361 ttctcgtagt tcctcgtcgt tttctttgtc aagaatttct ttggcttcag caatattggc
    18421 tattaattgt tcgtatttaa tgcgagctcc gttgattttt tcaagttcgc ggtattcttt
    18481 gttcagttta acaaaacgtt tcatatcagc tatcacagaa gggtctgtaa tcaatgtaga
    18541 aacttcttca aaacgagtag ctaaaccttc taatttttgc aataatgtgc tcatatatgt
    18601 ttttcgggct tgttaatata caaaagtgcc gaattcactg ttgatagtta attttttctt
    18661 ttcggctgtt tccactcttc ctataacttg tgcatctatg ccaaacgatt gtgcgatgtc
    18721 gattacttgc tgagcgtatt cttttggcaa atatacttct agtcggtgtc ccatattaaa
    18781 tactttgtac atttctgccc aatccgtacc actttcttct tgaatggttt taaataaggg
    18841 aggtactgga aataagttat ctttaataac gtgtagattg tctataaagt gcaatatttt
    18901 tgtttgagca cctcctgagc aatgcaccat gccatgaata gaattacgca ttgtgtctaa
    18961 caattgcttt actactggag catatgtgcg agtaggagag agtactaatt taccagcatc
    19021 tattggcgaa ttgggaacgg catctgtgag ttgtaacgac cctgaatatg ccaattcaga
    19081 gggcatactt gcatcgtaac tttcaggata cttattggct aaatagttag caaatacatc
    19141 atggcgagcc gaagttagtc cattgctacc cataccaccg ttgtaggatt tttcgtacgt
    19201 tgcttgaccg taagaggcaa gtccaacaat tacatcgcct ccctcaatgc gtgcattgtc
    19261 aattacatca gcacgtttca tgcggcaagt aactgtacta tctacgatga tggttcttac
    19321 taaatcgccc acatcagcag tttcaccacc tgtgccatat acaccaatac ctagttcacg
    19381 tagttcggct agcaactctt ctgtgccatt gataatagct gcgataactt cgcctggaat
    19441 aaggagttta ttgcgtccaa ttgttgaaga aaccaatata ttgtcaatag ctcccacaca
    19501 aattaaatca tcaatattca tgattaatgc atcttgtgca attcctttcc aaacggatat
    19561 atcaccagtt tctttccaat acaaataggc aagagaggat ttagtgccag ccccatctgc
    19621 atgcatgatg ttgcaaaaat cggggtcgcc acctaaaata tctggaataa ttttgcaaaa
    19681 agctttggga tataatcctt tatcaatatg ctgaatagcc ttatgcacat cttctttaga
    19741 ggcagatacg cctcgcatca tataacgttg attgctcatg gtaagcttgc ttttttttgt
    19801 tcggcaaagg taatcaaatt attacaaata gaaaagatta gcaactgcat actgaatcaa
    19861 gaattataaa caattaagta gtttttttct tttttgcact atttttcttt tgtacaatgt
    19921 gtatctttgc cctgtttagt aacacaaaaa atatattatg actatgaata gaatgaaaaa
    19981 aacgatgttt attgccctgt tagccatgtt gctaacttcg tgtgccgagt tattaaccat
    20041 tgctaatcag gtaattgtaa cacaaccagt agcagtaagt aatactgaaa atattgcagg
    20101 tctaaaaagt tcgcttgatg tagggataga aaaagctgtg ggtatgttga gtattgaaaa
    20161 tggattttat aacgatgctg ttttgaaagt tttgttgccc cccgaagctc aaccgattgt
    20221 tgataatata aagttaatac ctggtggtca agagttggta aacaaagccg tattgtccct
    20281 taatcgttcg gcagaagatg cagtaaaaga agcaacgccc atttttaaga aagccatctt
    20341 gagtatgacg atagccgacg caacgaatat tttgtttgga gccgataatg ctgctaccga
    20401 atacctgcgt aaaacaacat atagtgattt aagtgtcgct ttttcgccaa aggtgaaaga
    20461 gtcgttgggc aaaccattgg tagccaatgt atctactacc gaatcgtgga aatcgttaac
    20521 aagtgcatat aatcaagtag ccaatacgcc tgttgggttg attgcaggct tgaaacccgt
    20581 gaatgtaaat ttagaacaat acgttacaga aaaagcctta gatgctttgt ttgtaaaagt
    20641 ggctgaagaa gaaaaagcca ttcgtacaga tcctgtggca cgtatcaacg atattttaaa
    20701 acgtgtgttt ggtcagttag ataagaaata agcaactaat catcgtgtac aaaaagaaag
    20761 gcagctcgtt gtgaactgcc ttttttcgtt ttactctttt tctttactct tttccatcat
    20821 ttttagaaaa tagacaatca gtttgtaact tacaagtata aggatgggcc aactgacaag
    20881 ccataaaatg gatacaatat gttgcatagt attgttgttt tatgagggtt agtaaacatg
    20941 atgttttgcg tcttctttta tatcttcttt cgtaattttg tgtttgtcta tagctttcca
    21001 agtaaaataa atgtatgcta taacaaccgg cactaaaaag gatatatagg tcattgctgt
    21061 taatgtgtaa tgactggaag aggcattttt aatcgttaac gaactctgta aatcaataaa
    21121 agacggatag aaactcgtgt tgttaaagcc agctacaaag aaaatagcca aaacggtaca
    21181 tacagttccc aatcccgaaa accatattcc tttggtactt ttcataaacc atgtgctaat
    21241 aataccataa ataactaaaa gtacgcctac aagaaacaaa acaatactta ttggcatagc
    21301 cagaaagtta ttcaaatact tattggcttc caaatatacc tgattgcttg ttggatcaac
    21361 agcataaccg tccattacta aaagttgata cacaaaatac aagaaaaata ccaaaaaaga
    21421 cacggtatta attgctactt gttttcgtgc tcgttcaata attgtgttct ctagtgtgtt
    21481 gttgataata tacatagctc ccaatatgcg agctaaaaat agcactgcaa aacccaaagc
    21541 aaggttagta ggctcgaata atatctcaat gccatacaat tctgtttgcc aaaaagataa
    21601 attgtattcg ttgcggataa aagcactgcc agtaaagaat gtggctacca cagcacctag
    21661 taagaacggc gctacatacc cattgatggc taaaaatgta tcgtatgttt tagttcctag
    21721 cagattgcct tttttgcgtc taaactcaaa cgatacggct tgtatcacaa atacaatcaa
    21781 aaaaagtttc caagcataat aagctccacc aaacgaagtg gaatagaaca gtggaaatgc
    21841 cgcaaacata cctgctgcaa aaagaatcaa ggtagtaaac gtaaattccc atttacgtcc
    21901 aatggagttg accaccatat tgcgttcgtc ggctgttttc cctaaggtac taatcaatga
    21961 ttgacctcct tgtacaaaaa gaagaaaaac gagtatcgaa cctacaagcg acattataag
    22021 ccaccaatat tgttgtaaat caaagtgtga taatgaatta agcattttcc cctcctttcg
    22081 tattagttga ttcaggacct tttttgatag cagc
//
LOCUS       DAOSRP010000012        22073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2539_length_22073_cov_5.221546, whole genome shotgun sequence.
ACCESSION   DAOSRP010000012 DAOSRP010000000
VERSION     DAOSRP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22073
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_22073_cov_5.221546"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            720..1643
                     /gene="nadA"
                     /locus_tag="PLJ82_01560"
     CDS             720..1643
                     /gene="nadA"
                     /locus_tag="PLJ82_01560"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOR40229.1"
                     /translation="MTLQEKITKLKKEKNAIIMAHYYQTAEIQDIADFVGDSLALAQW
                     AASTDAAIIVLCGVNFMGETAKILSPNKKVLIPDMEAGCSLADSCKASDFEKFIQQHP
                     GHIVISYVNTTAEVKALTDVVVTSTNAHKIVDSFPKEQKIIFGPDKNLGNYINSVTGR
                     NMVLWDGACHVHEQFSLEKILDLKKQHPEAQILAHPECKKVVLTLADYIGSTSALLAY
                     TGTSDCNVFIVATESGILHKMKQQNPSKIFIPAPPNDSTCACNECNFMRLNTLEKLYN
                     CLLNETPEIQVDETIRQKAVKPILKMLEMSK"
     gene            1706..2617
                     /locus_tag="PLJ82_01565"
     CDS             1706..2617
                     /locus_tag="PLJ82_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOR40230.1"
                     /translation="MKETVAKPFLKWAGGKTQLIADIEKALPKNLLKDEFIYIEPFVG
                     SGAVLFWMLNNFPNLKKAIINDINEDLINTYKVIATYPHKLIQVLELFQNEFYLLEDK
                     IDEKKEYYYKKRTLYNTRKEDIITQAALFIFLNRTCFNGLYRVNKNNEFNVPMGSYKR
                     PTICDKENIVAVSKALQNVEILCGDFEQTLNYVTNNSLFYFDPPYKPLSETSSFNAYS
                     KDEFNDKEQIRLRNFCHKLEKLGHKWLLSNSDVKGKDVNNNFFDDIYSKFSILRVKAR
                     RNINANPEKRGELTELLITNTNLTITI"
     gene            2679..3134
                     /locus_tag="PLJ82_01570"
     CDS             2679..3134
                     /locus_tag="PLJ82_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40231.1"
                     /translation="MSDFLNLQKGYKVENGKVFFHGKLVGRIFKKHGFYKYLEELKIE
                     WKTLISKRLLPDDSIFVIIANTLFIIECKFQQVAGSVDEKLQTCDFKRKQYQKLLAPA
                     NIEVEYIYLLSDWFRKPEYKDVLNYIHSVHCFYFFEYIPLSKLGLPIPK"
     gene            3225..3476
                     /locus_tag="PLJ82_01575"
     CDS             3225..3476
                     /locus_tag="PLJ82_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40232.1"
                     /translation="MQVDIKNPTYIPNKIKSLYEYLVSVEESLTWYYYGLCVEIDPIF
                     DFNGDDALIRWVDINEGFNDKLIVHSLEQFKDNFKLTNA"
     gene            3469..4347
                     /locus_tag="PLJ82_01580"
     CDS             3469..4347
                     /locus_tag="PLJ82_01580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOR40233.1"
                     /translation="MLSPYFKSTDKNFYLLHGDTMDLLPKFDHKFDMVFADPPYFLSN
                     NGLSIQNGEIVSVNKGFWDKSEGFDFLNDFNRKWLSLVRDKMKDDATIWISGTMHNIF
                     SIGQILTELGFKILNVITWEKTNPPPNFSCRFFTHSTEQIIWARKSEKVPHYFNYELM
                     KQLNGDKQMKDVWKLPAIAPWEKSCGKHPTQKPLSVLTRLILASTRPNAWILDPFTGS
                     STTGIAANLANRKFLGIDQEEEFLIISKNRKLEIENLMLAANYKQKIRGFTNKMELEL
                     FLVEEPKVDYTKELRL"
     gene            4344..4886
                     /locus_tag="PLJ82_01585"
     CDS             4344..4886
                     /locus_tag="PLJ82_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40234.1"
                     /translation="MSLPNMLSRGKEPLAVFPVNGKYVLAVYKGKISEFDLLLKYRQR
                     DDSKKSGWSNIRTPKHIHWAVDVLLKMHSNEEETKRFLDFLTMVWDTQTVPLRTEVER
                     RNLLDVDRLLTEVNIEALNYESLADKGEYSIKFLILIARLLMIQEKTNMETAFMFKQL
                     IDALKAGQDIFKIISIATHR"
     gene            complement(4966..5844)
                     /gene="deoC"
                     /locus_tag="PLJ82_01590"
     CDS             complement(4966..5844)
                     /gene="deoC"
                     /locus_tag="PLJ82_01590"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOR40235.1"
                     /translation="MKDFKTLFSQYNCKLADDTVATKTSKIIADNFDTNNTSEVYKQL
                     FSFIDLTSLNSTDYEEQIQKFTEKVNTFPSDFPEMPNVAAICVYPSYAETVKNTLIED
                     VEIAAVAAGFPSSQTFLEIKVAETALAVHAGASEIDIVISIGKFLNKQYDEVFEEIEE
                     IKSACQAAHLKVILETGALAKAEEIKKASVLSMAAGADFIKTSTGKMSPAATLEAAYV
                     MCEAIKEFYEKTGKKIGFKPAGGIVTTKDAVEYYCIVKEILGDEWLTKDLFRLGASRL
                     ANNLLTAIYKEDISYF"
     gene            complement(5869..6321)
                     /gene="dtd"
                     /locus_tag="PLJ82_01595"
     CDS             complement(5869..6321)
                     /gene="dtd"
                     /locus_tag="PLJ82_01595"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR40236.1"
                     /translation="MRAVIQRVLKASVTINKEVHSEISQGLLLFVGIEDLDTEQDIDW
                     LVAKIVNLRIFSDDKGLMNKSVLDYGGDILVVSQFTLFAQTKKGNRPSFIRASKPDIA
                     IPMYQLFCQKLSNCLGKEVKEGIFGADMQVSLVNDGPVTIWIDTAHKE"
     gene            6547..7596
                     /locus_tag="PLJ82_01600"
     CDS             6547..7596
                     /locus_tag="PLJ82_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR40237.1"
                     /translation="MKHLFVGAFLLYVVSIFSQPPINYYQPAYNKSGTQLRTALQGII
                     DNHTIVSYDGLYDVYETSDNTADNKVWDMYSYCSWTHHNKTCGNYTKVCDCYNREHSV
                     PQSWFNKKSPMVSDAFHVYPTDGKVNNQRGNYPFGECTGGTTFSNGKGRAGSSTFSGY
                     SGTVFEPVDEYKGDFARTYFYMATRYAGACENWGNSVFGSVNLGLTNYAVNLFMKWHR
                     QDPVSPKEVTRNNAIYAHQDNRNPFIDYPELAEYIWGTKKSLSWNPIFLDEDPNFQYF
                     VYPNPVSDILYVHVDTTKNVQYVLYSVDKKVLASGVFDSNWSIDVRVLQSGMYVLQIY
                     VEGVSYSYKVLVNSN"
     gene            7613..8773
                     /locus_tag="PLJ82_01605"
     CDS             7613..8773
                     /locus_tag="PLJ82_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR40238.1"
                     /translation="MKFFITYITFFCVFFYVHASEFDSDVYYQEAEGKQQYELKTALH
                     KIIRGHTRVSYTPGIWQAYEKTDLRQNGTVWCIYTSCPFNYGVHPDYVEGYQCGSTGM
                     GYTECFCYSREHSFPKSFWGGGTALSDTVYTDVFHIYPASGRVNSVLRNDNVYANVGT
                     IIPKNNDDNPTSNGSKLGYSSVVGYDGQAFEIADEYKGDIARTYFYIATRYQHLIPQW
                     YTTSTGTKPNNARFILASNTSSVFQSWYINMLLAWHRQDPVSAKEIDRNNAVYTHVQN
                     NRNPFIDHPELVEYIWGNKQAEKWRQSTSIKTTTENLFSIYPASDTNFFFCDIKSDAN
                     IRFVIQDVSGKIIRRGVFSPPYQFFIKDLHKGIYIMHVKENGMLSETTFFVK"
     gene            8798..10909
                     /locus_tag="PLJ82_01610"
     CDS             8798..10909
                     /locus_tag="PLJ82_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR40239.1"
                     /translation="MRKLFFCFLFLLISIFSFYAESYSAYYLSAEGLSREHLKTALQR
                     RASKHQFLLYDELWNYFQQTDALDNNRVLDRYSSTEYVFSQSSQMDREHAFPKSWWGG
                     ATFYPVYSDLHHLYPSDRSANIAKSNNPLGKVSTNPSFSNGVSKVGPSVISGYSGTVF
                     EPADEYKGDFARAYFYVVTAYEQLFGIWDSPMLDNNTYPVFKTWAIDLLLEWHTNDPV
                     SQLEIDRNEQVYLVQQNRNPFIDYPQLVDYIWGNKQQDVFYTSSVITEPTIVSPWQDD
                     TIQFPAIFEDDEIEETVYIRGLNLSEGLSLSITDDANSYFSLAETTVSKTNAEQPNAY
                     PIKIKFSPTEVKEATAILSISGGGATPVSVVLSGTSLNRAVAKPEAQPISNVENNAAT
                     LSWTGVYKATNYFVDVYKNGLPKNTTVISEDFEGFYKTSFASNSQGAHSSDVSDSLDY
                     FMRTKEWAGDKIYHANKTAKLGTASAKGYITTPVLDLSHNNGNYTVKFDAKLWVGASE
                     KTTILILHNNVQVQEVTLTSDFQPFEFNFSNGTKESKITLTAKIASNNRFFLDNIRIE
                     QLQPEPIFVKENFDVGDVLFYDLYDLEYDTEYFYRVRANKEGDISANSNTVSFTLPFV
                     NSVLETNYANYDLFVQDGVIYFTNLENEAKLDFAIFSKIGKTILQDIVLPNQSSSYVL
                     SPGFYILKIGKEHFVKIMVSK"
     gene            complement(11051..12850)
                     /gene="uvrC"
                     /locus_tag="PLJ82_01615"
     CDS             complement(11051..12850)
                     /gene="uvrC"
                     /locus_tag="PLJ82_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOR40240.1"
                     /translation="MHENLKHIVDTLPELSGVYQYLNQKEEIIYVGKAKNLRKRVSSY
                     FSKSLTSKKTAVLLKNISNIVYTVVDSEEEALLLENNLIKQHQPRYNILLKDDKSFPY
                     ICIKNEPFPRIFKTRNVIKDGSEYYGPFTHMNTLKTLLDICKYQYTIRTCHLHLTKES
                     ILTHKFKVCLEYHIKNCKGPCVNKQTENDYLQQVNEVRSIIKGNIKEVCSSILKEINV
                     LANELRFEEAHILKQKLQILENYQSKWTVVNPSITHVDVFSIQENADESFVNYMNVVN
                     GCIVQAYTVTYKKQLDETKETILGLAIIEMRERFNSKTNEIIVPFVPDGKIKNAEFTV
                     PLRGDKKKLLELSIKNALQYKVDRLKQAEKLNPEQRTTRIVKQLQQDLHLNSLPLHIE
                     CFDNSNTQGTNPVSACVVFKSAKPSKKDYRHYHIKTVEGPDDFKSMQEVVYRRYSRLL
                     EENQPLPQLIIVDGGKGQLSSVYEILKKLDLDTKIAVIGIAKRLEEIFFPNDPIPLYL
                     DKKSETLKVIQHLRDEAHRFGITFHRKIRSKKQTVSSLDSIEGIGEQTKMILLKTFKS
                     VKRIKETPIEELEKIVGKAKAKKIADFYKNSIN"
     gene            complement(12853..13377)
                     /locus_tag="PLJ82_01620"
     CDS             complement(12853..13377)
                     /locus_tag="PLJ82_01620"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOR40241.1"
                     /translation="MTEEQVKKAIRDIPDFPSKGILFKDLTTAFKEPSCMKWFENELY
                     KRYKDKGITKIVGIESRGFVVAPILGNRLNIGFVPMRKKGKLPAETVEISYSKEYGID
                     TIELHKDSLTTDDIILIHDDLLATGGTMIAACKLAKKFNVKKIYVNFLVELDFLDGRK
                     GFDEDIEIDSLLHF"
     gene            complement(13456..15330)
                     /gene="mnmG"
                     /locus_tag="PLJ82_01625"
     CDS             complement(13456..15330)
                     /gene="mnmG"
                     /locus_tag="PLJ82_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOR40242.1"
                     /translation="MKFTYDIIVIGAGHAGCEAACAAANLGSQTLLITMDMNKIAQMS
                     CNPAIGGIAKGQIAREIDALGGFTGIVTDKTSIQFRMLNRSKGPAMWSPRSQCDRAKF
                     IETWRSVIENTANLYVWQDSAVSLVIENGAIAGVKTAMGVTFSCKAVVLTNGTFLNGL
                     LHIGKVQLNGGRVSEPASYGITEQLVAMGFSQGRMKTGTPVRIDARSVDFSQTTIQPG
                     ETDYHKFSYLDTPAHSLKQLPCYTLYTNEKVHQVLEKGLKDSPLYNGQIQSIGPRYCP
                     SIETKIVTFSDKTEHQLFLEPEGESTTEYYLNGFSSSLPIDIQLNALKEIPAFRNVHI
                     FRPGYAIEYDFFDPTQLRHTLETKLVKNLFFAGQINGTTGYEEAAGQGLIAGINAHMN
                     CVGGEKFILHRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADNRLTEKS
                     YTIGLATKERFELFKQKQTWCNEITEFIKNYSVKSSEIDSLLLSVGSTPLKQTTKLMD
                     ILLRPGISLQALIEALPSLQEQAEKITNRKQEIIEQIEISAKYEGYIARERLIADKLQ
                     RLENIKIRDKFNYDDISSLSTEAKQKLKKINPETIAQASRIPGISPSDINILLVLLGR
                     "
     gene            complement(15327..15758)
                     /gene="ybeY"
                     /locus_tag="PLJ82_01630"
     CDS             complement(15327..15758)
                     /gene="ybeY"
                     /locus_tag="PLJ82_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR40243.1"
                     /translation="MAVHFIFDDTKTLRLKKRMIAEWIKNVASGYGKSIGEISYIFCS
                     DNKILAINNEYLQHDYYTDIITFDYTEEQKIAGDIFISVDTVSSNAHIYTNTFSDEMA
                     RILIHGILHLCGIKDKTQQEEQNMRKAEDKALALLKEKILR"
     gene            complement(15767..17002)
                     /locus_tag="PLJ82_01635"
     CDS             complement(15767..17002)
                     /locus_tag="PLJ82_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOR40244.1"
                     /translation="MVLNYIWIGFFLIAFVVALFKLLVFGDMTIFPNIMNSTFEYAKM
                     GFELSLGLTGVMTLWLGVMKIGENGGAIKLLSKGISPLFNKLFPEIPKNHPVHGSMVM
                     NIAANMLGLDNAATPLGLKAMNELQELNKTKDTASNPMIMFLVLNTSGLTIIPISIMV
                     YRAQMGAADPSDIFIPILLSTFVSTLAGIIAVSIAQKINLFTKTILLYLGSITLLLTG
                     VIWGFSVLSSEKLNMVSSVAANIILFSIIIGFILLAIRKKINVYDSFIEGAKDGFSIA
                     VKIIPFLVAILVGIGVFRASGAMDSLMSGIAWLFTSIGLNANFVEALPVAFMKPLSGA
                     GARGLMIDAMNQHGVDSFVGRLACTFQGATDTTFYIIAVYFGSVGIRKVRHAVTCGLI
                     ADLAGLIAAIFIAYLFFHT"
     gene            17230..17979
                     /locus_tag="PLJ82_01640"
     CDS             17230..17979
                     /locus_tag="PLJ82_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOR40245.1"
                     /translation="MGFLGSFLGGALGFTVGGPIGAIIGVFLGSAISSNYGEKKKKNT
                     SQNDFKVSLLILIATVLKADGKVTKSELQETKAFLRANFGEEEALEALQMLKNILEQD
                     YDYKSICLQINQGLNYSSKLQLLQILFKISSADTIESSELRLLQTMATLMGISMGDFQ
                     SIQAIYSQHQHFHGQVKDTRKELDLAYQILEIDKNASDEEIKKAYRRMAMKYHPDKVS
                     ALCDEFKEIATEKFKAVKNAYELIKKEKGFA"
     gene            18007..20112
                     /gene="rnr"
                     /locus_tag="PLJ82_01645"
     CDS             18007..20112
                     /gene="rnr"
                     /locus_tag="PLJ82_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOR40246.1"
                     /translation="MKKKDIAINMMEVFKESPYKLFNYKQLASAINVKSQTDKRNVQQ
                     VLNELAALGSIEEMDKGKFRFSAKSKFVIGIIDKNSNGKTHLIPEDGGEKIFIADRNV
                     NRAMKNDTVKVLLYAARKNRQPEGEVVEIIKRSRETFVGVLSIKNNVAFVIIDNRILN
                     ADIYIPMDKLNGAKDGQKVLVKMTDWTPNAKSPFGEISDVLGEVGENNTEMHAILAEF
                     GLPYSYPEELERMAKNIDVGITPEEVAKRIDMRSITTFTIDPRDAKDFDDALSHRILD
                     NGNIEVGVHIADVTHYVKPHTPIDKEGFARAASVYLVDRTIPMLPEKLSNEVCSLRAN
                     EDRLCYSVIFELNDSADIVRYKIAKTVICSDKRFTYEEAQEIIEKKQGEFVSELLALD
                     TLAKKLRQKRFDLGAIAFDRYEVRFEIDEKGKPLSVYFKEQKDANKLIEEFMLLANKT
                     VAEHIGNVPKGKKAKTFVYRIHDEPNQEKLANFSLFIKRFGYKLKTTGKKKDIANSLN
                     HLLDEVTGKKEQNLIETLAVRSMAKAIYSTKNIGHYGLAFDHYSHFTSPIRRYPDMMV
                     HRLLTDYLEGGKSAPADKYEEYCEHCSDREQLAASAERASIKYKQVEFMKDKVGQVFD
                     GVISGITEWGIYVEIISNKCEGMIPIRDLDDDYYTYDEKNYCLIGTRLHKRYQLGDEV
                     AVLVAKANLEKKQLDFVLA"
     gene            20148..20582
                     /gene="dut"
                     /locus_tag="PLJ82_01650"
     CDS             20148..20582
                     /gene="dut"
                     /locus_tag="PLJ82_01650"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR40247.1"
                     /translation="MQINIVNKSKHDLPEYATPLSAGMDIRANLSEPITLLPLQRKLI
                     PTGLYIELPKGYEAQIRPRSGLALKKGITVLNTPGTIDADYRGEIGVILVNISDEPFV
                     VNDGERICQMVVAEHATVRWQQVEQLDETQRGTGGFGHTGKK"
     gene            20592..>22073
                     /locus_tag="PLJ82_01655"
     CDS             20592..>22073
                     /locus_tag="PLJ82_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40248.1"
                     /translation="MRFFSLTLIFITLVVPVAIATNNQEKLSTQVVDRSAQQPDFDYY
                     FAEALRQKHLGNTAESIDLFLKSYDINPTSAIVSFELGNLFLQQKADDMGFGYIKNAA
                     ELAPQNYFYQEKLALLYRSNGDFSKAIEVLERIQRLFPDKDEVLYELSQLYTQNGQHK
                     KSIQKLDLLEKRIGLNKAITYDKAVQYVLLKNIKQAHKEIDAMLKKTPNDHTLWILKG
                     DIEYERKKYGNAKKYFQKATDIQPESGYVYISWARYYEAIKDFSTSEEYLYKLFGAKD
                     ISFQDKLHYLEILTSYYSKRMDGIEKMHNLYTSLLQSHPMEYVAHTAYAEFLLQQKLV
                     PEAIEHFYTAALLHPSCLECWVYLLQYYTEKNENEKAYQIAKEAIEAVPNHPVLLFYS
                     GMLALMNDQKEDALVQLLEMIKFTDYNEPNYAEMNAAAHAMLVDLYLIYKKDYPKALE
                     QCDLALKLNPSNHVVMNNCAYTLALQNEQLEKAEKLSAQSLEAE"
BASE COUNT         7232 a         3608 c         3974 g         7259 t
ORIGIN      
        1 cgttcatata gatgtattgg cgataaaaat aagcaaaagc ataaaagatt tatcgcccat
       61 attgctttct atggaattta aaggtgtaat aaaatgttta ccaggtaata tttactcggt
      121 agtataaaca caaaagggac taataccttg ctagtattaa tcccttttta ttaaccttaa
      181 atcaaatact ttgaaaatca cactacaaaa gtatactgat ttataggata ttgtatcaaa
      241 tttaagcata atttgcataa ttatttacgc aagcgtttgc actgttagta atactgtact
      301 tattgtttaa aagctgttaa taaaatagag agtaaaaaat gataaaaaaa tttgtagtta
      361 taaattatgc tatttgttat acaaatattt tataaaacaa gaataatgtt atgaattttt
      421 aaaaaaagta atgaacaata aaaagagcgg ttattaacca taaaaaaatg aaaagtttac
      481 tacatttgta gttgtaaaca atatgttaat aaccttatca atacattttt tatcagcata
      541 ttatactgtt aattatctgt tgaaatactc tgtatattac accgtatttt aataatgcaa
      601 atttttagtg ttttatttat aaaccttatc aacaccctat tattcttatc attctttttt
      661 ataacttttt taaataaata aaagaataaa gaatactgtt gataataaca ataacgaaaa
      721 tgaccctaca agaaaagatt acaaaactca aaaaagaaaa gaatgccatt attatggctc
      781 actactacca aacagccgaa atacaagata ttgccgattt tgtgggcgat agtttggcat
      841 tggcacaatg ggctgctagt accgatgctg ccattattgt attatgcggg gtaaatttta
      901 tgggagaaac agctaaaata ttgtctccaa acaaaaaagt gctaataccc gatatggaag
      961 ctggatgctc attggctgat agttgtaaag caagtgattt tgaaaaattt atacaacaac
     1021 accctggcca tatagtgata tcttatgtaa atactaccgc tgaggtaaaa gccttaactg
     1081 atgtagtagt aacatctacc aatgcacata aaatagtgga tagctttccg aaagagcaaa
     1141 aaatcatttt tggaccagat aaaaacttag gaaactacat caatagtgtt acaggtagaa
     1201 acatggtgtt gtgggatgga gcttgtcatg tgcacgaaca gttttcatta gaaaaaatac
     1261 ttgaccttaa aaaacaacac ccagaagccc aaattttagc tcatccagag tgtaagaagg
     1321 ttgtgcttac cttagccgat tatattggtt ctacctctgc cctattagct tatacaggta
     1381 cttcagactg taatgtgttt atagtagcaa ccgaaagtgg tattttgcac aaaatgaaac
     1441 aacaaaaccc aagtaaaata ttcatacctg caccacccaa cgatagtacc tgtgcttgca
     1501 acgaatgtaa ctttatgcga cttaataccc tcgaaaaatt atataactgt ctactaaacg
     1561 aaacacccga aatacaagta gacgaaacca ttagacaaaa agccgtaaaa cccattttga
     1621 aaatgttgga aatgagtaag taggaggaat tggtattttt attattgaaa gattttaatt
     1681 acttttgaaa agttattaaa aaatcatgaa agaaacagtt gcaaaaccat ttttaaaatg
     1741 ggctggcggt aaaacacagc ttattgctga tatagaaaag gcattgccta aaaatttatt
     1801 gaaagatgaa tttatctata ttgaaccatt tgtgggcagt ggtgctgtac tattttggat
     1861 gttaaataac ttccctaatt taaagaaagc cattattaat gatattaacg aagatttaat
     1921 taatacatat aaagttatcg ctacttatcc acataaatta attcaagtat tggaattatt
     1981 tcaaaatgaa ttttatttat tagaagataa aattgatgaa aaaaaagagt attactataa
     2041 aaaaagaact ttatataata ctagaaaaga agatattata acacaagcag ccttatttat
     2101 ttttcttaat cgcacctgtt tcaatggctt atatagagtg aataaaaaca atgaatttaa
     2161 tgtaccaatg ggaagttata aaagacctac catttgcgat aaggaaaata tagtagcagt
     2221 gagtaaagct ttacaaaatg tagaaatttt gtgtggtgat tttgaacaaa ccctcaatta
     2281 cgtaactaat aattcattat tttattttga tcctccttat aaaccattga gtgaaacatc
     2341 aagttttaat gcttattcta aagatgaatt taacgataaa gagcaaatta gattaagaaa
     2401 tttttgccat aaactcgaaa aattaggaca caaatggtta ttgagtaatt cagatgtaaa
     2461 aggaaaagat gtgaataata atttctttga tgatatttat tcaaagtttt caattttaag
     2521 agtaaaagcg agaagaaata tcaatgctaa tccagaaaaa agaggtgagt taacggagtt
     2581 attaataaca aatactaatt taacaataac aatatgattt tggaggaaaa ggaggaggaa
     2641 atactaaaac aggactagtt tttgaaggcc atactgattt gtcagatttt ttaaatttac
     2701 aaaaagggta caaagttgaa aatggcaagg ttttttttca tggtaaatta gttggaagaa
     2761 tatttaaaaa acatgggttt tataaatatt tagaagaatt aaaaattgaa tggaaaactt
     2821 tgatttcaaa gaggttatta ccagatgata gtatttttgt aattattgca aatactttgt
     2881 ttattattga atgtaaattt caacaagttg cgggttctgt agatgaaaaa cttcaaacat
     2941 gtgattttaa acgtaaacaa tatcaaaaat tattagcacc agcaaatatc gaagttgaat
     3001 acatttatct tttaagtgat tggtttagaa aacctgaata taaagatgtt ttaaattaca
     3061 ttcatagtgt tcattgtttc tacttttttg aatatattcc gctatcaaaa ttagggttac
     3121 ctataccaaa ataataattt ataataacag atttaattgg gtaaaaaatt atatttcatc
     3181 attatttttc actatatttg tatagaaaaa tttaagatga aaatatgcaa gttgatataa
     3241 aaaatcccac atatattcca aacaaaatca aatcattata cgaatattta gtttctgttg
     3301 aagaatcatt aacatggtat tattatggat tgtgtgttga aatagatcct atttttgatt
     3361 ttaacggaga tgatgcattg ataagatggg ttgatattaa tgaaggtttt aacgataagt
     3421 taattgtaca ttctctcgaa caatttaaag ataactttaa gttgactaat gcttagcccc
     3481 tacttcaaat ccacagataa aaatttctat cttcttcacg gagatacaat ggatttgttg
     3541 cccaaatttg accataaatt tgatatggtt tttgctgatc caccttattt cttgtctaac
     3601 aatggtttaa gtattcaaaa tggtgaaatt gtaagcgtta ataagggatt ttgggataaa
     3661 tcagaagggt ttgatttttt aaatgacttc aatcgtaaat ggctttcatt agttcgtgac
     3721 aaaatgaaag atgatgcaac catttggatt agcggaacaa tgcacaacat tttttcaatt
     3781 ggacaaattt tgactgaatt aggttttaaa attttgaacg taattacgtg ggagaaaaca
     3841 aatccaccgc ctaacttttc ttgtcgtttt tttactcact caaccgagca gattatttgg
     3901 gcacgaaagt ccgagaaagt tcctcattac tttaattatg agttaatgaa acaactaaat
     3961 ggagacaaac aaatgaaaga tgtttggaaa ctccctgcaa ttgctccttg ggaaaaatcg
     4021 tgtggcaaac atccaacaca aaaaccgttg tctgttttga cacgacttat actagcctca
     4081 acgcgtccaa acgcttggat attggatcct tttacaggca gtagtacaac aggaattgca
     4141 gctaatttgg caaatagaaa gtttttagga attgaccaag aagaagaatt tttgattatc
     4201 agcaaaaata gaaaactaga gattgaaaat cttatgttag cggctaatta caaacaaaaa
     4261 atacgaggat ttaccaataa gatggaattg gaattattct tggttgaaga accaaaagtt
     4321 gactatacaa aagaattacg attatgagct taccgaatat gttatcaaga gggaaagagc
     4381 ctcttgctgt atttcccgta aatggtaaat atgtgttagc tgtttataaa ggaaaaattt
     4441 ctgagtttga tttactatta aaatataggc aaagagacga ttcaaaaaaa tcgggatggt
     4501 cgaacattag aaccccaaag catatacatt gggctgttga tgttttgcta aaaatgcatt
     4561 ctaacgaaga agagacaaaa cgatttcttg attttttgac aatggtttgg gacacacaaa
     4621 cggtaccatt acgtactgag gtagaaagaa gaaatctttt agatgttgat cgtttattga
     4681 cagaagtaaa catagaagcg ttaaactatg aatctcttgc agacaaaggt gagtatagta
     4741 ttaaatttct tattttaatt gcacgtcttt tgatgattca agaaaaaacg aatatggaaa
     4801 cagcgtttat gttcaaacag cttatagatg ctttaaaagc aggacaagat atttttaaaa
     4861 taatatcaat agcgactcac agataatgcc aataaataaa ttgaccatca aaacaaaaaa
     4921 aagcagaaca caaacgttct gctttttcaa tttatataat tcaaatcaaa aataactaat
     4981 atcttctttg taaatggcag ttaataggtt gttggcaagg cggctagcac ctaggcggaa
     5041 taagtctttg gtgagccatt cgtcgcctaa aatttctttt acaatacaat aatattctac
     5101 tgcatctttg gtggtaacaa tgccaccggc aggtttaaaa ccaatttttt tgccagtttt
     5161 ttcataaaat tctttgatgg cttcgcacat tacataggca gcttctaagg tagcggcggg
     5221 actcattttg cctgtagagg ttttaataaa atcagcacct gcagccatcg acaatacaga
     5281 tgcttttttt atttcttcgg cttttgctaa ggctcctgtt tctaaaatta cctttaaatg
     5341 agcagcttgg caagcggatt ttatttcttc aatttcttca aatacctcat cgtattgttt
     5401 gtttaaaaac ttacctatag agattacaat atcaatttca gaagcacctg catgcacagc
     5461 caaggctgtt tcagctactt ttatttctaa aaaagtttgt gacgaaggaa aaccagccgc
     5521 tacagccgct atttctacat cttcaatcaa ggtatttttt accgtttcgg catacgatgg
     5581 atatacacaa atagcagcaa cattcggcat ttcaggaaaa tcagagggaa aagtattcac
     5641 tttttctgta aatttttgta tttgctcttc gtagtctgtg ctattgagcg aagttaagtc
     5701 aataaacgaa aacaactgtt tatacacctc actcgtattg tttgtatcaa aattatcagc
     5761 tattattttg gatgttttag tagcaacagt atcgtcggcg agtttgcaat tgtattgcga
     5821 aaacagggtt ttaaaatctt tcatatacta tgtttattaa gttacttatt attctttatg
     5881 tgctgtatct atccaaatcg ttacaggtcc atcgttaacc aacgacacct gcatatctgc
     5941 tccaaatata ccttctttta cttccttacc caaacaatta ctcaatttct ggcaaaaaag
     6001 ttgatacatc ggtatagcga tatcaggttt agaggctcta ataaacgatg gtctatttcc
     6061 tttttttgtt tgagcaaata gcgtaaactg gcttaccacc aatatgtctc caccatagtc
     6121 tagcacagat ttattcatca atcccttgtc gtcagaaaat atacgtaggt ttacaatctt
     6181 agccactaac caatcaatat cttgctctgt atccaaatct tctataccaa caaatagcaa
     6241 caaaccctga gatatttcag aatgtacctc tttattgatg gtaacagatg cctttaaaac
     6301 acgttgtata acagccctca ttgtatataa ttttcttcac aaaattacaa tttatacgat
     6361 acttaatcaa atattaataa gttaattcgt tgatattttt tgtttttaca aatttaaaca
     6421 agtacatttg tgagtaatca gtacacgcgt aacaggtttg atacgtgctg taaatatgta
     6481 aaaaggaatc attttctgat aaaatggtga tatgatgcat ctaaacaagt tgtaatttta
     6541 aatgttatga aacatctatt tgtaggagca ttcttactgt atgtagtaag tatattttcc
     6601 caaccgccta tcaattatta ccaacctgca tataataaaa gtggaacgca attgcgtaca
     6661 gccttacaag gtattattga taatcatact attgtatcgt atgatggatt gtatgatgtg
     6721 tacgaaacaa gcgataatac tgccgacaac aaagtgtggg atatgtattc atattgcagc
     6781 tggacacacc acaataaaac gtgtggaaat tacaccaaag tatgtgattg ttacaatcgc
     6841 gaacacagtg ttccacaaag ttggtttaac aaaaaatcgc caatggtatc cgatgccttt
     6901 catgtttacc caacagatgg caaggtaaat aatcaacgcg gtaattatcc ttttggagag
     6961 tgcacaggag gcactacgtt tagtaatgga aaaggcaggg ctggctcttc aactttttca
     7021 ggatattcag gtacagtgtt tgagcccgta gatgaatata agggtgattt tgctcgtacc
     7081 tatttttata tggctacacg ctatgctgga gcttgcgaga attggggaaa tagtgttttt
     7141 ggtagtgtta atttaggctt aactaattac gcagttaatt tatttatgaa atggcatcga
     7201 caagaccccg tttcgcctaa agaggtaact cgtaataatg cgatatatgc acaccaagat
     7261 aaccgcaatc cgtttatcga ttatcctgaa ttagctgagt atatttgggg aacaaaaaag
     7321 tcattatcat ggaatcctat cttcttagac gaagatccaa attttcagta ttttgtgtat
     7381 cctaatcctg tttctgatat actctatgta cacgttgaca ctactaaaaa tgtgcaatac
     7441 gtattgtatt ctgtagataa aaaagtgctt gctagcggag tgtttgattc taactggagt
     7501 attgatgttc gtgttttaca gtcgggtatg tatgtgttac agatatatgt agagggtgtg
     7561 tcgtattcgt ataaagttct tgtaaatagc aattaatttt ttggcacatg caatgaagtt
     7621 ttttattaca tacataactt ttttttgtgt tttcttttat gtacatgcat cagagtttga
     7681 tagtgatgtg tattaccaag aagcagaggg aaaacaacaa tatgaactaa aaacagcatt
     7741 acacaaaata atacgcgggc atactagggt aagctataca ccaggtattt ggcaagccta
     7801 tgaaaaaaca gatttgaggc aaaatggaac cgtttggtgc atatatactt cgtgcccatt
     7861 taattatggc gtccatcctg attatgtaga gggctatcaa tgtggttcta cgggaatggg
     7921 gtatacggag tgtttttgtt atagcagaga acattctttt cctaaaagtt tctggggcgg
     7981 aggaacagca ttatctgata ctgtatatac cgatgtgttt catatttatc ctgcgagtgg
     8041 gagggtaaat agcgtgctcc gaaatgataa tgtatatgcg aatgttggaa caataattcc
     8101 aaaaaataat gacgacaatc ctacttcaaa cggttctaaa ttaggctatt cttctgttgt
     8161 aggatacgat ggtcaggctt ttgaaatagc agatgaatac aagggtgata tcgcacgtac
     8221 ttatttttat atagctactc gttatcaaca tcttataccg caatggtata ccacttcgac
     8281 gggaacaaaa ccgaataatg cacgctttat attagcatca aatacgagtt ctgtttttca
     8341 gtcatggtat atcaatatgc tacttgcgtg gcatagacaa gatcctgtat ctgcaaaaga
     8401 aatagataga aataatgccg tttatacaca cgtacaaaat aaccgtaatc cttttattga
     8461 tcaccctgaa ttggtcgaat atatttgggg aaataaacaa gctgaaaaat ggcgtcagtc
     8521 aacgtcaata aaaacaacaa cagaaaattt attcagtatt tatccagcaa gcgacactaa
     8581 tttttttttc tgtgatatta agtcggatgc aaatattagg tttgttattc aagatgtgtc
     8641 aggaaaaata attcgtagag gcgtgttttc tcctccatat cagtttttta ttaaagattt
     8701 gcataaaggt atttatatta tgcacgtaaa agaaaacgga atgttaagtg aaactacctt
     8761 ctttgtgaaa taatattttt ttaaagtaca caccattatg agaaaattat ttttttgctt
     8821 cttatttctt ttgattagta tcttttcttt ttatgctgaa tcatatagtg cttattattt
     8881 atcagctgaa ggattaagta gagaacattt aaaaactgca ttgcaacgaa gggcgagtaa
     8941 gcatcaattt ttgttatatg atgagctttg gaattatttt cagcaaacag atgcattgga
     9001 taacaatcgt gtgttagacc gttattcatc taccgaatat gttttttccc aatcatcaca
     9061 aatggatagg gaacatgctt ttccaaaaag ctggtggggt ggagccactt tttatccagt
     9121 gtattcagat ttgcaccacc tctatcctag cgataggagt gctaatatag ccaaaagcaa
     9181 taatccatta gggaaagtct ctactaatcc ttcattttct aatggggtaa gtaaagttgg
     9241 accctctgta atatcgggat attcaggaac cgtttttgag cctgcagacg aatacaaagg
     9301 cgattttgct cgggcttatt tttatgttgt aacagcctat gagcagttgt ttggcatatg
     9361 ggattctcct atgttagaca ataataccta tcctgttttc aagacttggg ctatcgattt
     9421 gttgttagag tggcatacta atgacccagt ttcgcagctt gaaatagata ggaacgaaca
     9481 agtatatctt gttcaacaga atagaaatcc atttatcgat taccctcaac tagtagatta
     9541 tatttgggga aataaacaac aagatgtatt ctacacttct agtgtaataa cagaacccac
     9601 catagtttct ccgtggcagg acgatacgat acaatttcct gctatttttg aagatgatga
     9661 gatagaggaa acggtctata taagaggttt gaatttatca gaaggcttat ctctttccat
     9721 cacagatgat gcgaatagtt atttttcttt ggctgaaact actgtttcta aaacaaatgc
     9781 agaacaacca aatgcatatc ctattaagat taaattttcg cctaccgagg ttaaagaggc
     9841 taccgctatt cttagtattt ctggaggagg agccacacct gtctctgttg ttttatcagg
     9901 tacatcctta aatagggcag tagccaaacc agaagcacag cccatatcaa atgttgaaaa
     9961 caatgcggct accttatcat ggacgggggt gtacaaggct accaattatt ttgttgatgt
    10021 gtataaaaat ggcttgccaa aaaataccac tgtgatttct gaggattttg agggttttta
    10081 taaaacaagt tttgcaagca attcgcaagg tgctcatagt tccgatgtgt ctgatagcct
    10141 cgattatttt atgcgtacaa aggagtgggc tggcgataaa atctatcatg ccaataaaac
    10201 cgccaaatta ggtactgctt ctgcaaaagg atatataact acacccgtgc tggatttatc
    10261 ccacaataac ggtaattaca cggttaaatt cgatgcaaaa ttatgggtag gtgcttccga
    10321 aaaaactact atccttattt tgcacaacaa tgtacaagtg caagaagtta cattaaccag
    10381 tgattttcag ccatttgaat ttaattttag caacggcaca aaagaatcga agattacatt
    10441 aacagcaaaa attgcatcaa acaaccgttt ctttttagac aatatacgta ttgaacaatt
    10501 gcaacccgaa cctatttttg taaaagagaa tttcgatgta ggagatgtat tgttttacga
    10561 tttgtatgat ttggaatacg atactgaata tttctatcgc gtaagagcaa ataaggaagg
    10621 agatatttca gccaattcga atacggtgtc ctttacgcta ccttttgtaa attctgtttt
    10681 ggaaacaaat tatgcaaatt atgatttgtt tgtacaagat ggggtaattt attttacaaa
    10741 cttggaaaac gaagctaagc ttgattttgc tattttttct aaaattggca aaaccatctt
    10801 acaagacatc gttttgccta atcaatcatc atcctatgtg ttatcgccag gattttatat
    10861 tctgaaaata gggaaagaac attttgttaa aattatggtg tcaaaataac agcaaatgaa
    10921 tgccgttttt ctgtacaagg tagcgattta aactaaatag ttaattctag agaagttaaa
    10981 ataagcttat ctgtcttgta atcaggataa tatagccatt ttttgcaatc gtaaaaaagg
    11041 ataaaatagc ctaatttata ctgtttttat aaaagtcggc tattttttta gctttcgctt
    11101 tacctacaat tttttctagc tcttcaattg gcgtttcttt tattcgtttt acacttttga
    11161 atgtttttag caatatcatt tttgtttgct cgccaattcc ctctatgctg tctaagctcg
    11221 aaactgtttg tttcttactg cgtatttttc tatgaaaggt gattccaaaa cgatgggctt
    11281 cgtcacgcaa atgttgtatg acttttaatg tttcggattt cttgtccaaa tagagcggaa
    11341 tggggtcgtt ggggaaaaat atttcttcaa gtcttttggc aattccaatt acagcaattt
    11401 ttgtatccaa gtcaagtttt ttcaatatct catatacgct gcttagctgc ccttttccgc
    11461 catccactat tattaattgg ggtaacggct ggttttcttc caataatcgc gaatatcgcc
    11521 tgtaaaccac ttcttgcatg ctcttaaagt cgtcaggtcc ttccactgtt tttatatggt
    11581 agtgtcggta atcttttttc gatggtttgg ctgatttaaa tacaacacat gctgatacgg
    11641 gattggttcc ttgtgtattt gaattgtcaa agcattcgat atgcaatggg agagagttaa
    11701 gatgtaggtc ttgttgtagt tgttttacta ttcttgttgt tcgttgttct gggtttagct
    11761 tttctgcttg ttttagtctg tcaactttat attgaagtgc attttttatc gacaattcga
    11821 gcagtttctt tttatcgcca cgtaaaggta cggtgaattc agcatttttt atttttccat
    11881 ctggcacaaa tgggacaata atttcgtttg ttttgctatt aaatcgttct cgcatttcga
    11941 taattgccag tcctaaaatt gtttcttttg tttcatcaag ttgctttttg tatgttactg
    12001 tgtatgcttg tacaatacaa ccgtttacaa cgttcatata atttacaaag ctttcatcgg
    12061 cattttcttg tatgctaaat acatcaacgt gtgtaatgct tggatttacc actgtccatt
    12121 ttgattggta gttttcaaga atttgtaact tttgtttcag tatgtgtgct tcttcaaacc
    12181 gtaattcatt tgctagtacg ttaatttctt tcagtatgga ggagcatact tcttttatgt
    12241 tgccttttat tattgagcgt acctcattaa cttgttgcag gtagtcattt tctgtctgtt
    12301 tgttaacaca gggacctttg caatttttta tatggtactc taaacagact ttaaacttat
    12361 gagtgagtat tgattctttg gttaggtgta aatggcatgt acgaatagtg tattggtatt
    12421 tgcaaatatc taacagcgtt ttcaaggtat tcatgtgggt aaacggtcca tagtattcag
    12481 agccgtcttt tatcacattg cgtgttttga aaatacgtgg aaatggttcg tttttgatac
    12541 aaatgtaagg gaaagactta tcatctttta gcaagatgtt gtagcgtggt tggtgttgtt
    12601 tgattaaatt gttttcgagc aataaggctt cttcttcact gtcaacaacc gtgtaaacta
    12661 tattggatat attttttaga agaacggctg ttttttttga ggtaagcgat ttggaaaaat
    12721 acgaagagac acgttttcgc aagttttttg ccttacctac gtatattatt tcttcttttt
    12781 gattcaagta ttgatacact cccgaaagtt ccggaagtgt atcaacaata tgtttgagat
    12841 tttcgtgcat agttagaaat ggagcagaga atctatttct atgtcttcgt caaatccctt
    12901 acgaccgtca agaaaatcca attcaactaa aaaattgacg tatatttttt ttacattaaa
    12961 tttcttagct aatttgcatg cggcaatcat tgtgccacca gtagctagca agtcatcgtg
    13021 aatcaagata atatcgtctg tagtaagaga atctttatgg agttctatgg tgtcgatgcc
    13081 gtattcttta gagtagctta tttctactgt ttctgctggt agtttgcctt ttttacgcat
    13141 aggaacaaag ccgatattga gcctatttcc taatatcgga gccactacga agccacgaga
    13201 ttctatacca acgattttgg tgattccttt atctttgtat cttttgtata gctcattttc
    13261 aaaccatttc atgcaggatg gttctttaaa tgctgtagtt aggtctttaa ataatattcc
    13321 cttacttgga aaatctggaa tgtctctaat tgcttttttt acttgttctt cggtcatatg
    13381 cttttgtttt tttttgtatg tttcacgtgg aacattgtgt tcttttaagg taaatttgtt
    13441 tcacgtgaaa caaatttatc gtcctaataa cactaataaa atattgatat cgctaggaga
    13501 tattcctggt attctgctag cttgggctat cgtttctggg ttgatttttt ttagtttttg
    13561 ttttgcttct gtcgataaag agctgatgtc gtcgtagttg aatttgtctc taatctttat
    13621 gttttctaat cgttgtagct tatcagcaat taaacgctct cttgcaatgt atccttcgta
    13681 tttggcagat atttctattt gctcaataat ttcttgtttt ctatttgtta ttttttctgc
    13741 ttgttcttgt aggcttggta atgcttcaat taatgcttgt aggctaatac ctggtcgcaa
    13801 aagaatatcc attagttttg ttgtttgttt taatggggtg gagccaactg agagtagcag
    13861 tgaatcaatt tcgctgcttt taacagagta gtttttaata aattctgtta tttcgttaca
    13921 ccaggtttgt ttttgtttaa acaattcaaa tcgttctttt gtggctaaac ctatggtata
    13981 tgatttttca gttaaacgat tgtctgcatc gtcttgtcgt agcaagatac ggtattcagc
    14041 acgcgatgta aacatgcgat aaggttcgtc aactcctttg gttaccaaat catctattaa
    14101 aacaccaata tatgcttcgt ctctatgtaa gataaacttt tctccaccaa cacaattcat
    14161 atgtgcatta atccctgcaa ttaacccctg tccagcagct tcttcgtacc ctgttgtgcc
    14221 atttatttga cccgcaaaaa acaggttttt taccagtttt gtttctaagg tatgtcttaa
    14281 ttgggtaggg tcgaaaaaat cgtattctat tgcatatcca ggtctgaaaa tatgtacatt
    14341 gcgaaaagct ggtatttctt tgagagcatt taattgaatg tcgataggca aggatgatga
    14401 aaatccattc aaataatatt ccgtagtact ttcaccttca ggttctaaaa agagttggtg
    14461 ttctgttttg tcagaaaagg ttacaatttt agtttcaata ctagggcaat aacgcggacc
    14521 tatactttgt atttgtccat tatatagagg ggaatctttt agtcctttct ctaatacttg
    14581 gtgtactttt tcgttggtat aaagagtgta gcaaggcaat tgttttaagc tatgtgcagg
    14641 cgtatctaga taggaaaatt tgtggtaatc tgtttcgcca ggctgaatag ttgtttggct
    14701 aaaatcaacg cttcgtgcgt ctatccgtac tggtgtaccc gttttcatcc ttccttgtga
    14761 aaaacccatt gctaccaatt gttctgtaat gccataactt gctggttctg aaactcttcc
    14821 tccgtttagt tgtactttgc caatatgtaa aagcccattt aaaaatgtac catttgttag
    14881 aacaacagct ttgcacgaaa aagtaactcc catagctgtt tttactccag ctattgcacc
    14941 attttctatt actaacgaca ccgcagaatc ttgccataca tacaaattgg cagtattctc
    15001 gataaccgat cgccatgttt ctataaattt agctctgtcg cattgcgaac gaggactcca
    15061 catagcaggt cctttggagc gattaagcat cctaaattgg atggatgttt tgtctgtaac
    15121 aatgcctgta aagcctccaa gtgcatctat ttccctcgct atctgtcctt tagctattcc
    15181 tccgatggcg ggattgcaac tcatttgtgc aattttgttc atatccattg ttatgagcaa
    15241 ggtctgcgaa ccaaggtttg cagccgcaca tgcagcttca catcctgcat gaccagcacc
    15301 aatcactatt atatcatacg taaatttcat cgtagtatct tttctttcaa taaggctaag
    15361 gctttatctt cagcctttcg catgttttgt tcttcttgtt gtgtcttatc ttttatgcca
    15421 cacaaatgca aaatgccatg tattaaaatg cgtgccattt catcgctaaa tgtgttggtg
    15481 taaatgtgtg cattgctgct tactgtatct acactgataa atatatctcc tgctattttt
    15541 tgttcttcag tgtaatcaaa ggttatgata tctgtatagt aatcatgttg taggtattcg
    15601 ttattgatag caagaatttt gttgtctgaa caaaaaatat agctgatttc accaatagat
    15661 tttccatatc cagaggctac attctttatc cattcagcta tcattctttt ttttaaacga
    15721 agcgttttag tatcatcaaa tataaaatgt actgccatag tgataattag gtatgaaaga
    15781 ataaatatgc aataaaaata gcagcaatta atccagctaa atcagcaatt aatccgcatg
    15841 ttacagcatg acgaactttt cgtataccta ctgacccaaa atacactgca attatataaa
    15901 acgtagtatc cgttgcccct tgaaaagtac atgccaaacg accaacgaat gaatctacac
    15961 catgctggtt cattgcatct atcattagcc cacgagctcc tgctccactc aagggtttca
    16021 taaaggcaac aggtaaagct tctacaaaat ttgcatttaa tccgattgat gtaaacagcc
    16081 aagcaatgcc cgacattaaa ctgtccatag ctcctgaagc cctaaacacg cctataccta
    16141 ccaagatagc tactaaaaac ggaataattt taacggcaat tgaaaaccca tctttagctc
    16201 cttcaataaa ggaatcgtac acatttattt ttttgcgtat tgccaataaa ataaatccaa
    16261 taattatgct aaataaaata atgtttgcgg ctacagaaga taccatgttt agtttttcag
    16321 aacttaatac agaaaaaccc caaatcactc ctgttagcaa aagtgtgata cttcctaaat
    16381 aaagtaaaat tgttttggta aataggttta ttttttgagc aatggatacg gctataatac
    16441 ccgccaatgt gctcacaaaa gtagagagaa gtatgggtat aaaaatatcc gacggatctg
    16501 ctgctcccat ttgtgctcgg tataccataa tagaaatggg aataatcgtt aatccagatg
    16561 tgttcaaaac caaaaacata atcatcggat tggatgcggt atccttcgtc ttatttaact
    16621 cttgcaattc gttcattgct tttaaaccaa gtggggtagc ggcattgtct agccccaaca
    16681 tgttggctgc aatattcatt accatagagc catgcacagg gtgatttttc ggaatttctg
    16741 gaaatagctt gttaaatagc ggactaatgc ctttggataa tagcttgata gcaccaccgt
    16801 tttcgcctat tttcatcact cccaaccaaa gggtcattac tccggttagt ccaagtgata
    16861 attcaaaacc cattttggca tattcaaacg tcgaattcat aatgtttgga aagattgtca
    16921 tatctccgaa cactagtaat ttgaacaatg ccactacaaa tgcaattaga aaaaatccta
    16981 tccaaatgta attcaatacc atattaagaa ttatttaacc cacaaagtta caaaaatatc
    17041 gcatgctttc gtatttttta gcaatttttt caaatcttct cctttcctgc ttttttatat
    17101 gtaaaattta actgaatagt atttctcttt ttgcggataa acataaaact tccatgtttt
    17161 taattaattt taaatcataa gttgacaaaa aatcaatacc tttgtaaatt aacaaaaata
    17221 tcgcgataaa tgggattttt gggtagtttt ttaggtggag cattggggtt tacggttggt
    17281 ggacctatcg gtgccattat tggtgtattt cttggctcgg ctattagttc taattatggt
    17341 gaaaaaaaga aaaaaaacac ctctcaaaat gattttaaag tatctcttct tattttgata
    17401 gccactgtat tgaaggctga cggaaaggta acgaaatcag aattacaaga aacaaaagct
    17461 tttttaagag caaattttgg tgaagaagaa gcactagaag ctctgcaaat gttgaaaaat
    17521 attttagaac aagattatga ttataaatca atttgtttgc aaataaatca agggttaaat
    17581 tattcatcca aattgcaatt gcttcaaatt ttatttaaaa tatcttctgc tgatacaatt
    17641 gaatcgtccg aattacgcct tttacaaaca atggctacac taatgggtat ttcaatgggc
    17701 gattttcaat ctattcaagc gatatactct cagcatcaac atttccatgg acaagtaaaa
    17761 gacacacgaa aagagcttga tttagcgtat caaatcttag aaatagataa aaatgcttcg
    17821 gacgaagaaa taaaaaaagc ctatcgtcgc atggctatga agtatcatcc cgataaggta
    17881 agtgctttgt gcgatgagtt taaagaaatt gcaaccgaaa agtttaaagc cgtaaaaaat
    17941 gcttacgaac tgataaaaaa agaaaaaggt tttgcttgat aaaggcaaac gataataaca
    18001 atacgtatga agaaaaaaga catagcaata aatatgatgg aggtttttaa agagtctcca
    18061 tataagttat ttaattacaa acagttagct tctgctatca atgttaaatc ccaaacggac
    18121 aaacggaatg tgcaacaagt gcttaacgaa ttagctgctc ttggttcgat agaagagatg
    18181 gataagggga agttccgttt tagtgctaaa agtaagtttg taataggaat aattgataaa
    18241 aatagtaacg gaaaaacaca tttaattcca gaagacggcg gagaaaaaat atttattgct
    18301 gatagaaatg taaaccgtgc aatgaaaaat gatacggtca aagtattgct ttatgccgct
    18361 cgtaaaaacc gtcaaccaga aggcgaagtt gttgaaataa ttaaacggtc tagagaaact
    18421 tttgttggcg tgttgtctat caaaaataat gttgcctttg tgattattga caatcggata
    18481 ttaaatgcgg atatttatat accaatggac aagctcaatg gggctaaaga tggtcaaaaa
    18541 gtattggtga aaatgaccga ttggacgcct aatgccaaaa gtccgtttgg tgaaattagc
    18601 gatgtgcttg gtgaagtagg tgagaataat accgaaatgc acgccatatt agcggaattt
    18661 gggttaccct attcttaccc agaagaatta gagcgtatgg caaaaaatat tgacgtaggc
    18721 attacgcccg aagaagtagc caaacgcatt gatatgcgtt ctattaccac ctttaccatc
    18781 gacccgcgcg atgccaaaga ttttgacgac gctctttcgc atcgcatttt agacaatgga
    18841 aatatagaag tgggcgtaca cattgccgac gtaactcatt atgttaaacc acatacgcct
    18901 attgacaagg aggggtttgc tcgagctgct tctgtctatt tagtagatag aaccatacct
    18961 atgttgcccg aaaaactgtc gaacgaagta tgttcgttac gtgccaacga agacaggctg
    19021 tgttattcgg tgatatttga gttgaatgat agtgcagata ttgtgcgata caagattgca
    19081 aaaacggtta tttgttccga caaacgtttt acatacgaag aggctcaaga aataatagaa
    19141 aagaaacaag gggagtttgt gtcagaattg ttggctttgg atacgcttgc taaaaaactt
    19201 cgacaaaaac gttttgatct tggtgctatt gcttttgata gatacgaggt gaggttcgaa
    19261 atagatgaaa aaggaaaacc attaagtgtg tattttaaag agcaaaaaga tgccaataaa
    19321 ttgatagaag aatttatgtt gcttgccaac aaaacggtag ccgagcatat cggaaatgta
    19381 cccaaaggaa aaaaagctaa aacatttgtg tatcgtattc acgacgagcc aaatcaagaa
    19441 aagctggcaa atttctccct tttcatcaag cgttttggtt ataaactcaa aacaacgggt
    19501 aagaaaaaag acattgccaa ttcgctcaat cacttattag atgaagtaac aggaaaaaaa
    19561 gagcaaaatt taatcgaaac attggctgtg cgttctatgg caaaagcgat ttattcgact
    19621 aaaaacatag gtcattacgg tttagctttt gaccattatt ctcatttcac ctcgcccatc
    19681 cgtcgttatc ccgatatgat ggtgcaccgt ttgctaacag actatttgga aggtggaaaa
    19741 tcagctcctg ctgataaata cgaagagtat tgcgaacatt gttcagatcg cgaacaactt
    19801 gctgcttcgg cagaaagggc ttctatcaag tacaagcaag tagagtttat gaaagataag
    19861 gtagggcagg tatttgatgg ggttatttca ggtattaccg aatggggcat ttatgtggaa
    19921 attatctcta ataagtgtga gggtatgatt cctattcgcg atttagacga tgattattac
    19981 acatatgacg aaaaaaatta ttgccttata ggcacacgct tacacaagcg ttaccagcta
    20041 ggcgatgaag tggctgtgct ggtagccaaa gccaatttag aaaagaaaca gctagatttt
    20101 gtattagcat aagtattacg taactatttt gaatatataa agaaatgata cagattaaca
    20161 ttgtaaacaa gtcaaaacac gatttacccg agtatgctac gcccttatct gctggaatgg
    20221 atatacgtgc taacctttct gagcccatta ctttgctacc tttgcaacgt aaattaattc
    20281 ctacgggttt gtatatagag ctacccaagg ggtacgaagc tcaaattcga ccacgaagcg
    20341 gacttgctct caaaaaaggc attacagtgc tcaatactcc tggcacgatt gatgccgatt
    20401 atcgcggtga aatcggtgtt attttggtta atatttcgga cgaaccgttt gttgtaaatg
    20461 atggcgagcg tatatgccaa atggtagtgg cagagcacgc aactgttcgt tggcaacaag
    20521 tagaacagct tgatgaaaca caacgtggta ctggcggttt tggtcatact ggtaaaaaat
    20581 agatgatacg aatgagattt ttttcactaa ctcttatatt cattacgctt gttgtgcctg
    20641 tagcaatagc taccaacaat caggaaaaac tatccacaca agtggttgat aggtcagctc
    20701 aacagcccga tttcgattat tattttgcag aagctttgcg acaaaagcat ttgggaaata
    20761 ctgccgaaag tattgatttg tttctcaaaa gttatgatat caatcctact agtgcgattg
    20821 tttcttttga attaggcaat ttgtttttgc aacaaaaagc cgatgatatg gggtttgggt
    20881 atatcaaaaa cgcagcagag ttagctcctc aaaactattt ctatcaagaa aaacttgcgt
    20941 tgctctaccg tagcaatggc gatttttcta aagccataga agtgttagag cgtattcagc
    21001 ggttattccc agataaagat gaggttttat atgagttatc tcaattgtat acacaaaatg
    21061 gacaacacaa aaaatcaata caaaaacttg atttgttgga aaaacgtata gggttaaaca
    21121 aagcaataac gtacgataaa gcggtacaat acgtattgtt gaaaaatata aagcaagcac
    21181 acaaagaaat tgatgcaatg cttaaaaaaa cgcccaatga tcatacttta tggatactga
    21241 aaggggatat agaatacgaa agaaaaaaat atggtaatgc aaaaaaatat tttcaaaagg
    21301 ctactgatat tcaaccagaa agtggctatg tatacatctc ttgggctcga tattacgaag
    21361 cgataaagga ttttagcact tcagaagagt acttatataa actttttgga gcgaaagata
    21421 tttcgtttca agataaattg cattatttag aaatactaac cagctattac agcaaaagaa
    21481 tggatggaat agaaaaaatg cataatttat atacttcttt attgcagtcg caccccatgg
    21541 agtatgtagc tcatactgca tatgctgaat ttttacttca acaaaaatta gtccctgagg
    21601 cgatagagca tttctataca gcagctttac tccatccttc gtgtctcgaa tgttgggtat
    21661 atttactaca atattataca gaaaaaaatg aaaatgaaaa agcctatcaa atagcgaaag
    21721 aggcgataga agccgtaccc aaccatcctg tgctattgtt ttattcaggc atgcttgctt
    21781 tgatgaatga ccagaaagag gatgctctgg ttcagttact tgaaatgatt aaatttaccg
    21841 attataacga gcctaattat gctgaaatga atgcagcggc acatgcgatg ttagtcgatt
    21901 tgtatctgat atataaaaaa gattacccaa aagctttaga gcagtgcgac cttgctctaa
    21961 aattaaaccc atcaaatcat gtggtaatga ataattgtgc ctacacactc gcattgcaaa
    22021 atgagcaact tgaaaaagcc gagaaattaa gtgctcaatc tttagaggca gag
//
LOCUS       DAOSRP010000013        21441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2653_length_21441_cov_4.858552, whole genome shotgun sequence.
ACCESSION   DAOSRP010000013 DAOSRP010000000
VERSION     DAOSRP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21441
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_21441_cov_4.858552"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLJ82_01660"
     CDS             complement(<1..237)
                     /locus_tag="PLJ82_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40249.1"
                     /translation="MIAQGNNTTIRIDYESPKEYEIADISVSGHHHYDTQTLIGLSGL
                     AVGDKITLPGDATANVIKRFWKQGLFSSVRLEITS"
     gene            complement(319..1059)
                     /locus_tag="PLJ82_01665"
     CDS             complement(319..1059)
                     /locus_tag="PLJ82_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOR40250.1"
                     /translation="MNSYLNNIDTTKVPTHVAIIMDGNGRWAKQIGKNRLFGHQNAIT
                     AIRQSTEFAAEIGIKFLTLYAFSTENWCRPDDEVKGLMELLVATIEAETPTLNKNNVR
                     LLSIGDTSRLPESARLRLNHCIETTSKNTGLSLVLALSYSSRWEILDATKHIAEKVKQ
                     NTLSVNDITEELFSSYLTTRTIPDPDLLIRTSGEFRISNFLLWQLSYAELYFTPILWP
                     DFTKEEFQKAIIDYQKRERRFGKTSEQV"
     gene            complement(1065..1763)
                     /locus_tag="PLJ82_01670"
     CDS             complement(1065..1763)
                     /locus_tag="PLJ82_01670"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOR40251.1"
                     /translation="MKKMKGIKKCVLFGVFVSLYSIANAQYNHEIGITGGGSFYLGDA
                     NLSLPFHKTNVAYGGFYVYNIDTRYALKAHVIAATIEGDSRDFNYIFPNQQFGYFDRK
                     LIDIGLNLQFNFFDLGQSKYYNSHNSITPYFMIGMGYLAYNNIYIGDNVYKFTIPMGI
                     GGKWKMNDRFTLGIEWKINKLFIDDLDVNNSESKILSNPYQTKNTSFFDTDWYSLASI
                     SLSYNLFNTRRFCR"
     gene            complement(1961..2851)
                     /gene="gldN"
                     /locus_tag="PLJ82_01675"
     CDS             complement(1961..2851)
                     /gene="gldN"
                     /locus_tag="PLJ82_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOR40252.1"
                     /translation="MKKTFYQLFFAGLFLMFGNIAVAQEEEGGVSFFGENGELATNIP
                     TSVSLEERKPIEYNNPRADDIIWQKVVYRIVDLREKMNFPLYFPEETSDNRQSLFTTI
                     FRLFERDQIKCYSYKDNVEFFTENELVKFDDIFSKYGMVRPVKRDSVSGDTTIVEFDE
                     SDIPNREILKYYVKEVWYFDKHNSHFNVRIMAICPIWYRQDYDLGLQKYPLFWVPFDL
                     LRPYLAQNEILVSDRNNGARETMDDLFIKRRFGSYIYKESSTRNRNLLQYNSTAEQMN
                     REQERIKNEIFNFEQDLWEY"
     gene            complement(2864..4417)
                     /gene="gldM"
                     /locus_tag="PLJ82_01680"
     CDS             complement(2864..4417)
                     /gene="gldM"
                     /locus_tag="PLJ82_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOR40253.1"
                     /translation="MGATNCPETPRQKMIGMMYLVLTAMLALNVSADVLNGFTLVHAS
                     LNESKSTTEMQNTILYGQFEDLKSQNPGRVGEWLEKANAVKAKTAEMYQMIEDLKVDI
                     VKQADGADGDVNNIKSKSNLDAGGQVALVTGRAKKMKEAIELYKDEMLKYVGKDSAKA
                     NYLNRTYNTGMITKENGDKISWEGAIFESMPVVASITLLTKVQADLKHTELEVVNYLK
                     NQIDAGDFRVNKIEGLVISESNYVTRGGRYSAKIVLAAQDSTKKPTITVNGRELDGNE
                     YIVQAGSVGTFPVKGTIRLPRPDGSTAEYDFESSYIVGEPTAIISADMMNVLYAGIQN
                     PISVSVPGIPAQNINVSVNNGALTRTAKGWNINPRVGQDCNISVSAKMADGRVQTIGS
                     KPFRVKPLPPPIAYIEFRDDKGNLQQYKGSRPFPKAQLVGSNGIRAALDDADLDVRYQ
                     VLGFDVNFFDSMGNTIVETSNNNSFSDKQMATMRQMPRGRKFFISRVRAKGPDGIERI
                     LPPIEVIVN"
     gene            complement(4464..5282)
                     /gene="gldL"
                     /locus_tag="PLJ82_01685"
     CDS             complement(4464..5282)
                     /gene="gldL"
                     /locus_tag="PLJ82_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOR40254.1"
                     /translation="MSKGSFLSSPQGKKMTAKAYGFGAAIVIIGALFKIMHFPGAGIM
                     LSVGMGVEALLFALSAFEDPHKEYDWSIVYPELDGGEAEERAPKVKAGAPLSVSGVNP
                     LPQDDVEKLKEGIRKISETASQFADISQASAASSNYVKNMNVASEAISAFASTQVGLK
                     NSSDSLFQSYRTVAESMTAAASDAKGYMQEMQGITRNLSSINASYELQLKNIGSQAQM
                     LEGMTTDMGKIKTAMNDAANETEAFKAQATKLTQQISSLNNVYGNMLNAFNARV"
     gene            complement(5328..6740)
                     /locus_tag="PLJ82_01690"
     CDS             complement(5328..6740)
                     /locus_tag="PLJ82_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR40255.1"
                     /translation="MKKLLILFAALAALVGCSNKGSGELVGVQGDAWKDPTPYGMVFI
                     PSGSFTMGPNDQDANWALSSQSKVVSVDAFWMDETEITNREYKQFVYWVRDSIARTKL
                     GEVDEAFLVTQDKRGNELEKPRINWKPKIPWSKANEEQAEAIDAMFYADDELIALTKE
                     LNGHILHYKYAWIDYNQAALKRNKFNTNTGKFEADLSLLGKGKDSADVMVKKDTAYLT
                     EEGYIVNQTKFVELKNRRDFISSKIINVYPDTLSWVRDFTYAYNEPYMKMYFYHDGYG
                     DYPVVGINWEQANAFCHWRTNFYNKAQIDNKRHIVQDYRLPTESEWEYAARGGRDLSM
                     YPWGGNYIRTAKGCYMANFKPQRGNYTVDGHMVAAKVASFPPNNYGLYDMSGNVAEWT
                     SSAFHESNYSFVNDLNPSYQYNAKASDPEIMKRKVIRGGSWKDIGFYLQCGTRTYEYQ
                     TESRSYIGFRCVRSVIGANY"
     gene            complement(6761..7705)
                     /locus_tag="PLJ82_01695"
     CDS             complement(6761..7705)
                     /locus_tag="PLJ82_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOR40256.1"
                     /translation="MKKKLLVWLFFVATTINGIAQQNMQFSLYTLNLNAINPASTGIN
                     EMIHISGVHRQQWLNIKRAPQITYMNIELPFSIENSKHGAGIGFLNQGIGLFTNQSIV
                     LKYAYHKKINEKTNLSGGLNIQFNSIGFNIKDATTGTSDFHSPNDPAIPTGSEISDLK
                     TDIGVGAMVKNETYLLGVALQNIVSPTYQLSNTEYTTPMNINLVGIYNIKTISSLYLI
                     QPSVLVKTDFATWQCELGSTIQYKEKYWGGLGYRFQDAIIFQVGMQLANGLTIGYAFD
                     LPISSMIYTSVGSHELSLSYSFKIEFSKKQTYKSIRHL"
     gene            complement(7819..8040)
                     /locus_tag="PLJ82_01700"
     CDS             complement(7819..8040)
                     /locus_tag="PLJ82_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOR40257.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLQEME
                     DEGEIYETIEDIWPDYPSKEDFFFNEDEY"
     gene            complement(8141..8656)
                     /locus_tag="PLJ82_01705"
     CDS             complement(8141..8656)
                     /locus_tag="PLJ82_01705"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOR40258.1"
                     /translation="MKIHTKTGDKGSTGLLGGTRVEKNDPRLEIYGTFDELTSFLGIL
                     KNQDIEDIHKKEIEYIQQNMIQVNAIFAIDYDNHSELALKYTFDTSAITYLEEKIDTY
                     TQLMPPVKGFVIPGETLSSAYCDACRTICRRAERRIYDITLHPHQQNAAIFINRLSDF
                     LYVLARKLTKL"
     gene            complement(8679..9464)
                     /locus_tag="PLJ82_01710"
     CDS             complement(8679..9464)
                     /locus_tag="PLJ82_01710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR40259.1"
                     /translation="MWQQKIWHIFQYFRYKIYAKHRKGHGVHSPFVFAFITDILEEKQ
                     PYYCFEEIEKTRQLLCQNNQKIFVDDFGTGRSNHRKISSIARKSLKSKKFAQLIFRII
                     AFYKPTTIIELGTSLGITSSYIASANKQAKVFSFEGSTEIAKIAKNNMDTLHLSNISI
                     ITGDITTQLPLFLANSAQIDMIFFDANHKKEPTLSYFNMCMEKIGKKALFIFDDIYWS
                     KEMKEAWEEICADTRIQVSIDLFSIGIVFIDPELKKKHYTIYY"
     gene            complement(9500..10717)
                     /gene="hflX"
                     /locus_tag="PLJ82_01715"
     CDS             complement(9500..10717)
                     /gene="hflX"
                     /locus_tag="PLJ82_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOR40260.1"
                     /translation="MISHSEIHIERVVLVGLITQQQNETKSKEYLKELAFLAETAGAI
                     VIKEFYQRVELPNIKTYVGKGKLLEIKNFIQEQEINLVIFDDELSPSQLRNIENTLEA
                     KALDRTSLILDIFAKRAQTAHAKTQVELAQYEYFLPRLTRLWTHLERQKGGIGLRGPG
                     ETQIETDRRIILDKIARLKAELIHIDKQKTIQRKNRGKMVRVALVGYTNVGKSTLMNL
                     LSKSNVFAENKLFATLDTTVRKITINNLAFLLSDTVGFIRKLPHGLVESFKSTLDEVR
                     EADILVHIVDISHPAFEEQIEVVEKTLHEIDNKEKLTILVFNKIDAFTYEKKDEDDLT
                     PLRKENISLQELQETWMSKLKQNCIFISAKEKTNIETLKDLLYERAKEIHTTRFPYND
                     FLYQQYDDDTSEE"
     gene            complement(10774..11460)
                     /locus_tag="PLJ82_01720"
     CDS             complement(10774..11460)
                     /locus_tag="PLJ82_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOR40261.1"
                     /translation="MEIKKTPKANLENKKTMAILIGLVLALGIMFIAFEWSKNEIKVY
                     EDAIQGEFVEDEEMIEVTFRDETPPPPAPPIQETVLSDIIDIRENKEDIQTKDFNSED
                     DKNKKVVIQAPIAAPVEDPEESRIHVVVERMPEFPGGEAAMNQFINRTIRYPVIAQEN
                     GIQGRVVVQFVVNTDGKIVDVEVVRGVEESLDKEAVRVVKAMPPWNPGRQGGKNVRVK
                     YTLPIRFRIG"
     gene            11638..12642
                     /gene="porQ"
                     /locus_tag="PLJ82_01725"
     CDS             11638..12642
                     /gene="porQ"
                     /locus_tag="PLJ82_01725"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOR40262.1"
                     /translation="MKVRINIFMSVVLLFSTYIQAQSLFTMSFLEQPVSAHHAALGGN
                     NTSYLGHDISFLVSNPALLSIQSSNELSVNVASYFAGSGYGSGIYSRLLHERGMLGAG
                     FQYMGYGEFTGFDEYGVETGTFSARDVAFFVSYSQQLNNYFSIGATVKPVLGVYERYV
                     SYALAGDMGIVFMDKENGLQVGFSAKNIGVRFAGYESDESASMLPLNLSLGASKKLLH
                     APFVFHLTMQQLQRWTYDVSTNVDSTSISFVGELARHFIVGVDVLPPSDKFWLALSYN
                     FLRGQELPVPEIFSLSGFSGGIGFFIKDVELGIGFGSYHQAATTLHISLRTDLTKFGL
                     "
     gene            12657..13355
                     /gene="cmk"
                     /locus_tag="PLJ82_01730"
     CDS             12657..13355
                     /gene="cmk"
                     /locus_tag="PLJ82_01730"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOR40263.1"
                     /translation="MTRKKIIIAIDGYSSCGKSTMAKELATSIDYIYVDTGAMYRAVT
                     WYCIENNIVQNDVLAVDKLVSLMPNICVEFYRTGDGSVHTKLNGKDVEKEIRGMLVSQ
                     YVSKVSALRFVREAMVTLQQKMGEQKGIVMDGRDIGTVVFPQAELKIFVTADARIRAK
                     RRYDELIAKGEVVCFDDILANIRERDFLDETREESPLRKAPDALVLDNGVLSMEEQNT
                     WLMNQYKRVLCEIY"
     gene            13340..14203
                     /locus_tag="PLJ82_01735"
     CDS             13340..14203
                     /locus_tag="PLJ82_01735"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR40264.1"
                     /translation="MRDILVEIDEGSGCCFGVVNATQKAENTLEKEGILFSLGDIVHN
                     SEEVSRLSNIGLKSIDNEQFEQLANTKVLIRAHGEPPSTYQKAKERGIQLVDATCPVV
                     LKLQKRIAEAYMNCKNNTQIVIYGKRGHAEVVGLVGQTVGEAIVIENCLQLDKLDFTK
                     NILLFSQTTQSVDGFKEIVAAIKEKIANDVNFDYKDTICRQVANRTPRIKAFALNHEV
                     VFFLSDKKSSNGKVLYEECKKINPRTYFLSKLEEFDKTVLEGVHSVGISGATSTPKWQ
                     MEKLKQLILLS"
     gene            14239..17037
                     /gene="uvrA"
                     /locus_tag="PLJ82_01740"
     CDS             14239..17037
                     /gene="uvrA"
                     /locus_tag="PLJ82_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR40265.1"
                     /translation="MKKKETIYIKGARVNNLKNIDVAIPRGKFVVITGVSGSGKSSLA
                     FDTLYAEGQRRYVESLSSYARQFLGRMSKPEVDAIIGIPPAIAVEQKVKTRNSRSTVG
                     TSTEIYDYLRMLFARIGKTFSPITGVEVKKHQTKDVLDFITSLPESSRVALLAPVIPT
                     TDETIEELVESLLKQGFSRMLYNGEFVPLGECCDQQLSTAMCELLIDRFPVTHDALSL
                     NRIADSVETAFYEGKGVCIVAEFDNNGKLTSKTEFSKIFAADGIVFDELTEQMFSFNN
                     PLGACPRCEGFGMIIGIDEDLVVPNKTLSVYDDAVACWKGEKMSEDKLNLIKHADKFD
                     FPVHKPYAALSPEQKKLLWTGNSYFYGINDFFTFLEQNQYKIQYRVMLSRYRGKTVCP
                     VCAGSRLRKEALYVKIHGKTIHDLVTMSITDLLLFFQQTSFTEYEKAVATRLLTEIMN
                     RLRYLQEVGLEYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPRN
                     TAMLVGVLKKLQALGNTVVVVEHDEHIMREADWLIDIGPDAGRLGGEVVLEGNPQSIL
                     VANTYTEEQKKRSHTLRYLLGEEAIPMPTTRRKWSNYLYIENANEHNLKNISVKFPLN
                     VLTVVTGVSGSGKSTLVRSILYASLRRYFGMAADRVGSFGKLSGSLHLVNAIEFVDQN
                     PIGKSSRSNPCTYLKAYDEIRKLFSEQQLAKQMGFTAAHFSFNVPGGRCEECQGEGTI
                     TIPMQFMADVVLECEACHGKRFKQDVLEVTYKGVSIADVLDMTVDQAIQFFAATPEST
                     TKKIVKRLQPLQDVGLGYVMLGQSSSTLSGGESQRVKLASFLSNETQEHTIFIFDEPT
                     TGLHFNDIKVLMQAFNTLIDKGHTVIIIEHNIDVIRVADHIIDLGKEGGDKGGYIVFE
                     GTPDEIKNCADSYTGVYL"
     gene            complement(17112..18956)
                     /gene="rpsA"
                     /locus_tag="PLJ82_01745"
     CDS             complement(17112..18956)
                     /gene="rpsA"
                     /locus_tag="PLJ82_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOR40266.1"
                     /translation="MTKEFIQPDESFDWGAYEQGATYTEISKEDLQKAYNQTLNQVNA
                     NEVVDGTVISMNKREVVVNIGYKSDGIVPISEFRYNPELKVGDKVEVFIESQEDKKGQ
                     LVLSHKKARANRSWDRVNEALEKEEIIKGFVKCRTKGGMIVDVFGIEAFLPGSQIDVK
                     PIRDYDVFVNKTMEFKVVKINQEFKNVVVSHKALIETELEAQKKEIIGKLEKGQVLEG
                     TVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPEEILQLDQKLNVVILDFDDDKKRI
                     ALGLKQLTPHPWAALDENLKVGDKVEGKIVVVADYGAFIEIAPGVEGLIHVSEMSWSQ
                     HLRTAQDFVKIGDKVEAVILTLDRDERKMSLGIKQLKEDPWLNIEAKYPVGSKHNAVV
                     RNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPSEFTQIGASIEIQVLEIDAENRRL
                     SLGHKQLEENPWDVFETIFTNDSVHEGTIIELFDKGAVISLQYGVEGFATPKHLVKED
                     GSQAKIDEKLAFKVIEFNKDAKRIILSHSRIFEDAQKAATVTEETVATPASTEEKPKR
                     TKKAKEEKVESAPAMEKTTLGDISGLAALKEEMDKKEKKEKKDKKDKE"
     gene            complement(19098..19937)
                     /locus_tag="PLJ82_01750"
     CDS             complement(19098..19937)
                     /locus_tag="PLJ82_01750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOR40267.1"
                     /translation="MKSLFLIKTILILALTQFSFMTNAQITNSHTLVKISTEYGDMTV
                     KLYNETPLHRDNFIKLVEQGFYNDLLFHRVINGFMIQGGDPQSKNAPKGKQLGSGDVG
                     YTIPAEFVYPQYYHKKGALAAARQGDQMNPKKASSGCQFYIVQGNKIGDAELQQTEQQ
                     AKHKAKQTLFYALADQHKENIKQLRINKDTAGLEKLQTELIAQVEKELATQSFTLPET
                     LKNDYKTLGGTPFLDNEYTVFGEVIDNLELIDKIAVSKTLSGDRPEKDIKMTLTILQK
                     GNY"
     gene            complement(19950..20132)
                     /locus_tag="PLJ82_01755"
     CDS             complement(19950..20132)
                     /locus_tag="PLJ82_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOR40268.1"
                     /translation="MDIQLIIVIIASILALLWVVWRVYRTFKQSDSAKNACGSCAHSA
                     SCKFKEECNDILHKQS"
     gene            complement(20137..21375)
                     /locus_tag="PLJ82_01760"
     CDS             complement(20137..21375)
                     /locus_tag="PLJ82_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40269.1"
                     /translation="MKKITTLCIALLVASITALAATKTTETFALAGKTRTYLQYIPSS
                     YNAETPSKVLILLHGLNGNMNFFENQGYNLATMADVTNTIILSLQALDEQDAEVTSLI
                     TLLGTSQATSFTNTGVWGAGVSIPVSSITGNLPLQYQQLFPSFFPTISSAGKAILNQH
                     IDDVAYINEVINKTKNTYTVNNEKVFMLGASMGGAMAYKYAFSANPQITAMAIISGFK
                     GAEVSTSGDFPVATCHFHSTNDEVVVYNGTAFNTPIQNLVTLLSGKNHTTSAEVIAIP
                     NAEEEDNMTVTAYDYQTASKPRFLFYKIEGANHMLTGLKDITIFTEALKFFNNEPILT
                     SITDTYVAQVNVSPNPTSDYIYVSVSGNYTLYTLQGNIILAGSAHENEAIAVGHLPKG
                     LYLLSIENNGTISSAKVAIK"
BASE COUNT         6109 a         3629 c         4108 g         7595 t
ORIGIN      
        1 agaggttatt tctagtcgta cagaagagaa tagaccttgt ttccaaaaac gtttaataac
       61 attggctgtg gcgtctcctg gtagtgtgat tttatctcct accgctagtc ctgagagacc
      121 aatcagcgtt tgtgtatcat aatggtgatg accagataca gagatgtctg ctatttcata
      181 ttctttagga ctttcataat caatacggat ggttgtattg ttaccttgtg caatcatcaa
      241 aagaggtaga aacaaaaagc atacaatgcc acacatttgt ttcgaaataa tatacaaata
      301 attttttttg ttcatgcttt acacttgttc acttgtttta ccaaacctac gttctctttt
      361 ttgataatca attattgctt tttgaaattc ttcttttgtg aaatctggcc acaaaatagg
      421 cgtaaaatac aattcggcat aggataattg ccatagtaag aaattactga tgcggaattc
      481 gccacttgtc ctaattaata aatcgggatc ggggattgtg cgagttgtta aatatgacga
      541 aaatagttct tcggtaatat catttaccga caatgtattt tgtttcactt tttcagcgat
      601 gtgttttgtt gcatctagaa tttcccatct tgatgagtaa cttaatgcca acactaatga
      661 taatccggta tttttagatg tagtttctat gcaatggttt aaacgtaatc gagcactttc
      721 tggcaagcga ctcgtgtctc caatacttag tagacgtaca ttgtttttgt ttagtgtggg
      781 tgtttctgct tcaatggttg caaccaaaag ttccattaag ccttttactt catcatctgg
      841 tctgcaccag ttttctgtag agaaagcata taaagtcaaa aatttaatcc caatctcagc
      901 ggcaaattcg gtgctttgcc gtatagctgt aatcgcgttt tgatgaccaa ataacctgtt
      961 tttccctatt tgtttagccc accttccatt gccatccata ataatggcta catgcgtagg
     1021 aactttggta gtatcaatgt tgtttaaata gctgttcata tctattatcg gcaaaatctg
     1081 cgtgtgttaa ataagttata cgataagcta atactagcaa gagaatacca atcagtgtca
     1141 aaaaatgatg tattttttgt ttgatatgga tttgataaaa tcttactttc actgttatta
     1201 acatctaaat catcaataaa taatttattg attttccatt ctatccccaa cgtaaatcgg
     1261 tcattcattt tccattttcc tccaataccc attggaatag taaatttata tacattatct
     1321 cctatataga tgttgttata cgccaaatat cccatgccta tcataaagta gggggtaatt
     1381 gaattatgac tattataata tttcgattga cctaaatcaa aaaaattaaa ttgcaaattt
     1441 aagccaatat caataagttt tctatcaaaa tatccaaact gttggtttgg aaagatgtag
     1501 ttaaaatccc tactatctcc ttctattgta gctgctataa cgtgagcttt caacgcatag
     1561 cgtgtgtcaa tattatagac ataaaaccct ccatacgcta cattcgtttt atgaaaaggg
     1621 agagaaagat tggcatctcc caaataaaaa gagcctcctc ctgtaatccc aatttcatga
     1681 ttatactgtg cgtttgctat cgaatataag gaaacgaata caccaaacag gacacatttt
     1741 tttatgcctt tcattttttt catacttttc acttagtacg tattcttata atcgatatag
     1801 tctctttttt tagttgatat tgagccaaca aatatactga atatcttctt attaatcggt
     1861 caaacactct cattattcca ttatatttaa caaaatataa gtaaaacaga aaagtcgctg
     1921 atgatgcagc gacttttctg tttttatgat ttacgtgtga ttagtattcc cacaaatctt
     1981 gttcaaaatt gaatatctcg tttttgatac gttcttgctc tcgattcatt tgttctgccg
     2041 tactattata ttgcaataag tttcgattac gtgtacttga ttctttatag atataactac
     2101 caaaccttcg tttgataaat aaatcatcca tcgtttcgcg agcaccatta tttctatctg
     2161 ataccaatat ttcattttga gccaaataag gtcttagcag atcaaaaggt acccaaaata
     2221 aaggatattt ctgcaatcct aaatcataat cttgacgata ccaaattgga catattgcca
     2281 tgatacgtac gttaaagtga gaattgtgtt tatcaaaata ccacacttct tttacgtaat
     2341 attttaaaat ctctctatta ggaatatcgc tttcatcaaa ctcaacaatt gttgtatcac
     2401 cagatactga atcccttttt acaggtctca ccattccata cttagaaaaa atgtcatcaa
     2461 atttgacaag ctcattttca gtaaaaaatt caacattgtc tttgtatgaa taacatttaa
     2521 tttgatctct ttcaaataat ctaaaaatgg tagtgaataa actttgacga ttatcacttg
     2581 tttcttcagg gaagtaaagt ggaaaattca tcttttctct aaggtcaaca atgcgataca
     2641 ccaccttttg ccaaataata tcatctgccc ttgggttgtt gtattcaatt ggttttcttt
     2701 cctccagtga tactgaggtt ggaatattgg tagcaagttc tccattttcg ccaaagaaag
     2761 aaactcctcc ttcttcttct tgtgctacag ctatattgcc aaacatcaag aataaccctg
     2821 cgaagaatag ttgataaaac gtttttttca tttcagtgaa attttaattt acaataactt
     2881 caattggagg caatatgcgt tcaataccat caggaccctt agcacgaaca cgtgaaataa
     2941 agaattttct acctctaggc atttgtctca tagttgccat ttgcttgtca gagaaagaat
     3001 tgttgttact tgtttctaca atagtattac ccattgaatc gaagaagtta acatcaaaac
     3061 ctaatacttg ataacgtaca tctaagtcag catcatctaa agctgcccta attccatttg
     3121 atcctaccaa ttgtgctttt ggaaaaggtc tactcccttt atattgttgt agatttcctt
     3181 tatcatctct aaattcgata tatgctattg gaggtggaag aggttttact ctaaacggtt
     3241 tagaaccgat tgtttgtaca cgtccatcag ccatttttgc agaaacagag atattgcaat
     3301 cttgacccac acgaggattg atattccatc cttttgctgt gcgagtaaga gcaccattgt
     3361 taacgcttac attgatgttt tgagcaggaa tacctggtac agaaacacta attggatttt
     3421 gaataccagc atatagtacg ttcatcatat ctgccgaaat aattgcagta ggttcgccca
     3481 ctatatacga actttcaaaa tcatattccg cagtacttcc atcaggacga ggtaaacgta
     3541 tagttccttt tactgggaaa gttccaacag aacccgcttg cacaatatat tcattgccat
     3601 ctaattctct tccatttaca gtaatggtag gttttttagt agagtcttgt gcagctaata
     3661 caattttagc tgagtaacga ccacctctag ttacataatt agattctgaa attacaagtc
     3721 cttcaatttt atttacacgg aagtctcctg catcaatttg gttttttagg taatttacta
     3781 cttctaattc agtgtgtttt aggtctgctt gcacttttgt gagtaaagtt atagaagcaa
     3841 ctacaggcat tgactcaaaa atggctcctt cccaagatat tttatctcca ttttctttag
     3901 taatcattcc tgtattataa gtgcgattta agtagtttgc ctttgccgaa tctttaccta
     3961 catatttaag catttcatct ttgtaaagtt caatcgcctc tttcattttt ttagctctac
     4021 ctgtaacaag agccacttga cctccagcat caaggttaga tttgctttta atgttattca
     4081 catcaccgtc agcaccatca gcttgtttta cgatatcaac ttttaagtct tcgatcatct
     4141 gatacatttc ggctgttttt gcttttactg catttgcttt ttcaagccat tcgccaacac
     4201 gtccaggatt ttgagatttt aaatcttcaa attgtccata aaggattgtg ttttgcattt
     4261 cagttgttga tttactttcg ttcaagcttg catgaaccaa ggtaaaccca ttcagtacgt
     4321 cagcagatac gttcaatgca agcattgccg taagtaccag gtacatcata cctatcattt
     4381 tctgtctcgg ggtttccgga caattagttg cacccatatt attttattat ttattttaaa
     4441 ttaggttaaa caattcttgt taattacact cttgcgttaa acgcattaag catattacca
     4501 tatacgttgt tcaagctgct aatttgttgt gttaatttgg tagcttgtgc tttaaatgct
     4561 tcagtttcgt tagcagcatc attcattgct gttttaattt tacccatatc agtagtcatt
     4621 ccttctaaca tttgagcttg tgatccaatg tttttcaatt gcagttcgta gctagcattg
     4681 atagaagaaa gattacgtgt gatgccttgc atttcttgca tataaccttt agcatcagaa
     4741 gctgctgcag tcatactttc tgctactgtt ctatatgatt ggaacaatga atcagaagag
     4801 tttttaagac ctacttgagt agaagcaaaa gcactgatag cttcagaagc aacgttcatg
     4861 tttttaacat aattagatga agctgcactt gcttgtgaaa tatctgcaaa ttgagaagct
     4921 gtttcactta ttttacggat accttctttt aatttttcta catcatcttg tggtaatgga
     4981 ttaactccag atacgcttaa tggtgcacca gcttttactt ttggagctct ttcttctgct
     5041 tctccaccat ctaattctgg ataaacgata ctccaatcat attctttgtg tggatcttca
     5101 aatgcagaca gtgcaaatag taatgcctca acacccatac cgactgataa cataatacca
     5161 gcaccgggga agtgcataat tttaaataaa gcaccgatga taactattgc agcaccaaag
     5221 ccatacgcct ttgccgtcat ttttttccct tgagggctac ttaagaaact tcctttagac
     5281 atttttgtgt gatttttagg ttaatagata atttattatt aatttattta gtaatttgct
     5341 ccgattacac ttcttacaca acgaaatcca atatacgaac gtgattctgt ttggtattca
     5401 taagttctag tgccacattg taaatagaat cctatgtctt tccatgagcc accacgaata
     5461 actttacgtt tcatgatttc aggatcgctt gctttggcat tgtattgata acttggattc
     5521 aagtcgttca caaaactata attagattcg tgaaacgcag acgaagtcca ttctgcaacg
     5581 ttgcctgaca tatcatacaa tccgtaatta ttaggaggga aagaagctac tttagcagcg
     5641 accatatgtc catcaactgt ataattacct cgttgaggtt taaagtttgc catatagcaa
     5701 ccttttgcag tacggatata atttccaccc catgggtaca tggataaatc gcgaccacca
     5761 cgagcagcgt attcccattc agactcagta gggaggcggt agtcttgtac aatatgacgt
     5821 ttgttgtcta tttgtgcttt attgtagaaa tttgttctcc aatggcagaa agcatttgct
     5881 tgctcccagt tgatgcctac aacaggatag tctccataac cgtcgtgata gaaatacatt
     5941 ttcatgtatg gctcgttgta tgcataggta aagtcgcgta cccaagaaag agtatctgga
     6001 taaacattaa ttattttaga agaaataaaa tctcttctgt tttttaattc aacaaatttt
     6061 gtttgattta cgatataacc ttcttctgta agataggcag tatctttttt taccattaca
     6121 tcagcactat cttttccttt acctagtaag gataaatctg cttcaaattt acctgtattt
     6181 gtgttaaact tgttgcgttt taaggctgct tgattatagt caatccatgc atatttgtaa
     6241 tggaggatat gtccattcaa ttcttttgta agagcaatta attcgtcatc agcataaaac
     6301 attgcatcta tagcttctgc ttgttcttca tttgcttttg accatggaat ttttggtttc
     6361 cagttaattc taggtttttc tagttcattt cctcttttgt cttgtgtaac aaggaaagct
     6421 tcatctactt caccaagttt agtacgtgcg attgagtcac gtacccaata cacaaattgt
     6481 ttgtactcac ggtttgtgat ttcggtttca tccatccaaa aagcatctac tgagaccact
     6541 ttagattgag aagaaagagc ccagttcgca tcttgatcgt tgggtcccat ggtaaaggat
     6601 ccagatggga taaacaccat accataaggg gtgggatctt tccaagcatc accttgtaca
     6661 ccaactaatt ctccacttcc tttgttactg caaccaacaa gggctgcaag agcggcaaat
     6721 aaaataagta attttttcat atctaatttt taggttaagc ttataaatgt cgaatgcttt
     6781 tgtatgtctg ttttttcgaa aattcaattt taaaactata tgataatgac aattcatgag
     6841 atccaacact agtatatatc atggatgaaa ttggtaaatc aaatgcatat cctattgtta
     6901 gtccatttgc caattgcata cctacttgaa aaataatagc atcttgaaat ctgtatccta
     6961 atccgcccca gtatttttct ttgtattgta tggttgaccc tagttcacat tgccatgttg
     7021 caaaatcagt tttcactaaa acagatggtt gtattaggta taacgaactt attgtcttta
     7081 tattgtatat accaactaaa tttatgttca ttggagtagt atactctgtg ttgctcaatt
     7141 gatatgttgg agatacaata ttctgtagtg ctacgccaag caagtatgtt tcatttttga
     7201 ccatggctcc aacgccgata tctgttttta aatcagatat ttcggaacct gttggaatgg
     7261 caggatcatt tggcgaatga aaatcacttg ttcctgtcgt tgcatctttt atattaaacc
     7321 caatactatt aaattgtata ttcaatcctc cactgaggtt tgttttttca tttatttttt
     7381 tgtgatatgc atactttaag acaatagact ggtttgtaaa aagaccaatc ccttgattta
     7441 aaaatccgat accagcacca tgtttggagt tttcaatcga aaagggtaac tcaatattca
     7501 tatacgtaat ttgaggtgct cttttgatgt tcaaccactg ttgacggtgt actccagata
     7561 tatgtatcat ctcgtttatt ccagtagatg ctggattaat ggcattcaaa tttagtgtgt
     7621 aaagactaaa ttgcatattt tgttgggcaa taccattgat agtggtagca acaaaaaaca
     7681 accatacgag aagttttttt ttcataaata atatggcggc taagctaaat cttaaccact
     7741 aggataaatt gccgcttgct atttattaac agattttttg caaatttatt gtgtaaaaca
     7801 acaaaaatgc agaaaaaatc aatattcatc ttcgttaaag aagaaatctt ctttacttgg
     7861 ataatcaggc caaatatctt ctattgtttc atatatttca ccttcatcct ccatctcttg
     7921 cagattttca attacctcta aaggagctcc agacctaatc gcgtaatcta tcaactcatc
     7981 tttggtggct ggccagggtg catcttctaa ttttgatgct aactctaacg tccaatacat
     8041 atcattcgat tattaagttt tattaaaaaa tccactaaaa ccgatcgcaa aggtaaaaat
     8101 atttttttag tttgcaatac tttttttaca attttttaaa ttacaatttt gttaattttc
     8161 gtgcgagtac atataaaaaa tcagacagtc tgttgataaa tatggctgca ttttgttgat
     8221 gggggtgtag agtaatgtca tatattcttc tttcggcacg tctacagatt gttctacatg
     8281 catcgcaata tgcagagcta agtgtttctc ctggtataac aaaccctttt accgggggca
     8341 ttaattgcgt gtatgtatca attttttctt ctaaataggt tattgcactg gtatcgaaag
     8401 tgtacttcag ggctaattca ctatgattgt cgtagtcaat agcaaatatg gcattaactt
     8461 gaatcatatt ttgttgaata tattcaattt cttttttgtg tatatcttct atatcttggt
     8521 tttttaagat acctagaaaa cttgtcaatt catcaaatgt tccgtaaatt tctaaacgtg
     8581 gatcgttctt ttctactcta gtgcctccta gcagcccagt gctacctttg tctccagtct
     8641 ttgtgtgtat tttcatgagt tgaaacgtat tttgtgattt aatagtaaat tgtatagtgt
     8701 tttttcttta gttcggggtc aataaaaacg ataccgatac taaataaatc gatggatact
     8761 tgaatacggg tgtctgcaca tatttcttcc catgcttctt tcatttcttt cgaccagtaa
     8821 atatcatcga atataaatag tgcttttttt ccaatttttt ccatacacat attgaaataa
     8881 gatagtgtag gttctttttt gtggttagcg tcaaaaaata tcatatcaat ttgagctgaa
     8941 ttggctaaga agagtggaag ttgagtagta atatctcctg taattatgct aatattggaa
     9001 aggtgaagtg tatccatgtt gtttttggct attttagcaa tttcggtaga tccttcaaat
     9061 gaaaaaacct tagcttgttt atttgcagaa gctatgtaag agcttgtaat gcctaggctt
     9121 gttcctaatt caataatagt agtaggtttg taaaatgcaa tgattcgaaa aattaactga
     9181 gcaaattttt tgcttttgag gcttttgcga gcgatagaac tgatttttct atgatttgag
     9241 cgtccggttc caaaatcatc cacaaatatt ttttgattgt tttggcacaa aagttggcgt
     9301 gttttttcta tttcttcaaa acaataatag ggctgttttt cttctaagat atcggtaata
     9361 aatgcaaaaa caaaggggga atgaacgcca tgtcctttac gatgttttgc atatatttta
     9421 tagcgaaaat attgaaagat atgccatatt ttttgttgcc acatagttcg tattgggtct
     9481 taggtttaat tatacttgtt tattcctctg acgtatcatc atcgtattgt tggtacaaaa
     9541 agtcgttgta tgggaatcgg gtggtatgaa tttcttttgc tcgttcgtat agcaagtctt
     9601 ttaatgtttc tatatttgtt ttttcttttg cagaaataaa tatacagttt tgttttagtt
     9661 ttgacatcca cgtttcctgt agttcttgca gtgaaatatt ttcttttcgt aagggggtta
     9721 aatcatcttc atcttttttt tcgtacgtaa acgcatcaat tttgttaaag acaagaatgg
     9781 ttaatttttc tttgttatca atttcatgca gtgttttttc tactacttca atttgttctt
     9841 caaatgcggg atgagaaata tctacgatgt gtactagtat atcggcttct cttacttcat
     9901 ctagagttga cttgaatgat tcgactaagc catgaggcaa ttttctgata aaacctactg
     9961 tgtcggatag tagaaatgcg agattgttaa tcgtaatttt tctaacggta gtgtcgagag
    10021 tggcgaataa tttattttcg gcaaatacat ttgatttact gagcaagttc attaaagttg
    10081 atttgcctac atttgtgtaa ccaactaatg ctactctaac cattttgcca cgatttttgc
    10141 gttgaatggt tttttgttta tctatatgga ttaattctgc tttaagtcta gctattttgt
    10201 ctaatattat acgtctgtct gtttcaattt gagtttcacc cggacctctt aatccgatac
    10261 ctcctttttg gcgttcgagg tgagtccata aacgtgttaa tcggggtaag aaatattcgt
    10321 attgagcgag ttcaacttgt gtttttgcgt gagctgtttg tgctcttttt gcaaaaatat
    10381 ctaaaatcag acttgttctg tctagggctt tagcttctag tgtgttttcg atattgcgga
    10441 gttgagaagg ggataattca tcatcaaaaa taacgaggtt gatttcttgt tcttgtataa
    10501 agttttttat ttcgagtagt tttccttttc ccacataggt ttttatattt gggagctcta
    10561 ctcgttggta aaactctttt atgacaatag ctccagcagt ttctgccaaa aacgcgagtt
    10621 ctttgagata ctcttttgat tttgtttcat tttgttgttg ggttattaga ccaactaata
    10681 ctacgcgttc aatatgtatt tcagaatgag agatcatagc ttatataggt taaaaaagct
    10741 caccaaatcg gtaaggcttt gtttttgtac tatctatcct attcggaacc gaattggtaa
    10801 tgtgtatttt acacgcacat ttttacctcc ttgtcgtcct ggattccatg gtggcattgc
    10861 ttttactaca cgaacagctt ctttgtctaa actttcttct actccgcgta caacttctac
    10921 gtccacaatt ttaccgtctg tatttaccac aaattgcact actacacgcc cttgtattcc
    10981 gttttcttga gcaataacag ggtagcgaat ggttcggttg atgaattgat tcatagcagc
    11041 ctctccacca gggaactctg gcatacgctc tactactacg tgtattctac tttcttcagg
    11101 atcttctact ggtgctgcaa taggagcttg aattactact tttttgtttt tatcatcttc
    11161 agagttgaaa tcttttgttt ggatatcttc tttattctca cgaatatcaa tgatatcact
    11221 taaaacggtt tcttggatag gtggcgctgg tggtggcggg gtttcatcac ggaaagttac
    11281 ctcaatcatc tcttcgtctt ctacaaattc tccttgaatt gcatcttcat acactttgat
    11341 ttcatttttt gaccattcga atgcaatgaa cataattcca agggctagaa ctaaaccaat
    11401 cagaattgcc attgtttttt tgttttccaa attggctttt ggggtctttt ttatttccat
    11461 atacgttgaa gtttgtcgtt tacacagttg cttgcaaatg tacaatattt ttttttaata
    11521 gcttgtatat cctgtaattt tttactgata ttttttttga acttgatttt gttgcttagt
    11581 aacatcgtta tttaccacaa aagatgtata tttgaatgtt ttttttagta taacgaaatg
    11641 aaggtaagaa ttaatatatt tatgtcggtt gttttactgt ttagtaccta catacaggca
    11701 caatcgcttt ttactatgag ttttttagag caaccggttt ctgctcatca tgctgcttta
    11761 ggagggaata atacatctta tttggggcat gatatttctt ttttagtatc caatccggcg
    11821 ttattgtcta ttcaatcatc caacgagttg tctgtgaatg tggcaagtta ttttgctggg
    11881 tcaggttatg gttcaggaat atatagtcgt ttgttacatg agcgaggcat gttaggggcg
    11941 ggatttcagt atatggggta tggagagttt actggatttg atgaatatgg tgtagaaacg
    12001 gggacgtttt cggcacgaga tgttgcgttc tttgtgagtt attcacaaca gttgaataat
    12061 tattttagta ttggagctac ggttaagcct gtgttggggg tttacgaacg ctatgtgtca
    12121 tatgcattgg ctggggatat ggggatagtt tttatggaca aggaaaatgg cttgcaggtt
    12181 gggttctctg ccaaaaatat tggagtaaga tttgctgggt atgaatctga tgaatcagct
    12241 tctatgttgc ccttaaattt gagtcttgga gcctcaaaaa agctattgca tgctcctttt
    12301 gtgtttcatc taacaatgca acagttacaa cgttggacgt atgatgtgtc tacaaatgtt
    12361 gattctacat ccatatcttt tgtgggggag ttggctcgcc attttattgt gggggtagat
    12421 gtattgcctc cttccgataa gttttggtta gcattatcgt ataatttttt acgcggacag
    12481 gaacttcctg ttcctgaaat attttctttg agtggttttt ctggcggtat tggttttttt
    12541 ataaaggatg ttgagttagg cattggtttt gggtcatacc atcaggctgc aactacttta
    12601 catatctcat tgcgtacaga tttaacgaaa tttggattgt agaaaatgcg ttttgtatga
    12661 ctaggaaaaa gattatcata gccatagatg gatattcatc atgtggtaaa agtacgatgg
    12721 caaaggaatt agctacctct atagattata tatatgtaga tactggagct atgtataggg
    12781 ctgtaacatg gtattgtatt gagaataata tagtacaaaa tgatgtgctt gcggtagata
    12841 agctcgtttc tcttatgcca aacatatgtg ttgagtttta tagaacgggt gatggctctg
    12901 ttcatacaaa gctaaatggg aaggatgtag agaaagaaat tagagggatg cttgtgtctc
    12961 agtatgtaag caaggtaagt gccttacgat ttgtgcgaga agctatggta actttacagc
    13021 aaaagatggg agaacaaaag gggattgtga tggacggacg tgatattgga actgttgttt
    13081 ttccgcaggc tgaattaaaa atatttgtaa cagcagatgc acgtattcgt gctaaaagac
    13141 gatatgatga attgatagcg aagggagagg ttgtttgttt tgatgatatt ttagcaaata
    13201 tacgagagcg ggatttttta gatgaaactc gagaagagag tcccttgcgt aaagcccctg
    13261 atgcattggt cttagataat ggggttttga gtatggaaga gcaaaatact tggttgatga
    13321 atcaatataa aagggtacta tgcgagatat attagtggaa attgacgaag ggtcaggatg
    13381 ttgttttgga gtggttaatg ctacacaaaa agctgaaaac acattagaaa aggaaggtat
    13441 attattttca ctaggagata ttgttcataa tagtgaggag gttagtcgtt tgtctaatat
    13501 agggttaaag tcgattgata atgagcaatt tgaacagcta gctaatacca aggtattgat
    13561 acgtgcacat ggggagcctc ctagtacata tcaaaaagcg aaagaaagag gcatacaatt
    13621 agtagacgcc acatgtcctg tggtattaaa attgcagaag cgaattgcag aagcgtatat
    13681 gaattgtaaa aacaatacac aaatagtgat atatgggaag agaggtcatg cagaggttgt
    13741 tgggttagtt ggacaaacag ttggagaagc tattgttatt gagaattgct tgcagttaga
    13801 taagttagat tttacaaaga atattctctt gttttctcaa acgactcaat ctgtagatgg
    13861 gttcaaggag attgtagctg ccataaagga gaaaatagct aacgatgtta attttgatta
    13921 taaagacacc atatgtagac aagtagcaaa taggactcca cgtataaaag catttgcctt
    13981 aaatcatgaa gtggtgtttt ttttgagtga taaaaagagt tctaacggaa aagtattgta
    14041 tgaggagtgt aagaagatta atccacgtac ttatttttta agtaaactcg aagaatttga
    14101 taaaacggta ctagaaggag tacattctgt aggaattagt ggggctactt ccacacccaa
    14161 gtggcagatg gagaagttga aacagttgat tttgctttca taaatcagct tttgggggat
    14221 tgaagcaatg gggataggat gaaaaaaaaa gaaacaatat atataaaagg ggctcgtgtc
    14281 aataatttaa aaaatattga tgtagcaata cctcgtggta agtttgtggt tataacaggt
    14341 gtttcgggtt caggtaagtc atcgttagca tttgatacct tatatgccga aggacaacgt
    14401 aggtatgtgg agagtttgtc atcgtatgca cgccaatttt taggacgcat gagtaaaccc
    14461 gaagtggatg ccataatagg tattcctcca gccattgctg tagaacagaa agtcaaaaca
    14521 cgcaattcgc gttccacagt tggcacatct accgaaattt acgactattt gcggatgttg
    14581 tttgctcgta taggcaaaac tttttcgcct atcactggag tagaagtcaa aaaacatcaa
    14641 accaaagatg tacttgattt tataacatcg ttgccagaaa gctcgcgtgt tgctttatta
    14701 gctcctgtga taccaacaac agatgaaacg atagaagagt tagttgaatc gttactgaaa
    14761 caaggtttta gtcgtatgtt atataatggc gagtttgtgc cattaggtga gtgttgtgat
    14821 caacaattat caacagcaat gtgtgagtta ttgattgacc gatttcctgt aacccatgat
    14881 gctctttctc ttaatcgaat tgccgactct gtagaaactg ccttttacga aggcaaagga
    14941 gtgtgtattg ttgcagagtt cgataacaat gggaagttga caagcaaaac tgaattttca
    15001 aaaatatttg ctgcggatgg tatcgtgttt gacgagctca cagagcaaat gtttagtttt
    15061 aacaatcctt taggagcgtg tcctcgatgc gaaggttttg gtatgattat tgggatagat
    15121 gaagatttgg ttgtgcccaa taagaccctt tcggtatacg atgatgctgt ggcatgttgg
    15181 aaaggagaaa agatgagtga agataaactt aacttgataa agcatgctga taaatttgat
    15241 tttcctgttc acaaaccgta tgctgcatta agccctgagc aaaaaaaact actttggact
    15301 gggaacagct atttttatgg aataaacgat ttttttacat ttttagaaca aaatcagtac
    15361 aagattcaat accgtgtaat gctgagtcgc tatagaggaa aaacggtatg ccctgtttgt
    15421 gcgggttcgc gattgagaaa agaagcttta tacgtgaaaa ttcatggcaa gacgatacac
    15481 gatttggtta ctatgtctat tactgatttg cttttattct ttcagcagac ttcatttact
    15541 gaatatgaaa aagccgtggc tacacgtttg cttacagaaa tcatgaatcg tttacgctat
    15601 ttacaagaag taggattaga atatttgact ttaaataggt tatctaacac cttgtcggga
    15661 ggtgaaagtc agcgaattaa tcttgctact tcgttgggga gtagtttggt tggctcgtta
    15721 tatattcttg atgagccaag cattggtctg catcccagaa ataccgctat gttggtaggt
    15781 gtactaaaaa aattacaggc attgggaaat acagtggtag tggtagaaca tgatgaacat
    15841 attatgcgtg aagccgattg gcttatagat ataggaccag atgcaggaag attaggagga
    15901 gaagtggtat tagaaggcaa tccacagtca atccttgtgg ctaatacata taccgaagaa
    15961 caaaaaaaac gctcgcacac attgcgttat ttactaggag aagaagcgat acctatgcca
    16021 actacacgca gaaagtggtc taattatttg tatatagaaa atgctaatga gcataatttg
    16081 aagaatattt cggtaaaatt tccgttgaat gtactgactg tagttactgg tgtaagtggc
    16141 tcgggaaaat ctactttagt acgttctata ttatatgcat cacttcggcg ttattttggt
    16201 atggcggctg accgtgtggg tagttttgga aaattatctg gaagtttgca tttggttaac
    16261 gccatagagt ttgttgacca aaatccgata gggaaatcat cgcgttctaa tccgtgtact
    16321 tatctgaaag cctatgatga aataagaaaa ttattttctg aacaacaatt agccaaacaa
    16381 atgggtttta ctgctgcaca tttttcgttt aatgtacctg gaggtagatg tgaagagtgt
    16441 caaggagaag gtacgataac gatacctatg cagtttatgg ctgatgtggt actagagtgt
    16501 gaggcgtgtc atggtaagcg gtttaagcaa gatgtgttgg aggtaacata caaaggagtt
    16561 agcattgctg atgtgttgga tatgacagtt gatcaagcca ttcaattttt tgcagcaacg
    16621 cctgaatcta caacaaagaa aatagtaaaa cgcttacaac cattacaaga tgtgggtttg
    16681 ggttatgtta tgttggggca atcgtcgagt accttgtcgg gaggggagag tcagcgtgtg
    16741 aaattagcct catttttgag caacgaaact caagagcata cgatttttat atttgacgaa
    16801 cctactactg ggttgcattt taatgatatt aaagtattga tgcaagcatt taatactctt
    16861 attgacaaag gacataccgt tattattatt gaacacaata tagatgtgat tagagttgcc
    16921 gatcatataa ttgatttggg taaagaggga ggagataagg gaggttatat tgtatttgaa
    16981 ggcactccag atgagataaa aaactgtgca gattcgtata caggagttta tttgtgatag
    17041 cacattgaaa aaaaaattaa aggcgatact tgatgaagag tatcgccttt aattatatag
    17101 tgaaagatag cttattcttt gtctttttta tctttctttt ctttcttttc ttttttgtcc
    17161 atttcttctt tcaaagcagc caaaccgcta atatcgccta aagtagtttt ttccattgct
    17221 ggagctgatt ctactttttc ttctttggct ttttttgttc tctttggttt ttcttctgtg
    17281 ctagctggag ttgctactgt ttcttctgtt acggtagctg ctttttgagc atcttcgaaa
    17341 atacggctat gagaaagaat gatacgttta gcatctttgt taaattcaat tactttgaat
    17401 gctagttttt cgtcaatttt agcttgcgaa ccatcttctt ttactaaatg tttaggagtt
    17461 gcaaaacctt ctacaccata ttgaagagag attactgccc ctttgtcgaa tagttctatg
    17521 atagtacctt cgtgaacaga gtcgtttgta aagatagttt cgaatacatc ccaaggattt
    17581 tcttctaatt gtttgtgacc aaggcttaag cgacggtttt cagcatcaat ttccaatact
    17641 tgaatttcga tagatgcacc aatttgagta aattctgatg ggtgtttgat ttttttagtc
    17701 cacgaaaggt cagaaatatg gattaaacca tctacgcctt cttcaatttc tacaaataca
    17761 ccaaagttag taaagttacg tactactgca ttgtgtttgc ttccgactgg gtatttagct
    17821 tcgatattta accatgggtc ttcttttagt tgtttgatac ccaatgacat ttttctttcg
    17881 tcgcgatcaa gggttaaaat aacagcttct actttgtcgc caattttaac gaaatcttga
    17941 gcagtgcgta agtgttgcga ccagctcatt tctgatacgt ggattaaacc ttctacgcca
    18001 ggagcaattt cgataaatgc accgtaatcg gctactacta caattttacc ttctacttta
    18061 tcgcctactt ttaagttttc gtcaagagca gcccatgggt gaggagtaag ttgttttaag
    18121 cctaaagcaa tacgtttttt gtcatcatca aaatcaagga ttacaacgtt caatttttgg
    18181 tctagttgta aaatttcttc tggatggttg atgcgacccc aagaaaggtc tgtgatatgg
    18241 ataagaccat ctacgccacc aaggtcgata aatacaccgt acgaagtaat gtttttcaca
    18301 gtaccttcta gtacttgacc tttttctagt ttgccgataa tttctttctt ttgagcttct
    18361 aattctgttt cgataagtgc tttgtgcgat accacaacgt ttttgaattc ttggttgatt
    18421 tttaccactt tgaattccat tgttttgttt acgaatacat cgtaatctct aattggtttc
    18481 acatctattt gagaacctgg taagaatgct tcgataccga atacgtctac aatcataccg
    18541 ccttttgtgc gacattttac gaatcctttg ataatttctt ctttttcaag ggcttcgttt
    18601 actctatccc aagagcggtt tgcacgtgct tttttgtgag aaagtactaa ttgtcctttt
    18661 ttgtcttctt gcgattcgat aaatacttct actttatcgc ctacttttag ttcaggattg
    18721 tagcgaaatt cgctaattgg cacgataccg tctgatttgt aaccgatgtt tactactact
    18781 tcgcgtttgt tcatcgaaat aacagtacca tccactactt cgttggcatt tacttggttg
    18841 agtgtttgat tgtaagcctt ttggaggtct tctttgctaa tttctgtgta ggtagcacct
    18901 tgttcgtatg ctccccagtc gaaactctca tcaggttgaa taaattcttt tgtcatgttt
    18961 tttgtttcct aattagttgt tagacattat gttgattaaa aatgagccgc aaaggtaatg
    19021 gtttattttg agataaccca cttttttgca aaaattttta gtgtaaaaat gctgcaaata
    19081 gttgactgct tgattcttta gtaattccct ttttgtagaa tggttagtgt cattttgatg
    19141 tccttttctg gtcggtcgcc tgatagggtt ttgctaactg ctattttatc aataagctct
    19201 aggttgtcga ttacttcgcc aaaaacggtg tattcattgt ccaaaaaggg tgtaccaccc
    19261 agcgttttgt agtcgttttt gagagtttcg ggcagggtga atgattgagt agcgagttct
    19321 ttttctactt gagcaattaa ttctgtttgt agtttttcta agccagcagt atctttgttt
    19381 atacgtagct gtttgatatt ttctttgtgt tggtcggcaa gggcataaaa cagtgtttgt
    19441 ttggctttgt gtttcgcttg ctgttctgtt tgttgtagtt cggcatcgcc tattttgttg
    19501 ccttgtacaa tataaaattg acaacccgat gaggcttttt taggattcat ttggtcgcct
    19561 tgacgagcgg ctgccaatgc tccttttttg tggtaatact gtggatacac aaattcggca
    19621 ggtatggtat aacccacatc gccactacca agctgtttgc ctttgggtgc gtttttcgat
    19681 tgagggtcgc ctccttgaat cataaatccg ttgataacgc gatgaaataa taggtcgttg
    19741 taaaaacctt gttcaactaa tttgataaaa ttatcgcggt gtaggggagt ttcgttatat
    19801 aattttacgg tcatatcgcc gtattcggta ctaattttta cgagggtgtg cgagttggtt
    19861 atttgtgcgt ttgtcataaa agagaattgg gtaagtgcga gtattaaaat ggttttgatt
    19921 aaaaaaagcg atttcataaa aatgaaaaac taagattgtt tgtgtagaat atcgttgcat
    19981 tcttctttga acttgcaact ggcactatgg gcacaactac cgcaggcatt ttttgctgaa
    20041 tcggactgtt taaacgtgcg atacaccctc catactaccc acaatagggc gagtatggag
    20101 gctataatga ctatgataag ttgtatgtcc atttacttat ttaatggcta ctttggcaga
    20161 gctgattgtg ccgttgtttt caatagatag tagatacaag cctttgggta aatgacccac
    20221 agcaatggct tcgttttcgt gagcactacc agcgagtatg atattgcctt gtaatgtgta
    20281 cagagtatag ttgccactaa cagatacata gatataatcg ctagtagggt taggagaaac
    20341 gtttacttga gctacgtatg tgtcggtaat agaagtaaga attggttcgt tgttgaaaaa
    20401 tttcaaagct tctgtaaaaa tggtaatatc ttttaagccc gttagcatgt ggtttgcacc
    20461 ttctattttg taaaacaaaa agcgaggttt gcttgctgtt tggtagtcgt aggcagttac
    20521 cgtcatgtta tcttcttctt cggcattggg tatggcaatt acttcggcag aagttgtgtg
    20581 gtttttgccc gacagtaagg taaccaagtt ttggataggt gtattaaatg cagttccgtt
    20641 gtataccact acttcgtcgt tggtgctatg aaaatggcag gtggcaacgg ggaaatcccc
    20701 tgatgtgctt acctcggcac ccttaaaacc gctaatgata gccatagccg taatttgcgg
    20761 attagcacta aaagcatatt tatatgccat agcaccaccc atagatgctc ctagcataaa
    20821 tactttttcg ttgtttacgg tgtaggtatt ttttgtttta ttgattacct cgttgatgta
    20881 ggctacatcg tctatgtgtt ggttgagtat tgcttttcct gcagatgaaa ttgtaggaaa
    20941 aaaactagga aaaagttgtt gatattgtaa tggtaaattt cctgtaatgc tacttacggg
    21001 aatgcttaca cccgcacccc atacgcccgt attggtaaac gaggtagctt gagaagttcc
    21061 gagaagtgtt attaaactag ttacttcagc atcttgctca tctaaggctt gcaacgataa
    21121 aataatggtg ttggtaacat ctgccatagt cgctaagttg tagccttggt tttcgaaaaa
    21181 attcatgttg ccattcagtc cgtgtaacag gatcaatacc ttgctaggtg tttcggcatt
    21241 gtaggaacta gggatgtatt gcaggtaggt gcgtgttttg cctgctaacg caaaggtttc
    21301 ggtggtttta gtagcagcca atgctgtaat tgatgctacc aatagggcaa tgcaaagtgt
    21361 agtgattttt ttcatacagt ttttttgttt tttttgtctt acacgcttat tgtatttgca
    21421 aacgcataag tagtttatac t
//
LOCUS       DAOSRP010000014        21412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2654_length_21412_cov_5.687316, whole genome shotgun sequence.
ACCESSION   DAOSRP010000014 DAOSRP010000000
VERSION     DAOSRP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21412
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2654_length_21412_cov_5.687316"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PLJ82_01765"
     CDS             <1..1352
                     /locus_tag="PLJ82_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR40270.1"
                     /translation="AEFKIIEPTTKVVLTTQNIPYGAKLYFQSGVTVEKGAVICEWDP
                     FNAVVISEVAGKMTFESMVENITFKTESDEQTGLKERIIIESKDKSKVPAAHILDEKG
                     EILRTYALPVGAHIAITEGSKIKIGQILLKMPRAVGKSGDITGGLPRVTELFEARNPS
                     NPAIVSEIDGEITFGKIKRGQREIVVTSKSGEVKKYLVPLSKQILVQENDFVRAGNPL
                     SDGAITPSDILAIKGPTAVQEYIVNEVQDVYRLQGVKINDKHFEVIVRQMMRKVEIED
                     PGDTRFLEKQTVDKQEFMEENDRIWGKKVVVNPGDSEVLMAGQIIAPRKLRDENSMLK
                     RKDLRTVEVRDAMSATSSAILQGITRASLQTTSFMSAASFQETTKVLNDAAINGKVDK
                     LEGLKENVICGHLIPAGTGQPELEKIVVASRDEYEKIMQAKRSVTDVNLKEAEEVSF"
     gene            complement(1509..2891)
                     /locus_tag="PLJ82_01770"
     CDS             complement(1509..2891)
                     /locus_tag="PLJ82_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOR40271.1"
                     /translation="MKKCIYLLLFIFLSIICVSCISGENQGNQEDIPDLEGILQRGKI
                     RVITLYGPLSYFNYKENVMGYEYEFAQQLSQELGVEMEVIVAKNIANMIELLENGEGD
                     LIAYRVPRTLEYQQRVSFTDREYVTSQVVIQLQSDSILTDVLQLAGKEVYVPESSIYH
                     DRIVDLNNEIGGGIHIVAVNDSVSVDALIVQTAQQLIPMTIADADIARLNKTYFSSID
                     YSLSISFPQRYAWAVRNDNPELLAYINKWNAKNAKNMKLSSIYNKYFQRTKYFESEGF
                     EQKKLHQLSAFDDYFKKYASIVSWDWRLIAAIAYKESKFDPTIVSWAGAAGIMQLMPS
                     TARTMGFSEHDLHSPEQSIKAGVKYLQHLQGIFQQIPVKEERIKFVLAAYNSGPGHLF
                     DARALALKYGKDPNIWHDNVEIYVRLKSNPEYYTDEVCKHGYLRGDETANYVEFVENK
                     FEEYKTRIGK"
     gene            complement(2913..3650)
                     /locus_tag="PLJ82_01775"
     CDS             complement(2913..3650)
                     /locus_tag="PLJ82_01775"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR40272.1"
                     /translation="MQQTTLEKILFLDIETVPQEATIKKMPESLAHLWMDKLDTLKRR
                     MPDRYDDDSSIDDLYRKEAGIYAEFGKIVCISVGIIYLKDGEKHCRIKSFFGHDEKTL
                     LTEFSGMLLKFMQSAEYQLCGHNIKEFDVPYIARRMLINGLPLPDVLQVAGKKPWEVK
                     FLDTLELWRFGDYKNYTSLKLLTAIFNIPTPKDDIDGSEVATVYYEHGDINRIAIYCQ
                     KDVVATIQVYLKLIGEPIIQDKHIQLV"
     gene            complement(3656..4264)
                     /gene="udk"
                     /locus_tag="PLJ82_01780"
     CDS             complement(3656..4264)
                     /gene="udk"
                     /locus_tag="PLJ82_01780"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOR40273.1"
                     /translation="MIIIGIAGGTGSGKSTVAKEILSQLPDGDIAFLPQDSYYKDSSH
                     LPMDKRIEINFDHPDSIEFDLLISHIKELKKGNSVQQPIYSYITCSRAEETVLIKPAK
                     IIVVEGILIFTDKELRKLMDLKVFVDADSDDRLIRVINRDIIERGRSVNSVIERYQKV
                     LKPMHLQFIEPTKRYADLIIPEGGHNKVAISILQHYIDNKLR"
     gene            4409..4855
                     /locus_tag="PLJ82_01785"
     CDS             4409..4855
                     /locus_tag="PLJ82_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR40274.1"
                     /translation="MSVNKVILVGNVGKDPEVRYLENNVAVAKFSLATTEKGYKMNNG
                     TVVPDRTEWHNIVMWRGLAEIAEKFVKKGSQLYIEGKIQSRTWDDKNGVKHYATEILC
                     ENMQMLSKKSEAESGNAPLNDKSVSTKAEETPFGNSDNIADDDLPF"
     gene            complement(4937..6139)
                     /locus_tag="PLJ82_01790"
     CDS             complement(4937..6139)
                     /locus_tag="PLJ82_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOR40275.1"
                     /translation="MHTTKQITNNIYYVGVNDRTKHLFESLWEIPAGVSYNSYLIVDE
                     KVALVDTVDACYSDLFLSKIEGILGEKPIDYLIINHMEPDHSASIATIKRKYPSIQIV
                     GNAKTFGMLDGFFNIKDNLYEIKEADTLTLGKTVLRFYLTPMVHWVETMMTFDETNKV
                     LFSGDAFGCFGALDGGVLDSQINVDKFWTEMERYYSNIVGKYGYPVQRALAKLSNLDI
                     QTICSTHGPVWQKYLQKTISIYNDLSLYQGKKGVVVMYGSMYGNTEQMAEVIADTLVA
                     NGIENVVMHNVSKSTPSYIIADIFRYQGLIIGSPTYSNELFPLVESMLSKIKNRDIKN
                     RYFAYFGSFSWAGVANKKLAAFAEQVGFEVVYEGGVEQKFALKHDKYVECVKLAESMA
                     QRLNNSSL"
     gene            6410..7540
                     /locus_tag="PLJ82_01795"
     CDS             6410..7540
                     /locus_tag="PLJ82_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR40276.1"
                     /translation="MQFSQIIGQDIIKQQLITSVREGRIPHAQLFLGQEGVGKLALAI
                     AYAQYVSCLNKGEHDSCGTCSSCIKYEKLIHPDLHMVFPFAKDGKTKEICDDLLPEWR
                     EFVTKNVYFSLEQWMDNIGAGNKQATIYANESNEILRKLQLTTYESAYKIMIIWLPEK
                     MNITCANKLLKILEEPPTKTLFILVSEQVEKIISTIISRTQMIKIPLIETHTMSSYLQ
                     AKHSISKENSINIARIANGNYIKALSIIKATEESQLNFTRYQEIMRYAYKNDVLATKK
                     WAEEMTSSSMGREKQKSFLDYAQHITRECFIKNIEINELNYLIDYEENFSSGFARFVN
                     EKNIQALMDFFADAATDIEQNVQGKFVFFDLGLKLMTQIKQN"
     gene            7553..8659
                     /gene="ricT"
                     /locus_tag="PLJ82_01800"
                     /pseudo
     CDS             7553..8659
                     /gene="ricT"
                     /locus_tag="PLJ82_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            8679..9167
                     /locus_tag="PLJ82_01805"
     CDS             8679..9167
                     /locus_tag="PLJ82_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOR40277.1"
                     /translation="MKQILHHSNNLLIALSVGLLFSLQSCGEKSVYNEYNKIATVGWD
                     FGTPARFETEINDTLSAYDITIQVRNNTDYAYQNFWIFVESTSPSGIVAKDTLEFLLA
                     DNTGKWLGKGISSTKEISGLYLENIKFPELGTYSFSLYQGMRSASLKGIESVGLRIVK
                     RQ"
     gene            9169..9912
                     /gene="trmB"
                     /locus_tag="PLJ82_01810"
     CDS             9169..9912
                     /gene="trmB"
                     /locus_tag="PLJ82_01810"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOR40278.1"
                     /translation="MGKNKLKKFEDMDSYPHVFQFPFSKIQEQGFHLKNHWHTNFFQN
                     NHPIVLELGCGKGEYTVELAKLFPEKNFIGIDIKGARMWTGAKASLEQKMNNVAFIRT
                     NIELITHFFGTNEVSEIWITFPDPQMKKASKRLTSTRFMALYNEILQPNGLIHLKTDS
                     PFLYTYTQLMIEKNALDIHFQTDDLYHSNWVNEILSIQTFYEKQWIERGLSIKYIQFK
                     INKNTPLLEPEEEIEHDSYRSFNRSKRSM"
     gene            9937..10998
                     /locus_tag="PLJ82_01815"
     CDS             9937..10998
                     /locus_tag="PLJ82_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOR40279.1"
                     /translation="MAIYPQLIIDALRKVRYPGNNKDIVTNEMLEDDIRIEGNKVSFS
                     LILKANDPFAKSLVKAAEQAILTYVGEEIDIKGNISIKALPAPNPMLNPRSLTHIKHK
                     IAISSAKGGVGKSTVSSNLAVALANKGYKVGLLDADIYGPSIPKMFDIEEERPTVEVI
                     DGKDWIVPVEKMGIKILSIAFFIEKDNAIIWRGAMASNALKQLINDTLWGDLDFFLID
                     LPPGTGDVHLTLLQTIDLNGAIIVSTPQQVALLDTVKGINMFLNEKINIPIVGLIENM
                     SWFTPAELPNNKYYIFGKEGMKNLAENMKLPFLGQIPIVQSICESGDSGSPIIINENS
                     IVGTSFDTITENILRNLKK"
     gene            11066..11422
                     /gene="rsfS"
                     /locus_tag="PLJ82_01820"
     CDS             11066..11422
                     /gene="rsfS"
                     /locus_tag="PLJ82_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR40280.1"
                     /translation="MTDTQQLVNSIIAGIQEKKGSKIVVADLSKLSNSICSYFIICEG
                     GSNTQVDAIASSLKDYVKKETKERPLGMEGLENCEWVVMDYGDVMVHIMQREPRKFYD
                     LENLWQDAKLTEIADI"
     gene            11459..13438
                     /gene="ftsH"
                     /locus_tag="PLJ82_01825"
     CDS             11459..13438
                     /gene="ftsH"
                     /locus_tag="PLJ82_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOR40281.1"
                     /translation="MENENKQGKQPNNNKPKGKFIVNMYWFYGIIAAVLIALNIFMNS
                     DASKEVSYSEFRNYAEKGYVESIVVITNKNAAEVVIKQDSSILNTVFGDDKGKVNSLI
                     VTIPSPEKMEEFVADNDKNLNIKTDIKYEERKNYLDTLLWSFGPFILIIIIWIFIMRR
                     MSGGGAGGNVFNVGKSKAQLFDKGANTMSVTFKDIAGLAEAKQEIQEIVDFLKNPSKY
                     TNLGGKIPKGALLVGPPGTGKTLLAKAVAGEAHVPFFSMSGSDFVEMFVGVGASRVRD
                     LFRQAKEKAPCIIFIDEIDAIGRARGKNPSFNSNDERENTLNQLLTEMDGFGSNSGVI
                     ILAATNRAEILDKALMRAGRFDRQIHVDLPDVHERNEIFGVHLRPLKLSPELNIEFLA
                     KQTPGFSGADIANVCNEAALIAARKNKEVVEKQDFLDAVDRIIGGMEKKNKITTTAEK
                     ASIATHEAGHAAVSWLLEYANPLVKVTIVPRGKALGAAWYLPEERQLTMKEQILDEMC
                     AILGGRAAEDVVFNRISTGALNDLERATKMAYSIVVYYGMSNKLSNVSYFDSTGGEYS
                     FTKPYSEKTAEIIDTEVQSIIAEQYERAKKIISNNKENHAQLSQLLIEKEVIFAEDME
                     QIFGKRPWASRMDELISESEKNKINTSTTEATTTL"
     gene            13435..14241
                     /locus_tag="PLJ82_01830"
     CDS             13435..14241
                     /locus_tag="PLJ82_01830"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOR40282.1"
                     /translation="MIKNFIQRTLTGFMFVASILLSIYFQATYYTFSILFFGFTAIAL
                     FEFYRIINQYKEVTLITGLPIATGLILYISTFLLAAGIAHYSILVFYAVGIVGVIIAE
                     LYRKQANPFHNIAFIVLGQFFIAFPFSILNAIAFAEIYWLFALFMLIWVYDSCAYMVG
                     VMFGKHRMFERISPKKSWEGFIGGFVLTLAATLIFAYFTPDIAIWKWLLFGVLIVIFG
                     TWGDLTESLLKRTVDMKDSGNILPGHGGILDRFDSLLLLAPIIYFFLQLI"
     gene            14251..14919
                     /locus_tag="PLJ82_01835"
     CDS             14251..14919
                     /locus_tag="PLJ82_01835"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOR40283.1"
                     /translation="MIKIHKEGRMLLLVLFLALTIVNTLLFFIFDNAIVPTIALVVSI
                     GFFIFFTHFFRSPERIVLADEHYVVAPADGKIVVIEPVEEHEFLKEKRLQVSIFMSPF
                     DVHANWYPIAGKVIHVAHHDGRKMAAYLPKSSTENERSTVVIETQHKQLVLMRQIAGA
                     LARRIVTYAKVGDNCKINQQFGFIKFGSRVDLYLPLDSEILVDLNEEVVGNETIIAKL
                     KHEV"
     gene            14972..16510
                     /locus_tag="PLJ82_01840"
     CDS             14972..16510
                     /locus_tag="PLJ82_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOR40284.1"
                     /translation="MLVKTFGAAVHGIDASIITIEVNVSQGIKFFLVGLPDNAVKESH
                     ERIVSALQYNGHKFPRQQIIINMAPADIRKEGSAYDLPLAMGIMAAAEQIACEKINEY
                     LLMGELSLDGNIMPIKGALPIAIKAREAGFKGLILPKQNAKEAAVVNNLEVLGVENIQ
                     EVIGFLNSETTIEPTIVHTRDEFYSMASDFEMDFSDVKGQETVKRAMEVAAAGGHNII
                     MIGPPGAGKSMLAKRIPSILPPLTLHEALETTKIHSVAGKIEKGISLMYKRPFRSPHH
                     TISDVALVGGGSFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRKICISRSK
                     FSIEYPASFMLVSSMNPCPCGYYNHPEKDCVCGPGIVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSCTKPTEHSEVIRERVIKARKIQEERFKENDGVHCNAQMSSKLLREHTKL
                     NDTSQQLLKVAMQRLNLSARAYDRILKVARTIADLDDAKDIQVEHISEAINYRNLDRE
                     GWAG"
     gene            16538..17527
                     /gene="trpS"
                     /locus_tag="PLJ82_01845"
     CDS             16538..17527
                     /gene="trpS"
                     /locus_tag="PLJ82_01845"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOR40285.1"
                     /translation="METVLSGIRSTGNLHLGNYYGALRNFIKMQEEYQCFFFIADYHS
                     LTTHPNPHEMHGNVRNVLTEYLAAGIDPDKATIYVQSDVPEIPELYLLLNMHAYLGEL
                     SKTASFKEKARKQPENVNAGLLTYPTLMAADILIHNAHKVPVGKDQEQHLEMTRKFAR
                     RFNNFYQVEYFREPAAFNFGEDLLKIPGLDGSGKMGKSEGNCVFLADDAKTIEKKVKR
                     ALTDAGPTTPNSTKPDYIENLFTILKVVSTPEVVTHFEEKWNTCDIRYGDLKKQLAED
                     IIRATSPIRERIIDIQNNEAYLAKVTKEGAEKARISAAKTLREVREIMGFKKF"
     gene            17529..18461
                     /locus_tag="PLJ82_01850"
     CDS             17529..18461
                     /locus_tag="PLJ82_01850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOR40286.1"
                     /translation="MPKVVGIGETVYDIIFENNRPIDGKPGGSVFNALISLGRLQQQT
                     AFISEVGDDTVGKIILDFLAQNKVNADYVAQFDGTKTALSLAFLDENKNANYTFYKDY
                     SAQKAPHKTPPITSKDIVLFGSFFALNSTVRQQVTTLLDTANKESAMVYYDVNFRKTH
                     ISKRHELLPALIENMKYADIVKGSEEDFDHLYDEKDPITIYQKYIAPHCSTFIYTQGA
                     NGAMVFTPTYSLHVPSIPIVPISTIGAGDTFNAGIIFALLQQNIAKDTLCNVQQHEWE
                     AILQQAIDCATHVCMLYDNYITVDFANKKLDLFR"
     gene            complement(18450..18767)
                     /locus_tag="PLJ82_01855"
     CDS             complement(18450..18767)
                     /locus_tag="PLJ82_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOR40287.1"
                     /translation="MDLHTIDGVRLHAWNSFPSGHTMAAFAFFLSLALIVKNQLLKFF
                     FFAMSLLVGFSRIYLNQHFLEDTLAGSLLAVVIALTMYPFFNTQQTWGNKGLMSLANK
                     STK"
     gene            complement(18777..19124)
                     /locus_tag="PLJ82_01860"
     CDS             complement(18777..19124)
                     /locus_tag="PLJ82_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40288.1"
                     /translation="MKTKTYLQQLVTSLKVNKFYLFPYLIVWLMGLLVVLLYDKIDIH
                     QFTNQRPFAVGDALFPYITKLGETFPFIASGALLLYKVRKALFVLSVQVVGAIVVYTL
                     KNLFRAPRPRIVF"
     gene            complement(19128..19772)
                     /gene="mnmD"
                     /locus_tag="PLJ82_01865"
     CDS             complement(19128..19772)
                     /gene="mnmD"
                     /locus_tag="PLJ82_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOR40289.1"
                     /translation="MQIITTSDGSHSVYSPQFDEAYHSSFGAMQEAEYIFINKGLLYT
                     AKTSLQVLEIGFGTGLNAFLTSVYGNNISIHYTGIERFPLSVEEANQLNFASFFPEQK
                     AWFTGIHQAEWGSFQRINENFQLCKVQADVLHIDFAKEQYDVVYFDAFSPEKQPEMWT
                     ESVFTRIFEACKPGAVFTTYCCKGYVRRLLQGVGFTVERLPGPIGKRQILRAIK"
     gene            complement(19836..20411)
                     /locus_tag="PLJ82_01870"
     CDS             complement(19836..20411)
                     /locus_tag="PLJ82_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR40290.1"
                     /translation="MKSIKGTKTEQNLLISFAGESQARNRYTFFASQAKKEGFEQIAA
                     IFTETAEQEKEHAKRFFKFLEGGMVEITASYPAGVIGTTAENLLAAAQGEHEEWSKDY
                     PLFADIADEEGFPEVAQTFRAVALVEEMHENRYLAFWKNVKEGKVFVKDEEVVWQCRN
                     CGYIVSSNEAPDECPACKHPQSYFEVMKTNI"
     gene            complement(20429..20947)
                     /locus_tag="PLJ82_01875"
     CDS             complement(20429..20947)
                     /locus_tag="PLJ82_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOR40291.1"
                     /translation="MKTVTITEQPKIDEVIRSCQFCFVAVADIDATPYVFPMNFGYQD
                     GILYLHSGPQGRKTDILEKNPKICISFCANASLYYTAEQIACTYLMQTKSVIIDGQVE
                     FIADDAEKIRIMNIIMAHYTAKTDFQYSDPAIKNVRVWKVQPTQVTCRFLGVPHREAM
                     QLEKTQHIKKNV"
BASE COUNT         7420 a         3746 c         3801 g         6445 t
ORIGIN      
        1 tggcagaatt caaaatcatt gagcctacta caaaggttgt tcttacaaca caaaatatcc
       61 catatggagc caaattgtat ttccaatcag gagtaaccgt tgaaaaaggg gctgtaattt
      121 gcgaatggga tccttttaat gctgttgtta tatctgaggt tgctggtaaa atgacgttcg
      181 aaagtatggt tgaaaatatt acattcaaaa ccgaatctga tgagcaaact ggcttgaaag
      241 aacgcatcat aattgaatcg aaagataaaa gcaaggttcc tgcggcacat atacttgatg
      301 aaaaagggga aattttacga acatatgcac ttcctgttgg tgcacacatt gcgattacag
      361 aaggttctaa aataaaaatt ggacagattt tacttaagat gccacgggca gtaggcaaat
      421 ctggagatat cacaggtggt ttgccacgtg taacagaatt gtttgaagca cgtaatccat
      481 ctaatccagc tattgtctcg gaaattgatg gtgaaattac atttgggaaa attaagagag
      541 gtcagcgcga aattgtagtt acatcaaaat ctggagaagt aaaaaaatac ttagttcctc
      601 tgtcaaaaca aattttagtt caagaaaatg attttgtaag agctggtaat ccattatcag
      661 atggagctat tactccttct gacatattag caattaaagg acctacggct gtacaagagt
      721 atattgtaaa cgaagttcag gatgtatatc gcctacaagg agttaaaatt aacgataaac
      781 actttgaagt aatcgtgcgt caaatgatgc gtaaagtgga aatagaagat ccaggtgata
      841 cacgtttctt agaaaaacaa actgttgaca aacaagagtt tatggaagaa aacgaccgta
      901 tatggggtaa aaaagtagta gtaaacccag gtgattctga ggtattaatg gcagggcaaa
      961 ttattgcacc acgtaaatta cgcgatgaaa acagtatgtt gaaacgtaaa gatttaagaa
     1021 cagttgaggt gcgtgatgct atgagtgcaa cgtcaagtgc tatattacaa ggtattacta
     1081 gggcttcgtt acaaactaca agctttatgt cagcagcctc gttccaagaa actactaaag
     1141 tattaaatga tgctgctata aacggtaaag tggacaagct agaaggatta aaagaaaacg
     1201 tgatttgtgg tcatttaatc cctgctggaa caggacaacc agaactcgaa aaaattgtag
     1261 tagcttcgcg tgatgaatat gaaaaaatta tgcaagctaa acgcagtgta acagatgtga
     1321 atctaaaaga agcagaagaa gtttctttct aacaatcata tctccttcat ataaaaaagc
     1381 atcctatttt gttataggat gcttttttat tgatattctt ttatgtgtta tagaatctat
     1441 gggaataatc aaaaataaca agaccttatg aatcaaaaac aataccaaga ttaaacttga
     1501 tatatcaatc atttacctat acgtgtttta tattcttcaa atttattttc aacaaattct
     1561 acatagttag ctgtttcgtc tcctctgaga tagccatgtt tacatacttc atcggtatag
     1621 tattctgggt tggactttaa ccttacataa atctctacat tatcgtgcca gatattggga
     1681 tctttgccat atttaagagc taatgcccga gcatcaaata aatgaccagg tccagaatta
     1741 tatgcagcta atacaaactt tattctttct tctttaacag gaatttgttg gaagattccc
     1801 tgtaaatgtt gtaaatactt aactcccgct tttatacttt gctctggact atgtaaatca
     1861 tgttcactaa aacccattgt acgtgcagtt gatggcatca gttgcataat accagcagca
     1921 cccgcccaag atacaatggt aggatcgaat tttgactcct tgtatgcaat agcagcaatt
     1981 aaacgccagt cccaagaaac aatggatgca tatttcttaa aataatcatc aaaggcagaa
     2041 agttgatgta gttttttttg ttcaaatcct tcagactcaa aatatttggt acgttgaaaa
     2101 tacttattat aaatactcga caacttcata tttttagcat ttttagcatt ccacttgttg
     2161 atatatgcta acaattcagg attatcattt ctcacagccc aagcataacg ttgtggaaaa
     2221 ctgatagata aagaataatc aattgaagaa aaatatgttt tattcaatct agcaatatca
     2281 gcatcggcaa ttgtcattgg aataagctgt tgtgctgttt gtacgataag ggcatctaca
     2341 gatacagaat cattaactgc tacaatgtgt atacctcccc ctatttcatt attcaaatcg
     2401 acaattctat catgatagat tgatgattca ggcacatata cttcttttcc agctaattgt
     2461 agtacatcag taagtatgct atctgattgt aattgtataa caacctgaga tgttacatat
     2521 tctctatccg taaagcttac acgttgttga tactccaatg ttcgtggaac acgataagca
     2581 attaaatcgc cctctccatt ttccaagagt tctatcatgt tggcaatatt tttagcaaca
     2641 atcacctcca tttctacacc caattcttga cttaattgtt gtgcaaactc atattcgtac
     2701 cccattacat tttccttata gttaaaatac gatagaggac catataacgt aataacacga
     2761 atctttccac gttgcaaaat accctctaaa tctggaatat cttcttgatt tccctggttt
     2821 tctccactaa tacaggatac gcaaattatg gaaagaaaaa taaatagcaa taaatagata
     2881 cattttttca ttcatcttta ttttataaac atttaaacca attgtatgtg tttatcttgg
     2941 atgataggtt ctccaatgag ttttagatat acttgaattg ttgccacgac atctttctga
     3001 caatagattg ctattctatt tatatctcca tgttcgtaat acacagttgc tacctcgctt
     3061 ccatcaatat cgtctttggg agtgggaata ttaaaaattg ctgttaaaag ttttaatgag
     3121 gtataattct tataatctcc aaaacgccat agttctaagg tatcaagaaa cttaacctcc
     3181 cacggttttt ttccagccac ttgcaataca tcaggcaatg gtaagccatt aattaacatt
     3241 cgtcgagcaa tatatggcac atcaaactct ttgatattat gaccacataa ttgatattca
     3301 gccgattgca taaattttaa taacattcct gagaattcgg ttaagagcgt tttctcatca
     3361 tgcccaaaaa atgacttaat acgacaatgt ttttcgccat cttttaggta aatgattccg
     3421 acagaaatgc aaacaatttt tccaaattca gcatatatac cagcttcttt tctatacaaa
     3481 tcatcaatag agctatcatc atcataccta tcaggcattc tacgttttag ggtatcaagt
     3541 ttatccatcc acaaatgagc taaggactca ggcatctttt ttatagttgc ctcttgggga
     3601 acagtttcta tatccaaaaa taatatcttt tcaagtgttg tttgttgcat atcatttatc
     3661 ttaatttgtt gtctatataa tgttgtaaaa tactgatagc tactttattg tgtcctccct
     3721 ctggaataat taaatctgca tatcgtttag ttggttcaat aaattgtaaa tgcattggtt
     3781 ttaatacttt ttgataacgt tctattacag aattaaccga acgtcctctt tctataatat
     3841 ctctattaat aaccctaatt aacctatcgt cggaatctgc gtcaacaaat acttttaaat
     3901 ccattagttt tcgtaattct ttatcggtaa aaatcagaat tccttccaca acaataatct
     3961 ttgcgggttt tataagtact gtttcttcag cacgagagca agtaatatat gaatagatag
     4021 gttgctgaac agaatttcct tttttcaatt ccttgatatg ggaaattaac aaatcaaatt
     4081 cgatagaatc tggatgatca aaatttattt cgatgcgttt atccattggc aaatgactac
     4141 tatccttata ataagaatct tgtggtaaaa aagcaatgtc gccatcaggc aattgactca
     4201 atatttcctt tgctacagta ctttttccag aaccagttcc gccagcaatt cctataatta
     4261 tcatattttc agtatattaa attaagaatg cactaaggta gaaaaatatt tttaagaaag
     4321 ataaaaaaag atgcaaaaaa aactattttt tatttatctt tgcaccgatt taattaacaa
     4381 taataaattt acttaatcta gaaaaaagat gtcagttaac aaagtaattt tggtcggaaa
     4441 tgtgggcaaa gaccctgaag tacgttattt agagaacaat gttgcagtag ctaaattttc
     4501 attagcaaca acagaaaaag ggtataagat gaataatggc accgttgtgc ctgatagaac
     4561 agagtggcat aatattgtta tgtggagagg attagctgaa attgctgaaa aatttgtaaa
     4621 aaaaggctct caattataca ttgaaggaaa aattcaaagt agaacatggg atgataagaa
     4681 tggtgtaaaa cattatgcaa cagaaatttt gtgcgaaaat atgcaaatgt taagcaaaaa
     4741 atcagaagca gaaagtggaa atgctccttt aaacgacaaa tctgtaagca ccaaagcaga
     4801 ggaaactcct tttggcaata gcgacaacat cgcagatgac gatttgccat tttaattcag
     4861 tcattcacta gcctaaaaaa aaagacagag caattatttg ttctgtcttt ttttatcaac
     4921 tctttttgag ataattttat aatgaactat tgttcaatcg ttgagccata ctttcagcca
     4981 attttacgca ttccacgtat ttatcgtgct ttaatgcaaa tttttgttct actccacctt
     5041 cgtatactac ctcaaaacca acttgttcgg caaatgcagc gagcttctta ttggcaacac
     5101 ctgcccacga aaacgaccca aaataagcaa aatatctgtt tttaatatct ctatttttaa
     5161 ttttactcaa cattgattcc accaaaggga atagttcgtt tgagtatgta ggactaccaa
     5221 taatcaagcc ttgataccta aaaatatctg caataatata cgagggcgtt gattttgaga
     5281 cattatgcat taccacattc tcaataccat tggcaactaa agtatcagca ataacttcag
     5341 ccatttgctc tgtatttcca tacatacttc catacatcac caccactcct ttttttcctt
     5401 gatacaaact taaatcatta taaatagata tcgttttttg tagatatttt tgccaaacag
     5461 gaccatgtgt actacatata gtttgaatat ctaagttact tagtttagct aatgcacgtt
     5521 gcactggata accatattta ccaacaatat tggagtaata tcgctccatt tctgtccaaa
     5581 atttatccac attaatttgc gaatcaagca cccctccgtc caacgcacca aaacatccaa
     5641 aagcatctcc agaaaacaat actttgttcg tttcatcaaa cgtcatcatg gtttctaccc
     5701 aatgcaccat gggtgtaagg taaaaacgca aaacagtttt tcccaatgtt aaggtatctg
     5761 cttcttttat ttcgtacaaa ttatctttaa tattaaaaaa gccatctaac atgccaaatg
     5821 ttttggcatt ccctacaatt tgaatggaag gatattttct cttaatcgtt gcaatagaag
     5881 cagaatgatc tggctccatg tgattaataa tgaggtagtc aatgggtttt tcgcccaaaa
     5941 taccctctat ttttgacagg aataaatcag aataacaagc atccaccgta tctactaacg
     6001 caaccttttc gtctacaatt aggtatgaat tatatgatac gcctgcggga atctcccata
     6061 aactttcgaa taaatgcttt gttctgtcgt ttacaccaac ataataaata ttgtttgtaa
     6121 tttgtttagt agtatgcata acaaaatttt tatatgtata ttaaagtgca aagataaaaa
     6181 aaatatgcta cacaactact tgataaaatt gaagatagcc acgatataac attttttata
     6241 tgacattctg atatttaaga gtgtttaacc cttgtgtatt gtaaaagaga tgtattttat
     6301 atatctttgt aagttagttc ttatctcttg ataagattct atttttttac agtaaataaa
     6361 ttaacaaaga taggtttcga gatcatctta taacaacaaa aatagttcga tgcaattttc
     6421 gcaaattatt ggacaagaca taattaaaca acaacttatt acgagtgtac gtgaagggag
     6481 aatacctcat gcacaattat ttttggggca agaaggagtt gggaagctag cattagctat
     6541 tgcatatgcc caatatgtgt cttgtttaaa taagggagaa cacgattctt gtggcacatg
     6601 ttcatcatgc ataaagtatg aaaaactcat tcatcctgat ttacacatgg tattcccttt
     6661 tgcaaaagat ggtaaaacaa aagaaatttg cgatgattta ctccccgaat ggagagaatt
     6721 tgtaacgaaa aatgtttatt ttagcctaga acagtggatg gataatattg gagcaggaaa
     6781 taaacaagct actatttatg caaacgaaag taatgaaata ctacgtaagc tccaattaac
     6841 cacatacgag tctgcatata aaataatgat tatttggttg ccagaaaaaa tgaacattac
     6901 gtgtgctaat aaattactaa aaatactcga agaacctccg acaaaaacat tatttatttt
     6961 ggtaagtgaa caagtagaaa aaattatttc taccattata tctcgtacgc agatgattaa
     7021 aataccgttg attgagacac atacaatgag ttcgtactta caggcaaagc actctatttc
     7081 gaaagaaaat tctatcaata ttgctcgaat tgcaaatggc aattatatta aagccctttc
     7141 gatcatcaaa gcaacggaag aatcgcaact taactttact cgctatcaag aaataatgcg
     7201 ctatgcttat aaaaatgatg tgctagcaac aaaaaaatgg gcagaagaaa tgacatcgtc
     7261 ctctatgggt agggaaaaac aaaaaagttt tctcgattat gctcaacaca ttacgcgcga
     7321 atgttttatt aaaaatatag aaataaacga actaaattat ttaattgatt atgaagaaaa
     7381 tttttcttct ggttttgcac gatttgtaaa cgaaaaaaat attcaagcat tgatggattt
     7441 ttttgcagat gcagcaaccg atattgagca aaacgtacaa gggaaatttg tattttttga
     7501 tttgggatta aaattaatga ctcaaatcaa acagaactaa ataaacaaac ttatggaata
     7561 tgaattagcg aaatcaacct gtggcattaa agtgtgcggg tgcaaaaaaa actctctgca
     7621 aatgctgagt gtttacgact ggctttcgga tatacccgaa acacagataa attgtaattt
     7681 tgtagaagta caattcaaaa acacgagaaa aggatattat cttaattcga ccaatataaa
     7741 actagaaaaa ggagatattg ttgctgtaga atcaacccct ggacacgaca taggaacagt
     7801 aaccctagtt ggcttgttag tagacagaca aatgcgaaag aatggcattg accctaaaac
     7861 cattgaagta agacgggttt atcgtaaggc aaagcaaact gatttggaaa agtgggaaga
     7921 atctaaggca aaagaacacg atgccatgtt aaaatctcgt caaattgcac aagatcttaa
     7981 actagatatg aaaattggcg atgtggaata tcaaggagat gggaacaaag caatatttta
     8041 ttatattgca gaacaacgcg ttgattttcg tcaacttatc aaggtattgg ctgaaacatt
     8101 tcaagtgcgt atcgaaatga aacaaattgg agctcgtcaa gaagcaggac gtattggagg
     8161 tacaggtccg tgcggtagag agctgtgttg ttctactttt ttaaatagtt tttcatccgt
     8221 tggcacacac gctgcacgtg tacaagattt atcattaaat ccacaaaaat tggcaggaca
     8281 atgtgctaaa ctaaaatgtt gcttaaatta cgaactttct acctatacag aagctttaaa
     8341 agaattccct tcaagggaca ttcaactaga aggagaagat tgcacctatt atttctttaa
     8401 attagatatt tttgaaaaaa agatggcata ttctcaagct ccaaattttc caaaaaatgt
     8461 agttgaatta tctctggata aagtaaagga aataattgct ctaaataaaa aaggagtacg
     8521 tcctgctatt caaggcaata tcaaagaaac aaagaaagag gaagaagaaa ctaaaagttt
     8581 tgcaactgac ttagaagaag gaagtttaac ccgttttgat aacaaaaaat caaaaaaacg
     8641 aaataataaa cgtagaaaac ataaaaatgc atctaccaat gaaacaaata cttcatcaca
     8701 gcaataatct gctaattgcc ttatcggtgg ggctgctttt ttctttacaa agttgtgggg
     8761 aaaaatcagt gtataatgag tacaataaaa tagctactgt tggatgggat tttggcaccc
     8821 ctgctcgctt tgaaactgaa atcaacgata cactgtcagc atatgacatt acgattcaag
     8881 tacgtaataa cacagattat gcgtatcaaa acttttggat ttttgtcgaa tcaactagcc
     8941 cttctggtat tgtagcaaaa gatacattgg aatttttatt ggcagacaat acgggaaaat
     9001 ggttgggaaa aggcataagc tctacgaaag aaatatctgg cttatatttg gaaaatatta
     9061 aattcccaga gcttggcacc tattcttttt ctctttacca aggaatgaga agtgcatcac
     9121 taaaaggaat agaaagcgta ggattacgca tagtaaaacg tcaataatat gggaaaaaat
     9181 aagttgaaaa aatttgagga catggactct tacccacatg tgtttcagtt ccctttcagc
     9241 aagattcaag aacaaggatt tcacttaaaa aatcattggc atacgaattt ctttcagaat
     9301 aatcatccaa ttgtacttga attgggatgt ggcaaaggcg aatatacggt agaattggct
     9361 aaattatttc ctgaaaaaaa ctttattggc attgacatta aaggagcacg tatgtggacg
     9421 ggagcaaaag cttcgcttga acaaaagatg aacaatgttg cttttattcg tacaaatatt
     9481 gaattgatta cacatttttt tggcacaaac gaagtgtcag agatatggat aacctttccc
     9541 gacccgcaaa tgaaaaaagc gagcaagcga ttaacatcta cccgctttat ggcactgtat
     9601 aacgagattc tacagccaaa cgggctgatt cacctaaaaa cagacagtcc attcctgtat
     9661 acctatacac aattaatgat tgaaaaaaat gcattagaca ttcattttca gactgacgat
     9721 ttgtatcatt ctaattgggt taacgaaatc ctttccatcc aaacttttta cgaaaaacaa
     9781 tggatagaga gaggattgag tatcaaatat atccaattta aaataaataa aaataccccc
     9841 ttgctagaac ccgaagaaga gatagaacac gactcttaca ggagttttaa tagaagcaaa
     9901 cgaagtatgt aatctactaa ttaaacagat aaaaccatgg ctatatatcc acaactcatc
     9961 attgatgcac ttagaaaagt gcgttatcct ggtaataata aagatattgt tacgaatgaa
    10021 atgcttgaag atgatattcg tattgaagga aataaagtgt cattttctct gatactgaaa
    10081 gcgaatgatc cgtttgcaaa atcattagta aaagcggcag aacaagctat attaacctat
    10141 gtaggcgaag aaattgatat caaagggaat attagtatca aagcactacc tgctcctaac
    10201 ccgatgttaa atccaagaag tttaacccat attaaacaca aaattgccat ctcttcagcc
    10261 aaaggaggtg ttggaaaatc aactgtttca tcaaacttag cagtagcatt ggctaataaa
    10321 ggatacaaag taggattgct tgatgcagat atctatggac cgtccattcc aaaaatgttt
    10381 gacattgaag aagaaagacc aactgtagaa gttattgatg gtaaagattg gattgtcccc
    10441 gtagaaaaaa tgggtattaa aatcctctct attgcctttt ttatagaaaa agataatgcc
    10501 attatatggc gtggagcaat ggcgagcaat gccctgaaac aacttataaa tgacaccctt
    10561 tggggtgatt tagatttttt cttaatcgat ttaccaccag gaacaggcga tgtacacctt
    10621 acgcttttgc aaaccatcga tttaaacgga gccattatcg taagtactcc tcaacaagta
    10681 gcccttttgg atacagtgaa aggcataaac atgtttttaa atgaaaaaat aaatattccc
    10741 atagtaggtt taatagaaaa tatgtcatgg tttacaccag cagaactgcc aaataacaaa
    10801 tattatattt ttggaaaaga aggaatgaaa aatcttgctg aaaacatgaa attacctttt
    10861 cttggacaaa tacctattgt tcagagcatt tgcgaatcag gagatagtgg ttctcctatt
    10921 attattaacg aaaatagtat tgttggcaca agttttgata caattacaga aaacatctta
    10981 cgtaatctca aaaaatagag gtaattaaaa cagaaatatt tcattatcca tactttacaa
    11041 ctatatttgc agaaaaatta accatatgac agatacacaa caattagtaa acagcataat
    11101 tgccggaata caagaaaaaa aaggctcaaa aatcgtagta gctgacctct caaaactaag
    11161 taacagcatt tgttcctatt ttatcatttg cgaaggggga tcaaataccc aagttgatgc
    11221 cattgcaagt tcacttaaag actacgtaaa aaaagaaaca aaagaacgac cattgggcat
    11281 ggaaggttta gaaaattgcg aatgggtggt aatggattat ggtgacgtaa tggtacatat
    11341 tatgcaacgc gaaccgcgta aattttatga tttagagaat ttatggcaag atgctaaact
    11401 aacagaaatt gctgatatat aaaaaaatca atattgatta agattatttg gaaaaaacat
    11461 ggaaaacgaa aataaacaag gcaaacaacc aaacaataat aaacccaaag ggaaatttat
    11521 tgtaaatatg tattggtttt atggtataat tgcagccgta ctaattgctc taaatatatt
    11581 tatgaattcg gatgcaagta aagaagtatc ttattctgaa tttagaaatt atgccgaaaa
    11641 gggatacgta gaatcgattg ttgtaattac caataagaat gctgctgaag tagtaatcaa
    11701 acaagactca tcaattttaa acactgtttt tggcgatgac aaaggtaaag taaactcatt
    11761 aattgtaaca attccttctc ccgaaaaaat ggaagaattt gtagcagata atgacaagaa
    11821 tttaaacata aaaacagaca taaaatacga agaacgtaag aattatcttg acaccttact
    11881 ttggtcgttt ggtccattta tactcatcat tataatttgg attttcatca tgagaaggat
    11941 gagtggtgga ggtgctggag gcaacgtgtt taatgttggt aaatcaaaag cacaattatt
    12001 cgacaaagga gctaacacaa tgtcggtaac gtttaaagat attgctggat tagccgaagc
    12061 taaacaagaa atacaagaaa ttgttgactt tttaaagaat ccttctaaat atacaaattt
    12121 aggcggaaaa atccctaaag gagccttact cgtaggacct ccaggaacag ggaaaacact
    12181 tcttgccaaa gcagttgcag gagaagctca cgtaccattt ttctcgatgt cgggttcaga
    12241 ctttgtagaa atgtttgtag gagtaggtgc ctcgcgcgta agagacttgt ttcgccaagc
    12301 caaagaaaaa gctccgtgta tcatttttat agatgaaatt gatgccattg gtagagctag
    12361 gggaaaaaac ccatctttca actcaaatga cgaaagagaa aacacactga atcaactact
    12421 tacagaaatg gatggctttg gctcaaatag tggggtaatc attttggctg caactaatag
    12481 agctgaaata ttagacaaag cactgatgcg agcaggacgt tttgaccgac aaatacacgt
    12541 ggacttacct gacgtgcatg agcgtaatga aatatttggc gtgcacttac gtccgttgaa
    12601 actgtcgccc gaactaaaca tcgagttttt agcaaaacaa acacccggtt tttctggggc
    12661 tgatattgct aacgtatgta atgaagccgc attaatcgct gccagaaaaa acaaagaagt
    12721 agtagaaaaa caagactttt tggatgcagt tgatagaatt attggaggta tggaaaagaa
    12781 aaataaaatc accactaccg ccgaaaaagc atctattgcg actcacgaag ctggacatgc
    12841 cgctgtaagt tggttgcttg aatatgctaa tccattggta aaagtaacca ttgttccgag
    12901 aggcaaagca ctaggagcag cgtggtattt gccagaagaa cgacaattaa ccatgaaaga
    12961 gcaaatacta gatgaaatgt gtgccatatt aggtggtaga gccgccgaag atgttgtatt
    13021 taaccgtatc tctactggag cattaaacga cctagaacgt gctacaaaaa tggcttattc
    13081 tatcgttgta tattatggaa tgagtaataa attatccaat gtaagttatt ttgactccac
    13141 aggaggagaa tattctttta caaagccata tagcgaaaaa actgcagaaa ttattgatac
    13201 agaagttcaa tcaataatag cggagcaata tgaacgagca aaaaaaatca tttctaacaa
    13261 caaagagaat catgcacaac tgtctcaatt gttgattgaa aaagaagtaa tatttgctga
    13321 agatatggaa caaatattcg gtaaacgtcc ttgggcaagc cgtatggatg aattaattag
    13381 tgaaagtgaa aaaaataaaa taaatacatc aactactgaa gcaacaacga ctctatgatt
    13441 aaaaatttca tacaacggac tcttacgggg tttatgtttg ttgcaagtat cttgcttagc
    13501 atttactttc aagctactta ttatacattt tcaatcttat tttttggatt tacggcaatt
    13561 gctctatttg agttttaccg aataataaat caatacaaag aagtaacatt aataacaggg
    13621 ctaccgattg caacgggttt gattttatac ataagtacat ttttacttgc agctggaatt
    13681 gctcactatt caatccttgt gttttatgca gttggtattg taggagttat aatagctgag
    13741 ttataccgaa agcaagccaa tccatttcac aatattgcat ttatcgtgct gggtcaattt
    13801 ttcatagctt tcccttttag catcctcaat gcaattgcct ttgcagaaat atattggtta
    13861 tttgcattat ttatgttaat atgggtatat gattcatgtg cttatatggt aggagtaatg
    13921 tttggtaaac accgtatgtt tgagcgtatt tcaccaaaaa aatcatggga aggatttatt
    13981 ggtggatttg tattaacttt ggctgcaaca ttaatttttg cctattttac acctgatatt
    14041 gccatatgga aatggttatt atttggagta ttaatagtta tatttggaac atggggcgac
    14101 ttaacagagt ccctattaaa aagaacagta gacatgaaag attctgggaa catacttcct
    14161 ggacatggag gaatacttga tcgttttgat agtttgttat tattggctcc tattatatat
    14221 tttttcttac aactaattta aaaaaatact atgataaaaa tccacaaaga aggacgaatg
    14281 ttgctccttg ttcttttttt agccctaacg attgttaata cgctgttgtt tttcattttt
    14341 gacaatgcta tagtacctac aattgccctt gttgtttcaa taggtttttt tattttcttt
    14401 acgcatttct ttcgcagtcc tgaacgcatt gtgttagcag acgaacatta tgtggtggca
    14461 ccagccgatg gcaaaattgt ggtaatagaa ccagtcgaag aacatgaatt tttaaaagaa
    14521 aaacgtttgc aagtatcaat tttcatgtct ccttttgatg tacatgccaa ctggtatcca
    14581 atagcaggta aagttatcca tgtagcccat catgatggac gtaagatggc tgcctattta
    14641 ccaaaatcga gcacagaaaa tgaacgttcg acagtagtga ttgaaacaca acataagcaa
    14701 cttgtactta tgaggcaaat agcaggagct ttagccagac gcattgtaac ttatgcgaaa
    14761 gtaggcgaca attgcaagat aaatcaacag tttggattta tcaaatttgg ttcgagggta
    14821 gatttgtatt taccacttga ttcagaaata ttggttgact taaacgaaga ggttgtagga
    14881 aacgaaacca tcatagctaa actaaaacac gaagtataat ctactctaaa cacagactta
    14941 attagtcgag gttagaccct aaaatattct catgttagta aaaacatttg gtgccgcagt
    15001 gcatggtata gatgcttcca tcattacaat tgaagtgaac gtatcgcaag gtataaagtt
    15061 ctttttggta ggactccccg ataatgctgt aaaagaaagc cacgaacgga ttgtatccgc
    15121 cttgcagtac aacgggcata aattccctcg ccaacaaatc atcatcaaca tggctcctgc
    15181 cgatatccgc aaagaaggtt cagcatacga tttacctttg gctatgggta ttatggcggc
    15241 agccgagcaa atagcctgcg aaaaaataaa tgaatattta ttgatggggg aattatctct
    15301 agatggcaat attatgccca tcaaaggagc cttacccatt gccattaaag cacgagaagc
    15361 tggatttaaa ggattgatcc tgcccaaaca aaatgccaaa gaagcggctg tagtgaacaa
    15421 cctcgaagtg ttaggtgtcg aaaatattca agaagtaatt gggtttttaa atagcgaaac
    15481 aacgatagag ccgaccattg ttcatactag agatgagttt tattccatgg ctagtgattt
    15541 tgaaatggat ttttcggatg taaaagggca ggaaaccgta aaacgggcta tggaagtggc
    15601 agctgcaggt ggacacaata ttattatgat tggacctcct ggagctggta aatcgatgtt
    15661 ggcaaaacgc attccatcta tcttgccacc acttacctta cacgaagcat tagaaacaac
    15721 aaaaattcat tccgtagctg gcaaaattga aaaaggtata tcgttgatgt acaaacgtcc
    15781 gtttcgcagt ccgcaccata ctatttctga tgtagcacta gttggcggag ggagttttcc
    15841 acaaccaggc gaaatttcgt tggctcataa tggggtattg tttttagacg agctacccga
    15901 atttaaacga accgtattag aggtaatgcg tcaaccactc gaagatagaa aaatttgcat
    15961 ctcacgctca aaatttagca ttgagtatcc agctagtttt atgttggtat catcaatgaa
    16021 tccatgccca tgtgggtact ataatcaccc ggagaaagat tgtgtatgcg gacctggtat
    16081 tgtgcaaaaa tacctcaacc gcatttcagg accactgctc gaccgcattg acatacacat
    16141 cgaaattgtg cctgttcctt tcgaaaagtt gtcatgtacc aaacctacag aacacagcga
    16201 agtaatacgc gaacgtgtaa taaaggcacg aaaaatacaa gaggaacgtt ttaaagagaa
    16261 tgatggcgta cactgcaatg cacaaatgtc atcgaaattg ctcagagagc acacaaaact
    16321 caacgatacc agtcaacaat tgttgaaggt tgccatgcaa cggctcaatc tttcagcaag
    16381 agcttacgac cgaatattaa aagtagcccg aacaatagcc gatttagatg atgcaaagga
    16441 tattcaagta gaacatatat ccgaagctat caattatcgc aatctcgaca gagaaggttg
    16501 ggcaggttaa ataaaaaact aaaaaaaatt atatactatg gaaacagtac ttagtggcat
    16561 taggtcaact ggaaatttac atttaggaaa ttattatggt gctttacgca attttataaa
    16621 aatgcaagaa gaataccaat gtttcttttt tatagctgat taccactcgc ttactacgca
    16681 tccaaaccca cacgaaatgc atggcaacgt acgcaatgtg ttgactgaat acctagcagc
    16741 gggcattgac cccgacaagg cgactattta cgtgcaaagc gatgtacctg aaattcctga
    16801 gttatacctt ttactcaaca tgcatgccta tttgggagaa ctctcaaaaa cagcctcatt
    16861 taaggaaaaa gcccgcaaac aaccagaaaa tgtgaatgct ggattattaa cgtatcctac
    16921 cttaatggca gcagatattt taatacataa tgctcacaaa gtgcctgtag gtaaagacca
    16981 agaacaacac ctagaaatga cacgcaaatt tgctcgcagg tttaataatt tctatcaagt
    17041 agaatatttt agagaaccag ctgcttttaa ttttggagaa gacttgctca agattcctgg
    17101 tttagacggg agtggaaaaa tgggaaaatc agaaggaaac tgtgtatttt tggctgatga
    17161 tgccaaaaca atagaaaaaa aagtaaaaag agccttaaca gatgctggtc cgacgacacc
    17221 caactcaacc aaacctgatt atattgaaaa cctatttact atcttaaaag tagtttctac
    17281 acctgaagta gtaacacatt ttgaagagaa atggaatacc tgcgatatcc gttatggaga
    17341 cctaaaaaaa caactagcag aagatattat tcgtgctact tctcctattc gcgaacgcat
    17401 cattgatata caaaacaacg aagcatacct ggcaaaagta accaaagaag gagcagaaaa
    17461 agcacgcata agtgccgcta aaacattacg cgaagtaaga gaaataatgg gattcaaaaa
    17521 gttttaatat gccaaaagtt gttggaatag gcgaaactgt atatgatata atttttgaaa
    17581 acaatcgtcc gatagatggc aaaccgggcg gttctgtgtt caatgcactt atttcgcttg
    17641 gtcgattgca acaacaaacc gcttttataa gtgaagtagg cgatgatact gttggaaaaa
    17701 taattctgga tttcttggct caaaacaaag taaatgcgga ttatgtagca caatttgatg
    17761 gcactaaaac ggctttatct ttagcttttt tagatgaaaa caaaaatgca aactacacct
    17821 tttacaaaga ttattctgct caaaaagctc cacataaaac accgcccatt acaagcaagg
    17881 acattgtatt gtttggctca ttctttgcct taaatagtac tgttaggcaa caagttacaa
    17941 cattacttga taccgctaat aaagagagtg cgatggtgta ttacgatgta aattttagaa
    18001 aaacacacat cagcaaacgc cacgaattac tccctgcttt gattgaaaat atgaagtatg
    18061 ccgatattgt aaaaggttct gaggaggatt ttgatcatct ttacgatgaa aaagacccaa
    18121 taacgattta ccaaaaatac attgctcccc attgttcaac gtttatttat acacaaggag
    18181 ctaatggtgc aatggtattt acacccacct attcgctaca cgtaccttct atacccattg
    18241 ttccaataag cactatcggt gctggagata cttttaatgc tggaataata tttgccctgt
    18301 tgcaacagaa cattgcaaaa gatactttat gcaatgtcca acagcacgaa tgggaagcca
    18361 tcctacaaca agcgattgat tgtgctacac atgtgtgtat gctctacgat aattacatca
    18421 ccgtagattt tgccaataaa aaactggatt tatttcgttg atttatttgc taaagacatt
    18481 agccctttgt ttccccatgt ttgttgggta ttaaaaaatg ggtacatcgt caatgcaatc
    18541 acaacggcaa gtaaggatcc tgctaaggta tcttctaaaa aatgctggtt caaataaata
    18601 cgtgaaaaac cgaccaacaa cgacatggca aaaaagaaga atttgagtag ctgatttttt
    18661 acaatcaagg caagtgataa gaaaaaagca aatgctgcca ttgtatgacc agacgggaaa
    18721 ctgttccacg catgcaaacg aacgccatca atcgtatgta aatccaagcc taactcttaa
    18781 aaaactatgc gaggacgggg agccctaaac agatttttta gcgtatatac cacaattgca
    18841 ccaaccacct gaaccgataa tacaaaaaga gccttgcgta ctttatacaa taacaatgca
    18901 ccgctcgcaa taaacggaaa ggtttctcct aatttagtaa tatatggaaa caacgcatca
    18961 cctacggcaa acggacgttg attggtaaat tggtgtatat caattttatc atacagcaac
    19021 actaccaaca aacccattaa ccatacaata agatacggaa ataagtaaaa tttattgact
    19081 tttagcgaag taacaagttg ctgtaaataa gtttttgttt tcatatttca ctttattgct
    19141 ctcagtattt gacgctttcc tattggtcct ggtaaacgct ctactgtaaa cccgacccct
    19201 tgcaacaacc tacgtacata gcctttacaa caataagttg tgaaaactgc acccggtttg
    19261 catgcctcaa atatacgtgt aaaaactgat tctgtccaca tctcgggttg tttttctggc
    19321 gaaaaggcat caaaatacac tacatcatat tgttctttgg caaaatcaat atgcaacaca
    19381 tctgcttgta ctttacacaa ctgaaaattt tcgtttattc gttgaaaact accccactct
    19441 gcttgatgaa tgcctgtaaa ccaagctttt tgctctggaa agaaacttgc aaaattcaat
    19501 tgatttgctt cctctactga tagtggaaaa cgttcaatac ctgtataatg tatggatata
    19561 ttgttaccat aaacacttgt taaaaaagca tttagacccg taccgaaacc aatttcaagt
    19621 acctgcaagg atgttttagc agtgtacaat aagcctttat taataaagat gtattcagcc
    19681 tcttgcatgg caccaaacga cgaatgatac gcctcatcaa attgtggcga atataccgaa
    19741 tgcgaaccgt ctgaagtagt aataatttgc atatagtaaa aaaacaacaa tcgccacaaa
    19801 ataatgtagc gattgaatat tctaagatat tttttttaga tatttgtttt catcacttcg
    19861 aaatacgatt gtggatgttt gcaagctgga cactcgtctg gtgcttcgtt agagctaacg
    19921 atataaccac aattacgaca ttgccatact acttcttcgt ctttaacaaa tacttttcct
    19981 tcttttacat ttttccaaaa tgcaaggtaa cgattttcgt gcatttcttc taccaatgct
    20041 actgcacgga aagtttgtgc tacttcaggg aaaccttctt cgtcagcaat atcagcaaat
    20101 agtgggtaat ctttcgacca ttcttcgtgt tcgccttgag cagcagccaa taagttttcg
    20161 gcagttgtac caataactcc tgctggatac gatgcagtaa tttctaccat accgccttct
    20221 aagaatttaa aaaaacgttt agcgtgttct ttttcttgtt ctgctgtttc ggtaaaaatg
    20281 gcagcaattt gttcgaaacc ttcttttttg gcttgactag caaaaaaagt ataacgattt
    20341 ctagcttggc tttctcctgc aaaagagata agcaggtttt gttctgtttt tgttccttta
    20401 attgatttca tggtaatttt tttgggtgtt aaacgttctt tttaatatgc tgtgtttttt
    20461 ctaattgcat ggcttctctg tgtggcacgc ccaaaaagcg gcacgttact tgcgtaggtt
    20521 gcactttcca cacgcgtaca tttttaatcg ctggatctga atattgaaaa tctgttttgg
    20581 ctgtatagtg tgccatgata atgttcatga tacgtatttt ctctgcgtca tcagcaataa
    20641 actcaacttg cccatcgata attaccgatt ttgtttgcat taagtaagta catgctattt
    20701 gctcggcagt ataatacaac gacgcattgg cacaaaaact aatacaaatt tttggatttt
    20761 tttctaatat atctgttttt cttccttgcg gaccagaatg caagtacaaa atcccatctt
    20821 gatagccaaa attcattgga aatacgtagg gcgtagcatc tatatcggca actgcaacaa
    20881 aacaaaattg gcatgaacga ataacttcgt caatttttgg ttgttcggtt atcgttactg
    20941 ttttcatcta ctgagctact tttttgtttt tattaacatt tcagaaacac ctgcgaagat
    21001 acagatatat tactaaactt acaactactt gatgcatttt taacacacag aaaagcaagt
    21061 ttgttggtga tgaaaaaata aatgattgat agtagaaatt attgtatgcc tttggattgt
    21121 atgagtgggg ctcaacaaag acgtttaaac ttgtacaccg cataatttac acaaatatat
    21181 cttgtgccct attggtagga ttatttttaa tataatccga gatgctgaga tacgatagtt
    21241 cgttacggat gatgtgttcc caataattgc gttgccacat tcttttatcg aatgtgtgcc
    21301 aatagttgtt ttttacacca cggattcctt tcccctctac aaaaaaagcg aataacccat
    21361 ataggttact cgctttattt attcgtcttt agcaggcgat ataatcgtgc gg
//
LOCUS       DAOSRP010000015        21387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2655_length_21387_cov_5.214748, whole genome shotgun sequence.
ACCESSION   DAOSRP010000015 DAOSRP010000000
VERSION     DAOSRP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_21387_cov_5.214748"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLJ82_01880"
     CDS             complement(<1..230)
                     /locus_tag="PLJ82_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR40292.1"
                     /translation="MNLITHVGRYSQLMTGVFKKPDKWNITLRQIVKEINKLGIDSLG
                     ITIIISVFIGSVITLQMVLNTANPLLPTYVTG"
     gene            491..1798
                     /locus_tag="PLJ82_01885"
     CDS             491..1798
                     /locus_tag="PLJ82_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOR40293.1"
                     /translation="MIVLLFGTHLYLTVRTGFVQRKIGTAIKLSFTSDKESAGDISQF
                     GSLAIALAATIGTGNIIGIATAIALGGPGAVFWCWLTGVFGIATKYSESLLAVKYRVK
                     TESGTMIGGAMVVLDKVLKMRWLAVLFCVFTAIAAFGIGNMTQANSISVLVEQHFAIP
                     PYVTGIILAVSVGIVMFGGVKWVATVCKALVPTMALVYIIGCLLLLGLNYQYVGSAIS
                     LIISSAFSSDAVGGGFTGAAVMMAMRFGIARGLFSNESGLGSAPIVAAAAQTKNAVRQ
                     ALISATGTFWDTVVICALTGIVLVSSVLAFPEIDFHHGPMLTSMAFSKLHVWGSSILT
                     FGLLTFVFSTLLGWSYYAERTLEYLFGKKVIVYYRVAWVFAIFFGAVLKISLVWNISD
                     AANALMAIPNLIMVLMLSGVIAKETRKYLWDNNLDTVDTEMVR"
     gene            2048..2635
                     /gene="ruvA"
                     /locus_tag="PLJ82_01890"
     CDS             2048..2635
                     /gene="ruvA"
                     /locus_tag="PLJ82_01890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOR40294.1"
                     /translation="MIEYIKGDVVELNPSFVVIETNGIGYFIHISLTTYAHLQQTTAC
                     QLYVYESIREDAHVLYGFSKKEERQIFLHLISVSGVGANTARVILSSLSALEIQEAIA
                     TANENVLKAVKGIGLKTAQRIIVDLKDKLGNMDLATKNVVTLQVNTSMKKEAVSALVM
                     LGFPASASQKVVDKILADNADMKIEDAIKKALKML"
     gene            2656..10068
                     /gene="sprA"
                     /locus_tag="PLJ82_01895"
     CDS             2656..10068
                     /gene="sprA"
                     /locus_tag="PLJ82_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOR40295.1"
                     /translation="MTKIHKILVFISLCGLIFYSSLVANDTIDSSVSIAKKETEPSDE
                     SLRYPISQSLENAPLTQQTNAVIDLPSPQNIASSIEYDPLTEQFIFSKKLNDQDLITP
                     TYLTPEEYSKYQMQQSVNAYWRTRNRQEKDNEKPFSLTDIQIGLGDADKIFGPGGVQL
                     KLQGSAELLFGLKFYRSKNPSLSERLQSPPPVFDFDQKIQLNVNGSVGDKMSFGLNYN
                     TEATFDFDQSMLKLRYEGKEDDIIRKIEAGNVSMPLSGSLITGNTTLFGFKTEMQFGK
                     LNVTTVLSQQESESKTINLSGGAQTTDFDIAADQYDENRHFFLAHYFRDNYDSFMSKL
                     PHITSGIVINKIEVWITNKNAVDFNQARNIVGFMDLAEPTRINNTHWIPSATTLVPDN
                     DANSLYREVIGIDGYRTISLVNNALGQSLSGFGIVGGEDYVKIESARRLEPTEYSLNK
                     QLGFISLRRTLGTDEVLAVAYEYTYNGKVYQVGEFSTDGIESPNVLTLKLLKGVSQSP
                     NVPMWDLMMKNVYSLGAFQVQKDKFRLDVMYRSDSTGVDLNYIPKGKIANTILLRVMN
                     LDRLDSRSQQNPDGVFDFVENFTVIPENGRIIFPVVEPFGSHLRKAFDDDVVANEFVF
                     QELYDSTIVVAKEVAEKNKFRLVGQYRSSSGSEIRLNAMNVPQGSVKVTAGGRQLTEN
                     TDYTVDYMMGVVNILNQDIIESGTPISINLENKSLFSMQRKTLVGTHLDYAFSDKFNV
                     GGTFMHLSEKPLTQKVLMGNEPISNTIWGLNTSYTTQSQWLTNLLDKIPLLNLTEPSS
                     INLNAEFAQMIPGHSKTIDEQGIAYLDDFESTKISFDIRHPQMWGLASTPYHPTGGLF
                     PEAALVNDIEYGKNRALLAWYYIDPIFTRNTSRTPLHIKNDKEQLSNHFVREIREREI
                     FPNRQLLYGQSGYIQTLNVAYYPTERGPYNIYPTSYDSEGKLTNPKGKWGGMMRRLET
                     TDFENNNIEYMEFWLLDPFVYDTLHVMKGGDLYVNLGDISEDILKDGRKFFENGLPVT
                     DAQPQAETTNWGKVPSRQAVVYAFDNEANARKLQDVGLNGLTTNEEFTFGAYQTFINQ
                     LKATLTPEALQKFENDPFSPLNDPAGDNFSHYRSSYYDNQQKGVLERYKRYNGTEGNS
                     PAQADTNEDFTTSATNLPDVEDINNDNTLNEYEKYYQYKVPLRPEEMVVGKNYIADVV
                     TSTVELKNGKTEQVKWYQFKIPIRDAQNYTAIGGIRDFKSIRFMRMFLTDFEEDVMLR
                     FATLQLVKGQWKTYTKELYSLDKTPTSTASISMSAVNIEENADKTPVNYVLPPGVSRV
                     IDPSQQQIRQDNEQALSMRILQLSPGDARAVYKTSGVDMRQYKRMQMFVHAEALADNT
                     TQLVDNEMRVFIRLGSDFRNNYYEYEIPLTLTPPGNYSTNKTSDRETVWPSDNMFDFP
                     LELLTDLKIKRNQEKNRTGSVVSNTTPYYQYDPTKPSNKMTIVGNPNLGEVEVIMIGV
                     RNSSRDVKSAEVWVNELRLTEFNEEGGIAALANLGVNLSDFGRVNVSGRVETVGFGGI
                     EQNVMDRRIDDYYEYNVSSVFDMGRFFPQRARVRLPIAYSYAQQVSSPQYNPLDKDVL
                     LEEALDAAELKTQRDSIRDRSLDVVTNQSISVSNATIGIKSKTPMPYDPANVTVNYAY
                     SETERQNPTTERDIEQNYRASLVYDYSWNPKPVEPFAESKLFAKPAWQLIRDINFYYA
                     PNKISFNTSMVRYYYEQKLRNLTDNSTHNLIPTSFQKEFMWNTNTDIRWDLTRSIKIA
                     FTSGNQARITENDGGVNKKLYPDEYEAWKDSVLTSILQLGNPYSYNQRINASWVLPFS
                     KIPSLNWITSNVKYTGSYNWEYGTVIDDQNLGSTIYNQGAWNVDGRFNFEMLYNKSKY
                     LREINRKYGSSRPQPTPQSTTAPKKFEQIITLKKGEKVVVQHNLNDLKPIVKNLADGK
                     VYSIRYTTIDANSLSFVPQTDGEVSLAFEQRSSQQPDFSTQTLAYSSRFLMMLRNMSF
                     TYNETEGTSIYGFKPGVTLFGLDKYNGRMAPGWDFVFGLQDENFVNRAMANDWLVLSN
                     ALNPAVYKHTQEFQIRALVEPIAGFKINIVADRVEVKDKQVQFSYADMPSTVGGNFSM
                     SYLAIATAFSSIADDGSFTSDVYNQFVQNRALIAARLEEKRQGTLYPDKGFISDASLG
                     NSAYDPTISSYSLNSPDVLIPAFLAAYSGADVRKVSLSPFASFWNLMPNWQVSYDGLS
                     RIAWINDNFRSVNLRHAYSAKYTVGAFTSYLTYVGDSEDNGYIKDVLTGNPIASSQYS
                     ISSVSINELFNPLIGIDLNMKNSLSTKVEIRKGRNLVLNLASNQLVETLNDEIVVGMG
                     YKISDFDVILRLQSNQQKKVKNDLNLRADFSIRDNKTIVRKIEEAYAQATSGNMILSV
                     KFLADYVFSERINISFYYDLQANNPVVMTSYPISNSNVGFSVKMLLTR"
     gene            complement(10073..11152)
                     /locus_tag="PLJ82_01900"
     CDS             complement(10073..11152)
                     /locus_tag="PLJ82_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOR40296.1"
                     /translation="MLKKLDIYIIKKFLGAYFLSIILIMSIAIVIDLTEKLDNFYATN
                     LELSTIIFDYYINFIPYYANLFSSLFTFISVIFVTSKMAFNSEIIAMLASGISLKRIL
                     FPYFIAAGIIALITFFIGGYIIPPGNKVRYEFEQKYIKKKPAEKTIRNIQLKVSTNQI
                     AYIERFELEDNTGYRFSLDTFEGKTLVSRTTSNTIHWDSTYVWTLINYTKRDFVGLKE
                     ELSMGDKTTITINMKPSDFGWIKNQYEHLSNPALKAHIEEQTQRGTAGLNLYKLEYEK
                     RYALPFAAFVLTLIGVSLGSRKVRGGTGLHILIGFMLSITYVLSTMISSSLTVKAGFN
                     PMVAIWSPNVVYFLIGLYLYKIAPK"
     gene            11319..11405
                     /locus_tag="PLJ82_01905"
                     /pseudo
     CDS             11319..11405
                     /locus_tag="PLJ82_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
     gene            11484..12281
                     /locus_tag="PLJ82_01910"
     CDS             11484..12281
                     /locus_tag="PLJ82_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOR40297.1"
                     /translation="MRKYFLLVSVVAVLFSCSTVKKQATGESNAILPDVHTSRVALDW
                     QGVYRGMLPCASCEGIKTQLELFDSTYVLSTQYSGKGDSIYVSNGRFIWSQSGNSLVL
                     IDTLFTDSLHYKIAENQVIQLDKDGNEIKSGLRNMYVLQKDTEQLVDKMWQLTALQGK
                     QLTANYSVFPQPYIIFYADNKVGGSGGCNRFFGNYMLSAGSHIVFSAIGSTRRACLES
                     MEVENEFLSIFHQIDTYSLKQDTLHLLSKEGDILATFHYHYFSDLMQ"
     gene            12287..12592
                     /locus_tag="PLJ82_01915"
     CDS             12287..12592
                     /locus_tag="PLJ82_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40298.1"
                     /translation="MKKISLLFILNLFSVVAFSQFTLGPKVGYLTQTLSVKQEDIKAS
                     LQDKAIFGLFMRIGNSVYVQPEINYFTSGAVFKRPQQGSVNPIEQEVYLQNVQIPFL"
     gene            12634..12909
                     /locus_tag="PLJ82_01920"
     CDS             12634..12909
                     /locus_tag="PLJ82_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40299.1"
                     /translation="MMGPTANIVVEKRINTQESGTYINPIKEADIKDVYWGLQVGGGM
                     DIASVTLDVQYIIGLSSIIGDVEIEGNPVLFDSKNQGFVVTLGLKLF"
     gene            complement(12945..13397)
                     /locus_tag="PLJ82_01925"
     CDS             complement(12945..13397)
                     /locus_tag="PLJ82_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR40300.1"
                     /translation="MSYEQLKLSNQLCFPMYAASRLITREYQPFLDTLGITYPQYLVL
                     MVLWEHGEKSVNEIANTLLLNTNTITPLLKRMEKIGLIERERSLQDERKILVCVSGKG
                     KELEEKAASIPMQLIQNLGKSKLSINDLMTLRNQLNDLIDFLQNKESK"
     gene            complement(13430..13918)
                     /locus_tag="PLJ82_01930"
     CDS             complement(13430..13918)
                     /locus_tag="PLJ82_01930"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOR40301.1"
                     /translation="MASQLAFYDFTAKSLQGKETSLATYAGKTIIIVNTASKCGLTPQ
                     FEGLENLYQEYKDKGLVILGFPCNQFANQEPGDEKSISEGCLLNYGVTFPMFAKVDVN
                     GSHAHPIYVYLKKELPGFLGVKGIKWNFTKFVIDKNGNPVKRFSPYDTPEKMKAYIEK
                     IL"
     gene            complement(14396..14848)
                     /gene="tnpA"
                     /locus_tag="PLJ82_01935"
     CDS             complement(14396..14848)
                     /gene="tnpA"
                     /locus_tag="PLJ82_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOR40302.1"
                     /translation="MSQSLSKLYTHIVFHTKHNQDLIKADVENELFAYLGGILKSNKC
                     VPIIINGTENHLHILCIQSKNIALADLLEELKRKSSRWLKTKGAHYTDFAWQGGYGAF
                     SVSQGKVDVVKRYIENQKEHHKTETIKDEYIRFLTEYEIDYNKDYLWN"
     gene            complement(15234..15306)
                     /locus_tag="PLJ82_01940"
     tRNA            complement(15234..15306)
                     /locus_tag="PLJ82_01940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15271..15273),aa:Met,seq:cat)
     gene            15556..16413
                     /locus_tag="PLJ82_01945"
     CDS             15556..16413
                     /locus_tag="PLJ82_01945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR40303.1"
                     /translation="MKKKIGNFFKKIRIKYKLSVLNENTLEEEYRFRLSALSLFTLFM
                     GLTLITFTLFSILILTTPLKRLLPDNTDRKVRNEVVLSALRVDSLADELNKSQQYLAV
                     IREIVAGDVPIDTTHSTESLISSRIDEKFIEKSDAEKAFAEQYEQEEKYNIINPLTQQ
                     QNTNLVFFTPVKGIIREPFQPRINNHFGISIVVNQSKSVSSIYKGTVILTGYDVNNGY
                     FIQVLHPQNFISIYKQATVILKKEGDLVKTGEVIALVDAQGNKPPVLQFELWYEGRAQ
                     DPQDYIIFE"
     gene            16429..17580
                     /locus_tag="PLJ82_01950"
     CDS             16429..17580
                     /locus_tag="PLJ82_01950"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOR40304.1"
                     /translation="MNKKQIAILGSTGSIGTQALEVIAAHPDKFEVYAITANNSVDLL
                     IEQAVRFQPEMVIIANEQHYAYIKKALENYPIKVYAGVKAICEMVELPSVDVVLTAMV
                     GYAGLLPTIHAVKAGKRIALANKETLVVAGEIICDLAQQYRSAIIPVDSEHSAIFQCL
                     IGEQNASVEKIILTASGGPFRQKTYDELRQVTTRDALKHPNWEMGAKITIDSASLMNK
                     GFEVIEAKWLFGLKPSQIEVLIHPQSIIHSMVQFVDGSVKAQIGLPDMKLPIQYAFAF
                     PQRIENNYPRLDFTQYARFDFQQPDTSKFRNLAFAYDAMEKGGTMPCVLNAANEVVVA
                     AFLEEKIGFLHMSDIIEKTMQTASWIAKPTYEDYVLADKQARELTLAQF"
     gene            17587..18906
                     /gene="rseP"
                     /locus_tag="PLJ82_01955"
     CDS             17587..18906
                     /gene="rseP"
                     /locus_tag="PLJ82_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOR40305.1"
                     /translation="MDILIKVVQLLASLSILVLIHEMGHFLAAKLFKVRVEKFYLFFN
                     PWFTPIKFKPKNSETEYGIGWLPLGGYVKLAGMVDESMDTNQLKQPVQDWEFRSKPAW
                     QRLIIMAAGVIMNFVLAIAIYAATLFYYGEEYVPLHKVSLGMSFSEVAKKQGFQDGDI
                     LIAADGKTLKRLDEDNFRKILNAKQVLVNRAGDLTMVQLTEDFPQQLIASNEGFTSYR
                     IPFVVREVMPETPAFGAGLQKGDSVVSVNDDVLCTQDEFSNVFLANKNTQIMIGFYRQ
                     GDFLSTYITPDSAGMIGVYLTPFYEVYPTTKVTYGVLKSIPAGIKKGVKKFTGYASDM
                     KHLMSKQGAESIGGFGSITQLFPPFFSFEIFLEITAYLSVILAFMNILPIPALDGGHI
                     AFLTYEVITRRKVSQEVMMTAQKIGLLLLLFLLFYANGMDVVRAFFR"
     gene            complement(18920..19552)
                     /locus_tag="PLJ82_01960"
     CDS             complement(18920..19552)
                     /locus_tag="PLJ82_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOR40306.1"
                     /translation="MEDIVVYVEPSNNLANTLLLVEKTTPYNINQINWENFPYKPTCK
                     VHIGWNEDYIYLLFKVEEKHTLAQETALHGNVSQDSCVEFFVSFDNRNTYYNFEFNAI
                     GSMHVCCRNCDGTNKIAFTDKQLAAIETLTSFEKFTALNVYNTHWQLGIAIPAPLFNK
                     SDWYKNTIWANFYKCGDKLQEPHYLSWKSIVSPKPQFHVPHFFGSLRCVK"
     gene            complement(19571..20920)
                     /locus_tag="PLJ82_01965"
     CDS             complement(19571..20920)
                     /locus_tag="PLJ82_01965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR40307.1"
                     /translation="MNTFDQLELTTPLRNAIADMGLIEPTQIQTEAFSVIRSGKDMVG
                     IAQTGTGKTLAYLLPILRDLGFSKQANPRILIMVPTRELVVQVVDTIKQITAYMNVRV
                     LGVYGGVNMATQQQAALQGMDIVVATPGRLYDLVMIRAIQLKSIAKLVIDEVDVMLDL
                     GFRPQLTNLFELMPQKRQNIMFSATMTEEVDALLDDFFTIPERITVAISGTPLDNIEQ
                     TCYPVKNFYTKANLLAYLLDNRNVFTKVLVFVSTKVAADRLFKELEEEFMDEIGVIHA
                     NKSQNYRLRSLEQFDNGEFRIMIASDVMARGLDLDKLSHVISFDTPKYPENYIHRIGR
                     TGRAKAKGKSILFYSPTEIVYKEAIENLMGITIMEKSFPSKVQLSKRLTPDEQPKEEN
                     SIAQKRKPIEEKNPAFHEKKEKNKKVNLGGSYRRELAKKYKKPKTKGDKIANRKKKK"
     gene            complement(21091..>21387)
                     /locus_tag="PLJ82_01970"
     CDS             complement(21091..>21387)
                     /locus_tag="PLJ82_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOR40308.1"
                     /translation="QFVKGKRQNELSEKHLDNIVETYKFRNERERYSRRVSLDEIKKN
                     DYNLNISRYVSTSEDEIQIDLEDVNIKLTEINKSIKVNTEKHNEYLKELGLKPI"
BASE COUNT         6556 a         3500 c         4203 g         7128 t
ORIGIN      
        1 aaaccagtta cgtaagtagg tagcaatggg ttagctgtat ttaaaaccat ttgtaaggta
       61 ataaccgaac caataaacac cgatatgata atggtgatgc ctaacgaatc aatacctaat
      121 ttgtttattt cttttactat ttgtcggagg gtaatattcc atttatctgg ttttttaaaa
      181 actcctgtca ttaattgaga atacctacca acgtgtgtaa tcagattcat agattttgct
      241 taaaaatagt tcaacccaca aaggtaatta tttttttatt cttattttgt taatatttat
      301 tatacttttg tcgcaacaag caatacgcca ttagtgtatt gtttgcagta tcgtcttttt
      361 attttttatt aactgggtgt aggtctatta taagtaattg ataatcaaat aaataaactt
      421 catagaacaa gtcttaattg ttgtagaaaa attctttgaa gatgcaagtt cttttttgtg
      481 gggtatccct atgattgtgt tgttgtttgg tacacacttg tatcttactg ttcgtacagg
      541 ttttgtacaa cgaaagatag gtactgctat taaattatcg tttacttctg ataaagaatc
      601 agcaggagat atcagtcagt ttggttcgct ggcaatagcc ctagcagcta ctattggcac
      661 aggcaatatt attggcattg ctacagccat tgccttaggt ggtccaggtg ctgttttttg
      721 gtgttggcta acaggtgttt ttggtattgc taccaaatac tcggagtcgc tattagctgt
      781 aaaatatcgg gttaaaaccg aatcgggaac aatgataggt ggagctatgg tagttttaga
      841 taaggtttta aaaatgagat ggttggctgt gttattttgt gtttttacag ccatagctgc
      901 ttttggtatt ggtaacatga cacaagcaaa ttcaatctcg gtattggtag aacagcattt
      961 tgctatacct ccgtatgtta caggtattat attggcagta agtgttggga tagtgatgtt
     1021 tggtggtgta aaatgggtgg caactgtgtg caaagcatta gtacctacga tggctttggt
     1081 atatattatt ggatgtttgt tactccttgg tttaaattat cagtatgtag gttcagcaat
     1141 ttcattaatc atatcctcgg ctttttcttc ggatgccgtt ggaggcggat ttactggagc
     1201 agcagtaatg atggcaatgc gttttggtat tgctcgtgga ttattttcta atgaatcggg
     1261 attgggttcg gcacctatag ttgcagcagc agctcaaaca aaaaatgctg ttcgtcaagc
     1321 tcttatctca gctactggta cattttggga tacagtggtt atttgtgctc ttacagggat
     1381 tgtactagta agtagtgtat tggcttttcc agaaattgat tttcaccatg gaccaatgct
     1441 tacaagcatg gcatttagta aattgcatgt atggggatcg tctattttaa cgtttggatt
     1501 attaaccttt gtgttctcta ccttgttggg gtggtcgtat tatgcagagc gaacactcga
     1561 atatttattt ggtaaaaaag ttattgttta ttaccgtgtt gcgtgggttt ttgctatctt
     1621 ttttggagca gtactcaaaa tttcgttggt gtggaatatt tcagatgcag ccaatgcgtt
     1681 gatggcaata cccaatttaa taatggtatt aatgcttagt ggtgtaatag ctaaagaaac
     1741 gcgtaaatat ttgtgggata ataatttaga tacagtggat accgagatgg ttaggtagct
     1801 aagtattgta acatgcatag ctttagtgtg catatacata acagttttta ctaatcattg
     1861 ttggtatctg ccttttttat acgttaaaaa agattatttt atatacgttt tttgcctttt
     1921 cttcgttcta gctacacacg ttttatataa tgtataagat agcatgtgat ggaaaatttt
     1981 gaaaagacta ttttttttat gtatctttga acccttttta gagccatcca atattagata
     2041 agtatttatg attgaatata ttaagggaga tgttgtagag ctaaatccca gttttgtagt
     2101 aatagaaacc aatggaatag ggtattttat ccatatttca cttactacct atgcccattt
     2161 acaacaaacg acagcttgtc aattgtatgt ttacgaatcc attcgcgaag atgcacatgt
     2221 gttgtatggg ttctcaaaaa aagaagaaag acaaatcttt ttacatctta tctctgtgtc
     2281 gggagtagga gccaatactg ctcgggttat tttatcatct ctttcggcat tagaaataca
     2341 agaagccatt gcaacagcca atgaaaatgt gttgaaggct gtaaaaggaa ttgggttaaa
     2401 aacggcacaa cgtatcattg ttgatttgaa agataaacta ggcaatatgg atttagctac
     2461 taaaaatgta gttactctgc aagttaatac ctcaatgaaa aaagaggcgg tatctgcatt
     2521 ggtgatgtta ggttttccag cctctgcttc acaaaaagtg gtggataaaa ttttggcaga
     2581 taacgcagat atgaaaattg aagacgctat caaaaaagca ttaaaaatgt tgtaacaata
     2641 aagcgttcgt ttaccttgac taagatacat aaaatactcg tttttataag cctttgtggg
     2701 ttgattttct actcttcgtt ggtggctaac gatactattg actcctctgt aagtatagca
     2761 aaaaaagaaa cagaaccatc tgatgaatcg ttacgttatc ctatttcgca atcgttggaa
     2821 aatgcccctt taacccaaca gacgaatgcc gtaatagact taccatctcc acaaaatata
     2881 gcatcttcta tcgaatatga cccgttgact gaacagttta ttttttctaa gaaactgaac
     2941 gatcaagatt taataacgcc aacatatcta acgccagaag agtactctaa gtaccaaatg
     3001 caacaatctg ttaatgccta ttggcgtaca cgcaatagac aggaaaaaga caatgaaaaa
     3061 ccattttctc ttaccgatat tcaaattggg ttgggcgatg cggacaaaat atttggacca
     3121 ggaggggtgc agttgaagct tcaaggttcg gcagaattat tgttcgggct taaattctat
     3181 aggtctaaaa atccatcttt aagtgaacga ttacagagtc cacctcctgt atttgatttt
     3241 gatcaaaaaa tacaactgaa tgtgaatggt tctgttggag acaaaatgtc cttcggatta
     3301 aattacaata ctgaagccac ttttgatttc gaccaatcaa tgctcaaact gcgttacgaa
     3361 ggcaaagaag acgatattat acgcaaaatt gaagcaggta acgtaagtat gccattgagt
     3421 ggaagtttga ttactggtaa tactacttta tttgggttta aaacggaaat gcaatttggt
     3481 aagttaaatg taaccaccgt tttatcgcaa caagaatcag aatcaaaaac tattaattta
     3541 agcggaggag cacagacgac agattttgat attgccgctg atcagtatga tgaaaatagg
     3601 cattttttcc ttgcacacta ttttcgcgat aattatgatt cttttatgag caaattgcca
     3661 catatcacct ctggtatagt gattaataaa attgaagtgt ggattaccaa caaaaatgca
     3721 gttgatttta atcaggctcg taatattgtt ggttttatgg atttagctga accaacacgc
     3781 atcaataata ctcattggat accttctgct accactttag ttcctgataa tgatgctaat
     3841 agtttgtatc gtgaagtgat aggtattgat ggatatcgta ctatttcttt ggtaaataat
     3901 gcactgggac aaagtttgtc aggttttggt attgtgggag gagaggatta cgtaaaaatt
     3961 gaaagtgctc gacgattaga accaacggaa tattcattaa acaaacaatt agggtttatt
     4021 tcgttgcgac gcacattagg cacagatgag gtattggctg tagcatacga atatacatac
     4081 aatggaaaag tataccaagt aggggaattt tctactgatg gtattgagtc gcctaatgtg
     4141 ttaacactga aacttctaaa aggtgtttcc caatcgccaa acgtacctat gtgggatttg
     4201 atgatgaaga atgtatattc gcttggagca tttcaagtac aaaaagacaa attccgtttg
     4261 gatgtaatgt accgtagtga ttctactgga gtggatttaa actatatccc caaaggaaaa
     4321 atagccaata caattttatt gagggtgatg aatttagacc gattagactc tcgctctcaa
     4381 caaaatccag acggagtgtt tgattttgtt gaaaatttta ctgttattcc cgaaaatggt
     4441 cgtattatat ttccggttgt tgagcctttt ggctctcatt tgcgtaaagc ttttgatgat
     4501 gatgtggtag caaacgaatt tgtgtttcag gagttgtacg attctactat tgttgtagcc
     4561 aaagaagtgg cagaaaaaaa taaatttcgc ttggtggggc aataccgctc ttcttcggga
     4621 tcggaaattc ggttaaatgc aatgaatgtg cctcaaggat cagtaaaggt aactgctggt
     4681 gggcgtcagc taacagaaaa tactgattat acagtagatt atatgatggg ggttgtcaat
     4741 attttgaatc aagatattat tgagtcaggc acaccaataa gtattaattt ggaaaataaa
     4801 tcgctcttta gtatgcaacg taagaccttg gtgggaaccc atttggatta tgcatttagc
     4861 gataaattta atgtaggagg tacttttatg catttgtcag aaaaacctct tacccaaaag
     4921 gtcctcatgg gaaatgaacc tatttcaaat acaatctggg ggctaaatac ttcttatact
     4981 acccaatccc aatggttaac taatctgtta gataaaattc ctttgctcaa tttaacagag
     5041 ccatcttcta tcaatttaaa tgcagagttt gctcaaatga ttcctggaca ttccaaaaca
     5101 atagatgaac aaggaattgc ttatttagat gattttgaat ctaccaaaat atcgtttgat
     5161 atccgtcatc ctcaaatgtg ggggctagct agtactcctt atcatccaac aggtggttta
     5221 tttccagagg cagccttggt aaatgatatt gagtatggca aaaatagagc cttgcttgcc
     5281 tggtattaca tcgacccaat ttttacacgc aatacttctc gaacgccatt gcatattaaa
     5341 aatgacaaag aacagctatc aaatcatttt gtgcgtgaaa tcagagaacg tgaaattttc
     5401 cctaatcgac aattgttgta tggacaatcg gggtatattc aaacattaaa tgtagcgtat
     5461 tatcctacag agcgtggacc gtataatatt tatccaacta gttatgactc agaaggaaag
     5521 ttaactaacc caaaagggaa atggggtggt atgatgagac ggttagaaac aaccgatttt
     5581 gaaaacaaca atatcgaata catggagttt tggttgctcg atccctttgt gtacgatact
     5641 ttacatgtga tgaaaggtgg agatttatat gtcaatttag gagatatttc ggaagatatt
     5701 ttaaaagatg gcagaaagtt ttttgaaaat ggcttacccg taactgatgc tcagccacaa
     5761 gctgaaacaa ccaattgggg aaaagtgcca tcacgtcaag cggtagtgta tgcatttgat
     5821 aatgaggcta atgcacgaaa attgcaagat gttggattga atggattaac taccaatgaa
     5881 gagtttactt ttggggctta ccaaactttt attaatcaat tgaaggcaac attaacgcca
     5941 gaagccttac agaaattcga aaacgatcca ttttctcccc tcaacgatcc agcaggggat
     6001 aatttttcgc attatagaag ctcgtattac gataaccaac aaaaaggagt attagaacgc
     6061 tacaaacgct ataacggaac tgaaggtaat tctcctgcac aggctgatac aaacgaagac
     6121 tttaccacgt cggctaccaa tttgcctgat gtagaagata ttaataatga taatacgctc
     6181 aacgaatacg aaaaatatta tcaatataag gtgccgttac gtcccgaaga aatggtggta
     6241 gggaaaaatt atattgcaga tgttgtaact tctaccgtag agctaaaaaa tggcaaaaca
     6301 gagcaagtaa aatggtatca atttaagatt ccaattcgtg atgcacagaa ctatacggct
     6361 attggtggta ttcgcgattt taagtctatt cgttttatgc gtatgttttt aaccgacttt
     6421 gaagaagatg taatgcttcg ctttgctacg ttacaattgg taaaaggaca gtggaaaaca
     6481 tacacaaaag aattatattc cttagataaa acacctactt ccacagcctc catatctatg
     6541 tctgctgtca atattgaaga aaatgcagat aaaactcctg tcaattatgt attaccccct
     6601 ggagttagtc gtgttattga ccctagccag cagcaaattc gtcaggataa cgaacaagcc
     6661 ttatccatgc gtattttgca attatcgcct ggcgatgcac gtgccgtata caaaacatcg
     6721 ggcgtagata tgcgtcagta caaacgaatg cagatgttcg ttcacgctga ggctttggct
     6781 gataacacaa cgcaattagt tgataacgaa atgcgtgtat ttattcgttt gggatctgat
     6841 tttagaaata attactacga atatgagatt cctcttacat taactcctcc aggtaattat
     6901 tcaaccaata aaacttcgga tagagaaaca gtatggccct ctgataatat gtttgatttt
     6961 cctcttgaat tacttactga tttaaaaatt aaacgaaatc aagaaaaaaa ccgaactgga
     7021 tctgttgttt ctaatacgac gccatattat cagtacgacc ctactaagcc aagcaataaa
     7081 atgactattg taggaaatcc aaatttggga gaagtagagg ttattatgat tggggtacga
     7141 aattcctctc gcgatgtaaa atctgcggaa gtatgggtaa atgagttgcg tttaacggaa
     7201 tttaacgaag aaggaggtat tgcagcttta gctaatttag gtgttaatct gtctgatttt
     7261 ggtagagtaa atgtgtctgg tagggtagaa actgttggat ttggtggcat agagcaaaat
     7321 gtaatggatc gtcgtattga cgattattac gaatacaatg tatcatccgt atttgatatg
     7381 ggtagatttt ttcctcaacg tgctcgtgta cgtttgccta tcgcttattc gtacgctcag
     7441 caagtatcta gtccacaata caatcctctt gataaagatg tacttttaga agaagcttta
     7501 gacgctgctg aacttaaaac gcaacgcgac tctattcgcg atagatcatt ggatgttgtt
     7561 accaatcaaa gtatatctgt gtccaatgca acgattggga taaaaagcaa aacgcctatg
     7621 ccttacgacc ctgctaatgt tactgttaat tatgcttatt ctgaaacaga aagacaaaat
     7681 cctacgacag aaagagatat agagcaaaat taccgtgctt cattggtgta tgactatagt
     7741 tggaatccaa aacctgtaga gccttttgcc gaaagtaaac tgttcgcgaa acctgcatgg
     7801 caattgatcc gtgatatcaa tttttattat gctcctaaca aaatttcttt taatacgagt
     7861 atggtacgct attattacga acaaaaatta cgaaatttaa cggataatag tactcataat
     7921 ttaataccta cttcgtttca aaaagaattt atgtggaata caaataccga tattcgatgg
     7981 gatttgactc gttctatcaa gatagcattt acgtcaggca atcaggctcg cattactgaa
     8041 aatgatggtg gtgtaaataa aaaattatat ccagatgaat acgaggcttg gaaagattct
     8101 gtgcttacaa gtattttgca attaggcaat ccctattcat acaaccaacg aattaatgct
     8161 tcttgggtac tgcctttcag taaaattcct agtttgaatt ggattacgag caatgtgaaa
     8221 tatacaggaa gttataattg ggaatacggt acagtaattg atgaccaaaa cttaggaagt
     8281 acaatttaca atcaaggtgc ttggaatgtg gatggacggt ttaattttga aatgctgtat
     8341 aataaatcta aatatctgcg cgaaatcaat agaaaatatg gtagtagtcg tcctcaacca
     8401 actccacaat caactactgc ccctaagaag tttgaacaga ttataaccct aaagaaagga
     8461 gaaaaagttg ttgttcagca taatcttaac gacctaaaac ctattgttaa gaatcttgca
     8521 gatggaaaag tatattccat tcgttacact acaattgatg ctaattctct ttcgtttgtg
     8581 ccacaaactg atggagaggt ttcgttggct tttgaacaac gttcttctca gcaacctgat
     8641 ttttcgaccc aaacgttggc ttattcttct cgctttttaa tgatgttgcg aaatatgtca
     8701 tttacgtata atgaaacaga aggaacttct atttacgggt ttaaaccagg tgtaacacta
     8761 tttggtttag ataagtataa tggtagaatg gctcctggat gggattttgt gtttggtttg
     8821 caagacgaaa attttgtaaa cagagctatg gcaaatgact ggttagtatt gagtaatgca
     8881 ttaaatccag cggtgtacaa acatacacaa gaatttcaga taagggcgtt agttgaacct
     8941 attgcaggtt tcaaaataaa tattgtagca gatcgcgttg aagtaaaaga taaacaagtg
     9001 caattttcgt atgcagatat gccatcaacg gtgggtggaa acttttctat gagctatctt
     9061 gctattgcca cagcatttag ttctattgct gatgatggtt cttttacttc agatgtgtat
     9121 aatcaatttg tgcagaatag ggctttgata gctgcaaggt tagaagaaaa acgacaggga
     9181 acgctctatc ccgacaaagg atttatatct gatgcaagtt tgggaaatag tgcctatgat
     9241 ccaactatca gttcttattc tctcaattca cctgatgtgc tcatccctgc ttttttagcc
     9301 gcttattcgg gtgctgatgt gcgaaaagta tcgttaagtc cttttgcttc cttttggaac
     9361 ttaatgccta attggcaagt ttcttatgat ggattatctc gtattgcatg gataaatgat
     9421 aatttccgta gtgtgaattt gcgacatgcc tatagtgcaa aatatacggt tggagcattt
     9481 acatcctatt taacctatgt gggagatagt gaagacaatg gctatattaa agatgtgtta
     9541 actggtaatc ctattgcctc gtcacaatac agcataagta gtgtgtctat caacgaactt
     9601 tttaatccat taattggtat tgacctcaac atgaaaaaca gtttatcaac caaagtagaa
     9661 atacgtaaag gaagaaatct tgttctgaat ttagcaagta accagctagt ggaaacattg
     9721 aatgatgaaa ttgtggtagg aatgggatat aaaattagtg attttgatgt gatattacgt
     9781 ttgcaatcaa atcaacaaaa aaaggtgaaa aatgacctca atttacgtgc agatttctct
     9841 attcgagata ataaaactat tgtacgaaaa attgaagaag catatgctca ggctactagc
     9901 ggcaacatga ttttgagcgt taagtttttg gcagactatg tatttagtga gcgtataaat
     9961 atcagttttt actacgattt acaggctaat aatcctgtgg ttatgacatc atatcctatc
    10021 tccaattcca atgtaggatt tagtgtcaag atgcttctaa ctagataaaa aattactttg
    10081 gggctatttt ataaaggtat aatccaatta aaaaatagac aacattgggc gaccatatgg
    10141 ctaccattgg attaaatcct gcttttactg ttaatgatga cgatatcatt gttgataata
    10201 catacgttat acttaacata aacccaatta aaatgtgtaa gcctgtacct cctctaactt
    10261 tgcgagaccc caatgataca ccaattagag ttaatacaaa agctgcaaat ggtagggcat
    10321 aacgtttttc gtattcaagc ttatacaggt tgagacctgc tgttcctcgt tgtgtttgtt
    10381 cttcaatatg tgcttttaac gcaggattgc taagatgttc gtattgattt tttatccatc
    10441 caaaatcaga tggtttcata ttaattgtaa ttgttgtttt atctcccata gacaattctt
    10501 cctttaatcc aacaaaatca cgcttagtgt aatttatcag cgtccataca tatgttgagt
    10561 cccaatggat ggtgttagaa gttgtgcgtg atactaatgt ttttccttca aaagtgtcta
    10621 acgaaaacct gtatcctgtg ttatcttcta attcaaaacg ttcaatatag gcaatttggt
    10681 ttgtggacac ttttaactgg atgtttcgga tggttttttc agctggtttt tttttaatat
    10741 atttctgttc aaattcgtaa cgcactttgt taccaggagg aataatatag cctccgataa
    10801 agaaagtaat cagtgcgata atgcctgctg caataaaata agggaataaa atacgtttca
    10861 agcttatacc gcttgcaagc atagcaataa tttctgaatt gaaagccatt ttggaagtaa
    10921 caaatataac tgaaataaat gtaaataagg aactaaataa attggcgtaa taaggaataa
    10981 agttaatgta gtagtcaaaa ataatagtag ataattcaag atttgtagca taaaaattgt
    11041 ctaatttttc ggttaaatca atgacaatgg cgatgctcat tataagtatg atagatagga
    11101 aataggctcc caaaaatttt ttaataatat atatatcaag tttttttaac atggctatgg
    11161 ttttataaat taaattgcgg tgcaaaaata cgaaaaatta actgaaatag atagaatgct
    11221 tatttttaat aaaaaaaatg agaatatttc gtttattgca aagtttgttg tatttttgaa
    11281 actaattaaa taagtatgaa tttttaaaag aaacatgtat gaaaacattc gtatgttcag
    11341 tatgtgggta tgttcaccaa ggcgatacac ccccagaaaa gtgtcctaaa tgcaagcaac
    11401 cagcaaaagt atttaagcaa gtgaaataat ttttttaaaa ctatccctaa aaagatagtt
    11461 tttttttata ttactagatc atgatgagaa aatatttttt attagtaagt gtcgttgctg
    11521 tgttattttc ctgttctact gtaaaaaagc aggcaactgg cgaaagtaat gcgattttac
    11581 ccgatgtaca taccagtcgt gttgccctcg attggcaggg agtttatcgt ggtatgttgc
    11641 cttgtgcaag ttgcgagggg ataaaaacac aactcgaact tttcgattct acttatgttc
    11701 tttctacaca atatagtggt aagggtgatt ctatctatgt ttcaaatggt cgttttatat
    11761 ggtctcaaag tggtaattcc ctcgtactta ttgatacact ttttaccgat tcgctgcatt
    11821 ataaaatagc tgaaaatcag gttattcaat tagataaaga tggaaacgag atcaagtcag
    11881 gattacgaaa tatgtatgtg ctacaaaaag atacagaaca attagtggat aaaatgtggc
    11941 aattaacagc ccttcaagga aagcaattaa ctgcgaatta ttcagtattt ccacagccat
    12001 acattatttt ttatgcggac aacaaagtag gtggcagtgg tggatgtaac cgtttttttg
    12061 gtaattatat gctttctgct ggttcacata tagttttttc cgctattgga agtacccgaa
    12121 gagcatgtct tgaatcgatg gaagtggaaa atgagttttt gtccatattt caccaaattg
    12181 acacctatag cctgaagcaa gatacgttac atcttttatc aaaagaagga gatatactag
    12241 ctacctttca ctatcattat tttagtgact taatgcaata agtaagatga aaaagattag
    12301 tttgttgttt attttgaatc ttttctccgt tgtagccttt tcgcagttta cactaggtcc
    12361 aaaggttggt tatcttaccc aaacgctgtc tgttaagcaa gaggatatta aggcaagttt
    12421 acaggataaa gccatttttg gtctttttat gcgtattggt aatagcgtgt atgtgcaacc
    12481 agagataaat tattttacgt caggagctgt tttcaaacga cctcaacaag gttctgtaaa
    12541 tcccattgag caagaagtgt atttgcaaaa tgttcaaatt ccttttttat aggcacaaaa
    12601 ttgattaaca cacgcttgtt taatttacgt gccatgatgg gaccgactgc caatattgtg
    12661 gttgaaaagc gtatcaatac ccaagaatca ggtacgtata tcaatcccat taaagaggca
    12721 gatatcaaag atgtgtattg gggtttgcaa gtaggcggtg gtatggacat tgcatccgtt
    12781 acccttgatg ttcaatacat tattggttta agttctatta ttggtgatgt agaaatagaa
    12841 ggtaatcctg tgttattcga ttctaaaaat cagggatttg tagtaacact tggtctcaaa
    12901 ttgttctaac atagaatagg cttactcaag tgtgaagagt aagtctattt tgattcttta
    12961 ttttgcaaaa aatcaattag gtcgttcagt tgatttctga gggtcatcaa gtcgtttatt
    13021 gacagcttgc ttttacccaa gttttgtatt aattgcatag gaatggaagc tgctttttct
    13081 tctaattctt ttcctttacc gcttacgcaa accaatattt tacgttcatc ttgcaacgac
    13141 cgttctcgct ctatcaagcc gattttttcc attcttttca acaaaggtgt tatggtgttg
    13201 gtgttgagca gtagggtgtt agctatttcg ttcacacttt tttcgccatg ttcccatagc
    13261 accatcagta ccaagtattg tgggtaagta atgcccaatg tatccaaaaa tggttggtat
    13321 tcgcgtgtaa taagccgtga agcagcatac atcggaaagc acaattggtt gctgagtttt
    13381 aattgttcgt agctcatggt aaaaatgttt gtacaaaaat agtatttatt tacaatatct
    13441 tttcaatata agctttcatt ttttcgggtg tatcgtaggg tgaaaatctt tttacaggat
    13501 tcccattttt atcaattaca aacttagtaa aattccattt tatccctttt acgcctaaaa
    13561 agcctggtaa ttcttttttt agatacacat aaattgggtg agcatgtgag ccattcacat
    13621 ctactttggc aaacattgga aatgttacgc catagttgag caagcaacct tcagatatag
    13681 atttttcatc gccaggttct tgattcgcaa attggttgca aggaaagccg agtattacta
    13741 gccccttatc tttgtattct tgatacaagt tttctagccc ttcaaattgg ggtgttagtc
    13801 cacatttgct tgctgtgttt actataataa ttgttttgcc tgcataagtt gcaaggctgg
    13861 tttcttttcc ttgtaatgat ttagctgtaa aatcataaaa tgctagttga cttgccacta
    13921 tcgttattta ttagttgtaa atatatcgta tgcgatacaa atatacggtt gttattttat
    13981 atcgcaagcg atttaaaata atttaacgaa aggaagcaca aaagattttt agaggggggt
    14041 atttatcgga cttgttatat tttgccattc ggtattctta tctctaagcg atgctgttag
    14101 gttgatatta tgttgccctt tcagggcgta gatttgtatg ttttgtgttt ttatcccaag
    14161 gcgatgctgt tgtgttattg ttatattgcc ttttcagggt gttgctatgt tcattggctg
    14221 aaagccataa atatcattaa cctgtggcaa cgccataggt ttaaaatcat ccacccacgc
    14281 tcacgggcat ttttttaacg aagaaaccaa cggtatttcc atcgatttta cgtgccgaat
    14341 aacatctagc tcatatcgca gatggggtcg atatcgttta ccagcttgct accatttaat
    14401 tccaaaggta gtctttatta taatctatct catattctgt taaaaaccga atatattcat
    14461 cttttattgt ttcagtttta tggtgttctt tttgattttc aatataccgc tttacaacat
    14521 ccaccttgcc ttgacttacc gaaaaagctc cgtaaccacc ctgccaagca aaatcagtat
    14581 aatgagctcc ctttgtttta agccaacgac tgctttttcg ttttaattct tcaagcaaat
    14641 ctgctaaagc aatgttcttt gattgaatgc aaaggatatg tagatggttt tcggttccgt
    14701 taataataat cggaacgcat ttatttgatt ttaaaatacc ccctaagtat gcaaataatt
    14761 cgttttcgac atccgctttt attaagtcct gattgtgttt agtatgaaaa actatatgcg
    14821 tgtatagttt tgaaagtgat tgcgacatgt ttttttgttt tatgttgcct tttcagggcg
    14881 ttgctgtatt tcattggttt taggtcatat gtattatcag cttatatgtc tcgtttttct
    14941 tatcccaagg cgatgacatt gggtagatgt tataatgccc tttcagggcg ttgctgtgta
    15001 taattggctg aaagccatat atattcatca gcctatggca acgccatagg tttataatca
    15061 tcccacatca atttcccctg caaagttaaa aagatttcca aatacggatt acatgagagt
    15121 tagaaagtac aaagttagaa agtgtaaaca gtaagagctt gttttttagt cgttgctatc
    15181 caatcagcaa caagcaaaaa agagaagaaa cctgtgtttc ttctcttcca gagttgcggg
    15241 gattcgactc gaacgaatga cctttgggtt atgagcccaa cgagctacca actgctccac
    15301 cccgcgatat attttttagc tataaagctg ttttctccaa ttggactaca aagatagtgc
    15361 gttcatttgt gttatccaaa ttatttggga tatatttttg tttaaaattg ttttgttgga
    15421 aaaaggtatc ctaatttttg ctaaaagttc tctttcatgc gtgttttatg ttttttacat
    15481 gttttatgtt taaaaaaatg ctatttttgt gtggtaaaaa atggagtgtc agtatccata
    15541 tttttgtgta tgccgatgaa aaaaaaaata gggaattttt ttaaaaaaat acggattaag
    15601 tataaacttt ctgttcttaa tgagaataca ttagaggagg agtaccgttt taggttgtct
    15661 gcattgagct tatttaccct ttttatgggg cttacattga taacgtttac cctgttttct
    15721 atccttattc ttactactcc gcttaaacgt ttgttacctg ataataccga tagaaaagtg
    15781 cgtaacgagg ttgtgttgag tgctttgagg gtcgattcgt tggctgatga gcttaataaa
    15841 agccagcaat atttagccgt tatcagagag atagtggcag gcgatgtgcc tattgataca
    15901 acacatagca ctgagtcgtt gatatcaagt aggatagatg aaaaatttat tgaaaaatcg
    15961 gatgccgaaa aagcctttgc tgagcaatac gaacaggaag aaaaatacaa tattattaat
    16021 ccgttgacac aacaacaaaa tacaaatttg gtgtttttta ctcctgtaaa aggcattatt
    16081 agggagccat ttcaacctcg tataaataat cattttggta tttccattgt agtaaatcaa
    16141 agcaaaagtg tctcttctat ttacaagggg acggttatcc tgactggtta tgatgtaaat
    16201 aatggttatt ttatacaggt tttgcatcct caaaatttta tatctatcta caaacaggca
    16261 accgtaattc tgaaaaaaga aggggattta gttaaaacgg gagaagtgat tgcattagtc
    16321 gatgctcaag gcaataaacc tccagtgttg caatttgaat tgtggtatga gggaagggct
    16381 caggatccgc aagattatat catttttgaa taaactgttg acaaccgaat gaataaaaaa
    16441 caaatagcga tacttggttc tactggctct ataggtacgc aagctttaga ggttattgct
    16501 gctcatccag ataagtttga ggtatatgcc attacggcaa ataatagtgt tgatttgcta
    16561 attgagcaag cagttcgttt tcaacccgaa atggtgatta ttgccaatga acagcattat
    16621 gcatatataa aaaaagcctt agaaaattat ccgataaagg tgtatgccgg agtaaaagct
    16681 atttgcgaaa tggtagagtt accgtcggtt gatgtggtac ttacagcgat ggtaggatac
    16741 gcgggcttat tgcctactat tcatgctgta aaagcgggca agcgtatagc tttagccaac
    16801 aaagaaacct tggtggtagc tggcgagatt atctgcgatt tggcacaaca ataccgttcg
    16861 gcaattatcc cagtcgattc ggagcactca gccatttttc aatgtttgat tggcgaacaa
    16921 aatgcttctg tcgaaaaaat aatactgacc gcttcgggtg gtccttttcg ccaaaaaacg
    16981 tacgatgaat tgcgtcaagt aacaacccgt gatgcattga aacatcctaa ttgggagatg
    17041 ggtgcaaaaa ttaccatcga ctctgccagt ttgatgaaca aaggatttga ggtaatagaa
    17101 gctaaatggt tgtttggctt aaagccttcg cagattgagg tattgattca tccgcagtcc
    17161 atcattcatt cgatggttca gtttgttgat ggctcagtga aagcccagat cggtttgcca
    17221 gatatgaaac tacctattca gtatgctttt gcttttccgc aacgcataga aaataactat
    17281 ccacgtttgg attttactca atatgcacgt tttgattttc agcaaccaga cacaagtaag
    17341 tttcgtaatt tggcttttgc ttatgatgcc atggaaaaag gcggaaccat gccttgcgta
    17401 ttgaatgctg ccaacgaagt ggtagtagct gcatttttgg aagaaaaaat aggcttttta
    17461 cacatgagtg atataataga aaaaaccatg caaacagcta gttggattgc aaaaccgact
    17521 tatgaagatt atgtattagc cgataaacaa gctcgggagt taaccttagc acaattttaa
    17581 caaacaatgg atatattaat taaggtagta caattattgg cgagtttatc aattttggta
    17641 ttgatacacg aaatggggca ttttttagct gccaagcttt ttaaagtaag ggtagaaaag
    17701 ttttaccttt ttttcaatcc gtggtttact ccaataaaat ttaaacccaa aaatagtgaa
    17761 acagaatacg gtataggctg gttgcccttg ggtggatatg taaaattagc tggtatggtt
    17821 gatgaatcga tggataccaa tcaattaaaa cagccagtgc aagattggga gtttcgctcc
    17881 aaaccagctt ggcaacgttt aatcattatg gcggctggtg ttatcatgaa tttcgtatta
    17941 gcgatagcta tttatgcagc taccttgttt tattatgggg aggaatatgt gcctttgcat
    18001 aaggtaagtt tgggtatgtc gttttcagaa gttgctaaaa aacaaggttt tcaggatggc
    18061 gatatattga tagcggctga tggcaaaaca ttaaaacgtt tagatgaaga taatttccgt
    18121 aaaatactca atgccaagca agtattggta aatagggctg gcgatttgac tatggtgcaa
    18181 ttgaccgaag attttccaca acaacttatc gcttccaacg aaggatttac ttcgtatcgt
    18241 ataccttttg tggtgagaga agtaatgccc gaaacacccg cttttggtgc tggtttgcaa
    18301 aagggggata gtgtggtgtc agtgaatgat gatgtgcttt gtacacaaga tgagttttca
    18361 aacgtatttc tagccaataa aaatacccaa attatgattg ggttctatcg tcaaggggat
    18421 tttctctcta cgtatattac acctgattct gctggcatga ttggggtgta tcttactcca
    18481 ttttatgagg tgtatccaac taccaaggtt acgtatggtg tgctgaagtc tatccctgcg
    18541 ggaattaaaa aaggggtgaa gaaatttaca ggatatgcta gcgatatgaa gcatctaatg
    18601 agcaagcaag gtgctgaaag tataggtgga tttggttcca ttactcagtt gttccctccg
    18661 tttttttcgt ttgagatatt tttggaaata acggcatatc tgtctgttat attggcgttt
    18721 atgaatatat tacctatacc agctttagat ggtgggcata ttgcattctt aacttacgaa
    18781 gtgataacac gccgtaaagt gagccaagag gtaatgatga cagcacaaaa aattggcttg
    18841 ttgttgttgc tattcttgct gttttatgcc aatggaatgg atgtggttag ggctttcttt
    18901 aggtaaaatg ccttaaatct tatttcacac aacgcaaaga tccaaaaaag tggggtacat
    18961 gaaattgtgg ttttgggctt acgatggatt tccacgatag gtaatgaggt tcttgcaatt
    19021 tatctccaca tttgtaaaaa tttgcccaaa tagtattttt ataccaatca ctcttattga
    19081 acaatggagc aggtatggca atacctaatt gccaatgggt gttatacaca ttgagagctg
    19141 taaatttttc aaaactcgtc agggtttcaa ttgcagctaa ttgtttgtca gtaaatgcaa
    19201 ttttgtttgt gccatcgcag ttgcgacaac atacatgcat agagccaatg gcattgaatt
    19261 caaaattgta gtaggtgtta cggttgtcga aagatacaaa aaattccaca caactatctt
    19321 gcgatacgtt tccatgcagt gcggtttctt gtgccaaggt gtgtttttct tctaccttaa
    19381 acagtaggta tatatagtct tcgttccaac caatatgtac tttgcaagta ggcttgtatg
    19441 ggaaattctc ccaattaatt tgattgatgt tgtacggggt agttttttct actaacaaaa
    19501 gtgtatttgc aagattgtta cttggctcta catatacaac tatatcctcc atagatatca
    19561 atacaattat ttattttttc ttttttctat tggcaatttt atctcccttt gttttgggct
    19621 ttttgtattt ttttgccaac tctctgcggt acgaacctcc taaatttact tttttgttct
    19681 tttctttttt ctcgtgaaag gcaggatttt tttcttctat tggttttcgt ttttgagcaa
    19741 tgctattttc ttctttaggt tgctcgtcgg gagtaagtct ttttgaaagt tgaacttttg
    19801 aaggaaagga tttctccata attgtgattc ccatcaggtt ttcgatagcc tctttgtaaa
    19861 caatttctgt tggactataa aataatattg actttccttt ggcttttgct cgtcctgttc
    19921 gtcctatgcg atggatataa ttttcgggat atttaggtgt gtcaaagctg ataacatgac
    19981 tcagtttatc taaatccaag ccacgagcca ttacatcaga tgctatcata atacgaaatt
    20041 cgccattgtc aaattgctct aatgaacgta aacggtagtt ttggcttttg ttggcatgta
    20101 ttacaccaat ttcgtccata aattcttctt ctaattcttt gaaaagccta tcggcagcga
    20161 cttttgtact gacaaatacc aacactttcg taaacacatt tctattgtcg agtaggtagg
    20221 ctagtaaatt ggctttggta taaaagtttt ttacaggata gcacgtttgt tcgatgttat
    20281 caaggggcgt accactaata gcaacagtaa tgcgttcggg tatggtaaaa aaatcatcta
    20341 acaaagcatc tacttcttcc gtcatagtag ccgaaaacat gatgttttgt cgtttttgag
    20401 gcattagttc aaacaaatta gtgagttgcg gacggaaacc taaatctaac attacatcta
    20461 cttcgtctat cactaattta gctatcgact tgagttgtat ggcacgtatc atcactaaat
    20521 cgtacagtcg accaggcgtt gctacaacaa tatccattcc ttgaagtgct gcttgttgtt
    20581 gtgttgccat atttactcca ccataaacac ccaatacacg tacattcata taggcggtaa
    20641 tttgtttaat ggtatctacc acttgcacaa caagttcgcg tgtgggtacc attatcaaaa
    20701 tgcggggatt agcttgttta gagaacccta aatctcgcaa aattggcaat agataagcca
    20761 atgttttacc tgttcctgtt tgtgcaatac ctaccatatc tttgcccgag cgaattaccg
    20821 aaaaagcttc ggtttgtatc tgtgtaggtt caattaaccc catatcagca atagcgttac
    20881 gcaatggggt ggtcagttcg agttggtcaa aggtattcat aggtataaaa aatgcgttat
    20941 gcaaattgga aatcggtgca aaggtacaaa aaaaatgcta gctatgtttc ctgattttga
    21001 tatcactaat ttgctttatg attaatcagt gaagattgcc taataaaaac aataatacat
    21061 atgtatattg atatttatga tttaataatc ttaaatcggt ttcaatccta actctttcaa
    21121 atattcgttg tgtttttctg tgtttacttt tatgctcttg tttatttcag tcaatttgat
    21181 gtttacatct tccaagtcaa tttgtatttc atcttctgaa gtacttacgt atcttgaaat
    21241 gttcagatta tagtcgttct tcttgatttc gtccagtgaa actctacgag aataacgctc
    21301 tctttcattt cggaatttat aagtctcaac gatgttgtcc aggtgcttct cggaaagctc
    21361 attttggcgt tttcctttta caaattg
//
LOCUS       DAOSRP010000016        20541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2785_length_20541_cov_5.803622, whole genome shotgun sequence.
ACCESSION   DAOSRP010000016 DAOSRP010000000
VERSION     DAOSRP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20541
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2785_length_20541_cov_5.803622"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..885
                     /locus_tag="PLJ82_01975"
     CDS             400..885
                     /locus_tag="PLJ82_01975"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOR40309.1"
                     /translation="MEKIASFTIDHIRLLRGIYLSRTDLVQSETISTFDIRMKEPNRQ
                     PALSIASLHVIEHLAATFLRNHPIWAHKVIYWGPMGCCTGNYLILRGALQPIDIVELM
                     IETFQFIAHFEGDIPGMTPKDCGNCRLQDLPNAKNEAAIFVKEVLSVIQPENMIYPTE
                     K"
     gene            complement(886..2004)
                     /locus_tag="PLJ82_01980"
     CDS             complement(886..2004)
                     /locus_tag="PLJ82_01980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40310.1"
                     /translation="MKIAIVSCFYPYRGGIAQFNANLYAVLSNEHNVKAFNFTRQYPS
                     LLFPGKTQYVTDKDTALAIESERVLDSANPFSYYSTAKKVLAYKPDVIILRYWHTYFA
                     PSMGYIARYATKRGYKVITIADNIVPHETHWFDTLFTKWFLKQNTALVTLSEAVRDDL
                     VRLHPAAKHMMKRHPLYNHFGEKISAEQARKQLHIDNNKKTLLFFGLIRDYKGLDLLL
                     DAMNLLDDSYQLVIAGECYGNFDTYQRQIDTNKNKQNIHLFHRYINDSEVPMFFSAAD
                     VCVLPYKTATQSGIAAISYHFEVPLVITNTGGLKEMVETPHTGLVCERIDSDSIAETI
                     QQFFTEDKTSFIEGIQAEKKALSWENFAQSLTEFIHNI"
     gene            complement(2094..2513)
                     /locus_tag="PLJ82_01985"
     CDS             complement(2094..2513)
                     /locus_tag="PLJ82_01985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOR40311.1"
                     /translation="MKQYDYELTFKVRDYECDLQGIVNNANYQHYLEHTRHEFLLSRG
                     ISFSELHEKGIDAVIARIEIAFKYPLRSKDEFVSKLNIRKEGIKNVFYQDIYRLPDMK
                     LCIRAKAENVCLVQGELKECPELEEKLNVFYAPQLIK"
     gene            complement(2526..3155)
                     /locus_tag="PLJ82_01990"
     CDS             complement(2526..3155)
                     /locus_tag="PLJ82_01990"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOR40312.1"
                     /translation="MDNEVSNVVPSIVQGNKISYINERKLIFAAGNEFRNFDLSATRI
                     LSRRIEDISFVQTQYHALLYPDEIRKKAWYTQDYDINGRIIVNIQGTTENDTEADYFF
                     VHFSLPSTLLPEDVYLLGQFNHYHMDSSSIMKYNYEKRCYEKSLLLKQGGYDYAYVTR
                     ARESEKGTMNTIEGDYWETENEYIVYVYYRSFNDQSDRLIGKKIIRTTN"
     gene            complement(3140..3787)
                     /locus_tag="PLJ82_01995"
     CDS             complement(3140..3787)
                     /locus_tag="PLJ82_01995"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOR40313.1"
                     /translation="MKKGNYVKLIVSILLFPMCSLMANVYHTQIFDTDIHTLRVYNPN
                     QKPYYPVVDLHVNEYVELSFDDLHPSFRLFSYKIIHCNADWTVSNATEIEYAEGFSTG
                     NIEDSSPSINTYVPYTHHSIRFPNENVRFKQSGNYAIVIYTNNDEQQVALTARVYVSE
                     NSITINGTVSGITDIDYKKEHQQLSIDIIPNNFTIHNPYRDIKVIYNKTNEWTTK"
     gene            3954..5309
                     /locus_tag="PLJ82_02000"
     CDS             3954..5309
                     /locus_tag="PLJ82_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOR40314.1"
                     /translation="MAKVIHLKKGLDIKLKGAPLEIIGKATQSELIGFVPEHFAGIIP
                     KPVAKPGDKLKVGSVLFVDKNCPELRIVSPVSGELVAINRGERRKILDIVIKNDFKME
                     QEIFPTIDWKNASDKEVKQMLLSLGLFAFIKQRPYDIVANPNDQPKAIFISTFDTAPL
                     APDYTFILKDQVADFQAGITALSTLAPVSVGIKAGNGIFDGLKNATLHYFKGAHPAGN
                     VGVQINNVNPVNKGEVVWTINPQEVLFIGRYVNKGTLDFSKLVASTGSEVTTPAYYPS
                     IVGSPITPIVKANVSQGMSLRYISGNVLSGEKITENGFLNPFHSQISVIPEGDDNHEL
                     FGWIMPRWDKFSNSGTYFTKLVQFLNPKKTFEADARLLGGERHMIMSGEYDSVLPMDI
                     YPEFLIKAIIANDIDKMEALGIYEIAPEDVALCEFVCSSKVPLQKIVREGLDMLRKEM
                     N"
     gene            5338..6576
                     /locus_tag="PLJ82_02005"
     CDS             5338..6576
                     /locus_tag="PLJ82_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOR40315.1"
                     /translation="MKALRKLVDKIKPNFQEGGKLSMFHSVFDGFETFLFVPSKTSTS
                     GTHIHDAVDSKRMMSMVVIALVPALLFGMFNVGYQHFLATGQLVATYWNPQTAQFWNI
                     FLYGVALVLPKVIVSYVVGLGIEFTVAQWKKEEIHEGFLVSGILIPLIVPVDLPLWML
                     AVATAFSVIFAKEVFGGTGMNIFNVALVARAFLFFAYPTKMSGDEVWVGTNEVFGLGG
                     GQLVDSFSGATPLGQTAVATGKEFVIHNVAGATTSWWDMFVGLIPGSIGETSVIAIAL
                     GAILLLVTRVASWKTMLSVFLGGAITAFLFNTFGAESNTMAHLPWYYHLVLGGFCFGA
                     VFMATDPVTSARTEKGKWIFGFLIGAMAIVIRVLNPGYPEGMMLAILLMNIFAPLIDY
                     FFVQANISKRLKRTTKSINA"
     gene            6588..7283
                     /gene="nqrC"
                     /locus_tag="PLJ82_02010"
     CDS             6588..7283
                     /gene="nqrC"
                     /locus_tag="PLJ82_02010"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOR40316.1"
                     /translation="MNTNSNVYTIVYASVMVIIVAFVLAFFSGSLKERQDLNVKLDKK
                     KQILYSLNLDLQDKDPAALYQQYITQELIVNVNGDVVERKGGFDLDIKTELAKKDESS
                     RLLPVYECNIDGETKYIFPLYGAGLWGPIWGYIALNHDKNTVYGVYFSHAGETPGLGA
                     EITSHVFQSQFPNKKIKNTTNDLVSIAIVKPGKTAEGQDYVDGISGGTITSHGVELML
                     KNTLVNYTSFLNK"
     gene            7343..7975
                     /locus_tag="PLJ82_02015"
     CDS             7343..7975
                     /locus_tag="PLJ82_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOR40317.1"
                     /translation="MKSLFSAKNKEVIVGPLSLNNPITVQVLGICSSLAVTAKLEPAI
                     VMGMSVTIIVAISNVIISFMRNTIPVRIRIIVQLVVVATLVTVVSQFLKAFAYDVSVQ
                     LSVYVGLIITNCILMGRLEAFAMSNRPWESFLDGLGNGFGYALILVIVAFFRELLGSG
                     SLLGFHVIPASVYESGYVNNGLMVMPPMALIILAIIIWAHRTKVKQLQDN"
     gene            8001..8600
                     /gene="nqrE"
                     /locus_tag="PLJ82_02020"
     CDS             8001..8600
                     /gene="nqrE"
                     /locus_tag="PLJ82_02020"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOR40318.1"
                     /translation="MENLSSLFVKSIFVDNMIFAYFLGMCSFLAVSKNVKTAFGLGVA
                     VTFVQVMTLPVNYLLENYILKPDVLIEGVDLSFLSFIVFIAVIAGITQLVEMMVEKFT
                     PALYASLGIFLPLIAVNCAIMGGSLFMQQRNFTSISEASVFAVGSGLGFLVAIVGLAA
                     IREKMAYSHVPAPLKGLGITFIVVGLMAMAFMCFSGLKV"
     gene            8641..9903
                     /gene="nqrF"
                     /locus_tag="PLJ82_02025"
     CDS             8641..9903
                     /gene="nqrF"
                     /locus_tag="PLJ82_02025"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOR40319.1"
                     /translation="MTFLSVSPIMIGTSIVVFLAVILLLVVILLFAKKYLSPSGPVKI
                     LINGEKEIEVESGSTLLSTLANEKIFLSSACGGGGSCAQCKCQVIDGGGEILPTETVH
                     FSRKEVKDNWRLGCQVKVKNNMQIKIDEAILGVKQWECEVVSNNNVATFIKEFVVKLP
                     EGENLNFISGGYIQINIPKYAIKFSDFDIQDRFRGDWDKFNMWNLTCTNTEETVRAYS
                     MANYPAEGNIVMLNIRIATPPFDRINGGFLNVPPGIASSYIFSLKPGDKVIVSGPYGE
                     FHPLLDTGREMLYIGGGAGMAPLRSHILHLLNTLKIKDRKISYWYGARSKNEIFYEED
                     FRALEKEFPNFKFNIALSDPQPEDNWTGYVGFIHSVILNEYLSKHEAPEDIEYYMCGP
                     GPMANAVEKMLYDLGVPREMLMFDDFGA"
     gene            10068..12545
                     /gene="priA"
                     /locus_tag="PLJ82_02030"
     CDS             10068..12545
                     /gene="priA"
                     /locus_tag="PLJ82_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOR40320.1"
                     /translation="MSSNTIQMFVEVILPVNISSTFTYSVPANWQGKLTKGMRVVVPF
                     GKNKIYTGVVLEILSSHNGEFVLKEIIEILDDNQFVSNAQLAFWKWIASYYMCSLGEI
                     MVAAIPSVFRLESQSIIRLHPDFDERETYSADFSSKTQESIFQYIIQKKQLTIQQLIK
                     QTGIKKPLYAVKQLVEQNMLTITHEVTEKFLPKYVTYVRLTWIAQHKIDSIFEEIKRA
                     KKQVHLLETFIDIAQCDDSTKPIPIEKSVLLQRASVSEAVLSELVKKQVLEVYKLPVS
                     RFQETSSLTENKLVLSPDQERAYKEIVGYFETKKPVLLHGVTSSGKTEVYIQLIQDAV
                     HKDKQVLYLVPEIALTIQLLQRLEQAFGNKVGVFHSKYSDQERAEVYQNLLSPNPYKI
                     IIGVRSSIFLPFQNLGLIIVDEEHEQSYKQVDPAPRYHARNAAIMLASIIQSNVLLGT
                     ATPAIETYYNVSVKKYAYVSLASRYQNVLLPVIRVVDVADAYKKNRMKSMFSWFLIEK
                     IKEAIEQNQQVVLFQNRRGFSSHVECQQCGWVPKCMACDVSLTYHKFANYLSCHYCGY
                     TEQLPTQCPQCGNNQLKDRGFGTEKIEEELGKLFPGIRTARMDVDTTSKKNAYKKLIQ
                     AFENHQIDVLIGTQMITKGLHFDNVSLVGILNADNLFNYPDFRANERAFQMLVQVSGR
                     AGRKQKQGTVVLQTFSPHNPIVNYIVNNDYHGFFCSETEERKVFNYPPFCRLIHIFVK
                     DSDKEIVDRVVNELACHLRRSFGNRVLGPDNPPVSRIQHIYIKKIIVKIELQSSFEKA
                     KEKVQNSIASLLHQYKKTRIVIDIDPL"
     gene            12660..13127
                     /locus_tag="PLJ82_02035"
     CDS             12660..13127
                     /locus_tag="PLJ82_02035"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOR40321.1"
                     /translation="MKKILFVCLGNICRSAAAEAVMKSVVNKIGRDKDFFIDSAGIIS
                     VHKGEPADNRMRKHAARRGYAVTSISRPVNYSDFEQFDIIVGMDNTNIDDLKDKAPSV
                     EAQKKIYKMTDFCERSQYDYVPDPYYGGQDGFELVLDILEDACDGLLKKIDVL"
     gene            13162..13920
                     /locus_tag="PLJ82_02040"
     CDS             13162..13920
                     /locus_tag="PLJ82_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40322.1"
                     /translation="MKENVVTLLCFLGIMGAILMYFVFPNDITHAILFGAICLIITII
                     PALLLSRTKKKKKPIYTYTDKSFADFHPIQSDSLSMIEEVSNTTRKVSDSSLTAKRMY
                     GKVLACVADFEELNKAPLSAEGRTECVLFVSTVVLLEYKSLYQIKEYRDFEVAYLDIM
                     LSLVHQNATQKSKEEVYEFVKRRLRFYTAEYQTLIDNKFPLEFYSYFFYTPLRTRTQV
                     CLDQNELIRFHGNLLSLVEKIKLSTKNDASFLGV"
     gene            complement(13909..14583)
                     /locus_tag="PLJ82_02045"
     CDS             complement(13909..14583)
                     /locus_tag="PLJ82_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR40323.1"
                     /translation="MNLIETQGVCKSFGTLQVLKNIDLTIRKGEFASIIGASGAGKTT
                     LLQIIGTLDKPTAGTITMNKVKLESLHSDELSELRNKEIGFVFQFHQLLPEFTALENV
                     MIPALIAKVQKKEAEKKAQELLHYLGLTNRLLHKPNELSGGEKQRVAVARALINNPSI
                     ILADEPSGSLDSRNKEELHQLLVTLNRDLGITLLVVTHDTNLAYLSNRIIEMQDGIII
                     GEKLNA"
     gene            complement(14580..15293)
                     /locus_tag="PLJ82_02050"
     CDS             complement(14580..15293)
                     /locus_tag="PLJ82_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOR40324.1"
                     /translation="MWTSRTQLLLGEKNSSILEKKHVMIVGVGGVGAYAAEMICRAGI
                     GNITIIDADTIKESNINRQLPALHSTIGKKKVTVLTERLLDINPNLVLKQHDVFIEKE
                     LIPSLFENLHVDFVIDAIDTLSPKIDLIRYCIQHKIKIVSSMGAGGRTDPSKIHYADI
                     SGTYHCTLAKHVRKRLKLVNISKGLPVVFSSEQVNQNSVIQIDDEKNKCSTVGTISYL
                     PAIFGCYLAAYVIKKLIQK"
     gene            complement(15301..16626)
                     /locus_tag="PLJ82_02055"
     CDS             complement(15301..16626)
                     /locus_tag="PLJ82_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOR40325.1"
                     /translation="MQWETLLSTKRFGMEAYHKKDTEDRSEFQRDYDRLIFSSPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVACVGRSLGTNIAHQLSILHQKSSNFLPEIGSIV
                     SAACLAHDLGNPPFGHSGEKAIASFFSEGNGSFIKERLENETEWNDLIFFEGNANSLR
                     LLTHQFKGRRYGGFAMTYSTLASIVKYPYSSVECGKKGKFGFFESEKATYEQIAMELG
                     IIKSPVSDKKYARHPLVYIVEAADDICYQIMDIEDAHKLKILSTEQTKELLLQFFDAE
                     RKKRIYETWKLVDDSNEQISYLRSSVIGKLVSECARVFVENEQQILSGQFSGSLIDHI
                     EPVCKNAYENCSTIAVKKIYKSQEVLDIEVAGYKIIQTLLDEYIKASFQPNKSYSKLL
                     LDRVPSQYDMYAPSTYEKIQAIVDFISGMTDIYALDLYRKITGMNLPSL"
     gene            complement(16654..17376)
                     /locus_tag="PLJ82_02060"
     CDS             complement(16654..17376)
                     /locus_tag="PLJ82_02060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOR40326.1"
                     /translation="MTQTYIHKDLGKILIYYSPKARSIRVQVGDNGIKLIIPLGVNID
                     KGLHFVNAKQDWIEKALKRTEQKKRHVSIHITELNSQTFSILTKACERTNILFRLHEK
                     ILTIEHPISLDITQAKYQQILLDGMEKFVVSEAKKHFSQRLAYLANKGGFTYNEFRIQ
                     RSKTRWGSCSSKKNINLCAYLLFVPAYLSDYVIIHELCHTKEMNHGNTFWNLVASQEP
                     NYITYRKEIKQYAFLMQLFNRK"
     gene            17524..17994
                     /gene="rimP"
                     /locus_tag="PLJ82_02065"
     CDS             17524..17994
                     /gene="rimP"
                     /locus_tag="PLJ82_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOR40327.1"
                     /translation="MISKDVVVQTVNQFLKDKEDYFLVDVTISIHNTICVEIDAFQGV
                     DVETCCELSRFIETKIDREIEDFELEVGSAGITAPFKILQQYIKNINNEVEVLLKNGK
                     KIIGFLRSANEHTFVVEVSQKIKKDKNAKKEEVTELLTYAYSDVKHTKYNLRFK"
     gene            18000..19253
                     /gene="nusA"
                     /locus_tag="PLJ82_02070"
     CDS             18000..19253
                     /gene="nusA"
                     /locus_tag="PLJ82_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOR40328.1"
                     /translation="MSNKPVQSLNLIETFSEFKELKNIDKATMISVLEDSFRSVIAKM
                     FGTDESFTVVINPDKGDCEIWRDYEVVADGEVEDEVLQMGLSKARAIDDECEIGDDVT
                     EAVNFLSFGRRAVLTLRQTLASKILELQKESVYDQYKEMVGQVVYGEVYQIWKKEILL
                     LDDQGNELLLPKTEQIPTDFYKKGDTVKSVVVRVDNKNNQLKIIVSRTAPIFLQRLFE
                     QEIPEVQEGTIMIRRIARIPGERAKVAVESYDDRIDPVGSCVGVKGSRIHSIVRELRN
                     ENIDVINYTSNLSLFIARSLSPAKVLSIRIDEERKRAEVSIKPEDVSMAIGKGGSNIK
                     LATMLTEYTIEVFRHVDEEDEDDVFLDAFSDEIEPWVIDVFKSIGCDTAKSVLEIPRQ
                     ELIDRTDLEEETIDEALAILRQEFE"
     gene            19338..>20541
                     /locus_tag="PLJ82_02075"
     CDS             19338..>20541
                     /locus_tag="PLJ82_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR40329.1"
                     /translation="MSTKLNKVVKELNVGLATAVEFLHKKGFADVESNPNARITEEQY
                     DLLVKEFSKDKSQKIESGILAQRSKERPKGEVVALEGFDKENKPVEEIKVEIQEASRP
                     QFKQVGKIDLDALVPKKKDTNEVKEEMPNIDVPLQENVEKIVTVQQVEQAPIQEVEEE
                     ITEPSVVVEAEIEDKSETENVVEDIDESVDDTISAELSDDVYTIEKNTIHSTINVVGK
                     IDLDALNQQTRPKKKTKEEKKKERLEREIQKPTPTVATAPKDAVQKEATVAADKKKRS
                     RIQRERVNIYDPNAQAKKPTKPQPQANNKEVKKFVPKEQISDEDIQKQIKETLARLTN
                     KGKSKGAKYRKDKREAVSQRLQEEADLERLESRVIKITEFVTANELATMMDMPVTQVI
                     GTCMSIGIM"
BASE COUNT         6627 a         3176 c         3898 g         6840 t
ORIGIN      
        1 gtcggtagtg tattacatat tgctattgct gggcggagtt acccctagtt cgtcgtaggc
       61 tgtgcctacg aaaagcaaag ctctcaaacg aatgtactgt ggagagttga aagttgacaa
      121 ttgatagttg ttaattctgc acgaactacg aacaacacta aacgctaaac actaaacact
      181 aaacactaaa cacctctgtt acccttagct cgccattgcc tcttcagggc gttttaggta
      241 ggctaaaaat caacctaatg gcggaagtcg atttgtaatc ggtggataca gttctaaagt
      301 gaaaaaactc tatccttttt tttttaatta tattttagtc gctaaaacaa ataatacttc
      361 tatctttgta tcttaaaaca atatttatca tatatatata tggaaaaaat agccagtttt
      421 actattgatc atattcgtct gttgagaggt atttaccttt cgcgtactga tttagtacaa
      481 agcgaaacca tcagtacctt tgatatacgc atgaaagaac ctaatcgtca acctgctttg
      541 tctattgcat cgctacatgt aatagagcat ttggcagcta cgtttttacg caatcatcct
      601 atatgggcac acaaggtaat ttattgggga ccgatgggtt gttgcacagg caattatctt
      661 atacttcgtg gagcattgca acccattgat attgttgaat taatgataga aacttttcaa
      721 tttattgctc attttgaggg ggatattcca ggtatgactc ccaaagattg tggtaattgc
      781 aggttgcaag atttacccaa tgcaaaaaat gaagccgcaa tctttgtaaa agaagtatta
      841 tcagttatcc aaccagaaaa tatgatttat cccactgaaa agtaactaga tattgtgtat
      901 aaattcggta agcgattgtg caaaattttc ccacgaaaga gcctttttct ctgcttgtat
      961 accttctata aaggacgttt tatcttcggt gaaaaattgt tggattgttt ctgcaatact
     1021 atcactatca attcgttcgc acactagtcc agtatgggga gtttccacca tttcttttag
     1081 tcctcctgta ttggtaatta ctaggggtac ctcaaaatga tacgaaatgg cagcaatgcc
     1141 actttgtgta gctgttttgt agggcaatac gcatacatca gcagccgaaa aaaacatagg
     1201 cacttcgctg tcgttgatat aacgatgaaa aaggtgtatg ttttgtttgt ttttgttcgt
     1261 gtctatttgt cgttggtatg tgtcaaaatt tccataacac tcgcctgcaa tcactaactg
     1321 ataactgtca tcaagtaggt tcatggcgtc gagcaataag tctaagcctt tgtagtcgcg
     1381 tattaatcca aaaaatagca aggttttttt gttgttatca atgtgtagct gttttcgagc
     1441 ttgttctgcc gatatttttt caccaaaatg attgtatagt gggtgccgtt tcatcatgtg
     1501 tttagcagca ggatgcaaac gaactaaatc gtctcgtacg gcttcgctaa gtgttaccaa
     1561 tgctgtgttt tgtttcaaaa accatttggt aaaaagtgta tcaaaccaat gtgtttcgtg
     1621 aggcacaata ttgtcggcaa tagtgattac tttgtatcca cgtttggtag catagcgagc
     1681 aatatacccc atcgaagggg caaaataggt gtgccaataa cgtaatatga ttacatcagg
     1741 cttataagca agcacttttt ttgccgtaga ataataggaa aatgggtttg ccgaatcgag
     1801 tacacgttcg ctttctattg ccaaagcagt atccttgtcg gttacatatt gtgtttttcc
     1861 aggaaacaac aatgatgggt attgtcttgt aaaattaaat gcctttacgt tatgttcgtt
     1921 acttaaaaca gcgtataaat ttgcattgaa ttgtgcaata cctcccctat acgggtaaaa
     1981 acaagatacg atggcaatct tcattttcgt agaatacaat tagaatacaa aagtagtaaa
     2041 aaaatatcag attaaagatg aagtgttaag gtctttttgt tcaaacagtc tgtttatttg
     2101 ataagttgtg gagcgtaaaa tacgtttaat ttttcttcta attcggggca ttcttttagc
     2161 tctccttgta ccaagcatac attttctgct ttggctctaa tgcacagctt catatcaggc
     2221 aagcgataaa tatcttggta aaatacattt ttaattcctt cttttcgtat gttaagtttg
     2281 cttacaaatt catctttgct tctgagtggg tacttaaagg caatttcaat gcgggcaata
     2341 accgcatcaa ttcctttttc gtgcaattct gagaaactta ttcctctaga tagcaaaaat
     2401 tcatgtcgtg tatgttctaa ataatgttgg tagttagcat tatttactat cccttgcaaa
     2461 tcacattcat agtctcgcac tttgaaagtt agttcataat catattgttt catatcaatg
     2521 taatgttaat tggttgtccg aattattttt tttccaatta ggcggtcgct ttggtcgtta
     2581 aaacttctgt aatatacata cacaatatat tcattttctg tttcccaata gtctccttct
     2641 atggtgttca ttgtcccttt ttcgctttcg cgagcacgtg ttacataggc ataatcatat
     2701 cctccttgtt ttaaaagtaa tgatttttca tagcatcttt tttcgtaatt atacttcatt
     2761 atagaagagc tgtccatgtg ataatgattg aactgtccca gtaggtatac gtcttcggga
     2821 agtagtgtag agggcaacga aaaatgaaca aaaaaataat ctgcttcggt gtcattttca
     2881 gttgtacctt gtatattcac aattattctg ccgtttatgt cgtaatcttg tgtgtaccac
     2941 gcttttttgc gtatttcgtc ggggtagagt aatgcatgat actgcgtttg tacaaaagaa
     3001 atatcctcta ttcgacgact taaaatccgt gtggcagaca aatcaaaatt acgaaattcg
     3061 ttgcctgctg caaaaatgag tttacgttcg ttgatatagc taattttgtt gccttgaacg
     3121 atactgggta ctacattact tacttcgttg tccattcgtt ggttttgttg taaattacct
     3181 ttatatcgcg gtaaggatta tggatagtaa aattgttcgg tataatatct atactaagtt
     3241 gttgatgctc ttttttataa tcaatatcag tgattcctga cactgttcca ttgattgtaa
     3301 tactattttc cgatacatac acacgtgctg ttagtgctac ttgttgctca tcgttgttag
     3361 tataaattac gatagcataa tttcccgatt gtttaaatcg tacattctcg tttgggaaac
     3421 gtattgagtg gtgtgtataa ggcacataag tgttgattga tggactcgaa tcttctatgt
     3481 ttccagtaga aaaaccttcg gcgtattcta tttctgtggc attcgatact gtccaatcag
     3541 cattgcaatg tataatttta tacgaaaaaa gacgaaacga tgggtgcaaa tcgtcaaacg
     3601 aaagctcaac gtattcattt acatgtaaat caacaaccgg gtagtaaggt ttttggttag
     3661 gattgtatac gcgtaatgtg tgaatatcag tatcaaaaat ctgggtgtga taaacattcg
     3721 ccataagaga gcacatcgga aaaagcaata tgctcactat caattttaca tagtttccct
     3781 ttttcattgt tggcaaagat ataattaaaa aatgaaactg caaactgtct ttttgatact
     3841 atttattatc tgtaaaaaat aaagtgttaa aaagggaact tttatcttgt acatatcgtt
     3901 attttttcta attttgcgaa gtttttacaa aaacagtatt caacctcatt tttatggcta
     3961 aagtaataca tctaaaaaag ggcttagata ttaaactcaa aggtgctcct cttgagataa
     4021 taggaaaagc gacacaatcg gagttgattg gttttgttcc tgaacatttt gcaggaatta
     4081 ttcccaaacc cgtagctaaa cctggtgaca aactgaaagt aggttcagta ttatttgtgg
     4141 ataaaaattg tccagagtta cgaatagttt ctccagtaag tggcgaatta gtagctataa
     4201 acagaggaga acgtcgtaaa attttggata ttgttatcaa aaacgatttc aagatggagc
     4261 aagaaatttt tcctactatt gattggaaaa atgcttcaga caaagaagta aagcaaatgc
     4321 ttttatcact cggcttattt gcttttatca agcaacgtcc atatgatatc gtggctaatc
     4381 caaatgatca acccaaagcc atttttatct ctactttcga taccgctcca ttggctcccg
     4441 attacacttt tattttaaaa gatcaagtag ccgattttca agcaggtatt acagccttgt
     4501 ctaccttagc acctgtctct gtagggatta aagcaggcaa tgggatattt gatggtttga
     4561 aaaatgcaac cttacattat tttaagggag ctcatcctgc tggtaatgta ggtgttcaaa
     4621 tcaataatgt gaatccagta aacaaaggag aggttgtttg gactatcaat cctcaagagg
     4681 tattgtttat tggtcgatat gtaaataaag gtaccttaga tttctcaaaa ttggtagcat
     4741 ctactggttc tgaagttaca acgccagcgt attacccatc aattgttggt tctccaataa
     4801 caccgattgt aaaagcaaac gtaagccaag gtatgtcttt acgctatatt agtggtaatg
     4861 tgctttctgg tgaaaaaatt actgaaaatg gctttttaaa tccttttcat tctcaaatct
     4921 cagttatacc tgaaggagat gataatcacg aattgttcgg ttggataatg ccacgttggg
     4981 ataaatttag caatagtggt acatatttta ctaaactagt gcaattttta aacccgaaaa
     5041 aaacattcga agcagatgca cgattgttag gaggcgaacg acatatgata atgagtggag
     5101 agtatgatag tgtattgcca atggatatat atcctgaatt cttgattaaa gccatcattg
     5161 caaatgatat agataagatg gaagccttgg gaatttacga aatagcacca gaagatgttg
     5221 cattatgcga atttgtttgc tcgtctaaag ttcccttaca aaaaattgtg agagaaggat
     5281 tggatatgtt gcgaaaagaa atgaattgat tattaatata tttaaatacc taatacattg
     5341 aaagcactta gaaaattagt cgataaaatt aaacccaact tccaagaagg tggtaaatta
     5401 tcgatgttcc attccgtttt tgatgggttt gaaacctttt tgtttgtgcc aagcaaaaca
     5461 tcaacatctg gcacgcatat tcacgatgct gttgactcta aacgtatgat gtcgatggtt
     5521 gttatagcat tagttcctgc tttactgttt gggatgttta atgttggtta ccaacatttt
     5581 ttagccacgg ggcagctcgt tgctacatat tggaatccac aaacagctca attttggaac
     5641 atctttttgt atggagtagc attggtatta cccaaagtta tagtttcgta tgttgttggt
     5701 ttgggtattg aatttactgt agctcaatgg aaaaaagaag agatacatga aggattttta
     5761 gtgtccggaa tacttattcc tcttatcgtt cctgttgatt tacctctttg gatgttagcc
     5821 gttgctactg cattctccgt aatttttgca aaagaggtgt ttggaggaac aggaatgaat
     5881 attttcaacg tagcattggt ggcacgtgca tttttgttct ttgcgtatcc taccaaaatg
     5941 tcaggagatg aggtatgggt tggaacaaat gaagtgtttg gtcttggcgg tggacaatta
     6001 gtggattctt tttcaggagc cactcctttg gggcaaacag ccgttgcaac aggcaaggaa
     6061 tttgtgatac ataatgtggc aggtgctact acttcgtggt gggatatgtt tgttggtcta
     6121 attcctggat cgattggtga aacaagtgtt attgctatag cattgggagc tatccttttg
     6181 ctcgttaccc gtgtagctag ctggaaaaca atgttatccg tctttttagg cggagctata
     6241 acagcttttt tatttaatac attcggagca gagtcaaata ctatggcaca cttaccttgg
     6301 tattatcatt tagtattagg aggattttgt tttggagctg tatttatggc taccgaccca
     6361 gttacatcag cacgtactga aaagggaaaa tggatatttg gctttttaat cggtgccatg
     6421 gctattgtta tccgtgtgct caatccagga tatcccgaag gaatgatgtt ggctatttta
     6481 ttgatgaata tttttgctcc tctcattgac tatttctttg tgcaagcaaa tattagtaaa
     6541 cgccttaaac gtacaacaaa atctattaac gcataaaaac tagaacaatg aatacaaata
     6601 gcaatgttta tacaatcgtt tatgcctcgg taatggttat tattgtagca tttgttcttg
     6661 cttttttctc tggttcgctc aaagaaagac aagatttaaa tgtaaaatta gataaaaaga
     6721 aacaaatttt atattcttta aatttagatt tgcaagataa agatcccgca gccctctacc
     6781 agcaatacat tacacaagag cttattgtta atgttaatgg cgatgtggta gaaagaaaag
     6841 gaggttttga tcttgatatt aaaacagagt tagcaaaaaa agatgaatcg tctcgtttat
     6901 tgcctgtata tgaatgcaat atagatggag aaactaaata tattttccct ctttatggtg
     6961 ctggtttatg gggaccaatt tggggatata ttgccttaaa tcatgataaa aataccgtgt
     7021 atggagtgta tttctctcat gcaggagaaa ctccaggctt aggagctgag ataacatctc
     7081 atgtgttcca atcacaattt ccaaacaaaa agataaaaaa taccacaaac gatttggtgt
     7141 caatagccat tgtaaaacca gggaaaacgg cagaaggtca agattatgtg gatggtattt
     7201 ctggtggaac aatcacatct catggggtag agcttatgtt aaagaataca ttagtaaact
     7261 atacaagttt tttaaataaa taattagcac atagcatctt ttctttttag agagtagatg
     7321 tgaaaattaa aataataagc gtatgaaaag tctgttttct gcaaaaaata aagaggttat
     7381 tgtaggccca ttgagtttga ataatccaat tactgtacaa gtattaggta tatgttcttc
     7441 cttagctgta acagcaaaac tcgagcctgc tattgtaatg ggaatgtctg taactattat
     7501 tgttgccata tcaaatgtta ttatttcttt catgcgaaat accattcctg tgcgtattcg
     7561 cattatagta caacttgttg ttgtagctac cttagtaact gtcgtaagtc aatttctaaa
     7621 ggcttttgcc tatgatgtaa gtgtacagtt gtccgtatat gtaggattaa ttattactaa
     7681 ctgtatattg atgggacgtt tagaagcatt tgctatgtct aatagaccct gggaatcttt
     7741 tttagatgga ttgggtaatg ggtttggtta tgcattaatt ttggttattg tagccttctt
     7801 tcgcgaattg cttggttctg gttcattact tggattccat gttatccctg caagtgttta
     7861 cgaatcgggt tatgtcaata atggtttaat ggtaatgcct ccaatggcac tgattatttt
     7921 agctattatt atttgggctc atcgtacaaa agtaaaacaa ttacaagata attaattaca
     7981 acctagtaaa ttcagaacga atggaaaatt tatcaagctt atttgtcaag tcgatttttg
     8041 tcgataatat gatttttgcc tatttcttgg gaatgtgttc ttttttagcc gtttctaaga
     8101 atgttaaaac tgcttttggt ctaggtgtag ctgtaacgtt tgtgcaggtt atgaccttac
     8161 ctgttaacta tttattggag aactatatac ttaaacccga tgttttaatc gaaggggtag
     8221 atttgagttt tttaagtttt atcgtcttta ttgctgttat cgcgggtatt acacaattgg
     8281 tagaaatgat ggtggagaaa tttactcctg ctctatatgc ttctttagga attttcttac
     8341 ctttaattgc tgtaaactgt gctatcatgg gtggctcttt gtttatgcaa caacgtaatt
     8401 ttacttccat tagcgaagca tctgtttttg cagtaggttc ggggttaggt ttccttgttg
     8461 caattgttgg attagctgca attagagaaa aaatggcata ttcacatgta ccagcacctc
     8521 taaaaggctt gggaattaca tttatcgtag tagggttgat ggcgatggca ttcatgtgtt
     8581 tttctggctt aaaagtctaa taaatttaag atacaaaaca gcatttaaaa aaataactat
     8641 atgacatttt tatctgtaag tcctattatg ataggcacta gtatagtggt atttttagcg
     8701 gtaattcttt tactcgtagt aatcttatta tttgctaaaa aatatttatc tccatcaggt
     8761 cctgttaaaa ttttaattaa cggagaaaaa gagatagaag ttgaatctgg atctacatta
     8821 ttgtctacat tggcaaatga aaagatattt ttatcatctg cgtgtggagg aggtggtagc
     8881 tgtgctcagt gtaaatgtca ggttattgat ggtggtggtg aaattttacc aacagaaaca
     8941 gttcactttt ctcgtaaaga agtaaaagat aattggcgtt taggttgtca agtaaaggta
     9001 aaaaacaaca tgcaaatcaa aattgatgaa gctatcttag gagtgaagca gtgggaatgc
     9061 gaggttgttt ctaacaataa cgtggctact tttatcaaag aatttgttgt taaattgccc
     9121 gaaggagaaa atttaaactt tatttctgga ggttatattc aaattaatat acccaaatac
     9181 gcgattaagt tttctgattt tgatattcaa gatagatttc gcggtgattg ggataaattt
     9241 aatatgtgga atttaacatg taccaatact gaggaaacag tacgtgcgta ttctatggca
     9301 aattatcctg ctgaaggaaa tattgtgatg ttaaatattc gtattgctac accacctttt
     9361 gatagaatta atggaggttt tttgaatgtg cctcctggta ttgcgtcatc gtatatcttt
     9421 tcactaaaac ctggcgataa agtaattgtt tcaggtcctt atggagagtt tcatcctcta
     9481 cttgacactg gtagggaaat gttatatatc ggtggaggtg ctggtatggc tcctttacgt
     9541 tctcacattt tgcatcttct taatacgttg aaaattaagg atagaaagat ttcttattgg
     9601 tatggtgctc gttctaaaaa cgaaatattc tacgaagaag attttcgagc attagagaaa
     9661 gaattcccta atttcaaatt taatattgca ttaagtgatc ctcaacctga agacaattgg
     9721 acaggttatg tcggatttat tcatagtgtg atattgaatg agtacctaag taaacatgag
     9781 gctccagaag atattgagta ttacatgtgt ggacctggac caatggcaaa tgctgttgaa
     9841 aaaatgttat acgacttagg tgttcctcgc gaaatgctaa tgttcgatga ttttggtgct
     9901 taaataattg aacgcatatt ttttactaag aggctatcgt aaagatagtc tcttttgttt
     9961 ttaactcatt ttattatttc atatctgtta tgttgaaggt atatagcaaa caaaaatctt
    10021 ttttccacgt agtttctttt cttatctttg taataaattc gtaaactatg tcttcgaata
    10081 ctatccagat gtttgtggaa gtaattcttc ctgtcaatat ctcttcgact tttacctatt
    10141 cggttccagc taactggcaa ggtaaactta cgaaagggat gagggttgtc gtgccatttg
    10201 gaaaaaataa aatttataca ggcgttgttc tagagatact ctcttcgcat aatggtgaat
    10261 ttgtactaaa agaaataatt gagattctag acgataatca atttgttagt aatgctcaac
    10321 tggcattttg gaaatggatt gcgtcgtatt atatgtgctc gttgggcgaa atcatggtgg
    10381 ctgctattcc ttcggttttt cgattagaaa gccaatcaat catacgattg catcctgatt
    10441 ttgatgaaag agaaacgtac agtgctgatt tttcatctaa aacgcaagaa tctattttcc
    10501 agtatattat acaaaaaaag cagcttacga tacagcaact tattaaacaa acaggtataa
    10561 aaaaaccatt gtatgcagtt aagcaattgg tagaacaaaa tatgttgacg attacccatg
    10621 aagtaaccga aaaatttcta ccaaaatatg ttacgtatgt gcgattaaca tggatagctc
    10681 aacataagat agactcaatt tttgaagaaa taaaacgggc aaaaaaacaa gtgcacttgc
    10741 tcgaaacctt tatagatatc gctcaatgtg atgatagcac aaagcccatc cctatagaaa
    10801 agtctgtttt gcttcaacga gcttctgttt cagaagctgt gctatccgaa ttggtaaaaa
    10861 aacaggtatt agaagtatat aaattacctg tttcacgttt tcaagagact tcatcattaa
    10921 ccgaaaataa gcttgtatta tcgcccgatc aagaaagagc atacaaagag attgttgggt
    10981 attttgaaac gaaaaaacca gtattattac atggagttac ctcaagtgga aaaacagagg
    11041 tatatattca attaatacaa gatgctgtac ataaagataa acaagtgttg tatttagtgc
    11101 cagaaatagc gttgactata caattattgc aacgtttgga gcaagctttt ggtaacaagg
    11161 taggggtgtt tcattcaaaa tattctgatc aagaacgtgc ggaggtatat caaaatttac
    11221 tgtcgccaaa cccctataaa attattattg gagttcgttc atctattttt ttgccttttc
    11281 aaaatcttgg tcttattatt gtagatgaag aacacgaaca atcttataaa caagtagatc
    11341 cagctccacg ataccatgct cggaatgctg caattatgtt ggcttctatt atccagagta
    11401 atgtattact gggtactgcc actccagcta ttgaaacata ttacaatgta agtgtaaaaa
    11461 aatatgccta tgtgtctctt gcttcgaggt atcaaaatgt attgttgcca gtaattcgtg
    11521 ttgttgatgt agcagatgcg tataaaaaga atagaatgaa atcgatgttt tcttggttct
    11581 taattgaaaa aattaaagag gctattgaac aaaatcagca ggtggtactg tttcagaata
    11641 ggagaggatt ttcatcccat gttgaatgtc aacaatgtgg ttgggtgcca aagtgtatgg
    11701 catgtgatgt aagtcttaca tatcacaaat ttgctaatta tttatcgtgt cattattgtg
    11761 gttacacgga acagttgcca actcaatgtc cccagtgtgg aaataatcag ttgaaagata
    11821 gaggttttgg tacagaaaaa attgaagagg aattaggtaa attatttcca gggattcgaa
    11881 ctgctcggat ggatgtagat actacttcga aaaaaaatgc ttacaagaaa cttattcagg
    11941 cgtttgaaaa tcatcaaata gatgtgctta tcgggacaca aatgataacg aagggtttgc
    12001 attttgataa tgttagttta gtaggaattt taaatgcaga taatttgttt aattatcctg
    12061 attttagagc caacgaaagg gcttttcaga tgcttgttca agtaagcggt agagctggga
    12121 ggaaacaaaa acagggaact gtagtacttc aaacattttc tccacacaat ccgattgtaa
    12181 attatattgt aaataatgat tatcatggtt ttttttgttc cgaaaccgaa gagcggaaag
    12241 tatttaatta tccaccattt tgcagattaa tacacatctt tgttaaagat agtgataagg
    12301 agatagtaga tagagtggta aacgaactag cttgtcattt acgccgtagt tttggcaata
    12361 gggttttagg tccagataat ccgcctgtat caagaattca acacatatac attaaaaaaa
    12421 ttattgtaaa gatagagttg caaagttcgt ttgaaaaagc aaaggaaaag gtgcaaaata
    12481 gtatagcgtc acttttacat caatataaaa aaactagaat tgttattgat atagatcctt
    12541 tatagtgata taacgaatta agcgaatgat aaagattttg tttaaaattg ttataattca
    12601 gtattatttc ttatttttgc agtagcaatc tctatctctg taaaaagtta gtatacaaaa
    12661 tgaaaaaaat tttatttgta tgtttgggta atatctgtcg ttctgctgcc gctgaagctg
    12721 taatgaagtc tgttgtaaac aaaattggaa gagataaaga tttttttatt gattcggcag
    12781 gtataatatc agtgcataaa ggagagcccg cagataatcg aatgcgtaaa cacgcagccc
    12841 gccgagggta tgctgtaaca agtatttcta gaccagttaa ttatagcgat tttgaacaat
    12901 ttgatataat tgttggtatg gataatacca atatagatga tttgaaagat aaagctccat
    12961 cagtggaggc acaaaagaag atttataaaa tgactgattt ttgtgaacgt tctcaatatg
    13021 attatgtgcc agatccatat tatggaggac aagatggatt tgaattagta ttagatattc
    13081 tggaagatgc ttgtgatgga ttattgaaaa aaatagatgt gttatagggt gcagagtata
    13141 taactttata tagggaaata aatgaaagaa aatgttgtta ctttgttgtg ttttttaggc
    13201 ataatgggag cgattttaat gtattttgtt tttcctaatg atattacgca tgctatttta
    13261 ttcggagcta tatgtttaat tattaccatt ataccagcat tattgcttag tagaactaag
    13321 aaaaagaaga agcctatata cacctataca gataaatctt ttgctgattt tcaccctata
    13381 cagtccgatt cattaagtat gattgaagag gtttctaata caactcgcaa agtctctgat
    13441 agttctctta cagcaaaaag aatgtatggg aaagtgcttg cttgtgtagc cgattttgaa
    13501 gagttaaata aagctccact gagtgcagaa ggtcgtacgg aatgtgtgct ttttgtgtca
    13561 acggtagtgt tgctcgaata taaatccttg tatcaaataa aggaataccg tgattttgaa
    13621 gtggcatatt tggatattat gctttctctt gtacaccaaa atgcaacaca aaaatcgaaa
    13681 gaagaagtgt atgaatttgt aaaacgtaga ttgcgttttt atacagccga atatcaaact
    13741 cttatagata ataaattccc tctagagttt tattcgtact ttttttatac ccctttacgt
    13801 acacgtacac aagtttgcct tgatcagaat gaattaattc gttttcatgg aaatttgttg
    13861 tctttggtcg aaaaaattaa gctgtcaaca aaaaatgatg caagcttttt aggcgtttag
    13921 cttttctcca ataataatgc catcttgcat ctcaattatt ctgtttgaca gataagctaa
    13981 gtttgtgtca tgtgttacta ctaatagggt gattcctaaa tctctgttta atgttactaa
    14041 aagttgatgt aactcttctt tatttcttga atctaaactg cccgaaggtt catccgcaag
    14101 aataatggag ggattgttaa ttaaagctct tgcaacagca acacgttgtt tctctcctcc
    14161 tgatagttcg ttgggtttgt gtaacaatcg attagtaaga cctagataat gtaataattc
    14221 ttgggctttt ttttcagcct cttttttttg tacttttgca attaatgcag gtatcattac
    14281 attttccaaa gccgtaaatt ctggaagtaa ttgatgaaat tgaaacacaa aaccaatttc
    14341 tttatttcgt aattcagaaa gttcatcact atgtaggctt tccagtttca ctttattcat
    14401 tgtaattgtt ccggcggttg gtttatcaag tgttccaata atttgcagaa gtgtcgtttt
    14461 tccagctcca ctggctccaa taatgctagc aaattctcct tttctaattg ttaaatcaat
    14521 attttttagt acttgcaaag tgccaaaact tttacaaaca ccctgtgttt caattaaatt
    14581 catttttgta tcaatttttt tattacataa gcagctaaat agcacccaaa gatagcaggt
    14641 aaatatgaaa ttgtgcctac ggttgaacat ttattttttt catcatcaat ttgtatcaca
    14701 gaattttggt taacttgttc tgacgaaaat accacaggca atccttttga gatatttacc
    14761 aactttaatc tttttcttac atgttttgca agagtacaat ggtatgtgcc tgaaatgtca
    14821 gcataatgta ttttgcttgg atctgttctg cctccagcac ccattgagga aacaatcttt
    14881 attttatgtt gaatacaata acgaatgagg tcaattttag gggatagtgt atcaatagca
    14941 tcaatcacaa aatctacatg aagattttca aacagagagg gtataagttc tttctcaata
    15001 aatacatcat gttgtttgag tactaaatta ggattgatgt caaggaggcg ttccgttaat
    15061 acggttactt tttttttgcc tattgtgcta tgtaaagcag gaagttgcct gttaatattg
    15121 ctttctttta tcgtgtctgc atcaataata gtgatgttgc ctatgcctgc acgacaaatc
    15181 atttcggctg catatgcacc tacacctcca acacctacta tcataacgtg ttttttctca
    15241 aggatactac tatttttctc ccccagtaat aattgagttc tactagtcca catactatta
    15301 ttataatgaa ggtaaattca tgcctgtaat tttacggtac aagtctaatg cgtaaatatc
    15361 tgtcataccc gatataaaat ctacaatagc ttgaattttt tcgtaagtac ttggagcgta
    15421 catgtcgtat tggcttggaa ccctatccaa taataatttt gaataggact tatttggttg
    15481 aaaacttgct tttatgtatt catcaagtaa agtttgaata attttatagc cagctacttc
    15541 tatatctaat acttcttgtg atttgtatat ttttttgaca gctattgtag agcagttttc
    15601 ataagcattt ttacagactg gttcaatgtg atctattaat gatccagaaa attgaccaga
    15661 aagtatttgt tgctcatttt ccacaaatac acgtgcacac tcagatacta attttccaat
    15721 cacagaagag cgaagataag aaatttgttc gtttgagtcg tcaactaatt tccatgtttc
    15781 atagatgcgt ttttttcgtt ctgcatcgaa aaattgcaat aataattctt ttgtttgctc
    15841 tgttgataat atctttagct tatgtgcatc ttctatatcc attatctgat aacagatatc
    15901 gtcagcagct tctactatat aaactaaagg atgtcgtgca tattttttat cagaaactgg
    15961 gcttttgatt atacctagtt ccatagcaat ttgctcatag gttgctttct ctgattcaaa
    16021 aaaaccaaat ttcccttttt tgccacattc gacagatgaa taggggtatt ttacgatcga
    16081 agctaatgtg gaataagtca ttgcaaaacc accatatcgc ctacctttaa attgatgcgt
    16141 taacaatcga agagaatttg catttccttc aaaaaaaatt aaatcattcc attcagtttc
    16201 attttcaagc ctttctttta taaaagaacc atttccttcg gaaaaaaaac tagcaatagc
    16261 tttttcgcca gagtgtccaa aaggaggatt gcccaaatca tgtgctaaac aagctgctga
    16321 tactatcgat cctatctcgg gtaaaaaatt ggagcttttc tgatgtaaaa tgcttaattg
    16381 gtgagcaata ttagtgccta atgaccgacc aacacaggca acttccaagc tatgtgttaa
    16441 cctattatga acaaatacgc taccaggaag agggaaaact tgtgttttat tttgtaaacg
    16501 cctaaaagga gatgaaaaga tgagtctatc ataatccctc tgaaattcag accgatcttc
    16561 tgtgtctttt ttatgatacg cttccatccc aaaacgtttg gtagataaaa gagtttccca
    16621 ttgcattgtt gattgtttca tatgtataat gttctatttt ctgttgaata attgcattaa
    16681 aaacgcatat tgttttatct cttttcgata agtaatataa ttaggttctt ggcttgccac
    16741 taaattccaa aatgtatttc cgtgattcat ttcttttgta tgacaaagtt catgaatgat
    16801 aacatagtcg ctaagatatg ctggtacaaa aagtaaatac gcacataaat tgatattttt
    16861 tttggaggaa caactacccc aacgtgtttt tgaacgttga atgcgaaatt cgttatacgt
    16921 aaatcctccc ttatttgcta aataagctaa tcgttgcgaa aaatgtttct ttgcttctga
    16981 aactacaaat ttttccattc catcaagtaa tatctgttgg tatttagctt gtgtaatatc
    17041 caaagaaatt ggatgttcaa tcgttaaaat tttttcgtgc aaacgaaata atatatttgt
    17101 tctttcacaa gcctttgtta agatagaaaa tgtctgtgaa tttaattccg taatatgaat
    17161 agatacatgt ctcttttttt gttcggttcg cttcaaggct ttttcaatcc aatcttgttt
    17221 ggcgttgaca aaatgtagcc ctttatctat atttacaccc aaaggaataa ttaattttat
    17281 tccattatca cccacctgaa cacgtataga acgagccttt gggctatagt aaatcaaaat
    17341 tttgcctaag tctttgtgta tataagtttg tgtcatgttt tagagcttaa gtactttcac
    17401 atgcaaatgt agaaaaaaaa gctaatattt tggcagtaaa agaattattc gtatatttgc
    17461 aaccgaaaaa gagataggtg aggggggcgg tagttcccct catttgttca cataaagtaa
    17521 ggtatgatat ctaaagacgt tgttgttcag actgttaatc aatttttgaa agataaagaa
    17581 gattatttct tagtggatgt tacaataagc atacataaca ctatttgtgt cgaaatagat
    17641 gcttttcaag gtgtagatgt agaaacatgc tgtgaactca gtcgttttat tgaaacaaaa
    17701 attgaccgag agatagaaga ttttgaatta gaagtaggtt ctgctgggat aacagcacct
    17761 tttaaaatac ttcagcaata tatcaaaaat atcaacaacg aagttgaagt tttattgaaa
    17821 aacgggaaaa aaataattgg atttttgcga agtgccaatg agcatacatt tgtggttgaa
    17881 gtgtcgcaaa aaatcaaaaa agataaaaat gccaaaaaag aagaagtaac ggagttacta
    17941 acttatgcgt attcagacgt taaacataca aaatataatc tcagatttaa ataacaatta
    18001 tgagcaacaa accagtacaa tcactgaatt tgatagaaac attttcagag tttaaagaat
    18061 taaaaaatat tgataaagcc acgatgatta gtgtgcttga agattctttt cgtagtgtaa
    18121 tagcgaaaat gtttggaacc gatgagagtt ttacagtcgt aattaatcct gataaaggtg
    18181 attgtgaaat ttggcgagac tatgaagtag tagccgatgg agaagtagaa gatgaggttt
    18241 tacaaatggg cttgtcaaaa gctcgtgcca ttgatgacga atgcgaaata ggtgatgatg
    18301 ttacagaggc agttaacttt ttgagtttcg gaagacgtgc tgtattaact ttgcgtcaaa
    18361 cattggcttc aaaaatctta gaattacaaa aagaatctgt atatgatcaa tacaaagaaa
    18421 tggttggtca agttgtttat ggcgaagtgt accaaatttg gaaaaaagaa atcttgttgt
    18481 tagatgatca aggcaacgag ttattattac caaaaacaga acaaatacct actgattttt
    18541 ataaaaaagg agatacggta aagtctgttg tagtgagagt tgataataaa aataatcagc
    18601 tgaaaattat agtgtctcgt acagctccaa tatttttaca acgtttattt gaacaggaaa
    18661 tacctgaagt gcaagaaggt actattatga tacgccgaat cgctcgtata cctggcgaaa
    18721 gagcaaaagt agccgtagaa tcatatgatg atagaattga ccctgttggt tcttgcgtag
    18781 gagtaaaagg ttctcgcatc cacagcattg ttagggaatt gagaaatgaa aatattgatg
    18841 ttattaatta tacatctaat ttatctttat ttattgcacg ttcattaagt cctgcaaaag
    18901 tgctttcgat tcgcatagac gaagaaagaa aacgtgcaga agtgtcaatt aaaccagaag
    18961 atgtgtctat ggctattggc aaaggaggat ccaatataaa attagctaca atgcttacag
    19021 aatacactat cgaagtattc agacatgtgg atgaggaaga cgaagatgat gtgttcttag
    19081 atgcattctc tgacgaaatt gaaccatggg ttatagatgt ttttaaatct atcggatgtg
    19141 atacagcaaa aagcgtatta gaaattcctc gtcaagaatt gattgataga actgatttag
    19201 aagaagaaac aatagatgaa gcattggcaa tactaaggca agaatttgaa taataggtca
    19261 ttttttgcaa aaggattagg aaaagatgag cattgccaaa aagaaaattt aattagaaga
    19321 aaacaaggag tatttatatg tcgacaaaat taaataaagt agtaaaagaa ttaaatgtag
    19381 gattagctac ggctgtagag tttttacata aaaaaggctt tgcggatgtg gaatctaatc
    19441 ccaatgctcg tataaccgaa gagcaatatg atttgctcgt caaagaattt agcaaagata
    19501 aaagccaaaa aattgaatct ggtattctcg cccaacgaag taaagaacga ccaaaagggg
    19561 aagttgttgc tctcgaaggt tttgataaag aaaacaaacc agtagaagaa attaaggtgg
    19621 aaattcaaga agcatctcgt cctcaattta aacaagtggg caagattgat ttagatgctc
    19681 ttgtgcctaa aaagaaagat acgaatgagg ttaaagaaga aatgcctaat atagatgttc
    19741 ctttgcaaga aaatgtcgaa aaaatagtaa cagtccagca agtagaacaa gctcctatac
    19801 aggaagtgga agaagaaata acagaacctt ctgtggtagt agaagctgaa atagaggata
    19861 aatcggaaac ggaaaatgta gtagaggata tagatgaatc ggttgatgat actatatctg
    19921 ctgaattaag tgatgatgta tatacgatag agaaaaatac cattcattcc acaatcaatg
    19981 tggttggaaa aattgattta gatgcgttaa atcaacaaac acgtccaaaa aagaaaacaa
    20041 aagaggaaaa gaaaaaagaa aggttagaac gcgaaattca aaaacctaca ccaacagtag
    20101 ctactgcacc aaaagatgcc gttcaaaaag aggcaacagt tgccgcagat aagaaaaaaa
    20161 gaagtcgtat tcaacgtgaa cgggtgaata tttatgaccc taatgcccaa gctaaaaaac
    20221 ctactaagcc tcaacctcaa gcaaataaca aagaggtaaa gaagtttgtt cctaaagagc
    20281 aaattagtga cgaagatatt caaaaacaaa taaaagaaac gctcgctcga ctcactaata
    20341 aaggaaaatc aaaaggagct aaataccgta aagacaaacg tgaagcagta agccaacgtc
    20401 tacaagaaga agctgattta gaaagattag aaagtcgcgt aataaaaatc acagagtttg
    20461 ttacagccaa tgagttggcg acaatgatgg atatgcctgt aacacaggtt attggtactt
    20521 gtatgagtat tggaattatg g
//
LOCUS       DAOSRP010000017        20079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2861_length_20079_cov_6.054135, whole genome shotgun sequence.
ACCESSION   DAOSRP010000017 DAOSRP010000000
VERSION     DAOSRP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20079
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2861_length_20079_cov_6.054135"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1237)
                     /locus_tag="PLJ82_02080"
     CDS             complement(41..1237)
                     /locus_tag="PLJ82_02080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR40330.1"
                     /translation="MSAVSPRLASLSESETLAMSQKSNELKAKGFDVINLSVGEPDFP
                     TPTHIKDAAKKAVDNNFSFYSPVPGYPALRNAIVEKLKKENGLQYTADQIVCSNGAKQ
                     SVCNVLLSIIGKGDEVIVPAPYWVSYVEMVKLAEGTNVIVSAGIEQDFKITPDQLESA
                     ITPKTKALILCSPSNPTGSVYSKAELGALAEVIARYPSIIVLSDEIYEHINYVGKHES
                     IAQFDAIRDRVVIINGVSKGYAMTGWRIGWIAAPKWIAAACNKLQGQYTSGPCSVAQK
                     AAEEAYIGNQECVADMRKAFERRKNLVFSLAKEIEGLQVNNPEGAFYVFPKCDSFFGK
                     KTPKGNVIADAGDLAMYLLEEAHVACVGGAAFGAPDCIRFSYAASDENLVKAFDRIKT
                     ALAELV"
     gene            1391..2278
                     /gene="era"
                     /locus_tag="PLJ82_02085"
     CDS             1391..2278
                     /gene="era"
                     /locus_tag="PLJ82_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOR40331.1"
                     /translation="MHKAGFVNIVGNPNVGKSTLMNALVGEKISIITSKSQTTRHRIM
                     GIVNGDDFQIVYSDTPGVLKPNYRLQESMLAFSQSALIDADVLLYVTDTVEMHDKNVD
                     FLNKIKGVDVPIFVIINKIDLLPNQEKLEELVALWQNTLPKAHIIPISATEKFNVDYV
                     LKQIKDLLPESPPYFEKDALTDRPARFFVTEIIREKILTNYDKEIPYSVEVVIEKFKE
                     TTKQIDISALIYVERDSQKGIIIGHGGKSIKKMGTEARLDIEKFFDKKVFIEFFVKVE
                     KDWRNKDKQLKDFGYYLNS"
     gene            2309..3625
                     /gene="der"
                     /locus_tag="PLJ82_02090"
     CDS             2309..3625
                     /gene="der"
                     /locus_tag="PLJ82_02090"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR40332.1"
                     /translation="MGSIVAIIGRPNVGKSTLFNRLTESRQAIVNEVAGTTRDRQYGK
                     SEWGGREFSVIDTGGWTFKSEDTFEEEIKKQVDIAIAEADVLLFVVDGQNGVTDLDME
                     VAQLLRKCKKPIVLAVNKTDNNELQYMAADFYKLGIGEPLCISAISGFGSGDLLDVIL
                     PLLPKKEDAPVYDEELPRIAIVGRPNAGKSSIINAFIGEERTIVTDIAGTTRDSIYSR
                     YNKFGKDFYLVDTAGIRKKGKVHEDLEYYSVIRSIKAIENSDVCVLMIDASRGVESQD
                     LNIFSIIQKNRKSLVVCVNKWDLVENKETNTSKEFEQTIRDRFAPFVDFPIIFASALT
                     KQRIFKVLETATEVYENKTQHISTSVLNDFFLPLIENYPPPALKGKYVKIKYVTQIRE
                     AKTPTFIFFANLPQYVKDPYKRFLENKIRANWNFHGNPIQVFIRKK"
     gene            3910..5088
                     /locus_tag="PLJ82_02095"
     CDS             3910..5088
                     /locus_tag="PLJ82_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR40333.1"
                     /translation="MKKYLTVFAAFIIMLCLGSVYAWSVIASELMSVYHFSAAQSQII
                     FGTIIAIFPITMIFVGQLARKLHPRYLGYISGVLFLSGYLLAAHANGHFFLTYLGLGI
                     TAGIATGFGYWVSLTIPVQWFPEKKGLITGVAAAGFGLGAVFISAIAEKMLIAGKDVL
                     EVVQFIGIVYGLTVILFSNLITPNSDLSKSGVVAIRIKECISSTIFLKLFSGIFLGTF
                     AGLLIIGSLKIIGEQSNINNHILILGVSVFAASNFLGRLIWGFLSDYIGASLSIFLAL
                     LIQSLAILSLNLFSLSDHTYLLLSFLIGFGFGGNFVLFAKEAAQLYGVQKLGLIYPYV
                     LLGYAIAGIAGPAVGGILFDFSDSFFYAILVASIMSLIGSVLFLKQYINARRNTLAKN
                     "
     gene            complement(5122..5460)
                     /locus_tag="PLJ82_02100"
     CDS             complement(5122..5460)
                     /locus_tag="PLJ82_02100"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HOR40334.1"
                     /translation="MKAVDYFHKKPALLNCAQAILTAWQKEYDIAENTILEFKQYGGG
                     RAPEGACGALYAAEYLMKKHHQQSIYKEFSVYAGSIACKEIRKNGVVSCQKCVEIADE
                     LLQNARVKKE"
     gene            complement(5536..7665)
                     /gene="pnp"
                     /locus_tag="PLJ82_02105"
     CDS             complement(5536..7665)
                     /gene="pnp"
                     /locus_tag="PLJ82_02105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR40335.1"
                     /translation="MMNVITKTITLGDGRTISIETGKLAKQADGSVMVRMGNTMLLAT
                     VCAAANAVPGTDFMPLSVDYKEKFAANGRFPGGFTRREGRASDYEILVSRLVDRALRP
                     LFPDDFHAETFVNVTLFSADGEDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDG
                     QFVVNPTFSALKNADMDIMVAATMDNIMMVEGEMKEVSEDDLLQAMKVAHEAIKVHCQ
                     VQLDLMEAAGKTQKRVYNHEDNDEELKKDIWAKTYDAVYAVASACNPDKHSRIANFEA
                     VKETYIASLTPEVAAEKKGLISKYYHDVEKEAMRRAILDEGKRLDGRKTTEIRSIWSE
                     IDYLPGAHGSAIFTRGETQSLTTVTLGTKQDEKLIDEALTQGKERFLLHYNFPPFSTG
                     EARASRGTGRREIGHGNLALRALKGMIPDNYPYVVRVVSEILESNGSSSMATVCAGSL
                     ALMDAGVQIKKPVSGIAMGLISENKGKNFAVLSDILGDEDHLGDMDFKVTGTRDGITA
                     TQMDIKVDGLSYEILEKALSQAKEGRMHILNKIEETIATPRPDFKANAPRIVTLQVPK
                     DMIGAIIGPGGKIIQAIQLESGASVSIEEKENVGVVEIAANNKQAIDIALAKVKGIVA
                     VPEVGEVYKAKVKSIMPYGAFVEFMPGKEGLLHISEISWERIEDMQLTGIKENDEIEV
                     KLLEVDKKTGKFKLSHKVLTQRPPRKE"
     gene            8361..10781
                     /locus_tag="PLJ82_02110"
     CDS             8361..10781
                     /locus_tag="PLJ82_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOR40336.1"
                     /translation="MKEFFTTKFRTWGEWIKQFIKQLVEWRKYRYHRFKNLVWYKKLA
                     NATATSFGLFLLLLLLIDINFLWLFGSSPSLYSISHPDQNIASEIISADKKVIGKYFT
                     ENRIPVTYNEISPILIKTLIATEDERFYEHFGIDFQGVMAAIKDMTKGNARGASTITQ
                     QLVKNMYKTRNQYSKGLFGYIPFVKLLIMKAKEWITAVKIEIFYSKEEILTMYFNTVD
                     FGSNAFGIHTAAKTYFKTTPDKLNYEQSATLVGLLKATTTYNPRLNPKNSTERRNVVL
                     QNLVSQGILTQIEADSLKAIPMKLDYSVEQSYDGNALHFRAFLSKYLEPWEKQTGYDI
                     YADGLKIYVTIDSRLQQYAEESVQKQMRTVQRRFNSHWGNENPWQDENRVEIPDFVEK
                     IARKTTTYSNLHKKYKGNKDSIDVYLNLKKKTKVFDYTYGEKDTLFSVVDSIRYMSKF
                     MHCSFMAMEPTSGEVKAWVGDINFPFWQYDKVGQSKRQPGSTFKLFVYTAAMMNGYTP
                     CDKLTDQQTIWNYVENGEPKTWIPRNAEVRYTGYNMTLKNAFARSINTIAVQLAQKIG
                     IPEVIKYAHLMGIKTPLENMPSTSLGSSDVSLLELVNAYATVINEGKYHEPILVTRIE
                     DRHGRVIYQANRTQKQVIPYETAFLMTEMLKGGMTERGGTTQALWEWDLFHYNTDFGG
                     KTGTSSNYSDAWFVGVTQNLIGGAWVGGEHRSIHFRTGNYGEGSRTALPVFALFMEKV
                     FKDPNFKHYQGKFPTKPKVKINKPYNCQSFVQPDSLFLSTDSLINDTLTDETFQPVNP
                     EELSPNDN"
     gene            complement(10827..11564)
                     /locus_tag="PLJ82_02115"
     CDS             complement(10827..11564)
                     /locus_tag="PLJ82_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432906.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOR40337.1"
                     /translation="MSSNKSIISVSIVAVSIILSVFILSNAFVNRNKTDNTISVTGLS
                     ERSFESDLIVWRSNYSVKNSRLTDAYAGLKNQTAKIADFLSKKGIESKDITFSAVDIT
                     KDYQYIYNQNGTSQSIFDGYTLTQTVSISSTQVTKIETISREITELINQGIEINSMPP
                     QYYYTKLADLKIAMLAEATEDGKLRATTIAENGGARLGNLKNSTMGVFQIVALNSSEE
                     YSWGGSFNTSSKYKTATVTVRLQYNIK"
     gene            complement(11678..14962)
                     /gene="mfd"
                     /locus_tag="PLJ82_02120"
     CDS             complement(11678..14962)
                     /gene="mfd"
                     /locus_tag="PLJ82_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOR40338.1"
                     /translation="MDIKEFSQSLTSHRVVNGINQWVESSHKNVCIQGFCGSSCALFF
                     ASSILAKTNTVYILNDAEEAAYFYHDLLQLLSPDDVYYFPSSYKKRVREGDQKDAANQ
                     VLRTEALNDISQKQSWILVTYPEAIAERVIPQQDLSKETLHLAVGEKLDSDFVISLLV
                     EYGFERVDFVYEPGQFAVRGSIIDVFSFSHDVPFRIDFFGDTIDTIRTFDIESQLSIE
                     KKTHISIVSNSVNIEENALVPLFKFFTNKVVYTIKNTPYVVDLIDKINQENDETRFIL
                     STEWIEQWQGNKVIEFGNSPFFSSPHIITLNQNIQPIFHKNFDLLAGSLTEYIAQQYQ
                     IYVLSDNPKQTERLKDIFEDKNFPITFTPINKTIHEGFVDNDLKICVFTDHQIFDRYH
                     KYSLKSQKTKAGKLVLSLKELMQFQVGDYVVHSEHGVGRFGGLIRTEINGKTQEVIKL
                     LYKDDDIIFVNIHSLHRISKYKSKEGEPPKINKLGTTAWVNLKEKTKKKVKDIARDLI
                     ALYAKRKSEKGFAFSPDSYLQNELEASFLYEDTPDQLKTTIALKKDMESDKPMDRLIC
                     GDVGFGKTELAIRAAAKAVADSKQVAVLVPTTVLAFQHFNTFKKRLKKFPCTVEYISR
                     ARKTSDVKLIIEKTKTGAVDILIGTHKLLSKELEFKDLGLLIIDEEQKFGVSTKEKLR
                     QLRSHLDTLTLTATPIPRTLQFSLMGARDLSVITTPPPNRYPVQTELIRFDDDVIREA
                     IEFELARNGQVFFVNNRVSNLPELEMQLKRLVPHARIAVGHGQMDSEKLERVLLDFIH
                     HEYDILLATTIIESGIDIPNVNTMFINNAQYFGLSDLHQLRGRVGRTNKKAFCYLISP
                     SLAILPTDARRRLQAIETLADLGSGIHIAMQDLDIRGAGNILGAEQSGFITDLGYETY
                     HRILDEAVSELKHEEFADVFAEEIEHHMNYVADCMFESDLELLFPSDYVQNISERMDL
                     YRRLDTINNEEDLQHFEKELIDRFGPLPFVSIELIQVVRLRWLCAALGIEKIQMKHGR
                     LTAYFISNPSSPYYQSETFGAMLLFAQTHPRNTQFREINGKRSLLLSGIDTIQKAYTI
                     FKDIRE"
     gene            15097..15834
                     /locus_tag="PLJ82_02125"
     CDS             15097..15834
                     /locus_tag="PLJ82_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOR40339.1"
                     /translation="MSDSLVLIPTYNEKENIENIIRAVFALPKSFHILVIDDNSPDGT
                     ADIVKRLQVEFSTQLHLLERKAKNGLGRAYIAGFNWALASAYEYIYEMDADFSHNPND
                     LLRLYEACSQGGADVAIGSRYVTGVNVVNWPMGRVLMSYFASKYVRFVTGLAIADTTA
                     GFKCYKRAVLETIELDKIKFVGYAFQIEMKFTAFKCGFNIKEVSVIFINRELGTSKMN
                     GKIFREAVIGVLQLRWESFFRKFPQKK"
     gene            15868..17211
                     /locus_tag="PLJ82_02130"
     CDS             15868..17211
                     /locus_tag="PLJ82_02130"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008021808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOR40340.1"
                     /translation="MLLISNGRIVNEGKIYKASILIKDGKIGAIYKDEVPKSIVEKVH
                     KIDADDCWVLPGVIDSHVHFRDPGLTHKGDLLTESKAAAAGGVTSIMDMPNTNPQTIT
                     VQAFEEKCQLASTKSLVNYSFYLGATNSNFEELQKIDPSKVCGIKLFMGSSTGNMLVD
                     KDSLLHRIFEDVKIPLAIHSEDEGTVKANAATYKAEYGENPIPMKYHAKIRSAEACYK
                     NTALAIELAKKYGTRLHILHLSTAKELALFDNVSLDKKNVTAEACPHYLLFDENDYDT
                     LGARIKCNPSIKSTQDKEALLAAINTQKIDTIGTDHAPHLLSEKEGDALTATSGMPQI
                     QHALVSMLELAKHGKTTIENVVEKMCHNPAKLYQINKRGYIRKGYYADIVIVQRNASW
                     QVNTDTILSKCGWSPYEGKKFSSKVTYTIINGNVVYSNGEFNEDTKGQALEFTRA"
     gene            complement(17219..18688)
                     /locus_tag="PLJ82_02135"
     CDS             complement(17219..18688)
                     /locus_tag="PLJ82_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOR40341.1"
                     /translation="MSLNIENILLIGSLLLLVSIIAGKTSYRFGVPTLILFLAVGMLS
                     GSEGLGGIRFDDPQIAQFIGIVSLNFILFSGGLDTHWHQVKPVLWQGITLSTVGVLIT
                     AISLGTFVWLITDFSLVESLLLGSIVSSTDAAAVFSILRSKSLSLKKNLRPTLELESG
                     SNDPMAYVLTIAFLTLVVHQDMSVVSIIPLFLQQMVFGAILGYVFGRISKLIINRIKL
                     DFEGLYPVLVIALMFLTFSATNFVGGNGFLAIYICAVYLGNQNLIHKRTILKVYDGLA
                     WLMQIVLFLTLGLLVFPSHILPVVGIGLLVSVFLIIIARPLSVFLSLLPFRMPIRSRF
                     YISWVGLRGAVPIVFATYPLLAGIDKADMIFNIVFFVSVSSVLLQGTTLGIVAKWLGV
                     ADKKKETTSSSSEVFKAEYPTASMQEIIIAPNAYAVNKTIAELSFPKSANIAMIHRDG
                     NYLTPTGSTVLAANDILVVLSNNEEGLSKAQACLSATSV"
     gene            complement(18749..19381)
                     /gene="nth"
                     /locus_tag="PLJ82_02140"
     CDS             complement(18749..19381)
                     /gene="nth"
                     /locus_tag="PLJ82_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR40342.1"
                     /translation="MLLKERYKGVIEWFQQHMPVAETELHYTNSFELLVAIVLSAQCT
                     DKRVNLTTPALFKSFPTPQAMAKASFEEVFALIKSISYPNNKAKHLIALSQQLVDKYD
                     GVIPDDAERLQELPGVGRKTANVMLVVAFKKPAMPVDTHVFRVANRIGLTTAKNPLQS
                     EKTLVKNIPNHLLPIAHHWLILHGRYTCLARKPKCDECGIRTFCKFFNKK"
     gene            complement(19393..>20079)
                     /locus_tag="PLJ82_02145"
     CDS             complement(19393..>20079)
                     /locus_tag="PLJ82_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOR40343.1"
                     /translation="KSSFGKITPFKNGAYLVIEHTEALHVIDVNSGNRSKAGNVQETN
                     ALEVNLAAVDEVARQLRLRDMGGIIVIDLIDMQENEHKQQVYERMKERMQNDSAKHNI
                     LPLSKFGLMQITRQRVRPQTLIDTKETCPTCDGNGKIRSSLFFVDMLETKLNEIKNTM
                     KIRKCILFVHPFVYSFINAGIMSIKNKWILKFGLGFKVLPNQSYSYLQYGFFDTDGNE
                     IDVKDTADMA"
BASE COUNT         6665 a         3745 c         3367 g         6302 t
ORIGIN      
        1 ctgttgctgg gttttcttcc actgaatgct aacagtaata ttacactaac tcagctaagg
       61 ctgttttgat acgatcaaaa gcttttacta aattttcatc cgacgctgca tacgaaaaac
      121 gaatacagtc tggagctcca aaagcggcac cacccacaca ggcaacgtga gcctcttcca
      181 acaaatacat cgctaaatct cccgcatcag caataacatt tcccttgggt gttttttttc
      241 caaaaaaaga atcacattta ggaaaaacat aaaatgcccc ttctgggttg tttacttgta
      301 agccttctat ttcttttgct aaagagaata ctaaattctt tcttcgttca aaggctttac
      361 gcatatctgc tacgcattct tgattaccaa tatatgcttc ttctgcagct ttttgggcta
      421 ccgaacaagg tccagaagta tattgtcctt gcagtttatt gcatgctgct gctatccatt
      481 taggtgctgc tatccaacca atacgccagc cagtcatagc atacccttta gatactccgt
      541 taataataac aaccctatcg cgtattgcat caaattgggc tatgctttcg tgtttgccaa
      601 catagttgat atgctcataa atctcgtctg acaatacaat tattgaagga tatcgtgcga
      661 taacctcagc caaagcacct aattctgctt tagaatacac actgcctgta ggattagatg
      721 gagaacacaa tatcaaggct ttggttttgg gtgttatagc cgactctaat tgatctggag
      781 taattttaaa gtcttgttca attccagcag aaacaatcac gtttgtgcct tctgccaatt
      841 ttaccatctc tacataactt acccagtaag gagctggcac aattacctca tctcccttac
      901 caataatact caacaataca ttacaaacag attgtttggc tccattggag cacacaattt
      961 gatctgccgt atactgtaaa ccattttcct tttttaattt ttctacaata gcatttcgca
     1021 aagctgggta tccaggcact ggcgaataaa acgagaaatt gttatctact gcctttttag
     1081 cagcgtcttt gatatgtgta ggcgttggaa aatcgggctc tcctacactt aaattaatta
     1141 catcaaaccc tttggctttt aattcattgc ttttttgaga catggctagc gtttccgatt
     1201 cggacaaact tgctaatcgt ggagatacag cactcatttt tctgatttat tgtgatttat
     1261 acttgcgttt tttactttgc gaacgaacac aaaatagccc acaaaaatac ataattgtta
     1321 tacaacatac aaggaattta tcggtttccg aagaaaaaag cgtatctttg cagtcataat
     1381 ttcaactttt atgcataaag caggttttgt aaatattgta ggtaacccca acgttgggaa
     1441 atcaaccctt atgaatgcct tagtcggtga aaagatttcg attataacat cgaaatcaca
     1501 aactacgcgt catcgcatta tgggtattgt aaatggagat gactttcaga ttgtatattc
     1561 tgatacacct ggcgtgttaa aaccaaacta tcgactacaa gaatctatgt tggctttctc
     1621 ccaatcggct ttgattgacg ctgatgtact tttgtacgtt accgacacag tagaaatgca
     1681 cgataaaaac gtggattttc tcaacaaaat taaaggtgta gatgtgccaa tctttgttat
     1741 tattaataag atagacttgt tgccaaacca agaaaaacta gaagaactgg ttgctttatg
     1801 gcaaaacaca ttacccaaag cacacataat ccccatctcg gcaacggaaa aatttaatgt
     1861 ggattacgtg ctaaaacaaa ttaaagattt attgccagag tcacctccgt attttgaaaa
     1921 agatgcctta accgaccgac ctgcacggtt ttttgtaacc gaaattatac gagaaaaaat
     1981 cttaaccaat tacgacaaag aaatacctta ttcggtagaa gttgtaattg agaaatttaa
     2041 agaaaccaca aaacaaatcg acatcagtgc tttgatatac gtagaaagag attcgcaaaa
     2101 aggcataatt attgggcatg gaggaaagtc gataaaaaaa atgggcacag aggctcgttt
     2161 agatattgaa aagttttttg acaaaaaagt atttattgaa ttctttgtta aagtagaaaa
     2221 agactggcga aacaaagata aacagctaaa agattttggt tattacctca attcttagtc
     2281 agcaacatta ttaatttata aaaataaaat gggaagtata gtagccatta ttgggcgacc
     2341 aaatgtagga aaatcaactt tattcaatcg tttaacagaa agccgtcaag ctatcgtaaa
     2401 tgaggttgcg ggcactacgc gcgaccgcca gtacggaaaa tcagaatggg gaggacgcga
     2461 attttctgta atcgatacag gaggatggac atttaaatcg gaagacacgt ttgaagaaga
     2521 aattaaaaaa caagttgata ttgctattgc cgaagcagat gtgttgttgt ttgtagtaga
     2581 tggacagaat ggggttacag accttgatat ggaagtagca caattgctcc gtaaatgcaa
     2641 aaaaccaatt gttttagctg ttaacaaaac agacaataat gaattacaat atatggctgc
     2701 tgatttctac aagctaggta ttggagaacc tctttgtatc tctgctatca gtggttttgg
     2761 atcgggcgat ttactggatg ttattttgcc tttattgcca aaaaaagaag atgctcctgt
     2821 gtatgatgaa gaattgcctc gtattgccat agtaggacgc cccaatgcag gaaaatcgtc
     2881 gattatcaat gcttttattg gcgaagaaag aacaattgta acggatattg caggcactac
     2941 acgcgattcg atatactcga gatacaacaa gtttggaaaa gatttttatt tagtagatac
     3001 tgctggcata cgaaaaaaag gaaaagttca cgaagatttg gaatattatt cggttattcg
     3061 ctctattaaa gccatcgaaa actcagatgt atgcgtgtta atgattgatg cttctagagg
     3121 cgtagaaagt caggacttaa atatattctc catcatacaa aaaaaccgca aaagcttggt
     3181 tgtatgtgtc aacaaatggg atttagttga aaacaaggaa acgaatacga gcaaagaatt
     3241 tgaacaaacc attcgggata gatttgcccc ttttgtggat ttccctatta tttttgcatc
     3301 agcccttacc aaacaacgga tttttaaagt gcttgaaaca gctaccgaag tatatgagaa
     3361 taaaacacag catatttcaa ccagtgtgct caatgatttt ttcttacctc taatcgaaaa
     3421 ttatccaccg ccagctctta aaggtaaata tgtaaaaatt aaatatgtaa cacaaatacg
     3481 tgaagcaaaa acacctacgt ttatcttttt tgcaaattta ccgcaatacg taaaagaccc
     3541 ttacaaacgt tttttggaga ataaaattag agccaattgg aattttcatg gcaacccaat
     3601 tcaagtattt atcagaaaga agtaagtcca atgacttact tctttctttt tataagggta
     3661 tgaatggatt atagaaagat taacaccaac gtatccaaac aaatgagtta ctaaaattac
     3721 ttaataaatg aagccacact ttatttatta agtggtaata tattatcttt gtatctaatt
     3781 taaaagaaac aacatataca ataactactt aaaaatggaa attatgatat tgatccaata
     3841 tgtttatttg gcactttgga taaaagaata catgcatcca tttatgcaac gattatacaa
     3901 aacaaatata tgaaaaaata tctaaccgta tttgctgcat ttatcatcat gctttgttta
     3961 gggagtgtat atgcatggag tgtaattgca tccgaattaa tgtctgtgta tcatttctcg
     4021 gcagctcaat cacaaattat cttcggcacc ataattgcca tatttcctat cactatgatt
     4081 tttgttgggc aattggctcg taaactacac ccacggtatt tgggatatat ttctggagtt
     4141 ctttttttga gcggatacct attagccgct catgccaatg gtcatttttt ccttacttat
     4201 cttgggctag ggatcaccgc aggaattgca acaggttttg gatactgggt ttctctaacc
     4261 atccctgttc aatggtttcc tgaaaaaaag ggcttaatta caggcgtagc cgcagctggt
     4321 tttggattgg gggctgtatt tatctctgct attgccgaaa aaatgttgat agcagggaaa
     4381 gatgtgctag aggtagtgca atttattggt attgtatacg gcttaaccgt tattctattt
     4441 tcgaatctaa ttactccaaa tagtgattta tctaagtcgg gagtggttgc tatccgaata
     4501 aaagaatgta tatcatcaac gattttcctt aaactattta gcggtatttt tttaggaaca
     4561 tttgctggtc tgcttattat tgggagttta aaaattattg gcgaacaaag caacatcaac
     4621 aaccacatac taatacttgg tgtatccgta tttgcagcat ccaacttctt ggggcgtctt
     4681 atttggggat tcttaagtga ttatatcggg gcaagtctta gtattttttt agcattgctc
     4741 atacaatcat tggctatttt atccctcaat cttttttctt tatcagacca cacctatctg
     4801 ttattgtcat ttttaatagg ttttggattt ggaggtaatt ttgtactatt tgccaaagaa
     4861 gcagctcaac tatatggtgt tcaaaaactt gggttgatat acccttatgt tttgctagga
     4921 tatgccattg ctggtatagc tggacctgcg gttggtggca ttctgtttga tttttcagat
     4981 tcattttttt atgctatttt agtagcaagt atcatgagct taataggcag tgtgctcttt
     5041 ttaaaacaat acattaacgc gagaagaaat acattagcaa aaaactagtg taatcgttgc
     5101 tacaaaaatt gagagtgctg tttattcttt tttgacacgg gcattttgca acaattcatc
     5161 tgcaatttct acacattttt ggcaactcac tactccattt tttctaatct ctttgcatgc
     5221 aatagagcct gcatatactg aaaactcctt gtaaattgat tgttgatgat gttttttcat
     5281 taaatactca gcagcataca atgccccaca agcaccctca ggagcacgtc cgcctccata
     5341 ttgtttaaat tctaggattg tattctctgc aatgtcatac tctttttgcc acgcagttaa
     5401 tatggcttgt gcacaattaa gcaatgctgg ctttttatga aaataatcaa cggctttcat
     5461 ttcttttatc ttttatttaa aaaaagcacc acatcctgaa aagaagtggt gctttgattt
     5521 tgattgaaca atatactatt cttttcgagg aggtctttgt gtcaatactt tatgcgataa
     5581 tttaaattta cctgtttttt tatctacttc aagtaattta acctcaatct catcattttc
     5641 ttttattcca gttaactgca tatcttcgat acgctcccaa ctaatctcag aaatatgaag
     5701 caaaccttct ttgccaggca taaattcaac aaatgcacca taaggcataa tagattttac
     5761 ctttgctttg tacacttcac ctacctctgg aactgcaact atacccttta ctttagctaa
     5821 agcgatatca attgcttgtt tattattagc tgcaatttct acaacaccaa cattttcctt
     5881 ttcttcgata gatactgaag ctccagattc taattgaatt gcttgaatta tttttccacc
     5941 aggtcctata attgcaccaa tcatatcttt aggtacttgt aatgttacaa tacgaggagc
     6001 atttgcctta aagtcaggac gtggagtagc aatagtttct tcaattttat ttaagatgtg
     6061 cattctgcct tctttggctt gcgaaagagc tttctctaaa atttcgtacg acaaaccatc
     6121 cactttaatg tccatttgtg ttgcagtaat accatcgcgt gtaccagtta ccttaaagtc
     6181 catatcaccc aaatgatctt catctcctaa aatatcagaa agaacagcaa agttttttcc
     6241 tttattttca gaaatcaaac ccatagcaat accagatact ggttttttaa tttgcacacc
     6301 tgcatccatc aatgccaacg aacctgcaca tactgtagcc atagacgaag aaccgtttga
     6361 ttctaagatt tctgatacta cacgtactac atatggataa ttatctggaa tcatgccttt
     6421 taatgctctt aaagctagat ttccatgacc aatttcacgt cttcctgtac cacgagatgc
     6481 tcttgcttca ccagtagaga atggagggaa gttataatgc aatagaaaac gttctttgcc
     6541 ttgagttaag gcttcgtcaa ttaatttttc atcttgtttt gtcccaagag ttactgttgt
     6601 aagtgattgt gtttcgccac gagtaaaaat tgctgaacca tgagctcctg gtaaatagtc
     6661 tatttctgac caaatagaac gaatttctgt tgtttttctt ccgtccaaac gcttaccctc
     6721 atcaagaata gcacgacgca tagcttcttt ttctacatca tggtaatatt tgcttatcaa
     6781 acctttcttt tcagcggcta cttctggagt taaactagct atatacgttt cctttactgc
     6841 ttcaaaatta gcaatacgag aatgtttatc tggattacat gcactagcta ctgcatacac
     6901 tgcatcatag gtttttgccc aaatatcttt tttcaattct tcatcattat cttcgtggtt
     6961 atacacacgc ttttgtgttt ttccagcagc ttccatcaag tcaagttgta cttgacaatg
     7021 tactttaata gcttcgtgag caactttcat tgcttgcaat aaatcatctt cagatacttc
     7081 tttcatttca ccttctacca tcatgatatt atccatagta gcggcaacca taatatccat
     7141 atcggcattt ttcaatgcag aaaatgtagg atttactaca aactgaccat caatacgagc
     7201 tacacgaact tcagaaatgg gtccattaaa aggaatatca gatactgcta aggcagccga
     7261 agcggctaaa ccagcaagtg catcaggcat atcttctcca tcagcagaaa aaagggttac
     7321 attaacaaac gtttctgcat gaaaatcatc tggaaataat ggacgtaaag ccctgtcaac
     7381 caatcgggaa acaagtattt cataatcaga ggcacgacct tcacgacgtg taaaaccgcc
     7441 agggaaacga ccgtttgcag caaacttttc tttataatca acagaaaggg gcataaaatc
     7501 agttccagga actgcatttg cggcagcaca aactgtcgcc aataacatgg tattacccat
     7561 gcgtaccatc actgaaccat cggcttgctt tgccaatttt ccggtttcaa tgctaattgt
     7621 tcttccatca ccgagtgtga tagtttttgt aattacattc atcatactaa aaattacatg
     7681 ttgtttatat attcttaaaa tttcgacaca aagatacatt ttaatccttg aaaatgagtt
     7741 tatttttaag gatttgtgtt gcatatgggt caaaaaaagt tgtttttaat caaacagaat
     7801 atttttcaac atttactcat tcatgtgtta ttggtctctt tgaattatat cttcagacac
     7861 attggttact atattttaca acatacaagc atagttttgt gtaaaaaatt tggataacaa
     7921 acaaaaagag tgtatctttg aagtaccgta tcgcatttgg tgaaacttaa cactctttta
     7981 catactgaga ctattttcaa tttctaatgg cgtgctgcta ttggaaacaa gtaggcagat
     8041 tacaaaggaa attgagcact caaaacttta gatatcgaag tttgactcgt aaatagcggt
     8101 acggtttttt ttgttagcgt aatgctaact tttttatttc tacaaaacag aaaacactaa
     8161 ctatcaaaca attaccacaa ccccccccct caattcttct gaatacttgt tttacttatt
     8221 taagaacaag acaaatatat cggcaataaa attttgacag aaacattctt ttaaataact
     8281 ttgacgccac aatatgaagt aagtttttcc tacttaacac ctatattcat attgaaacca
     8341 atagtgcttt tttatatact atgaaagaat tttttacaac aaaatttcgt acttggggag
     8401 aatggattaa acaatttatt aaacaattgg ttgaatggcg taagtatcgt tatcatcgtt
     8461 ttaaaaatct agtttggtat aaaaaattag ccaatgcaac agcaacatct ttcggtcttt
     8521 ttctattatt actcttatta attgatatca attttttgtg gttgtttggg tcttctccaa
     8581 gtttgtatag cataagccac ccagaccaaa acattgcttc agaaattatt tctgctgaca
     8641 aaaaagtaat cggcaaatat tttaccgaaa atcgtattcc cgttacctat aatgaaattt
     8701 ctcctattct tataaaaacg cttattgcta cggaagatga acgcttttat gagcattttg
     8761 gcattgattt tcagggagta atggctgcta taaaagatat gacaaaagga aacgcacgag
     8821 gagctagtac cattacacaa caactagtaa aaaacatgta taaaacgcgt aatcaatatt
     8881 caaaaggatt attcggttat attccatttg ttaaattact gataatgaaa gccaaagaat
     8941 ggatcactgc ggttaaaata gaaatttttt attcgaaaga agagatacta acaatgtatt
     9001 ttaatacggt tgattttgga agtaatgctt ttggaatcca tacggctgca aaaacgtatt
     9061 ttaaaacaac tccagacaaa ctcaattacg aacaatcagc tactttagtt ggactattga
     9121 aagcaactac aacctataat cctcgattaa atcctaaaaa ttcaactgaa aggagaaatg
     9181 tggtattaca aaatcttgtt tcgcaaggta tccttacaca aatagaagca gattcattaa
     9241 aggctattcc tatgaaactt gattatagcg tagaacaatc atacgatggg aatgccttgc
     9301 attttagagc ttttttatct aaatatttag aaccgtggga aaaacaaacg ggctatgaca
     9361 tctatgcaga tggcttaaaa atatatgtta ccattgattc gcgtctgcaa caatatgcag
     9421 aagaatccgt tcaaaagcaa atgcgaacag tgcaacggcg ttttaatagc cattggggca
     9481 acgaaaaccc ttggcaagat gaaaatagag tagaaatacc agactttgta gaaaagattg
     9541 cacgtaaaac aactacatac agcaatctgc ataaaaagta taaggggaat aaagactcca
     9601 tcgatgtata tttaaaccta aagaagaaaa cgaaagtatt cgactataca tatggagaaa
     9661 aagacacatt atttagtgtt gtggattcaa tacgctatat gagtaaattt atgcattgta
     9721 gttttatggc tatggagccc actagtggtg aagttaaagc atgggttgga gacatcaact
     9781 tcccattttg gcaatacgat aaagtaggac aatcaaaacg acaaccagga tcaacgttta
     9841 aattatttgt ttataccgct gctatgatga atggctacac tccttgtgac aaattaaccg
     9901 atcagcaaac catttggaat tatgtagaaa atggagaacc caaaacatgg attcctcgca
     9961 atgcagaagt taggtatact ggatacaaca tgactttgaa aaatgcgttt gctcgttcaa
    10021 tcaatactat agccgtacaa ctagctcaga aaattggcat tccagaagtg attaaatacg
    10081 cacatctaat gggaataaaa acccctttag aaaatatgcc atctacctca ttaggttcat
    10141 cagatgtatc tctacttgaa cttgtaaatg catatgccac agttattaat gaagggaaat
    10201 accacgaacc cattttagta acacgcatag aagaccgcca tggaagagta atttaccaag
    10261 ctaatcgtac acaaaaacag gttatcccct acgaaacagc ttttttgatg acagaaatgc
    10321 taaaaggtgg tatgacagaa agaggaggaa cgacacaagc cctatgggag tgggatttat
    10381 ttcactataa cactgatttt ggtggaaaaa ctggcacttc atctaactat tcagatgcgt
    10441 ggtttgtagg tgtaacgcaa aatttgattg gaggtgcatg ggtaggtggc gaacatagaa
    10501 gtattcattt ccgtactgga aattacggag aaggaagtcg cacagctttg ccagtgtttg
    10561 ctttgtttat ggaaaaagta tttaaagatc ccaattttaa acactatcaa ggcaagttcc
    10621 ctacaaagcc aaaagttaaa attaacaagc cttataattg ccaatcattt gtacaaccag
    10681 actcattatt tcttagcaca gactcattga ttaatgatac gcttacagat gaaacttttc
    10741 agcctgtaaa tccagaagaa ttaagcccaa atgataacta aacaaacaat tatcttcgtt
    10801 tagttatctt tgtttgtaaa agtgttttat ttaatattgt attgcaacct aaccgtaaca
    10861 gtggctgttt tatattttga tgaggtatta aacgatccgc cccagctata ttcctctgag
    10921 gaatttaatg ccacaatttg aaatacgccc atagtagaat tctttaaatt acccaaacga
    10981 gcaccaccat tctcagcaat agttgtagca cgaagttttc catcttctgt tgcttcagcc
    11041 aacatagcaa tttttaaatc ggccaatttt gtataataat attgaggagg cattgagtta
    11101 atttcaatcc cttgattgat taattcggta atttcgcgag aaatagtttc aatttttgta
    11161 acctgagtag aagaaatact tactgtttgt gtaagagtat aaccatcaaa aatagattgt
    11221 gatgtgccat tttgattgta tatatattga taatctttag taatatcaac cgctgaaaaa
    11281 gtaatatcct tactttcaat tccttttttg gataaaaaat cagctatttt agctgtttga
    11341 tttttcaacc ctgcataagc atccgttaaa cgagaatttt tcacggaata gttacttcgc
    11401 caaacgatta agtctgattc aaaactacgt tcagataaac ccgttacaga tattgtatta
    11461 tctgttttat tgcgattaac aaacgcgttg gataaaataa acacggataa aataatgctt
    11521 acagcaacaa tacttactga aataattgat ttattactac tcataattta aggattttac
    11581 tttttattac aaatataaag gtaatatttt gagtaaagat acatttatgt ttatttttaa
    11641 aagggataat gtaaaaaagg caacaataga ctttacttta ttctcgaata tctttaaaaa
    11701 tagtatatgc tttttgtatc gtatctatac cagacagcaa cagagagcgt ttcccgttta
    11761 tttctctaaa ctgggtattg cgtggatgcg tttgagcaaa taacaacata gcaccaaaag
    11821 tttctgattg ataatagggg gaagatggat tagagataaa ataagcagtc aatctaccgt
    11881 gcttcatttg aattttctca atacccaatg cagcacacaa ccaacgtaaa cgcactactt
    11941 gtatcaattc aatagagaca aatggcaacg gaccaaaacg gtcgatcaat tctttctcaa
    12001 aatgttgcaa atcttcttcg ttgtttatcg tatctaatcg gcgatacaaa tccatacgtt
    12061 cagatatatt ctgcacataa tccgaaggaa acaacaactc aagatcagat tcaaacatac
    12121 aatcagccac ataattcatg tgatgttcta tttcttctgc aaacacatca gcaaactctt
    12181 cgtgctttaa ctcactcact gcttcatcta atatgcgatg gtatgtttca taccctaagt
    12241 ctgttataaa accactctgt tctgctccca aaatatttcc agctccacga atatccaaat
    12301 cttgcatggc aatatgaatg ccactaccta aatctgccaa ggtttcaatt gcctgcaagc
    12361 gtctgcgagc atctgtaggc aatatggcta aagaaggtga aattaggtaa caaaacgctt
    12421 ttttattcgt tctacctacc ctgcctcgta attggtgcaa atcactcaac ccaaaatact
    12481 gggcattatt aataaacatg gtatttacat ttgggatatc aataccagat tcgataatgg
    12541 tagttgctaa taaaatatca tattcgtgat gaataaaatc taacaaaact ctttctagtt
    12601 tctccgaatc catctgtcca tgacctacag ctatacgagc atgtggaacc aagcgtttaa
    12661 gttgcatttc caattcaggc agattggata ccctattgtt cacaaaaaat acttgtccgt
    12721 tgcgtgctaa ctcaaattca atggcttcgc ggataacatc atcgtcaaaa cgaataagct
    12781 ctgtttgtac aggatagcga ttgggcggtg gagttgtaat cactgacaaa tcacgagctc
    12841 ccatcaagga aaattgtaaa gtacgaggaa taggggttgc cgtaagagta agcgtatcaa
    12901 gatgagagcg taattgacgc agtttttctt ttgtcgaaac accaaatttc tgttcttcat
    12961 caattataag caatcctaaa tctttaaatt ctaactcttt actcaacaat ttatgagttc
    13021 caataagaat atcgactgca ccagtttttg ttttttcaat aattaatttt acatctgatg
    13081 tttttcgggc tctactaata tactcaacag tacacggaaa cttttttaag cgttttttaa
    13141 aggtattaaa atgctgaaat gccaatacag tagtgggcac caacacggca acttgtttgc
    13201 tatcggctac tgctttagcc gcagctctaa tcgctaattc tgtttttcca aaacctacat
    13261 caccacaaat aagcctatcc attggtttgt cgctttccat gtcttttttc aaggcaatag
    13321 tcgtcttgag ctgatcaggt gtatcttcgt ataaaaaaga tgcttcaagc tcgttttgca
    13381 aataactatc aggagaaaag gcaaaaccct tttcgctttt gcgtttcgca tacaaagcaa
    13441 taagatcacg agcaatatct tttacctttt tctttgtttt ctcttttaga ttaacccaag
    13501 ctgttgtacc taatttattt atctttggag gctctccttc tttgcttttg tatttggaaa
    13561 tacgatgcaa tgaatggata tttacaaaaa taatgtcatc atctttatac aaaagtttga
    13621 taacttcttg tgtttttcca ttaatttctg tacgaataag cccaccaaac ctacccacac
    13681 catgctcact atgaacaaca taatcgccaa cttgaaactg cattaattct ttgagcgata
    13741 aaaccaattt tcctgctttt gttttttgcg atttaagaga atatttatgg tatctatcaa
    13801 aaatttgatg atctgtaaaa acgcaaatct tcaaatcatt atctacaaat ccttcgtgaa
    13861 tggttttatt gatgggtgta aacgtgattg gaaaattttt atcttcaaaa atatctttta
    13921 atcgttctgt ttgtttggga ttatcactca atacatagat ttgatactgt tgtgctatat
    13981 actccgtcag actaccagca agtaagtcaa aatttttgtg aaatattggt tgaatattct
    14041 gattgagtgt aataatgtga ggagaagaaa agaaaggtga atttccaaac tcaattactt
    14101 tattcccttg ccattgttca atccattctg tagataaaat aaatcgggtt tcgtcatttt
    14161 cttggtttat cttatcaatt aagtctacta cgtatggggt gttttttata gtatagacga
    14221 ccttattcgt gaaaaatttg aataaaggaa ctaaggcatt ttcttctata tttacgctat
    14281 ttgatacgat tgagatatgc gttttctttt cgattgacaa ttgactttca atatcaaagg
    14341 tacgaatagt atcaatagta tctccaaaaa aatcaattct aaaagggaca tcatgtgaaa
    14401 aagaaaacac atctataata ctaccacgca cagcaaattg ccctggttca tatacaaaat
    14461 ctacgcgttc aaaaccatat tcaacaagca atgaaataac aaaatcgcta tccaattttt
    14521 ctcccacagc caaatgaagc gtctcttttg acaaatcttg ttgaggaatt actctttcag
    14581 caatagcttc aggataagta acaagtatcc aagactgttt ttgagaaata tcgtttaaag
    14641 cttcagtacg caacacttga ttagcagcat ctttttggtc tccttcacgc actcgttttt
    14701 tgtacgatga aggaaaataa tacacatcat caggggataa taattgcaat aagtcgtgat
    14761 aaaaatacgc agcctcctca gcatcattta ggatatatac cgtatttgtt ttggctaata
    14821 ttgaagatgc aaaaaaaaga gcacacgaag atccacaaaa accttgtata catacgtttt
    14881 tgtgtgaaga ctcaacccac tgatttatgc catttacaac ccgatgtgag gtgagtgatt
    14941 gcgaaaattc tttaatatcc actgaaaagt ttttgcaaaa gtacacaatt ttaggttaaa
    15001 gattaaaaaa tgaagacaaa atctcaattt atttacaatc ctttatgtat ctttgggaac
    15061 aaattaaata accactcaaa ttcagtaaat actatcatgt cggacagcct tgttcttatc
    15121 ccaacctaca acgaaaaaga aaatattgag aatattattc gtgcagtttt tgctttacca
    15181 aaatcgtttc acattttagt cattgatgac aactctcctg atggtacggc agatattgta
    15241 aaacggttac aagtagagtt ctctactcaa ttacatttac ttgagcgaaa agccaaaaat
    15301 ggtttaggta gagcatatat tgctggattt aactgggctt tggcttctgc ttacgagtat
    15361 atatatgaaa tggatgcaga tttctctcat aatcctaacg atttattacg actgtacgaa
    15421 gcctgctctc aaggaggtgc agatgtagcg ataggttctc gatacgtaac tggagtaaat
    15481 gtagtaaact ggcccatggg ccgtgtattg atgtcgtatt ttgcatcaaa atatgtacgt
    15541 tttgttaccg gcttagccat tgcagatact acagcaggct ttaaatgtta caaaagagct
    15601 gtgctcgaaa ctattgaatt ggataaaata aaatttgttg gatatgcttt tcaaattgaa
    15661 atgaaattta ctgcttttaa atgcggattc aatatcaaag aagtatctgt catttttatc
    15721 aatcgcgaat taggaacatc aaaaatgaat ggtaaaatat ttagagaggc tgtaatcgga
    15781 gtattacaac tacggtggga aagcttcttt agaaaatttc cacaaaaaaa ataatcaccc
    15841 acaaatagaa cattttatat tgacattatg ttacttatca gcaacggtcg cattgtaaac
    15901 gaaggaaaaa tttacaaagc atctattctt ataaaagatg gcaaaattgg tgccatttat
    15961 aaagacgaag ttccaaaaag tatagttgaa aaagtccaca aaattgatgc agatgactgc
    16021 tgggtattac ctggagtgat tgatagtcat gtgcatttta gagaccctgg acttactcac
    16081 aaaggagatt tacttaccga gagtaaagct gcagcggcgg gtggtgttac atctattatg
    16141 gacatgccaa acactaatcc tcaaacaatt accgtacaag cctttgaaga aaaatgccaa
    16201 ttagcatcta ctaaatcatt ggtaaactac tctttttact taggtgctac taattccaat
    16261 ttcgaagaat tacaaaagat tgacccctcc aaagtgtgtg gcataaaatt atttatgggt
    16321 tcatcaactg gaaatatgct agtagataaa gattcattat tgcatcgtat cttcgaagat
    16381 gtgaaaatcc cattagctat tcatagtgaa gacgaaggaa ccgttaaagc aaatgctgcc
    16441 acttataaag ctgaatatgg agaaaatccg attccaatga agtaccatgc aaagattcgt
    16501 tcagctgagg catgttacaa aaacacagct cttgccattg aattggcaaa aaaatatggt
    16561 actcgtttac acattttgca tcttagcact gccaaggaat tggcattatt tgataatgta
    16621 agtttagata aaaaaaatgt aacggctgaa gcttgtcctc attatctgct attcgacgag
    16681 aacgattatg ataccttggg tgctcgtatt aaatgtaacc catctatcaa atctactcaa
    16741 gacaaagaag ctttactggc tgctattaat acacaaaaaa tcgataccat tggcactgac
    16801 catgcccctc atttattatc cgaaaaagaa ggagatgctc ttacagctac atcaggcatg
    16861 ccccaaatac aacacgcact agtaagtatg ctcgaattag ctaaacatgg caaaactacc
    16921 atcgaaaatg tggtagaaaa gatgtgtcat aacccagcca aactctatca aataaacaaa
    16981 cgaggctata ttcgcaaagg gtattatgcc gacattgtta ttgtgcaacg caatgctagt
    17041 tggcaggtaa acacggatac tattttaagc aaatgtgggt ggtcgcccta cgaaggaaaa
    17101 aaattctcct ctaaggtaac ctacaccatt atcaatggga acgtagtata tagcaatgga
    17161 gaatttaacg aggataccaa agggcaagcg ttagaattta ctagggcata acaagacctt
    17221 ataccgatgt tgctgacaaa caggcttggg ctttgctcaa gccttcttca ttattcgaca
    17281 atacgaccaa tatatcatta gcagcaagta ctgttgaccc agtaggtgtt aaataatttc
    17341 catcacgatg aatcatggct atattcgcac ttttgggaaa agacaattca gcaattgttt
    17401 tattaacagc atacgcattg ggagcaataa taatttcttg catcgaagca gttggatact
    17461 ctgctttgaa aacctcagat gaagatgatg tggtttcctt ttttttgtca gctaccccca
    17521 accattttgc gactattccc aatgtagtcc cttgtagtaa tacagaagag accgatacaa
    17581 aaaagacaat attaaaaatc atatctgctt tatcaatacc tgccagtaag ggatatgtag
    17641 caaacacaat aggcacggca cctcgtaagc caacccaaga aatataaaaa cggctacgta
    17701 tgggcattct aaaaggaagc aagctaagaa aaacacttaa aggacgagct atgattatta
    17761 aaaatactga taccaacaat ccaataccaa caacaggtaa aatatgactt ggaaaaacta
    17821 atagtcctaa tgttaagaaa agcacaattt gcatcaacca cgccaatcca tcataaactt
    17881 ttaagatagt tcgcttatgt attaagtttt ggttgcctaa ataaacggca catatgtata
    17941 ttgcaaggaa accatttcca ccaacaaaat tagttgccga aaacgttaaa aacatcaggg
    18001 caatgactaa cactggatat aagccttcaa aatctaattt tatgcgatta ataataagtt
    18061 tgcttattcg tccgaaaaca taccccaaaa tagcaccaaa aaccatttgc tgtaaaaaca
    18121 gaggaataat agaaacaact gacatatctt gatgaacaac caaggttaaa aaagcaatgg
    18181 ttaaaacata agccataggg tcgttacttc cactttcaag ctcaagagta ggacgtaaat
    18241 tctttttaag agataaactt tttgacctca aaattgaaaa tacagcagct gcatcagttg
    18301 atgacacaat agaccccaac agcaaacttt ccactaacga aaaatcagta attagccaaa
    18361 caaatgtacc caacgatatg gctgtaatta aaactcctac agtcgaaagc gtgatacctt
    18421 gccacaaaac aggcttaacc tgatgccaat gggtatctaa cccgccagaa aaaagaataa
    18481 agtttaatga aacaatgcca ataaattgag caatctgagg gtcatcaaaa cgtatccctc
    18541 ctaatccctc agaaccagac aacatcccta cagctaaaaa taaaattaac gttggtacac
    18601 caaagcgata cgatgtttta cctgcaataa tacttactaa gagcaataac gacccaatta
    18661 acaggatatt ttctatattc aaactcatag attgcaggca taaaagtatg atgcctacaa
    18721 agatataaaa aatccgaaag aaaccttttt actttttgtt aaaaaactta caaaaagtac
    18781 gtatcccaca ttcgtcgcat ttgggtttgc gtgccaaaca ggtataacga ccatgcaaga
    18841 taagccaatg atgtgctata ggtaataagt ggttgggaat attctttact aaagtctttt
    18901 cactttgcaa tgggtttttt gcagtagtta aaccaattct atttgctacc ctaaatacat
    18961 gtgtatctac aggcatagct ggcttcttaa aagctactac gagcatcaca ttggctgttt
    19021 tgcgacccac accaggcaac tcttgcaaac gctcggcatc gtctggtata actccatcgt
    19081 atttatctac taattgctga gataaagcta ttagatgttt tgctttgtta tttggatatg
    19141 aaatactttt gataagagca aaaacctctt caaaagaagc tttagccata gcttgtggag
    19201 taggaaaaga cttaaataaa gcaggagttg ttaaatttac ccgtttatct gtacattgag
    19261 cagacaaaac aatagctact aataattcaa atgaatttgt atagtggagt tctgtttcag
    19321 cgacaggcat atgttgttga aaccactcaa taacaccctt ataacgctct ttaagtaaca
    19381 tacacaaaaa atttaagcca tatcagccgt atcttttacg tcaatttcat ttccatctgt
    19441 atcaaaaaag ccatattgca aataactata gctttgattg ggtaacactt taaaacctaa
    19501 gccaaacttg agtatccatt tattcttaat actcatgata cccgcattaa taaaactata
    19561 cacaaaagga tgtacaaaaa gaatacattt tcgtatcttc atagtattct taatttcatt
    19621 taattttgtc tccaacatat ccacaaaaaa cagggaggaa cggattttac catttccatc
    19681 acacgttgga caggtttctt tcgtatctat aagcgtctgt ggtcgaacac gttggcgtgt
    19741 aatttgcatt aaaccaaact tgcttaatgg taatatatta tgttttgcag aatcgttttg
    19801 catccgctcc ttcatgcgtt cgtacacttg ttgcttatgc tcgttttctt gcatatcaat
    19861 caagtcaata acaataatac ctcccatatc acgtaatcgt aattgccttg ctacctcatc
    19921 aacagcagct aaattaacct ctaacgcatt ggtttcctga acattaccag ctttagatcg
    19981 attcccacta tttacatcaa ttacatgcaa tgcctctgta tgctcaataa ccaaataggc
    20041 accgtttttg aaaggagtaa tcttaccgaa tgaggattt
//
LOCUS       DAOSRP010000018        19724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_2924_length_19724_cov_4.390259, whole genome shotgun sequence.
ACCESSION   DAOSRP010000018 DAOSRP010000000
VERSION     DAOSRP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19724
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_19724_cov_4.390259"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="prmA"
                     /locus_tag="PLJ82_02150"
     CDS             complement(<1..781)
                     /gene="prmA"
                     /locus_tag="PLJ82_02150"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOR40344.1"
                     /translation="MINYLRFAIKMNPFSEYLADILAYELGAIGFESFELQEDGVSAF
                     IQEPDWKGALFSEVIHDVAKLYETQISFKKERIVQQNWNEEWEKHYFKPILVDDVCLI
                     RSSFHTNLPNAKYDIVIDPKMSFGTGHHETTSLMLRWILEEVLVDKAVLDMGCGTAVL
                     AILAKMRGANKVWAVDIDEWCYVNAKENILVNAVDTIQVFQGGAEVVDNEKFDVIFAN
                     INLNVLLEYMPQYVACLSKNGILFMSGFYLSDLSHIHDLAIK"
     gene            937..1968
                     /locus_tag="PLJ82_02155"
     CDS             937..1968
                     /locus_tag="PLJ82_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40345.1"
                     /translation="MKKKALLISSLFIAFSMSMLAQYDDIYYNPRKANETRITTSNEE
                     TVVISNNVRDVDTYNRQAPANRPNQTSNVEQDDFEYTKRITRFHNPEAVTINNPEYVY
                     IYNDTPSEPNPTINIRVTLRWNPWDYSYFTRYYYPSYHWSWDSNYGWGYTYNTYYPFY
                     STYPSSFYYGYYPPGYGYYGYGYYGYPSYYHHRPYYYHHPYHHKPTYTTRKGYNNYDR
                     TPNYVNDGRGSSSSYGYQNSGQRSTRSVGTNPTNTNASSTRNTSTYNNRERTFNSAPS
                     SSTQNTRKSDVTTNTSTPRETSSGRSFNSSSSSSNSNNSGTRSTQRSDNSSSSNGGSR
                     SNGGRSFSR"
     gene            1998..3575
                     /locus_tag="PLJ82_02160"
     CDS             1998..3575
                     /locus_tag="PLJ82_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40346.1"
                     /translation="MKKIIITSLCALGIMSFANAQFQSEYDALKVAQTDLNGTARFMS
                     VAGAMGALGGDASTLFYNPAGIATYRSSELTFTGNWNWNNTSALGSMASRFLFNLNNV
                     SYISSYKTGSENGLVAFNWGFAYNRAKTFDKNIRYSTNSNLSLGDFLANYTEISGNAA
                     GYTASVFDEDNDVYRDQRVPWASVLAWDGMMIANPQDESNNSINNQYESYRNNLISQS
                     IGGGVQNTYTIAERGSVNEYAFSGGANISNILQLGVSFVLLNLDYQMESIFEEKFENN
                     STYKYTSSYQTDGSGFSIKVGAIARPTQWLRIGGAFHSPSWYYIKDNNEGSIGSKIVT
                     EVKPDYNGSTPVGYGSYYLNTPMKGLANLGFVIGNYGFVGVDYEYTDYSTMKMKNNDI
                     INSYMVDQNNKIATNFDATHTIRVGTELRPIENVSVRLGYAMVTPAIKADAYRTYATN
                     TTRTDFDFTNDKSTNYITAGLGYRFGKSYMDVAYVLQTKLQDYYTYNPTYVNGFGAPV
                     EITSKHNQILLTYGVRF"
     gene            complement(3651..4835)
                     /locus_tag="PLJ82_02165"
     CDS             complement(3651..4835)
                     /locus_tag="PLJ82_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOR40347.1"
                     /translation="MSLLSQKLAKYDAPQKIQEAGVYPYFRPISSDQDTVVQINGKPV
                     LMFGSNSYLGLTNHPEIKQAAIKAVEKYGTGCAGSRFLNGTLDIHLELEARLAKLVGK
                     EEALVYATGFTVNSGVVSCLTGKEDYVILDKANHASIIEGSRLSFSKIIKYAHNDMDA
                     LEKQLKKCEPDKVKLIVVDGVFSMEGDEAKLLEIADLAKKYNASIMVDEAHSLGVYGN
                     HGAGICNHLGVTKDVDLIMGTFSKSLATIGGFIAADKHIINYLKHTSRTLIFSASITP
                     ASTACVLAALDVMEKETWRMDALWANTNYMRAKLQKAGFDTGHSTTPIIPLYVRDNTK
                     TFMLTRMLMEDGVFVNPVVSPAVASEDTLIRISLMATHTFDQIDEAVEKITKNATLLA
                     IL"
     gene            complement(4927..5448)
                     /locus_tag="PLJ82_02170"
     CDS             complement(4927..5448)
                     /locus_tag="PLJ82_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HOR40348.1"
                     /translation="MIRKEEIFEAGKFHKTHGVKGELSCSFSFPVDSSKLSFIVSNIE
                     GIYVPFFIEDMREKTSTSLLLKLKNINTDEDAKILLDSPIYLPNELRKTDDEEVDVAY
                     FIHFTVVDANCGKIGAITAIDDSTPNTLFCVDTEQGETLYIPVAEEFFLSIDESTKTI
                     YMDLPTGLLTLNI"
     gene            complement(5455..11238)
                     /locus_tag="PLJ82_02175"
     CDS             complement(5455..11238)
                     /locus_tag="PLJ82_02175"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOR40349.1"
                     /translation="MKKIGIFITLLLSLTFVSFLLAKQQSVAMSYPTYWQQVDSLQTH
                     GMPKSANEIVQKIKQKALSEHAHEEIIRCLIYDLKTNVEIDHDSFFDQVAEIERYASS
                     SKDVISQSVLYAMLAQVYDSYFNANSYIIQQRTPIWDIVPENKKEWASNHFLYRIFHY
                     ASLSIQHEVALQKTLVTNYPTILVQATDSYLRPTVYDVLAHNYIQLYVQNKHQIERLV
                     TQEKSTEMSLITPYDHFIAEADTTTSLWQVVPRVKNVYQKLLTFHKANGNTDALVKTD
                     IDRIETLIALSIPSEKVSESYQLTLNSIIEKFQKSPIVVHAIVLKANYLLSENKRKEA
                     YDLCKLYINRFPDYSRIAVLHNIVEQIKQPQLATSSQSVVYPDSSLNLKLNFSNIDSL
                     TIRLYKIRSNSLEYQIVKNYYGITRQAIDRELVNTIHVTLPVSELFLSKDTSIHIKTK
                     DLGLYEYEIVSPTNAFDSIKNTFYVTRLLLVHKSINKTIEGWVVDRITGKPIKNATVT
                     VHQVRYQRNNVDVKKVASTNTNKNGLATFSLQHNNAYYLSVSQGEDTSTPIGNVYVYG
                     KQKENTPPDMQQHIALFTDRALYRPAQSVYVKGIAWNVKEDSVILEKNKKIHIRLRDA
                     NYQIIAEKQVQTSSYGSFNTDFILPKSGLTGSFTLETENGSSSFLVEEYKKPTFKAEI
                     KRIDKEVLFNNPVRINGNAETYMGFPLQAAHGKYRIVRQQFSWRRYFPIHTTENVAEG
                     VFKTNDKGEFTFDFIPIKPDTDQSAYYHYLVYVDITDKAGETQQVSSSFSIGESAIYI
                     QTNIPAIIQKEKTTPIAVNVTNVNGEMLHQSGKIAIYALKESSNNDTPVDSLQLSKCV
                     YEGSFVSGKPFSLNIVSNLPSGRYKLISSTKTQSLTEVENIHFFTLFSVKDKKPPFKT
                     SNWMYALSDTCSVGEMASTIIGSSANVLVMCELVGEDGLICRQFITLNNESKHIQFPY
                     TKNCGNSVEMRFTSVVNNTLYQQSNRIVKRQPSKKISIQYITFRDKLQPGNQEEWQIH
                     VKDANGVNQQAEVLVCMYDASLDAIVPHQWQFNPFVSHQWYVPSWNATPSFSRSNYAT
                     GIVPFKDEKGFELPKINLFDFQYPGYCRYAMPYMAVGGNMLRKSLEVIDDVMDEVVED
                     KVLSTETTASASSQLSQKMPIRKEFAETAFFYPAIKTDNEGLATVRFTVPESLTTWKI
                     MTLVHNADAYYGQQTSYVYTQKAISVKPNLPRFVQQSDSIVLTATIYNMLDSLTKTTA
                     KWEIRDAQTDSILLSNEEKVTLNPHQSVVIKLPLFVPTNSSLWVVKVMVANDSYSDGE
                     QHYLPILSSKKLVTESMPFTMYKEGEYTFEFDKLNNNTSTTLSHKRYTIELSPNVAWY
                     ALQALPVVAEPEYDNAISLMSSYYANMLGEFVVANTPLFKQYIQLYANKEESPLVKNE
                     ELKNLFNSESPWIQDALQETEGLSKLQLYLDTNQLHHKQQELWKKLQELQMPTGGFSW
                     FAGMPENEMVTLFIAEKIAKLVSIQALDSTIFGTEMMQRAVAYIDYQIIKSYNTYIKH
                     STNKPFVVDAYTLQALYVRSLFSTIPKSEELNKSIIYFNAVLKQNWTAYSLYGKSQVA
                     RILFRNNEKKLAQSIVTSLLEFATENKDKGIFWANNTAGYTWYQMPIVTHVAIMEAIA
                     EIKPNKEIEDKLKIWLLTNKQTNAWNNPIATVDAIYALVFTGNSMSIVQPSSMIVKVN
                     NNVLFESGEESSIGYRKHTFYNSEISPSLSKLTVNKKGNNVSWGAIYWQYEEEVSKIT
                     KPSMHALNIDKKMFVEQQRGLKTILVPIENKQLQVGDKVIVRLTISTDRDMDFVHIKD
                     LRASGLEPVEQVSTYRYREGIGYYQNPKDTHTNFFFSQFPKGTYVIEYPLWVRFNGVY
                     SGGISTIQCLYAPEFQSHSLVQQLFVK"
     gene            11539..12390
                     /gene="hisG"
                     /locus_tag="PLJ82_02180"
     CDS             11539..12390
                     /gene="hisG"
                     /locus_tag="PLJ82_02180"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOR40350.1"
                     /translation="MLRIAVQAKGRLYEETMLMLSESSIKIESGKRTLLIPAKNFPIE
                     VLFLRDDDIPQAVASGIADVGVVGENEFAEKQEDAEIVKRLDFSKCRLSLAIPKSIDY
                     QGVHWFEGKKIATSYPVILENFLKKHNIQAEIHVITGSVEIAPGIGLADGIFDIVSSG
                     STLVSNNLKEVEVVMKSEAILIANKQLNNEKRAILEELLFRFNAYKNAEGKKYIILNI
                     SNDKIDAVCKLLPGMKSPTVTPLAEEGWSSLHSVIEEKTFWDIINQLKALGAEGILVV
                     PIEKMIL"
     gene            12412..13695
                     /gene="hisD"
                     /locus_tag="PLJ82_02185"
     CDS             12412..13695
                     /gene="hisD"
                     /locus_tag="PLJ82_02185"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOR40351.1"
                     /translation="MYYIQYPEKKEWASLLARPTFDTTNLFDTVNHILVDVQKRGDKA
                     VIEYEEKFDKIHLDVLQVSDNELASAETKISKELKDAILIAKKNIANFHAAQVYSDVT
                     IETMPGIHCWQKNLPIEKVGLYIPGGTAPLFSTVLMLAIPAQIAGCKEIILCSPPNSN
                     GDIHPAILFAAHISGVSKVYKIGGVQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     VSLKDVAIDMPAGPSEVEVIADETANPAFVAADLLSQAEHGADSSAILLTTSNELATA
                     VQQEIQQQLPLLSRAEIAVKALNNSKIILLHTMEDIIEWTNEYAPEHLIISTKNYTDI
                     ANRITNAGSIFLGYLTPESAGDYASGTNHTLPTNGYAKAYSGVNLDSFKKKITYQELT
                     KEGIQAIGGTIEVMAENELLTAHKNAVTLRLQSLV"
     gene            13792..14952
                     /locus_tag="PLJ82_02190"
     CDS             13792..14952
                     /locus_tag="PLJ82_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOR40352.1"
                     /translation="MNTPVEKPYTFDRVIRIIITLVVLFALFLLIKKLSTVLLPFLIA
                     LLIAYLLQPVVHFFQYTLKLKYRLLAVISTLILFFGTIVGLLWFITPLASTEIQHISQ
                     TISLYVQGINMDTVLPETWQKEIRIFLSQLNIKSVLENESLMSVLKNSTPKLWLLINN
                     SFSFILGFSVIFIILLYIIFILKDYDKITTQWIQIIPVKYRHITAEIIADIEKGMNHY
                     FRGQFLIAFIVGILFTIGFSIIGLPLAIVFGIFVGILNLVPYLQILAVAPAMLLVFLQ
                     SIETNQPFGSCFLGLLIVFILVQSFQDLVLVPKIMGKVTGLNPAIILLSLSIWGALLG
                     IVGMILALPMTTLLISYYKRFVINKETLTETSEITKEEAKEETIGNNSINIV"
     gene            14958..15272
                     /locus_tag="PLJ82_02195"
     CDS             14958..15272
                     /locus_tag="PLJ82_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOR40353.1"
                     /translation="MILTTTHTIPNREIESILGVARGSTVRTRNIGNDIMASLKNIVG
                     GEIEEYTKLQAQSREQAMQRMIDDAKKMGADAIVGILITTSMVMQGASEILAYGTAVK
                     LK"
     gene            15414..16049
                     /gene="pdxH"
                     /locus_tag="PLJ82_02200"
     CDS             15414..16049
                     /gene="pdxH"
                     /locus_tag="PLJ82_02200"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOR40354.1"
                     /translation="MDNLQDLRKNYEFSYINENQLKDNPLEQFSIWMKEAINSAELEP
                     SAMVLSTVDAQGFPHSRMVLLKDFSESGFVFFSNYESDKAIQMKSNHAVSILFFWQHL
                     ERQVRIEGIVEKIAEIESDAYFLSRPIEHKLGTWASAQSKPIPSSTFLEEQFLYFKDK
                     FKHDIPRPPHWGGYSIKPSRFEFWQGRPNRLHDRFVYKLNESDWTIERLAP"
     gene            16073..17422
                     /locus_tag="PLJ82_02205"
     CDS             16073..17422
                     /locus_tag="PLJ82_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40355.1"
                     /translation="MHKYFVFILSLGLLQNLSAQAASFQAKLDSVIYVKSQKTTYVYD
                     TDDRIEYMLLQGWVEHQNKWDYPTKTSYEYDNTNRLVLSFYSTWNLGSQNYQIQSKRS
                     YSYTEAGTIFQSIASVWSNNQWINSSKQEFSYNANNQILSESEYLWESNKWNTNFKKE
                     YTYDNTGNELSEIFSLPNSQGTGWRLSSKTEKSYTSDNKVISTIRSNYDTSTSKWIPF
                     DKNENSYNTETDLVEKSTKYYWNGDSWEKSAETTYAYNTKNELIRESYKEIESLGSGV
                     SFKTEWTYDDFGNINKEENATWNTSTLGWEYSWKTEFLYNTSYPSSIILSPSDYPTEY
                     KYMISEKRGFDYAEGNWVANTNDALYFYTILHTNIPFVSSITNTVLYINNTTKTLALN
                     HNVSDSATIYIWNMNGELIKSINTKDFPLNINCLSQGVYLYQLVDGKNNFSGKFLKK"
     gene            complement(17427..18353)
                     /locus_tag="PLJ82_02210"
     CDS             complement(17427..18353)
                     /locus_tag="PLJ82_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOR40356.1"
                     /translation="MEHKAIKGHIALFAANILFGLNNPISRSLIPETISPYFLTFCRL
                     VGGMLLFWFFSLFLPKEKVPIKDVILLFFAAIFALSANQLPFIVGLSMTSSIDAAIVI
                     TLLPVMSMVFAFFILREPISSKKVVGVCIGALGAVVLIYSSYYIATGTRNMWGNSIVF
                     SAVIAFSLYLTLFKDLISRYSPVTVMKWMFLFASIQCYPFFHAELMQVSIQSFDMYTF
                     IRIAYVVIIATFITYILLAIGQKVLRPTTLSMYNYLQPIVASLAAVAMGIDSLGIHQL
                     IAALCVFLGVYLVTQSKSRVQIEAQENNHQDK"
     gene            complement(18391..18708)
                     /locus_tag="PLJ82_02215"
     CDS             complement(18391..18708)
                     /locus_tag="PLJ82_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOR40357.1"
                     /translation="MVADILPEAVIIEAINGKNAVERYVEYSPDIILMDVQMPIMGGI
                     EATIEIRKLDMKLGKHTPIIALTANSITEEREKCLNVGMDEFLSKPIATHKLAEFLNY
                     FLS"
     gene            complement(18671..19291)
                     /locus_tag="PLJ82_02220"
     CDS             complement(18671..19291)
                     /locus_tag="PLJ82_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HOR40358.1"
                     /translation="MDIQFFLGLVHNIAVMLLFAWGYDRLWVRFQLHTSVLARVLAGL
                     IMGGICIILMHVPSVVYDGVFFDMRSVFLSTIGLFIGGIPTLLGMIVASVYRLHMGGD
                     GVYMGIASVLSSGTIGLLCRYFSKIPVFQLKWYHFLQLGIVVHVIMCIWVIFLPEDTR
                     MDMFASLSVPILTIYPIANVLLAYLFVTLYKTYSIGCRYITRSCNN"
     gene            19490..>19724
                     /locus_tag="PLJ82_02225"
     CDS             19490..>19724
                     /locus_tag="PLJ82_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF479 domain-containing protein"
                     /protein_id="HOR40359.1"
                     /translation="MNYLAHIFLSGNNQSLQIGNFIADFVKGNQLYSYPADIQEGISL
                     HRKIDTFTDNHPIVLELYTMLNPSFGRYSPILLD"
BASE COUNT         6676 a         3435 c         3174 g         6439 t
ORIGIN      
        1 gcttaatggc aagatcatgt atgtgagaca aatctgatag ataaaaacca ctcataaata
       61 atatcccatt ttttgataaa caagcaacat attgaggcat atattcaagt aaaacattca
      121 ggtttatatt tgcaaaaatc acatcaaatt tctcattatc aactacttct gccccacctt
      181 gaaacacttg tatggtatca actgcattta ccaaaatatt ctcttttgcg tttacataac
      241 accattcgtc aatatcaaca gcccacacct tatttgcacc tctcattttt gctaaaatgg
      301 ctaatacagc tgtaccacaa cccatatcca acactgcctt atccactaat acctcttcta
      361 aaatccatct tagcatcaaa ctagttgttt cgtgatgacc tgtaccaaaa gacatcttag
      421 gatctattac aatatcgtat tttgcattgg gcaaattagt atgaaaagat gatcgtataa
      481 gacatacatc atctactaat ataggcttaa aataatgctt ttcccactct tcattccaat
      541 tttgttgtac aatacgttct tttttaaagc taatttgtgt ttcatataac ttagcaacat
      601 catgaattac ttcactgaaa agagctccct tccaatcagg ctcttgaata aacgcagata
      661 ctccatcttc ttgcaattca aaactctcaa acccaatagc accaagctca tacgcaagaa
      721 tatcagccag atattcggaa aaaggattca ttttgatggc aaatcttaaa taatttatca
      781 tatattagaa tttaataata aaatctttca tttattaatt ttttgttaac aaataaatgt
      841 gtatttttgt atcatagaat cgtacataca cacaacacac attttcacgc atataaaagc
      901 aacaaaagta caaaaaaatc cttaaattga aatgccatga aaaagaaagc tttacttatc
      961 agtagtctgt ttattgcatt ttctatgtcg atgcttgctc aatacgacga catctactat
     1021 aatccaagaa aagcaaatga aacacgaata actacaagta acgaagaaac tgtagttatt
     1081 tctaataatg tgcgtgatgt tgatacttat aaccgtcagg ctccagcaaa ccgaccaaat
     1141 caaacatcga atgtagaaca agatgatttt gagtatacaa aaagaataac acgttttcat
     1201 aacccagaag ccgttactat caacaaccca gaatatgtgt acatatacaa cgacacaccc
     1261 tctgaaccaa atccaactat aaatattcgt gttacactac gctggaatcc atgggattat
     1321 agttatttta cacgttatta ttatccatcc tatcattggt catgggattc aaactatgga
     1381 tggggttata catacaacac ctactatcca ttttatagta catatccttc atcattttat
     1441 tatggttatt atcctccagg ttatggttat tatggttatg ggtactatgg ttacccatct
     1501 tattatcatc atagacccta ctactatcac catccctatc atcataaacc tacttacaca
     1561 acaagaaagg gctataacaa ttacgaccgc acacccaatt atgtaaatga tggcagaggc
     1621 agtagctctt catatggata tcaaaattct ggacaacgtt caacacgttc tgttggaaca
     1681 aacccaacaa atactaacgc atctagtaca cgtaatacat caacttacaa taatagagag
     1741 cgtacattta atagtgcacc atcttcttct acacaaaata ccagaaaaag tgacgtaaca
     1801 acgaatacct ctacaccaag agaaacatct agcggtagaa gtttcaactc ctcttcatca
     1861 tcgtctaata gcaataattc tggtacaaga agtacgcaaa gaagtgataa ttcatcgtct
     1921 tccaacggag gaagcagaag caatggaggt cgctcgttta gcagatagta attatgaaat
     1981 aaataaaaaa taatacgatg aaaaaaataa taattacaag tttgtgtgca ttaggaatta
     2041 tgagctttgc aaatgctcaa tttcagtcgg aatacgatgc actaaaagta gctcaaactg
     2101 acttaaatgg gacagcacgt tttatgtctg tggcaggagc gatgggagct ttaggtggag
     2161 atgcctctac cctattttac aatccagctg ggattgctac ataccgcagt tctgagctca
     2221 cctttactgg aaactggaat tggaacaata cctcagcttt aggcagtatg gctagccgtt
     2281 ttttattcaa tttgaataat gtatcttata tcagttcata taaaacagga tctgagaatg
     2341 gcttagtggc ttttaattgg ggttttgctt ataatagagc aaaaactttt gataaaaaca
     2401 ttcgttattc aactaatagt aatttatcat taggcgactt cttagccaat tatactgaaa
     2461 tttctggaaa tgcagcaggc tatactgcta gtgtttttga tgaagataat gatgtatata
     2521 gagaccaacg tgtgccatgg gcttcggtat tagcatggga tggcatgatg attgcaaacc
     2581 cacaagatga atcgaataat tctatcaata accaatacga atcatataga aataatttaa
     2641 taagtcaaag tattggaggt ggagttcaga atacgtatac catagctgaa cgtggatcag
     2701 taaatgagta tgcatttagt ggaggtgcaa atataagtaa tatactgcaa ctgggagtaa
     2761 gttttgtgtt actcaatctg gattatcaaa tggaatcaat ttttgaagaa aaatttgaaa
     2821 ataatagcac atacaaatat acaagttcat atcaaactga tggttctggc tttagtatta
     2881 aagtaggagc tattgcccga ccaacgcagt ggcttcgtat tggtggtgca ttccactccc
     2941 cttcttggta ttatatcaaa gacaataacg aaggatcaat tggaagtaaa attgtaaccg
     3001 aagtaaagcc tgactacaac ggatcaactc ctgtgggcta tggttcttac tacctaaata
     3061 caccgatgaa aggactagct aaccttggtt ttgtaattgg aaattatgga tttgttggag
     3121 tagattacga gtataccgac tattcaacaa tgaaaatgaa aaataatgat attatcaatt
     3181 cctatatggt ggatcagaat aataaaattg ctaccaattt tgatgctaca cataccattc
     3241 gtgttggtac tgaacttaga ccaattgaaa acgtgtctgt aagacttgga tacgccatgg
     3301 taactccagc aataaaagct gatgcataca gaacatacgc aaccaatacc actcgtacag
     3361 attttgattt tacaaatgat aaaagtacaa attacattac agcaggtttg gggtatcgtt
     3421 ttggaaaatc gtatatggat gtggcttatg tattgcaaac aaaattacaa gattattata
     3481 cctataaccc tacctatgtg aatggatttg gggctcctgt agagattact agcaagcata
     3541 atcaaatttt actaacttat ggagttcggt tttaaataac aaacaatgag aacaaaaaaa
     3601 tggagcactt ttaccaagtt gctccatttt tttaagctgt tatttagata ttacaatata
     3661 gctaataatg tggcattctt cgtaattttc tctacggctt catctatttg atcaaatgta
     3721 tgcgtagcca tcaaggaaat tctgattaag gtatcttcgg atgctactgc tggcgaaaca
     3781 actggattga caaacacacc atcttccata agcatacgag ttaacataaa tgtttttgta
     3841 ttatcacgca catacaaagg aataattggc gtagtagaat gacctgtatc aaatccagct
     3901 ttttgcaatt tagctcgcat ataattggta tttgcccata aagcatccat cctccaagtt
     3961 tctttttcca ttacatctaa ggctgccaat acacaagcgg tagaagcagg tgtaatactt
     4021 gcactgaata tcaatgtacg agaagtatgt tttagatagt taataatatg cttatcagct
     4081 gcaataaaac caccaattgt agctagcgat ttactaaaag tacccattat taagtccaca
     4141 tccttggtta cgccaagatg gttgcagatt cctgccccat gattgccata cactcctaat
     4201 gaatgagcct cgtctaccat gatcgatgca ttgtattttt ttgccaaatc agcaatttct
     4261 agtaattttg cttcatctcc ttccatacta aaaacaccat ccacaacaat caattttact
     4321 ttatcaggct cacatttttt tagttgtttc tccaatgcat ccatatcatt gtgagcatat
     4381 ttaataatct tagaaaatga caaacggctt ccttctataa ttgatgcatg atttgcttta
     4441 tctaaaatca cataatcttc cttgcctgtt aaacaagaaa ctactcccga atttacagta
     4501 aatcctgttg catataccaa tgcttcttct ttacctacta atttagctaa acgagcttct
     4561 aattcaagat gaatatccaa tgttccattt aaaaaacgag aaccagcaca acctgtaccg
     4621 tatttttcta cagctttaat tgcggcttgt ttaatttcag gatgattagt tagccctaaa
     4681 taactattag aaccgaacat taaaactggt ttaccattga tttgtacaac ggtatcttgg
     4741 tcagaagaga taggacgaaa ataaggataa acacctgcct cctgtatttt ttgaggggca
     4801 tcgtattttg ccaacttttg acttaataaa ctcatgcttg cgttaatttt agttacacat
     4861 tttttaaaaa gtgtgcaaat ataccgatta ttttgttatc ttcaattaag tttactaatt
     4921 atttatttag atattgagtg tgagcaatcc agtgggtaaa tccatataga ttgtttttgt
     4981 agattcatct attgaaagga aaaactcttc tgccacagga atatataagg tttcgccttg
     5041 ttccgtatca acacaaaata aggtattggg ggtggaatca tctatagcag taattgcacc
     5101 aattttacca caatttgcat caacaacagt aaagtgtata aagtatgcca catctacttc
     5161 ctcatcatct gtttttctaa gttcattggg caaataaata ggagaatcta ataaaatttt
     5221 tgcgtcttca tccgtattga tatttttcaa tttcaagagt aaggacgtag atgtcttctc
     5281 acgcatatct tctataaaaa aaggcacata aataccctct atattgctca caataaacga
     5341 tagtttacta ctatctactg gaaatgaaaa tgaacacgac aattcacctt taacaccatg
     5401 tgttttatga aattttcctg cttcaaatat ttcttctttt ctaatcatat tttattattt
     5461 tacaaatagt tgttgaacta acgaatgcga ctgaaattca ggagcataca aacattgtat
     5521 tgtgcttatc cctccgctat atacaccatt aaaacgaacc cacaaaggat attcaattac
     5581 ataggtaccc ttggggaatt gactgaaaaa gaaattagta tgggtatctt tggggttctg
     5641 ataataacca attccctctc tgtaacgata tgtagatact tgctctactg gctccaagcc
     5701 actagctcgt aagtctttta tatgtacaaa atccatatcc ctatctgtag atattgttaa
     5761 acgaacaatc actttatctc ctacttgcaa ttgcttattt tcaattggaa caagtattgt
     5821 ttttaatcct cgttgttgtt caacaaacat ttttttatca atattgagtg catgcatgga
     5881 aggttttgta atcttagata cttcctcttc gtattgccag tagatagctc cccacgatac
     5941 attatttcct tttttgttaa cagtaagttt tgataatgaa ggggaaattt cagagttgta
     6001 gaatgtatgc ttacggtatc caattgaaga ctcttctccg ctctcaaaaa gtacattatt
     6061 attgactttt acaatcatac tagacggttg aacaatactc attgagtttc cagtaaacac
     6121 taaagcataa atagcatcta ctgtagctat tggattattc catgcattgg tttgtttatt
     6181 ggtaagtaac catattttaa gtttgtcttc tatttctttg ttaggtttaa tttcggcaat
     6241 tgcttccatt atagcaacat gcgttacaat gggcatttga taccaagtat agccggcagt
     6301 attatttgcc cagaatattc ctttatcctt gttttcagtc gcaaattcga gtaatgaagt
     6361 aacaatactt tgtgctaatt ttttttcatt atttctaaat aagattctgg caacttgcga
     6421 ttttccatac aaagaatagg ctgtccaatt ttgctttaaa actgcattaa agtatattat
     6481 tgatttattc agctcttctg actttggaat agttgaaaaa agcgacctca catacaatgc
     6541 ttgtagtgta tatgcatcaa caacgaatgg tttgtttgta gaatgtttta tgtaagtatt
     6601 gtatgacttg attatctgat aatcaatata cgctactgct cgctgcatca tctctgtacc
     6661 aaaaattgtg gagtctaaag cttgtatcga cactaatttt gctattttct ctgctataaa
     6721 caacgttacc atttcattct caggcatacc agcaaaccaa gaaaaacccc ctgttggcat
     6781 ctgtaattct tgcaattttt tccacaattc ttgttgttta tgatgcaatt gatttgtgtc
     6841 caaatatagt tgcaacttag ataacccttc cgtttcttgc agggcatctt gtatccacgg
     6901 actttctgag ttgaacaaat ttttcagttc ttcattttta actaagggcg actcttcctt
     6961 attggcataa agctgaatat attgcttaaa taatggtgta tttgctacga caaactcacc
     7021 aagcatatta gcataataac ttgacataag agaaatagca ttatcgtatt ctggctcagc
     7081 tactacaggt aatgcctgta aggcatacca tgctacattg ggagataatt caatcgtata
     7141 acgtttgtgt gataatgttg tagatgtatt gttattcagt ttatcaaatt caaatgtata
     7201 ttccccttcc ttgtacatag taaaaggcat actctctgta actaattttt tgctcgacaa
     7261 gatgggtaaa taatgttgtt ccccatcgct atacgagtca tttgctacca tcacttttac
     7321 tacccaaaga gaggaattag ttggcacgaa tagtggtaat ttaattacaa ccgattgatg
     7381 tggattcaga gttactttct cttcattgga cagtaatata ctgtccgttt gtgcatctct
     7441 tatttcccat ttagcagtag ttttagttag tgaatccaac atattgtaga tagtagcggt
     7501 taacacaatg ctatcacttt gttgaacaaa acgaggtaaa tttggtttca cagaaatagc
     7561 tttttgggta tacacatacg atgtttgttg tccataatac gcatctgcat tatgaacgag
     7621 tgtcattatt ttccatgttg ttaaagactc tggaaccgta aaacgcaccg ttgccaatcc
     7681 ttcattatct gtttttatgg ctggataaaa gaacgctgtt tctgcaaatt ctttacgaat
     7741 aggcattttt tgagataatt gcgaagatgc agaagcagtt gtttcagtag aaagaacttt
     7801 gtcttctact acttcatcca ttacatcatc tattacctct aatgatttcc gcaacatatt
     7861 gcctccaaca gccatatacg gcatagcata acgacaatac ccaggatatt gaaagtcaaa
     7921 cagattaatt ttgggtaatt caaaaccttt ttcatccttg aaaggaacaa tacctgtagc
     7981 ataatttgaa cgagaaaaag aaggtgttgc attccaagat ggaacatacc actgatgtga
     8041 aacaaacgga ttaaattgcc actgatgcgg tacaatagca tctaacgaag catcgtacat
     8101 acacacaagc acctctgctt gctgattaac tccatttgca tcttttacat gtatttgcca
     8161 ctcttcttgg tttcctggtt gtaatttatc tcgaaaagta atatactgaa tagatatttt
     8221 ttttgaaggt tgccgtttta ctattcggtt gctttgctga tacaaagtat tatttacaac
     8281 agatgtaaaa cgcatttcta cactattgcc acaattttta gtatacggaa attgaatatg
     8341 tttactttcg ttgtttaaag taataaactg acggcagatg agtccgtctt ctccaactaa
     8401 ttcacacata accaacacat tagctgaaga accaatgatg gttgaagcca tttctcccac
     8461 agagcaggta tctgataaag catacatcca gtttgatgtt ttgaatggag gttttttatc
     8521 ttttaccgaa aataatgtaa agaaatgtat gttttctact tctgttaggc tctgcgtttt
     8581 cgttgagctt attagtttgt atctacctga aggtaaattg gatactatat ttaacgaaaa
     8641 tggttttccc gacacaaaag agccttcata tacacactta ctcagttgta gactatcaac
     8701 aggagtatca ttatttgagc tttcttttaa agcataaatt gcaattttgc cactttgatg
     8761 aagcatttct ccatttacat tagtaacatt taccgctatt ggagttgttt tttctttttg
     8821 aataattgca ggaatattcg tttgtatata tatggcactt tctccgattg aaaaactcga
     8881 agacacttgt tgtgtttcac cagctttgtc ggtaatatca acatatacaa gataatgata
     8941 ataagcactc tggtcggtat caggttttat cggaataaaa tcgaatgtaa actcgccctt
     9001 atcatttgtt ttgaaaacac cttctgccac gttctctgtt gtgtggatag ggaaatacct
     9061 acgccaagaa aattgttgtc gaacaatgcg gtatttacca tgtgcagcct gtaaaggaaa
     9121 gcccatgtat gtttcagcat ttccatttat gcgaacagga ttattaaaca atacttcttt
     9181 atctattcgc ttaatttcag ctttaaaagt aggtttttta tattcttcta ctaaaaatga
     9241 gctagatcca ttttctgttt cgagtgtaaa actccctgtt agccctgatt ttggtaatat
     9301 aaaatcagta ttaaaagagc cgtaacttga tgtttgcact tgtttttctg caataatttg
     9361 gtagtttgca tcacgcagtc taatgtgtat ttttttgttt ttttctaaaa ttacagagtc
     9421 ttcttttaca ttccatgcaa tcccttttac atacacactt tgtgctggac gatacaatgc
     9481 cctatctgta aacagtgcaa tatgttgttg catatctggc ggggtattct ctttttgttt
     9541 gccgtacaca tacacattac cgataggcgt gctagtatcc tctccttgcg atacgcttag
     9601 gtaataagca ttgttatgct gtaacgaaaa ggtagctaat ccgtttttat tcgtattggt
     9661 tgatgctact ttttttacgt caacattgtt ccgttgatag cgaacttgat gaacagtaac
     9721 agttgcgttt ttgataggtt taccagttat tctatctact acccacccct ctattgtctt
     9781 attgatagac ttatgcacca gcaacaaacg cgttacataa aaagtatttt ttatgctatc
     9841 aaaagcattg gttggagata ctatttcata ttcatacaaa cccaagtctt tggttttaat
     9901 gtgaatactt gtatctttgg atagaaacaa ttctgatact ggtaaggtta catgtatagt
     9961 attcactagt tctctatcaa tggcttgacg ggttatgcca taataatttt ttacaatttg
    10021 atactccaac gaattactac gtattttata taatcgaatg gttaatgagt caatatttga
    10081 aaaatttagt ttcaaattca acgatgaatc tggatacaca acagattggc ttgatgttgc
    10141 taattgtggt tgtttaattt gttctactat attgtgtaat actgcaatgc gagagtaatc
    10201 gggaaaacgg ttgatgtata acttacacaa atcgtatgcc tcttttcttt tgttttcact
    10261 caacaaatag tttgctttta aaacaatagc atgtaccaca ataggagatt tttgaaactt
    10321 ttcaatgata ctatttaatg taagttggta agattcagat actttttcag aaggaataga
    10381 taacgcaatt aaggtttcta ttctatcaat atctgttttt accaaagcat cagtattacc
    10441 attcgcttta tgaaatgtaa gaagtttttg gtacacattt tttactcgtg gtactacttg
    10501 ccatagcgaa gtagttgtat ctgcctcagc aataaaatga tcgtaaggag ttataagaga
    10561 catttcagtt gatttctctt gtgttaccaa acgttcaatc tgatgtttat tctgtacata
    10621 taattgtatg taattatgtg ccaacacatc atataccgta ggtcgtaaat aactatcagt
    10681 agcttgcact aaaatggtag gataattggt aacaagtgtt ttttgtaagg ctacttcatg
    10741 ttgaatagaa agtgaagcat aatggaatat cctgtataaa aaatgattac tagcccactc
    10801 ttttttattt tctggtacta tatcccatat aggcgttcgc tgctgaatga tatacgagtt
    10861 tgcgttgaaa tacgaatcat atacttgtgc caacatggca tacagtactg attgtgaaat
    10921 aacatcctta gaggaactgg catatctctc tatttctgca acttgatcaa aaaaactatc
    10981 atgatcaatc tctacattcg tttttaaatc atatatcaaa caacgtataa tttcttcgtg
    11041 ggcatgctca gatagtgctt tttgtttgat tttttgtact atctcgttgg cagatttagg
    11101 cataccatgt gtttgcaaag agtccacttg ttgccaataa gtaggatacg acatagcaac
    11161 agattgttgt ttggctaaca gaaatgacac aaatgtaagt gaaaggagta atgttatgaa
    11221 tatccctatt tttttcatac gcaatagtat atattttttg atagaatgac tacaaagtaa
    11281 caaattatta gcgatactca gcataaaata aaacataaaa aaacttagct attgaatatt
    11341 aacctaaaat ttgggtatat aaaaaattta cgttatcttc gcaccataat tatgagaaat
    11401 agctataccc atattacaca tcattttcac ttacactaat agtggtaagc cgaagggtgt
    11461 ttgggttata gaaaacttat aacagtaggc ttgccacttt ggtaagcttt ttttattact
    11521 aaaccaaata agaaatatat gttacgaatt gcagtacaag ccaaaggtcg tttatacgaa
    11581 gaaacgatgt tgatgttatc agaatcaagt attaaaattg aatcaggaaa aagaacgtta
    11641 ttaatccccg caaaaaactt ccctatagaa gtattatttt tacgtgatga tgatattcca
    11701 caagcagttg ccagcggtat agcagatgtt ggagtagtag gagaaaacga gtttgccgaa
    11761 aaacaagaag atgctgaaat tgtaaaacgt ttggatttta gcaaatgtcg tttatcatta
    11821 gctataccta agtcaataga ttatcaagga gttcattggt ttgaaggcaa gaaaattgcc
    11881 acttcgtatc ctgttatttt agagaatttt cttaaaaaac acaacataca agccgaaata
    11941 catgtaatta ctggttcagt agaaattgct cctggcattg gcttggctga tggcattttt
    12001 gatattgtaa gttctggtag cacattggtg agcaataact taaaagaagt ggaagtggtt
    12061 atgaaatctg aagctattct tattgccaac aaacaactta acaacgaaaa aagagcgatt
    12121 ctagaagaat tattattccg ttttaatgcg tataaaaatg ctgaaggaaa aaaatacatt
    12181 attctaaata tttctaatga caaaatagat gccgtatgca agcttttgcc tggtatgaaa
    12241 tcacctactg ttacgccatt ggcagaagaa ggatggtcat cactacattc ggtaattgag
    12301 gaaaaaacat tttgggacat aatcaaccaa ctgaaagcat taggagcaga aggaatattg
    12361 gttgttccaa ttgaaaaaat gattctgtaa taatttacaa aatttttgtt tatgtactat
    12421 attcaatatc cagaaaaaaa ggagtgggca agtttacttg cacgtccaac ctttgacaca
    12481 acaaatttat ttgataccgt taaccatatt ttagtcgacg tacaaaaacg tggagataag
    12541 gctgttatcg agtacgaaga aaaatttgac aaaattcatt tggatgtatt gcaagtaagc
    12601 gataacgagc tagcaagtgc agaaacaaaa atcagcaaag aattaaaaga tgctatttta
    12661 atagcaaaaa aaaatattgc aaattttcat gcagcccaag tgtatagcga tgttacaatt
    12721 gaaactatgc ctggtataca ttgttggcaa aaaaatctac caatcgaaaa agttggatta
    12781 tatattcctg gaggtactgc tcccctattt tctacggtac tgatgttagc aattccagct
    12841 caaattgctg gttgcaaaga aattattctt tgttcaccac caaatagcaa tggagatata
    12901 cacccagcaa ttttatttgc agcacacatt tcgggagtaa gtaaggtata taaaattggt
    12961 ggagtgcaag ctattgcagc aatggcatat ggcactgaaa ccgtaccaaa agtatacaaa
    13021 atttttggtc caggaaatca atatgtaaca gctgctaagc aacttgtaag tttaaaagat
    13081 gtggctattg atatgccggc tggtccatcg gaagtagaag taattgctga cgaaacagct
    13141 aaccctgcgt ttgttgctgc agatttgctt tcgcaagctg agcatggggc agacagttct
    13201 gctattttgc taacaactag caatgaatta gcaactgctg tgcaacaaga aatacagcaa
    13261 cagcttcctc ttttgtcgcg tgctgaaatt gccgttaaag cactgaataa tagcaaaata
    13321 attttgctac atacaatgga ggatatcatt gaatggacaa acgagtacgc tcctgaacat
    13381 ttaattatct ctacaaaaaa ttacacagat atagccaata gaatcaccaa tgcaggttca
    13441 atatttttgg gttacttaac acctgaaagt gctggcgatt atgcttcagg cacaaaccac
    13501 accttaccta ctaatggata cgcaaaggca tatagtggtg taaatctaga tagttttaag
    13561 aaaaaaatta cgtatcaaga attaacaaaa gaaggcatac aagccatagg aggcactatt
    13621 gaagtaatgg ctgaaaatga attgttaaca gcacataaaa atgctgttac gctacgctta
    13681 caatcacttg tataaatata tagttaattt ttgctatttt tattaaactt ttattccatt
    13741 ttatttgtat gtttgtaatt gaaatataac gatagtaaaa acattctgaa aatgaataca
    13801 cctgtggaaa aaccatacac attcgaccgc gttatccgca ttattataac gttggtagtt
    13861 ttgtttgcct tatttctact aataaaaaaa ctaagtacag tactgcttcc ctttttaatt
    13921 gccttactta ttgcatattt actacaacct gttgttcatt tttttcaata cactctcaaa
    13981 ttgaaataca gacttttggc tgtcatcagt acactgatac ttttttttgg cactattgta
    14041 ggtcttttat ggtttatcac tccattggca agtactgaaa ttcaacacat ttctcaaact
    14101 atatcattat acgttcaagg tataaatatg gatacggtat tgcctgaaac gtggcaaaaa
    14161 gaaattcgca tatttttaag tcagcttaat ataaaatctg tattagaaaa cgagagtttg
    14221 atgtctgtac ttaaaaattc tactccaaaa ctatggctat tgattaataa ctcttttagt
    14281 tttatactag gattttcagt aatatttatc attttattgt acattatttt tatcttaaaa
    14341 gattatgata aaattacaac acagtggatt caaatcatac ctgtaaaata tcgtcatatt
    14401 actgctgaaa taattgcaga cattgaaaaa ggaatgaatc attattttcg aggtcaattt
    14461 ttaatagcct tcatagttgg cattcttttc acaataggtt ttagcattat tggtttgcct
    14521 ttggctattg tttttggtat atttgtagga attttaaatt tagtacccta tctgcaaata
    14581 cttgccgtag cacctgctat gttactagtt tttttacaat caattgaaac gaatcaacct
    14641 tttggttcct gttttttagg gttactgatt gtttttattc tagtgcaaag ttttcaagat
    14701 cttgtattgg ttcctaaaat aatgggcaaa gtaacaggtt taaaccctgc cattatttta
    14761 ctttctcttt ctatttgggg tgcattgctt ggcattgtag gtatgatttt agcattacct
    14821 atgacaacgt tattaatttc ctattataaa cgatttgtca tcaacaaaga aacattaaca
    14881 gaaacatctg aaataacaaa agaagaagcg aaagaagaaa ctatcggaaa taactcaatt
    14941 aatatagttt gaaagctatg attttaacaa caacacacac aattccaaac cgtgaaatag
    15001 agagtatttt aggtgtggct agaggtagta ctgttcgtac acgaaacatt ggtaacgata
    15061 ttatggcaag tttaaaaaat atagttggtg gtgaaattga agagtataca aaacttcaag
    15121 cacaatcacg agaacaagct atgcaacgca tgatagatga tgcaaaaaaa atgggagccg
    15181 atgcaattgt tggtattctt attaccacat ccatggtaat gcagggagca tcagagatac
    15241 tagcttatgg aacggctgta aaattaaaat aatatgatgt atgtagtatt agggttacag
    15301 gcattatcag ctgtaattat attcgttatt atagttttct taattattcg tagaagaaaa
    15361 attaagaaaa cagaaacctt tgaagataga agtaactaaa aaaacactca tatatggata
    15421 acctacagga cttacgcaaa aattatgaat tttcgtatat aaatgaaaat caattaaaag
    15481 ataatccttt ggaacagttt tcaatttgga tgaaagaagc tattaatagt gctgaattag
    15541 aaccttctgc catggtttta tctactgttg atgcacaagg ttttcctcat tcacgtatgg
    15601 tgcttttgaa agacttttca gagtcaggat ttgtgttttt ttctaattat gaaagcgaca
    15661 aagctattca aatgaaaagc aatcatgctg tttctatttt atttttctgg caacaccttg
    15721 aacgtcaagt acgtatagaa gggattgtgg aaaaaattgc agaaattgaa tcagatgctt
    15781 atttcttatc tcgtccaata gagcataaat tgggtacatg ggcatcagca caaagtaaac
    15841 cgattccttc ttctacattt ttagaagaac aatttttata ttttaaagat aaatttaaac
    15901 acgatattcc tcgaccacct cattggggtg gatatagtat taagccatct cgttttgaat
    15961 tttggcaagg cagacctaat cgtttacatg atcgttttgt atacaagtta aatgaatctg
    16021 attggactat tgaacgttta gctccttaat tatcttttaa aaaaattgtt ttatgcataa
    16081 atacttcgtt tttatactta gtcttggctt attgcaaaac ttatctgcac aagcagcgtc
    16141 ttttcaagca aaattggata gtgtgattta tgttaaatca caaaaaacca cgtatgtata
    16201 tgacactgat gacagaatag aatacatgct actacaagga tgggtggaac atcaaaacaa
    16261 atgggattat ccaactaaaa caagctatga atacgacaat acaaatagat tagtactctc
    16321 attttattcg acatggaatc ttggttcgca gaactatcaa attcaatcga aaagaagtta
    16381 tagctatact gaagctggta ctatttttca atctatagct tctgtttggt ctaacaatca
    16441 atggataaat agctcaaaac aagaattttc ctacaatgca aacaatcaaa ttttatcgga
    16501 atcagaatac ctttgggaat cgaacaagtg gaacactaat ttcaaaaaag aatatactta
    16561 cgacaatact ggaaatgaat tatcagaaat tttttccctt cctaacagcc aaggcactgg
    16621 ttggagatta tctagtaaaa cagaaaaatc gtatacctcg gataataaag tgatatcaac
    16681 tatccgctct aattacgata ccagcacttc aaaatggata ccttttgaca aaaatgaaaa
    16741 tagctacaat acggaaacag atttagtaga aaaatcaact aaatattatt ggaatggaga
    16801 tagttgggag aaatcggctg aaacaacgta tgcgtacaac accaaaaacg agttaatacg
    16861 cgaatcatac aaagaaattg aaagcctagg aagtggtgtg agttttaaaa cagaatggac
    16921 gtatgatgat tttggcaata tcaataagga agaaaatgct acttggaata ctagtacttt
    16981 aggatgggag tatagctgga aaactgaatt tctctacaat acatcatacc catcaagtat
    17041 tattttgtct ccttcagatt accctactga atataaatat atgatttctg aaaaaagagg
    17101 ttttgattat gcagagggaa attgggtagc aaatacaaat gatgctcttt atttttatac
    17161 catactccat acaaacattc cttttgttag ttcaattact aacaccgttt tgtacataaa
    17221 caatactaca aaaacattag cacttaacca caacgtatct gatagtgcta ctatttacat
    17281 atggaatatg aatggagaac taattaaatc tatcaataca aaagattttc ctttgaatat
    17341 caattgttta tcacaaggtg tttacctata ccaattagta gatggaaaaa ataatttttc
    17401 tggcaaattt ttgaagaaat aatcagttat ttgtcttggt gattgttctc ctgtgcctca
    17461 atttgcaccc ttgatttact ttgtgtaact agatatactc ctagaaacac acacaaagca
    17521 gcgattaatt gatgtattcc taaactatca atacccatag caactgccgc caacgatgcc
    17581 actatgggtt gtaggtagtt gtacatactt aaagtagtag gacgcaatac tttttgacct
    17641 atagccaaca aaatataggt aatgaaagta gcaataatta ctacataagc aatgcgaata
    17701 aaagtataca tatcaaaact ttggatagac acttgcataa gctctgcatg aaaaaaagga
    17761 taacattgaa tgcttgcaaa taaaaacatc catttcatta ctgtaactgg agaataacga
    17821 gaaatcaggt ctttaaacag cgttaagtat aatgaaaatg caataacagc actaaataca
    17881 atactattac cccacatatt gcgcgtacca gtagcaatgt aataagaact ataaattaac
    17941 actactgctc ctagtgcacc aatacaaaca cctactactt tttttgagct tatgggttcc
    18001 cttagaataa aaaaggcaaa caccatactc attactggta gcaaggttat tactatggct
    18061 gcatcaatag acgatgtcat tgataaaccc actataaaag gcaattgatt ggctgataac
    18121 gcaaatatgg cggcaaaaaa gagtaaaata acatctttaa ttggcacttt ttctttaggt
    18181 aaaaacaaag aaaagaacca aaacaaaagc atccctccaa ctaatcggca aaaagtaaga
    18241 aaatacggac ttattgtttc tggaattaac gaacgagaaa ttgggttatt taatccaaat
    18301 aaaatatttg ctgcaaataa tgcgatgtgc cccttaatgg ctttatgttc cataattttc
    18361 ctaaatgata ataactcaaa ccaatgtatg tcaacttaaa aaataattta gaaattctgc
    18421 taatttgtgt gtagcaattg gtttagataa aaattcgtcc ataccaacgt tcaaacattt
    18481 ttcgcgttct tctgttattg aattagctgt aagagctatg ataggcgtat gtttccccaa
    18541 cttcatatct aactttctaa tctctattgt ggcttcaatt cctcccatta taggcatttg
    18601 aacatccatt aaaataatat ctggtgaata ttctacatat ctttccactg catttttacc
    18661 attaatagcc tcaattatta cagcttctgg taatatatct gcaaccaatg ctataggttt
    18721 tatataaggt tacaaataaa tatgccagta aaacattagc aataggatag atagttaaaa
    18781 ttggtacaga caatgacgca aacatatcca ttcttgtatc ctctggcaaa aaaataaccc
    18841 atatacacat aattacatgt acaacaatcc ctaattgcaa aaaatgatac catttcaatt
    18901 gaaaaacagg aattttagaa aaatagcgac acaataaacc gattgttcca gaagacaaaa
    18961 cagaagcaat tcccatatat accccatctc cacccatatg taaacgatat acagaagcta
    19021 caatcattcc taataaagtt ggaatgcctc ctataaacaa cccaattgta ctaagaaata
    19081 cagatcgcat gtcaaaaaaa acaccatcat acacaacaga cggaacatgc attaatataa
    19141 tacaaattcc tcccattatt aaaccagcca aaactcttgc caatacactc gtatgtaact
    19201 gaaatctaac ccataatcta tcataacccc acgcaaataa caacatgacg gcaatattat
    19261 gaaccagacc taaaaaaaat tggatatcca tatcagcaac gaattgtttg taaattatct
    19321 aataaacact acaaatgata ctctatttac aaaagtaaac atatatttat aaattaagtt
    19381 attaattcat caaaaaataa cgataatcct aacagtagat gttatgttag gattatttgc
    19441 gtaattttgt tgttcataca catactatat actatcaaaa ataaaagaca tgaactattt
    19501 agcccatatt tttttgtctg gaaacaacca atctcttcaa ataggcaatt ttattgctga
    19561 ttttgtgaaa ggaaaccaac tttattccta tcctgcggat attcaagaag gtattagctt
    19621 acatcgtaaa atagatactt ttacagacaa tcacccaata gtactggaat tatatactat
    19681 gttaaaccca agttttggtc gttattcacc cattctgttg gata
//
LOCUS       DAOSRP010000019        19050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3046_length_19050_cov_4.910608, whole genome shotgun sequence.
ACCESSION   DAOSRP010000019 DAOSRP010000000
VERSION     DAOSRP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3046_length_19050_cov_4.910608"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..646
                     /locus_tag="PLJ82_02230"
     CDS             230..646
                     /locus_tag="PLJ82_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40360.1"
                     /translation="MNKAQAQTHVIRFKRYSRKAYAAFCSMHKHITIGKLATALCDKQ
                     LAKSKLAHTNSADCLAFYTPVFSDESIVDNEMFAFDVLPLGCVAVIPTTYSTTEGQEA
                     RISCSDCLYTLLLYLMVFSTAFLFAHTLYSLNPFMI"
     gene            643..1698
                     /locus_tag="PLJ82_02235"
     CDS             643..1698
                     /locus_tag="PLJ82_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOR40361.1"
                     /translation="MNKKKILSLALLATLLPQLGMMAQSNSSNTQQLTEISVHAQPTK
                     VYSDHLRVVMSIDKSTIAQMPVQTIQELLNNLPGLDVRQRGGSGVQADISMRGGTFDQ
                     VLILLNGVAISDPQTGHHTLNLPIDLNMVERVEILQGGAISLFGTNALSGAINIVTNE
                     KADDALKVSLTGGSFGLFNPSVSSTFASNKWRWAISASHNESAGYTENTDYRYTQGFV
                     QAQFQATEQDIWKWQIGVQDKAFGANSFYSVNYPNQFEKTKTLISSISWDKRINSLLW
                     QSTAYYRGHFDQFELFRNYENAATWYTFHNYHLSHVSGINSKLSWFTSLGKSSIGIEG
                     RNEHINSNVLGELKATP"
     gene            1702..2565
                     /locus_tag="PLJ82_02240"
     CDS             1702..2565
                     /locus_tag="PLJ82_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40362.1"
                     /translation="MPFAKNTNTQFLYEKNRTNVQYFAEQTIYLQAFTASVGVSGNYN
                     TQFGNRYSYGLNTSYAFNTYAQVFANINKSLRLPTFTDLYYKSATQESNPSLKPEEAI
                     TAEIGAKYTKQAWQWNANAFYRIANNSIDWVRSLETEKWKSMNHTRIDAMGGEIAAAY
                     TGGFWAKNIQASYAYVLLNKDAGELISKYALDYLQHKINLQYVHGISKQVDASWVVSY
                     QDRKGNYVNKNGNKVAYTPFATIDTRINWQQNNTKMYAEISNILNQHYYDYGGVPIAG
                     RMLKVGIAVQL"
     gene            2731..3018
                     /locus_tag="PLJ82_02245"
     CDS             2731..3018
                     /locus_tag="PLJ82_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR40363.1"
                     /translation="MNKPTIFHFFRPLITLFILLHVALVSILAQTVDLKTTTFNAEWL
                     SCDTNGPSDEEGQMNNIVSLIQTINPDIIALQEVGTSTTYATIDTLVKHLE"
     gene            3039..4391
                     /locus_tag="PLJ82_02250"
     CDS             3039..4391
                     /locus_tag="PLJ82_02250"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOR40364.1"
                     /translation="MATTHTNCGQNQGIIYKKFHLQLVNSSLLSNGANSQGNSYYYNW
                     TNGRYPVIHDFVLTIGSTNIPFSIINIHAKAMSDETSYSRRLGASEGLKGILDGTNYN
                     TSKIIITGDFNDYLIGSQTSSYSNSPYKNFMDDTENYQSLTSSLTDPYYSSPVIDNII
                     ITNELASHHVANSTIRETSATNTISNYRNTTSDHTPISALFRFTSNNPTDCQAIDYSE
                     SFASSLGDFIPYSVEGTQTWHWRNIYGAYVSGYASSTNNPNEDWLISPAFDLSDKESG
                     SLSFEHALNYAQEANERASNHTLWISTNYTEGDPKNANWTQLSIPTMPLGTSWTYVNS
                     GSINLPSQWMKENVRFAFKYMATSSMAGTWEIKNLLFNATCEPVAIPSISNDNKVVFA
                     QNRNITVLLEHETSIRIFNILGNTLYNDTRSENSVFPVSQAGIYLVCIGNECYKIAVR
                     "
     gene            4497..6071
                     /locus_tag="PLJ82_02255"
     CDS             4497..6071
                     /locus_tag="PLJ82_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR40365.1"
                     /translation="MKTPILSILLLVGWFATFAQTSAQFEMQKLNFSYGAITNFYVDK
                     VDPSKLTEWAIKGMLDELDPHSSYLSADEVKESTEALQGSFDGIGVSFNMVEDTLVVI
                     QSITGGPSEKLGILPGDKIILVNDTTIAGVKMKTSDITKRLKGPKGTQVKVTILRNNE
                     SIPFTIIRDRIPIFSVDAFYMVDNKIGYIKVNRFASTTHQEVVDGIKNLKKKGMESLI
                     IDLQGNGGGYLQSAIEIVDELLPNKRTVVYTEGANHAKQTMTTRRNGLMESGKVVVLV
                     DEYSASASEIVAGALQDWDRGVVIGRRTFGKGLVQRPIPMPDGSEIRLTVARYYTPSG
                     RSIQRPYEKGKEKYEEDILDRYKHGEFVSADSITFADSLTYKTLVTQRTVYGGGGIMP
                     DIFVPADTTKYTTLHRQLIRNHIITKISIVYAEKNRALLSKQFSTFEAFKLKFDTQEV
                     LSHIKQETLAKEIAFTEEEWNETKDLLAIQAKAIIARNLWSTNEYFEIINETNDFVKK
                     AIEVLKSESIYYNTLQ"
     gene            6090..6974
                     /locus_tag="PLJ82_02260"
     CDS             6090..6974
                     /locus_tag="PLJ82_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOR40366.1"
                     /translation="MKNKNYASDIKTVIDELTGLCPTENMFHPNKEGEPFPSRAIMKD
                     SIKLTRSILFPGFFGNASVNKTTLPYHIGVNVEKLFTMLWEQVYAGLCFDCKEEDIDL
                     EAKREKAFTITLSFVKQLPRIKEVLRTDVEAMYKMDPAAKSYGEIIISYPGLRAITNY
                     RIAHELHKLQTPIIPRIITEMAHAETGIDIHPGARIGESFAIDHGTGVVIGETCIIGN
                     NVRIYQGVTLGAKSFPVDENNIPIKGIDRHPIIKDNVIIYASATILGRIIIGENAVIG
                     GNTWITEDVKPNEKVFVK"
     gene            complement(7064..7534)
                     /locus_tag="PLJ82_02265"
     CDS             complement(7064..7534)
                     /locus_tag="PLJ82_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOR40367.1"
                     /translation="MESTSFMQLAIDLAIENVKQGKGGPFGAVVVKEGKIVGQAANSV
                     TSTNDPTAHAEVNAIRQACAHLKTFDLSGCELYASCEPCPMCLAAIYWARIDKVYVSA
                     TKHDAAEVGFDDAFIYEEFALPKENRKVLQVPLLVQEGKAPFKAWKQKTDKIPY"
     gene            7692..9830
                     /locus_tag="PLJ82_02270"
     CDS             7692..9830
                     /locus_tag="PLJ82_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR40368.1"
                     /translation="METNTELELAWRCVVQTGTHLFLTGKAGTGKTTFLHQLKTTSPK
                     RMVVLAPTGIAAINAGGVTIHSFFQLPFAPYIPGTEFATDTKYQFNKEKINIIRSLDL
                     LVIDEVSMVRADLLDSVDAILRRYRRTTVPFGGVQLLLIGDLHQLSPVIKEEERRLLE
                     AYYPSSYFFNSKALQKTAYLTIELKQVYRQSEKHFLDLLNQIRDNKATSTTLEELNKR
                     YIPDFIPPAGEEYIRLTTHNYQAQTINDQHLAKISSPAFSYKAQVNGNFPQHAYPADE
                     LLTIKEGAQIMFIKNDASFPKRFYNGKIGKVTHIDEHVIEVQTNDDPEPFSLQKAVWT
                     NAKYVLNKETNEISEDIEGTFEQYPIRLAWAITIHKSQGLTFDKAIIDIASSFAHGQT
                     YVALSRCRTLNGMVLNSPISHRAIIKDAVIHDFVQQSATDAPDESKIAVLERAYFSYL
                     VTEMVQFSDITKLLVRGSYLFKESPLIGMYPSIHEQLKQSIEHIQQDIEQVSSRFLSV
                     FFSMFRNTPDYQDNAALQERIQSAASYFLVQLNTLVAPLIAAIKVDVDNKETEKQVND
                     LHAQLSETLQIHTACLSSCKNNGFSVSAYQKAKLEAAIEHTSTKKRTAKASEKQKVSI
                     NSGETEHAQLYEHLRAWRLQEASERKLPAYAILHQKALLGIVQHLPLSPTELLQIPHL
                     GKRTVTKFGDAIIEIVREFVQSKENDKTNS"
     gene            9981..11060
                     /locus_tag="PLJ82_02275"
     CDS             9981..11060
                     /locus_tag="PLJ82_02275"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOR40369.1"
                     /translation="MKNLHIGDLVIPVPIIQGGMGVGISLSGLASAVANQGGVGVMSA
                     AGLGLLYRHLSSDYPEACILGLREEIKKARDKTKGIIGVNIMVAMTNFADMVKTSIAE
                     KVDIIFSGAGLPLDLPSFLQKDSTTKLVPIVSSARAAKLICDKWKAAYNYLPDAVVVE
                     GPKAGGHLGYKESQLSDDAYRLETIIPEVVATIKEIETTHDCKISVIAAGGIYTGADM
                     YDIMQLGADGVQLGTRFVTTTECDASEIFKQAYIDAAEADVEIIKSPVGMPGRAIFSD
                     FLQKVKDGEKQPKSCPFHCIKTCQIETSPYCIMLALFNAYKGNMTMGYAFAGTNAHLA
                     TAIVTVKDTMDAIQEEYKAREKELV"
     gene            11167..11577
                     /locus_tag="PLJ82_02280"
     CDS             11167..11577
                     /locus_tag="PLJ82_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOR40370.1"
                     /translation="MEELTLTTPSLLFSAVSLLLLAYTNRFLSYAQVVRTLKEKYTKD
                     HSAITGAQINNLRKRLYLTRAMQVQGIVSLFLCVVTMLLIYIQLQLIAAYVFGVALFL
                     LILSLGTSIWEIYISVKALEIYLSDITNELDNKK"
     gene            11707..12651
                     /locus_tag="PLJ82_02285"
     CDS             11707..12651
                     /locus_tag="PLJ82_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOR40371.1"
                     /translation="MNLLKNNIATFFKGFAMGTANVIPGVSGGTIALILGIFERIIDS
                     LKCFDGEAIKLLIKGKFKAFATHTDLGFLSVVLLGVIVSIFTVAKLLSFLFLNYPIFI
                     WSYFFGLIIASIWLVGKTISSYNTSVILFLILGTAIAASITILNPASENDSFFYLVIC
                     GIVAVCSMILPGISGSFVLVLMGNYELVLRSINEFNITNLVAVAIGCAIGLPAFSHLL
                     SWLYKTYKNQTIAMLTGFILGSLAVIWPWKYSYDTVGNLVEANKFGALVNTDVKIISY
                     ERILPHIDGLFFIAIIIAILGFLSIYLLEKKAIHMKEE"
     gene            12745..14013
                     /locus_tag="PLJ82_02290"
     CDS             12745..14013
                     /locus_tag="PLJ82_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOR40372.1"
                     /translation="MASLSVHDIEIMAPAGSWESLYAALHAGADSVYFGIENLNMRSH
                     SSNNFTINDLREIVRLCHEHKVKSYLTINTILYDEDIELMHQIVDTAQEEGISAIIAA
                     DVAVLTYARSKGVEVHLSTQLNISNVDALIFYAQFADVVVLARELTMNQVAHIFKTIN
                     DRNICGPSGNRIRIEMFCHGALCMAVSGKCYLSLHEMNKSANRGSCMQICRRSYEVKD
                     KELGIELEVDNEYIMSPKDLKTIHFVNKMIDAGVRVFKIEGRARSAEYVKATVSSYKE
                     AIRAYIDNTFTDDKIADWDKRLSKVFNRGFWDGYYLGQRLGEWSANYGSQATHRKVYI
                     GKVTNYFKNIGVAEILVETQSIETGNEILITGETTGAYESIADDIRVNLISVNQAKKG
                     DLFSIKTTDIVRRNDKVFKIVPAKNVSITQ"
     gene            complement(14000..15178)
                     /locus_tag="PLJ82_02295"
     CDS             complement(14000..15178)
                     /locus_tag="PLJ82_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOR40373.1"
                     /translation="MLLYTDSFKKELLLRWSKIQRKLKELDVEACLISTNVNSWYAVG
                     QIIIGYVYLRQEGEPLVFVRRPSDITGNNVYPIRKIEQIPDIFADLQMELPQSILLEG
                     DEISYAEWMRYKAVFNTERCINGTHALRQVRSIKTDYEVAELRESARLQSIAYKDIPL
                     LYRPGMTDHEFTVEVERLFRLRGNLGLFRIFGSSMEAFMGSVLVGENAAATSPYDFAL
                     GGKGMHSSLPFGHSGLLLEKGKSIMVDINGNFNGYIADQTRTFSIGELSEEAYYAHKV
                     SLEIQQVLAEAGKPGTVCEELYQRSLQIVKKYNLEDCYMGIEQQAKFVGHGVGLVINE
                     LPVLCDRNKELLETNMTIALEPKFIIKGVGAVGTENTYVVTDKGMEKLTFAPEDIIEL
                     "
     gene            complement(15186..17771)
                     /gene="mutS"
                     /locus_tag="PLJ82_02300"
     CDS             complement(15186..17771)
                     /gene="mutS"
                     /locus_tag="PLJ82_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOR40374.1"
                     /translation="MKQYNQVKAKHPDAVLLFRVGDFYETFSDDAIRVSEILGITLTR
                     RANGAASYVELAGFPHHALDTYLPKLVRAGLRVAICEQLEDPKTAKGIVKRGITELVT
                     PGVSYNETTLNFKENTFVAAVHFEKSRVGVAFLDISTGEFLTAEGSSEYVDKLLTSFS
                     PKEVLYERGKTSLFEQSFGTHFLSFTLEDWVFVSDTANERLQKQFQTKNLKGFGVVDL
                     PLGIIASGAILHYLEFTNHLQTAHITHLRRIEEERFVWLDRFTIRNLELYQAMHDGGK
                     SLLDVLDKTLTPMGARMLKRWLAFPLKNKAAIEHRLSIVSYFFKDKDIQQVLYQQLSL
                     IGDLERLVSKIAVGRINPRELVQLKIALTAVETIKNSSTLVDNEALVSIAERLQSCTR
                     MRDKIEQTIASDAPMLLHKGGVIKQGIHPELDEYRRLAYSGKDYLLQIQQRESEATNI
                     PSLKISYNNVFGYYIEVRNTHKDKVPSNWIRKQTLVSAERYITEELKEYEEKIVGAEE
                     KIAQIEGQLFNELVVSLCDYISALQLNASLIAELDCLLSFANVSANNSYIPPVITETD
                     VLSIKQGRHPVIEKQLPIGESYIANDVYLDTNTQQIMMITGPNMAGKSALLRQTALIV
                     LMAQIGCFIPAESAEIGIVDKIFTRVGASDNISMGESTFMVEMSEASSILNNVSPKSL
                     ILFDELGRGTSTYDGISIAWAIVEYLHEYPQAKAKTLFATHYHELNEMEKTFSRIKNF
                     NVSVKEMDNKIIFMRKLVPGGTEHSFGIHVAKMAGMPKSVVKRSNEILAQLEKDNRKT
                     GQPQKHIDTVAHQREGLQMSFFQLDDPVLIQIRYEIKNVDVNNLTPLQALNKLNEIKK
                     IITGK"
     gene            complement(18046..18315)
                     /gene="rpsO"
                     /locus_tag="PLJ82_02305"
     CDS             complement(18046..18315)
                     /gene="rpsO"
                     /locus_tag="PLJ82_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR40375.1"
                     /translation="MQLTKEQKQEIFEKYGKSNTDTGSSEGQIALFSARIAHLTEHMK
                     SNHKDHSTERALVMLVGKRRRLLDYLKAKDIERYRAIIKELGIRK"
     gene            18493..19008
                     /locus_tag="PLJ82_02310"
                     /pseudo
     CDS             18493..19008
                     /locus_tag="PLJ82_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
BASE COUNT         6202 a         3730 c         3431 g         5687 t
ORIGIN      
        1 aattggttga aaacggaatt aattgaaaca atttaaatga gaaaataatg ttgctctatt
       61 cctttaaata cacccagtca ggacggactt ctcaattaag agtgttcgga agacttttct
      121 gagaaccaac gacttggggc attgcaagaa cacataaagt aaaaaaatct gatgacaaac
      181 ctcgtcattt aattttttgc attacctttg tagcgaaaat ttacatccca tgaacaaagc
      241 acaagcacaa acacatgtta ttcgttttaa gcgatactcg cgtaaggcgt atgctgcttt
      301 ttgttcgatg cacaagcaca ttaccattgg taaacttgcc acagcacttt gcgacaaaca
      361 attggctaaa agcaaactag cacatacaaa ttcggctgat tgtttggctt tttatacacc
      421 agtattttct gatgaatcaa ttgtggacaa tgagatgttt gcttttgatg tgttgccgtt
      481 ggggtgtgtt gctgttatcc ctaccactta ttctactact gagggtcagg aagctcggat
      541 ttcctgctcg gattgtttat acacgctgtt gttgtacctg atggtatttt caacagcgtt
      601 tttgtttgca catactttgt attcacttaa cccttttatg atatgaacaa gaaaaaaatt
      661 ctttccttgg cattgcttgc caccctattg cctcaacttg gaatgatggc acaaagcaat
      721 agttctaata cgcaacagct aactgaaatc agtgttcacg ctcagcccac caaggtgtat
      781 tcagaccatc tgcgagtagt gatgagtata gacaagagca caatagctca aatgcctgta
      841 caaaccattc aagagctact taacaacctg cccggactgg acgttcgcca gcgtggtggt
      901 agtggtgtgc aagccgatat cagtatgcga ggaggcactt tcgaccaagt gttaatctta
      961 ctcaatggag tagctatcag cgacccacaa acgggtcatc atacgcttaa cctgcctatc
     1021 gatttaaata tggtagagcg agtggaaata ttgcaaggcg gtgccatcag tctctttgga
     1081 accaatgccc taagtggtgc tatcaatatt gttaccaacg aaaaagctga tgatgccctg
     1141 aaagtaagcc tcactggtgg ttcgtttggc ttatttaacc cctcagtgtc aagtacattt
     1201 gcaagcaaca aatggcgatg ggcaatatcc gcttcgcaca acgagtcagc gggctatacc
     1261 gagaataccg actataggta tacacaaggt tttgttcaag cacaatttca agctacggaa
     1321 caagatatat ggaaatggca aataggcgtg caagacaaag cttttggagc aaattcattt
     1381 tattcggtaa attatccgaa tcaatttgaa aaaacaaaaa cccttatcag cagtatcagt
     1441 tgggataaac gcattaattc cctgttgtgg caatcaactg cctattacag agggcatttc
     1501 gatcagtttg aactatttcg caattatgaa aacgccgcaa cttggtacac gtttcacaat
     1561 tatcacctca gtcatgtaag tggcatcaac tcaaagctgt cgtggtttac tagtttagga
     1621 aaatcaagca tcggcataga gggtcgcaac gaacatatta acagcaatgt gttaggagaa
     1681 ctgaaagcaa caccataagc cattccgttt gccaaaaata ccaatacgca gtttttatac
     1741 gaaaaaaacc gtaccaatgt gcaatatttt gccgaacaaa caatttactt acaggcattt
     1801 acagcttctg ttggtgtatc gggcaattat aatacgcaat ttggcaacag gtatagttac
     1861 gggcttaaca cttcgtatgc attcaatacg tatgctcagg tatttgccaa tatcaataaa
     1921 tcgttacgac tacctacctt tactgattta tactacaaaa gtgctacgca agagagtaac
     1981 ccgagcttaa aacctgaaga agctatcacc gcagaaatag gagccaaata caccaaacag
     2041 gcatggcaat ggaatgcaaa cgcattttac cgtatagcca acaatagtat cgactgggta
     2101 cgctcgctcg aaacagaaaa atggaaaagc atgaaccata ctcgcattga tgccatggga
     2161 ggagaaatag cggcagctta tacaggtggt ttttgggcaa aaaacataca agcatcgtat
     2221 gcctacgttc tgctcaacaa agatgcgggc gagctaattt caaaatatgc tctcgactac
     2281 ctgcaacaca aaattaacct gcaatatgtg catggaataa gcaagcaggt agatgccagt
     2341 tgggtagtaa gctaccaaga ccgtaaagga aattatgtaa acaaaaatgg aaacaaagta
     2401 gcttatactc catttgccac catcgacact cgcatcaact ggcaacaaaa taacacaaaa
     2461 atgtatgccg aaatatccaa tatcctcaac caacattatt acgactatgg cggcgtgccc
     2521 atcgctggac gtatgttgaa agttggtatt gcggtgcagt tgtaacaggt taactaattc
     2581 tttctctaaa ccttgctctt gaaaaaagca aggttttttt tgggataata aaacgtgggt
     2641 attcagtgaa aatgagctat ttaacacatt aatcgaaaga catattattt tttttcatac
     2701 atttgtgggt taccctaata gcatacttat atgaataagc ctactatctt ccactttttc
     2761 cgcccactga ttacattatt catcctgcta catgtagctc tggtttctat attagcacaa
     2821 actgttgact taaaaactac cactttcaat gctgaatggc ttagttgtga tacaaatgga
     2881 ccctctgatg aggaagggca aatgaacaat attgtttccc ttattcaaac gataaatccc
     2941 gatatcatcg ccttgcaaga agtaggtaca agtacaacct atgcaaccat cgataccttg
     3001 gtaaaacatt tggaataaac gaatgggcag gagctatcgt ggctactact cacactaatt
     3061 gcggtcaaaa tcaaggcatt atttacaaaa aattccacct acaacttgtt aattcttccc
     3121 tactgagtaa cggggcaaat agccaaggaa acagttacta ctataattgg actaatggtc
     3181 gttatcctgt tatacatgat ttcgtattga ctattggctc aacaaatatt ccgttttcca
     3241 tcatcaatat acatgccaaa gccatgagtg atgaaaccag ctatagccga aggcttggag
     3301 cttccgaagg gctaaaaggg atactagatg gtacgaacta caatacaagc aaaattataa
     3361 ttacaggcga tttcaacgac tatttaatag gttcgcaaac ttcttcgtac tccaattctc
     3421 cgtacaaaaa ctttatggac gatacggaaa attaccaaag tcttacctct tccttaaccg
     3481 atccttatta cagtagccct gtgattgaca atatcattat tacaaatgaa ctggctagcc
     3541 atcatgttgc gaatagcacc atacgcgaga ctagtgccac caatacgata agcaattacc
     3601 gcaacacaac ctccgatcat acgccaataa gtgcactgtt ccgtttcaca agcaacaatc
     3661 caactgactg ccaagcgata gattattcgg agagctttgc aagtagcttg ggtgatttta
     3721 tcccctattc tgtagaaggc acacaaacat ggcattggcg aaatatttac ggagcatacg
     3781 tgtcaggata tgccagtagc acgaataatc cgaacgaaga ttggcttatt tcgcctgctt
     3841 ttgatttatc cgacaaagaa tcgggctcct tgtcgtttga acatgccctt aactatgctc
     3901 aagaagcaaa cgagcgagcc agcaatcaca ccttatggat ttcaacaaac tataccgaag
     3961 gagacccaaa aaatgccaat tggacacagc taagtattcc cacaatgcca ttgggcacta
     4021 gctggacata cgtaaactct ggtagcataa acctgcctag ccaatggatg aaagaaaacg
     4081 tgcgttttgc gtttaaatat atggcaacat caagcatggc tggtacgtgg gaaataaaaa
     4141 atctgctatt caatgctact tgcgaaccag tagcaatacc ctctatatca aacgataaca
     4201 aggtagtatt tgcacaaaat cgcaacatta ctgttttgtt ggaacacgaa acatccatca
     4261 gaatattcaa cattcttgga aacacattgt ataacgacac ccgcagcgaa aacagcgtat
     4321 ttccagtttc gcaagcaggt atctaccttg tgtgtatagg aaacgaatgt tacaaaattg
     4381 cggtaagata aagaacaaat agtactagtt ctactttttt aaccatttct ctacttttct
     4441 atttctgtta aataaaatgt tttgcgtacc tttgttgtta cgaaaaaaaa tactgtatga
     4501 aaactcctat cctttctata ctccttcttg ttggttggtt tgccactttt gcacaaacat
     4561 cagcccaatt cgaaatgcaa aaactcaact tttcgtatgg agctattacc aatttttatg
     4621 tagataaagt tgaccctagt aaactaaccg aatgggctat caaaggcatg cttgatgagc
     4681 tagaccctca ttctagttat ctatcagcag atgaggtaaa agaatcgaca gaagcacttc
     4741 aaggtagttt tgatggcatt ggcgtatcgt ttaatatggt agaagacacc ttagtagtaa
     4801 tccaatcaat aactggcgga ccatccgaaa aactaggcat attgcctggc gataaaataa
     4861 tcttggttaa cgataccacg attgctggcg taaaaatgaa aactagcgac attaccaaac
     4921 gcttaaaagg tcccaaagga acacaagtaa aagtaactat tttgcgaaac aacgaatcaa
     4981 ttccgtttac tatcattcgc gaccgtattc caatttttag tgtagatgct ttttatatgg
     5041 tagacaataa aattggatat atcaaagtca accgttttgc cagcaccacc catcaagaag
     5101 tggtagatgg cataaaaaat ttgaaaaaga aaggcatgga gtcgcttatc attgatttac
     5161 aaggaaatgg aggtggctat ttgcaaagtg cgattgaaat agtagatgaa ctgctcccaa
     5221 ataaacgtac agttgtgtat actgaaggag ccaatcatgc caaacaaaca atgacaactc
     5281 gccgtaatgg acttatggaa agtggcaaag tagtcgtatt agtagatgaa tattctgctt
     5341 cggctagcga aattgtagca ggagctttgc aagattggga cagaggagta gtgattggca
     5401 gacgcacgtt tggcaaagga ttggtacaac gtccaatacc tatgcccgat ggttcggaaa
     5461 ttaggcttac cgtagctcgt tactacaccc cttctggacg cagcattcaa cgtccttacg
     5521 aaaaaggaaa agaaaaatac gaggaagata ttcttgatcg ctataaacat ggggagtttg
     5581 tatcggctga cagcattaca tttgccgatt cacttacata caaaaccctt gttacccaac
     5641 gtactgtata tggcggaggt ggcattatgc ctgatatttt tgtgcctgct gacacaacta
     5701 aatataccac cttgcaccgt caactgatac gcaatcatat cattacaaaa ataagtattg
     5761 tatatgctga aaaaaataga gctcttttat caaaacaatt tagtactttt gaagcgttta
     5821 aattgaaatt tgacacacaa gaagttctct cccatataaa acaagaaacc ttagcaaaag
     5881 aaatcgcgtt tacggaagaa gaatggaacg aaacgaaaga cttgttagca atacaggcaa
     5941 aagcaatcat tgcacgcaat ctttggtcga caaacgaata ttttgaaata ataaacgaaa
     6001 caaacgattt tgtaaaaaaa gccatcgaag tactaaaaag cgaatcgata tattacaaca
     6061 ccctgcaata agaataagaa cgtaaactga tgaaaaacaa gaattacgcc tccgatatta
     6121 agactgttat cgacgagtta acaggcttat gcccaacaga aaatatgttt caccccaaca
     6181 aagagggcga accatttccg tcacgggcaa taatgaaaga tagtatcaaa ctaacacgtt
     6241 ctattttgtt tccaggtttt ttcggcaatg cgagtgtcaa caaaacaacc ttgccatacc
     6301 atattggtgt aaatgtggaa aaattattta ctatgctttg ggaacaagta tatgccggtt
     6361 tgtgttttga ttgtaaagaa gaagacattg acttggaagc taaacgcgaa aaagcattta
     6421 cgattaccct ttcgtttgta aaacaattac cccgtataaa agaagtgttg cgtacagacg
     6481 tagaggcaat gtataaaatg gatccagctg ctaaaagtta cggtgaaatc attatttcgt
     6541 atccaggact tagggctatt accaattacc gaattgcaca cgaattacac aaactacaaa
     6601 cacctatcat tccacgcata attaccgaaa tggctcatgc cgaaacaggt attgatattc
     6661 accctggggc acgtattggc gagtcatttg ccattgacca cggcacagga gtggtaattg
     6721 gcgaaacatg tattattgga aacaatgtgc gtatttatca aggggtaacg ctgggtgcta
     6781 aaagttttcc tgttgatgaa aataatatcc caattaaagg catagacaga catcccatta
     6841 tcaaagacaa tgtgattatt tatgccagtg ccactatttt gggtagaatc atcattggcg
     6901 aaaatgctgt aataggcgga aatacatgga ttaccgaaga tgtgaaacca aacgaaaaag
     6961 tatttgtaaa ataagccata cccttaaaaa aagtgtaata ggctgaccca ttttatgttg
     7021 agtcagccta ttttttatgt ctacaagaaa taatctaggt ttgttaatac ggaattttgt
     7081 ctgttttctg tttccatgcc ttaaatggag ctttgccttc ttgtaccaat agaggtactt
     7141 gtagtacttt tctgttttct ttgggcaagg caaattcttc gtaaataaaa gcatcatcaa
     7201 aaccaacctc cgcagcatcg tgtttagtag ccgaaacata tactttgtcg atacgtgccc
     7261 aataaatggc agccaaacac atagggcaag gttcgcaact ggcatacaac tcacagcctg
     7321 ataaatcgaa cgtttttaaa tgagcacaag cctgcctaat tgcatttact tcggcatgag
     7381 cagtaggatc gttggtcgaa gtaacagaat tggctgcttg acctacaatt ttaccctctt
     7441 ttactactac ggcaccaaag ggtccgcctt tgccctgttt tacattctct atcgctaaat
     7501 ctatagcaag ttgcataaaa ctagtacttt ccatcttcat tctttattcg taaaacctct
     7561 atgttcaact atctcttacg tgtaaagata caaaatacta gcgaatgata acacacttgt
     7621 gtaataaaaa atacagaagc agtataaaat atggctcgtt taccaaagat atatcgtatt
     7681 tttgcatacg aatggaaaca aatacagaac ttgaattagc gtggcgatgt gtagtgcaaa
     7741 caggcactca cttgttttta acaggtaaag cgggtacagg aaaaaccact ttcttgcatc
     7801 aacttaaaac aacctcgccc aaacgcatgg tagtacttgc tcctaccggc attgcggcaa
     7861 taaatgcagg aggcgttact atccattcat tttttcaact accttttgct ccctatattc
     7921 ctggtacaga atttgccaca gatacaaaat accaatttaa caaagaaaaa ataaacatca
     7981 tacgcagttt agatttattg gtaatagatg aggtgagcat ggtacgtgcc gatttgttgg
     8041 acagtgtaga tgccatattg agaagataca gacgcacaac cgtgcctttt ggaggcgtac
     8101 aactgttact tattggcgat ttgcaccagc tatctcctgt aataaaagaa gaagaaagaa
     8161 gattgctaga agcgtattac cccagtagct atttttttaa cagcaaagcc ttgcaaaaaa
     8221 cagcttatct taccatcgaa ctaaaacagg tatatcgcca aagcgagaaa cattttttgg
     8281 atttattaaa ccaaatacgc gacaacaaag ctacctccac gacgcttgaa gaattaaaca
     8341 aacgctatat tccagatttt attcctccag caggcgaaga gtatattcgc ttaactacac
     8401 acaattacca agcacaaaca atcaacgacc aacatttagc aaaaattagc agtcctgcat
     8461 tttcgtataa agcacaagta aatggaaatt tcccgcaaca tgcttatcct gctgacgagt
     8521 tgttgactat caaagaggga gctcaaatta tgtttatcaa aaacgatgca agctttccaa
     8581 aacgctttta caacggaaaa ataggcaagg taacacatat cgatgagcat gttatcgaag
     8641 tgcaaacaaa cgatgacccc gaaccattta gtttgcaaaa agccgtatgg acaaatgcaa
     8701 aatacgtatt gaacaaagaa actaatgaga ttagcgaaga tatagaaggc acttttgagc
     8761 aatatcctat tcgcttggct tgggcaatca cgattcacaa aagccaagga ctaacatttg
     8821 ataaagcgat tatagatatt gcttcctcgt ttgctcacgg acaaacatac gtggcactta
     8881 gtcgttgtcg cacattaaat ggaatggtgc ttaattctcc tatttcgcat cgagccataa
     8941 taaaagacgc tgtaatacat gattttgtgc aacaatcagc cacagatgct ccagatgaga
     9001 gtaaaatagc tgtattagaa cgagcgtatt tttcatattt agtaacagaa atggtgcaat
     9061 tttctgacat caccaaactt cttgtgcgtg gtagttactt atttaaagaa tctccactca
     9121 tcggtatgta tccttcaatt catgaacaac taaaacaatc cattgagcac atacaacaag
     9181 atattgaaca agtaagtagt cgttttcttt ctgttttctt ttcgatgttt cgcaacacac
     9241 ccgactatca agacaatgca gcattacaag aacgcataca gtcggcagca agttattttt
     9301 tggttcaact caacacattg gtagcaccac ttattgctgc gataaaagta gatgtagata
     9361 acaaagagac agaaaaacaa gtaaacgatt tacatgctca acttagtgaa actctacaaa
     9421 ttcataccgc ttgtttaagc agttgtaaaa acaatgggtt tagcgttagt gcctatcaaa
     9481 aagccaaatt agaagctgcc attgaacata catctacaaa aaaacgtact gccaaagcat
     9541 ctgagaaaca gaaagtatcc atcaatagtg gagaaactga acatgcacaa ctatacgagc
     9601 acttacgagc ttggcgacta caagaagctt ctgaaagaaa gctacctgcc tatgccattt
     9661 tacaccaaaa agctctgttg ggtattgtgc aacatttacc cttgtcgcca acagaattac
     9721 ttcaaattcc tcaccttggc aaacgtactg ttacaaaatt tggcgatgcg ataattgaaa
     9781 ttgtacgcga atttgtgcaa tcaaaagaaa acgacaaaac taactcataa ccaaacatat
     9841 acgtaaatta attttttcgt tcaaaaatta tctgtgcaat tatttgtggc ttttttctgc
     9901 cgaataaacg ccaattaaat ccgaatgcgt aatataccgc aaattaacac tatctttgca
     9961 caccatttta tatatactat atgaagaatt tacatattgg agacttagtt attcccgttc
    10021 caattataca aggaggtatg ggcgttggta tttcattatc aggcttagcc tcagcagtag
    10081 ctaatcaagg aggagtaggc gttatgtctg ctgcaggact aggattgtta taccgacatt
    10141 tatcatcaga ttatcccgaa gcatgtatat taggattgcg tgaagagata aaaaaagcac
    10201 gagacaaaac aaaaggaatt attggggtaa atatcatggt cgccatgacc aattttgctg
    10261 atatggttaa aacatcaata gccgaaaaag tagatatcat tttctctggt gcaggattac
    10321 ccctcgattt accttcattt ttacaaaaag atagcaccac aaaattagtt cccatcgttt
    10381 cgtcagctag agcagccaaa ttaatttgcg acaaatggaa agctgcatac aactatttgc
    10441 cagatgctgt ggtggtagaa ggtccaaaag cagggggaca tttaggctat aaagaatctc
    10501 aactttctga cgatgcttac agattggaga ctattattcc agaagtagta gctacaatta
    10561 aagaaataga aacaacacac gactgcaaaa tttctgttat tgctgctggc ggtatttaca
    10621 caggtgcaga catgtatgac atcatgcaat taggtgctga tggggttcaa ctaggaacac
    10681 gttttgtaac cacgaccgaa tgtgatgctt cagaaatttt taaacaagcg tatattgatg
    10741 ctgccgaagc ggatgtagaa ataataaaaa gtccagtggg aatgcctggt agagcgatct
    10801 ttagcgattt tttacaaaaa gtgaaagatg gcgaaaaaca gccgaaaagt tgtcctttcc
    10861 attgtattaa aacctgccaa atagaaacaa gcccttattg tattatgttg gctctgttta
    10921 atgcatacaa gggcaatatg accatgggtt acgcatttgc tggcacgaat gcacacttgg
    10981 ctacagcaat tgttaccgta aaagatacca tggatgctat ccaagaagaa tacaaagcta
    11041 gagaaaaaga gctagtttaa taaaaaattg tatttttgta agcaaatact actaccacaa
    11101 cagttaacaa aaacaactag tgtggtaaaa gtggattcat ttcgttaaac ctactataac
    11161 gtaaatatgg aagaacttac attaactaca ccttcattgc ttttttctgc agtatcgtta
    11221 ttgctgttgg catataccaa tcgttttttg tcgtatgccc aagtggtacg aacgctgaaa
    11281 gaaaaataca ccaaagatca ttcagccatc acgggggcac aaattaacaa cttacgtaaa
    11341 cgattatacc tcacacgagc gatgcaagtg caaggcattg taagtttatt tttatgcgtt
    11401 gttactatgc tgctgatata catacagtta caactcatag ctgcttatgt gtttggggta
    11461 gctttatttt tactcatcct ctctttagga acttcgattt gggaaatata catatccgtt
    11521 aaagctctcg aaatctacct aagcgacata accaacgaat tggataacaa gaagtaatgt
    11581 ttaaaaaatt agtccataga ctataataag tcatttttat ttacttgttt ttcaataaac
    11641 tattgctact tttgtgcttt gtatttcaat atatttctat cagccacatt tctaataaaa
    11701 acacacatga atctgcttaa aaacaacatc gctactttct ttaaaggttt tgctatgggt
    11761 actgctaatg ttataccagg ggtatcagga ggcacaatag ctcttatatt gggtattttt
    11821 gaacgaatta ttgatagctt aaaatgtttc gatggcgagg ctattaaatt attgataaag
    11881 ggtaaattta aagcatttgc tacacataca gacttgggtt ttttatcagt agtactgtta
    11941 ggagtaattg tcagtatctt tacagttgca aaactgttaa gctttctttt tcttaattac
    12001 cccattttta tttggtcgta tttttttggt ttaatcattg cttcaatttg gctggtgggt
    12061 aaaactattt ctagctacaa tacctctgta attttatttc ttattcttgg cacagctatt
    12121 gccgcctcaa taacaatatt aaatccagca agtgaaaatg attccttttt ttatcttgtt
    12181 atttgtggta tcgtagcagt atgtagtatg attttaccgg gcatatcagg tagttttgtc
    12241 ttggtactga tgggaaatta cgaacttgtt ttacgctcga tcaatgaatt caatattacc
    12301 aacttggtcg ctgtggctat tggttgtgca ataggacttc ctgctttttc gcatttgctt
    12361 tcttggctat acaaaaccta taagaaccaa accattgcaa tgcttactgg cttcatatta
    12421 ggttctttag ccgttatttg gccatggaaa tactcttatg ataccgttgg aaatctagtt
    12481 gaagccaata aatttggagc attggtgaat acagatgtaa aaataataag ctatgaacgt
    12541 attttacctc acatagatgg attgtttttc atcgctatta ttatcgctat tcttggtttc
    12601 ctatctatct acttattgga aaaaaaggct atacatatga aagaggaata gtattttcat
    12661 attcttgtat tgtatctttt taacctatca tccaccatta attaatacat tttgagtatt
    12721 tttgcagaca tttacaatac accaatggct tctttgtctg tacatgatat tgaaataatg
    12781 gctcccgcag gttcgtggga aagcttgtat gcagctctac atgcaggagc tgattctgta
    12841 tattttggta tagaaaacct taatatgcgt agccattcgt caaataattt taccattaac
    12901 gatttacgag agattgttcg cttgtgccac gagcacaagg taaaatcgta ccttaccatc
    12961 aatacaatac tctacgacga agatatcgaa ttgatgcacc aaatagtaga tactgcacaa
    13021 gaagaaggta tttcagctat catagctgct gatgtagccg tgttaacgta tgcacgtagc
    13081 aaaggagtgg aagtacatct ttcgactcaa ctcaacatct caaatgtgga tgcattgata
    13141 ttttatgctc aatttgcaga tgtagtcgta cttgcacggg agctcacgat gaatcaggta
    13201 gcacatattt ttaaaaccat taacgacaga aatatatgtg gaccaagtgg gaaccgaata
    13261 cgtatcgaaa tgttttgtca tggagctttg tgtatggctg tttcgggcaa atgttacttg
    13321 agtttacacg agatgaacaa atctgcaaat cgtggatcat gcatgcaaat atgccgaagg
    13381 agctacgaag ttaaagataa agagctcggc attgaattag aagttgacaa cgaatatata
    13441 atgtcgccca aagacctcaa aacaattcat tttgtaaata aaatgattga tgctggtgtg
    13501 cgtgtattca aaattgaggg cagagctcgt agtgcagaat atgtaaaagc taccgtaagt
    13561 agctacaagg aagccatacg tgcgtatata gacaacacgt ttacagatga taaaattgct
    13621 gattgggaca aacgtttaag caaagtattt aatcggggat tttgggatgg atattactta
    13681 ggtcaacgac taggcgaatg gagtgccaac tatggttcgc aagctacaca ccgaaaagta
    13741 tatattggca aagttaccaa ttattttaaa aatattggag ttgccgaaat cttagtagaa
    13801 acacaatcta tcgaaacagg gaacgaaatt ctaataacag gcgaaacaac aggagcctac
    13861 gaaagcattg ctgatgatat ccgtgtcaat ttaatatccg taaatcaagc aaaaaaagga
    13921 gacctctttt ctatcaaaac aactgatatc gtacgtagaa acgataaagt ttttaaaatc
    13981 gttcctgcaa aaaatgtttc tataactcaa taatgtcttc aggagcaaat gtcaattttt
    14041 ccatcccttt atcagttacc acataggtat tttctgtgcc tacagctcct actcctttga
    14101 taataaactt aggttcaaga gcaatcgtca tgttagtctc taaaagttct ttgttccgat
    14161 cacataacac aggcaactca ttgataacca accctacacc atgcccaaca aatttggctt
    14221 gttgttctat ccccatataa caatcttcta aattgtattt tttgacaatt tgtaaggatc
    14281 tttgatacag ctcttcgcac acagtacctg gtttgcctgc ttctgccaac acttgttgta
    14341 tctcaagaga cactttgtga gcatagtaag cttcttcgct tagttctcca atagaaaacg
    14401 tacgtgtttg gtcagcgata tacccgttaa aattgccatt gatatctacc attatactct
    14461 tccctttttc gagtaataga ccactatgac caaaaggcaa tgaactgtgc atacctttac
    14521 ctccaagggc aaaatcgtat ggtgaggttg ctgctgcatt ttcacctaca agcacacttc
    14581 ccataaaggc ttccatggaa gaaccaaaaa tacgaaacaa ccctaaattt cctctcaaac
    14641 ggaacaaccg ttctacctca acggtaaatt catggtctgt catgcctggg cgatacaaca
    14701 aaggaatatc tttataggct atggattgca aacgagcaga ctctcttaat tctgcaacct
    14761 catagtctgt ttttatgctt cgtacttgac gtaaagcatg tgttccattt atgcatcttt
    14821 cggtattaaa tacggcttta taccgcatcc attcagcata ggaaatttca tccccttcaa
    14881 gcaaaataga ttgagggagt tccatttgta aatcagcaaa gatatccggt atttgctcta
    14941 tttttcgtat tggatacaca ttgttgccag taatatcaga aggtcgcctt acaaaaacca
    15001 aaggttcgcc ttcttgacga agatacacat acccaataat gatttgtcca actgcatacc
    15061 aactattgac attggtagaa ataagacaag cttctacatc aagctctttt aatttacgtt
    15121 ggatttttga ccagcgtagg agtaattctt ttttaaacga atctgtatac agtaacataa
    15181 tagtgttatt ttcccgttat tatttttttt atctcattca atttattcaa tgcttgcaag
    15241 ggcgttaaat tatttacatc aacgttttta atctcatacc tgatttgtat caatacgggg
    15301 tcgtctaact ggaaaaaact catctgcaag ccttcacgtt gatgagccac tgtatcaata
    15361 tgcttttgtg gttgtcctgt tttgcgatta tctttttcta attgagctaa aatttcgtta
    15421 gagcgtttta ccacactctt tggcatacca gccatttttg ctacatgaat accaaaacta
    15481 tgttctgtgc ctcctggcac taatttacgc ataaaaataa tcttgttatc catttctttt
    15541 acagaaacgt tgaagttttt tattcgcgaa aaagtctttt ccatctcatt taattcgtga
    15601 taatgggtag caaataatgt tttggctttt gcttgaggat actcgtgcaa gtattccact
    15661 atagcccaag caatagaaat accatcgtag gtactggttc cccttcccag ctcatcgaat
    15721 aaaattaaac ttttgggcga tacgttattt agaatactgg atgcttcgct catttcaacc
    15781 ataaacgtag attcgcccat tgagatatta tcgctagcac caactctagt aaaaatttta
    15841 tctacaatac caatttcggc actctcggca gggataaaac aacctatttg tgccattaat
    15901 acaataagag ctgtttgtct taataaagct gatttaccag ccatattggg tccagtaatc
    15961 atcattatct gttgggtatt ggtatctaaa tacacatcat tggcaatata ggactcccca
    16021 ataggaagtt gtttttcgat aacgggatga cgcccctgtt tgattgacaa cacgtccgtt
    16081 tcagtaataa ctggaggaat ataggagtta ttagctgaca cattcgcaaa agacaacaag
    16141 caatctaatt ctgcaattaa ggaagcattc agttgcaagg cactaatata atcgcacaac
    16201 gatacaacta actcattgaa aagttgtcct tctatctggg caattttttc ttctgccccc
    16261 acaatctttt cttcgtattc tttcaactct tcagttatat agcgttctgc actgactaag
    16321 gtttgcttac gaatccagtt tgagggaact ttatctttat gcgtattacg tacttcaata
    16381 tagtagccaa atacattgtt ataacttatt tttaggctag gtatattggt tgcttcactt
    16441 tctctctgtt gaatttgtaa taaataatct ttacccgaat aagctaaacg tctatattca
    16501 tccaattcag ggtgaattcc ttgtttaatt acaccccctt tatgtagtaa cataggagca
    16561 tccgaagcaa ttgtttgttc aattttatct cgcattcgtg tacacgactg caaacgttcg
    16621 gctatagata ctaatgcctc attatcaaca agtgtacttg aatttttaat tgtttcaaca
    16681 gctgttaacg caattttaag ctgcaccaat tcacgaggat tgatgcgtcc aacggctatt
    16741 ttagacacca aacgttctaa atctccaatt aacgaaagtt gttgatacaa tacctgttga
    16801 atatctttgt ctttaaaaaa ataactgaca atactcaatc gatgctctat ggctgcttta
    16861 ttttttagcg gaaaagctaa ccatcttttc aacatacgag cacccatagg agttaaggtt
    16921 ttatctagca catccaataa tgattttcca ccatcgtgca tggcttgata caattcgagg
    16981 ttacgaattg taaatctatc taaccaaaca aaacgttctt cctctattct acgcaaatga
    17041 gtaatgtgag ccgtttgtaa atgattggta aactccaaat agtgtaaaat agctcccgaa
    17101 gcaataatcc ctaaaggtaa atcaactacc ccaaaacctt ttagattttt tgtttgaaat
    17161 tgtttttgta atcgttcgtt tgctgtgtct gatacaaata cccagtcttc taatgtaaat
    17221 gaaagaaaat gggtaccaaa agattgttca aaaagacttg tttttccgcg ttcgtacaaa
    17281 acttcttttg gagaaaaact cgttagaagc ttatctacat attccgagga tccttctgcc
    17341 gttaaaaact ctcccgtaga gatgtctaaa aaggcaacgc caacacgtga tttctcaaaa
    17401 tgaacagcag ctacaaatgt attttcttta aaattgagcg tcgtctcgtt atacgaaact
    17461 cctggcgtta ccaattcagt aatgcctctt ttgacaatcc ctttggcagt ttttggatct
    17521 tctaactgct cacaaatagc aacgcgcaaa ccagctcgta ctaattttgg taaatacgta
    17581 tctaaggcat ggtgcggaaa ccctgctaat tctacataac tagcagctcc gttagcacga
    17641 cgtgtaagcg taatacctaa aatttcagaa acacgtatag cgtcatcaga aaatgtttcg
    17701 taaaaatcgc caacacgaaa caacaacaca gcatcaggat gtttcgcttt cacctgatta
    17761 tattgtttca ttaaaggggt ttctacaact tttgccacaa aatcttacat ttgaaataca
    17821 aaacacaaag ataaaaaata tcaaggaaga aatgttattt tagtaaaaca aatttaatta
    17881 agaacgtata atgatactga taaatccatt acacttagtc caatcaccaa atattaacgg
    17941 tcttattatc attttcagtc aagttgaatt cctcaaaaaa cagagaacaa cgcataaaaa
    18001 aagtccgaat cttcattcgg actttttttg ttaaaaggac atcaattact ttctgatacc
    18061 taattctttg ataatggcac gataacgttc aatatctttt gcttttagat agtcaagcaa
    18121 acgacgacgt tttcctacca acattactaa agcacgttct gtgctatgat ctttgtgatt
    18181 tgacttcatg tgttcagtca aatgagctat acgggctgaa aataatgcta tctggccttc
    18241 tgatgaacca gtatcagtat tcgactttcc gtatttctca aagatttctt gtttttgttc
    18301 ttttgttaat tgcatacaat ttattctaag agattattaa aaaatgtccg caaagataca
    18361 taatagtttt ttagttttca acaatatttc ttaaaaactt tcagaatcat tcatttccta
    18421 cttgtatttg ggtaaaattt agtacttttg tgaggctttt tttaagggaa atcagaacta
    18481 atcaggaaaa aaatgaaatc atttaaaacc atgaacaccc tatttgggtg gataagtttc
    18541 ttaattgcat gggtggtgta tgcccttact attgaaccta cagctagctt ttgggactgt
    18601 ggtgaattta taacctccgc ctttaaactg caagtgggac acccacctgg ggctcctttc
    18661 tttaatttaa caggtaattt tttcacgcta tttgcaaaag atccttctca agtagccatc
    18721 atggtaaacc taatgtcagc aacattaagt gcgtttacta ttttattttt gttttggaca
    18781 attacgcata ttgcaaaaaa aatcattgtt caaaatgggt cagaaataac actaagtcaa
    18841 tctattacaa taatcggaag cggattaatc ggatctctag tctatgcttt ctcggacaca
    18901 ttttggttct cagccactga agccgaagta tatgcttatt cttccttttt tacagctatt
    18961 gttttttggc ttatattaaa atgggaagac caacacgata aagcaggtta agataaatac
    19021 ctaattctta tcgcttactt aatgggctta
//
LOCUS       DAOSRP010000020        17919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3273_length_17919_cov_5.949563, whole genome shotgun sequence.
ACCESSION   DAOSRP010000020 DAOSRP010000000
VERSION     DAOSRP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_17919_cov_5.949563"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(505..903)
                     /locus_tag="PLJ82_02315"
     CDS             complement(505..903)
                     /locus_tag="PLJ82_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40376.1"
                     /translation="MITIVLLLSSCSKCLDCDYDYLLYQIENKLSYPIQVGFDCYSDP
                     GRNFETTIESNEIKDIIKGTTGLGFGLVSYDSAFMKVNTSEYIYQRTHETGLLSSSSY
                     KDNGTSKKNGRILYIRKLIVDEEFLLKSNE"
     gene            complement(1036..1566)
                     /locus_tag="PLJ82_02320"
     CDS             complement(1036..1566)
                     /locus_tag="PLJ82_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR40377.1"
                     /translation="MSRKYKFHNPEGVYFVSFAVQSWVDVFTRNVYKDILIENLIYCQ
                     NNKGLEIFAWCIMTNHVHLMIRAKEGYLLQDIVRDFKKFSCKAVIKAIKENYEESRKE
                     WLLKQLETKEGYSLWRGDNKPIELWSNAVITQKLHYIHQNPVEEGLVFKAEDYMYSSA
                     YDYTGENGMIDILVID"
     gene            complement(1637..2179)
                     /gene="idi"
                     /locus_tag="PLJ82_02325"
     CDS             complement(1637..2179)
                     /gene="idi"
                     /locus_tag="PLJ82_02325"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOR40378.1"
                     /translation="MCNKIAIVDLNDNIIGFEDKIKVHKEGILHRAFSVFIFNERNEL
                     LLQKRAKTKYHSPSLWSNTCCSHLSVGEEMKIAVKNRLYLEMGLKCNVKFIKKFSYKI
                     SFMDEMIENEIDYLYLGWSNNNPIINLDEVSDYKWISQDELIEELKKNPDKFTYWFLK
                     IIQLYDIFNLSINLTSKYNT"
     gene            complement(2186..3343)
                     /locus_tag="PLJ82_02330"
     CDS             complement(2186..3343)
                     /locus_tag="PLJ82_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR40379.1"
                     /translation="MKSLTRKMMLILALTIINQLISAQVKGVLIDKESKLPVVYANIY
                     TKVDEKIFGTVSNERGEFIFEYPSQTLFFSHINYEKLQVNSSSIKDTVFLTPTSHTLR
                     EVEAIVAYKQPQWIVDALERVLKERNKNYQTAEKIFSYHYQTETLNDSSGYAFYSEGN
                     IKFPMINDNQFQINAKNNIIKYKDNTAGVDFSNLQRMIYDDFILNYDKSFIKDNNFRP
                     IKSYKNQDNNIIQLSFTSKKYNDDNGYIIIDTLNNVILEIERNSGTDFNTKTHTSGFL
                     RSAATVSFGLSYNVWVTKTNSKYTKIGQSYYLAECKYKYLMKKTEKQKKTERTYFTSI
                     ESNIHFNDENNITGSGFIEIPKPYYLIAIRTKKMRLAEEAMSKVPAIHEKF"
     gene            complement(3291..4808)
                     /locus_tag="PLJ82_02335"
     CDS             complement(3291..4808)
                     /locus_tag="PLJ82_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40380.1"
                     /translation="MKIIQTFWTKNFIINNNYKGGWLSDEANFMCWALSCLNAKKIYG
                     NIELYTDTVGKKILIDKLNLPYDRVHLVFDNNDFMDSLPNELWAISKIYTYSLQNEPF
                     IHVDGDFILWENLDFQKDITCQNLELNFDLYKDIYNALLKKMNQLSECEFIRCLKDDF
                     LYKATNMGVFGGYNISHITQYANNVINFVKSNINNIDLILLDSIDVNCFLEQYYLYYF
                     CEKENISINTIHPEMYDDQFLNKTAFYFFPSDKMSFNHFLGNNKKNELINDYVKRKLN
                     ELYPAYYNKAQKNISNTFEYYSFCKNNVNLNVANIKKVFINNLHKCNAKIQDDLLVDF
                     ESFWEFKYNLLNQNYSFVKKRQNTFDISNLCDESLIRLNDNFITINKYLFPWEDVFLS
                     NKFTNEINEFNHISEKKKMYVYCIFFYTPFEGSIGTLWTTNLFAFILYKIINSDFQTI
                     SDLVTKLQSSLSKNSSESDNSYNKVKSFLLTFLEKINYYEIIDTKNDVNFSINNY"
     gene            complement(4876..5001)
                     /locus_tag="PLJ82_02340"
     CDS             complement(4876..5001)
                     /locus_tag="PLJ82_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40381.1"
                     /translation="MNIKELDELVNSFDLQELEERIELACFPGTSGTNTNVWWTF"
     gene            complement(5019..6125)
                     /locus_tag="PLJ82_02345"
     CDS             complement(5019..6125)
                     /locus_tag="PLJ82_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40382.1"
                     /translation="MEDKESLITFTNYDNKSFILHTEEGRHFIISREKKNEIENILQG
                     DDILLKKNLIDKINNIESNQKNNLMFLSDFQFKKTIITANTMKKISRFFVPLFNKNMM
                     IIISIIIICVLTYFFFINVPNVISANIPLSFSWILIFFVFFFHEIGHATACIKYKANP
                     GEIGFGITSFLPVMYANVTDAWRLERKERMIVNVGGIYFQNIFSLILFVTGITTKHYE
                     LYFISKTIFISTIYQFFPFYKSDGYWILSDIIAVPNLFKTSRDIFYKKFTNPRIKLSK
                     KKYLIMFYYLLISGIITYFVIKMGIHYLNYIVNLPLFLINSIGKIINNQFHQLNLDAN
                     YLWAVLYIIFTTKMIINNIKIFTLSKGADKVNIN"
     gene            complement(6133..8091)
                     /locus_tag="PLJ82_02350"
     CDS             complement(6133..8091)
                     /locus_tag="PLJ82_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOR40383.1"
                     /translation="MDYKENENLSISNVNKLYNEFSLLLEELHVSDGIKVRLLNDAFS
                     KYNFYVTLPFEIAKLYPESVVNENLLIKLSFYSYLYFSSILCFDKYYDNQIDILKNKN
                     LIRTFVFFVKEQALTGLLSIINQESNFWNEFNKLKIIFFESSNNENHIEIEEFSDDKY
                     FEIARNKSILSYAFVYSLKYLFGELVEEKSLLYGLDNFHMGFQIYDDYRDLKEDKINN
                     QMNYFNYKINKKSKKLKTSNDSFEYLLKLSYLDNTITDCLELALKFLRISLNVFADIK
                     QHSLINVTNETIYLIESELQFIKIAIKKANEKSTKSNSLIVKNDINKAINLSLYYLQN
                     NLNSNSFWEDFLTNAGYGKEWVTGYVLSMIGEINANLDFLSKPLEALLTSKSGYNNLI
                     VQDGDSSNFFIKTSNIFNKPILESKLNSWFAFSKSDGGWATYYSNDIKISMNFPLDAD
                     FKGWFTPQLCVSTVSAWVAKDFKDKKISDIYTRTIEYIISNQNKDGSWKSYWWTENIY
                     ATAFAILALPKDEKYNLYLKKAVIYLINKQNSNGSWSNGADECAFYTALSLKSLMDYF
                     LYSKSQIINDRIDIGINWLINNQMKDGSWKVSRILQIPFPDILDPLEVKDWRHTSFGL
                     NCLVDDHNRIFTTSTVYNCLCLYEKNFK"
     gene            complement(8194..8649)
                     /locus_tag="PLJ82_02355"
     CDS             complement(8194..8649)
                     /locus_tag="PLJ82_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOR40384.1"
                     /translation="MVPIQIVVPTQMLWASRQKDIAPINVESLKLNQIMIRKKIILDS
                     ETKIDLIDIEKITYIVLNSTNTEIHLITGDKYFSSKTLAFFESSVPDNVLFKINRNTI
                     VNLSYIASINKLTRKITIESDVSLCVSARQLKILIKRLKPILIRSSTNS"
     gene            complement(9176..10000)
                     /locus_tag="PLJ82_02360"
     CDS             complement(9176..10000)
                     /locus_tag="PLJ82_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOR40385.1"
                     /translation="MKILVVGANSYVGAAIYTQLREKYEVVGTYNAYPLFEELIQLDI
                     THAEEVEQIIQLHAPDTIVHVASNSNGNWCDQHPELAYAINVQATQTLVELANTYGAK
                     LVYISSLAATQGNNVYGKTKAAAEHYVQQTKAGFLILRPSLLVGFSPNTTNDRPFNRL
                     LKNITHHTPAVYDTSWQFQPTWLRHLCEVIEQCISQIVFNHIIPITVPQLCTRYSLAK
                     DILSDFGVTVLAEDKKDTTPLFVDTQDTLHALGLPVYSYQQMIDGIKEAISNFLRQ"
     gene            complement(10101..10799)
                     /locus_tag="PLJ82_02365"
     CDS             complement(10101..10799)
                     /locus_tag="PLJ82_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR40386.1"
                     /translation="MGNTLIRYKGVEICQKEQVVLKDVNLEIGEGEFYYLIGKVGTGK
                     SSLMKTMYAELPIETGEASLLDYDLLTITKSEIPFLRRQLGIIFQDFQLLIDRTVNEN
                     LLFVLKATGWKDKEEMQARIDAVLKEVGMSNKGYKMPHELSGGEQQRIVIARALLNKP
                     KLILADEPTGNLDPLTGAELVELLYTICKEENTTIIMATHNLGWIDAFPGKVLAISQN
                     KLCSGTKDECIFDY"
     gene            complement(10809..11402)
                     /gene="hisIE"
                     /locus_tag="PLJ82_02370"
     CDS             complement(10809..11402)
                     /gene="hisIE"
                     /locus_tag="PLJ82_02370"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOR40387.1"
                     /translation="MSLDFNKQGGLIPAIIQDSITNKVLMLGYMNEESLAKTKELGKV
                     TFYSRSKNRLWTKGEESGNFLHIVSIAEDCDNDTLLIKVNPVGPVCHTGADTCFFENN
                     VEDIMFLKFLQDFIDKRKQEMPEGSYTTSLFKKGINRMAQKVGEEAVETVIEATNGTD
                     EGFLYEASDLIYHLIVLLSSKGYRIEDLANELKKRHK"
     gene            complement(11419..12171)
                     /gene="hisF"
                     /locus_tag="PLJ82_02375"
     CDS             complement(11419..12171)
                     /gene="hisF"
                     /locus_tag="PLJ82_02375"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOR40388.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFVNFRDAGDPVELGASYSKQGA
                     DELVYLDITASHEGRKTFVDMVKRVAANVSIPFTVGGGINELTDVEKLLNAGADKVSI
                     NSSAIRNPDLINQIAQHFGSQVCVVAIDANYEENDWICYLNGGRIRTDKRLFSWAKEA
                     QERGAGEILFTSMTHDGVKTGYANDALAQLSDMLKIPIIASGGAGSKNHFKDAFLQGK
                     ADAALAASVFHFGEINISELKDYLRKHDICVR"
     gene            complement(12187..12909)
                     /gene="hisA"
                     /locus_tag="PLJ82_02380"
     CDS             complement(12187..12909)
                     /gene="hisA"
                     /locus_tag="PLJ82_02380"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOR40389.1"
                     /translation="MIQLIPAIDIIDGKCVRLSQGDYAKKTVYNENPLEVAKMFQDSG
                     ITRLHLVDLDGAKAQHIVNHKVLYTIATHTNLVIDFGGGLKTDEDLNIAFENGASMIT
                     GGSIAVKNPTVFESWIQKFGNEKIILGADVHDKKIAVSGWVESTEHDLFPFLESYTTK
                     GINKVICTDIAKDGMLQGTSVDLYKEINMQFPELFLIASGGVSAMSDIFALDEAGIPA
                     VIFGKAIYEGKISLQEIARYNQ"
     gene            complement(12917..13510)
                     /gene="hisH"
                     /locus_tag="PLJ82_02385"
     CDS             complement(12917..13510)
                     /gene="hisH"
                     /locus_tag="PLJ82_02385"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOR40390.1"
                     /translation="MQKVVVIKYNAGNIYSVVHALRRLGVEPVVTADEAVIRASDKVV
                     FPGVGEATTTMRFLKERKMDELITSLTQPVLGICLGMQLMCKSSEEGNVDCLGIFDVP
                     VKLFVPTQQTQKIPHMGWNQLYDVKSNLFSEQLNEQFVYFVHSFYVPMCEHTIATTDY
                     IQPFSAALNKNNFYATQFHPEKSGDVGEQILKNFLAL"
     gene            complement(13512..14384)
                     /gene="purU"
                     /locus_tag="PLJ82_02390"
     CDS             complement(13512..14384)
                     /gene="purU"
                     /locus_tag="PLJ82_02390"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOR40391.1"
                     /translation="MPQSSTKDSAILLLHCPDKQGILAAVTEFININKGNIIYLDQHV
                     DFVEKEFFMRVEWEIEGFLIPQEKISEYFYTLLGKKYGMNFRLYFNANKPRMAIFVSK
                     MSHCLFDLLSRYAANDWDVEIPLIISNHPDMEHVASKFDIPYYYFPITKETKQEQEAA
                     EIALLKEHKIEFVVLARYMQVLTAEFIAQYPQNIINIHHSFLPAFVGAKPYHAAYERG
                     VKIIGATSHYVTSELDAGPIIEQEVAKISHKDTVEMLIHKGQDLEKIVLSRGVEKHLE
                     RKVLVYKNKTIVFS"
     gene            14544..15011
                     /locus_tag="PLJ82_02395"
     CDS             14544..15011
                     /locus_tag="PLJ82_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOR40392.1"
                     /translation="MEVIILSSLLLAGIVLLLVELFLLPGTSIAGIGSALSFGGGIYY
                     AFAVYGVPVGVAVIIISVVVAAFAIYKFMKSKTLDTISLHTTIEGKNNPLEGISVSVG
                     DTGVAVSRLAPMGKVRINGNTIEVKVVSDMIDTGEQVKVTEISTSGIVVERNT"
     gene            15041..16009
                     /gene="floA"
                     /locus_tag="PLJ82_02400"
     CDS             15041..16009
                     /gene="floA"
                     /locus_tag="PLJ82_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839405.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOR40393.1"
                     /translation="MIESGMLTFIMVAVGFILLLIFFYYVPFLLWVSAKVSGVNVSLL
                     QLFLMRIRNVPPPILVRALIEGHKAGLEIKLDALEAHYLAGGHVERVVHALVSASKAN
                     IDLSFQMATAIDLAGRDVFEAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRANI
                     KQLVGGAGEETILARVGEGIVSSIGSNESHKAVLENPDTISKLVLKKGLDAGTAFEIL
                     SIDIADIDIGKNIGAQLQMDQAQADKNIAQAKAEERRAMAVALEQEMIAKAQEARAKV
                     IEAEAEVPKAMAQAFISGNLGIMDYYKMKNIEADTSMRENIAKPKK"
     gene            16132..17004
                     /locus_tag="PLJ82_02405"
     CDS             16132..17004
                     /locus_tag="PLJ82_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOR40394.1"
                     /translation="MKKRYESSELIVNADKSIFHLHLHPEQIADKIILVGDLGRVDVI
                     SGYFDTIESEAQNREFKTVTGTYKGKQITVVATGIGTDNIDIVVNELDALANIDLDKR
                     EDKDAFRKLTFVRIGTCGGLQKNIPVDSFLVSEQSIGFDGVLNYYANRDSVCDLDFEQ
                     AFIQQTSYSSKLTAPYCIHSTPSLVERIAQNDMLRGVTISAPGFYGPQGRELRLPLAF
                     PDLNQRIEDFEYKGKKITNYEMESSAIAGLATLMGHEAMTVCVVIANRMLKQYSKDYH
                     PAVKKLVELVLERI"
     gene            17110..17376
                     /locus_tag="PLJ82_02410"
     CDS             17110..17376
                     /locus_tag="PLJ82_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOR40395.1"
                     /translation="MKTYKFCQSCGYPLKKDTQGGGSEKDGEISKKYCSMCYDNGKFI
                     TPAEIDTAKKMKTFCIQEMKNTGINGFLAWLATRSIPTLERWKT"
     gene            17561..17893
                     /locus_tag="PLJ82_02415"
     CDS             17561..17893
                     /locus_tag="PLJ82_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40396.1"
                     /translation="MLYKRTNGIGFVAAKKRFAMEIEDIITTMAIIYCGGDIGKYGEV
                     DFRRHIPKETFTMVNNGIYLFIFRTLLITNHTRRGVVGTAYYEPESYYLLFNFFYSKI
                     WMIQIERI"
BASE COUNT         5825 a         3068 c         2756 g         6270 t
ORIGIN      
        1 tatattcact ttatacacct attcgtattt gtttaaactg ctgataatat gcagtctata
       61 atttcctaat aaaagtatac cgtggcacct atccatttgt tatttttttc tatctactag
      121 atttttcttt gattaaatct gttactaaaa aactcttgtg taaaactact aaagtttttt
      181 taatttgcaa agggggaagg agaatttatt atagttgata gttgatagtt aaaagttaaa
      241 agtttttaga gggcacttgg gcagtaggca atggcgagct aagggtaaca gaggtgttta
      301 gtgtttagtg tagttcgtac ggtagcagtg gctatggcaa gcttgtttgg ttcgaatatg
      361 gaaagctgga gctttccttt taacttcgac atagcgagac gctatgccga acacgcaaat
      421 tgtcaaaatt cacactacgg cgaacggggg ggggggcagt ttttataaat gactaccaaa
      481 cgtgcattta cttgaaaaat ttacttactc gttagatttc aataaaaact cttcatcaac
      541 aattaatttg cgtatataaa gaattctccc attttttttt gatgttccgt tgtctttata
      601 cgatgaagag gaaagcaaac ctgtttcatg tgttctttga taaatatatt ctgaggtatt
      661 aactttcata aatgccgaat cgtaagatac aagcccaaaa cctaagcctg tcgtaccttt
      721 aattatgtct ttaatttcgt tgctttcaat agttgtttca aaatttcttc ctggatcact
      781 ataacaatca aaaccaactt gtattggata acttagttta ttttctattt gataaagcaa
      841 atagtcataa tcacaatcaa gacatttaga gcatgatgat aataacaaaa ctattgtaat
      901 caaaattgca ctagttaaac ttaaattctt tcccattttt acctccagtt tatataatta
      961 ctaaacaatt cttctaaata tccttctgtt ggattatccc acgctggcgc gggtctgcga
     1021 cccgtgccat tcaacctaat caataaccaa aatatctatc atcccatttt ctccagtgta
     1081 atcgtaagca ctactataca tgtagtcttc ggctttaaat actaatcctt cttccaccgg
     1141 attttgatga atatagtgta gcttttgagt aataactgca ttgctccaca attcaatcgg
     1201 cttattatct cctctccaca ggctgtatcc ttctttcgtt tcaagttgtt tcagcaacca
     1261 ctcttttcgg ctttcctcat aattttcttt tatagcttta atgacggctt tacagctaaa
     1321 tttcttgaaa tctcttacta tatcctgaag caaatatcct tcttttgctc ttatcatcag
     1381 atggacatga ttggtcatta tgcaccatgc aaatatctca agtcctttat tgttttgaca
     1441 ataaataaga ttttcaatta gaatatcttt gtatacgtta cgtgtaaata catctaccca
     1501 actttgtaca gcaaacgaga caaaatagac tccttcagga ttatgaaact tgtattttct
     1561 actcataagt tactttatta ttggctatat gtatagacat gttggcacgg gtcacagacc
     1621 cgcgccagcg gggggcttag gtattatact tagatgttaa atttatagat agattaaaaa
     1681 tatcataaag ttggataatt tttaaaaacc aataagtaaa tttatctgga ttttttttta
     1741 attcctctat caattcatct tgtgatatcc atttatagtc tgacacttca tctaaattta
     1801 tgattggatt attgtttgac caccctagat acaagtagtc tatttcattt tcaatcattt
     1861 catccataaa actaattttg tatgaaaact ttttgataaa ttttacgtta catttcaatc
     1921 ccatttctaa atataaacga ttttttactg ctattttcat ttcttcgccg acactcaaat
     1981 gactgcaaca tgtattagac cataaagatg gtgaatgata tttcgttttc gctctttttt
     2041 gtaaaagcaa ttcatttctt tcattgaata taaaaaccga aaatgcgcgg tgaagtattc
     2101 cttctttatg tacttttatt ttatcctcaa atccaataat attatcattc aaatcaacga
     2161 ttgcaatctt attacacata attccttaga atttttcatg aatagcaggt actttcgaca
     2221 tcgcttcttc tgcaagtctc attttttttg ttctaatagc tattaagtaa tatggctttg
     2281 gaatttcgat aaaacctgag cctgtgatat tattttcatc gttaaaatgt atgttagatt
     2341 caatagatgt aaaatatgtt ctttcagttt tcttctgttt ttctgttttt ttcatcagat
     2401 atttatattt acattcagct aaataatatg attgacctat tttcgtatat tttgaatttg
     2461 ttttagtcac ccaaacatta taggatagac caaatgaaac tgtcgcagca gaacgtaaaa
     2521 atccagaagt atgagttttt gtattgaaat cagtgcccga atttctttca atttctaata
     2581 tcacattatt aagagtatca ataattatat acccattatc atcattatat ttttttgagg
     2641 tgaatgaaag ttgtattata ttattatctt gatttttata agattttata ggacgaaaat
     2701 tattatcttt tatgaagctc ttatcataat taagaataaa atcatcatat atcattcgtt
     2761 gtaaattcga gaaatctacc cctgctgtgt tgtccttata ttttataatg ttatttttcg
     2821 catttatttg gaattgatta tcgttaatca tcggaaattt tatattacct tcactataaa
     2881 aagcatatcc actagaatca tttaaagttt cagtttgata gtggtaagaa aatatttttt
     2941 ctgctgtttg ataattttta tttctttcct ttagaactct ttccaaagca tctacaatcc
     3001 actgaggttg tttataagct acaatcgcct caacttctct taaagtgtgg cttgtaggag
     3061 ttaaaaatac agtgtcttta atagatgagc tattaacttg tagtttttca taattgatgt
     3121 gggaaaaaaa caatgtttga gacggatact caaatataaa ctcacctctt tcattactta
     3181 cagttccaaa tatcttttca tcaacttttg tgtaaatatt cgcataaact acaggtaatt
     3241 tagactcttt gtctattaat actcctttta cctgtgcaga aattaactga ttaataattg
     3301 ttaatgctaa aattaacatc atttttcgtg tcaatgattt cataataatt tattttttct
     3361 aagaatgtta ataaaaacga ctttactttg ttgtatgaat tatctgattc actcgaattt
     3421 tttgataatg aactttgcag tttagtaact aaatcagaaa ttgtctgaaa atcagagttg
     3481 ataatcttat aaagaataaa agcaaataag tttgtagtcc acaatgttcc aattgaacct
     3541 tcaaatggag tataaaaaaa tatgcaataa acatacattt tcttcttttc agaaatatga
     3601 ttaaactcat taatttcatt agtgaattta tttgataaaa aaacatcttc ccaagggaaa
     3661 aggtatttat taatcgtaat aaaattatca ttaagtctaa ttaatgattc gtcgcagaga
     3721 tttgagatat caaaagtatt ctgtcttttt tttacaaagc tataattttg atttaatagg
     3781 ttgtatttaa attcccaaaa actttcaaaa tccactagta agtcatcttg aattttagca
     3841 ttacatttgt ggaggttatt tataaaaacc tttttaatat ttgctacatt tagatttaca
     3901 ttgtttttac agaatgagta atattcaaaa gtattagata tgtttttttg tgctttattg
     3961 taataagcag gatataactc atttaatttc ctttttacat aatcattaat tagttcattt
     4021 ttcttattat tccccagaaa atgattgaac gacattttat cagatgggaa aaaatagaat
     4081 gctgtcttat ttagaaattg gtcatcgtac atttcaggat gaattgtgtt aatgctaata
     4141 ttctcttttt cacaaaaata atataaataa tattgttcga gaaaacaatt aacgtctatt
     4201 gaatctaaga gaatcaaatc aatattattt atattcgact taacaaagtt tatcacatta
     4261 tttgcatatt gagtaatgtg tgaaatatta tagcctccaa aaacacccat attggttgct
     4321 ttatataaaa aatcatcttt aagacatcta ataaattcac attcactaag ttgattcatt
     4381 ttttttaata aagcattgta aatgtcttta tataaatcaa aatttagttc taaattttga
     4441 caagtaatat ctttttgaaa atctaaattt tcccataaaa taaaatcacc atcaacatga
     4501 ataaaaggct cattttgtaa tgaataggtg tatatttttg atattgccca taattcatta
     4561 ggtaaagaat ccataaaatc attattgtca aaaacaagat gcactctgtc ataaggaaga
     4621 tttagcttat caatcaatat ttttttccca acagtatctg tataaagctc aatattacca
     4681 taaattttct tagcatttaa acaacttaaa gcccaacaca taaaatttgc ttcatctgaa
     4741 agccatcctc ctttataatt attgtttatt ataaagtttt ttgtccaaaa cgtttgtata
     4801 attttcataa attacatttt tataattgag cgatattcaa cttatctatg ataatgctga
     4861 atatcgcttt tttagttaaa acgtccacca aacgttggtg tttgttccac tagtaccagg
     4921 gaaacacgct aactcaattc tttcttcgag ttcttgtaaa tcaaaagaat ttactaactc
     4981 atctaattct ttaatgttca ttttttataa ttgcggggct aattaatgtt taccttgtct
     5041 gcccctttag acaaagtaaa tatcttgata ttatttataa tcatttttgt cgtaaaaatt
     5101 atatataaaa cagcccacag ataattggcg tctaaattta attgatgaaa ttgattattg
     5161 attattttac caatgctgtt tatcaaaaat aatggtaaat tcacaatgta atttaagtag
     5221 tgtattccca ttttaatgac aaagtaagta attatcccac ttattagtaa gtagtagaac
     5281 ataatcaaat attttttttt gcttaatttt attcgcggat ttgtaaattt tttgtagaaa
     5341 atatccctag aagtcttaaa caaattagga actgctatta tatccgataa aatccaatac
     5401 ccatcacttt tataaaaagg aaaaaactga tagattgtcg atataaatat ggttttgcta
     5461 ataaaatata gctcataatg tttagttgtt attcctgtta caaataaaat taacgaaaaa
     5521 atattttgaa aatatatacc tcctacattt acaatcattc tttctttacg ttccaaacgc
     5581 catgcatcag taacatttgc atacattaca ggcaaaaaag atgtaatacc aaaccctatc
     5641 tcaccaggat tagctttata ttttatacat gccgtagcat gacctatttc gtgaaaaaaa
     5701 aagacaaaaa agattaaaat ccacgaaaag cttaaaggta tattggcaga tatgacattc
     5761 gggacattaa taaagaaaaa atatgtcagt acacaaatta ttataataga aataattatc
     5821 atcatatttt tgttaaataa tggaacaaaa aaacgagata tttttttcat cgtatttgca
     5881 gtaatgatgg tttttttaaa ctgaaaatca gataaaaaca ttaaattatt tttctgatta
     5941 ctttctatat tattgatttt atctattaaa ttctttttca ataagatgtc atcaccttgt
     6001 aggatatttt caatttcatt ttttttttct ctgctaatga tgaaatgtct tccttcttca
     6061 gtatgtagaa taaaactttt attatcgtaa ttagtaaaag taattaatga ctctttatct
     6121 tccatattct aattatttaa agtttttttc atataagcat aagcaattgt aaacggtaga
     6181 ggttgtaaat atcctattgt gatcatcaac cagacaattt aatccaaatg aagtatgtct
     6241 ccaatcctta acttcgagcg gatctaatat gtcagggaaa ggaatttgca aaattcgact
     6301 tactttccaa cttccatctt tcatttgatt attaataagc caatttattc caatatcaat
     6361 tctatcattt atgatttgac tttttgaata tagaaaatag tccattaaag actttaacga
     6421 aagagctgta taaaaagcac attcatcagc tccattactc caagaaccgt ttgagttctg
     6481 tttgttaatc agatatatta cagctttttt caaatataga ttatatttct catccttagg
     6541 taaagctaat atggcaaaag cagtagcata gatattttct gtccaccaat atgatttcca
     6601 actaccatct ttattttgat tcgaaattat atattctatg gttctagtat aaatatctga
     6661 aatcttttta tctttaaaat cttttgcaac ccaagccgaa acggtagaaa cacataattg
     6721 aggagtgaac caacctttaa aatctgcgtc taatggaaaa ttcatactaa ttttaatatc
     6781 gttactgtaa tatgttgccc atcctccgtc ggattttgaa aaggcaaacc agctatttaa
     6841 tttactttcc aaaattggtt tgttaaagat attagatgtt tttatgaaaa aattagatga
     6901 atcaccatct tgtacgatca aattattata acctgatttt gaagttaaga gtgcttctaa
     6961 tggtttactt aaaaaatcta aatttgcatt tatttctcca atcatactta atacatatcc
     7021 agtaacccat tctttaccat aaccagcatt cgttagaaag tcctcccaaa aactatttga
     7081 atttagattg ttttgtaaat aatataatga tagatttatt gctttattga tatcgttttt
     7141 tacaattaaa ctattacttt tcgtggattt ttcattagct ttttttatag caattttaat
     7201 gaattgcaat tcactttcaa ttagatagat agtttcgttt gtaacattta ttaatgaatg
     7261 ttgcttaata tccgcaaata cattaagtga tattctaaga aatttcaatg ctaattctaa
     7321 acaatcggtt atggtattat ctaaataaga taattttaat aaatattcaa atgaatcatt
     7381 gcttgtcttt agtttttttg actttttatt aatcttataa ttgaaataat tcatctgatt
     7441 gttaatttta tcctctttta aatctctata gtcatcataa atttggaatc ccatatgaaa
     7501 attatctaat ccatacaata aagacttttc ctcaactaat tcaccaaaca aatattttaa
     7561 tgaataaaca aaggcataag agagaatact tttgtttcgt gcaatctcaa aatacttatc
     7621 atcgctaaac tcttctattt caatatggtt ttcgttattt gacgactcaa agaatattat
     7681 ttttagtttg ttaaattcat tccaaaaatt gctttcttga ttaattattg acaacaatcc
     7741 tgttaacgct tgttctttta caaaaaaaac aaatgttcta attaaatttt tattttttag
     7801 tatatcaatt tgattatcgt aatatttatc gaaacataaa atggaagaga aatacaaata
     7861 agaataaaat gataatttaa ttaataaatt ttcattaaca actgattctg gatataattt
     7921 tgctatttca aaaggtagcg taacataaaa attatattta ctaaatgcgt catttagtaa
     7981 acgaacttta ataccatctg aaacgtgtaa ctcttctaac aataatgaaa attcattata
     8041 aagtttattt acattcgaaa ttgataaatt ttcattttct ttataatcca taaaccatac
     8101 tatgagattt caataataat aataataact atctgcaaat atattaaatt aacaaatata
     8161 aaaatcatag tgtgcggaat acctttgtga gcgttaagaa ttcgttgatg agcgtatcaa
     8221 aattggcttt aagcgtttaa tcaaaatttt taattgacgg gctgagacac ataaagatac
     8281 atcactctca attgtgattt ttcgggttaa tttattgata ctcgctatgt aggaaagatt
     8341 tacgatagta tttctgttta tcttgaataa aacattatca ggaacggaac tttcaaaaaa
     8401 agctaacgtt ttacttgaaa aatacttgtc tccggtaatt aaatggattt cagtatttgt
     8461 tgaatttaat acgatatagg ttattttttc tatatcaatt aaatcaattt ttgtttcaga
     8521 atcaaggatt attttctttc gtatcataat ttggttaagt tttaatgatt cgacatttat
     8581 aggtgcaatg tctttttgtc ggcttgccca caacatctgt gtaggtacca cgatttgtat
     8641 aggtaccaca gcgtggtacc tatattcact ttatacacct attcgtattt gtttaaactg
     8701 ctgataatat gcagtctata atttcctaat aaaagtatac cgtggcacct atccatttgt
     8761 tatttttttc tatctactag atttttcttt gattaaatct gttactaaaa aactcttgtg
     8821 taaaactact aaagtttttt taatttgcaa aggggggaag gagaatttat tatagttgat
     8881 agttgatagt tgatagttga tagttaaaag ttgaaagttt ttagagggca cttgggctgt
     8941 aggcaatggc gagctaaggg taacagaggt gtttggtgtt tagcgtttag cgtagttcgt
     9001 acggtagcag tggctatggc aagcttgttt ggttcgaaaa tggaaagcta gagcttccct
     9061 tctaatatgt acagttggta atgccgatgc atacaaacaa ataggacaat gagactttgt
     9121 cgaaattggg ctatattgtt tgtgcaatcg gtataacttc gagcgagaga aggttttatt
     9181 gtcttaaaaa attgcttatc gcctctttta tcccatctat catctgttga taactatata
     9241 caggtaagcc caatgcatgc agcgtatctt gtgtatcaac aaacaaaggt gttgtatcct
     9301 ttttatcttc ggcaagtacc gtaactccaa aatcggacaa gatatccttg gctaaggagt
     9361 agcgtgtaca cagttgtggc acggtaatag gaatgatatg attaaatact atttgggaaa
     9421 tacactgttc tattacctcg cacaaatgcc ttaaccatgt gggctgaaac tgccacgaag
     9481 tatcgtaaac ggcgggtgta tggtgggtaa tattctttaa aaggcgatta aagggtcggt
     9541 cgttggtagt attcggacta aaccctacca gcaaggacgg acgaagaatg agaaaacctg
     9601 ccttggtttg ttgcacatag tgttctgctg ctgccttggt tttgccatac acattattac
     9661 cttgcgtagc tgctagcgag gatatgtaca caagtttcgc tccataagta ttggctagtt
     9721 caaccagtgt ttgtgttgct tgtacattaa tggcataggc tagttctgga tgctgatcgc
     9781 accaattgcc attggagtta gacgctacat gtacaatagt atcaggtgca tgcaattgta
     9841 ttatttgctc cacctcttcg gcatgcgtaa tatccagttg tatcaattct tcgaataaag
     9901 gatacgcatt atacgtgcct actacctcat acttttctct taattgggta taaatagctg
     9961 ctcctacata actattggct cctactacta aaattttcat ttctatgtat attacgcatt
    10021 cattttatta gacagataca acaactacaa acaaaaaaag ggagaaaaac tcccttcatt
    10081 gtattggtgt aaaacaacca ttaatagtca aaaatacatt catctttggt gccactacac
    10141 aatttatttt gagagatagc caacacctta ccagggaaag catctatcca acccaaatta
    10201 tgggtagcca taataatcgt agtgttctct tctttacaaa ttgtatacag caattccaca
    10261 agctctgcac ccgttagcgg atcgaggttt ccagtaggct catctgccaa aatcaatttg
    10321 ggtttgttga gcaatgcacg agcaattaca atacgttgct gttctcctcc tgataattcg
    10381 tggggcattt tatagccttt attgctcatg cctacctctt ttaatacagc atcaatacgt
    10441 gcttgcattt cttctttatc tttccaacca gtagctttaa ggacaaacag taaattttcg
    10501 tttaccgtac ggtcaatcaa caactgaaaa tcttggaaaa taatacctaa ctgacgacgt
    10561 aaaaatggaa tttctgattt tgttatcgtt aataaatcat agtccaacaa gcttgcttca
    10621 cccgtttcaa taggcaattc agcatacata gttttcatca aactactttt tcctgtacct
    10681 acttttccta ttaaataata gaactcccct tctcctattt caaggtttac atctttaagc
    10741 acaacttgtt ctttttgaca aatttctacg cctttgtagc gtatgagtgt atttcccata
    10801 gcaatttatt atttatgtct ttttttcaat tcgttggcta aatcttcaat acgatatcct
    10861 tttgatgaca ataacacaat taagtgatat attaaatccg atgcttcgta caaaaatcct
    10921 tcatcagtac cattggtagc ctcaatcact gtttcaacag cctcttcgcc tactttctga
    10981 gccatacggt ttattccttt tttaaacaaa gaagtggtat atgaaccctc tggcatttct
    11041 tgtttgcgct tatcaataaa atcttgtaaa aatttcaaga acataatatc ttctacatta
    11101 ttctcaaaaa aacaggtatc tgcacccgtg tggcatacag gtcctacagg atttactttt
    11161 atcaataggg tatcattatc acaatcttct gcaatagata caatatgcaa aaaattgcca
    11221 ctttcttctc cttttgtcca caggcggttt ttactacggc tataaaaagt aacctttccc
    11281 aattcctttg ttttagctaa tgattcttcg ttcatatagc ccaacatcaa cactttattg
    11341 gtaatactgt cttgtataat agcgggaatt aagccgcctt gtttgttaaa atctaaactc
    11401 attgtattaa tcgttttttt atcgaacaca aatatcgtgt tttcttaaat aatcttttag
    11461 ttctgaaatg ttaatttctc caaaatgaaa cacacttgct gctaatgcgg catccgcttt
    11521 cccttgtaag aatgcatctt tgaaatggtt tttactaccc gctcctcccg acgcaataat
    11581 aggaattttt aacatatccg acaattgagc caaggcatca ttcgcatagc ccgttttcac
    11641 cccatcgtgt gtcatactcg taaataaaat ttcgccagct cctcgttctt gtgcttcttt
    11701 agcccatgaa aataaacgtt tatccgtgcg tattcgtcca ccatttagat agcaaatcca
    11761 gtcattttcc tcataatttg catcaatcgc tacgacacat acttgcgagc caaaatgttg
    11821 agcaatttga ttgattaaat ccggattgcg aatagccgat gaatttatag aaaccttgtc
    11881 ggctccagca ttcaatagtt tttctacgtc agttaattca ttgataccac cacctactgt
    11941 aaacggaata ctcacattag ctgccaccct tttaaccata tccacaaagg tttttcttcc
    12001 ctcatgcgaa gcggtaatat ccaaatacac tagctcatca gctccttgtt tactataaga
    12061 tgcacctaat tctacaggat cgcctgcatc tctaaaattc acaaaattaa taccttttac
    12121 ggtttttcca tcttttacat ccaaacaagg tataatacgt tttgctaaca tctcaatttt
    12181 tgttctttat tgattatatc gtgcaatttc ttgcaacgaa atttttcctt cgtaaatggc
    12241 tttcccaaaa ataactgctg gaatgcccgc ctcatctaag gcaaaaatat ctgacatagc
    12301 acttactccg ccactagcaa ttaaaaataa ctccgggaat tgcatgttaa tctctttgta
    12361 taaatcaaca gacgtacctt gcaacatacc atctttggca atatcggtac aaattacttt
    12421 atttattcct ttcgtggtat atgactccaa aaaaggaaac aaatcgtgtt ccgtgctttc
    12481 tacccaacca ctaaccgcta tctttttgtc gtgcacatct gcacccaata taattttttc
    12541 gttaccaaat ttttgaatcc acgactcaaa cacggttgga tttttaaccg caatacttcc
    12601 accagttatc atcgaagcac cattttcaaa cgcaatgttt aaatcctcgt ctgtttttag
    12661 tccaccacca aaatctatta ccaaattggt atgcgtagca atggtataca acaccttatg
    12721 gttgacaata tgttgcgctt ttgctccatc taaatctacc aagtgcaaac gagtaatacc
    12781 actatcttga aacatttttg ctacttcgag tggattttcg ttgtacaccg ttttcttggc
    12841 ataatcacct tgggataaac gaacacattt accatcgata atatctattg ctggaatcaa
    12901 ttgaatcata tatatattac aaggctaaaa agtttttcaa tatttgctca cctacatcgc
    12961 cacttttttc ggggtgaaat tgagtagcat aaaaattatt tttattcaat gccgcactaa
    13021 aaggttgaat ataatcggtt gttgcaatag tatgttcgca cattggcaca taaaaactgt
    13081 gcacaaaata cacaaactgt tcattcagtt gctccgaaaa caaatttgat tttacgtcat
    13141 acaactggtt ccatcccatg tgtggaattt tttgtgtttg ttgtgtcgga acaaataatt
    13201 tgacaggcac atcaaaaata cccaaacaat cgacattacc ctcctccgaa cttttacaca
    13261 tcaactgcat gcccaagcaa atgcccaaaa caggttgcgt aagcgatgta atcagttcgt
    13321 ccatcttgcg ttctttcaaa aaacgcatcg tggtagtagc ttctcccaca ccagggaaaa
    13381 caaccttatc agaagctcgt attacagcct catcagcagt tacaacaggc tctacgccta
    13441 agcgtcgtaa agcatgcacc accgaataga tatttcctgc attgtatttt ataacgacta
    13501 ctttttgcat actaactaaa tacgattgtt ttattcttat acacaagcac tttgcgttcc
    13561 aaatgttttt caaccccacg cgacagtaca attttttcca aatcttgtcc tttatgaatg
    13621 agcatttcta ccgtatcttt gtgcgaaatt ttcgcaactt cttgttcaat aatcggacca
    13681 gcatccaact ctgaagttac ataatggctt gtagcaccaa taattttcac tccacgttcg
    13741 tatgctgcat gataaggttt tgctcctaca aatgcaggta agaacgagtg atgtatattg
    13801 ataatatttt gtggatattg ggcaataaac tcagccgtaa gcacttgcat ataacgtgcg
    13861 agcactacaa attcaatttt atgttctttt aatagagcaa tttctgccgc ttcttgttct
    13921 tgttttgttt ctttcgttat tgggaaataa taatacggta tatcaaattt tgacgccaca
    13981 tgctccatat caggatggtt acttatgata agaggtatct ccacatccca atcatttgcc
    14041 gcatagcgtg ataataaatc gaataagcaa tgcgacattt tggacacaaa aatagccata
    14101 cgtggtttat tcgcattaaa gtataaccga aaattcatgc catatttttt gccaagcaag
    14161 gtataaaaat actccgaaat tttttcttgc ggaatcaaaa agccctcaat ctcccactct
    14221 acacgcataa aaaactcttt ttccacaaaa tcaacgtgtt ggtcgaggta tataatattt
    14281 cctttgttta tattgataaa ctccgtaact gccgccaaaa ttccttgttt gtcgggacag
    14341 tgaagcaata aaattgcgga gtctttggta gatgattgtg gcataagcgg tacataaatt
    14401 ctaaattccc cacaaaatta gtgtattttt actgaattat ggcagattta aacgagatat
    14461 tacaaatatg caaaaaaaat acaacaaaaa aaggaagtaa ttataatttt gagtatattt
    14521 acaaactaaa atttagttag aatatggaag ttatcatttt aagtagttta ttgctcgcgg
    14581 gtattgttct cttgttagtt gaactatttt tattacctgg cacctctata gcaggcattg
    14641 gttctgcgtt aagttttgga ggaggtattt attacgcttt tgccgtttat ggcgtacctg
    14701 tgggtgttgc tgttatcatt attagtgtag tagttgcagc atttgccatt tataaattta
    14761 tgaaatccaa aacattggat acaatttcct tacatacaac gattgaggga aaaaataatc
    14821 cattagaagg catttctgta tcagtaggag atacgggagt ggctgtttcg cgactagcac
    14881 ctatgggcaa agtgcgaatt aacggcaata caatagaagt aaaagtggta tcggacatga
    14941 tagatacagg cgaacaagta aaagtaactg aaatttctac gtcgggcatt gttgtagaac
    15001 gtaacacata aaaaactatt aatttattaa ataaaaaact atgattgaaa gtggtatgtt
    15061 aacctttatt atggtagcag tagggtttat cctgttacta atcttttttt actacgttcc
    15121 gtttttgcta tgggtatcag ccaaggtatc gggcgtaaat gtatcgttgc ttcagttatt
    15181 tttgatgcgt attcgaaatg tgccacctcc catattggtt agagcactca tcgaaggtca
    15241 caaagctggg ttagagatta aattagatgc gttagaagct cactatttag caggtggaca
    15301 tgtggagcgt gttgtgcatg cattggtttc ggcgtctaag gcgaatatcg atttgtcatt
    15361 tcagatggca acggctattg accttgcagg tagagatgta tttgaagctg tacagatgtc
    15421 agttaatccc aaagtaattg acaccccacc agtagctgca gtagctaaag atggtattca
    15481 gttaattgct aaagcccgcg taacggtacg tgcaaatatc aaacaattag tgggtggagc
    15541 tggcgaagaa actattttag ctcgtgtagg agaaggaatt gtatcttcta tcggttctaa
    15601 cgaatcgcat aaagcagtat tagaaaatcc agatacgatt tcaaaactcg tattaaaaaa
    15661 gggattagat gcgggtacgg cgtttgagat tttatccatt gatatcgctg atattgacat
    15721 aggtaaaaac attggggctc aattgcaaat ggaccaagca caagcagata aaaacattgc
    15781 acaagccaaa gccgaagagc gtagagctat ggcagttgcc cttgagcaag aaatgatagc
    15841 caaagcacaa gaagcacgtg ccaaagtaat tgaagcagag gcagaagtgc caaaagctat
    15901 ggctcaagcg tttattagtg gcaacttggg tattatggat tattataaaa tgaaaaacat
    15961 cgaagctgat acttctatgc gcgaaaacat agctaaacca aagaaataaa cattcgtact
    16021 tatttctttt cttagagtta catactcact tgtttttggc actagaagta aatcagtgat
    16081 cgaaaaataa gtgagtattt atttactatc taatcaataa aagcaccaac tatgaaaaaa
    16141 agatatgaaa gttcagaatt aattgtaaat gcagacaaaa gcatcttcca cttacatttg
    16201 catccagaac aaattgctga caaaataatt ctcgtaggag atttgggacg tgtagatgtg
    16261 ataagtggct attttgacac catagaaagc gaagcacaaa accgtgaatt taaaaccgtt
    16321 accggcacct acaagggaaa acaaataacg gtagtagcca caggaattgg taccgacaac
    16381 attgatatag tagtaaatga attagatgct ttggctaata tcgatttaga taaaagagaa
    16441 gacaaagatg cttttcgcaa acttacattt gttcgtattg gtacttgcgg cggtctgcaa
    16501 aaaaacatac ctgtagatag ttttttagtg tctgaacaat ctattggctt tgatggagtc
    16561 cttaattatt atgcaaaccg agatagtgta tgcgacttag attttgaaca agcatttatt
    16621 cagcaaacga gctattcgag taagctaact gcaccctact gcattcattc tacgccaagt
    16681 ttggtagagc gtattgcaca aaacgacatg ctacgtgggg taactatttc tgctcctggt
    16741 ttttacggac cacaaggaag agaacttcgt ttaccattgg cttttcctga tttaaaccaa
    16801 cgcattgaag attttgagta taaaggcaaa aaaattacca attacgaaat ggaaagttct
    16861 gctattgcgg gcttagccac attgatggga cacgaagcca tgactgtgtg tgtagtaatt
    16921 gccaacagaa tgctcaaaca atacagtaaa gattatcatc ctgctgtaaa aaaattggta
    16981 gaattggtgt tggaacggat ataggatttc ataaactgtg tgctgttaag tacgtaataa
    17041 tgattgggtt catgtgtgta tttattctgt tatcgaagga agttagtata agtaattcaa
    17101 taaactgtga tgaaaacgta taaattttgc caaagttgtg gttatccgtt aaagaaagac
    17161 acccaaggtg gtggttcgga gaaagacgga gaaataagta agaaatattg ctccatgtgt
    17221 tatgataatg gaaaatttat aacccctgcc gagattgata ctgctaaaaa aatgaaaaca
    17281 ttttgcatac aagaaatgaa aaatactggc ataaatggtt ttcttgcatg gctagctact
    17341 cgtagcattc ctacattgga aagatggaaa acctgatgct cgtgcacata aacaattaca
    17401 gctcacatac ttttttttga cacacgtttg ctgagttgta gcctcgctga ttaggctacg
    17461 ccgaacaagg ggatgggaca tcgaagtttt ctttcgcttt attaaacagg aacttaactt
    17521 taaacatttt ctgtctgtca atgaaaatag tttgcaggta atgctttaca aacgtaccaa
    17581 tggaatcggt tttgtggctg caaaaaaaag atttgcaatg gaaattgaag atataattac
    17641 tacaatggca ataatctatt gcggtggaga tattggaaaa tatggagaag tcgattttag
    17701 aagacatatc ccaaaagaga catttacgat ggttaacaat ggcatatact tatttatctt
    17761 ccgaacactc ctgattacaa atcacacccg acgaggcgtc gtaggcacag cctactacga
    17821 accagagtct tactacttac tcttcaactt cttctatagc aaaatctgga tgattcagat
    17881 tgaaagaata taaataattt acttcactac ctgaatagt
//
LOCUS       DAOSRP010000021        17432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3391_length_17432_cov_5.597859, whole genome shotgun sequence.
ACCESSION   DAOSRP010000021 DAOSRP010000000
VERSION     DAOSRP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17432
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_17432_cov_5.597859"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLJ82_02420"
     CDS             complement(<1..163)
                     /locus_tag="PLJ82_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050377450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOR40397.1"
                     /translation="MNLLNFVAQYPDEASCRVKFKEYRDQQGVVCPHCGGTSHYWKHD
                     KESYECKKCG"
     gene            complement(294..608)
                     /gene="trxA"
                     /locus_tag="PLJ82_02425"
     CDS             complement(294..608)
                     /gene="trxA"
                     /locus_tag="PLJ82_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR40398.1"
                     /translation="MAKEFTESNFEELLQNNVVVVADFWAEWCGPCRMITPIVEELAS
                     AYEGKVLIGKVNVDEEGDLATKYGIRNIPTVLFFKNGQIVDKQVGAAGKNVFEDKINA
                     LL"
     gene            complement(682..4530)
                     /gene="dnaE"
                     /locus_tag="PLJ82_02430"
     CDS             complement(682..4530)
                     /gene="dnaE"
                     /locus_tag="PLJ82_02430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR40399.1"
                     /translation="MKSFVHLHVHSYYSLLDGMSSISALVDKAQKDGMPAIALTDHGS
                     MFGIKEFYDYTKKKNKKVKEDIAILEKELLAATDDKQRELWKEKIAATTCKYVKPIIG
                     SEVYVAKRTRFDKTEEIDRSGNHLVLLAKNKKGYRNLCKLVSYGYIEGYYYRPRIDKE
                     LLEKYSEGLIVSSACLGGEIHRLVEKGNIEEAEKSILWFKRVFGDDYYLELQRHETDK
                     PGADTHVFEKQQRQNKVLIELARKTNTKFIATNDVHFVEEEHAEAHDRLICLSTGKDL
                     DDEKRMRYSKQEWLKTTDEMATIFSDLPEAIENTLEIANKVENYSIDEPNPIMPTFDI
                     PIDFGTEEGYRQQYTHQDLLKEFTADEHGVMHLSEKEALEKVHRLGGIDRLYRIKLEA
                     DYLRKITFDGAQVRYGENIPQEVKERIEFELHVMKTMGFPGYFLIVYDFIQAARKMEV
                     TVGPGRGSAAGSVVAYCLRITDIDPLKYDLLFERFLNPDRISMPDIDIDFDDDGRSKV
                     LDYVAHKYGKERVAQIITYGTMATKMAIKDVARVQKLSLPESERLTKLIPTSFPEENG
                     KSPKVTISNCIERIPELREARYSSDARLSDTLKYAEMLEGTVRQVGIHACGVIIGADD
                     LTNFVPISIDKNNSETLVTQYEGSTIETVGLIKMDFLGLKTLSIINETLRNIKASKGI
                     TIDIDTIPIDDKKTYELYSKGLTTGTFQFESSGMQKYLRELKPNRFEDLIAMNALFRP
                     GPMAYIPSFINRKHGIEEIQYDIPIMEKRLKETYGITVYQEQVMLLSRDLAGFTRGES
                     DELRKAMGKKLQDKMDALKVKFINGATKNGYGPIELLEKIWKDWAEFAKYAFNKSHAT
                     CYSWISYQTAYLKANYTADFMAANLTRNKDNITEITKFMEECKQLGIKVLGPSVNESN
                     LNFISNPAGDIRFGLGGIKGVGYGAVEAIVTERQANGRYTSVFNFLERVNLNACNKRA
                     IESLALSGAFDEFDDVKREAFFAENSKGEVVLETLMRYASRYQLDQQSSQQSLFGDLE
                     SVAIQRPEIPQVQSWSNLYKLTKEKDMIGIYLSAHPLDFYEIELASLCTQDVTRLKEL
                     SELHDSIGSNPVKIAGIITKVNRLISQNNQPFARFTLEDYSGSHEISVFGKQFTELDN
                     MLRENYYVLIHGVVQERQADYKFKPKPEDEARGRELVFKIQTIELLETIKDKGIEELK
                     IQIPLLNISYELIAELSALLSENVDKKAKTRFCVEVIDPETRWIVKLNKKDGKITVNK
                     PIFHFLKEKKENQILDFIIS"
     gene            complement(4668..5228)
                     /locus_tag="PLJ82_02435"
     CDS             complement(4668..5228)
                     /locus_tag="PLJ82_02435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOR40400.1"
                     /translation="MINVAIFASGSGSNAENIIRYFTGSQLVNFSCILSNKQDAFVHQ
                     RAKKLLIPSYTFTKQQFEDGTVLSFLMDEKINFIILAGFLLKVSDNIITQFPNKIVNI
                     HPALLPKFGGKGMYGDYVHQAVVDAHETQTGITIHFVNNKYDDGEIIFQATCDVLSTD
                     TPDDVAQKVHALEYEHFPHVLKMLWS"
     gene            5530..5763
                     /locus_tag="PLJ82_02440"
     CDS             5530..5763
                     /locus_tag="PLJ82_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016665433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR40401.1"
                     /translation="MSEVAEKVKAIIVEKLGVEASEVVPAASFTNDLGADSLDTVEMI
                     MEFEKEFGISIPDDQAQKIATVGDAISYIEANK"
     gene            5782..7041
                     /gene="fabF"
                     /locus_tag="PLJ82_02445"
     CDS             5782..7041
                     /gene="fabF"
                     /locus_tag="PLJ82_02445"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOR40402.1"
                     /translation="MELKRVVVTGLGAVTPIGNDVPTFWNSLIQGVSGAKPITRFDAS
                     KFKTQFACEVKDFDPNQYFDRKEARKYDLFSMFGIVAAKEAVEDAGIDVEKVDLDQIG
                     VIFGTGIGGIKTFAEEVAGFVQNESIPRFNPFFIPKMIIDILPGHVSIMYGFRGINFS
                     TSSACASSTHALADAFNYIRLGKANIMVAGGSEAAITDVGVGGFNALHAISTRNDSPE
                     TASRPFSLSRDGFVMGEGAGCLIFEELEHALARGAKIYAEVKGAGMTADAHHMTAPHP
                     EGLGAINVMKSALKDADMQPDEIDYINVHGTSTPLGDIAETKAIKDVFGEHAYNLNIS
                     SSKSMTGHLLGATGAVEAIACVLSVQNDIVPPTINFTEGDEDPEIDYKLNFTFNKAQK
                     RPVNAVLSNTFGFGGHNGSIIFKKFAK"
     gene            7066..7881
                     /gene="rnc"
                     /locus_tag="PLJ82_02450"
     CDS             7066..7881
                     /gene="rnc"
                     /locus_tag="PLJ82_02450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOR40403.1"
                     /translation="MIKQIINRIRFISFTKNEPYFVLRNLLGFSPKENSYYQLALLHR
                     STGKRNINGVIIDNERLEFLGDAILSAIISDYLYQKFPLKKEGFLTNLRSKIVQREYL
                     DYLAVQMGLDKLMFSQNKHIVSKNAVHVYGNALEALIGAIYLDQGYKKCQQFLEKQVI
                     EKHMNVNEIVKKEYNFKSKLIEWAQKRKYSIIFVVTNSEVNKEQNITTFYTSVLIENI
                     EVGTGCGLNKKESQQKAARDALHKIKKKATGILINTKYKERIELENHSELKKL"
     gene            7901..9721
                     /locus_tag="PLJ82_02455"
     CDS             7901..9721
                     /locus_tag="PLJ82_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOR40404.1"
                     /translation="MKASNHPIHLAHLVFEKAKQYGERAALHYRNDSTKTWVPMSWNE
                     IAKQVTFISKGLVSIGLEENQNVGIFSQNMCEIIIADFAIMGNHAVSVPLYATSTAPQ
                     IDFIVKDAEIGLLFVGEQYQYDVAMEVKKHSKFLKKIIAIDPNIDLKNDPENMYFFDL
                     LLLGQTVNNDDIVEKRKSKASENDLVNILYTSGTTGVPKGVMLHQYNYSVAIRIHKER
                     LAEVTDKDTSMCFLPITHIFERAWTYLCLERGVQVYVNKRPIEIQQTILEVQPTLMCT
                     VPRYWEKVYAAILEKMETLSPALKKIFTWAIQVGKVYNLEYYRHRKSAPILLRLKYIL
                     AEKLVFNKIKSAAGLTNLHFFPCAGSRLADDINEFIHSLGINLVYGYGLTESTATVSC
                     YYPENKEYIIGSIGKIMPEVDVKISDQGEILLKGSTITAGYYKRPDANAESFEDGWFK
                     TGDAGYIDAANNLYITDRIKDLFKTAGGKYIAPQMLESRLSELKFVDQVAIIGDQRKF
                     VTALIVPTFDTLKIYASNHNIPYQSIEELISHPDIIKFYSDCIKEQMKDLASYEQIKR
                     FTLLPKAFTMEDGLLTNTLKLKRKEVAKVFENIIEKMYID"
     gene            9798..10905
                     /gene="prfB"
                     /locus_tag="PLJ82_02460"
     CDS             join(9798..9860,9862..10905)
                     /gene="prfB"
                     /locus_tag="PLJ82_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOR40405.1"
                     /translation="MITIEQLKETIERQQALRRYLDIDAKLVALQEEELRTQDPLFWN
                     DSKSAEIQMKKIKELKFWIETYNKVYQATEELKLAFDFYKEAIVAEQDVDEAYSVAMD
                     CISNLEMKNMLSAEEDNMGAIIKIVSGAGGTESQDWASMLLRMYQRWCERNTYKCELT
                     NFQAGDEAGIKTATLQVEGELAYGYLKSENGVHRLVRVSPFNAQGKRMTSFASVFVTP
                     LIDDSIEIDINPADITWETFRSGGAGGQNVNKVETGVRLRYWYKDTPTSEPIEIIIEN
                     TESRSQFGNKDNAMRLLKSQLYEKELEKRRAASAQIEAGKKKIEWGSQIRSYVFDDRR
                     VKDHRSNYQTSDVQGVMDGDIDAFIKAYLMEFSN"
     gene            10925..12652
                     /gene="lysS"
                     /locus_tag="PLJ82_02465"
     CDS             10925..12652
                     /gene="lysS"
                     /locus_tag="PLJ82_02465"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOR40406.1"
                     /translation="MSGIELSEQEIFRRQSLEQMRSMGIEPYPAEEFPTNAFSTEIIS
                     NFNENEKRDVAIAGRIMSRRIMGKASFIELQDSKGRIQVYISRDEICPEEDKELYNTV
                     FKKLLDIGDFIGIKGYVFRTQMGEISVHASELKVLAKSLRPLPIVKIKDGIAYDAFTD
                     AESRYRQRYVDLVVNEQVKEIFLKRTNVFNSMREFINQHGYIEVDTPVLQPIPGGAAA
                     RPFTTHHNALDIPLYLRIANELYLKRLIVGGFEGVYEFSRNFRNEGMDRTHNPEFTVM
                     ELYVSYKDYNWMMDFTEKMLEKICIDVNGTTEVPMGEKIINFKAPFKRISMTDAIKEH
                     TGFDITGKNEKELREICNALHIETDETMGIGKLIDEIFGEKCEGNYIQPTFITDYPIE
                     MSPLTKKHRSKAGLTERFELMVNGKELANAYSELNDPIDQRERFEEQLRLSEKGDDEA
                     MFIDQDFLRAMEYGMPPMSGIGIGMDRLVMAMTNQTSIQEVLLFPQMRPEKIAKKDTP
                     ARFIELGMSEEWAQAIVKHSSLSIKDIASNIDAKLHQQLCGINKKHKLSLPNPTLADV
                     QVWIKKASE"
     gene            12666..13661
                     /locus_tag="PLJ82_02470"
     CDS             12666..13661
                     /locus_tag="PLJ82_02470"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOR40407.1"
                     /translation="MKAKRICIMGGGSWATALAKITMSKGDKINWYMRRPEQIEIFQR
                     LGNNPSYLSSIRFDTKKIKFYADINLAVKNSDILVFATPSPFLKQHLQKLTRTLSNKI
                     IVSAIKGIVPDENMIINDYFQKFYGVKQENIAIIAGPCHAEEVALERLSYLTIACANN
                     ELGTYLAERLSCSFLKTSITSDINGIEYSSVLKNVYAIAVGICHGLKYGDNFQAVLIS
                     NSIREINRFVDAIERNNERNTQDSAYLGDLIVTAHSRFSRNRTFGTMIGKGYSVKTSQ
                     LEMEMIAEGYYGTKCIKEINDTLNVDMPILDAVYNILYERIAPTIEIKLLTEKIR"
     gene            13664..15007
                     /locus_tag="PLJ82_02475"
     CDS             13664..15007
                     /locus_tag="PLJ82_02475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOR40408.1"
                     /translation="MENIKLVIDNTLGFISKDKVFAYEKEVSACNSMLHNGTGKGNDF
                     LGWLSLPSSTSAELINDIKQTADLLRNECEVVVVAGIGGSFLGAKAVIDALSNTFIWL
                     KKDTNTPVIVYAGNNIGEDYLAELMDYLSDKKFGIINISKSGTTTETALAFRLLKTQL
                     EKQVGKAKAKNLIVAVTDASKGALRTLATQEGYKTYVIPDNVGGRFSVLYPVGLLPIA
                     VAGLDLNQLLAGARKMEEVCGLNTPFAENPAAIYAATRNELYRNGKKIEILANYQPKL
                     HFFAEWWKQLFGESEGKENKGIFNAAVDFSTDLHSMGQWIQEGERTIFETVISIKQAS
                     KKVVVPTDNDNLDGLNFLAGKRVDEVNKMAELGTMLAHVDGGVPNMKIEVPELNEYYI
                     GQLIYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLEKPGFEAETKAIKARL"
     gene            15126..15200
                     /locus_tag="PLJ82_02480"
     tRNA            15126..15200
                     /locus_tag="PLJ82_02480"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15161..15163,aa:Val,seq:tac)
     gene            15232..15306
                     /locus_tag="PLJ82_02485"
     tRNA            15232..15306
                     /locus_tag="PLJ82_02485"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15267..15269,aa:Val,seq:tac)
     gene            15390..16547
                     /locus_tag="PLJ82_02490"
     CDS             15390..16547
                     /locus_tag="PLJ82_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40409.1"
                     /translation="MLKRLKTHLTFLCSFILVAHVYSAQYQGIILDSQSHKAIPYVSI
                     HTSNNNDIYGSMSNDLGEFSISFDFDSLVFQHINYTPYILKKQSYKDTLYLNPLSYTL
                     NEIDVYATESLWIRKKLHLVVKNIKKFYTLPSSIQQDYEYNTYTLSDSSGYAFESKGI
                     LQSNKNEYSILPILSAIKYKDSTAGTDFSNLRRMLHDDFLPRFTSNFIKNNSFRENKG
                     FRHTNPNVVQLFYFSNDNLSDDGYLVIDTLKNVILEAQQNLGTQSNIENNTSSLLRKA
                     AELIGFTYNDWITQTQYIYTEINGSYLLQSCIYKLYMRTTTENKKLSNSYFVSTESTV
                     RLSNTTLTNSVSNSKQFLILPKPYYIVPIYTKTMREEEEKLQRIKRTYKKF"
     gene            complement(16560..17039)
                     /gene="msrA"
                     /locus_tag="PLJ82_02495"
     CDS             complement(16560..17039)
                     /gene="msrA"
                     /locus_tag="PLJ82_02495"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOR40410.1"
                     /translation="MIEKAYFASGCFWGTEYYFMQLTRIATQVGYMGGLTFAPTYREV
                     CTGDTGHIETVEIVYDNEQLQYETLVKLFFETHNFSQINGQGPDIGSQYVSVIFYTNE
                     KQRDIATYYITLLQTKGYFVATQLRQAEEFWVAEDYHQTYYTKTGGTPYCHKYTKIF"
     gene            complement(17039..17392)
                     /locus_tag="PLJ82_02500"
     CDS             complement(17039..17392)
                     /locus_tag="PLJ82_02500"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HOR40411.1"
                     /translation="MNKLTKEEEHIIVHKGTELPFSGEYVHTKEDGIYSCRRCDTPLF
                     LSDDKFDSECGWPSFDDAIKEAVLEIPDMDGRRTEIVCANCHGHLGHVFEGEGFTEKN
                     VRHCVNSLSIKLMKK"
BASE COUNT         6063 a         2923 c         2851 g         5595 t
ORIGIN      
        1 taccgcattt cttgcattca taactctctt tgtcatgttt ccaataatga cttgtaccac
       61 cgcaatgtgg acaaacaaca ccttgctggt ctcgatactc tttaaattta actctacaac
      121 ttgcttcgtc tgggtattgc gcaacaaaat ttaataaatt catgcctttt ctgattagtt
      181 acttcaaaaa taatcatttt aggcgaaata tcggataatc attttttttt aaatagaaaa
      241 agggagttca tactaacatg aaactccctt tttttttcga aaagatatag gtattacaat
      301 agtgcgttga ttttatcttc aaatacattt ttacctgccg cacctacttg cttatcaaca
      361 atttgaccat ttttaaaaaa caatacagta ggaatatttc ggataccata tttggtagct
      421 aagtctcctt cttcatcaac attaactttc ccaatcaata cttttccttc gtaagcacta
      481 gcgagttctt caacgatagg ggtaatcata cgacagggtc cacaccattc tgcccaaaaa
      541 tctgctacta cgactacgtt attttgtaat aactcttcaa aatttgattc tgtaaattct
      601 tttgccatgt ttgtctattt ttattattag ttgatataaa tatatatgtg caaatgtata
      661 taatttcaat gtaaaatcct attaagaaat gataaaatca agaatttgat tttctttctt
      721 ttctttaaga aaatgaaaga ttggtttatt gactgttatt ttcccatctt ttttattcaa
      781 tttaacaatc caacgtgttt cgggatctat aacttcaaca cagaaacgag tttttgcttt
      841 tttgtctaca ttttctgaca ataaagcaga taattcggca attaattcat acgaaatatt
      901 taaaagggga atctgtattt ttaattcttc tataccttta tctttaattg tttccaataa
      961 ttcaattgtt tgaattttaa acaccaattc acgtccacgt gcttcatctt ctggtttagg
     1021 tttaaactta tagtctgctt gtcgttcttg taccactcca tgtatcaata cataataatt
     1081 ttctctcaac atattgtcta gctcggtaaa ttgtttgcca aatacactaa tttcatgcga
     1141 tcctgaataa tcttcgagtg taaagcgagc aaagggttga ttattttgag aaattaatcg
     1201 gttaactttg gttataatac ctgctatttt aacaggattt gaacctatac tatcatgcaa
     1261 ttctgataat tcttttaatc gtgtaacatc ttgtgtacac aaggatgcaa gttcaatttc
     1321 gtaaaaatct agaggatgag ccgacaagta aatcccaatc atgtcttttt ccttagttag
     1381 tttatataaa tttgaccacg attgtacttg aggaatttca ggtcgttgaa tagcaacaga
     1441 ctctaaatct ccaaacaacg attgttgaga agattgctga tcaagttggt atctactagc
     1501 ataacgcata agggtttcta aaacaacctc tcctttagaa ttttctgcaa agaaagcttc
     1561 tctttttaca tcatcaaatt catcaaatgc tcctgataaa gccaaacttt ctatcgctct
     1621 tttgttacaa gcatttagat tcaccctttc taaaaaatta aaaacacttg tgtaccttcc
     1681 atttgcttgc ctttcagtta caatagcttc tacagcacca taccccactc cttttatgcc
     1741 accaagtcca aaacgaatat cgccagctgg attagatatg aagtttaaat tactttcgtt
     1801 tacacttgga cctaaaactt taataccaag ctgcttacac tcttccataa acttggtaat
     1861 ttcagtaata ttatccttgt tcctcgtaag gttcgccgcc ataaaatcag ccgtatagtt
     1921 agcctttaaa taagcagtct gataagatat ccacgaataa caagtagcat gtgatttatt
     1981 aaaagcatac tttgcaaatt cagcccaatc tttccaaatt ttttccaaca attctattgg
     2041 accatagcca tttttggttg caccattgat aaatttaact tttaaggcat ccattttatc
     2101 ctgcaacttt ttccccatag cttttcgcaa ttcatcagac tcaccacgtg taaatccagc
     2161 taaatctcga ctaagtaaca tcacctgttc ttgatacaca gtaatcccat acgtttcttt
     2221 taatcgtttt tccatgatgg gtatatcata ctgtatttcc tctattccat gttttctgtt
     2281 aataaagctt ggaatgtaag ccatcggacc tggtcgaaac aaagcattca tggctattaa
     2341 atcctcaaaa cggttgggtt ttagttcacg caaatatttt tgcattccgc tcgattcaaa
     2401 ctgaaatgta ccggtggtca atcctttgct atacaactca taggtttttt tatcatctat
     2461 gggtatagta tcaatatcta tagtgatacc ttttgatgct ttgatattac gcaacgtttc
     2521 gttaataata gacaaggttt tcaaccctaa aaagtccatt tttattaaac caaccgtttc
     2581 aatagttgat ccttcatatt gtgtaactaa agtttcggaa ttatttttat caatactaat
     2641 aggaacaaaa ttggttaaat catcagcacc aataataaca ccacatgcat gtatgcctac
     2701 ttgtcttact gttccttcaa gcatttcggc atattttaaa gtatccgata agcgagcatc
     2761 tgaagaataa cgtgcttcac gtaattcagg aattcgttct atacaattag agatagttac
     2821 tttaggcgat ttgccatttt cttctggaaa actagttgga atcaacttag ttaatctttc
     2881 tgattcggga agagataatt tttgcacgcg tgccacatct ttgatagcca ttttagtcgc
     2941 catcgttcca taagtaataa tctgagccac acgctctttg ccatatttat gagcgacata
     3001 atcaagcact tttgatctgc catcatcatc aaaatcaata tcaatatcag gcatcgagat
     3061 acgatcagga tttagaaaac gttcaaacag caaatcatat tttaaaggat ctatatctgt
     3121 aattcgcaaa caatatgcca ctacggaacc tgcagccgaa ccccttcctg gacctactgt
     3181 aacttccatc ttacgagcag cttggataaa gtcgtacaca attagaaaat atcctggaaa
     3241 acccattgtt ttcattacat gaagttcgaa ttcgatacgt tctttcactt cttgtggaat
     3301 attttctccg tatcgtactt gtgctccatc aaatgtaatt tttcgcaaat aatctgcttc
     3361 tagtttaatg cgatacaacc tatcaatacc tcccaatcta tgtacttttt ccaaagcctc
     3421 tttttcagaa agatgcataa caccatgttc atctgcagta aactctttca gcaaatcttg
     3481 atgtgtatat tgttggcgat acccctcttc tgttccaaaa tctattggaa tatcaaaagt
     3541 aggcattatt ggattaggtt cgtcaatgct ataattttca accttgtttg caatttcaag
     3601 cgtattttct atggcttcgg gtaaatctga aaatatagta gccatctcat ccgtagtctt
     3661 tagccactct tgtttactat accgcatacg cttttcatca tccaaatctt taccagtact
     3721 caagcatata agtctatcat gagcctctgc atgttcttct tctacaaaat gaacatcatt
     3781 ggttgcaata aatttcgtat tcgttttacg tgcaagttct attaatactt tattctgtct
     3841 ttgttgcttt tcaaatacat gtgtatctgc tcctggtttg tccgtttcat gtcgttgcaa
     3901 ttccaaataa taatcatcac caaaaacacg tttaaaccac aaaatacttt tttctgcttc
     3961 ctctatattc cctttttcta caagacggtg aatttcaccc cccaaacaag ccgaagacac
     4021 aatcaagcct tcgctgtatt tttctagcag ttctttgtca atgcgaggac gataataata
     4081 accctcgata tatccatacg aaactaattt acatagattt cgatagccct ttttattttt
     4141 tgctaacaat acaagatgat ttccacttct gtctatttct tctgttttat caaaccgagt
     4201 acgcttagct acatacactt cgcttcctat aattggtttg acatacttac aagtagttgc
     4261 tgcaattttt tctttccaca attctcgttg tttgtcgtct gttgcagcta gtaattcctt
     4321 ttctaatata gcaatatctt cctttacttt tttatttttc tttttggtat aatcataaaa
     4381 ctcttttatg ccaaacattg aaccgtggtc tgtaagagca atagcaggca tgccatcttt
     4441 ttgagcctta tcaaccaagg cactaataga agacatacca tccaataaag aataataaga
     4501 gtgaacgtgt aaatgtacaa aggatttcat atataaaaag gtaaattatt tcagaaatcc
     4561 aaagttagtg attctcttaa agtagaaaaa ataaaattct ataaagtttt aaacaaaaag
     4621 ctaggtctac ccataactgt ttaagtgtca aaagatatag tgataattta tgaccataac
     4681 atttttaata catgcggaaa atgttcatat tctaaagcat gtaccttttg agcaacatca
     4741 tctggagtat ctgttgacaa tacgtcacac gtagcttgaa aaattatttc accatcatcg
     4801 tacttattat ttacaaaatg tatagtaatt ccagtttgtg tttcatgagc atctaccact
     4861 gcttgatgta cataatctcc atacatcccc ttgcctccaa atttaggtaa caaagcaggg
     4921 tgaatattta caattttatt tggaaattgt gtaataatat tatctgatac tttcaataaa
     4981 aaacctgcta ggataataaa atttatcttt tcatccataa gaaaagacaa cacagttcca
     5041 tcttcgaact gttgtttggt aaatgtatag gaaggaatta aaagtttttt ggcacgttga
     5101 tgcacaaatg catcttgttt attcgaaaga atacatgaaa aattaaccaa ctgactacct
     5161 gtaaaataac gaattatatt ttcagcatta cttcctgaac cggatgcaaa aatggcaaca
     5221 tttatcatat aaaaaaggca cttatgtttt atactttcaa aagaaaacgc aaaggtacaa
     5281 ttaagaattt agatgcacaa tattaatcac aaaagaaaat ttattaacaa caattcttat
     5341 tttattttaa atatatctaa ttgaaataca aatatttaat aataaataaa taagaattat
     5401 agtgcacaaa aattaaatac gtgtgcacat ttttttgaag aaatatcttg tattcgcaaa
     5461 aaaaagtgta catttgcatc gctaaaattt cacaaattta atttatttat taactaaaaa
     5521 ttaaaagtta tgtctgaagt tgcagaaaaa gtaaaagcta tcattgtaga gaaattaggt
     5581 gttgaagcat cagaagtagt tccagcagct agcttcacaa acgatcttgg tgcagattct
     5641 cttgatacag tagaaatgat catggaattt gaaaaagaat ttggtatatc tattccagat
     5701 gaccaagctc aaaaaattgc tacagtgggt gatgctatct cttacattga agcaaacaaa
     5761 taatttaagt taactttatt catggagtta aaaagagttg ttgtaacagg tttaggtgca
     5821 gttactccta ttggcaacga tgttcctaca ttttggaatt cgttaataca aggtgtaagt
     5881 ggagctaagc ctattacgcg ttttgatgca tcaaagttca aaactcaatt tgcgtgtgaa
     5941 gttaaagatt ttgatccaaa tcaatatttt gacagaaaag aagcccgtaa atatgactta
     6001 ttctcaatgt ttggcatcgt agcagcaaaa gaagcggtag aagatgctgg tattgatgtt
     6061 gagaaagtag acttagatca aattggcgtt atttttggaa caggaattgg tggtataaaa
     6121 acatttgccg aagaagtggc tggttttgtt caaaatgagt ctataccacg tttcaatcca
     6181 ttttttattc caaaaatgat tattgatatt ttacctggtc atgtatctat catgtatgga
     6241 tttcgtggta ttaatttttc tacatcttcg gcttgtgctt caagtacaca tgctttggct
     6301 gatgccttta attatatacg tttaggcaaa gctaacatta tggttgcagg aggctcagaa
     6361 gctgccataa ctgatgttgg tgtaggaggt tttaatgctt tacatgccat ttccacacgt
     6421 aatgatagtc cagaaacagc ttcacgtcct tttagtttat ctagagacgg ttttgtgatg
     6481 ggtgaaggag ccggttgttt gatttttgaa gagttagaac atgctttagc acgaggtgct
     6541 aaaatatatg cagaagtaaa gggtgctggt atgacagcag atgctcatca tatgacagct
     6601 ccacatccag aaggactggg agctataaat gtaatgaaat ctgcactaaa agatgcagac
     6661 atgcaaccag atgaaattga ttatatcaat gttcatggta cctctacccc attaggtgat
     6721 attgctgaaa caaaagcaat taaagatgta tttggcgaac atgcatacaa tttgaatatt
     6781 agttcgtcaa aatcaatgac aggacattta ttaggagcta ccggagctgt agaagctatc
     6841 gcatgcgtac ttagtgtaca aaatgacata gttcctccta ccatcaattt tacagaaggc
     6901 gatgaagatc ctgaaataga ttataaattg aactttactt ttaataaagc ccaaaaaaga
     6961 cctgtaaatg cagttttatc gaatactttt ggatttggtg ggcataatgg aagtatcatc
     7021 tttaaaaaat ttgcgaagta atcacttttt taacgtgtac tagcattgat aaaacaaatt
     7081 attaatagaa taaggttcat ttctttcaca aagaacgaac cttattttgt tttacgtaac
     7141 ctacttggat ttagtcccaa agaaaatagt tattaccaac tagctctatt acatcgttca
     7201 acagggaaac gcaatataaa tggcgttatt atagacaatg aacgattgga atttttaggg
     7261 gatgctattt tatctgctat aatctctgat tatttgtatc aaaaatttcc acttaaaaaa
     7321 gaaggatttt taactaatct acgttcaaaa attgttcaaa gagaatattt agactatcta
     7381 gctgtccaaa tgggtttaga taaactaatg ttttcccaaa ataaacatat tgtctctaaa
     7441 aatgcagttc atgtatatgg aaatgctctt gaagcattaa ttggtgcaat ttacctagat
     7501 caaggatata aaaaatgtca acaattttta gagaaacaag ttattgagaa acatatgaat
     7561 gtgaacgaaa ttgtaaaaaa agagtacaat tttaaatcaa aattaataga atgggctcaa
     7621 aaaagaaaat attccattat attcgtagtt acaaatagcg aagtaaataa agaacaaaat
     7681 ataaccacat tttatactag tgtattaatt gaaaatatag aagttggtac aggttgtggt
     7741 ttaaacaaaa aagagtctca acaaaaggca gcaagagatg ctttacacaa aataaagaaa
     7801 aaagcaactg ggattcttat caatactaaa tataaagagc gtatagaact agaaaatcac
     7861 tctgaattaa aaaaactata gaactaaatc attattatat atgaaagcaa gtaatcaccc
     7921 tatccatttg gctcatttag tatttgaaaa agcaaagcaa tatggagaaa gagcagcgtt
     7981 gcactatcgc aatgacagta caaaaacctg ggttcctatg tcatggaatg aaatagcaaa
     8041 acaagtaact tttattagta aaggattagt ttccattggc ttagaggaaa atcaaaatgt
     8101 tggtattttt tcacaaaata tgtgcgaaat tatcattgca gattttgcaa tcatgggtaa
     8161 ccatgcagta tcagtacctt tatatgctac ttctactgct ccccaaatag atttcatcgt
     8221 aaaagatgct gaaattggtc tattgtttgt aggcgaacaa taccaatatg atgtagcgat
     8281 ggaagtaaaa aagcactcaa aatttttaaa gaaaattatt gccattgatc caaatattga
     8341 tttaaaaaac gatcccgaaa atatgtactt ttttgattta cttttacttg gacaaactgt
     8401 taacaatgat gatatagttg agaaaagaaa atcaaaagca tctgaaaatg atcttgttaa
     8461 tatattatac acttctggta caactggcgt tccaaagggt gttatgttac atcaatataa
     8521 ttattctgta gctattcgca ttcacaaaga aagattggct gaggttactg ataaagatac
     8581 ttcgatgtgt ttcttgccca tcacacacat atttgagcga gcatggactt atttgtgttt
     8641 agaacgagga gttcaagtat atgtaaataa acgtcccata gaaatacagc aaacaatact
     8701 cgaagtacaa cctacattga tgtgtacagt gcctcgctat tgggaaaagg tttatgctgc
     8761 cattctcgaa aaaatggaaa cactttctcc agctttgaaa aaaatattta catgggctat
     8821 tcaagtgggc aaggtgtata atttagagta ttaccgccat cgcaaatctg caccaatctt
     8881 actacgatta aaatatatct tggcagaaaa actggtattt aataaaataa aaagtgcagc
     8941 tggtcttact aatttacatt tctttccttg tgcaggatct cgtttagctg atgatattaa
     9001 cgagtttata catagcctcg gaattaattt ggtatatggg tatggtttaa cagaatcaac
     9061 tgctaccgtt tcatgttatt atcctgaaaa taaagaatat ataataggat ctattggtaa
     9121 aattatgcct gaagtagatg ttaaaatttc agatcaaggc gaaattttac tcaagggaag
     9181 taccataacc gcaggttatt ataaacgtcc tgatgctaat gcagaatctt ttgaagatgg
     9241 ctggttcaaa acaggagatg caggctatat tgatgctgct aataatttat atattactga
     9301 tagaataaaa gacctattta aaactgcagg aggtaaatac attgctccac aaatgctcga
     9361 aagccgatta tctgaattaa aatttgttga ccaagttgct attattggcg atcaacgcaa
     9421 atttgttaca gcccttattg tgccaacatt tgatacatta aaaatctacg cttctaatca
     9481 taatataccc tatcaatcaa tagaagagct tatttctcat cctgatatta ttaaatttta
     9541 tagcgattgt attaaagaac aaatgaaaga tttggcaagc tacgaacaaa taaaaaggtt
     9601 taccctactt ccaaaggcat ttacgatgga agacggatta cttacaaata ccttaaaatt
     9661 aaaacgaaaa gaagtagcaa aagtattcga aaatataatt gaaaaaatgt atattgatta
     9721 aaaaatgata ctagaaaaca gactccacat cacaataaaa ttgtatcttt gtgggtttga
     9781 aaaaatattc aataaatatg ataacaatag aacaactaaa agagacgata gagcgccaac
     9841 aagcgttgag gaggtatctt tgacattgat gcaaaattag tagccttaca agaagaggaa
     9901 ttacgaactc aagacccttt gttttggaat gacagcaaaa gtgctgaaat tcaaatgaaa
     9961 aagataaaag aattaaagtt ttggatagaa acatataata aagtttacca agctactgaa
    10021 gagctgaaac ttgcatttga tttctacaaa gaagctatcg tcgctgaaca agacgttgat
    10081 gaggcttata gtgtagcaat ggattgcatt agcaatttgg aaatgaagaa tatgctttca
    10141 gctgaagaag acaatatggg agctattatt aaaatagttt ctggtgctgg aggaactgaa
    10201 agtcaagatt gggcatctat gctactacga atgtaccaac gatggtgtga acgaaatact
    10261 tataagtgtg aacttaccaa tttccaagca ggagatgaag cgggaatcaa aacagcaaca
    10321 cttcaagtag agggagaatt agcttatgga tatttaaaaa gtgaaaacgg agtacaccgt
    10381 ttagtccgcg tatctccatt taatgcacaa ggaaaacgta tgacatcttt tgcttcagtg
    10441 tttgttacac cactcatcga cgactccatc gaaatagata tcaatccagc agatataact
    10501 tgggaaacat tccgttcggg cggtgcaggt ggacaaaatg taaataaggt tgaaactggt
    10561 gtacgtttac gttactggta caaagataca cccactagtg aacccataga aattatcatt
    10621 gaaaatacag aaagtcgatc gcaatttggc aataaggata atgctatgcg attactaaaa
    10681 tcccagttgt acgaaaaaga attagaaaaa cgcagagccg cttctgctca aattgaggca
    10741 ggaaaaaaga aaattgaatg gggctcacaa atacgtagtt atgtgtttga tgatcgacgt
    10801 gtaaaagacc atcgtagcaa ttatcaaaca tctgatgtac agggagttat ggatggtgat
    10861 attgatgctt ttatcaaagc atatttgatg gaattttcta attaataaca gtaaataaca
    10921 atatatgagt ggaatagaat tatctgaaca agaaatcttt cgtcgtcaaa gtttagaaca
    10981 aatgcgttca atgggaattg aaccataccc tgcagaagaa tttccaacaa acgctttttc
    11041 tactgaaatc atttctaatt ttaacgaaaa tgaaaaacgt gatgtggcaa tagctggtag
    11101 aataatgagt agacgcatta tgggcaaagc ttcctttatt gaattacaag attcaaaagg
    11161 cagaatacaa gtatatatct cacgtgatga aatttgtcca gaagaagaca aggaattata
    11221 caatactgta tttaaaaaac tattggatat tggtgatttt ataggtatta aaggatatgt
    11281 ttttcgtaca caaatgggag aaatatctgt tcatgcttct gaactcaaag tactagcaaa
    11341 atcacttcgc cccttgccta ttgttaaaat aaaagatggt attgcgtacg atgcttttac
    11401 tgacgcagaa tcaagatatc gtcaacgata cgttgattta gtggtaaacg aacaggtaaa
    11461 agaaattttc ttgaaacgta ccaacgtatt taactctatg agagaattta ttaatcagca
    11521 tggctatatt gaggtggata cacctgtttt gcaaccaatt ccaggtggag cagctgcacg
    11581 tccatttact actcaccata atgcattaga tataccatta tatttgcgta tagctaacga
    11641 attatattta aaaagactca ttgttggtgg ttttgaaggg gtatatgagt tttcacgtaa
    11701 ctttcgtaat gaaggtatgg acagaacaca caatccagaa tttactgtaa tggagctata
    11761 cgtatcgtat aaagattaca attggatgat ggattttacc gaaaaaatgc ttgaaaaaat
    11821 ctgtattgat gtaaatggaa caaccgaagt tccgatggga gaaaaaataa ttaattttaa
    11881 agccccattc aaacgaattt caatgaccga tgctattaaa gagcatacag gttttgatat
    11941 aacaggtaaa aatgaaaaag aattacgtga aatatgtaat gcactccaca tagaaaccga
    12001 tgaaacaatg ggcattggaa agcttattga cgaaattttt ggggaaaaat gtgaaggcaa
    12061 ttatattcaa cctacattta ttacagacta cccaattgaa atgtctcctc tcacaaaaaa
    12121 acaccgttct aaagcaggat taaccgaacg ttttgaatta atggtaaatg gaaaagaact
    12181 tgcaaatgct tattctgaac taaatgaccc tattgatcaa cgagaacgat ttgaagaaca
    12241 acttcggtta agtgaaaaag gtgatgatga agctatgttc atcgatcaag attttttaag
    12301 agcaatggaa tatggaatgc cacccatgtc tggtataggt ataggaatgg atcgattagt
    12361 tatggctatg accaatcaaa ccagtataca agaagtatta ttatttccac aaatgcgtcc
    12421 tgaaaagatt gctaaaaaag atacccctgc acgatttata gaacttggca tgtctgaaga
    12481 atgggcacaa gctatagtaa aacattcgtc tctttctatc aaagatattg caagcaatat
    12541 tgatgcaaaa ttgcatcagc aactgtgtgg tatcaataaa aaacacaaac tatccttacc
    12601 taatcctact ttagcagacg tacaagtctg gataaaaaaa gcaagcgaat aacagtatgg
    12661 aacaaatgaa agcaaaacga atatgcatta tgggaggtgg aagctgggca accgctttag
    12721 ccaaaattac catgtcaaaa ggagataaaa ttaattggta catgagaaga cctgagcaaa
    12781 ttgaaatatt ccaacgctta ggaaataatc cttcctatct cagttcgatt agatttgata
    12841 caaaaaaaat aaaattttat gcagacatta acttagcagt taaaaactct gatattttag
    12901 tttttgcaac tccctctcct tttcttaaac aacatttaca aaaactaaca cgtacattaa
    12961 gtaataaaat aatcgtatct gcaatcaaag gaatagttcc tgatgaaaat atgattatta
    13021 atgattattt tcaaaaattc tatggtgtta aacaagaaaa tattgctatc attgctggac
    13081 cttgtcatgc agaagaagtt gctctagagc gtctttcata cttaaccatt gcatgtgcta
    13141 ataacgaatt aggaacatat ttagccgaaa gattaagttg ttcattctta aaaacatcta
    13201 ttacaagcga tattaatggc attgaatatt catctgttct caaaaatgtt tatgctattg
    13261 cagtaggaat atgtcatgga ctaaaatatg gtgataactt tcaggcagta ctaatttcaa
    13321 atagtattcg tgaaataaat cgttttgtag atgcaataga acggaataat gagcgtaata
    13381 cgcaagattc agcctattta ggcgatttaa tagtaacagc acattctcgt tttagtcgta
    13441 atcgtacttt tggcaccatg ataggaaaag gatattctgt aaaaacttca caattagaaa
    13501 tggaaatgat tgcagaaggt tattatggaa ctaaatgtat taaagaaatt aatgatactc
    13561 taaatgtaga tatgcctatt cttgatgcag tatataatat attgtacgaa cgcatagccc
    13621 caacaatcga aattaaatta ttaacagaaa aaatacgata aaaatggaaa acattaaatt
    13681 ggtaattgac aataccttag gttttatatc aaaagataaa gtatttgctt acgaaaaaga
    13741 agtaagtgca tgcaatagta tgctacacaa tggtacagga aaaggaaatg attttttggg
    13801 atggttatcc cttccatcaa gcacttctgc tgaattaatt aacgatatca aacaaactgc
    13861 tgatttatta cgtaatgaat gtgaagtagt agttgtagca ggtattggag gaagcttctt
    13921 aggtgcaaaa gctgtgattg atgctttgtc taacacattt atttggttga aaaaagacac
    13981 aaatacaccc gtaattgtat atgccggtaa taatattggc gaagattatt tagctgaatt
    14041 aatggattat ttatcagata aaaaatttgg tatcattaat atttcaaaat ctggtacaac
    14101 taccgaaact gctttggctt ttcgtttatt aaaaacacaa ttagaaaaac aggttggcaa
    14161 agctaaagct aaaaacttaa tagtggctgt aactgatgct tcaaaaggtg cattgcgtac
    14221 attggcaaca caagaagggt acaaaacgta tgtaattcca gataatgttg gtggtcgttt
    14281 ttcagtactt tatcctgtag gacttttacc aattgctgta gctggattag atttaaatca
    14341 attgttggct ggggctagaa aaatggaaga agtgtgcgga ttaaatactc cttttgctga
    14401 aaatcctgct gcaatatatg ctgctacacg taatgaatta tatagaaacg gtaaaaaaat
    14461 tgaaattcta gctaattatc aaccaaaact gcatttcttt gctgaatggt ggaaacaatt
    14521 atttggtgaa agcgaaggaa aagaaaataa aggtatattc aatgctgctg tcgatttttc
    14581 aaccgaccta cactcgatgg gacaatggat acaggaaggc gaacgtacta tttttgaaac
    14641 agttatctca attaaacaag cttcaaaaaa ggtagttgtg ccaacggata acgacaactt
    14701 agatggattg aactttttag cagggaaacg tgttgatgaa gtaaacaaaa tggctgaatt
    14761 gggaactatg ttggctcatg ttgatggcgg tgttcccaat atgaaaattg aggttcctga
    14821 attaaatgag tattacatcg gacaattaat atatttcttc gaaaaagcat gtggtattag
    14881 tgggtattta ttaggcgtaa acccatttga ccaaccagga gttgaagcat ataaaaagaa
    14941 tatgtttgcc ttacttgaaa agccaggttt tgaggcagaa acaaaagcta ttaaagcaag
    15001 actataatat ggaatacaca gggaaagaaa tcattaaaac tttccctatg tactataaaa
    15061 aagcgataaa agaatttgca aatacaaaaa aataacttac ctttgcatcg cttttgaaag
    15121 aaaaggggcg tttagctcag ctggttcaga gcatctgcct tacaagcaga gggtcggcgg
    15181 ttcgaatccg tcaacgccca caaccttttc ttttaaaata caatataata cgggcgttta
    15241 gctcagctgg ttcagagcat ctgccttaca agcagagggt cggcggttcg aatccgtcaa
    15301 cgcccacaca accttaaagt ttgtcaatta tgacagactt ttttgtttta tactacgcta
    15361 tttcgtactt ttagcataaa aattttacta tgctcaaaag attaaaaacc catcttactt
    15421 tcttatgctc gtttattctt gtggcacacg tatatagtgc tcaatatcaa ggaataatac
    15481 tagatagtca aagccataaa gcaattcctt acgtaagcat acatacatca aataataatg
    15541 atatttatgg aagtatgagt aatgatttgg gcgaattttc aatctctttt gactttgatt
    15601 cacttgtatt tcaacatatt aattatactc catacatttt aaaaaaacaa tcgtataagg
    15661 acacactata ccttaatcct ctatcttata ctctaaatga aattgatgtt tatgctacag
    15721 aaagcctctg gattagaaaa aaactacacc ttgttgtaaa aaatataaaa aagttctata
    15781 cactaccctc gtctattcaa caagattatg aatataacac atatacctta tcagactcaa
    15841 gtggttatgc attcgagagc aaaggtatat tacaatccaa taaaaatgaa tactcaattt
    15901 taccaatact ttctgctata aaatataagg attcgacagc aggtactgat tttagtaatc
    15961 tgagaagaat gttacacgat gattttttac cacgcttcac ttcaaacttt attaagaata
    16021 attcatttcg tgagaacaaa ggatttaggc ataccaatcc gaatgttgta caattgtttt
    16081 atttttcaaa tgataacctt agtgatgacg gatatttggt aatagacacc ttgaaaaatg
    16141 taatactcga agctcaacaa aacttaggta ctcaaagtaa tatagaaaat aatacttctt
    16201 ctttattgcg taaagcagct gaattaatag gttttactta caatgattgg attacgcaaa
    16261 cccagtatat ctatacagaa attaacggct catacctact acaatcctgt atctacaagc
    16321 tttatatgcg tacaacaact gaaaataaga aactatcaaa ctcgtatttt gtttcaacag
    16381 aatcaacagt tcgtttgagt aataccactc taacaaattc tgtatcaaat agtaagcaat
    16441 ttttgatttt acctaaacct tattacatcg taccaatcta tacaaaaacg atgagagaag
    16501 aagaagaaaa attgcaacga ataaaaagga cgtataaaaa attttaaaaa tagtgttatc
    16561 taaaatattt ttgtgtactt atgacaatat ggtgtacctc ctgttttagt ataatatgtt
    16621 tgatgataat cttctgctac ccaaaattct tctgcttggc gtaactgtgt agctacaaag
    16681 tatccttttg tttgcaacaa tgtgatataa tatgtggcta tatctctttg tttttcattg
    16741 gtatagaaaa ttacagaaac gtattgcgac ccaatatcag gtccttgccc attaatttgc
    16801 gaaaaattat gtgtttcgaa gaataattta acgagtgtct catattgtag ttgttcatta
    16861 tcataaacaa tttctactgt ttctatgtgc cctgtatctc ctgtgcaaac ctctctataa
    16921 gttggagcaa atgtcagccc acccatatat cctacttgag tcgcaattct tgttagttgc
    16981 ataaaatagt attcagtacc ccaaaaacaa ccagaagcaa aataggcttt ttctatcatt
    17041 attttttcat caattttatt gataatgaat ttacacaatg tcttacattc ttttcagtaa
    17101 aaccttcacc ttcaaataca tgtcctaaat gtccatgaca atttgcacac acaatttcag
    17161 ttcttctacc atccatatcg ggaatttcca acacagcttc ttttatagca tcatcaaaac
    17221 ttggccaacc acattccgaa tcaaatttat catcagacaa aaacaatggc gtatcgcatc
    17281 tacgacaact atatatacca tcttctttgg tatgtacata ttctccgcta aaaggtaatt
    17341 cagtgccttt atgcactata atgtgttctt cctctttagt tagtttgttc atattattaa
    17401 aaaaaatgct aatttttcaa ttagcattta at
//
LOCUS       DAOSRP010000022        17372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3408_length_17372_cov_5.397355, whole genome shotgun sequence.
ACCESSION   DAOSRP010000022 DAOSRP010000000
VERSION     DAOSRP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17372
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_17372_cov_5.397355"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLJ82_02505"
     CDS             <1..723
                     /locus_tag="PLJ82_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40412.1"
                     /translation="MYEYVGVKTLTEQNIIEKDAIFIPGHSGDFLAGSQLQKAGITLE
                     TTLNEVEKLVLEQKFAYGNGLKNAAIKQKMNDFIKQRKDYLPYSVFDDVDLLIKLPKN
                     INNAARIYGFFGHQTRLPFWDNEMIEFFKTLPIEEKQTQKFYHHYLTSKVFQPMNVAF
                     VDELTYPEWRMKWYRFKRQCKQLFPTHIQQYISNPPDYVCMKEISAPLKHQLPKTATN
                     GMGGYNRVFLEWYWQQVKKIVD"
     gene            complement(729..1703)
                     /locus_tag="PLJ82_02510"
     CDS             complement(729..1703)
                     /locus_tag="PLJ82_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40413.1"
                     /translation="MEKVSKQEFYSLIKGFEQIPFTQTEAWVTAHSRHDDTKLAFFVD
                     QLPEPLIACVAHVKKKAGHKMYYIEAEAIRYKDIKHAKLTSFFQDLQQTGVDVIEINS
                     PMPYDATYEIALRQAGFLRPVGQFSVALSSYISLENEIKYNDNWKRNMKKAANNQLSF
                     EVIDNAVDDDINDFIKLYNEMSVQKNVAPTFTFSSLRTLLTSANFKLFFVKNEQNERI
                     ATILIHYNNNHAGLVYAASGEKAHQCAASFFMYDKLFHYLSQQHIKTFDMEKLAPSIH
                     STNAVFLFKNGIKGEYIVVNGEWSWHKNSFLRPLMYVAKKWFLKKIEW"
     gene            complement(1691..2659)
                     /locus_tag="PLJ82_02515"
     CDS             complement(1691..2659)
                     /locus_tag="PLJ82_02515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR40414.1"
                     /translation="MTNKEKYSQLCTENDTIPLFLQHWWMEAVCTKASKEWDVLLYEK
                     NKKVVAALPYHFITKLGMKVILQPQLTQYSGVWIDYPNGITDKERYSLEEEVCKGLIS
                     QLDTLSWVHYMQHFHYRFTNWLPFFQQGFTQTTRYTYRIESIGNTQDVFDNFHQGKQK
                     HIKKAKDLSVDYSLSAADFYAFLSHTIAQKQQQVVYSQSFFVGLHQQAVEREQGQIIA
                     VHDKTGAIHAAMFVVWDHLSGYYLVSAIDANYKHSGASSLMLWEAICFLSSRTKSFDF
                     EGSMIKGVAKANQEFGGVQTPYFSISKSRSGVLGCMFNVYRWLWKK"
     gene            2724..3089
                     /locus_tag="PLJ82_02520"
     CDS             2724..3089
                     /locus_tag="PLJ82_02520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOR40415.1"
                     /translation="MNIFLQKQARSFRNAFRGIVVAIQQETHCKIHLLATIAVIVLGI
                     LLHIALNEWIVIFICIAMVWCLELINTAIENIVDMVSPERQERARNIKDIAAGAVLFA
                     AICSMIIACLIFIPKIISW"
     gene            3083..3787
                     /locus_tag="PLJ82_02525"
     CDS             3083..3787
                     /locus_tag="PLJ82_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOR40416.1"
                     /translation="MVNYLQHYISGFISLIYPQVCIVCGNHLVQSEKSICQQCVIDLP
                     KTGFHLLQNNPVEKLFWGKIHVEFASAFFFFQKGSVAQKLLHNLKYKGNKEIGIELGK
                     HYATELVRSSLIDTIDYIVPVPLHKNKLKKRGYNQSEMIAIGLSQVLSIPVETDTLVR
                     AIENPTQTKKGVFERWENTYGVFTITNTQQFANKHILLVDDVLTTGSTIEACVQAIKQ
                     APNAKVSFISLALASN"
     gene            3800..4534
                     /locus_tag="PLJ82_02530"
     CDS             3800..4534
                     /locus_tag="PLJ82_02530"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOR40417.1"
                     /translation="MIETIHIHETTSTNDLLNQLMREKSLEEGSMVYTDFQTLGRGVS
                     NNVWESEKGENLLCSILLKPSFIPIKQQFIISQVVSLALTDFLLPYDTEFTIKWPNDI
                     YWKDNKIGGILIENNLENGLLASCIIGIGLNINQKHFYSSAPNPISLIQITNTFHPIK
                     EIATSIRNAIFERYIHLLKGKTKELQQTYTDRLYRSKGLFRFQDKDGIFVASIFQVTP
                     DGYLELTKENGDQQRYAFKEVSFIIP"
     gene            complement(4524..4739)
                     /locus_tag="PLJ82_02535"
     CDS             complement(4524..4739)
                     /locus_tag="PLJ82_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOR40418.1"
                     /translation="MSFLFGILFFILAIGLFVLVFFFSILRNVFRLFTGKQHPEKKEQ
                     ERMARKYVNKKITKQQGEYVDFEEIKE"
     gene            complement(4746..5540)
                     /gene="lgt"
                     /locus_tag="PLJ82_02540"
     CDS             complement(4746..5540)
                     /gene="lgt"
                     /locus_tag="PLJ82_02540"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR40419.1"
                     /translation="MALATIIWDVNPEAFSIGFITVRWYGLLYAMGFLLGITILGKIF
                     KRENCPEDWADKVFIYMVLAVVIGARLGHVFFYAWDYYKDNLLEIVMIWHGGLASHGG
                     AIAMLIAAWIFSKKVSKKSFLWLGDRLFIPSAMVAGLIRIGNLMNSEIYGHATDLPWG
                     FVFVRDGGTMPMHPTQIYEALAYFALFAFMMYLYWVKDAHKREGLLTGVGFTGIFVAR
                     FFIEFVKNNQEAFEDDLMLNMGQYLSIPFIAIGIWLIIRALRSNKI"
     gene            complement(5572..8859)
                     /gene="secA"
                     /locus_tag="PLJ82_02545"
     CDS             complement(5572..8859)
                     /gene="secA"
                     /locus_tag="PLJ82_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR40420.1"
                     /translation="MGLNSILSKLFGSKSQRDLKEIMPFIEKIKAIYPQVEQLSNDEL
                     RNRLQGVRLEIQQTVSDERERIATLKQSIDATSIEHRDKLYNEIDAIEKAIITKLEEA
                     LDNALPEVFAIVKDTARRFKENETIEVEATDFDRELAVTHDFVDIVSNKAIYKNEWEA
                     GGNVIKWDMVHYDVQLIGGVVLHKGKIAEMTTGEGKTLVATLPVFLNALSGNGVHVVT
                     VNDYLSKRDSEWMGPIYMFHGLRVDCIDKHRPNSDDRRKAYLADITFGTNNEFGFDYL
                     RDNMASTPEDLVQRKHNYSIVDEVDSVLIDDARTPLIISGPIAKGEEQLYEQYCSKVE
                     RIVAEQKNLVTKTFVEAKRLMQSADAKEQEKGSLLLFRAFKGLPKNKALIKYLSEPGV
                     KASMLKTEAIYMEQNNRRMHEVTDDLYFVINEQHNTIELTDKGIDLITGDSDDALFFV
                     LPDIGSEMAEIEKAKLSDEEKQAKKDVLMQEFSIKSERVHTINQLLKAYTLFEKDDQY
                     VVMNNKVMIVDEQTGRIMDGRRYSDGLHQAIEAKERVKVEAATQTYATITLQNYFRMY
                     NKLAGMTGTAETEAGEFWDIYKLDVVVIPTNRPIARVDMNDRVYKTARAKYNAVIEEV
                     EKLVAQGRPVLVGTTSVEISELVSKMLKLRKIPHNVLNAKLHQKEADIVAQAGKSGTV
                     TIATNMAGRGTDIKLTPESKAAGGLAIIGTERHESRRIDRQLRGRSGRQGDPGSSVFF
                     VSFEDSLMRLFGSDRIVGIMDRLGLQENEMMEHRQISNSIERAQKKVEENNFGIRKRL
                     LEYDDVMNSQREVIYARRRHALIGERIGIDIVNMIYDGVENLVNQHDDIQDEEEFRLD
                     CLKLFGVDIQLEEGEIKRTKTIDLVEKVFSLVMANFSRRADKLAEIAYPVIKRVYEEQ
                     GKIYENILIPLTDGKKLYNIPVNLKLAYETQAKEIVKSFQKSILLHTIDEAWKEHLRE
                     LDELRNSVQNASYEQKDPLLIYKLESFGLFKKMIDAINYKAIAILMRGQIPVKEGETL
                     REAKEERRQDFSKYNTQKSDIMNAGQQDTREKIHKEPVRVEKKVGRNDLCPCGSGKKY
                     KSCCGKV"
     gene            complement(8998..9882)
                     /locus_tag="PLJ82_02550"
     CDS             complement(8998..9882)
                     /locus_tag="PLJ82_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR40421.1"
                     /translation="MSYHFLRLIVFSFVVSITNSALAVTLHDNIFRIEDPKNASSSLM
                     LLNENTEDDFADVDDFENFDEISFNAPYNSWSNTAVNPYNINLLHSKDTFRVDVSGYT
                     HPLDKVQRITSHFGPRRTRYHYGIDLKLNVGDTVRSSFDGMVRIAKIGRGYGYYVLVR
                     HFNGLETIYGHLSKILVDTEQLVKAGDPIGLGGNTGRSTGPHLHYEVRYLGNAINPES
                     LICFDNLTVKTPELLVSAETFRYKLDVDRMRNYTVRRGDTLSTIARRQGTTIRNLCRL
                     NNITTKTTLRVGRVLRIS"
     gene            complement(9893..10357)
                     /gene="ndk"
                     /locus_tag="PLJ82_02555"
     CDS             complement(9893..10357)
                     /gene="ndk"
                     /locus_tag="PLJ82_02555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOR40422.1"
                     /translation="MMEKTLVIIKPSAISRGLIGEVISRFEKKGLHLIGMKMIELSDE
                     ILAEHYAHLAQKPFFQRIKNAMQTTPVIVCCWSGLDAVQVVRNMAGVTNGRNAASGTI
                     RGDYSMSVQENIVHTSDSVENAAIELNRFFKPEEIYNFPPHSLDFLYANDEL"
     gene            complement(10440..12530)
                     /gene="recG"
                     /locus_tag="PLJ82_02560"
     CDS             complement(10440..12530)
                     /gene="recG"
                     /locus_tag="PLJ82_02560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOR40423.1"
                     /translation="MDLTKSDIKYLHGMGPKRAELLNKELEIYTLDDLVHYFPYKYID
                     RSRFYQISEIDSTAAYIQIKGQILGFKEAGKGRAQRLIAIFTDGHSSIELIFFKGIKY
                     VMDAYHLNTDYIVFGKPNFFNGNYSFVHPDIEKPTPDSMTGFMPLYHTTEKMKNKFLN
                     SKAIQKLIYQALQTITPSLPESLPTSILHKYRFISLYEALINIHFPSNMPLLEQAQAR
                     LKFEELFYIQIAILRQAHALKTSFKGFQFNHIGEMFHAYYEKCLPFELTNAQKRVLKE
                     IRNNVRGAYQMNRLLQGDVGSGKTIVALMCMLMAIDNGYQACIMAPTEILARQHFETI
                     ESQINKLPIQVKLLTGSTKKKDREHIHEGLLNGSVHILIGTHAVIEDTVNFKNLGIVV
                     IDEQHRFGVAQRAKLWNKNTRPPHVLVMTATPIPRTLAMTVYGDLEVSVIDELPPGRK
                     PIVTNHIFEDTKREALNRFLKQQLSLGRQVYIVYPLIEESEKMDLKNLTDGYQYIQSI
                     FPDLQVSMVHGKMKPAEKDAAMQEFVQHKSQIMVATTVIEVGVNVPNASVMVIENAER
                     FGLAQLHQLRGRVGRGADQSYCVLVTSRKISNETRKRLEIMTRTNDGFEIAEADLQLR
                     GPGDLEGTQQSGLPFQLHIANLAKDGHFVQLARECASEILENDPQLKSPANEIIVKEL
                     KTKKREQQNWSIIS"
     gene            complement(12511..13182)
                     /locus_tag="PLJ82_02565"
     CDS             complement(12511..13182)
                     /locus_tag="PLJ82_02565"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR40424.1"
                     /translation="MEKYIIIVAGGTGNRMGANVPKQFLLLNGEPILARTLRAFANWG
                     NIILVLPETQMTYWQSLCKNLRDMPDYKMVAGGEQRFYSVYNALQFVPDGALVAIHDG
                     VRPLVSQTCINRVFTLAEEKGSALPVINCVDSLRHISNGSSLSVNRNEYVCVQTPQVF
                     NSTNLKKAYQQPYKECFTDDASVYESATYKINLCDGNIENIKITMPIDLIHAEAILKN
                     GFDKV"
     gene            complement(13546..14892)
                     /locus_tag="PLJ82_02570"
     CDS             complement(13546..14892)
                     /locus_tag="PLJ82_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR40425.1"
                     /translation="MAHLLKRKIDTFLAEWKHNNAHLPLIVKGARQVGKTASIMAFAE
                     ANYESVIAINFALQPKFKNICSDGFDVDSILKNISFLLPDSKLPQKKTLIFFDEIQAY
                     SACATSLKSFALDGNYDVICSGSLMGINYNEIESNSVGYKEDYEMHSMDFEEFLWAKS
                     YSAQQIEGLFNKMIELKPLSTVEVSVLSDIFRDYMITGGMPAIVNNYIKNNNFSDTLG
                     IQKQLLLDYEEDITKYAIGLDKGKIKSIYNHISIFLGKDNKKFQITKIAKNARNRDYI
                     GTVDWLNDAGIINICYCLDSVELPLKGNNNPKSYKLYYKDTGLLIAALDDESQEDLRA
                     NRNFNTYKGAIYENIVGDMLVKQGYQLYFYKNESSNLEMDFFVRDRDSLIPIEVKAND
                     NSTRSLNNLLDDNIKFKDIKFGIKLCNRNIGFNGKFYTFPYFLSFMIKRFLQDRNQ"
     gene            complement(15180..15962)
                     /locus_tag="PLJ82_02575"
     CDS             complement(15180..15962)
                     /locus_tag="PLJ82_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOR40426.1"
                     /translation="MAIYDDNNLRDTLEYLYDKYNREEFIEHDPISIPHKFDRQEDIE
                     ISGFLAAAIAWGNRKAIVKSANRMLTYLDNKPYDFVLNASDAEIKNLYSFVHRTFNGR
                     DFADFIKSLRHFYLKYGSLGNFFEENYLQSNDIRIVLSKFRAEFMCVEHNDHCDKHIS
                     SIDKASACKRLNMYLRWMVRRDDRSVDFGLWKRIPMSSLYIPLDVHSGNTGRELGLLQ
                     RTQNDWKAVEELTGSLRKFDASDPVRFDFALFGVGVDKRVNG"
     gene            complement(16057..>17372)
                     /locus_tag="PLJ82_02580"
     CDS             complement(16057..>17372)
                     /locus_tag="PLJ82_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOR40427.1"
                     /translation="AAMAVISATALGAGTYCDYAVTGGTMSYETTATNTYKVTYTSTS
                     VLTSVANCNFYYRDNGGNGVQMSFSKVDVDGYVWAATFVTTYTAGDLTYANYCAFNKS
                     GGGLVEVTFPTGTIANYAGTCSSVTVTIGEKTSTYNSVTLNWSVSGGTPTAQTVRYKE
                     SGTETWSNGPSVLANATSATITGLDASTTYDLKVIATVSSVDYEDTDTKATTNPTTCS
                     GKSQVKNTLDGSLKNNCDFTVPYEFEITTSDLTENDPTVLVRYKYTQSQSIDNNQVKL
                     CRMFEEGQIESELQMTAEAGGTGWWYINLEGETLGNLSVGDSAKFAIKVVPAGGCTGG
                     TGTFYATRMFAYKVGVGCNDARAFEFTKRLASQTTFFIQTDASIQRILVNKKGSSEYI
                     QDNYIEPAASKFALDISGYELGTYEFEIFDESGRSDDIKIIHTVY"
BASE COUNT         5436 a         3458 c         2762 g         5716 t
ORIGIN      
        1 atgtacgaat atgtaggggt aaaaacgctg actgagcaaa acatcattga aaaagatgcc
       61 atatttattc caggacattc tggcgatttt cttgctggta gtcaattaca aaaagcggga
      121 attacgctgg aaactacatt gaatgaggtt gaaaagctag tgctagaaca aaaatttgcg
      181 tatggcaatg gcttgaaaaa cgctgctatt aaacaaaaaa tgaacgactt tatcaagcaa
      241 cgcaaagatt atctacccta tagcgtgttc gatgatgtag atttgcttat aaaattacct
      301 aaaaatatca ataatgcagc acgtatttat ggcttttttg gtcatcaaac tcgcttgcct
      361 ttttgggaca atgaaatgat tgaatttttc aaaacattac ccattgaaga aaaacaaaca
      421 caaaaatttt accaccatta ccttacaagc aaggtatttc agcccatgaa tgttgcattt
      481 gtagatgaat taacataccc agaatggcga atgaaatggt ataggtttaa aaggcagtgc
      541 aaacaattgt ttcccactca cattcagcag tatatttcca atccgcctga ttatgtgtgt
      601 atgaaagaaa tttctgcacc attaaaacac caactaccaa aaacagcaac aaatgggatg
      661 ggtggttaca atcgggtatt tttagaatgg tattggcaac aggtaaaaaa gatagttgat
      721 taagtagcct accactctat ctttttcaaa aaccattttt ttgcgacata catcaaagga
      781 cgtaaaaagc tatttttatg ccacgaccat tctccattta ccactatgta ttctcctttt
      841 atgccattct taaacaagaa cactgcattg gtagaatgaa tagaaggagc tagcttttcc
      901 atatcaaaag tctttatgtg ttgctgcgac aaataatgaa acaatttgtc gtacataaaa
      961 aaactcgctg cacattgatg agctttttcg ccagaagcag catacaccaa tcctgcatgg
     1021 ttattgttgt aatgtattaa tatggtagca atacgttcgt tttgctcgtt tttcacaaaa
     1081 aacaacttga aattagcact tgttaacaag gtacgaagag aggaaaaagt aaatgttgga
     1141 gcaacattct tttgaacact catttcgttg tacaatttta taaaatcatt gatatcatca
     1201 tcaacagcat tatcaattac ctcaaaagat aactgattat tagctgcttt cttcatattc
     1261 cgcttccaat tatcattgta ctttatttca ttttcaagag agatatacga acttaaagcc
     1321 accgaaaact gcccaacagg acgcaaaaaa cctgcttgtc gcaaggctat ttcgtatgtt
     1381 gcatcatacg gcattggcga attaatttca ataacatcca ctcctgtttg ttgcaaatct
     1441 tgaaaaaaag atgttagctt agcatgcttt atatctttgt atcgaatggc ttcagcttcg
     1501 atgtagtaca tcttgtgtcc agctttcttt tttacatgtg ctacacaagc aattaatggt
     1561 tctggtagtt ggtcaacaaa aaatgccaac ttcgtatcat catggcgact atgagcagta
     1621 acccaggctt ctgtttgcgt aaaaggaatt tgttcaaacc ctttaattaa actataaaac
     1681 tcttgtttgc ttactttttc cataaccatc gatatacatt aaacatacaa cccaacacac
     1741 ccgaacgaga cttgctaatg gaaaaatacg gcgtttgtac accgccaaac tcttgattag
     1801 ctttggctac tcctttaatc atacttcctt caaaatcaaa cgacttggtg cgtgaggata
     1861 aaaaacaaat agcctcccac agcatcaggg aagatgctcc tgaatgtttg tagttggcgt
     1921 caatggctga aactaagtaa tagcccgaca gatgatccca aacaacaaac atagcagcat
     1981 gtatcgctcc agttttatcg tgcacagcaa taatttgtcc ttgctcacgc tcaacagcct
     2041 gttggtgcaa accaacaaaa aaggactgac tatagactac ttgctgttgc ttctgagcta
     2101 tagtatggga taaaaaggca taaaaatcgg ctgctgaaag agaataatca actgacaaat
     2161 ctttggcttt ttttatgtgt ttttgttttc cttgatgaaa gttgtcaaac acatcttggg
     2221 tattgccaat agactcaata cgataggtat aacgtgtagt ttgtgtaaac ccttgctgaa
     2281 agaatggcaa ccaattggta aatcgataat gaaaatgttg catataatgc acccacgata
     2341 gtgtatccaa ctgacttata agccctttgc aaacctcttc ctccagcgaa tagcgttctt
     2401 tgtcagtgat accatttgga tagtcaatcc acacccccga atactgtgtt aattgtggtt
     2461 gcaaaataac cttcatcccc aatttggtga taaaatgata aggcaaagct gctactactt
     2521 ttttattctt ttcatacaac aacacatccc actctttact ggctttggta catacagctt
     2581 ccatccacca atgttgtaaa aacaatggaa tagtatcatt ttcagtacaa agttgagaat
     2641 atttttcttt attagtcatt ttataggcag tagtttgtag ctgttgtgca aatttatgta
     2701 tttttgcaga atagacaaca tatatgaata tctttttgca aaaacaagca cgtagtttta
     2761 gaaatgcctt tcgtggcata gtagtagcta ttcaacaaga aacacattgc aaaatccacc
     2821 tactggctac aatcgctgta attgtattgg gaattttatt acatattgcc ttaaacgaat
     2881 ggatagtaat attcatttgt attgcaatgg tttggtgttt agagctaatc aatacagcta
     2941 tcgaaaatat agttgatatg gtttcgcccg aaagacaaga aagggctaga aacataaaag
     3001 atattgccgc aggagcagta ttatttgccg ccatttgttc tatgattata gcttgtctga
     3061 tttttattcc taaaattata tcatggtaaa ttatctacaa cactatatct caggatttat
     3121 atccttaatt tacccacaag tttgtatcgt ttgtggaaac cacttggtac aatcagagaa
     3181 aagcatatgc caacaatgtg tcatagatct tccaaaaaca ggatttcacc tactacaaaa
     3241 caatcctgtt gagaaactat tttggggaaa aatacatgtg gagtttgcat cggctttttt
     3301 cttttttcag aaaggaagtg tggcacaaaa gttactgcat aatttaaagt acaaagggaa
     3361 taaagaaata gggatagagc ttggcaaaca ttatgcaacc gaattagtga ggtctagctt
     3421 aattgatacc attgattata ttgttcccgt tcctttacat aaaaacaaac taaaaaaaag
     3481 agggtataac caaagtgaaa tgattgctat cggattatca caagtgctct ctattcctgt
     3541 tgaaacagac acactggtaa gagccatcga aaatccaaca caaacaaaga aaggtgtgtt
     3601 tgaaagatgg gaaaatacgt atggtgtttt tactataacc aatacccaac aatttgcaaa
     3661 taaacatatc ctactggtcg atgatgtatt aactacagga tctaccatag aagcctgtgt
     3721 acaagcgatt aaacaagccc caaatgcgaa agttagtttt ataagtttag ctttagcatc
     3781 taattaaact aaaccgctaa tgattgaaac catacacata cacgaaacaa cctcaacaaa
     3841 tgatttactt aatcaattaa tgagagaaaa atcactagag gaaggttcta tggtatatac
     3901 agatttccag acacttggaa gaggcgtttc gaataatgta tgggaatcag aaaaaggaga
     3961 aaatttgctt tgtagtatat tactaaaacc atcttttatt cctattaagc aacagtttat
     4021 tatttcgcag gtagtgtcat tagctcttac tgacttttta ttgccttatg atactgaatt
     4081 tactatcaag tggcccaatg atatttattg gaaagacaat aaaattggag gtattttaat
     4141 tgaaaacaat ttagaaaatg gattattagc gtcttgtatc attggtattg gtctgaatat
     4201 caaccaaaaa catttctatt catctgcacc taatccaatt tcacttatac agattaccaa
     4261 cacttttcat cctattaagg agatagctac cagcatacga aatgctattt ttgaacgcta
     4321 tattcactta ctcaagggaa aaaccaaaga attacaacaa acgtatacag acagattata
     4381 tcgctccaaa gggctgtttc gttttcaaga taaagatggg atttttgtcg catctatttt
     4441 ccaagtaaca cctgatggat atttggaatt aacaaaagaa aacggagatc aacaacgtta
     4501 tgcttttaaa gaagttagtt ttattattcc ttaatctctt caaaatcgac atattcaccc
     4561 tgttgctttg taattttctt atttacatac ttacgagcca ttcgctcttg ttcctttttc
     4621 tcaggatgtt gttttcctgt aaataaacga aaaacattac gtaatatgga aaagaaaaat
     4681 accaatacaa ataaaccaat agcgagtata aaaaataata tcccaaataa aaaactcatt
     4741 gcatcttata ttttattcga acgtaatgct ctaattatca accatattcc aatggcaata
     4801 aatggaatac ttaaatactg acccatattt aacattaaat catcttcaaa agcctcttga
     4861 ttattcttta caaattctat aaagaaacga gcaacaaata ttcccgtaaa tcctactcca
     4921 gtaagcaagc cttcacgttt gtgtgcatcc tttacccaat ataagtacat cataaaagca
     4981 aacagagcaa aataagctaa agcttcgtat atttgtgttg ggtgcatggg catagtacct
     5041 ccatcacgga caaacacaaa accccatggc aaatcggtag catgaccata tatctcggaa
     5101 ttcatcaaat tcccaatacg aatcaaacct gccaccatag ccgaaggaat aaacagtctg
     5161 tctcctagcc acaaaaaact tttcttacta accttttttg aaaaaatcca agcagcaatc
     5221 aacatagcta tggcaccacc atgactggct aatccgccat gccaaatcat cactatctct
     5281 aacaaattat ctttgtaata atcccacgca taaaaaaaca catgtcccag cctagcacca
     5341 attaccactg ccaaaaccat gtaaataaac accttatcag cccaatcttc tggacaattt
     5401 tcacgcttaa atattttacc caaaatagta atacccagca aaaaccccat tgcataaagt
     5461 aaaccatacc aacgcacagt tataaatcca atcgaaaaag cttcaggatt tacatcccat
     5521 ataatcgtag ctagtgccat tctctgatgt ttttaaatta ttatacttgc attatacttt
     5581 gccacaacaa ctcttgtatt tctttccgct accacaagga catagatcat tacgacccac
     5641 tttcttctca acacgcactg gttctttgtg tattttttct cgggtatctt gttgaccagc
     5701 attcattata tccgattttt gcgtattgta tttactaaaa tcttgtcgtc gctcttcttt
     5761 tgcctctctt aatgtttctc cttctttcac cggaatttga ccacgcatca aaatagcaat
     5821 cgctttgtaa tttatagcat caatcatttt cttaaatagc ccaaatgact ctagcttgta
     5881 gattaataat ggatcttttt gctcataact agcattttgt actgagttac gtaattcatc
     5941 taattcgcgt aaatgctctt tccaagcttc atctattgta tgcaacaaaa tagatttttg
     6001 gaacgacttc acaatttctt ttgcttgtgt ttcgtatgcc aatttaagat taacaggaat
     6061 attatacaac ttctttccat cagttaatgg aattaaaata ttctcatata ttttcccttg
     6121 ttcttcgtat acacgtttga tgacaggata ggcgatttcg gcaagtttat cggctcttcg
     6181 cgaaaaatta gccatcacca aagaaaatac tttttctact aaatcaattg tttttgtgcg
     6241 tttaatttcg ccttcctcca attggatatc taccccaaac agtttcaaac aatccaaacg
     6301 aaactcttct tcatcttgta tatcatcatg ttgatttacc aagttttcta caccatcgta
     6361 aatcatattc acaatatcaa tcccaatacg ttcgccaata agtgcatgtc tgcgacgagc
     6421 ataaatcact tcacgttgcg aattcatgac atcatcatat tctaacaaac gcttacgaat
     6481 accaaagtta ttttcttcga ccttcttttg agcacgttca attgaattag atatctgacg
     6541 atgttccatc atttcatttt cttgcaagcc taatctatcc ataatcccca ctatacggtc
     6601 ggatccaaat aaacgcatca acgaatcttc aaaagacaca aaaaatactg aagaacctgg
     6661 atctccttga cgaccagaac gaccacgtaa ctgacggtcg atacgacggc tttcatgacg
     6721 ttcggtacca ataattgcca aacctcccgc agcttttgat tcaggagtta atttaatgtc
     6781 agtaccccga cccgccatat tggtggcaat ggtaactgtt ccacttttcc cagcttgagc
     6841 aacaatatcc gcctcttttt gatgcaattt agcatttaat acattgtgtg gtattttgcg
     6901 aagtttcagc atcttactta ccagctcgga tatttctaca gaagtagtac ccactaacac
     6961 aggacgacct tgtgctacca atttttctac ttcctctata actgcattgt atttggcacg
     7021 agcggtttta tacactcgat cgttcatatc tacacgagcg ataggacgat tggttggaat
     7081 cacgacaaca tctaacttat aaatatccca aaactcacca gcctccgttt ctgctgtacc
     7141 agtcatacct gctaacttgt tgtacatgcg aaaataattt tgcaaagtaa tggttgcata
     7201 ggtctgagta gcagcttcca ccttcacacg ttctttggct tctatagctt ggtgtaaacc
     7261 atcagaataa cgtctaccgt ccataatacg acctgtttgt tcgtcaacaa tcattacctt
     7321 gttgttcata acaacatatt ggtcgtcttt ttcaaataaa gtatatgctt tgagcaattg
     7381 attaattgta tgtacacgtt ccgattttat cgagaactct tgcatcaaca cgtctttctt
     7441 agcttgcttt tcttcatcag acagttttgc cttttcgatt tcagccattt cagagccaat
     7501 atctggaagc acaaaaaata atgcatcgtc gctatcgcca gtaattaaat caataccttt
     7561 atcagtcaat tcaattgtat tgtgctgctc gttgataaca aaatacaaat cgtctgtaac
     7621 ctcgtgcata cgacggttat tttgctccat gtaaatagcc tctgttttca gcatagatgc
     7681 tttcactcct ggctcactta aatatttaat cagtgcctta ttttttggca accctttaaa
     7741 agctctgaat agtaacaaag agcctttttc ttgctctttc gcatctgcag attgcattaa
     7801 tcgttttgct tcaacaaatg ttttagtaac taggtttttt tgctcagcta caatacgttc
     7861 tacttttgag cagtattgct cgtataattg ctcttcccct tttgcaatag gacctgaaat
     7921 gataagaggt gtacgagcat catcaatcag tacagagtcc acctcatcta caatggagta
     7981 gttatgtttt cgttgtacta aatcctcggg agtagatgcc atattatccc gtaagtaatc
     8041 gaagccaaac tcgttgttgg taccaaaggt aatatcagcc aaatacgctt ttcgtctgtc
     8101 atccgaattg ggacgatgtt tatcgataca atctacacgt aatccatgaa acatataaat
     8161 gggtcccatc cattctgagt cacgtttaga taaatagtcg tttacagtta caacatgtac
     8221 accattacca ctcaaggcat ttaaaaaaac aggcaaagta gctaccaatg ttttaccctc
     8281 ccctgtggtc atctcggcta ttttaccttt atgcaataca acgccaccta taagttgcac
     8341 atcatagtgc accatatccc atttaatgac atttccacca gcttcccatt catttttgta
     8401 aatggcttta ttactcacaa tatccacaaa atcatgtgtt actgcaagtt cacggtcaaa
     8461 atcagtggct tccacctcta ttgtttcatt ttctttaaaa cgtcttgcag tatcttttac
     8521 aatagcaaac acttcgggca aggcgttatc aagagcctct tctaatttag ttattatggc
     8581 tttttcgata gcatctattt cattgtacaa tttatctcga tgttcaatag aagtagcatc
     8641 aatagattgt tttaacgtag cgatacgttc acgctcatcc gaaacagttt gttgaatttc
     8701 aagtcgtacg ccttgcagcc tattacgcaa ttcgtcgttt gaaagttgct ctacttgagg
     8761 atagattgcc tttatttttt caataaatgg cattatctct tttaaatcac gctgtgactt
     8821 tgaaccaaat aacttgctta aaatactatt taatcccata atggtaaatg ttgctaaatg
     8881 aaaaaatgtt gccttttaga tatacaaagg cacaaagata acaatattaa ttttcttata
     8941 tagaaactgt ttaagattaa ttaaagataa taaatcttaa acagatcgta tttcttgtta
     9001 tgaaatacgt aaaacgcgac ctactcgcaa tgtagtttta gtggtgatat tattcagtcg
     9061 acacaaattc cgtatagtag taccttgcct tctggcaatg gtagataagg tatcccctct
     9121 tcttacagta taatttctca tacgatccac gtccagctta taccgaaagg tttctgcact
     9181 aactaataat tctggcgttt taactgttaa attatcaaaa catatcaaag attcagggtt
     9241 aatcgcattt cccaaatacc gtacctcata atgtaaatga ggacccgtac ttcttcctgt
     9301 atttcctcct aaaccaatag gatcacccgc ttttactaac tgctctgtat caactaatat
     9361 tttgcttaaa tgaccataaa tagtttcgag tccattaaaa tgcctcacga gaacataata
     9421 tccataccct ctacctattt tggctatacg taccattcca tcaaatgaag aacgcacagt
     9481 atctccaaca ttcaatttta aatcaatacc atagtgataa cgagtacgac gcggaccaaa
     9541 atgtgatgtt atgcgttgta ccttatcaag tggatgagta tacccactaa catctacacg
     9601 gaaagtatct ttgctatgta ataaatttat attatacgga ttgaccgccg tattgctcca
     9661 gctgttgtaa ggtgcattaa aagatatttc atcaaaattt tcaaaatcat ccacatcagc
     9721 aaaatcatct tctgtatttt cgtttagcaa cataagactg ctagatgcat tcttcgggtc
     9781 ttctatgcga aatatattat cgtgcaaagt tacagccaac gcactatttg tgattgaaac
     9841 aacaaaacta aatacaataa gacgtaaaaa atgataactc atttttttat ttttataatt
     9901 cgtcattggc atataaaaag tctaaactat gtggtggaaa attatatatt tcttctggct
     9961 taaaaaagcg atttagttca attgcagcat tttcaacaga gtcggatgta tgaacaatgt
    10021 tttcttgtac actcatacta taatcgccac gtatggttcc tgaagcagca ttacgaccat
    10081 tggtaactcc cgccatattt cttaccacct gaacagcatc taatccagac caacaacata
    10141 cgataacggg tgttgtttgc atggcatttt taatacgttg gaaaaagggt ttttgggcta
    10201 aatgagcata atgctcggca agaatttcgt cagataattc tatcattttc atcccaataa
    10261 ggtgtaaacc tttcttttcg aagcgagata ttacttctcc aattaagcct cttgaaatag
    10321 ctgaaggttt aataataacc agtgtttttt ccataatttg attttacgac atacaaaaaa
    10381 acaatttatt ctttagttaa ccaaattttt tgcaaaaaaa attatagaat ttttacatat
    10441 caactaatta tactccaatt ttgttgttct cttttctttg ttttgagctc ttttactata
    10501 atttcgtttg ctggggactt cagttgcgga tcgttttcta gaatttctga agcacactct
    10561 ctggctagtt gaacaaaatg tccatcttta gctaaattgg caatatgtag ttgaaaaggc
    10621 aagccactct gttgggtacc ttccaaatct cctggtccac gaagctgtaa gtcagcctcg
    10681 gctatttcaa acccgtcgtt ggtacgggtc atgatttcta agcgtttgcg ggtttcgttg
    10741 gatatttttc gcgacgttac caatacacaa tacgattggt cggctcctct accaacacgc
    10801 cctctgagct gatggagctg tgccaatcca aatcgctctg cattttcgat taccattaca
    10861 ctggcgtttg gcacattcac ccccacttca atcacagtag tagcgaccat aatttgagat
    10921 ttatgttgca caaactcttg catagcagca tctttttcag ctggtttcat tttgccatgt
    10981 accatgctta cttgcaaatc tggaaaaatt gattgtatgt attgatagcc atctgtaagg
    11041 ttttttaaat ccatcttttc ggattcttct atcaatggat acacaatata tacttgcctg
    11101 ccaagtgaga gttgttgttt taaaaaacga ttaagagcct ctcgttttgt atcttcaaaa
    11161 atatggtttg ttactattgg ttttcgtcca gggggcaatt cgtcaattac ggaaacctcc
    11221 aaatcgccat atacagtcat agccaaggta cgtggaatgg gagtagcggt cattactaaa
    11281 acatgaggag gacgtgtgtt tttattccac aatttagctc gttgtgccac accaaatcga
    11341 tgttgttcat caattaccac tatgcctaaa ttcttaaaat taactgtatc ttctattaca
    11401 gcatgtgtgc caattaaaat atgtacagaa ccattcagta agccctcgtg aatatgttct
    11461 ctgtcttttt ttttcgttga acctgtaagt aattttactt gtatgggtaa tttgtttatt
    11521 tggctttcaa tagtttcaaa atgttgcctg gctaaaatct cagtaggagc cataatacat
    11581 gcctgataac cattatcaat agccataagc atacacatta gagctacaat agttttgcca
    11641 ctacctacat cgccttgcaa caacctgttc atttgataag ctccacgtac attattacgt
    11701 atctctttaa gtactctttt ttgagcattg gttagttcaa agggcaagca tttttcgtaa
    11761 taggcatgaa acatttctcc aatgtgatta aattgaaacc ctttaaaact agttttaaga
    11821 gcatgagcct gacgtaatat tgcaatttgt atataaaata gttcttcaaa ttttagtcgt
    11881 gcttgtgctt gttctaaaag aggcatattg ctggggaaat gaatattgat taatgcttcg
    11941 taaagtgaaa taaaacggta tttgtgaagg atactagttg gtaaagattc gggtaatgat
    12001 ggtgtaatgg tttgtaatgc ttggtaaatg agtttttgaa ttgcttttga attcaaaaac
    12061 ttattcttca ttttttctgt tgtatgatat agcggcataa aacctgtcat actgtcggga
    12121 gtaggttttt ctatatcagg atgcacaaaa ctgtagttgc cgttaaagaa attgggctta
    12181 ccgaaaacaa tgtaatcagt attcaagtga taggcatcca tcacatactt aataccctta
    12241 aaaaatatca gttctatgct gctgtgtccg tctgtaaaga tagcaatcag acgttgagcc
    12301 ctacccttgc cggcttcttt aaaaccaagt atttgcccct taatctgaat atatgctgct
    12361 gtcgaatcta tctcactgat ttgataaaac cgacttcggt ctatgtattt atacggaaaa
    12421 taatgcacca agtcgtccaa ggtatatatt tctaattcct tgttaagcaa ttcggcacgt
    12481 ttcggaccca tgccatgcaa atattttatg tcagactttg tcaaatccat tcttcaatat
    12541 tgcttcagca tgaattaaat cgataggcat agttatttta atattttcga tatttccatc
    12601 acacagatta attttgtatg ttgctgattc ataaacagat gcatcatcag tgaaacattc
    12661 cttatatggc tgctgatatg ctttcttcaa atttgtagaa ttaaatactt gcggagtctg
    12721 tacacaaaca tactcatttc tattaacaga caggctgctt ccattactaa tatggcgtaa
    12781 tgaatcaaca caatttataa ccggtagtgc agatcccttt tcttctgcca atgtaaatac
    12841 tcgattgatg caggtttgag acaccaaagg acgaactccg tcatgtatgg caactaatgc
    12901 accatcggga acaaattgaa gtgcattgta caccgaataa aaacgttgct cgcctcctgc
    12961 taccatttta taatcgggca tatctcgcaa atttttacac aaagattgcc aataggtcat
    13021 ctgtgtttca ggcaaaacca aaatgatatt tccccaattt gcaaatgctc gtaatgtacg
    13081 agctaaaatt ggttcaccat ttagaagcag aaattgttta ggaacattcg ctcccatcct
    13141 attacctgtt ccacccgcca caataatgat atatttttcc atagactttt gttattaaac
    13201 gcaaagttac atttttttct tagaatgtgt agtgtggaat cgtgtaatgt gtaactgttt
    13261 aatcggttaa ttgtttaatc gtttaatcgt gtataatcca atgttgaaat aatcaataat
    13321 gcgttgtagg ggcatgccat ggcgtgccct tcattttcga catgtgatta atataaatcc
    13381 aatgttgaaa tgatcaacat tggatttaca cactacacat tacacgatta cacacccctt
    13441 aacccttcac ccctaaccct tcacccctaa cccctaaccc ctcacccttt ttcaccctac
    13501 acaacagcct agtgaagggc gtggagtgtg gaatggatgg gaagattatt gattgcggtc
    13561 ttgcaaaaaa cgcttaatca taaaggataa aaaatacggg aaagtgtaga atttaccatt
    13621 aaatccgata ttcctattac acaactttat cccgaactta atgtccttga attttatatt
    13681 atcatcaagc aaattattaa gagatcttgt tgaattatca tttgccttta cctcaatagg
    13741 aataagtgaa tccctatctc taacaaaaaa atccatttct aaattagaac tttcattttt
    13801 ataaaaataa agctgatacc cttgtttaac aagcatatct ccaacaatat tttcgtaaat
    13861 agctcctttg taagtattaa agtttctatt tgctcgtaaa tcttcctgcg actcatcatc
    13921 taatgctgct ataagtaaac cggtatcttt gtaatacagt ttgtatgact tcggattatt
    13981 attaccctta agaggtagtt caacgctatc aagacaataa cagatattta taattcctgc
    14041 atcattaagc caatctacgg ttccaatata gtctctatta cgagcattct ttgcaatctt
    14101 ggtgatctga aattttttat tgtctttacc taaaaaaatt gatatgtgat tataaatact
    14161 tttgatcttt cccttatcta atccgatagc atatttagta atatcttctt cataatctaa
    14221 aagcaattgt ttttgtattc ccagcgtatc actaaagtta ttgtttttaa tataattgtt
    14281 tacaatggca ggcatgccgc cggtaatcat ataatctcta aaaatatctg acaaaacact
    14341 aacttcgaca gtacttaacg gcttaagttc tatcatttta ttgaataagc cttctatttg
    14401 ttgcgcacta tacgattttg cccataaaaa ctcctcaaaa tccattgagt gcatttcata
    14461 atcttcttta tatccaacac tgttagattc aatttcgtta tagtttattc ccattaaaga
    14521 accggaacaa ataacatcat aatttccatc taacgcaaat gattttaaac ttgttgcaca
    14581 tgcagaatat gcttgaattt cgtcgaaaaa aatgagtgtt ttcttttggg gcagtttaga
    14641 atcgggcaaa agaaacgaaa tatttttcag aatgctatca acgtcaaatc catcactaca
    14701 gatattttta aatttcggct gtaacgcaaa attaatagca ataacgcttt cgtaatttgc
    14761 ttcggcaaat gccattattg atgctgtttt gccaacttgc cgtgcgcctt ttactattag
    14821 cggcaaatga gcgttgttat gtttccactc tgcaaggaat gtatcaattt ttcgctttag
    14881 taggtgtgcc atatggtaac tgattgaata tgaatacaaa aatactcatt tttcacattt
    14941 ttccaaagtt ttacgccatt aatttcacat ttttcgacgc ttatatgtgt aatttttcac
    15001 atttttagac gcttttaagt gtgatttttc acatttttcg aaaatatggg ttaagggtgt
    15061 gtagtgtgta atcgtgtaat gtgtaaggtg aagggttagg ggttaagggt taagggtgtg
    15121 tagtgtgtaa tcgtgtaatg tgtaatcgtg taatgtgtag gggcgaattg cattcgccct
    15181 taaccattca cccttttatc aacccccacc ccaaatagtg caaaatcaaa acgaacgggg
    15241 tcggatgcat cgaatttacg taaactgcct gttaattctt ctactgcttt ccaatcgttc
    15301 tgagtgcgct gcaataggcc taattcgcgt cctgtgttgc cgctatgtac atctaatgga
    15361 atatataggc tcgacatggg tattcgtttc cataatccaa aatcaacgct tctatcatca
    15421 cgacgtacca tccaacgtaa atacatatta agtcttttgc aagctgaagc tttatctatt
    15481 gaagaaatgt gtttatcgca atgatcattg tgttctacac acataaattc agctctgaat
    15541 tttgacagta caatgcgaat atcattgctc tgcaaataat tttcttcaaa gaaatttcct
    15601 aggctgccgt atttaagata gaaatgtctg agtgacttaa taaaatcggc gaagtcccga
    15661 ccattaaatg ttctatgtac aaatgagtat aagtttttga tttctgcatc agatgcgttt
    15721 agaacaaaat cgtatggctt attatctaaa tatgtaagca tcctgtttgc actttttact
    15781 attgccttac gattgcccca tgcaatggct gctgctaaaa acccacttat ttctatatcc
    15841 tcttgcctat caaatttatg tggtatgctt ataggatcgt gctctataaa ttcctcacga
    15901 ttatatttat cataaagata ttctaaagta tctcttaaat tattatcgtc gtatattgcc
    15961 atgttattgc ctatttaata aagtgttata caaaaaccgc atgtgcctca taacgagata
    16021 catgcggttt tatgcgttaa gaataggact atttaattag taaactgtat gaataatctt
    16081 aatatcatca ctacggcctg attcatcaaa tatctcgaat tcgtaggttc ctaattcgta
    16141 accacttata tcaagcgcga attttgaagc tgcgggctca atatagttgt cttgaatata
    16201 ttctgaagaa ccttttttgt taaccaatat acgttggata cttgcatctg tttgtataaa
    16261 gaatgtagtc tgacttgcta agcgttttgt aaactcaaaa gcacgagcat cattgcatcc
    16321 tacaccaacc ttgtatgcaa acatacgagt tgcataaaaa gttcctgttc ctccggtaca
    16381 accacctgca ggtacaacct taatagcaaa tttcgcagag tcacctacag acaaatttcc
    16441 caatgtttca ccttccaaat ttatatacca ccaacctgtt ccacctgctt ctgccgtcat
    16501 ttgtaattca gattctattt gaccctcttc gaacatacgg caaagtttta cttggttatt
    16561 atcaatgctc tgagattgag tatatttata acgaaccaat acagtaggat cattttctgt
    16621 taaatcacta gttgtgatct caaactcgta aggcactgta aaatcgcaat tgtttttaag
    16681 tgacccgtca agagtatttt ttacttgact tttacccgaa catgttgtag gattagttgt
    16741 agcttttgta tctgtatctt cgtaatctac actactaacc gttgcaatta ctttaaggtc
    16801 gtaagttgtt gatgcgtcta aacccgtaat tgttgctgat gtagcatttg ctaaaacact
    16861 cggaccattt gaccaagttt ctgtaccact ttctttatat cttacagttt gagcggttgg
    16921 ggttccaccc gacaccgacc aattaagagt tacagaatta tatgtagaag ttttttctcc
    16981 aatagttact gtaacagatg aacatgttcc cgcataattg gctattgtac ctgtaggaaa
    17041 agtaacttct acgagtcctc cgcctgattt attaaaggca caataattgg cgtatgttaa
    17101 atcacctgct gtataagttg ttacaaaagt tgctgcccaa acatatccat ctacatcaac
    17161 tttagaaaat gacatttgta ccccattgcc cccattatca cgataataaa agttgcaatt
    17221 cgcaacactt gttaatacag aagtagaagt ataagtaact ttatatgtat tagtagctgt
    17281 tgtttcgtat gacatggtac cacccgttac agcataatcg caatatgttc ctgctcccaa
    17341 tgccgtagca ctaattaccg ccatggcggc ca
//
LOCUS       DAOSRP010000023        17308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3421_length_17308_cov_5.059004, whole genome shotgun sequence.
ACCESSION   DAOSRP010000023 DAOSRP010000000
VERSION     DAOSRP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17308
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_17308_cov_5.059004"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..689)
                     /locus_tag="PLJ82_02585"
     CDS             complement(234..689)
                     /locus_tag="PLJ82_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOR40428.1"
                     /translation="MEKLDSLDKEILKIIGKNARKPFIEVAELCGVSRAAVHQRVQKL
                     IDMKVIEGSGYHVNAQKVGYNTCTYIGIKLEKGSMYKDVVPELKKIPEIVECHFTTGP
                     YTMLVKLYAADNMHLMDLLNNRIQEIKGVTATETLISLEESIKKEVPIY"
     gene            complement(725..1600)
                     /locus_tag="PLJ82_02590"
     CDS             complement(725..1600)
                     /locus_tag="PLJ82_02590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR40429.1"
                     /translation="MKRTHVLAMLSVLLLAIFSVSCTNEKTVKIESDMDSLSYAFGIS
                     TTAGLMDYLGAQMGVDSTQMESFIKGVKDAAGEQSAEEKAYMVGLQIGSQIKDQMIPN
                     LNNAIFGEDSTKTINKDLFYKGFYEAMSAKAGMTVEDASKFTETKMEVIKNKMLEETF
                     GKNKVEGIEFLEKNKKEEGVQVTESGLQYKVITQGTSKRTPLAGEKVKVHYEGKLING
                     EVFDSSIKRGEPAEFPLNQVIPGWTEGLQLMTVGSKYILYIPYELAYGEKGAGQIIPP
                     FATLIFEVELLDVIK"
     gene            complement(1629..2339)
                     /locus_tag="PLJ82_02595"
     CDS             complement(1629..2339)
                     /locus_tag="PLJ82_02595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR40430.1"
                     /translation="MKYIVVALSVLVLSSCAVFAPKNKTKVEQKPALVLTNTIDSVSY
                     AIGVNVGTAFKQQMLQFPGGDYDVTILAEAFSVALKGDTTLLSYDQANAFLDAYLSKI
                     QEAETQKSKSEGETFLAENKKREGVVTLPSGLQYQIVKQGDGPKPKETDKVKVHYEGF
                     LLNGKKFDSSLDRGEPISFPLNQVIQGWTEGLQLMPVGSKYTLYIPYQLAYGEQGAGQ
                     FIPPFATLIFEVELLGIE"
     gene            complement(2339..2926)
                     /locus_tag="PLJ82_02600"
     CDS             complement(2339..2926)
                     /locus_tag="PLJ82_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR40431.1"
                     /translation="MEKVSYALGLSIGNNLIGSGITGVNLDKFVQGVQDVLEQKTAEI
                     SYDEAKIVLDEYFQDLANKMQALNKQIGAEFLAQNKQKEGVIELASGLQYQVLTAGTG
                     AKPSATDQVQCHYHGTMLDGQVFDSSVQRGEPAVFGVNQVIAGWVEALQLMQEGAKWR
                     LFIPYQLAYGERGAGQSIPPFATLIFDVELLKVIK"
     gene            complement(3148..4446)
                     /locus_tag="PLJ82_02605"
     CDS             complement(3148..4446)
                     /locus_tag="PLJ82_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOR40432.1"
                     /translation="MATQLKFETLQIHAGQEVDATTKSRAVPIYQTSSYVFDDAKDGA
                     DLFGLKKFGNIYTRLMNPTTDVFEKRIAALEGGVAALATSSGQSAQFLALNNILQAGD
                     NFVTTSHLYGGTYNQFKSQFKRLGVEVRFTENDEPATFEKYIDSNTKALYLETIGNPG
                     LNIPDFEAIAAVAKKYDIPLIVDNTFGAGGYLFRPLEHGASVVVESATKWIGGHGTSL
                     GGVIVDGGNFNWGNGKFPAFTEPSDSYHGLVFWDVFGANGPFGNIAFIIRARVEGLRD
                     WGNAISPFNSFLLLQGLETLSLRVERHVENALNLAQWLEKHPAVEYVNYPGLKSSKYH
                     SLANKYLKNGFGGVLSFKLKGDAEKADKLINNLKLISHLANVGDAKSLIIHPAATTHE
                     QLSPSEKLAASVFPGLLRISVGIEHLEDIKADIEQALKAL"
     gene            complement(4568..5515)
                     /gene="cysK"
                     /locus_tag="PLJ82_02610"
     CDS             complement(4568..5515)
                     /gene="cysK"
                     /locus_tag="PLJ82_02610"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOR40433.1"
                     /translation="MARIAKNLTELIGNTPLVQLSNYAEKRNLQANVIVKLELFNPAG
                     CVKERIALAMIEDAEADGKLKKGAEIIEPTSGNTGIGLAMVAAVKGYKLTLTMPETMS
                     IERRNLLKALGANLVLTPGALGMKGAIEKAAELHQANPFSYMPQQFDNPSNSSVHQRT
                     TGEEIWRDTNGKVDVFVGGVGTGGTISGVGAALKAHNPAIQIIAVEPADSPVISGGKP
                     GPHKIQGIGAGFIPKNYNPQVVDEVIQVSTDNAILTARQLAREEGMLVGISCGAAAYV
                     ATQVALRPENKGKNIVVLLPDTGERYLSTLLYAFEEYPL"
     gene            5658..6650
                     /locus_tag="PLJ82_02615"
     CDS             5658..6650
                     /locus_tag="PLJ82_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOR40434.1"
                     /translation="MKEKIPVTIVTGFLGAGKTSLLNMLIKQHPFTKFAIIENEFGEL
                     SVDGGLLATAKENIFELSNGCICCSLQSEFSTVIDRLLSSPYPFNHLLIETTGIANPA
                     TVIRPFLEGDKFPFLFELNAVVCLIDANNYKNKSNNFHEIHRQIAMSDTLIINKTDLI
                     TQEELNTLTKILHTKNPMATIFPNSYNSIDTRKILQAYSYREQQIASSIFSYTPNHTE
                     HKNDSQLIIQPTTLSHTVESVAFEYETAFDVDTFSSWIRMFVTLNQQTIFRIKGILCF
                     ANLPGKFILQVVGDIVTFESLPDDTEKNTCSKLVFIGTSIPKTDIEISLRNMLA"
     gene            complement(6711..7007)
                     /locus_tag="PLJ82_02620"
     CDS             complement(6711..7007)
                     /locus_tag="PLJ82_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40435.1"
                     /translation="MKKLLLYILLSFAVVGVYATTDKKPVNTVQETQQEVVSVVGDKV
                     YITAKAGEEINIFALTGQNVYRTLSEGGVLVVEDLPTSQILVVRIGAKAYKVKL"
     gene            complement(7088..7861)
                     /locus_tag="PLJ82_02625"
     CDS             complement(7088..7861)
                     /locus_tag="PLJ82_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOR40436.1"
                     /translation="MKILILGAGKMGTFFCDVLCTQHEVAIFDTNPKNLRFVFNTYRL
                     TQYDEIAEFAPELVINAVTLKHTIDAFQAVIAYLPSTCILSDIASVKTGLQEFYASTG
                     MRYVSTHPMFGPTFANLEDLSSQSAIIISESDHMGKAFFKDLYQSLKLQIFEYTFLEH
                     DETIAYSLSIPFASTIVFAAVMKHQDAPGTTFKRHLAIARGLFAEDDYLLSEILFNPF
                     TPSQVEKIRGELKFLLEIIEQKDSKAMHSYLQKLRENIQ"
     gene            complement(7882..8949)
                     /locus_tag="PLJ82_02630"
     CDS             complement(7882..8949)
                     /locus_tag="PLJ82_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOR40437.1"
                     /translation="MNDLQSILLPGIESKRPIVIAGPCSAETEEQVMDTAKQLAKNGI
                     KIYRAGIWKPRTKPGGFEGVGEIGLPWLQKVKEETGMYVSTEVATAKHVEAALKAGID
                     ILWVGARTSANPFAVQEIADSLKGVDIPVLIKNPVNPDLDLWIGAIERIYNAGIRKLG
                     AIHRGFSSYDKKIYRNLPQWHIPIELHRRLPNIPIICDPSHIGGRRELIASISQQAMD
                     LSFDGLIIESHICPDKAWSDASQQITPDILQFIINKLVIREGVQTTESLADLRLQIDN
                     IDNDLLEMLSKRMRISREIGTFKKEHNMPVLQANRYDEIMQKRVILGESMGMSPEFVT
                     KILEAIHEESVRQQIDVINSK"
     gene            complement(8963..10132)
                     /locus_tag="PLJ82_02635"
     CDS             complement(8963..10132)
                     /locus_tag="PLJ82_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR40438.1"
                     /translation="MMKILPANRLNEVSEYYFSRKLKEIAQMNAAGMNVISLGIGSPD
                     MPPSESTIETLAQVAKMPNTHGYQPTIGIPELRKAFADWYVRWYGVQLDANSEIQPLI
                     GSKEGILHVTLAFVNPGEKVLVPNPGYPTYTSLSKLLGAEVVQYDLCEDKGWEPDFEA
                     LQKMDLSNVKLMWTNYPNMPTGANASISLYQKIVDFAKKNAIVVVNDNPYSFILNNKP
                     LSILQIEGAKECCIEFNSLSKSHNMPGWRIGVIAANPTFIEWIVRVKSNIDSGTFRAI
                     QLAAVEALSNTNDWHKEMNIDVYQSRRDIAGKIMNALNCSYDDTQVGMFLWGKVPDNA
                     LSAEEITEKVLHEAKVFITPGFIFGSNGERYIRISLCATQAMLEEALNRIQLIIK"
     gene            complement(10137..10973)
                     /locus_tag="PLJ82_02640"
     CDS             complement(10137..10973)
                     /locus_tag="PLJ82_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOR40439.1"
                     /translation="MKTVAIQGVAGSFHDIAARNFFKEQEIKLVPCDSFKELFAVIKK
                     DNSIIGAVAIENTIAGSLLPNHTLLRESGFKIVGEYKLRIEHQLVAMPGQTIKDIEKV
                     YSHPIALMQCEDFLVEHPHLESVESEDTAASAKLIAEKKIMGRAAICSRLAAEIYGLE
                     VLAEGIETNKRNYTRFLIIADSWLADEMLKDIKADKSSLVFSLPHEEGSLAKVLSILS
                     YYHVNLTKIQSLPIIGREWEYMFYINLTFDNYMRYRQALDAIRPFTKDLQLLGEYKAF
                     EG"
     gene            complement(11286..12455)
                     /locus_tag="PLJ82_02645"
     CDS             complement(11286..12455)
                     /locus_tag="PLJ82_02645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR40440.1"
                     /translation="MKICLLTPRFPFPENGGDVLRINNIARYLKSQSHQLVLVSFYDN
                     EIEINDECKRLYDTIYTAPRSNIVSAINSLRFLMSGKPIQCGYYYSAQLLRIFKKVVE
                     TEKCDLYISHLLRMTPYLEKLNLQDKSIVEMTDALSITYQRSSEIKGISLKKIIYKFE
                     QEFIFRYEQKIVYTFKKVVLVSRNDIQYLSKKIKNNTSLSFHTNGVDCINTHLLSYQK
                     NKICFVGNMRTIQNQDAVKFFVTSILPLIMESVPDVRFYIIGAEPPTSIRNLADGKNI
                     FVTGFVESLVEFIQDSCVAVAPIRIAAGIQNKVLISMASGVPIVLSELISKAIPELTN
                     GTNCFIANDAMAFADACIRLITNSELRNQISTAGYNLVLNNYSWQACLANYEQMR"
     gene            complement(12455..13237)
                     /locus_tag="PLJ82_02650"
     CDS             complement(12455..13237)
                     /locus_tag="PLJ82_02650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40441.1"
                     /translation="MGRSGTAIPHIFTMISVCIATYNGSKYIKEQLQSILKQLGEEDE
                     IIISDDGSTDDTVNIIRGMSDNRIRVVNHTKDASYKNLSVASFLFASYNFENAIMHAR
                     GNIIYLSDQDDVWAPDRIEKTLPLMENNDLVMCNFSLIDENSTMVLEKYLPKDPAGRT
                     LFWNLWKIPFRGCCMTIKREVLLQSMPLPPQCVCHDIWIGTYLVHKGYKYTFINEPLH
                     YYRSHTTNVSPVVGKSPNSLWFKLAYRLRYLIQVFTHKSLVQ"
     gene            complement(13195..14214)
                     /locus_tag="PLJ82_02655"
     CDS             complement(13195..14214)
                     /locus_tag="PLJ82_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40442.1"
                     /translation="MIILTGTYPPEKCGVGDYSYQLLHTTVAKENKWQVYHARKNSIG
                     KFFATIKEINAMGDDSINLQYPSMGFLNSLMPHLFCLYFRIFSKKKISVTIHEYTQLG
                     WKGKFCTYIFLIFAHKLIFTNEFERHAAIRSWYKTAPKSTVIKIYSNIIAQDNLKKIE
                     NRQYDIGYFGYIRPEKGLEDYITVIANMKKKNTQLKTYILGQTQPVYKQYYEDVLKQA
                     TAVGIDIILNKDDVFVSNVLAETKIAYLPFPDGVSERRGSFLACVKNKMLVVTTEGLF
                     TTNAHRNLLDIATKDNAENVIQRLLSLSTYEWAEKQQQIEKFIDSEMPASWDEVAQQY
                     HTYLL"
     gene            complement(14211..15086)
                     /locus_tag="PLJ82_02660"
     CDS             complement(14211..15086)
                     /locus_tag="PLJ82_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR40443.1"
                     /translation="MISIIIVNYNTALFVNKAIESILLHTKDISYEIIIVDNNSSQDL
                     QSHISVFKEKVSVIYLAENIGFGRANNEGLKIATGRYVFFLNPDTILLNNAIKILADF
                     LDQHPHVGVCGGNLYDADGMPNHSYMPSLPSPIWELNSLFGNILFKLRYGKNIQFNFT
                     ANPLPVGYITGADMMVRGSVLEQCGAFDPDFFMYYEETELTYRIIKAGFSIMSVPNAK
                     IIHLEGQSFATNDNRESLKSISRKLYYKKTQPAIALLLSNILLRLNCLLRIVLFSILL
                     ESNKQKYWKLIYKNI"
     gene            complement(15083..16150)
                     /locus_tag="PLJ82_02665"
     CDS             complement(15083..16150)
                     /locus_tag="PLJ82_02665"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HOR40444.1"
                     /translation="MISFLLFFGLKGFVGRDVFLYYDLFSNLPTINHWNSDFFKLNDY
                     EVGFKMLMSVSKTIVPNFFFFAFLSVFIDAMILHFIIKRYSRYYVLAFIIYIVFYGYI
                     FEMDQIRNAKAVMLFLLSLRYVLNNKFLSYCLLNLIGFTFHSSSILFLFIYPFLKKRF
                     SLTFYAVVFIIGIVLFVLQIEYIKPMVMYVASKMGGVFQTKAEIYIASPIYGKDMFGI
                     TLGFFERIVSYCLFCFIYYKKLIDRDKKNLLFINLYVLFFIFYFYFAEFMVLAVRTSA
                     LFIAAYFVLYPNVLFEINRKLTRQLVVIMLTIFMFFRMATYVKYIEYKYENILFDTSS
                     YKIRKSYADESANYLQELDKK"
     gene            complement(16492..17124)
                     /locus_tag="PLJ82_02670"
     CDS             complement(16492..17124)
                     /locus_tag="PLJ82_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOR40445.1"
                     /translation="MKAIIKILITRLGILFSAKPLIILRNGMKLIFAVFYSGYIKGQL
                     KSVDSGFFVHYPAYVFNGSGIVIGSNFRAGRRLRIETFPVYHGQIFSPNLTIGNGVDI
                     QDDCHIGCINSISIGDNVLIASHVYISDHFHGEINAAELSTPPAFRGLHSKGAVVIKD
                     NVWIGEGVVILPNVTIGENCVVGANSVVTKSFSANNVIAGNPAKIIKTLL"
     gene            complement(17121..>17308)
                     /locus_tag="PLJ82_02675"
     CDS             complement(17121..>17308)
                     /locus_tag="PLJ82_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40446.1"
                     /translation="KEKQFTIFVFIAGIINILTSFALSRPLGDLGAALAVTLSEVVLC
                     ILVVHTFVKIKNNKSVQ"
BASE COUNT         5626 a         3264 c         2487 g         5931 t
ORIGIN      
        1 atactcaaaa catccgactt ccctgaaggg ttcttcggaa aatgtaggga ataaaaaaaa
       61 ttatatgtat aaaataatga gtggaaacaa tcaatatatt tccactcatt gtatattctc
      121 tttatacatc ttatatgttt ttcaatgtat tcttaaaaaa aggaataatg ccaaaataaa
      181 aaaagtagct attttacaaa atagctactt aacaatacat tgaaatataa accctaataa
      241 attggtactt ctttttttat gctttcttct aatgaaataa gagtttcagt tgcagtaact
      301 ccttttattt cttgaattct attatttaat aaatccatta aatgcatatt atctgccgca
      361 tacaatttta ctaacatagt atacggtcca gtagtaaaat gacattcgac aatttcaggt
      421 attttcttca gctctggcac cacatcttta tacatagagc ctttctccaa cttaatacca
      481 atatacgtac atgtattata accaaccttt tgagcattta catgataacc agacccttca
      541 attaccttca tatcaattag cttttgtaca cgttgatgta cagcagcacg cgatacacca
      601 cacaattctg ctacttctat aaatggctta cgggcatttt taccaataat ttttagtatc
      661 tctttatcaa ggctatcgag tttttccatg tcattgtatt aagattgcga agcaaaaata
      721 cgtattattt tataacatcc aataattcta cttcaaatat caatgtagca aaaggaggaa
      781 taatctgtcc tgcacctttt tctccgtatg ccagttcata aggaatatat aaaatatatt
      841 tagatcctac agtcattaat tgtaagccct ctgtccatcc aggaatcact tgatttagtg
      901 gaaattccgc tggttcgcca cgtttaatgg aactatcaaa tacttcgcca ttaatcaatt
      961 ttccttcata gtgcactttt actttttcac ctgctaaagg agttctttta gatgttcctt
     1021 gtgtaataac tttatattgt aaacccgatt cagtaacttg tactccttct tctttcttat
     1081 ttttctcaag aaactctatc ccctctactt tattttttcc aaaagtttct tccagcattt
     1141 tgttttttat tacctccatt ttagtttcgg taaacttcga agcatcttct actgtcatgc
     1201 ctgcttttgc agacattgcc tcatagaaac ccttgtaaaa taaatctttg ttaattgttt
     1261 tggtagagtc ttcaccaaaa atagcattat ttaaattagg aatcatttga tccttaattt
     1321 gactaccaat ctgcaaacca accatataag ctttttcttc agcactttgc tctcctgctg
     1381 catctttaac acccttaatg aaagattcca tctgagtaga atctactccc atttgtgccc
     1441 ctaaataatc catcaatcca gcagtggtag aaataccaaa ggcatacgat aatgaatcca
     1501 tatcactttc aatttttaca gttttttcat tcgtacagga aactgaaaaa atagccaaca
     1561 acaatactga tagcattgct aaaacatgtg ttcttttcat acaatttttt tctattattt
     1621 ataattaatt attctattcc taataattct acttcaaata ttaaggttgc aaaaggtgga
     1681 ataaattgcc ctgctccttg ttctccatat gctaattgat aaggaatata caaggtatat
     1741 ttagatccaa caggcattaa ttgcaaacct tctgtccaac cttgtattac ttgatttaaa
     1801 gggaaactga ttggttctcc cctatccaat gagctatcga attttttacc attcaataaa
     1861 aatccttcgt aatgtacttt tactttatct gtttctttag gttttggacc atcaccttgt
     1921 tttacaattt gatactgtaa tccacttggc aaggttacaa ctccttctct ttttttattt
     1981 tctgctaaga acgtttctcc ttcacttttc gacttttgtg tttctgcttc ttgtattttt
     2041 gataaatagg catctaaaaa tgcatttgcc tgatcgtatg acaataacgt tgtatcgcct
     2101 tttaatgcta cagaaaatgc ttcagccaaa atagttacat cataatctcc tccaggaaac
     2161 tgcaacattt gttgcttaaa agctgtccct acattcaccc cgatagcata actaacagaa
     2221 tcaatagtat ttgtaagaac taaagcaggt ttttgctcta cttttgtttt attctttggt
     2281 gcaaaaacag cacacgaaga taagactaaa acggacaatg ctactacaat gtatttcatt
     2341 attttattac ttttaaaagt tcaacatcaa aaatcaacgt tgcaaaagga ggaatagatt
     2401 gaccagcacc tctttctcca taagccaatt gatatgggat aaacaaacgc catttagcac
     2461 cttcttgcat taattgcaaa gcttctaccc atccagcaat tacttgattc accccaaata
     2521 ctgcaggttc gccacgttgc acagagctat caaacacctg accatcaagc atagtgccat
     2581 gataatgaca ttgaacttga tcggtagccg atggttttgc tcctgtacct gctgttaaca
     2641 cttggtattg taatccactt gcaagttcta ttacaccttc tttttgttta ttttgtgcta
     2701 aaaattcagc accaatttgc ttatttaatg cctgcatttt atttgctaaa tcttgaaaat
     2761 actcatcaag cacaatttta gcttcatcat acgatatttc agctgttttt tgctctaata
     2821 cgtcttgtac accttgtaca aatttatcta ggttcactcc cgtaatacca ctacctatta
     2881 ggttgttacc tatactcaaa cccaaggcat aacttacttt ttccatactt tttttctgat
     2941 tgctattttt tttaataaaa actctgcaaa gatacaattt cctaaacaag ttgacaacta
     3001 ttgaataaaa tataattgta ttttacaaat acttaacagt aaagtagtag catgtatgta
     3061 tttacaagaa tgcacaaccc tctactttta aaaacaataa aagctgtctt tgctcaagac
     3121 agcctcatta taacgcaaaa taatagctta tagtgctttt aaagcttgtt ctatatctgc
     3181 ttttatatct tcaagatgtt ctatgcccac agagatacgc aacaagcctg ggaaaacgct
     3241 tgcggcgagc ttttcggaag gtgaaagttg ttcgtgggta gtggcagctg gatgaataat
     3301 caatgatttg gcatcaccaa catttgctag atggcttatt agttttaaat tattaattaa
     3361 cttatctgct ttttctgcat ctccttttaa tttgaacgac aatacaccgc caaaaccatt
     3421 ttttaaatat ttgttagcca aggaatggta tttgctactt ttgagacctg gataatttac
     3481 gtattcaact gctggatgtt tttctaacca ttgtgctaag tttaacgcgt tctctacgtg
     3541 tctttctact ctcaacgaca gggtttctag cccttgcaat agcaaaaagg agttgaatgg
     3601 actaattgca tttccccaat ctcgaagccc ttctacacgt gctctgatga taaacgctat
     3661 gtttccaaat ggtccgtttg caccaaacac atcccaaaat accaatccat ggtaactatc
     3721 tgatggctct gtaaaagcag gaaacttacc atttccccag ttaaaattac ctccatcaac
     3781 aattacacca cctaacgaag tgccatgtcc gccaatccat ttagttgcac tttcgactac
     3841 tacggaagct ccatgttcta gcgggcgaaa caaataacca ccagctccaa atgtattatc
     3901 tactattaag gggatatcat attttttcgc tactgctgca attgcctcaa aatctggaat
     3961 attcaaacct ggattaccta ttgtttctaa atataaggct ttggtgttgc tgtcaatgta
     4021 tttctcaaat gtagctggtt cgtcgttttc ggtaaaacgc acttctactc ctaatcgttt
     4081 aaactgagac ttaaactgat tgtacgtacc tccatataaa tgagaagtag ttacaaaatt
     4141 atcgccagct tgtaagatat tatttaaagc caaaaattgg gctgactgac ccgaagatgt
     4201 tgctaaagct gctactcctc cttcgagggc tgctatacgt ttttcaaaca catcggtagt
     4261 aggattcatt aatcgggtat atatattccc aaattttttt aatccaaaca agtctgcacc
     4321 atctttagca tcgtcaaaaa catacgaact agtttgatat atgggtactg ctcttgactt
     4381 tgttgttgcg tctacctctt gacctgcatg tatctgtaat gtttcaaatt ttagttgtgt
     4441 tgccatcgtt ttatcttgta tttgtttttc tatatttgta ttttttactc gctgttttag
     4501 tatttttgct aatgcttgtt gaaaagcttc tgataaatta ctcatactat atgttgtttt
     4561 caatttctta taatggatac tcttcaaagg cgtataaaag tgtagacaaa taacgttcgc
     4621 cagtatctgg taataataca acaatatttt ttcctttatt ctctggacgc aaagccactt
     4681 gagtagctac ataagcagca gctccacacg aaatacctac taacatacct tcttctcgag
     4741 ccaattgtcg tgctgttaaa atagcattgt ctgtggatac ttgtatcact tcatcaacta
     4801 cttgagggtt ataatttttt ggaataaagc ctgctcctat gccttgtatt ttgtgcggac
     4861 caggtttacc acctgaaatt acaggagaat cagcaggttc aactgcaata atttgtatgg
     4921 ctggattatg tgctttaaga gcagctccta caccactgat tgtacctcca gtacctacac
     4981 cacctacaaa aacatctact ttcccattgg tatctctcca tatctcttcg ccagttgtac
     5041 gttgatgaac tgatgagtta gatgggttat caaattgctg tggcatatac gaaaatggat
     5101 tggcttgatg taattctgcc gctttttcaa tagctccttt cattcctaga gcccctggag
     5161 ttaataccaa atttgcaccc aatgctttta ataaattacg cctttcaatg ctcatagttt
     5221 cgggcatggt aagcgtaagt ttgtatcctt ttacagcagc taccatagct aatcctatgc
     5281 cagtatttcc actcgttggt tcaataattt cggctccttt ttttaatttt ccatcggctt
     5341 ctgcatcttc tatcatggct aaagcgatgc gttcttttac gcaacctgct gggttaaata
     5401 attcgagttt tacgattaca ttagcctgta aatttctttt ctcggcataa tttgataatt
     5461 gtactaatgg agtatttcca atgagttctg tcaggttttt tgctattcgt gccataccat
     5521 gtattttttt aatgtttaac tgatgcaaag ataactggta ataaatgcat acacaatagc
     5581 ttttgtgtgg gattttacat accacaagta tggtatatca agcaaaaata tgtattttcg
     5641 catgcttatt tatacgtatg aaagaaaaaa tacctgttac cattgttact ggatttttgg
     5701 gagctggcaa aacaagtttg ctaaacatgc ttatcaaaca acacccgttt accaaatttg
     5761 caattatcga aaacgaattt ggcgaactat ccgtggatgg aggtttatta gctactgcca
     5821 aagagaatat ctttgaattg agcaacggat gcatctgttg ttcgttacaa tcagaatttt
     5881 ctaccgttat tgatcgattg ctttctagtc cgtatccgtt taaccaccta ctcatcgaaa
     5941 caacaggcat agccaatcct gcaacggtaa taagaccttt tttagaagga gacaaatttc
     6001 catttttgtt tgaattaaac gcagtagtat gtttaattga tgcaaacaac tataagaata
     6061 aaagcaacaa ttttcatgaa attcatcgac aaatagccat gtcggatacc ctcatcatca
     6121 ataaaactga tttaattaca caagaagaac ttaatacgtt gacaaagatt ttgcatacaa
     6181 aaaatccaat ggcaactatc ttcccaaatt catataactc tatcgataca agaaaaatac
     6241 ttcaagccta ttcttacaga gaacaacaga tagcttcatc tatattttcg tacacaccaa
     6301 accatactga gcataaaaac gacagccaac tcattataca acctactacg ctctctcata
     6361 ctgtagaatc ggtagcattt gaatacgaaa cagcctttga cgtggataca tttagtagtt
     6421 ggattcggat gtttgttaca ctcaaccaac aaactatttt tagaataaaa gggattttgt
     6481 gttttgcaaa tttacctgga aagtttatac tacaggtggt gggggatatt gttacatttg
     6541 aatcattgcc agatgatacc gaaaaaaaca catgctcaaa acttgtattt ataggcacat
     6601 cgataccgaa aacggatatc gaaataagtt tacgcaacat gcttgcttaa caaaaaaata
     6661 ggagacacaa aattgcatct cccataaaac taacaacaaa tcttttatct ttataatttc
     6721 actttataag ctttggctcc aatacgtact actaaaattt ggcttgtagg taaatcttcg
     6781 accaccaaca ctcctccttc ggaaagagta cgatatacat tttgaccagt aagagcaaat
     6841 atgtttattt cttctcctgc cttagctgta atatatacct tatcacccac tactgaaaca
     6901 acttcttgtt gcgtttcttg caccgtattt acgggctttt tgtcggtagt agcatacacc
     6961 ccaaccaccg caaaagagag caatatgtag agcagtaatt ttttcatagg ttagtatatt
     7021 tctgcaaata tatatgattt tttgagtata tacaaatttt ttataaaaaa aatacttact
     7081 aattcattta ttgaatattt tctcgcaatt tctgcaaata tgaatgcatt gcttttgaat
     7141 ctttttgttc aataatttct agtaaaaact tcaattcgcc acgaattttc tctacttgcg
     7201 aaggcgtaaa agggttaaac aaaatttctg aaagcaaata atcatcttcg gcaaataatc
     7261 ctcttgcaat ggctaagtgt cttttgaacg ttgtacctgg agcatcttga tgtttcatta
     7321 ctgcagcaaa tacaatggta gatgcaaagg ggatagacaa cgaataggca atggtttcat
     7381 catgttctaa aaacgtatat tcaaaaattt gcaattttaa actttgatat aagtctttaa
     7441 aaaatgcttt tcccatgtga tcggattcgg aaataataat ggcactttgc gaactcaaat
     7501 cttccaaatt tgcaaatgtg ggtccaaaca ttggatgagt ggatacatat cgcataccag
     7561 tagatgcata aaactcctgc aaacctgttt ttacagacgc gatatccgac aaaatacacg
     7621 tacttggcaa ataagcaatt actgcttgaa atgcatcaat ggtatgtttg agtgttaccg
     7681 cattaataac tagttctggg gcaaattccg caatttcatc atattgtgta aggcggtagg
     7741 tattaaatac aaaacgtaaa tttttaggat tagtatcaaa aatagcaact tcgtgttggg
     7801 tacataatac atcacaaaag aatgtaccca ttttacctgc acctaatatg agaattttca
     7861 tatacttatg gatttattaa attacttaga gttaatcaca tctatttgtt gacgcactga
     7921 ttcttcgtgt attgcttcca atattttagt tacaaattcg ggggacatac ccattgactc
     7981 accaagaatt acacgttttt gcataatctc atcgtaacga ttggcttgta gtacaggcat
     8041 gttatgctct tttttaaatg tgcctatctc acgcgatata cgcatacgtt tagataacat
     8101 ttctaataaa tcattatcaa tattgtctat ttgcaaacgt aaatcggcca aactttccgt
     8161 tgtttgaaca ccctctctaa ttaccaattt attgattata aattgtaaaa tatctggcgt
     8221 tatttgttgc gaagcatcgc tccatgcctt atcagggcaa atgtgtgatt caataattaa
     8281 accatcaaaa cttaaatcca tagcttgttg actaatcgat gcaatcaatt ctcttcttcc
     8341 tccaatatgg cttggatcgc aaataattgg tatattaggc aaacggcgat gtagttcgat
     8401 tggaatatgc cattgaggta aattacggta tattttttta tcatacgaac taaatcctct
     8461 gtggatagct cctaattttc taattcctgc attataaata cgttcaatag caccaatcca
     8521 taaatctaaa tctggattaa cggggttttt tatcaacaca ggtatatcca ctcctttcaa
     8581 tgaatcggca atttcttgta cagcaaaagg attagcagaa gttcgtgcac ctacccagag
     8641 tatatcaata ccagctttca atgcagcttc tacgtgtttg gcagtagcta cttcggtaga
     8701 tacatacata cctgtttctt cttttacttt ttgtaaccaa ggtaaaccaa tttctcctac
     8761 accttcaaat cctccaggtt ttgttctagg tttccatatt cctgctctgt atatttttat
     8821 cccatttttc gccaattgtt tggctgtatc cattacttgc tcttctgttt cggcactgca
     8881 aggtccggct attactatcg gtcgtttcga ctcaatacca ggtaataata ttgattgtaa
     8941 atcgttcatg tttttatatt ttttatttga ttattaattg aattctattt aatgcttctt
     9001 cgagcatggc ttgtgtagca cacaaggaaa tacgtatata tcgttcgcca ttgcttccaa
     9061 aaataaaccc aggagtaata aaaaccttgg cttcatgcaa tactttttct gtaatttctt
     9121 cagccgatag agcattatca ggcacttttc cccataaaaa cattcctact tgcgtatcat
     9181 cataactaca attaagagca ttcataattt ttccagcaat atctctacgc gactgatata
     9241 catcaatatt catttcttta tgccaatcgt ttgtattaga aagtgcttct actgcagcta
     9301 actgtatggc acgaaatgta ccagaatcta tattactttt cacccgtaca atccattcga
     9361 taaatgttgg atttgcagca atcacaccaa tacgccaacc aggcatatta tgacttttgc
     9421 tcaacgaatt aaattctata caacattctt ttgccccctc tatttgtaaa atacttagcg
     9481 gtttgttgtt taaaataaaa ctgtatggat tatcgtttac tactacaata gcattctttt
     9541 tggcaaaatc tacaattttc tggtataaac ttatacttgc attggctcct gttggcatat
     9601 ttggataatt tgtccacatg agttttacat ttgataagtc cattttttgt aatgcttcaa
     9661 aatcgggttc ccagccttta tcttcacata aatcgtattg tacaacctct gcacccaaca
     9721 atttactaag cgaagtatag gtgggataac cagggtttgg cactagtact ttttcgccag
     9781 gattaacaaa cgccaaagta acatgcaaaa taccttcttt tgatccaatt agcggttgta
     9841 tctcgctgtt tgcatctagt tgcactccat accaacgtac ataccaatca gcaaaggctt
     9901 ttcttaactc tgggatacct attgttggct ggtagccatg ggtgttgggc atttttgcta
     9961 cctgtgctaa ggtttcaatc gtgctttctg atggtggcat atcggggcta ccaattccca
    10021 aacttataac attcatgcct gctgcattca tttgggctat ttcttttaac ttacgtgaga
    10081 aatagtattc tgatacttca tttaatcgat tggctggtag tatcttcatc atatttttat
    10141 ccttcaaaag ctttatattc tcctaataac tgtagatctt tggtaaatgg acgaatagca
    10201 tctaatgctt gtcgataacg catgtaatta tcgaatgtta aattgatata aaacatgtat
    10261 tcccattctc tcccaataat aggcaatgac tgaattttgg ttaagttaac gtgataatac
    10321 gacaaaatgg atagaacttt agctaggcta ccttcctcat gaggcaatga aaaaactaag
    10381 gatgatttat ccgcttttat atccttgagc atttcatcag ctaaccacga atcggctata
    10441 attaaaaacc gagtataatt gcgtttgttt gtttcaatgc cttcagccaa cacttccaat
    10501 ccatatattt cagcggctaa acggctacaa atggctgctc tgcccataat ctttttttct
    10561 gctattaatt tagcacttgc cgccgtatct tcgctctcaa cagactctaa atgagggtgt
    10621 tctactaaaa aatcctcaca ctgcattaat gctataggat gcgaatatac tttttcaata
    10681 tcttttattg tttggcctgg catcgccacc aattgatgtt ctatacgtaa tttatattca
    10741 ccaacaatct taaagccact ttcgcgcaac aaggtatgat taggcaacaa acttcctgca
    10801 atggtatttt caattgccac tgctcctata attgaattat cttttttaat aaccgcaaat
    10861 aattctttaa acgaatcgca tggcactaac tttatttctt gttctttaaa aaaattgcgg
    10921 gctgcaatat cgtgaaaaga acctgcgaca ccttgtatag caactgtttt catgttttta
    10981 gtttatattt cgttatctat atagcttaaa attaaaaaat cccatcaatt ttgacgggat
    11041 ttacttactt attatgttat tgtttacatt tttcatacca aaccccgttt tactttttgc
    11101 taaagtaaaa ataaaagtaa aagtatgtaa aagtaaaatt tctcatttca atattattct
    11161 ggcacaaaaa tacagttttt ttttaacaaa caatagaagt aaacctattt ttttcaaaat
    11221 tccatattaa ttgatcaatc atctaacata aattttacaa aatgtccaaa ttgtatcaat
    11281 tcagtttatc tcatttgttc gtaattagcc aaacatgctt gccatgaata attattcaaa
    11341 actagattgt atccagcagt tgaaatctga ttacgtaact ctgaatttgt tattagccta
    11401 atacatgcat ctgcaaacgc catagcatca tttgcaataa aacagttagt tccattagtt
    11461 aattctggta tagctttgga tatcaattct gacaaaacaa ttggtacacc actagccatt
    11521 gaaatcaaaa ctttattttg aatacccgca gcaatgcgta ttggagctac tgcaacacaa
    11581 gaatcttgta taaactcaac taaagattcg acaaatccag taacaaaaat atttttacca
    11641 tctgctaaat tgcgtatact tgttggaggt tcagccccta tgatataaaa acgcacatca
    11701 ggcactgatt ccattattaa aggcaaaata ctagttacaa aaaacttaac agcatcttga
    11761 ttttgtatgg ttctcatatt acctacaaaa catattttgt ttttttgata tgacaacaaa
    11821 tgggtattga tacaatctac cccattggta tgaaagctta aactcgtatt attttttatt
    11881 tttttactca aatactgaat atcgtttcga gaaactaaaa ctactttttt gaaagtatat
    11941 acaatttttt gttcatacct aaaaatgaat tcttgttcaa atttatatat tatctttttc
    12001 agtgaaattc cttttatttc agatgatcgt tgataggtta ttgataaagc atctgtcatt
    12061 tcgacgatag atttatcttg caaatttaat ttttctaaat aaggagtcat gcgtagcaag
    12121 tgagatatat ataaatcaca tttctcagtt tcaactactt ttttaaaaat tcttaataat
    12181 tgtgcagaat agtaataacc acattgtata ggcttaccac tcatcaagaa acgcaaagaa
    12241 ttaatcgctg acactatatt actacgagga gcagtataaa tagtatcata taatcttttg
    12301 cattcgtcat tgatttcgat ttcgttatca taaaacgata ctaacaccaa ctgatgtgat
    12361 tgcgatttga gataacgtgc gatattattg attcgtaaca catctcctcc attttcaggg
    12421 aatgggaaac ggggcgttaa caaacatatt ttcattattg aacaagggat ttatgagtaa
    12481 atacttgtat taaatatctt aaccgatatg ctagtttaaa ccataaacta ttaggacttt
    12541 ttccaactac aggtgataca ttcgtagtat gacttcgata gtaatgtaat ggttcattaa
    12601 taaaggtgta tttataccct ttgtgaacca aataagttcc aatccatata tcatgacaca
    12661 cacattgagg aggcaatggc atactttgta aaaggacttc acgtttgata gtcatacaac
    12721 agcctctaaa aggaattttc cataaattcc aaaataaagt tctcccagct ggatcttttg
    12781 gtaaatattt ttctaacacc attgtactat tctcgtcgat aagagagaaa ttacacatta
    12841 ccaaatcatt attttccatc aatggaagcg ttttttctat cctatccgga gcccacacat
    12901 catcttgatc tgaaagataa ataatattac ctcgggcatg cataattgca ttttcaaaat
    12961 tatatgacgc aaacaaaaat gatgcaacag ataggttttt gtaagaagca tctttcgtat
    13021 ggtttactac tctaattcta ttatcagaca ttcctctaat aatattaacg gtatcatcag
    13081 tacttccatc atcagaaata ataatttcat cctcctcacc caattgtttt aaaattgact
    13141 gcaattgttc tttgatatat tttgagccat tatatgtggc gatacatact gatatcatag
    13201 taaatatgtg tggtattgct gtgccacttc gtcccatgaa gcaggcattt ctgaatcaat
    13261 aaatttttct atttgttgtt gtttttcagc ccattcgtat gttgataacg acaataatct
    13321 ttgaataaca ttttcagcat tatctttggt tgcaatatct aataaattgc gatgtgcatt
    13381 cgtagtaaat aaaccttcgg ttgtaacaac taacatttta ttttttacac atgccaaaaa
    13441 agatcctcta cgctcagaga caccatctgg aaaaggtaaa taggctattt ttgtttctgc
    13501 caatacatta cttacaaata cgtcatcctt atttaatatt atgtctatac ctactgcggt
    13561 agcttgtttt aaaacgtcct cataatattg tttatacaca ggttgcgttt gtcccaatat
    13621 atatgtttta agctgagtat ttttcttctt catgttggct attactgtga tataatcttc
    13681 caatcctttc tctggacgaa tataaccaaa atatcctata tcgtattgtc tattttcaat
    13741 tttttttaga ttatcttggg caataatatt tgaatatatt tttattacgg tgcttttggg
    13801 ggcggtttta taccaactcc gaattgctgc atgacgttca aattcatttg taaaaatcaa
    13861 tttgtgtgca aatatcaaga aaatataggt acaaaactta cctttccatc ccaattgcgt
    13921 gtattcgtga atagtaaccg atattttttt ctttgaaaaa atacgaaaat atagacaaaa
    13981 taagtgaggc attaaagagt taagaaatcc cattgatgga tactgcaaat taatagaatc
    14041 atctcccata gcattgattt cttttatggt agcaaaaaac ttacctatag aattctttct
    14101 agcatgatac acttgccatt tattttcttt tgctactgta gtatgcaaca attgatagga
    14161 ataatctcct actccacatt tttcgggagg atatgttcca gttagtataa tcatatattt
    14221 ttatatatta atttccaata tttttgttta ttcgattcta acaagataga aaaaagaaca
    14281 attcgtaata agcaatttaa tcttaataag atatttgaaa gcaataaggc tattgcaggt
    14341 tgtgtttttt tataatataa ttttcttgaa atagatttta aactttctct attgtcattg
    14401 gtcgcaaaac tttgaccttc taaatgtatg atttttgcat tggggacaga cattatacta
    14461 aatccagctt tgattatacg atatgttaac tctgtctcct cgtaatacat aaaaaaatca
    14521 gggtcaaaag ccccacactg ctctaacaca ctccctctga ccatcatgtc tgctcctgta
    14581 atataaccaa caggtaaagg gttagcagta aaattaaatt gaatattttt tccatacctc
    14641 aacttaaaaa ggatatttcc aaacaaagaa tttaattccc atatgggaga tggtaacgaa
    14701 ggcatatatg aatgattggg cataccatct gcatcatata aattgccccc acaaacacct
    14761 acatgaggat gttgatctaa aaaatcagct aatatcttaa tcgcattatt gagtaatatc
    14821 gtatccggat ttagaaaaaa tacataacga ccagtcgcaa tttttaaccc ttcgttattt
    14881 gcccttccaa agccaatatt ttcagcaaga taaattactg aaactttttc tttaaaaaca
    14941 gaaatatgag attgtaaatc ttgagaagaa ttattatcta caataataat ttcgtatgat
    15001 atatcctttg tatgtagcaa tatggattca attgctttgt ttacaaataa agcggtatta
    15061 taatttacga tgattatgga tatcattttt tatctaattc ttgtaaataa ttagcagact
    15121 catcggcata cgattttctt attttgtatg atgatgtgtc aaaaagaata ttttcatatt
    15181 tatattctat atatttcacg taagtagcca tacgaaaaaa cataaatata gttaacataa
    15241 taactacaag ttgtctagtt agctttctgt ttatttcaaa taatacattc ggatacaata
    15301 caaagtaagc tgctataaaa agagcagatg ttcgtactgc aagaaccata aattcggcaa
    15361 aatagaaata gaaaataaaa aataaaacat acaaattaat gaataaaaga ttttttttat
    15421 ctctatcaat caatttttta tagtaaataa aacagaataa acaataagac actattcgct
    15481 caaaaaagcc aagtgtaatt ccaaacatat ctttcccata tatgggtgac gctatatata
    15541 tttctgcttt tgtttgaaaa actccaccca ttttggacgc tacatacatt accataggct
    15601 taatatattc tatttgcaaa acaaataaaa caattccaat aataaacaca actgcataaa
    15661 aggtgagtga aaaacgtttt tttaagaacg gatagataaa gaggaataat atagagcttg
    15721 aatgaaatgt aaatccaata agatttaata agcaatagga taaaaattta ttatttaaaa
    15781 catacctaag tgaaagcaaa aataacatca cagcttttgc atttctaatt tggtccatct
    15841 caaaaatata cccgtaaaaa acgatataaa taataaatgc taaaacataa tatcttgaat
    15901 atcttttaat aatgaaatgt aaaatcatcg catcaataaa aacagaaaga aaagcaaaaa
    15961 agaaaaaatt gggaacaatc gttttactta cagacataag cattttaaat ccaacttcat
    16021 aatcgttcaa cttaaaaaaa tctgaattcc aatgattaat tgttggtaag ttagaaaaaa
    16081 gatcgtaata caaaaacaca tcgcgaccaa caaacccttt tagtccaaag aaaagcaaaa
    16141 aagaaatcat tacgattagc cgaatacctg taattcccct attgtatttt aattgataaa
    16201 attcgagaaa catcaataaa ataagaaata gcagaaaaag taaatacggt atagtataag
    16261 ttaaactaag aacttccatt gtaaataaat aaatttgaca ttctttctac gcctatattt
    16321 agctatcata ctacccgagt aaacattgct tagctaccaa ttataaaact tgacaaagat
    16381 actataaaag gtagtaagaa aaaaaagaaa aaggagaaat aaccatattc aaaataaatc
    16441 tctagtctaa gatactactt ttaggttcag ttactccatt agtaataatg actataaaag
    16501 agtttttata atttttgcag gattacccgc aataacattg tttgcagaaa aactttttgt
    16561 aacgacagaa ttcgcaccga caacacaatt ctctccaatt gttacatttg gaagaattac
    16621 cactccctcg ccaatccata cattgtcttt tatcaccact gcacctttgc tatgcagtcc
    16681 acgaaatgct ggaggcgtac ttaattccgc agcgttaatt tcaccatgaa aatggtctga
    16741 aatataaaca tgactcgcta tcaacacatt gtctccaata ctaattgaat taatacatcc
    16801 tatatggcaa tcatcttgaa tatcaacgcc attaccaata gtcaaattag gagaaaatat
    16861 ctgtccatga tagaccggaa aggtttcgat acgcaatctt cgaccagccc taaaattcga
    16921 tccaattaca attccactac cattgaacac atacgcagga taatgcacaa aaaaacccga
    16981 atctaccgat ttcaattgtc cttttatata cccagaataa aagacagcaa aaatcaattt
    17041 cattccgtta cgtagtatta ttaatggttt agcggagaat agtataccca atctcgtaat
    17101 caatatttta attatagctt tcattgtaca gatttgttgt ttttaatttt cacaaaagtg
    17161 tgtacgacca aaatacacaa gacaacttca gacaaagtga ccgctaaggc tgcaccaagg
    17221 tcgcctaatg gtctagacaa agcaaaagat gtcaatatat taatgatacc agcaataaaa
    17281 acaaaaatgg taaattgctt ttctttac
//
LOCUS       DAOSRP010000024        17088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3460_length_17088_cov_5.572066, whole genome shotgun sequence.
ACCESSION   DAOSRP010000024 DAOSRP010000000
VERSION     DAOSRP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3460_length_17088_cov_5.572066"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLJ82_02680"
     CDS             complement(<1..663)
                     /locus_tag="PLJ82_02680"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOR40447.1"
                     /translation="MKYFTLLCLTFILIYGKVYAISFDALTHDFGTIYEEKGTVSTTF
                     SFKNKSNKLLVIERIVPACGCSNTHISSRNLAAGESGNIVVEFDPSERPGNFDKSIYV
                     YSNDSTVRLSIQGKVIPSKQTIAKMYPYRFQFSRADKNTLSFGSILHTQQSTQYVNFI
                     NDSDKPVKLTFLQLPPFISTDSLFYNLLPGNEQIIPFHISVLGHNVWGEKNEKLVVVE
                     NGK"
     gene            complement(833..1261)
                     /locus_tag="PLJ82_02685"
     CDS             complement(833..1261)
                     /locus_tag="PLJ82_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOR40448.1"
                     /translation="MKSKLFIIGVLVNLFIGFAGIAYAQQSANNSNSTSSEVSAMAFE
                     KTVHDFGTFNEADGKVETVFVFKNTSKENLVVSRVQASCGCTTPDWSKEPIAPGKKGF
                     VKASYNPAGRPGNFNKSVTVTSNQGTQVLYIKGQVVPKAN"
     gene            complement(1351..2871)
                     /gene="guaA"
                     /locus_tag="PLJ82_02690"
     CDS             complement(1351..2871)
                     /gene="guaA"
                     /locus_tag="PLJ82_02690"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOR40449.1"
                     /translation="MAEKILILDFGSQTTQLIARRVRELNTYCEIVPYNKFPVGDTSI
                     KGVILSGSPFSVYDQAAFKTDLSLFRGQLPVLGICYGAQFIAQQSGGKVQPANSREYG
                     RAKFSVCQSNNPLLKGIQEKSQVWMSHGDTITDIPANFSIIASTHDVEIAAYAVQGEQ
                     TWGVQFHPEVFHTTDGSKLLDNFLNICGCKKDWSAASFIETTVAELKAQLGDDKVVLG
                     LSGGVDSSVAAVLLNKAIGKNLTCIFVDHGLLRKNEFETVLDDYAHLGLNVIGVNVKD
                     RFYAKLAGVTDPEQKRKIIGKEFIEVFDEEAHKIQDVKWLAQGTIYPDCIESLSITGT
                     VIKSHHNVGGLPEKMNLKLCEPLRLLFKDEVRKVGNELGMMSHLIKRQPFPGPGLAVR
                     ILGDITAEKVRILQEADDIFIKGLREWNLYDQVWQAGVILLPVQSVGVMGDERTYENA
                     VALRAVTSTDAMTADWAHLPYEFLGKMSNEIINKVKGVNRVVYDISSKPPATIEWE"
     gene            2967..3926
                     /locus_tag="PLJ82_02695"
     CDS             2967..3926
                     /locus_tag="PLJ82_02695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR40450.1"
                     /translation="MIQKIKALLREVISFIKSDFDAVSYLYTLTFCGICIYVNYTYNL
                     YDTFLVPSYSSGWSMLYFSIFYMAAYFMVSIPVLIIRKQTAILTQPLYYVKAIFFIVS
                     FAVVVGNFTYQDLKIESFTYLENHFLIRLIFFFKANITTLPLLIIAKYLWDRKEKGLY
                     GLTISNKHIAAYMSLFLLLLPFLVWISFTPDFMRAYPIYRYWWYEGIFHWENWQHTII
                     FESVYASFYVITELVMRGALIIGMASILGRHAILPMVAMYCVLHFGKPIGETISSIFG
                     GYILGVLAYQTRHIWGGVIIHIAIALSMEVMGFIHYYLGIHKS"
     gene            complement(4060..5073)
                     /locus_tag="PLJ82_02700"
     CDS             complement(4060..5073)
                     /locus_tag="PLJ82_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40451.1"
                     /translation="MNLTDKKYANYALVQEMMETVKVVQKFNAQQTKNVASTIQSVGK
                     LFLTGEGSSRIFPAKNAIRKSLTKGLPVSIATDGSRQSAQYDLSKFAVFCASNSGRTK
                     EVVLLAKQLAAIGNVNRFALSANSGTLLEEVCTETFVLTCGWEQAVAATKSVVEQALF
                     YESILWHIEQTGTPNLADLAAKIEQALTFTIDSSIVQAAAKAPTIYFAGYNDGVAEEL
                     TLKTNEITRKKSDFLEGTYAVHGIEEVMEAQDVVFLIDPIEEEIQKFQEVLEQGVCLK
                     IIAIASRPTPFTTILVPDAGALSPYVYLCAGWNLLVEIGLALGIDLDKPERARKVGNE
                     LIV"
     gene            complement(5113..7317)
                     /locus_tag="PLJ82_02705"
     CDS             complement(5113..7317)
                     /locus_tag="PLJ82_02705"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOR40452.1"
                     /translation="MPKLTILPSNSIGTNFIPTEFLSDNQNQFTFRNQQVNKPEDYWR
                     KLKSEEIERLVKNNNTATDWSKVLVTDEFDTDQILNNRFFGLVRIGSVRDVVIKHHDL
                     QLPVGITNSIVVSCDIGDDVAIHNVGYLSYYIIGDRCIISNVNELNTCNHAKFGNGIV
                     KEGEPEKIRVWLELMNETGCRGVAPFDGMIAADAYMWARYRDDEALMKALTQITQNSF
                     DTRRGYYGTIGDECVVKNSFVIKDVKVGTHCYIKGANKLKNLTINSSIEEPTQIGEGV
                     ELVNGIIGYGCHIFYGCKAVRFVMGNNSNLKYGARLINSFLGDNSTISCCEVLNNLIF
                     PAHEQHHNNSFLVAALVMGQSNLAAGATIGSNHNSRANDNEVQAGRGFWPGLCTSIKH
                     SCRFASFVMLSKADYPAEMYIPFPFSLLNNNVSKDQLEVTPAFWWMYNMYALERNTWK
                     FKGRDGRKRKVQHIELEALAPDSVEEIFDALHLLELWVGKAFYRKQQQNLSQLTDEAM
                     QQKGKELLFSTAEAVNALEVLGEGMEKTKRKVLIIKAYQSYHAYRDMVYYYAVNTIFN
                     WMQHDTSRTFADMVVALQGNRETKWANFGGQLMREQDVDALRQDIGNGKLTTWNQIHQ
                     RYDSLWEQYPLQKQQHAYASLCSVLGVSNLTQKQWLEVLDKSLVIQEFVRDQVYLSRK
                     KDFDNPYRRMTFRNEAEMTAAIGTIEDNGFVKEVRQMTVKFNDLVKEIKQRG"
     gene            complement(7618..8022)
                     /locus_tag="PLJ82_02710"
     CDS             complement(7618..8022)
                     /locus_tag="PLJ82_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOR40453.1"
                     /translation="MNPIDILKTHQLKRTVCREGILSALLSANKALSEEEVKSKIDGN
                     FDRTTFYRSFKTLIEHNVLHKIVVDEGLVKYAITIVGKTPEHHHAHFVCKKCHTVSCL
                     DAFTFKVPNLPSGYQTEDSDLLIKGVCKMCTI"
     gene            8259..9536
                     /locus_tag="PLJ82_02715"
     CDS             8259..9536
                     /locus_tag="PLJ82_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40454.1"
                     /translation="MKKTVFLVASLIGCLFFAQAQDNVSTEKPLIPVVTLSKDVNVKF
                     GGFMRAEYYVDSKEMVGALDDLFGFFPEKPVYDANGKDINNVVRHNFSTQATRFNALF
                     TGPSVFNAKSSAFFEFDFTGGNSVNLRYRHAYGQLSWAKTDVLFGKSWNPLAAIHFPS
                     VLGLHTAIPFRPFGRGDQVRVTYRLTDKISILGAALIQTEHRSTLETSAATDVRANPI
                     PDMHLQLFHKTNNLMLGLLSEYKITRPATFTEGTLGKFVTNETVSSYAVGGVVDYKLN
                     LFNAKASVLYGQNLSELYIAGGYAVKTHDVATGKRTYTPSNAISSWINFTYGKKWIAG
                     VFGGYHKNLGFSDNILDTTANGALFGRWQNIDHIYRASTSLTYKIERWVFGAEVDYNV
                     AAYGEVDYGKKGKIKNATETSNIRGVLSATFIF"
     gene            9699..9911
                     /locus_tag="PLJ82_02720"
     CDS             9699..9911
                     /locus_tag="PLJ82_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOR40455.1"
                     /translation="MEKITNWIKKNRLYFVGSILGGIGGYIYWYFIGCDSGGCPITSS
                     PYNSVIWGAIMGSLLLSMFKKDKKDE"
     gene            9904..10143
                     /locus_tag="PLJ82_02725"
     CDS             9904..10143
                     /locus_tag="PLJ82_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40456.1"
                     /translation="MSKYLKNWNTVRIIRLLLGASLSIAAYFSGHYLFVGLGVWFMIQ
                     AFFNVSCCGTAGCDSETPSNSSDTVFKNEIKEYKK"
     gene            10140..10634
                     /gene="trxA"
                     /locus_tag="PLJ82_02730"
     CDS             10140..10634
                     /gene="trxA"
                     /locus_tag="PLJ82_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR40457.1"
                     /translation="MKQLTLLAISIGLLVTSCSEASNKQQKQPTPTEKKIQTTNNTLI
                     MKPIYLTKAEFLKKVVNYETNPNEWKYLGDKPAIIDFYASWCGPCKMIAPILDELAAE
                     YAGEIYIYKIDTEQEQELAAAFGIQSIPTLLLVPMNENPQIIRGALPKASFKEAIDKV
                     LLKK"
     gene            10640..11005
                     /locus_tag="PLJ82_02735"
     CDS             10640..11005
                     /locus_tag="PLJ82_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR40458.1"
                     /translation="MESLCYIRNIYRLISKFEEQLQQKHDLSLNEGMVLCELKNASYS
                     SGEIANLVSLTHSNTSKVIATLEKKQLVTRIIGDTDKRKMFFSITPQGTQKVNELLCE
                     ESEIQRIAKNIESDILQEK"
     gene            11170..11538
                     /locus_tag="PLJ82_02740"
     CDS             11170..11538
                     /locus_tag="PLJ82_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOR40459.1"
                     /translation="MKAIYTILLLLAANIFMTFAWYGHLKLKDFKWFSALPLIAVILF
                     SWGIALFEYFFQVPANKIGFRENGGPFTLLQLKVIQEVITLVVFVAFSSLFFKTETFR
                     WNHAIGFMFLVLAVYFIFKK"
     gene            11651..11989
                     /locus_tag="PLJ82_02745"
     CDS             11651..11989
                     /locus_tag="PLJ82_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOR40460.1"
                     /translation="MNSENIKSQMRKGYLEFCILLILEKKPAYASDILNELKAAKLIV
                     VEGTLYPLLTRLKNSGYLDYNWIESLQGPPRKYYELTENGKQFLSEIEEAWQEIKNVV
                     EHIKQQPNNE"
     gene            12043..13707
                     /locus_tag="PLJ82_02750"
     CDS             12043..13707
                     /locus_tag="PLJ82_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOR40461.1"
                     /translation="MKKTVTINLNGIVFHIDEDAYMLLQHYLEQLSKQFSAEDEKEIL
                     NDIEARIAELFTEKLQNGKNVVEKPDVERVMEVLGNPSQFADNDNDDTDNQHAENDKR
                     KRTNKRLYRDPENKILGGVAAGIAAYLEWNILLVRLLFVLFLFISVGWSFLLYLVIWI
                     IVPEATTTAQRLEMHGEDVTIENIKTQVKNAQEYVQSDEFKSNAKNIGSRIERILSPI
                     VKTVFIVVSSIVGFTLLLVSLSLLAAFVIALLHADILLAFLPFSVGSGYLAIIGIVSI
                     FLLIITPTIGLFVGAIRLLSNKTPRKGRSVFGWILFTVWVISLVALISVVVRGTLEYK
                     PSYTRSFAHYPAENRTLDGFHTILLHNGVHAEYTQDSTQQVQIMAPDYTLQNIHTEVN
                     DSILNIYFDGETNSIFTKNFAIHIQSPTIKRIEVEGGSSITTNTAIKTSNLQLKVADL
                     SQAQLLLDVEKNVNVISTGLSRVQLSGNTHHLDLNVSNASMANTIDLVSKTGTVNVQN
                     AAKADIQVRDSVWIQANDAARVCYRGNPIIKQQAYNAAVVVRYNEQ"
     gene            13987..16047
                     /gene="htpG"
                     /locus_tag="PLJ82_02755"
     CDS             13987..16047
                     /gene="htpG"
                     /locus_tag="PLJ82_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOR40462.1"
                     /translation="MKKGNIGVTTENIFPIIKKFLYSDHEIFLRELVSNAVDATQKLK
                     TLASVGEYKGELGDITVRVTRDEANKTITISDKGIGMTAEEIDKYINQIAFSGAEEFL
                     DKYKNDAAAIIGHFGLGFYSSFMVAKKVEIITKSYKENSQAVKWSCEGNPDYTLENCD
                     KAERGTDIVLHIDDENQDFLQQSKINELLTKYCKFLPIPIAFGKKTDWKDGKQVDTDE
                     DNIINDTQPAWVRKPADLKDEDYNKFYRDLYPMSEDPLFHIHLNVDYPFNLTGILYFP
                     KVKNTIDLQKNKIQLYCNQVFVTDSVEGIVPDFLTLLHGVIDSPDIPLNVSRSYLQSD
                     GNVKKISSHITKKVADRLQDLFKTDRALFEEKWESLKIFINYGMLTDDKFYERAEKFN
                     LLKNVDNTCYTAEEYKKLIETNQTDKKGDIIYLYATDKDDQFSYIEAAKNKGYDVLLM
                     DGQLDVHLIAKLEQKWEKSRFVRVDSDSVDNLIEKEDRKKTSLSEKQQQNLTTVFSTV
                     APKTEKANYIVAFDAMGANALPVMITQNEFMRRMKEMSAMQGGMMSFYGEMPDSYNLV
                     VNTDHALVKGIIKQQEESCGKEMGHFDKTIADIDTKLAEINKANEGKKEDELPQEVKD
                     EKAALRKEMDALREQQKAILANFADGNNVVKQLIDLALLANNMLKGEALSKFVKRSAD
                     MIQA"
     gene            16171..16244
                     /locus_tag="PLJ82_02760"
     tRNA            16171..16244
                     /locus_tag="PLJ82_02760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16205..16207,aa:Arg,seq:acg)
     gene            complement(16349..16786)
                     /locus_tag="PLJ82_02765"
     CDS             complement(16349..16786)
                     /locus_tag="PLJ82_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40463.1"
                     /translation="MKNLLYIVFITLMWCLSLHAYAVNWDKLLVSYETGDTTKITKKY
                     TIAISGVYAFVGDKKREVIVLKNDGNTWKPMQILKPTTKKRVEFGKKITIWNDVVIIE
                     APKESKNGTIYVYRIVNNRWRRVQKIKNALRRAMLLYTNDWHC"
BASE COUNT         5615 a         3185 c         2873 g         5415 t
ORIGIN      
        1 tttgccattt tcaactacaa ctaatttttc atttttttct ccccacacat tatgtcctag
       61 tactgagata tgaaaaggaa taatctgttc attaccaggt aatagattat aaaataatga
      121 atcagtagaa ataaatggag ggagttgtaa aaaagtcagt tttacaggtt tatctgagtc
      181 atttataaaa ttcacatatt gagtactttg ctgtgtgtga agaatagagc caaagctcaa
      241 ggtattttta tcagcacggc taaattgaaa acggtacgga tacattttgg ctatggtttg
      301 tttgcttggt attacctttc cctgtattga caaacgcaca gtagagtcat tactataaac
      361 gtaaatgctt ttatcaaaat ttcctggtct ttctgatgga tcaaactcaa caacaatatt
      421 accagactca ccagcagcta gatttctact actaatatga gtattactac aaccacatgc
      481 tggcacaatt ctttcaataa caagtagttt atttgactta tttttgaaag aaaatgttgt
      541 tgacacagtc cctttttctt cgtatattgt tccaaagtcg tgtgttagtg catcaaaaga
      601 aatagcatat actttgccat aaataaggat aaaagtcaaa caaagtagtg taaaatattt
      661 catatttatt ggttattatc tatgttatgc atatttaatg gaggcattgc ccattggata
      721 gtacagcaaa caaatagaac gtgataatat aggtacaaag gtacataaaa aaaatgaaaa
      781 cccacactgc attcaatgtg ggttaaacaa atttgaaaaa tattattgtt ccttagttgg
      841 cttttggcac aacttgtcct ttgatatata aaacttgtgt tccttgattg ctcgttacag
      901 ttacactttt gttaaaatta ccaggtcgac cagctggatt gtatgatgct ttaacaaaac
      961 ctttttttcc tggggcaata ggttcttttg accaatcggg agtagtacat ccacaagaag
     1021 cttgcactct actaactaca agattctctt tgcttgtatt tttaaataca aatacagttt
     1081 caactttacc atctgcctca ttaaaagttc caaaatcatg aacggtcttt tcaaacgcca
     1141 tcgccgacac ctcagatgag gttgaattag aattatttgc actttgttga gcataggcta
     1201 tacctgcaaa accaataaaa agattaacta aaacgccaat gataaaaagt ttcgatttca
     1261 tagttgtata ttttttgtta ttatttactg ccacaaagct atgtaaaaaa acaaagaaat
     1321 gttctttttt taattttttt taaacttata ttactcccat tcaatagttg cgggtggttt
     1381 ggagctaata tcatacacta ctctatttac tccttttact ttattgatga tttcgtttga
     1441 cattttgcct aaaaactcgt aaggcaagtg tgcccaatcg gctgtcatag catcagtaga
     1501 tgttacagca cgcaaggcta ccgcattttc gtaggtacgt tcatcaccca ttacaccaac
     1561 agattgcaca ggcaataaga taacaccagc ttgccatact tggtcgtaaa gattccattc
     1621 acgcaatcct ttgataaaaa tatcatcagc ttcttgtaat atacgtactt tttcggcagt
     1681 aatatcacct aatatacgta cagccaaacc aggacctgga aatggttgac gcttaataag
     1741 gtgcgacatc attcctagct cattacctac tttacgcacc tcatctttga aaagtaaacg
     1801 taatggttcg cacaatttca agttcatttt ctctggtaac ccacctacgt tatggtgcga
     1861 tttaataact gtgcctgtga tagaaagaga ctcgatacag tcaggataaa tagtgccttg
     1921 agccaaccat tttacgtctt gtattttatg agcttcttca tcaaatacct ctataaattc
     1981 tttaccaatt attttacgtt tttgctctgg atcagttaca ccagctaatt ttgcataaaa
     2041 acggtctttt acatttaccc caattacgtt gagacctaaa tgagcataat catctaatac
     2101 tgtttcgaat tcatttttac gcaacaaacc gtgatctaca aaaatacacg ttaaattttt
     2161 gccaatggct ttgtttaaca gtaccgctgc tacagaacta tcaacacctc ccgaaagacc
     2221 taaaactact ttatcatcac ccaattgtgc ttttaactct gccacagtag tttcgataaa
     2281 agaagcagct gaccaatctt ttttacatcc acagatgttt aaaaaattgt ctaacaattt
     2341 gcttccgtca gtagtatgaa acacctctgg gtggaattgc acaccccacg tttgctctcc
     2401 ctgtacggca taagcagcta tttctacatc gtgtgtactt gctataattg agaaattagc
     2461 aggtatatct gtaattgtat caccgtgcga catccatact tgcgattttt cttgaatacc
     2521 ttttaataac ggattatttg attgacagac agaaaacttt gctcttccgt actctctact
     2581 atttgcaggt tgcacttttc ctccagattg ttgtgcaata aactgggctc cataacaaat
     2641 acctaaaacg ggcaattgtc ctctaaaaag cgataaatct gttttaaatg cagcctggtc
     2701 atatactgaa aaaggacttc ctgacaaaat aacgcctttg atactggtat cgccaacagg
     2761 gaatttatta taaggcacaa tctcacaata tgtatttaat tcgcgcaccc tgcgtgcaat
     2821 caattgagtg gtttgtgaac caaaatcaag aataaggatt ttttctgcta tcataagttt
     2881 ttacaatttt gcccacaaaa gtagcaaata atttacactt ttttgtactt ttgacaaact
     2941 tttaaaatga attgacctta gcctgaatga tacaaaaaat taaagctctt ctacgtgaag
     3001 ttatttcttt tattaaatca gactttgacg ctgtgtcata tctttataca ttgacatttt
     3061 gtggtatctg tatttatgtt aattatacct ataatttgta tgacaccttt ttggtaccaa
     3121 gctattcttc gggttggtct atgctctatt ttagcatatt ttatatggct gcttacttta
     3181 tggtatccat tcctgtacta attatacgta aacaaactgc tattttaaca caacctttat
     3241 attacgtaaa agcaatcttt tttattgtaa gctttgctgt agtggtagga aatttcacct
     3301 atcaggattt aaaaattgaa agctttacat atttagaaaa ccattttcta attcgactaa
     3361 ttttcttctt caaagctaat attaccacac taccattgct tattattgct aaatatttat
     3421 gggatagaaa agaaaaagga ttgtatggat taaccatttc gaataaacac atagctgctt
     3481 atatgagttt attcttatta cttttgccat ttttggtctg gatttctttt acaccagatt
     3541 ttatgcgtgc atatcccatc tatcgttact ggtggtacga aggtatattt cattgggaga
     3601 actggcaaca taccataatc tttgaaagtg tgtatgcctc attttatgtc attaccgagt
     3661 tagttatgag aggtgcatta attattggta tggcaagtat tttaggtaga catgctatat
     3721 taccaatggt ggctatgtac tgtgtgttgc attttggaaa acctataggc gaaacaattt
     3781 cttcgatatt tggaggatat atattgggag ttttagctta ccaaacacga catatttggg
     3841 gtggcgtcat tatacatatt gctattgcat taagtatgga ggtaatgggc tttatacact
     3901 attatttggg aattcataaa tcataagtct ttttatatca tcagaataat taacaagctt
     3961 aaaccgattt tttgtttata gaataaagct taccctcaaa aaacaaaaaa atagaggtat
     4021 tacgtatcaa cataccccta tttctgtata taacatatct tacacaatca actcatttcc
     4081 tactttacga gcacgttctg gtttatccaa atcaatgcct aaagcaagac caatttctac
     4141 caataaattc caaccagcac acaaataaac ataaggagac aaggctcctg catctggcac
     4201 taaaatggta gtaaaaggtg taggtcttga tgcaattgca atgattttta agcatactcc
     4261 ttgttctaat acttcttgaa atttttgaat ttcctcttct attgggtcaa ttaaaaatac
     4321 cacatcttga gcctccatta cctcttcaat accgtgtaca gcataggtac cttcaaggaa
     4381 atctgatttt ttacgtgtaa tttcattggt ttttaatgtt agttcttcgg ctacaccatc
     4441 gttataacca gcaaaataga tagtaggtgc ttttgccgca gcttgcacaa tagatgaatc
     4501 aattgtaaac gttaatgctt gttcaatttt tgcagctaaa tcagctaagt ttggagtacc
     4561 tgtttgttcg atatgccaca aaattgactc ataaaacaag gcttgttcta ctacactttt
     4621 ggttgcagct acagcttgtt cccagccaca agtaagtaca aatgtttcgg tacaaacttc
     4681 ttctaacaat gttccgctat ttgcacttaa tgcaaaacgg ttgacattac caatagcagc
     4741 taattgtttt gccaacaata ccacctcttt ggttctgccc gaattagagg cacaaaacac
     4801 agcaaactta ctcaaatcat attgtgccga ctgacgagaa ccatcggtag ctatgcttac
     4861 tggcaatcct tttgttagag atttacgaat ggcatttttg gctggaaaaa ttctgctaga
     4921 accttctcct gttaaaaata atttgcctac tgattgaatg gttgatgcca cattttttgt
     4981 ttgttgggca ttaaattttt gtactacctt cactgtctcc atcatttctt gaaccagtgc
     5041 gtagtttgcg tactttttgt ctgttaaatt catttttttg cgttttttta acgtataaat
     5101 acctagagat gattaacctc tttgtttgat ttcttttact aaatcattaa attttactgt
     5161 catttgacgt acctccttta caaaaccatt gtcctcgata gttccaatag cagcggtcat
     5221 ttcggcttcg tttctaaatg tcattctgcg atatggatta tcaaaatcct ttttgcgcga
     5281 caagtacact tggtctctta caaactcttg tatcacaagc gatttgtcca atacttctag
     5341 ccattgcttt tgagttaggt tactcacgcc taacacagag cacaaggacg catacgcatg
     5401 ttgttgtttt tgtagtggat attgctccca caaactatca tatcgttggt gtatttgatt
     5461 ccaagttgtt agtttgccat tccctatatc ttgacgcaaa gcgtctacat cttgttctcg
     5521 catcaattga ccaccaaaat tagcccattt ggtttctcta ttcccttgta aagcaactac
     5581 catatcagca aaggtacgcg aagtatcgtg ttgcatccag ttaaaaatag tgtttactgc
     5641 ataataatac accatatccc tataggcatg atacgattga tatgctttga tgataagcac
     5701 ttttcgcttt gttttctcca ttccttcgcc taaaacctct aaggcattta cagcttcggc
     5761 agtcgaaaac aataattctt ttcctttttg ttgcattgcc tcatcggtaa gttggctaag
     5821 gttttgttgt tgcttacggt aaaacgcttt tcctacccac agttcaagta agtgcagggc
     5881 atcaaaaatc tcctctaccg aatcaggagc taaggcttct aattcgatgt gttgcacttt
     5941 tcttttacga ccatctctgc ctttaaattt ccatgtattg cgttctaggg catacatgtt
     6001 atacatccac caaaaagcag gagtaacttc taattggtct ttagaaacat tgttgttgag
     6061 caacgaaaat gggaaaggaa tatacatttc ggcggggtaa tcggctttgg ataacattac
     6121 gaaagaggca aaacgacaag aatgcttaat acttgtgcat aatccgggcc aaaaccccct
     6181 acccgcttgt acttcgttat cattagccct gctattgtga ttggaaccaa tggttgcccc
     6241 tgcagccaaa ttgctttgtc ccataaccaa agcagctacc aaaaaggagt tgttatgatg
     6301 ctgttcgtgt gctgggaata taagattatt gagcacttcg caacacgata ttgttgaatt
     6361 atctcctaag aacgaattaa ttagtcgggc tccatacttt aaatttgagt tatttcccat
     6421 aacaaaacgc accgctttgc atccataaaa aatatgacag ccatacccaa taataccgtt
     6481 tactagttcc accccttcgc caatttgtgt tggttcttca atagacgagt tgatggttag
     6541 atttttaagc ttatttgccc ctttaatata acaatgagtt cctactttta catctttaat
     6601 tacaaaagaa ttttttacta cgcactcatc gcctatggta ccataatagc cacgacgggt
     6661 atcaaagcta ttttgagtaa tctgcgttaa tgctttcatt aaagcctcat catctctgta
     6721 tcgtgcccac atataagcgt cggcagcaat cataccatca aagggagcta ctcctctgca
     6781 cccagtttcg ttcattaatt cgagccaaac acgtattttt tcgggttctc cttcttttac
     6841 tattccatta ccaaattttg catggttgca tgtatttagt tcgtttacat tagaaataat
     6901 gcacctatcg ccaataatat agtacgataa ataacctaca ttatgaatag ctacatcatc
     6961 gcctatatca cacgatacta cgatactatt ggtaatacct acgggcaact gtaaatcgtg
     7021 gtgttttatt accacgtcgc gtacagatcc aatacgaacc aacccaaaaa aacggttatt
     7081 taaaatttga tcggtatcaa actcatccgt aaccaacacc tttgaccagt cggttgcagt
     7141 attgttattt tttactaacc tttcgatttc ctccgatttt agttttctcc aataatcctc
     7201 aggtttatta acttgttgat tgcgaaaggt aaattgattt tgattgtctg ataaaaattc
     7261 tgttggaata aaattagtac caatagaatt cgaaggtaaa atcgttaact tgggcataaa
     7321 gtagaaattt gttatataaa atttattatc tcacataata cgaataagaa agcccgtaaa
     7381 actactaaaa atttgatata aatctagcat tttgtaaata aaattcgtat caaccatatt
     7441 tgctattctt ctgacttatg aaatattttt atggaaatac ctttttgggt cgaccatcct
     7501 tgtttatcgg taagagatat aaaaaaacaa agagatataa atagtttcat tcgatagaat
     7561 cctttttgat aagggtagtc atagtttact aaaaaccgat aatctgtttt tacaaaatta
     7621 tatagtacac attttgcaaa cgcccttaat gagtaaatca gaatcttctg tttgataacc
     7681 agacggcaaa ttgggaactt taaacgtaaa cgcatctaaa caactaacgg tatgacattt
     7741 cttacacaca aaatgagcat gatgatgctc aggagttttg ccaacaatgg taatggcata
     7801 tttcaccaag ccctcatcta ccacaatctt gtgcaacaca ttatgctcaa taagcgtttt
     7861 aaacgagcga taaaatgtag tccgatcaaa atttccatca attttgcttt ttacttcttc
     7921 ttccgacaat gctttgttgg ctgacaataa tgcagagaga attccttctc tacaaacggt
     7981 tcgtttaagc tggtgtgtct ttaaaatatc aattggattc ataaaataaa cttattttga
     8041 gtgaatagtt aattttgcaa caatgtttta tttgcatcat tgttgcaaat atgtaactaa
     8101 aaataatttt tccgatgtat actattaact atttttattt tattaccaaa tttgcataac
     8161 ttaaaacaaa tatttacttt tgcagcgaca aaatttacag ttaatcagtt ttttacacaa
     8221 ttcgataaca agataacaga taactaataa aaagtactat gaagaaaaca gtttttttag
     8281 tagcatcact cattggatgc ctattttttg ctcaggcaca agacaatgta agtacagaga
     8341 aaccactaat tcccgttgtt acattaagca aagatgtgaa cgtaaaattt ggcggtttta
     8401 tgcgtgctga atactatgtt gactccaaag aaatggtagg agcactcgat gatttatttg
     8461 gcttttttcc tgaaaaacct gtatatgatg caaatggaaa agatataaac aatgtagtac
     8521 gtcacaattt ctccacacaa gctacacgtt ttaatgcctt gtttacagga ccatcggtgt
     8581 ttaatgcaaa atcatcggca tttttcgaat ttgattttac cggaggaaat agcgttaacc
     8641 tacgctatcg tcatgcttat ggtcaattat catgggcaaa aacagatgtt ctatttggta
     8701 aatcgtggaa ccccttagca gccatccatt ttccatcagt attgggatta cacacagcca
     8761 ttccgtttcg tccgtttggt agaggcgacc aagtacgagt tacttacaga ctcacagaca
     8821 agattagcat tttaggtgct gccttaatac aaaccgaaca ccgtagcacc ttagaaactt
     8881 cggctgctac agatgtacgt gctaacccaa tacctgatat gcatttgcaa ttatttcata
     8941 aaacaaacaa cctgatgctg ggtttactgt ctgaatataa aattacacgt ccagctactt
     9001 ttaccgaagg cactcttggt aaatttgtta ccaacgaaac tgtatcttcg tatgcagtag
     9061 gtggtgtcgt ggactacaaa ctcaacttat tcaatgccaa agcaagtgtt ttgtatggtc
     9121 aaaacctaag cgaattatac atcgctggcg gatatgctgt aaaaacacac gatgtagcaa
     9181 ctggcaaacg tacctataca ccatcaaacg caatttcgtc atggataaac tttacgtatg
     9241 gtaaaaaatg gatagcaggt gtttttggag ggtaccacaa aaatttaggg ttctctgata
     9301 acattcttga tacaactgcc aatggtgctt tgtttggtcg ctggcaaaac attgaccata
     9361 tttaccgtgc atctacttcg cttacatata aaatagaacg ttgggttttt ggggctgagg
     9421 tggattacaa tgtggctgcc tacggagagg ttgattatgg caaaaaagga aaaataaaaa
     9481 atgctaccga aacatctaac ataagagggg ttttatcagc gacttttata ttttagaaag
     9541 tttattacac accatcatat aatcaaggtt acatactctt tttttaacct tgattatttt
     9601 ttttgttaaa ttttattttc ttttgatata ttttccattg gaaatttgtt tcattgtaaa
     9661 ataataccta tttttgcagt gtaaaaaaaa agctacacat ggaaaaaata acaaactgga
     9721 taaaaaaaaa taggctgtac ttcgttggga gcatactagg aggtattggt ggatatatct
     9781 actggtattt catcggatgc gattcaggcg gatgccctat aacctcatcg ccctacaaca
     9841 gtgtaatatg gggtgctatt atgggttcgc tcctattaag catgtttaaa aaagacaaaa
     9901 aagatgagta agtacctcaa aaactggaac acagttcgta taataagatt gcttttagga
     9961 gcttctttga gcattgccgc gtacttttcg ggtcattatc tttttgtggg attaggagta
    10021 tggtttatga ttcaagcatt ttttaatgta tcttgttgtg gtactgctgg ttgcgattcc
    10081 gaaacgcctt ctaatagttc tgatacagtt tttaaaaatg aaataaaaga atataaaaaa
    10141 tgaaacaact aaccttactt gccatatcca taggcttatt ggttacatct tgttccgaag
    10201 cctcgaacaa gcaacaaaaa caaccaacac caactgaaaa aaaaatacaa acaactaata
    10261 atacattaat tatgaaacca atctatttaa caaaagcaga gtttttaaaa aaagtggtta
    10321 attacgaaac caatcccaac gaatggaaat atttgggaga caaacccgca ataatcgact
    10381 tctatgcatc atggtgtggt ccatgtaaaa tgatagctcc catccttgat gaattggcag
    10441 cagaatatgc gggagaaatt tacatttata aaatagacac agaacaagaa caagaattgg
    10501 cagctgcatt tggcattcag tcaataccaa ccttgttgct tgtacctatg aacgaaaacc
    10561 cacaaattat tcgaggtgca ttacccaaag cgtcatttaa agaagccatt gacaaagtgt
    10621 tgcttaaaaa ataatcaaaa tggaaagtct gtgttacata cgaaatattt accgattgat
    10681 tagcaaattt gaagaacagt tgcaacaaaa acacgatctc agcctcaacg agggcatggt
    10741 attgtgcgaa ttgaaaaatg ccagctactc ttctggagaa atagccaatc tagtatcatt
    10801 aacacattct aatacatcca aggtaatcgc aacacttgaa aaaaaacaac tagtaacacg
    10861 cataataggt gatactgaca aaagaaaaat gtttttttca atcaccccac aagggacaca
    10921 aaaagtaaac gaacttcttt gtgaggaaag tgaaattcaa cgtattgcga aaaatattga
    10981 atcagatatc ttacaagaaa aataagaaga aatttgtttc acactctatc cttttttaca
    11041 ggcatattcg tgtcatgtaa aaaaggattt tttgtatcat aaaatgatgg ttgattatac
    11101 aaaaaataca aaaactttgt actacatttg cacaattttt agtgcatctt cttttaataa
    11161 aagaactata tgaaagccat ttataccatt cttttgttgc ttgctgccaa tatttttatg
    11221 acttttgctt ggtatggtca tttaaagttg aaagatttta aatggttttc ggcattgcct
    11281 cttattgctg ttatcttatt tagttgggga attgctcttt tcgagtattt ttttcaagtt
    11341 cctgccaata aaattggctt tcgcgaaaat ggaggaccct ttaccctgct ccaactcaaa
    11401 gtgattcaag aagtaattac cttagttgtt tttgttgctt tctctagctt attcttcaaa
    11461 accgaaacct ttcgctggaa ccacgccata ggctttatgt tcttggtatt ggctgtgtat
    11521 tttattttca aaaaataaat tgctttctat tttatttgtt aatatttctt aatttatagt
    11581 agtatgcatt acatagtact ataaacatac ttatctttgc actatcaact tacatacaaa
    11641 cgctatgtca atgaattcgg aaaatattaa atcgcaaatg cgaaaaggat acctagaatt
    11701 ttgcattcta cttatcctcg aaaaaaaacc tgcctacgca tcggacatac tcaacgaact
    11761 taaagccgct aaactaatag tagtagaggg cacattgtat cctttgctaa ctcgactcaa
    11821 aaacagtggg tatcttgact ataactggat agaatcgcta caaggtccac cccgtaaata
    11881 ctacgaactg acggaaaacg gcaaacaatt tttgtccgaa atagaagaag cttggcaaga
    11941 aataaaaaat gtagttgaac acatcaaaca acaacctaac aacgaataga cagaacactt
    12001 catcatcaat aacaatccct caaactacaa acatatactc ccatgaaaaa aacagtaaca
    12061 atcaacctca acggcatcgt ttttcatata gacgaagatg cgtacatgtt gcttcaacat
    12121 tacttagagc aattaagtaa acaatttagt gccgaagatg aaaaagaaat tctcaacgat
    12181 attgaggctc gtattgcaga attatttacc gaaaaattac aaaacggtaa aaatgtagtc
    12241 gaaaaacctg acgtagaaag ggtaatggaa gtgcttggca atcctagcca atttgctgac
    12301 aacgacaatg atgatactga caatcaacac gctgaaaacg ataaaagaaa aagaactaac
    12361 aaacgtttat accgtgatcc agaaaacaaa atactaggtg gtgtagcagc aggtatagct
    12421 gcctatttgg aatggaacat attgcttgta cgtttgctat ttgtgctatt tctatttatc
    12481 agtgttggtt ggtcattttt actatacctc gttatctgga ttatcgtgcc agaagcaact
    12541 actactgccc aacgactcga aatgcatggc gaagatgtaa cgattgaaaa cattaaaacg
    12601 caagttaaaa atgcccaaga gtatgttcaa tcagacgaat ttaaaagcaa tgccaaaaac
    12661 attggctctc gcattgaaag aatactctct cctattgtta aaacggtatt catagtcgtt
    12721 agttctattg ttggttttac cttgctactt gtatctttat cgttgctagc agcattcgtt
    12781 atagcattgc tacatgcaga tatattactt gcatttttac catttagtgt tgggtcgggg
    12841 tatttagcaa ttattggcat agtgagtatt ttcttactta ttattacgcc aactatcgga
    12901 ctatttgttg gagccattcg cttattatca aacaaaacac ctcgtaaagg tagaagtgta
    12961 ttcggttgga ttttgtttac agtatgggtc atcagtttag tagcactcat ctctgtagta
    13021 gtaagaggca cactggagta taaaccctcc tatacaaggt cttttgctca ttaccctgcc
    13081 gaaaatcgta ccctagatgg ctttcatacc atcttattgc acaatggcgt acatgctgag
    13141 tacacacaag actctacaca acaagtacaa attatggctc ccgattacac cttacaaaac
    13201 atccatacag aagtaaatga tagcatattg aacatctatt ttgatggaga aacaaattca
    13261 atctttacta aaaattttgc catacacata caatcgccaa ccataaaacg cattgaggta
    13321 gaaggaggct caagcattac gacaaacaca gccattaaaa caagtaattt gcaactgaaa
    13381 gtagctgatc taagtcaggc tcaactactg ctagatgtag aaaagaatgt aaacgtgatt
    13441 agtactggct tatcgcgggt acagctatcg ggcaatacgc atcaccttga cttaaacgta
    13501 agcaatgcca gcatggcaaa cacaattgat ttagtgagca aaacgggcac tgtcaatgta
    13561 caaaatgcag ccaaagcaga tatacaagta cgcgacagtg tgtggataca agccaacgat
    13621 gctgcacgtg tatgctatcg tggcaaccca atcattaaac aacaggcata caatgctgcg
    13681 gttgtagtac gctataacga acaatgacat gtatttgaac acaaaagagc ggtcaaaatt
    13741 tgaccgctct ttgtgatttt cataggtact cgacacaaaa tcctaggcta caagtatttc
    13801 gaatgtcaga cactacaaag atgatacttt tttttgaatt ctgaaatagc gtagtatatg
    13861 ttatacaata tgttttttag ttaaaattac gcatccacta tgcctttttg acagacagat
    13921 atacttggca ctacttttgc atagctaaag tgaaacaaat gtataactta aaataaatag
    13981 ttgagtatga aaaaaggaaa tattggtgta actacggaaa acattttccc tatcattaaa
    14041 aaatttcttt acagcgacca cgaaatcttt ttacgcgaac tagtttctaa tgctgttgat
    14101 gctacacaaa agctaaaaac attggcatca gttggcgagt acaaagggga attgggcgac
    14161 attactgtgc gtgttaccag agacgaagcc aacaaaacca ttaccatttc tgacaaaggt
    14221 attggtatga ctgccgaaga aattgataaa tatatcaacc aaattgcttt ttcgggagca
    14281 gaagaatttt tagataaata caaaaacgat gccgccgcta tcattgggca ttttggtttg
    14341 ggtttttact cttcttttat ggttgccaaa aaggtggaaa tcatcaccaa atcgtacaaa
    14401 gaaaatagcc aagcagtaaa atggagttgc gaaggtaacc ccgactacac attagaaaat
    14461 tgcgataaag cagaaagagg tacagatatt gtattgcaca ttgatgacga aaaccaagac
    14521 ttcttgcaac aaagcaaaat aaacgaattg ctaactaaat actgcaaatt tttacctatc
    14581 cccattgcct ttggcaaaaa aacggattgg aaagatggca aacaagtaga tacggacgaa
    14641 gataacatca taaacgacac acaaccagct tgggtacgca aaccagccga tttgaaagac
    14701 gaagattata acaaatttta ccgcgatttg tatcctatga gcgaagatcc gctctttcat
    14761 atacacctaa atgtggatta tccgtttaac ctaaccggta tattatactt tccaaaagta
    14821 aaaaatacca ttgatttgca aaaaaacaaa attcaattgt attgcaacca agtgtttgta
    14881 accgactcag tagaaggtat tgtgcccgat ttcttaaccc tattgcacgg agttatcgac
    14941 tctccagata ttccgctgaa tgtaagtcgc tcgtacttgc aaagcgatgg taatgtgaag
    15001 aaaatttcga gtcatattac caaaaaagta gctgacaggt tgcaagattt gtttaaaact
    15061 gaccgtgctc tatttgaaga aaaatgggaa agcctaaaaa tatttatcaa ttacggtatg
    15121 cttactgatg ataaatttta tgaaagagcc gaaaaattca atttactaaa aaatgtggat
    15181 aacacctgct atactgctga ggagtacaaa aaactgattg aaacaaacca aacagataaa
    15241 aaaggagaca ttatctactt gtatgcaacc gacaaggacg atcaatttag ctacatcgaa
    15301 gctgccaaaa acaaaggata cgatgtgtta ttgatggatg gtcagcttga tgtgcattta
    15361 attgctaaac tggaacaaaa atgggagaaa tcgcgttttg ttcgtgtaga tagcgatagc
    15421 gtggacaact tgattgagaa agaagaccgc aaaaaaacat cactgtcaga aaaacaacaa
    15481 caaaacctta caactgtatt tagcaccgta gcccccaaaa cagaaaaagc caactacata
    15541 gttgcttttg atgccatggg agccaatgca ctgcctgtga tgattacgca aaacgaattt
    15601 atgcgtagaa tgaaagaaat gtcggcaatg caaggtggca tgatgagttt ttatggcgaa
    15661 atgcccgact cgtacaattt ggttgtaaat accgaccatg cgctggtaaa aggtattatc
    15721 aaacaacaag aagaaagttg cggaaaagaa atgggacatt ttgacaagac cattgcggat
    15781 attgacacaa aacttgcgga aatcaataag gctaacgaag gtaaaaaaga agatgaatta
    15841 cctcaagaag taaaagatga gaaagccgct ttgcgtaaag aaatggatgc attgcgtgag
    15901 caacaaaaag cgattttagc caactttgcc gacggcaata atgtagtgaa acaactgatt
    15961 gacttggctt tacttgctaa taatatgcta aaaggcgaag ccttgagtaa gtttgtaaaa
    16021 cgcagtgccg acatgataca agcttaacac cgcttaaata aaggctgtgt ttgataaaaa
    16081 caaaagaaaa acacagcctt tttttttgaa ataaatttgc acaaaaacaa aaaacacctt
    16141 atctttgcat tcgctttttg aaagctacaa ggctgcgtag ctcaactgaa tagagcatct
    16201 gactacggat cagaaggtta taggtttgaa tcctatcgcg gtcacaaata aaagcacaaa
    16261 aaacacctac aaaagtactt gtaggtgttt ttttatttca atagatttac aacagaaata
    16321 gaggataaaa gcgtaaatat cggagatatt aacaatgcca atcattcgta tagagcaaca
    16381 ttgcacggcg tagggcgttt tttattttct gaactcttct ccatctatta ttcacaatac
    16441 gatacacata tatcgttcca tttttgcttt ccttaggagc ctcgatgata acgacatcat
    16501 tccagatcgt aatttttttg ccaaactcaa cgcgtttctt ggtagtaggt ttaagaattt
    16561 gcatcggttt ccaggtattt ccatcatttt ttagcacgat tacttcacgt tttttatctc
    16621 ctacaaaagc atatacccca ctaatagcaa tcgtatattt tttcgttatc tttgtggtat
    16681 ctccagtttc atacgacact aatagtttat cccaattaac agcatacgca tgtaaagaca
    16741 aacaccacat tagtgtaata aaaactatgt acaaaagatt tttcattatt ttgtatatag
    16801 caatttaaca ttcatagcat tcatctcagc tttcataaca aagtatacat ataatctatc
    16861 tagcataatc gtatcaatct ctagcatgta ttttaatcga tcgcaaagat acattatttt
    16921 tggcttaact cgtttaaagg aagacatcgt ttttcttgct cattcaatca ttaagttggg
    16981 aatgtaatcc gtttcatttt tagcaagaat ttctttttct tggaaaaact tgcgaatatt
    17041 tgcgaatata gacatttatt gaggaaatga taaaggggat gtgaaaaa
//
LOCUS       DAOSRP010000025        16784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3527_length_16784_cov_4.407974, whole genome shotgun sequence.
ACCESSION   DAOSRP010000025 DAOSRP010000000
VERSION     DAOSRP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16784
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3527_length_16784_cov_4.407974"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2521)
                     /gene="metH"
                     /locus_tag="PLJ82_02770"
     CDS             complement(<1..2521)
                     /gene="metH"
                     /locus_tag="PLJ82_02770"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOR40464.1"
                     /translation="MKTQKMTDIASILEQRILVLDGAMGTMIQEYRLQETDFRGERFQ
                     AHTHSQKGNNDLLTLSQPTIIETIYRKYVEAGADIISTNTFNSNRISMSDYGMENLVY
                     ELNYEAAKLAKSVTTHYTTSSKPLFVAGAIGPTNKTLSMSPKVEDAGYREISFEQLAT
                     VYAEQVEALMAGGVDVLLVETIFDTLNAKAAIYAIQDVFARLGKEIPIIISGTLLDKS
                     ARTLSGQTLEAFVISVSHAKPLAIGLNCSTGASDMLPHIIELGKLTDTFVSAYPNAGF
                     PNELGKYDETPQQMSNQIEEFLSHQAVNIIGGCCGTTPEHITRFAQLAASATPHQRTT
                     LEPFTRLSGLEPMVIKKENNFVNIGERTNVAGSKKFARLIADKKYEEAIQIARQQVEG
                     GAQIIDICMDDAMLDSAASMTDFLQLVATEPSVAKVPIMIDSSKWEVLEAGLRCVQGK
                     SIINSISLKEGEEVFLNHAKKIRQYGAAVVVMAFDEQGQADTFEKRIAVCQRAYTLLT
                     NIGFPAEDIIFDPNILAIATGLEEHANYAIDFIRTVEWIKKELPYAKVSGGVSNLSFS
                     FRGNNTVREAMHSVFLYYATQKGMDMGIVNPEMLEIYSNIPADLLELTEDVIFNRRAD
                     ATERLINYANEVKSNTSKETKTDEWRTKSLQERLGHAIIKGIYDYLDTDLTEARTQYA
                     KALEIIEGPLMDGMNIVGDLFGSGKMFLPQVVKSAQVMKKAVAFLLPYIEAEKDSNSH
                     NAGKIVMATVKGDVHDIGKNIVGVVLACNNFEVIDLGVMVPASEILRKAKEVNADIIG
                     LSGLITPSLEEMASVAEEMEKQGFTLPLLVGGATTSKLHTSV"
     gene            complement(3342..5735)
                     /locus_tag="PLJ82_02775"
     CDS             complement(3342..5735)
                     /locus_tag="PLJ82_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR40465.1"
                     /translation="MKKLLLLVGIIALLVACSARGMLKLPVKSENNMRLNSPLVLVKG
                     SNFFLTQDYVIDITTIDSITADAKNGISVSLSANKDSAYINATDMPQLVNISLWSKGV
                     PYGVLAKKTNKIDYVFTYDATGDKGTRYQIAGQMNDWFPAGHPDLVKNEKGLYEVTLN
                     LSPGAYLYQMVIDGDWNHDKNNPDKVDNNYGKFNSILHVKGTQEKAPKLLTQSFDENN
                     IVLSVDNAAEQVYVYWQNYLLPNDLFVKNEGQQLIVNLPKEAIETDRSFLRIFTANEY
                     GISNDVLIPLQKGKVLNNVADITRFDKHAEAIYFLLVDRFKDGNEANNFPMNRPDVNP
                     KVDYQGGDIAGITQKIKDGYFQRLGFTTIWVSPIVQNPMEPWGYWANPETKFSGYHGY
                     WPISSSKIDFRFGTDEEMHELVKTAHENNINIIVDYVANHVHKNHPLYQRDSTIATPL
                     YLPDGTKNTERWNEYRLTTWFDEFMPSIDFSRQENIDMMVDSALYWVKKFDLDGFRHD
                     ACKHVNEEFWRTLTHRMKTEVEIPRGKLTYQIGESYGSPQLISSYVNTGMLDAQFDFN
                     VFDDAASVFAFNNEDFVRVSNGLKTSLSTYGHHNLMGYISGNHDRARFISYASGDVKP
                     GENAKLAGWTRKIELTDSTAYDKLIQLNAFNLTIPGVPVLYYGDEIGDPGANDPDCRR
                     FMRFGEQLVPAEERVLNTVAQLGQLRKNNLALIYGNFIELHLERDSWAYARNYFNEVA
                     IVVFNRQTTPKTIEIELPTILQNKQYAANFGNNFTVNANKLSITVAGNGFEVLTLKK"
     gene            complement(5767..7278)
                     /locus_tag="PLJ82_02780"
     CDS             complement(5767..7278)
                     /locus_tag="PLJ82_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR40466.1"
                     /translation="MEKPRLRFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGADIG
                     NIPILWIAAPVTGLIIQPIIGHMSDKTWNKLGRRRPFFLAGAILASLALIFMPNSPYL
                     WVAAGMLWIMDASINISMEPFRAFVGDMLPAEQRTKGFAMQSFFIGTGAVIASALPYM
                     LTNWFGIANTAPEGLIPPSVKYSFYLGAIAFLVTVIWTVISTKEYSPEELATFEKNEQ
                     KENESTSNHHEAILPSSRFLKIAPIWTITGILLSVLVYFFSLEAELYILTGGITFFGL
                     ILFFVGGLVQANKQKNGLVEVINDLLLMPKTMKQLAIVQFFSWFALFAMWIYTTAAVT
                     SHIYGTSDTHSAIYNEGADWVGIMFAAYNGFAAIVAFLLPVLANYTNRRFTHVIALIA
                     GAIGLGSIYFVQNPIMLLLPMLGVGLAWSSILSMPYAILTGSLPSHKMGTYMGIFNFF
                     IVIPQIMAATILGFFVTKIAGNHAIYALLLGGVSFLIAAVMTLFVKDEDEKNI"
     gene            complement(7304..9157)
                     /locus_tag="PLJ82_02785"
     CDS             complement(7304..9157)
                     /locus_tag="PLJ82_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOR40467.1"
                     /translation="MKKTFLIVFLFVTLCLSGYAKSEKVTFERLEPASWWIGMHDTQL
                     QLMIYGKNISSSKVEFLYEGVSLVKRETTDNPNYLFLYLHIDKKTKPGTFDIVFKNGK
                     KQVGKFTYTLQARRENSAQRPSFSPADAIYLLMPDRFCNGNLQNDNVKGYIQGVNRDD
                     LGHRHGGDIEGITKKLPYLADLGVTALWITPFFDNNDKNYSYHHYATGDYYKVDPRLG
                     TKENYKELSAKCKENGLKLIIDVVPNHCGSAHWWMKDLPASDWLNKWDTYTSSNYRMT
                     AWTDPYSSEYDVKQLNKGWFSENMPDYNLANPHLFTYLKQAYIWWIEYADVDGIRVDT
                     YPYTDIRIGAKWIKAIRDEYPTINIVGECWVKTPQEIAFYQSGSMNKYGFDSHMQSVM
                     DFVLKDVFEFSFNERESWDRGMIRFYNHYAQDFLYADPNQIMNFLDNHDIDRYSEVVG
                     HDVKKYKMALALLIASRGYPQIYAGNEIMLGGNRGSYEGHRFDFPGGFPGDKRNAFTP
                     EGRTEIENDVFNYLRTLLHYRKNTTALQTGKKKQFIPYDGIYVYFRYDDNATVMVIAN
                     NNDTSRKVKTDRFVEVLAPFTKGKDVTSGKNFDITNSVEIPGKTVLLLELK"
     gene            complement(9184..10221)
                     /locus_tag="PLJ82_02790"
     CDS             complement(9184..10221)
                     /locus_tag="PLJ82_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR40468.1"
                     /translation="MKAQITIKDIARELGVSASTVSRALQDHPDISKATIELVKEYAK
                     AHNYKPNAVALSLKKQRSNIIGVVVPEMVHHFFSTVLSGIEDVANSKGYNVLVCQNNE
                     NYEKEVRMISSLISARVSGVIASISKETTNYAHFQELMDDNTPLVFFDRICTGINTDR
                     VIIDDYTGAFSAVEYLIKTGCKRIAFYSAPFTLEISKNRKNGYLDALRKYGIKVDESL
                     VHVCDTREDAIELTQQLLQEPNRPDAFFAINDATASGVLSAVKKAGLRVPEDVSICGF
                     GDGIIASTSDPLLTTVQQNGYQMGVEACNMLLKRIENELSPTQFNHRLIRTSLVVRES
                     TKPLIESPIHY"
     gene            11037..14087
                     /locus_tag="PLJ82_02795"
     CDS             11037..14087
                     /locus_tag="PLJ82_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOR40469.1"
                     /translation="MNYFNVRKSKKVALAFFFSVFFCVSAFAQQTLVKGVVEDNQGEP
                     IIGAYVTVKGAAGVGTITDYDGNFELNVSSSATLIFSYTGYQRKEIPVAGKTSLKVTL
                     AEEAIGLNEVVFTGYGSQTKREVTGSVSSVKAESFNKGIVANPVGLLQGKVAGLNITK
                     LGGGDPTNKEFNIQLRGVGSLKGNAQPLFIIDGVPGGDINSVNPNDIESIDVLKDGSA
                     AAIYGTRANAGVILITTKRGQEGKTKAEYSGSFGADVIAKKLRVLTADEYRTLVGGTD
                     EGGNTDWLSEITRPVAFSQQHNVAISGGGKGFNYRGSVGYKSNQGLAIESEYQEVLAR
                     LAANQKTLNDKLELAYDFSYSHNDKTWVDYEAFNQALKNNPTRSVFYDPSVGADPLNP
                     NYADYYNYGGYVETDGFSSYNPVSIIRGIDDDGRADVFLGSVRATLSLTNKLRLGTFV
                     SYQTGNEWTGKYWDKTTKYNEGYSRNGIASHSYKNYQNQLIENTLQYTDQIAAHSISG
                     LLGQSYQYNVWSGFSATNTDFITNLYSYNNLGAGMGVKNNDDRIGMGSYQDSDKLASF
                     FARAMYNYNQKYYVNASIRMEGSSRFGPQAHPVLGRYGLFPAISGSWRMKEESFLRSV
                     KDINDLKIRAGVGVTGNIPGTTNLYFEKVGPTGNYLWLDGEFVQPWGITSNVNENLRW
                     EKKTEYNLGIDGEFFNSKLSATLDGYYRNTTDLLWEYEMPQPPYPFGTMWDNHGQIEN
                     YGVEMAVNGNVFKNSDWKIDAGIVAAWNRNMVTRITSGYTKGKEGANLPYLNVGYLSG
                     DGLTGVNIMRLEEGKPIGNFYGYKFIRINEEGKMVYERLNSSGDVIGETTSTSSSVNM
                     QVIGNAQPLVTGGFNVNVSYKQFDLTANFRGQIGGKIFNFKRFYYENKNINLENYLLS
                     AFEGDAAKLTTDDRVFSDFYLEDGTYLRLSDVTLGYNVKTNTEIAKYFTDMRVTFTVQ
                     NAFVLTSYTGVDPEVNQGGLDPGIDGLNYYPKQRSFLLGLNFTF"
     gene            14120..15886
                     /locus_tag="PLJ82_02800"
     CDS             14120..15886
                     /locus_tag="PLJ82_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOR40470.1"
                     /translation="MKIKKLLIVGLASVGFLATQSCTDLEPELFSTILPEELKTNPEL
                     FKSQIALAYNGMVGEQGYVYREGYWNMQTGTTDEAIAPTRGKHWDDGGVHQLMHKHTW
                     TYSTREVEKGWSFWYDGVSKSNEFLKFVKSNKGEPPYDIITQSLVSEAKVLRAFYHYL
                     GMEIYGNIPIDTGLLTTPSVVQVQRANVYEWIVEEINKNLPYLEETPNYGRFTQPAAY
                     ALLARIYLNAEVYKRADVRNAKGPGDNVDYQLCIDACDAIIGGGYGYELESDYAKIFA
                     INNHTSKEIIFPIVFDAVWAPGNMFHLMTQHYVGEHINNYKTKTWNGFCTLPEFYDSY
                     LDKDAPVKITKADYIELANLAAALKSGLLADTTAYLAKKADMATKRSYCKDKRRDKTW
                     QAGLLRNEPYQVPGGVLFKKAELDVDLDSIKATESYNTFKGARFKKFEVEKGIGHHAN
                     NDFPLFRYADILLMKAEAIMRINANGGTPTSQIGVDPVALVNEVRARAGADLYTAGTL
                     TFDELLAERGRELAWEGTRRTDLIRFDKFTNTWRFKGASNPDGKQLFPSGETDRYRML
                     MPIPKWVIDRNPDVYTQNEGYK"
     gene            16010..>16784
                     /locus_tag="PLJ82_02805"
     CDS             16010..>16784
                     /locus_tag="PLJ82_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40471.1"
                     /translation="MKKVKFLFMAFVAVAMTLASCSKDEPKKPVVPVDPPKGLEEQPD
                     IAAPAAGQVTIAMRIPAGTDCYGVSFRSATGSTIHEAEKVSDTKTWYSVTVDLADATG
                     KGCLLLEDGTVSANWSTQWSVDNLEILDGPGAEFIDDFGTSNKLSCSEAGVVVYVSVG
                     SWNTVPCTPDTEYSLTVTVPACTPSDAIVYVIGGFTGSDWATAIPLEKQSNGTYTGTI
                     SGQPKAGYKYRLSESDWSSEEKVIVTEGETQTMGNKKDRS"
BASE COUNT         5050 a         3008 c         3190 g         5536 t
ORIGIN      
        1 tcacgcttgt atgcaacttg gaggtagtag ctcctccaac gagcagaggc aaggtaaaac
       61 cttgtttttc catttcttct gctaccgatg ccatttcttc gagcgagggg gttattaagc
      121 cactcaatcc gatgatatca gcgtttactt ctttggcttt gcgtaatatt tctgatgcag
      181 gcaccattac accaaggtcg ataacttcaa aattattgca ggcaagtact actcctacaa
      241 tgttcttacc aatatcatga acatcccctt ttactgtagc catcacaatt ttgccagcat
      301 tatgactgtt gctgtctttt tctgcctcga tataggggag taaaaaggca acggcttttt
      361 tcattacttg tgcacttttt accacttgtg gcagaaacat ttttcccgaa ccaaacaaat
      421 cacccacaat attcattcca tccatgagcg gaccttcgat tatttctaat gctttggcat
      481 attgtgtacg agcttctgtg aggtcggtat ctaaataatc gtaaatgcct ttaataatag
      541 cgtgtcctaa acgttcttgt aatgacttgg tacgccattc atctgttttt gtttctttgc
      601 tggtgttgct tttaacttca ttggcatagt ttatcaatcg ttctgtagca tcggctctgc
      661 gattaaaaat cacatcttct gttagttcaa gcaagtcggc tggtatgttg ctgtatattt
      721 cgagcatttc tggatttact attcccatat ccatgccttt ttgcgtagca tagtataaaa
      781 ataccgaatg catggcttct cgcactgtat tattgcctct aaatgagaag gataggttgc
      841 ttactccacc acttactttg gcataaggaa gttctttttt tatccattct actgtacgaa
      901 taaaatcaat tgcgtaatta gcatgttcct caagtccagt agcaatagct aagatattgg
      961 gatcaaaaat aatgtcttcg gcaggaaaac ctatgttagt caatagtgta taggcacgtt
     1021 ggcatacagc tatgcgtttt tcaaatgtat cggcttgacc ttgttcgtca aatgccatca
     1081 ctaccacggc tgctccatat tgacgaattt tttttgcgtg attaagaaat acttcttctc
     1141 cctcttttag cgaaatagaa ttaataattg atttgccttg tacgcatcta agaccagctt
     1201 ctaatacctc ccattttgat gaatcaatca taattggcac tttggctact gagggttctg
     1261 tagcaactaa ttgtagaaaa tcagtcatac tagcagcgga gtcgagcata gcatcgtcca
     1321 tgcaaatatc aataatttga gctcctcctt ctacttgctg acgagcaatt tgtatggctt
     1381 cttcgtattt tttatcggct attaagcgtg caaatttttt tgaccctgcc acgttagtac
     1441 gttcgccaat gtttacaaaa ttattctctt ttttgataac cattggttct aatccactca
     1501 aacgagtgaa tggttccaaa gtagtacgtt ggtgaggtgt agcactagct gcaagttgtg
     1561 caaaacgggt aatgtgttcg ggagtagtac cacaacatcc tccaataata ttaaccgctt
     1621 gatgggataa aaactcttca atttggttag acatttgctg aggtgtttca tcatacttac
     1681 ccagttcgtt cgggaaacct gcattgggat atgcactcac aaacgtatct gtcagttttc
     1741 ctaattcaat gatatgaggc aacatatcag atgctccagt cgaacagttt aagccaatag
     1801 ccaaaggttt agcgtgagac acagatatta caaaagcttc gagcgtttgt ccacttagtg
     1861 tacgggcact tttgtcgagt aaggtacctg aaataatgat gggaatttct ttgcccaagc
     1921 gtgcaaatac atcttgtatg gcatagatgg ctgcttttgc attgagcgtg tcgaaaattg
     1981 tttcgactaa caacacatct actcctcctg ccatcaatgc ttctacctgc tcagcatata
     2041 ccgttgccaa ttgttcaaac gatatttccc tatagcctgc atcttctact ttgggcgaca
     2101 tcgacagggt tttattggtc ggtccaatag ctcctgctac aaacaatggt tttgagcttg
     2161 ttgtatagtg cgtggtaact gattttgcga gttttgctgc ttcataattt aactcgtaca
     2221 ccaggttttc catcccataa tcggacatag agattcgatt ggaattaaat gtattggttg
     2281 aaataatatc cgcacctgct tctacgtatt tgcggtatat ggtttctatt atggtaggtt
     2341 gagatagggt aagtaaatca ttattcccct tttggctgtg ggtatgtgcc tgaaatcgct
     2401 ctcctctgaa atctgtttct tgcaaacgat attcttgtat catggtaccc atggcgccat
     2461 cgagtaccaa aattctttgt tctaaaatgg atgcaatatc tgtcattttc tgtgttttca
     2521 tttaacttgc aaagttacag aaaaatctaa ttaaaacaag ttaagtcatt ttttataaat
     2581 ggagaatggg tatttttgtc atattgtaat tttgtgaaag gtatttttac acagagtacc
     2641 acagaggttt cacagaggga cacggagtaa tatgtttttt tcgtgtaact tcgtgtaata
     2701 atctaacaca gagttccaca gagtattcac ggagtggcac agagttttat atcttttctt
     2761 ctcagtgtat cttcgtgttt cctttgtgtt cttcgtgaaa gaaatcttcc acagagtttt
     2821 acagagggac acggagttaa ataatttatt caaaatatga cactatctgc gtagtaatat
     2881 gtcgctccta gggggcttac attgtctaat ctaggctaat gctattatta ttccgctcct
     2941 ctggagctca actgcttact atttgtcaat ttcttaaata atagctatct ctgatgcccc
     3001 gaaggggtta catcataata gaaaatatac agtacagtac caattagctc catcggagcg
     3061 gaatagatat cttcgtgaga gagattcttt cacagagtac cacagaggtt tcacagaggg
     3121 acacagagta atatatattt ttcttcgtgt cttcttcgtg ttcttcgtgt gagacatctt
     3181 acacagagtt ccacagagta atatgttttt tttgtgtatt ttcgtgcgtt attcgtgtga
     3241 gaatagtaca cagcgatgta agtttgcata aggtttttgc actaaaaaat agaggctgcc
     3301 ttgtataaag caacctctat tcagaattat ttttttggaa tttatttctt caaggtcaac
     3361 acctcaaatc cattacctgc cacagtaatg ctgagtttgt tagcgtttac agtaaaattg
     3421 tttccaaaat tagccgcata ttgtttattt tgcaaaattg ttggtaattc aatttcaatg
     3481 gttttaggcg ttgtttggcg attaaatact acgatagcta cttcgttgaa ataattacgt
     3541 gcatatgccc acgaatctct ttcaagatgc aattcaataa aattaccata tataagtgct
     3601 aggttgtttt tacgtagctg acctaattga gccacagtgt ttaatacacg ctcttctgct
     3661 ggcactaatt gttcgccaaa acgcataaat cgtctacaat ctgggtcgtt tgcacctggg
     3721 tcgcctattt catcgccata atacaatacc ggtacacctg gaatagtaag gttaaaggca
     3781 tttagctgaa tcaatttatc ataggcagta gaatctgtta actcaatttt gcgtgtccat
     3841 ccagctagtt tcgcattttc tcccggtttt acatctcccg aagcatacga aataaaacgt
     3901 gctctgtcgt ggttgcccga aatatacccc attaaattgt ggtgtccgta ggtgcttaaa
     3961 ctcgttttta atccattgct cacacgtaca aaatcttcgt tgttaaaggc aaataccgac
     4021 gctgcatcat caaaaacgtt aaagtcaaat tgtgcatcca acatccctgt atttacgtag
     4081 ctgctaatga gttgaggact accatacgat tccccaattt gataggttag tttccctctt
     4141 ggaatttcta cttccgtttt cattctgtgt gtaagtgtac gccaaaattc ttcgtttacg
     4201 tgtttgcatg catcgtgtcg gaaaccatca aggtcaaatt ttttcaccca ataaagtgct
     4261 gaatccacca tcatatcaat gttttcttgg cgtgaaaaat caatacttgg cataaactcg
     4321 tcaaaccatg ttgtcaaacg atattcattc catctttctg tgttttttgt gccatcaggt
     4381 aaatagagag gcgttgcaat agttgaatcg cgttggtata gtgggtggtt tttatgaaca
     4441 tggttagcca cgtaatccac aattatattt atgttgtttt cgtgagctgt tttaaccaat
     4501 tcgtgcattt cttcatctgt accaaaacga aaatcaattt tagacgaaga gatgggccaa
     4561 tagccatgat agcccgaaaa tttagtttct ggatttgccc aataacccca tggctccatt
     4621 ggattttgca cgataggaga tacccaaatg gtggtaaatc ctaaacgttg gaaataaccg
     4681 tctttaattt tttgtgttat acccgcaata tcgccccctt ggtaatctac tttaggattt
     4741 acatctggac gattcatagg gaaattgttt gcttcgttac catctttaaa acggtcaact
     4801 aacaaaaagt aaatagcttc agcatgtttg tcaaaacgtg taatgtccgc cacattattt
     4861 agtactttgc ctttttgtag aggaattaat acatcgtttg atataccata ttcgttagct
     4921 gtaaatatgc gtaaaaatga tctgtctgtt tctatagctt cttttgggag gtttacaatg
     4981 agttgttgtc cctcattttt tacaaataaa tcgtttggta gaagataatt ttgccagtat
     5041 acatatactt gctctgcggc attatctaca gacaatacaa tattgttttc atcaaaagat
     5101 tgagtaagaa gtttaggtgc tttttcttgg gtacctttca catgtaagat agaattaaat
     5161 tttccataat tgttatctac cttatctgga ttgtttttat cgtggttcca gtctccatca
     5221 attaccattt ggtacaaata ggcaccagga ctcaagttta aagtaacttc gtacaatcct
     5281 ttttcgtttt ttactaaatc tggatgacca gcaggaaacc aatcattcat ttgtcctgca
     5341 atttgataac gtgtaccttt atcgccagtt gcatcataag taaatacata atcaatttta
     5401 tttgtttttt tggctaatac accataagga actcctttac tccatagcga aatatttacc
     5461 aattgaggca tgtctgttgc gttgatatac gctgaatctt tgttggctga caagctaacg
     5521 ctaatgccat ttttagcatc tgctgttatg gaatcaattg ttgtaatatc tatcacatag
     5581 tcttgagtta aaaaaaagtt acttcctttt accaacacca gcggagagtt taaccgcata
     5641 ttgttctctg attttactgg aagtttaagc ataccgcgtg ctgaacaagc cactaataat
     5701 gctatgatac cgactaaaag caataatttt ttcatgtttg tagtgtttta ttgaaatata
     5761 tataagttat atgttttttt catcttcatc ttttacaaaa agagtcatca cggctgctat
     5821 caaaaaagaa acgccgccca gtagtaaggc ataaatagca tggttacctg caatttttgt
     5881 aacaaaaaat cctaaaatag tagcagccat aatttgtgga attacaataa aaaagttaaa
     5941 tattcccatg tatgttccca ttttgtgcga tggcaatgac cctgttaaga tagcgtatgg
     6001 catggataaa atactggacc aagccaatcc cacacccaac atgggtaata acagcataat
     6061 aggattttgt acaaaataaa ttgaacccaa accaatagcc cctgctatca aagctataac
     6121 atgcgtaaag cgtctgtttg tatagttggc tagtactggc aataaaaagg caactatagc
     6181 cgcaaatcca ttgtatgctg caaacataat gcctacccaa tctgcccctt cattgtatat
     6241 agccgaatgc gtatcgctag tgccataaat atgtgatgtt actgccgctg tagtataaat
     6301 ccacatagca aatagggcaa accatgaaaa aaactgaaca atcgccaact gtttcatcgt
     6361 tttaggcatc aacaataggt cgttaatcac ttcaaccaat ccattttttt gcttgttggc
     6421 ttgtactaag cctcccacga aaaacagaat aagaccgaaa aatgtaatac cccctgtcag
     6481 aatatacaac tccgcttcca aactaaaaaa ataaactaac actgataata gtattcccgt
     6541 aatagtccat ataggagcta tttttaaaaa acgagacgat ggtaaaattg cttcgtggtg
     6601 attactcgta ctttcgttct ctttttgttc atttttctca aatgttgcga gttcttctgg
     6661 actatattct tttgtgctaa taacagtcca tattactgta acgagaaaag cgatagctcc
     6721 caaataaaac gagtatttta ccgaaggagg aatgagccct tcgggtgctg tgttggcaat
     6781 cccaaaccaa ttggttagca tataaggtaa tgcgctagct attactgctc ctgttccgat
     6841 aaaaaaactt tgcatggcaa aaccctttgt gcgttgttct gcaggtaaca tatctcccac
     6901 aaaagctcga aacggctcca tcgaaatatt aatagaggca tccattatcc aaagcatacc
     6961 agcagctacc cataaatacg gactattggg cataaaaatt aatgctaatg aggctaaaat
     7021 ggcacctgct aaaaagaatg gacgtctacg tcctagcttg ttccaagttt tatcactcat
     7081 gtgtccaatg atgggttgaa taattaaacc tgtaactgga gcagctatcc ataatattgg
     7141 gatattccca atatcggcac caagtgtttc aaatattcta cttacattgg cattttgtaa
     7201 agcaaatccg aactgtatgc ccaaaaagcc aaaactcatg ttccaaattt gccaaaaacg
     7261 taaacgaggt ttttccattt tgaatgctgt gttttttaat ttgctatttt aattctaata
     7321 ataacactgt tttacctggt atctctacgc tatttgttat atcaaagttt ttacctgagg
     7381 taacatcttt accctttgtg aaaggggcta atacttctac aaaacggtcc gtttttactt
     7441 ttcggctagt gtcattatta ttagctatta ccattactgt tgcattgtca tcatacctaa
     7501 aatatacata aatgccatcg tatgggataa attgtttttt ctttcctgtt tgcaatgctg
     7561 tggtgttttt tcggtaatgc aacaaggttc gtaaataatt aaacacatcg ttttctattt
     7621 ctgtacgccc ttcgggtgta aaagcattgc gtttatcgcc tggaaaacca ccaggaaaat
     7681 caaaacgatg accttcgtat gagcctctgt tacctcctag cataatttcg ttacctgcat
     7741 atatttgtgg gtatcctcgc gacgcgatta acagtgctaa tgccattttg tattttttta
     7801 catcatgacc cactacttcc gaataacgat caatatcgtg gttatcaagg aaattcataa
     7861 tttgattggg gtcagcatac aaaaagtctt gtgcgtaatg attgtaaaaa cgaatcatgc
     7921 ctctgtccca actttcacgt tcgttaaatg aaaattcaaa tacatctttc agtacgaagt
     7981 ccattacact ttgcatgtgc gagtcaaaac catatttatt cattgaccct gattgataga
     8041 acgcaatttc ttgaggcgtt tttacccaac attcacctac tatgttgata gtcggatatt
     8101 cgtcgcgaat agctttaatc cattttgctc ctatacggat gtctgtatag ggatacgtgt
     8161 caacacgtat gccatccaca tcggcatatt ctatccacca aatataggct tgttttagat
     8221 aggtaaacag atgaggattt gcaagattat aatctggcat attttctgaa aaccatcctt
     8281 tgtttaattg ttttacatca tattcagacg aatacgggtc tgtccatgca gtcatacgat
     8341 aattagaaga agtgtatgtg tcccatttat ttagccaatc agatgcagga aggtctttca
     8401 tccaccaatg tgcacttcca caatggttag gaaccacatc tataatgagc ttcaatccat
     8461 tttctttaca ctttgccgaa agttctttgt aattttcctt tgtgcctaaa cgaggatcca
     8521 ctttgtagta gtctcctgtg gcatagtgat ggtaagaata gtttttatcg ttattatcga
     8581 aaaatggagt tatccatagg gctgttactc ctaaatctgc caaatagggc aatttttttg
     8641 taatcccctc aatatcacca ccatgtcggt gtccaagatc atccctattt accccttgta
     8701 tgtagccttt aacgttatca ttttgtaggt tcccattaca aaaacgatcg ggcataagta
     8761 gataaattgc atctgcaggg ctaaaacttg gtcgttgagc cgagttttct ctacgtgctt
     8821 gcaaggtata ggtaaatttt cctacttgtt tttttccatt tttgaaaaca atatcaaatg
     8881 taccgggttt agtttttttg tcgatatgta agtacaaaaa caaatagttt gggttatccg
     8941 ttgtctctct cttaaccaaa gaaacacctt cgtacagaaa ttcgacctta cttgatgata
     9001 tatttttgcc atatatcatc aattgtaatt gtgtgtcgtg cataccaatc caccagctgg
     9061 caggctctaa acgttcaaat gttactttct ccgatttggc atatccactc aggcagagtg
     9121 ttacgaataa gaaaacgata aggaatgttt ttttcatata attataggtt tttttatttg
     9181 tatttagtaa tgtattgggc tttcaattaa gggttttgtc gattctctta ctactaaact
     9241 ggtgcgaatt aagcgatgat taaattgggt agggctcaat tcgttttcaa ttcgctttaa
     9301 caacatattg caggcttcaa cgcccatttg gtagccattt tgctgcaccg tagtaagcaa
     9361 tgggtcggaa gtgctagcaa taataccatc gccaaagcca catatcgata catcctcagg
     9421 cacacgcaat cctgcttttt taacagcaga caatactccc gaagctgtgg cgtcgttaat
     9481 agcaaaaaaa gcatctggtc tgttgggctc ttgcaaaagt tgttgcgtta gctcaatagc
     9541 atcttcacgt gtatcacata catgcaccaa ggattcgtcc accttaatgc cgtactttct
     9601 taacgcatct aaatagccat tcttcctgtt cttagaaatt tcgagggtaa aaggagcact
     9661 ataaaaggct attcgtttgc atcctgtttt aattaaatat tcaacagcag aaaaagcacc
     9721 cgtataatca tcaataatta ccctatcagt attaatccca gtacaaatac ggtcaaaaaa
     9781 caccaaagga gtattatcat ccattagttc ctgaaaatgg gcatagtttg tggtttcttt
     9841 agatatagaa gcaataacac cagatacgcg agccgagata agcgatgaaa tcatgcgtac
     9901 ttctttttcg tagttttcgt tattctgaca tactaataca ttgtaccctt tgctgttagc
     9961 aacatcttct ataccagata aaacagtaga aaaaaaatga tgtaccattt cgggtaccac
    10021 tactccaatg atattagaac gttgtttttt tagacttaat gctactgcgt tgggtttgta
    10081 gttgtgtgct ttggcgtatt cttttactaa ttcaatggta gctttgctta tgtctggatg
    10141 atcttgcaat gcacgtgaca cggtagatgc agaaacaccc aattctcgtg ctatgtcttt
    10201 gatggtaatt tgtgctttca tccaatatat caatttttaa ggtgttataa ttgcacaaca
    10261 atgcaataaa tacaacgccc aaaattacgt tattttttaa tatttccaaa aaaaaatgca
    10321 ggattaggaa tggaatacta gtatagtagt gttttattat acaaaatgca cgtttttata
    10381 tgcgaaaaat tattgagtgg aaatttgaaa gtggaaagtt gaaagtgttt aacgtttaac
    10441 gtttaatgtt taatgtttaa tgtttaatgt ttaacgttta gtgtttagtg tttagtgttt
    10501 ctcgtttctc gtatagcttc tgtccacttt ccactgtcaa ttgtccactc tccactgtcc
    10561 actcaccact gttcactgtc agttatccac taaaataagg agcgtagctc tgatatcttc
    10621 gtagaaaata caaacagccg ataagctagg ggcgttagcc ctgatatttt tttgtagaaa
    10681 gtttgaaagt gtttaacgtt taacgtttag tgtttagtgt ttctcgtttc tcgtatagct
    10741 tctctccact ttccactctc cattgtcaac tgtccattgt ccactgtcca ctctccactg
    10801 tcaaacagta tgaacttttt aacatttcga accatttttc tacttattct taaaaaaaat
    10861 gaataactct tgtttttgtt tgcaaaaaaa aacgtatgtt tgtcccttgt ttggtggata
    10921 ataggctgat atatcatcta tcatatagat aatcaatcag ttgttataaa aaccagttga
    10981 aaaagaagat tgtaaactaa atagatattt ttttaaccct taaaaatttt taatgcatga
    11041 actattttaa tgtaagaaaa tcgaaaaaag tggcattagc tttctttttt tcggtatttt
    11101 tttgtgtgag tgcctttgcc caacagaccc tagtgaaggg tgtggtggaa gataaccaag
    11161 gcgaaccgat tattggtgca tacgttacag tgaaaggtgc agctggagtt ggtacaatta
    11221 ccgactatga tggaaacttt gaattgaacg tttcttctag tgccacactt attttttcgt
    11281 acacaggtta tcaacgcaaa gagatacccg tagcaggtaa aacttcttta aaagtaacct
    11341 tagctgaaga agctattggt ttgaacgagg tagtatttac tggttatggc tctcaaacca
    11401 aacgtgaagt tacgggttct gtatcttctg taaaagcaga aagctttaac aaaggtattg
    11461 tggcaaaccc tgtaggatta ctacaaggta aggtagctgg tttgaatatt accaaattag
    11521 gtggtggtga cccaaccaac aaagaattca acatccaatt acgtggtgtg ggtagtttaa
    11581 aagggaatgc acaaccttta tttattatcg atggtgtgcc tggtggtgat atcaattctg
    11641 taaatcccaa cgatattgaa tctattgacg ttcttaaaga cggttcggct gcagctattt
    11701 atggtacgcg tgccaatgcg ggtgtaatct tgattacaac caaacgtgga caagaaggca
    11761 aaacaaaagc tgaatattct ggaagttttg gtgctgacgt aattgctaaa aaattgcgtg
    11821 tacttacggc agatgaatac cgtaccttag taggaggtac agacgagggt ggtaataccg
    11881 attggttaag tgaaattacc cgtcctgttg catttagtca acaacacaat gtggctatat
    11941 caggtggtgg aaaaggattt aactaccgtg gttcggtagg ctataaatct aaccaaggct
    12001 tggcgattga atctgaatac caagaagttt tggctcgttt agcagctaac caaaaaacct
    12061 taaacgataa gttagaattg gcatacgact ttagttatag ccataatgat aaaacatggg
    12121 tagattacga ggcatttaac caagcattaa aaaataatcc tacacgttct gtattttatg
    12181 atccatctgt aggtgctgat cctttgaatc ccaattatgc agattattat aattatggag
    12241 gatatgtaga aacagatgga ttctcttctt ataatccagt ctccattatt agggggatag
    12301 acgatgatgg acgtgccgat gtgtttttag gaagtgtacg tgctaccttg agtttaacaa
    12361 ataaattgcg tctaggtacc tttgtttctt atcaaactgg aaatgaatgg actggtaagt
    12421 attgggacaa aacaactaag tataatgaag gttattctcg taatggaata gcttcacata
    12481 gctataagaa ttatcaaaat caattaattg agaatacctt acagtatacc gaccaaatag
    12541 ccgctcacag tatttctggt ttgttaggtc aatcctacca atacaatgta tggagtggct
    12601 ttagtgcaac aaatacagat tttataacaa acttgtattc gtataacaat ttaggtgcag
    12661 gtatgggcgt aaaaaataat gatgaccgca ttggtatggg aagttaccaa gattctgata
    12721 agttagcctc cttttttgca cgtgctatgt acaattacaa ccaaaaatac tatgtaaatg
    12781 caagtattcg tatggaagga tcttctcgtt ttggacccca agcacatccc gtattagggc
    12841 gttatggttt gtttcctgct atcagtggta gctggagaat gaaagaagaa agtttcttac
    12901 gttctgtaaa agatattaac gatttaaaaa ttcgtgctgg ggtaggggta actggaaata
    12961 tccctggaac tacaaatttg tattttgaga aggtgggtcc aacagggaat tatttatggt
    13021 tagatggaga atttgttcaa ccatggggta ttacttctaa tgtaaacgaa aacttgcgtt
    13081 gggaaaagaa aacggagtac aaccttggta tagatgggga gttttttaat agtaaattgt
    13141 ctgctacttt agatggttat taccgtaaca caactgattt gttgtgggaa tatgaaatgc
    13201 cacaacctcc ttatccattt ggaacaatgt gggataatca tggacaaata gaaaactacg
    13261 gggtagaaat ggcagtaaat ggtaatgtat ttaaaaatag tgattggaaa atagatgctg
    13321 gaattgttgc agcttggaat agaaatatgg ttactaggat tacttctgga tatacaaaag
    13381 gaaaagaagg tgctaatttg ccgtacttaa atgttggata tctgagtgga gatgggctta
    13441 ctggagtaaa tataatgcga cttgaagaag gaaaaccaat tggaaacttt tatggatata
    13501 agtttattcg tatcaatgaa gaaggtaaaa tggtgtatga aagacttaat tcctctggtg
    13561 atgtaattgg agaaacaaca tcaacatcat caagtgtaaa tatgcaggta attggaaatg
    13621 cacagccctt ggttactgga ggtttcaatg tgaatgtatc atacaaacaa tttgatctaa
    13681 cagctaattt ccgaggacaa attggaggta agatatttaa ttttaaacgt ttttattatg
    13741 agaataaaaa cattaatctt gagaattatt tactttctgc tttcgaagga gatgctgcga
    13801 aattaacaac agacgatcgt gttttttctg atttctattt ggaagatggt acgtatttgc
    13861 gtttaagcga tgttactttg ggttataatg taaaaactaa cactgaaata gcaaaatatt
    13921 ttactgatat gcgtgtaacg tttactgtgc aaaatgcatt tgttcttact agttatacag
    13981 gcgttgaccc agaagtaaac caaggcggtt tagatccagg tattgatgga ttaaactatt
    14041 atcctaagca acgttcgttc ttactaggac ttaactttac gttctaatta atgtaatatt
    14101 attaaaaaat agaaatacaa tgaaaataaa gaaattatta atagtgggtt tggcttctgt
    14161 aggtttttta gctacacagt cgtgtacaga ccttgaacca gaattgttta gtactatttt
    14221 gcctgaagaa ttaaaaacta atcctgaatt gtttaaaagt cagatagctt tagcgtataa
    14281 tgggatggta ggtgaacaag gatatgttta tcgcgaggga tattggaaca tgcaaacagg
    14341 aacaaccgat gaggctattg ctcctacacg tggtaagcac tgggatgatg gtggagttca
    14401 tcaattaatg cataaacata catggactta tagtaccagg gaagttgaaa aaggatggag
    14461 tttttggtat gatggagttt caaaaagtaa cgagtttctg aaatttgtaa aatcaaataa
    14521 aggtgagcct ccatatgata ttataacaca atctttggtt tcagaggcta aagtattacg
    14581 tgcattttat cattatcttg gcatggagat ttatggaaat attccaattg atacaggtct
    14641 cttgacaact ccatctgttg tacaagttca gagagctaat gtatatgagt ggattgtgga
    14701 agaaataaac aaaaatctcc catatttaga ggaaacaccc aattatggtc gttttaccca
    14761 acctgctgcg tatgctttgt tagctcgtat ctatttgaat gctgaggtgt ataaacgtgc
    14821 agatgtgaga aatgctaaag gtcctggcga taatgttgat taccaattgt gtattgatgc
    14881 gtgtgatgct attattggcg gtggatatgg gtatgaatta gagtcggatt atgctaaaat
    14941 ttttgcgatt aataatcata catctaaaga aattattttc cctatcgtat ttgatgcggt
    15001 ttgggctcca ggtaatatgt ttcacttgat gacacaacat tatgtagggg aacatattaa
    15061 taattacaaa acaaaaacat ggaatggctt ttgtaccttg cccgagtttt acgattcgta
    15121 cttagataaa gatgctcctg ttaaaattac aaaagctgat tatattgaat tagctaattt
    15181 agcagctgcg ttgaagtctg ggttacttgc tgacactaca gcttatttag ctaaaaaagc
    15241 tgatatggca acaaaacgta gttattgtaa agataaacgt cgtgataaaa catggcaagc
    15301 tggtttgtta agaaatgaac cttatcaggt tcctggtggt gtcttgttta aaaaagcaga
    15361 attagatgtt gatcttgatt caattaaagc tacagaatcc tataatacgt ttaaaggtgc
    15421 tcgtttcaaa aaatttgaag tggaaaaagg gattggtcat catgctaaca acgattttcc
    15481 gttattccgc tatgctgata ttttgttaat gaaagctgag gctattatgc gtatcaatgc
    15541 caatggagga acaccaacaa gccaaattgg cgttgatcca gttgcccttg taaatgaagt
    15601 acgtgctcgt gctggtgctg acttatatac tgctggaacc ttaacttttg atgagttatt
    15661 ggctgaacgt ggtcgtgaat tagcgtggga aggtacgcgt cgtacagacc ttataagatt
    15721 tgataaattt acaaatactt ggcgatttaa aggggcttcc aatccagatg gaaaacaatt
    15781 gtttccatct ggtgaaactg accgatatag aatgttaatg cctattccta agtgggtaat
    15841 tgatagaaat cctgatgttt atacccaaaa tgaaggatac aaataagttt ttactagttt
    15901 atacaaggaa taaatttacc ttattccttg tataaataaa aaaaaatgct tttctttgta
    15961 accaaattaa aataaccttt tatttattaa tcactaaatt tatacaaaaa tgaaaaaagt
    16021 aaaatttctt tttatggcgt ttgtagcagt tgctatgacg ttagcatctt gttcgaaaga
    16081 tgaaccaaaa aaaccagttg ttccagttga tccaccaaag ggattagaag agcaaccaga
    16141 tattgctgct cctgctgcgg gacaagtaac tatagctatg cgtattcctg caggaactga
    16201 ctgctatggc gtatcattta gaagtgctac tggtagtaca attcatgaag ctgagaaagt
    16261 atctgataca aaaacatggt attctgttac agttgattta gctgatgcaa caggaaaagg
    16321 ctgtttatta cttgaggatg gcacagtaag tgcgaattgg tcaactcaat ggtctgttga
    16381 taacttagaa attttagatg gtcctggagc tgagttcata gatgattttg gaacatcaaa
    16441 taaattgtca tgttcagaag ctggtgttgt tgtttatgtg tcggttggtt cttggaatac
    16501 agttccttgt actcctgata cagaatattc tctaactgtt actgttcctg catgtactcc
    16561 atctgatgcc attgtgtacg taattggtgg ttttacagga tctgactggg ctacagcaat
    16621 tcctcttgaa aaacaatcaa atggtactta tactggtaca atttctggac aaccaaaagc
    16681 agggtataaa tatagattgt cagaatcaga ttggtcttca gaagaaaaag ttattgttac
    16741 tgaaggagag actcaaacaa tgggaaataa gaaagatcgt tcat
//
LOCUS       DAOSRP010000026        16585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3583_length_16585_cov_5.521295, whole genome shotgun sequence.
ACCESSION   DAOSRP010000026 DAOSRP010000000
VERSION     DAOSRP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_16585_cov_5.521295"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..458)
                     /gene="raiA"
                     /locus_tag="PLJ82_02810"
     CDS             complement(159..458)
                     /gene="raiA"
                     /locus_tag="PLJ82_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOR40472.1"
                     /translation="MEVKIQSIKFDASEKLEAYIQKKATKLNKKDDSISLIEVILKVV
                     KPESIENKQADIKVFAPQAELFATKTSNTFEQSVDEALEAIERQLEKYKEKKSVK"
     gene            complement(476..1360)
                     /locus_tag="PLJ82_02815"
     CDS             complement(476..1360)
                     /locus_tag="PLJ82_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOR40473.1"
                     /translation="MTNEFIDYLQYERKYSSHTVLAYKEDLLQFEQFICAQTNMCDFT
                     SIDASLARNWIVSLKQSTYSVASINRKISSLKSFYTYCKKKGIQTTNPFLRIHALKNK
                     KRLPQCIKTTEMEELLSSEDIFTKSAFENSRDKIIIELFYHTGMRRAELINLKDCDFN
                     LFSLTLRVTGKGNKQRLIPFSNSLKESITKYLDMKRKTFENPSDNFIVNNKGGQAYPM
                     LIYRIVQDYIGKVSSISKKSPHIIRHTFASALLNNGAEINAVKELLGHANLAATQVYT
                     HTTFEQLKKIYKQAHPRE"
     gene            complement(1421..1612)
                     /gene="rpsU"
                     /locus_tag="PLJ82_02820"
     CDS             complement(1421..1612)
                     /gene="rpsU"
                     /locus_tag="PLJ82_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR40474.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGVVKELRNRQAFTKPSIE
                     KRQQKIHAVYVQKLHLSEE"
     gene            1816..3597
                     /locus_tag="PLJ82_02825"
     CDS             1816..3597
                     /locus_tag="PLJ82_02825"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOR40475.1"
                     /translation="MNSITTPTSRLKLLQQSMQRCGISVCVIPHADMHQNEYISKSDA
                     YLEFYSGFTGSAGTLVVTLDFIGLWTDGRYAIQAKQEIKNTPIHLFVEGVDGVVDFFT
                     WIEQCIIEGNILGIDASLFSYNHYTKICKLCKKRNVELNLSFQPIDELWINRPKAAFQ
                     LPFILDQHFVGEYVESKLKRVRDKMSLLGATSYLLSALDEIAWLLNCRGSDIAYNPVF
                     KAYMLITHDQCVVFLNRKVCNALLFSYFSSCGVMVYDYEFILSYLQSIRNANVLFDGT
                     MVNAAMIEALDASNKKINVSSPIAQLKAIKNEVEIEGCRRAMRKDGVVWVKLWQWIET
                     ELALSNTLSEDLIAKKITQLKEKQEEFVCESFKSIVAFAQNAAICHYAISSNQLVRKE
                     SLLLVDTGTHYLHGTTDTTRVIGLGELSSMQKKEVTLVLKGHIALAQAVFLKGTKGFQ
                     LDALARQFLWKDGYDYAHGTGHGIGHCLNVHEGYASISPRAVQVALQPGMLITNEPGL
                     YKENEYGMRIENVMLVKEVGNGRSGRFLCFETLTLCPIDMQIIDKTMLTEEELIWLEQ
                     YQQKVFDELSPYLTEEERVWLKNKITN"
     gene            3641..5056
                     /locus_tag="PLJ82_02830"
     CDS             3641..5056
                     /locus_tag="PLJ82_02830"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOR40476.1"
                     /translation="MKKYCLIVCIILLPLLVVAQKKEIKAYSIELNLPHLKSEKVYLG
                     RYVMGKPYSNDSIVLDTKGRGVFQGSKPLDEGLFMLYFTDGKYVDFMVGNEQHISVKG
                     DTLHLPANLQFSGASQTKDFFQYMRFLTTLQQDYGRKQEQLKATVDDDTKKQVEKELE
                     ALSQQLLTQQNELIANYPTGMVSVFIKGLRIPDMPEDVENQATSDSLKQVMRYLFYKK
                     HYFDNVNFSDIRTYNTPYIVSNLETYLNRVLVQHPDSIVPAAIDLVEKSKGNDKTFQM
                     MTSFMLNYGVKSTMMGMDKLMVELGNRYYLTGKATWADSTLIESLRKEIKKIETSLVG
                     MKASNMYLANEKGEYSDFYSMFGEQLTVLYFFEPDCGHCKKVTPLLRSFYEKYKNDKR
                     INIVAVYMLLDKKEWSDFIEKNQLQAFTNVWDPNRVSRFWELYDASTTPMMYVMDKNK
                     KILAKKIDVETLEMIVKYELK"
     gene            5064..5579
                     /locus_tag="PLJ82_02835"
     CDS             5064..5579
                     /locus_tag="PLJ82_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOR40477.1"
                     /translation="MALIKSVRGFNPSIGKDCYLAENATIVGDVSLGEGCSVWFNTVL
                     RGDVNTIKIGNHVNIQDGAVLHTLYQKSTIEIGNYVSVGHNVTIHGASIKDYALIGMG
                     STVLDHVVVGEGAIVAAGALVLSGTIIEPHTIWAGVPAKFVKAVDPEQSKEINQRIAH
                     NYAMYASWYKE"
     gene            5672..6211
                     /locus_tag="PLJ82_02840"
     CDS             5672..6211
                     /locus_tag="PLJ82_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOR40478.1"
                     /translation="MTTEDQYQHSIATCRDVFSKKLKDYGAAWRIMRPKSITDQLFIK
                     ANRIRSIEIKGTSKIEEDIRAEFIAIVNYGIIGLIQLELGVAEVDDLPAEKALVIYDG
                     YAQKAFDLMMDKNHDYDEAWRSMRISSYTDLILMKIYRTKQIEDNDGKTLISEGVDAN
                     YFDMINYAIFGLIKLHYES"
     gene            6214..7452
                     /locus_tag="PLJ82_02845"
     CDS             6214..7452
                     /locus_tag="PLJ82_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOR40479.1"
                     /translation="MKQLVLFICRILFGLVFMFSGFVKAVDPLGSMYKFEDYFIAFGF
                     TSLAPLALFLAIALAALEFLIGANLVLGVHIKKTSWLALFFMAFMTPFTLYIAIANPV
                     ADCGCFGDAWIIDNWTTFYKNILFSILIITIFIFRNEEKIHLANRTQWMMSGFAILFS
                     VAVSVYGYMRLPIFDFRPYKIGANIIEGMTIPENAPQDEYLTTFIYEKEGKQKEFTLD
                     NFPTGDSTWTFVKQQSVLISKGFRPAIHDFSIDYQGEDITDIVLEDSNYTFLVISSKL
                     EDYSQCYSDKINKLYAYAQQNGYSFYCLTSSFGDAVENFRTLTGANYPIAFSDEVTLK
                     TIIRTNPGVLLVKNATVYNKWHCANLPDFSAPIEDTVFIDVNYPHLWKTVVWLLLLFS
                     IPVALIYVLDKKSTIHNKTN"
     gene            7465..8220
                     /gene="tpiA"
                     /locus_tag="PLJ82_02850"
     CDS             7465..8220
                     /gene="tpiA"
                     /locus_tag="PLJ82_02850"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOR40480.1"
                     /translation="MRKNIVAGNWKMNKTLQEGIALATELKDALVGTTPNCDVVIGTP
                     FIHLATVVETVKKSPIKVAAQNCADKESGAYTGEVSASMIKSTGVNYVILGHSERRAY
                     YGETFEILRNKVLLALKNELTPIFCIGELKEEREANKQNEVVKAQLEGSVFNLSATDF
                     GKIVIAYEPVWAIGTGLTATSEQAQEIHAFIRSLIADKYGKEVADSTSILYGGSCNAG
                     NAAELFSKPDVDGGLIGGASLKAVDFKAIISAF"
     gene            8342..8932
                     /locus_tag="PLJ82_02855"
     CDS             8342..8932
                     /locus_tag="PLJ82_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOR40481.1"
                     /translation="MKHTFIFATNNKHKLEEIQQIVGDKLSILSLQDMNFREDIPENE
                     PTIEGNALYKARFIYNRFGKDCFADDTGLEVVALNGEPGVLSARYAGEEKNYQSNNEL
                     LLKNLNPFPNKSARFKTVIACVLEGREYVFEGIIEGTIVNEPKGKNGFGYDPIFQPSG
                     HQQTFAELSEEVKNTISHRARAMESLFMFLQQNHII"
     gene            8958..11276
                     /locus_tag="PLJ82_02860"
     CDS             8958..11276
                     /locus_tag="PLJ82_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOR40482.1"
                     /translation="MRKIIFLFPFLFFSSIVFSQIAVGEWRMHFSYNSTTNVVVTPKK
                     VYAIADNKLFSVDKKDFSIQTYSKLSGLNSNEVSLLAYDEISNLLIVVYTNSNIDIID
                     QKGGIYNIADLYRKNMTVSKKVNHIYFDQGLAYFSCDFGIMVLNLAKKEIANTYIIGD
                     NATMSAVYAVNSDETYLYALTSESIKKAPKQGVNLLNFENWLVAMSLPDDSQLFRDMD
                     VFNNKFYVAKNNENVYVYEEGQWLVFYANDAQLAISIQTIGNHFIINASNSLLCYTKD
                     LTVEQLTDIVVSDSWYDDTEGVYWIASKTSALLRYKKGEGVLGRYKPSSPYLSTAQKM
                     YYNNGRILVAPGKSWDDRYSIPGAVLLFEDEKWTEYTSENSGVLQYSYWFADVVSIAV
                     DPVDKNKLYVSTWGEGVYVFENGVATQLFNGLNTGGALETAEPSKHNFYRIDGLVFDK
                     NKNLWMVNSLASKGLKMKTYDGKWHSFNYPSLANKFLLRHLLLHSRGQKWIVSSTSSG
                     RGIFVIDDNGTILDESDDRTRFYTSFTDRDGNVIVPSYFYSIAEDKDGTVWLGTDKGP
                     LLMTNVSKVFDNTYTCTRVKIPRNDGTILADYLLDNEEIRAIAIDAANRKWIGTGNSG
                     VYLLSANGRETIHHFTVDNSPLSSNKIESIVIQEETGEVFFSTQDGLISYRSDATVGK
                     KSFEKVEVFPNPIRPDFQGLITVRGLVANTEVRIVDQSGLLVFSGVATGGSIVWDGMN
                     FANHPVGSGVYYVFSVNADGTEHGSTKFMIIR"
     gene            complement(11273..11746)
                     /gene="folK"
                     /locus_tag="PLJ82_02865"
     CDS             complement(11273..11746)
                     /gene="folK"
                     /locus_tag="PLJ82_02865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOR40483.1"
                     /translation="MNVVYLALGSNIGNKKRILSEAESLINEKIGTVIHASSVHTTNP
                     EGFISTHKFLNKVICVYSILSPQKILETILEIEKKLGRNAKSIDGNYQDRTIDIDILY
                     YNDSIINVETLTVPHPHLHTRVFVLKPLLEIAPELIHPILQLSTKQMLLRLEKED"
     gene            complement(11752..13551)
                     /gene="typA"
                     /locus_tag="PLJ82_02870"
     CDS             complement(11752..13551)
                     /gene="typA"
                     /locus_tag="PLJ82_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOR40484.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKMLLAGSLFKEHEKVGELMMDN
                     NDLERERGITIVSKNVSIRYKDYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQIGLKPIVVINKVDKPNCRPDEVHEAVFELMFSLDASEEQLDFP
                     TIYGSAKQNWMSDSWSKQTDTIFPLLDAIIEHIPAPKKLEGTPQLLITSLDYSSYVGR
                     IAIGRIHRGTLKENMDVALVKRDGSIVKSRIKELNVFEGFNKLKVPSVSSGEICALIG
                     IDNFDIGDSITDLEKPDPLPPIAIDEPTMSMVFTINDSPFFGKDGKFVTSRHIHERLM
                     KELDRNLALQVVKSETADSWNVYGRGVLHLSVLIETMRREGYELQVGQPQVIFKTIDN
                     VKHEPIELLTINVPEEVSGKVIELVAVRKGEMTIMERKGDRVHMEFMIPSRGIIGLRT
                     NMLTATAGEAVMAHRFIEYAPYKGEIERRSNGSIIAMESGTAFAYAIDKLQDRGKFFI
                     FPQDEIYAGQVVGENSKEGDMVVNVTKSKKLTNMRASGSDDKVRIVPPIVFSLEEALE
                     YIKEDEYVEITPNSMRLRKILLDENDRKRAKAK"
     gene            complement(13616..14449)
                     /gene="murI"
                     /locus_tag="PLJ82_02875"
     CDS             complement(13616..14449)
                     /gene="murI"
                     /locus_tag="PLJ82_02875"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOR40485.1"
                     /translation="MRKGSRISRIGVFDSGYGGLTILEEFRKKLPEYDYVYLGDNART
                     PYGTRSFEVVYQYTLECVKKLAKMDCELIILACNTASAKALRTIQQKDLPILFPQKRI
                     LGVIRPTVEQLDTCTQSKHVGILGTEGTIQSQSYTIEINKLYPHITVVDQACPMWVPL
                     IENNEHESDGADFFVHKYLSNLLNKDKFIDAIVLGCTHYPLLLKKIKHILPSHINIIS
                     QGEIVAQSLENYLLRHPEIETKLAKNHQCMFYTTESELKFISSASIFLKETIKAERIS
                     L"
     gene            complement(14518..15036)
                     /locus_tag="PLJ82_02880"
     CDS             complement(14518..15036)
                     /locus_tag="PLJ82_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR40486.1"
                     /translation="MKKSIIISLCIAFSFSLFAQTNDVPKFGHINKQDVFSLMPEADA
                     AMKELEALLLNYKVESKKLEEEFTKKQQDYVANQATLDAAIKKYRETELQRLYASIEE
                     FSKNADEALKKRQSELLAPIEAKINDAIKTIGDENGFIYIFDVTTPGTFAYMSTKSVD
                     ILPLVKQKLNLK"
     gene            complement(15070..15585)
                     /locus_tag="PLJ82_02885"
     CDS             complement(15070..15585)
                     /locus_tag="PLJ82_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR40487.1"
                     /translation="MKKTLLLVGIFVLSFTAMQAQRFALVDIEYILQQIPTYESATEQ
                     LNQSSKQWEKEIEALTTEAQTLYKNYQTEMVFLSDEMKVKREEAIIAKEKEAMDLKKK
                     YFGAEGELFKKKESLLKPIQDEIYNAVKEISENKNIQLILDRASGNNIIFASPTIDIS
                     DEVLQRMGYVR"
     gene            complement(15607..16413)
                     /locus_tag="PLJ82_02890"
     CDS             complement(15607..16413)
                     /locus_tag="PLJ82_02890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR40488.1"
                     /translation="MRYIVRYVVYLPIDIMDRLKGNRNKMIPPRGMMYIGSGDFEIQG
                     KRILSLVQSYTHLQPHHHVLDVGCGIGRFAIPLTSFLSTNGTYEGFDIVKTGIDWCKK
                     HITPSYPHFNFSHIDLNNTLYNTATKAKADQFKFPYSKSYFDIVVLTSVFTHMMPNDV
                     ANYIEQISDVLKPEGMCYATFFLFNKQENFSSTERSFQFSYDYGHYRLLDKKVKEANI
                     AFDEAFIHSLAEKHQLSIKHSIKGWWRGIEKTDKIDFQDIIIFEKRNQLS"
BASE COUNT         5211 a         2408 c         3133 g         5833 t
ORIGIN      
        1 ttcgaaccca cgaccccgag attacaaatc acgtgctctg gccaactgag ctatgaaggc
       61 atttttcggc taaaaagccg ttctaacaaa aatcttgcta aaacggcttg caaatgtaga
      121 gctttttttt ggaataccaa aacaaattgt aaaaaaaact atttaactga ttttttttct
      181 ttgtattttt ctaattggcg ttctattgcc tctagggctt cgtcaactga ttgctcaaaa
      241 gtattgcttg tcttggttgc aaataattct gcttgtggag caaagacctt aatgtcggct
      301 tgtttatttt cgatgctttc tggttttact actttaagta ttacttctat taatgaaatg
      361 gaatcatctt ttttattcag ttttgtggct tttttttgaa tatacgcttc taatttttct
      421 gaggcgtcaa atttaatgct ttgaattttt acttccataa ttgtaatttt tttagttatt
      481 ctcttggatg agcttgttta tatatttttt ttaattgttc aaatgttgta tgtgtatata
      541 cttgtgttgc ggctaagttt gcatgaccga gtagctcttt cacggcattt atttcagctc
      601 cattatttag tagtgcactt gcaaatgtat gtcgaatgat atgagggctt ttttttgata
      661 ttgagctaac ttttccgata taatcttgca caatacgata aattaacatt gggtatgctt
      721 gtcctccttt gttatttacg ataaagttat cggaagggtt ttcaaaagtt tttctcttca
      781 tatccaaata cttagtaatt gattctttga gtgaattact aaaaggaata agcctctgct
      841 tatttccctt tccagttaca cgcaaagtta aggaaaataa attgaaatca caatctttta
      901 ggttgattaa ttctgctctt cgcatgccag tatggtaaaa taattcaatg ataattttat
      961 ctcgagaatt ttcaaatgct gattttgtaa aaatatcttc acttgaaagt aattcttcca
     1021 tttcagttgt ctttatgcat tgaggtagtc tctttttgtt ttttagagca tgaatgcgca
     1081 gaaaaggatt ggttgtttgt attccttttt ttttgcagta ggtataaaaa gatttaagtg
     1141 aggatatttt tctattgata ctagcaacac tatatgtaga ttgctttagt gaaactatcc
     1201 aatttcttgc caaagaggca tcgatggaag taaaatcgca catgttggtc tgggcacaga
     1261 taaattgttc aaattgcaga agatcttcct tatatgcaag aacagtatga gaagaatatt
     1321 tcctctcata ctgtaaataa tctatgaatt cgtttgtcat acaaatgagt gtctatttct
     1381 cagaaacaaa tgtacaacat ttatgctaga aatacaaaaa ttactcttcg cttaagtgaa
     1441 gtttttgtac gtagactgcg tgaatttttt gttgtctttt ttcaatagaa ggtttagtaa
     1501 aagcttgacg gtttctaagt tcttttacta ctcctgtttt ttcaaatttt cttttaaatt
     1561 tttttagggc tctttcaatg ttttcgcctt ctttaactgg tacaataatc atttcttctt
     1621 gtttttaata atgatgtata attttgaggg gacaaaaata cagaatcatt ttttgattga
     1681 caaatattta ttcatctttt ttttgttttc tatttttaaa aaaatgtcaa aaaatgtttt
     1741 tatagggttt ttgattgaaa aagctagatg taattacaat aaattaatgt aattttgttg
     1801 gtcaaatttg tataaatgaa tagtataact acaccgacta gcagattaaa attgttgcaa
     1861 caaagcatgc aacgatgtgg tatatcagta tgtgtgattc cacatgctga tatgcatcag
     1921 aacgaatata taagtaagtc ggatgcttat ttagaatttt attcaggttt tactggttcc
     1981 gcagggacat tggtagtaac tttagatttt ataggattat ggacagatgg acgttacgca
     2041 attcaggcaa aacaagaaat aaaaaatacg cccattcatc tttttgtaga gggagtggat
     2101 ggggtcgtcg atttttttac atggattgag cagtgtatta tagaagggaa tattcttgga
     2161 atagacgctt ccttgttttc gtataatcac tatacaaaaa tttgtaaatt atgtaaaaaa
     2221 cgcaatgtag aacttaatct ttcctttcag ccgatagatg agctatggat aaatagacca
     2281 aaggctgctt ttcaattacc ctttatatta gatcagcact ttgttggtga atatgtagaa
     2341 tccaagttaa aaagagtaag agacaaaatg tctttattgg gagctactag ttatttattg
     2401 tctgctttgg atgaaatagc ttggttgctg aattgcagag ggagtgatat tgcatataat
     2461 cctgttttta aggcgtatat gttgataact catgatcaat gtgtggtatt tttgaataga
     2521 aaagtctgca atgcattgct gttttcgtat ttttcgtcgt gtggagtgat ggtatatgac
     2581 tatgaattta ttttatcgta tttgcaatca atacgaaatg ccaacgtatt gtttgatgga
     2641 acgatggtga atgcagctat gattgaggct cttgatgcat ccaataaaaa gattaatgtt
     2701 tcttctccta ttgcacaatt aaaagcaatc aaaaatgaag tagaaataga aggttgtagg
     2761 cgtgctatgc gtaaggatgg tgtagtctgg gttaagttat ggcaatggat tgaaactgaa
     2821 ttagccttat ccaatacatt atctgaggat ttaattgcaa aaaaaataac tcaactgaag
     2881 gagaaacaag aggagtttgt gtgtgaaagt tttaaatcaa ttgttgcgtt tgcacaaaat
     2941 gctgctattt gtcattatgc tatttctagt aatcaattag ttagaaaaga gagcttacta
     3001 ttggtagata cgggaacaca ttatcttcat ggtactacag atacaacacg tgttattggc
     3061 ttaggcgaat tgtcttctat gcaaaaaaaa gaagtaacct tggtattaaa aggtcatatt
     3121 gcattggcac aagctgtatt tttaaaaggg acaaaggggt ttcagttaga tgctttagct
     3181 cgtcagtttt tgtggaaaga tggctacgat tatgcacatg gcactggaca tggtataggg
     3241 cattgtctga atgtgcacga aggttatgct tcaattagtc ctcgtgcagt acaagtggca
     3301 ttgcagcctg gtatgttaat tacgaatgaa cctgggttgt ataaagaaaa cgagtatgga
     3361 atgcgtattg aaaatgtaat gttagtcaag gaagtgggta atggtaggag tggtagattt
     3421 ttgtgttttg agacgctaac gttgtgtcct attgatatgc agattataga taaaacgatg
     3481 ttgacagagg aggaacttat ctggctagaa caatatcaac aaaaagtatt tgatgaatta
     3541 tcgccctatt tgacagaaga agaacgagta tggttaaaaa ataaaataac aaattaatga
     3601 gatacataat tttatagaga gaaattaaat taaaagcatc atgaaaaaat attgtttgat
     3661 agtatgtatt attctactcc cattgttagt tgtagcacaa aagaaagaga ttaaagctta
     3721 ttctattgaa ctcaatttgc ctcacctaaa aagtgagaag gtgtatttgg gaagatatgt
     3781 tatggggaag ccatattcca acgattctat tgtgttggac acaaaagggc gtggcgtttt
     3841 tcaaggatca aagcccttag atgagggatt gtttatgttg tattttacgg atggcaaata
     3901 tgttgatttt atggttggga atgaacagca tatttcggtg aaaggagata cgttacattt
     3961 gccagcaaat cttcagttta gtggagcaag ccaaacaaaa gatttttttc agtatatgcg
     4021 ttttttaacc acattgcaac aggattatgg tcgtaaacaa gaacaactaa aagcgacggt
     4081 ggatgacgat actaaaaaac aagtagaaaa agagttggag gctttaagtc aacagttact
     4141 tacacagcaa aatgagctca ttgctaacta tcccactggt atggtctctg tttttattaa
     4201 aggtttacgt ataccagata tgccagaaga tgttgaaaat caagcaacaa gtgatagtct
     4261 gaaacaggtg atgcgttatt tattttacaa aaaacattac tttgacaatg ttaatttttc
     4321 tgatattcgt acgtataata ctccgtatat tgttagtaat ttagaaacct atctaaatag
     4381 ggtacttgtg caacatccag actccattgt tcctgcagcg attgatttag ttgaaaaaag
     4441 taaagggaat gataaaacgt ttcagatgat gacaagtttt atgcttaatt atggtgttaa
     4501 gagtactatg atgggaatgg acaagcttat ggtagaattg ggcaataggt attatttaac
     4561 aggaaaggcg acttgggcag attctacttt aattgaaagt ttacgaaaag aaattaaaaa
     4621 gatagaaacg tccttggtcg gtatgaaggc ttctaatatg tatttagcta atgaaaaagg
     4681 ggagtactct gatttttatt ccatgttcgg agaacagctg acggtattgt atttttttga
     4741 gccagattgt ggtcattgta agaaggttac accattatta cgtagttttt atgagaaata
     4801 taaaaatgat aaaagaatca atattgtagc agtatatatg cttttagata agaaagaatg
     4861 gtctgatttt attgaaaaaa atcaattaca agcatttacg aatgtttggg atccaaaccg
     4921 agtttctcgg ttttgggagc tttatgatgc ttctactaca cccatgatgt atgtgatgga
     4981 taagaataaa aagattttag caaaaaaaat agatgttgaa actttggaaa tgattgtaaa
     5041 atacgaactt aaataaaaaa acaatggctt taataaaatc agttagagga tttaatccaa
     5101 gcataggtaa agactgttac ttggcagaaa atgccacgat tgtgggtgat gtgtcgcttg
     5161 gagaaggttg tagcgtgtgg tttaatactg tattacgtgg cgatgttaat acaataaaaa
     5221 taggtaatca tgtaaatatt caagacgggg cagttttaca tacattgtac caaaaatcta
     5281 cgatagaaat aggtaattac gtttctgttg gacataatgt tacgattcat ggagcaagca
     5341 ttaaagacta tgctctaata ggtatgggta gtacggtact cgatcatgta gtagtaggag
     5401 aaggggctat tgtagcagct ggtgccttgg tattaagtgg cactattatt gagccacata
     5461 ctatttgggc tggtgtgcct gcaaaatttg tgaaagctgt tgacccagag caatcaaaag
     5521 aaattaatca acgcatagct cataattatg ctatgtatgc aagttggtat aaggagtaaa
     5581 aaatgttgaa gatttgtttt aataataaat tctatttttg ttttgattga aaaagtgtaa
     5641 ctttgtggct tattatcaac gtatttaacc catgacaaca gaagaccaat atcaacatag
     5701 tattgcaacg tgcagggatg ttttctcaaa aaaattaaaa gattatggtg cggcttggcg
     5761 tataatgcgt ccaaaatcta ttacagatca gctttttatt aaagccaatc gtataaggag
     5821 tattgaaata aagggtactt caaaaattga ggaagatatt cgtgctgaat ttatagctat
     5881 agtaaattat ggtattattg gtttgataca gttagagtta ggtgttgcag aagtagatga
     5941 tttaccagca gaaaaagcct tggtaatcta cgatggctat gctcaaaaag ctttcgattt
     6001 gatgatggat aaaaatcatg attatgacga agcttggcgt agtatgcgta ttagttcgta
     6061 tacagattta atactgatga agatatacag aacaaaacaa attgaagata acgatgggaa
     6121 aacgttaata tcagaagggg tggatgccaa ctattttgat atgattaatt atgccatatt
     6181 tggattaatt aaattgcatt acgaatctta gagatgaaac aattagtgtt atttatttgt
     6241 agaattttat ttggactggt gtttatgttt tcaggctttg ttaaggctgt ggatccactt
     6301 ggttcgatgt acaaatttga agattatttt atagcctttg gttttacttc tctggctccg
     6361 ttggcactat ttttggctat tgcgttggct gcattagagt ttcttattgg agctaaccta
     6421 gtattagggg tacatattaa aaaaacatca tggttggcgt tgtttttcat ggcttttatg
     6481 actcccttta ctttatacat tgctattgct aatccagtag ccgattgtgg atgttttggc
     6541 gatgcttgga ttattgataa ttggacgacc ttttacaaaa atatcttatt ttctattttg
     6601 attatcacta tttttatttt tcgtaatgag gaaaagattc atctggcaaa cagaacacaa
     6661 tggatgatga gtggttttgc tatattattt agtgttgctg tttctgtgta tgggtatatg
     6721 cggttgccaa tatttgattt tcgtccgtat aaaattggag caaatatcat tgaaggaatg
     6781 accattccag agaatgcccc acaggatgaa tatctcacta cctttatata cgaaaaagaa
     6841 ggaaagcaaa aggagttcac tttagataat tttccaacag gagattctac ttggacattt
     6901 gtgaaacaac aatccgtact tatatcaaaa ggttttcgtc ctgcaataca tgatttttca
     6961 attgattatc aaggagagga tattacagat attgtattgg aagatagtaa ttatactttt
     7021 ttagtgataa gctctaagtt agaagattat agtcagtgtt attctgataa gataaacaag
     7081 ttgtatgctt atgcacaaca aaatggatat tcgttttatt gcctaacgtc atcttttggc
     7141 gatgcagtag aaaattttag aacgctaaca ggggcaaatt atccaatagc attttctgat
     7201 gaggtaacac tgaaaaccat tataagaaca aatccaggtg tattgttggt gaaaaatgca
     7261 accgtgtata ataagtggca ttgtgcaaat cttcccgatt tttcagcccc gatagaggat
     7321 actgtattta ttgatgtgaa ttatcctcac ttatggaaga cggtagtgtg gttactttta
     7381 ttatttagta tacctgtagc tctaatatat gtgttagata aaaaatcaac gattcataat
     7441 aaaacaaatt aaacaaaaac gaaaatgaga aaaaacattg tagcaggaaa ctggaaaatg
     7501 aacaaaaccc tacaagaggg tattgcatta gcaacggagt taaaggatgc tttggtagga
     7561 acaacaccta attgtgatgt tgtaattggc acacccttta ttcatttagc aacagtagta
     7621 gaaactgtaa aaaaatcacc tataaaagta gcggctcaaa attgtgctga taaagaatca
     7681 ggggcttata ctggagaggt ttcagcttct atgattaaat ctacaggcgt taattatgtg
     7741 attttgggac attcagaaag aagagcttat tatggcgaaa cttttgaaat tttaagaaat
     7801 aaagtattgc ttgcattaaa aaatgaactt acacctatat tttgtattgg cgaattaaag
     7861 gaagaacgtg aagcaaataa acaaaatgaa gtagtaaaag cccaattaga aggttcagtt
     7921 tttaatttgt ctgctacaga ttttggtaaa atagttattg cttatgagcc tgtatgggct
     7981 attggaacag gattaactgc aacctcagaa caagcacaag aaatacatgc ttttattcgt
     8041 agtttaattg cagataaata tggcaaagaa gtagctgata gcactagtat cctttatgga
     8101 ggtagttgta atgcaggaaa tgcagcagag ttattttcta agccagatgt agatggagga
     8161 ttaattggtg gagcatcgct taaagcagtt gactttaaag caattattag tgcgttttaa
     8221 ttggttttgg taattaaatt taagccatcc ttttgtaagg atggcttttt tgtctaaata
     8281 gttttataaa tccgaatata aaggtatttt tgtaaggtaa aacttgatat tttgaattag
     8341 gatgaaacat acatttattt ttgctacaaa taataaacat aaattagaag agattcagca
     8401 aatagttggg gataaattat caatcttgag tttacaagat atgaacttta gagaggatat
     8461 tccagagaat gaacctacta tcgaagggaa tgcattgtat aaagctcgat ttatttacaa
     8521 tcgttttggc aaagattgtt ttgctgatga tacgggtttg gaggtagttg cgttgaatgg
     8581 tgaacctggc gttttatcag cacgttatgc gggagaagaa aaaaattatc agtccaataa
     8641 tgaattattg ctaaaaaatc ttaacccatt tccgaataag tcagcacgtt ttaaaactgt
     8701 gattgcgtgt gtgttagagg gaagagagta tgtgtttgag ggtataattg agggtactat
     8761 tgttaatgaa ccaaaaggga aaaatggatt tggatatgat ccgatttttc aaccatctgg
     8821 acatcaacag acttttgcag aattatctga agaagttaaa aatacaatta gtcatcgggc
     8881 tagagccatg gaaagtttat ttatgttttt gcaacaaaat catattattt aaatcaaagc
     8941 aaataaatct ttatagtatg cgaaaaatca tctttttgtt tccatttcta tttttttcta
     9001 gtatcgtatt ctctcagatt gctgtgggag agtggcgtat gcacttttca tataattcta
     9061 cgaccaatgt tgtagtaaca cctaagaaag tgtatgctat agcagataat aaattgtttt
     9121 cggtggacaa aaaagatttt tctattcaaa cctattctaa actgtcggga ttgaactcaa
     9181 atgaagtttc gttgttagct tatgatgaga ttagtaattt gctaatagtg gtatatacaa
     9241 actccaatat agatattatt gatcagaagg gaggtatcta taatattgct gatttgtatc
     9301 gaaaaaatat gacagtaagc aaaaaagtta atcatattta ttttgatcaa ggtttagcgt
     9361 acttttcgtg tgattttgga attatggtat taaacttagc taaaaaggaa attgccaata
     9421 cctatatcat tggagataat gccactatgt ctgcagtata tgctgtaaat tccgatgaaa
     9481 cgtatttata tgcacttacc agtgaatcta ttaaaaaagc acctaaacaa ggtgtaaatt
     9541 tattgaattt tgaaaattgg ttggttgcta tgagtttgcc tgatgattcg cagttattta
     9601 gagatatgga tgtatttaat aataagtttt atgtcgccaa aaataatgag aatgtatatg
     9661 tgtatgaaga aggtcaatgg ctagtgtttt acgctaatga tgcacagcta gctatttcta
     9721 ttcaaacgat aggtaaccat tttattataa atgcttccaa ttccttgttg tgttatacga
     9781 aagatttaac cgtagaacaa ctgactgaca tagtagtatc tgattcgtgg tacgatgata
     9841 cagaaggtgt ttattggatt gcatcaaaga catcagcact tcttcggtat aaaaaagggg
     9901 aaggggtatt aggtagatat aagccgtcat ctccgtattt aagtactgct caaaagatgt
     9961 attataacaa tggtagaata ttggttgctc ctggcaaaag ttgggatgat agatatagta
    10021 tacctggagc agttttatta tttgaggatg aaaaatggac tgaatataca tctgagaatt
    10081 cgggagtgtt acagtattct tattggtttg cagatgtggt ttctatagca gttgatcctg
    10141 tggataagaa taaattatat gttagtacat ggggagaagg ggtgtatgtg tttgaaaatg
    10201 gtgtggctac tcaattgttt aatggtttaa atacaggagg tgcgttagaa acggcagagc
    10261 cgagcaaaca taatttttat agaatagatg gattagtttt tgataaaaat aaaaatttat
    10321 ggatggttaa ttctcttgct agtaaaggtt taaaaatgaa aacttatgat ggaaaatggc
    10381 attctttcaa ttatcctagt ttagcaaata aatttctgtt aaggcattta ttattgcatt
    10441 ctcggggtca aaagtggatt gtttcgtcta caagttcagg gagaggaatt tttgtgatag
    10501 atgataatgg aaccatttta gatgagtcgg atgatagaac tcgtttttat acttctttta
    10561 ctgatagaga tggaaatgtt attgtccctt cttattttta ttcgatagct gaagataaag
    10621 atggaacagt gtggcttgga acagataaag gtccattgtt gatgaccaat gtgagcaaag
    10681 tatttgacaa cacatataca tgtactaggg ttaaaatccc taggaatgat ggtacaatat
    10741 tggctgatta tttattagat aatgaagaaa tacgtgctat agcaattgat gcagctaata
    10801 gaaaatggat aggcacaggc aattcaggag tatatctatt gtcagcaaat ggtcgggaaa
    10861 caattcatca ttttactgta gataatagtc cattgtcttc gaataaaatt gaatccatcg
    10921 ttattcaaga agaaactggg gaagtgtttt tttctacaca ggatggtcta atttcttatc
    10981 gttcagatgc tacagtagga aaaaaaagtt ttgaaaaagt agaagtattt cctaatccta
    11041 tacgacccga ttttcagggg ctaattactg ttagagggtt agttgccaac acagaagttc
    11101 gtattgttga tcagtcaggt ttgttggtct tttctggtgt agctactggg ggttcaattg
    11161 tgtgggatgg gatgaatttt gccaatcatc ctgttggctc tggtgtatat tatgtatttt
    11221 ctgttaatgc cgatggcaca gagcatggct caactaagtt tatgattatt cgttagtctt
    11281 ccttttcaag tcttaaaagc atctgttttg tagatagttg aagaattgga tgtatcaatt
    11341 ctggggctat ttctaaaagg ggttttaata caaacactcg tgtatgtaaa tgtggatgag
    11401 gaactgttag ggtttcaacg ttaataatag aatcattata atataaaatg tcaatatcaa
    11461 ttgttctatc ttggtagttt ccatctatag atttggcatt ccttccaagt ttcttttcga
    11521 tttcgagaat ggtctctaag attttttgtg gacttaaaat agaatacaca caaattactt
    11581 tattaagaaa tttgtgtgta gaaataaatc cttcagggtt tgttgtatgg actgaagacg
    11641 catgtataac tgtaccaatt ttctcgttaa taagggattc agcttccgat agaattcgct
    11701 ttttattccc aatattactt cctagagcta ggtatacgac attcattgat tttattttgc
    11761 tttcgctctt tttctgtcat tttcgtcaag aagaattttt cgcaatcgca ttgaatttgg
    11821 tgttatttca acatattcat cttctttaat gtattctaaa gcctcttcta aactaaatac
    11881 aataggagga acgatgcgta ctttgtcatc cgaaccagat gcacgcatat tcgttagttt
    11941 cttcgatttc gttacattta ccaccatatc tccttcttta gaattttcac caactacttg
    12001 tccagcatat atttcgtctt gaggaaaaat aaagaacttt cctctatctt gtaatttgtc
    12061 aatagcatac gcaaatgcag taccactttc cattgcaata attgatccat tgcttcttct
    12121 ctcaatttca cctttgtaag gagcatattc tataaaacga tgagccatta cagcttcacc
    12181 tgctgtagcc gttagcatat tggttcgtaa gccaataatc cccctagagg gaatcataaa
    12241 ttccatgtgt acccgatcac ctttacgttc cataatagtc atttctcctt tacgcacggc
    12301 aactaactca attactttac cagaaacttc ttctggtaca tttatggtta ataattcaat
    12361 aggttcatgt tttacattat caattgtctt aaaaataacc tgtggctgtc ctacttgtaa
    12421 ttcatatcct tcgcgtcgca ttgtttcgat taatacggat aagtggagta ctccacgtcc
    12481 atatacgttc cacgaatcgg ctgtttctga ttttacaact tgtaatgcta agtttctgtc
    12541 taattctttc atcaaacgct cgtgaatatg ccgtgaagta acaaattttc catccttacc
    12601 aaaaaatgga gagtcgttaa ttgtaaatac catactcatt gtaggttcgt cgatagcgat
    12661 aggaggtagc ggatctggtt tttcaagatc agttatgcta tcgccaatat caaaattatc
    12721 aataccaatt aaggcacaaa tttcacctga ggatacactt ggtactttta atttgttaaa
    12781 cccttcaaat acatttaatt cttttattcg agattttacg atacttccat cgcgtttaac
    12841 taaagccaca tccatatttt cttttaatgt gcctctatga atgcgtccaa ttgctatacg
    12901 acctacatac gatgaataat ctaaggatgt tattaataat tgaggggtac cttccagttt
    12961 tttaggagca ggaatatgtt caatgatagc atctagtaaa gggaaaatag tatctgtttg
    13021 tttggaccaa gagtcagaca tccagttctg ttttgcagag ccataaatag ttgggaagtc
    13081 tagttgttct tctgaagcat ccaaactaaa catgagttcg aatactgctt cgtgtacttc
    13141 atctgggcga caatttggtt tatctacttt gtttattaca acgattggct ttagacctat
    13201 ttgaatggct ttttgcaaca cgaaacgtgt ttgtggcatt ggaccttcaa atgcatccac
    13261 taatagcaac acaccatcag ccatatttaa cacacgttct acttcaccac caaaatcgga
    13321 gtgaccagga gtgtcaatga tattaatttt gtaatcttta tagcggatag aaacattttt
    13381 agatacaatt gtaatgcctc gttctcgctc aaggtcatta ttatccatca ttaattctcc
    13441 cactttttcg tgttctttga acagtgaacc cgctaataac attttatcaa ctaatgttgt
    13501 ttttccgtgg tcaacgtgtg caataatcgc aatatttcta atcttttgca tatagttttt
    13561 atttgaggat gcaaaggtag tcagaatatt tttatctgta ctataatttt gattattaca
    13621 gcgaaattct ttctgctttt atcgtttctt ttaagaagat ggaggcggag gaaataaatt
    13681 taagctcaga ttcagtagta taaaacatac attggtggtt tttagctaat tttgtctcta
    13741 tttcaggatg acgcaaaaga tagttctcta aactttgagc aacaatttcg ccctgagaga
    13801 taatattgat atgtgacggt aagatgtgct taattttttt caataacaat gggtaatgtg
    13861 tacatcccaa tacaatcgca tcaataaatt tgtctttgtt aagtaagttt gaaagatact
    13921 tatgcacaaa aaaatcagca ccatcacttt catgttcatt gttttcaata agaggcaccc
    13981 acatcggaca agcttgatcc actactgtaa tatgcggata tagtttattt atctctatag
    14041 tatacgattg agattgaatg gtgccttccg ttcccaaaat cccaacatgt ttagattggg
    14101 tgcaagtgtc tagttgttct actgttgggc gaattacccc tagtattcgt ttttgcggaa
    14161 atagtattgg caagtctttt tgttgaatcg tacgtagtgc ttttgccgaa gctgtattgc
    14221 aagccaaaat aattaactca caatccatct tggctagttt ctttacgcat tccagtgtgt
    14281 actgatacac tacttcgaac gaacgtgttc catatggcgt tcgagcatta tctccaaggt
    14341 aaacataatc atactcgggt agtttttttc taaactcctc taagatagtt aatcctccat
    14401 aaccagagtc aaaaacacct atacgggata ttctacttcc tttacgcata taaaacaaca
    14461 ggaagataga gtacactatt tgtctacttt tcttcctgtt tgctatcagt aaattgttta
    14521 ttttaaattc aacttttgtt ttactaatgg taaaatatct acacttttgg tcgacatata
    14581 agcgaatgta ccaggtgtgg ttacatcaaa aatatagata aatccgtttt cgtctccaat
    14641 cgttttgata gcatcattta tttttgcctc aatcggtgct aataattctg attgtctttt
    14701 ctttagggct tcgtctgcat tttttgaaaa ttcttcaata cttgcatata aacgttgtaa
    14761 ttctgtttct ctatattttt ttatagcagc atcaagtgtt gcttgatttg caacataatc
    14821 ttgttgtttc tttgtaaatt cttcctctag ctttttactt tctactttgt agttaagcaa
    14881 taaagcttcc agctctttca ttgctgcatc ggcttctggc attaaagaaa agacatcttg
    14941 tttattgata tgaccaaatt ttggtacatc atttgtttgt gcaaataaag aaaaagaaaa
    15001 tgcgatacat aagctaataa tgatgctttt tttcataatg tttttttatt ttaatgagta
    15061 aaattggttt tatcgtacat atcccatgcg ttgtaatact tcgtcgctaa tatcaatagt
    15121 aggggatgca aaaataatat tatttccaga cgctctgtcc aaaattaatt gaatattttt
    15181 attttctgag atttctttta cagcattata aatctcatct tgaataggtt tgagaaggct
    15241 ttcttttttc ttaaatagtt ctccttcagc accaaaatat ttctttttca aatccattgc
    15301 ttctttttct tttgcaatga ttgcttcttc tctttttacc ttcatttcat ctgataaaaa
    15361 taccatttct gtttgataat ttttatacaa tgtttgagct tcagtggtga gagcttcgat
    15421 ttccttttcc cattgttttg aactttggtt aagttgttct gtagcagact cgtaagttgg
    15481 tatttgttgc aatatatatt cgatatctac caatgcaaat cgttgagcct gcatcgctgt
    15541 gaaactaagg acaaaaatgc ctactaatag aagtgttttt ttcatgtctt ttacatttta
    15601 agattactaa ctaagttgat ttcttttttc aaaaataata atatcttgaa aatcaatttt
    15661 atctgttttt tctatacctc gccaccaacc tttaatactg tgtttaatac taagttggtg
    15721 tttctcagct aaagagtgaa taaatgcttc atcaaatgca atattagctt cttttacttt
    15781 tttatcaagc aagcgataat gtccgtagtc gtaactaaat tgaaagctcc tttctgttga
    15841 ggagaaattt tcttgtttat taaataggaa gaatgtggca taacacatcc cttctggttt
    15901 taatacatca ctaatctgtt ctatataatt agcaacatcg tttggcatca tatgtgtgaa
    15961 taccgatgtt agaactacga tatcgaagta cgactttgaa taaggaaatt taaattgatc
    16021 agcttttgct ttggtggcag tgttgtataa ggtattgttt aagtcaatat gtgaaaaatt
    16081 gaaatgtgga tatgacggag taatatgttt tttgcaccaa tctatacctg ttttaacaat
    16141 atcaaaaccc tcataagtgc cattggttga taaaaaagat gtgagtggta tagcaaatcg
    16201 cccaattcca catcctacat ccaatacatg atggtggggt tgaaggtgcg tatatgattg
    16261 aaccaaagaa agaatacgtt taccttgtat ttcaaaatcg cccgaaccta tatacatcat
    16321 ccctctcggt ggaatcatct tgtttctgtt tccttttagt ctatccatta tgtctatagg
    16381 taaatataca acataccgca caatgtacct catagtgggg ctcaatatgt agtatatttt
    16441 tcgtagtata ttcatatagt tttaatcgtt tgactgtatt gttgttctaa tgtttaattt
    16501 aataagatat attaactaaa attctcgtcc aattacaaag tgcaattgac taccacttat
    16561 gcctgtacta ctcactgcat tgtct
//
LOCUS       DAOSRP010000027        16540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3595_length_16540_cov_5.361419, whole genome shotgun sequence.
ACCESSION   DAOSRP010000027 DAOSRP010000000
VERSION     DAOSRP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16540
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_16540_cov_5.361419"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="PLJ82_02895"
     CDS             complement(<1..1640)
                     /locus_tag="PLJ82_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR40489.1"
                     /translation="MQLSDRTIGGWLEYWAKETPNKECIVYSDRDLRFTWHDFDQRVN
                     DLAKGLMAIGIEKGHHVGIWATNVPDWLTFLFATSKIGAVLVTVNTNYKQHELEYLCQ
                     NSDMHTLCIIDGTWECDYVDMTYTMLPELKTSQRGYLNSERFPKMKNVVYIGQEKFRG
                     MYNTPELLLLGKNVDDKRLIDAKQNVHCHEVVNMQYTSGTTGFPKGVMLSHHNIVNNG
                     LITGENMKFTQDDKLCVCVPLFHCFGVVLAVMNCLTHGSTMVMVEKFDPLVVLASIHK
                     ERCTALYGVPTMFISELNHPMFSMFDMSSLRTGIMAGALCPIELMRQVSDKMFMTITS
                     VYGLTETSPGMTQTRLDDSFEVRCTTVGSDYPHVEVRVINPETGEECPVGVQGEMCCR
                     GFNIMKGYYKNQAATDEIIDKDGFLHSGDLGVKDENGNYRITGRIKDMIIRGGENIYP
                     REIEEFLYKMPEIKDIQVAAVPSKKYGEEVGAFIILKEGQTLKEEDVREFCRGKISRY
                     KIPKYVFFIDKYPMTGSGKIQKYKLKDMALEMCAQQGI"
     gene            complement(1690..2268)
                     /locus_tag="PLJ82_02900"
     CDS             complement(1690..2268)
                     /locus_tag="PLJ82_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOR40490.1"
                     /translation="MSQNNAFVGKKIKTIREGKDITVEQLAERSGLAVEQIRSIEEDE
                     KLPSLAPLTKIARALGVRLGTFLDDNTEIGPVVCRKGEKSGSLSFSNDMVGARKHMEY
                     YSLAGQKAGRHMEPFIIDIQPSVSKDFILSAHEGEEFIYVLSGEIEINYGKAVYHLQE
                     GDSIFYDSIVEHHVHAHKEKGAKILAVVYSPF"
     gene            2640..3623
                     /locus_tag="PLJ82_02905"
     CDS             2640..3623
                     /locus_tag="PLJ82_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOR40491.1"
                     /translation="MVVLLFTFFWGTRYGNYWVCFYNELYFLPIKIAATYFGLYYLIP
                     QLFKRNYYRVVVAGSFMLIGSGLLQRIIGYYSFIPEVGFYLPDIPLFDITEILHKIMD
                     INAVMIIPFSIKFYTHYLDKEHKILILSQEKTEAELQFLRSQIQPHFLFNVLNDIYAM
                     ALLNKKETPDSILKLADLMRYVLQESTASKVPLEKEINYIRNYIDLEKLRYNDLYAIS
                     FEIHGDSANKMITPLLLLPFIENAYKHSTTNDTGGAWIKIRISLTNEVLTLEIMNSFD
                     RNIPKVTQVSSGIGIQNVKKRLDILYQGKYTFYTENTPESYKTILTINLDT"
     gene            3620..4315
                     /locus_tag="PLJ82_02910"
     CDS             3620..4315
                     /locus_tag="PLJ82_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOR40492.1"
                     /translation="MKKIRCLLVDDEHLALEILELYCSKIDYLDVVGTCKNADEAAFF
                     LQHNKVDLLLLDIQMPRLNGMEWYKNLSSKPLVIFCTAFQHFAVESYEVNAIDYLLKP
                     FSLERFNKAIEKTKEIIEKKTNQETIEQSIFVRSDRKEIRVKIDDILFIQSMSNYYVV
                     TTTEKKIIGYGSISTLESTLQAFNFIRIHRSYLVAKNKIGTIATNSIIVSTYELPIGR
                     NYRKNIENIAKLS"
     gene            complement(4471..5166)
                     /locus_tag="PLJ82_02915"
     CDS             complement(4471..5166)
                     /locus_tag="PLJ82_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR40493.1"
                     /translation="MRKTHLLIGFLSIAIIFSGCKSWNNTAKGGLIGGGAGAAIGAGV
                     GILIGKNEKSAAIGAAVGTAVGATTGLIIGKQMDKAAEKAAAIEGAKVDTITDANNLK
                     AVKVTFESGILFASGKSILSTKSKTSLSDFAAILVENSTMDVAIMGHTDNTGSLELNK
                     KLSQERAQAVADFLVSKNVPTKQIKEVVGKDFSEPVADNATVEGKAANRRVEIYMYAS
                     EEMIKNAENAVKK"
     gene            5352..6629
                     /locus_tag="PLJ82_02920"
     CDS             5352..6629
                     /locus_tag="PLJ82_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOR40494.1"
                     /translation="MNKLAHCMFLAIFVTHTLYSCTTNEPTSPPQPKEEHITMNINKS
                     SPKAFYYSIDDALENAETPEKKLKLGDSIYLLAKEKQWIPIVFADTAVFLYHHSSTNN
                     ATVGVFGDLNGWSASESPQISLSRYADTRLYAGIYKAPSTTTRVDYKLVVNNAWILDP
                     GNPKLAWGGMGSNSEVALSEYEYSPWVKARSDINKGTLSANQLITSSALGYNINYRVY
                     LPYGYDSELSYPVLFVTDGHEYINNNLGALPNVADNMLHENKIEPLIIVFVDPRDPTN
                     GTNKRMTEYVANDNFVTFLADELYTTIAETYSIKNTPEHTAILGTSLGGLNSAYVGIK
                     RADVFGLLAIQSPAFWIHTDIFTLFNGDKQNLRIYMDTGTIHDTQDRALEMKTILENK
                     GYTFQYAEYSEGHSWGNWRARMDDVLLYFWGKE"
     gene            7314..11699
                     /locus_tag="PLJ82_02925"
     CDS             7314..11699
                     /locus_tag="PLJ82_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOR40495.1"
                     /translation="MKKFAIFLVIFVSSLFTSHLYAQSCPTQFSGTTFQPKTGVTSAN
                     YNMDQIRLGDPSSEVLTTGFAFKNVNTPDLNEYTISGNKASFRPKSGGNVGIFTYNLI
                     DLIPNKVYKIKINVTVRKIVGCSGDNTHRVNLMLKSGNNTLASINKENENFPNEFSQP
                     LEINFTPTTTKQSFSLVADHSSSCTYIDITSIEVIGCSSEKIVSENGEKVCAGNENQL
                     IAKGVTATSYTWQQSEDAGLTWTTIPQTTKVISVLPTTETRYKVTAGATTLPAITVRP
                     VVCCAVAGERAILAAERFTFSGTNLGARTDKTPTMISGNATTGTYLYASTGNIIEGRY
                     AVVKSTSVDGCTYWQDRGLTGNSKSLSTAAGTDKGTSDGFLLVNANVDPEVFYEQTFT
                     DLCPNTIYDFSAFVANATTENGQTRAPVNVGFRVFGCNGTDCTTQLIKVESGDLPSST
                     NWVEKGGSFNSQGFSSVKVQLFNNLDSKPYAIKEKDGTLKVIGNDIAIDDITFSTCSP
                     EIQVFSNAEYTKKDQTFLAENTERVYVWAPYDLTEFFATPWYVIQESLDGTSDWTNIY
                     SGPNDYVDVDITLDKYKDKKYNYRAFVAAYQKTAEERAANPKILKTGCGQISAVSDTL
                     WLTMEKPCTATPPPSVTNYDECPLTGTGTLATLVTSDTTQLRWYTQDTGGTALTTTTF
                     DWTTVGSTTLYVTNQLADQGTTTYCESTRTAVTINIKPSVAFTVDKTEINVCTNSTDA
                     ERTFTVINKFPATAAITWKAMSDESFLGTGNTYTVPATVGQGELIIDGTDPTGCDFRD
                     TVRYQVKALPDFTLTAPTMVCVSAPSATLRADITTDENGSTYAWYKGTSTTPVGSGTL
                     TNETSITHQDNSVGTTQGNVTYRLVINNGICQTPKTVTIAVGTEVNIPIISSATKNAI
                     CAGDLVDLTAQITLNTGETLLWKKDGVEMTETGTTITGQDLTANTVYTVELQGGSCDG
                     DGTITITVSTPITPELEISSDVICLGGTINIEDINTENAASYVWESRVGTGNWTTMSQ
                     TSKNLTGIAPEVTTTYRKTAINGECTRVSNEVTVRVEPPLNFDVSNDITICAGSTTTL
                     TMSNYPEEAVTFWIEGDINADDFMNAFKDAFVGETTNLLEALMNMMFSSLDDIVSDGN
                     GTENPVVEDDDLTNEDELPLIDESFFEEIFTSLGIIGMEPTIDVTPTTTTTYTAVVMG
                     VCTATRQVTVTVDAPITASISAPTTICVGDNTQLVATGGTTYRWTPATGLNNPNISNP
                     IASPSVTTVYSVEITRGTCSDVTETTVTVVPLPVITNIEETNVREVTAYVENGLPPYS
                     YSINNRDFITHNVLHDVSIGGHILYVVDMNNCKTSRPFEVTPIPIFPAKFFTPNDDGV
                     NDYWTIENLNVYPSYIVQIFDRFGKLIYEVRSGQFHADGEVNYHPFQWDGNYLGHPLP
                     STDYWYLITLEEVRKQYTGHFTLKR"
     gene            11781..12737
                     /locus_tag="PLJ82_02930"
     CDS             11781..12737
                     /locus_tag="PLJ82_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOR40496.1"
                     /translation="MKKYLVIAILSLTSIVSSSAQEEIVYNQYHFNYYLANPALAGAE
                     PCSHFMLTNKFQWVGMNEFPMTQTLSFRTRALDRIGIGAFLYNDRNGYSNRIGGQATF
                     AYHIPLSDGRRYRQNVSLDRQLSFGVSFKLNYFSINQELLDDPSTQGDNAFANPDGLA
                     PNANFGVYFKSYGFFTGLSLTNLIPTTPDIFGDKEVPAPFTGFFFLGNAFELDYDAFI
                     EPSIMLKANQYGNIGGDINLKFGQEVDRDFGYWIQASYRHSFDRDNGQSLMLMPMGGV
                     RFGKFQLGYSFGLDLNNLVTHSYGSHEIMLGYSFCIPKYFCR"
     gene            complement(13284..14171)
                     /locus_tag="PLJ82_02935"
     CDS             complement(13284..14171)
                     /locus_tag="PLJ82_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOR40497.1"
                     /translation="MLKAMFAHISAWKQFVVVVFLLLVAFFLSSTLFYVIASMGGVSQ
                     TFSSDINWLRFLQAFQSLMVFVLVPIVAGRMFSFNIKSYYIFRKPTTAQILWVIVLMS
                     SLIPLINISLQLNKLVVFPESMQSLYTWMLQLEQQATDVLQFFFSDTSVLGLVFSLFI
                     IAVIPAIGEELVFRGLLQKILTQCTKNAHIGIWISAFLFSFIHFQFFGFLPRMLLGAF
                     FGYLFLWSGNLLIPMVAHFVNNAIGVTMYWLYYRNSIDINIDTIGVEEHSMILTIFSI
                     IFVGLLFYFSPFRTKNPVT"
     gene            complement(14165..15067)
                     /locus_tag="PLJ82_02940"
     CDS             complement(14165..15067)
                     /locus_tag="PLJ82_02940"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOR40498.1"
                     /translation="MNTIPPFLKKGDTIAIISPSGVVDASFVYAAQEVLEKWGYKVVL
                     GKHCLAQDGRFAGTDSQRLSDLYWAFTNVDINAVLCSRGGYGLIRILSKLQETELFNQ
                     PKLLVGYSDITVLHNLLAKNSISSVHAPMAKHIAENPDDETSTFLCNTLAGVLPSFSI
                     PSHACNRNGNVKGKLLGGNLSVLYSLRGTPFDLDYTDSILVIEDIGERLYHLDRMLTN
                     FKLGGVFDKIKGLVVGQFSDMPSDELFPYSPYEIIQRAVAEYDFPVCCDFPIGHVQGN
                     YPVLLGAEYELSIQSTNTYLRPLC"
     gene            complement(15070..>16540)
                     /locus_tag="PLJ82_02945"
     CDS             complement(15070..>16540)
                     /locus_tag="PLJ82_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOR40499.1"
                     /translation="TVTTANSGHDALALCKKETYDIAFLDENMPGLSGLETLSEIKKM
                     QPALPIIMITKNEEEDIMEQAIGKKIADYLIKPVNPNQIVMAIKKCLHSKQLISDKVT
                     SSYQSAFTQIGMEINDTYTFEDWKKVYKHLVYWELELEHADNQMDSLLMMQKNEANAN
                     FTKYIRKNYTSWFEPNDDKPLMSPDIFKSKVFPFLDKGEKVCFVVIDNLRFDQWKVLQ
                     KELSQWFYTADDDLYCGILPTATQYARNAIFAGLMPLQIQKMFPHYWIEEEDEEGKNM
                     KEEELLQTQLDRFRKKYSFSYHKINTSQAGEKLNEQIHQYLANDLNVVVFNFIDMLSH
                     VRTESKIFRELVPDEAAYRSLILSWFKHSSTNDFFKILAEKGIKVVLTTDHGTIRAKN
                     PIKVVGDKNTNTNLRYKVGKNLSYNKKEVFPITQPELIGLPSPNISSHYIFATQEDFF
                     AYPNNYNYYVSYYKDTFQHGGISLEEMLIPIAVLEPKRK"
BASE COUNT         5480 a         3247 c         2749 g         5064 t
ORIGIN      
        1 tctatccctt gttgagcaca catctcaaga gccatgtctt tcaatttgta tttttgtatt
       61 ttaccacttc ctgtcattgg gtatttatca ataaaaaata catacttggg tattttatag
      121 cgagatattt tccctctaca aaattcgcgt acatcttctt ctttcagggt ttgcccttct
      181 ttcagaatga taaatgctcc tacttcctcg ccgtatttct tcgatggaac cgccgcaact
      241 tgaatatctt ttatctcggg cattttatac aaaaattctt ctatctcgcg aggatagata
      301 ttttcgcctc ctcgtataat catatcttta atacgacctg taatacggta attgccattt
      361 tcatctttta cacccaaatc gcccgaatgt aaaaacccat ctttatcaat tatctcgtca
      421 gtagcagctt ggtttttata ataccctttc atgatattaa accctctgca acacatttct
      481 ccttgtacgc ctacagggca ttcttctcct gtttctggat tgataacccg tacttcaaca
      541 tgaggataat cgcttccaac agtagtacaa cgcacttcaa acgaatcgtc taaacgtgtt
      601 tgtgtcatac caggtgatgt ttccgttaag ccatatacgc ttgtaattgt cataaacatt
      661 ttatcgctaa cctgcctcat tagttcgata gggcacaatg ctccagccat aattcctgtt
      721 cgtaacgaac tcatatcaaa cattgaaaac attgggtgat tgagttcaga gataaacatt
      781 gtaggcacac catacaaggc ggtacatctt tccttatgaa tagacgcgag cactactagc
      841 ggatcgaatt tttcgaccat taccatcgta gaaccatgcg ttaagcaatt cattactgcc
      901 aatactactc caaaacaatg aaacaatggt acacatacac ataatttatc gtcttgcgta
      961 aatttcatgt tttcacctgt aatcaagccg ttatttacaa tattatgatg cgaaagcatt
     1021 actcctttag gaaaaccagt tgtaccagac gtgtattgca tatttaccac ttcatgacaa
     1081 tgcacattct gttttgcgtc tattaatcgt ttatcgtcaa catttttacc tagcaacaac
     1141 aactctgggg tattgtacat ccctctaaat ttctcttgac ctatatacac cacatttttc
     1201 atcttaggaa aacgctcgct gttgaggtat cctctctgtg atgtttttaa ttcgggcaac
     1261 atggtataag tcatatccac atagtcgcat tcccaagtgc catcaataat acataaggta
     1321 tgcatgtcgg aattttgaca caaatactct aactcgtgtt gcttgtagtt tgtgttaacc
     1381 gttaccaata cggctcctat tttggatgta gcaaataaaa aagtgagcca atcaggcaca
     1441 ttggttgccc aaatgcccac atggtgccct ttttcaatgc ctattgccat taaacccttt
     1501 gccaagtcat ttactcgctg gtcaaaatca tgccaagtaa aacgtaagtc tctgtcagaa
     1561 tatacgatac attctttatt tggtgtttct ttagcccagt actcgagcca tcctccaatt
     1621 gttctgtctg ataattgcat atctataatt gctctaaaat tagtattcat aaagattcgt
     1681 tgaatggcac taaaaaggac tatacacgac tgctaaaatt ttagctcctt tttctttatg
     1741 tgcgtgtaca tgatgctcta caatagaatc gtagaaaata ctatctcctt cttgcaaatg
     1801 atacacagct tttccgtaat taatctcgat ttcaccagat aaaacataaa taaactcttc
     1861 cccttcgtgt gctgataaaa taaaatcttt gcttacagat ggttggatat ctataataaa
     1921 aggttccata tgacgaccag ctttttgacc tgccaaagaa tagtattcca tatgcttgcg
     1981 ggcgcctacc atatcgtttg aaaaacttaa acttccagat ttttcacctt tacgacatac
     2041 aactggacca atttccgtat tatcatccaa aaaagtacct aagcgaacac ctaatgcacg
     2101 tgctatctta gtcaatggag ctaatgaggg aagtttttcg tcttcttcta ttgaacgaat
     2161 ttgctctaca gctaaacccg aacgttcagc taattgttct acggtaatat ctttaccttc
     2221 tctaattgtt ttaatttttt taccaacaaa tgcattgttt tgtgacatag atttaatttt
     2281 ttatggttat cagattaaaa tatcatagta tatcgaccac aaaaatatac attttatctt
     2341 atcaaacaat atttttagct tatttacaac caaaagttat atttttgcgt taatcaagta
     2401 ttttttcaca tccaaataaa aacgaaaata gtccagaaga catattctaa aacttctaac
     2461 taacagagaa aaatacgttt acataaaaag aacttctatt tgcataaata taaaaagaaa
     2521 aatagagaga ggtagtacaa tttgcaaaaa aactgtatat ttgttcaact aaatcaatta
     2581 cttacatgcg aatgctaact gggaaaaatc gatggctact gcacattaca tattgggtaa
     2641 tggtggtgtt attatttaca tttttctggg gcactcgtta tggaaactat tgggtgtgtt
     2701 tctacaacga gttgtatttt ttacctatca aaattgcagc cacttacttt ggattatatt
     2761 atcttatacc acaacttttt aaacgcaact attaccgcgt tgtagttgct ggtagtttta
     2821 tgcttattgg cagtggtctt ttacaacgaa ttattggtta ctactcattt atccccgaag
     2881 taggatttta tttacccgac attcctctgt ttgacattac tgaaatttta cataagataa
     2941 tggatataaa tgcggtaatg attattcctt tttccatcaa attctacacc cattatttag
     3001 ataaagagca taaaatactt attttatctc aggaaaaaac ggaagcagag ctacaatttt
     3061 tacggagcca aatacagccc cattttttat tcaatgtgct taacgatatt tatgcaatgg
     3121 cattattgaa taaaaaagaa acacccgatt ccatcctaaa attagcggat ttgatgcggt
     3181 atgttttgca agaatccacg gctagtaaag tgccattaga aaaagaaatt aactatatcc
     3241 gaaattacat tgatttagaa aaattacgct ataatgattt gtatgctatt tcctttgaaa
     3301 tacacggaga tagtgcgaat aagatgatta cccctctttt gctactgcca tttatcgaaa
     3361 atgcttataa acactctact acaaatgata caggtggggc ttggataaag attcgtatca
     3421 gcctaacaaa tgaggtatta acgcttgaaa ttatgaacag ttttgaccgc aatatcccga
     3481 aagttacgca agtttcgtct ggtattggca ttcaaaatgt aaaaaaacga ttggatattt
     3541 tatatcaggg taagtacact ttttacacag aaaacactcc tgagagttat aaaactatac
     3601 taacaataaa cctagataca tgaaaaagat tcgttgttta ttggtagatg atgagcattt
     3661 ggctttagag atactggagc tatattgcag taaaattgat tatcttgatg tagttggcac
     3721 gtgtaaaaat gccgatgaag ccgcattttt tttgcaacac aataaggttg atttgctatt
     3781 attagatatt caaatgccaa gactaaacgg aatggagtgg tataaaaatc tatcaagcaa
     3841 acctcttgtt atattttgta cagcctttca gcattttgcc gtagaaagtt acgaagtaaa
     3901 tgctattgat tatttactta aaccattttc attggaacgc tttaacaaag caatagaaaa
     3961 gacaaaggaa ataattgaga aaaaaacgaa tcaagaaacc atagaacagt ctatttttgt
     4021 tcgttctgac agaaaagaaa ttcgggttaa aattgacgat atactattta ttcaaagtat
     4081 gagcaactac tatgtagtaa ccacgacaga gaaaaaaata attggatacg gttctatttc
     4141 aacactggag tcaactcttc aagcttttaa ttttatacgc attcatcggt catatttagt
     4201 agctaaaaat aaaattggaa ccattgctac aaactctatt attgtatcta cttacgaact
     4261 gccaattggt cgcaattatc gaaaaaatat agaaaatatt gctaaattat cataactatg
     4321 caaaacaaaa tatagaactc gaaacaagct tatctttaga cccttaccag tattatattt
     4381 tacaacgcac tgtaaactga aactacttat actaatacaa aaaaaagagt ttgccataca
     4441 gcaaactctt tctccctaaa caaaagaatg ttattttttt actgcatttt ctgcattttt
     4501 aatcatctct tcgctcgcat acatatagat ttctacacga cgattcgctg ctttaccttc
     4561 tacagtagca ttatcagcta ctggctcaga aaaatcttta ccaactactt ctttgatttg
     4621 ttttgttgga acattttttg acaccaaaaa atcagctaca gcttgtgctc tttcttgcga
     4681 taatttttta ttcaactcca atgaaccagt attgtctgta tgtcccataa tagctacatc
     4741 catagtagaa ttttctacta aaatagcagc aaaatcactc aatgaggttt ttgatttagt
     4801 actaagaata cttttgccac tagcaaacaa aatacccgat tcaaacgtaa cttttactgc
     4861 ttttaagtta ttagcatcgg taatcgtgtc tactttagca ccctcaatag ctgcggcttt
     4921 ttctgcagct ttgtccattt gtttcccaat aataagccct gtagtagctc ctactgctgt
     4981 acctaccgct gcaccaattg ctgcactttt ctcattttta ccaatcaaaa taccaactcc
     5041 tgcacctatt gcagcaccag cacctcctcc aatcaatcca cctttagcgg tattgttcca
     5101 cgatttgcat cccgaaaaaa tgatagcaat actcaaaaat ccaataagta aatgtgtttt
     5161 tctcattttg ttaaacgttt tttgtaaatt acttgtttta ttaaaatctg cacaaagata
     5221 caaagagtag ctatatctaa aaaattttat gtgacaaaaa atctcaaaaa gatactttta
     5281 tgaatatatt tggtttatca aaatatattc cttatttttg aaacataaac gaactttaca
     5341 ttaagtgtac tatgaacaaa ttagctcatt gtatgtttct tgccattttt gttactcata
     5401 ccctgtattc gtgtactaca aacgaaccaa catctccccc tcaaccaaaa gaggaacata
     5461 taaccatgaa tatcaataag tctagcccaa aagcatttta ttattctatc gacgatgctt
     5521 tagaaaacgc tgaaacaccc gaaaaaaaac tgaaacttgg cgatagcatt tacttgcttg
     5581 ccaaagaaaa acaatggata ccgattgtgt ttgctgatac ggctgtgttt ttgtatcacc
     5641 attcgagtac aaacaacgcc acagtgggag tttttggcga tttaaatggt tggagtgcct
     5701 ctgaatcccc tcaaatatca ctttctcgct atgctgatac tcgcctatac gctggcattt
     5761 ataaagcccc ctctactact acccgcgttg attataaact agttgtaaac aacgcatgga
     5821 tactagaccc aggcaaccct aaattagctt ggggcggaat gggttccaat tcagaagtag
     5881 cccttagcga atacgaatat tctccttggg taaaagcacg ttcggatata aacaaaggaa
     5941 ctctttctgc taaccaactg attaccagtt cagctttggg gtacaacatc aactaccgtg
     6001 tatatttgcc ttatggatac gattcggagt tgtcttaccc cgttcttttt gttacagacg
     6061 gacatgaata cataaacaat aatttgggtg cattacctaa tgtagctgac aatatgctcc
     6121 acgaaaataa aatagaacct cttattattg tatttgttga tccacgcgac ccaaccaacg
     6181 gcacgaacaa acgaatgact gaatatgttg ccaatgataa ttttgtaaca ttccttgcag
     6241 atgaattata tactaccatt gctgaaacat atagcattaa aaatactcct gaacacaccg
     6301 ccatactagg cacttcgttg ggtgggctta atagtgctta tgttggcatt aaacgtgctg
     6361 atgtatttgg attactagcc atacagtcgc ctgcattttg gattcacacc gatattttta
     6421 cgctattcaa cggcgataaa cagaacttac gtatttatat ggatactgga actatccacg
     6481 acacgcaaga tagggcatta gagatgaaga caattcttga aaataaaggc tatacatttc
     6541 agtatgccga atacagcgaa ggacattcgt ggggcaattg gcgagcacga atggatgatg
     6601 tgttgctgta tttttggggt aaagagtagg agttaaatta gttgaaagtt gaaagttgag
     6661 agttgagagt ttggaaccct gtgtactatt tctcacacga agaacaccaa gataacacga
     6721 agttacacga agaaaaaact aaatcctctg tgcccctctg tgattcctcc gtaaaactct
     6781 gtgaaagatt ctcacacgaa gaacacgaag ataacacgaa gttacacgaa gaaaaaacta
     6841 aatcctctgt gcccctctgt gattcctccg tagaactctg tgtaagattc tcacacgaag
     6901 aacacgaaga catctattcc gctccgctgg agctaataac ttaatattgg catctttctt
     6961 ttctattatg atgtagcccc tacggggcat gaaaagttgc atttttcctt aaaactggca
     7021 aaacaccagc tccgtaggag cgacataata atatgctgat agcaatctat ttttaaaatt
     7081 ataaactctg tgtaacaatt attaacaaaa ccgtgcaaga acctaacaaa ataccgcaaa
     7141 taaggaatca acaaacacta atcactaagc attctcctcc ccttacacaa taaaaatagc
     7201 atttttttag ttaaatactt aactattttt tttatttcag aatagatttt gtatttttga
     7261 gggctttttg tgtgaataag ccttaataac cttatattta atatagactt gctatgaaaa
     7321 agtttgctat ttttctcgtt atttttgtat cctctttgtt tacctctcat ctgtatgccc
     7381 aaagttgtcc aactcagttt agtggcacca cgtttcaacc aaaaacaggg gtaacttctg
     7441 ccaattacaa catggatcaa atccgtttag gagatccatc ctcagaagtt ttaaccactg
     7501 gttttgcttt taaaaatgtg aatacgcctg atttaaacga atatacaata agtggaaata
     7561 aagcctcttt tcgtccaaaa tcaggaggca acgttggtat ctttacttat aatttaattg
     7621 atttaatacc taataaggta tataaaatta aaataaatgt tactgtaaga aaaattgttg
     7681 gttgctcagg agacaatact catcgggtaa atttaatgct aaaatcaggg aacaatactc
     7741 ttgcatcaat aaataaagaa aatgagaatt tcccaaacga attctctcaa ccattagaaa
     7801 taaatttcac tcccacaaca acaaaacaat cgttctctct ggttgccgat cattcaagtt
     7861 catgtaccta tatagatata acctctattg aagttatcgg atgctcttct gaaaaaattg
     7921 tatcagaaaa cggagaaaaa gtatgtgcag gtaacgaaaa tcaattaatt gcaaaaggtg
     7981 ttactgctac ttcttacaca tggcaacaat ctgaagatgc gggtctaact tggacaacca
     8041 tccctcaaac aacgaaagtt atctcagtat tgcctactac cgaaacacgc tacaaagtaa
     8101 cagcgggtgc caccacacta ccagctataa cagtccgtcc agtagtatgt tgtgcagtag
     8161 cgggtgagag agcaatcctt gcagcagagc gatttacttt ttcaggcaca aatttaggag
     8221 cccgtacaga caaaacacca accatgatat cgggaaacgc tacaactggt acctatctat
     8281 atgcaagtac aggaaatatt attgaaggtc gatatgctgt tgttaagagc accagtgttg
     8341 atggatgtac ctattggcaa gatagaggtt taacaggtaa ttcaaaatcg ctatctactg
     8401 cagcaggaac agacaaagga acctcagatg gatttttgtt ggttaatgct aatgtggatc
     8461 ctgaggtatt ctacgaacaa acatttacgg atttatgtcc caacacgatt tacgactttt
     8521 ctgctttcgt tgcgaatgct acaaccgaga acggacaaac aagagcccct gttaatgttg
     8581 gattcagggt atttggctgt aatggaaccg attgtactac acaattaata aaggttgaat
     8641 caggagactt accctcaagc accaactggg tagaaaaagg agggtcgttt aattctcaag
     8701 gattttccag tgtaaaagta caactattca acaacttaga ctctaaaccg tatgctataa
     8761 aagagaagga tggtacatta aaggttattg gtaatgacat tgccattgac gatatcacct
     8821 ttagtacctg ctctcctgaa atacaagtat tctcaaatgc agaatatact aaaaaggatc
     8881 aaacattttt agcagaaaat acagagaggg tatatgtatg ggctccttac gaccttaccg
     8941 agtttttcgc aactccttgg tatgtaatac aagagtctct cgatggaaca agtgattgga
     9001 caaatattta tagcgggccc aacgactatg tggatgtaga tatcacacta gataagtata
     9061 aagataaaaa atataactat agagcatttg tagcagctta ccaaaaaaca gcagaagaaa
     9121 gggcagctaa tcctaagatt cttaaaactg gttgcggaca aatttctgca gtttctgaca
     9181 cattatggtt aacaatggaa aagccttgta cggcaacacc tcctccatca gtaactaatt
     9241 acgacgaatg cccattaact ggaacaggta ccttagccac tttggtaacc agtgatacaa
     9301 cacaacttag atggtacaca caagatactg gagggacagc acttactact accacatttg
     9361 attggacaac agttggctct accactcttt atgtaacaaa ccaactggca gatcaaggca
     9421 ctacgactta ttgcgaaagt acacgtacag ctgttactat caacattaaa ccaagtgttg
     9481 catttacggt agataaaacg gaaattaatg tgtgtaccaa ttcaacagat gctgaacgca
     9541 cctttactgt aatcaataaa tttccagcta cagccgctat cacatggaaa gcaatgagtg
     9601 atgaatcatt tttaggaaca ggcaatacgt atactgtacc tgctactgtt ggtcaaggcg
     9661 aactaatcat agatggcact gaccctactg gttgtgactt cagagataca gtgaggtacc
     9721 aagtcaaagc tttgcctgat tttacgctta ctgctcctac catggtgtgt gtgagtgctc
     9781 catcagctac actaagagct gatattacta ccgacgaaaa tggaagtacc tatgcctggt
     9841 acaaggggac tagtactacc ccagttggct ctggaacatt aacaaatgaa acaagcatta
     9901 cacatcaaga caactctgtt ggcacgactc aaggtaatgt tacataccgc ttggtaatta
     9961 ataatggtat ttgccaaaca ccaaaaacag taaccatagc agttggtaca gaagtaaata
    10021 taccaattat ctcttctgct accaaaaatg caatttgcgc tggcgattta gtagatttaa
    10081 ctgcccaaat taccctaaac actggcgaaa cattattgtg gaaaaaagat ggtgtagaga
    10141 tgacagaaac aggaacaacg attactggac aagatttaac tgcaaataca gtgtatacag
    10201 tagaactaca aggaggctct tgtgatggag atggtactat tacaattacc gttagtactc
    10261 ctataacacc cgaattagaa atttcgagtg atgttatctg cttaggagga acgataaata
    10321 tagaagatat caacacagaa aatgctgcta gttatgtgtg ggagtctcgt gtaggaacag
    10381 gcaactggac aaccatgagc caaacaagca aaaacttaac cggcattgca cctgaagtaa
    10441 ctacgactta tcgcaaaaca gctattaatg gagaatgtac acgtgtgagc aatgaagtta
    10501 ctgtgagagt tgaacctcct ttgaattttg atgtaagtaa cgacattaca atttgtgcag
    10561 gttctactac aactcttact atgagtaatt atccagaaga agctgttaca ttttggattg
    10621 aaggagacat aaatgctgat gattttatga atgcatttaa agatgctttc gtgggtgaaa
    10681 cgactaacct tttagaggct ttgatgaata tgatgttctc atcattagat gacatagttt
    10741 ctgatggaaa tggcacagag aacccagttg tagaggatga tgaccttaca aatgaagatg
    10801 aattaccttt aatagatgaa agcttttttg aagagatatt tactagctta ggtattattg
    10861 gaatggaacc tacgatagac gtaacaccaa ccaccaccac tacttatact gctgtagtta
    10921 tgggagtatg tacagcaacg cgtcaagtaa cagtaacagt cgacgctcca attacagcaa
    10981 gcatatctgc ccctactacc atctgtgtgg gagacaacac gcaactcgtt gctactggag
    11041 gtacaaccta tcgctggact ccagctactg gattaaacaa tccaaatata agcaatccaa
    11101 tagcatcgcc aagtgttact actgtatatt cggtagaaat tacacgagga acttgctctg
    11161 atgtaacaga aacgacagta actgttgttc ctcttcctgt aattaccaac attgaagaaa
    11221 ccaatgtgag agaagtaact gcttatgtgg aaaatggctt acctccttat tcctattcta
    11281 ttaacaatag agatttcata acacataatg tgctacatga tgtaagcata ggtgggcaca
    11341 tactatatgt tgttgatatg aataattgca aaacatcaag gccgtttgaa gtaacaccta
    11401 ttccaatatt cccagcaaaa ttctttacac ccaacgatga tggtgtaaat gattattgga
    11461 caatagagaa cttaaacgtt tatccatcgt atattgtaca aatatttgac cgttttggca
    11521 agttaattta cgaagtgcgt tcaggacaat tccatgcaga tggcgaagtt aattatcatc
    11581 cattccaatg ggacggtaat tatcttggac atccattacc aagcacagac tattggtact
    11641 taattacttt agaagaagta agaaaacaat acacgggaca ttttacgcta aaaagataat
    11701 agaaatatca atgaaaattg tatctttggc acataaatat tttttagaaa acgaaagaga
    11761 cctattttta atctgaacat atgaaaaaat atctagttat tgcgattcta agtttgacaa
    11821 gtattgtatc ctcgtcagca caagaagaaa tcgtgtacaa ccaataccat tttaattatt
    11881 atttagctaa tcccgcattg gctggtgctg agccttgttc acattttatg ctaacaaata
    11941 aatttcaatg ggtagggatg aatgaattcc ctatgacaca aacattaagt ttccgtacta
    12001 gggcattgga cagaatagga ataggggcat ttttgtataa tgaccgtaat ggatattcaa
    12061 atagaatagg agggcaagct acatttgcct atcacatccc attaagtgac ggtcgtagat
    12121 atcgtcaaaa tgtatcccta gatagacagc tatcatttgg ggtatcattt aaattgaatt
    12181 attttagcat taaccaagaa ttattggacg accctagcac acaaggggat aatgcttttg
    12241 ccaatcctga tggacttgcg ccaaatgcta attttggtgt ttactttaaa tcatatggtt
    12301 tctttactgg tctatctctt acaaatttaa tcccaacaac acccgatatt tttggtgata
    12361 aagaagtgcc tgctccattt acaggattct ttttcttagg aaatgccttt gaactagatt
    12421 atgatgcttt catcgaacca tctattatgc taaaggcaaa tcaatatggg aatattggag
    12481 gggatatcaa tttaaaattt ggtcaagaag tagatagaga ttttggttat tggattcaag
    12541 catcgtatcg tcacagtttc gaccgcgata atggacaaag tcttatgcta atgccaatgg
    12601 gtggtgtacg tttcggcaaa ttccaattag gatactcatt tggcctagac ttaaataact
    12661 tggtaacgca tagctacggt tcgcacgaaa taatgcttgg atattctttc tgtattccaa
    12721 aatatttctg tagataagaa aatactgata caccaaagcc ccgattactg caaaaagttt
    12781 tcggggcttt ttttaatcaa gtctctgtgt aaaaattctt tcacgaagat acacgaagta
    12841 aaaaatttaa acctccgtgc cactctgtga atcctctgtg gaacttcgtg taaaaatctt
    12901 tgacgaagaa cacgaacaaa gatttacaac tcagtacgat aactataacc tagagaaaat
    12961 caaacaaaga tatctattcc gctccgctgg agctctcttt atattgatat gttttttcta
    13021 ttaatatgta gcccctaacg gggcatgaga aatagcgatt tttgcttgaa aataagtaaa
    13081 acccagctcc agcggagcga aataataata gcataagcac agattgggta atgcaaactc
    13141 cgtaggagca acatattacc acgcaaatag cgttatattt ttaataaatt acctatctct
    13201 gtgaatcctc tgtgtctatc tgtgtaatgt ttttgttacg aaaatacacg aaaaaaaatt
    13261 ttaaactctg tggaataata tttctacgta actggatttt tcgtgcgaaa aggagagaaa
    13321 taaaacaaca aaccaacaaa gataatcgag aatattgtta gtatcatact gtgctcttct
    13381 acaccaatag tatcaatatt tatatctatg ctatttctat aatacaacca atacattgtt
    13441 accccaatag cattatttac aaaatgagcc accataggaa ttagcaaatt tccactccat
    13501 aggaataaat agccaaaaaa ggctcccaat aacatacggg gcaaaaatcc aaaaaactga
    13561 aaatgaataa aactaaacaa aaacgcactt atccaaatac caatatgagc attttttgtg
    13621 cactgcgtaa gaattttttg caataagcct ctaaacacta attcttcgcc aatagctgga
    13681 atgaccgcaa taataaacaa agagaatacc aatccaagaa cactggtgtc agagaagaaa
    13741 aactgcaaca cgtccgtcgc ttgttgttct aactgcaaca tccacgtata taatgactgc
    13801 attgattctg gaaaaacaac taatttgttg agttgcaaac taatattaat aagcggtata
    13861 agcgaactca tcaacacaat aacccataag atttgagcag tagtaggctt ccgaaaaata
    13921 taatatgatt ttatattgaa gctaaacatt ctccctgcaa caatcggtac aagcacaaaa
    13981 accatcaaag actgaaaggc ttgcaaaaat cgtaaccagt taatgtccga cgaaaaagtc
    14041 tgactaacac ctcccatgct agctatcaca taaaataatg tagaactaag aaaaaaagca
    14101 actaaaagta gaaacaccac caccacaaat tgtttccaag cagaaatatg agcaaacatt
    14161 gcttttaaca taacggacgc aaatatgtgt tagttgattg aatagataat tcgtactcag
    14221 cacccaataa tacagggtaa ttaccttgta catgaccaat aggaaaatcg caacatacag
    14281 gaaaatcata ctccgctact gcccgttgaa tgatttcgta tggagaatat gggaacaact
    14341 catcgctcgg catatcggaa aactgaccca caaccaagcc tttaatttta tcaaaaacac
    14401 ctcctagctt gaaattagta agcattctat ccaaatgata caaacgctcg ccaatatctt
    14461 caattactaa aatgctatca gtataatcca aatcgaatgg tgtaccacgc aaactataga
    14521 gcactgacaa gtttcctcca agcaattttc ccttcacatt gccatttctg ttacatgcat
    14581 gagatggaat tgaaaacgat ggcaagactc ctgcgagcgt attacacagg aatgtactcg
    14641 tctcatcatc tggattttca gctatatgtt tcgccatagg tgcatgcacc gaactgatac
    14701 tattctttgc caacagattg tgtagaacgg taatatcgct atatcctact aataattttg
    14761 gttggttaaa caattccgtc tcttgcaact tcgacaagat acgaatcagt ccatatccac
    14821 ctctactgca taaaacagcg ttaatatcta cattggtaaa agcccaataa aggtcagata
    14881 agcgttggct atctgttcca gcaaaacgac cgtcctgagc taaacaatgc ttaccaagaa
    14941 ccactttgta tccccacttt tctaacacct cttgagcagc atacacgaaa ctagcgtcca
    15001 caacacctga tggggatata atagcaatag tatcgccttt ttttaaaaat ggaggaatcg
    15061 tgttcatcgt tattttcgtt taggttctaa taccgcaatt ggaatgagca tttcttccaa
    15121 agaaatacca ccatgttgaa acgtatcttt gtaataactg acgtaataat tatagttatt
    15181 cggataggca aaaaaatctt cttgcgtagc aaaaatataa tgagaactga tattgggact
    15241 aggcaaccca atgagttctg gttgggttat aggaaatacc tcttttttgt tgtagcttaa
    15301 attttttcct actttgtagc gtaaattcgt atttgtattc ttatctccta ccactttaat
    15361 cggatttttt gcacgtatag taccatggtc ggtcgtaagg actactttaa tacccttttc
    15421 tgctaatatt ttaaaaaaat cattggtaga agaatgtttg aaccacgata aaataagcga
    15481 ccgatatgca gcctcatcgg gcaccaactc tctaaaaatt ttggattcgg tacgcacatg
    15541 cgataacata tcaataaaat taaataccac cacattcaaa tcatttgcca aatactgatg
    15601 aatctgttcg ttcaatttct ccccagcttg cgaagtatta atcttatgat acgaaaaact
    15661 gtattttttt ctaaatctat caagttgcgt ttgtaacaac tcttcttcct tcatattttt
    15721 cccctcctca tcctcctctt ctatccaata gtggggaaac attttttgaa tctgcaaagg
    15781 cattaatccc gcaaaaattg catttcgtgc atattgagta gcagtaggta aaataccaca
    15841 atacaaatca tcatctgcag tgtaaaacca ttgagacaat tctttttgca atactttcca
    15901 ctgatcgaaa cgcaaattat caatgaccac aaagcacacc ttttctcctt tgtctaggaa
    15961 tggaaaaacc ttcgatttaa aaatatctgg actcatcaaa ggtttatcgt cattgggttc
    16021 gaaccacgat gtgtaattct ttctaatata ctttgtaaaa tttgcattgg cttcattctt
    16081 ttgcatcatc agcaacgaat ccatctgatt atctgcatgc tctagctcca actcccaata
    16141 cactaaatgt ttgtaaacct ttttccaatc ttcgaatgta tacgtatcat ttatctccat
    16201 accgatttgg gtaaaagcag actggtatga actcgttact ttatctgaaa ttaattgttt
    16261 ggaatgcaaa cattttttaa ttgccatcac aatttggttg ggattaacag gctttattaa
    16321 gtaatctgca atttttttcc caattgcctg ctccataata tcctcttcct catttttcgt
    16381 tatcatgata ataggcaatg ctggttgcat tttttttatc tctgacaagg tttcaagacc
    16441 gcttaatcct ggcatatttt cgtctaagaa agcaatatcg tacgtttctt ttttgcacaa
    16501 tgccaatgca tcatgtccac tattagccgt agttaccgta
//
LOCUS       DAOSRP010000028        16484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3612_length_16484_cov_5.120945, whole genome shotgun sequence.
ACCESSION   DAOSRP010000028 DAOSRP010000000
VERSION     DAOSRP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3612_length_16484_cov_5.120945"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLJ82_02950"
     CDS             complement(<1..202)
                     /locus_tag="PLJ82_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40500.1"
                     /translation="MSNITQNIKSYKVVYPQIYSYTLPELKPNEGSQKIGYTEQENVH
                     DRILQQVKTAAIRLKYELLWSAP"
     gene            complement(610..1503)
                     /locus_tag="PLJ82_02955"
     CDS             complement(610..1503)
                     /locus_tag="PLJ82_02955"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOR40501.1"
                     /translation="MPPIDDALVCIISPIHGFNYPKITLDFIRNFPKGNNRVVLMNTR
                     AGMKIGNFVTPGLTGIAFFVSSFILRNKGYKIVGQIPFDMPSNWLSIHPALNEKTVKY
                     LHRTNYNRVEKHAVNIFSGQRDFLAYRDVIQDILISPIAIAYYFIGRFILAKSFYATI
                     DCDSCGLCIKQCPVQAINLFHKHPFWTFKCESCMRCMNNCPKRAIETAHGLLIGVSIV
                     SSVAFTIVVERVLQLEIHSSFIRFTLSSVIFIAILWILYYIQHMLLKYRILSKIVSLL
                     SLTHYTCWGRYKSIPDKKWKE"
     gene            1472..1615
                     /locus_tag="PLJ82_02960"
     CDS             1472..1615
                     /locus_tag="PLJ82_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40502.1"
                     /translation="MHTKASSIGGIFLSHFAMSTISQSILFLIANSENYKAMRFALPV
                     PEK"
     gene            1852..2346
                     /locus_tag="PLJ82_02965"
     CDS             1852..2346
                     /locus_tag="PLJ82_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOR40503.1"
                     /translation="MNKSIWLLAFVVLPTWLFAQQTLNEKLILQLVNEIRTEGCYCGN
                     EEVKPVNEVLWNDKLASIATQHSHDLFTYTSKNENKFVYLSHISTDGSTLDDRLKKAN
                     VTANNIRENIGYVQGNERIIVDYWINNPESCKNLMHPTAASIGIGRSGNFWTMLLTSA
                     FITK"
     gene            2404..3663
                     /locus_tag="PLJ82_02970"
     CDS             2404..3663
                     /locus_tag="PLJ82_02970"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOR40504.1"
                     /translation="MQTIDNFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAMPTLK
                     KILADGGSVIIASHMGRPKKNPDAKYSLQYILKHVEKLLGVPVKFAADCAKASDMAAS
                     LKNGEALLLENLRFYAEEEGKARGLAEDATDEEKAIAKKAIKESQKEFTKTLASYADC
                     YVNDAFGTAHRAHASTALIAKHFDENNKMFGYLMEKEVLAVQKILSDIERPFTAIMGG
                     SKVSSKIEIIENLLGKVDNLILAGGMTYTFSKAMGGNIGISICESDKLDLALELIEKA
                     KKNNVNLVLAVDAKIGDDFSNDCNTQFVSVKEIPDGWEGLDIGPETEKIFAEVIKNSK
                     TILWNGPTGVFEFENFTSGSKAVANAIVEATKKGAFSLVGGGDSVACINKFGLADQVS
                     YVSTGGGALLEAIEGKVLPGIEAIRGF"
     gene            complement(3719..4168)
                     /locus_tag="PLJ82_02975"
     CDS             complement(3719..4168)
                     /locus_tag="PLJ82_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOR40505.1"
                     /translation="MLEFELPRYTFRVKKQAEKTYILDAYRAKYVRATPEELVRQQMC
                     AYLQNERAYPKTMIFNECVLRINTMNKRCDTVIYSTAHTPIMLIEYKSPDIQISQAVF
                     DQVMLYNRVLQVPYILISNGKQHFCCKLDTDLQQYIFLNEIPVYNNL"
     gene            4205..4978
                     /locus_tag="PLJ82_02980"
     CDS             4205..4978
                     /locus_tag="PLJ82_02980"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOR40506.1"
                     /translation="MKTKQEIIQNWLPRYTERPLKDFTDLILLTNFNNYVDIFAEWYN
                     VPVLAKNGMMRNASAEGITIINFGMGSPNAALIMDILPAIKPKAVLFLGKCGGLRDKN
                     RLGDYILPSAAIRGEGTSNDYFPPEIPALPAFSLQRAVSSNIRNMEKDYWTGTVYTTN
                     RRVWEHDEAFRAYLMKTRAMGIDMETATLFSAGFYNGIPIGALLLVSDMPLQKDGIKT
                     SDSDKHVTANFVEEHLLIGIKSLSSIKRQSNTIKHLRYE"
     gene            4990..6006
                     /gene="holA"
                     /locus_tag="PLJ82_02985"
     CDS             4990..6006
                     /gene="holA"
                     /locus_tag="PLJ82_02985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR40507.1"
                     /translation="MAKKTLTYTEIITSLQAKKFAPIYLLMGEEPYYIDLISDYIEKN
                     ILTEDEKMMNQVVRYGKDSDVGSIINEARRFPMMATHQVIIVKEAQMLDKIEQLEMYL
                     QKPLETTILVINHKYKSADKRKKWVTLAEQCGVVFESNKLRDYQMPAMIEQMAREKGM
                     QINEKAKAMLTDFVGTDLAQMAGVIDKLRIILPKENNQITPEIIELNIGISKDFNAFE
                     LTNALIRRDVLTANRIVHYFSQNEKEHPIQATLAVLFNFFSNLMCFHFLPNQQDDYVA
                     KELKIHSFFVKDYKYAAKAYNKTQVFKIISLLREYDAKSKGIKNSSASGGELLKELVY
                     KIMH"
     gene            complement(6003..6680)
                     /locus_tag="PLJ82_02990"
     CDS             complement(6003..6680)
                     /locus_tag="PLJ82_02990"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOR40508.1"
                     /translation="MTFLPYNQDVDATVKEIMIKIRVAMNGVTSELMSDRGVKYKTNF
                     GVAFPDLQKIAQQYSPNELVATYLWHKSVRETKILAILLYPAQALTLEKSITWISDCD
                     TVELIEYLCAHLVVFTPFVPTLITQLARISEEKQIIAAYTLAAHYCKRNKPELTEEFS
                     PLLSFSSIHITAKLAQSMLYFLLYAYQKNEFKENIINEMQNLKKNQSENHVAMWLYTE
                     FEALSQD"
     gene            6809..6884
                     /locus_tag="PLJ82_02995"
     tRNA            6809..6884
                     /locus_tag="PLJ82_02995"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6844..6846,aa:His,seq:gtg)
     gene            7178..8323
                     /locus_tag="PLJ82_03000"
     CDS             7178..8323
                     /locus_tag="PLJ82_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR40509.1"
                     /translation="MLQRYLDTYWVNLLLLKSVVISVLFISSCSTEEPTPKPSTLCNI
                     SEISFESEQSAMLITVNTKESWSIEHLSNWIDFTATTGRGKTSLLVGATKNDGFGRID
                     SFLIKTAKQTHKIYIEQKGAAIVHLDILSHTLVFVKVEGGTFKIGNYQNPHEHDVSLT
                     EYYIGQTEVTNAFWEAIVGSLPYSNENKNYPYTMDDDNPDFPVTAVSWNDIHTTFLPL
                     LHTKTSHTFRLPTEAEWEFAALGGNKSKGYTFSGSNILDVVGWDASNANDTKQAVAQL
                     EPNEINLYDMSGNAAEWCSDWYSDWYNTTEDTTNPTGPVSGTYKVIRGGSIASEAGIL
                     GFHPCHAKQRSYAHPSGFQGAWGDTGHPDEPVIYKCNEIGFRLVLVR"
     gene            8453..8989
                     /locus_tag="PLJ82_03005"
     CDS             8453..8989
                     /locus_tag="PLJ82_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40510.1"
                     /translation="MELPKQTEKAFAVKTLFNEQLPTGDLVVLGALGYEIEGGATPYE
                     LAWYENNQLIAKGNIVAFKPQESAQYSLVVKDKNNCSKTLSINVDALKRAINDTQKPS
                     NFVSLSPTLVKDHIMLRCHSEPIASVHLKFIDINGNTCIETSITGSTYLPVSLASGLH
                     IVLIQYGDNVWVEKIMVE"
     gene            9011..14452
                     /locus_tag="PLJ82_03010"
     CDS             9011..14452
                     /locus_tag="PLJ82_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOR40511.1"
                     /translation="MIHKKIYTLFVILLTLTANSYGNSDAIHKVADTFFNFTKDGKRK
                     AVAETKPIEVKYVSINNNKSNVFVVQQDGEGFAIISQHGDASSVVAYIPNGDFEKAKL
                     SPSFMSVLQIYENNNPETTPPLHTKWDVLGTTQPLLDQAGVSLNQFYHASLGNCPTGC
                     VATALAQIMAYHKFPSTGKGSHCYTHPQYGQQCADFENTHYNWTNPTYDDYELLSYHI
                     GVATEMNYCGSKYGSSPSRSNYSEVLELYYGYSMRKGTRDMKYLKTEIDEMRPVYLEL
                     GGDPGHAVVIDGYKDENYLHLNFGWGGQANGYYMMNTNSTFWVGYTFGTNLGSNIFYS
                     PTPFITNESDSLALIAIHNALDGTTGWNTSLPVSKWHGVITHHSRVVELGLGGYDYGV
                     KGNIAPEIALLNKLQHLTIIGSELNGNLTADIFQLPDLESLHISKNAGTLQCEFPSQI
                     DGCQKLTHLSMPGILTGTIPESIGNLVQLQELSIYNGTLSGSIPLTISQLTKLKTINI
                     SKNQLSGPIPDAFYNLVDVETLDLSENNLSGNLPISLEKMTQLKELRLNKNNLSGILS
                     DVFEHVKNISYLDVSENHLSGNVPESIGKLDKLQKLFLHNNQFVALPNDMSGLEALNE
                     LLVNNNLLTTLPLSINAISSLRTIDASNNQITTLHRDFGLLRNLQSLNISNNQLTEIP
                     SSIFLNAKLGTILLSNNKIKAFPSSIDFLSSSIDFAKFDDNELEGQIPLNLLSAKATH
                     VSLINNRFTYEDVPVSDELRHAVGLQKNHRLKQNKFPVMMGDTIRIDIRKISKLSHPD
                     NLYYWLEYPKYADKNGIAFLEQLQPNPILTVVINEKTAAKKFYCKVFNNNVPTWTFFF
                     NNYEYINPTMSVVNTDTIEFEILSEDEMMAYIHAESNFIQSKQILNKTISDHMVTLVP
                     PLTTYRGSLVWQASTDGIAWHSITNTMANETIKGNIVSIKKDSLVISPLADAFYRTAI
                     VESDCNPLYSDTVHIKAYGKLLYDKEINTSNDDVKIELDDIEVLVPKGIHNTDFRITI
                     VEVANPPAKPDNALKMSPVYDINVSFANEFYLPISIKFKNIDKESFDHKNIDTYVAMY
                     FDDIAQEWVPFDKANISLQDTTLHLATYHFTKLAWFEMAHGSYTHIFTSKYVNVIYKY
                     GDGYPESNFYIRYNRQTDNQGRQYWHDTNNNPDADPYGTPYLIQDVAHYMDSIIHKFK
                     NMGLETPSLRFNVYVGLIGNNGMIDAGGYLAGRGYFYIDPQYVSYFDNQEENRHEMMR
                     TLAHEYMHYTQDYYMTVLLQNYYWMEATAPIAARIVWEENSLPDTEPELLIQDALYPE
                     RDNTNLVQRILMMGRNKTIFETLSDSWLSNYNIPVATKLRSNSADANIASLFLHYMQN
                     YREGKKLNATSLIKETSYLETWIEYLTSFVNKHLESTVGKEFDDYVRYVLSGQNTKFS
                     VLNSQGGNPFKHLIKNSESENTKAFALRQVYKFADTDKTPQKDEMSFTVPYLAAKIVL
                     LSNTSNKQLVVVNYKPSHEPNEHYKVYYGRYDSNLKKVEYTDISDSTSYNILLNTRTA
                     NAIDDARNTSFLLFVNTLAPSKTDVFTDFDASFELTASPVIAFTDVDYISVGTDFIHT
                     YSDSDVLMPFYISGAKRSFIAIDGEQILDYFRVDYTNSQISIVNDSTYKVSVQYQHSL
                     SISQNRPNLPASLQTKQLTQTIVYNFVLGKLTIEQHANITNKWGAYYDDFMDTHYPES
                     LGSTRTEHTKISLIGVNTFGMVDSDRLFFGTENTADTRKAILSMSDTSNATYYYSNGE
                     VSGTSSRSYVSTDFSSPSVKIYLWVHYDQ"
     gene            14637..>16484
                     /gene="thrS"
                     /locus_tag="PLJ82_03015"
     CDS             14637..>16484
                     /gene="thrS"
                     /locus_tag="PLJ82_03015"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOR40512.1"
                     /translation="MINITFPDGSVRQYEKGTTGLQIAESISSRLAQDVIAVGVNDQT
                     TEINRPITTDASIKLYKWDDAEGKHAFWHTSAHLMAEALQELYPHVKFGIGPAIENGF
                     YYDIDTADMVIRENDFPTIEAKITEIVARKENVVREEISKADAIKLFTEKDEPYKIEL
                     ISELEDGTISLYRQGNFVDLCKGPHLANTADIKAFKLLSVAGAYWRGDEKRKQLTRIY
                     AISFPKKKMLDEYLLLLEEAKKRDHRKIGKELELFTFSQSVGQGLPLWLPKGTMLRDQ
                     LEDFLKKVQKKYGYLPVRTPHIGQKELWITSGHYAKYGKDSFQPIHTPQEGEEYLLKP
                     MNCPHHCEIYKVTPRSYKDLPIRYAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQLKDEFMKVMDIIFIIFKALNFENFEAQISLRDPNNKEKYIGSDENWEKSEQAIIE
                     SCEAKGLKARVELGEAAFYGPKLDFMVKDAIGRRWQLGTIQVDYNLPERFELEYTGAD
                     NQKHRPVMIHRAPFGSMERFVAVLIEHTGGKFPLWLTPEQVVILPISEKFNDYAKEVV
                     NTLNMHDIRASVDERNEKIGKKIRDNELKRIPYMVIVGEKEAENNELSVR"
BASE COUNT         5611 a         2956 c         2872 g         5045 t
ORIGIN      
        1 caggtgcact ccataacaat tcatatttta atctaatagc agctgttttt acttgttgta
       61 atattctatc gtgcacattt tcctgctctg tatatccaat tttttgggag ccttcatttg
      121 gctttagttc aggaagagtg taggaatata tctgaggata tacaacttta taagacttta
      181 tgttttgtgt tatgttgctc attatgtcac ttttataact tatgtttcat tgttgttcgg
      241 gtgtctttta gcatgttgcc taacatctgt gtaggtacca cgttattgta taggtaccac
      301 agcgtggtaa ctatattcac tttatatacc tattcgtatt tgtttaaact gttgataatc
      361 tatagtttat gatttattaa tagaaatata ctatggcacc tatccatttg ttaatttatg
      421 tttaaatccc caattatcct tagctttttc agtttttaat tcaagaattc tatttttttt
      481 gtaaaactac taaagatttt ctgattgtaa aaactaaatg tgagttttta gataaaattt
      541 ttagtacaag catcaatgtt acaaacgagg tataaagaga ttaccctatc ctccacaagt
      601 ggactgtagt tattctttcc attttttgtc gggtattgat ttgtaacgtc cccaacatgt
      661 ataatgtgtc aatgatagta aggatacaat tttagatagt attctgtatt ttagtaacat
      721 atgctgtatg taatacaaaa tccacaaaat tgcaatgaaa ataaccgacg atagagtaaa
      781 tcttataaat gaagagtgta tctccaattg caaaacacgt tctactacta tagtaaatgc
      841 tacagaactt actatgctta ctcctataag taacccgtgt gctgtttcaa tggctctttt
      901 ggggcaatta ttcatacaac gcatacaact ttcacactta aaagtccaaa aaggatgttt
      961 atgaaatagg ttaattgcct gtacaggaca ttgtttaata cacaaaccac acgaatcaca
     1021 atctatcgta gcataaaatg attttgccaa tataaatctg cctatgaagt aataagctat
     1081 agcaatgggc gaaataagta tatcctgaat tacgtctctg tatgcaagaa aatcccgctg
     1141 acctgagaaa atgttgactg catgtttttc tactctattg taatttgttc tgtggaggta
     1201 ttttaccgtc ttttcattca atgctggatg tatcgaaagc caattagaag gcatatcaaa
     1261 aggaatttgc cctacaattt tatagccttt gtttcttaat atgaatgacg atacaaaaaa
     1321 tgcaattccc gttaagcctg gagttacaaa attccctatt ttcattcctg ctcgtgtatt
     1381 catcaaaaca acacgattgt ttcctttcgg aaagttacga ataaaatcca atgtaatttt
     1441 tggataatta aacccatgta tcggagaaat tatgcatacc aaagcatcat caattggagg
     1501 tatattttta tcacattttg caatgtcaac tatttcgcaa tctattctgt ttttgattgc
     1561 aaattcagaa aactacaaag caatgcgttt tgcattacct gttcctgaaa aataaaatag
     1621 gataactttc tgaatagtat tcatcgcctg ttgttaattt ctctatattc tatattctac
     1681 ctaacggcgg tctcaaaaaa cctccattaa gttgattttt tgttcaaaag taatgcgttt
     1741 tagggacgtt tcaaaattta ctggtatatt tttgatatta ttgagtagaa ttgtatctaa
     1801 taaataatat tttttgttac atttgcgttt caaaaaaaaa taacataaag tatgaacaaa
     1861 tctatctggc tattggcatt cgttgtttta ccaacttggc tatttgccca acaaacactt
     1921 aatgaaaaac ttattttaca actagttaac gaaattcgta ccgaaggatg ctattgtggc
     1981 aacgaagaag taaaacctgt aaacgaagta ctttggaacg ataaattagc aagcatagcc
     2041 acccaacata gccacgattt atttacatat acaagcaaaa atgagaataa atttgtttat
     2101 ctcagtcata taagtacgga tggctctact cttgacgacc gtcttaaaaa agcgaacgtt
     2161 accgcgaata acatacgcga aaacattgga tatgttcaag gaaacgaacg tatcattgtt
     2221 gattattgga taaacaaccc agaatcatgc aaaaatttaa tgcatccaac agctgccagc
     2281 ataggcattg gacgcagtgg aaatttttgg acaatgttat taacaagtgc ttttattaca
     2341 aaataatttt tttacctttg tgcgttttac aaaagaaata acttaaccta ataataaact
     2401 gttatgcaaa caattgacaa ttttaatttt gcaggaaaga aggcgtttgt acgcgttgac
     2461 ttcaatgttc cgttagacga aaactttaac attaccgacg atacccgtat tcgtgcggct
     2521 atgcctacgt tgaaaaaaat ccttgccgat ggtggtagcg ttattattgc atcgcacatg
     2581 ggcagaccaa agaaaaatcc agatgcaaag tattctctac aatacattct taaacacgta
     2641 gaaaaattac taggtgtacc tgtaaaattt gctgccgatt gtgcaaaagc tagcgatatg
     2701 gctgcttcgc taaaaaacgg agaagcatta ttactcgaaa atcttcgttt ttatgccgaa
     2761 gaagaaggca aagctcgtgg attagccgaa gatgctactg acgaagaaaa agctatagct
     2821 aaaaaagcaa ttaaagaaag tcaaaaagaa tttaccaaaa cattggcatc ttatgctgat
     2881 tgttatgtaa acgatgcttt tggtactgct caccgtgctc acgcttcaac tgcgttgatt
     2941 gcaaaacact ttgacgaaaa caacaaaatg tttggttatt tgatggaaaa agaagtattg
     3001 gctgttcaaa aaatactatc agatatcgaa cgtcctttca ccgctattat gggtggttct
     3061 aaagtatctt ctaaaattga aattatcgaa aacctgttgg gtaaagtaga taatttaatt
     3121 cttgctggtg gaatgactta tacctttagc aaagcaatgg gtggtaatat cggtatttct
     3181 atctgcgaat cagacaaatt agacttagct cttgagttaa tagaaaaagc aaagaaaaat
     3241 aatgtaaact tggtattggc tgtagatgct aaaattggtg acgatttttc taacgactgt
     3301 aacacacaat ttgtgtctgt taaagaaatt cctgatggat gggaaggtct tgacattggt
     3361 ccagaaactg aaaaaatatt tgcagaagta attaaaaact ctaaaacaat cttatggaat
     3421 ggtccaacag gtgtatttga gttcgaaaac tttacaagtg gctctaaagc tgtagcgaac
     3481 gctattgttg aggccactaa gaaaggtgcc ttctcacttg ttggtggtgg cgactctgtt
     3541 gcttgtatca ataaatttgg attggctgac caagtaagct atgtatctac aggtggtggt
     3601 gcgttacttg aagctatcga aggaaaagtt cttcctggta ttgaagctat tcgtggtttc
     3661 taacttaaga ttccctactt ttatcataaa aatgctatcc acacgggtag catttttttt
     3721 acaaattatt gtagacaggt atctcgttca aaaaaatgta ttgctgcaaa tcagtatcta
     3781 gtttacaaca aaaatgttgt ttcccattgc ttataagaat gtatggaact tgcaacacac
     3841 gattgtatag catcacttga tcaaataccg cttggcttat ctgtatgtca ggtgatttgt
     3901 attctattag cataattggt gtgtgtgctg tggaataaat tactgtatcg caccgcttat
     3961 tcattgtgtt tatacgtaat acacattcgt taaaaatcat tgttttagga taagctcgtt
     4021 cattttgtaa atacgcacac atctgttgac gcacaagctc ttcgggcgtg gcacgcacat
     4081 acttagcacg ataagcatcc aaaatatatg ttttttccgc ctgctttttt acacgaaaag
     4141 tgtaacgagg taattcaaat tccaacattt ttagtacttt tgctgtaatt aacaccgcaa
     4201 aggtatgaaa acaaaacaag aaatcataca aaattggcta ccacgatata ctgaacgtcc
     4261 attaaaagac tttacagatc ttattttgtt gacaaatttc aataattatg tagatatttt
     4321 tgctgaatgg tacaacgttc ctgtgttagc aaaaaatggg atgatgcgca atgcttcggc
     4381 tgaaggcatt accattatca attttggtat gggaagccca aatgcagctc tgataatgga
     4441 tattctacct gcaataaaac caaaagctgt tctatttctt ggaaaatgcg gtggattgcg
     4501 tgacaagaat cggcttggcg actatatttt acctagtgca gctatacgag gcgaaggaac
     4561 gtctaatgac tattttcctc ccgaaatacc tgcattacct gcatttagtt tacaacgggc
     4621 tgtttcgtca aatattagaa acatggaaaa agactattgg acaggaactg tgtatacgac
     4681 taatagacgc gtttgggaac acgacgaagc atttagagct tacttaatga aaacaagagc
     4741 catgggtatt gatatggaaa cggctaccct attttctgcg ggtttttaca atggaattcc
     4801 tattggagcc ttattgttgg tgtctgatat gcctttacaa aaagatggta taaaaacgag
     4861 cgatagcgac aaacatgtaa cagccaattt tgtagaagaa cacctgctta ttggaattaa
     4921 atctctctct tctatcaaaa ggcaaagtaa tactatcaaa catttacgtt acgaataaga
     4981 tacagaactg tggcaaaaaa gactcttaca tatacggaaa ttattacttc tttgcaggca
     5041 aaaaaatttg cccctattta cctgttaatg ggtgaagagc cttattacat tgacctcatt
     5101 tccgattaca tcgaaaaaaa tattcttacc gaagatgaaa aaatgatgaa ccaagtcgtt
     5161 cgttacggaa aagattctga tgtgggaagt attatcaatg aagctagacg ttttcccatg
     5221 atggctactc atcaagtcat catagtaaaa gaagctcaaa tgctagataa aattgagcaa
     5281 ctagaaatgt atcttcaaaa acctctggag actactattt tagttattaa tcacaaatat
     5341 aaatcggctg ataaacgcaa aaaatgggta acattagctg aacaatgcgg tgttgtattt
     5401 gaatcaaata agctacgaga ttaccaaatg cctgctatga tagaacaaat ggcacgagaa
     5461 aaaggcatgc aaataaacga aaaagccaag gctatgctta ccgattttgt aggaacagac
     5521 ttagctcaaa tggctggagt aatagataaa ttacgcatca tcttgcccaa agaaaacaac
     5581 caaattactc ctgaaataat tgaactgaat attggaatta gcaaagattt taatgctttt
     5641 gaattgacca atgcacttat acgtcgcgat gtacttacag ccaaccgcat cgttcattat
     5701 ttttcccaaa atgaaaaaga gcaccccata caagcgaccc ttgctgtgtt atttaatttt
     5761 ttctctaatt taatgtgttt tcattttttg ccaaaccaac aagatgatta tgtggcaaaa
     5821 gaattgaaaa tacactcctt ttttgtaaaa gactacaaat atgccgcaaa agcatacaac
     5881 aaaacacagg tgtttaagat tattagtcta ctacgcgaat acgatgccaa atcaaaagga
     5941 ataaaaaatt cttccgcttc tggaggcgaa ttactaaaag aattggttta caaaattatg
     6001 cattagtctt ggctaagggc ttcgaattcg gtatacagcc acattgccac atggttttct
     6061 gattgattct ttttcagatt ctgcatttca ttgataatgt tctccttaaa ctcatttttc
     6121 tggtaagcat acaataaaaa atacagcatt gattgagcca atttggctgt tatatggatg
     6181 gaagaaaatg ataaaagtgg agaaaattct tctgtcagtt ctggtttatt acgtttacaa
     6241 tagtgagcag ccaaggtata cgcagcaatt atttgttttt cttctgaaat acgggcaagt
     6301 tgtgttatca aagtgggaac aaaaggagta aataccacta aatgagcaca taagtactct
     6361 attaattcca ctgtatcgca atcagatatc cacgttatag atttttcgag agtgagtgct
     6421 tgtgcaggat acaaaagaat ggctaaaatt tttgtctccc ttaccgactt atgccataga
     6481 tacgtagcta ctaactcatt aggagaatat tgttgggcta tcttctgcaa atcgggaaaa
     6541 gcaacgccaa aattcgtttt atactttacg cccctatccg acattaattc ggaagtaacg
     6601 ccattcattg ctactcgaat ttttatcatt atctctttta cagtagcatc aacatcttgg
     6661 ttatatggta aaaatgtcat aattaagtta tttttaaatg acaaagatag tatttagctc
     6721 gtagcttaaa aaaaatacag ctaaaaaata cttaatacta ttgcataaaa tcaaacttag
     6781 tagtatcttt gcaacgtttt taacacacgg tgattgtagc tcagttggtt agagcgtcgg
     6841 attgtggttc cgaaggtcac gggttcgagc cccgcctttc acccttaaaa aagcaagtaa
     6901 caaatgacac acgattattg ttgcttgctt ttttgattta tgagctactt caggctatat
     6961 tattcttact tctacaacta ttttcataca tacttttaga aaatagaatt tacacagagg
     7021 gtatcgaatg tattttagtt aacaaacatt gaagattgtt aataaatatc tactttttat
     7081 tattaattat tcaatcaaaa tttgtagctt tgtgatacta tagttgtatt ggactattat
     7141 atagcttgca ccataacaag tataattgtt tttagatatg ttacaacgat acttagatac
     7201 atattgggtt aatttactat tgctgaaaag cgtagtgata agtgtattat tcatatcttc
     7261 ttgttctaca gaagaaccca cccccaagcc ttctactttg tgcaatatct ctgaaatttc
     7321 gtttgaatca gagcaatcag ctatgctcat tacagtaaat acgaaagaaa gttggtcgat
     7381 agaacatctg agcaattgga tagattttac tgcaacaaca ggacgtggca aaacttctct
     7441 tttagtaggt gcaaccaaaa atgatgggtt tggtcgcatc gactcattct taattaaaac
     7501 ggcaaaacaa acgcacaaaa tatacattga acaaaaagga gcagccattg tgcatcttga
     7561 tattttatcc catacactcg tttttgtgaa agttgaaggc ggaacattta agatagggaa
     7621 ttatcaaaat ccacacgaac atgatgtaag tctaactgag tattatattg gtcagacaga
     7681 ggtaacaaac gctttttggg aggctattgt aggctcacta ccatatagca acgaaaataa
     7741 aaattacccc tatacaatgg atgatgataa tccagatttt cctgttacag cagtttcatg
     7801 gaacgatatt cacactacat ttctacccct attacatact aaaacttcgc atacatttag
     7861 gctacctaca gaagctgaat gggaatttgc agcattggga ggaaataaaa gcaaaggata
     7921 tacattttcg ggtagtaata tacttgatgt agtaggttgg gatgcatcaa atgctaacga
     7981 tacaaaacaa gcagtagcac aattagaacc caatgaaata aacctatatg atatgagtgg
     8041 caatgccgct gagtggtgtt cggattggta tagcgattgg tacaatacaa cagaagatac
     8101 aacaaaccca actggacctg tttcaggcac atataaagta attcgtggtg gaagtattgc
     8161 ttctgaagcc ggaattcttg gttttcatcc ttgtcatgca aaacaacggt cttacgccca
     8221 tccatctggt tttcaaggag cttggggaga tacaggacat cctgatgaac cagttattta
     8281 taagtgcaac gagattggtt ttcgcttggt attggtacga taatattcaa caatagtgat
     8341 catgtttata agtaagcata tgtttcaaat caaattacat actctatttt gtatcatact
     8401 tatggtaagc atgagtagcc cttttgtatc cattgcccaa actgtaatca aaatggaact
     8461 tccaaaacaa acagaaaaag cgtttgcggt aaaaacactc tttaacgaac aattacccac
     8521 tggcgattta gttgttttag gtgccttagg ttatgaaata gagggaggtg caacacccta
     8581 tgaattagct tggtacgaga acaatcaact tatagcaaaa ggcaatatag tagcatttaa
     8641 gccacaagaa agtgcccaat attctcttgt tgtgaaagat aaaaataatt gttctaaaac
     8701 cttatccatc aatgtagatg cattaaaaag agctattaat gatacgcaaa agccaagcaa
     8761 ttttgtatct ctttcaccaa ctttggtaaa agaccatatt atgctaaggt gtcattctga
     8821 acctattgct tcagtacacc tcaaatttat tgatataaac ggaaatacat gtattgagac
     8881 gtcaattaca ggttctactt atttacctgt ttctttggca agtggcttac atatagtatt
     8941 aatacagtat ggagataatg tgtgggttga aaaaattatg gttgaataat gtattgcaaa
     9001 gaaaatgagt atgatacata aaaaaatata taccttattc gttatactat tgacactaac
     9061 agccaatagc tatggcaata gtgatgctat tcataaggta gctgatacgt ttttcaattt
     9121 cactaaagat ggtaaaagaa aagctgttgc agaaacaaag ccaatagaag tgaaatatgt
     9181 atcgataaat aacaacaaat cgaatgtttt tgttgtgcaa caagatggag aaggatttgc
     9241 catcattagt caacatggtg acgccagtag tgtggttgca tatattccta atggcgattt
     9301 tgaaaaagcc aaattatcgc cttcgtttat gtctgtgttg caaatctacg aaaacaataa
     9361 tccagaaacg acacctccct tacacacaaa atgggacgtt ttaggcacta cacaaccctt
     9421 gctcgatcaa gcaggtgttt ctcttaatca gttttatcat gcatcattag gaaactgtcc
     9481 gacaggttgt gttgctactg cattagcaca aataatggca tatcacaaat ttccaagcac
     9541 aggtaaaggt agtcattgct acacacaccc acaatatgga caacaatgtg ctgattttga
     9601 aaatactcac tataattgga ctaaccctac gtatgatgat tacgaactgt tatcttatca
     9661 tatcggtgta gctaccgaaa tgaattattg tggttcgaaa tatggcagtt ctccatcacg
     9721 ttccaactac tcagaagttc ttgaattata ttacggatat tcgatgagaa aaggaactcg
     9781 cgatatgaaa taccttaaaa ctgaaataga tgaaatgcgt cctgtatacc tcgaattagg
     9841 tggcgatcct ggacacgcag tagttataga tgggtacaaa gatgaaaatt atttacattt
     9901 aaattttggt tggggtggtc aagccaatgg gtattatatg atgaatacca atagtacttt
     9961 ttgggttggt tatacctttg gcaccaactt aggaagcaat attttttatt cccctactcc
    10021 ttttatcaca aacgagtctg attctttagc tcttattgcc atacacaacg cactcgatgg
    10081 tactacaggg tggaatacgt ctttacctgt atcgaagtgg cacggagtaa taacacatca
    10141 ttcaagggtg gtagaattag gattgggtgg atacgattat ggggtaaaag gaaatatcgc
    10201 tcctgaaatt gctctattaa acaaattaca acatttaaca atcattggta gcgaactcaa
    10261 tgggaatctt acagctgaca tttttcaact accagacctt gaatcccttc atatttcaaa
    10321 aaatgcgggc acattacaat gtgaatttcc tagtcaaatt gatggttgtc agaaactaac
    10381 tcacttatca atgccaggta tcctcactgg aaccatacct gaatccatcg gaaacctagt
    10441 tcaattacaa gaattatcta tttacaatgg aactctttct ggaagtattc ctttaacaat
    10501 aagtcaatta accaaactta aaacaataaa cataagtaaa aaccaattaa gtggacccat
    10561 tcctgatgct ttttacaact tggtagatgt agaaactctc gatttatctg aaaacaacct
    10621 ttcggggaat ctgcctattt ctctcgaaaa aatgactcaa ttgaaagaat tacggctaaa
    10681 caaaaacaac ctaagtggta tcctatcaga tgtgtttgaa catgtaaaga atattagtta
    10741 tttagatgta tccgaaaatc atttatctgg aaacgtgccc gagagtattg gtaagctaga
    10801 taaactacaa aaactgtttt tacataacaa ccaatttgtg gcattaccca acgatatgag
    10861 tggattagaa gcattgaatg aattactcgt caataataat ttactcacaa cattaccctt
    10921 gagtatcaat gctatttcaa gtttacgcac aatcgatgcc tctaacaacc aaatcactac
    10981 cttgcatcgt gattttggct tattaagaaa ccttcaatca ttgaatatat caaacaacca
    11041 acttacagaa atcccaagct caatattttt aaatgctaaa ctaggtacaa tcctattatc
    11101 caataataaa attaaagcat ttccgtcatc tatagatttt ttatcttcat ctatcgactt
    11161 tgcaaaattc gacgacaatg aattagaagg acaaatacct cttaatctac tcagtgcaaa
    11221 agcaacacat gtatcgctca taaacaatcg atttacgtat gaagatgtac ctgtatcaga
    11281 cgagctacgt catgcagttg ggttacaaaa aaaccatcgt ttaaaacaaa acaaattccc
    11341 ggtgatgatg ggtgatacca tccgtataga tatcagaaaa attagcaaat tatctcaccc
    11401 agacaatctt tattattggt tagaatatcc aaaatatgca gataaaaatg gaattgcctt
    11461 tttagaacaa ttgcagccaa atccaatttt aacggtggtt atcaacgaaa aaacagcagc
    11521 caaaaagttt tattgcaaag tgtttaacaa taacgtaccc acttggacat tcttttttaa
    11581 taattacgag tacatcaacc caacaatgag tgttgtaaat acagatacta ttgaatttga
    11641 aattctttcg gaagatgaga tgatggcata tatacatgct gaaagtaatt ttatacaatc
    11701 caaacagata ctaaacaaaa caatttcaga tcatatggtt actttggttc ctccattaac
    11761 cacttacaga ggttcgttgg tgtggcaagc ttctacagac ggaatcgcat ggcactctat
    11821 caccaacacg atggcaaatg aaaccattaa aggaaatatt gtttctatta aaaaggattc
    11881 tttggttatt tctccacttg ctgatgcttt ttaccgtact gccattgttg aatccgactg
    11941 taatcctttg tatagcgata cagtacacat aaaagcttat ggcaaattat tgtacgataa
    12001 agagataaac acaagcaatg atgatgtaaa aattgagtta gatgacattg aagtattagt
    12061 tcctaaaggc atacacaata ccgatttccg catcaccata gtcgaagttg ctaatcctcc
    12121 tgcaaaacct gataatgcac tcaagatgag tcccgtttac gatattaatg taagttttgc
    12181 taacgaattt tatctaccca tttcaattaa attcaaaaat attgataaag aatcctttga
    12241 tcacaagaac attgatacat acgtagcaat gtatttcgat gatattgccc aagagtgggt
    12301 tcctttcgac aaagccaata taagtttaca agatacgacc ttgcatcttg ccacctacca
    12361 ttttactaaa ttagcttggt tcgaaatggc tcacggatcc tatacacata tttttacttc
    12421 caagtatgta aacgtgatct ataaatacgg tgatggatat cctgaatcaa atttttacat
    12481 aagatataat aggcaaacag ataatcaagg tcgtcagtac tggcacgata cgaacaataa
    12541 ccctgatgca gacccatatg gtacgccata cttgatacaa gacgtagctc attatatgga
    12601 ttcaatcatt cataaattta aaaacatggg actcgaaaca cccagtttac gttttaatgt
    12661 gtatgttggc ttgattggta ataatggaat gatagatgct ggaggctatt tagctggtcg
    12721 tggatatttt tatattgacc cccaatatgt ttcatacttt gataaccaag aagaaaatag
    12781 acacgagatg atgcgtacat tggctcatga atacatgcat tatacacaag attattacat
    12841 gactgttttg ttacaaaatt attattggat ggaagcaact gctccgattg cagcacgtat
    12901 tgtatgggaa gaaaattcgc tacccgacac tgaacccgaa ctactcattc aagatgctct
    12961 ttatcctgaa cgagataata ccaatttggt acaacgaatt ttgatgatgg gtcgcaataa
    13021 aactatcttt gaaaccttga gtgattcttg gctttcaaat tacaacatac ctgtggcaac
    13081 caaacttcga tcaaactcag ctgatgccaa tatagcttcc ttgtttttac actacatgca
    13141 aaattaccgt gaaggaaaaa agctaaacgc tacatcgctc ataaaagaaa caagttattt
    13201 agaaacgtgg attgaatact taactagctt tgtaaacaaa catctagaaa gtactgttgg
    13261 aaaagaattt gatgattatg ttcgttatgt tttatctgga caaaacacca aattctcagt
    13321 actaaatagc caaggtggta atccttttaa acacctcatt aagaattcag aaagcgaaaa
    13381 caccaaagca tttgcactgc gtcaagtata taaatttgct gacactgata aaacgcctca
    13441 aaaagatgaa atgtctttta cggtacccta tttggctgct aaaatcgtat tattatcaaa
    13501 cactagcaac aaacaattag ttgtagtaaa ttacaaaccc tcgcacgaac ccaacgaaca
    13561 ttacaaagtg tattatgggc gctatgatag caatttaaag aaagtagaat atactgacat
    13621 ttctgattct acctcctaca atattttact caacacacga acggcaaatg ctattgatga
    13681 tgctcgaaac acaagctttt tactgtttgt caacacatta gctccatcta aaacagatgt
    13741 tttcactgat tttgatgctt cgtttgaatt aaccgcatct cccgttattg cctttacaga
    13801 tgttgactat atttctgtag gtactgattt tattcatacg tatagcgaca gcgatgtgct
    13861 catgccattt tacatctctg gagccaaacg cagtttcatt gcaatagatg gagaacaaat
    13921 cttggactat tttagggtgg actatacgaa ttctcaaata agcatcgtaa acgactccac
    13981 atacaaggtt tcagtacaat accaacactc attaagcatt agtcaaaata gaccaaatct
    14041 accagcctca ctccaaacta agcagctaac acaaacaata gtatataatt ttgtactagg
    14101 aaaattaacc attgaacagc atgccaacat caccaataaa tggggagcat attacgacga
    14161 ttttatggat acacactacc ccgaatcgct tggttcaaca cgtaccgaac acactaaaat
    14221 atccctaatt ggagtcaata catttggaat ggtagattct gaccgcttat tctttggtac
    14281 agaaaataca gctgacaccc gtaaagctat cctctcaatg tccgacacat caaacgctac
    14341 ctactattac agtaatggcg aagtaagtgg aacatcatca agaagctatg taagcacaga
    14401 tttcagttct ccttctgtaa aaatttacct gtgggtgcat tatgatcaat aaaagtgctt
    14461 attcattatt tcccgtttca actagtactg cttgaaacgg gttttttata cataacaatc
    14521 agattttaaa cgttacaaaa ccataaaata gcaaacgata aatagttttc aaaaaatatt
    14581 atttaccttt gcagtcatta caaaatttaa tcaatcacat tcgataaact atctatatga
    14641 ttaacattac atttcccgac ggttctgttc gtcaatacga aaaaggaact actggcttac
    14701 aaatagccga gagcatcagc tcacgcctag cacaagacgt gattgctgtt ggagtaaacg
    14761 accaaactac tgaaattaat cgcccaataa cgacagacgc ttctattaaa ttatacaaat
    14821 gggatgatgc tgaaggcaaa catgcttttt ggcacacctc tgctcactta atggctgaag
    14881 ccctacaaga attgtatcct cacgtaaaat ttggcatcgg accagctatc gaaaatggtt
    14941 tttattacga tatagataca gctgatatgg taattcgcga aaatgatttt ccaactattg
    15001 aagcaaaaat aactgagatt gttgcacgca aagaaaatgt agtgcgtgaa gaaatatcta
    15061 aagcagatgc tattaaatta tttactgaaa aagatgaacc ctacaagatt gaattaatca
    15121 gcgagcttga agatggcacc attagcctgt accgtcaagg gaattttgtt gatttatgca
    15181 aaggacctca cttggctaat actgctgata tcaaagcatt taaattgctt tccgtagctg
    15241 gagcatactg gcgtggtgac gaaaaacgca aacaattaac tcgtatttat gcgatttcgt
    15301 ttccaaagaa aaaaatgctt gacgaatatt tgttattact agaagaagca aaaaaacgcg
    15361 accacagaaa aattggtaaa gaattagaac ttttcacttt ctctcaatct gttggtcaag
    15421 gcttacctct ttggttacca aaaggaacga tgctacggga ccaattagaa gacttcttga
    15481 aaaaagtaca aaaaaaatac ggttatttgc ctgtacgtac acctcatatc ggacaaaagg
    15541 aattgtggat tacatctgga cattatgcaa aatatggtaa agattcattt caacctatcc
    15601 acacacctca agaaggcgaa gaatatttac ttaaaccaat gaactgtcct caccattgcg
    15661 aaatatataa ggtaactcca cgttcgtaca aagatttacc catacgatat gctgaatttg
    15721 gtactgttta tcgatacgaa cagagtggtg agttacatgg actaacacgc gttcgcagct
    15781 ttacacaaga cgatgcacat attttctgtc gtcccgacca gcttaaagac gaatttatga
    15841 aagtaatgga cattatcttc attattttca aagcattgaa tttcgaaaac tttgaagcac
    15901 aaatctcgtt gcgagaccca aataacaaag aaaaatacat cggtagtgac gaaaactggg
    15961 aaaaatctga acaagctatc atagaatcgt gcgaagcaaa agggctaaaa gcacgtgttg
    16021 aactaggaga agcagccttc tatggtccaa aactcgattt tatggtgaaa gatgccattg
    16081 gtcgtcgttg gcagttaggc accattcagg tggactataa cctacccgag cgttttgaat
    16141 tggaatatac aggggctgat aatcaaaaac accgtcctgt aatgattcac cgtgccccat
    16201 ttggctctat ggagcgtttt gtggctgttc ttattgaaca tacaggaggc aagtttccct
    16261 tatggctaac gcccgaacaa gttgttattt taccaattag cgaaaagttt aacgattatg
    16321 ccaaagaagt ggtgaatacg cttaatatgc atgatatacg tgcctcggtg gacgaacgca
    16381 acgaaaaaat tggtaaaaaa attcgtgaca acgaattaaa acgcatacca tatatggtaa
    16441 tcgttggcga gaaagaagct gaaaacaacg aactatctgt acgc
//
LOCUS       DAOSRP010000029        16274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3676_length_16274_cov_4.731549, whole genome shotgun sequence.
ACCESSION   DAOSRP010000029 DAOSRP010000000
VERSION     DAOSRP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16274
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3676_length_16274_cov_4.731549"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLJ82_03020"
     CDS             complement(<1..237)
                     /locus_tag="PLJ82_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HOR40513.1"
                     /translation="MRTLENFSLLNHNTFGVDVTANYFVEYDNITELQQLLGSDLLKE
                     NKFFHIGGGSNLLFINDYEGVILHSQIKGKEISKE"
     gene            388..1497
                     /locus_tag="PLJ82_03025"
     CDS             388..1497
                     /locus_tag="PLJ82_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40514.1"
                     /translation="MRKKQIILSVTNDLETDQRVHKVATFFYDKGFDVLVIGRKLKNS
                     QNLSRLYRTKRFNLFFNKSVLFYTEYTIRLFVFLLFSRKSVLWSNDTDTIIPNFIISK
                     LYKRVLFFDAHELFPELPELAHRPKIKMIWKKIEDCIFPHIQNSFTVCQSIADYYKEL
                     YGIEMQVIRNVPYKKDIRSYQPISVPKDKIIVLYQGAVNVGRGLEQSIEAMTLLPNAM
                     LYIVGDGDVLDQLKKQVQHLNVQQQVVFTGKIPFEELPRYTKVAHVGLALLEHRGLSY
                     YYALPNRIFDFIQADVPILARNFPEMARIINGYGVGITIDANTPSLIAKTIQDIYNQQ
                     HQYTLSSFDKAKEDLCWEKEQCILFEALKKSGLLN"
     gene            complement(1547..2494)
                     /gene="trxB"
                     /locus_tag="PLJ82_03030"
     CDS             complement(1547..2494)
                     /gene="trxB"
                     /locus_tag="PLJ82_03030"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOR40515.1"
                     /translation="MKTTETTRCLIIGSGPAGFTAAIYASRANLSPILYEGMQPGGQL
                     TTTTEVENFPGYPEGITGVEMMEDLKKQAIRFGTDVRWGSATEVDFSIRPFVVTIDGE
                     KTITADSVIIATGASAKYLGIPDEQKYAGSGVSACATCDGFFYRKKNVAVVGGGDTAC
                     EEAIYLSSICNKVYLIVRRNELRASKVMQKRVIETANIEVLFEHQTKSLFGEKVVEGA
                     ILWHKKGTPEEKEVSINIDGFFLAIGHTPNTRIFKNFIEMDEVGYIKTKNGSTKTNIE
                     GIFAAGDVADPVYRQAITSAGSGCKAAIDAEKYLHENGL"
     gene            complement(2502..3149)
                     /locus_tag="PLJ82_03035"
     CDS             complement(2502..3149)
                     /locus_tag="PLJ82_03035"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOR40516.1"
                     /translation="MKKTLFLLITLLSASLYLSAQVDAKAIEILDKATKDIKSATFTN
                     TDFTMHINNAQNKASQKMQGNIRTHKEKMMINTAFAEILYDGKFQYVYLKEANELTIS
                     EANAEELKQFNPTYVLNTYKKNYRIPQPAIVVENNQQVYSIDLYPENKNETYFRINIK
                     IRIDKNQIVNISTFEKSGITTSIELTKMNVNASLLASDFIFDAKKYPKAEIIDLR"
     gene            complement(3156..3719)
                     /locus_tag="PLJ82_03040"
     CDS             complement(3156..3719)
                     /locus_tag="PLJ82_03040"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOR40517.1"
                     /translation="MKQNWKPGTLIYPLPAVLVSCGSTEDEYNLLTISWTGTICSNPP
                     MCYISVRKERHSYPILVKNKAFVINLTTESMARATDWCGVRSGAKYDKWKEMNLTPEK
                     AQLVNAPIIKESPLNIECKVLEIKELGSHDMFIAEVVNVQADEQFMDAKTGEFKLSES
                     KLIAYSHGNYYKLGEKIGKFGWTVQKK"
     gene            complement(3721..4185)
                     /gene="pyrI"
                     /locus_tag="PLJ82_03045"
     CDS             complement(3721..4185)
                     /gene="pyrI"
                     /locus_tag="PLJ82_03045"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOR40518.1"
                     /translation="MKTNINHELVVQALKNGTVIDHIPSDKLFQVVSILALHTMPNQI
                     TIGNNLESKKLGIKGIIKISDVFFTPKEVNKIALIAPNAKINIIKNYEVIKKVELVIP
                     DEIKDTLQCINPMCITNKEHVPTHFYFIDKKKRLAKCHYCERICKDEDLMLK"
     gene            complement(4208..5116)
                     /gene="pyrB"
                     /locus_tag="PLJ82_03050"
     CDS             complement(4208..5116)
                     /gene="pyrB"
                     /locus_tag="PLJ82_03050"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOR40519.1"
                     /translation="MKNKSLVSISDFSKEDILRILVEAEKFENNPNTRILEGKVIAML
                     FFEPSTRTRLSFETAVNRLGGRVIGISDAATSSSTKGETLNDTIKMVSGYADLIIMRH
                     HIDGAARYASEVSAVPIINAGDGSNQHPTQTMLDLYSIKKTQATLENLQISLVGDLKY
                     GRAVHSLVMAMSHFNPSFHFVSPPELQIPDSYKLLCNKQGISYKQYVEISEAINKADI
                     LYMTRVQRERFTDPVEYEKVKNTYVLKNAMLENSKQNLRILHPLPRVNEIDMDVDTNP
                     KAYYFEQARNGVYVRQAIICSILGLL"
     gene            complement(5330..6574)
                     /locus_tag="PLJ82_03055"
     CDS             complement(5330..6574)
                     /locus_tag="PLJ82_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOR40520.1"
                     /translation="MAIIKPFKGIRPPQSIVEQVASRPYDVLNSEEARKEAEGNEKSL
                     YRIIKPEIDFPIGKDEHDADVYEKAAENFQLFQDKGWLVQDDSEKYYIYAQTMNERTQ
                     YGLVVCASVDDYMQEKIKKHELTRRDKEEDRMKHVRINNANIEPVFLAYPNNKEIDAI
                     VSKTIMQKATYDFVAPDGFGHHFWVINDTNDIHRITKLFANIPSLYIADGHHRSAAAA
                     LVGNEKKLQNPHHKGDEEYNYFLAVCFPDNQLNIMDYNRVVKDLNGLSDSDFLTALEK
                     NFVVEDKGTAIYHPNTLHNFSLYLSGKWYSLTAKPGTYNDNDPIGVLDVTISSNLILD
                     ELLGLKDLRSDKRIDFVGGIRGLEELKKRVDSGEMKVALALYPVSMKQLIDIADTGNI
                     MPPKTTWFEPKLRSGLVIHKLV"
     gene            complement(6664..7584)
                     /locus_tag="PLJ82_03060"
     CDS             complement(6664..7584)
                     /locus_tag="PLJ82_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR40521.1"
                     /translation="MKVLVATDKPFAKVAIDGIKKVVVAAGFELALLEKYTEKQQLLD
                     AVADTNAIIIRSDIVDAEVIAAAKQLKIVVRAGAGYDNVDLEAATAHNICVMNTPGQN
                     ANAVAELALGMMVYGVRNFYNGSSGTELLGKKLGIHAYGNVGRNVARIAKGFGMEIFA
                     YDAFCPAHVIEQDGVTAVQSAAELYKTCQLISLHIPATAETKNSINYELLSTMPKGGM
                     LINTARKEVINEAELIKLMEDRTDFKYLTDIPPSHHDEFTTHFPGRYFSTPKKMGAQT
                     AEANINAGIAAAEQIVNYILHGNDKFRVNK"
     gene            complement(7607..8674)
                     /gene="serC"
                     /locus_tag="PLJ82_03065"
     CDS             complement(7607..8674)
                     /gene="serC"
                     /locus_tag="PLJ82_03065"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOR40522.1"
                     /translation="MKKHNFNAGPSILPREVIEKTAQAVLDFENDGLSILEISHRAKN
                     FQPIVDEAVALFKELLNIPEGYSVLFLGGGASLQFCMLPFNLLEKKAAYLNTGAWAKK
                     ALKEAKAFGEVVEVASSADANYTFIPKGYSIPTDADYFHITTNNTIYGTELLEDITSP
                     VPLIADMSSDIFSRPVDVSKYGMIYGGAQKNLAPAGVTFVIVKDELLGKVSRYIPTML
                     DYRTHIKEGSMFNTPPVLPIYAALQTLKWLKSQGGLVQVEKNNIAKATLLYNAIDNSK
                     MFVGTANKEDRSRMNICFVMKPEFQHLEADFLKFATSKGMVGLKGHRSVGGFRASCYN
                     ALPIESVQALVDCMNEFETNN"
     gene            complement(8856..10073)
                     /gene="pepT"
                     /locus_tag="PLJ82_03070"
     CDS             complement(8856..10073)
                     /gene="pepT"
                     /locus_tag="PLJ82_03070"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOR40523.1"
                     /translation="MKSITERFLSYIEIDTQANETSNSTPSSMGQVLFAEMLEKELAS
                     LGLDVIRTDKSYVYATLKSNLTEKKIPTIAFIAHMDTSPAMSGKNIRPNIVKAYDGKD
                     IILQKEGILSPQQFPELLHYIGQDIIVTDGTTLLGSDDKAGIAAIVSAIEKICEDNNF
                     KHGDILFAFTVDEEIGKGADHFDLNLCRADWAYTIDGGEIGELEYENFNAAMATITIQ
                     GNSVHPGYAKNKMINSMLIAQEVISLLKQFGLPENTSGYEGFFHLDKIEGNVEKTILQ
                     YIIRDFDIQDFTNRKANLSTIVRQMNEKYPLACINVLIEDQYYNMKSTIEPIMHIVSL
                     AEQAMIEVGVTPKIKPIRGGTDGARLSYMGLPCPNIFAGGHNFHSKFEYLPISSLEKS
                     MQTILKIIQIVSR"
     gene            complement(10136..11194)
                     /gene="rfbB"
                     /locus_tag="PLJ82_03075"
     CDS             complement(10136..11194)
                     /gene="rfbB"
                     /locus_tag="PLJ82_03075"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOR40524.1"
                     /translation="MEKRTILITGGAGFIGSHVTRLFVNKYPNYTLYNLDALTYAGNL
                     ENLTDIANKPNYNFIKGDITDEIFIQELFQKYQFDGVLHLAAESHVDRSITDPLAFIK
                     TNVLGTANLLTAAKNLWKNNMEGKRFYHISTDEVYGSLGKEGFFTEETPYDPRSPYSA
                     SKASSDHFVRAYYHTYGLPVVISNCSNNYGANHFPEKLIPLAINNIKHSKPIPIYGKG
                     ENIRDWLWVNDHARAIDAIFHTAKIGETYNIGGHNEWTNIDLIKKLCEIMDTKLGRPQ
                     GTSAQLITYVSDRAGHDMRYAIDASKLERELGWTPSLQFDEGLEKTVDWYLANEEWLD
                     NVTSGNYQSYYEKQYTDR"
     gene            11351..12322
                     /locus_tag="PLJ82_03080"
     CDS             11351..12322
                     /locus_tag="PLJ82_03080"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40525.1"
                     /translation="MNTIICYIEELLYEHDFVVIPTFGGFVAGYQSSRITPEGNIYPP
                     QKEIGFNTNVQYNDGLLVQTMMEKQGISFSQASALLHVHIEEWKKELYREKNLHFGNI
                     GSFSLTNTNVISFTPAFTNDFLCSSFGFYPLYLPQLSQELVYNQAPVFVDTVRAFTPE
                     PISWSNTVRYIVASAAVILFFLFFPVQLTNIPIDSQAGVCGYRFTSAVSGNLSVDENE
                     RIEVVDNQQHKDSCIQKVNTLTTLNGVQQLPERFYIVIGSFQTERMAQRFVSELPAIF
                     SSATIVFSENRYRITTASFETEQEGEYFLHTFQQNHPRFKDAWLLKK"
     gene            12438..12968
                     /locus_tag="PLJ82_03085"
     CDS             12438..12968
                     /locus_tag="PLJ82_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOR40526.1"
                     /translation="MATKIRLQRHGRKGYAYYHIVVADSRAPRDGKFIERIGSYNPNT
                     NPATVNVNFEKALKWIETGAQPTDTVRNILSREGVMMKKHLLGGVKKGAFTAEEADKK
                     FDAWMKNKQVAVAKVKEKLASDASMTAKQRLDAEKAANKAKAAEVAKKQAEKLAVAEP
                     VAEEVATTETETSVAE"
     gene            13035..14591
                     /locus_tag="PLJ82_03090"
     CDS             13035..14591
                     /locus_tag="PLJ82_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOR40527.1"
                     /translation="MVLEKNCRVLPKIAATENLLKEYLAKELDITPKSIHAIHILKRS
                     IDARQRMVFVNLQIRIFIDEKPKEERVFLPTYKDVSQAKKQVIVVGAGPGGLFAALRL
                     IELGIKPIVMERGKDVSERKKDIALISTSHKVDADSNYCFGEGGAGAFSDGKLYTRSK
                     KRGNVDKILQVLCYHGASHSILIDAHPHIGTDKLPSVIKNMRETILSYGGEVYFETRM
                     DDLLIQHNQVKGVVTNTGQTFLGNVLLATGHSARDVYRLLQHKEVAIEAKGIAVGVRL
                     EHPQALIDQLQYHSKDGRGEYLPAAEYSYVTQVADRGVYSFCMCPGGFVVPSASAPNQ
                     VVVNGMSPSSRGSVWANAGMVVEIRPEDLPQYAEYKEFSVMRFQEDMENLAYVNGGSK
                     QTAPAQRMTDYVRKKVSYDLPASSYTPGIVSSPLHFWLPEQINERLREGFKTFGRWSN
                     GFLTPDAILLGVETRTSSPIRILRDRETCEHVSIKGLYPCGEGAGYAGGIVSAAIDGE
                     KCAESVSLVL"
     gene            14729..15169
                     /locus_tag="PLJ82_03095"
     CDS             14729..15169
                     /locus_tag="PLJ82_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR40528.1"
                     /translation="MTALELDQQVVFGILSGRASTAIARRLYREFRLNGLQVTPEQWV
                     ILMALTNKDGITQQELAITTYKDKPSITRLINNLEKQSLVVRSSSKSDKRMNQIYITK
                     SGLFLYEKAHMAAINVMKDALYGLIDDDIRMGERILKQIFKNLA"
     gene            15239..>16274
                     /locus_tag="PLJ82_03100"
     CDS             15239..>16274
                     /locus_tag="PLJ82_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR40529.1"
                     /translation="MKKLFVCLVLLVSIVLQVFAQKELFSLDDCRNMAMSNNKELKIA
                     HEQVKVAVSLEKAAFTHYFPTISANGAYLWNEKNISLMKEDAYLPVYAMDINGSKNYA
                     ASWNNSWAVYNGAPVPLDANGVPFDPQKNPEKISWKNYAYLPKDAFEMDVHNVFVGSV
                     MMAQPLFMGGKIRELNNIAKSTRKISEAKFAGELSDNMLETDAAYWRVVSLVNKEKLA
                     KSYVALLEKLSSDVEKSILFGVATKSEALSVKVKLNEAQMQLLQVQDGLNLSKMLLCQ
                     LCGLPLDSDFGLVDENIATTDMPLDNPTINIDNAVANRYEIKSLEETINIAESNRKIM
                     VSRFMPNAALT"
BASE COUNT         4997 a         2736 c         2865 g         5676 t
ORIGIN      
        1 ttccttgctt atctcttttc cctttatttg cgaatgtaaa ataacccctt catagtcgtt
       61 gataaagagc agattactgc cacctccaat atgaaaaaat ttgttctctt ttaacaaatc
      121 acttcctaat aattgttgca gttcagtaat gttatcatac tccacaaaat aatttgccgt
      181 aacatcgact ccaaatgtat tatgattaag tagtgaaaaa ttttccaatg ttctcatata
      241 atagtggata ttgtattagt attactaaaa ttgtataaat caattttcgg ttttttataa
      301 taattgtcca tctttgtttg ttgtacaaag atataaaatt tatacttcta agagctacaa
      361 atagtaaaaa aaacgtactt ttgtaacatg agaaaaaagc aaatcatact ttcagttacc
      421 aacgatttgg aaacagacca acgtgtacat aaggtagcta ctttttttta tgataagggt
      481 tttgatgttt tagtaattgg tcgtaaactt aaaaatagcc aaaatttatc ccgcttatat
      541 cgtacaaaaa gattcaatct atttttcaat aagagtgttt tattttatac tgaatatact
      601 attcgattat ttgttttttt acttttttct cgaaaatctg ttttatggtc gaatgatacc
      661 gatactatta tccccaattt tattatctct aagttgtaca aaagggtctt gttttttgat
      721 gctcacgaat tatttcccga gttgcctgag ttggcacatc gaccgaaaat taaaatgatt
      781 tggaagaaaa ttgaagattg tatttttcct catatacaaa atagttttac ggtttgccaa
      841 tccatagcag attattataa agaattgtat ggtattgaaa tgcaagtgat tagaaatgta
      901 ccttataaaa aagatatacg ttcttaccaa cccatatcgg taccaaagga taagataatt
      961 gttctttatc agggagcggt aaatgtaggt aggggtttag agcaatctat cgaggcaatg
     1021 actttattgc cgaatgctat gctgtatata gtaggagatg gcgatgtgtt agatcagtta
     1081 aaaaaacaag ttcaacattt gaatgtacaa caacaagttg tttttacagg caaaattcct
     1141 tttgaagagt tacctcgcta cactaaagta gcacatgttg ggttagcatt actggagcat
     1201 agaggtttga gttattatta tgcccttcca aatcgaatat ttgattttat tcaagctgat
     1261 gtacctattt tagcacgtaa ttttccagaa atggcacgaa ttattaatgg atatggtgtt
     1321 ggaataacca tagatgctaa cactccatca cttattgcta aaactataca agatatatat
     1381 aaccaacagc accaatacac tttgagttca tttgacaagg caaaagagga tttatgttgg
     1441 gaaaaagagc aatgtatatt attcgaagca cttaaaaaga gcggtttatt gaattaatct
     1501 acaacctcac catttttgat aaggttgtat gatacatatt tagctattat aatccgtttt
     1561 cgtgtagata tttttcagca tctatggctg ctttacaccc actaccagca gaagtgatag
     1621 cttgtctgta tactgggtcg gctacatccc cagcggcaaa tatcccttca atattggttt
     1681 tagtactacc gtttttagtt ttaatatagc ctacttcgtc catttctata aagtttttga
     1741 agattcttgt atttggtgtg tgacctatgg ctaaaaagaa tccatcaatg ttaatgctca
     1801 cttctttttc ttctggagtt ccttttttgt gccataatat agccccttct accactttct
     1861 caccaaaaag tgattttgtt tgatgttcga ataacacttc gatgtttgcc gtttcgataa
     1921 cgcgtttttg cattaccttt gatgctctta gttcatttct acgaacaatc aaatatactt
     1981 tattacagat agatgatagg tatatagctt cttcgcaggc agtatctcct ccaccaacta
     2041 ctgctacatt tttttttcta taaaagaatc catcgcatgt agcacatgca gatacaccag
     2101 aaccagcata tttttgttca tcagggatgc ccaaatattt tgctgatgcc cctgttgcga
     2161 taatgacaga atctgctgta attgtttttt ctccatcaat ggtaactaca aatggacgta
     2221 ttgaaaaatc aacttcggta gcagagcccc accgtacatc tgttccaaat cgtatggctt
     2281 gttttttaag gtcttccatc atttctactc cagtaattcc ttctggataa cctggaaaat
     2341 tttctacttc ggtggttgtt gtcaattgtc caccaggttg cattccctca tataaaatag
     2401 gggataaatt tgctcttgaa gcataaatgg cagcagtaaa tcctgctgga cctgacccaa
     2461 taattaggca acgagtcgtt tctgtcgttt tcatatctgt attatcttaa atctattatt
     2521 tctgcttttg ggtatttttt tgcatcgaaa ataaaatcgg atgctaaaag cgaagcattt
     2581 acattcattt tggttagttc gatactggta gttatgccag atttttcaaa tgtgcttata
     2641 ttcactattt gatttttatc aatacgtatt tttatattaa ttctaaagta tgtttcattt
     2701 ttattttcag gatacaaatc aattgaatat acttgttgat tattttccac cacaatggct
     2761 ggttgtggta tacgataatt ttttttgtat gtatttagca catatgttgg attgaattgc
     2821 tttagttctt ctgcattggc ttcactaatt gtgagttcgt ttgcctcttt taaataaaca
     2881 tactgaaatt taccatcgta cagaatttct gcaaatgctg tattaatcat cattttctct
     2941 ttatgtgtgc gtatatttcc ttgcattttt tgagatgctt tgttttgagc attattgata
     3001 tgcatggtaa aatctgtatt tgtaaatgtt gcagatttta tatcttttgt tgccttatct
     3061 agtatctcta ttgctttggc atctacttgt gctgataaat acagtgatgc acttaataat
     3121 gtaatgagta gaaaaagtgt ttttttcatt gtgtattatt ttttttgaac tgtccaacca
     3181 aattttccta ttttttcgcc taatttgtag taattaccat gtgaataagc aattagtttt
     3241 gattcggata gcttaaattc tcctgtttta gcgtccataa attgttcatc agcttgcaca
     3301 tttaccactt cagctataaa catatcatgg gatcctagct cttttatttc tagcacttta
     3361 cattctatat tcaaaggaga ttcttttatt ataggtgcgt ttactaattg ggctttctct
     3421 ggtgttagat tcatttcttt ccacttatca tattttgctc ctgatcttac accacaccag
     3481 tcagtagcac gagccatact ttcggtagtt aggttaataa caaaagcctt attttttacc
     3541 aatataggat acgaatgtcg ttcttttcgc acagaaatgt agcacatcgg aggattgcta
     3601 cagatggtgc ctgtccaact aatagttaaa aggttatatt catcctctgt actgccacaa
     3661 ctaactagta cagcaggtag tggatagata agtgtacctg gtttccaatt ttgtttcatc
     3721 ttatttcaac attaaatctt catctttaca aattcgttcg caataatgac attttgctaa
     3781 tctttttttc ttgtcaataa aataaaaatg tgtgggcaca tgctctttgt tcgttataca
     3841 catggggttg atacattgca aggtatcttt aatttcatca ggaataacaa gttctacttt
     3901 tttgataact tcgtaatttt taatgatatt aatttttgca tttggtgcaa ttagtgcaat
     3961 tttatttact tccttggggg taaaaaatac atcagatatt ttaatgatac ccttaatacc
     4021 aagtttttta ctttccaaat tatttccaat cgttatttgg ttaggcattg tatgtagggc
     4081 gagtatagat acaacttgaa ataatttatc agatggtata tgatcaataa ctgtcccatt
     4141 ttttagagcc tgtaccacca attcgtgatt gatatttgtt ttcatacgga taattttatt
     4201 gatttaccta aagaagtcct aatattgaac agataattgc ctgacgaaca tatactccgt
     4261 tgcgtgcttg ctcaaaataa tacgctttcg gatttgtatc cacatccata tcaatttcgt
     4321 ttacacgagg tagcgggtgt aaaatccgta agttttgttt actgttttct aacatagcat
     4381 tttttagtac atatgtgttt tttactttct cgtattctac agggtctgta aaacgttcac
     4441 gttgcactct cgtcatatat aaaatatcag ccttgtttat tgcttccgat atttccacat
     4501 actgtttgta agaaattcct tgtttattac atagcagttt atacgaatct ggtatttgta
     4561 actctggagg tgaaacaaag tgaaaggaag ggttaaaatg gctcatagcc attactaaag
     4621 aatgtacagc cctaccatat tttaggtcgc caaccaaact tatttgaagg ttttccaatg
     4681 tggcttgcgt ttttttgatg gagtacaaat caagcattgt ttgtgttgga tgctgattag
     4741 atccatctcc tgcattgata atcggtacgg cagatacttc tgaagcgtat cgtgcagcac
     4801 cgtctatatg atgtcgcata attattaaat ctgcgtagcc acttaccatt ttgatggtgt
     4861 cgtttagcgt ttcgcctttc gtagagcttg aagtggctgc atctgaaata ccaataacac
     4921 gccctccaag tctgtttacc gctgtttcaa aacttaatct tgtacgcgta gatggttcaa
     4981 aaaacaacat tgctattact ttaccttcca aaatgcgggt attcggattg ttctcaaatt
     5041 tttctgcttc tacaagaata cgcaatatgt cttctttgga gaagtctgaa atagacacga
     5101 ggcttttgtt tttcatcgaa accatttttt ttgtgtaata aataaactaa aatccaatta
     5161 caataaagcc actacaactt tcgttatact ggctttattg ttatggtaaa ttacatgttt
     5221 tcatacataa attttctaaa gtagatatac aaaaagattc atccatttct ttgctaatat
     5281 aatatcatac tacaaatgta taaaatatcg ttgatatttc atctgtttgt taaactaatt
     5341 tatgaattac caatccagaa cgtaattttg gttcaaacca agttgtttta ggtggcatga
     5401 tatttcctgt gtcagcaata tcaatcagtt gtttcataga aacgggatac aacgctaatg
     5461 ctactttcat ttcgccacta tcaacacgtt tctttaattc ttctaaacca cgtattcctc
     5521 ctacaaaatc aatacgttta tcgcttctaa ggtctttgag ccctagtagt tcatctagta
     5581 taaggttaga ggaaatagtt acatccaaca cacctatcgg atcattgtcg ttatacgtgc
     5641 caggtttggc tgtgagagag taccatttcc cacttaaata aagagagaaa ttatgtaatg
     5701 tattgggatg ataaattgct gtacctttat cttctactac aaagtttttt tctaatgctg
     5761 tcaaaaaatc actatcactt aatccattca agtcttttac tacacggttg taatccataa
     5821 tattcagttg attatcaggg aaacaaacgg ctaagaaata gttgtattcc tcgtctcctt
     5881 tatgatgggg attttggagt tttttttcgt tgcctactaa agcagctgct gcacttcgat
     5941 gatgaccatc agcaatgtat aaagacggta tgttagcaaa taatttggta atacgatgaa
     6001 tatcgtttgt gtcattgatt acccaaaaat ggtgtccgaa accatcagga gcaacaaaat
     6061 catatgttgc tttttgcata atagttttac taacaatcgc atctatttct ttattgtttg
     6121 ggtatgctaa aaaaacaggt tcaatattgg cattgttaat acgaacgtgt ttcatgcgat
     6181 cttcctcttt atcccttcga gtcaattcat gtttttttat tttctcttgc atatagtcat
     6241 caactgatgc acacacaacg agtccgtatt gtgttctttc attcattgtt tgagcatata
     6301 tgtaatattt ctccgaatca tcttgcacta gccatccttt atcttgaaaa agttgaaagt
     6361 tctcagctgc cttttcgtat acatctgcat cgtgttcatc ttttcctatt ggaaagtcaa
     6421 tttctggttt aataatgcga tacaatgatt tttcgtttcc ttctgcttct ttacgtgctt
     6481 cttcggaatt aagtacatca tacgggcgag aagccacttg ttctacaata gattggggtg
     6541 gacgtatgcc tttaaatggt ttaatgattg ccataatctt ttttactatt tgtttgataa
     6601 attaatctaa ttcaatgata tatataaaga aaaagcacct attcgtagat gcttttttta
     6661 tcgttattta tttacacgaa atttatcatt tccatgtaaa atataattga ctatttgttc
     6721 tgcagcagct ataccagcat tgatgtttgc ttcggctgtt tgagcaccca tttttttggg
     6781 agtagaaaaa tatctacctg gaaaatgggt cgtaaattca tcgtgatgcg acggcggaat
     6841 atcggtaaga tatttaaaat cagtcctgtc ttccattaat tttatcaatt ctgcctcgtt
     6901 gataacttct tttcttgctg tattgattag cataccacct ttaggcattg tagaaagtaa
     6961 ttcataattg atagagtttt ttgtctcagc agtagcaggt atgtgtaagg aaataagctg
     7021 acatgttttg tatagttctg ccgcagactg tacagcagtt accccgtctt gttcaataac
     7081 gtgtgcagga caaaatgcat cgtaagcaaa aatttccatg ccaaatcctt tggcaatacg
     7141 tgctacattt ctaccaacat ttccatatgc atgtatgcct aatttttttc ctaataattc
     7201 agtacccgaa gatccattgt aaaaattacg tactccgtat accatcattc caagagctaa
     7261 ttcggctacg gcattggcat tttgaccagg cgtattcatt acacaaatat tatgtgctgt
     7321 ggctgcttcc aaatcaacat tgtcgtatcc agcaccagct cttacaacta tttttagttg
     7381 tttggctgca gcaattactt ccgcatctac aatatcacta cgaatgataa ttgcgttagt
     7441 atctgctact gcatctaata attgttgttt ttctgtgtat ttttctaaca aggctagctc
     7501 aaatcctgct gctaccacta cttttttaat accgtcaatt gctactttgg caaacggttt
     7561 atctgtcgct actaatacct tcatgtttcg ataaaattat gtatgtttag ttattggttt
     7621 caaattcatt catgcagtca actaaagctt gtacactttc tattggcaat gcattgtaac
     7681 atgatgcacg gaaaccacct accgaacgat gaccttttaa tccaaccata ccctttgagg
     7741 tagcaaattt taagaagtct gcctctaaat gttggaactc tggtttcata acaaaacaaa
     7801 tattcatacg agatctatct tctttgtttg ctgtgcctac aaacatttta ctgttgtcaa
     7861 tagcattgta taacaaagta gcttttgcaa tgttattttt ttctacttgt actaaacctc
     7921 cttgcgattt taaccatttt aatgtttgca aagccgcata tataggtaat actggaggag
     7981 tattgaacat tgatccctct tttatatgtg ttctataatc tagcatggta gggatataac
     8041 ggcttacttt gccaagaagt tcgtctttta caataacgaa agtaacacca gctggggcta
     8101 gatttttttg tgcaccaccg tatatcatac catattttga tacatctact ggacgagaaa
     8161 aaatatcaga agacatgtct gctatgaggg gtactggaga agtaatatct tctaataatt
     8221 cagtaccata aattgtgttg tttgtagtaa tgtgaaagta atcagcatct gttggaatgc
     8281 tatatccttt aggaataaat gtataattag catcagcaga acttgcaact tctactactt
     8341 ctccaaaggc ttttgcttct tttagtgctt ttttagccca tgcacctgta ttcaaataag
     8401 ctgctttttt ctctaataaa ttgaatggaa gcatacaaaa ttgcaaacta gcaccgcctc
     8461 ccaaaaataa taccgaatag ccttcaggta tatttaataa ttctttgaac aatgcaacag
     8521 cttcgtctac aatgggttga aagttcttag ctctatggct aatttccaaa atagacaacc
     8581 cgtcattctc aaaatctaat acagcctgtg ctgtcttttc aatcacttct cttggtaaga
     8641 tagatggtcc tgcgttaaaa ttatgctttt tcattcgaaa aaataaatta aattagtttg
     8701 taaataatac ctacatgtag gttttttata attaattgga ttgtaaaaat ttgcgtttaa
     8761 aatcttcctg ttagctcaga ctttttagaa agcacaaaat tattgttttt tttcctatct
     8821 tgtatcaaaa agagaaggta tttttacaca agatattaac gacttacaat ttgaataatt
     8881 ttaagtatag tctgcatcga tttttctaaa gaagatatag gtaaatactc aaatttacta
     8941 tgaaaattgt gtccgccagc aaaaatgttg ggacagggta accccatgta cgaaagacga
     9001 gcaccatccg tcccgccacg tattggtttt atttttggtg ttacacctac ttctatcatt
     9061 gcttgttccg ctaagcttac tatatgcata ataggttcaa tagtggattt catattgtaa
     9121 tactgatctt caattaaaac gtttatgcaa gctaagggat atttttcatt catttgtcgt
     9181 actatagtgg ataaatttgc ttttctattt gtaaaatctt gtatatcaaa atcacgaata
     9241 atatactgta aaatagtttt ttccacattt ccttcaattt tatctaaatg gaaaaatccc
     9301 tcataaccac ttgtgttttc aggtaatcca aattgtttga gcaatgaaat aacttcttgt
     9361 gcaatgagca tagagttaat cattttattt ttcgcataac ccggatgtac gctgtttcct
     9421 tgaatggtaa ttgtagccat tgcagcatta aaattttcat attctaattc gccaatctcc
     9481 ccaccgtcaa ttgtgtatgc ccaatctgct cgacataaat ttaagtcaaa atgatctgcc
     9541 ccttttccaa tttcttcatc aacagtaaat gcgaataaaa tatctccatg tttgaagtta
     9601 ttgtcttcgc aaattttttc tatagcacta acaatagctg caatacctgc tttatcatcg
     9661 cttcctagca aggtggtgcc atcggttact attatgtctt gtccaatata gtgcaggagc
     9721 tcaggaaatt gttgagggga taagattcct tctttttgta gtatgatatc ttttccatca
     9781 tacgctttta caatgttggg tcgtatgttt ttcccactca ttgctggact tgtatccata
     9841 tgtgcaataa aagctatagt aggaattttt ttctcggtca agttactttt gagggtagca
     9901 tacacatatg atttatctgt tcgtattacg tctaatccca atgatgctaa ttctttttca
     9961 agcatttcag caaatagtac ttgacccatg gaactagggg ttgagtttga tgtttcatta
    10021 gcttgtgtat caatctcaat ataagataaa aaacgttcag tgatgctttt cattttgtgg
    10081 tatactaaaa agaggagtgt gcacacactc ctctttgatg attgaaattt gtatgttatc
    10141 tatctgtata ttgtttttcg taatagcttt ggtaatttcc actcgttaca ttatcaagcc
    10201 attcttcgtt tgctaagtac caatctactg ttttttctaa tccttcatca aactgcaaag
    10261 aaggagtcca tcctaattca cgttctaatt tgcttgcatc aatagcataa cgcatatcat
    10321 gaccagctct atctgaaacg tacgtaatta gttgggctga tgttccttgt ggacgaccaa
    10381 gttttgtatc catgatttcg cacaactttt taatcaaatc aatgtttgtc cactcattgt
    10441 gtccaccaat gttatatgtt tcaccaattt tagcagtatg aaaaatagca tctatcgcac
    10501 gtgcatgatc gtttacccac aaccaatcac ggatattttc tccttttccg taaatgggaa
    10561 ttggtttgct atgttttata ttattgatag ctagtggaat taatttttct gggaagtgat
    10621 tcgctccata gttatttgag caattggaaa ttactacagg taacccatag gtgtgataat
    10681 aggcacgaac aaagtggtca gaactagctt tagaagcaga ataggggctg cgagggtcgt
    10741 aaggagtttc ttctgtgaaa aaaccttctt tacctagtga gccatacact tcatcggttg
    10801 aaatatggta aaaacgtttc ccctccatgt tgtttttcca caaattttta gcagctgtta
    10861 atagattagc agtacccaat acattggttt taataaaagc taatgggtcg gtaattgaac
    10921 ggtcaacgtg agattcagca gcaaggtgca atacaccatc aaattggtat ttttggaata
    10981 attcttggat aaatatttcg tcggtaatat ccccttttat aaaattgtaa tttggtttat
    11041 tggctatgtc agttaagttt tccaaattac cagcatacgt aagagcatct aaattataca
    11101 gtgtgtaatt tggatacttg tttacaaata agcgtgttac gtgagaccca ataaaaccag
    11161 cacctcctgt aatcaaaatt gttctttttt ccatttaaaa aaatgtatta ggatttaacg
    11221 ggtacaaaat tacataaaaa gacgtatatt acaacaaatt gtacagaata ttttattgtt
    11281 tataagttgt tcaaaacttt taatttatct atattgcata gtagcattat tattacgtac
    11341 tttgtcggct atgaatacga ttatatgtta tatagaagaa ttattgtacg aacacgattt
    11401 tgtggttata cccacatttg gaggttttgt agctggttat cagtcttcta ggataactcc
    11461 agagggaaat atttatcctc ctcaaaaaga aataggtttt aatacaaatg tacaatataa
    11521 tgatgggttg ctggtgcaaa caatgatgga aaagcaaggt atcagttttt ctcaagctag
    11581 tgctttactt catgtacata ttgaagaatg gaaaaaagag ttgtatagag aaaaaaactt
    11641 gcattttgga aacataggca gtttttcact aacaaacacg aacgttatct cttttacccc
    11701 agcctttacc aatgattttt tgtgttcttc ttttggtttt taccctttat atttaccaca
    11761 gctatctcag gaattagttt ataatcaagc accagttttt gttgatactg ttcgtgcatt
    11821 tactccagaa cccatttctt ggtcgaatac ggttagatat atagtagcaa gtgccgctgt
    11881 aattttattt tttctgtttt ttcccgttca attaaccaat attcctattg attctcaggc
    11941 tggtgtatgt ggataccggt ttacctcagc agtgtctgga aatttgtctg tggatgaaaa
    12001 tgagagaatt gaagttgttg ataatcaaca acataaagac agttgtattc aaaaagtgaa
    12061 tacgctgact actttgaacg gtgtacaaca actaccagaa cgtttttata tcgttatagg
    12121 gagttttcaa acagaacgaa tggctcaacg atttgtgtca gaattgcctg ccatattcag
    12181 ttctgctact attgtgtttt ctgaaaaccg atatcgtata accacagcaa gttttgaaac
    12241 agagcaagag ggagaatatt ttcttcacac ttttcagcaa aatcatccgc gatttaaaga
    12301 tgcttggttg cttaaaaaat aaattttccc taaaaaactg ccgataagta tttgtcagct
    12361 taataattag tagtatcttt gcatccgcat tcgggaaatg cacagaagtt tattaactaa
    12421 taaaaaaaca aaaaagtatg gcaactaaaa ttagattgca aagacatgga cgcaaagggt
    12481 atgcttatta ccacattgta gtcgcagaca gcagggcacc acgagatggt aagtttattg
    12541 aaaggatagg ttcgtataac ccgaacacaa atcctgctac agtaaatgta aacttcgaaa
    12601 aagccttaaa atggattgaa actggtgctc aaccaacaga cactgtaaga aatattttat
    12661 ccagagaagg tgtaatgatg aaaaaacact tattgggtgg tgtaaaaaaa ggtgcattta
    12721 cagcagaaga agcagacaag aagtttgatg cttggatgaa aaataaacaa gtagctgtag
    12781 caaaagtgaa agaaaaatta gctagtgatg cttcaatgac agcaaaacaa cgtttggatg
    12841 cagaaaaggc agcaaataaa gcaaaagcag ctgaagtagc aaaaaaacaa gcagaaaaat
    12901 tggctgtagc tgagcctgta gccgaagaag tagcaactac agaaacagaa acctctgttg
    12961 cagagtaatt aaagtaataa tatgaaaaag agctgcccgt attgtatggc agctcttttt
    13021 ttatctaaat aagtatggta ctagaaaaaa actgtagggt attacccaaa atagctgcta
    13081 ctgagaattt attaaaagaa tacctagcaa aagaactaga tattacgcca aagagtatac
    13141 atgcaataca tatcctaaaa cgttctattg atgctcgtca gcggatggtt tttgttaatt
    13201 tgcagatacg tatctttata gatgaaaaac ccaaagaaga gcgtgtattt ttgcctactt
    13261 ataaagatgt atcccaagca aaaaagcaag tcattgtagt tggagctgga cctggtgggt
    13321 tgtttgccgc tttgcgatta attgaattag gaattaaacc gattgtgatg gaaagaggca
    13381 aagatgtatc tgaacgcaag aaggatatag cattaatttc tacatcccac aaagtagatg
    13441 ctgactctaa ttactgtttt ggagaaggtg gagcaggagc tttttcagac ggaaaactgt
    13501 atactaggag taaaaaaagg gggaatgtag ataaaatatt gcaggtgtta tgttatcatg
    13561 gagcatcaca ctctattttg attgatgccc atccccatat tggtacagat aaattgccta
    13621 gtgttataaa aaatatgaga gaaacaattc tttcgtacgg aggagaagtt tattttgaaa
    13681 cgcgtatgga tgatttgctt atacaacaca atcaagtaaa aggagttgta acgaatacag
    13741 gtcaaacatt tttaggaaat gtactattag cgacagggca ttctgctcga gatgtgtatc
    13801 ggttgctaca acacaaagaa gttgctatag aagccaaagg aattgctgtt ggtgttcgtt
    13861 tagagcatcc acaagcattg atagaccaat tacaatatca tagcaaagat ggtagaggcg
    13921 agtacctgcc agcagcagaa tacagttatg ttactcaagt agcagatagg ggagtatatt
    13981 cgttttgtat gtgtcctggt ggttttgttg tgccctctgc tagtgctcct aatcaggttg
    14041 tagttaatgg catgtctcct tcttcaagag gatctgtttg ggcaaatgct ggtatggttg
    14101 tagaaataag acccgaagat ttgcctcagt atgctgaata taaagagttt agtgtaatga
    14161 ggtttcaaga agatatggag aacttagcct atgtaaacgg aggaagtaag cagactgcac
    14221 ctgctcaacg gatgactgat tatgtgcgaa aaaaagtatc gtacgatttg ccagcctctt
    14281 cgtatacacc aggtattgta tcttctccgt tgcatttttg gcttcccgaa caaattaacg
    14341 aacgattacg agagggattt aaaactttcg gtcgttggtc gaatggtttc ctaacacctg
    14401 atgccatttt gttaggtgtg gaaacaagaa cgtcatcgcc tatacgaata ttaagagata
    14461 gagaaacgtg tgagcatgtt tccatcaaag gactttatcc atgtggtgaa ggtgctgggt
    14521 atgcaggagg aattgtgtct gctgctattg atggagaaaa atgtgctgaa tctgtttctt
    14581 tagtattgta gaaaataatg atattcttag aaaaacaaca aatgttgtat catgttaaaa
    14641 tatcgttaaa tatttttttt actaattaca tttcatctaa aacatataat tttgcaaggt
    14701 tgtttatgca actaataatt gtttaggtat gactgcattg gaacttgatc aacaagtagt
    14761 gtttggtata ttaagtggaa gagcttctac tgctattgct cgtagattgt accgagaatt
    14821 tcgcttgaat ggtttacagg ttacccctga acaatgggtt attcttatgg ctcttactaa
    14881 taaagatggt attactcaac aagaactggc tatcactaca tataaggata agccaagtat
    14941 tactagactt ataaataacc ttgagaaaca gtctttggtt gtacgctcct cttccaagtc
    15001 agataagcga atgaatcaaa tctatattac aaaatctggg ttgtttttgt acgaaaaagc
    15061 ccatatggca gccattaatg taatgaaaga cgctttatat ggattaattg acgatgatat
    15121 tcgaatggga gaacgtatct taaaacaaat atttaagaat ctagcataat tttttttatt
    15181 tattagttgc atatgcaact aaatatgtgt ttaattacta atttatagtt atattaaaat
    15241 gaaaaagcta tttgtttgtt tagttttatt agtatctatt gttttgcaag tatttgcaca
    15301 gaaagagctt tttagcttgg atgattgtcg taatatggca atgagcaata ataaagagtt
    15361 aaagattgca catgaacagg taaaagtagc cgttagtttg gaaaaggcgg catttacaca
    15421 ctactttcca actatatcgg ctaatggagc ttatttgtgg aatgaaaaaa atatttcatt
    15481 aatgaaagaa gatgcctatt tgccggtgta tgcaatggat ataaatggat cgaaaaacta
    15541 tgctgcttct tggaataatt catgggctgt atacaatggt gctcccgttc cgttagatgc
    15601 caatggagtg ccatttgatc ctcaaaaaaa tcctgaaaag atatcatgga aaaattatgc
    15661 ttatttacca aaagatgctt ttgaaatgga tgttcataat gtatttgttg gttctgtgat
    15721 gatggctcaa ccgcttttta tggggggaaa gattagagaa ctaaataata tagccaaatc
    15781 aacacgaaaa atttcagaag ccaaatttgc aggagagtta tcggataata tgttggaaac
    15841 ggatgcagct tattggcgag tggtttcttt agtaaataaa gaaaaattgg caaagagtta
    15901 tgtggctttg ctagaaaaat taagcagtga tgtagaaaaa tcaattttgt ttggtgtggc
    15961 aacaaaatcg gaagcactgt cggtcaaagt taaacttaat gaggctcaga tgcagttgct
    16021 ccaggtgcaa gatgggttaa atctttcaaa aatgctacta tgtcaattat gtgggttgcc
    16081 attggatagt gattttggtt tagttgatga aaatattgct accactgata tgccattgga
    16141 caatccaacg ataaatatag ataatgctgt ggcgaataga tatgagataa aaagtttgga
    16201 ggaaactatt aatattgctg aatctaatcg aaaaataatg gtttcgaggt ttatgcctaa
    16261 cgcagcctta acag
//
LOCUS       DAOSRP010000030        15760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3837_length_15760_cov_5.378924, whole genome shotgun sequence.
ACCESSION   DAOSRP010000030 DAOSRP010000000
VERSION     DAOSRP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15760
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_15760_cov_5.378924"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            669..1016
                     /locus_tag="PLJ82_03105"
     CDS             669..1016
                     /locus_tag="PLJ82_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40530.1"
                     /translation="MKKITLLLTFLLFTFVAYSQNDTIFLKSGEIYTVYRVLDIEDGY
                     LTFKRNKDSNISTIDASLVKTIGFQPKQLRDATDAYDKAYIIEGSFYEKNKKLSESAY
                     NDVGTNSRKTRHT"
     gene            1013..1723
                     /locus_tag="PLJ82_03110"
     CDS             1013..1723
                     /locus_tag="PLJ82_03110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR40531.1"
                     /translation="MINNDKVCLITGASSGIGFAIAKALHADNFEVIATARRLNKLTE
                     LGLDSNDLLAGDLNLPEFQDSIVDYIFDRYGKCDFLFNCAGTLEVGTIEDIDIDKLTS
                     MIRLNVETTFRLTYNILKRFKKQGFGHVINISSVLGTKVRPTAGGYAATKFAMEALSE
                     ALRMELTGTNIKISCIEPGLVMTELHNDWKVHPKESMNIHDPLTVDNIVDTVRFIINQ
                     PDHVRIPKLMILPKDHNI"
     gene            1933..2103
                     /locus_tag="PLJ82_03115"
     CDS             1933..2103
                     /locus_tag="PLJ82_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40532.1"
                     /translation="MATSILYYTGSFDKELLYVSAGLSIAALAIDFHSYKWIKRASIK
                     PSLQSLSVQLEF"
     gene            2271..2720
                     /locus_tag="PLJ82_03120"
     CDS             2271..2720
                     /locus_tag="PLJ82_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOR40533.1"
                     /translation="MKLQAICPISSQQIDERIPRINGAITVLLLVLFALTQHYFIILF
                     LVADFFARAIEKPDYSILAKLSKAVLARFSTTGKKTNAGPKIFAARIGLVFSLSIAIF
                     SLFGVDSVATVLVVIFGICAFLEAAFNFCVACKIYPFVYKFTHLNEM"
     gene            complement(3425..3823)
                     /gene="ssrA"
                     /locus_tag="PLJ82_03125"
     tmRNA           complement(3425..3823)
                     /gene="ssrA"
                     /locus_tag="PLJ82_03125"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3835..5364)
                     /gene="rny"
                     /locus_tag="PLJ82_03130"
     CDS             complement(3835..5364)
                     /gene="rny"
                     /locus_tag="PLJ82_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOR40534.1"
                     /translation="MTIIYIILGFVGGATVSAIVVQIVQKARAKSIIDAAQIEAETLK
                     KSKLLEVKEKYIQLKAEYEKQMTTRNAKIQSIEAKIKQKELFIENEEKQLEKKAAELE
                     SIKLNYENQLSLNEKKEEELDRLHHQAVQKLEAVAGLSAEEARNNLIESLKNEAETTA
                     ASYINEIMDEAKMTANKEAKKIVVNTIQRVATETAIENSVTVFHIESDELKGRIIGRE
                     GRNIRTLEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEV
                     VAKVTKQIEEEIVEIGKRTTIDMGVHGLHPELVRLIGKMKYRSSYGQNLLQHSRETAN
                     LCAVMAAELGLNPKKARRAGLLHDIGKVADEEPELPHALLGMKLAEKYKEKPDICNAI
                     GAHHDEVEMQTLLAPIVQACDAISGARPGARREIVEAYIKRLNDLENIALSYPGVMKT
                     YAIQAGRELRVIVGADKIDDQATERLSQEIAKRIQDEMTYPGQVKITVIREIRSVSVA
                     K"
     gene            complement(5474..5806)
                     /locus_tag="PLJ82_03135"
     CDS             complement(5474..5806)
                     /locus_tag="PLJ82_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOR40535.1"
                     /translation="MIDMEKQDLLTIRVMILDTSYPIKIRREEEEIYRKAAKQLNERI
                     LAYRKKFDRNLNDIDYVIMVAFELSLLSLRLEEERDNSPFISTIQALDNRLAEYLKNN
                     QLDTKNEE"
     gene            complement(5799..6092)
                     /locus_tag="PLJ82_03140"
     CDS             complement(5799..6092)
                     /locus_tag="PLJ82_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40536.1"
                     /translation="MTEEHDKLLKSFESRISMLLQKMDSYQSQLATLKDNYDRKNKEL
                     MHAHKTIVELQEKYDNLKIAKTVALSKTDIKDGKLRLSKMMREIDNCIALLND"
     gene            complement(6241..6588)
                     /gene="rplS"
                     /locus_tag="PLJ82_03145"
     CDS             complement(6241..6588)
                     /gene="rplS"
                     /locus_tag="PLJ82_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR40537.1"
                     /translation="MDLLKVAEASFASEKELPAFKSGDTITVSYKIIEGNKERVQQYR
                     GVVIKIVGHGVKKRFTVRKVSENVGVERIFPMDSPFINAIEVNKNGKVRRAKLYYLRA
                     LTGKKARIKERKR"
     gene            6809..7558
                     /locus_tag="PLJ82_03150"
     CDS             6809..7558
                     /locus_tag="PLJ82_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOR40538.1"
                     /translation="MSKLYILLVFVLGVGLATYFVRQPSQQITIHSEAILVNADVGLS
                     PCVAIDEFVQPFKDTIDSEMNTVIGYAPQDMTVGRPQSLLSNFVADLLLERANTIIPT
                     AISVVNMGGLRNSIKKGDVTVRSVFAVMPFENECVIVEMSGEAIWQLANEIVAVKGEG
                     FGGMQVVVKNNTLQQAYVQGKLLDRKASYRVVTSDYLLTGKDGLTALLQHKVLVNPAI
                     KMRDVILEHIVNETKQGKPIIAEMDNRYQIE"
     gene            7563..8390
                     /locus_tag="PLJ82_03155"
     CDS             7563..8390
                     /locus_tag="PLJ82_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOR40539.1"
                     /translation="MKQRTYIFPLFILISILFSCTSGERKLVILHTNDVHSAIEPNEK
                     GEGGVAMRCAIIDSIRQSEGDILLVDAGDYFQGTPYFNFFGGAVEIEAMNNMGYDVIT
                     LGNHEFDNGVEALVKQLTSSKATIVSTNYDVSETALKGIVFPYTITEKNGIKVGIFGL
                     GVSPEGLIRNDLFGKIHYKDPINSANKMAFNLKKNEKCDVVVCLSHLGYRYTNNTEKV
                     CDSILAIRSADIDVIIGGHTHDLIVNKKIKNKMGKEVTVAQMGKNGRYVGEIKISIN"
     gene            complement(8432..9493)
                     /locus_tag="PLJ82_03160"
     CDS             complement(8432..9493)
                     /locus_tag="PLJ82_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOR40540.1"
                     /translation="MRKNLFIVFIISIIIPISTFARVKNVIFLIGDGMGLNQIHASSI
                     VADSALNMQRAHYIGLVDTHSADHYITDSAAGGTALACGVKTKNGMIGMNPDSVAIPS
                     ILRIAHTNKLSTGVVVTSSVTHATPASFLAHQVNRKMEEEIAVDILHSGVDVFIGGGR
                     KFFSEREDKQNLVDSLHAKGYGVYSSIKEASISDSNKKGVLLADVGMTSKFEGRGNML
                     SEGTQLALDVLSKNKKGFFLLIEGSQIDWAGHANDIDGIIEETLDFDKAVGVAMDFAD
                     KNKETLVIVTADHETGGLQILGKDTDTKKLQTIFTSSYHSPVPVPLFAYGKDADKFSS
                     YMENTTIKKILVKLYRFKK"
     gene            complement(9509..9871)
                     /locus_tag="PLJ82_03165"
     CDS             complement(9509..9871)
                     /locus_tag="PLJ82_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOR40541.1"
                     /translation="MEEATNNIQESKQKLYFSIKEVAELVDVNISAIRFWEQQFDKIK
                     PRKNDKGTSFYTREHIDEFLLIKHLKEEKGLTLDGIKKRLKENKASTKKNFEIIDTLQ
                     KIKKQLHNIKTELDDLIE"
     gene            complement(9880..10854)
                     /locus_tag="PLJ82_03170"
     CDS             complement(9880..10854)
                     /locus_tag="PLJ82_03170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOR40542.1"
                     /translation="MGKAKYHFNEHTLNYDRIDNTIRHRLIKIGQYLIGSILMGIVFF
                     VIFIFTVKSPNEKLLEAENKRISTQYELLSRQLDDAMEVLTNIQQRDDNFYRVVLQVD
                     SIPSSIRRRNYINSSRYQKLLRMRTSDLVIATSQKMDMVSRMLYIQSNSFDELVALAK
                     QEEKKLQHIPAIQPIPNKDLKRTASGYGWRIDPIYHTRRFHKGMDFSAPTGTKIFATG
                     NGTVIKAGWEQGYGNAVFIDHGFGYVTVYAHMHQLKKGLRRGSKVSRGEVIGFVGNTG
                     KSTGPHLHYEVRLKGKPQNPQNYYFMDLSPEEYDQMIQISANYGRVFD"
     gene            11086..13707
                     /gene="alaS"
                     /locus_tag="PLJ82_03175"
     CDS             11086..13707
                     /gene="alaS"
                     /locus_tag="PLJ82_03175"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR40543.1"
                     /translation="MKSAKEIRESFTSFFASKGHKIVPSAPMVIKNDPTLMFTNAGMN
                     QFKDIILGNATVVYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKDAIDWAWEYLVEVLKINPEILYVTVFEGNPAEGLERDNEAAAYWAQYLPQDRIING
                     SQKDNFWEMGDTGPCGPCSEIHVDLRSAEEKLKVPGVKLVNESHPQVIEIWNLVFMQF
                     NRKADGSLELLPAKHVDTGMGFERLCMAIQGKTSNYDTDVFQPIIKAMATLAGVSYGE
                     NNEQDIALRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRAVRYGYTFLNQRSSF
                     MHALLPALIDSMGSAYPELEAQKGLIEKVIKEEEESFLRTLETGIRLLDKVITEAKLA
                     GKTTISGKDAFVLYDTYGFPLDLTELIAKENNVMVDVAEFEQEMQKQRERARNAASVE
                     TGDWIVVRDGESVFDGYDFAEMDVQLLRYRKMVQKGKYLYQLVFDRTPFYGEMGGQVG
                     DTGYILADTKKIAIVDTKKENGLTVHITLELPETIDATYTAVVNVEKRKRIAANHTAT
                     HLLHEALREVLGTHVEQKGSFVSDEALRFDFSHYQKVSAEELKTVELIVNKKIRNNAS
                     LQEYRELPVDEANKMGAMALFGEKYGEKVRVIQFGTSVELCGGSHVQSTGEIGMIKLV
                     HESSVAAGIRRIEAYTADKAEAYITAQLDVLTEVKQLFNNPTNVVGVITKFQQDTLQL
                     KKQLDGYVQEYVKQTKATLLTQVQQINGVDIIVFKSHDSAEICKDIAFQFKSQFANIP
                     FAFVAATQNDDKVSVSLLLSDNLVKKGLHASTIIKSIASDIDGSGGGQPFYATAGGKR
                     ASGIDTAVKKIIESITA"
     gene            complement(13798..14148)
                     /gene="rplT"
                     /locus_tag="PLJ82_03180"
     CDS             complement(13798..14148)
                     /gene="rplT"
                     /locus_tag="PLJ82_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOR40544.1"
                     /translation="MPRSVNHVASRARRKKILKLTRGYYGARKNVWTVAKNTWEKGLQ
                     YAYRDRKTKKRNFRALWIQRINAAARLEGMSYSKFMGALHKANIDINRKVLADLAMNQ
                     PEAFKAIVKKVQKA"
     gene            complement(14254..14451)
                     /gene="rpmI"
                     /locus_tag="PLJ82_03185"
     CDS             complement(14254..14451)
                     /gene="rpmI"
                     /locus_tag="PLJ82_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOR40545.1"
                     /translation="MPKMKTNSGAKKRFALTGSGKIKRKHAFKRHILTKKTTKAKRAL
                     TSFGLVDKTDESNVKFLLALK"
     gene            complement(14479..15036)
                     /gene="infC"
                     /locus_tag="PLJ82_03190"
     CDS             complement(14479..15036)
                     /gene="infC"
                     /locus_tag="PLJ82_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOR40546.1"
                     /translation="MAKVFTPRPNRNFQKKEEAHKTNEAIHHPEVRLVGENVEPGIYT
                     LAQAKHIADELNLDLVEISPNAVPPVCRVIDYTKFLYQQKKKEKELKAKTAKVTVKEI
                     RFGPQTDEHDYNFKLKHAIKFLEDGDKLKAFVFFKGRSILFKEQGEVLLLRFANDLEE
                     YAKVDQMPLLEGKKMIIMLSPKKKK"
     gene            complement(15148..15555)
                     /locus_tag="PLJ82_03195"
     CDS             complement(15148..15555)
                     /locus_tag="PLJ82_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HOR40547.1"
                     /translation="MKKLLITLFLAVSTVVFSFAQNELPEKARIFLETHFSNIQIQKV
                     SRVDIEQVQIFKVLLADKTQIEFDQTGAWTKINYKKSPKTISYLPETLQQEIESNKQI
                     KKVREIETDGYDIAIQLKNKKQVKGNRFGKLEK"
     gene            complement(15576..>15760)
                     /locus_tag="PLJ82_03200"
     CDS             complement(15576..>15760)
                     /locus_tag="PLJ82_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOR40548.1"
                     /translation="IRDNELKRIPYMVIVGEKEAENNELSVRKQGEGDKGTYSINDFA
                     QLIVDEVNNMTNKFLA"
BASE COUNT         4765 a         2668 c         2857 g         5470 t
ORIGIN      
        1 aaacttatgc aacacaaatt agtacaaagt acccaaacca agcattttac catttcttcc
       61 gattatccct tatgtatgga aatagatggc atctcactac ccgaaactta ccaagccacc
      121 atcagcatac tgcccaaggc tgtacggttt attgttgtgt agttattgtg tatggatatc
      181 cttgaacgag gtagatttta cctctgtagt tttactatat gctaccttta cactactttt
      241 aatcagacta atgtagaatt gaaatttact tctaaggcta atctattcgc agtaatctga
      301 gcttttaatc agactaatgt agaattgaaa taaatgacta aacgagggct tgtggttcgc
      361 tcctactttt aatcatacta atgtagaatt gaaagcaaat cacaacactt aaaataggag
      421 atacaaaaaa tgtttagttg tttatcaaat caaatataca atattttgaa ttgaaaatcg
      481 gcgtagcgaa agcaacttac aaccaaatat taaattgtta gaagtcgtac ttattaatgc
      541 aaattcttag aacctcattt ttgtagctaa aatctccttg taattactac acaaatcatc
      601 acactcttaa cttgaataaa ttaaaatttt tatatacatt tgcacacctt attctagtaa
      661 ctattcttat gaaaaaaatt accttacttc ttacattcct tctctttact tttgttgcct
      721 atagccaaaa tgatactatt tttttgaaaa gcggagaaat atatacagtt tatagagtac
      781 ttgatattga agatggctat ttgacgttta aacggaataa agattcaaac attagtacta
      841 ttgatgcctc attggtaaag accattggtt ttcaaccaaa acaactaaga gatgcaacag
      901 acgcgtatga taaggcgtat atcatcgaag ggagctttta tgaaaagaac aaaaaattat
      961 cagagtcagc ctataatgat gttggcacca atagcagaaa gacacgacac acatgattaa
     1021 taatgataaa gtttgcctga taacaggagc gagtagtggg attggattcg caattgcaaa
     1081 ggctttgcat gcggataatt ttgaggtaat tgcaactgca cgtagattga ataagctcac
     1141 agaattggga ttagattcaa atgacttatt agcaggagat ttgaatttgc cagaattcca
     1201 agattcgatt gtggattaca tattcgatag atatgggaaa tgtgatttct tatttaattg
     1261 cgctggaaca ttagaggttg gaactattga ggatatagac atagataaat tgacctcaat
     1321 gattcgatta aatgtagaga ctacatttcg attgacttat aatattctaa agcgatttaa
     1381 gaaacaagga tttggccatg ttattaatat ttcaagtgtt cttgggacaa aggtgagacc
     1441 aacagctgga ggatatgctg caactaaatt tgcaatggaa gcattatcgg aggctttgcg
     1501 aatggagttg acaggaacta atatcaagat tagttgtatt gagcccggat tggttatgac
     1561 agagttacat aacgattgga aagttcatcc taaagagagc atgaatattc acgacccctt
     1621 aactgttgac aatattgtgg ataccgtcag atttattatt aatcagccag accacgttag
     1681 gattcccaaa ttgatgatat tacccaaaga ccataatata taaaaagcta caggtgccaa
     1741 cacacggtaa aaaacattgc gcggaaagtg ctatatttga gtgatataac aattaataag
     1801 cggcgtaacg ggttgataga aaagtgctcc gaaaggcgca acgatttctt accgtcaaac
     1861 gcttggatac aacacaacct taataggttt tatacccaaa agcgttgtgc acaaatttgt
     1921 tatggattga gtttggcaac aagcatattg tattacacag gatcatttga caaagaattg
     1981 ctttacgtgt cagctggttt gagtatagca gctttagcaa ttgatttcca ttcgtacaag
     2041 tggattaaaa gggcttctat caaaccttcc ttgcaaagtt tatcggttca gttggagttt
     2101 tagaaacttc tttcaaaaag tagtgtaacc ttcctaaaat tgttcattca tctttttcca
     2161 taccacaact atggtataaa aaatactcgc tttgtgggat tgtggggtag tggaagtggc
     2221 tgtatctttg ctgtatcttt gctgtatata aatcactaat atataccatt atgaaactac
     2281 aagccatttg tcctatatcg tcgcaacaaa ttgacgagcg tattccacgc atcaacggtg
     2341 ctatcaccgt actattactc gtgttgtttg ctcttacaca gcattatttt attatcctgt
     2401 ttttggtagc tgattttttt gctcgagcca ttgaaaaacc cgattacagt attttggcaa
     2461 aattgtcaaa agcagtatta gctagatttt cgactacagg caagaaaaca aatgctggtc
     2521 caaagatatt tgcagcccgt ataggcttgg tatttagctt gtctatagca atattttcat
     2581 tgttcggtgt agattccgtt gctactgttt tggtggttat atttggtatt tgtgcttttt
     2641 tagaagctgc ctttaatttt tgtgtagcat gtaaaattta tccctttgtt tacaagttta
     2701 cccacttgaa cgaaatgtaa gtttgatttt taatagtagg ttgtgagaat tggattgtta
     2761 gcaataacag tccaattttt ttataaacgt aattcagaaa tagacagttc aatatcatta
     2821 cagtttaaat cacaaattgc taacgctaaa ggaacgtagt tctgatttct tcgtagaaaa
     2881 taatgatttt cgtcataata gaggaacgta gctctgatat ctatatgtat taaatcctaa
     2941 tttgtctcgc tttttttctg ctaacaacat gtataagaat ttattttata catggggctt
     3001 ttccccaaac cctaattact ttttttcccc gaactaaagt cggggtgtgc cacttgacac
     3061 aataaagtaa tcaaaaaagc tcaagactcc gcccacctcg ctctaaaaac cgacgctcag
     3121 cttgctaaaa cagtcccaac tcgctccttt gtcgctcaaa caggactatt tcttaacgcc
     3181 agcttcactt gtttttaggc tcatcggtcg aggtcagtcg gtaagtttga gagttgatgt
     3241 gcaaaattat actatcatgt aaagtggctt gaaaatccta ctactatcat aaaggtaaga
     3301 ttatcacctt atttatcaaa atacaatttt tccgaatgaa ccacaactta ttgtaacgta
     3361 aacacaaaca taaaaaataa caaaaccact gataataaat agattatcag tggttttgtt
     3421 tagttggtgg acctggaggg agtcgaaccc tcgtccaaac aagaaattaa tacgctttct
     3481 acatgcttat tttcgctttt attttcgtgt tgtagcaaga ccgaaaccac caactacaac
     3541 cttatcctct ttattttcac ttaccatacg aggcgatgat aagctatccc tgatttagtt
     3601 agcacctcta tatcaaaccg cctcaaggag tgggcatttg agagatgtct tgtttcagca
     3661 ccttgtaccg aaattaagct aatctactgt tattcgatta agcagcaaga gcgtagttat
     3721 tttcgccaat tagattgttg tatgttcaga ttaaagagct actcatacaa agctctgcat
     3781 gcttacatac caattttact cgctgtcaat tccagtcaag cccaatgtta tgtactactt
     3841 agcaacactt accgatctga tttccctgat aacggtaatt ttcacttgac cagggtaggt
     3901 catttcgtct tgaatgcgtt tcgcaatttc ttgcgaaagt ctttctgttg cttggtcatc
     3961 aattttatct gcgcctacaa ttacacgtag ctctctacct gcttgtatgg cgtaggtttt
     4021 cattacacct ggatacgata gggcaatatt ttctaagtcg tttaagcgtt tgatatacgc
     4081 ttctacaatt tcgcgtctag cacctggacg agcacccgaa atagcatcac acgcttgcac
     4141 aataggagcg agtagggttt gcatttctac ctcgtcgtgg tgagctccaa tggcattgca
     4201 aatatcgggt ttttctttgt atttctctgc aagtttcatg cctaaaagtg catgtggtaa
     4261 ttctggttcc tcgtctgcca ctttgcctat atcgtgcaaa agtcctgctc tacgtgcttt
     4321 tttcggattt aagcccaatt cggctgccat aacggcacat aaattagccg tttcgcgcga
     4381 gtgttgcaat aagttttgtc cgtaagacga acggtatttc attttaccaa tcaagcgaac
     4441 caattcagga tgcaatccat gcacacccat atcaatggtg gtacgtttac caatttctac
     4501 tatttcttct tcaatttgtt tggttacctt agctactacc tcttcgatac gagcagggtg
     4561 aatgcgtcca tctgttacca attggtgtaa cgataaacgt gcaatttcgc gtcttactgg
     4621 gtcaaatgct gatagtacaa tcgcctctgg ggtatcatct acaataattt ctactcctgt
     4681 agctgcctct aatgtgcgta tgtttctacc ttcgcgacca atgatacgac cttttaattc
     4741 gtcagattcg atatgaaata ccgtaactga attttcgatt gccgtttctg ttgccacacg
     4801 ttgtatggta tttaccacaa tttttttagc ttctttgttg gctgtcattt tggcttcgtc
     4861 cattatttcg ttaatgtacg aggctgctgt tgtttcggct tcattcttta acgattctat
     4921 taggttgttt cttgcttctt cggcagataa acccgcaaca gcttctaatt tttgtactgc
     4981 ttggtgatgc agtctatcta attcttcttc tttcttttca ttcaaactca actgattttc
     5041 atagtttagc tttatacttt ctagttccgc tgcttttttc tccagttgtt tttcctcatt
     5101 ctctataaac agctcttttt gcttaatttt agcctctatc gattgaattt tagcattacg
     5161 agtggtcatt tgtttttcat actctgcttt taattggatg tatttttctt ttacttccaa
     5221 tagcttactc tttttaagag tttcagcctc aatttgagcc gcatcaataa tgcttttggc
     5281 tcgtgctttt tgcactattt gcaccactat tgccgatacc gtagcaccgc caacaaatcc
     5341 cagaatgata taaataattg tcatttataa taggtattag taaataaata aaaacattgc
     5401 aaaaacaaaa acaacagagt tgattttgag aattgcaatg ttataaaatc tgcgtaaaaa
     5461 actgaatagt atattattcc tcgtttttag tgtctagttg attgtttttc aggtattcag
     5521 ccaatcgatt atccaatgct tgaatggtag atataaatgg agaattatct ctttcttctt
     5581 ccaatctcaa actaagtaac gataattcaa aagctaccat tatcacatag tcaatgtcat
     5641 taaggtttct atcgaacttt ttacgataag ctaaaattct ttcattcaat tgtttagctg
     5701 cttttcgata tatttcttct tcttctcttc taattttgat aggataagaa gtgtccaata
     5761 tcattactcg aatggtgagt aagtcttgtt tttccatatc aatcattgag cagtgctatg
     5821 caattgtcaa tttcacgcat cattttcgac aaccgtaact ttccatcttt gatgtctgtt
     5881 ttagaaagag caactgtttt tgcaattttc aggttgtcgt atttttcttg caattctact
     5941 attgtcttgt gagcatgcat aagttcctta tttttacggt cataattatc cttgagggtt
     6001 gcaagttgag attgatacga atccattttt tgcaacaaca tagaaatacg actctcaaaa
     6061 ctttttagta acttatcgtg ctcttcagtc atttttgtaa aatctataca aaaatagaat
     6121 attttgaatg aaaaaaaaaa tattttgtga ttttttacat aaacaagact ctctaattgc
     6181 taaaaaaggc atcaactata tatggttgat gcctttttat gtgtattatg tgtaaaaaaa
     6241 ttatctttta cgttctttga ttcttgcttt tttacctgtt aaagctctta ggtaatataa
     6301 ttttgctcta cgaactttac catttttatt tacctctatt gcattgataa aaggagagtc
     6361 cattggaaaa attctttcaa cacctacgtt ttcagatact ttgcgtacag taaaacgttt
     6421 ttttacgcca tgtcctacta tttttataac aacacctctg tattgttgca cacgttcttt
     6481 gtttccttcg ataattttgt aagaaacagt aattgtgtct ccacttttaa aagcgggtaa
     6541 ttctttttct gaagcaaatg acgcttcggc taccttaagt aaatccattt ttgctaattt
     6601 attaaattca acattacgac ataatattac agacccccat gttcgtgcaa gagattatgc
     6661 caaaaacgac tgcaaagata gcattagttt tttatcttac aaactgttgc atgtttattt
     6721 tacacaattt gtttttattc aaaattttgc gttactttgt aattgtcgct tactttttta
     6781 tttcgaaata taatgaaaca taactagcat gtcaaaatta tacatattat tggtattcgt
     6841 actaggcgtt ggtttggcta cttatttcgt aaggcaacca tcacagcaaa ttactattca
     6901 ttcagaagct attttggtaa atgctgatgt ggggctatcg ccgtgtgttg ctattgatga
     6961 gtttgtgcaa ccatttaaag ataccattga tagtgagatg aatacggtaa ttgggtatgc
     7021 ccctcaagat atgactgttg gcaggcccca gagtttgttg tctaattttg tagccgattt
     7081 attgctcgag cgtgctaata ccattattcc tacggctatt tctgttgtca atatgggagg
     7141 tttgcgtaat tcaataaaaa agggcgatgt tacagttcgt tcggtgtttg cggttatgcc
     7201 ttttgaaaat gaatgtgtta tcgttgaaat gagtggagag gctatttggc aattggctaa
     7261 cgaaatagta gcggtaaagg gtgaaggatt tggtgggatg caagtagttg tgaaaaataa
     7321 tacattgcaa caagcgtatg ttcagggaaa acttcttgat agaaaagctt cttatcgtgt
     7381 agtaacgagt gattatttac taacagggaa ggatgggtta acggcattgt tgcaacataa
     7441 agtattggtg aatcctgcta ttaaaatgag agatgttata ttggagcaca ttgtaaatga
     7501 aacaaaacaa ggaaagccca ttattgcaga aatggataat cgttatcaaa ttgaataaag
     7561 ttatgaaaca aaggacatat atattccccc tgtttatatt aatcagtata cttttttcgt
     7621 gtacttctgg cgagcgaaaa ctcgtcattt tgcataccaa tgatgtacat agtgctattg
     7681 agccaaatga aaaaggcgag ggtggtgttg ctatgcgttg tgcaattatt gatagtatta
     7741 ggcagagtga aggagatatt ttgttggttg atgctgggga ttattttcag ggaactccct
     7801 attttaattt ttttggagga gccgtagaaa ttgaagctat gaataacatg ggctatgatg
     7861 tgataacgct tggcaaccat gagtttgata atggcgttga ggctttggtc aaacaactta
     7921 catcatcaaa agctaccatt gtatctacta attatgatgt ttcagaaaca gctttaaaag
     7981 ggattgtttt tccgtataca attactgaaa aaaatggtat taaagttggt atatttggct
     8041 tgggagttag tccagaaggg ttgatacgca atgatttatt tgggaaaata cattataaag
     8101 atcctattaa tagtgctaat aaaatggctt ttaacttaaa gaaaaatgaa aagtgtgatg
     8161 tggttgtgtg cttatcgcac ttaggatata ggtatacaaa caatacggaa aaagtatgtg
     8221 attctattct tgcgatacgt tctgcagata ttgatgtaat tattggggga catactcatg
     8281 atttgatagt aaataaaaaa ataaaaaata aaatgggcaa agaagttaca gttgcacaaa
     8341 tgggaaaaaa tggtaggtat gtgggggaaa ttaaaatcag tattaactaa aaaaagctcc
     8401 gattagaagc tttttttagt gtgtatattc tttatttttt aaatcgatac aattttacga
     8461 gtattttctt aatagttgta ttctccatat aagaagagaa tttatcagca tctttgccat
     8521 aagcaaataa aggaacagga acgggactgt gataactgct tgtaaatata gtctgtaatt
     8581 tttttgtatc tgtatcctta cctagtattt gtaatccacc tgtttcgtgg tctgctgtta
     8641 caataactaa cgtttcttta tttttatctg caaaatccat tgcaacacct acagctttgt
     8701 cgaaatctag cgtttcttcg ataatgccat caatatcatt tgcatgtcct gcccagtcta
     8761 tctgagatcc ttcaataagt aagaaaaatc cttttttatt tttactaagc acatctaagg
     8821 ctaattgtgt tccttcagaa agcatatttc ctcttccttc aaacttgctt gtcattccaa
     8881 cgtctgctaa taacacgcct tttttgttgg agtcggatat agaggcttct tttattgaag
     8941 aatatacccc ataccctttt gcatgtaaac tgtcaacaag gttttgttta tcttctcttt
     9001 cgctaaaaaa ttttcgaccg ccaccaatga aaacatctac tcccgagtgt agaatgtcaa
     9061 cagctatttc ttcttccatt tttctgttca cttggtgtgc taaaaaactt gcaggggttg
     9121 catgtgttac agagcttgta actacaacgc ctgtacttaa tttattggta tgagcaatgc
     9181 gtaggataga tggtattgct acgctatctg gattcattcc aatcatgccg ttttttgttt
     9241 tcacaccaca ggcaagagcc gtgccacctg ccgccgaatc agtaatgtaa tgatcagcag
     9301 aatgggtgtc tacaagtcca atataatggg ctcgttgcat atttaacgca ctgtcggcaa
     9361 ctatgctaga ggcatgaatt tgatttaaac ccataccatc tccaattaag aaaatcacat
     9421 tttttaccct agcaaacgtg ctaattggga tgataatgct gataataaat acaataaata
     9481 ggttttttct catggttttt gtttgttttt attcaattaa gtcatcaagc tcagttttta
     9541 tattgtgtag ttgttttttt attttttgta gggtatcaat aatttcaaaa ttttttttag
     9601 ttgatgcttt gttctctttt aagcgtttct ttattccatc taatgttaat cctttttctt
     9661 cttttaaatg tttgatgagt aagaattcgt caatgtgttc tcgcgtataa aaacttgttc
     9721 ctttatcgtt ttttcgaggc ttaatcttat caaattgctg ttcccagaaa cgtatagcag
     9781 aaatattaac atccactaat tcagctactt cttttatgga gaagtatagt ttttgtttgc
     9841 tttcttgtat gttgttagta gcttcttcca ttagtagagt taatcaaata cacgtccata
     9901 attagcagat atttgtatca tttgatcata ttcttctgga gataaatcca taaaataata
     9961 gttttgagga ttttgaggtt ttcctttcaa gcgtacctca taatgtaggt gaggacctgt
    10021 tgatttacct gtatttccta caaatccaat tacttcacct ctacttacct tactgcctct
    10081 acgtagccct tttttgagtt ggtgcatatg tgcataaaca gtaacatagc caaaaccatg
    10141 gtcgataaat acagcatttc catatccttg ttcccatcca gctttgataa cagtaccatt
    10201 gccagtagca aaaatttttg tacctgttgg tgccgaaaaa tccattcctt tatgaaatct
    10261 acgtgtatga taaattgggt caattcgcca tccatagcca gaagctgtcc tttttaaatc
    10321 cttgttagga atgggttgta tagctggaat atgttgtagc tttttttctt cttgtttagc
    10381 taatgctaca agttcgtcaa aagaattaga ttgaatatat aacattcttg ataccatatc
    10441 cattttctga ctggtagcaa tgactaaatc agacgttcgc attctaagaa gtttttggta
    10501 ccgactgctg ttgatataat ttctacgtcg gatactcgaa ggaatagaat ccacttggag
    10561 tactactcga taaaaattgt catcacgttg ttgaatattt gttagcactt ccatagcatc
    10621 atctaattgc ctagaaagta actcgtattg tgtgctaata cgtttatttt cagcttctag
    10681 taacttttca ttaggagatt tgaccgtaaa aatgaagata acaaaaaaaa cgatgcccat
    10741 taaaatactt ccgattaaat attgtccaat tttaatcaga cggtgcctaa ttgtattgtc
    10801 tattcgatcg tagttaaggg tgtgttcgtt aaaatgatat tttgcttttc ccatgattgt
    10861 tgtttcctaa aaccaatctg tttattacac aggacaaaca gattaacaaa agtagtttga
    10921 aaattcattt aagacaacaa aaataacaaa aaaaatacta attttgtggc ttgaaatctg
    10981 aatttgcaaa ttattttaga taccaatatc taactgcttg gtataattta gtttgtatac
    11041 tagatacgta tgtttgattt gataaatttt aattgaaaaa taataatgaa atctgccaaa
    11101 gaaattagag aatcgtttac atcctttttt gcatccaaag gacataagat tgttccgtca
    11161 gcacccatgg taattaagaa tgatccaaca ctcatgttta caaatgcggg gatgaaccaa
    11221 tttaaagata ttattttggg aaatgctaca gtcgtatatc ctcgtgtagc aaattcgcaa
    11281 aaatgtttgc gtgtaagtgg caagcacaac gatttggaag aggtagggca cgatacttac
    11341 caccacacca tgtttgaaat gttgggtaat tggagttttg gcgattactt taagaaggat
    11401 gcaattgatt gggcatggga gtacctagta gaggtgttaa aaattaatcc agaaatattg
    11461 tacgttactg tttttgaggg caatccagcc gaaggcttag aaagagataa cgaggcagca
    11521 gcatattggg cacaatattt accccaagac cgtattatta atggtagtca aaaagataat
    11581 ttttgggaaa tgggagatac tggtccatgt ggtccatgtt ccgaaataca tgttgattta
    11641 cgttcagcag aagaaaaact aaaagtacct ggtgtaaaat tagtaaatga aagtcatcca
    11701 caagtgatag aaatttggaa tcttgtgttt atgcagttta atcgcaaagc agacggttcg
    11761 ttggaattat tacctgccaa acacgtagat actggcatgg gatttgaacg tttgtgtatg
    11821 gcaatacaag gaaaaacatc gaattacgat acggatgtat ttcaacctat aattaaagca
    11881 atggctacac tagctggggt ttcttatggg gaaaataacg aacaagatat tgcattgcgt
    11941 gtaatagccg accatattcg tacgattgct ttttcgatta ccgatggtca gttgccttct
    12001 aatgcaaaag cagggtatgt tattcgtcgt attttgcgta gagcggtgcg atatggatat
    12061 acgtttttaa atcaacgttc atcgtttatg catgcattac ttccagcact tattgatagt
    12121 atgggaagtg cttatccaga attagaagca caaaaagggt tgatagagaa agtgattaaa
    12181 gaggaagaag aatcattttt acgtacctta gaaacaggta tccgtttgct cgataaagta
    12241 attactgaag ccaaacttgc aggtaaaaca accataagtg gtaaagatgc ttttgtattg
    12301 tacgatacgt atggttttcc tcttgattta actgaattga tagccaagga aaataacgta
    12361 atggtagatg tagcagagtt tgaacaagaa atgcaaaaac aacgcgaaag agccagaaat
    12421 gcagccagtg tagaaacggg tgattggatt gtagttcgcg atggagaatc agtatttgat
    12481 gggtatgatt ttgctgaaat ggatgtacaa ttgcttcgtt accgtaaaat ggtacaaaag
    12541 ggaaaatatc tataccaatt agttttcgat cgaacaccgt tttatggaga aatgggcggt
    12601 caagtaggag atactggcta tattcttgca gatactaaaa aaattgccat tgttgatact
    12661 aaaaaggaaa atgggttaac ggtacatatt acattagaat taccagaaac tattgatgct
    12721 acctatacag cagtggtaaa tgtggaaaaa cgcaaaagaa ttgcggctaa ccatacagca
    12781 acccatttac ttcacgaggc attacgcgag gtattgggta cacatgtaga acaaaaaggt
    12841 tcgtttgtgt cggatgaagc attgcgtttt gatttttcgc attaccaaaa agtaagtgcc
    12901 gaagaattaa aaacagtaga attgatcgta aataaaaaga ttcgcaataa tgcatctctg
    12961 caagaatata gagaattacc tgttgatgaa gcaaacaaaa tgggagcaat ggcattgttt
    13021 ggcgaaaaat atggcgaaaa agtacgtgtt atccagtttg gcacttcggt agaattatgc
    13081 ggtggttcgc acgtacaatc aactggagaa atcggaatga ttaaacttgt gcacgaaagt
    13141 tcggtagcag caggtattcg tcgtatagaa gcctatacag cagacaaagc agaagcgtat
    13201 attacagctc aattagatgt acttacagaa gttaaacagt tatttaataa ccctacaaat
    13261 gtggtaggtg taatcacaaa atttcagcaa gacacacttc aattaaaaaa acagttggat
    13321 ggctatgtgc aagagtatgt aaaacagact aaagcaacgt tattaactca ggtacagcaa
    13381 attaatggcg tggatattat tgtgtttaag tcgcatgatt cggcagaaat ttgcaaagat
    13441 atagcctttc agtttaaatc acagtttgca aacatcccat ttgcttttgt agcagcgaca
    13501 caaaacgatg acaaggtaag tgtatcgttg ttgttatcag ataatttagt aaaaaaagga
    13561 ttacatgctt ctacaatcat taaatcgata gcatcagata ttgatggaag tggaggaggg
    13621 caaccatttt acgcaacggc aggaggaaaa agggcttcgg gtatagatac ggctgtaaaa
    13681 aagattattg aatcgataac tgcttagtag gagtaaacga tataactaaa aagaggaaca
    13741 atttattttg ttcctctttt tagttatata aagtagtaaa actttattct aattacacta
    13801 cgctttttgc acttttttta caatagcttt gaatgcttct ggttggttca tagctaaatc
    13861 ggcaagtacc ttgcggttga tgtcgatgtt tgctttgtgt agagctccca taaatttaga
    13921 ataagacatt ccttctaatc tagcagctgc atttatacgt tgaatccata atgcacggaa
    13981 gttacgtttt ttggttttac ggtcacgata cgcatactgt aacccttttt cccaggtatt
    14041 tttggctact gtccatacgt tttttctagc accgtagtaa cctctggtta gttttaagat
    14101 tttttttctt cttgctctcg aagcaacatg attgactgat cttggcattt tcttctgttt
    14161 ttttgaatgg tagcgtcact aataggtgat ctttcggcta ttcattcggt taatactgaa
    14221 ttgatttaca aataaaataa actttgacgt agactattta agtgctaaca aaaatttaac
    14281 attgctttcg tctgttttgt caacaagtcc gaaactagtt aaggctcttt ttgctttggt
    14341 agttttcttc gtcaagatgt gccttttaaa tgcatgtttt ctcttgattt ttcctgatcc
    14401 ggtaagggca aacctctttt tggcaccgga gttagttttc atttttggca ttacgttgta
    14461 tatttaaaaa ttaaaaaatt attttttctt tttgggcgaa agcataatga tcattttttt
    14521 tccttctaat aaaggcattt gatccacttt tgcgtattct tctaagtcgt tggcaaaacg
    14581 tagtagcaag acttctcctt gttctttaaa taaaattgaa cgacctttaa aaaatacaaa
    14641 agcctttagt ttatcaccat cttctaaaaa tttaatagca tgcttcaatt taaaattata
    14701 atcatgttcg tcagtctgtg gaccgaaacg aatctctttg actgtaactt tagccgtttt
    14761 agcttttagt tctttctcct tctttttttg ttggtacaag aattttgtat agtcaattac
    14821 acgacaaact ggcggtactg cattaggcga aatctcaact aaatcaagat taagttcatc
    14881 cgctatatgc tttgcttgtg ctaatgtgta gataccgggc tctacgtttt ctcctactag
    14941 acgaacttca ggatgatgaa tagcctcatt ggttttgtgg gcttcttctt ttttctgaaa
    15001 gtttctattg ggtctcggtg taaaaacttt tgctatagta aacgtcctcc taaattaaat
    15061 aattactaaa tgcttatttt tcagcccgca aatataggct ttttttatga tatatcacaa
    15121 tttatcgtta aaaaaatagt ggttttttta tttttcaagt ttaccgaatc tatttccttt
    15181 cacttgtttt ttatttttaa gttggatagc tatatcatat ccatctgttt ctatttctct
    15241 tacctttttt atttgcttat tgctctctat ttcttgttgt agagtttctg gtaagtatga
    15301 aatagttttc ggtgattttt tgtaattaat cttggtccaa gctcctgttt ggtcgaattc
    15361 tatttgtgtt ttatctgcta ataaaacctt gaatatttgt acttgttcta tgtcaacgcg
    15421 acttactttt tgaatttgga tattgctaaa atgagtctct agaaaaatac gtgctttttc
    15481 aggtaattca ttttgagcaa aactaaaaac tacagtactt actgcaagga atagggttat
    15541 tagtaatttt ttcatgagtt cagtctatta attgattatg ctaaaaattt gttagtcatg
    15601 ttatttactt catctactat tagctgtgca aaatcgttga tggaataggt tcctttatcg
    15661 ccttcgcctt gtttgcgtac agatagttcg ttgttttcag cttctttctc gccaacgatt
    15721 accatatacg gtatgcgttt taattcgttg tcacgaattt
//
LOCUS       DAOSRP010000031        15722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_3846_length_15722_cov_5.129189, whole genome shotgun sequence.
ACCESSION   DAOSRP010000031 DAOSRP010000000
VERSION     DAOSRP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15722
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3846_length_15722_cov_5.129189"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLJ82_03205"
     CDS             <1..478
                     /locus_tag="PLJ82_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOR40549.1"
                     /translation="TATLNLCERTCTQEIVRTVDTTGHVFAGTPADITYTCAGDVPVA
                     NTAAVTVTVANARGCETTVPFIVNRALDIIPALFFSPNNDGVNDTWEIENIGHYPHAI
                     IEIYDRFSKLLITYKGNETGWDGMYLGYPMPMTDYWYIITIPELGKPRTGHFTLKR"
     gene            complement(536..2815)
                     /locus_tag="PLJ82_03210"
     CDS             complement(536..2815)
                     /locus_tag="PLJ82_03210"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOR40550.1"
                     /translation="MAKVNKEDALRYHAEGKPGKIEVVPTKPYSSQRDLSLAYSPGVS
                     EPCLEIEKDASKAYDYTAKGNLVAVISNGTAVLGLGDIGAIAGKPVMEGKGLLFKIFA
                     GIDVFDIEVNEKDPKKFIEVVKAIAPTFGGINLEDIKAPECFEIEDALKRDLDIPIMH
                     DDQHGTAIISAAGLINALELTNKNIEDVKIVVNGAGAAANSCASLYIQLGAKLENIVM
                     LDSKGVINKQRTDLNDRKKPFATDRTIKTLTEALAGADVFLGLSVEGVLSADMIRTMN
                     QNPIVFALANPNPEISYEEAMSSREDIIFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TKINDEMKVAAVKAIAELTKRSVPDVVSAAYNLKKISFGREYIIPKPLDPRLLTIVAP
                     AVAKAAIESGVARKTIDNWEAYDAKLKELMGRDNKILRYIYETARQQPKRVIFSEANH
                     INMLKAAEASLKEGICFPVLLGNEEKIARIAAENHINLEGIEIINLRHDREENRRIEF
                     AQQLTAKRYRQGMTFEDAYEKMYERNYFGMMMVEMGQADALITGVYSKYADTIQVAKD
                     VIGFREGLNHIGAMHILNTKKGIYFFADTLINRNPTHETLKDIVKLTHSSVQLFGYDP
                     VMAMVSYTNFGAEPTGSPAAINKVVQEIHNEHPEIVIDGEMQVNFALNTELRDKKYPF
                     SKLTGKKVNTLIFPSLSSSNSAYKLLLEMGMADSIGPIQMGLKKPIHILDIESSVRDI
                     VNVTAVAVIDALMEEKKKQ"
     gene            complement(2977..3957)
                     /locus_tag="PLJ82_03215"
     CDS             complement(2977..3957)
                     /locus_tag="PLJ82_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOR40551.1"
                     /translation="MKAITQNSIGNAETMYIGEVDIPVLRENEVMVQVKAFGVNRADI
                     LQRKGKYPSPAGASPLMGLEVAGIVAEAPVSSPWKIGDKVFGLIPGGGYASYVAIDAE
                     MLWRMPDTMSFEEAAAIPEAFLTAFLALQWQGKLQRGQKVLLHAASGGVGTAAIQLAN
                     AMQAEIVVTASASKHAICAKLGATVCIDYKTESWVDVVQEVMGKNSIDVVVDFLGASY
                     FNDNISVLAMDGRMVMLALMGGADVSGVDLRKIIGKRITVIGSTLRNRPLSFQRKLAR
                     EFAEQFLPSFSDGRLQPIIDTVMDWTLIKEAHERMESNQHVGKIVMKVSE"
     gene            complement(4083..5291)
                     /locus_tag="PLJ82_03220"
     CDS             complement(4083..5291)
                     /locus_tag="PLJ82_03220"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOR40552.1"
                     /translation="MSSNQKATAWVVTSSAMGINLILGLLYSWSVIKAALVAEGWTNT
                     EASLPYTVCVALLAFMTVFGGKLQDKFGPRVVALGGGILFGVGIFLSAFVHTPWMMVL
                     TFGVVAGLGMGFAYSAATPAAIKWFEPRKKGLIAGIVVAGIGISSIYMAPLTNYLLQG
                     NTVQGTFMILGIIAIVFLSIFGAILHNPPANYIPKAKEGAKVVAHSADFTWQAMMKRK
                     PFYLLWATYFLSATAGLMLIGHIVSIARVQTGNPELNAFWLVMLFAAFNTSGRVLGGF
                     LSDKMGRTTGLLIVFLLQAINMFVFSFYTSFPLLVVGFAVAGIAYGALFALFPAVTAD
                     FFGVKNLGVNYGIIFTSWGIAGIVGPILGGMVADITGTYAGSYMVAGIMLLLGALLVK
                     LIKTPQQEIK"
     gene            5567..6376
                     /gene="zupT"
                     /locus_tag="PLJ82_03225"
     CDS             5567..6376
                     /gene="zupT"
                     /locus_tag="PLJ82_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOR40553.1"
                     /translation="MIDEKYLFAFALTLFAGLATGIGSLMAFFSKKFNPNFLSAALGF
                     SAGVMIYVSFVEIFFKAKESLTASLGDVTGYWMTVVSFFAGIALIALIDKLVPSGENP
                     HEISNIEEANYKNSTSNDKLLRMGLFSALAIAIHNFPEGLATFTAALTDPTLGISIAV
                     AIAIHNIPEGVAVSVPIYYATKNKKKAFWMSLLSGLAEPVGAIIGFIILRPFLDGATL
                     GIIFGAVAGIMVYISLDELLPTAREYGKHHIAIGGLIAGMAVMSVSLLLFA"
     gene            6388..7350
                     /locus_tag="PLJ82_03230"
     CDS             6388..7350
                     /locus_tag="PLJ82_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOR40554.1"
                     /translation="MKLRCVRYTLFLFFFLVFTACGSLVVKKQEKISLYTHTTLIDGQ
                     NRFYKVYVPSHIKNNQTIPLVIALHGNGGNAEALIRETKWHTKASSEGFLVVAPEASR
                     PNPNKPRNQRDNRQTWNDGSGRFHSGERSIDDVKFITTMINELKNTYNIDKNKIFVTG
                     FSNGASMTFRLGMELDCIKAIAPVAGVNWIKNNTSNRKISLLYIIGAQDRAKPLEGGI
                     TKTANGIVLEKTPKPPIYENIKRWATFIDCIGEPTTFTLAQGVSGLRFTDCSSNTSIE
                     YIIVDDLGHIWPGARQIIPKSIVGNASEKLNATEYIWNFFNTAK"
     gene            7356..8258
                     /locus_tag="PLJ82_03235"
     CDS             7356..8258
                     /locus_tag="PLJ82_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR40555.1"
                     /translation="MNTENITLAFNKIITDIVNWLLYDFTYILISLVVVIVLIWLLNA
                     ISRNLEKVILKRSDALHDKEAQKRVKTLMSIGRGIMRLILWAIFALILLKRIGIEIGP
                     IIASAGIAGIAIGFGAQELIRDFLAGFFVLLDNQIRADDIVVINGTTGVVEKIELRTI
                     RLRDHSGVVHIFQHGKVNTLSNMTKEWSALVFEIGVAYKEDVDTVIAIMKSVGEDLEK
                     DSRFSGNILEPISILGVEQFANSSIQLKVRIKTQTGEQWQTRREYMRRLKYAFDKKGI
                     EIPFPHTTLYWGEKSKPMDISLQK"
     gene            complement(8247..8783)
                     /locus_tag="PLJ82_03240"
     CDS             complement(8247..8783)
                     /locus_tag="PLJ82_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOR40556.1"
                     /translation="MNPYVILQKYYSSNEEAYDILVNHSESVANKALSIAHNHPELKI
                     DTEFVFQAAMLHDIGIHLTNAPSVACYGSYPYVCHGYLGSGILMEEQLPQHALVCERH
                     TGTGISLEDIVTRKLPLPLRDMKPISLEEQLICFADLFFSKTHLEEELSITRIETKLQ
                     KFSSHSVVQFHQWCDLFL"
     gene            complement(8783..10399)
                     /locus_tag="PLJ82_03245"
     CDS             complement(8783..10399)
                     /locus_tag="PLJ82_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR40557.1"
                     /translation="MEKHKLLYPIIIGISIAVGILLANLYYQQAEQTTGFAIPKKVQG
                     NKFDEIMYLLDNNYVDVIDKNALTEEFIPQILKFLDPHSAYLTPEEVAESATELQGSF
                     SGIGVQFNIQNDTVMIVNVIPGGPSEKLGILPGDRIIAVNDSSFVGADVTNATVLKTL
                     RGEKGSIVKVRIKRNGSPLPLDFDIVRGDVPVKSIDVVYVIADSTGYIKISNFGANTY
                     REFLDALVILREQECNNIIVDLRNNSGGYLSAVIQMTNEFLKRGQLIVYTEGVNQARE
                     DAIADGRGNFRKENICVLINEWSASASEIFAGAIQDHDRGWVIGRRSFGKGLVQQQIP
                     LSDGSELRLTVARYHTPSGRSIQKPYKSGESDENYDVEIEKRVLHGEMFQKDSISFAD
                     SLKYYTAKGRTVYANGGIMPDIFVPHDTTYYTPYYTNVLNNGIVYDFSFAFVDKHRAK
                     LSTYDTWEKLVEYLVKKTTYFSEFIRYAEQKGVSRNSQEIAKSKALLANLLQAYIARN
                     LLGDEAFYPILNLQDNTVKKAIEMVGKPIQ"
     gene            complement(10403..10828)
                     /locus_tag="PLJ82_03250"
     CDS             complement(10403..10828)
                     /locus_tag="PLJ82_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOR40558.1"
                     /translation="MNTKQLVLDKRYMQMALIWAENSYCKRRQVGAILVKDKMIISDG
                     YNGTPCGFENICEDESFKTKPYVLHAEANAITKIARSHNSSEGATLYVTTSPCIECAK
                     LIIQSGIVRVVYANEYHVTDGVDLLVRAGVEVLKIDLDN"
     gene            complement(10867..12009)
                     /locus_tag="PLJ82_03255"
     CDS             complement(10867..12009)
                     /locus_tag="PLJ82_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR40559.1"
                     /translation="MKQSILFLFVVFVCLLSSCSQTEEYTIQGTVSGVETDTLFLEKV
                     ELGKIEIVAYQVLKKNGKFSFSFPRAVEPHYVRLRLQQGVLYLVADSTETISVYSNKQ
                     TFGQADSIVGSAASKRMQSIVHYVAQTQRKMTASQDKYQRGKIVAQDFTEETTQLVRQ
                     LKDFLRKEVYQDTRSSVAYFALYQQINGYYVFNPYDKEDVKVYAAVATPLQVHYPQTA
                     RTKNIEDVVLKGMQRRKNAEDKLVREIVGKGFIDISLSTVQGVERSLSASLGRATIVD
                     FSLFQSENSYAHTVLLQELYRVYASKGLRIYQVSFDDDAVFSVVAKQLPYTVVHDKKG
                     LVSPLLQTYNISTLPTVFLLDKEGDIVARNVSLPLLEQSIQQLLEK"
     gene            12168..13190
                     /locus_tag="PLJ82_03260"
     CDS             12168..13190
                     /locus_tag="PLJ82_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOR40560.1"
                     /translation="MNTAFRLTRFSLFVLIVLSTMSCRNEQKQFFINEGLVYGTYYRT
                     TYEHYQDIEVDIKEAMAKVGTSLSTFDSLSVISRINRNDSTVVADTYFTEVFTTAQQV
                     STHTGGAFDITVAPLVNAWGFGFDPQHERTAILIDSIKQWVGYNTISLQNGKILKQHP
                     QTQLDASAIAKGYACDVVAQVLREAGIENFLVDIGGELVVKGHNKRNTDWQVGINKAI
                     DDATQQNQEIQQIISVTDVAIATSGNYRQFYYENNKKIAHTIDPRTGYPTNHSLLSTT
                     VIAPTCMLADAYATAFMVMGNWEEIMKVVNNAVPPIAVFCIFEDTTGKHIEMASPGFE
                     QYLQEK"
     gene            complement(13401..14147)
                     /gene="fabG"
                     /locus_tag="PLJ82_03265"
     CDS             complement(13401..14147)
                     /gene="fabG"
                     /locus_tag="PLJ82_03265"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOR40561.1"
                     /translation="MSLLKGKVAVVTGAARGIGKAIALKYASEGADIAITDLAVDDNA
                     KAVEKELAAYGVKAKAYASNAANFDDTHNVVSEILKEFGRIDILVNNAGITRDGLMMR
                     MTEQQWDMVINVNLKSAFNFVHAITPIMMKQKTGSIINMSSVVGVSGNAGQCNYSASK
                     AGMIGLTKSIAKELGSRGVRANAIAPGFIITEMTAALSDEVRDQWAAQIPLRRGGTPE
                     DVANVATFLASDMSSYVSGQTIHCCGGMNM"
     gene            complement(14351..14866)
                     /locus_tag="PLJ82_03270"
     CDS             complement(14351..14866)
                     /locus_tag="PLJ82_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40562.1"
                     /translation="MVTFSGIIPVETQWLTIQVVGNGSVKVGDVAYTTAVPVAKDEQV
                     TISAIPNHGNVFVAWSGDLVSTTASETITMTTDKTITVTFDQVTQLTQNEAVSVVLYP
                     SPFTDQLHVQSAEKLDKILVTNAVGVSMYDTTNVDSAITIPTSQWTSGIYIVHMYKAN
                     KVIATKKVVKF"
     gene            complement(14915..15073)
                     /locus_tag="PLJ82_03275"
                     /pseudo
     CDS             complement(14915..15073)
                     /locus_tag="PLJ82_03275"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
     gene            complement(15110..15325)
                     /locus_tag="PLJ82_03280"
     CDS             complement(15110..15325)
                     /locus_tag="PLJ82_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40563.1"
                     /translation="MQHATFFPVVTDSLLPYIDSFSYVSFDSNDSILVPPLGFPVILF
                     CFSPHNSFYTHNEFKNESVVVGQMTRW"
     gene            15382..>15722
                     /gene="folB"
                     /locus_tag="PLJ82_03285"
     CDS             15382..>15722
                     /gene="folB"
                     /locus_tag="PLJ82_03285"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOR40564.1"
                     /translation="MAIINLENMEFWSYHGCFEEERRIGTKYVISVSLAVDMQQAMAS
                     DNLHDTINYQEVYSIIKEEMDIPSNLIEHVAGRIQHRLKERFPQSTQQTISLSKFNPP
                     LGGQIEKVTIV"
BASE COUNT         5034 a         3134 c         2596 g         4958 t
ORIGIN      
        1 tacagctaca ctcaatttat gtgaaagaac ttgtacacag gagattgtgc ggactgtgga
       61 tactactgga catgtttttg ctggaactcc tgctgatatc acttatacct gtgctggcga
      121 tgttcccgtg gcaaatacag ctgctgtaac ggttactgta gccaatgctc gtggctgcga
      181 aacaacggtt ccgtttatcg taaatagagc tttagacatc attcctgctt tattcttttc
      241 tccaaataat gatggggtaa acgatacatg ggaaatagaa aatattggtc attacccaca
      301 cgccattatt gaaatttacg accgatttag taaattgcta ataacgtata aaggaaacga
      361 aactggttgg gatggaatgt atttaggtta tccaatgcca atgacagatt attggtatat
      421 tattaccata cccgagttgg gcaaaccccg cactggtcac tttacgttaa aaagataaat
      481 atacaaagaa agcaggtaaa ataacctgct ttctttcgtt aaaaccatat ttgtattatt
      541 gtttcttttt ctcttccatc aaggcatcaa tcactgctac tgctgttaca ttaacgatat
      601 ctcttaccga actttcaata tccaaaatat gaataggttt tttaagcccc atttgaatag
      661 gaccaatact gtcagccatg cccatttcga gcaataactt gtatgcactg tttgacgaac
      721 ttaaacttgg aaaaatcaag gtatttactt ttttcccagt cagttttgag aaaggatatt
      781 ttttatcgcg taattctgta tttaaggcaa agttcacttg catttcgcca tcaataacta
      841 tttcaggatg ttcattgtga atttcctgta ctactttatt gattgcagct ggacttcctg
      901 ttggttctgc accaaagtta gtatacgaaa ccattgccat gacaggatcg tatccaaata
      961 gttgaactga actgtgtgtt agttttacaa tatcttttaa tgtttcatgc gttggattgc
     1021 gattaatgag tgtatctgca aaaaaataaa tccccttttt tgtgttcaaa atatgcatag
     1081 caccaatatg atttaatcct tcgcgaaaac cgatcacatc tttagcgact tgaatagtat
     1141 ccgcatactt tgaatatacg cctgtaatca aagcatcagc ctgacccatc tcaaccatca
     1201 tcataccaaa atagtttctt tcatacattt tttcgtatgc gtcttcaaac gtcatccctt
     1261 gacgataacg tttggctgtt aactgttgag caaactcaat gcgtcgattt tcctccctat
     1321 cgtgacgtaa attaataatc tcaataccct ctaaattaat atgattctca gcagcaatgc
     1381 gtgcaatttt ttcttcattt cccaataata caggaaaaca gataccttct ttgagtgatg
     1441 cttctgcagc tttgagcata ttaatgtggt tggcttccga gaaaataact cgcttaggct
     1501 gctgacgagc cgtttcgtat atatagcgca atatcttatt atcacgcccc attagttctt
     1561 ttagttttgc atcatacgct tcccagttgt caatggtttt gcgagctacc cccgattcaa
     1621 tagcggcttt tgctacagca ggagctacaa tggttaacaa acgaggatct aagggtttgg
     1681 gtataatata ttctctacca aaactaattt ttttaagatt ataagccgca cttaccacat
     1741 caggcactga ccgttttgtt aattcggcta tcgctttcac ggcagcaact ttcatttcgt
     1801 catttatctt ggttgcacgt acatcgagtg ctcccctaaa aatataaggg aaacccaaca
     1861 cattattaat ttgattagga tgatccgatc gacccgtagc aaaaatgata tcttcacgcg
     1921 aagacatagc ttcttcgtag gatatttcag gatttggatt ggctaatgca aacacaatag
     1981 gattttggtt catcgtacga atcatatcag ccgaaagcac gccttctacg gataaaccta
     2041 aaaagacatc agctcctgct aaagcttctg ttaatgtttt aatcgttcta tccgttgcaa
     2101 atggcttttt tctatcattt aaatccgtac gttgtttgtt gataactcct tttgaatcaa
     2161 gcatcacaat attttcgagt tttgctccta actgaatata caatgatgca cacgagtttg
     2221 ctgctgctcc tgcaccatta accacaattt ttacgtcctc aatatttttg tttgttaatt
     2281 ccaatgcatt gattaaccca gcagcagaaa tgatggctgt accatgttga tcatcgtgca
     2341 taataggaat atccaaatca cgctttaatg catcttcaat ctcaaaacat tcgggagctt
     2401 taatatcttc taagttgata cctccgaagg taggtgcaat cgcttttact acttctataa
     2461 attttttagg gtctttttcg tttacctcaa tatcaaacac atcaataccc gcaaaaatct
     2521 taaacaacaa gcccttacct tccataacag gctttccagc tatggcacca atatcaccca
     2581 atccgagcac agctgtaccg tttgaaatta cagctactaa gttgccttta gcggtataat
     2641 catacgcttt actggcatct ttttctattt ctaaacaggg ttcagaaaca cctggggaat
     2701 aagctagtga taaatctcgt tgcgaactat agggtttagt tggtacaact tctattttgc
     2761 ctggtttacc ctctgcgtga taacgtaaag catcttcttt attaactttt gccataaata
     2821 ttatttttgc tacacagtag cgattaactt ataaattgta agaaaaaatt atacaaatgt
     2881 acgtaaatat ttattgatag agcattctac ttactacaat ccatctaaga agttactatt
     2941 tttaactctt tttacacatc atactaaggg cgttatttac tctgatactt tcatcacaat
     3001 ttttcctaca tgttgattcg actccattcg ttcatgtgcc tctttaatga gcgtccaatc
     3061 catcactgta tcaattatgg gttgcaaacg tccatctgaa aaagatggta aaaattgttc
     3121 agcaaactca cgtgctaatt ttctctgaaa tgataagggt cgattacgta atgtagagcc
     3181 aatgacagta attcgtttac caataatctt gcgtaaatca actcccgaaa cgtctgctcc
     3241 acccatcaat gctagcatta ccattctgcc gtccatagct aataccgaaa tattgtcgtt
     3301 aaaatatgaa gcccctaaaa aatcgactac cacgtcaatg ctatttttac ccattacttc
     3361 ttgtactaca tcaacccacg actctgtttt ataatcaatg cataccgttg ctcctaactt
     3421 agcacaaata gcatgcttac tggctgatgc agttacaact atttcggctt gcatggcatt
     3481 agccaattga atagctgcag tacctacacc tcctgatgcg gcgtgtagca aaactttctg
     3541 tcctcgttgc aactttccct gccactgtaa cgctaaaaat gcagtaagaa aagcttcggg
     3601 tatagctgcc gcttcttcaa aactcattgt atctggcatt ctccacagca tttcagcatc
     3661 aatagccaca taactagcat acccacctcc tggaatcaac ccaaacacct tatctccaat
     3721 tttccagggg ctcgaaacag gtgcttctgc aactattcct gctacttcta accccatcaa
     3781 agggctggct cctgcaggag aaggatattt cccttttcgt tgcaaaatat ccgccctatt
     3841 tactccaaat gcttttacct gcaccattac ttcattttct cttaacacag gtatatctac
     3901 ctctcctatg tacattgttt cagcatttcc tatgctattt tgtgtaattg ctttcattat
     3961 tttacttatt tgtattcgtt tatgcaaaga tacatatcta agctcagaat agtaccttgt
     4021 agatgcaaaa aaaagacctg caaactacat gcaggtcttc cttaaactat cagtatgtaa
     4081 tattatttaa tttcctgctg tggcgttttt atcagtttta ccaataaagc ccctagcaag
     4141 agcataatac ctgctaccat atacgaacct gcataagtac ctgttatatc agcaaccata
     4201 cctcccaata tcggacccac tatacctgca ataccccagc tagtgaagat aataccataa
     4261 ttaaccccta aattttttac gccaaagaaa tcggctgtta ctgcggggaa caatgcaaat
     4321 aaagccccgt aagctatacc tgccacggca aaaccaacaa ccaataatgg gaaagaagta
     4381 taaaatgaga aaacgaacat attaattgct tgtaataaga atacaattaa caaacctgtt
     4441 gtacgaccca ttttatcaga taaaaatcca cccaacacac gaccagaagt attaaatgca
     4501 gcaaaaagca ttactaacca aaaagcattt aactctggat ttcctgtttg cactctggct
     4561 atacttacaa tatgtccaat aagcatcaaa cctgctgtgg cactcaaaaa ataagtcgcc
     4621 cacaacaaat aaaatggctt tcttttcatc atggcttgcc aggtaaaatc agccgaatga
     4681 gctactacct tcgccccttc ttttgcctta ggtatataat tagcaggagg gttgtgtaaa
     4741 atagctccaa aaatgcttaa aaaaacgatg gcaataatac ccaaaatcat aaatgtacct
     4801 tgtacggtat ttccttgtaa caaataatta gttaacggag ccatgtagat agaagaaatt
     4861 ccaattcctg caacaacaat tcctgcaatt aaacctttct tgcgaggttc aaaccatttg
     4921 atagcagcag gtgttgctgc cgaataagca aaacccattc ccaaaccagc taccacacca
     4981 aatgtaagta ccatcatcca aggtgtatgt acaaatgcag acaagaaaat acccacacca
     5041 aataatattc caccacccaa agctacaaca cgtggaccaa acttatcttg taatttacct
     5101 ccaaaaacag tcataaaagc taataatgca acacatactg tataaggcaa agaggcttct
     5161 gtatttgtcc aaccttctgc tactaatgct gctttaatta cactccatga atacaataat
     5221 cctaaaatta aattaatccc catagcagag gatgttacca cccaagctgt cgctttttga
     5281 tttgaactca ttgatttttt gattttaatg ttttagttac tagtaattat ataaatagtt
     5341 cttcgaacac agaataaggc attttacaaa atcgatgcaa agaaaactat tttattttaa
     5401 ttagttagta ttttactgaa agatagatta ataaaaacaa cgcaaacgtt tgttttctta
     5461 caatcaaaca aaataaaaat gaattttagc ttaacaagat aaaaaggttt ttttattgca
     5521 atatttttca tacttttgat gtaaaattat agactattga cgtatgatga ttgacgaaaa
     5581 atatttattt gcttttgcat taactttatt tgcagggtta gcaactggca ttgggagctt
     5641 aatggctttt ttttctaaaa agtttaatcc aaatttttta tcagctgcct taggtttttc
     5701 agcaggagta atgatttatg tatcttttgt agagattttt ttcaaagcaa aagaatcgct
     5761 tacggcttca ttaggagatg ttactggcta ttggatgacg gttgtttcat tttttgcagg
     5821 aattgcctta attgcactca ttgataaact tgttccttct ggcgaaaacc ctcatgaaat
     5881 tagcaatatt gaagaagcta attacaaaaa tagtacgagt aatgacaaac tccttcgtat
     5941 gggtttattt tcagctttag ctattgccat acataatttt ccggaaggat tagcaacatt
     6001 tacggctgct ttaactgatc ccacattggg tataagtatt gcagttgcta ttgctataca
     6061 caatataccg gaaggagtag cagtttcagt tccaatttat tatgcaacaa aaaacaagaa
     6121 aaaagctttt tggatgtcat tgttatctgg attagccgaa ccagtaggtg caataatcgg
     6181 atttattatt ctacgtccat ttttagatgg tgctacattg ggaattatct ttggtgcagt
     6241 ggctggaatt atggtttata tttcgcttga tgaactactt cctaccgcac gtgagtatgg
     6301 aaaacaccat atcgctattg gtggtcttat tgcagggatg gctgttatgt cggttagttt
     6361 actattattt gcataaaatg ttcagaaatg aaattgcgct gtgttaggta tactttattt
     6421 ttattttttt tcttggtttt tacagcttgt ggctctttgg tagtgaagaa acaagaaaaa
     6481 atctctttgt acacacatac aacactaatt gatggtcaaa atcgatttta caaagtatat
     6541 gttcctagcc atataaagaa caatcagact ataccactag ttattgctct acacggcaat
     6601 ggaggtaatg ccgaggcact tattagagaa accaaatggc acacaaaggc ttcaagcgaa
     6661 gggtttttag tagttgctcc cgaagcttca cgacccaacc caaataaacc tcgcaatcag
     6721 agagataatc gtcaaacatg gaatgatggt tctggtcgat ttcattctgg agaacgctct
     6781 attgatgatg taaagtttat aaccacaatg atcaacgaat taaaaaacac atataatatt
     6841 gacaaaaaca agatttttgt aacaggtttt tctaatggtg catcaatgac tttccgcttg
     6901 ggaatggaat tggactgtat aaaagcaatt gccccggtag ctggagttaa ttggatcaaa
     6961 aataatacat ccaatagaaa aatttctctc ctgtatatta tcggagcaca agatagagct
     7021 aagccactag aagggggtat tacaaaaacg gcaaatggca ttgtgttaga aaaaacaccc
     7081 aagcccccta tatatgaaaa tattaaacga tgggctacat ttattgattg tattggggaa
     7141 ccaacaacat ttacccttgc ccaaggagtc agcggattgc gatttaccga ctgctcatct
     7201 aatacctcta ttgaatatat tattgttgat gatttggggc atatatggcc aggggcaaga
     7261 caaataatac caaaatcaat agtaggaaat gcttctgaga aactaaatgc cacggagtat
     7321 atttggaatt tttttaatac cgcaaaataa agaatatgaa tactgaaaat ataacattag
     7381 cattcaacaa gattataaca gatattgtaa attggctctt gtacgatttt acgtatatac
     7441 taattagttt agttgttgtc attgttttga tttggctact caatgccatc tctcgcaatc
     7501 tagaaaaagt tatcctaaag cgttcggatg ctttgcacga caaagaagct caaaaaagag
     7561 taaaaacttt aatgagcatt ggcagaggca ttatgcgcct cattttatgg gcaatttttg
     7621 cattaatttt actaaaacgt attggaatag aaataggtcc aattatcgct agtgcaggca
     7681 ttgcaggtat tgctatcggt tttggagctc aagaattaat tcgtgatttt ctagcaggtt
     7741 ttttcgtatt acttgacaat caaattagag cagacgacat cgtagtaatt aatggaacaa
     7801 ctggggtagt ggaaaaaatt gaattacgta caattcgttt acgcgaccat tctggtgtag
     7861 ttcatatttt tcaacatgga aaagtcaata ccttatctaa tatgaccaaa gaatggtcgg
     7921 cattagtatt tgaaatcggt gtagcataca aagaagatgt ggatacggta atagctatta
     7981 tgaaatcagt aggtgaagat ttagaaaaag attcaagatt ttcaggaaat atattagagc
     8041 ctatctctat acttggagta gaacaatttg ccaacagttc tattcaactc aaagtgcgta
     8101 tcaaaaccca aacaggggag caatggcaaa ctagaagaga atatatgcgt cgattaaaat
     8161 atgcatttga taaaaaaggt attgaaattc ctttccctca taccacactg tactggggcg
     8221 aaaaaagcaa acccatggat atttcactac aaaaataaat cgcaccactg gtggaactgc
     8281 acaacagaat gtgaactaaa tttttgcaat ttcgtttcaa tccttgtgat agataattct
     8341 tcttctagat gtgttttcga aaaaaataaa tcggcaaaac aaattaattg ctcttctaac
     8401 gatataggtt tcatgtctcg taatgggaga ggcaacttgc gagttacaat atcttccaaa
     8461 ctaatacctg tacctgtatg gcgttcgcaa actagggcat gctgaggcaa ttgttcttcc
     8521 atcaagatgc cagaccctaa atagccatga caaacatatg gataagaacc ataacaagca
     8581 acacttgggg cattggttaa atgtatccca atatcgtgca acatagctgc ctgaaaaaca
     8641 aattctgtat ctatcttcaa ttcaggatga ttgtgagcaa tagaaagggc tttattagca
     8701 acactctcgc tatgatttac caatatgtca tacgcttctt catttgaaga ataatatttt
     8761 tgtaaaatta catacggatt cattattgta ttggtttacc aaccatctca atggcttttt
     8821 tcacagtatt atcttgtaaa ttaagtattg gataaaatgc ttcatcacca agcaaattac
     8881 gagcaatata tgcttgtagt aaatttgcta agagagcttt cgatttcgca atctcttgac
     8941 tattgcggga aacacctttt tgttctgcat aacgaataaa ttcgctaaaa taagttgttt
     9001 tctttactaa gtattctact aatttttccc acgtgtcata agtagataat ttagctcgat
     9061 gcttatcaac aaaagcaaac gaaaaatcat atacaatgcc attatttaaa acgtttgtgt
     9121 aatatggtgt ataataggta gtatcatgag ggacaaaaat gtcgggcata atacctccat
     9181 tagcatatac agttcttcct ttggcagtat aatacttcaa actatcagca aaagaaatac
     9241 tatctttttg gaacatttca ccatgtaata ctcgtttttc tatttctaca tcataatttt
     9301 catccgactc gcctgacttg tacggtttct gaatagaacg acctgatggt gtgtgatagc
     9361 gagccacagt aagccttaat tcagagccat cggacaacgg aatttgttgt tgcactaaac
     9421 ccttaccaaa agagcgtctg ccaattaccc atcccctatc atgatcttga atggctccag
     9481 caaatatttc gctagcagaa gcagaccatt cgtttatcaa cacacagata ttctcttttc
     9541 ggaaattacc tcttccatct gcaatggcat cttctctggc ttgattaact ccctctgtat
     9601 atacaattaa ttgacctctt tttaaaaatt cattcgtcat ctgaatgaca gcggataaat
     9661 acccccctga attgttgcgt aaatcaacaa taatattatt gcattcttgt tcacgtaata
     9721 tgactaatgc atctaaaaat tcacggtatg tattagcccc aaaattacta attttgatat
     9781 agccagtaga atcggctatt acgtatacca catctatact ttttactggt acatctcctc
     9841 tcacaatatc aaaatcaaga ggcaaggggc ttccgttgcg tttaatccgt actttaacaa
     9901 tactgccctt ttctcctctc aatgttttta gcactgtggc attggtaaca tcagctccta
     9961 caaaactact atcattcact gcaataatac gatcacctgg caatattccc aatttttcag
    10021 aaggaccacc aggaatcaca ttcacaatca taaccgtatc attctgaata ttaaattgca
    10081 ccccaatacc actaaaacta ccttgtaact cagtagctga ttctgctact tcttctggag
    10141 ttaaataagc tgaatgaggg tcgagaaatt tcagtatctg tggtataaac tcttctgtta
    10201 aagcattttt atctataaca tccacataat tattgtctaa caaatacata atttcatcaa
    10261 acttattccc ttgaaccttt ttaggaatag caaaacctgt agtttgttct gcctgttgat
    10321 aatacaaatt tgctaacaat attccaaccg caatggatat accaataatg attggataaa
    10381 gcaatttatg tttttccata cgttaattat ctaaatcaat tttgagcact tctaccccgg
    10441 ctctcacgag caaatcaacc ccatcagtaa catggtattc attggcatac acaaccctca
    10501 caatacccga ttgaataatc aatttggcac attctatgca tggagatgtc gttacatata
    10561 acgtagctcc ttcgctacta ttgtgagaac gtgcaatttt tgtaatcgca tttgcctctg
    10621 catgaagcac atagggtttg gttttaaaac tttcgtcttc acaaatattt tcaaaaccac
    10681 acggcgtacc attgtaacca tcagaaataa tcattttatc ttttactaat attgctccta
    10741 cctgacggcg tttgcaatac gagttttcag cccaaataag agccatttgc atatatcttt
    10801 tgtctaatac taactgtttt gtattcatat ctcactatct aaaacgaatt gatactgttc
    10861 gctgttttat ttttccagta attgttgaat ggattgttcc aataaaggta acgaaacatt
    10921 gcgtgccact atatctcctt ccttatccaa caaaaataca gtgggtaagg ttgatatatt
    10981 atacgtttgc aacaaaggag atactaatcc tttcttatca tgaacaactg tatagggcaa
    11041 ctgtttggca acaacagaaa atacagcatc atcgtcaaac gaaacctgat aaatgcgtaa
    11101 tcctttgctg gcatatacac ggtaaagttc ttgtagcaat acagtatgtg catatgaatt
    11161 ttcggattga aacaacgaaa aatccactat agtggcacgc cctaaggatg ctgataaaga
    11221 acgctctacc ccttgcaccg tggacaagga aatatcaata aaacctttgc caactatttc
    11281 tctaactagt ttatcttctg catttttgcg tcgttgcata ccttttagca ccacgtcttc
    11341 gatattcttt gtacgtgccg tttgtggata atgcacttgc aagggtgtag ccactgcagc
    11401 atacactttt acatcttctt tatcataagg attaaacaca taatacccat taatctgctg
    11461 atacaaagca aaataagcca cacttgatcg cgtatcttga tatacctctt tgcgtaagaa
    11521 atcttttagt tgacgaacaa gctgcgtcgt ttcttctgta aaatcttgtg ccactatttt
    11581 gccacgttga tacttatctt gactagctgt catcttacgt tgtgtctgtg caacatagtg
    11641 cacaatggat tgcatgcgtt tagatgctgc tgaaccaact atactatctg cttgaccaaa
    11701 tgtttgtttg tttgaataga cagaaatcgt ttcggtagag tcggctacta aatacaatac
    11761 accttgttgc aaacgcaaac gcacatagtg aggttcaact gcacgaggaa aggaaaaaga
    11821 aaacttgcca ttttttttaa gtacttgata agcgactatc tctatttttc ccaactcaac
    11881 tttttccaaa aataaagtgt ctgtttcaac tcccgacact gtgccttgaa tggtatattc
    11941 ttccgtttga gagcatgatg acaataaaca aacaaacacc acgaataaaa ataagattga
    12001 ttgcttcata ctgctcatac tggtataaca ttacatgtac cgaataccct ataataattg
    12061 tgtaaataaa ccttgcaaaa atacaacatc tttcagctaa aatcaaaaaa atcgtatctt
    12121 tgtacgctta aaatgagtat tttattgtct attaacacat attgctaatg aataccgcat
    12181 tccgattaac acgtttttcc ctttttgttc ttattgtact ttctacaatg agttgtagaa
    12241 acgaacaaaa acaatttttt ataaacgaag gtttagtata tggtacctac taccgcacca
    12301 cctacgagca ctatcaagat atagaagttg acataaaaga agctatggcg aaagtaggaa
    12361 catctttatc tacatttgac agcctttctg taatttctcg gattaacaga aatgatagca
    12421 ccgtagtggc tgacacttat tttacggaag tatttacgac ggctcaacag gtttcaacac
    12481 atacgggagg tgcttttgac ataacagttg caccattggt aaacgcatgg ggatttggtt
    12541 ttgaccctca acatgaaaga acagccatcc taatagatag catcaaacaa tgggttggat
    12601 acaacacaat atccttacaa aacggcaaaa tcctgaaaca acacccacaa acgcaacttg
    12661 acgccagtgc tatagccaaa ggatatgcgt gcgatgtagt agctcaggta ttacgtgagg
    12721 ctggtatcga aaattttttg gtggatattg gtggcgaatt ggtagtaaaa ggacataata
    12781 agagaaacac tgattggcaa gtaggtatca acaaggcaat tgatgatgct acgcaacaaa
    12841 accaagaaat acaacaaatt atttcggtaa cagatgtagc cattgctact tcgggtaatt
    12901 atcgtcagtt ttactacgaa aacaacaaaa aaattgccca taccatcgac ccccgtactg
    12961 gctaccctac caatcattcg ctgttgagca ccaccgtaat tgctcctact tgcatgctgg
    13021 ctgatgcgta tgctactgct tttatggtaa tgggaaattg ggaagaaata atgaaagtag
    13081 taaacaatgc tgttcctcct attgctgttt tttgtatttt tgaagatact acaggcaaac
    13141 atatcgaaat ggcttctcct ggttttgaac agtacctgca ggaaaagtaa tcggtaattt
    13201 atcacaaacc aagcttactt acggataaaa tttgtgtatt ttatcaccac gtattttatg
    13261 tttaaacaaa aaaacaacta ttttttatcc ctctatgcca ttattaaaca aatccatcca
    13321 ataaaaattg cttcttctga tttcgaaaaa tatctctatc aaaagaagca ataaggtgtt
    13381 atcaggaaat tggaaaaatc ctacatattc ataccaccac agcagtgaat agtttgccca
    13441 gatacatagc tagacatatc cgaagccaag aaagtagcta cattcgccac atcttcgggt
    13501 gtaccgcctc tacgcaatgg aatttgtgca gcccattggt ctcttacctc atccgaaagg
    13561 gcagctgtca tttcggtaat aataaatcca ggtgcaatgg cattggctct aacacctcgc
    13621 gagcccaatt ctttggctat cgatttggtt aaaccaatca tacctgcttt ggatgcagaa
    13681 tagttacatt gtccagcatt accacttacg cctactactg acgacatgtt gatgattgaa
    13741 cctgtttttt gtttcatcat aataggtgtg atggcatgta caaagttgaa agctgatttc
    13801 aaatttacat tgattaccat atcccattgt tgctcagtca tacgcatcat caaaccatcg
    13861 cgggtgatac ctgcattatt tactaaaata tcaatacgtc caaattcttt taaaatttca
    13921 gataccacat tgtgtgtatc gtcaaaattg gcagcattag acgcatacgc tttggcttta
    13981 acaccataag cagctaattc tttttctacg gctttggcat tgtcatctac agctaaatct
    14041 gtgatggcta tatctgctcc ttctgaagcg tatttcaatg caatagcttt accaatacca
    14101 cgtgcagctc ctgttactac ggctactttt ccttttaata aactcataaa tgtatgtttt
    14161 ttattagatt taacggatgc aaatgtacgc attttatcag aaatcttata cattttatat
    14221 aaaaaagggg gaaatgtgat gttgcagatt actataaaca ataattttta cgctacatag
    14281 tattgttata aaacacaaag aggcaagatt tctctcgcct ctttatgcta aaaaaacaga
    14341 tagatgtttt ttagaatttt accacttttt tagtggctat tactttgtta gccttgtaca
    14401 tatgtacaat gtaaatacca cttgtccatt gagatgttgg tatggtaatg gcactatcaa
    14461 cattcgttgt atcatacatc gaaaccccta cagcattggt aactaatatt ttatctaatt
    14521 tttcagccga ctgtacatga agctgatctg taaacggact tggatacaaa actactgata
    14581 cagcctcgtt ttgtgttagt tgggttactt gatcaaaagt aactgtaata gttttatccg
    14641 ttgtcattgt tatagtttct gaagctgtag ttgataccaa atcgccactc catgccacaa
    14701 acacattccc atgattagga atggccgaaa tggttacttg ctcatccttg gctacaggca
    14761 cagcagtagt ataagctaca tcaccaactt ttactgaccc atttccaaca acttgtatgg
    14821 tcaaccattg ggtttcaact gggataattc cagaaaatgt aaccatataa tcattgttgg
    14881 taaaatgagt aattgtatta tctacattaa ttagtcgtgg ttgtaaaagt ggaaatcctg
    14941 tcttttcaat caatatggca taatccccag gaggaactcc attaaaattg taatacccat
    15001 tttcatctgt cagagtctga gagataatct gattgttggc aacatcaaac aaatatacaa
    15061 gtaattgttc aagaggatta ttgaaggctc tcttatttta tggcacttac taccaacggg
    15121 tcatttgccc tactaccacc gactcatttt taaactcgtt gtgcgtataa aaagaattat
    15181 gaggcgaaaa acaaaacaaa ataacaggaa agcctaaggg tggaactaag atggagtcgt
    15241 ttgaatcaaa agaaacatac gaaaaactat caatgtaggg taacaatgag tctgtaacta
    15301 cagggaaaaa tgttgcatgt tgcattcaat tattttttta caaaaataga aaaatttatt
    15361 taactttgta aaaaaataaa tatggcaatt atcaacttag aaaatatgga gttttggtct
    15421 tatcatggtt gttttgaaga agaacgccgc ataggcacca aatacgtaat aagtgtatcg
    15481 ttagcggtag atatgcaaca agcaatggca tcagacaact tacacgacac gattaactac
    15541 caagaagtgt attcgataat aaaagaagaa atggacatcc catcaaacct tatcgaacac
    15601 gtagcgggca gaatacaaca cagactaaaa gaacgtttcc cacaaagcac tcaacaaaca
    15661 atttctttat ccaaattcaa tcctccctta ggcggtcaaa ttgaaaaagt taccattgtt
    15721 tt
//
LOCUS       DAOSRP010000032        15135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4027_length_15135_cov_5.140318, whole genome shotgun sequence.
ACCESSION   DAOSRP010000032 DAOSRP010000000
VERSION     DAOSRP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_15135_cov_5.140318"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLJ82_03290"
     CDS             <1..749
                     /locus_tag="PLJ82_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOR40565.1"
                     /translation="VKVKLTLQEVAKGVEKKIKVKKYVTCSKCGGSGAEDASSIVTCS
                     SCKGTGSVTRVVNSMFGQMQTSSACPTCQGEGKIITKKCSKCNGEGVEIAEEVISINI
                     PAGVQEGMQLSMSGKGNAARRGGVNGDLLILIEEEPHPELIRDDNDLIYNLMLSLPTA
                     ILGGTVEIPTIEGKAKIKIESGTQSGKILRLKGKGLPSVNSYGTGDLLVNIGVYIPES
                     LTKEEKKIFEGFEKSQSFSPSKSSKKNFFS"
     gene            754..1827
                     /gene="mnmA"
                     /locus_tag="PLJ82_03295"
     CDS             754..1827
                     /gene="mnmA"
                     /locus_tag="PLJ82_03295"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR40566.1"
                     /translation="MNIAALVSGGVDSSVVVHRLKEMGYQPTIFYIQIGMEDETGYMG
                     CTAEEDIEIVSFIAKKYGLSMEVVSLQKEYWDQVVDYTIETVKKGLTPNPDIMCNKLI
                     KFGSFNQKYGKDFDKIATGHYAQTLIKDDTYYLSTAVDKHKDQTYFLSRISYEQLSKS
                     LFPIGDLPKSRVREIASLQQLPSATRKDSQGICFLGKINYSDFIKRYVGVKKGHIIEY
                     ETGKVVGQHNGVWFHTIGQRKGLGLSGGPWYVVAKQVDENIVYVSNGYDVVTQYGMMI
                     PLQDLHIISGNPTIYDNPTRVAFKIRHTPEFTFGTIHRKGNTYCIESEEKIQGIAPGQ
                     FGALYDEKCEICVGSGIIAEYKL"
     gene            complement(1901..1974)
                     /locus_tag="PLJ82_03300"
     tRNA            complement(1901..1974)
                     /locus_tag="PLJ82_03300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:tct)
     gene            complement(2041..4353)
                     /locus_tag="PLJ82_03305"
     CDS             complement(2041..4353)
                     /locus_tag="PLJ82_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOR40567.1"
                     /translation="MSRVIRYISLLIIVLLSACSATKYVPENKYLLDNVRIKSDIKNF
                     KSTELKTFLRQTPNSKMFDLISFNLGIYNLSGNNTDVWINRFLKRIGNPPIIFDSTLT
                     ERSEVELQRFFKNKGFENAQVESSVVFNKRKAKVTYNIISNNPYVINTIEYRIQDPII
                     KDLIEKDSSSSLIRKGNLFDVDVLDNERVRITRLLRNNGYYYFNKEYVRFLADSSLNT
                     NSVDVIIRIRKMERTGERNTVLRSNHEVFSINSITYILVNDVLDINGYTNQKTKKDTL
                     FYKDKRIIYNNLFINPSILDDNTFITIGDNYSEYDTEKTYRRINNLHVSQFLRIDFSE
                     TPLLSDSIRKLDCTILLTPSNNQSFAIEAEGTNSAGDFGIAGKFGYTHRNAFTGGEKF
                     GLQLRASNEAIGSIRNILSYSAWEIGGETTLQFPRLMFPFVDRDFQRNSNANTEFNLN
                     YSYQTRPDFARTIAGIGLKYRWDSYRQSNMFHTLDFLDLSYVYLPPYSISDEFRNKYL
                     NSSTLLKYSYEDHLILRTGYSFSYTSKLLQQRRNGFSAKAGIETAGNSFYGIASLLKT
                     PKQENSYVIGNIKFSQYIKLDGEYSYTQFLDPSNRIVYHIAMGIGIPYGNSSILPFEK
                     RYYGGGANSVRGWQVRTLGPGSYNNNGITDYMSQSGDMRADFNIEYRTKLVWLLELGA
                     FIDAGNVWTLLPYGEQVGGNFDINNIYREIALGYGIGIRFDFSYFLLRLDWGIKAYDP
                     SKIGSDAWRFSSNWNIMKDTALHFAVGYPF"
     gene            4447..5193
                     /locus_tag="PLJ82_03310"
     CDS             4447..5193
                     /locus_tag="PLJ82_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR40568.1"
                     /translation="MLSKSKIKFIRSLRSKKQRNDLRLFIAEGQKTVDDLLLYFPCSM
                     LFHTSEYCTSHSVSYLQEVTPEELKQISFLEEPQQVLGVFVMPEPIWSNDYLEGELII
                     ALDMIQDPGNLGTILRVADWFGIKHIVCARGTVDVFNPKTIQATMGAIGRVHVYIVDM
                     GEFLQQLPHKIPVYGTFLEGENIYSTNVQNEAVIIMGNEGNGISQAIEKHVTHRITIP
                     SFPPNQSTSESLNVAMATAIVCAEFRRRLF"
     gene            complement(5174..5539)
                     /locus_tag="PLJ82_03315"
     CDS             complement(5174..5539)
                     /locus_tag="PLJ82_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40569.1"
                     /translation="MKRIAAHYIVVSDKRILKKHMIVLNVSLCVENILPLNHETPSTQ
                     FYNGIIFSLQNTPKDSIEDIIQWQTNNSGKTIYDYLSNMGAQPIKIGTHLSELYVLLD
                     IDFVSSIVTAHSSIRKVVS"
     gene            complement(5550..6317)
                     /locus_tag="PLJ82_03320"
     CDS             complement(5550..6317)
                     /locus_tag="PLJ82_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOR40570.1"
                     /translation="MKSLIFVLSLFFVTSTFLSCTKEERVPSSVISEEKMVDILVDVH
                     MVEAMARHNQLKDNNQRANMYYNYILEKHNITLNQLDSSISWYAKHPTTYIRVYDQII
                     PRLEHSLEMVRTNANTGGQALRTLDIWEKKNEYQLTSLQNSNVPFRIEENSTIRFSLG
                     DEFVLKTKFSCKNVPSSLQLVMQVKIHFTDSTESIFEKKIVTTNPDFEQILRIQPDSK
                     KQIAMISGDLCYLIDKIDNPIPIEIRIFNVEMEQITK"
     gene            complement(6330..6953)
                     /locus_tag="PLJ82_03325"
     CDS             complement(6330..6953)
                     /locus_tag="PLJ82_03325"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOR40571.1"
                     /translation="MKKNKLTISIISITIILTILLLDQIVKIWIKTHFAIGDEIKITN
                     FFYLHFIENEGMAFGMSFFDKKYLTLFRIVASIGIAYYIYKSIKNNSEQLFIISLSLI
                     FAGAIGNIIDSLFYGQIFTESTLFSNATWTPWGQGYAPIFYGKVVDMLYFPLISGTYW
                     SWIPIVGGTSFTFFAPVFNIADASITIGVILILVYEIFFSPSSKKIQ"
     gene            complement(7140..7520)
                     /locus_tag="PLJ82_03330"
     CDS             complement(7140..7520)
                     /locus_tag="PLJ82_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOR40572.1"
                     /translation="MGEKTRYSDSELEEFRAIIKDKLEKAFVDYEQLKAALNNLDGND
                     TTDTSPTFKVLEEGATTMSKEEAGRLAQRQMKFIQHLQAALVRIENKTYGICRETGAL
                     IPKERLRAVPHATLSIEAKQSGKK"
     gene            complement(7614..11045)
                     /gene="ileS"
                     /locus_tag="PLJ82_03335"
     CDS             complement(7614..11045)
                     /gene="ileS"
                     /locus_tag="PLJ82_03335"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR40573.1"
                     /translation="MNTKFSEYTHLDLSAINKDILAYWDKHDIFKKSIDTRTGNPTFV
                     FYEGPPSANGMPGIHHVMARTIKDLFLRYKTMKGYQVQRKAGWDTHGLPVELGVEKSL
                     GITKEDIGKSISVEEYNAACRKDVMKYTKEWESLTHKMGYWVDMNHPYITYDNKFIES
                     IWWLLKELYNKNLLYKGYTIQPYSPAAGSGLSSHELNQPGCYKDVKDTTVVAQFEIDN
                     PIAELTQFGKAYFLAWTTTPWTLPSNTALCVGATITYVAVQTYNPYTFEPQTVILAKD
                     LVSAHFNGKASSLSLSEYTAGDKLIPYSIIAEYKGSELVGMTYKQLIPFFKPDEGAFS
                     VISGDFVTTEDGTGIVHIAPTFGADDARVAKMNNIPAMLLIDKNGNERPLVDLQGKFY
                     TINDIDNDFVAQRTNIALYSPFAGRYVKNAYDTSLNENDATLDIDLCVMLKQQGLAFK
                     IEKHIHNYPHCWRTDKPVLYYPLDSWFIRSTACRETMVELNNTINWKPQATGTGRFGK
                     WLENLQDWNLSRSRYWGTPLPIWRTEDKQEEICIGSIAELMAEIEKAIQAGMMNKNPF
                     KDFVVNDFSEDNYGISRIDLHRPYVDNIILVSPSGKPMKRETDLIDVWFDSGAMPYAQ
                     LHYPFENKELIDKQTNFPADFISEGVDQTRGWFFTLHAISTMIKGSVAFKNVVSNGLV
                     LDKNGNKMSKRLGNAVDPFSTIETYGSDALRWYMMTNASPWDNLKFDTEGIEEVKRKL
                     FGTLYNTYSFFVLYANVDGFAYKEPEVPMEQRQEIDRWIISLLHSLVKEVDENYADYE
                     PTRAGRAITTFVTDNLSNWYVRLNRRRYWAGEMTLDKLAAYQTLYTCLETVAKLMAPI
                     APFFAERLFLDLNSVTQKDISESIHLAQFPTFSESLINKQLEERMFIAQTITSLVLAL
                     RKKVNLKVRQPLSSIMIPVLDNNMKEDIEAVKELILNEVNVKSIQFVDNSAGILVKRI
                     KADFKKLGPKCGKHMKQVSTLLQEMSQPQIAEFEKIGNYTLSVENNVNITISTQDVEI
                     ISEDIPGWLVANEGKITIALDITLTDELKEEGIARELVNRIQNIRKANGFDITDRISI
                     VIEKNPLFNQAIENYKQYIASQTLAKSIEISDKINNGIELEFENDKLYLLIQKTA"
     gene            complement(11168..11629)
                     /locus_tag="PLJ82_03340"
     CDS             complement(11168..11629)
                     /locus_tag="PLJ82_03340"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOR40574.1"
                     /translation="MIQRIQSVYLLIISILGVCLWFFPIASITHDIHVYNITTWGIQL
                     TESNQTIYQFIPYLFLVLLLPIISFSSIFIYKKRILQMRLNVIAMILMIFLYGVIYFY
                     IDFATTIISKNSIIDYKLPIIIPVINLILCYLAIRAIGKDEALIRSLSRIR"
     gene            complement(11718..11906)
                     /locus_tag="PLJ82_03345"
     CDS             complement(11718..11906)
                     /locus_tag="PLJ82_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40575.1"
                     /translation="MKKLVLFVATIVAVAFASCAEKATEEAVVEEVVEEVVEVVDSAA
                     VVVDSAVVVVDSAAVVAE"
     gene            complement(12086..13753)
                     /locus_tag="PLJ82_03350"
     CDS             complement(12086..13753)
                     /locus_tag="PLJ82_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR40576.1"
                     /translation="MIKENFIKLFETSFKENWELPAYTDYTLGTSMTYGEVALKIEKL
                     HLLFAESKIKPGDKIALIGKNTPHWAITFVATVTYGAVIVPILQDFNPNDVHHIVNHS
                     ETVFLFCSDSIWDNLEEDKMENIQAAFSIDDFRCIHQKDGAGIQKIVGNLTSKFEKKY
                     PQGFTQSHIQYTSRDNSELAMLNYTSGTTGFSKGVMLTANNLAGNITFGFKTQLLKKG
                     DRVLSFLPLAHAYGCAFDFLVPTCSGSHIYFLNKIPSPKVLLKAFEEIKPNVIFMVPL
                     ILEKVYKKSIQPSLNKTGIKLALNIPLLNKKIYEQVNKKLTESFGGEFTEIVVGGAAL
                     NKEVEEFLTKIGFRFTIGYGMTECAPLISYASHTEFRPSSAGKILDTMQVKIDSPDPY
                     NIVGEILVKGEHVMQGYYKNELATSEAFTDDGWLKTGDLGTIDEHGYIFIKGRSKSMI
                     LGANGQNIYPEEIEAKLNNLPFIMESLVIEKHGKLIALVYPDYEAMDGSQLTQQDLEI
                     IMEENKNNLNNVVGTYENVSKIHLYPNEFEKTPKRSIKRYLYTNISI"
     gene            complement(13850..14629)
                     /gene="mazG"
                     /locus_tag="PLJ82_03355"
     CDS             complement(13850..14629)
                     /gene="mazG"
                     /locus_tag="PLJ82_03355"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOR40577.1"
                     /translation="MKKKLEAVENVIHILDELRVKCPWDKKQTNETLRVLTIEETYEL
                     SDAILQGNNQQIKKELGDLLLHILFYAKIGEEKSAFTLKDICDALAEKLIYRHPHIFG
                     TEQAQTSQEVEKNWEKLKLKEKEGNKTILSGVPSSLPSIIKAHRIQDKARSAGFDWEK
                     KEDVWKKVQEEISELTVEIDSMNKEKMEKEFGDVFFSLINAARLYKINPDNALEHTNK
                     KFIQRFTYLEEYAKQHEIALSDMPLAKMEEIWQLAKKEENM"
BASE COUNT         4898 a         2392 c         2462 g         5383 t
ORIGIN      
        1 gtgttaaagt aaaattaaca cttcaagaag tagccaaagg tgttgaaaaa aagattaagg
       61 taaaaaaata cgttacatgt tctaaatgtg gtgggagtgg agcagaggat gcttcttcta
      121 ttgttacatg ttcatcatgc aaaggcacag gaagtgtaac aagggttgta aactcaatgt
      181 ttggacaaat gcaaacgagt tccgcgtgtc ctacttgcca aggtgaaggt aaaattatta
      241 ccaagaaatg ctccaaatgt aatggtgaag gagtagaaat tgctgaagaa gtaatttcta
      301 tcaatattcc agcaggagtg caagaaggta tgcagctatc tatgtcagga aaaggaaatg
      361 ctgcacgcag aggtggggta aatggtgatt tattgatttt aatagaagaa gaacctcatc
      421 ctgaattaat acgtgatgat aatgatttaa tttataattt aatgttatca ttgccaactg
      481 ctattttggg tgggactgta gaaataccta ctattgaagg gaaagcaaaa attaaaattg
      541 agagtggaac acaatcgggt aaaatattac gtttgaaagg caaaggtttg cctagcgtaa
      601 attcttatgg aacaggagat ttattagtga atataggggt gtatatacca gaatctttaa
      661 caaaggaaga aaagaaaatc ttcgaaggat ttgaaaaatc tcagtcattt tctccttcta
      721 aatcatcgaa aaagaatttt ttcagttaag taaatgaata tagcagcctt agtttcagga
      781 ggagtagata gctcagtagt agtacatcgt ttaaaagaaa tgggctatca gcctactatc
      841 ttttacattc aaataggaat ggaggatgaa acaggctata tgggttgtac ggcagaagaa
      901 gatattgaga tagtatcatt tattgccaaa aaatatggat tatcgatgga ggttgtttct
      961 ttgcaaaaag aatattggga tcaggtagta gattatacca tcgaaacagt aaaaaaaggc
     1021 ttgacaccaa atccagatat aatgtgcaat aaattgatta agtttggaag ttttaatcaa
     1081 aaatatggca aagattttga caaaatagca actggtcatt atgcccaaac tttaattaaa
     1141 gatgatacat attatttgag tacagctgtt gataaacata aagatcaaac gtatttttta
     1201 tctcgtattt catacgagca actttctaaa tctctttttc ctattggtga tttgccaaaa
     1261 tctcgtgtta gagagatagc ttctcttcaa caattaccta gtgctacccg aaaggatagc
     1321 caagggattt gttttttagg gaaaatcaat tattcagatt ttataaaacg atatgtagga
     1381 gttaaaaaag ggcatattat tgaatacgaa acgggaaaag tagttggtca gcataatggc
     1441 gtttggtttc atactattgg acaacgaaaa ggattgggat taagtggagg tccatggtat
     1501 gtggtagcaa aacaggtgga tgaaaatatt gtgtatgttt ctaatggata tgatgttgtt
     1561 acacagtatg gtatgatgat tccacttcaa gatttacata ttatatcagg taatccaact
     1621 atatacgaca atcccactcg cgtagccttt aaaattcgtc atactcccga gtttactttt
     1681 ggtacaatac atagaaaggg aaatacttat tgtattgaat ctgaagaaaa aattcagggt
     1741 attgctccag gtcagtttgg tgcattatac gatgagaaat gtgaaatatg cgttggaagt
     1801 ggtatcattg cagagtataa actctgattg tgattaatat ataggtatat ataccaaaaa
     1861 aacgaggtaa agcagttagt ttctttatct cgtttttttg tgattccgaa gcgattcgaa
     1921 cgcttgaccc acgccttaga agggcgttgc tctatccagc tgagctacgg aacctcgttt
     1981 tgtgggcaca aaggtagaaa aaattttttt ctttgccaat tttttatagt tttaatttta
     2041 ttagaaagga tagcctacag caaaatggag tgctgtatct ttcataatgt tccaattact
     2101 cgaaaaccgc caagcatcag aacctatttt tgatgggtca tatgctttaa tcccccagtc
     2161 taaacgtaat aaaaaataag agaaatcgaa tcgaattcct attccataac ctagtgcaat
     2221 ttctctatat atattattga tatcaaaatt gccacccact tgttctccat atggtaaaag
     2281 agtccacaca ttacctgcat caataaatgc tcctaactct aacaaccata ctagttttgt
     2341 tctatattca atattaaaat cagcacgcat atcgccagat tgactcatat aatctgtaat
     2401 tccattattg ttataagaac caggtcctaa tgtacgtact tgccatcctc ttacgctatt
     2461 agcacccccg ccatagtatc ttttttcaaa aggtaaaatg gaactatttc cataaggaat
     2521 tccaataccc attgcaatat gatatactat tcgattgcta ggatcaagaa actgagtata
     2581 actatattct ccatctagtt ttatgtattg cgaaaattta atgttcccta ttacataact
     2641 attttcttgt tttggtgttt tgagtaaaga tgctattcca taaaaactat ttccggcagt
     2701 ttcaatacct gcttttgcag aaaaaccgtt tcgtctttgt tgtaataatt tacttgtata
     2761 tgaaaaagaa taccctgtac gtaagattaa gtggtcttca taactatatt tcaataaagt
     2821 agagctattt agatatttat ttctaaattc atcagaaata gaatagggtg gtaaatatac
     2881 atagcttaaa tcaagaaagt ctagtgtatg aaacatattt gattggcggt agctatccca
     2941 tctgtatttt aacccaattc cagcaatggt tcgagcgaag tcaggtcggg tttgatagct
     3001 ataattgaga ttaaattcag tattagcgtt actattacgc tgaaaatctc tatctacaaa
     3061 aggaaacata agtcgaggaa attgcaaggt agtttcacca ccaatttccc aagcactata
     3121 tgataatata ttacgaatgc taccaatagc ttcgtttgat gctcttaatt gtaaaccaaa
     3181 cttttctccc cctgtaaatg cattacgatg tgtataacca aactttccag caataccaaa
     3241 atcaccagca gaatttgtcc cttctgcttc aatagcaaat gattgattgt tactgggtgt
     3301 taataaaatt gtacaatcta acttgcggat actatcagag agtaaagggg tttcgctaaa
     3361 atcaatgcgt aagaattgag acacatgtag attattgatt cttcgatagg ttttttcggt
     3421 atcatattct gaataattat ccccaattgt aataaacgta ttatcatcta atattgatgg
     3481 gttgataaat aaattgttgt aaattattcg tttatcttta taaaaaaggg tgtctttttt
     3541 tgttttttga ttggtatatc cgttaatgtc caatacatca tttactaaaa tatatgtgat
     3601 gctattaatg gaaaaaacct catgattgct acgcaataca gtgtttcttt ctcctgtacg
     3661 ttccattttt cgtatacgta taataacatc tacagaatta gtattaagcg aactatccgc
     3721 taaaaatcgc acatattctt tattaaaata gtagtaccca ttattgcgta ataatctagt
     3781 aattctaaca cgttcgttat ctaacacatc cacatcaaat agatttcctt tgcgtataag
     3841 agaagaacta gaatcttttt ctatcaagtc ttttataata ggatcttgta tacgatattc
     3901 tatggtattt attacatatg gattattact aataatgttg taagttactt ttgctttacg
     3961 tttattaaaa acgacggaag attcaacttg tgcattttca aagcctttgt ttttaaaaaa
     4021 tcgttgtaat tcaacttctg aacgttctgt taacgttgaa tcaaaaataa tggggggatt
     4081 tccaattcgc tttaaaaaac gattaatcca tacatcagta ttgttgcctg ataaattata
     4141 aatacctaaa ttaaaactaa ttaaatcaaa catttttgaa ttaggtgtct gacgtaagaa
     4201 tgttttcagt tccgttgatt taaaattttt tatatcagat ttaatacgta cattgtcaag
     4261 taaatacttg ttctctggta cgtacttggt agcactacat gcacttagta atacgataat
     4321 tagcaggctt atataccgaa tgacacgact cataacttaa ttttgcacaa atttaccaca
     4381 aaattaaaat aataacaatt acttttgcgt aatagacgat aaaaataata attattctca
     4441 atatttatgt taagtaaaag taaaataaag tttatacgtt ctttacgttc taaaaagcaa
     4501 cgaaatgacc tacgattatt tattgctgaa gggcaaaaaa cagtagatga tttattgtta
     4561 tactttccat gttctatgtt gtttcatacg agcgagtatt gcactagtca tagcgtttct
     4621 tatctacaag aagtaacacc tgaagaatta aaacaaataa gttttttaga ggaacctcaa
     4681 caggtgttag gggtttttgt aatgcctgaa ccaatatggt ctaatgatta tttagaagga
     4741 gaattgataa tagcgttaga tatgatacaa gatcctggta atttaggtac tatacttcgt
     4801 gtagcagatt ggtttggtat taagcatatt gtgtgtgctc gtggtacagt ggatgtcttt
     4861 aatcctaaaa cgatacaagc aactatgggt gcgattggta gggtacatgt atatattgta
     4921 gatatgggtg agtttttgca acaattacct cacaaaattc ctgtttatgg aacttttctt
     4981 gaaggagaaa atatttactc tacaaatgtg cagaacgaag ctgttataat tatgggaaat
     5041 gaagggaatg gtatatcaca agcaatagaa aaacatgtta ctcatagaat cactattcca
     5101 tcttttcctc ccaatcaaag tacttctgaa tcgcttaatg tggctatggc tactgcaata
     5161 gtttgtgcag aatttaggag acgacttttc taatagaaga gtgtgccgta acaatagatg
     5221 aaacaaaatc tatatcaaga agaacatata attcagatag gtgagtgcca attttaatag
     5281 gttgagcccc catgttactt aaataatcgt atattgtttt tccactattg tttgtttgcc
     5341 attgtattat atcctctatt gaatccttcg gtgtgttctg taaggaaaaa ataattccat
     5401 tataaaattg agtagaagga gtttcgtgat ttaatggaag aatgttttct acacataagg
     5461 aaacgtttaa aacaatcatg tgttttttta atattctttt atcagaaaca actatataat
     5521 gggctgctat tcgtttcatg cacacattgc tatttcgtaa tttgctccat ttctacatta
     5581 aaaatgcgaa tctctattgg aattggatta tctattttat ctatcaaata acataaatct
     5641 ccactgatca ttgctatttg tttttttgag tcgggttgaa ttcttaaaat ttgctcaaag
     5701 tcggggtttg tagttacaat ttttttttca aaaatcgatt ctgtagagtc ggtaaagtga
     5761 attttaactt gcattactag ttgtaaagaa gatggtacgt ttttacacga aaattttgtt
     5821 tttaaaacaa attcatctcc aagtgaaaaa cgaatagtag agttttcttc aattcgaaaa
     5881 ggaacattgc tattttgtag ggatgtaagt tgatattcat tttttttctc ccagatatct
     5941 aaggttctta aagcttgtcc acccgtattt gcatttgttc taaccatttc caacgagtgt
     6001 tcgagtcttg gaatgatttg atcatatact ctaatatagg tagtgggatg tttggcatac
     6061 caagaaatag agctatcaag ctgattaagt gtaatattgt gtttttcaag aatataattg
     6121 tagtacatat ttgcacgttg attgttgtct tttaattggt tatgacgagc catcgcttct
     6181 accatatgta catcaaccaa tatatcaacc attttttctt cagaaataac agaagaagga
     6241 actctttctt cttttgtaca agaaagaaat gtactagtta caaaaaatag agaaagaacg
     6301 aaaataagtg atttcatact attatgtttt tattgaatct ttttggatga tgggctaaaa
     6361 aagatttcat aaacaagaat taatatgacg ccaatagtaa ttgaggcatc tgcaatatta
     6421 aatactggtg caaagaaagt aaacgatgtt cctccaacta taggtatcca agaccagtaa
     6481 gtaccactaa ttaggggaaa atatagcata tctacaactt ttccataaaa aataggggca
     6541 tatccttgtc cccaaggtgt ccaagtggca ttagaaaata acgtactttc tgtaaaaatt
     6601 tgtccataaa ataaagaatc aataatattc ccaatagcac ctgcgaatat tagagatagt
     6661 gatattataa atagttgttc gctattgttt tttatcgatt tgtatatgta atatgcgatc
     6721 ccaattgatg ctactattct aaatagggtt aaatattttt tatcaaaaaa agacattcca
     6781 aatgccatgc cttcgttttc aataaaatgc aaataaaaga aattagtaat ttttatttca
     6841 tcgccaatag caaaatgtgt ttttatccaa atttttacta tctgatctaa aagcagtatt
     6901 gtcaaaatga ttgtaatgct tatgatggat atagtaagct tatttttttt catcgtttat
     6961 tcgtcttaat tatagacaat atcgttgttt atatttcgtt tattccacat tagtttgcaa
     7021 aggtaatcat tattcagttt aaattacaga acttaggaat aactttaact ctaaaaaaga
     7081 tatcgacaag accataaagt acagccctgt cgataatttt tgtaatataa ctaatttgtt
     7141 tactttttcc ctgattgttt tgcttcaata ctaagtgttg catgaggaac tgcacgtaat
     7201 ctttcctttg gaattaacgc tccagtttct ctacaaatac catatgtttt gttttcaatt
     7261 cgcactaatg ctgcttgtaa atgctgtata aatttcattt ggcgttgtgc taatctgcca
     7321 gcttcttctt tagacatagt agtagcacct tcttctaata ctttaaaagt aggagaggtg
     7381 tcagtggtgt cattaccatc caaattgttt aaagcagctt tcaattgttc gtaatcaaca
     7441 aatgcttttt ctaatttatc ttttataata gctcggaatt cttccaattc gctatctgag
     7501 tatctagtct tttcgcccat agttgtatgt attagtattt aatataaagt ttactgcgaa
     7561 tataactcct tgttttcaca tttcaaaata tattcctcaa aaaaatatca atatcaagca
     7621 gttttttgaa ttaataggta tagtttgtca ttttcaaact ccaactcaat tccattgttt
     7681 attttatccg aaatttctat gcttttagct aatgtttgag aagctatata ttgtttataa
     7741 ttctcaattg cttggttaaa gagaggattt ttttcgatta caatcgaaat tctatctgta
     7801 atgtcaaacc catttgcttt acgaatattt tgtatacggt ttaccaattc tcttgcaatt
     7861 ccttcttctt ttaattcgtc agttaaagtt atatctaatg caatggttat tttaccttcg
     7921 tttgcaacca accagcctgg tatgtcttct gatataattt cgacatcctg tgtggatata
     7981 gttatattta cgttgttttc aacagacaaa gtataatttc ctatcttttc gaattcagca
     8041 atttgtggtt gagacatttc ttgtagtagg gtggatactt gtttcatgtg tttaccacat
     8101 tttggaccta attttttgaa atcggctttt atccgtttca ccaatattcc cgctgaattg
     8161 tctacgaatt gaattgattt tacatttact tcgtttaaaa tcaattcctt aactgcttct
     8221 atgtcttctt tcatgttatt gtccaatact ggaatcataa tagaggaaag aggttgacgt
     8281 acttttaaat ttactttttt tcttagagct aatactaaag acgtgatagt ttgggcaata
     8341 aacatgcgtt cttcaagctg tttatttatc aacgattctg aaaaagtagg aaattgggct
     8401 aagtgtattg attctgaaat atctttttgt gttacactat ttaaatcaag aaataagcgt
     8461 tctgcaaaaa atggagctat aggagccata agttttgcca ctgtttccaa acaggtatac
     8521 aatgtttggt aagcagctag tttatctagc gtcatttctc ctgcccagta gcgtctgcgg
     8581 tttaatcgca cataccaatt gcttagatta tctgtaacaa atgtagtaat ggctcttcca
     8641 gcacgtgttg gttcgtaatc agcataattt tcatctactt ctttaactaa tgaatgaagc
     8701 aaagagatta tccatctgtc aatttcttga cgttgttcca ttggaacctc tggttctttg
     8761 tatgcaaaac catctacatt agcgtataaa acaaaaaaag aatatgtatt gtataaggtg
     8821 ccaaatagtt tgcgttttac ctcttcaatg ccttcagtat caaattttaa gttatcccaa
     8881 ggagatgcat tggtcatcat ataccatcgt agggcatctg aaccataagt ttcaatagtg
     8941 ctaaatgggt ctactgcatt acccaaacgc ttagacattt tattgccatt tttatctaat
     9001 actaagccgt ttgaaactac atttttaaac gccaccgagc ctttaatcat cgtagaaata
     9061 gcatgtagag taaagaacca tccacgtgtt tgatctacac cttctgaaat aaaatcagca
     9121 ggaaaattag tctgtttatc aattaattct ttattttcga aaggatagtg tagttgagca
     9181 taaggcattg ctccagaatc aaaccataca tcaattaggt cggtttctct tttcattggt
     9241 tttcctgatg gagatactaa tatgatgtta tctacataag gtctatgtag gtcaatacga
     9301 gatattccat aattatcttc agaaaaatca ttgacaacaa aatctttaaa aggattttta
     9361 ttcatcatcc ccgcttgaat ggctttttca atttcagcca ttaattcggc aattgaacca
     9421 atacaaattt cttcttgttt atcctctgtc cgccatattg gtaggggtgt tccccaatac
     9481 ctactacgag ataaattcca atcctgaaga ttttcgagcc attttccaaa acgtcctgta
     9541 ccagtagctt gtggtttcca atttattgtg ttatttaatt caaccattgt ttcgcgacaa
     9601 gctgtagagc gtataaacca gctatctagt ggataataca atacaggttt gtctgtacgc
     9661 caacaatgtg gatagttatg tatatgtttt tcaattttaa aagctagtcc ttgttgtttg
     9721 agcattacgc acaaatcgat atccaacgtt gcatcattct catttagcga ggtgtcgtag
     9781 gcatttttta catatcttcc agcaaaaggg ctatataggg ctatatttgt tctttgagct
     9841 acaaaatcat tatctatatc gttaatagta taaaacttcc cctgaaggtc aactaagggg
     9901 cgttcattac catttttatc tatcaataac atagcaggaa tgttgttcat ttttgctaca
     9961 cgggcatcat cagcaccaaa agtgggtgca atatgtacta tacccgtacc atcttcggtt
    10021 gttacaaaat ctccactaat aacgctaaat gctccttcat caggcttaaa gaaaggtatg
    10081 agctgtttgt aagtcatacc aactagttca cttcctttgt attcagctat gatggaatat
    10141 ggaattaatt tatctcctgc tgtgtattcg gataatgata ggcttgaggc ttttccatta
    10201 aaatgggcag ataccaaatc tttagctaga ataacagttt gaggttcaaa ggtataaggg
    10261 ttataggttt gcacagccac atatgttatt gttgctccaa cacaaagggc tgtatttgat
    10321 ggcaaagtcc atggtgtagt agtccatgct aagaaatatg cttttccaaa ttgggttaat
    10381 tctgcaatag ggttatctat ttcaaattga gcaactacgg tagtatcttt tacatctttg
    10441 taacatcccg gttgatttag ctcgtgcgaa ctaagcccac taccagcagc aggcgagtag
    10501 ggttgaatgg tatatccttt gtataataaa tttttattgt acaattcttt taataaccac
    10561 caaatagatt caataaattt attatcataa gtgatatatg gatggttcat atccacccaa
    10621 taacccattt tgtgggttaa actttcccat tctttggtat atttcatcac atccttacga
    10681 catgcggcat tgtactcttc tactgatata gatttaccaa tatcttcttt ggtaatacct
    10741 agagattttt ccactcctaa ttctactggt aagccgtgag tatcccatcc tgctttgcgt
    10801 tgtacttgat accctttcat tgttttgtaa cgcaaaaata aatctttaat ggtacgtgcc
    10861 attacgtggt gaatgccagg catgccgttg gcagaagggg gtccttcgta aaaaacaaaa
    10921 gtaggatttc ctgttcttgt atcaatactt tttttaaaaa tgtcatgttt atcccagtag
    10981 gcaagaatat ctttatttat tgccgagagg tcaagatgtg tatattctga aaattttgta
    11041 ttcatatcat tattttttca atttgcaaag ataaaaaaac aacttagaac aaaaaaagat
    11101 atttttacaa aaaagcttct tcttttatag ataagaagcc tttttgtagt taacgatatg
    11161 tcatttttta gcgtatccta gaaagggatc gtatcaaagc ttcatcttta ccgatggctc
    11221 taatagctaa atagcagaga ataagattga taacaggtat gataattggc aatttgtaat
    11281 caataatgct gttcttgcta atgatagtag tggcaaaatc aatataaaaa tagattactc
    11341 cgtagagaaa aatcatcaat atcattgcta taacatttaa tcgcatctga agtattcttt
    11401 ttttgtatat aaaaatactt gaaaaagaaa ttataggaag taataatact aaaaataaat
    11461 agggaataaa ttgatatatg gtttgatttg attcagtgag ttgaataccc cacgtggtta
    11521 tattatacac atgtatgtca tgtgtaatag atgcaattgg aaaaaaccaa aggcatactc
    11581 ctaatatgct aataattaat aaatatacgg attgaatgcg ttgtatcata gtagaattag
    11641 aatagaaatt agtatgttca taaaaaaagt ccgtagcttt agcagcacac ggactttagt
    11701 tttgttggaa gtgtaaatta ttcagcaact actgcagctg aatcaacaac tacaacagct
    11761 gaatcaacaa caactgcagc tgaatcaaca acttcaacaa cttcttcaac aacttcttct
    11821 actacagctt cttcagtagc tttttctgca catgaagcaa aagcaacagc aacgatagta
    11881 gcaacaaata atactaactt tttcattttt ttagttttta aaatattaaa caatgatttt
    11941 ttttgaacgc acaaagatat ccatttattt tgtaacaaaa ataacaaaaa taaaaaaaat
    12001 aaaaaaactc ttgttaataa gtgttaataa tacgttagag agcaaatgta gtatacataa
    12061 cggtgtaaat aaatgataaa attccttaaa tactgatatt tgtatacaaa tagcgtttaa
    12121 tgctcctttt gggtgttttc tcaaattcat taggatatag gtgtattttt gacacatttt
    12181 cgtacgtacc tactacgttg tttagattgt tcttattttc ttccataata atttctaaat
    12241 cttgttgtgt aagttgtgaa ccatccattg cttcgtagtc tgggtaaact agagcaataa
    12301 gtttaccatg tttttctatt actaaacttt ccataataaa tggtaaatta tttagttttg
    12361 cttctatttc ttcagggtat atattttgtc cattagcccc taaaatcatg ctttttgatc
    12421 ttcccttaat aaaaatataa ccatgttcat caatagttcc taaatcgcct gttttcaacc
    12481 atccatcgtc ggtgaatgct tcggatgtag ccagttcgtt tttataatat ccctgcatga
    12541 catgttctcc ttttactagt atttctccta caatattgta agggtcgggt gaatcaatct
    12601 taacttgcat tgtatctaat attttaccag ctgaggaagg tctaaattca gtatggcttg
    12661 catatgaaat aagtggagca cattctgtca ttccataccc aatggtaaaa cgaaacccaa
    12721 ttttagttaa gaattcttct acttctttgt ttaatgcagc accaccaaca acaatttcag
    12781 tgaattcgcc accaaatgat tcagtaagtt tcttatttac ttgttcataa atctttttat
    12841 ttagcaatgg gatatttaga gctagtttaa tacctgtttt atttaacgaa ggctggatag
    12901 attttttata tactttttct aaaataaggg gaaccataaa aataacgtta ggttttattt
    12961 cttcaaatgc ttttaacaaa acttttggac ttggtatttt attcaaaaaa tatatatgag
    13021 aaccagaaca tgtgggaaca agaaaatcga aagcacaacc ataggcgtga gctaatggta
    13081 aaaaagataa tactctatct ccttttttaa gtagctgggt tttgaaccca aaagtaatat
    13141 tccctgctag attattggct gttaacataa ctcctttact aaatcctgta gtacctgatg
    13201 tataatttag catggcaagt tcactgttat cgcgtgatgt gtattgtata tggctttggg
    13261 taaagccttg cggatatttc ttctcaaact tgctagttag attgcctact attttttgaa
    13321 ttcctgcacc atctttttga tgtatacatc gaaaatcgtc aatggaaaat gctgcttgaa
    13381 tattttccat tttatcttct tctaaattat cccaaatact atcactacaa aataaaaaga
    13441 cagtttcaga gtgattaaca atatgatgca cgtcattggg attaaaatct tgcaaaatag
    13501 gtacaattac ggcaccataa gttactgtag ctacaaatgt gatagcccaa tgtggtgtat
    13561 ttttgccaat caaagcaatt ttatctccag gcttaatttt agattctgca aacaaaaggt
    13621 gtaatttttc aattttcagt gcgacttctc catacgtcat gctagtaccc aaagtataat
    13681 ctgtgtaggc aggtaactcc caattttcct taaaactagt ttcaaagagc ttgataaaat
    13741 tttctttaat catagtattt tgatttatgt tgttttacaa aaataaacta ttttttgata
    13801 catatagttt gtaggctaac ttttaagaaa ttttgttagt aaaacaatgc tacatgtttt
    13861 cttctttttt tgctagttgc catatttctt ccattttggc aagaggcata tcactaagag
    13921 ctatttcatg ttgttttgca tattcttcta agtaggtaaa acgctgaata aattttttat
    13981 tggtatgttc taaggcatta tctgggttaa ttttatacaa tctagcagca ttaattaaac
    14041 tgaaaaatac atcaccaaac tctttttcca ttttttcttt gttcattgag tctatttcca
    14101 cggttaattc tgaaatctct tcttgcactt ttttccacac atcctctttt ttttcccagt
    14161 caaaaccagc acttcgagct ttatcttgga tgcgatgagc ttttataatg gacggaagag
    14221 aggatggaac gcccgacagt atcgttttgt ttccttcttt ttcttttaat tttaattttt
    14281 cccaattttt ttctacctct tgagaggttt gagcttgttc tgtcccaaaa atatggggat
    14341 gtctgtatat aagtttttcg gcaagtgcat cacagatatc ttttagtgta aaagctgatt
    14401 tttcttctcc aatttttgcg tagaataata tatgcagtaa taaatcgcct aattcttttt
    14461 ttatttgttg gttgttgcct tgaagtatag catctgacaa ttcgtacgtt tcttcaatag
    14521 taagaactcg aagggtttcg ttagtttgtt ttttatccca aggacattta actcttaatt
    14581 catctaatat atgaatgacg ttttcaacag cttctaattt ttttttcata cttatattct
    14641 tatttacgat ttctcgatag ggcagtacaa aactcgtaaa taatatttag ttaaacaaat
    14701 ttcttctaca tcacttatcg acttttagtg cttaaattct tgcataaatg cacgcaacgt
    14761 tacaaatatg aaattctagg gagtttaaat acgtgataat tgtatgattt ttaagtattt
    14821 atgtatgtaa tttgttatta acaatataaa caaaatagga aatggtacat aattttaaaa
    14881 taatggcatc acgtaagtct attttaacag attattgatt acatatgtaa tataacaaga
    14941 tgttaaagtt aattacatat taattttagg aaaacataga gtagggtaat cgtttatttt
    15001 cacattgaat tacatttgta tttttaatag aatcatactc tagattaaaa taatataata
    15061 attatcaatt tattgtatat tagatattta taaatttatt atgaagtatt tatttatatt
    15121 taatggtttt tacct
//
LOCUS       DAOSRP010000033        15133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4029_length_15133_cov_4.831808, whole genome shotgun sequence.
ACCESSION   DAOSRP010000033 DAOSRP010000000
VERSION     DAOSRP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_15133_cov_4.831808"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLJ82_03360"
     CDS             <1..489
                     /locus_tag="PLJ82_03360"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOR40578.1"
                     /translation="VNVMVGMWDDYWYNTNNFYFYFDTNGKFYFIPYDYDNTLGTSSG
                     MNSGKQDMLNWGALDDSRLLMKKIMSITAFKNQYKAYIKELANANNDLFDTNKSIARI
                     KQWHAMIGNYIVNDTKEDMLIEDKPASWGNAGYYRLFTGDDKGESGDANFFKTKIKSI
                     TW"
     gene            561..1337
                     /gene="surE"
                     /locus_tag="PLJ82_03365"
     CDS             561..1337
                     /gene="surE"
                     /locus_tag="PLJ82_03365"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOR40579.1"
                     /translation="MKNRPLILVTNDDGVDARGIHSLIEAVSSLGEVVVIAPDGPRSA
                     QSNAITVNSPLRFWKHLEKDGLTVYRCNGTPTDCIKLGLCNILDEKPDIIVSGINHGS
                     NSSASVMYSGTMGGVFEGCRAGIPSVGFSLCNYDPRADFTVAMQYAYMISETVLVEGL
                     PTHTCLNVNVPPIQDVKGYKLCRQADGYWHEKFVKREDPYGNNYYWLTGNYVNNEPQA
                     TDTDEAMMAQGYVSVVPCKIDLTDYDALEELKRWNYKAKV"
     gene            1619..2755
                     /gene="lpxB"
                     /locus_tag="PLJ82_03370"
     CDS             1619..2755
                     /gene="lpxB"
                     /locus_tag="PLJ82_03370"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOR40580.1"
                     /translation="MRYYIIAGEASGDLHASNLVKSIFKQDNQAVIRGFGGDSMQNEG
                     VQLVKHYRELAFMGFIPVLLNIRTIFRNLSFCQQDITDFKPDVVILIDYPGFNLKIAK
                     FVKLHLKLPVFYYIPPKIWAWKEYRIKAIKKYIDKVFTIFPFETEFYAKHQCEVQYVG
                     NPSVESVHTYQATAPNRSLFIQQNALDDKKIIALLPGSRKQELEACLPIMLQVDFSQF
                     ADYQFIVAGTDALPCTLYHTLMKGSNAKIVYGKTYDLLQHAEAAIVTSGTATLETALF
                     NVPQVVCYEAFLSRLVAPFLRMVLKIKYVSLVNIIAQKELVKELLIQDFTPKNTQKEL
                     QKLVFDQAYRANILAGYQQLHTELGTTIASDTAAKAMVNYLKKE"
     gene            2767..3162
                     /locus_tag="PLJ82_03375"
     CDS             2767..3162
                     /locus_tag="PLJ82_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOR40581.1"
                     /translation="MPVTLHHIGIWVDDLDKMRQFYEHYFSAHCSKKYINPITNFSSY
                     FLSFGNDSTRIELMCRVDTTIEPTKRGIMKGYAHIALSVGSTSKVNQLTENLRNDHYK
                     IVSEPRTTGDGYYESIVLDPEGNYIEITV"
     gene            3400..4797
                     /locus_tag="PLJ82_03380"
     CDS             3400..4797
                     /locus_tag="PLJ82_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOR40582.1"
                     /translation="MKTLYFQISTFILVIVLMASCKTTLQIEPPRESYLPSNLTPATS
                     ELPLLVEVDVKKLESAINQKINGLVFEGDKLNNQDLSIKIWKAQNFSFNVKNNVIEYR
                     VPLKLWTRFAWSVDKFGLSMGDTYEATGSIALNYKTTIEIDKNWKLLAKTTSHGFQWI
                     DTPKLNVIGVSVPITPIATIALQQSEELITSQIDAILAQTINIKKYVAMAWKEAQKPM
                     QVSPENDLWVRISPKNVYVSPFTTNGQKLYVAISIQAVIESFMGVQPVQNKLTVLPPF
                     TVSANQPKDFNLNIATDITYEKISELAKSQLQNKTFSEGNKSITITDLSVFGSEGKAI
                     FVADVIGSIKGRIYFTGTLGYNAETMSIEVLQPEFDIKTKNALVKSANWLLNGFIIKK
                     ITPYLTYPVQDELTTMKTEANKMLTNYKVYDGVDLTGKLHTISVQRIDLVPGAIRLHA
                     NVGGNIALKVNDIHF"
     gene            complement(4891..6531)
                     /locus_tag="PLJ82_03385"
     CDS             complement(4891..6531)
                     /locus_tag="PLJ82_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR40583.1"
                     /translation="MILAEGLTVEFGGTSLFDNISFVVNQKDRIALMGKNGAGKSTLL
                     KILAGVREPSRGKVTTPKDYEIAYLPQHLMTEDSRTVFEEASQAFSSVFEMEKKINDL
                     NEQLAIRTDYESDSYMALIEEVSALSEKFYSIEEINYDGEIEKILLGLGFSRTDFNRK
                     TSEFSGGWRMRIELAKLLLRNPQAILLDEPTNHLDIESIQWLENFLIQSDKAVIVISH
                     DRAFVDTITNRTIEVTMGQIYDYKATYSHYLELRKERRQQQQKQYEDQQKMIAETTEF
                     IERFKGTYSKTLQVQSRVKMLEKLQIVEVDEEDTSALRLKFPPSPRSGNYPVIAENVS
                     KQYDDKLIFKDVNVMIHRGEKIAFVGKNGEGKSTLVKCIMGEIPFGGTLTLGHNVKIG
                     YFAQNQASMLDENLTVFQTIDDIAVGDIRTKIRDILGAFMFGREDIDKKVKVLSGGER
                     TRLAMIKLLLEPVNLLILDEPTNHLDLKTKDILKQALIDFDGTLITVSHDRDFLDGLA
                     TKVYEFGNQKVIEHHEGIYEFLEKKKLTNLKELERNKK"
     gene            complement(6617..7570)
                     /locus_tag="PLJ82_03390"
     CDS             complement(6617..7570)
                     /locus_tag="PLJ82_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOR40584.1"
                     /translation="MIINILILAFGLLILVKGADWLVDGASALAKKHHISDLAIGLTI
                     VAFGTSAPELVVNVFAAADNFPAIVFGNIIGSNLFNLFAILGITGLIMPIAVQSSTVW
                     KEIPISFLAIVVLFILGNDFYFQHTGTVSRLDGILLLLFFAGFLWYVYKQLKTDSNAM
                     ETSQVVLPPWKIYTFIAVGLGALVLGGKLVVNSAVNIANVLGISQKVIGLTIVAAGTS
                     LPELATSVVAAVKKNNDIAVGNIIGSNIFNIFLIIGVSAIIRPLNYIPTFNIDLYILA
                     TGTLLLFIAMFIGKKKKLDRWEAAFLLSAYIGYVVYLIAKI"
     gene            complement(7679..8086)
                     /locus_tag="PLJ82_03395"
     CDS             complement(7679..8086)
                     /locus_tag="PLJ82_03395"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HOR40585.1"
                     /translation="MPTTAPYSIILFDGMCYACSFVVRLILLLDRKKHFRFAPLQSEI
                     GEKLSSSYTITTPTATILFISQGNCYTKSTAILEIFKILGGIWLLLLIFRYIPLRVRD
                     KLYDWIAKNRYKLFGKRTTCSIPPPRYQDRFLK"
     gene            complement(8110..8703)
                     /locus_tag="PLJ82_03400"
     CDS             complement(8110..8703)
                     /locus_tag="PLJ82_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOR40586.1"
                     /translation="MLLKDVLHKYSVVLVSNSPRRRELLKGLEINFTSTSVNTDESYP
                     IELTGSAIPIHIAREKAKAYSRQLQSNTLAITADTVVLVDGEVLGKPRYAEEAAAMLR
                     KLSGKTHEVITGVCLFTQQKMHTFAGTSHVTFRALSEEEIIHYVKLYTPMDKAGSYGV
                     QEWIGYIGIEHISGSYYNVMGLPTALLYNELLAFVKS"
     gene            complement(8717..9673)
                     /locus_tag="PLJ82_03405"
     CDS             complement(8717..9673)
                     /locus_tag="PLJ82_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOR40587.1"
                     /translation="MKKFIQLIRLQNIISLLIFQALMYYGVVVAILTKYGLSFSYISL
                     EILLLFIASAFIAAAGYVVNDYFDTKIDTINKPDKVIIGKTLSRQQAAWIFQSLFGIG
                     ILAGIVLAFLLKDVSVGFIFIVIPGLLWFYSASYKRQFLLGNLIIGLCAAVSLFLVAY
                     IQSLLLVKQYGELIHYTQIIPEILQWVGGFSLFAFLLTVLREIVKDMEDVEGDREMEC
                     RTVPIVWGIAKTKIVAYVLGAVTIALLAYFSFFKIQFANDNVTERYFLFGVLMPFFVF
                     FYLLFKAKGKSDYHQISTFVKFMMFLGVLYSLFLPYLMTINA"
     gene            complement(9697..10224)
                     /locus_tag="PLJ82_03410"
     CDS             complement(9697..10224)
                     /locus_tag="PLJ82_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOR40588.1"
                     /translation="MNKNFKEYLPHIKAFAFDVDGVLSANCIPLYPNGEPMRMANLKD
                     GYVLQLAIKLGYPIAIITGGKTAAVRTRFEGLGIQDIYLGSSIKTIDFEHFIAKHGLH
                     PTDVLYMGDDMPDLPVMKLVGVPTCPSDAAAEIKAISCYVSNYRGGEGCVRDVIEQVL
                     KAQNKWLTDEHAFGW"
     gene            complement(10221..11006)
                     /locus_tag="PLJ82_03415"
     CDS             complement(10221..11006)
                     /locus_tag="PLJ82_03415"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOR40589.1"
                     /translation="MIDVVLIGAGNVATHLAIGLHKRGIHIRQIFSRTTTSAQLLAEQ
                     VGATFTSSKSAIYTDASLYICSVKDSEISTVLTNIDFHNKLLIHTAGSVALDILAPFS
                     ANYGVLYPLQTFSKSKPINWEQCSLFIEANNEKNAEFILQIASLLSTKVQIIDSEKRK
                     NLHIAAVFACNFTNHLYTLASELMLGSGANFNDLLPLIEETTNKLCTLSPYEAQTGPA
                     VRQDTNIVAEHIAQLAKTDINKATIYALLSKNIMQTHKMKNKE"
     gene            complement(11010..11546)
                     /locus_tag="PLJ82_03420"
     CDS             complement(11010..11546)
                     /locus_tag="PLJ82_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR40590.1"
                     /translation="MKSMYHLLQHRRSCRKYQPTSVSAQDIDTLLHAALMSPSSKRIN
                     PWEFIVVTDKELLEKLSHSKEHSSSFIAEAPLAIVIMANIEKSDVWVEDCSIAAILIQ
                     LVAEDLGLGSCWVQIRNRFTKNHKTSSNDYVKEVLHIPDTYAVEAIISIGYKVDERKP
                     FDEEKLQTEKIHYQHFTH"
     gene            complement(11560..12213)
                     /locus_tag="PLJ82_03425"
     CDS             complement(11560..12213)
                     /locus_tag="PLJ82_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOR40591.1"
                     /translation="MNTFFKSILIATVVFFNTNTIYSQFQKEISVGIIQGTVYPTVNF
                     DPIVTQEPILRYHGGLAIRYIAEPNVGIQAELTYSQRGWNEQSSSTGSFLKEVDYIEL
                     PLLTHIYVGKKSFRFFMQLGPKIRYAIQEKDKNTYLNSSSYQHINPIDNYFDYSLLGG
                     LGIEIRTHKLGFVQIETRYDFGIGDMYSNRKSDYFARSSNQAILASINYFFPSFYVK"
     gene            complement(12219..14042)
                     /locus_tag="PLJ82_03430"
     CDS             complement(12219..14042)
                     /locus_tag="PLJ82_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOR40592.1"
                     /translation="MRKLIVFLVASFITFFVIASNPNVSKQVTVNGIQYYQYTVQKSE
                     GFYAISTKFGVTQDDIILANPSTKEGLRVGQEILIPIKTEAKKENYLLHTVEKGETVF
                     SISQKYKISKEEIYSLNPETREVLKIGSTIKIKSNATTENKPTNPSPTSIPQKTSVTD
                     KPIQQISDNKTENTYIEHRVRRRETLYSISELYDVEIDTIIKENPSVVNGLIVRSILR
                     IPMGKKAQQVEAITEKAFEEKPIAPIPQTSVVANKQDKKTHIKIAVLMPFELEATVRD
                     AGMDRFVEFYQGILIATDSLAQHGITIEVEAHDIGKIQNQLTKTLGIPSLKEVDIIFG
                     PAFSSQIPALANFAKLHKIKLVVPFSPTVPEIESNEYLYQVITPQSDLHTSIAKEFSK
                     VFSNKLVYFLQPRKDDLQDKKEFVNQLKVAFNQNKVSYRTGFLGDFSASRLDSIANST
                     NKELVLIVPSTNLVVVTQLSNMIQSVQSSSIRIFSFSEWHQLQIQDLYQLPHYTYTNY
                     YIDFKDSNIHAFFTKLRNTYGIAGTQSVPNYALLGYDLANYFIGLLSNYGSNFESKLQ
                     EKNNGLLQMNLQFEKVSVNGGYQNKGIFLQSYDQQGITTLK"
     gene            14279..>15133
                     /locus_tag="PLJ82_03435"
     CDS             14279..>15133
                     /locus_tag="PLJ82_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40593.1"
                     /translation="MKKRFIVLIVGLLSYFVVSAQFSVTGVDVVEYPQSDIAAVFVVD
                     AFTDGVLSYTLPENKDVTWSYYDESGNKTIIKSEIQVNKTSIPLQNNSSLGYELDIQG
                     ELQYVWVFHYPDFEVQLDDIQIDTNTEDRCQFIQAQLFITANSMKYQGYNQQTMPYEV
                     VRTFELAYDSAYFSQTTYEVESVVRKIEQTSYVQLTSPLMNTLYTLSGDQFARAFNRT
                     KEISSAQVEAFAVQINPTASIQTRDGLNEKERDLSSSKTLRGSAPLEVEVLSYANVPA
                     ARYFDWCLS"
BASE COUNT         4907 a         2538 c         2698 g         4990 t
ORIGIN      
        1 gtaaacgtta tggtgggcat gtgggacgat tattggtata atacaaataa tttttacttt
       61 tattttgata ccaatggcaa attttatttc ataccttacg attatgacaa taccttgggt
      121 acttctagtg gaatgaactc aggaaaacag gatatgctta attggggagc attagacgac
      181 agtcgtttat taatgaaaaa gataatgtct attactgcat ttaaaaacca atacaaagcc
      241 tatatcaagg aactagcaaa tgcaaataat gatttatttg ataccaataa aagtattgct
      301 cgcattaaac aatggcatgc aatgattggt aactatattg taaatgacac gaaagaagat
      361 atgctaatag aagataaacc tgccagttgg ggtaatgctg gttattatcg cttatttaca
      421 ggtgatgata agggagaatc tggtgatgct aattttttca aaacaaaaat taagagtatt
      481 acgtggtaat tatggtttat taatttcgta ttcttgttac ttattcttac atttgtgcat
      541 acaacaaaat agtaatacat atgaaaaata gaccattaat attagtaaca aatgatgacg
      601 gagtagatgc aagaggtata cattcactga ttgaagcggt aagctcttta ggggaagttg
      661 tggtgatagc acccgatgga ccacgttcgg ctcaatcaaa tgccattact gtcaattctc
      721 cattacgttt ttggaaacac ctagaaaaag atggattgac agtgtatcgt tgtaatggaa
      781 ctcctacaga ttgcattaaa ctagggttgt gtaatatatt agatgaaaaa ccagatatta
      841 ttgtttcggg tattaaccat ggttctaatt cttctgcgag tgtgatgtat tcaggcacta
      901 tgggtggagt atttgaaggt tgtagggctg gtattccttc tgttggtttt tcgttgtgta
      961 actacgatcc acgtgccgat tttacggtag caatgcaata tgcctacatg atttccgaaa
     1021 ccgtattggt agaaggattg cctacacata cctgcttaaa tgtaaatgtg ccacctattc
     1081 aagatgtgaa aggatataaa ttatgccgtc aggcagatgg atactggcac gaaaaatttg
     1141 taaaaagaga agatccttat ggtaataatt actattggct tacaggtaat tatgtaaaca
     1201 atgagccaca agcaaccgat accgatgagg ctatgatggc acaaggttat gtatcggtag
     1261 taccatgcaa aatagatttg acggattatg atgcattaga agaactaaaa cgatggaatt
     1321 ataaagcaaa agtttaatca ttttttatgg ggatttattc ctggtttttt atttccagtg
     1381 ctattgtttt tggttacttg gggaagtatt tacaaaggag aatttacttt ttgggatagt
     1441 gtcgtgcgta tgtatggcac ccatttgatg caacaatata ttttgttttg catgttacca
     1501 aatttattat acatcttctt tgcctacaaa acagacagat ggaaaacagc aagtggtgta
     1561 attgtagctc ttgttccgta tttgagtttg ttgtttatga atatctaata gttacaccat
     1621 gaggtattat attattgcag gcgaagcatc gggggattta catgcgtcaa atttagtaaa
     1681 atcaatcttt aaacaagata atcaggcagt tatacgtggt tttggtggcg actcgatgca
     1741 aaatgaaggt gtacagcttg taaaacacta tcgtgaattg gcgtttatgg gctttattcc
     1801 tgtattatta aatattcgta ctatattccg aaacctaagt ttttgccaac aagatattac
     1861 tgattttaaa cccgacgtag ttattttaat tgactatcct ggttttaatc tcaaaatagc
     1921 caaatttgta aaactacatc taaaattacc tgttttttac tatataccac caaaaatatg
     1981 ggcatggaag gaataccgta tcaaggctat caaaaagtat attgataaag tttttaccat
     2041 ttttcctttc gaaactgagt tttatgccaa gcatcaatgt gaggtgcaat atgttggtaa
     2101 tccatcggtt gaaagtgtgc atacatacca agcaacagca cctaatcgca gtctttttat
     2161 acaacaaaat gccttagatg ataaaaaaat aattgccttg ctaccaggta gtcgtaagca
     2221 agagttagag gcttgtttgc cgataatgtt gcaagttgat ttttctcaat ttgctgatta
     2281 tcagtttata gtagcaggta ccgatgcgtt accttgtaca ctgtatcata ccttaatgaa
     2341 agggagcaat gctaaaatag tgtacggaaa aacctatgat ttattgcaac atgccgaagc
     2401 agcgattgtt acctcgggca cagcaacctt agaaacagct ctttttaatg tgccacaagt
     2461 ggtgtgttat gaggcttttt tatcacgttt ggtagctcca tttttacgaa tggtgttgaa
     2521 aattaagtat gtgtctttgg taaatattat tgcccaaaaa gaattagtaa aagaattact
     2581 tatacaagat tttaccccaa aaaatacgca aaaggaatta caaaagctag tattcgacca
     2641 agcatatcgt gctaatatat tggcaggcta tcagcaatta catactgaat taggtacaac
     2701 aattgcctct gatactgcag caaaagcaat ggttaattat ttgaagaaag aataaataaa
     2761 tagccaatgc cagttacact ccatcatata gggatttggg ttgatgactt ggacaaaatg
     2821 cgtcaattct atgaacatta ttttagtgct cattgtagca aaaaatatat caatcccata
     2881 acgaatttca gttcctattt tttgtcgttt ggtaatgata gtactagaat agagttaatg
     2941 tgtagggttg atacaaccat tgaacccaca aaaagaggca taatgaaagg ttatgcacac
     3001 atagcactat cggtgggtag tacaagtaaa gtaaatcaac tgacggaaaa cctacgcaat
     3061 gaccattaca agattgtaag tgaaccacgt actacaggtg atgggtatta tgaaagcatc
     3121 gtactcgacc cagaagggaa ttatattgaa attactgtct aacattctac cttactactg
     3181 tctgattttt ttttaattag gtttaaaaat atacgatcaa aagggaacat aatacaagca
     3241 tatcctaagg gaaattgctc tcaacggtat cgaataccga taaaaattcc acaaaaaaga
     3301 atagaaaaag aagaatgccc acattcgttt aagtaataga aaatgattat ttttatcatc
     3361 tgtattttta tgcatgaata ttaatataac atatttatta tgaagacatt atatttccaa
     3421 ataagtacat ttattttagt aatagttttg atggcatcat gtaaaacaac cttacaaata
     3481 gagcctccta gagagtctta tttaccatca aatttaacac cagccacatc tgagttacca
     3541 ttattagtag aggtagatgt aaaaaaatta gaatcagcta ttaatcaaaa aataaatgga
     3601 ttagtttttg aaggagataa actgaataat caggatttga gtataaaaat ttggaaagca
     3661 caaaattttt cgtttaatgt gaaaaataat gtgattgaat accgtgtgcc actaaaactt
     3721 tggacacgtt ttgcttggtc agtagataag tttggacttt cgatgggcga tacatatgag
     3781 gctactggga gtattgcact gaactataaa acaactattg agattgataa aaattggaaa
     3841 ttactagcca aaacgacctc gcatggtttt cagtggattg atacaccaaa actgaatgtt
     3901 ataggagtaa gtgtacctat aacacccatt gctactattg cacttcagca aagcgaagag
     3961 ttgataacaa gtcagattga tgctatattg gcacaaacga taaatataaa aaaatatgtt
     4021 gctatggcat ggaaagaagc acaaaagccc atgcaagtaa gtcccgaaaa tgacctatgg
     4081 gtgcgtatta gtcctaaaaa tgtgtatgtt tcaccattca caaccaatgg acagaaatta
     4141 tatgttgcta tttctataca agcagtaata gagtcattta tgggggtaca acctgttcaa
     4201 aataagctca cagtgctacc gccctttacc gtatcagcaa atcagccaaa ggattttaac
     4261 ctgaatattg ctactgatat aacgtacgaa aaaatttcag aattagctaa atcgcagttg
     4321 caaaataaaa cattttcgga aggaaacaaa agcattacaa taactgacct ttcggtattt
     4381 ggtagcgaag gaaaagctat ttttgttgct gatgttattg ggtcgataaa aggtcgtatt
     4441 tactttacgg gaacgttagg atacaatgcc gaaacaatgt caattgaagt attacaacct
     4501 gagtttgata ttaaaactaa aaatgctctt gtaaaatcag caaattggtt gttaaatgga
     4561 ttcattataa aaaaaataac gccttatttg acatatcctg tgcaggacga acttacaaca
     4621 atgaagacag aggcgaacaa gatgctcaca aactacaagg tgtatgatgg tgtagacctt
     4681 actggcaagt tacacacaat atccgtgcaa agaatcgatt tagtgcctgg agctatacgt
     4741 ttgcatgcga atgtaggtgg aaatattgcc ctaaaagtaa acgatataca cttctaaatc
     4801 gggagtgtaa acatataggc agtccaaaca aagagctgta aatatataaa gttgagttat
     4861 gccatagcaa tagaacttgg ctactagtag ctatttttta ttacgctcca actcttttaa
     4921 gttagttagt tttttctttt caagaaattc gtaaatacct tcgtggtgtt ctataacttt
     4981 ttgattacca aattcatata ctttcgtagc cagaccatct aaaaaatcgc ggtcgtgcga
     5041 aaccgttatt aacgtgccat caaagtcgat gagggcttgt ttcaatatat cttttgtctt
     5101 caagtcaaga tggttggtag gctcatctaa tatcagcaag tttacaggtt cgagcaacag
     5161 ttttatcatg gctaatcgtg tccgttcgcc acccgaaagt acttttactt ttttgtcaat
     5221 atcctctcta ccaaacatga aagctcccag tatatcgcga atctttgtac gtatatcgcc
     5281 cacagcaata tcatcaatgg tttgaaaaac ggtaagattt tcgtctaaca ttgaggcttg
     5341 attttgagca aaatacccaa tttttacatt gtgtccgagg gtgagtgtac cgccaaaagg
     5401 aatttcgccc atgatacatt ttaccaaggt tgatttacct tcgccatttt tgcctacaaa
     5461 agcaattttt tcgcctcggt gtatcattac atttacatcc ttaaatatca gcttatcatc
     5521 atactgtttg cttacatttt cggctattac ggggtaatta cccgaacgtg gcgagggagg
     5581 aaatttgagt cgcaaagcag atgtatcttc ttcatcaact tctacaattt gcaatttttc
     5641 gagcattttt acacgcgatt gcacttgcag ggttttggaa taggtgcctt taaaccgttc
     5701 gataaactcg gtggtttcag caatcatttt ttgttggtcc tcatactgtt tttgctgttg
     5761 ctgacggcgt tctttacgta gttcaaggta gtgagagtag gtagctttgt aatcatatat
     5821 ttgtcccatc gttacctcaa ttgtacgatt ggtaatcgta tcaacaaaag ctctatcatg
     5881 cgaaatgaca ataaccgctt tatcactttg tatcaaaaag ttttccaacc attgaatgga
     5941 ttcaatgtcg aggtggttgg taggttcgtc gagcaagata gcttgtggat tgcgtaataa
     6001 taacttcgct agttcgatac gcatacgcca gccaccgcta aattcactgg tttttctgtt
     6061 aaaatcagta cgagaaaacc ctaatccaag caaaatcttt tcaatttcac catcatagtt
     6121 gatttcttcg atgctataaa atttttcact caaagccgaa acttcttcaa ttaacgccat
     6181 ataactatcg ctttcgtaat cggtacgtat ggcaagttgt tcgttgaggt cattaatttt
     6241 tttctccatc tcaaatacag aagaaaaggc ttgagacgct tcttcaaata ctgttcgaga
     6301 gtcttctgtc attaagtgtt gaggtaaata ggctatttcg taatctttag gagtggttac
     6361 tttcccacgt gatggttcgc gaacacctgc taaaattttg agtaaggtag atttaccagc
     6421 accattttta cccattaggg caatgcggtc tttttggttt actacaaacg aaatattatc
     6481 aaatagggaa gtgcccccaa attctacggt tagtccttcg gctaaaatca ttttacgtgc
     6541 atgtttaagg gcacaaaggt agtaaaaaaa tggttttgtt tactatttaa attcgtacta
     6601 aagaataatc ttaggtttat atttttgcaa ttaaatacac aacatatcca atataggcag
     6661 aaagaagaaa agccgcctcc caacggtcaa gttttttctt tttaccaata aacatggcaa
     6721 taaacaagag caaagtacct gtggctaaaa tgtataggtc aatgttaaaa gtcggtatat
     6781 aatttagtgg tcgtataatt gcactaacac caataatgag aaaaatatta aaaatattcg
     6841 acccaataat gtttcccacg gcaatatcat tgttcttttt tactgcagct actacagaag
     6901 tcgctaattc gggcaacgat gtgcctgctg ctacgatagt taatcctata actttttgac
     6961 ttattcctag tacgttggca atgtttacgg cactatttac tactaatttt ccacccaata
     7021 ccaaagctcc taatccaaca gcaataaagg tgtaaatttt ccatggtggt aatactactt
     7081 gagaggtttc cattgcatta gaatctgttt tcaattgctt gtacacatac cacaaaaaac
     7141 cagcaaaaaa taaaagcaat aggataccat ctaagcgaga aacagtacca gtatgttgga
     7201 aataaaaatc atttcctaaa ataaacaata ccacaatagc taaaaatgaa ataggaattt
     7261 ctttccacac tgtactagat tgaacagcaa tgggcataat gagtccagta ataccgagta
     7321 tcgcaaataa attaaataag ttgctaccaa ttatattgcc aaatacaatt gcaggaaagt
     7381 tatctgctgc ggcaaacacg tttacaacta actcgggtgc agaggttcca aaagccacaa
     7441 tagtgagacc tatagctaaa tctgaaatat ggtgtttttt tgccaaagca gatgcaccat
     7501 ccactaacca gtctgctcct tttacaagaa ttagtaatcc gaatgctaga attaagatgt
     7561 tgataatcat atggataaag atttttttat tgttgaataa attatcaaca aaggtatatg
     7621 attattggtg tattgcactc ttttttttgt aaaaaaaata gggattttaa ataaaaattt
     7681 atttcaagaa tctatcctga taacgaggtg gagggatgct acaagttgta cgttttccaa
     7741 atagtttgta gcggttttta gctatccaat cgtatagttt atctctaaca cgtaagggga
     7801 tataacgaaa gattaacagt aaaagccata ttcctcccaa aattttaaat atctcaagaa
     7861 tagctgtaga ttttgtataa caatttcctt gtgagatgaa aagaatagtt gcagtaggcg
     7921 tagtgatagt gtaggatgaa gataattttt ccccaatttc agattgtaag ggagcaaaac
     7981 gaaagtgttt ttttctatca agcaacaaaa ttagacgcac aacaaacgaa caggcataac
     8041 acatgccgtc aaataatatg atagaatatg gggctgttgt tggcatatca tagataataa
     8101 ataacctgcc taagacttaa caaatgctaa taattcatta tataaaagag ctgtgggcaa
     8161 acccattaca ttgtaatatg aaccactaat gtgttcgatg ccaatatatc caatccactc
     8221 ttgcactcca tagctccctg ccttatccat aggagtgtat agcttaacat agtgaataat
     8281 ttcttcttcg ctcaatgctc taaaagtaac atgcgaagtt ccagcaaatg tatgcatttt
     8341 ttgttgggta aataaacaaa cacctgtaat cacttcgtgt gttttaccag ataatttgcg
     8401 tagcatcgca gctgcttctt cggcataacg aggctttcct agtacttctc catctactag
     8461 taccaccgtg tcggctgtaa tggctagtgt gttcgattgc aattgacgac tgtatgcctt
     8521 ggctttctct ctagcaatat gtatgggaat ggcacttcca gttagctcta tcggatagct
     8581 ctcgtctgta tttacagaag tgcttgtaaa attgatttct aatcctttaa gcagttctct
     8641 ccgtcgtggc gagttggaaa ccaatacgac agaatatttg tgtaacacat cttttaataa
     8701 catataatct atgatattat gcgttgatag tcattaaata aggtaaaaac aagctatata
     8761 acacacctaa aaacatcata aattttacaa atgtactgat ttggtgatag tcagattttc
     8821 cctttgcctt aaataaaaga taaaaaaata caaaaaaagg cattagtact ccaaaaagaa
     8881 aataccgttc tgttacatta tcattggcaa actgtatttt gaaaaaagaa aaataggcaa
     8941 ggagagcaat agttactgct cctaatacat atgccactat ctttgttttg gcaatgcccc
     9001 acacaatagg tactgtgcga cattccattt ctctatctcc ctctacatct tccatatctt
     9061 ttactatttc gcgtagtaca gtaagtaaaa atgcaaacaa actaaaaccg cctacccatt
     9121 gcaatatttc aggaataatc tgtgtgtaat gaatcagttc tccatattgt tttactaata
     9181 acaaagattg tatgtaagct actaaaaaaa gtgatacggc tgcacacaat ccaatgataa
     9241 ggttacctag taaaaattgt cttttgtacg aagctgaata gaaccaaaga agtcctggta
     9301 tgacaataaa aataaaacct acgcttacat ctttcaacag aaatgccaat acgatacctg
     9361 ccaaaattcc aataccaaaa agggattgaa aaatccatgc tgcttgctga cgcgatagtg
     9421 ttttgccaat gattacttta tctggtttat taatcgtgtc aatttttgtg tcaaaatagt
     9481 cattgacaac atatcccgca gctgcaataa aagctgacgc aataaacagt aagagtattt
     9541 caaggcttat atacgaaaag gatagaccat atttagttaa aatggcaaca acaacgccat
     9601 aatacataag agcctgaaat ataagtaaac taataatgtt ttgtaagcga attaattgaa
     9661 taaatttttt catagtgtgt tattattatt tgttttttac caaccgaatg catgttcatc
     9721 ggtcaaccat ttgttttgtg cttttaatac ttgttctatc acatcgcgta cacaaccttc
     9781 gcctccacgg tagtttgata cgtagcagga tattgcttta atttctgcag ccgcgtcgct
     9841 agggcaggtg ggcacaccta cgagtttcat tacaggcaaa tcgggcatat catctcccat
     9901 gtaaagcacg tctgtaggat gtaatccgtg tttggcaata aaatgttcaa aatctatcgt
     9961 ctttatggat gaccctaaat atatatcttg tatgcctaat ccctcgaatc gagtacgtac
    10021 agcagcagtt tttcctccag taatgatggc aataggatat cctaatttta tagccaattg
    10081 taatacatag ccatctttta gatttgccat acgcataggt tccccattgg gatacagggg
    10141 gatgcaattg gctgacaaaa caccatctac atcaaaagca aatgctttaa tatggggtaa
    10201 gtattcctta aaatttttat tcattctttg tttttcattt tatgggtttg cattatattt
    10261 ttacttaata aggcgtaaat cgttgcttta ttgatgtcgg tttttgctag ttgtgcaata
    10321 tgttctgcta cgatatttgt gtcttgtcgt actgctggac ccgtttgtgc ctcgtaagga
    10381 gatagtgtac ataatttgtt tgttgtttcc tcaattaaag gtagtaaatc gttaaagttt
    10441 gctccacttc ccaacattaa ttcactagct agcgtatata aatgatttgt aaaattacaa
    10501 gcgaatacag ccgcaatatg taagtttttg cgtttttctg aatcaataat ttgtactttt
    10561 gtgcttaata gtgaagcaat ttgtaaaatg aattctgcat ttttttcgtt attagcttca
    10621 atgaataggc tacattgttc ccaattaatt ggctttgatt ttgaaaacgt ttgtaacgga
    10681 tacagtacac cataattagc agaaaaaggg gctaatatat ctaatgctac actaccagca
    10741 gtatgaataa gtaatttgtt atgaaaatcg atatttgtta agaccgtact aatttcgctg
    10801 tcttttacac tgcaaatata caacgaagca tctgtataaa tagctgattt agaagaagta
    10861 aatgtagctc caacctgttc tgctagtaac tgagcagagg tcgtagtgcg actaaaaatc
    10921 tggcggatat gtatacctct tttatgcaat cctattgcca aatgagttgc tacattgcct
    10981 gcacctatta gtacaacatc tatcatatat tagtgagtaa aatgttgata atgaattttt
    11041 tctgtttgca atttttcttc atcaaatggt ttacgttcgt ctactttgta accaatacta
    11101 ataatagctt ctactgcata cgtatctgga atgtgtaata cttcttttac atagtcatta
    11161 gagcttgttt tatggttttt tgtaaaacga ttacgtattt gtacccagca agagcccaat
    11221 cctaaatctt ctgcaaccaa ttgtattaag atagcagcaa tcgaacagtc ttccacccat
    11281 acatcgcttt tttctatatt tgccattata actatagcta acggagcctc ggcaataaaa
    11341 gaacttgaat gttctttgct atgacttaat ttttctaata attctttgtc tgttacaaca
    11401 ataaattccc atggattaat acgtttggat gatggagaca tcaaagcagc gtgcaataag
    11461 gtgtcaatat cttgtgccga aacggatgta ggttggtatt tcctgcaact gcgtctgtgt
    11521 tgaagtaagt ggtacattga tttcatgatt gttgttgttt tattttacgt aaaatgaggg
    11581 aaaaaaataa ttaatacttg cgagtattgc ttggttgcta gagcgagcaa agtaatctga
    11641 tttacgatta ctatacatat ctccaatacc aaaatcgtat cgtgtttcta tttggacgaa
    11701 tcctaattta tgagtacgta tttctattcc taaaccacct aataaactgt aatcaaaata
    11761 attatcaata ggatttatgt gttgataaga agatgaattg agatacgtat ttttatcttt
    11821 ttcttgtata gcatagcgaa tttttggtcc taattgcata aaaaaacgaa aagacttttt
    11881 acctacataa atatgtgtta acaaaggtaa ttcgatgtaa tctacttctt ttaggaaact
    11941 tccagtagac gaagattgct cattccatcc tcgttggcta taggtaagtt ccgcttgtat
    12001 cccaacatta ggttcagcaa tataccgaat agctagccca ccatgatacc ttagaatagg
    12061 ttcttgtgta acaatagggt caaaattaac agtaggatat acagttcctt gaataatacc
    12121 aacggatatc tctttttgaa attgcgaata tattgtattt gtattaaaaa aaacaacagt
    12181 cgcaatcagt attgatttaa aaaaagtatt catttttgct attttaaagt ggtaatgcct
    12241 tgttggtcat aactttgcaa aaatatacct ttattttggt atccaccatt cacagatact
    12301 ttttcaaatt gcagattcat ttgcaataac ccattgtttt tttcttgtaa ttttgattca
    12361 aagttactac cataattaga taaaagtcct ataaaataat ttgctaaatc atatcctaac
    12421 agtgcgtagt tagggacgct ttgtgttcca gcaattccgt atgtatttcg cagtttagtg
    12481 aaaaaagcat ggatattact atctttaaaa tcaatataat aattcgtata cgtatagtgt
    12541 ggtaattgat ataaatcttg aatttgtagt tgatgccact cagaaaaact aaatatacga
    12601 atagaggagg attgtacaga ttgtatcatg ttactcaact gtgttactac gactaaattg
    12661 gtactgggca caataagtac aagttcttta tttgtactat tagctattga gtctaatctg
    12721 ctagctgaga aatctcccaa aaaaccagta cgatagctta ctttattttg attaaaagca
    12781 actttcagtt gattaacaaa ctctttttta tcctgcaaat catctttacg aggctgcaaa
    12841 aaataaacta gcttattgct aaatactttg ctaaattctt ttgcaatgct tgtgtgtaaa
    12901 tcactttgcg gagttattac ttgataaagg tattcgttag actcaatttc tggtacagtt
    12961 ggtgaaaaag gtaccactaa ttttatctta tgtaatttgg caaaattggc taaagcaggt
    13021 atttgactgc taaaagctgg accaaaaatt atatctacct ctttcaacga gggtatacca
    13081 agggttttag ttagttgatt ttgtatttta cctatatcat gtgcctcaac ctctatggtg
    13141 ataccatgct gtgcgagcga gtctgtggca attagtatgc cttgataaaa ttcaacaaaa
    13201 cgatccatgc ctgcatctct tactgtggct tctagttcaa atggcatcaa tacagcaatc
    13261 ttgatgtgtg tttttttgtc ttgtttgttt gctactacac ttgtttgtgg tatgggtgct
    13321 atcggttttt cttcaaaagc tttttctgta atagcctcta cttgttgtgc ttttttaccc
    13381 attgggatac gaagaatgga acgaacaatt aacccattaa caacgcttgg attttcttta
    13441 ataatggtgt ctatttctac atcatagagc tcagaaatac tgtataacgt ttctctacga
    13501 cgaaccctat gttcaatata ggtgttttct gttttattgt cagatatttg ttgtatcggt
    13561 ttatccgtaa cggaggtttt ttgtgggata ctcgtaggag aagggttagt aggcttgttt
    13621 tcagtagtag cattagactt aatttttatc gtagaaccaa ttttaagtac ttctctggtt
    13681 tctggattta agctgtaaat ttcttccttg cttattttgt atttttgtga tattgaaaaa
    13741 acggtttccc ctttttccac ggtgtgtaat agataatttt ctttttttgc ttccgttttt
    13801 ataggtatga gaatttcctg tcctacacgt agtccctctt ttgtagatgg atttgctaga
    13861 ataatgtcat cttgtgtaac cccaaatttt gtggatatgg cataaaatcc ctcgcttttt
    13921 tgtacggtat attggtagta ttgtatgcca tttaccgtta cttgtttaga aacatttgga
    13981 ttagaggcaa tgacaaaaaa agtgataaaa ctagcaacca gaaacacaat gagttttcgc
    14041 ataacgacta ttattttttc atataaaatt gttacaatga ggcaaagata cataaaaact
    14101 acaaattgta ctgtctttta ggggaagaaa acacttgtta gtagcataaa aaatactatc
    14161 tttacacaat attttttaaa aaatcagttt aacaaaataa taacgacata ttattttgac
    14221 tttttacaaa taaagagtaa atgctcgctt ttttcaaaca taactaagca attataaaat
    14281 gaaaaagagg tttattgtac ttattgttgg tttgctttcg tattttgttg tttcagccca
    14341 atttagtgtt actggtgttg atgtagtgga gtatcctcag tctgatattg cagctgtatt
    14401 tgttgtagat gcatttactg atggtgtatt atcttataca ttgcctgaga acaaagatgt
    14461 tacatggagt tattacgatg aatctggaaa taaaactatt attaaatccg aaatacaagt
    14521 aaataaaact tccattccgt tacaaaataa ttcatctctt ggctacgaac ttgacattca
    14581 gggcgaatta cagtatgttt gggtatttca ttatcccgat tttgaagttc aattggatga
    14641 tatacagata gatactaata cagaggatag gtgtcaattt attcaggcac agttgtttat
    14701 cacggcaaat tctatgaagt atcaagggta caatcaacaa actatgccat acgaagtagt
    14761 aagaacattt gaactagcat acgattctgc ttatttttcc caaactactt atgaggtaga
    14821 gtcagtcgta agaaaaatag aacaaacttc gtacgttcaa cttacgtctc ctcttatgaa
    14881 tacactttat acattgtctg gtgatcaatt tgcacgtgca tttaatcgta ccaaagaaat
    14941 ttcatctgct caggtagagg catttgcagt acaaattaac ccaactgcaa gtatccaaac
    15001 gcgtgatgga ttaaatgaaa aagaaaggga cttgtcttca tcgaaaactt tgcgtgggtc
    15061 tgctcctttg gaagtggaag tactaagcta tgcaaatgtg cctgctgcac gttattttga
    15121 ttggtgcctc tcg
//
LOCUS       DAOSRP010000034        14928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4096_length_14928_cov_4.661265, whole genome shotgun sequence.
ACCESSION   DAOSRP010000034 DAOSRP010000000
VERSION     DAOSRP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4096_length_14928_cov_4.661265"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..3307)
                     /locus_tag="PLJ82_03440"
     CDS             complement(311..3307)
                     /locus_tag="PLJ82_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR40594.1"
                     /translation="MKKILLIASSLMSISAFAKNCGNNPHRIDKVHIPAQLSQYNLFG
                     STNDVIDVAFIVSPTKIKVTLKNATPYNQGDITLYTYNYAKKPTTESFRLGTKITPAF
                     TQKGNIITIDLASLNLNTNYLIDIKGNQSPVFLDHAIGGILDTYFDASNEQNLGVNLD
                     NNVATFKLWSPPAGRITIHFYDQNEQPIKRKVEMKPGKNGVWQAQVTAKNLGVKSLDG
                     LYYQYEVYAYGKSRLALDPYAKSMAAHSPKGTDKIGKAAIVDMKSPRATPANFVRKYT
                     NYDHIANDVDLVCYEVHVRDFTVQPGVVKPELAGTYKGFAEKTDYLKELGITHAQLLP
                     IQNFFTTDEKNRQFSDTDAPVSNYNWGYDPHNYFTPEGWFSSNAADPYARIRELRELV
                     QDLHSKGIGVIMDVVYNHTYLVETFENVAPGCYYRYNDTNGISAHSGAGPTVESRRKM
                     VQKLTVESLNHFVTEYLVDGFRFDLMGFIDHETMRLIRQEVGKTYNPNNINELVLQGE
                     AWVFSDLDTDAKATGLHAATTKLNHPKEYLNLGFFNDVSRDAFAGREHQKGFVQGSYG
                     EIDRCVTAILGGVKNFPMKGKAFATEVLTDDYNAFAENPAVCLNYLTVHDGFTLWDKI
                     NLSVNDPSGLKRAKVMKLATAMIFTSQGKVILHAGDEFLRTKPLAINDIEKTRAHKTP
                     YTNPEEGIEYFHENTYISNDYTNQLRWDRFTNEFSHIAVPMVEYYQGLIAMRRALPGL
                     RMFDGDNIRKGITFYGMDIKEGAQTSKPKTLGVTDFSDEKLSKLTLKFTNGPANSTYY
                     IAGEVYDKRTEANTTAATMYAVTFNQAGVGEITFSRDQINNFDLGKWGDSKNLNFKLV
                     KNPGTWESIATAYTGSGNNRLRPMDVDDTSTLTVDLSVVDFATGYVPRKVDTFIAYEI
                     DNTLENATAPHISNTNYSRIIVIHNADENAITVDIPTITNPQEWSVIADGNKAGITPI
                     KKDDTAVKIEMKKVTVPALSMAVIVK"
     gene            3699..3881
                     /locus_tag="PLJ82_03445"
                     /pseudo
     CDS             3699..3881
                     /locus_tag="PLJ82_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            complement(3927..4298)
                     /locus_tag="PLJ82_03450"
     CDS             complement(3927..4298)
                     /locus_tag="PLJ82_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOR40595.1"
                     /translation="MIIQFIKFCVVGASGLLVDFGITYLCKEKIKLNKYIANSLGFIA
                     AASSNYLLNRMWTFENTDPNISTQYLLFIIISLIGLGINNGVIYLVLRKRDYNFYIVK
                     VFAIAVVTLWNFFMNFFFTFQ"
     gene            complement(4399..6090)
                     /locus_tag="PLJ82_03455"
     CDS             complement(4399..6090)
                     /locus_tag="PLJ82_03455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOR40596.1"
                     /translation="MNKNKLSANQLFWILNIAWMLLNLLQASVTELANDEAYYVVFAK
                     HLDWGYYDHPPMIALFIYLGQWLSGELGVRLLTVLSQPIYIWLLWKLIAPATVEKKDV
                     WLFFIIALAMPILHIYGFVAVPDAPLMLFSALFLYFFVQYLQHTSWYYIPFMALAIAA
                     IGYSKYTGVLVVLFACAAYPKVFLRKGIYITATLSLLLMLPHLYWQYQHEWVSFQYHL
                     VGRNQDFNAFYIAEYVGNFIAVFNPFILPLVVWIGFKNRHQDELTLLILKRIALFFFL
                     FFFASCIRGHVQPQWFIFVTFSVIALVWQWARNHEKRYRYIRTVSIISIVILIGFRIV
                     AATNPFGMKAEVFNNKTSYTQIANATGNLPVIFMGKYTMAAKYSYYTGLDAHASPNIH
                     FRTSQYQLWDFDSNLLEKPVAIHVGGDYPNATHIQTDNGQFSFVIDSCYMPIRKVQIQ
                     WLNPYNTIASKREEKNMTLSIHNPYPYDIVIDSEKIQLHYIIRKPWSWVEYFPVDVEN
                     PLILTAFSTTNIQQKISIESEKAKPNETYETGFLLTKPPYIVWYNSPIYNIQIVP"
     gene            complement(6100..7314)
                     /locus_tag="PLJ82_03460"
     CDS             complement(6100..7314)
                     /locus_tag="PLJ82_03460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR40597.1"
                     /translation="MNKKRKYLLLVISLLLITIGGMVWQRWNVWFGNRPEMPFTSKPY
                     PHRILLTLGENTNQRILSWQHDSVIQQATVKLTKLHSTDTLVITAKGNIVRSRGGVTA
                     FYTAQLNDLSSNAQYAYQVLSGEKQTEWYQFKTTSTNSPFSFMYIGDVQDRDTSNTSK
                     QAFQYIYQHFPEADFWAFGGDQIERPMNSYWEAFMNDLDGIRQSTPIIAIAGNHEYLK
                     YAIRKIDDRFTYTFPYFINNHNNNHVFTFVYGNVRFILLDSNREPWYIWQQRNYLKEV
                     LKNNSSLFTVVMLHHPVLSNKSSWNNASIRLMMEPILSQYPVDLVLQGHEHGYARNSK
                     KDKSGTSKLPIYVTSHLSKKFYPSFKATKITDAIIPNTALFQHITVDNNILTLYSYTL
                     QGEKVDSVSIVK"
     gene            complement(7357..7806)
                     /locus_tag="PLJ82_03465"
     CDS             complement(7357..7806)
                     /locus_tag="PLJ82_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOR40598.1"
                     /translation="MELIDQITEDIKKAMLAKEKTVLDALRGIKKELLEAKTAKGGDG
                     TVSEEMGTKILQKMVKQRKESAQIYTEQNRKELADKELEEAEIIARYLPKQLSDEELT
                     AAIQTIISQVGATSAKDMGKVMGIATKQLSGKVEGKLISEKVKSLLQ"
     gene            complement(8184..9143)
                     /gene="ftsZ"
                     /locus_tag="PLJ82_03470"
                     /pseudo
     CDS             complement(8184..9143)
                     /gene="ftsZ"
                     /locus_tag="PLJ82_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            complement(9159..10466)
                     /gene="ftsA"
                     /locus_tag="PLJ82_03475"
     CDS             complement(9159..10466)
                     /gene="ftsA"
                     /locus_tag="PLJ82_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOR40599.1"
                     /translation="MNTSTPYILAFDLGTTKIIGAVAQKTTEGKLSIVAYEEEETKPT
                     TIKQGAIFNVADTAFIINSIVTKLRNKTKLNLTKAYIGIGGRYLRCKENTTKRTLGYN
                     EIVEQNLLDKLYQENFKLQSTEFDILDVITQEYLVDDEWVADPAGIACTQLEGRYKII
                     YTKSEINTGINKVKELLAEKSQLEIISTFSSPIATANGLISDDEKELGCMIIDFGGST
                     TSYSVFTKNYLREIGVIPLGAKTITNDIRELNLTEPQAEKLKQKLGNAMATLEVPDKR
                     IIIAKAAHEQEEKSIQSTFLAQIIEARASEILNFIITPLKQKGYIKQLGAGIIITGGG
                     AQLKNMRALVQSLSGVDVRTTVGVHLLTEDSISDDFKKPSYSQIIGLLSMGTENCVSE
                     TIEEKFIPSKKTKKTGKNLFVRFADSASQRLTDLFTDNSDTIQ"
     gene            complement(10477..11208)
                     /locus_tag="PLJ82_03480"
     CDS             complement(10477..11208)
                     /locus_tag="PLJ82_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40600.1"
                     /translation="MIKKIALIVVSVVFLSYLIYAAISFTHTPQEQVCSTIHIFKIDS
                     VDYNFIKRKEIEKQLTDNNIYPIGRKIDFAFTHAIEEKIKEMNYVKRVECYRTNHGDV
                     VIRIWQRQPIVRIITPFYSYYIDQDRKKMPISKDFTVILPIVSGNVTESFAKNELFDF
                     VTYITNDSFWNAQIDQIYIKENKKIELVPKVGLYTITLGTLDDYETKLKKLYAFYKTY
                     PEFALTDTYSNINIEYKKLLYATKK"
     gene            complement(11220..12596)
                     /gene="murC"
                     /locus_tag="PLJ82_03485"
     CDS             complement(11220..12596)
                     /gene="murC"
                     /locus_tag="PLJ82_03485"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOR40601.1"
                     /translation="MKSCQQNYYFLGIGGIGMSALARYFKQLGHQVGGYDATENTLTQ
                     KLESEGMEIHYNDSVNTIPVKYMDKENTTIVYTPAIPHDMSERVFFEKAGFTMLKRAE
                     ILGEITRSKKALCVAGTHGKTTTSTILAHILTQSSLGTNAFLGGISKNYSTNLLVNAS
                     SDIVVVEADEYDRSFHQLSPHMAIITATDNDHLDIYQDRENLLSSFQHFTSLIQENGC
                     LLLKKGIELVPSTKNSVKIYTYSANEVADFYATNILIENEKILFDYVTPTFTIEKIKL
                     GVPILINVENAVAAMTIAYLNGVTKEELQHAIETFKGIERRFDFQLQTENVRFIDDYA
                     HHPEELKASIQSIRKLYPNKSMCGIFQPHLYTRTRDFATEFANSLSLLDSVILLDIYP
                     ARELPIKGVSSKMIFKHIQCKNKSICSKEDLLEEIKKYNFDVLITLGAGDIDKMVPLI
                     TQQLKKQL"
     gene            complement(12678..13748)
                     /gene="murG"
                     /locus_tag="PLJ82_03490"
                     /pseudo
     CDS             complement(12678..13748)
                     /gene="murG"
                     /locus_tag="PLJ82_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
     gene            complement(13758..>14928)
                     /locus_tag="PLJ82_03495"
     CDS             complement(13758..>14928)
                     /locus_tag="PLJ82_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOR40602.1"
                     /translation="QEAFRRSNHLAPFFSHIQMLGLGLIAMIIVHRFPYRYIRGLNYL
                     ILPIALVLLVLLPFIGVEANNAIRAIKVLGFEFQPLELAKFSTIVFISDMLARNQKEG
                     QTADKAFWPIISVLGIFCLLIAPQNLSTAVMLFGVGILLMMIGRISFAKLGITIGVLA
                     ALLLSLYVISHYFQPQFLGRLNTWVGRIDDFVSEINEPSAEKTYVITDDNMQIMHSKI
                     AIAHGMTPAGPGNSTQRDFLPLAFSDFIYAIVIEEYGLAGGIFIMLLYLIILFRSGRI
                     ATQCDKAYPTFMVVGLSLLIVIQAFISIAVASHLGPVTGQPLPLISRGGTSIIMTSVY
                     LGIILNISQYIKKEEKVSNNTTNAPADEAQNEVVSDSEDTAEKASDTETRQTDIK"
BASE COUNT         4276 a         2629 c         2760 g         5263 t
ORIGIN      
        1 tcaaatccct tgtttgtaat atgtgtatat atctccgttg ttttactact cgaatcacct
       61 atataaactt tgaatatatc ttaagtctgt cctccacctt ctccatcaaa caaccactct
      121 ttcggtttat attccttaaa atagtggggt aaagttatta aagttttttt aggtagcaaa
      181 gtgtatcggt cttttttttt gttgttcgta ttgtggtttt ttctgtttca caggcaatag
      241 ctatctccat caaatcaaaa aggcaagata acgaagtatc ctgcctttat tgaattcaaa
      301 aaaaatagta ttattttaca atcaccgcca tgcttagtgc aggtactgtt actttcttca
      361 tctctatttt tactgctgta tcgtcctttt taattggggt gatacctgct ttgttgccat
      421 cggcaattac gctccattct tgtgggttgg taattgttgg gatatccaca gtaatggcat
      481 tttcgtctgc attgtgtatt acgataatgc gtgagtagtt ggtgttgctg atgtgtggag
      541 cagtagcatt ttcaagggta ttgtctattt cgtaggcaat aaatgtatcc accttgcgag
      601 gcacatatcc tgtagcaaaa tctactaccg ataaatctac agttagtgtg ctagtgtcat
      661 ctacatccat aggtcttagt ctattgttgc ccgaacccgt ataggcagtg gctatgcttt
      721 cccacgtgcc agggtttttt accaatttaa agtttaggtt tttagagtcg ccccatttgc
      781 ctaagtcaaa attgttaatt tggtcgcggc taaaggtaat ttcgcctaca cctgcttggt
      841 taaatgttac ggcatacatc gttgctgcag ttgtattagc ttcggtgcgt ttgtcgtaca
      901 cctcgccagc tatgtagtag gtgctattgg caggaccgtt ggtaaacttg agggttagtt
      961 tgctgagttt ttcatcacta aaatcagtaa cccccaaagt ttttggttta ctggtttgag
     1021 caccttcttt tatgtccatg ccatagaatg taataccttt tctgatgtta tcgccatcaa
     1081 acatacgcaa gccaggcaat gcacgacgca tagcaattaa gccttggtaa tattctacca
     1141 ttggcacagc aatgtggcta aattcgtttg taaaacgatc ccaacgaagt tggttggtat
     1201 agtcgttaga tatatacgta ttttcgtgga aatactcaat accttcttcg gggttagtgt
     1261 aaggagtttt gtgagcacgt gttttttcaa tatcattaat tgctagtggt ttagtgcgta
     1321 aaaactcatc tcctgcatgt aaaattactt tgccttgcga agtgaatatc atggcggtag
     1381 ccaatttcat tactttggca cgttttaatc ccgatgggtc gtttaccgaa aggttaattt
     1441 tgtcccacaa ggtaaatccg tcgtgtaccg ttaggtagtt gaggcataca gcaggatttt
     1501 cggcaaaggc attgtaatcg tcggtaagta cttcggtggc aaacgcttta cctttcatcg
     1561 gaaagttttt tacgcctccc aaaatggctg taacgcaacg gtcaatttcg ccatagcttc
     1621 cttgtacaaa gcctttttgg tgttcacgtc cagcgaaagc atcgcgcgat acatcgttaa
     1681 agaagcccag gtttaggtat tcttttgggt ggtttagttt ggtggtagca gcatgtagtc
     1741 cagtagcttt ggcatcggtg tctaaatcgg agaataccca cgcttcgcct tgcaatacca
     1801 attcgttgat gttgttcggg ttgtatgttt tacccacttc ttggcgaata agacgcattg
     1861 tttcgtggtc aataaagccc attaggtcga aacggaaacc atcaaccaag tattcggtaa
     1921 caaaatgatt gagtgattct actgttaatt tttgtaccat tttgcgacgc gattctactg
     1981 taggtcctgc acccgaatgt gccgaaatac cattggtatc attgtaacgg tagtagcaac
     2041 cgggagccac attttcgaat gtttctacca aataggtatg attataaact acgtccataa
     2101 tcacaccaat acctttgctg tgtaagtctt gtaccaattc gcgtaactca cgaatacgtg
     2161 cataggggtc ggcagcgttg ctcgagaacc atccttctgg cgtaaagtag ttgtgagggt
     2221 cgtatcccca gttgtagttg cttacaggag catctgtatc agaaaactga cggttttttt
     2281 cgtctgtagt aaagaaattt tgaataggca atagttgagc atgcgtaatg cctaattctt
     2341 ttaaataatc tgttttctcg gcaaatcctt tgtacgttcc tgcaagttcg ggttttacca
     2401 ctcctggttg tacggtaaag tcacgtacat gaacctcgta acatactaag tcaacatcat
     2461 tagctatatg gtcgtagttt gtatatttac gtacaaaatt tgcgggtgtt gcacgaggcg
     2521 atttcatatc tacgatggct gctttgccta ttttgtccgt tcctttgggc gagtgtgccg
     2581 ccattgattt ggcgtatggg tcgagggcta aacgtgattt gccataagca tacacttcgt
     2641 attggtaata taatccatct aacgatttaa cgccaaggtt tttggctgtt acttgagctt
     2701 gccaaacacc atttttgccg ggcttcattt ctactttgcg tttgatgggt tgttcgtttt
     2761 ggtcgtaaaa atgaatggta attctacctg caggtggcga ccaaagttta aaagtagcta
     2821 cgttgttgtc aaggttaaca cctaaatttt gttcgttaga tgcatcaaaa taggtatcta
     2881 aaataccacc aatagcatgg tcgagaaata ccggcgattg atttcctttg atatcaatta
     2941 ggtagttggt gtttaagttg agcgatgcta ggtcgatggt aatgatgttg cctttttgtg
     3001 taaaagcagg cgtaatttta gtgcctaaac gaaagctttc tgtggtcggc tttttagcgt
     3061 aattgtaagt ataaagcgta atgtcacctt ggttgtaagg tgtggcgttt tttagggtta
     3121 cttttatctt ggtaggcgat acgataaagg ctacgtctat tacatcgttg gtagagccaa
     3181 acaaattata ctgacttaat tgtgctggta tatgcacctt gtcgatgcga tgtggattat
     3241 ttccgcagtt ttttgcaaaa gcacttatac tcatcaaaga ggaagcaata agtaggattt
     3301 ttttcatatg attacactat tgattggtta attattatct gctatttttt tatcacggta
     3361 aaaatagcaa agtttttttt atttgcaaaa aaaatacgaa tttacacacc ttttattatg
     3421 cagatagtaa tggttttaat gcgaatgtaa attagtattg atagtttgtt ggtatcatac
     3481 ctaaaacctt gtatatttgc acaatctaaa ataattggat gtttacgtta aattcggatt
     3541 tggttaagta aataagtgta ggcaattttc tatacttatc cctacaactg gtactcaggc
     3601 atattcttgt ccgagtagtt tatcataact atttattagt tggcattgaa tacgtatgta
     3661 ttttagccat atttattaac gaaacaattt taattatcat gagtaacgaa aataaatcaa
     3721 ttcacgaatt tgattatcaa ttaatttgcg actatttttc acgtatcgaa cgtcaaggac
     3781 caggtagtcc tgaagtaacc gtaaaagcct tgagttttat cgacaagcta aatgaaacct
     3841 caaaaattgc agatttaggt tgtggtacag gtgggcaatc cgcttgtttt aacggatagt
     3901 gagtgaatca aattcgttgc atcttttcac tgaaaagtaa aaaagaagtt cataaagaag
     3961 ttccaaagtg ttaccactgc gatggcaaaa actttaacta tgtaaaaatt gtagtctcgt
     4021 ttgcgaagca ccaaatatat cactccattg ttaatgccca aaccaataag agaaataatg
     4081 ataaacagca gatattgtgt agagatgttg gggtcagtgt tctcaaatgt ccacatacga
     4141 ttgagtaggt agttgctact agctgcagca ataaagccta atgaattggc aatgtattta
     4201 ttgagcttaa ttttttcttt acacagatag gtaatgccaa aatcgaccaa taagccagat
     4261 gctcctacta cacaaaattt gataaattga ataatcattg ttctattata ctatttataa
     4321 tggcaaagat aaataaaagg aattcattgt aggtgctttt ttgtgctttt ttctttttag
     4381 cttactatgt acagtttttc agggtactat ctgtatgttg tatatggggc tgttgtacca
     4441 cacaatatat ggtggtttgg taagcaaaaa gcctgtttcg tatgtttcat ttggcttggc
     4501 tttttcgctt tcaatactaa ttttttgttg gatgttggtg gttgaaaatg ctgttaaaat
     4561 aagcgggttc tctacatcaa caggaaagta ttcaacccac gaccaaggtt ttcgaataat
     4621 gtaatgaagt tgtatttttt cgctatctat cactatatca taaggatatg gattgtgtat
     4681 tgatagagtc atattctttt cttccctttt tgatgctatc gtattgtagg ggttaagcca
     4741 ctggatttgt accttgcgga taggcatata gcaactatct ataacaaaac taaattgacc
     4801 attatcagtt tgtatatgtg tagcattagg gtaatcgcca cctacgtgaa ttgctactgg
     4861 tttttctagt aagttgctat caaaatccca tagttggtat tggctggttc taaaatggat
     4921 attgggtgaa gcatgtgcat ctaaacctgt ataataactg tatttagctg ccatagtgta
     4981 tttacccata aatattactg gtaaatttcc tgtagcattg gcaatttgag tgtagcttgt
     5041 tttattatta aacacctcag ctttcatccc gaaaggatta gtggctgcaa caatgcggaa
     5101 acctatcagt ataacaatgg aaataatact aactgtacgt atatagcgat accttttttc
     5161 atggtttcta gcccattgcc aaaccaatgc aataacactg aaggttacaa aaataaacca
     5221 ttgcggttgc acgtgtccgc gtatgcatga ggcaaaaaag aataaaaaga aaaataaagc
     5281 tatgcgtttt aggattagca gtgtaagctc gtcttgatgt cggtttttga aaccaatcca
     5341 tactaccaaa ggcagaataa atggattgaa tacagcaata aaattaccta cgtattctgc
     5401 tatgtaaaag gcattaaaat cttgatttct gcctacaaga tgatactgaa acgataccca
     5461 ttcgtgctga tattgccaat ataagtgagg taacattaac aataaggata aagtggctgt
     5521 aatgtaaatt cccttgcgta aaaatacttt aggatacgct gcacaagcaa atagtactac
     5581 tagcacacct gtatatttac tataaccaat ggcggctatt gccaatgcca tgaatggaat
     5641 atagtaccaa gatgtatgtt gcagatactg cacaaagaag tataaaaata gtgctgaaaa
     5701 aagcatcaga ggtgcatcgg gaactgctac aaacccataa atatgcaata tgggcatagc
     5761 caatgcgatg atgaaaaata accaaacatc ttttttttct acggtggcag gtgctatcag
     5821 tttccatagc aaccatatat agattggttg ggatagtacc gtaagcagac gtacacctaa
     5881 ctcgccagat aaccattgcc caagataaat aaacaaggca atcataggtg gatgatcata
     5941 ataaccccaa tcgagatgct tagcaaatac tacgtaatat gcttcatcat tggctagttc
     6001 tgttacactg gcttgcaaca gatttaacaa catccatgct atgttcaaaa tccaaaatag
     6061 ttgattggct gacagtttat tcttattcat agtaaggggt tattttacaa tactcacact
     6121 atctactttt tctccttgta aggtgtatga atataaagtt aagatgttat tgtcaactgt
     6181 tatatgctga aataaggctg tgtttggaat aattgcatcg gtaatttttg ttgctttaaa
     6241 gctgggataa aacttctttg acagatgaga ggttacgtaa ataggtagtt tactagtgcc
     6301 gcttttgtct ttttttgaat tacgagcata tccatgttcg tgtccttgta gtaccaaatc
     6361 aacaggatat tgtgataaaa taggttccat catcaaacgg atagatgcat tgttccagct
     6421 acttttgttt gataatacag ggtggtgtag cattaccact gtaaatagag atgagttgtt
     6481 ttttaaaact tcttttaggt aatttcgttg ttgccaaatg taccaaggtt cacgatttga
     6541 gtcgagcaag ataaaccgca cattaccata tacaaaagta aatacatggt tgttgttatg
     6601 gttattgata aaataaggga atgtataagt aaacctatca tcaattttgc ggatggcata
     6661 tttaaggtat tcgtgattgc cagcaatagc tataattgga gtagattgcc ttatgccatc
     6721 aaggtcgttc ataaaagcct cccaatagct attcattgga cgctcaattt ggtcgccacc
     6781 aaaagcccaa aaatcagcct caggaaaatg ttgatatatg tattgaaaag cctgtttgct
     6841 ggtattactg gtatctctgt cttgcacatc gcctatgtac ataaaagaga acggactatt
     6901 agttgatgtt gttttaaact ggtaccattc agtttgtttt tcgcccgaaa gtacttgata
     6961 tgcgtattgt gcattacttg ataaatcgtt caattgggca gtataaaagg ctgttactcc
     7021 tccacgcgag cgtacaatgt ttcctttggc agtaattaca agcgtgtcag tagaatgtaa
     7081 tttggttagt tttaccgttg cttgttgtat aacagagtcg tgttgccagc taagtatccg
     7141 ttggttggtg ttttccccca aggtaagtaa gattctgtgt gggtagggct tacttgtaaa
     7201 aggcatttcg gggcgattgc caaaccacac attccatcgt tgccaaacca tacctccaat
     7261 tgttataagc aacagactta ttacaagtag gaggtatttt ctttttttat tcattggcaa
     7321 gggtatagtg tataaaacaa tagtttacat ggtaagctat tgcaacaaac tttttacttt
     7381 ttcagaaata agttttcctt ctacttttcc gcttaattgt ttggtggcta tgcccattac
     7441 tttgcccata tctttagctg aggtggcacc tacctgcgag ataatggttt gaatagcagc
     7501 agtaagttct tcgtcagata attgcttggg taaatagcga gcaataattt ctgcttcttc
     7561 aagttcttta tcggctaact ccttgcggtt ttgttcggta tagatttgag cagattcttt
     7621 gcgttgtttt accatttttt gaagaatttt tgtacccatt tcttcgctta cagtgccgtc
     7681 gccacctttg gctgttttgg cttctaacaa ttctttttta atgccacgta gagcgtcaag
     7741 aacggttttt tctttagcca acatggcttt tttaatatct tcggttattt ggtctattag
     7801 ttccataatt agtcaacatt atcgtgtaaa aatttatttt tttgagagaa taatacgtca
     7861 tcatccgttt tttctataaa aattgacgaa cgttcactag ccgatggttg agtatttggg
     7921 gtatctatat tattacgctt aaatgctgga actgattcaa actgttgcat tttagcatcg
     7981 tcatcccatt cgtccatatc aatagtttct gttacaacag gtttgggggt tggtttagag
     8041 taaaaatcat ctttggatag aggtatacct gcaattggag attgtgtttg agtagctgtt
     8101 gtattgctac ttgtattttc agaaatagta ccatagtcaa gttctactgt atttgtttct
     8161 tttattacag gtacgttcag tttatccatg ccaaatccag tggcaataat ggttattttt
     8221 attgattctt ccagattttc atcgtatgta gcaccccaaa taacttctgc atctttagca
     8281 atattgttta agaaatgttt aatctcaatg gtttcagtca tgtcgagttc attttctttg
     8341 gaggtgtata catgtaatag tatgcgtttg gctcctttta tatcgttatt attgagaaga
     8401 ggagagttca atgcatcgtg aatagcttta cttacacgtt tttcgccact agcaaaccct
     8461 gtattcataa tagcaaaacc accttctttt aaaatagttt gtacatcagc aaagtccaca
     8521 ttaatatatc ctggaatggt aattatctcg gcaattccct tggctgcatc tgttaggata
     8581 ttatcagctt tggcaaaggc tgttgacatt tttaaatctg gaaaaatatc aataagacgt
     8641 tcgttattaa tcactaacag agcatctaca tgtttgctca tttcgcgaac cccattcagt
     8701 gctttctgaa tctttttttc tccttcaaat tcaaaaggga ttgtaacaat tcctactgtt
     8761 aagatagatt tgtttttggc tatttctgcg ataacagggg cagcaccagt acctgttcct
     8821 cctcccattc ctgccgtaat aaataccatt ttagtactat catccaatag ttcttcgatt
     8881 ttgtccatgg tttcaagggc tgcttcgcga gctacctctg gtatattacc cgctcccagt
     8941 cctttgtctc ctagttgtat tttaataggg attggagagc gtcttaaatc ttggtaatcg
     9001 gtattacata cagcaaatgt aacgtcctta ataccttgat taaacatatg atttacagca
     9061 ttgccaccac ctccacctac accaattact tttataattg tatcagcatt tgttgaaaaa
     9121 tcaaaattta ccatgatagt tatttttagt ttgttgtctt attgaattgt atcactattg
     9181 tcggtaaata aatctgttaa tcgttgtgat gcactatcgg caaaacgtac gaataaattt
     9241 ttcccagttt tttttgtttt tttcgatggt ataaattttt cttcaatagt ttctgatacg
     9301 caattttctg ttcccattga taacaagcca ataatttgac tgtaagatgg ttttttaaaa
     9361 tcatccgaaa tagaatcttc tgtaagtaaa tgtacaccaa cagtagtacg aacgtccaca
     9421 ccactcagcg attgtaccaa agctctcata ttcttgagtt gagctcctcc tccagtaata
     9481 atgataccgg caccgagttg ttttatgtat cctttttgtt ttaatggggt aatgatgaaa
     9541 tttaaaattt cacttgccct tgcttcgata atttgagcta aaaatgtgct ttgtatcgat
     9601 ttttcttctt gttcgtgagc tgctttagcg ataataatac gcttatcagg cacctctaaa
     9661 gttgccattg cattgcctag tttttgcttt agtttctcgg cttgaggttc cgttaaatta
     9721 agttctctta tatcgtttgt aattgtttta gctcccaatg gaatgacacc tatttctctg
     9781 aggtaatttt ttgtaaatac actatacgaa gttgtactcc ctccaaaatc aattatcata
     9841 cagccaagtt ctttttcgtc atccgaaata agtccgtttg ctgtagcaat gggagaggaa
     9901 aatgtgctaa ttatttctaa ttgtgatttt tctgccaaga gttcttttac tttgttgatt
     9961 cctgtattaa tttcgctttt tgtataaata attttgtaac gtccttctaa ttgtgtgcat
    10021 gctatacctg caggatctgc tacccactca tcatcaacta aatattcctg agtgattaca
    10081 tctaagatat caaactcagt agattgtagt ttaaagttct cttgatacaa tttatcaagc
    10141 aaattttgtt ctacaatttc gttataacct aatgtacgtt ttgttgtgtt ttctttgcaa
    10201 cgcaaatacc tgccacctat accaatatat gctttggtta ggtttaactt agttttgtta
    10261 cgtaattttg taacaatact attgataata aaagctgtgt ctgccacatt aaatatagcc
    10321 ccttgtttaa tggtagttgg cttggtttcc tcttcttcgt atgccacaat tgagagcttt
    10381 ccttcggttg ttttttgagc tactgcacca attattttgg ttgtacccaa atcaaaagct
    10441 aggatgtaag gagtagatgt gttcatagta ttggaattat tttttagttg catatagtaa
    10501 tttcttgtat tcaatattga tatttgagta agtgtctgtt aaagcaaatt caggatatgt
    10561 tttataaaat gcatatagtt tttttaattt ggtttcataa tcatcaagtg tccctaatgt
    10621 aatagtgtat aatcctactt ttggcactaa ttcaattttt ttattttcct tgatgtatat
    10681 ttgatcaatt tgagcattcc aaaatgaatc gttggtgata tatgttacaa aatcaaagag
    10741 ttcgtttttg gcaaaacttt ctgttacatt accagatacg ataggcaata taaccgtgaa
    10801 atctttagat attggcatct tttttctgtc ttgatcaatg taataagaat agaatggtgt
    10861 aataatacga acaataggtt ggcgttgcca tatgcgtatg accacatctc catgattggt
    10921 tcgataacac tctacccgtt ttacatagtt catctctttg attttttctt caatagcatg
    10981 tgtaaatgcg aaatcaattt ttcgtcctat cggatatata ttgttatctg ttaattgttt
    11041 ttcaatttct ttgcgtttaa taaaattata atcaacacta tcaattttga aaatatgtat
    11101 tgtactacac acttgctctt gtggtgtatg ggtaaaggag attgctgcgt aaattaaata
    11161 cgacagaaac actaccgaaa ctactataag agcaatcttt ttaatcattt ttatatcaat
    11221 taaagctgtt ttttaagttg ttgagtaatt agtggcacca ttttatctat atctccagct
    11281 cccaatgtaa ttaagacgtc aaaattatac ttttttatct cctctagcaa atcttctttg
    11341 ctacaaattg acttattttt gcattgtatg tgtttgaata tcatttttga acttactcct
    11401 ttgataggta gctcacgagc aggataaata tccaataaaa taacactgtc aaggagcgaa
    11461 aggctattcg caaattcagt tgcaaaatca cgtgttctgg tatacaaatg cggttgaaaa
    11521 ataccacaca ttgacttatt tggatacagt ttacgtattg attgtataga ggcttttaat
    11581 tcttcgggat gatgggcata atcgtcaata aaacgtacat tttctgtttg taattgaaaa
    11641 tcaaagcgcc tttctattcc tttaaatgtt tcaatagcat gttgtaattc ttctttagta
    11701 acaccgttta gatatgctat ggtcatagca gctactgcat tttctacgtt gattaaaata
    11761 ggaactccta gtttgatttt ttctattgta aaagtaggcg taacgtaatc aaaaagaatt
    11821 ttctcatttt caattagaat gtttgtagca taaaaatctg ctacttcatt tgctgaatag
    11881 gtgtatatct ttaccgaatt ctttgtgcta ggcactaact caataccttt tttaagcaac
    11941 aagcatccat tttcttgtat aagtgatgta aagtgttgaa aactagatag taggttttct
    12001 ctatcttgat atatatctaa atggtcatta tcggtggctg taataatagc catatgtgga
    12061 gataactgat gaaatgatct gtcatactca tctgcttcca ctactacaat atctgatgag
    12121 gcattcacca ataaatttgt gctgtaattt tttgaaatac ctcctaaaaa agcattggtt
    12181 ccgaggcttg attgtgtgag aatatgggca aggatagttg aagtagtcgt ttttccgtgt
    12241 gttccagcta cacataaggc tttttttgaa cgtgtaattt cacctaatat ttctgctctt
    12301 tttagcattg tgaaaccagc tttttcaaag aaaactcgtt cgctcatgtc gtgtggaatt
    12361 gcaggtgtat atacgatagt cgtattttcc ttatccatat atttcacagg aatggtattt
    12421 acactatcgt tgtaatgaat ttccatccct tctgactcta atttttgtgt aagtgtattt
    12481 tcagtagcat cgtaccctcc tacctgatgc ccaagttgtt tgaaatagcg agctaaagca
    12541 ctcataccaa tacccccaat acctaaaaaa taataatttt gttggcacga ttttattttt
    12601 ttagtcgttg attttccctt tttttgtatg ttatgtgata catcaatacc aattattttt
    12661 aagatttctg tagcaatgtg attagctgct tctggtttag caaaatgttc aatatttttg
    12721 ctaagatatg tcagtttggt atcgttgcta atgagtgtta gtgctgttga aataagaaac
    12781 tctggtgctt cttggtcgct tatcataaca gcagcttgtt tttcgaccaa cgccattgca
    12841 ttttttgttt gatgatcttc agaaacattg ggcgatggca ccaagataca tggtttttgt
    12901 agcatactta attctgatat agagcttgct cctgcacgcg aaataaccaa gtcggctata
    12961 ctataagcca agtccatacg agccacaaaa tcagtaataa cgatgctttt tgaatcaata
    13021 tggcttgttt gttgttttat actattgaaa taatacttcc ctgtttgcca tataacttgt
    13081 atgccagtat ctgctagctg attaatatgt tgcaatacgc tttggtttat agtgcgagct
    13141 cccaagctac cacccacaat gagaagggtt tttttagtag ggagaaagcc aaactcgcaa
    13201 tacgcttctt ctttgttttg actattttct aataattgtt gacgtacagg atttcctgta
    13261 agtgtaattc tatctgatgg aaaaaatcga tgcatattat cataagctac acatatgcgt
    13321 gaagcttttt tggcaagcaa tttattggta actcctgcat acgagttttg ctcttgaata
    13381 actgttggaa tacctaattt atttgccata tacaatgtag gtccactagc ataaccccct
    13441 acaccaacta caatgtcggg gttaaacgat ttgattgttt tttttgcttt atataaactt
    13501 ttaatcaaat tgggaaaaaa tgctatatta cgtttcatat ccgaacggtc aaatccgctt
    13561 actggtaacc caacaatagg atatccagct gcaggaactt tctccatttc cattttattc
    13621 tcagcaccta caaataggat gtctgcttct ggcaatactt cttttaggct attggcaata
    13681 gaaatggcag gaaaaatgtg tcctcctgtt cctcctccac taataatgat acgtacgtgt
    13741 tctttcatag atgtatgcta tttaatatcg gtttgtcttg tttctgtgtc tgatgctttt
    13801 tcagccgtat cttcactgtc tgacacaact tcattttgtg cttcgtcagc aggagcattc
    13861 gtagtattgt tggatacttt ttcttctttt ttgatatatt gactaatatt taatatgata
    13921 cctaaataca cactagtcat aataatagat gtgccacctc gactaatgag cggtagtggt
    13981 tgtccagtaa ctggacctaa atgagacgcc acagctatgc ttataaaagc ttgaattaca
    14041 atcaataaag atagccctac aaccataaat gtagggtagg ctttgtcgca ttgggtagca
    14101 attcttccag atcggaataa aattattagg tacagaagca taataaatat ccctccagct
    14161 aaaccatatt cttctatcac aatagcataa ataaaatcag agaaagccaa tggtagaaaa
    14221 tctctttgtg tactatttcc cggacctgca ggtgtcatcc cgtgtgcaat tgcaatttta
    14281 gagtgcatga tttgcatgtt atcatctgtg ataacatatg tcttttccgc agaaggttca
    14341 tttatttcgc taacaaaatc gtctatacga cctacccacg tatttaaacg acctaagaat
    14401 tgtggctgaa agtaatgtga aataacataa agagaaagta ataaggctgc taacactcca
    14461 atagtgatac ctaatttggc aaaggaaatt ctacctatca tcattaatag aatacctaca
    14521 ccgaataaca ttacggctgt agaaaggttt tgtggggcaa ttaacaaaca gaatatgcct
    14581 aaaactgaaa tgatgggcca aaaagcttta tcggcagttt gaccttcttt ttgatttctt
    14641 gcaagcatat cactgataaa tacaatggta ctgaattttg ctaattctag tggctgaaac
    14701 tcaaatccta atactttaat tgcacgtatt gcattatttg cttctactcc tataaatggt
    14761 aataatacta gcaaaactaa tgcaatgggg agtatcaaat agtttagtcc tctgatatag
    14821 cgatacggaa aacgatgtac aataatcatg gctattaagc ctaatccgag catttgaata
    14881 tggctaaaaa atggagcaag atgattactg cgtctaaaag cctcttga
//
LOCUS       DAOSRP010000035        14808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4133_length_14808_cov_4.929641, whole genome shotgun sequence.
ACCESSION   DAOSRP010000035 DAOSRP010000000
VERSION     DAOSRP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14808
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_14808_cov_4.929641"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLJ82_03500"
     CDS             complement(<1..359)
                     /locus_tag="PLJ82_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40603.1"
                     /translation="MKRKTVKIYGCVACMLFVCINLYAQVAIPAQVKQDSIGSIQVLA
                     TTMQKTIALRWSASNANTWQQTNETGFLIERLTIKRNGVALKEPETILLTNSPLKHAP
                     IQVWATKADNDTTAMIVA"
     gene            848..3838
                     /locus_tag="PLJ82_03505"
     CDS             848..3838
                     /locus_tag="PLJ82_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOR40604.1"
                     /translation="MKKLFTPSFFSYSLFVLVFLAPSAIYAQKTEIDTICSGSPITLT
                     ATTGDSYLWSPGGETTSSVTVTPTETTKYTCDVTTNGTKINTGNLILGGDFEFAIPTG
                     FSMGSQLKLEGSITDPITNIKSNLYTQMNNFGSGMTSETAGILNVGGAMTTTDPYLLK
                     DNPSDTTWASTCGRSYFIHLKDHTKADGTGKMLAVDGSSGGEIWRSGNMQIKPGKTYQ
                     FSCWVANIDAQFGTSESKTDPITGKTILTSHDINTLAKLTFKIETGKGTSTFPTFTVS
                     QTVGEWTEYTGKYTVPGTGTSTYCNISLTNATGIPEGNDFAIDDIYFGLEQNTGPVTI
                     KDNFTVNVDTPATVTMASINVCQNAGFTLSATVSGGTNNVISWTKNENTLTGSTAAYI
                     SNTAPATVGESDTYAISVKSGVCTAVTQSATVTALSCNSTQKDTICLGNNIVLNQANT
                     KTYSGISWSPATGLSSTNILKPTFTPATAGTFAFTMTGTNTATSLIETIYVTVKVETP
                     ATVSVDGVSQCINNPITLKAKVSGGTNNSIAWTKNGNTLSETTNEVSDTAPATINETD
                     TYRITVTPTGVCPAVSSEATVTGTSCGVQAIINQQDIDICPGESLQLKALSSTTAHAW
                     STGESTQSVQVSPTTKTQYVVVSGPSIPSDVIDFLENNVSPVNVSEISDILGYQTEQY
                     YIDIFNVTIKDCGVTTILNRIVCQGETVTVGTQTFSTSGQHSYTLVGGSYLGGDSTVI
                     LNLRVIDVAIQAPKTAICMGESLSLGATANPNTEWSATWTTQPSTGLSLSNASDTTTN
                     ASGTIAGNYTVTLTASITQSDGIYAITPHTCTATQAIVVHPNPVITDIFTTGRAATIL
                     YDQQSGTAPFTFNIGNTVYQHNELFDLPIGEYRVEIQNAEGCKSFRMFTIVPTPIIPD
                     KFFSPNNDGVNDRWNVVGIEFYPNADIQIFDRFGKQMIRYKGSDLGWDGNYLGNPMPT
                     TDYWYIIYVAEINETLSGHFTLKR"
     gene            complement(3886..4083)
                     /gene="xseB"
                     /locus_tag="PLJ82_03510"
     CDS             complement(3886..4083)
                     /gene="xseB"
                     /locus_tag="PLJ82_03510"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOR40605.1"
                     /translation="MKKEKKLTYDEAYAKLEAILHKIESNELGVEELLENIKEATTLI
                     DYCKNRLKTIDQEVAKLIEGK"
     gene            complement(4340..4711)
                     /locus_tag="PLJ82_03515"
     CDS             complement(4340..4711)
                     /locus_tag="PLJ82_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOR40606.1"
                     /translation="MEFDSITKEIIGSAIEVHRQLGPGLLESAYEECLVYELQQRGYK
                     VERQKPVPVVYKDIKLDYGYRIDILVEDKILLELKSVDALAPVHEAQLLTYMKFSAIK
                     IGLLINFNVTVLKNGIKRYVL"
     assembly_gap    4872..4971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5202..6452)
                     /gene="xseA"
                     /locus_tag="PLJ82_03520"
     CDS             complement(5202..6452)
                     /gene="xseA"
                     /locus_tag="PLJ82_03520"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOR40607.1"
                     /translation="MPPHAHIRLVELNSFVKKLIADNTQPQWITAEISEVNEHYSGHC
                     YLELIEKDDADEHIIAKAKAVIWSFTYRMIKPYFETTTHERLMAGMKVLVKVEVSFHE
                     AYGYSLVIKDIDPQYTLGDMARKRALIIQQLYAEGVMDLNKEIDLPLLVQRIAVISSP
                     GAAGYTDFCNQLHGNAFGFVFYHRLFAAVMQGTETEASIINALEAVYEHKELFDVVVI
                     IRGGGATSDLSWFDNYNIAYHCTQFPLPILSGIGHDKDVSVVDMVAHTSLKTPTAVAQ
                     FLVDCMQQTESNLHTVKEEIINYTEQALESWQQRLQLVTLQLPNAVKRKVEQAYYTLT
                     SSIQHLQHRSKQNLTQKAYTLDKHQQYVQLVSPIRIFEKGYTLTLKNGKPIHNQTLAK
                     GDVVETISLQKRMKSTIIEIENGK"
     gene            complement(6468..7229)
                     /locus_tag="PLJ82_03525"
     CDS             complement(6468..7229)
                     /locus_tag="PLJ82_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOR40608.1"
                     /translation="MKQFLCLSAFFLFSISLFTQQAVFKNRYYPNGNLMYEGYFVEDK
                     PVGTIKRYHENGKLKAIQQFATNDTVKVEYFAGDGSQLAKGIFVKGQKQGIWEYFSKE
                     NRLVMVENYTEGLKQGESLVFSESGMVIDRMQYENNVLHGKRIQYHPYGNILASYFYK
                     NGLLDGPFESYYETGQKDEVGYYVANKKHGEWKTYNDDGSFTSVEYKNGKATNQTELD
                     KKLQETMDKNEQGDPTIKDPADYLDQPDAYLNLFR"
     gene            7531..9663
                     /locus_tag="PLJ82_03530"
     CDS             7531..9663
                     /locus_tag="PLJ82_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOR40609.1"
                     /translation="MKKNVPFFLSSIKHQAVTSRLRQLFLGIALFCIVPLWAAVQINS
                     VNTSHVSCNGLSDGSITIVATGGSGVFQYSLDNFVNSIQSNSTFINLPATNTNYSVYV
                     RDANNHTDNTWQTVSITQPLPLTFNPTKDFIQSVSCHGASTGKIKIEAEGGNAPYLYI
                     INSLDGSISYSNTVGVFDNLPATTYFVQVKDANGCITNHADMVVPQAELLEAELMSKT
                     KIVCKGDMTASVRFKIKGRTEACGLLKADTTAYYSAKLYNITTGVDVLEGEFTVTNQL
                     HPVLTQNIQIPVLDEFGNPVVTETGNPVMQTVKDTLYAEGCHEPTKMGVVPAYTVDKK
                     GFDCDDYISIGKLGEYAYRLELYRRNCQIGTKFEFVIERSGDIPTLSLNGVNPICDGS
                     SEVIKATIVATPATTTYKWILNETTIATTKDLTRVFTMDDNQGKLKFEAKNTCGTVVS
                     NEIALSVLPRPTGIVTTEKGYLCQGQSSNVTAFLAGKAPFTYTLTGNTEQTTSKVSET
                     VSMSPTSTIDITFESLSDANCVATPADITGTSITVYPAPSIDITVNVPSPMVSGRYVT
                     ATVTPGFVDYSLWINNQEIGALATEENVFKSKNFVYGLSSNIFDTKVEDTNGCIWDFS
                     KTEDITVVLFPNVFTPNNDGVNDVFLEGYNLKVFDRLGTVLYSGTKGWNGIYNGVPVN
                     KGVYLYTVDILDENGDTVIIKNTVVVEK"
     gene            9690..11879
                     /locus_tag="PLJ82_03535"
     CDS             9690..11879
                     /locus_tag="PLJ82_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR40610.1"
                     /translation="MKITKKSFLFALLVCLPIISMMGNMKVGNNKYERRDYLGAIEQY
                     KKTAEGNSNEKTVANAKYKIGECYSLLNEPKEAVSYLGEAIAGGVPTLEAYVLYGKNL
                     LKMGEYARAKASFEEFDRLSPKDARIKNLIASCDFALKHSSKNPITPEQDLKGINTSE
                     IEYGIGFYQDGIVYASVEQAKKGLSSRMYFSHGKDNFTSSRLVDMIKIRTGQNVGTFA
                     IDTLDNILYYTRCNNNERDNCYIYTARYKKGKWKEKGALNIGNRHVNVAHPALSPDRQ
                     RLYFTANLEGGFGGTDIWYVTRNENGKWNKTPINVGAVVNTAGNEAFPYVVGNTLIFA
                     SDGHIGFGGYDLYSAQIDGKAVHKVQNLMKPINSSADDINLIVSEEKNEALLVSSRNI
                     STKDDIFRFEGIFTSMMVSGHAYNNVTKEVLPNTQVVLSGMGKTHTTETNKDGYYYAF
                     IEAGDLYKMFANAVGFLSDMAMIKAEKTTIGSFPVEQDFYLTPASMSISGRVYDMETN
                     EPFINEDVLLLENGTLAQQTKTDITGRYSFTNLTNGKEYQVKVNKPNFLSITSKPFIY
                     QEGDANNSSFDLASIPYGSDSFDPSGVGGVGGMGNTSNVGGINGGRFAGREISINEIY
                     YDFDNANLLPQSRFSLDKIILLLSSNPTLKLEFGSHTDTRGSAEYNLQLSNARAKSVV
                     DYLIASGISRNRLSWKGYGKTNPIVKNAVTEEEHRLNRRTTFKIIER"
     gene            11897..12823
                     /locus_tag="PLJ82_03540"
     CDS             11897..12823
                     /locus_tag="PLJ82_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOR40611.1"
                     /translation="MKKILIACCASLLFSLSVFAQHDAQISQHLLSKTHYNPAAAGAS
                     LYGNITGIARQQYVGFTGAPQTGLLNFDMLLPNTSSGIGLSVVYDKYGPLLSYNPMMN
                     YAYHIKLGDNQHLSLGVAAGVFLRQYDASNNIYENTGDPLENYDGAQEYLAPDANIGI
                     EYQLENWIIGASATHIPQFFEEETVMNPTTQYYAYSRYRFEIGRYWDLTPGIAAQYDG
                     YSVHSEINAVLHYLKLFWVGGSYRFSDVFDSESIVPMIGLQLSDYVRLGYAYDYNLSS
                     LQNYNQGSHEIMLQLRFKTRHHTSRTPRFAEW"
     gene            12958..>14808
                     /locus_tag="PLJ82_03545"
     CDS             12958..>14808
                     /locus_tag="PLJ82_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOR40612.1"
                     /translation="MADILDTKEQAENELIEQEYQLLIKEYLASNHRKKVARIEKAFL
                     LAKAAHKGIKRKSGEPYILHPLAVARIVAKEIGLGSTSICAALLHDVVEDTEYTVEDI
                     ANLFDPKIAQIVDGLTKISGGVFGENVSGQAENFRKLLLTISEDIRVVLIKIADRLHN
                     MRTLGSMPAAKQYKIAGETEYIYAPLAHRLGLFAIKTELEDLSFKYNFPSVYQEISEK
                     INENQLERMLLYDKFSLPIINKLNSLGYKYSIRARIKSVYSVWNKMKKKNLPFEEIYD
                     ILAVRIVFEPNQNVDEKEQCWIIYNNITNLYIPHPDRLRNWLNTPKANGYEALHVTVM
                     GPDGQWIEVQIRSARMDDIAEKGLAAHWKYKQQFYGDSELDKWLNTINDILKNPSPNA
                     MDFLDTFKLTLYATEIFVFTPKGEIKTLPSGSTALDFAFEIHSDIGMRCIGAKVNNKL
                     VPLSYELQSGDQIEILTSKQQTPQPEWLSYVTTARAKQKLNLLFKNEQKIVKTEGETI
                     LRKALTEAGFVIKKDISNDIIKKLLHHFEINSFEELTFQIGKGSISLANIQDIVKQEN
                     KKTTVRSWYPRFFFGKDTDKENTATASARIKKNGTYQLTDEMLQKEHTIAP"
BASE COUNT         4795 a         2793 c         2668 g         4452 t
ORIGIN      
        1 gcaacaatca tagctgttgt atcattatca gcttttgttg cccatacctg tatgggtgca
       61 tgttttaagg gactattggt aagcaatata gtttcaggct ctttcaacgc aacaccattt
      121 cttttaatag ttaatctttc tatcaaaaaa cctgtttcat tggtttgttg ccatgtgttt
      181 gcattactag ccgaccatct tagtgcaatc gtcttttgca tggtggtagc aagcacttgt
      241 atggatccga tagagtcttg tttgacttgt gctggaatag caacttgagc atacagattg
      301 atgcatacga ataacatgca tgcaacacat ccgtagattt ttactgtttt tcttttcata
      361 gtatctctta tcttcattca ttataccttc aacatcagct acatgagtat gattgtgtgt
      421 acggacatac catcacacac aacaacaagc atattgcctt attttcgctc gcaaaagtac
      481 agttattttt tcatttcaca agcatatgta tcatcactat ctatagaaat aaccacattt
      541 tgttaatgat aaaatcattc gtacagatac aatatactac gaaaaggaca taactgagga
      601 tatcatttag actttttttt tagaatataa tcgcgatgca tttcaccaat tacctatata
      661 actttgctac atacaatcga gcgataattc gtgtttatac agcataaacc ataggttttt
      721 ttgaggcgtt atgtttgata cgtaacaaga taacattgtt aaaaacaaaa aaataaagtg
      781 tataacattt tttaatatca catttttctt ttatatttgt ccacctaaaa cctgaaacta
      841 tctgactatg aaaaagttat tcacaccttc cttttttagt tatagcttat ttgtattggt
      901 tttcctagct ccatctgcta tatacgctca aaaaaccgaa atagacacaa tctgttcagg
      961 aagccccatt accctaaccg caaccacggg agattcttat ctgtggtctc cgggcggtga
     1021 aacaacttcc tctgtaacgg taactcctac tgaaacaaca aagtacactt gtgatgttac
     1081 tacaaatggg acgaaaatta acactgggaa cttaatactc gggggagatt ttgagtttgc
     1141 aattcctaca ggatttagta tgggttctca attaaaattg gaaggtagca ttactgaccc
     1201 cattacaaat ataaagtcaa atttatacac acaaatgaac aatttcggga gtggaatgac
     1261 tagtgaaacg gcaggaattc ttaatgttgg cggagctatg acaactactg acccttattt
     1321 actaaaagat aatccttctg atacaacttg ggctagtact tgcggaaggt catattttat
     1381 tcatttgaaa gaccacacaa aagctgatgg aacaggaaag atgttagctg ttgatggaag
     1441 cagcggtggc gaaatatggc gatcgggtaa tatgcaaatt aagcccggga aaacctatca
     1501 atttagctgt tgggttgcaa atattgatgc tcaatttgga actagtgaat caaaaactga
     1561 ccctattaca ggaaaaacta tcctaacatc gcatgatata aacacgttag ctaaattaac
     1621 ctttaaaatt gagacaggga aaggaacttc tacttttccg acctttacgg tttcccaaac
     1681 agtaggagaa tggactgaat acacaggaaa atatactgtt cctggcacag gaacttctac
     1741 ttattgtaat atttcgttaa caaatgctac aggaatccct gaaggcaatg actttgctat
     1801 tgatgatatt tatttcggct tagaacagaa tactggcccc gttactatta aggataattt
     1861 tacagtaaat gttgatacgc ctgccacagt aacaatggca agtatcaatg tctgtcaaaa
     1921 tgctggtttt actttgtctg ctactgtaag tggtggcacc aacaatgtaa tttcgtggac
     1981 aaaaaatgaa aataccctaa caggttcaac agctgcttat atttccaata cagctccagc
     2041 tacagtaggt gaaagcgata cgtatgctat cagtgtaaaa tcgggtgttt gtacagcagt
     2101 aacacaaagt gccaccgtta cggctttatc gtgtaatagt acccaaaagg acactatctg
     2161 cctaggaaac aatatagttc tcaatcaagc aaatacaaaa acgtacagcg ggatctcgtg
     2221 gagtcctgct actggattaa gcagtaccaa tatcttaaaa cctacattta cacctgcaac
     2281 tgctggcaca tttgcattta caatgacagg gacaaatacc gccacaagct taatagaaac
     2341 gatatatgta accgtaaaag tagaaacacc agctacagta agcgtggatg gcgtatctca
     2401 atgtatcaac aaccctatta ccttaaaggc taaagtaagt ggaggtacca ataatagcat
     2461 tgcgtggaca aaaaatggaa ataccttgtc agaaacaacc aacgaagtat cagatactgc
     2521 tcctgctact atcaacgaaa cagatacgta tcgcataaca gttaccccca caggcgtttg
     2581 tcctgcggta tcgagtgaag ctacggttac aggcacttcg tgtggtgtac aagctataat
     2641 aaatcaacaa gatatagata tttgtccagg tgaatcgctt caactaaaag ctctttcatc
     2701 tactacggct catgcttgga gtacaggcga atctactcaa agtgtacaag tatcacctac
     2761 taccaaaaca caatatgtgg tggtaagcgg tccgtcaatt cctagtgatg tgattgattt
     2821 tttagaaaat aatgtgtcgc cagttaatgt ttcagaaata tcagacatat tgggctatca
     2881 aacagaacaa tactacatcg atatttttaa tgtaacgatt aaagattgtg gcgtaaccac
     2941 gattctaaac cgaattgttt gtcagggaga aacagtaact gttggtaccc aaacatttag
     3001 caccagcggt cagcattcat acacgcttgt gggtggtagt tacttgggtg gcgatagtac
     3061 ggtaatactt aatttgcgtg taatagatgt agcaatacaa gcccctaaaa cagctatctg
     3121 tatgggtgaa tcactcagtc taggagcaac tgcaaacccc aatactgaat ggtcggctac
     3181 ttggaccact cagccatcaa cgggtttgtc attaagcaat gcaagtgata caacaaccaa
     3241 tgcatctgga acaatagcag gcaattatac agttactttg acggcatcga taactcaaag
     3301 tgatggaatt tatgccatta cgcctcatac atgtacggct actcaagcca tcgtggtgca
     3361 tccaaaccct gttatcacag atatatttac cacaggacgt gcagccacaa ttttgtacga
     3421 ccagcaaagt ggtacagctc cttttacttt taacattgga aatacagtat atcaacacaa
     3481 cgagctgttt gatttgccca ttggcgaata tagggtagaa attcaaaatg cagagggatg
     3541 taaatcgttc cgtatgttta caattgtacc tacgcccatt attcctgata aattcttctc
     3601 acccaataat gatggggtaa atgataggtg gaatgtggtg ggtatagaat tttatcccaa
     3661 tgctgatatc cagatatttg atagatttgg caaacaaatg attcgctaca aaggatctga
     3721 tttgggatgg gacggcaatt atcttggtaa tccaatgcca actaccgact attggtatat
     3781 tatttatgta gcagaaatta acgaaaccct ctcgggacat tttaccttga aaagataaat
     3841 cttgttgagt gtgtatggag aatttgatac acactcaatt ttttactact tgccttcaat
     3901 taatttagcc acttcttggt ctattgtttt gaggcggttt ttacagtaat caatcagtgt
     3961 agtagcttct ttgatatttt ctagtaattc ttctactcct aattcattgc tttcgatttt
     4021 atgcaaaata gcttctagtt ttgcgtaagc ttcatcgtag gttaattttt tttctttttt
     4081 cattttttat atttttaaag agattatttc acagaaggac acagagtaat atattttctt
     4141 ctcagtgtat cttcgtgttt tcttcaagtt ctgcgtgtaa gaatctttca cagagttcca
     4201 cagaggtttc acagagagac acagagtaat gtgtttttct tcgtgaaact tcgtgattcc
     4261 ttcgtgctct tcgtgtgaga atcttacaca gagttccaca gaggtatcac agagggacac
     4321 ggagaatagt acttattttt tataaaacat atcgtttaat tccatttttt aatactgtaa
     4381 cattaaagtt aattaataat ccaattttaa tagctgaaaa tttcatataa gttaataact
     4441 gagcctcgtg tacaggagct agtgcatcaa cagatttaag ttctagtaat attttatctt
     4501 caactaaaat atcaattcta tatccataat ccaattttat atctttatat acaacaggaa
     4561 caggtttttg tcgttcaact ttatatcctc tttgttgtaa ttcatatacc agacattctt
     4621 cataagcaga ttctaataat ccaggtccaa gctgcctatg aacttctatt gctgacccaa
     4681 tgatttcttt cgtgatacta tcaaattcca tttttatatt ttattctgtt tttgcgtttg
     4741 agattcttac acagagttcc acagaggttt cacagaggga cacagagtaa tgtgtttttc
     4801 ttcgtgaaac ttcgtgattc cttcgtgctc ttcgtgtgag aatcttacac agagttccac
     4861 agaggtttca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgtgtttt
     4981 cttcgtgctc ttcgtgtaag aatcttacac agaggtatca cagaggtatc acagagggac
     5041 acggtgcaat gtgtttttct tcgtgaaact tcgtgattcc ttcgtgctct tcgtgtgaga
     5101 atcttacaca gagttccaca gaggtttcac agagagacac agagtaatgt gtttttcttc
     5161 gtgaaacttc gtgattcctt cgtgctcttc gtgtgagaat cttacttgcc attttctatt
     5221 tctataatag tacttttcat tcgtttttgt aaagaaattg tttcaaccac atctcctttt
     5281 gccagtgttt gattgtgaat tggtttgcca ttctttagtg taagcgtata tcctttttca
     5341 aatatgcgta taggcgaaac caattgtaca tattgctggt gtttatctaa ggtgtatgct
     5401 ttttgcgtaa gattctgttt actacgatgc tgtaagtgtt gtatggaaga tgttaacgta
     5461 taataagctt gttctacttt tcgttttaca gcgttgggca actgcaatgt aactagttgc
     5521 aatcgttgtt gccaactctc aagggcttgt tctgtatagt ttattatttc ttcttttaca
     5581 gtatgtaaat tactttcggt ctgttgcata caatccacca aaaattgagc cacagccgtg
     5641 ggcgttttca aactggtatg tgccaccata tccaccaccg acacatcttt atcatgacca
     5701 atcccactca aaataggcaa cggaaactgt gtgcaatgat aggcaatgtt ataattatca
     5761 aaccaactca aatcagaagt agctccaccg ccacgtatga tgaccaccac gtcaaacaat
     5821 tctttgtgct catacacagc ttctagggca ttaataatcg aagcctctgt ttcagtgcct
     5881 tgcataacag cagcaaacaa tcgatgataa aacacaaacc caaacgcatt gccatgtaat
     5941 tgattacaaa aatctgtata acctgcagca ccaggcgaag aaataacagc aatacgttgc
     6001 acaagtaatg gcaaatcaat ctctttgttt aaatccatca ccccttcggc atacaattgt
     6061 tgaataatca aggctcgctt acgagccata tcgcctaagg tgtattgagg atcaatatct
     6121 tttattacca aactatatcc ataggcttca tgaaaactta cttctacttt taccaacacc
     6181 ttcatcccag ccataaggcg ttcgtgtgtg gttgtctcaa aataaggttt tatcatgcgg
     6241 taggtaaacg accaaattac cgctttggct ttggcaatga tatgctcatc agcatcgtct
     6301 ttttctatca actcgaggta gcaatgacct gaataatgtt cgtttacttc actaatttct
     6361 gcagtaatcc actgaggttg tgtattgtca gcaatcagtt tttttacaaa actattcagt
     6421 tcaactaatc gtatgtgtgc gtgtggaggc ataacataac cgacttatta gcgaaacaaa
     6481 ttaagatacg catctggttg gtcaagataa tcagcaggat cttttattgt tggatcgcct
     6541 tgttcgttct tatccattgt ctcttgcagt tttttatcca gttctgtctg attagtagct
     6601 ttcccatttt tatattctac agaggtaaac gaaccatcat cattgtatgt tttccattct
     6661 ccatgttttt tattggctac ataatagcct acttcgtctt tttgacccgt ttcataatat
     6721 gactcaaaag gaccatctaa caatccattt ttgtaaaaat agcttgccaa aatattgcca
     6781 tacggatgat attgaatacg ttttccatgc aacacattat tttcgtactg cattcggtca
     6841 attaccatac ccgactccga aaaaaccaac gactctcctt gcttcagtcc ttctgtataa
     6901 ttctctacca ttaccaagcg gttttcttta gaaaaatact cccaaatacc ttgtttttgt
     6961 ccttttacaa aaattccttt cgccaattga gaaccatcac cagcaaaata ctccactttc
     7021 acagtatcat tcgtggcaaa ttgttggata gctttcagtt tcccattttc gtgataccgc
     7081 tttattgttc ccacgggttt atcttctaca aaataccctt cgtacattaa atttccgttg
     7141 ggataatagc gatttttaaa aactgcttgt tgagtaaata acgaaataga aaataaaaaa
     7201 aaggcactaa ggcataaaaa ctgtttcata cgatcattca tttaattatg cgaagataca
     7261 ttatttttta caaaaaccat aattgacttg caataaatac acaccatgct cccatcaaaa
     7321 caggagatca atagacgaaa aaagcctaca tatttcaagt tatcgcattt aaatgttaaa
     7381 aacattctat ttttttcaaa ttcattctct ttttttagta aaaaacatgt ttctgaaaga
     7441 ttttttttta cttttgctat cgttgttttt tttacttcag ataagttgat aacaataata
     7501 ttctcactaa ccacaaaaat caatatgtac atgaaaaaaa acgtaccttt ttttctatcc
     7561 tcaatcaaac atcaagcggt aacatctcgt ttacgtcagc tctttttggg gattgcactt
     7621 ttttgcatcg ttccgctttg ggcagctgtt cagattaaca gtgtaaacac aagccacgta
     7681 tcttgtaatg gattatccga tggatcaatt acaatagttg ccactggagg ctcgggagta
     7741 tttcaatata gtttggataa ttttgtaaat tctattcaat cgaatagtac atttattaat
     7801 ttacctgcta ctaatactaa ttattcggtg tatgtacgtg atgccaataa tcatactgat
     7861 aatacatggc aaacagtaag catcacacaa cctcttcccc tcactttcaa tccaaccaaa
     7921 gatttcattc aatccgtatc gtgccatgga gcatctactg gaaaaatcaa aattgaagca
     7981 gaaggaggaa atgctcctta cctatacatt attaattcgc ttgacggaag tatttcgtat
     8041 agtaatactg taggggtttt tgataattta cctgcaacta cctattttgt acaagtaaaa
     8101 gatgcgaacg gatgtattac caatcatgct gacatggtag ttccacaagc tgaattgctt
     8161 gaagctgagc tgatgtcgaa aacaaaaatt gtgtgtaaag gagatatgac agcctcagtt
     8221 cgttttaaaa taaaaggaag aacagaagct tgcggtttat taaaagccga tacaacggct
     8281 tattattcag caaaattgta taatattaca acgggggtag atgtgttgga aggcgaattt
     8341 acggtaacga atcaattgca tcctgtatta acccaaaata tccaaatccc tgtgttggat
     8401 gaatttggaa accctgtagt aacagaaact ggtaatccag taatgcaaac tgtaaaagat
     8461 acattatatg cagaaggttg ccacgaacca actaaaatgg gagtagtgcc cgcttatacc
     8521 gtagataaaa aaggatttga ttgcgatgat tatatttcta tcgggaaact aggagaatat
     8581 gcgtaccgtt tggagttata tagaagaaat tgtcaaatag gtacaaaatt tgaatttgtt
     8641 atcgaacgga gtggtgatat ccctacctta tcattaaatg gagtgaatcc gatatgcgat
     8701 ggttcttctg aagtaataaa agcaacgata gtagcaactc ctgccactac tacttacaaa
     8761 tggatattaa acgaaacaac cattgctact accaaagatt tgacccgcgt atttacgatg
     8821 gatgataatc aaggtaaact taaatttgaa gcaaaaaata cctgcggaac ggttgtttct
     8881 aacgaaattg ccttgtctgt tttacctcgt cccacaggta ttgtaactac cgagaaagga
     8941 tacctttgtc aaggacaatc atcaaacgta acagcctttt tagctggtaa agctccattt
     9001 acgtacacct tgactggcaa cacagaacaa accacctcta aggtgtcaga aacagtgagt
     9061 atgagtccta cctctactat tgacattaca tttgagtcgc ttagcgatgc caattgtgtt
     9121 gctacacctg ctgatatcac aggcacaagc attaccgtat atcctgcacc ttcgatagat
     9181 attacggtaa atgtaccttc accgatggtt agcggacgtt acgttacagc tacagtaaca
     9241 cctgggtttg tggattactc tttgtggata aataaccaag agataggagc attagcaacc
     9301 gaagaaaatg tgtttaaatc aaaaaacttc gtgtatggac tttcatcaaa tatttttgac
     9361 accaaagttg aagataccaa tggctgtatc tgggattttt ctaaaacaga agacattaca
     9421 gttgttcttt tcccaaatgt atttacgcct aacaacgatg gagtaaacga cgtatttctt
     9481 gaaggatata acctaaaagt attcgaccga ttaggcaccg tattgtactc cggaacaaaa
     9541 gggtggaatg ggatatacaa tggtgttcca gtaaacaaag gagtgtactt atatacagtg
     9601 gatatactcg acgaaaatgg agatactgtg attattaaaa atactgtagt agttgaaaaa
     9661 taagtgtaaa cgaaataaaa gtacattata tgaaaataac taaaaaaagt ttcctatttg
     9721 ccttgctggt atgcttgcca attatcagta tgatgggtaa tatgaaagtc ggaaacaata
     9781 aatacgaaag acgcgattac ttaggtgcaa ttgaacaata caaaaagact gctgaaggca
     9841 actcaaacga aaaaacagta gccaatgcta aatataaaat tggagaatgt tattccctat
     9901 taaatgaacc gaaagaagct gtttcttatc taggagaagc cattgctgga ggtgttccta
     9961 cattggaagc atacgtgcta tacggtaaaa acttactcaa aatgggagaa tatgcacgtg
    10021 ctaaagcttc ttttgaagaa ttcgacagac tttcgcccaa agatgctcgt attaaaaact
    10081 taattgcttc gtgcgatttt gcccttaaac acagctcaaa aaatcctatt actcccgagc
    10141 aagacttaaa aggaatcaat acctctgaaa ttgaatacgg aattggtttt tatcaagatg
    10201 gtatcgtata tgcctctgtt gaacaagcaa aaaaagggct atcatcacgt atgtattttt
    10261 cgcatggtaa agataatttc acctcatctc gcttggtgga tatgattaaa atacgaacag
    10321 gtcaaaatgt aggtacattt gccatcgaca cacttgataa catcttatat tacacccgtt
    10381 gtaacaataa cgagagagac aattgctaca tctacacagc ccgttataaa aaagggaaat
    10441 ggaaagaaaa gggagcttta aatattggca acagacatgt gaatgttgct caccctgccc
    10501 tttcgcccga cagacaacgc ttgtatttta cagcaaattt agaaggggga tttggtggaa
    10561 cagatatttg gtacgtaacg cgtaacgaaa acggaaagtg gaacaaaact cccatcaatg
    10621 ttggagccgt agtaaacaca gctggcaacg aagctttccc ttatgtagta ggcaatacgc
    10681 tcatatttgc atcagacgga catattggat ttggtggata tgatttgtat agtgcacaaa
    10741 tagatggcaa agccgtacat aaagtgcaaa acttgatgaa acccatcaac tcatcagccg
    10801 atgacattaa cttaattgtg tcagaagaaa aaaatgaagc tcttttagta agtagtcgca
    10861 atatttctac caaagatgat atattccgct tcgaaggtat ttttaccagc atgatggtat
    10921 cggggcatgc ttacaataat gtaacaaaag aagtactccc aaatacacaa gttgtattat
    10981 cagggatggg taaaacacac accactgaaa cgaataaaga tggatattat tatgcattta
    11041 tcgaagcagg cgacttatac aaaatgtttg ctaatgcagt aggattttta tcggatatgg
    11101 caatgataaa ggcagaaaag accactattg gttcatttcc tgtagaacaa gatttttatc
    11161 ttacaccagc tagtatgtca attagcggac gtgtatatga tatggaaacc aacgagccat
    11221 ttatcaacga agatgtgcta ttgttagaaa atggcacctt ggctcaacaa acaaaaacag
    11281 atatcacagg acgttattcc tttactaatt taaccaatgg aaaagaatac caagtaaaag
    11341 tgaacaagcc aaacttcttg tctattacaa gtaaaccttt tatttaccaa gaaggagatg
    11401 caaacaatag ctcgttcgat ttagcttcca ttccttacgg ttcagattcg tttgatccaa
    11461 gtggtgttgg tggtgttggt ggtatgggta atacaagcaa tgttggaggt ataaatggag
    11521 gacgatttgc tggtcgcgaa atttcaataa acgaaattta ttacgatttc gacaacgcta
    11581 acttgcttcc acaatcgcgt ttttcattag acaaaattat tttattatta agctctaacc
    11641 caaccttaaa acttgaattt ggttcgcata ctgacacacg tggtagtgct gaatacaacc
    11701 tacaattatc taatgcacgt gcaaaatctg tggtggacta tttaattgct tcgggcatta
    11761 gtcgcaacag actttcgtgg aaaggatatg gtaaaaccaa tcctatagtg aaaaatgctg
    11821 ttacagaaga agaacatcga ttaaacagac gtaccacatt caaaattatt gaacgataac
    11881 tttaaacatg aacacaatga aaaaaatact tatagcttgc tgtgcatccc ttctgttttc
    11941 tttgtcagtt tttgcacaac acgatgcaca aatcagtcaa catttgctga gtaaaacgca
    12001 ttacaaccct gctgcagcag gagcttcgtt atacggtaac attacaggta ttgctcgtca
    12061 acagtatgtg ggctttacag gagcaccgca aactggtttg ctaaactttg atatgttact
    12121 cccaaatacc agctctggta tagggttatc agtagtgtat gataagtatg gaccgctatt
    12181 atcgtataat ccaatgatga actatgcgta tcatattaaa ttgggcgaca accaacatct
    12241 ttcgttagga gtagccgcag gggtgttttt acgccaatat gatgcatcaa acaatattta
    12301 cgaaaatact ggagaccctt tagaaaatta tgatggagct caagaatact tagcaccaga
    12361 tgccaacata ggtatcgaat accaattaga aaactggatt attggggcat cagctaccca
    12421 tatacctcaa ttttttgaag aagaaaccgt aatgaatccc acaacacagt attatgccta
    12481 ctcgcgttat cgctttgaaa ttggcaggta ctgggattta actcctggca ttgcagctca
    12541 atacgatggc tactctgtac atagcgaaat aaatgcggta ttgcattacc taaaattatt
    12601 ttgggtaggt ggctcgtacc gatttagcga tgtattcgac tctgaatcaa ttgtgcctat
    12661 gataggactt caattaagcg actatgtacg cttaggatat gcgtacgatt acaatttatc
    12721 gagcttacaa aactacaacc aagggtcgca cgaaattatg ctacaactac gtttcaaaac
    12781 acggcaccat acatcacgaa caccgagatt tgctgaatgg taagaaagta gaatacattt
    12841 taactcaaaa agacgctgaa aataacacag cgtctttttt tttgtaaaac aagactgtaa
    12901 aaatgataca taaataaaaa aagcattgta tatttgtgtt attaagaaaa acgatatatg
    12961 gcagatattt tagatacgaa agaacaagca gaaaatgaac ttattgagca agaatatcag
    13021 ttacttatta aagaatatct tgcctctaac catagaaaaa aagttgctcg tatagaaaaa
    13081 gcttttttac tggctaaagc tgctcataaa ggaataaagc gtaaatcggg agagccttac
    13141 atcttacacc ctttggctgt agcacgcatt gttgctaagg agattggact cggttctacg
    13201 tctatttgtg ccgcgttgtt gcatgatgtg gtagaagata ctgaatatac cgtagaagat
    13261 attgcgaatt tgtttgaccc caagattgca caaattgtag atgggttaac caaaatatca
    13321 ggtggtgttt ttggtgaaaa tgtgtcggga caggcagaaa attttcgcaa actcctgctc
    13381 accatttcgg aagatattcg ggtagtttta attaaaatag ccgaccgttt gcataatatg
    13441 cgtacattag gctcgatgcc tgccgccaaa caatacaaaa tagcaggcga aaccgaatac
    13501 atatatgctc ctttggctca tcgattaggc ttgtttgcta tcaaaacgga attagaagat
    13561 ttaagtttta aatacaattt cccgtctgtt taccaagaaa ttagtgaaaa aatcaatgaa
    13621 aatcaacttg aacgtatgct tttatacgac aaattctcgt tgcctatcat caataaactc
    13681 aacagcttag gatacaaata ctccatacga gcacgcatca aatcagtgta ctctgtgtgg
    13741 aataagatga aaaagaaaaa tctacctttt gaagaaatat acgatatctt ggctgtacgt
    13801 atcgtatttg aacccaatca gaatgtagat gaaaaagaac aatgctggat tatttacaac
    13861 aatattacca acctctatat tcctcatccc gataggttga gaaattggct caacacaccc
    13921 aaagccaatg gttatgaagc cttacacgta accgtaatgg gtccagatgg gcaatggata
    13981 gaggtgcaaa ttagaagtgc ccgcatggac gatattgctg aaaaaggatt ggctgcacac
    14041 tggaaataca aacaacagtt ttatggcgat tcggaattag ataaatggct aaacacgatt
    14101 aacgatatct tgaaaaatcc aagccccaat gccatggact ttcttgatac cttcaagctt
    14161 acgctgtacg ctaccgaaat atttgtattt acacccaaag gcgaaataaa aaccttaccc
    14221 tcaggctcca ctgccttgga ttttgccttc gaaattcact ccgatattgg tatgcgatgt
    14281 attggagcga aagtaaacaa caaactagta ccattgagct acgaactaca aagtggcgac
    14341 caaatagaaa tacttacatc aaaacaacaa acacctcaac cagaatggct tagctatgtt
    14401 acaacagctc gagcaaaaca gaaacttaat ttgcttttta aaaacgaaca aaaaatagta
    14461 aaaacagaag gggaaactat cctacgcaaa gcacttacag aagctggatt tgtaataaaa
    14521 aaagatatta gcaacgatat aatcaaaaaa ttgttgcacc attttgaaat caacagtttt
    14581 gaagaactta cttttcaaat aggcaaaggc agtatttctc ttgccaatat tcaagatatt
    14641 gtaaaacagg agaataaaaa aaccaccgtg cgttcgtggt atcctcgctt cttctttggc
    14701 aaagatacgg ataaagagaa tacagcaaca gcttcggctc gtattaaaaa gaatggcacc
    14761 taccagctta cagatgaaat gctgcaaaaa gaacacacta ttgctccg
//
LOCUS       DAOSRP010000036        14606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4202_length_14606_cov_5.153185, whole genome shotgun sequence.
ACCESSION   DAOSRP010000036 DAOSRP010000000
VERSION     DAOSRP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14606
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4202_length_14606_cov_5.153185"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLJ82_03550"
     CDS             <1..454
                     /locus_tag="PLJ82_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOR40613.1"
                     /translation="YLETLFQYPFTNKNSIALMGVGAYAHTAIELLQKDTTVKVAIAL
                     NPITDYAHYLSVLSEGWMGLPQKNFMAYQQAELSSLANYRGKLLLMHNVDNTDVHIQH
                     TWLLVDALIKANQQFDMQLYPSIYYDNVTQTLYLHSFERIMSFLNKNM"
     gene            536..1444
                     /locus_tag="PLJ82_03555"
     CDS             536..1444
                     /locus_tag="PLJ82_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOR40614.1"
                     /translation="MKPTLYVMAAGMGSRYGGLKQMDGLGPSGETIIDYSIYDAIRAG
                     FGKVVFVIRKAFEADFRKIVISKFENKIPVEVVFQEVDKLPEGFSVHPERVKPWGTNH
                     AILMGKDVVKEPFAVINGDDFYGKESFEVLGKALQQMEGKSAQYCMVGFPVKNTLSES
                     GSVSRGVCQTDNNNNLVSVVEHTKIEAQNGRIVNLNDLGQELVVLDPNTPVSMNMWGF
                     TPDYFEFSEAYFKEFLKENEKSLKAEFYIPSMVDYLIKNNIATVKVLDTPSKWFGVTY
                     ADDKATVLMKLNELIHKGVYPAKLWS"
     gene            1459..1923
                     /locus_tag="PLJ82_03560"
     CDS             1459..1923
                     /locus_tag="PLJ82_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOR40615.1"
                     /translation="MKKRNYLIGFILVLLVSCICKEPTEPVVVEDDQIVGTWYIHDYT
                     ISNVSVFAIQQCDVGSWIELKADNTMQALIKCNTPPESEGTWSREGDVINIDADKLFN
                     VNKALLSRIDNQGTVLLLTDEELQFEFAVYTTLNTKQMVKVFLSRTPLETVE"
     gene            complement(1936..2652)
                     /locus_tag="PLJ82_03565"
     CDS             complement(1936..2652)
                     /locus_tag="PLJ82_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40616.1"
                     /translation="MKKIIVICLSCFFGSLFTIHAQLVKQKVGGDAKHQLTLLGGINF
                     PSFDIPHSIGPSAGLAYSYYFIPQIGIRGMLHYHYFDGEKNIPAIYSHTMGACVQGIF
                     NFFSYQSGRPEDAGFSFIPSKAYLVAGVGGLYHQPYINGNSQQSDLSFSIPVGIGTRH
                     PIARRWDIGVEFAWNVSSSDRIDQQVVSESTNDSFGNLFLTATYKFPKHNYGPTRGKQ
                     FQNRKTKKCDKRKGCAVAFD"
     gene            2787..4064
                     /locus_tag="PLJ82_03570"
     CDS             2787..4064
                     /locus_tag="PLJ82_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR40617.1"
                     /translation="MKKVLIISYYFPPSGGSGVQRWLKFVKYLPKFGYEPIVLTVDPT
                     VASYPQIDTTLLQDIPSLVRVERTTTREILSLYKRVSPTKEIPYGGFANENTNTFFSI
                     LSRFVRGNLFLPDPRRGWNKFAYKKACELIEQEQIDTVITTSPPHSTQLIGLKLKKRF
                     SSIQWIADLRDPWRDIFYYKDTYPTWLADKLNAYYERAVLQQADQLITVSNGCKNLFL
                     QHAAVADKFHVIPNGYDEDDFEQLPVDKTANKFVISYIGVLSGLYCIDGLIDAVANLS
                     ASYKHQLIIRFVGKIAPEIRQKLSEKGIDDILEFVDYVPHNEALRYMKNAHLLLLLLP
                     HASSNTGILTGKLFEYMASETPILMLASDTEEATQLLHSCQAGRAVHPLDSVAIQLAI
                     ENAIDGKWGKASYKLYTDYSRRALTGRLASLIS"
     gene            complement(4217..5674)
                     /locus_tag="PLJ82_03575"
     CDS             complement(4217..5674)
                     /locus_tag="PLJ82_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOR40618.1"
                     /translation="MSNSSLKDKMIGALAWSSVDRVAQQGIQFIFGIILARLLAPDDF
                     GLLGMIMIFATLSYVLVESGFSAALIRKEDANETDYNSVFYVNLAISVALFVVLFFTI
                     PYFAEFFKQPALVSTARITFIAVIFNAFYLVPYVQVNRAMDFKTLAKINLFATLCSGS
                     IGITLAILDYGVWALVYQQIGYQFFRGIGFYALVRWIPSFTFSLDSIKHLWKFSMHIL
                     GSSLLTIGFNNLYTFLLSRFYPIKQVGYYTQANKMSETANFTFQAILASSTYNLFTQI
                     QADKERLLRVYRQLIQHISLVVIPVSLVLMVVAKPLFFVLFSEKWLPAVPYFQLLCVA
                     NVFAPLYLVNMNVLNSLGESKRTFYIEIIKRLFILISIVVCFQFGIQALLAGYAIACT
                     MAFVFSTIYIKKHLRHYFFHQLNDVIMPIMIGISIALIAWSMNSLISNLYLLLVIQLL
                     LALVVYIIILRFFCKEKLYGFYDYIKRQSKQLTKK"
     gene            complement(5697..8453)
                     /gene="leuS"
                     /locus_tag="PLJ82_03580"
     CDS             complement(5697..8453)
                     /gene="leuS"
                     /locus_tag="PLJ82_03580"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOR40619.1"
                     /translation="MEYTFKEIEQKWQQYWKNNATYKVEINATKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIFSRFKRLKGYNVLHPMGYDAYGLPAEQYAIQTGQHPAITTTK
                     NIARYREQLDKIGFSYDWNREVRTCEPDYYKWTQWAFVKMFNHYYDTQLQQAKPIENL
                     VKHFATQGTDSLQAACTEELSFTAKEWNEKTDEEQQHILLNYRIAYLADLKVNWCPAL
                     GTVLANDEVSEGVSIRGGHPVEQRIMRQWSLRISAYAQRLLDGLDTIDWSESIKETQR
                     NWIGRSEGAEMKFPIKGQDANLLIFTTRADTTYGVTFMVLAPESEYVQQLTTAKQQQA
                     VETYIESVKKRTERERIADRKVSGVFTGSYAINPFSQQEIPIYISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIVPVIEGCDVSEESFDAKDGVMCNSGFMNGMTVKAAIET
                     AKKYVSDNGIGRVKVNFRLRDAIFSRQRYWGEPFPVYYKNGLPYMLDESKLPLELPEI
                     DKYLPTETGEPPLGRAKAWHTAEGYPYELNTMPGFAGSSAYFLRYMDSRNSNELVSKE
                     ANNYWQQVDLYLGGTEHATGHLIYSRFWNKFLFDLGLICKDEPFKKLVNQGMIQGRSN
                     FVYRIKDTNTFVSYHLKDQYEVTPIHVDVNMVSNDILDIERFKAWNPEYNKAEFILED
                     GKYVCGWAVEKMSKSMFNVVNPDDIVEKYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFLRKLWSLFYKDGNYIVTQDTPTTEELKTLHKTIKKITFDIENFSFNTSVSAFMIC
                     VNELAQQKCSKQAILEPLVILIAPFAPHIAEELYHALGHTTTVCDAVFPICNESYLVE
                     STINYPISFNGKVRFTLPLEASLSKEEVEKAALQNEQTRKFLQDKQPKKIIVVPGKIV
                     NIVL"
     gene            8627..9718
                     /gene="recF"
                     /locus_tag="PLJ82_03585"
     CDS             8627..9718
                     /gene="recF"
                     /locus_tag="PLJ82_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759932.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOR40620.1"
                     /translation="MVLTKLSIVNFKNIADASLDFCAGINCFIGNNGMGKTNLLDAIY
                     YLSFCKSHTNAIDSQNVKHDEGFLMLQGNYIHQQQEEEIYVGLKRGQKKLFKRNKNAY
                     ERISDHIGLIPLVLVSPSDAVLIDGGSDERRKFIDGVLSQYDKKYLDCLLKYNYVLQQ
                     RNALLKSNAVEETMYEVCEMQMAHFADYIYAQRTQFISQFLPVFQQYYVAIANVNEQV
                     SISYKSHIEDGDLTIQLKQSRAKDTLLGFTTKGIHKDELEFYLGDYLVKRVGSQGQNK
                     TFLVALKLAQFEFLKQVHACNPLLLLDDIFDKLDANRVSKIIQMVSGDNFGQIFITDT
                     NREHLVSILQQLPQENRIFLVNEDGINKE"
     gene            9725..10015
                     /locus_tag="PLJ82_03590"
     CDS             9725..10015
                     /locus_tag="PLJ82_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOR40621.1"
                     /translation="MRRQNTEKIDDVIKQIFQQQNLQPKLDEVDILQSWETVLGPTVK
                     KYTSDLVIKNKVLYVKLSSSVLRNELMMSKSRLLQALNDTVGKEVITDIVLR"
     gene            10120..10695
                     /locus_tag="PLJ82_03595"
     CDS             10120..10695
                     /locus_tag="PLJ82_03595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOR40622.1"
                     /translation="MTFELKKLPYDPTTLNPVVSPKTFEFHHGKHLQAYVNNLNNLKT
                     GTKFENMDLISIIKESEGPIFNNAAQIFNHEFYFDQFAPSPKSKPEGALATAIDAAFG
                     SFDNFKKEFSAAAVSVFGSGWAWLVVNAAGTLEITKESNAGTPLTKNQKPLLTFDVWE
                     HAYYIDFQNRRPDHVAALWDVIDWKVIESRF"
     gene            10710..11381
                     /locus_tag="PLJ82_03600"
     CDS             10710..11381
                     /locus_tag="PLJ82_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOR40623.1"
                     /translation="MSTIAQNIEFLRKQLPNHVRLVAVSKFNPAESVMEAYRVGQRVF
                     GESQAQELVPKAQLLPKDIEWHFIGHLQTNKVKYIIPYVSLIHAVDSLKLVQEINKQA
                     LQQHRIMDCLVQIRIAQEETKYGMTLEESVSMLHYISVNNFTAIRICGVMGLATDTED
                     QNQVTKEFQYLTYCFHTLKNTFFQTSLYFSEISMGMTHDYPLAIEQGSTLIRVGSGIF
                     GERVY"
     gene            11529..14249
                     /gene="ppdK"
                     /locus_tag="PLJ82_03605"
     CDS             11529..14249
                     /gene="ppdK"
                     /locus_tag="PLJ82_03605"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOR40624.1"
                     /translation="MDKKRVYFFGNKTAEGNGSMKELLGGKGANLADMNLIGVPVPPG
                     MTITTEVCNEYFLIGQEEVIKTIKPEVEKAMKQIEDAVAGPKFGDNKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEAVETIAQKSGNPRFAWDSYRRFVQMYGDVVLGMKPESKQ
                     DEDPFEVIIEKMKHEKKVELDTQLTTEDLKELVAKFKKAVKDVTGKDFPVSPWEQLWG
                     AVMAVFNSWMNERAILYRKMNKIPAEWGTAVNVQAMVFGNMGNNSATGVAFTRDASTG
                     EDIFNGEYLINAQGEDVVAGIRTPQEITIEGSRRWAKMQGISEADRLAKYPSLEETMP
                     SAYADLNAIQQKLEDHYTDMQDIEFTIQDGKLWMLQTRNAKRTGAAMVRVAMEMLKQG
                     IIDEKTALLRCEPEKLDELLHPVFDTKALAQAKALSQGLPASPGAATGQVVFFAEDAE
                     AWAAEGKKTILCRVETSPEDLKGMYAAEGILTARGGMTSHAAVVARGMGKCCISAANG
                     LVIDYKTRTMTVGGATIKEGEWVSLNGTTGILYAGKVETKAAELSGDFAELMKIADKY
                     ARLQVRTNADTPHDAQVARNFGAQGIGLTRTEHMFFDGDKIKAMREMILAQDEAGRRK
                     ALEKLLPLQREDFEGIFEAMNGLPVTVRLLDPPLHEFVPHDPKGQLEMAHEMGLNVDE
                     IAARVHALSESNPMLGHRGCRLGNTYPEITETQTRAIIEAALNLKKKGIVAIPEIMVP
                     LTGILHEFTAQKTIIDATAKQVFEERGDSIEYKVGTMIEIPRAALTAQRIAEQAEFFS
                     FGTNDLTQMTFGYSRDDVAKFLPIYLEKGILKNDPFQVLDQKGVGQLITMATVNGRAV
                     RPNLKVGICGEHGGEPSSVEFCHRTGLNYVSCSPFRVPIARLAAAQAAIR"
BASE COUNT         4737 a         2554 c         2789 g         4526 t
ORIGIN      
        1 gtatctagaa acactttttc aatacccatt tactaataaa aatagcattg ccctgatggg
       61 ggttggtgca tatgcccata cagctattga gttattgcaa aaagacacta ctgtaaaagt
      121 tgctattgcc ctgaatccaa ttaccgatta tgctcattat ttgtcagttt tgagtgaagg
      181 ttggatgggt ttgcctcaaa agaattttat ggcttaccaa caagcagagc taagctcttt
      241 ggctaattat cgtggaaaat tattactcat gcacaatgtt gataatacgg atgtgcatat
      301 acaacacact tggttgttag tagatgcttt gatcaaggca aatcaacaat ttgatatgca
      361 attatatcca tcaatctatt atgataatgt aacacagacg ttatatctgc atagttttga
      421 acgaattatg tcttttttaa ataaaaacat gtaaatagca atcaaaacgt aagctttttt
      481 tgctactttt gtcaaacaaa ttttattagt agaatatatt aacacagcaa acaagatgaa
      541 acctacattg tatgtaatgg cagcgggcat ggggagccga tatggcggat taaagcaaat
      601 ggatggctta ggtccaagcg gagaaacaat tattgattat tctatttacg atgccattag
      661 agctggtttt ggcaaagtgg tatttgttat tcgcaaagcc tttgaggctg attttagaaa
      721 aatcgttatt tcaaagtttg agaacaaaat tcctgtagag gttgtttttc aagaagtaga
      781 taaattgcct gaaggtttta gtgttcatcc cgaacgagta aaaccatggg gaaccaatca
      841 tgctattttg atgggcaaag atgtggtaaa agaacctttt gctgttatta atggagatga
      901 tttttatggt aaagaatcat ttgaagtatt aggaaaagcc ttacaacaaa tggaaggaaa
      961 atcggctcaa tactgcatgg tgggatttcc tgttaaaaat accttgtccg aaagtggttc
     1021 agtatcacgt ggtgtgtgcc aaacagataa caataataat ttggtgtcgg tagttgagca
     1081 taccaaaatt gaagcacaaa atggcagaat cgttaatttg aatgaccttg gacaagaact
     1141 agtcgtatta gatcccaata cgcctgtatc catgaatatg tggggattta cacctgatta
     1201 ttttgagttt tcagaagctt atttcaaaga atttttaaaa gaaaatgaaa aaagtttaaa
     1261 agctgaattt tacattccaa gtatggtcga ttaccttatt aaaaataata tagctaccgt
     1321 aaaagtgtta gatacaccgt ctaaatggtt tggagtaacg tatgctgatg ataaggcaac
     1381 ggtattaatg aaactaaatg agttgattca taagggagta tatccagcaa aactatggag
     1441 ctaacactcc cataaaccat gaaaaaaaga aattatctta ttgggttcat ccttgtattg
     1501 cttgtttctt gcatttgcaa agaaccaacg gagcctgtag ttgtagaaga tgatcagatt
     1561 gtgggcacat ggtatataca cgattatact atttctaatg tgagtgtatt tgctattcaa
     1621 caatgtgatg tgggttcgtg gattgagttg aaagcagata ataccatgca ggcactcatt
     1681 aaatgcaata cgccacctga atcagaagga acatggtcga gagaaggaga tgttatcaat
     1741 attgatgcgg ataaattgtt taatgtaaat aaggctttat tgagtaggat tgataatcaa
     1801 ggaacagttt tacttttaac agacgaagag ttacaatttg aatttgctgt ttatactacg
     1861 ctgaatacta aacagatggt aaaagtattt ttatctcgta ctccattaga aacggtagaa
     1921 taaaaaatta tttctttaat cgaatgcgac agcacatcct ttgcgtttgt cgcatttttt
     1981 tgttttgcgg ttctgaaatt gtttgccacg tgtaggaccg taattgtgtt ttgggaattt
     2041 gtacgttgca gttaaaaata agttaccaaa cgaatcattt gttgattcac ttactacttg
     2101 ttggtcgatt ctatcagatg aggaaacatt ccatgcaaat tctacgccaa tatcccacct
     2161 gcgagctatt ggatgtcggg taccaatacc tacaggaata ctaaaagata ggtcgctttg
     2221 ttgcgagttt ccgtttatgt aaggttgatg atataatcct cccacgccag ccacaaggta
     2281 cgctttgctg ggaataaatg aaaatccagc atcttctggt ctgcccgatt ggtacgaaaa
     2341 gaaattaaaa ataccttgta cacacgcacc catagtatgc gaatagatgg caggaatatt
     2401 tttttctcca tcaaaataat gatagtgcaa cataccccta atgccaattt gtggaataaa
     2461 ataatacgaa taagcgagtc cagccgatgg accaatgcta tgtggaatat caaaacttgg
     2521 aaaattaata ccccctaaca acgtaagttg atgtttagca tcaccgccca ctttttgttt
     2581 aactagttgt gcgtggatag tgaataggct gccaaaaaaa catgaaagac aaatgacgat
     2641 gatttttttc atagataaca gtacgattag cgacacaaaa atacgttatt tatttaatat
     2701 cattaacaag caagcagatt tttttatcaa ttagatttac tatcgctcgt aaatgtgtac
     2761 atttgcacag tatatttgct aggcatatga aaaaagtgtt gattattagt tactatttcc
     2821 cgccaagtgg cggctctggt gtgcaacgat ggctcaaatt tgttaagtac ctccccaaat
     2881 ttgggtacga gcctattgtg cttactgttg accctacagt agcttcttat cctcagattg
     2941 ataccacttt gttgcaagat ataccctcat tggttcgtgt agaacgcaca accaccagag
     3001 aaatattatc cttgtacaag cgcgtttctc ctactaaaga aattccatac ggaggttttg
     3061 ccaacgaaaa tacgaacact tttttctcta tcctttctcg ttttgtgcga ggcaatttgt
     3121 tcttgcccga cccgcgtaga gggtggaata aatttgctta caaaaaagcc tgtgaactta
     3181 ttgagcaaga gcaaattgat acagtaatta cgaccagtcc gcctcattct acgcaattaa
     3241 ttgggttgaa gttgaagaag agattttcct ctatacaatg gatagccgat ttacgcgacc
     3301 catggcgaga tattttttat tacaaagata cgtatcctac atggttggca gacaaattga
     3361 atgcgtatta cgaacgtgca gttttgcaac aagccgacca attgattacg gtaagcaatg
     3421 gctgtaaaaa tttgtttttg caacatgcag cagtcgccga taagtttcat gttataccca
     3481 atggttacga cgaagatgat tttgagcaac tacccgttga taaaacagcc aacaagtttg
     3541 tgatttctta cattggcgtg ttaagtggtt tgtattgtat tgatgggttg attgatgctg
     3601 tcgctaattt gtcagcaagt tataagcatc aacttataat acgttttgtc ggtaaaattg
     3661 ctcccgagat taggcaaaaa ttgagcgaaa agggaataga cgatatactt gaatttgttg
     3721 attacgtacc ccataatgaa gcactacggt atatgaaaaa tgctcattta ttactgcttt
     3781 tgttacctca tgcgtcttcg aatacaggta tccttactgg caaactcttt gagtatatgg
     3841 caagcgaaac tcctattttg atgttagctt ctgatactga agaagccacc caactgctac
     3901 atagttgtca agctggaaga gctgttcatc cattggacag tgttgctata caacttgcca
     3961 tagagaatgc gatagatgga aaatggggta aagcaagtta taagctttac acagactact
     4021 ctagacgagc attgacaggg agattggctt ctcttatttc atagctggtt ttttatttat
     4081 tacacgaaga tacaccgaga agaaaaaaat aaaacctccg tgccactctg tgcaataatc
     4141 tcacaagaag acgcatgacc cactaaacac tcaacagtac acactctgtt caaccttcat
     4201 aaacgctcct catttttcac tttttcgtca gttgttttga ttgtcgtttt atgtaatcgt
     4261 agaatccata tagtttttct ttgcagaaaa aacgcaaaat aataatgtac acaaccaaag
     4321 caagtagtag ttgaataact agtaacagat ataggttgct aataagtgaa ttcatactcc
     4381 atgcaataag agcgatagaa atacctatca tgataggcat tatgacatca tttagctgat
     4441 gaaagaaata gtgtcgcaaa tgttttttta tgtaaatggt tgaaaaaaca aaagccatgg
     4501 tgcaagctat agcgtatcct gctaataatg cctgaatgcc aaattgaaaa caaacaacga
     4561 tagagataag aatgaatagt cgcttgatta tttcaatata aaaggtgcgt ttcgattcgc
     4621 ccaatgaatt tagtacattc atatttacta aatacaaggg agcaaaaaca ttggctacac
     4681 acaacaattg aaaataaggc acggcaggca accatttttc tgaaaatagt acaaaaaaca
     4741 aaggtttagc aactaccatt aatactagag agacgggtat taccacaagc gatatatgtt
     4801 gtatcagttg tctgtacaca cgaagtaatc gttctttgtc agcttgtatt tgtgtaaata
     4861 agttgtacgt actacttgcc aaaatagctt gaaaggtaaa attcgctgtt tcactcattt
     4921 tatttgcttg tgtataatat cctacttgtt tgataggata aaaacgactt aacaaaaagg
     4981 tgtataaatt attaaaacca atagtcagca atgacgagcc caatatgtgc atgctaaatt
     5041 tccataagtg tttgatgcta tccaaagaaa aagtgaaaga gggtatccat ctaaccaaag
     5101 cataaaaccc aattcctcta aaaaattgat agccaatttg ttgatatact aatgcccata
     5161 cgccataatc caatattgcc aaagttatac caatgctacc actacaaagg gttgcaaaaa
     5221 gattgatttt agccaaagtt ttaaaatcca ttgctcgatt cacttgcacg tatggaacca
     5281 agtaaaaagc attaaaaatg accgcgataa atgtaatgcg agcagtagaa accaatgcag
     5341 gttgcttaaa aaattcagca aaatagggta tagtaaaaaa caaaaccaca aacaatgcaa
     5401 cagaaattgc cagattgaca taaaaaaccg aattgtaatc ggtttcgttg gcatcttctt
     5461 tgcgaattag ggcagcacta aaaccgcttt ctaccaacac atacgagagg gtagcaaata
     5521 tcataatcat tcctaacaga ccaaaatcat caggagccaa aaggcgggca agtatgatac
     5581 caaaaataaa ttgaatgcct tgttgagcaa ctctatctac agagctccac gccaaggcac
     5641 caatcatttt atctttcaaa ctactgttgc tcatttagct tttggttagg cggtcgttat
     5701 agtacaatat ttacaatttt acctggtacc acaataattt ttttgggctg cttgtcttgc
     5761 aaaaatttac gtgtttgttc gttttgcaag gctgcttttt caacttcttc ttttgataga
     5821 gaagcctcta ggggcaaggt aaaacgtact tttccattaa atgaaatagg gtaattgata
     5881 gttgattcta ccaagtaact ttcgttgcaa attggaaaaa cagcatcaca aacagtagtt
     5941 gtatgcccca aagcgtgata caattcttct gcaatgtgcg gagcaaatgg agctattaaa
     6001 attaccaatg gttctaaaat agcttgtttg ctacattttt gttgtgccaa ttcgtttaca
     6061 caaatcataa atgccgacac agaggtgtta aacgaaaaat tctctatatc aaaggtaatt
     6121 tttttaatcg ttttgtgcaa ggtttttagt tcttctgtag ttggtgtatc ttgcgttaca
     6181 atatagtttc catctttgta aaacaacgac cataatttgc gtaaaaaacg atgaacaccg
     6241 tcaatcccat tggtatccca aggtttcgat tgttccaacg gacccaaaaa catttcgtac
     6301 aaacgcaagg tgtctgcacc atatttttct acaatatcat cagggtttac aacgttaaac
     6361 atcgattttg acattttttc tactgcccaa ccgcacacat attttccatc ttcaagaata
     6421 aattcagcct tgttgtattc aggattccat gctttaaatc gttcaatatc aagtatgtcg
     6481 ttgctcacca tattcacatc tacgtgtatt ggggtaactt catattggtc ttttaggtga
     6541 tatgaaacaa aggtgttggt atcttttatg cgatacacaa aattacttcg tccttgtatc
     6601 attccttgat tgactaactt tttaaaaggc tcatctttac aaattaatcc taggtcgaat
     6661 aaaaatttgt tccaaaaacg actataaatt aaatgccctg tagcgtgttc agttccaccc
     6721 aaatataaat ctacttgttg ccaatagtta ttggcttctt tgcttaccaa ttcgttgcta
     6781 ttacgagaat ccatgtagcg taaaaagtac gccgaagaac cagcaaaccc aggcatggta
     6841 tttagctcgt agggataacc ttcggctgtg tgccaagctt tagccctgcc aagcggaggt
     6901 tcgcctgttt cggtgggtaa atatttgtca atttcgggca attccaaagg caatttgctt
     6961 tcatcaagca tataaggtaa gccatttttg taatacacag gaaatggttc tccccaataa
     7021 cgttgacgac taaatatagc atcacgcaaa cggaaattca cctttacacg accaataccg
     7081 ttgtcgctaa catatttttt tgcagtttca atggcagctt tcactgtcat tccattcata
     7141 aaaccagaat tgcacatcac accatctttg gcatcaaaac tttcttcact cacatcgcac
     7201 ccttcgataa caggtactat gggtaggtta aaatgtttgg caaatgcata gtcgcgacta
     7261 tcgtgtgcag gcactgccat aatggctcct gtaccataac cagctaatac gtaatcgctg
     7321 atgtagatag gtatttcttg ttggctaaat ggatttatcg catacgaacc cgtaaatacg
     7381 cccgatactt ttcggtcggc aatacgctcg cgttcggtac gtttttttac tgattcaata
     7441 taagtttcaa cggcttgttg ttgcttcgct gtagttaatt gttgtacata ctcactttcg
     7501 ggagcaagta ccataaaggt taccccatac gtagtgtcag cacgggtggt aaaaatcaac
     7561 aaattagcat cttgtccttt aatagggaat ttcatttcgg caccttcgct tctgccaatc
     7621 cagttgcgtt gtgtttcttt aattgattct gaccagtcaa tggtatccaa cccatcaagc
     7681 aatcgttgtg cataagcaga aatacgcaaa ctccattgtc gcataatgcg ttgttctact
     7741 ggatgaccac cacggataga aacgccttcg cttacctcgt cgttggctag cacagtgccc
     7801 aatgcaggac accagtttac ttttaagtca gctaaatagg caatgcgata attcaacaga
     7861 atatgttgtt gttcttcgtc agttttttcg ttccattctt tggctgtaaa actaagttct
     7921 tctgtacaag cggcttgcaa tgaatctgtg ccttgcgttg caaagtgttt taccaaattt
     7981 tctataggct tggcttgttg taattgcgtg tcgtaatagt gattaaacat ttttacaaaa
     8041 gcccactgtg tccatttgta ataatcaggc tcgcaagtgc gtacttcacg attccaatcg
     8101 taactaaatc caatcttatc tagttgttcg cggtagcgag caatgttttt agtggttgta
     8161 atggcaggat gctgacctgt ttgtatggca tattgttcgg caggcaatcc ataagcatca
     8221 taacccatcg gatgcaatac gttatatcct tttaggcgtt tgaatctact gaatatgtca
     8281 gatgcaatat accccagcgg gtgcccaaca tgtagccctg ctcccgatgg atacggaaac
     8341 atatccaata catagtattt tggtttagta gcgttaattt ctactttgta tgtggcatta
     8401 tttttccaat actgttgcca cttttgttca atttctttaa atgtatattc cataatagct
     8461 tgtgagagta ttttcagtta tttagggcac aaagttaatg aaaatacagt gctttcccat
     8521 atttttctat cattttatat gtataccact tccttcgtaa acattgattc tattttttaa
     8581 cgcattcaat caaacttttt tgctattttt gcataaaaat tacagtatgg ttcttaccaa
     8641 attatccata gtaaacttta aaaatattgc agatgctagc cttgactttt gtgctggtat
     8701 taactgcttt atcggaaaca acggaatggg gaaaaccaat ctgttagatg ctatttatta
     8761 tctttctttt tgcaaaagtc atacaaatgc aattgatagt cagaatgtta aacatgatga
     8821 aggcttttta atgttgcaag ggaattatat tcaccaacaa caagaagaag aaatttatgt
     8881 ggggttgaaa cgtggtcaaa aaaagttatt taaacggaat aaaaatgctt atgaaagaat
     8941 atccgaccat attggattaa ttccattggt gttggtttcg ccaagtgatg ctgtgttgat
     9001 agacggtggt agtgatgaaa gacggaagtt tattgatggc gttctttctc agtacgataa
     9061 aaaatacctt gattgtttgc tcaaatacaa ttacgtgttg caacaacgca acgcattgct
     9121 taaatccaat gcagtagaag aaactatgta cgaagtgtgc gaaatgcaaa tggcacattt
     9181 tgcggactac atatatgctc aacgtaccca gtttatttct caatttttac cagtttttca
     9241 gcagtattat gtagctattg ccaatgtaaa tgagcaagtt agcataagct acaagtcgca
     9301 tattgaggat ggtgatttaa ccattcaact taaacaaagc agagccaaag atactctctt
     9361 aggttttacc acaaaaggaa ttcataaaga tgaactggaa ttctatttag gagattattt
     9421 ggtaaaaaga gttggctcac aaggtcaaaa caaaaccttt ttagtagccc tcaaattggc
     9481 acagtttgaa tttttgaaac aagtgcatgc ctgtaatccc ctattattat tagatgatat
     9541 ttttgataag ttagatgcaa atagagtaag taaaataata caaatggtat caggagataa
     9601 ttttggtcaa atttttatta cagacacgaa tagggaacat ttagtgagta ttttacaaca
     9661 gttaccacag gaaaatcgca tttttttagt aaatgaagac ggtataaaca aagaataacc
     9721 tactatgaga agacaaaata ccgagaaaat tgacgatgtt atcaagcaaa tttttcaaca
     9781 acaaaatttg caacccaaac ttgatgaggt ggatattttg caatcttggg aaacggtgtt
     9841 gggaccaact gttaaaaaat acacaagcga tttggtgata aaaaacaagg tattgtatgt
     9901 caaattaagt tcttcagtat tgcgtaatga attgatgatg agtaaaagtc gccttttaca
     9961 agcccttaat gatacggttg gtaaggaggt tatcacggat atagtactaa gataaaataa
    10021 cataaaattc ttgctttcgt tttgtttttt cactttttat acgtacattt gaaaatctat
    10081 ttaaacaaat ttgttaaaca attttaaaaa catacagtta tgacatttga actaaaaaaa
    10141 ttgccgtatg accctactac attaaaccca gtggtaagtc ctaaaacttt tgaatttcat
    10201 catggaaaac acttacaagc ttatgtaaat aatctaaaca acctaaaaac agggacgaag
    10261 tttgaaaaca tggatttgat ttctattatt aaagaatcag aaggtccaat tttcaacaat
    10321 gctgctcaaa tttttaacca cgaattttat tttgaccaat ttgcaccaag cccaaaatca
    10381 aaaccagaag gagctttagc tactgctatt gatgccgcct ttggttcttt cgataatttc
    10441 aaaaaagaat tttcggctgc tgcagttagt gtatttggtt cgggttgggc atggctagtg
    10501 gtaaacgctg ctggtacatt agaaattacc aaagaaagca atgctggtac accattaacc
    10561 aaaaatcaaa aaccattact tacttttgat gtgtgggagc atgcttatta cattgatttc
    10621 caaaacagac gccccgacca cgtagctgct ctttgggatg taattgattg gaaagtaatt
    10681 gaaagtcgtt tttaataaca cgaatacttg tgtctactat agcacaaaat attgaatttc
    10741 ttagaaaaca gctgccaaat catgtgcgtt tggtggctgt ttctaagttt aatcctgccg
    10801 aaagtgtgat ggaagcatat cgggtaggac aacgtgtttt tggcgaaagt caagcacaag
    10861 aattggttcc caaagctcaa ttattaccca aagatattga gtggcatttt attggacatt
    10921 tacaaacgaa taaagttaag tacattatcc cttatgtaag tcttattcat gctgtagatt
    10981 cgttaaaatt agtgcaagaa attaataaac aagctttaca gcaacatcgc ataatggatt
    11041 gtttagtgca aattcggatt gctcaagaag aaacgaaata tggaatgaca ttggaagaat
    11101 ctgtgagtat gttgcactat atttcagtaa acaattttac tgctattcgt atttgtggcg
    11161 tgatgggatt agccactgat acagaagatc agaatcaagt aacaaaagaa tttcaatacc
    11221 tcacatactg ttttcataca cttaaaaata ctttttttca aacaagtttg tacttttctg
    11281 aaatatccat gggtatgaca catgattatc ctcttgcgat tgaacaaggc tctaccttga
    11341 tacgtgttgg tagtggtatt tttggagaaa gagtctacta atttgcttat tttatttctt
    11401 ctttttagct aaataattgc taaacaaaac gctaaaattt ttattataat cagaaaaatt
    11461 ttatctttgc actctatttg ataaaatata cgtgtttatt caaaataatt ttttaattta
    11521 ttaattttat ggacaagaaa agagtttatt tttttggaaa caaaaccgct gaaggtaacg
    11581 gctctatgaa agagttactt ggtggaaaag gggctaactt ggcagatatg aacctgattg
    11641 gtgtgcctgt tcctccggga atgacaatta caacagaggt gtgtaatgag tattttttaa
    11701 tcggtcaaga agaggttatt aaaaccatta agccagaagt tgaaaaagca atgaaacaaa
    11761 ttgaagatgc tgttgctggt ccaaaatttg gcgataacaa aaatccttta ttggtatctg
    11821 tacgttctgg agctagagct tctatgccag gtatgatgga tactattttg aacttagggt
    11881 taaatgacga agctgttgaa actatcgctc aaaaaagtgg taatccacgt ttcgcatggg
    11941 attcttaccg tcgttttgta caaatgtatg gcgacgtggt actaggcatg aaaccagaat
    12001 caaaacaaga tgaagaccca tttgaagtaa tcatcgaaaa gatgaaacat gaaaaaaagg
    12061 tagaattaga tactcaatta acaaccgaag atttaaaaga attggttgct aaattcaaaa
    12121 aagctgtaaa agacgttaca ggtaaagatt ttccagtttc tccatgggaa caactttggg
    12181 gtgctgttat ggcagtattc aatagctgga tgaacgaaag agctattctg tatcgcaaaa
    12241 tgaataaaat tcctgctgaa tggggaacag ctgttaacgt acaagcaatg gtatttggta
    12301 acatgggtaa caattctgct acaggtgtgg catttactcg tgatgctagt acaggtgaag
    12361 atattttcaa tggcgaatat ttaattaatg ctcaaggtga agacgtggta gcgggtattc
    12421 gtacaccaca agaaattacc atcgaaggtt ctcgtcgttg ggctaaaatg caaggcatca
    12481 gtgaagctga tcgtctagct aaataccctt cattagaaga aacaatgcct tcagcttatg
    12541 ctgacttaaa cgctattcaa caaaaattag aagaccacta tacagatatg caagatatcg
    12601 aatttactat ccaagatggt aaattgtgga tgcttcaaac acgtaacgct aaacgtactg
    12661 gtgctgctat ggtaagagta gctatggaaa tgcttaaaca aggtattatt gatgaaaaaa
    12721 cagcattatt gcgttgcgaa ccagaaaaat tagacgaact tttacatcca gtatttgata
    12781 caaaagcatt agcacaagca aaagccttat ctcaaggttt acctgcttct ccaggtgctg
    12841 ctactggtca agtagtattc tttgcagaag atgcagaagc atgggctgct gaaggtaaaa
    12901 aaactatcct ttgccgtgta gaaacatctc cagaagacct taaaggtatg tatgctgctg
    12961 aaggtattct tactgctcgt ggtggtatga catcgcacgc tgctgtagta gctcgtggta
    13021 tgggtaaatg ctgtatctct gctgctaatg gtttggttat tgattacaaa actcgtacaa
    13081 tgacagtagg tggagctacc attaaagaag gtgagtgggt atcattaaat ggtactactg
    13141 gtattcttta tgctggtaaa gtggaaacca aagcggctga attaagtggt gactttgctg
    13201 aattaatgaa aatagctgac aaatatgcac gtttacaagt acgtacgaat gctgatacgc
    13261 ctcacgatgc tcaagtagct cgtaacttcg gtgctcaagg tattggtctt actcgtactg
    13321 aacacatgtt cttcgatggt gataaaatta aagcaatgcg tgaaatgatt cttgctcaag
    13381 atgaagcagg tcgccgtaaa gctttggaaa aattattacc attacaaaga gaagactttg
    13441 aaggtatctt cgaagcaatg aacggacttc cagtaacggt tcgtttactt gacccacctt
    13501 tgcatgagtt tgttcctcac gatccaaaag gtcaattgga aatggctcac gaaatgggct
    13561 taaatgttga cgaaattgct gctcgtgtac atgctctttc tgaatcaaac ccaatgttag
    13621 gtcaccgtgg ttgtcgttta ggtaacacat acccagaaat tactgaaaca caaactcgtg
    13681 ctatcatcga ggctgctctt aacttgaaaa agaaaggcat tgtagctatt ccagaaatca
    13741 tggtacctct tacaggtatc ttacacgagt ttactgctca aaaaactatt atcgacgcta
    13801 ctgctaaaca agtatttgaa gaaagaggcg attctattga atataaagta ggtacaatga
    13861 ttgaaattcc acgtgcagcg ttaacagctc aacgtattgc tgaacaagca gaattcttct
    13921 catttggtac taacgactta acacaaatga catttggcta tagccgtgat gacgttgcta
    13981 aattcttacc aatttatttg gaaaaaggaa ttttgaaaaa cgacccattc caagtgcttg
    14041 accaaaaagg tgtaggacaa ttaataacta tggctactgt aaacggtaga gctgttcgtc
    14101 caaaccttaa agtaggtatt tgtggtgagc atggtggtga gccatcttca gtggaattct
    14161 gccacagaac tggtttgaac tacgtaagtt gttctccatt ccgtgtgcca atcgcacgtt
    14221 tggctgctgc tcaagcggct attcgctgac tgacagaaga cattgaaaca atggaataaa
    14281 taaggtagag agcttgctaa tgtagcaagc tctctgtttt tttctactat gggtttttaa
    14341 agagaaaggt ctctcgttta tcgtatcgtt ttttaagact taacttggca tgtttataac
    14401 aaataaaatt cattgtacaa agacgatact acaaaaatgt ttatctttgc aaaagaacta
    14461 cagggtaaaa tcttgcggta actatacaga atcttttctg taaaaacaaa ggaataagtt
    14521 taaatctgta tttttgcagt tgcagtgaaa ttaacgatga accatacaaa actgtatcgg
    14581 cacggattac aaatccgcgc cagcgt
//
LOCUS       DAOSRP010000037        14604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4203_length_14604_cov_5.349646, whole genome shotgun sequence.
ACCESSION   DAOSRP010000037 DAOSRP010000000
VERSION     DAOSRP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_14604_cov_5.349646"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLJ82_03610"
     CDS             <1..450
                     /locus_tag="PLJ82_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR40625.1"
                     /translation="MAQQGDAYAFNLNKPRIGGYQYSFSGLKTSFLYLVRDEMKKNPA
                     FIEQRKTDLCASFQRTVVEILMEKLLKASQDYHISTIALAGGVSANSALRKAVENYAH
                     IYNWTTFVPKLAFTTDNAAMVAITGMYKFKAGEYSDISLSPFAKTLV"
     gene            458..1036
                     /locus_tag="PLJ82_03615"
     CDS             458..1036
                     /locus_tag="PLJ82_03615"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOR40626.1"
                     /translation="MQQSIKVTLLSWWEKLKPIHGVVYFVILLISCHFAWKMAFVEHT
                     EIPRKVTFLWFDVTQIFSFAVHNLTDVVYFLLKDILDVNIIKISNFFVYPDKAVIDIV
                     WSCSGFKQAYIFFTVIAFYPGKHIHKLWYIPLGLVVVYVFNVFRIALITYLLRDNLGW
                     FDVLHEGSKYVFYAIIFLIWVIWAEKFNKSSI"
     gene            1042..1770
                     /locus_tag="PLJ82_03620"
     CDS             1042..1770
                     /locus_tag="PLJ82_03620"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOR40627.1"
                     /translation="MNLVIDCGNSTTKIAVFDGENIISKSVVVTLHITDIASILHLYP
                     IKNAVISNVGIIPTDCIELIRKSVVVYIDLQSTTPIPLKNNYETPSTLGLDRLAVAVG
                     ANYLYPSRNILVIDAGTAITYEFIDEYNTYQGGSISPGLVTRFKSLHHFTQRLPLVQP
                     ENTIELFGRNTRAAILTGVINGLVYEIEGYITDFKQNYRDVLIFLTGGDIFYFDKKLK
                     SSIFVSENLLLIGLNRILNFNACL"
     gene            1761..3005
                     /locus_tag="PLJ82_03625"
     CDS             1761..3005
                     /locus_tag="PLJ82_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40628.1"
                     /translation="MFINKHIAIILSLTLISIHLVAQSGTVSPYSRYGYGNLRSGGFT
                     VSNSLGGVGYGLRSNSMINPQNPASYTAVDSLSFMFDFGASASFNQFTSGFSQQQKST
                     AQFDYLALQIPIIKNMGMSLGLMPFSTVGYAYSFSETLQFNADELLTKHTFEGNGGIS
                     RLYGGLAYAIMPQLSLGVNVNYLFGNIVHTRELTFPDNSTYSSSKEESNLYVSTFLWD
                     AGIQFIQPVGKKDVLVVGGTYTLKTPMNIRSEIVTTSTQTITDDTNYSFDYPQSIGVG
                     VSYKHGNSVVFAADYSYQSFADALFYSQRDTLLNARKLAIGCEYVPNSTSRSYVQTIR
                     YRLGANIAESYTKVNEKPYSEMGITLGFGLPLKNSKSILNLNLEYGSRGIINQNLVRE
                     DYVKIGLGVSINENWFFKRKFE"
     gene            3058..4404
                     /locus_tag="PLJ82_03630"
     CDS             3058..4404
                     /locus_tag="PLJ82_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40629.1"
                     /translation="MKAKLIFIGLVVLSVFTVNAQKGVDTGTRYGLGEDSIRCLENIS
                     LYQNYYHIKDYNTSFQHWRVVYTECPASSRDLYRIGSILLVWQMQNAQDVDKQKDYFR
                     QLMENYDQRIKYFGNDARFPESWVRGRQAIDYINYCPEDPLREKALPWLKYSIDERQA
                     DADADVIDGYMRVLDGLYRSDNEKYRDMYMTEYLRVGKILDTRIARACKFVDNYKLAQ
                     NNANALFIASGIANCTTLESVFASKVQESKSDIESLNTIISLFQSAKCTESEVYFDAS
                     SFAHVINPTAQSAAGIAYQSVKKEDFLKAVDYFEEATKLEKVDSVKYDYQYAIAAIYA
                     QMKRYTQARTHALRAATYNPKKGEPYILLAMMYADSNIFPDDKILQKTVYWAAVDKLE
                     RARSVDPSSRERAQQLINTYRQYFPTKEEIFFKPELRVGESFVVGGWINETVICRE"
     gene            4421..4969
                     /gene="lptC"
                     /locus_tag="PLJ82_03635"
     CDS             4421..4969
                     /gene="lptC"
                     /locus_tag="PLJ82_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOR40630.1"
                     /translation="MKAYSITVVILATVILFLEACTSSIQNDAPKVDRELMPVLKSYG
                     VSSVVSDSGVTRFRFNTPVWYVYDKAEEPYWDFPEGVHFEQFDTDLKVYAEVDADTAM
                     YYTNAQKWTLSGNVKAKNVAGEVFESERIYIDQKTEKIYTDRFIKITQQQRIITGIGF
                     QSNHTLTQYTITKPQGIFPIEQ"
     gene            4966..6231
                     /locus_tag="PLJ82_03640"
     CDS             4966..6231
                     /locus_tag="PLJ82_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR40631.1"
                     /translation="MTTTVFLIFISLLFSAFFSGMEIAFYVSNKLRFEINKQHSLLSS
                     SILSIFYANPSKYISIILVGNNIALVVYGIQMAKFLEPYLLLFLHNDLLIATLQTIFS
                     TIVILLTGEFLPKVLFRLNADLWLKVFAIPLFLCYLFLYPVATFVYQISIFLFKILRV
                     DVSNSAGGMTLGRADLDNFLQQNYENTTDKKHIENEVKLFQNALDFSKIKLRDCIIPR
                     TEILAVDFDTDIEELRVKFVESGYSRILVFKEHIDNMVGYIHSIELFKKPRKWQDQIR
                     VLPIVPETMAANKLMNYLLQKKKSMALVVDEFGGTSGMVTLEDIVEEIFGEIEDEHDT
                     QDYVAKKISENEYVFSGRLEIDYVQEQFDIQLPVSDEYLTIAGLILHYYQGFPKINEE
                     IIIGNYMCKVIKASANKIELIKLKVLATL"
     gene            6343..8418
                     /locus_tag="PLJ82_03645"
     CDS             6343..8418
                     /locus_tag="PLJ82_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOR40632.1"
                     /translation="MATLEKIRQKGVLLTVIIGGALLAFIIGGIDFNTMRQGARETVA
                     VINGEEIKIADYQKRIDEMTAFYKIELGQSNLSEEYVEQINNAVWNTWSNEQLIGKQA
                     QSVGLVVTDKELADAVYGDNIHPLFYSIRMFYNNQGQFDKTLLLQFLNSISQDTSGEA
                     AKYWGFIERMLKNNILEEKYYTLVSNSFNANDIDTKYAFDARTITTGLEFVAQPYFSL
                     SDSLFQVTNKEIKDRYKRDQIRYTQQDETRDIAIVTFDIKPSEQDFQEVKLWIEKLKN
                     EFSTSTDFVSIANQNSDVPTSDIAVSRNNIDPDLKEFAFTASVGDVFGPIYVNGTYKM
                     ARLSEKGILSSDSAKVRHILIQEPTVEATQKLVDSLFNVLKGGADFATVAQQYSKAGT
                     KAQGGELGWIKEGEIDKEFSTAAINAKVNEFFTLKLGSGIHIVQVTEKTKPIEKVKLA
                     SIIRKVEPSSRTFGVIFNQASQYIAQNRTIEKFEATANSENGLSVRTYTVSANDNKIA
                     NIKDSRQIIRWAYEKNEGDVTDKVFESGEQFVAVALKSVNPKGTKSLSKVTEEIKAEL
                     IKEKKADKLAADLTTKLNQAKNLNALGLPLQVVESVSFETPFVPSMGREPKVLGNIVS
                     LIKTQQPKVIKGNTGVFVVQAVNVGVQPAFNKKEEMNLVQQKMFSQNVLVESLKRTAD
                     IEDNRIRFY"
     gene            8571..10496
                     /locus_tag="PLJ82_03650"
     CDS             8571..10496
                     /locus_tag="PLJ82_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOR40633.1"
                     /translation="MRKIIGLIVSLLFISQFTFSQILTPVKWTFEISDIDDSSKEIVA
                     TASIDEHWKMYGTNLPEGGPISTVFIIEAITGASIEGDFLSSSQPKLYYDAMFQMELS
                     SYEKKAVFTQRLKIIDKANFSVKGYVRYMACDATSCLPPTEASFSFGSEPSQTITMQH
                     AIDTPFWEPVIDDLQLVGNGAVVQQQSYWIIFVFGFLGGLLALLTPCVWPMIPMTVSF
                     FLKSSKNKNKAIKNALIYGFSIIIIYLGLGLLVTGIFGASSLNSLATSAIFNILFFIL
                     LIVFALSFFGLFEITLPSSWVNAMDKKVDTTTELVSIFFMAFTLVLVSFSCTGPIIGT
                     LLVEAASTGSVVGPAVGMFGFALALAIPFAFFSLFPSYLQSMPRSGGWLNMVKVTLGF
                     LELALALKFFSVADLAYGWGLLSRDLFIGIWIIIFIILGLYYLRVIRFPMDKDDKKIT
                     LPRIIFALVSFLFVVYLLPGMWGKQRASISAFTPPMSTQIWNKNKDHVQAMFEDYDIA
                     MKYAQTVDKPVLIDFSGYGCVNCREMEATVFEDDAIKKMLKEDFIFVVLYVDDKKNLS
                     EPMLVSENNATRTLRTQGDKWSYLQRLKFGANAQPYYVVLNAAGKPVAPSYAFNKDVK
                     AFETYLKQAIKTYKNEK"
     gene            10486..12792
                     /locus_tag="PLJ82_03655"
     CDS             10486..12792
                     /locus_tag="PLJ82_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR40634.1"
                     /translation="MKSNHAEAIIEGLNSSQKEAVMYNNGPSLIIAGAGSGKTRVLTC
                     KIAYLLELGLSPERIVALTFTNKAAREMKERIASMVGQKIAKRLVMGTFHAVFSRILR
                     TESEHLTIKKDFVIYDTTDAKNTIKTIVKEMQLDEKMYKPALVLSRISYAKNNLLSPA
                     AYATSKLIDNDFYAKIPRVKDIYPVYVSRLKQSSALDFDDLLYYTNVLFRDFPKVKEK
                     YQDIFEFILVDEYQDINFAQHLIVKFLAEKHHRVCVVGDDAQSIYSFRGANIENILTF
                     KDTYPETRVFKLEQNYRSTQNIVGAANSLISKNKFKIKKDVFSENEPGEKIHVLTAYS
                     DIEEGYIVANKMQELYHQTTPNSWNDFVVLYRTNSQSRLIEDALRKQSIPYRVYGGVS
                     FYQRAEVKDVLAYIRLAINNDDEEAFKRIVNSPSRGIGNTTLAKVFAKAQLFQVSAFQ
                     VSANPMQYNVEVNAGTATKLINFTNLIHDFTLQMSQMDAYQVASYITRNAGIIRNLQE
                     SKTAENISALENIDELLSSIYEYAEQKKEEGEEHPSIVEYLSEISLLTDQDTGRDAEE
                     NRVTLMTIHLAKGLEFKNVFIVGVEEDLLPSVYADDEKGLEEERRLFYVAITRAEKRC
                     FISYSKSRFRNGQMTYAKHSRFISDIDSHFLHLPVDFSSKNTSHFDFSQQKESFFSSN
                     KPLTQTVIPKPTYNKNLTKLSAISNKTLSQPVLSEVKIGSRIEHERFGKGVIIAITGD
                     VADTRITVKFDNAGERVLLLKFAKFSLC"
     gene            12884..>14604
                     /locus_tag="PLJ82_03660"
     CDS             12884..>14604
                     /locus_tag="PLJ82_03660"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40635.1"
                     /translation="MVVRGFVLNEQQQPIEMVDVSVQQSPTRTVTDKFGKFEIHVSAQ
                     DSFFLVFSSIAHRPAIRKVYVNEKYVTNVTVVLQDVINELTDIEVKGEKHQHTTAEWV
                     NTEFIKVLPSASGKSIESLIKSFSGVSSTNELSSQYSVRGGSYDENSVYVNGIEIYRP
                     FLIRSGEQEGLSFINTDMVGEVTFSAGGFDARYGDKLSSVLDITYKQPSTFEGNVAVS
                     FAGASAYIGSATNKWTQMHGLRYKNASYLLGTLQTKAEYNPNFIDYQSFVTYKPSTNW
                     SFSLLGNVSQNVYNFVPESRETTFGTLENLQKFTIYFDGQEKDIFRTGFGAFSTTYQP
                     NKKIGLTFIASAFATNEQENYDIKGEYWLNKVSSASSSSEEVMGVGTYFEHARNEMNV
                     FVCKATHHGTFDINRSHAIVWGVDAVSEKVIDAVNEWEMRDSAGYSLPYSPTEIRLYE
                     SLSSAHTFYSTRLRSYLQYSYKNISHHGLFVLTSGLRCNYWDWNNEMTISPRITASYF
                     PDWQQRINFRFATGVYYQTPSFKEIKDTITKNGNLTHFLNKNIQSQRSLQFIAGTDYF
                     FNWWYRP"
BASE COUNT         4883 a         2111 c         2749 g         4861 t
ORIGIN      
        1 atggctcagc aaggagatgc ttatgctttt aaccttaata aacctcgaat aggcggttat
       61 caatatagtt ttagtggact taaaacatct tttctctatt tagttaggga tgaaatgaaa
      121 aaaaatcctg cgtttattga acaacggaaa accgatttat gtgcttcttt tcaacgtaca
      181 gttgtggaaa ttttaatgga gaagttgctt aaagcatctc aagactatca tatatcaacg
      241 atagctcttg ctggaggtgt atctgctaat agtgcattaa gaaaagcagt cgaaaattat
      301 gctcatatat ataattggac tacctttgtc cccaaattag cttttacaac agataatgct
      361 gcaatggttg ccataacagg gatgtataaa tttaaagcag gcgaatattc agatatatca
      421 ttaagcccat ttgctaaaac attagtataa ttatagtatg caacagtcta ttaaagtaac
      481 acttctgtct tggtgggaaa aattgaaacc catacacggt gtagtttatt ttgtcatctt
      541 attgatttca tgtcattttg catggaaaat ggcatttgtg gaacatacag aaataccacg
      601 taaagttact ttcttgtggt ttgatgttac ccaaattttt tcttttgcag tacataatct
      661 gacagatgtt gtgtattttc ttttgaaaga tattttggat gtgaatatta taaaaattag
      721 taactttttt gtgtatccag acaaagctgt tattgatatt gtttggagtt gtagtggctt
      781 taagcaagcg tacatttttt ttacagtcat agctttttat ccaggaaaac atattcataa
      841 attgtggtac attccacttg gtctagttgt agtatacgtt tttaatgtat ttcgtattgc
      901 cctcattacg tatttattac gtgataattt gggttggttt gatgttttgc acgaaggttc
      961 taaatatgta ttttacgcga ttatcttttt aatatgggta atctgggcgg aaaaatttaa
     1021 caaatcatcc atatagtctc aatgaatcta gtaattgatt gtggtaattc tactactaaa
     1081 atagcggttt ttgatggaga aaatattatt tcaaaatctg ttgttgtaac attacacata
     1141 acggatattg caagtatatt gcatctatat ccgattaaaa atgcggttat ttctaatgtg
     1201 ggtataattc ctactgattg cattgagctg ataaggaaat ctgttgttgt ttatatagac
     1261 cttcagtcaa caacccctat tccactaaaa aataattatg aaacaccatc tacgttaggt
     1321 ttagataggt tagcagttgc agtaggtgca aattatttat atccatcacg caatatatta
     1381 gtgatagatg caggtaccgc catcacatat gagtttatag atgaatataa tacatatcaa
     1441 ggcggaagta tttcgcctgg gttggttact cgttttaaat cgctacatca ttttacacaa
     1501 cgattacctt tggttcagcc tgaaaataca atagaactat ttggaagaaa tactcgtgct
     1561 gctattttaa ctggggtaat aaatggatta gtatatgaaa tagaagggta tattactgat
     1621 tttaagcaaa attatagaga tgttttgatt tttttaacag gaggagacat tttttacttt
     1681 gataaaaaat taaaaagtag catctttgta tccgagaatc tactcctaat aggtttgaat
     1741 cgtattttaa attttaacgc atgtttataa acaaacacat tgcaattata ctgtcgctaa
     1801 cattgataag tatacattta gtagcacaaa gtggtacggt ttcgccctat tcaagatatg
     1861 gttatggcaa cctacgttca ggaggtttta ctgtgtctaa ttcactcggg ggagttgggt
     1921 atgggcttcg ttccaactct atgattaatc cacaaaatcc tgcatcatat accgctgttg
     1981 atagtctttc ttttatgttt gatttcggtg catctgcttc ttttaatcag tttactagtg
     2041 gctttagtca acaacaaaaa tcaacagctc aatttgatta tttagcattg cagattccca
     2101 ttatcaaaaa catgggtatg agccttggat tgatgccgtt ttctacggtt ggttatgcat
     2161 atagtttttc ggaaacctta cagtttaatg ctgacgaatt attgacaaag catacgtttg
     2221 aaggtaatgg aggaatttca aggttgtatg gaggattagc gtatgccata atgccacaat
     2281 tatcattagg cgtaaatgta aattatcttt ttgggaatat cgtacatact agagaactga
     2341 cattcccaga taattctact tactcttcat caaaagaaga atctaatttg tatgtaagta
     2401 cctttttgtg ggatgcggga atacaattta tccaaccggt tggcaaaaaa gatgtcttag
     2461 ttgttggtgg aacgtataca ctcaaaacgc caatgaatat tcgtagtgaa attgttacca
     2521 catctaccca gactattacc gatgatacca actattcatt tgattatcca caatcaatag
     2581 gggtaggtgt aagctacaaa catgggaata gtgtagtatt tgctgctgat tattcttatc
     2641 aatcatttgc tgacgcactt ttttatagcc agagagatac tttgctgaat gcgaggaaat
     2701 tagccattgg ttgtgagtat gttccaaata gtacatctcg ctcttatgtg caaactatac
     2761 gttatcgttt gggagcaaac atagcagagt cctatacaaa agtaaatgaa aaaccatatt
     2821 cagagatggg gattacgtta ggatttgggc ttcctcttaa aaattctaaa tcaattttaa
     2881 atttgaattt ggaatatggt agcagaggta taattaatca aaatttggtg cgtgaggatt
     2941 atgtaaagat tggactggga gtgtctataa atgaaaattg gttttttaag aggaaatttg
     3001 aatagtagct acaataaaaa cagtaggtca gattgaaaaa aaattaaaaa taaaggtatg
     3061 aaagcaaagt taatttttat tggtttagtc gtgttgagtg tattcacggt aaatgctcaa
     3121 aaaggtgttg atacgggaac tcgatatggg ctaggagaag atagtatcag gtgtttagaa
     3181 aacatcagtt tgtatcaaaa ttactatcac attaaagatt ataatacatc ttttcaacat
     3241 tggagagttg tttatacaga atgtccagct tctagtagag atttatacag aataggatct
     3301 attttgttag tatggcaaat gcaaaatgca caagatgtag ataagcaaaa agattatttc
     3361 cgtcaattga tggagaatta cgatcaacgt atcaagtatt ttggtaatga tgcacgtttc
     3421 ccagaatctt gggtaagagg tcgccaagct attgattata ttaattattg ccctgaagat
     3481 cccttaagag agaaagcatt accttggttg aaatattcaa tagatgagcg tcaagcagat
     3541 gcagatgctg acgttattga tggttatatg agagtgttag atggattgta tcgttctgat
     3601 aatgagaaat acagagacat gtatatgacg gagtacttgc gtgtcggaaa aattttggat
     3661 actcgtattg ctagagcttg taaatttgta gataattaca aattagccca aaataatgca
     3721 aatgctttat ttatcgctag tggaattgca aattgtacta cactagagtc tgtttttgca
     3781 tccaaagttc aagagtcaaa atctgatata gaatcactaa ataccattat atcgttattt
     3841 caaagtgcaa aatgtactga atcagaagtt tattttgacg catcatcctt tgctcatgtt
     3901 ataaatccta cggcacaatc agcggcaggt atagcatatc aatctgtgaa aaaagaagac
     3961 ttcttaaaag cagtagatta ttttgaggaa gcaaccaagt tagaaaaagt agatagtgta
     4021 aaatacgatt atcaatatgc tattgcagct atttatgctc aaatgaaacg ttatacacaa
     4081 gcacgtacac atgcattacg tgcagctacc tataatccta aaaaaggaga accatacatt
     4141 ctgcttgcaa tgatgtatgc tgatagtaat atattccctg atgataaaat tttacaaaaa
     4201 actgtctatt gggcagctgt tgataaatta gaaagagcac gttcagtaga tccctcatca
     4261 agagagagag ctcaacaatt aataaataca tatcgacaat attttcctac aaaagaagaa
     4321 attttcttta agccagaatt gcgcgttgga gagtcttttg tagttggagg atggattaat
     4381 gaaacggtta tttgtagaga ataatttcaa tataaaacat atgaaagcat atagcataac
     4441 ggttgttatt ttggcaaccg ttatcctttt tttagaagct tgtacctctt ccattcaaaa
     4501 tgatgcccct aaggtagatc gagagttgat gcctgtattg aaatcgtatg gtgtgtcttc
     4561 agtcgtatct gattctggcg ttacacgttt tcggttcaat acgccagtat ggtatgtata
     4621 cgataaggcg gaagagcctt attgggattt tccagaagga gttcatttcg agcagtttga
     4681 taccgattta aaagtgtatg ctgaagtaga tgccgatact gctatgtatt ataccaacgc
     4741 acaaaagtgg acactttctg gtaatgtaaa agcaaaaaac gttgcgggag aagtatttga
     4801 atctgagcgt atttatattg accaaaaaac tgaaaaaatt tatacagata ggttcattaa
     4861 aataactcaa caacaacgta ttattacagg cataggtttt caatctaacc atactcttac
     4921 acagtataca ataacaaaac ctcaaggtat atttccgata gaacaatgac aacaactgtt
     4981 tttttgatat ttatatctct gcttttctct gcattttttt caggaatgga gattgctttt
     5041 tatgtttcaa ataaactgcg ttttgaaata aataaacaac acagcttact atcttcgtct
     5101 attctttcaa ttttttatgc caatcctagt aaatatattt caatcatttt agttggtaat
     5161 aatattgctc ttgtagtata tggtattcag atggcaaaat ttttagagcc atatttattg
     5221 ttattcttac ataatgactt gttgatagct actttacaaa caattttttc aacaattgtg
     5281 attcttttaa ctggagaatt tttgccaaaa gtgttattta ggcttaatgc ggacctttgg
     5341 ttaaaggttt ttgctatccc gttgttttta tgttatttat ttttatatcc tgtagctacg
     5401 tttgtatatc agatatcaat ttttttattc aaaattctga gagtagatgt ttctaattca
     5461 gcagggggga tgactcttgg acgagcagat ttagataact ttttacaaca aaattatgaa
     5521 aatacaactg acaaaaaaca catagaaaat gaagtcaaat tgtttcaaaa tgccttagat
     5581 ttttctaaaa taaagttgcg agattgcatt attcctcgta cagaaatatt agcggtcgat
     5641 tttgatactg atattgaaga attacgtgta aaatttgttg agtctggtta ttcgcgtata
     5701 ttggtgttta aagaacatat agataatatg gttggttata ttcattcaat tgaattattt
     5761 aaaaaaccac gaaaatggca agatcagata cgcgtgttgc caattgtgcc cgaaacaatg
     5821 gcggctaata agttgatgaa ttatttgttg cagaaaaaga aaagtatggc attggttgtg
     5881 gacgaatttg gcggaacttc tgggatggta actttagagg atattgtaga agaaatattt
     5941 ggtgaaattg aagatgaaca cgatactcaa gattacgtag caaaaaaaat cagtgaaaat
     6001 gaatatgtgt tttctggaag attagaaata gattatgtac aagaacagtt tgatattcaa
     6061 ctccctgttt cagatgagta tttaacaatc gctggtctta tactccatta ttaccaaggg
     6121 tttcctaaga taaatgaaga aataattatt ggtaactata tgtgtaaagt tattaaagct
     6181 tcagctaata aaatagaatt aattaaactg aaagtgttgg caacactata acttgatata
     6241 aaaaaccgaa gaaaatcata tttacactta ctaagtgtaa tattttttgt atattcgcac
     6301 cctaaattat ctaattatta taaagattta taagtataca tcatggcaac attagaaaaa
     6361 atcagacaga aaggtgtttt attaactgtt attattggag gagccttgtt ggcgttcatc
     6421 attggaggta ttgattttaa taccatgaga caaggagcta gagaaacggt tgctgttatc
     6481 aatggggaag aaataaaaat tgctgattac caaaaacgaa ttgatgaaat gactgcattt
     6541 tacaaaatag aattgggtca gtctaacctt tctgaggagt atgtagagca aataaacaac
     6601 gctgtgtgga atacttggag taatgagcaa ttgataggca aacaagctca aagtgtaggt
     6661 ttggttgtta ctgataagga attagcagat gcagtgtatg gagataacat acatccactt
     6721 ttttattcta ttagaatgtt ttataacaac caaggacaat ttgataaaac cttgttattg
     6781 cagtttttaa attcaataag tcaagatacg tcaggtgaag ctgctaaata ttggggattt
     6841 attgaaagaa tgcttaaaaa taatatactt gaagagaaat attatacttt agtgtcaaat
     6901 tcctttaatg cgaatgatat agatacaaaa tatgcttttg atgcacgtac tataactact
     6961 ggattggagt ttgttgctca accatatttt tcgttgtctg actcattgtt ccaagtaaca
     7021 aataaagaaa ttaaagatcg ttataaacga gatcaaatta ggtataccca acaagatgaa
     7081 acgagagata tagctatagt aacatttgat atcaaacctt ctgaacaaga ttttcaagaa
     7141 gtaaaattgt ggatagaaaa attgaaaaat gagttttcta cttctactga ctttgtttca
     7201 atagcgaatc agaattcaga tgttccaacg agtgatattg ctgtaagcag aaataacatt
     7261 gatcctgatt taaaagagtt tgcatttaca gcttcggtgg gtgatgtatt tggtccaata
     7321 tatgtgaatg gtacctacaa aatggctcgt ttgtcggaaa aaggtattct atcgtcagac
     7381 tctgctaagg ttcgtcatat tttaatacaa gagccaaccg ttgaagctac ccaaaaactc
     7441 gttgatagcc tgtttaatgt attaaagggt ggagctgatt ttgcaactgt tgcacaacaa
     7501 tactccaagg caggtacgaa agctcaaggg ggagaacttg gttggattaa ggaaggcgaa
     7561 atagataaag aatttagtac tgctgctatt aatgcaaaag taaatgaatt ttttacactc
     7621 aaattaggaa gtggtatcca tattgtgcaa gtaacagaaa aaactaaacc aatagagaaa
     7681 gtcaaactgg cttctattat tcgtaaagtg gaaccaagct ctcgaacatt tggtgttatt
     7741 tttaaccaag ctagtcaata tattgctcaa aatagaacga tagaaaaatt tgaagctacg
     7801 gctaactctg aaaatggttt atctgttcgt acatatacag tgtctgcaaa tgacaacaaa
     7861 atagcaaata taaaggactc tcgtcaaata atacgatggg cgtacgaaaa aaatgaagga
     7921 gatgttactg acaaagtgtt tgaaagtggg gaacaatttg ttgcagttgc attgaaaagt
     7981 gtaaacccta aagggacaaa atcattatca aaagtaacgg aagaaattaa agctgagttg
     8041 ataaaagaga agaaagcaga taaattagct gctgatttaa caaccaagtt aaaccaagca
     8101 aaaaatctta atgctttagg tttaccattg caagtagtag agagtgtttc ctttgaaacg
     8161 ccattcgttc cgtctatggg cagagagcct aaagtgctag gaaatatcgt ttctcttatc
     8221 aaaacacaac agccaaaagt aattaaagga aatactggtg tatttgttgt gcaagcagtg
     8281 aatgtaggtg ttcaaccagc tttcaataaa aaagaagaaa tgaatttggt tcaacaaaaa
     8341 atgtttagcc aaaacgtatt ggttgaatca ttgaaaagaa cagcagatat tgaagataac
     8401 cgtattcgtt tctactaatc tattctatta tcgaaggtat aaaggcaaac atactttctt
     8461 aataaaagta aaccgaggta gatttatctc ggtttttttt ggtaacaatt tttgaaagat
     8521 gtacttttgt atattaaggt agtatcacta atgttataaa atagtattgt atgagaaaaa
     8581 ttattggttt aattgtatcc ctattattta tttcacaatt tactttttca caaatattga
     8641 ctcctgtaaa gtggactttt gaaatatcag atatagatga ctcttcaaaa gaaatagttg
     8701 ccacggcatc catagatgag cattggaaaa tgtatggaac aaatttacct gaaggaggtc
     8761 caatttctac agtttttata atcgaagcta taacaggagc tagtatagaa ggagattttt
     8821 tatcttctag tcaaccaaaa ttgtattatg atgctatgtt tcaaatggag ttgtcttcgt
     8881 acgaaaaaaa ggcagttttt actcaacgtc tcaaaattat agataaagcc aatttttctg
     8941 tcaaaggata tgtacgatat atggcatgtg atgcaacttc ttgtttgcca cctaccgaag
     9001 catcgttttc gtttggctct gaaccatcac aaacgataac catgcaacat gcaatagaca
     9061 ctcctttttg ggaaccagta attgatgact tacaattagt aggaaatgga gctgttgtac
     9121 aacaacaatc gtattggata atttttgttt ttggatttct tggtggatta ttagccttac
     9181 ttaccccatg tgtatggccc atgataccta tgacagttag tttcttttta aaaagctcta
     9241 aaaataaaaa caaagcaatt aaaaatgctt tgatatatgg tttttctatt atcattattt
     9301 atttaggttt aggtttattg gtaacaggaa tttttggagc aagttcgctg aatagtttag
     9361 ctactagtgc tatctttaat attctatttt ttattcttct aattgtattc gcattgtcat
     9421 tttttggctt atttgaaata acattacctt catcatgggt caatgcaatg gataaaaaag
     9481 tagatactac tactgaattg gttagtattt tctttatggc atttacatta gtacttgttt
     9541 ctttttcttg tacaggtcct attattggta ctcttttggt agaagcggct agtacgggat
     9601 cggtcgttgg gcccgcagta ggtatgtttg gttttgcttt ggcgttggca attccttttg
     9661 ctttcttctc cttgtttcca tcgtatctgc aatctatgcc acgttctggg ggatggttaa
     9721 acatggtaaa ggtaacctta gggtttttag aattagcatt ggcattaaaa ttcttttcag
     9781 tagctgattt ggcttacgga tggggtttgc ttagtaggga tttgtttata ggcatttgga
     9841 ttattatttt tattatttta ggtttgtatt atttgcgtgt tatacgtttt ccaatggata
     9901 aagatgataa aaaaataact cttccacgga ttatctttgc cttggtatct ttcttatttg
     9961 ttgtctattt actgccaggg atgtggggga aacaacgagc ctctataagt gcatttacac
    10021 ctcctatgtc aacacaaata tggaataaaa ataaggatca tgtgcaagct atgtttgaag
    10081 attatgatat agctatgaaa tatgctcaaa ccgttgataa acctgtttta attgattttt
    10141 caggttatgg ttgcgtaaat tgtcgcgaaa tggaagcaac tgtatttgaa gatgatgcaa
    10201 ttaaaaagat gctgaaagaa gattttattt ttgtagtttt gtatgttgat gataaaaaaa
    10261 atcttagcga gccaatgctt gtgtcagaaa ataatgctac gcgtacgttg cgtacacaag
    10321 gagataaatg gagctattta caacgtttaa aatttggagc taatgcccag ccatattatg
    10381 tggttttaaa tgcagcaggc aagccagtag ctccttctta tgcttttaac aaggatgtaa
    10441 aagcttttga aacatattta aagcaagcga ttaagaccta taaaaatgaa aagtaaccat
    10501 gcagaggcta ttattgaagg attaaattct tctcaaaaag aagctgttat gtataataat
    10561 ggaccttcgt tgattattgc tggagcaggg tctggaaaaa cacgtgtgtt aacgtgtaaa
    10621 attgcttatt tattagaatt aggtttgtct cccgaacgaa tagtagctct tacttttacg
    10681 aataaagctg ccagagaaat gaaggaacgc attgcctcta tggtaggtca gaaaatagct
    10741 aaacgtttgg taatggggac ttttcatgct gttttttcac gtatattacg cacagaatca
    10801 gaacatttaa ctataaaaaa agattttgta atatacgata caactgatgc taaaaatact
    10861 atcaaaacca tagtaaaaga aatgcaactt gatgaaaaga tgtataagcc agcattggtg
    10921 ttgtcaagaa tttcttatgc aaaaaataac ttgctatcac cagcagccta tgctacaagt
    10981 aaattaattg ataatgattt ttatgcaaaa attccacgag taaaagatat ttacccagta
    11041 tatgtttccc gtttaaaaca gtcatcagca ttggattttg atgatttatt gtactataca
    11101 aatgtattat ttagagattt ccccaaggta aaagaaaaat atcaagatat ttttgagttt
    11161 attttagtag acgagtacca agatattaat tttgcacaac atttaattgt gaagtttcta
    11221 gccgaaaaac atcatcgtgt gtgcgtagtt ggagacgatg cacaaagcat ttattcgttt
    11281 agaggtgcca atattgaaaa tatacttacg tttaaagata catatcctga aacaagagta
    11341 tttaaacttg agcaaaatta tcgctctaca caaaatattg taggagctgc aaatagtttg
    11401 atttcgaaaa ataaatttaa aataaaaaaa gacgtttttt ccgagaatga accaggagaa
    11461 aaaatacacg tccttacggc ttattcagat attgaggagg gatatattgt tgctaataaa
    11521 atgcaagaat tataccatca aacaactccc aattcgtgga atgattttgt agttttgtat
    11581 cgtacaaact cacaatcgcg tttgattgaa gatgctttga gaaaacaatc tataccgtat
    11641 cgtgtttatg gaggagtgtc gttttaccaa cgagcagaag taaaagacgt gttggcatac
    11701 atacgtttag caattaataa tgacgatgaa gaggcgttta aacgaattgt aaattctcct
    11761 tcacgtggga ttggtaacac aactttggca aaagtatttg ccaaagccca gctttttcaa
    11821 gtttcggcat ttcaggtttc ggctaatcct atgcaatata atgtggaagt aaatgctgga
    11881 actgctacaa agttaattaa ctttaccaat ttaatacacg actttacctt gcaaatgagt
    11941 cagatggatg catatcaagt agcatcatat attactcgaa atgctggaat tattcgtaat
    12001 ttgcaagaat caaaaacagc agaaaatatt agtgcattag aaaatattga tgaactattg
    12061 tctagtatat atgaatatgc tgaacaaaaa aaagaagaag gcgaagaaca tcccagtatt
    12121 gtagagtatt tatcggaaat atccttgttg acagaccaag atacgggtag agatgctgaa
    12181 gaaaataggg taacattaat gacaatacat ttggctaaag ggttggaatt taagaatgtg
    12241 tttattgtag gtgttgaaga agatttactt ccgtctgtgt atgctgacga tgaaaaagga
    12301 ttggaagaag agaggcgttt attttacgtt gctattacta gagccgaaaa aagatgtttt
    12361 atctcttatt caaaaagtcg ctttagaaat ggtcaaatga cttatgctaa acatagtcga
    12421 tttatttctg atatagattc tcattttttg catttacccg ttgacttttc ttctaagaat
    12481 acaagtcatt ttgatttttc gcaacaaaaa gagtctttct ttagttctaa taagccattg
    12541 acacaaactg taataccaaa acctacatac aataaaaact taactaagtt gtctgctatc
    12601 agcaataaaa ctttatcaca gcctgtttta tctgaggtta aaataggctc acgtatcgaa
    12661 catgaacgat ttggaaaagg tgttataata gccattactg gagatgttgc agatacacgt
    12721 ataactgtga agtttgataa tgctggagaa agggttttat tacttaaatt tgcgaaattt
    12781 tcattatgct aattctttat tatttatgct aatttcgtgt agacatatca tctttttgct
    12841 tatcatttgt acaaacatac tacatgtatt tgcaaatgaa aatatggtag tgagaggatt
    12901 tgtactgaat gaacagcaac agcctattga aatggttgat gtttctgttc agcaatcgcc
    12961 tacaagaacc gttacagata aatttggaaa atttgagatt catgtttcag cccaagattc
    13021 tttcttttta gttttttcct ccattgcaca tcgtcccgct atacgaaagg tatatgtaaa
    13081 tgaaaaatac gttaccaatg taactgtagt gttgcaagat gtaataaatg aattaacgga
    13141 tattgaagta aaaggagaaa agcatcagca tactacagca gaatgggtaa atactgaatt
    13201 tatcaaagtc ttgccatctg cctcaggcaa aagcattgaa agtttgatta aatcattttc
    13261 tggagttagt tcaaccaatg agctgagttc tcagtattca gttagaggtg gtagttatga
    13321 tgaaaatagt gtttatgtta atggtataga gatttaccgt ccttttctta tccgttcagg
    13381 agagcaagaa gggttaagtt ttatcaacac ggatatggtt ggggaggtaa ctttttcagc
    13441 tggtggattt gacgctagat atggagataa attatcctct gtgcttgata ttacatataa
    13501 acaaccttcc acttttgaag gaaatgttgc tgtcagcttt gctggggcat ctgcttatat
    13561 tggttcagca actaataaat ggactcaaat gcatggctta cgctataaaa atgcttctta
    13621 tttgcttgga acgttgcaaa ctaaagcaga gtataatcct aattttattg attatcagtc
    13681 ttttgttaca tataaaccca gtaccaattg gtctttctct ctattaggca atgtatccca
    13741 aaatgtgtat aattttgtac ccgaatcaag agaaacaact tttgggacct tagaaaactt
    13801 gcaaaaattt accatctatt ttgatggaca agaaaaagat atttttcgta caggttttgg
    13861 agcgttttca acaacctatc agcccaataa aaaaatcggg ttaactttta ttgcttcagc
    13921 atttgcaacc aacgagcaag aaaattatga tattaaaggc gagtattggc ttaataaagt
    13981 ttcgtctgcg agtagtagtt cggaggaggt aatgggggta ggtacatact ttgaacatgc
    14041 acgtaatgaa atgaatgtgt ttgtttgtaa ggctactcat catggaactt ttgatataaa
    14101 tcgttcccat gccattgtgt ggggagtaga tgctgtatcc gaaaaagtaa tagatgctgt
    14161 taatgagtgg gaaatgaggg attctgcggg gtattcattg ccatatagcc ccactgaaat
    14221 aaggctatat gaaagcttat cttctgcaca cactttttat tccaccagat tacgttctta
    14281 tttgcagtat agttataaaa atatttctca tcatggttta tttgtgctaa cctcaggttt
    14341 gcgatgtaat tattgggatt ggaataatga aatgaccatt agccctcgta tcaccgcatc
    14401 gtatttccct gattggcaac aacggataaa ttttagattt gccacaggag tgtattatca
    14461 aacgccttct tttaaagaaa ttaaagatac aattacgaaa aatggtaatt taactcattt
    14521 tttaaataaa aatattcaat ctcaacgttc gttacaattt attgcaggta ccgattattt
    14581 tttcaattgg tggtacagac cctt
//
LOCUS       DAOSRP010000038        14582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4210_length_14582_cov_6.306670, whole genome shotgun sequence.
ACCESSION   DAOSRP010000038 DAOSRP010000000
VERSION     DAOSRP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_14582_cov_6.306670"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1794)
                     /locus_tag="PLJ82_03665"
     CDS             complement(328..1794)
                     /locus_tag="PLJ82_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40636.1"
                     /translation="MKTILHKLLIASWLIISPTIAFGRFTTLPTIPEHPSIIGFHIPN
                     NPSAPSGLRNLSLEPKQQLDSIVVAGKEKIVYLYNDNGLHIMLTQYKWNGVSWRPEIQ
                     VDLLYNESNKVATSINYTWNIVLGEWTPQWKTVYTYNVYGQTLTEQYITYDRNTKKWE
                     NIKRYAYTYTNAGVITKSTHANWNTITNTWIVDWTTDYVYDIKKQRIADVSYSEGYVP
                     ASKTEYTYNANGALVRKNDFYSNNTTNRWEPSAKEEYLLDNQLRPVVKTYSFWNSILQ
                     VWENLYKSNVEFDNNGNSLSIINYKWNVEHLRWDITDKQEAQVNASGDITALHYFDWN
                     TATNNWIDRSTHEWNYNDNVTLTMLVLPNYYTNFTNSKHQVTSLVSSYNNNGNEETEI
                     QTYYYSSGNNTTHIMENDTDAFVKVYYNPTNEYIEVVSDAENNIRFYLYDSKGSMITQ
                     TTLQEGSGKISISQLSPGTYLYTLLNNNEPLRGKLIKQ"
     gene            2329..2410
                     /locus_tag="PLJ82_03670"
     tRNA            2329..2410
                     /locus_tag="PLJ82_03670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2363..2365,aa:Leu,seq:tag)
     gene            2506..3858
                     /gene="tig"
                     /locus_tag="PLJ82_03675"
     CDS             2506..3858
                     /gene="tig"
                     /locus_tag="PLJ82_03675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOR40637.1"
                     /translation="MNVSQNNIDQVNAVLSIQVKKADYTEKVEKSLKDYRKKANMPGF
                     RPGTVPLGLIKKMYGKAILAEEINKLVSESLYDHIQKNNIPVLGEPLPSENAPVPNFD
                     TQEDFDFSFDIALAPEINLNLTTKNKVTLYNIAVSDEMVAKQVDAYKGRFGKHAPADK
                     VEEKDVVKGELVELLPNGDINENGIKVENALLSPAYMKDKKEQAKVIGLALESQFRFN
                     PAKAFESETEIASLLQVSKEIAADCKADFLFTIKDITRYVEAEMNQDLFDAALGKDVV
                     SSEAEFIDKIKESLKAQFAVDSEFKLSIDMKELVVKQLEGLAFPDVFLKRWLKVSNEN
                     LTEDAIEQEYPKMIEDLKWQLAKDKVAKENELKLEKEDVEAVAKQAAKAQFAQYGMMN
                     VPDDVLDGYVKDMLKNKDAAKNITERAVEQKTIDALKTKVTIVSKEISFEDFNKLFEN
                     "
     gene            4005..4673
                     /gene="clpP"
                     /locus_tag="PLJ82_03680"
     CDS             4005..4673
                     /gene="clpP"
                     /locus_tag="PLJ82_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOR40638.1"
                     /translation="MNTEFKKYATKHLGMNGLALDKYVDITSSYISPTIIEERQLNVA
                     QMDVFSRLMMDRIIFLGTQVDDYTANVIQAQLLYLDSADPGKDISIYLNTPGGSVYAG
                     LGIYDTMQFISSNVATICTGMAASMGAVLLTAGTAGKRSALKHSRIMIHQPMGGAQGQ
                     ASDIEITAREIQKLKKELYTIIAEHSGNTYERIEQDSDRDYWMTAEEAKAYGMIDEVL
                     VKQK"
     gene            4694..5914
                     /gene="clpX"
                     /locus_tag="PLJ82_03685"
     CDS             4694..5914
                     /gene="clpX"
                     /locus_tag="PLJ82_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOR40639.1"
                     /translation="MSKKDDKCSFCGRGRQEAPLLMQGQTGNICNDCAEQAATIAKET
                     FSKQTDKYQLDKQTLPKPQELKAFVDQYVIGQDDAKKNLCVAVYNHYKRLLQKNTHDD
                     VEIEKSNIILVGATGTGKTLLARTIAKKLHVPFTIVDATVLTEAGYVGEDIESILTRL
                     LQVADYDVKAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSIVNVP
                     PQGGRKHPDQKMIAVNTQNILFICGGAFDGIEKKIAARLNTQIVGYAAAKITHQIDKD
                     NLLQYISPQDLKAFGLIPEIIGRLPILTYLNPLDREALRLILTEPKNSIIKQYKKLFA
                     MDDVVLEFDKDVLDYIVDKSIEYKLGARGLRSIAEMIMVDYMYDIPSSNEHQLRIPLA
                     IAKEKVEKGRMTPV"
     gene            6020..8212
                     /gene="recQ"
                     /locus_tag="PLJ82_03690"
     CDS             6020..8212
                     /gene="recQ"
                     /locus_tag="PLJ82_03690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOR40640.1"
                     /translation="MSTSLELTQELKKNFGFDSFKGNQEAIIKNLLAGNDTFVLMPTG
                     GGKSLCYQLPSLMMEGTAIVISPLIALMKNQVDAMKAFSEEDGVAHFLNSSLSKAAIE
                     DVKNDVISGKTKLLYVAPESLTKEENILFLKKVKISFYAVDEAHCISEWGHDFRPEYR
                     RIRPLINEIGSAPVVALTATATPKVQHDIQKNLGMVNATVFKSSFNRENLYYEVRTKN
                     QHIDKEIIKYIKSQHGKSGIIYCLSRKKVEELAETLCLNGINALPYHAGMESAVRTAN
                     QDSFLMEKVDVIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGQC
                     ITFYSNKDLQKLEKFMQGKPISEQEIGKQLLLETAAYAESSVCRRTLLLHYFGEEYTQ
                     DNCGSCDNCLNPKKQMEAQDLLCTVLETILSVKEKFKAEHIVDVIVGKETAEVIDYKH
                     QDLEVYGTGESEKPTVWHAVIRQGMLSGYIDKEIENYGLLKVTKSGRAFLEKPTSFKI
                     TKDNDFEEEDDDDDATIRRSGGGTGAADEVLFSMLKDLRKKMSKRLEVPPFVIFQDPS
                     LEAMATTYPVTMEELQNIPGVGAGKAKRYGEDFLRLIKTHVQENEIERPVDLRVRTVA
                     NKSKLKVFIIQAIDRKIALDDIAISKGLEFPELLDEIEAIIYSGTKINIDYFINEVME
                     EDHILDIYEYFRESQTDEVETALAELGSDYTEEEIRLIRIKFVSEIGN"
     gene            complement(8337..10637)
                     /locus_tag="PLJ82_03695"
     CDS             complement(8337..10637)
                     /locus_tag="PLJ82_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40641.1"
                     /translation="MKKIILFSFFIFSFCAILSAQIPASKVTDAHIVGDVTDAHSGDH
                     LPYVNVVIKGTTIGIMTDASGHYFLKNLPIGTFTIIYSMVGYQTVERTITIEPNKTLE
                     LKIKLEETSIMMDNIVVTGNKYETKKKETSSIVNVVTPLIFTSTSANSIGEALDYQTG
                     LRTEMTCGNCGVPQLRINGLEGHYSQLLLDSRPLFSSLASVYGLEQMPTGMIDRVEVI
                     RGGASALYGSNAIGGVVNIITKEPTRNFVELSNNTALMGNTAIDANTTFNASIIGNNS
                     NVGVFLFGVLRDRQAYDRDNDGFSEMPALQGSVVGFRSYFKTSNYSKITTEYHHSTEY
                     RRGGDLLDLPAHEANLAEQLQHNIDAGSVKFDLYTKDNKHFISTYSAFQHIVRNSYFG
                     TGQDLDAYGKSKDINSVTGAQYRYSMDNLLFMPADFSMGIEYTYNQLQDQMLGHGIDV
                     NQNVSIFGGYLQNEWKNNQLSLLAGARVEKHNLMNNPVVSPRVSLRYNPTHNIVLRTG
                     YASGYRAPQIYNEDLHVAAVGGEVSIISQDANLRPEYSHSFSVSADAYHRFGFWETNL
                     TVDGFYTKLNDVFALEENGFDSKGNLLLLRVNESGATVTGVNIEGVLGYKNLLSLQLG
                     YTLQSSLYDEPVTWSTNTAIEATRRMQRTPDHYGYAVMNYNATKNFTASVSGKYTGSM
                     LVPHFAGYIAEDTETISSPFWDMGVKLAYKIPFYKYYALEVNGGVKNILDSFQPDLDK
                     GMDKDAGYIYGPTLPRTFFVGLSLKL"
     gene            10971..11108
                     /locus_tag="PLJ82_03700"
     CDS             10971..11108
                     /locus_tag="PLJ82_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40642.1"
                     /translation="MNIPYPISLSMRVGLGREWMRVCNNGIINNMRMRKQGRTYPVSN
                     K"
     gene            complement(11225..11869)
                     /locus_tag="PLJ82_03705"
     CDS             complement(11225..11869)
                     /locus_tag="PLJ82_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOR40643.1"
                     /translation="MLIEQVPQCIRRFFQPVIWRGNAKEKKIFLTFDDGPTPEITPWI
                     LDLLDTYNVKATFFCIGNNVEKYPDLFLEIQQRGHQVGNHAYTHKRGMFTDTKIFFED
                     IARAQKLINSDLFRPPHGHITPRQIKKLKHTFKIVLWDVITRDYDATLPPEIILQNVK
                     KYSRNGSIIVFHDSLKAEKNLRYALPKSLAFWKEKGYECSLIHPTITTSSDKNR"
     gene            complement(11878..>14582)
                     /locus_tag="PLJ82_03710"
     CDS             complement(11878..>14582)
                     /locus_tag="PLJ82_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40644.1"
                     /translation="SIGVHLLNLLTIPALVLVYYFRKTEKVSLKGGTWALLISFAILG
                     FIMYGFIPQFIANAGIAELFFVNTLGFSFNTGAFFYYFTTIAVLVWAIIELYSKKPSA
                     TRIKVSFIGSLILLGIPFLLGSLLLGLVLLSILIFYFIKLKEKISIRLLSTIIISLTM
                     MLIGYSSYALIVIRSAANPPMDQNSPDNIFALQSYLNREQYGDRPLFYGAYYSAEPKL
                     IEEDGYCKPSVKEGAKTWGQAEKTSPNEKDRYIVTGTKFELEYDSQFKTIFPRLHSSN
                     ESHIKAYNSWITIQGKTVKYDKCGRIIDVVVPTFGENLRFFFSYQLNYMYWRYFLWNF
                     SGRQNDLQGSGEVTRGNWITGFNFIDNYLVGDQTNLPPDLAENKAHNAYFMLPLLLGI
                     IGIIYQLMWSNKKGNQAFWVIGWLFFMTGIAIVMYLNQGPSEPRERDYAYAGSFYAFS
                     IWIGFGVAGIAKWLESKKINPTLAAIIALLLAAPAPIIMAQQNWDDHDRSNRYVVRDF
                     AQNYFNSLAPNAIIFTNGDNDTFPLWYTQEVEGTAVSLDKRVCNLSYLNTDWYIDQMK
                     RPAYASAPLPISWGRETYGEGKRNYAYAYNLMTGTVDLKDALHFLASDDPRTKQDPRT
                     GDTRDFIPATTLSLAVNADNLVKQGAISADEKGSVLPAIEFKLRDRQLLTKSELMILE
                     MLAQNNWERPIYYAVTVGSEMHLGLRKYFQLEGLAYRIVPRTDSIPTGTVHTDMMYDN
                     MMNKFKFGNIADPSVYLDENILRMSKVQRQMFSSLVGALLEEGDSIRALKALDYCMKV
                     VPGTTVPHDFSSTSLAEYYYMLGEKEKATDLINTIGNRSIAYLEWISKLSPQKKASAA
                     YDAHIQLSSLRNIAYITQNYDEKTFEKYYASLMQYASIFDHLLR"
BASE COUNT         4762 a         2356 c         2736 g         4728 t
ORIGIN      
        1 aacaagactt actagaagta aaattataga ttctaccaac aataatgctc caataactcg
       61 tacaaccaat cgaaaattaa attgagtatt cattatccaa cgattaataa agagtgttta
      121 ttttaccatt tttttgacaa aaaggggtct ttggaaatgc tagagagaat gcacaatgta
      181 tttctctgag agcataaaga aaggctgaca aagatattcg ttttacatta tctgtacaat
      241 aaaaattgaa cttaatttgg tcttttgaca aaaagaaaaa caagcatgct ggtatgcctg
      301 tttttcttgt ataaattaca gtgtttttta ctgttttatc aatttaccac gtaagggttc
      361 gttgttgttc agtaaggtgt atagatacgt tcctggtgat aattgagata tagatatttt
      421 ccctgaacct tcttgtaaag tcgtttgagt aatcatagat cctttgctat cgtataagta
      481 gaatcgtata ttgttttcag catcactcac cacttctata tactcattag ttggattgta
      541 atagactttt acgaatgcgt ctgtatcgtt ttccatgatg tgtgttgtgt tgttgccaga
      601 cgaataataa tacgtttgaa tctcagtttc ctcgtttcca ttgttattat aggatgatac
      661 taacgacgtt acttggtgtt tgctattcgt aaaattggta tagtaattgg gtaacactaa
      721 catggttaag gttacattgt cattgtaatt ccattcatgc gtacttctat ctatccaatt
      781 gttggttgct gtattccaat caaaataatg taatgccgta atatccccac tggcatttac
      841 ctgagcttct tgtttatcag taatatccca acgcaaatgc tcaacattcc atttataatt
      901 aatgattgat aagctatttc cattgttatc aaattcaaca ttgcttttat ataggttttc
      961 ccatacttgc aatatgctgt tccaaaaact atatgttttt acaactggtc gtagttgatt
     1021 atccaatagg tattcctctt ttgccgatgg ttcccatcta ttggtggtgt tatttgaata
     1081 aaaatcattt ttgcgtacta aagctccatt tgcattgtat gtgtactctg ttttagaagc
     1141 gggtacatat ccttcgctgt acgacacatc tgcaatacgt tgttttttta tatcgtacac
     1201 gtaatccgtt gtccagtcta ctatccaggt atttgttatt gtgttccaat tagcatgagt
     1261 gctttttgtt ataacacctg cattggtata ggtataagca tatcttttaa tgttttccca
     1321 tttttttgtg ttacggtcat atgtgatgta ttgttctgta agtgtttgtc catatacgtt
     1381 atatgtatat actgttttcc attggggagt ccattctccc aacacaatat tccacgtata
     1441 attgatagat gtagctactt tattcgattc attatacagc aaatctactt gtatttctgg
     1501 tcgccaagac actccattcc atttatattg tgtaagcatg atatgtaatc cattgtcatt
     1561 atataggtat acaatttttt cttttcccgc tacaacaata ctgtctagtt gttgtttggg
     1621 ttcaagagaa agattgcgta atcccgatgg tgcagaaggg ttgtttggaa tgtgaaaccc
     1681 aattatactt ggatgttcag gtatagtagg taaggtagta aaacgaccaa acgcaattgt
     1741 aggcgaaata ataagccaac ttgcaattag taatttatgt agtattgttt tcatgatatt
     1801 catttttagt ttttttgctt aatctaacaa attataattt gtatagtatt atcggcgaaa
     1861 ttctgataca aatgtaatag gtatactatc agaccagaat catttacacg tatttttaca
     1921 tgttatttaa cataaataca gtaagatttt acaatcttat tacaaaataa atgaatatgt
     1981 aaacaaaaca tcctaaaaaa acaaaaggta gtctcgcctg actacctact tgttaacctt
     2041 aaaatcaaat actatgaaaa aatcatactg cgaaagtaag gtgtttacta aactattcaa
     2101 aaaaatgaac ccataaatcc gtatttttac ccttattaag tgctgaatgt tacaaattta
     2161 ttacaaaaca gtcgtttcat tgttcaattt ttttgtttcc ttgctgtagc tattagatgg
     2221 ggaaataata aaagaggcta tttttttaag taaaatgcac tcaaaataaa gaaaatcagt
     2281 gaatttgttt ttgttttgaa aatattttta tacttttgca tcgcttttgc ggctgtggcg
     2341 taattggtag ccgcgccaga cttaggatct ggtgccgaga ggcgtggggg ttcgagtccc
     2401 ttcagccgca caacaaaaaa gaaccgttgt cttagcaaac acaacggttt tttttgccta
     2461 taacaaaaag tattttgctg aataataaat taatttcgta gtacaatgaa tgtttcacaa
     2521 aacaacatcg accaagtaaa tgccgtactt tcaattcaag taaaaaaagc ggattatact
     2581 gaaaaagtag aaaaatcatt aaaagattat cgtaaaaaag caaatatgcc tgggtttaga
     2641 ccaggaactg tgcctcttgg attaatcaaa aagatgtacg gaaaagctat tttggcagaa
     2701 gaaattaata aacttgtttc agaatcgcta tacgatcata tccaaaagaa taatatacct
     2761 gttttgggag agcctcttcc atcggaaaac gcccctgtgc caaattttga cacacaagag
     2821 gatttcgatt tttcttttga tattgctctg gctcctgaaa tcaaccttaa tttaacaacc
     2881 aaaaataagg taacattata caatattgct gtaagtgacg aaatggttgc caaacaagta
     2941 gatgcttaca aaggacgttt tggaaaacac gctccagcgg ataaggtaga agaaaaagat
     3001 gtagtaaaag gcgaattagt tgagttattg cctaatggcg atatcaacga aaatggtatt
     3061 aaagtagaaa atgctctatt aagtcctgcg tatatgaaag acaaaaaaga gcaagctaaa
     3121 gttattggat tagctcttga gtcgcaattt aggtttaatc cagctaaagc atttgaaagc
     3181 gaaacagaaa tagcctcttt attgcaagtt agtaaagaaa ttgctgccga ttgcaaagct
     3241 gatttcttgt ttaccatcaa agatattaca cgctatgtcg aggctgaaat gaatcaagat
     3301 ttatttgatg cagctcttgg aaaagatgtg gtatcttctg aggctgaatt tattgataaa
     3361 attaaagaaa gcttaaaagc tcaatttgct gttgatagtg agtttaaact gtctattgac
     3421 atgaaggagt tggtggttaa acaacttgaa ggcttagctt ttcctgatgt attcttaaaa
     3481 agatggttga aagtgagcaa cgaaaacctg accgaagacg ctattgaaca agaatatcct
     3541 aaaatgatag aagatttgaa atggcaatta gcaaaggata aagtggcgaa agaaaacgaa
     3601 ttgaaattag aaaaagaaga cgtagaagca gtagccaaac aagcagctaa agctcaattt
     3661 gctcaatatg gcatgatgaa tgtgcctgat gatgtacttg atggctatgt aaaagatatg
     3721 cttaaaaaca aagatgctgc aaaaaatatt accgaacgtg ccgtagaaca aaaaacaatt
     3781 gatgccctaa aaacaaaagt aacaattgtt tcaaaagaaa tatcattcga agattttaac
     3841 aaactgtttg aaaattaagc atataggata taaaaaatac taaaaaaact ttcttttaaa
     3901 tttgggagtg taatttgttt gttttaaaac aaaaaaaaat tatacctttg tttccctgat
     3961 ttaaaagaaa gtttttttat gtaccatata atttggaaaa caatatgaat acagaattta
     4021 aaaaatatgc aacaaagcat ctcggaatga acggattggc tttggataaa tatgtggata
     4081 ttacaagtag ctatatatcg ccaactatta ttgaagaaag acaactcaat gtcgcacaaa
     4141 tggatgtttt ttcacgtttg atgatggaca gaattatctt tttaggaaca caagtggacg
     4201 attatactgc caatgtaatt caggctcaat tgttgtatct tgattctgcc gatccaggga
     4261 aagatatttc tatttatctt aatacacctg gaggttctgt gtatgcaggc ttaggtatat
     4321 acgataccat gcagtttatt tctagcaatg tagctaccat ttgtacaggc atggcagcct
     4381 ctatgggtgc tgtactactt accgctggta ctgcaggcaa acgttcagca ttaaagcact
     4441 cccgtattat gattcaccaa ccaatgggtg gagctcaagg tcaagcttcg gatattgaaa
     4501 ttactgcccg tgaaattcaa aaactcaaaa aagaattata caccattatt gccgaacatt
     4561 cgggcaatac atatgaacgt atcgaacagg actctgaccg tgactattgg atgacggcag
     4621 aagaagcaaa agcctatggt atgattgacg aagtgttggt aaaacaaaaa taagagcaag
     4681 taggtataaa gttatgtcaa agaaagacga taaatgtagt ttttgtggac gaggacgtca
     4741 ggaagcgcct ctactgatgc aaggacaaac aggaaatata tgcaacgatt gtgccgaaca
     4801 agctgctacc attgccaaag agactttttc taaacaaacg gataagtatc aactcgataa
     4861 acaaaccttg cctaaaccac aagaattaaa ggcatttgtt gatcagtatg taattggaca
     4921 agatgatgca aaaaagaatc tatgtgtagc cgtttacaat cactataaac gattgttaca
     4981 aaaaaacaca catgatgatg tagagattga aaaatcgaac atcattttag ttggagctac
     5041 aggcacagga aaaaccttgt tagcacgcac tattgctaaa aaactgcatg tcccttttac
     5101 tattgtagat gcaactgttt taaccgaagc agggtatgtt ggtgaagata tcgaaagtat
     5161 cttaactcgc ctattgcaag tagctgacta tgatgtgaaa gctgctgaaa aaggtatcgt
     5221 atttattgac gaaatagata aaattgctcg caaaggagat aacccctcta ttactcgcga
     5281 tgtgagtggt gagggcgtac aacaaggatt actaaagctc cttgaaggtt ctattgtaaa
     5341 cgtacctcct caaggaggac gaaaacatcc tgaccaaaaa atgattgccg taaatactca
     5401 aaatatttta tttatttgtg ggggagcatt tgatggtatt gagaaaaaaa ttgcagcacg
     5461 attaaatact caaatagtgg gatatgcggc tgcaaaaata acgcatcaaa tagataaaga
     5521 taatttactt caatatattt caccacaaga cttaaaagct tttggattga tacccgaaat
     5581 aattggaaga ttacctattt taacgtatct caatccatta gatagagaag cattgagatt
     5641 aatattaacc gaaccaaaga attctattat caagcaatat aaaaaattgt ttgctatgga
     5701 tgatgttgta ttagagtttg acaaagatgt tcttgattat attgtagata aatctattga
     5761 atataagctg ggtgctagag gtttacgctc tatcgctgag atgattatgg tagattatat
     5821 gtatgatata ccttcatcaa atgaacatca attacgcatt cctttggcaa ttgccaaaga
     5881 gaaagtggag aaaggaagaa tgacaccagt gtaatttatt caaaattttt ctcgctttca
     5941 tttgattatt aaaaacaagt tattacattt gttttattat acaaacaaga gcaactagtt
     6001 ttaatcttac attattgtaa tgtcaacatc tttagaactt acacaagaat taaaaaagaa
     6061 ttttggattc gattctttta aagggaatca agaagccatt attaaaaatc tgttagcagg
     6121 taatgataca tttgtattga tgcctactgg gggaggtaag tctttgtgtt accagttacc
     6181 gtcgcttatg atggaaggaa cagctatcgt tatttctcca cttatcgctt tgatgaaaaa
     6241 tcaagtagat gcaatgaaag catttagcga agaagatggc gtggctcatt ttttaaattc
     6301 atcactatcg aaagcagcta tcgaagatgt gaaaaatgat gttatttctg gcaaaacgaa
     6361 attgttgtat gtagcccctg aatctcttac caaagaagaa aatattttat ttctgaaaaa
     6421 agtaaaaatt tctttttatg cagttgatga ggctcattgt atttcggaat gggggcacga
     6481 ttttagacca gaataccgca gaattcgtcc gttaattaat gaaataggtt cagctccagt
     6541 ggtagctctt actgcaacgg ctacacccaa agtgcaacat gatatacaaa aaaatttagg
     6601 aatggtaaat gctactgttt ttaaatcgtc ttttaataga gaaaatttgt attatgaggt
     6661 acgcacaaag aatcagcata ttgataaaga aattattaaa tatatcaaat cgcaacatgg
     6721 taagtcgggt attatttatt gtttaagtcg taagaaagta gaagaattag ctgaaactct
     6781 ctgtttaaat ggaataaatg ccttgccata ccatgcggga atggaatctg ctgttcgtac
     6841 agccaatcaa gatagttttt tgatggaaaa agttgatgta attgtggcaa caattgcatt
     6901 tggaatgggt attgataagc ccgatgttcg ttttgtaata cattatgata tacccaaaag
     6961 cctcgaaggt tattaccaag aaacaggtag agctggtaga gatggaggtg aaggtcaatg
     7021 cattacgttt tactcaaata aagacttgca gaaattagaa aaatttatgc aaggaaaacc
     7081 tatatcagaa caagaaattg gcaaacaact cttgttagaa acggcagctt atgctgagtc
     7141 gtctgtatgt agaaggacat tattgttaca ttatttcgga gaagaataca cacaagacaa
     7201 ttgtggtagt tgtgataatt gtttgaaccc taagaagcaa atggaagcac aggatttatt
     7261 atgtacagtt ttggaaacga tcctttcagt gaaagaaaaa tttaaggcag agcatattgt
     7321 agatgtaatc gttgggaaag aaaccgcgga agtgattgat tataaacacc aagatttgga
     7381 agtatatggt acaggcgaaa gcgaaaagcc gactgtttgg catgctgtta tacgacaagg
     7441 tatgttatct ggctatatcg acaaagagat agaaaattat ggattgttga aggttaccaa
     7501 aagtggtcgt gcttttttag aaaaacctac aagctttaag ataacaaaag ataatgattt
     7561 tgaggaagaa gatgatgatg atgatgcaac cataagaagg tcgggtggag gcactggagc
     7621 tgctgatgag gtattgtttt ctatgctcaa agacttacga aagaagatgt cgaagagatt
     7681 ggaagttccg ccttttgtca ttttccaaga cccttcgttg gaggcaatgg ctacaacgta
     7741 tccagttact atggaagagt tgcagaatat accaggagta ggtgcaggta aagccaaacg
     7801 ctatggagaa gattttttgc gtttgattaa aacacatgtg caagaaaatg aaattgaacg
     7861 ccctgttgat ttacgggtgc gtacagtggc taataaatct aaactaaaag tctttatcat
     7921 tcaagctatt gacagaaaaa ttgcattaga tgatatcgct atatccaagg ggttagagtt
     7981 tcctgagttg ttggatgaga ttgaagccat catttattcg ggtactaaaa tcaatatcga
     8041 ttattttatc aacgaggtaa tggaggaaga tcatatattg gatatatacg agtattttag
     8101 agagtctcaa acagacgaag tggaaacagc tcttgccgaa ttgggaagcg attatacaga
     8161 agaagaaatt cgattgatac gcattaagtt tgtgtctgaa ataggcaatt aacttcttgc
     8221 agaaataaga agtttaaaga gatagcattt atttgttgtc tctttttttt tgtaaaaaaa
     8281 tgggacaacg aaaagttgtc ccacaaaaga aaaggctcac tactttattt tcttttttat
     8341 agttttagac ttagtcctac gaaaaatgta cgaggcaatg taggtccgta aatatatcca
     8401 gcatctttat ccataccttt atctaagtca ggctgaaagc tatccagtat attttttaca
     8461 cctccgttta cttcaagtgc atagtatttg taaaaaggta ttttgtatgc tagtttaacc
     8521 cccatatccc aaaatggaga tgaaattgtt tcagtatctt cggctatgta acctgcaaaa
     8581 tgtggtacta gcatacttcc tgtatattta ccagatactg atgctgtaaa gtttttagtt
     8641 gcgttataat tcataacagc gtaaccataa tggtctggtg tgcgttgcat gcgtcttgtg
     8701 gcttcgatag cagtattggt tgaccaggtt actggttcat catataagct actttgtaag
     8761 gtatagccta attgtagtga tagtaagttt ttatatccta acactccttc gatatttact
     8821 cccgttacgg ttgctcccga ttcattaacc cgcaacaata ataagttacc tttgctatca
     8881 aaaccatttt cttctagtgc aaatacatca ttcagttttg tatagaagcc atctactgtt
     8941 aagttggttt cccaaaaacc aaaacgatgg tatgcatctg ctgatacaga gaaactatgc
     9001 gaatattcag gacgaaggtt tgcgtcttgt gagataatag acacttctcc acccacagcc
     9061 gctacgtgta aatcttcgtt gtatatttgt ggagcacggt atccgctagc gtatccagtt
     9121 cttagtacaa tgttgtgtgt tgggttgtag cgtaaactta cacggggcga taccactgga
     9181 ttgttcatta aattgtgttt ttctacacga gcacctgcca ataggcttag ttgattattc
     9241 ttccactcgt tttgaaggta tccaccaaag atgctaacat tttggtttac atcaattccg
     9301 tgccctaaca tttggtcttg aagttgatta tatgtatatt caattcccat actaaaatct
     9361 gctggcataa acaatagatt atccatcgaa tagcggtatt gggctcctgt tacagaatta
     9421 atatcctttg attttccata ggcgtcaagg tcttgacctg ttccaaaata actattacgc
     9481 acaatatgtt gaaatgcact ataggtagaa ataaagtgtt tgttatcttt ggtatacaaa
     9541 tcaaatttaa cgctacctgc atctatgtta tgttgaagtt gctctgctaa attggcttca
     9601 tgggctggta aatcgagcaa gtcgcctcct cttctgtatt cagttgaatg gtggtattcg
     9661 gtagtaattt ttgaataatt actagtttta aagtacgagc gaaagcctac tacagaacct
     9721 tgcaatgcgg gcatttcaga aaacccatca ttgtctctat cgtatgcctg tctgtcgcgt
     9781 aatactccaa ataaaaatac gcctacgttg gaattgttac cgataattga ggcattgaac
     9841 gtagtattgg catctattgc agtattaccc atgagtgcag tattattgga tagctcaaca
     9901 aaattgcgtg taggttcttt ggtaatgata tttactacac ctccgattgc atttgaccca
     9961 tataatgcag aagctccacc tctaataact tctactctat ctatcattcc agttggcatt
    10021 tgttctagtc cgtataccga agccaacgag ctaaataatg ggcgactgtc taataataat
    10081 tgtgaataat gaccttctaa tccattaata cgcaattgcg gaacgccaca attaccacat
    10141 gtcatttcag tacgtaaccc agtttgataa tctaaagctt cgcctatact gtttgccgaa
    10201 gtacttgtga aaataagtgg ggtaacgaca tttacaatag acgatgtttc ttttttcttt
    10261 gtttcatatt tattccctgt tactacaata ttatccatca taatggatgt ttcttccagt
    10321 ttaattttta actcaagtgt tttgttgggt tctattgtta ttgttctttc aacggtttgg
    10381 tatccaacca tggaatatat gatggtaaag gttccgatag gtagattttt tagaaaataa
    10441 tgacccgaag cgtcagtcat tataccaatg gtagttcctt taattactac gttaacatag
    10501 ggtaaatgat cgccagagtg agcatctgta acgtccccta caatatgtgc atcggttact
    10561 ttggatgctg gaatttgtgc tgataaaata gcacaaaaag aaaagataaa aaagctaaat
    10621 agaattattt ttttcatatc tatgaatatt tatacaaaaa atcttgttac ctgttttgat
    10681 tataaaacaa caggtattca ctgtttactg tttgtgtgtt gagcttaaat taggttgtac
    10741 ggtatgtatt tgatctagcc gtttcattga cacattgata ttgtaattat gtgtttggga
    10801 tatctaggtc aatatcatta taaaaaaaga ggaggaggtc ttcggtgcct atgttctaac
    10861 ttatgagaat gtatgtgtat tgagtaattt ctgtaaagta gtagaaacgt aagtatacta
    10921 aaacatacga gagaactaat ggagagcaca attatttgcc ctatttggtt gtgaatattc
    10981 catatccaat cagcttgagt atgcgagtgg gacttggaag agaatggatg cgagtgtgta
    11041 ataatggtat tattaacaat atgcgtatgc gaaagcaagg tagaacttac ccagtaagca
    11101 ataaatagga atgctaggaa tccgtgtata taggttgatt gcttgcgttt catattttta
    11161 tggtgcaaaa atacaacgga agaatgggag tattgtacca taaaaaaact atatgtagtg
    11221 atatttatct atttttatca ctacttgtag tgattgtagg atgaatgaga gagcattcat
    11281 accctttttc tttccaaaat gctaatgatt ttggtagtgc atagcgtaag tttttttcag
    11341 ccttcaatga gtcgtgaaat acaattatag aaccgtttct agaatatttt tttacatttt
    11401 gcaatattat ttctggaggc aacgtagcat cgtaatcgcg tgtaataaca tcccaaagaa
    11461 caattttaaa ggtgtgcttg agttttttta tctgtcgagg ggttatgtgt ccatgtggtg
    11521 gtctaaataa atcactattg ataagttttt gagcacgtgc aatatcttca aagaatattt
    11581 ttgtgtcggt aaacattcct cgtttgtggg tgtatgcatg attgcctact tgatgccctc
    11641 gttgttgaat ttctagaaac aagtcgggat atttttcaac attattgcca atacaaaaaa
    11701 atgtagcttt tacattatac gtatcaagca aatctaaaat ccaaggtgtt atttccggag
    11761 taggtccatc atcaaatgta agaaaaatct ttttttcttt ggcgtttcct ctccatatta
    11821 ctggttggaa gaaacgcctt atacattgag gaacttgttc tattagcata atgactatta
    11881 cctcaataaa tgatcaaata tacttgcata ttgcattaag cttgcatagt atttttcaaa
    11941 cgttttttcg tcgtaatttt gtgtgatata agcaatattt ctgagcgaag atagttgaat
    12001 atgagcatca tatgctgcag aagctttttt ctgtggactc aatttactta tccactctaa
    12061 ataggcaatg gatcgattac caatagtatt aattaaatca gttgctttct ctttttctcc
    12121 aagcatataa taatattcag ctaatgaggt agaagaaaag tcgtgaggca ctgtagtacc
    12181 tggaactact ttcatacaat agtccaatgc ttttaaagcc ctaatagaat ctccttcttc
    12241 tagtaaagct cctactaatg aagaaaacat ttgacgttgt acttttgaca tacgcagaat
    12301 attttcgtct aaatatacag aagggtcggc aatattgcca aatttaaact tattcatcat
    12361 attatcatac atcatgtctg tatgaactgt acctgttggt atagagtcgg tacgaggtac
    12421 tatacgataa gccaaacctt ctaactggaa atatttacgc agaccaagat gcatttctga
    12481 cccaactgtt acagcataat aaattggtcg ttcccaattg ttttgtgcta gcatttctag
    12541 aatcattaat tcactttttg ttagtaattg tctatctcgc aatttaaatt caatggcagg
    12601 caatacgcta cctttctcgt ctgcactaat ggctccttgt ttcactaaat tatctgcatt
    12661 tacagctaat gatagagttg tagcaggtat aaagtcgcgt gtgtcccctg ttcttgggtc
    12721 ttgcttggtg cgagggtcgt cgctagctaa gaaatgtaga gcgtctttta agtcgacagt
    12781 tccagtcatc agattatacg catatgcata gttgcgtttt ccttctccat atgtttctct
    12841 tccccaagaa ataggtaatg gagctgatgc atacgcagga cgtttcattt ggtcaatata
    12901 ccaatctgtg tttaaatagc ttaaattaca tacccgttta tcaagagaaa cggctgttcc
    12961 ttctacttct tgagtgtacc ataaagggaa tgtgtcgtta tctccattcg taaaaatgat
    13021 agcattggga gctagcgaat taaaatagtt ttgagcaaaa tcgcgtacta catatctatt
    13081 tgaacggtca tggtcatccc aattttgttg agccataata ataggagcag gagcagctaa
    13141 taaaagggct ataatggctg ctaatgtagg gtttatcttt tttgattcaa gccatttggc
    13201 tataccagca acaccaaaac ctatccaaat tgagaatgca taaaacgatc cagcataggc
    13261 atagtcgcgt tctcttggtt ctgaaggtcc ttgattgagg tacatgacaa tggcaatccc
    13321 tgtcataaag aataaccaac caataaccca aaaggcttga ttcccttttt tgtttgacca
    13381 cattagttga tatattatcc caattattcc tagtagtaaa ggaagcatga aataggcgtt
    13441 atgagcttta ttttctgcca aatctggtgg tagatttgtt tggtctccca ccagatagtt
    13501 atcaataaaa ttaaatccag ttatccaatt gcctcgagta acctctccac tgccttgtaa
    13561 atcattttga cgtccagaaa aattccataa gaaataacgc caatacatat aattgagttg
    13621 gtaagagaaa aagaaacgaa ggttttcacc aaatgtaggc actactacat caatgattct
    13681 gccacattta tcatatttta cggttttccc ctgtatagta atccaactat tatatgcttt
    13741 tatatgactt tcattagagc tgtgtagacg aggaaaaatt gttttaaatt gactatcgta
    13801 ttccaattca aatttggttc ctgtaacgat atacctgtcc ttttcattag gggatgtttt
    13861 ttctgcttga ccccatgttt ttgcaccttc ttttacagat ggtttacagt agccatcttc
    13921 ttcgattaac ttaggttcgg cagagtaata tgctccgtaa aataatggtc gatcaccata
    13981 ttgttcgcga tttagatagc tttgtaacgc aaaaatattg tcgggtgagt tttggtccat
    14041 gggaggattg gcggctgaac gaataacaat tagtgcataa cttgaatagc caattagcat
    14101 cattgttaac gaaataatga tggtgctcaa taggcgaata ctaatttttt ctttcaattt
    14161 tataaaataa aaaatgagta tactgagtaa tacaagccct agtaacaaac tgcctaatag
    14221 aaaagggata cctaataaaa tgagagatcc gatgaatgaa actttaatgc gagtggcaga
    14281 tggttttttg ctatataatt caatgatagc ccacactaaa acggcaattg tagtaaaata
    14341 atagaaaaaa gctcctgtat taaagctaaa acctaaggta ttgacaaaga ataattcggc
    14401 aatgcctgcg tttgctataa attgagggat aaaaccatac atgatgaacc caagtatagc
    14461 aaatgaaata agtagtgccc atgttccacc tttcaacgat actttttctg tttttctgaa
    14521 ataatatacc aaaactaaag ctggtatagt taataagtta agtaagtgaa caccaataga
    14581 ta
//
LOCUS       DAOSRP010000039        14564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4217_length_14564_cov_5.248604, whole genome shotgun sequence.
ACCESSION   DAOSRP010000039 DAOSRP010000000
VERSION     DAOSRP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4217_length_14564_cov_5.248604"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="PLJ82_03715"
     CDS             complement(<1..1549)
                     /locus_tag="PLJ82_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOR40645.1"
                     /translation="MKPFLQQVASVFYHTYKEDLTDFCFVFPNRRSGLFFEKYLMETA
                     QHTFFSPTITSISDFCASHSMLQLEDKMGLLFRLYGIYQSIANKHETFDSFASFGEIL
                     LSDFDDIDKYLVNAEDIFQNVKELQSINYLPDYLTNEQKIIIQRFWNNFNPEPKRQQE
                     FLATWEILFPLYQSFKDDLKKNNLAYEGMLLRELAENGIHASYNKIVFVGFNALSKVE
                     KTLFLQLKKQKKADFYWDYEGEFVQDKTNKAYTYVAENLMLFPSQLKIEKEIIGNKYV
                     EAIAIPSSVGQAKQVHQILQHLGIENISTETAIVLSNENLLLPMLFSLPNHIDKVNIT
                     MGYPLVFTPIKELIDNLLALQKSANTNTEGKTFFYYKQVLSLLSHRYIYNENNTNYQE
                     LIKKIHSNNWIRIPALNLQIDTLLCLLFKPCNTASLCDYLIAIIGHLLAERKSVSINN
                     SDENNQLMEQEFLYQCFIALQRMQDLLNQWNIPLSLDTLSKLIKRLLSTIVISFEGEP
                     LAGLQIMG"
     gene            1657..3603
                     /locus_tag="PLJ82_03720"
     CDS             1657..3603
                     /locus_tag="PLJ82_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR40646.1"
                     /translation="MISIEGLTVEFGAKALFDNISYVVNKKDRIALVGKNGAGKSTML
                     KIIAGLQEPTRGQCIIPKDLTIGYLPQHMIHNDDATVYEEAEKAFAHILEIQEQIEKN
                     NILLCERDDYESDSYHSLIEEITLLNEQLAMLGGVNYVADIERTLFGLGFLRTDLARP
                     TKEFSGGWRMRIELAKLLLQRPDVLLLDEPTNHLDIESIQWLENFLASRANAVMLVSH
                     DKAFINAVTTRTIEISLGKIYDYRANYSTYLELRKERREQQLRAYENQQKQIQDTEEF
                     IERFRYKTSKAVQVQSRIKQLDKIDRLEVDEEDTSSLRLKFPPAPRSGSFPVIAEGLA
                     KLYGNYVVFSNVDLTIKRGEKVAFVGKNGEGKSTLVKCIMNEIEYAGKLTLGHNVQIG
                     YFAQNQASLLNESKSVFETIDDVAVGDIRTKIRDILGAFMFGGEEADKKVKVLSGGER
                     SRLAMIRLLLHPVNLLILDEPTNHLDIRSKDVLKQAIKEFDGTVIVVSHDREFLDGLV
                     TKVYEFADKKIIEHIGGVYEFLHKKKLNTVQEIERKNLPNKDIPTSTQQDTEQKLSYE
                     ERKERIKILRKAEKILETCEQDIAKLEKELETIEQAMLQPENAANVSFVASYTQKKRA
                     LEQKYYEWELLAEDVETKKNELSN"
     gene            3627..3935
                     /locus_tag="PLJ82_03725"
     CDS             3627..3935
                     /locus_tag="PLJ82_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOR40647.1"
                     /translation="MQFNNVSIEKKANVYFNGNVTSRTVLFPDGTRKTLGIMMPGEYE
                     FGTDSAELMEMLGGNMDVLLPNESEWKSYAEGDSFHVPAKSSFKLKVNTVVDYCCSYL
                     "
     gene            complement(3944..>4152)
                     /locus_tag="PLJ82_03730"
     CDS             complement(3944..>4152)
                     /locus_tag="PLJ82_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40648.1"
                     /translation="MLASVAKMEQDEQNGMKPDNVAKTIVKQVMAKKMGKVIVPGISN
                     KILYGLAHILPTKLLLALLHKLYN"
     assembly_gap    4153..4162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4163..4752)
                     /locus_tag="PLJ82_03735"
     CDS             complement(<4163..4752)
                     /locus_tag="PLJ82_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR40649.1"
                     /translation="MVQNKIVLITGASSGIGLVTAVQLAEQGYIVFASSRRGNSSVNL
                     PNLHFVQADVNDKDQMKNVIELIVKQYKKIDVVICNAGNGIAGAIEDCSSDEIRFQME
                     TNFYGVVNTIQQCLPYLREQKNGKILVISSLAAIFPIPYQAFYAASKAAVHILTQALS
                     IEVKRFNIQVCSILPGDTKTNFTKARSYAVQAQSPTS"
     gene            complement(4812..7352)
                     /locus_tag="PLJ82_03740"
     CDS             complement(4812..7352)
                     /locus_tag="PLJ82_03740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOR40650.1"
                     /translation="MEQKTYTFDEALQASIPYFEGDELAAKVWINKYALKDSYGNIYE
                     KTPDDMHWRLANEIARIESKYPNALTADELFELMRNFKYIVPQGSPMTGIGNHHQIAS
                     LSNCFVIGFTGESDSYGAVIKIDEEQVQLMKRRGGVGHDLSHIRPNGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLSVSIKHPDAESFIDAKLEQGKVTGANVSVKI
                     HDDFMNAIINKVPYVQQYPVFSKEPKFKKEIDAEQLWKKIVHNAWKSAEPGVLFWDTI
                     IKESLPDCYADLGYQTVSTNPCGEIPLCSYDSCRLLALNLYSYVENPFTSEAKFNFEL
                     FKKHSQLAQKIMDDIIDLEMEKIDKILEKIASDPEDASIKLTEEQLWLKIKNKTEKGR
                     RTGVGTTAEGDMLAALGLRYGSEKATEFSELVHKTLAIEAYRSSVTMAKERGAFEVYD
                     TEREKNNPFVLRLKDADPELYSEMAKYGRRNIACLTIAPTGTTSMMTQTTSGIEPVFM
                     PVYKRRRKVNPNDQDTRVDFVDELGDTWEEYMIFHHKFVTWMEANGYPTAKKYTSEEI
                     EELVKKSPYFKATSADIDWMQKVHMQGRVQKWVDHSISVTINLPNDATEELVGNLYIE
                     AWKSGCKGCTVYREGSRSGVLISANSEKKEKDPCNCYPEITPKRPKELECEIVRFQNN
                     KEKWIAFVGLLDGRPYEIFTGLADDDDGILLPKNITSGKIIRNYNEETGVKSYDFQFS
                     NKRGYKTTIEGLSHKFNPEYWNYAKLISGVLRYGMPIDQVVRLVNGLQLNNESINTWK
                     NGVERALKKYIPDGTQAKGQVCPKCGDTLVFEEGCLICKKCGYSKCGS"
     gene            7674..8417
                     /locus_tag="PLJ82_03745"
     CDS             7674..8417
                     /locus_tag="PLJ82_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR40651.1"
                     /translation="MISLLKNRATVRQYSDKNISEELLIDLLEVSFRSSNTGNMQVYS
                     VVVTRDEEMKKQLAPCHFNQPMIVQAPIVLTFCADFNRFTKWCEYNKAIPGYDNFLSF
                     ITATIDATIVAQTFAIAAESKGLGICYLGTTNYFAEKISSILHLPRFVVPITTITVGY
                     PASIPNQTDRLPTRSLVHYEQYTDYTEDAIRNMYAEKENLPEMKQFVLDNKKETLAQV
                     FTDVRYTKSNNETFSAMLLSFLQKQGFRV"
     gene            complement(8487..8906)
                     /locus_tag="PLJ82_03750"
     CDS             complement(8487..8906)
                     /locus_tag="PLJ82_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOR40652.1"
                     /translation="MLKNILSVTGKPGLYKLLSGNKKMIIAESLIDGKKSPVHSHEKV
                     ISLADIAIYTDTKEVPLREVFATLLEKSEGKESLDSKVSSTDELKKHFATVLPDYDRD
                     RVHNSDIKKIYAWYNLLIKNNITDFSEEKENEEVVSE"
     gene            complement(8911..9498)
                     /locus_tag="PLJ82_03755"
     CDS             complement(8911..9498)
                     /locus_tag="PLJ82_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOR40653.1"
                     /translation="MKIDIILSGVGGQGILSIAAVIGQAALENKHYMKQPEVHGMSQR
                     GGDVQANLRISDKPIFSDLIPLQQADLILSLEPMEALRYMPYVKETGWIVTNETPFKN
                     ISNYPNEGDIEDEIKKHKNHILINIDEIAKKVGSARTANMVLLGAALPFLSLPEEDIE
                     DAISAIFEKKGEEIIKLNIKALRAGAEISRNILNK"
     gene            complement(9502..11082)
                     /locus_tag="PLJ82_03760"
     CDS             complement(9502..11082)
                     /locus_tag="PLJ82_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOR40654.1"
                     /translation="MQNLLLLLGDEAIAQGAIDGGISGVYAYPGTPSTEITTYIQETT
                     DKREIRSDWSVNEKTAMEAAMGLSYVGKRALVCMKHVGLNVAADAFINAAITGINGGI
                     VVLVADDPSMHSSQNEQDSRFYGKFALIPILEPSNQQEAYDMAYTGFELSEKLGYPIL
                     MRITTRLAHSRSGIIRKPQKQQRPISLPTNRKQFILLPAIARQRYSALLAAQSEFEEQ
                     ANNSLYNEYIDGVNKKMGIIACGLTFNYLMENFPNGCEYPIVKIGQYPLPRKKIERLI
                     DECESILVIEDGYPLVEELLKGYTGKGIQVKGKLDGTLPRQGELNPNNVGQAFGFTLE
                     KDITIPEVVKMRPPSLCKGCGHIDLYESLVEVLKDYPTARVFSDIGCYTLGALPPYTA
                     IDTCLDMGASITMAKGAADGGLHPSVAVIGDSTFTHSGITGLIDCVNDNSNVTIIIVD
                     NETTAMTGGQTSSGTGKLEAICQAVGVDENHIHVCEPLKKNIDYIVQLIKKEISHTGV
                     SVIIPRRECIQTLKRKKK"
     gene            complement(11098..12603)
                     /gene="dnaB"
                     /locus_tag="PLJ82_03765"
     CDS             complement(11098..12603)
                     /gene="dnaB"
                     /locus_tag="PLJ82_03765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR40655.1"
                     /translation="MEKTKKPRKKEPEYGKVPQYVVSEYGGKLPPQAIELEEVVLGAL
                     MLEKDAYVAVCEFLKKECFYKDVHQTIYEAITNLSLNQEPVDLLTVSQQLKRMGKLEE
                     VGGQSFISELTGKVASAANIEYHAQIIVQKYLSRELIRISSDIQKKAYDEKQDVDELM
                     QDAEGQLFEITQRNLKKDVTHIQPVISKAIDLIEIAANKQDGYTGIPSGFNGIDTITS
                     GWQKSDLIIIAARPAMGKTAFVLSMAKNMALDYEHPVAVFSLEMSNIQLVNRLIVNVA
                     EIEGDKIKRGKLAPFEWEQLRLRTATLQKAPIFVDDTPSLSVFELRTKARRLVKEHKI
                     QCIIIDYLQLMNASGMTYGSREQEVSIISRSLKGLAKELDIPIIALSQLNRGVEGRTG
                     ADKRPQLSDLRESGAIEQDADMVLFIHRPEYYKITQDEQGNDLRGKAEIIIAKHRNGS
                     TGDVFLRFKQDFIKFTNLASEESYAEFQSKSTDSQEHDFLQANTYSNPSPF"
     gene            12723..13538
                     /gene="ispE"
                     /locus_tag="PLJ82_03770"
     CDS             12723..13538
                     /gene="ispE"
                     /locus_tag="PLJ82_03770"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOR40656.1"
                     /translation="MITYPNAKINIGLFVTEKRTDGYHNLETIFYPIPLKDKIECHIS
                     DTNDSSLIISGIRIDGNTSDNLVMKAYNLLKLSHNLPNLNFTLEKNIPFGAGLGGGSA
                     DAAFVLTLLNTTYNLGLSIAELEYIASKIGADCPFFIQNKPVFATGIGNEFHPISFSL
                     SGYFLVLVKPPIHVSTPQAYKNINPQKPDFDLKTIHLLPIKEWKHVIFNDFETNVFIQ
                     HPEIEQIKDSLYHQGALYASMSGSGSSVFGIFPKQVNITNLFQNSFVWSGFLA"
     gene            complement(14369..>14564)
                     /locus_tag="PLJ82_03775"
     CDS             complement(14369..>14564)
                     /locus_tag="PLJ82_03775"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOR40657.1"
                     /translation="YSYKELAEKIGQPSSARAIGNACRKNPFVIVIPCHRVICHNGKL
                     GGYIGGIEVKKQLVYNEKKG"
BASE COUNT         4576 a         2461 c         2454 g         5063 t
ORIGIN      
        1 ttcccataat ttgtaatcca gccaacggtt ctccttcaaa cgagataaca atagtactaa
       61 gtaatcgttt aataagcttg cttagtgtat ctaatgataa tggaatgttc cattggttaa
      121 gtaaatcttg catacgttga agagcaataa aacattggta tagaaactct tgttccatca
      181 attgattgtt ttcatcagag ttgtttatgg aaactgattt tctttctgcc agtaaatgcc
      241 caataatagc tataagataa tcacataatg aggctgtgtt acaaggctta aatagtaagc
      301 acaataacgt gtctatttgt aaattgagag ctggtatacg tatccagtta ttactatgta
      361 tcttctttat aagctcttga tagttagtat tgttttcgtt gtaaatatat ctatgtgata
      421 ataaagataa aacttgttta taatagaaaa aagtttttcc ttcggtgttt gtattggctg
      481 atttttgtaa ggctaataaa ttatcaatta attctttaat aggagtaaat actaggggat
      541 agcccatggt aatatttacc ttatctatgt gattaggtaa tgaaaaaagc attggaagaa
      601 gaagattttc attagaaagc actatggctg tttctgtact tatattctca ataccaagat
      661 gttgcaaaat ttgatgaact tgttttgcct gaccaacact tgatggaatg gcgattgctt
      721 caacgtattt atttccaata atttcttttt caatttttaa ttgagacgga aatagcatta
      781 aattttcagc tacataggta taagccttat ttgttttatc ttgcacaaat tctccctcgt
      841 agtcccaata aaaatcagcc tttttttgtt tttttagttg taagaataat gttttttcca
      901 ctttactcaa agcattgaat ccaacaaata ctattttgtt ataggaagca tgtatgccat
      961 tttcagccaa ttcgcgtagc aacatacctt catatgccaa attattcttt tttaaatcat
     1021 ctttaaagga ttgatataaa ggaaacaata tttcccatgt agccaaaaat tcttgttgtc
     1081 gtttaggttc aggattaaag ttattccaaa agcgttgtat aattattttt tgttcgtttg
     1141 ttaaataatc gggcaaatag ttaatggatt gtagttcttt tacattttga aatatatctt
     1201 cagcatttac taaatattta tcaatgtcat caaaatcact taacaaaatt tctccaaagg
     1261 atgcaaatga atcaaacgtt tcatgtttat ttgcaatgga ttgatatata ccatataatc
     1321 gaaacaataa acccatttta tcttcaagtt gcaacataga gtgcgaagca caaaaatcgg
     1381 aaatgctagt aattgtaggc gaaaaaaagg tgtgttgggc tgtttccatt aggtattttt
     1441 caaaaaatag tccagaacgc ctatttggaa acacaaagca aaaatcagtc agatcctctt
     1501 tatatgtgtg gtaaaataca gaagcaactt gttgtaaaaa tggtttcata agtagtatac
     1561 taaggatagc aacaaaaata catataatat tgcgaaaaaa acgattacaa tgagattaaa
     1621 atttctgtat tgaaaaatac catttatctt tgcattatga tttcaataga aggacttaca
     1681 gtagaatttg gtgcaaaagc attgtttgat aacatatcat atgttgtaaa caaaaaagac
     1741 agaatagcat tggttggtaa aaatggtgct ggaaaaagta caatgcttaa aattattgca
     1801 ggcttacaag agcctacacg tggtcagtgc attattccta aggatttaac catcggttat
     1861 ttaccacagc atatgattca caacgatgat gccaccgtat atgaagaagc tgaaaaggct
     1921 tttgcccata ttttggaaat tcaagagcaa atagagaaaa ataatatatt actatgcgaa
     1981 cgagatgatt atgaatctga ttcataccat tcgcttattg aagagattac acttctcaac
     2041 gaacaacttg ctatgttggg tggggtgaat tatgtggctg atattgaacg aaccttgttt
     2101 ggtttaggat ttttacgcac agatttggca cgacctacca aggaatttag tggtggttgg
     2161 cgtatgcgaa ttgaattagc aaaattgttg ctccaacgcc ctgatgtatt actgttagat
     2221 gaaccaacga atcatttgga tatagaatcg atacaatggt tagaaaattt tttggcaagt
     2281 agagccaatg cggttatgtt agtttcgcat gataaagcct ttattaatgc tgtaactaca
     2341 cgcacaattg aaattagttt gggtaaaata tacgattata gagctaatta ttcaacgtac
     2401 cttgaacttc gcaaagaacg tagagaacaa caattgcgtg cgtatgaaaa tcaacagaag
     2461 caaatacaag ataccgaaga atttattgag cgttttcggt acaaaacttc taaagctgtt
     2521 caagtacaaa gtagaattaa acagctagat aaaattgaca gattagaggt agatgaagag
     2581 gatacgagtt cattacgtct caaatttcca cctgctccgc gttctggaag ttttcctgta
     2641 attgctgaag gtcttgccaa attatatggc aattatgttg tgttctcaaa tgtcgacctt
     2701 actattaaac gaggagaaaa agtggcattt gtaggaaaaa atggagaggg aaagtcaact
     2761 ttggtgaaat gtatcatgaa cgagattgag tatgctggta agttgactct tgggcacaat
     2821 gtgcaaatag gttattttgc tcaaaatcaa gcttctctgt taaacgaatc gaaatctgtt
     2881 tttgaaacta ttgatgatgt ggctgttggg gatattcgta caaaaattcg ggatatatta
     2941 ggtgcattta tgtttggtgg cgaagaggct gataaaaaag taaaagtgct ctcaggagga
     3001 gaacgcagtc gcttggcaat gattagatta ttgttgcatc cagttaattt gcttatttta
     3061 gatgagccaa caaaccattt agatatacgt tcaaaagatg tattaaaaca agctatcaaa
     3121 gagtttgatg gaactgtgat tgtggtttct cacgatagag aatttttaga tggtttggta
     3181 acgaaggtgt atgaatttgc tgataaaaaa attattgaac atataggcgg tgtgtatgag
     3241 tttttacata aaaagaaact taataccgtg caggaaattg agagaaaaaa tttgcccaac
     3301 aaagatatcc ccactagtac acaacaggat acagaacaaa aattatctta tgaggaacga
     3361 aaagagcgta taaaaatact aaggaaagcg gaaaaaatac tggaaacttg cgaacaggat
     3421 attgcaaaac tcgaaaaaga gctggaaaca atagaacaag ccatgttgca acctgaaaat
     3481 gcggctaatg tttcttttgt tgcatcctat acgcagaaaa aacgagcttt ggagcaaaaa
     3541 tattatgagt gggaattatt ggcagaagat gtggaaacaa aaaaaaatga attatcaaat
     3601 taatataact atcaaatccg tttattatgc aatttaacaa cgtaagtatt gaaaaaaaag
     3661 ccaacgtata ttttaatggc aatgttacta gccgaacagt attatttcct gatggtacac
     3721 gtaaaaccct aggtataatg atgccaggag agtatgagtt tggaacagat agtgctgaat
     3781 taatggaaat gttgggaggt aatatggatg tattactacc taacgaatca gaatggaaat
     3841 cttatgctga aggagattcg tttcacgtac ctgctaaatc gtcgttcaag ttaaaagtga
     3901 atacggttgt agattattgt tgctcttatt tgtaggttac atgttagtta tacaatttat
     3961 gcagtagtgc cagcaatagt ttggttggta atatatgagc taatccgtac aagattttat
     4021 tcgaaatacc tggtacgatt actttcccca tttttttagc cattacttgt tttacaatag
     4081 tttttgctac attatcgggt ttcattccgt tttgttcatc ttgttccatt ttggcaacag
     4141 aggcaagcat ttnnnnnnnn nngaagtagg ggattgggct tgtacggcat acgaacgtgc
     4201 tttggtgaag tttgttttcg tatctccagg caagatgcta catacttgaa tgttaaaacg
     4261 ctttacttca atcgaaagag cttgtgttag tatgtgtaca gctgctttgc ttgcagcgta
     4321 gaatgcctga tagggtatgg ggaatattgc agccaacgaa gagataacta gtattttccc
     4381 attcttttgt tcacgtaaat aaggtaaaca ctgttgaatt gtatttacta caccatagaa
     4441 atttgtttcc atttgaaaac gaatttcatc actactacag tcctcgattg cccctgcaat
     4501 tccattgcca gcattacaaa taacaacatc aatttttttg tattgcttta cgattagttc
     4561 aataacattt ttcatttgat ccttgtcgtt gacatcggct tgcacaaaat gcaggttagg
     4621 aagattaaca gatgagttgc ctcttctaga gcttgcaaag actatatacc cttgttcagc
     4681 aagttgaaca gcggtaacta gtcctatacc cgaactagca cctgtaatta acactatttt
     4741 attctgtacc atacaagaaa ttaacatgtt agggcggtta ttacacaccc taacagagta
     4801 gattaagtag attagctacc acattttgag taaccacatt ttttacaaat caaacaccct
     4861 tcttcaaaaa ctaaagtgtc accgcatttt gggcatactt gtccttttgc ttgggttcca
     4921 tcaggaatgt atttttttaa tgctctttca acaccatttt tccaagtgtt aattgattcg
     4981 ttgttaagtt gcagaccatt aactaatcta acaacctgat ctataggcat accataacgt
     5041 aatactccag aaattagttt ggcataattc caatattctg ggttaaattt atgtgataaa
     5101 ccttcgatag ttgttttata tccacgtttg ttagagaatt ggaaatcata actcttaacc
     5161 ccagtttctt cattgtaatt acgaataatt tttccactcg taatgttttt aggtaataaa
     5221 atgccatcat catcgtcagc tagccccgtg aaaatttcgt acggtcttcc atctaataat
     5281 ccaacaaaag caatccattt ttctttgttg ttttggaaac gaacaatctc gcattccaat
     5341 tctttagggc gtttgggtgt tatttctgga taacaattgc atggatcttt ttcttttttc
     5401 tcactgttag cagagataag aacaccagag cgtgagcctt ctctgtaaac cgtacatccc
     5461 ttgcaaccac ttttccatgc ttcgatataa agatttccta ccaattcttc tgttgcatcg
     5521 ttcggtaaat taatggtaac actaatagaa tggtctaccc atttttgaac tctaccttgc
     5581 atatgtactt tttgcatcca gtcaatatcg gctgatgtag ctttgaaata aggtgatttt
     5641 tttactaatt cttcaatttc ttcggatgta tactttttgg cagtaggata tccatttgct
     5701 tccatccatg ttacaaattt gtggtggaaa atcatgtatt cttcccaagt atcaccaagt
     5761 tcatctacaa aatcaacgcg tgtatcttga tcgtttggat ttacttttct acgacgttta
     5821 tacaccggca taaatactgg ctctatacca gatgttgttt gagtcatcat acttgtggtt
     5881 ccagtaggag caatggttaa acatgcgatg tttctgcgtc catattttgc catttcactg
     5941 tataattcag gatcagcatc ttttaaccgc aatacaaatg gattgttttt ttctctttct
     6001 gtatcatata cctcaaaagc ccctctttcc ttagccattg ttacagacga acgatatgct
     6061 tctatagcta atgttttatg tactaattcg gaaaattctg ttgctttttc acttccgtag
     6121 cgtaatccta aagctgccaa catatctcct tctgcagttg tcccaacacc cgttctacgt
     6181 cctttttctg ttttgttttt aatttttagc cacagttgtt cttctgttaa tttaatgcta
     6241 gcatcttctg ggtcagaagc aattttttct agaattttat caattttttc catttccaaa
     6301 tcaataatgt catccattat tttttgtgct aattgagaat gttttttgaa taattcaaaa
     6361 ttgaatttgg cttcagaagt aaatggattt tccacataag aatataagtt caatgccagt
     6421 aaacgacaac tatcatacga acataaagga atttcaccgc aagggttagt agataccgtt
     6481 tgataaccta aatcggcata acaatctggt aaagattctt ttatgattgt atcccaaaat
     6541 aaaacacctg gttcggcaga tttccatgcg ttgtgaacaa tctttttcca taattgttca
     6601 gcatctattt cttttttaaa tttgggttct ttcgagaata ctggatattg ttgaacatat
     6661 ggaactttat tgatgatagc attcataaag tcatcatgta tttttactga tacattcgct
     6721 cctgttactt ttccttgctc tagctttgca tcaataaatg actctgcatc ggggtgttta
     6781 attgatacac ttaacattaa tgctcctctt cttccgtctt gggctacttc gcgtgtagag
     6841 tttgaatagc gttccataaa cggaacaata cctgttgatg taagtgctga attttttact
     6901 ggagaaccat ttgggcgaat atgagacaaa tcatgcccaa cgccacctct acgtttcatc
     6961 aattgcactt gttcttcatc tatttttatt acagctccat acgaatcgga ctcgccagta
     7021 aaaccaataa caaaacaatt ggataaggat gctatttggt gatgattgcc aatacccgtc
     7081 atggggcttc cttgtggaac aatgtacttg aaatttcgca ttaattcaaa caattcatca
     7141 gctgttaatg catttggata ttttgattca atacgtgcta tttcatttgc caaacgccaa
     7201 tgcatatcat caggtgtttt ttcgtaaata ttcccgtaag aatcttttag tgcgtatttg
     7261 tttatccata cttttgctgc taactcatct ccttcaaaat aagggattga tgcttgcaat
     7321 gcctcatcaa atgtgtacgt tttttgttcc atttttttct aaaatattga ttgtatgtcg
     7381 attataaaaa gttggtttcg tgcttcacgg gactacaatg ttacactatt ttttttactt
     7441 aacaaagttt tttataaata aaaatgtaaa ataattgcag taaatttatt tttctttttt
     7501 gtaaagttgt caaaaatatt ttataaatac atgattatta gtattttatt aatattttta
     7561 gatgaaaaag ttgtcaaaaa aaaatcacat taatttaata ttcgtaattt atttaatttg
     7621 tttattgaga gaaattgagt atttttgtaa attaataaat acatatatac catatgatta
     7681 gtttacttaa aaatagagct actgttcgtc agtattcgga taaaaacata agtgaggaat
     7741 tgttaataga tttattagaa gtttcattta gaagttcaaa tacgggcaat atgcaagtat
     7801 atagtgtggt tgtaacacgc gatgaagaaa tgaaaaaaca attagctcca tgtcatttta
     7861 atcaacccat gattgtacaa gctccgatag ttttaacttt ttgtgccgat tttaaccggt
     7921 ttactaaatg gtgtgaatac aacaaagcca ttccagggta tgataatttc ttgtcattta
     7981 ttacagccac aattgatgct accattgttg ctcaaacctt tgctattgct gcagagtcaa
     8041 aaggcttggg catatgttac ttaggtacta ccaattattt tgccgaaaaa attagcagca
     8101 tattacattt acctcgtttt gttgtgccta ttacaactat tacagtaggt tatccagctt
     8161 ctataccaaa ccaaacagat agattaccaa cgagatcatt ggtgcattac gaacagtata
     8221 ctgattatac agaagatgct attagaaaca tgtatgcaga aaaagaaaac ctgcctgaaa
     8281 tgaaacaatt tgttttagat aacaaaaaag aaacactggc tcaagtattt acggatgttc
     8341 gttatacaaa aagtaataac gaaacatttt ctgcaatgtt attgagtttc cttcaaaaac
     8401 aaggttttag ggtataacac aatctgtttt aattttcatc gtttacgtct ttgctcctta
     8461 gctataacct aaacgatgaa acatatttat tcagatacaa cttcttcgtt ttccttttct
     8521 tccgagaaat ctgtaatatt atttttgatt agtaggttat accaggcata tatttttttg
     8581 atatcactat tatgcactct atctctatca taatcaggta aaactgttgc aaagtgtttt
     8641 tttagttcat cggtgctaga tacctttgaa tcaaggcttt ctttcccttc acttttttca
     8701 agcaacgtag caaacacttc acgcagggga acttctttag tatcagtata aatggcaata
     8761 tctgccaacg aaattacttt ttcgtgcgag tgcacaggcg attttttacc atcaattaat
     8821 gattcggcta taatcatttt tttatttcca gacaataatt tatataaacc aggttttcca
     8881 gtaacagata aaatattttt cagcatatta ttatttattt aaaatgtttc tacttatttc
     8941 agctccagca cgtaaggctt tgatgtttaa tttaataatt tcttctcctt ttttctcaaa
     9001 aatggcagat atagcatctt ctatatcttc ttctggtaaa gataaaaatg ggagagcagc
     9061 tcctaataaa accatatttg cagtgcgtgc agaacctact ttttttgcta tttcgtcaat
     9121 attaattaaa atatgattct tgtgtttctt tatttcatct tcaatatccc cttcgtttgg
     9181 ataattgcta atatttttga atggagtttc attagttact atccatccag tttcttttac
     9241 ataaggcata taacgcaatg cttccatcgg ttcgagagac aaaataaggt cagcttgttg
     9301 caaaggtatt aagtcagaga aaattggctt atccgaaata cgtaaattgg cttgtacatc
     9361 tccaccacgc tggctcatac catgtacttc gggttgtttc atataatgct tgttttcaag
     9421 agcagcttgt ccaataacgg cagcaataga taaaattcct tgaccaccta cacctgataa
     9481 aatgatgtct attttcatag tttatttctt tttacgtttt aaagtttgta tacactctct
     9541 gcgaggaata attacggaca ctccagtatg gctgatttct tttttgataa gttgtactat
     9601 gtagtcaata tttttcttta gtggttcgca tacatgtata tgattttcgt caactcctac
     9661 ggcttgacaa attgcttcaa gttttcctgt cccagacgat gtttgtccac cagtcattgc
     9721 cgttgtttcg ttatcaacaa taatgatggt aacattgcta ttgtcattta cacaatctat
     9781 tagtccagta atacctgagt gggtaaatgt agaatcacca attactgcaa cagatggatg
     9841 caaaccacca tcggctgccc cttttgccat cgtaatagat gctcccatat ccaaacaagt
     9901 atcaatggca gtatagggag gcaatgctcc taaagtataa caaccaatgt cgctaaaaac
     9961 acgagcagta ggatagtctt tgagcacttc taccaatgat tcgtacaaat caatatgccc
    10021 acatcctttg caaagcgatg gtggacgcat ttttactact tctggtatag taatgtcttt
    10081 ttctagagta aacccaaaag cttgccccac attattgggg ttgagttctc cttgtcgagg
    10141 tagcgtgcca tcaagttttc cttttacctg tatgcccttt cctgtatagc cttttagtaa
    10201 ttcttccact aggggatatc catcttcaat taccaatatg ctttcgcatt cgtctattaa
    10261 cctttcaatt tttttacgag gcaatgggta ttgtccaatt tttacaatgg gatattcaca
    10321 tccgttgggg aaattctcca tcaggtaatt aaatgttaac ccacaggcta ttattcccat
    10381 ttttttgttc actccatcaa tatattcatt gtatagtgag ttatttgctt gttcttcaaa
    10441 ttcactttgt gctgccaaca aagcactata acgttgtctt gcaatggcag gtagtaaaat
    10501 aaactgtttt ctatttgtgg gtaacgatat agggcgttgt tgcttttgtg gtttacgtat
    10561 aatgcctgat cgggaatgag ctaatcgtgt ggtaatacgc attaatattg gatagcctaa
    10621 tttttctgaa agttcaaaac cagtataagc catatcatat gcttcttgtt gattactagg
    10681 ttcaagaatt ggaataagtg caaatttacc ataaaaacgt gaatcttgtt cgttttgaga
    10741 cgaatgcatg gatgggtcat cagctactaa aactactatt cctccattta ttcctgtaat
    10801 ggcagcatta ataaaggcat ctgcagcaac attcaagcca acatgtttca tacatactaa
    10861 ggctcgtttt cctacataag ataaacccat agcagcctcc atagctgttt tttcatttac
    10921 cgaccaatcg ctacgtatct ctcttttgtc ggttgtttct tgaatgtagg ttgttatttc
    10981 ggtagatggg gtgcctggat aagcatacac acctgaaata ccaccatcaa tagctccttg
    11041 tgctattgct tcgtctccta ataataataa taaattttgc atgaatataa atatagttta
    11101 aaatggtgaa ggattagaat acgtatttgc ttgcaaaaaa tcatgttctt gactatctgt
    11161 acttttactt tgaaattcag catacgattc ttctgatgca agattggtaa atttaataaa
    11221 gtcttgtttg aaacgtaaaa atacatcgcc tgtagaacca tttcgatgct tggcaataat
    11281 aatttcggct ttacctctta aatcgtttcc ttgttcgtct tgtgtaatct tataatattc
    11341 agggcgatgg ataaacagta ccatatccgc atcttgttct attgctcccg attcgcgcag
    11401 gtctgataat tgagggcgtt tgtcagctcc agtacgtcct tctacaccac gatttaactg
    11461 tgataaagca atgattggaa tatcaagttc ttttgccaag ccttttaatg acctcgaaat
    11521 aatacttacc tcttgttcac ggcttccata cgtcatgccg ctggcattca ttaattgcaa
    11581 gtagtcgatt ataatacatt gtattttatg ctctttaact aatcggcggg ctttggttcg
    11641 taactcaaat actgaaaggc taggggtatc atcaacaaaa attggggctt tttgcaatgt
    11701 tgcagttcgc aaacgcaatt gttcccactc aaatggagct agctttccac gtttaatttt
    11761 gtctccctct atttcagcta catttacaat taaacggtta actaattgta tattagacat
    11821 ttcaagagag aaaacagcta ctggatgttc ataatctaaa gccatatttt ttgccataga
    11881 cagtacaaaa gccgtttttc ccattgcagg acgtgcagct ataataataa ggtcggattt
    11941 ttgccatcca gatgtaatcg tatcaatgcc attaaagcct gatggaatgc ctgtatatcc
    12001 atcttgtttg ttggcagcaa tttcaatcaa atcaatagct ttgcttatga ctggttgaat
    12061 gtgtgtaacg tcttttttaa ggtttcgttg tgtaatttca aaaagctgtc cttcagcatc
    12121 ttgcattaat tcatcaacgt cttgcttttc atcatacgcc ttcttttgga tatcagacga
    12181 gatacgtatt aattctctcg acaaatactt ttgaacaata atttgagcat gatattctat
    12241 attagcggca gaagccactt tccctgttag ttctgaaata aaagattgtc ctcctacttc
    12301 ttctaatttt cccatacgtt ttaattgttg ggatacagtt agtaggtcaa caggttcttg
    12361 atttaacgat agattggtaa ttgcttcgta tattgtttgg tgtacatcct tgtaaaaaca
    12421 ttcttttttg agaaattcgc acaccgctac ataagcatct ttttctaaca tcaaagcccc
    12481 taaaaccact tcttccaatt caattgcttg aggtggcagt tttcctccgt attcagatac
    12541 aacgtactga ggtacttttc cgtattctgg ttcttttttg cgtggttttt ttgtcttttc
    12601 catggtcttt gctaatgagg ttcaaaaata gcactttttt tcatatttag aaaaggtatt
    12661 tttgaacaat cctcattctg aattgttgaa aagttatacc tttgcactat tattctatat
    12721 atatgattac gtatccgaat gcaaaaataa atattgggtt gtttgttacc gaaaaacgaa
    12781 ccgatggtta ccacaattta gaaacgatat tttatcctat tccattaaaa gataaaatag
    12841 agtgtcatat ttcggataca aacgattctt cacttatcat atcaggtatc cgtatagatg
    12901 gcaatacctc agataattta gtaatgaaag catacaattt gcttaaatta tcgcacaact
    12961 taccaaatct taattttact ttagaaaaga atatcccttt tggtgcagga ttgggaggtg
    13021 gttcggcaga tgctgctttt gttcttacct tattaaatac gacctataat ttaggtttat
    13081 ccattgccga attagaatac attgcatcca agattggtgc agattgccct ttttttatac
    13141 agaataaacc agtattcgct acagggattg gtaatgagtt tcatcccatt tcattttctt
    13201 tgtctggtta tttccttgtt ttagtaaaac cgcctattca cgtatcaact ccgcaggcat
    13261 ataaaaatat caatcctcaa aaacctgatt ttgatttaaa aacaattcat ttactaccaa
    13321 taaaagaatg gaaacatgtt atttttaatg attttgaaac gaatgttttt attcaacacc
    13381 cagaaattga acagattaaa gatagtttgt accatcaggg agctttatat gcatccatgt
    13441 cagggtcagg ttcttctgtc tttggaatct tcccaaaaca ggtaaatatt acaaatttgt
    13501 ttcaaaattc cttcgtttgg tctggttttt tagcctaaaa gcctattttt cttctcattt
    13561 ttatcatatt atttgacgaa agttgtaaaa tatatgtttt acagcatata tttgtaatgc
    13621 attcgagagc gaatggctaa caaatgaata attaaaataa tgaaaggaga caaaattatg
    13681 acacagaatc agatttttga ctctttagtt ttagaaaatt aagggtcaat aaaatggaaa
    13741 tgattgctaa ccgcaagatt cgttacaaaa aagtgttgaa ccaattaaat ttacaactat
    13801 gtacaaagcg atggcatata aaggacttgg agatttaaaa ttatttgata ttatcttggg
    13861 acattcaagt tctgaaagaa aaataagcga aaagaaaaaa agtaaatagt gtttctaaat
    13921 gtcggaaaac tatataaatt ttaaatttta cagtcatgta taacacaata acatatagag
    13981 gaatagaaga cctaaagcta tttaatatac tttttggtca tatcaagaga gaaagaaaaa
    14041 acaagtaaat aatctaatga taaagattat taaacaacta aaataaataa tcatgtataa
    14101 cacaataaca tatagaggtg caaatgacct taaattgttc gatatattat ttggacatac
    14161 aactagcaga gaaaagaaac atcgtaaata agtagaagat tggcgataag tgttaaacta
    14221 gttaaaactt acaattatga aagaagaagg aatattatat aaaggaatgg aaaacatatt
    14281 taattttttt gattttatct ttggttatac aacccctgat aatgagtagt taataaataa
    14341 aaggagaaga aggctgagta gtatatactt agcctttttt ttcgttatac actaactgtt
    14401 tttttacttc tatgcctcct atataccctc ctaatttacc attgtggcaa ataactctat
    14461 gacaaggtat tactattaca aaaggatttt ttctacaggc atttcctatt gccctagcac
    14521 tgcttggttg accaattttt tcagctaatt ctttgtagct ataa
//
LOCUS       DAOSRP010000040        14390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4278_length_14390_cov_4.831740, whole genome shotgun sequence.
ACCESSION   DAOSRP010000040 DAOSRP010000000
VERSION     DAOSRP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4278_length_14390_cov_4.831740"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..2070)
                     /locus_tag="PLJ82_03780"
     CDS             complement(220..2070)
                     /locus_tag="PLJ82_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOR40658.1"
                     /translation="MNKATYSILCIVLLLTSCNLRDKEVIDPDYFFKNDSNLSLHTVV
                     ITDKATRLTFSILTESPTQIKIDSTAYLVANNKKYPLLENTLNVIAIDSGKYSFSLEF
                     EPLPKSTTSFDFIECEQESCIQMIGILLKGTYKDAEYKEKIPIQLVSANYSEAKMPKM
                     EMKCGSTQVIMHLVSPLPVDSSLFKGIISVFDITTGSYTDYQSNSDSLQTFSWEYEQC
                     SPGIVVLEIENNPIQLFVEPSKKNEFWIDVYAYFHQEPFVYTNGKLDALNRALHTRRN
                     DRYWIDTKELYPTFAEMNVATYLDFAKQTLIDSISSIEKNTSLSPLTRSLYIESEKSL
                     YIGRVLDANVIRYISANALNKNWETMELASYNQILAALEIIDFTSDNALYLMFKSGYH
                     FLIYTKSQYTENLFSTPSSALYQFKEYRKQYENLLVNYNHTPENSVDYLQSPFYSELF
                     RIKKIEQMQKSTQSVEIIDSLKKRKVYVEKTPITENENLLSNILSNYNGKVILINFWG
                     VTCGPCMFTIEKMEPLKKTELNNEKIVFVYLTTTNWSPIDEWFKKINTIQGHHYYLTT
                     DQWKYLNKQFHFNAIPFYVLVQKNGQFAPLKTGTDRSSSIKKSILEALNK"
     gene            complement(2231..3844)
                     /locus_tag="PLJ82_03785"
     CDS             complement(2231..3844)
                     /locus_tag="PLJ82_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR40659.1"
                     /translation="MITVSNLAIQFGKRTLFQEVNLKFTAGNCYGIIGANGAGKSTFL
                     RLLSGDLDPTKGSIHFGSNERLSVLKQDHHEFDEFTVLDTVLMGHTKLWDIKNEKDAI
                     YMKEDFSDEDGIRASELEEAFAELDGWNAESNAASLLSGLGIKEDLHYSLMKDISGKE
                     KVRILLARALFGNPDNLLLDEPTNDLDIDTINWLENYLANYENTVLVVSHDRHFLDAV
                     STHIVDIDYGKIQLVPGNYSFWYESSQLALRQQQAQNKKAEEKKKELEEFIRRFSANV
                     AKSKQATSRRKMLEKLNVEEIQPSTRKYPGIIFTPDREPGNMVLEVKGLSHSMEGQVL
                     FKDVNFNVEKGDKIVFLSKDPRAMTAFFEIINGNIKPDSGTFNWGVTITSAYLPLNNV
                     DFFNTDLNLVDWLGQFSDDTTESFIRGYLGRMLFSGEEIYKKAGVLSGGEKMRCMISR
                     MMLKNANVMVLDSPTNHLDLESIQAFNNSLKSFKGNILMSSHDHEFIQTVCNRVIELT
                     PNGIIDKVIEYDDYLDDMHVKTQREKLYK"
     gene            complement(3949..4716)
                     /locus_tag="PLJ82_03790"
     CDS             complement(3949..4716)
                     /locus_tag="PLJ82_03790"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOR40660.1"
                     /translation="MNYTELLHTFSEFPIVLALLLGLLVAIDPCPLAMNIGAIAFLGK
                     DIQDKRKVFINGLLYTLGRVLSYTVLGIVLLFVLQTTTNLHTLQTFFTSYGELLIGAI
                     FLVVGIFMLDLLKPHKHEHCDANHEVKTFTNKRNLSPFALGIVLALAFCPYGAALFFG
                     VLIPVSVESNVGYLLPILFAISTSIPVALMAWLLAFSVSNLNIINTKIHAYDKWIKRS
                     VSIILIVVGIYYVSSHFIGHTHDHHHHTEECTHDHEH"
     gene            complement(4799..5491)
                     /gene="ubiE"
                     /locus_tag="PLJ82_03795"
     CDS             complement(4799..5491)
                     /gene="ubiE"
                     /locus_tag="PLJ82_03795"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOR40661.1"
                     /translation="MAKKTYISGLFNGIASSYDTLNHILSLSVDKRWRKTAVKKVMQV
                     QPQKVLDVACGTGDFSIALAKAGVEKIIGIDIAEGMIAVGNKKVSQLQLSDKIELLYG
                     DSEHIAYENNTFDAVTVAFGVRNFENLEIGLKEMRRVINNNGLLVILELSVPTNKVLF
                     FFYNLYFKHIMPFIGGLISKDKSAYKYLYSSVIQFPKPHEFIQHLQNVGFSSIEHTAF
                     SGGLARMYVAKK"
     gene            complement(5495..5824)
                     /locus_tag="PLJ82_03800"
     CDS             complement(5495..5824)
                     /locus_tag="PLJ82_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOR40662.1"
                     /translation="MNTENNTPLLELENQIVRMLRTVFDPEIPVNIYDLGLIYKIEVS
                     DDNIVDIEMTLTAPSCPMADFILEDVKMKVEGIEGVKGLNINLVFDPPWDRNMMSEEA
                     QLELGFL"
     gene            complement(5808..6575)
                     /locus_tag="PLJ82_03805"
     CDS             complement(5808..6575)
                     /locus_tag="PLJ82_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR40663.1"
                     /translation="MTELNKEEEKFIVSLRRKFNKAIYQYGLISDGDVILVGLSGGKD
                     SLALVELLGERKKIFKPSFKVIAAHITLDNIPYTSDLAYLKAFCEQYDVPFVHRNSSF
                     DASTDTRKSPCFLCSWTRRKQLFDIAKELGCNKIALGHHQDDILETLLMNMSFQGAFS
                     TMPPILKMNKFDMTIIRPMCLLRENELIQLEKIRNYQKQVKNCPYEKDSFRKNMKQLI
                     QQIEKMNPEAHFSMWKAMTNIQADYLPKEIKENEYRK"
     gene            6702..8603
                     /gene="mutA"
                     /locus_tag="PLJ82_03810"
     CDS             6702..8603
                     /gene="mutA"
                     /locus_tag="PLJ82_03810"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOR40664.1"
                     /translation="MAEKKLNLLAEFPPVTTEKWMEKITADLKGADFSKKLVWKTNEG
                     FDVMPFYRAEDIETLKNTEAVPGKFPFVRGTKANNDWYVRQDIVVENVDEANKKALDV
                     LNKGITSLGFKISYQDLSSSYIQSLLKGIAADCVELNFSICTDHAAELGRLLVAYFQS
                     KGYDLVKLHGSINFDAMNAMLLRGKEYDKASIVSMAKAIIDAIAPLPFYRVVGVNATT
                     LSNAGAYIAQELGYALAWGNQYLSLLVEAGVDTSLAAKKIKFNFGVGGNYFMEIAKFR
                     AAKLLWALIVDAYQPICRRPNCDNTVNGICKCAARIRIHAQTSEFNKTVYDANVNMLR
                     TQTEAMSASLGGVDSLTVLPYDSVYKKSDDFSERIARNQQLLLKEESNFDKVADPASG
                     SYYIETLTNEIAQQAWKLFLEVEENGGFYAAIVAGNVQKAIKKTASTRLKNVSSRREV
                     LLGTNQFPNFNEQAITKVQKDNNASSCCTHNTSIEQLCAVRAADEFESLRFATEKATK
                     RPKAFMLTIGNLAMRLARAQFSCNFFACAGYDVVDNLGFKTIEEGIAAAKKANADIVV
                     LCSSDDEYAELAPQAFDLAKGNAILVVAGAPACTDELKAYGIEHFINVKSNVLDTLRT
                     FNGLLTINQ"
     gene            8606..9505
                     /locus_tag="PLJ82_03815"
     CDS             8606..9505
                     /locus_tag="PLJ82_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOR40665.1"
                     /translation="MSPTTKNWIIATRPWSFPASAMPALFAMAYVFYMQDSIESIHWG
                     YGVAALLGAVLFQASGNLIGDYFDYMYKVDRKESLGSSRMLVDGVFTPKTIFWFGIIV
                     LCMGILLGLFLWSQTGNDLLWIGIVGALGTFFYYKLKYMALGDVLIFVIYGPLIALGT
                     AFVMTNQLYWEVVVLSIPIAFLVVNILHANNTRDMRDDKLANIKTQALLLGEKGSRIQ
                     YTFLAIGAYVAVAIFVLLNMISPLALLVIVTLPLAKKNINLIKQAKIDKPEIIKDLDA
                     MSAQLVMAFTLMYALGIFNGYWI"
     gene            9502..10116
                     /locus_tag="PLJ82_03820"
     CDS             9502..10116
                     /locus_tag="PLJ82_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOR40666.1"
                     /translation="MKKVLLASLLAAVLWFIMFSPWTSIHINFWIAMACSAAILWAMS
                     AFLDKNFSKQFSFNVKDLVIGLVSAAVLYGVFVVGNYLSTQWFDFAGTQIQGIYGMKE
                     RQNLVLVGVLLTVLIGPAEEIFWRGYVQKQLGAKYGDWFAVLITVLIYTLVHIWSFNF
                     MLIMAAMVCGLFWGILYKYHKNIVTLIVSHAVWDVAVFILIPIA"
     gene            10193..12322
                     /gene="scpA"
                     /locus_tag="PLJ82_03825"
     CDS             10193..12322
                     /gene="scpA"
                     /locus_tag="PLJ82_03825"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOR40667.1"
                     /translation="MKPDFKSIDITNIAVSSGVGTQAESTWVTPEQIPVKPFYTKQDL
                     NGMQHLQYAAGLPPYLRGPYSVMYAMQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDADHERVVGDVGKAGVSICSIEDMKVLFDGIPLNKMSVSMTMNGAV
                     LPVLAFYINAGLEQGAKLEELSGTIQNDILKEFMVRNTYIYPPKFSMKIIADIFEYTS
                     KNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRAGVNAGMDIDSFAPRLSFF
                     WAIGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNV
                     GRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQQETDICREVDPW
                     AGSYYVESLTNELAEKAWALIEEVEKLGGMAAAIETGIPKMRIEEAAARTQARIDSGQ
                     QTIVGTNKYRLEKEDPLDILEVDNTAVRIQQIERLTQLKQARDNAAVKNALAAITKCV
                     ETGEGNLLELAVEAARVRATLGEISDACEVIVGRYKATIRTITGVYSAGTKNDADFMK
                     ACELTAKFAEKEGRQPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEA
                     AKQAVENDVHIMGVSSLAAGHKTLVPQVIAELKALGREDIIVIAGGVIPAQDYDYLYQ
                     AGVAAIFGPGTSVAKAAVTIMEILLED"
     assembly_gap    12433..12532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12546..12698)
                     /locus_tag="PLJ82_03830"
     CDS             complement(12546..12698)
                     /locus_tag="PLJ82_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40668.1"
                     /translation="MHKTILSGIVHEIPEDIAKILLENDAILDCWPGCPHRRSSAQKW
                     FTNKSM"
     gene            complement(12691..13374)
                     /locus_tag="PLJ82_03835"
     CDS             complement(12691..13374)
                     /locus_tag="PLJ82_03835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR40669.1"
                     /translation="MNVLFEDNHIIAINKSCSDIVQGDKTGDTPLVEKVKIYLKEKYN
                     KPGNVFCGVTHRLDRPVSGIVLFAKTSKALTRLNEMLREREIKKTYWAITKNRPAKPE
                     MLLEHYLLRNEKQNKSYAYDTPKGDAKKATLTIKELATSTNYTLLEVDLQTGRHHQIR
                     CQLAHIGCPIKGDLKYGFARSNPDGGISLHARQINFIHPVTKEPIEIIAPTPEDNLWK
                     AFEDMIRNA"
     gene            complement(13379..14116)
                     /gene="rlmB"
                     /locus_tag="PLJ82_03840"
     CDS             complement(13379..14116)
                     /gene="rlmB"
                     /locus_tag="PLJ82_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR40670.1"
                     /translation="MKKESEMIYGMHAIIEAIDAGKEIDKIIMKNDMQGRLSHDLFKA
                     IEGRNIPIMRVPVERLDRITKKNHQGVIAFTIPVAYQHIEDIIPTLYEDGKAPFIIVL
                     DGITDVRNFGAIARTCECAGVHAIVIPEKNSASINADAIKTSAGALLKIPVCREKNLT
                     NAIKFLKNSGLKIVATSDKADDNYTDISYTDALAIVMGAEDIGISIENQRLCDTIVKI
                     PIVGTISSLNVSVATGIMAYEVLKQRL"
     gene            complement(14154..>14390)
                     /locus_tag="PLJ82_03845"
     CDS             complement(14154..>14390)
                     /locus_tag="PLJ82_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOR40671.1"
                     /translation="APPFRKAEFDIMFGEGISIAGEIIDLGVEQGIIKKSGSWFSYGD
                     TKLGQGRDAAKKVMIDNPELAAELEQKIMEALAK"
BASE COUNT         4361 a         2421 c         2687 g         4821 t
ORIGIN      
        1 actattcagg tagtgaagta aattatttat attctttcaa tctgaatcat ccagagtttg
       61 ctatagagga agttggagag taagtaggat tctctggttc gacgtaggct gtgcctacgt
      121 cgtgctaaaa gcaaggattt accttgcata aacaatatta gcaatagaaa acagacattt
      181 gaagtagaga atcttttcct tgttctcgaa aactcaaaat tatttattta aagcctccaa
      241 gatcgatttt ttaatactgc ttgatcggtc ggttcctgtt tttaatggag caaactgccc
      301 gtttttttgt actaaaacat aaaatgggat agcattaaaa tggaattgct tgtttaaata
      361 tttccattga tcagttgtaa ggtaatagtg atgaccttga atagtattga tttttttaaa
      421 ccattcatca attggagacc agttcgtcgt agttaaatag acaaatacaa ttttctcgtt
      481 atttaactcc gtttttttta agggttccat cttttcaatt gtaaacatac atggtccaca
      541 agtaactccc caaaaattta ttaaaatcac ttttccatta taatttgaca aaatatttga
      601 tagtagattt tcattttcag ttataggagt tttttctaca tatacttttc ttttttttaa
      661 tgaatcaatt atttctacag attgagttga cttttgcatt tgctcaattt tttttattcg
      721 gaataattca gaatagaagg gtgattgcaa gtagtcaaca ctgttttcag gtgtatgatt
      781 gtaattaact aataaattct catattgttt tctgtattct ttaaattgat aaagggcaga
      841 agatggagta gaaaataagt tttctgtata ttgtgacttt gtataaatta aaaaatgata
      901 tcctgattta aacatcaaat acaaggcatt gtcagaagta aaatcaatta tttctaaagc
      961 agcaagtatt tgattataag atgcaagttc cattgtttcc caatttttat ttaaagcatt
     1021 tgctgaaata tagcgaataa catttgcgtc aagcacacga ccaatgtata gagatttctc
     1081 agattcaata tacaaagaac gagttagagg agatagcgat gtgttctttt ctattgaaga
     1141 gatagaatct ataagagttt gttttgcaaa atctaaatat gtagctacgt tcatttcagc
     1201 aaaagttgga tatagttctt ttgtatcaat ccaataccta tcgtttcttc tagtatgtaa
     1261 cgcacggttt agtgcgtcta atttcccatt agtatataca aaaggttctt gatgaaagta
     1321 ggcatatacg tcaatccaaa attcattctt cttactgggt tcaacgaata gttgaattgg
     1381 attgttttct atttctaata ctactattcc aggactacat tgctcatatt cccaactgaa
     1441 tgtttgtaaa gaatcactat ttgattggta gtcagtatac gaccctgttg ttatgtcaaa
     1501 aacagatatt attcctttaa acagcgaaga atcaacaggt agtggactta ctaagtgcat
     1561 tataacctgc gtactaccac atttcatttc catttttggc attttagctt ctgaataatt
     1621 ggcagaaact aactgtatgg gtattttttc cttatactca gcatctttgt aggtcccttt
     1681 taacaaaatt ccaatcattt gtatacaact ttcctgctca cattcaataa agtcaaagga
     1741 tgttgttgat tttggtaacg gttcaaattc tagcgaaaaa gaatacttgc cagaatcaat
     1801 tgctataaca tttagcgtat tctctaaaag agggtatttc ttgttatttg caactaaata
     1861 cgccgttgaa tcaattttta tttgtgttgg cgactcagtg agtattgaaa aagttagacg
     1921 tgttgcttta tctgtaatta ctactgtgtg taatgaaaga ttgctatcgt ttttaaaaaa
     1981 ataatcaggg tctattactt ctttatctcg taggttacaa ctagttaata ataggacaat
     2041 acacaatatg gaatatgttg ctttgttcat agagttttag tattaaattt caaaaaaaaa
     2101 tattgtttgt gtctttcaac ttacttctat aaacgtgaaa agagtaataa tagtatatcg
     2161 attattaaaa taataaaaaa acctcctaaa caaagtagta taggaggttt tattgtttgt
     2221 tacaacaaat ttatttgtat aatttttctc gttgagtttt tacgtgcata tcgtctaagt
     2281 aatcatcgta ttctataact ttatcaataa tgccattggg tgtaagttca attacacggt
     2341 tacatactgt ttgaataaac tcatggtcgt gagacgacat caggatattt cccttaaatg
     2401 attttagcga gttgttaaat gcttgaatcg attccaaatc caagtggttg gtaggagagt
     2461 cgagcaccat aacattggca tttttcagca tcatacgcga aatcatacaa cgcatttttt
     2521 ctcctcctga taatacgcct gcttttttat aaatttcttc gccagaaaac aacattctgc
     2581 ccaaatagcc acgtataaat gattcggtag tatcgtcaga aaattgtccc aaccaatcta
     2641 ctaaattcaa atcagtatta aaaaaatcaa cattattaag cggtaaatag gctgaggtaa
     2701 tggttactcc ccagttaaat gttccactgt ctggttttat attgccattg ataatctcga
     2761 aaaacgcagt catagcccta gggtctttgg ataaaaatac aattttatcc cctttttcta
     2821 cgttaaagtt tacatcttta aacaatactt gaccttccat gctatgcgaa agacctttca
     2881 cctccagtac catattgcct ggttcgcgat cgggtgtaaa aatgatacca gggtatttgc
     2941 gggtagaagg ttgaatttct tctacgttca gtttttcaag catttttcta cggctagtcg
     3001 cttgtttgga tttagccacg ttggcactaa aacgacgaat aaactcttcc aattcctttt
     3061 tcttttcttc cgctttcttg ttttgtgctt gttgctgacg caaagccagc tgactagatt
     3121 cgtaccagaa agaatagtta cctggtacta attgtatttt gccataatca atatccacaa
     3181 tatgggtact tacagcatct aaaaagtgac ggtcgtgcga tactaccaaa acggtatttt
     3241 cgtagtttgc taaataattt tctaaccagt tgatggtgtc aatatccaaa tcgttggtag
     3301 gctcatcaag caataggttg tctgggtttc caaacaaagc ccttgccaac aatatacgta
     3361 ctttttcttt tccactaatg tctttcatta gcgagtagtg caagtcttct tttataccca
     3421 aaccactcag tagcgatgct gcattgcttt cggcgttcca tccgtcgagt tcggcaaaag
     3481 cctcttctag ttcagatgct cttataccat cttcgtccga aaaatcttct ttcatgtaaa
     3541 tagcatcttt ctcgttttta atatcccaca acttggtgtg tcccattaaa acggtgtcaa
     3601 gtacagtaaa ttcatcaaat tcgtggtggt cttgttttaa tacagataaa cgttcgtttg
     3661 aaccgaaatg aatggagcct tttgttgggt ctaaatcgcc acttaacaaa cgtaagaagg
     3721 ttgattttcc tgcaccatta gcacctatta tgccataaca gtttccggca gtaaatttta
     3781 aattaacttc ttgaaataaa gtgcgtttgc caaattgaat ggccaaattt gaaactgtaa
     3841 tcatgtctat tgtaagtttg ttttattttt gcgaccgcaa agataggcaa ttttctttag
     3901 atttaaaaga aaagtgtagc tgtcagaaaa tcaaatagag ataatagctc aatgctcgtg
     3961 gtcatgcgta cattcttcgg tgtggtggtg gtgatcgtga gtatgaccta taaaatggct
     4021 tgatacataa taaattccta caacaattaa aataatggaa acactacgtt taatccattt
     4081 atcgtatgca tgaatttttg tgtttattat attcaaatta gaaacgctaa atgccaataa
     4141 ccaagccatt aaagcaacag gaatgctggt gcttatagca aacaaaatgg ggagtaagta
     4201 gccaacgttt gattcgactg aaacaggaat taacacccca aaaaagaggg cagcaccata
     4261 cggacaaaat gccaatgcta atacaatgcc tagtgcaaaa ggacttaaat ttcgtttatt
     4321 cgtaaatgtt tttacttcgt gattagcatc acaatgttcg tgtttgtgcg gttttaacaa
     4381 gtctaacata aaaataccta caactaaaaa tatggcacca atgagcaatt cgccatacga
     4441 agtgaaaaag gtttgcaagg tgtgtaaatt ggtggtggtt tgtagcacaa ataataatac
     4501 aatgcctaat actgtatagc tcaacaccct accaagggta tatagtaagc cgttgataaa
     4561 tacttttcgt ttatcttgaa tatcctttcc caaaaaggca atagcaccaa tattcattgc
     4621 caaagggcag gggtctatgg ctacaagcaa cccaagtagc aaggctaata caataggaaa
     4681 ctcagaaaat gtatgtagaa gttcagtgta gttcacggtt gtgtttattt ttacgcaaaa
     4741 atacaactaa gtttgagaat ggagctttct tatttcctaa aaaactaaaa taagctgatt
     4801 atttttttgc cacatacata cgagctaatc cgcccgaaaa ggcagtatgt tcaatactcg
     4861 aaaaaccaac attttgtaaa tgctggataa attcgtgtgg tttaggaaac tgtataaccg
     4921 acgaatataa gtatttatag gcggatttat ctttggatat cagtcctcca ataaaaggca
     4981 taatatgttt aaaataaagg ttgtagaaaa agaataacac tttattggta gggacagaca
     5041 attcgagaat gactaataat ccattattat taataactct tcgcatttct tttaagccaa
     5101 tttcaaggtt ttcaaaattg cgaactccaa aggctacagt tacagcatca aaggtgttgt
     5161 tttcgtatgc aatatgctca ctgtcaccat acaaaagctc tattttatcg gataattgta
     5221 gctgcgaaac ttttttgttg cctacagcta tcataccctc agcaatgtcg atgccaataa
     5281 ttttttcaac acctgctttg gctagtgcta tcgaaaaatc tcctgttcca cacgccacat
     5341 ccaatacttt ttgtggttgt acctgcatta cttttttaac agccgttttt ctccaacgtt
     5401 tatctaccga caacgataaa atatggttaa gcgtatcgta gcttgaggca atcccattaa
     5461 ataaacccga aatatacgtt ttttttgcca taccttacaa aaatcctagt tctaattgtg
     5521 cttcttccga catcatgttt ctgtcccaag gtggatcaaa aacaaggttg atattcaatc
     5581 ctttaacacc ttcaatgcct tctactttca tttttacatc ttctaaaata aaatcagcca
     5641 tagggcagct tggtgctgtt aaggtcattt caatatctac gatgttgtca tcacttactt
     5701 ctatcttgta aattaaacct aaatcgtaaa tatttactgg aatctctggg tcaaatacgg
     5761 tacgcaacat gcgtacaatc tggttttcta attccaatag cggagtgtta ttttctgtat
     5821 tcattttctt taatttcttt tggtaaataa tcagcttgta tattcgtcat agctttccac
     5881 atcgaaaaat gagcttctgg gttcattttt tctatttgtt ggataagctg tttcatattt
     5941 tttctaaatg agtctttttc atagggacaa ttctttactt gcttttgata gtttctgatt
     6001 ttttcaagtt gtatcaattc attttctcgt agcaaacaca tcggtcgtat aatcgtcata
     6061 tcaaacttgt tcattttcaa tataggaggc atggtcgaaa atgctccttg aaaactcata
     6121 ttcatcaaca atgtttccaa tatatcgtct tggtgatgcc ccaatgcaat tttgttgcaa
     6181 cccaactctt tggcaatatc aaaaagctgt tttctgcgtg tccacgaaca caaaaagcaa
     6241 ggtgattttc gggtgtcagt actggcatca aaactactat tgcgatgaac aaaaggtaca
     6301 tcatattgtt cgcaaaacgc ttttaaataa gccaaatctg atgtataagg aatattatcc
     6361 aatgtaatat gtgctgcaat taccttaaaa gaaggcttaa atattttttt tcgctcacct
     6421 aataattcta ccaatgccaa tgaatctttc cctcccgaaa gccccactaa aataacatct
     6481 ccatctgaaa ttaaaccgta ctgatagata gctttattga atttccgtct taacgaaacg
     6541 ataaattttt cttcttcttt gtttaattca gtcattttga atgctatttt cacaacaaaa
     6601 gtacttattt tattaaaatt tacaagccta aaaattcatt atattaatta aaacaactaa
     6661 ttttgctgag ttttagaaaa ttatacaatt attatattat tatggcagaa aaaaaattga
     6721 atttattggc tgaatttcca ccggtaacaa ccgaaaagtg gatggagaaa attacggctg
     6781 acttgaaagg tgctgatttt agtaaaaaat tagtttggaa aaccaatgag ggttttgatg
     6841 taatgccgtt ttatcgtgca gaggatattg agactttgaa aaacacagag gctgttccag
     6901 gaaagtttcc gtttgtaagg ggtacaaagg ctaacaatga ttggtatgtt cgtcaggata
     6961 ttgtagtaga aaatgtggat gaggcgaaca aaaaagcatt ggatgttctt aacaagggaa
     7021 ttacttcgct tggttttaaa attagctatc aagatttgtc aagtagctat atccaatcac
     7081 ttttaaaagg tattgctgct gattgtgtag agttaaattt tagtatttgc accgatcatg
     7141 ctgctgaatt aggtcgttta ttggtagcct attttcagtc aaaaggatat gacttagtta
     7201 aattgcatgg ctccatcaac tttgatgcta tgaatgctat gttattgcgt ggtaaagaat
     7261 atgataaagc aagtatcgta tcgatggcaa aagcaattat tgatgcaatt gctcctcttc
     7321 ctttttaccg tgttgtgggt gttaatgcta caaccttaag caatgctgga gcttatatag
     7381 ctcaagagtt aggatatgca ttggcttggg gaaatcaata tctttcatta ttggtagagg
     7441 caggtgtaga tacatcgttg gctgctaaaa aaattaaatt taattttggt gtaggtggca
     7501 attattttat ggaaattgcc aaattccgtg ctgccaaatt gttgtgggca ttgattgtag
     7561 atgcatacca accaatttgt cgtcgtccta attgtgataa tacagtaaat ggtatttgca
     7621 aatgtgctgc cagaatacgc attcatgcac aaacatcaga atttaacaaa acagtatatg
     7681 atgccaatgt aaatatgctt cgcacacaaa cggaagctat gagtgcttcg ttgggtgggg
     7741 tagattcgct aaccgtattg ccttacgatt ctgtgtacaa aaaatcagat gatttttcag
     7801 aacgcattgc tcgtaaccaa caattattat tgaaagaaga atcaaatttt gataaggtgg
     7861 ctgacccagc ttctggttcg tactatatcg aaacattaac caatgaaata gctcaacaag
     7921 cttggaaatt atttttagaa gtagaagaaa atggtggttt ttatgcagct attgtagcag
     7981 gtaatgtaca aaaagcaatt aaaaaaactg caagtacgcg tttaaaaaat gtttcatctc
     8041 gccgtgaagt gttacttgga acaaatcaat ttcctaattt caacgagcaa gcaattacta
     8101 aagttcaaaa agataataat gcatcatctt gttgcacaca taatacatct atcgaacagt
     8161 tatgtgcagt acgtgcagct gatgaatttg aatccttacg ttttgctacc gaaaaagcga
     8221 ctaagcgtcc aaaagcattt atgctaacca ttggtaactt ggctatgcgt ttggcaaggg
     8281 ctcagttttc gtgcaatttc tttgcttgtg cagggtatga tgtagtagat aatttaggat
     8341 ttaaaaccat cgaagaaggt atagcagcgg ctaaaaaagc caatgctgat atagtggtat
     8401 tatgctcaag tgatgatgaa tatgctgaat tagcccctca agcttttgat ttggcaaaag
     8461 gaaacgctat tttagtagta gcaggagctc cagcttgtac tgatgaatta aaagcctatg
     8521 gtattgagca ttttatcaat gttaaatcca atgtgttgga tactttacgt acgtttaacg
     8581 gcttattaac tatcaatcaa tagagatgag tccaactaca aaaaattgga taatagctac
     8641 acgcccatgg tcgtttccgg catcggcaat gcccgctttg tttgctatgg cgtatgtttt
     8701 ttacatgcaa gatagcatag agtccatcca ttgggggtat ggagtagctg ctttattggg
     8761 tgctgtcttg tttcaagcaa gcggaaatct tattggcgat tatttcgatt acatgtataa
     8821 agtagataga aaggaatcgc tcggttcgag ccgtatgttg gtagatggtg tatttacgcc
     8881 caaaacaata ttctggtttg gaataatcgt attatgtatg ggtattttgc tcggtttgtt
     8941 tttatggtcg caaacaggca acgatttgct atggattggt atcgttggtg ctttgggtac
     9001 atttttttat tacaaactaa agtatatggc tttgggcgat gtcttgattt ttgttattta
     9061 cggaccgctc attgctttag gaacagcctt tgtaatgacg aatcaattat attgggaggt
     9121 tgtagtgtta agtattccga ttgctttttt ggttgtgaat attttacatg caaacaacac
     9181 acgtgatatg agagatgata aattggcaaa tatcaaaaca caagccttgt tgcttggcga
     9241 aaaaggttcg cgcattcaat ataccttttt agctattgga gcgtatgtgg ctgtggcaat
     9301 atttgtatta ttaaatatga tatcgccttt ggcattgttg gtaatagtaa ccttgcccct
     9361 tgcaaaaaag aatatcaatt taataaaaca agctaagatt gataaacctg agattatcaa
     9421 agatttagat gctatgtcag ctcaattggt gatggcattt acattgatgt atgctcttgg
     9481 aatttttaat ggatattgga tatgaaaaaa gtattattag cgtcattgct tgctgctgtt
     9541 ctgtggttta ttatgttttc gccatggaca tctatccata ttaatttttg gatagcaatg
     9601 gcttgttcag ctgcaatttt gtgggctatg agtgcttttt tggataaaaa ttttagcaaa
     9661 caattctctt tcaatgttaa agatcttgta ataggactgg tgtctgctgc tgttttgtat
     9721 ggggtgtttg tagtgggtaa ttatttatct acccaatggt ttgattttgc tggcactcaa
     9781 atacaaggca tttatggaat gaaagaaaga caaaatttgg tattagtagg cgtattactt
     9841 actgtgctga ttggtcctgc tgaagaaata ttttggcgtg gatatgtgca aaaacaactt
     9901 ggtgcaaaat atggcgattg gtttgctgta ttaatcactg tgttaattta taccttagta
     9961 catatctggt cgtttaattt tatgttaatt atggcagcca tggtgtgtgg attgttttgg
    10021 ggcatattgt acaaatacca caaaaatatt gtaacactca ttgtgtcgca tgctgtttgg
    10081 gatgtagcgg tatttatcct aattccaatt gcataaataa ttgagaaata ataaaaatga
    10141 ataataaaat tgagtacatt cctgaatggc tcataaatta aaaaaaatga acatgaaacc
    10201 tgattttaaa agtattgaca ttacaaacat cgctgtttca tcaggcgttg gtacacaagc
    10261 ggaaagtact tgggtaacac ctgagcaaat tcctgtgaaa ccgttttaca caaaacaaga
    10321 cctaaatggt atgcaacatt tacaatatgc tgcgggttta cctccttatt tacgtggacc
    10381 ttatagtgta atgtatgcta tgcaaccttg gactattcgt cagtatgcag gattttctac
    10441 tgcagaagaa tcaaatgcgt tttaccgtag aaatttagct gctggtcaaa aaggattgtc
    10501 tgttgctttc gacttagcta ctcaccgtgg atacgatgcc gaccacgaga gggtagttgg
    10561 tgatgtgggt aaagctggtg tatctatctg ttcaatcgaa gatatgaaag tgttgtttga
    10621 tggtattcca ttgaataaaa tgtctgtatc aatgacgatg aatggtgctg tattgcctgt
    10681 gttagctttt tatatcaatg caggattaga gcaaggtgct aaacttgaag aattgtcagg
    10741 tactatccaa aatgatattt tgaaagaatt tatggtgcgt aatacgtata tttatccacc
    10801 taaattttcg atgaagatta ttgctgatat ttttgaatat acgtcaaaaa atatgcctaa
    10861 attcaactca atttctattt cgggatacca catgcaagaa gctggtgcta ctgctgatat
    10921 tgagttagca tataccttgg cagatggatt ggagtactta cgtgcgggtg taaatgcagg
    10981 tatggatatc gattcgtttg ctcctcgttt atcatttttc tgggcaatag gcatgaatca
    11041 ctttatggaa attgctaaaa tgcgtgcagc tcgtttgcta tgggcaaaaa ttgtaaaaca
    11101 atttaatcct aaaaacccaa aatctttggc tttgcgtacc cactcgcaaa cttctggatg
    11161 gtcgctaaca gagcaagatc catttaataa cgttggtcgt acgtgtattg aggcaatggc
    11221 ggctgccttg ggacatacac aatcattgca caccaatgca cttgacgagg ctattgcttt
    11281 accaacagat ttttcggctc gtattgctcg taacacacaa atttatattc aacaagaaac
    11341 ggatatttgt cgcgaagttg acccttgggc aggttcgtat tacgtagagt ctttaacgaa
    11401 tgaattggct gaaaaagcgt gggctttaat tgaagaagtt gagaaattgg gtggtatggc
    11461 tgctgctatc gaaacaggta ttccaaaaat gcgtatcgaa gaggctgctg ctcgtaccca
    11521 agctcgtatc gactcgggtc agcaaaccat tgttggaacc aacaaatacc gtttagaaaa
    11581 agaagatcct ttggatattt tagaagttga taatacagcc gtacgcatac aacaaattga
    11641 gcgtttaact caattgaaac aagccagaga taatgccgct gtaaaaaatg ccttagctgc
    11701 cattaccaaa tgtgtagaaa caggcgaagg aaacttgttg gagttagctg tagaagcagc
    11761 tagagtgcgt gcaacattgg gcgaaatctc agatgcatgc gaagtgatag taggaagata
    11821 taaagcaacc attagaacca taacaggagt gtatagtgca ggaacaaaaa atgacgcaga
    11881 ctttatgaaa gcgtgcgaat taacagcaaa atttgctgaa aaagaaggtc gtcagccacg
    11941 tattatgatt gccaaaatgg gtcaagacgg acacgaccgc ggtgcaaaag ttgtagccac
    12001 agggtatgcc gattgtggtt ttgatgtgga tatgggacct ttgttccaaa cgccagccga
    12061 agcagccaaa caggctgttg aaaatgacgt acacataatg ggcgtttcat cattagctgc
    12121 aggtcacaaa accttagtac cacaagttat tgctgaacta aaagccttgg gacgtgaaga
    12181 tatcattgta atagcgggtg gggtaattcc tgcacaagat tatgattatc tatatcaagc
    12241 aggtgttgct gccatctttg gtcctggaac atcggttgca aaagctgctg ttactattat
    12301 ggagattttg ttagaagact aagatagttt tttactttat aaaaaaacga ggttgtaatt
    12361 atgtgttaca acctcgtttt tgttttctga ttacatagat ttgttagtaa accatttttg
    12421 agcagatgaa cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttgttt
    12541 tctgattaca tagatttgtt agtaaaccat ttttgagcag atgaacgcct gtgtggacat
    12601 cctggccaac agtcaagaat agcatcattt tcaagcaata tttttgctat atcttcagga
    12661 atttcatgta ctatgcccga aagaattgtt ttatgcattt cttatcatgt cttcaaaagc
    12721 cttccaaaga ttatcttctg gtgttggggc aataatctca attggttctt tggttactgg
    12781 atgtataaaa tttatttgtc gggcgtgcag gctaatacct ccatcaggat ttgagcgtgc
    12841 aaagccatat tttaagtccc cttttattgg gcaaccgata tgtgctaatt gacaacgaat
    12901 ttggtgatgt ctgcctgttt gtaaatctac ttcaagtaat gtatagttgg tgctggtagc
    12961 caattcttta attgtaagag ttgctttttt tgcatcgcct ttaggcgtat cataggcata
    13021 cgatttattt tgcttttcgt tgcgaagtag gtagtgctct aatagcattt ctggtttagc
    13081 tggtcggttt tttgtaatag cccagtaggt tttttttatt tcgcgttcgc gtaacatttc
    13141 gtttaatcgg gtaagagcct tgctcgtttt agcaaatagc acaataccac tcacaggacg
    13201 atcgagtcga tgagtaacac cgcaaaatac attgccaggt ttattgtatt tttctttgag
    13261 gtaaattttc accttttcaa ccaaaggagt atcacccgtt ttatcgcctt gtacaatatc
    13321 tgaacacgat ttgttgatag caattatatg gttatcttcg aatagtacat tcatcttctt
    13381 acaagcgttg tttcagtact tcatacgcca taataccagt ggctacagat acatttaaag
    13441 acgaaatagt gcccactatc ggaattttaa ctatagtatc gcacaaacgt tggttttcga
    13501 tagaaatacc tatatcttca gcccccatta caatagcaag tgcatctgtg tatgatatat
    13561 ccgtataatt atcatcagct ttatctgatg tagccacaat ctttagacca gagtttttaa
    13621 ggaattttat ggcattggtc aagttttttt ctcgacaaac aggtattttg agtaatgctc
    13681 cagccgaagt ttttatggca tctgcattga tagaagcact atttttttcg ggaataacaa
    13741 tggcatgtac acctgcacat tcgcaggtac gtgctatggc tccaaaatta cgtacatcgg
    13801 ttataccgtc aagtactata ataaatggtg cttttccatc ttcatagagg gtagggataa
    13861 tatcttcgat atgttgatag gcaaccggta ttgtaaaagc aattactcct tgatggtttt
    13921 tcttggtaat tctgtctaag cgttcgacag gaacacgcat aatgggtata tttctaccct
    13981 caattgcctt aaacaagtcg tgagacaagc gtccttgcat atcatttttc atgataattt
    14041 tatcaatttc cttgccggca tcaatggctt cgataatggc atgcatgcca tatatcattt
    14101 ccgattcttt tttcatgtgt gttctttgcg taaatgtgtg ttgatatgga actttatttt
    14161 gctaaagctt ccattatttt ttgttccaat tcggcagcta attctggatt gtctatcatt
    14221 acttttttag cagcatctct accttgtcct agtttggtgt cgccgtagct aaaccacgat
    14281 ccgctttttt taataatgcc ttgttcaact cctaaatcaa taatttctcc tgcaatagaa
    14341 attccttcac caaacatgat atcaaattca gctttacgga aaggaggagc
//
LOCUS       DAOSRP010000041        14360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4289_length_14360_cov_4.965956, whole genome shotgun sequence.
ACCESSION   DAOSRP010000041 DAOSRP010000000
VERSION     DAOSRP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14360
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4289_length_14360_cov_4.965956"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..545)
                     /locus_tag="PLJ82_03850"
     CDS             complement(150..545)
                     /locus_tag="PLJ82_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40672.1"
                     /translation="MTVGAVTLPSSSFVPSGGSGDGGYDPISIDPIQSVPSYSYTALG
                     ASSCPTKYPKSEICYDCCSYEMITCLYGGNYAACHAAMIECNLACDRTYSLPIGDAIP
                     FMLLLIVLYGIIRYRKNMAFRVINNQKGE"
     gene            complement(698..1507)
                     /locus_tag="PLJ82_03855"
     CDS             complement(698..1507)
                     /locus_tag="PLJ82_03855"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR40673.1"
                     /translation="MSNIQAKYVVVVAGGKGKRMQSEMPKQFLLLHGKPILMHTLETF
                     FLYDHSIQIILVLPEDDIDMWKKLCQQHAFELTHTIVNGGNERFYSVANGLTLVPEGV
                     LVAIHDGVRPFPSTSTIERTFGMAAEKGNAIPVVELTDSIRQLSDNQENKSVSRAAFR
                     LVQTPQTFQSTQIKKAYTQSYSPLFTDVEDTELVGESISIFAQNGSVIISSTEGVALQ
                     TVYVSDMAGKTDTYKLSGSHYQKLDLPVAKGVYLISVVGDTMTKEQKVIIK"
     gene            1622..2083
                     /locus_tag="PLJ82_03860"
     CDS             1622..2083
                     /locus_tag="PLJ82_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOR40674.1"
                     /translation="MAKQVLFSILFGFLMAIQGMAQENPLENDSLLQEETILSEHDRM
                     DTILYRAKNTATSEIEFVSIGGFRVQVYAGNHPQKAREDAMLIDKTIKEQYPEYHAYV
                     SFQAPFWRVRIGDFASYYEALLLSRELKKIFPDFGNEIYVVKDPTVRILYF"
     gene            2087..2674
                     /gene="folE"
                     /locus_tag="PLJ82_03865"
     CDS             2087..2674
                     /gene="folE"
                     /locus_tag="PLJ82_03865"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOR40675.1"
                     /translation="MEKEKPASSPEILQELASHYKAVIELIGENPTREGLLKTPERMA
                     KSMQFLMRGYNQDPETIIRSAMFTEDYRQMVIVKDIDFYSMCEHHMLPFFGQAHVAYI
                     PNKKITGLSKIARVVDVFARRMQVQERLTTQIKECIQNTLNPLGVMVVIEAQHMCMQM
                     RGVEKQHSVTTTSDFTGIFEQAKTREEFISLISKK"
     gene            complement(2731..3483)
                     /gene="lptB"
                     /locus_tag="PLJ82_03870"
     CDS             complement(2731..3483)
                     /gene="lptB"
                     /locus_tag="PLJ82_03870"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOR40676.1"
                     /translation="MLSENQMVLRTEHLVKRYKTRTVVDNVSISVKQGEIVGLLGPNG
                     AGKTTTFYMTVGLVTPNQGRIFLNDLDISSYPVYKRAQQGIGYLAQENSVFRKMSVED
                     NILSVLQMTKFSKKYQHEKLETLISEFSLQTVRKSLGDQLSGGERRRCEIARCLAIEP
                     KFIMLDEPFAGVDPIAVEDIQRIVAQLREKNIGILITDHNVHETLSITDRAYLLFEGK
                     ILFQGTAEELADNPVVREKYLGASFELRKKVE"
     gene            complement(3487..4566)
                     /locus_tag="PLJ82_03875"
     CDS             complement(3487..4566)
                     /locus_tag="PLJ82_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOR40677.1"
                     /translation="MNYNNYCVIMAGGVGSRFWPFSKNSMPKQFLDFFGTGRTLLQMT
                     FDRFAKVVPASNILIVSNKDYKDIILEQLPLIKPSQVLLEPNRRNTAPCVAYAVSRIK
                     AINPKANMVVAPADHLIIKEIDFLNVIERSLNFVNEHPNLLTLGIKPNRPETGYGYIQ
                     ISDQAMGDFKKVKTFTEKPNLELAKVFVESGEFFWNSGVFVWSVEAIWKAFNEFLPEM
                     VAKFEAGSDKFNTDLEQDFINEIFPSCQNISIDYGIMENAKNVYVLCGDFGWSDLGTW
                     GSLYDMSEKDEHQNVTLKCKTQFYESNDNIVVLPENKLAVIQNLSGYIVVESDNVLLI
                     CKKEDEQRIRHFVTDVNLKYGEGYI"
     gene            4783..4866
                     /locus_tag="PLJ82_03880"
     tRNA            4783..4866
                     /locus_tag="PLJ82_03880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4817..4819,aa:Leu,seq:caa)
     gene            complement(4911..5699)
                     /locus_tag="PLJ82_03885"
     CDS             complement(4911..5699)
                     /locus_tag="PLJ82_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOR40678.1"
                     /translation="MNYLLPKQLVSVASDAAEMRAKTTVSKTLVLAFLAGAYIAIGGL
                     LAVMIGAGVPGIAEHNPGIPKFLMGAVFPIGLIMCAIAGADLFTGNTAYFIPPVMSGK
                     LQITDLLRNWTLVYVGNFIGALFVAYAMTYLTGLFDNDPWNAGIMHIAEVKTANPFYK
                     TFLKGIGANWLVALAMWLSFASKDVVGKIVAIWFPVMAFVTLGFEHSIANMFFIPTAI
                     LYGADVSWASFIFDNLIPATLGNIVGGGLLVGMAYWYVYEKEEK"
     gene            complement(5729..6454)
                     /gene="pflA"
                     /locus_tag="PLJ82_03890"
     CDS             complement(5729..6454)
                     /gene="pflA"
                     /locus_tag="PLJ82_03890"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOR40679.1"
                     /translation="MINVHSVESLGTYDGPGIRLVLFLQGCNFKCLYCANPDTIEPKT
                     GRPYGADDILKMARNQRPFFGKKGGVTVSGGEPLLQARALIPIFKALKEDGFNTCIDT
                     NGSVLTDAVKELLTYTDLVLLDIKQMDEEAHQAITGVKNHLTLRFATYLAEIGKPTWI
                     RYVLVPGYTDQQEHLHQLGAFVKDMKNVEKIEIQPYHKLGKHKYEALGWQYQLEDVEM
                     NTAEQLTKAKKILSTYGKEVVVN"
     gene            complement(6472..8688)
                     /gene="pflB"
                     /locus_tag="PLJ82_03895"
     CDS             complement(6472..8688)
                     /gene="pflB"
                     /locus_tag="PLJ82_03895"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOR40680.1"
                     /translation="MNFKNGKWNNAVDVRDFVSLNITPYDGDSGFLCSPTQRTLDLWQ
                     ICKDAISEERANNGVRSIDNELVSTITSHAPGYIDKEKELIVGLQTDQLLRRAMKPYG
                     GIAVVEKACSENGVEVSPFVKEVFTKYAKTHNDGVFDVYTPEIRTFRSLGVLTGLPDN
                     YARGRIIGDYRRVALYGVNFLIQQKKKDLESLLGPMSDERIRLREEVSEQIKALYAII
                     EMGKSYGFDLSRPAQNAIEAVQWVYMGYLAAVKEQDGAAMSLGNVSSFLDIYIEQDIA
                     EGKISEEFAQELIDQFVMKLRMVRHLRMDAYNEIFAGDPTWVTESIGGALADGRHKVT
                     KTSYRFLQTLYNLGPSPEPNMTLLWSEKLPENFKKFCAQVSIDTSSIQYENDDLMQST
                     RCSDDYGIACCVSYQEIGKQIQFFGARTNLAKTLLLAINEGRCELSGQQVLTGIPKLK
                     SEVLDYEEVMEVFKLTMKEVARVYSETMNIIHYMHDKYYYEKAQMALIDTNPKINLAY
                     GVAGLSIVADSLSAIKHAKVTPIRDEHGVAKSFKIEGEFPFYGNDDDRVDAIAVEVPA
                     YFNQLLKKLPTYKNAEPTLSILTITSNVVYGKKTGATPDGRAHGVPFAPGANPMHGRD
                     HSGAIASLSSVSKLDYSEALDGISNTFSIVPKSLGPTDEERADNLVSIIDGYFNKEAH
                     HLNVNVLNREMLEDAMEHPENYPQLTIRVSGYAVNFVRLTRAQQQEVISRSFFESL"
     gene            9079..10344
                     /gene="ilvA"
                     /locus_tag="PLJ82_03900"
     CDS             9079..10344
                     /gene="ilvA"
                     /locus_tag="PLJ82_03900"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOR40681.1"
                     /translation="MNESRNSYFPQIADISKAYQRVKNVVKRTPLEYNANLSEEYQAQ
                     IYLKREDLQIVRSYKIRGAYNKMSCLSQEELERGVVCASAGNHAQGLAYACNKLRIKG
                     TIFMPAPTPKQKVRQVRMFGKEYVEIVLTGDTFDDAQFEADVFCRANNMVYIPPFNSK
                     KIIEGQGTIAIEILEDADQEIDFMFIPVGGGGLSSGVGAYFKQVSPHTQIIGVEPLGA
                     PAMYESLRTDNVVTLGTIDKFVDGAAVKKVGHLNFPICKEVLKEVALVPEGKVCSCIL
                     KLYNEEAIVVEPSGVLSIAVLDQYREQIKGKTVVCILSGSNNDITRMEEIKERSLLYE
                     GLKHYFIINFPQRAGALREFLNDVLSPTDDITTFEYTKKINREDGPALVGIEIQQKED
                     YNGLIDRMNKKGIRYQLLKDNPDVYRLLI"
     gene            complement(10394..10885)
                     /locus_tag="PLJ82_03905"
     CDS             complement(10394..10885)
                     /locus_tag="PLJ82_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HOR40682.1"
                     /translation="MSVNENPIITGFVEDSQLAGAGQVVYTKGTSPYEFFLQDQNKYS
                     YTGYFDGIEIGNHTVQVVDANMCKATFSFYLDIPIIPDLFFTPNGDGVNDVWTIKNIH
                     LVSHSVYIYDRFGKLLYTYEDDFDGWDGTYQGNPLPNTDYWFLNKIKPINKQLTGHFT
                     LKR"
     gene            11256..13358
                     /locus_tag="PLJ82_03910"
     CDS             11256..13358
                     /locus_tag="PLJ82_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR40683.1"
                     /translation="MKTINKLKMTILVVLSVMNLTWLQAQAPSQSDAVMLQGFYWDSQ
                     SQTNWTKLTTMASEIATNFDMVWLPPSSLSEGGVGYHPRQWNNQNSTWGSATELKTLI
                     STLKAGGCRAIADIVVNHRGGKTTWNDFYEDDFGSYGKFQLTSAHICKDDECVSKGYA
                     ATGANDTGYAVECGARGGYCAARDLDHANPYVQNDIKAYLKWLKGEFGYDGWRYDLTK
                     GYNGGYTNMYNLAAGAYYSVGEYWARDFNACWNWVKDANYNSTAFDFPMKYEALNDGL
                     AAGNYGKMIWSGGPAGLIHKPAYSRYSTTFVDNHDTYRDGNKYTGDVQKAYAFILSSP
                     GIPCVFWPHWTTNKSAINNMIKVRKACGIHNESVCSANANNGYYSETQGTRAKLICKI
                     GSTGTPSGYKVAASGTGWAFYVPSDFDVDANVPPKTAVSLTMNPEAGVYSGGTTVTLS
                     ATGNNTPISIYYTTNGTTPTSSSTKYTSPLSISSTTTVKAIAIDNNGNQTSVISKTYL
                     TESSTITLKFKAPSSWTQCNLWAWDPASGAKIAGGSSWPGNATMTLGSDGYYSYTISN
                     LTATTIGLLFNNGASTGTLQTVDLTTSSNACWDALTTTDTEGKYNVSLNTACTPTAIA
                     ENTASTVRCYPNPASTEMYIASEDAIKNVSIQDINGKQIWSDKSSVVDVSYFPSGLYV
                     IQVDTENGDRFIEKMIKK"
     gene            complement(13543..>14360)
                     /locus_tag="PLJ82_03915"
     CDS             complement(13543..>14360)
                     /locus_tag="PLJ82_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40684.1"
                     /translation="FQAVSAEESNLFEVFKIRVLPLPKVSISTNVHSCTEQDITIPFV
                     VEEGNPTHYSLFFDNPIFQNEQDKALVDNEITFEQPNGVLAGGYQVSIVFTQDESGCS
                     RSQIVPFFIDTSLVYAKWDDILFCDNVDLKFDTFQWFHNQVPISGATQQFLYSPSGFS
                     GEYHVEAYTSMGVKYTSCTTHYEAIPKSRTAMRVYPTPASRNHMVTIELPIEIDNVDL
                     GKLVIKNVVGQIVKQATVLQQKTSIILPSLPGMYMLEYIKEGKIMDVEKIQIY"
BASE COUNT         4673 a         2794 c         2520 g         4373 t
ORIGIN      
        1 tgtattagta gatggcgttt cgtatgaaat ggtaccaccc gttacagtag aatcgcaata
       61 tgctcccgct cccaatgccg tagcactcat taccgccatg gcggccaaca acaaaaatct
      121 ctttatgtag tttgtaattg ttttcataat tattctcctt tctggttatt aattacacga
      181 aaagccatat ttttacggta tctgataatg ccatataaaa ctattaataa aagcataaat
      241 ggtatcgcat cgcctatagg caatgagtat gtcctatcac aagcaagatt acactcaatc
      301 atagcagcat gacacgctgc ataatttcca ccatataaac aagtgatcat ttcgtaagaa
      361 cagcagtcat agcatatctc gcttttcgga tattttgtag gacagctgga agctccaagt
      421 gccgtataag aatatgaagg aaccgattga ataggatcaa ttgatatcgg gtcataacca
      481 ccatcaccag agcctcccga tgggacaaat gaacttgatg gcagtgtaac cgccccaaca
      541 gtcatactgc ttattataaa gaacaaaagg cttattaatt taatatgttt tttcattttc
      601 tgaatttgtg ttcgttatca ttctccattt atatgctatt gtgagaatcc ggcgcaaaat
      661 tacgcaagat tcttcacata gccaaataga ttcaatatca cttaataatc actttttgtt
      721 cttttgtcat cgtgtctccg actacagaaa ttagatatac acctttcgca acaggtaaat
      781 ctaatttttg ataatggctt cccgataatt tgtatgtatc tgtttttcct gccatatcac
      841 ttacataaac cgtttgaaga gctacacctt cggtagatga aattataact gaaccattct
      901 gtgcaaaaat actaattgac tctcctacaa gctctgtatc ttcaacatcc gtaaataagg
      961 gtgaatatga ttgcgtataa gcctttttaa tctgtgtaga ttgaaatgtt tgcggagttt
     1021 gcactagacg aaatgcagca cgactaacgg atttattctc ctgattgtcg gatagttgac
     1081 gaatagaatc agttagttca acaacaggaa tagcattccc cttttcagcc gccataccaa
     1141 atgtacgttc aatagtagat gtactaggaa aaggtcgtac gccatcatgt atagctacca
     1201 ataccccttc gggcactagc gttaacccgt tggcaacgga ataaaaacgc tcattccctc
     1261 cattaacaat tgtatgtgtg agttcaaatg catgttgttg gcataatttt ttccacatat
     1321 ctatatcgtc ttcgggcaat acaagaataa tctgtatgga atgatcgtat aaaaaaaatg
     1381 tttctaaggt atgcatcaaa attggtttac catgcaacaa taaaaattgt tttggcatct
     1441 cactttgcat acgttttcct ttaccgccag ctactacaac gacatatttt gcttgtatgt
     1501 tcgacataaa aaattgggtg ttataatttg tacaaaagta ctatttttct acaaaaatac
     1561 ctgtaaaagc gatttttaga ataaattttg gtatttttgc acataaaata acgaaaaaag
     1621 tatggcaaaa caggtattat tcagtattct ttttggtttt ttaatggcaa ttcaaggaat
     1681 ggctcaagaa aatcctttgg aaaatgacag tttattgcaa gaagaaacaa tactatcgga
     1741 gcatgatcgt atggatacca ttttgtatag agccaaaaac acagcaactt ctgagattga
     1801 atttgtaagt attggtggat ttcgcgtaca agtgtatgcg ggcaaccatc ctcaaaaagc
     1861 acgagaagat gctatgctta tagacaaaac tatcaaggaa caatatcctg aataccatgc
     1921 ttatgtatct tttcaagccc ccttttggcg agtacgtata ggagactttg catcgtacta
     1981 cgaagcgtta ttattaagca gagaattaaa aaagatattc cctgattttg ggaacgaaat
     2041 ttatgtagtt aaagacccca ctgttcgtat tttatatttt taacgtatgg aaaaagaaaa
     2101 accagctagt tctccagaaa tattgcaaga attggcatcc cattataaag ctgtaattga
     2161 acttattgga gaaaacccta caagagaagg tttgctaaaa acgcccgaac gcatggcaaa
     2221 aagtatgcag tttttgatgc gtggttacaa ccaagaccca gaaacaatta ttcgttcggc
     2281 aatgtttacc gaagattacc gccaaatggt aattgtaaaa gacattgatt tttattcgat
     2341 gtgcgaacac cacatgttac cgttttttgg acaagcacat gtagcttata tccccaacaa
     2401 gaaaattaca ggattgagta aaatcgcccg cgtagtagat gtatttgctc gtcgtatgca
     2461 agtacaagaa cgattaacta cacaaatcaa agagtgtatt caaaacactt taaatccgct
     2521 tggcgtaatg gttgtaattg aggctcaaca tatgtgtatg caaatgcgtg gagtggaaaa
     2581 gcaacattcg gttacaacta cttctgattt tacgggaatt tttgaacaag ctaaaactag
     2641 agaagaattt attagtttga taagcaaaaa ataattgatt tgagcatcgt aataaaaaaa
     2701 taaagcagac caaaaatctg ctttattttt ttattccact ttttttcgta attcaaaact
     2761 ggcacccaaa tatttttctc gcactacagg attatctgct agctcctcag ctgttccctg
     2821 aaaaagaatt tttccttcaa acaacaaata tgctctatct gtaatactca aggtttcgtg
     2881 cacattatgg tcggtaatta aaatgccaat gtttttttct ctcaactgtg ctactatacg
     2941 ctgaatatcc tccacagcaa taggatcgac acctgcaaat ggctcatcaa gcataataaa
     3001 tttaggttca atggctaaac aacgtgctat ttcgcaccta cgacgctcac cgccagacag
     3061 ttggtctccc aaacttttgc gtactgtttg caagctaaac tcactgatta atgtctccaa
     3121 cttctcgtgt tggtattttt tagagaattt tgtcatttgt aataccgaca aaatattgtc
     3181 ttccacactc atctttctaa acaccgaatt ttcttgagcc aaatatccaa ttccttgctg
     3241 agctcgctta taaaccggat acgaagatat atcgagatcg tttagaaaaa tacgtccttg
     3301 attaggtgtt accaagccta ctgtcatata aaacgtagtt gttttaccgg ctccattcgg
     3361 accaagcaag ccaacaattt caccctgttt cactgagatg gaaacattgt caacaactgt
     3421 acgtgttttg taccttttta ctaaatgctc cgtgcggagt accatttgat tttctgataa
     3481 catagattaa atatatccct cgccgtattt aagatttaca tccgttacaa aatgacgtat
     3541 gcgttgttcg tcttcttttt tacaaatcaa caacacattg tcagactcta ccacaatata
     3601 accagaaagg ttttgaataa ccgctaattt attttcgggc aataccacaa tattgtcgtt
     3661 actttcgtaa aattgagttt tgcatttaag tgttacattt tgatgctcat ctttttctga
     3721 catatcgtac aatgaacccc atgtcccaag atcagaccac ccaaaatcac cacacaatac
     3781 atacacattc ttggcatttt ccataatacc atagtcaata gaaatgtttt gacacgatgg
     3841 gaaaatttcg ttgataaaat cttgttctaa gtctgtatta aatttatcgc tacctgcttc
     3901 gaacttggct accatttcgg gtaagaactc gttgaatgct ttccaaattg cttccacact
     3961 ccatacaaat acacctgagt tccaaaagaa ttcgccactt tcgacaaaaa cttttgctaa
     4021 ttcaaggttt ggtttttcag taaacgtttt cactttttta aaatcgccca tagcctgatc
     4081 gcttatttgg atatacccat agcccgtttc aggacgattt ggcttaatgc ccagagtaag
     4141 caaattagga tgctcattta caaaatttaa actacgctct attacgttca aaaaatctat
     4201 ctcttttata atcaagtggt cagctggagc cactaccatg ttggcttttg gattaattgc
     4261 tttgatgcga ctaactgcat aggctacaca aggggctgta tttctgcggt ttggctctaa
     4321 caacacttga gatggtttga tgagaggtaa ttgttctaga atgatatctt tgtaatcttt
     4381 gttgcttaca atgagtatat ttgatgctgg tactactttt gcaaaacggt caaaagtcat
     4441 ctgaagcaag gtacgacctg taccaaaaaa atccaaaaat tgttttggca ttgaattttt
     4501 actgaagggc caaaagcgac ttcctacccc acctgccata ataacacaat agttgttgta
     4561 attcatacga ttgatagttt aattggtgtt gtgaaattac atttttgtaa aaaaatagaa
     4621 aacatcaggg ttatttattt tagatatctc actttataaa acccatctac ttaatttgaa
     4681 ggcaaagata cggaaagcaa ggtaataaaa aaatattttc agaaaaaaga agtttctgta
     4741 ttgctcaaat aaaaatttta tgtatttttg cactctccaa ttgcccagat ggcggaattg
     4801 gtagacgcgc tggtctcaaa caccagtgga ttcacttcca tgccggttcg atcccggctc
     4861 tgggtacgaa aagacctctt aattgattga ttaagaggtc tttttttata ctatttttcc
     4921 tctttttcgt acacatacca atacgccatg cctacaagca agccgccacc aacgatattg
     4981 ccaagggtag caggaataag gttatcgaag ataaaggatg cccaagatac atcagctcca
     5041 tacaagatag ctgtagggat aaaaaacata ttggcaatgc tgtgttcaaa accaagcgtt
     5101 acaaaagcca ttacaggaaa ccaaatagcc actatttttc caactacatc tttggaggca
     5161 aaagacaacc acatggctaa tgctaccaac caattagctc caataccttt gagaaaggtt
     5221 ttataaaatg ggttcgctgt ttttacctcg gcaatatgca taatgcctgc attccatgga
     5281 tcattatcga ataagccagt aaggtatgtc atggcatacg ctacaaaaag agccccaata
     5341 aagttgccaa catacaccag tgtccaattg cgtagcaaat cagtaatttg caatttaccc
     5401 gacataacag gcggaataaa ataggctgta tttcctgtaa acaaatcagc acctgcaata
     5461 gcacacatta tcagtccgat agggaatacc gcacccatta agaactttgg aatacccgga
     5521 ttatgttcgg ctatacccgg tacacctgca ccaatcatta cagcaagcaa accgccaatg
     5581 gcaatatatg cacctgctaa aaaagccaat actaaggttt ttgatactgt tgtttttgct
     5641 cgcatctcag cagcatcgct cgccacagac actaattgct tgggaagtaa ataattcata
     5701 gttctatttc ctattcgtat ttgttgtgtt agtttactac tacttctttt ccgtatgtgg
     5761 ataatatttt tttagccttg gttaattgtt cggctgtatt catctctaca tcttcgagct
     5821 ggtattgcca cccaagtgct tcgtatttgt gtttaccaag tttgtgatag ggctgtatct
     5881 cgattttttc tacgtttttc atgtctttta caaatgctcc aagttgatgc aaatgttctt
     5941 gttggtcggt ataaccaggc acaagtacat agcgaatcca tgtaggcttg ccaatttctg
     6001 ccaaataagt cgcaaaacgc aaagtaagat gatttttaac cccagtaatt gcctgatgag
     6061 cttcttcatc catctgtttg atatcgagca ataccaaatc ggtataggtc aataattctt
     6121 ttaccgcatc agtcaacaca ctcccattgg tatcgatgca tgtgttaaaa ccatcttctt
     6181 tgagggcttt aaatatcggt atcaatgcac gagcttgtag caaaggttca cctcccgaca
     6241 ccgtaacccc gcctttcttt ccgaagaaag gacgctgatt acgagccatt ttgagtatat
     6301 cgtctgcacc ataaggtcgt cctgttttag gctcgatagt gtctggatta gcacaataga
     6361 ggcacttgaa attacatcct tgtaaaaaaa gcaccaagcg aataccaggt ccatcgtacg
     6421 tacctaacga ctcaaccgaa tgtacattaa tcatgtagtg tatttataaa attagagtga
     6481 ttcgaagaaa cttctcgaaa ttacttcttg ttgttgtgct cgtgttaaac gaacaaaatt
     6541 tacagcatat cccgaaacac gaatggttag ctgtgggtaa ttttctggat gctccatagc
     6601 atcttccagc atctcacgat tcaacacatt cacatttaag tgatgagctt ctttgttaaa
     6661 ataaccatca ataattgaca ctaagttatc tgcacgttct tcgtctgttg gtcctaatga
     6721 ttttggaaca atggagaatg tgtttgatat accatcaaga gcctcagaat aatctaattt
     6781 agataccgaa ctgagtgagg caatcgctcc actatggtcg cgtccgtgca taggattagc
     6841 tccaggagca aaaggcaccc catgagcacg tccatcaggc gtagctcctg tttttttacc
     6901 atacactaca ttggatgtaa tggtaagaat ggacaaggta ggttcagcat ttttatacgt
     6961 tggcaatttt ttcaataatt gattgaaata tgcaggtact tctacagcaa tggcatctac
     7021 acggtcgtca tcattgccat aaaaaggaaa ttctccttca attttaaaac tttttgccac
     7081 tccatgttca tcacgtatag gggttacttt agcatgtttg atggcagata atgaatcggc
     7141 tacaatactt aaaccagcta caccataggc taaattaatt tttggattgg tatcaatcaa
     7201 agccatttgt gctttttcgt aataatattt atcgtgcatg tagtggataa tgttcattgt
     7261 ttcgctatac acacgtgcca cttctttcat ggtaagttta aacacttcca ttacctcctc
     7321 ataatcaagc acttctgatt tcaactttgg aatacctgtt agcacttgtt gaccagaaag
     7381 ttcgcaacga ccttcgttga tagccaacaa taatgttttt gctaaattgg tacgagcacc
     7441 aaagaattga atttgtttgc caatttcttg atacgataca caacaagcaa taccataatc
     7501 atcactgcaa cgtgtacttt gcatcaaatc atcattttcg tactgtatag aagatgtgtc
     7561 aatagatact tgagcacaga attttttaaa gttttcgggt aatttttctg accataacaa
     7621 ggtcatatta ggctcgggtg atggtccgag gttatataat gtttgtaaga aacggtatga
     7681 tgttttggtt accttgtggc gtccgtcagc caatgccccc ccaatagact cagttaccca
     7741 agttgggtcg ccagcaaaaa tttcgttata cgcatccata cgcaaatgac gtaccatacg
     7801 caatttcatc acaaactggt cgataagctc ttgagcaaac tcttctgaaa tttttccttc
     7861 agcaatatct tgttcaatgt aaatatctaa aaacgacgac acattaccca acgacatagc
     7921 tgcaccgtct tgttctttta cggcagctaa ataacccata tatacccatt gtacagcttc
     7981 tatcgcattt tgtgctggac gagacaaatc aaatccatag gatttaccca tttcgataat
     8041 ggcatacagg gctttgattt gctcagaaac ctcttcacgc aaacgaattc tttcatcgct
     8101 cataggtcct agcaagcttt ctaaatcttt tttcttttgt tgaataagaa aattaacacc
     8161 atataatgcc acacgacgat aatcgcctat tatacgacca cgagcataat tatcgggcaa
     8221 acccgtaagc acacctaacg aacgaaatgt tctaatttca ggtgtataca catcaaatac
     8281 gccatcattg tgcgtttttg catatttagt aaatacttct tttacaaatg gactaacctc
     8341 aacaccattt tcgctacatg ctttttctac tacggcaata cctccatagg gtttcatagc
     8401 tctgcgaagt agttggtctg tttgaagccc tacaattaac tctttttcct tatcaatata
     8461 ccctggagca tgcgaagtaa ttgtagacac taattcattg tcaatggagc gaacgccatt
     8521 attggcacgc tcttctgaaa tggcatcttt acatatttgc cacaaatcga gtgtacgttg
     8581 tgtaggtgaa cacaaaaagc cactatctcc atcgtatgga gtaatattta aggaaacaaa
     8641 atctcttaca tcaaccgcat tgttccattt tccgttttta aaattcatag tgtaaagtat
     8701 ttagagtatt atatataatt atgctggtaa tttgatgcca caaaactact gtatttatgt
     8761 tgtacaacat attattttag caaaaagaca tttctattta gtgattttta agttaaatat
     8821 taccattgta ttacaaaata ccgattcaca aaataaaatg agtctatatc gctgcatagc
     8881 cgataaacag agactttcta ccgaatcgtt atgtaattca gccaacaact aataacctgc
     8941 ctattttaca caatcaaatc ttacaaacta tctacaaaaa gatgaatttt gtaatgattt
     9001 acattgttaa tagtgcgttt ttggttacat aacacaaaat ccctatattt gcatccaaaa
     9061 ttcaaacgct acagaagtat gaatgaatca agaaattctt attttccaca aatagctgac
     9121 atttcgaaag cttatcaacg agtaaaaaat gtggtaaaac gtacaccttt ggagtacaat
     9181 gccaatttgt cggaagaata ccaagctcaa atttatctga aacgggagga tttacaaatt
     9241 gtacgttcct acaaaattcg tggtgcctac aataaaatgt cgtgcttatc gcaggaggaa
     9301 ttagaaagag gcgtggtatg tgcaagtgcg gggaaccatg ctcaaggctt ggcatatgct
     9361 tgcaataaat tacgcattaa aggaaccatc tttatgcctg cacccacacc caaacaaaaa
     9421 gtgcgtcaag tacgtatgtt tggaaaagaa tatgtagaaa tagttctaac tggcgataca
     9481 tttgatgatg cacaatttga agcagatgta ttttgcagag ccaataatat ggtatatatt
     9541 ccgcctttca acagcaaaaa aattatagaa ggacaaggca ccattgctat tgaaatacta
     9601 gaagatgctg atcaagaaat tgattttatg tttattccgg ttggaggagg aggtttatca
     9661 tcgggggttg gagcctattt taaacaagtg agcccacaca ctcaaatcat tggcgtagag
     9721 cctttggggg ctcctgctat gtacgaatcg ctacgcacag ataatgtggt aacacttggc
     9781 acaattgata aatttgtgga tggagcggcg gttaagaaag ttggtcacct gaattttcct
     9841 atttgcaaag aagtcctcaa agaggttgct ctggttcccg aagggaaagt gtgttcgtgt
     9901 attttaaaac tctacaacga agaagccatt gtagtagagc catcgggcgt attatcgata
     9961 gccgtactcg accaataccg cgagcaaata aaaggaaaaa cagtcgtatg tattctatca
    10021 gggagcaata acgacattac ccgaatggaa gaaattaaag aacgttcact cttatacgaa
    10081 ggtctgaaac attatttcat tatcaatttt ccgcaacgtg caggagcctt acgtgagttt
    10141 ttgaacgatg tgttaagccc tacagatgat atcaccacat ttgaatacac aaaaaaaata
    10201 aacagagaag atggacctgc attggtaggt atagagatac aacaaaaaga agattataat
    10261 ggacttatcg acaggatgaa taaaaaagga atccgttatc aacttcttaa agacaatcct
    10321 gatgtatatc ggttgttgat ataattatca gaaactatgt tggcaatagt ccctgataat
    10381 acttagaacc caactacctt tttaaagtaa aatgacctgt cagctgttta ttgataggct
    10441 ttattttatt caaaaaccaa taatcggtat ttggtaatgg atttccttga taagtaccat
    10501 cccatccatc aaaatcatct tcgtaagtat acagtaattt accaaaacga tcataaatat
    10561 aaaccgaatg agatacaagg tgaatgtttt ttattgtcca cacatcattt acaccatcgc
    10621 cattgggagt aaaaaataaa tctgggataa tgggaatatc aagataaaaa ctaaaagtag
    10681 ccttacacat gtttgcatct actacttgca cagtatgatt acctatttct attccgtcaa
    10741 aatagccagt atatgaatat ttattctgat cttgcaagaa aaattcatac ggagaagttc
    10801 cttttgtgta tacgacctga ccagcccctg ccaattggct atcttctaca aaaccggtaa
    10861 taattggatt ctcattaacg gacaaagtga ttaaatcagc agcagatata ccccctgtaa
    10921 caaaaaaaaa gaattatcaa aagatgtact cgttggattt agagtacagt agccttcata
    10981 taaataaaga atatttaagt taaataacat ataaaaaatt aagaacaagc atagtaagaa
    11041 aaacccaacc ttgatgaaaa atatataaca atatttaata tttactccac gtaacctatc
    11101 caactcacat tataaataaa acaaatagtc atctgcttca tttataagca aaaacaatta
    11161 tctcaagatt taacactaat taacacccca tagggttggg ctgaaggtat aaccaattgt
    11221 tttagtagta aattacaaac cttaaactat tttttatgaa aacaatcaac aaacttaaaa
    11281 tgactatttt agtagttttg agtgtcatga atttaacttg gctgcaagct caagctccaa
    11341 gccaaagtga tgctgttatg ctacagggtt tctattggga ctctcaatcg caaacaaact
    11401 ggacaaaact caccacaatg gctagcgaaa tagctaccaa ttttgatatg gtttggttac
    11461 ccccatcttc tttatcagag ggtggcgtag gataccatcc aagacaatgg aataaccaaa
    11521 atagcacatg gggtagtgct actgaactaa aaacccttat tagcacctta aaagcgggag
    11581 ggtgtagagc tattgccgac attgtagtaa accatagagg cggtaaaacc acatggaatg
    11641 atttttatga ggatgatttt ggctcgtatg gtaaatttca acttacttct gctcatatct
    11701 gcaaagatga cgagtgtgta agcaaaggat acgctgctac tggagccaat gatactggat
    11761 acgctgtaga atgtggtgcg cgggggggat attgcgctgc tcgcgattta gaccatgcaa
    11821 atccatatgt acaaaatgac attaaagcat atctaaaatg gcttaaagga gaatttggct
    11881 acgatggatg gagatacgat ttaacgaaag gatacaatgg cggatatacc aatatgtata
    11941 atttagctgc tggagcttac tattctgtgg gtgagtattg ggctcgggac tttaatgctt
    12001 gttggaattg ggtaaaggat gctaactaca actcaacagc ttttgacttt ccgatgaaat
    12061 acgaggctct gaatgatgga ttggcagcag gtaattatgg taagatgatt tggtcaggtg
    12121 gtcctgcagg acttattcat aaaccagcgt atagtcgcta ttccactact tttgtagaca
    12181 atcacgacac ataccgtgat gggaataaat acacaggaga tgtacaaaaa gcatatgctt
    12241 ttatcttatc atcaccaggt attccttgcg tattttggcc tcattggaca actaacaagt
    12301 cagcgattaa caatatgatt aaagtacgta aggcatgtgg cattcacaac gaatcggtat
    12361 gtagtgcaaa tgcaaacaac ggatattata gtgaaacaca aggaacacga gcaaaattaa
    12421 tatgtaaaat cggttctaca ggcacaccaa gtggatataa agtggcagct tcgggaacag
    12481 gttgggcatt ttatgtacct tcagattttg atgtagatgc taatgttcct cccaaaacag
    12541 ctgtttctct tacaatgaat cccgaagcag gggtatactc agggggaacc actgtaaccc
    12601 tttctgctac aggcaataat acgcctattt ctatatatta tactacaaat ggaacaaccc
    12661 ctacttcatc tagcacaaag tacacatcac ctctatccat cagttcaaca acaactgtta
    12721 aagccatcgc aattgacaat aatggaaacc aaacatctgt aatatcaaag acatacctta
    12781 cagagtcctc cacaataact ctgaaattta aagctcctag ctcatggact caatgtaatt
    12841 tatgggcatg ggatccggca tccggcgcca aaattgctgg cggttcttct tggcccggaa
    12901 atgctacaat gacattgggg tctgatggct actattcata tacaatatct aatttgacgg
    12961 ctacaactat cggattattg tttaataatg gagcaagtac aggaactttg caaaccgtag
    13021 atttgacaac atcatccaat gcatgttggg atgcattaac aacaaccgac actgaaggga
    13081 aatataacgt aagtttaaat actgcatgta ctcccacagc tatagcagaa aatacagcat
    13141 ccacagttcg atgttaccca aatcctgcct ctacagaaat gtatattgca agcgaagatg
    13201 ccatcaaaaa tgtgagcatt caagatatta atggaaaaca aatatggtct gataaatcaa
    13261 gtgtggtaga tgtatcttat ttcccttcag gattatatgt tattcaagtt gataccgaga
    13321 atggagaccg atttattgaa aagatgataa agaaataagc atttaaaaat agaaaatttt
    13381 acggggttga tgggatgctt ccattgaccc cgtatttttt gaaaatcaaa ataaatattt
    13441 gattgataat atcttaggaa tatctataga gtgattattt tggggacatt gaagattata
    13501 aatatggtct atgcaaaaaa aagcaacaac agctcaataa tattaatata tttgaatctt
    13561 ttctacatcc ataattttgc cttcttttat atactctaac atatacatac caggaagaga
    13621 aggcagaatt atggatgttt tttgctgtaa aactgttgct tgttttacaa tctgaccaac
    13681 tacattttta ataactaact tacccaaatc tacattatct atttctatag ggagttcaat
    13741 agttaccata tggtttctac ttgctggagt aggataaaca cgcattgctg ttctactttt
    13801 cggaatagcc tcataatggg tagtgcaact ggtatacttt actcccattg aagtatatgc
    13861 ctcaacatga tattcgccag aaaaaccact tggtgaatac aaaaattgtt gcgtagcacc
    13921 cgatataggt acttgattat gaaaccactg aaacgtatca aactttaagt caacattatc
    13981 acaaaaaagg atatcatccc atttagcata aaccaaagaa gtatcaataa aaaaaggcac
    14041 aatttgtgag cgagaacaac ctgattcatc ttgtgtaaaa acaatagaca cttgatatcc
    14101 accagccaac actccattcg gttgctcaaa ggtaatttca ttatctacta atgctttatc
    14161 ttgttcattt tgaaatattg gattatcaaa aaacaaacta taatgggttg ggtttccctc
    14221 ttccacaaca aaaggaatag ttatgtcttg ttctgtacaa ctatgaacat tggtcgatat
    14281 acttaccttg ggtaaaggaa gcacccttat cttaaatacc tcaaataagt tactttcttc
    14341 agccgaaaca gcttgaaata
//
LOCUS       DAOSRP010000042        14163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4371_length_14163_cov_4.675432, whole genome shotgun sequence.
ACCESSION   DAOSRP010000042 DAOSRP010000000
VERSION     DAOSRP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4371_length_14163_cov_4.675432"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="recA"
                     /locus_tag="PLJ82_03920"
     CDS             complement(<1..838)
                     /gene="recA"
                     /locus_tag="PLJ82_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOR40685.1"
                     /translation="MATKDTENQPKVNVEKLKALQLALDKIEKDHGKGTIMKMGDKTV
                     VEVNTISTGSIGLNVALGVGGFPRGRVIEIFGPESSGKTTLAIHAIAQAQKAGGIAAI
                     IDAEHAFDRFYAEKLGVDINNLLISQPDNGEQALEIADQLIRSSAVDIVVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTANINRTNTTCIFINQLRDKIGITFGNPET
                     TTGGNALKFYASIRLDIRRIGQIKDGDEVIGNQTRVKIVKNKVAPPFRKAEFDIMFGE
                     GIS"
     gene            complement(993..2039)
                     /gene="ilvC"
                     /locus_tag="PLJ82_03925"
     CDS             complement(993..2039)
                     /gene="ilvC"
                     /locus_tag="PLJ82_03925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOR40686.1"
                     /translation="MAQMNFGGVVENVVTREEFPLEKAREVLKNDTIAIIGYGVQGPG
                     QSLNLRDNGFNVIIGQRKGGKTWEKAIADGWVPGETLFDIEEACQKATIIQYLLSDAA
                     QIEVWPRIKPYLTAGKALYFSHGFGITYKERTGIVPPADVDVILVAPKGSGTSLRRMF
                     LQGRGLNSSYAIFQDATGKAFERVVALGIGVGSGYLFETTFKKEVYSDLTGERGTLMG
                     AIQGILLAQYEVLRENGHSPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRG
                     ALDWMTPFHDATKPVFKKLYEEVACGNEAQRSIDTNSKPDYREGLEKELKALRESEMW
                     QAGAVVRKLRPENK"
     gene            complement(2206..2763)
                     /gene="ilvN"
                     /locus_tag="PLJ82_03930"
     CDS             complement(2206..2763)
                     /gene="ilvN"
                     /locus_tag="PLJ82_03930"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOR40687.1"
                     /translation="MEKKLYTITVFSENMPGILNQITIVFTRRQLNIETLSVSPSALK
                     GIHKFTITVETTLDVVRKVVTQIDKRIDVIKAYYNTDDELVVQEIALYKVATDKVLEL
                     GGIEQLIRKHNIRILELTPTCIVFEKTGMYEETQHLFEELQNSIGVLQFIRSGRVAIT
                     KSKVERLSDMLASIEECIKQQKIVK"
     gene            complement(2767..4464)
                     /gene="ilvB"
                     /locus_tag="PLJ82_03935"
     CDS             complement(2767..4464)
                     /gene="ilvB"
                     /locus_tag="PLJ82_03935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOR40688.1"
                     /translation="MEKKTMQGSYALLESLLNEGVDTIFGYPGGAIIPVFDAMYDYYD
                     RLRYILTRHEQGATHAAQGYARVSGKVGVCIVTSGPAATNAVTGISDAMLDSTPLVVI
                     TGQVSAALLGTDAFQEIDVVSITQPITKWSYQIRKADEIAWAVARAFYIAQSGRPGPV
                     VLDIAKNAQLENVDFIPQKCTFIRSYIPTCKLDDTVAKQAAKLINEAKKPFALVGQGV
                     TLGNAEDELLAFIEKANIPAAWTLLGESAMPTDHPLNKGMLGMHGNIGPNLKTNECDV
                     LIAIGMRFDDRVTGNLNEYAKQAKVIHFDIDPAEINKNVQTEVAVLGDVKTTLPAVTQ
                     YLVKNSHTEWIASFKEHEQKEYDFVIQKETESTEGAIKMGEVVLKVSKATNHSAILVS
                     DVGQNQMMAHRYFRFTQKRSNVTSGGLGTMGFGIPAAIGAKIGAPNRTVCLFCGDGGF
                     QMTIQELGTIMQEKIGVKMIILNNHFLGMVRQWQELFHQERYSSTPMENPDFITIAKA
                     YNINGKEVSERHELDMAIKEMLADDKPYLLVVNVEKNNMVYPMIPAGAAVTNILLGE"
     gene            complement(4546..6375)
                     /gene="ilvD"
                     /locus_tag="PLJ82_03940"
     CDS             complement(4546..6375)
                     /gene="ilvD"
                     /locus_tag="PLJ82_03940"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOR40689.1"
                     /translation="MKQQLRSATSTQGRRMAGARALWRANGMQENQIGKPIIAVVNSF
                     TQFVPGHVHLHQIGQLVKQEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMVNAHKADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGELDNKHLD
                     LIDAMIQSADPSVSDEEVDRIERAACPTCGSCSGMFTANSMNCLNEAIGLALPGNGTI
                     VATHANRKQLFIEAAKMIVDNTYKYYANGDDSVLPRSIATRDAFLNAMVLDIAMGGST
                     NTILHTLAIAYEGNVSFNMDDIDALSRKTPCLCKVAPNSQKYHIQDVNRAGGILGILA
                     ELAKGNLINTSVKRVDGLTLQEAIDRYDITSQSASELAIKKYKSAPAHRFNLVMGSQE
                     TYYPALDTDRENGCIRSVDKAYTKDGGLAILKGNIAIDGCVIKTAGVDESIWKFSGTA
                     KVYQSQDDACYAILKGDVKAGDVVVITHEGPKGGPGMQEMLYPTSYIKAKHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGAIGLVKDGDIIDIDIPNRSINVRISDNELENR
                     RKEEEGKGKEAWKPIRDRKISTALKAYASMVTSADKGAVRVVE"
     gene            6706..8454
                     /locus_tag="PLJ82_03945"
     CDS             6706..8454
                     /locus_tag="PLJ82_03945"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOR40690.1"
                     /translation="MEDKLVLEVRAKAQRWLDGNYDEETKKQVKAMLDNNDTTDLIES
                     FYKDLEFGTGGLRGIMGVGTNRMNIYTMGAATQGLANYLIKEFGKNSSIKVVLGHDCR
                     NNSRLFAETAANIFSANGIKVYLFEDLRPTPEVSFAIRHLGCQSGVMLTASHNPKEYN
                     GYKAYWNDGAQVLAPHDTNIIREVNAIKSVDDIKFKGNPALIEIIGKEIDDIYLNKIN
                     TISLSPDSIKRNSDMKIVYTPIHGTTVRLVPAALKAYGFTNIINVPEQDVVSGDFPTV
                     YSPNPEEPAALKMAVEKGEEVDADLVMASDPDGDRLGIAVKDDTGKFVLVNGNQTALL
                     FTYYIIRRWKELGKLNGKQYVVKTIVTTELIVDIAKKFKLHYFDCYTGFKWIAAIIRE
                     NEGKLQYIGGGEESYGYLAQDFVRDKDAVSACTLMAEIAAWAKDNGKSLFELIQDIYL
                     EFGFSKEKGISIVKKGKSGAEEIKKIMSNLRSKPPKEIAGSQVIVIKDYDTLRLIDVS
                     AGTTNAMEMPETSNVLQFFTADGTKISVRPSGTEPKIKFYIEVRGELKNRKDYEKANQ
                     VADEKIKAVMASLKLD"
     gene            8595..9101
                     /locus_tag="PLJ82_03950"
     CDS             8595..9101
                     /locus_tag="PLJ82_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40691.1"
                     /translation="MKKTLLVIFITLFSCLYLFSQNEVITEEPPSLRPLLVDTTDCCV
                     DCAMEMQFQSIEKALANKENVHCLVLSFSSPKYTAIPKEVAQFQHLTCLDISFNRVAV
                     IPEEILQCQQLYCLDLSGNHYLQDLPKFLKQLPNLKVIRLLDMTLWSSAKKQQLKKDF
                     EGIYLEFE"
     gene            complement(9176..10450)
                     /locus_tag="PLJ82_03955"
     CDS             complement(9176..10450)
                     /locus_tag="PLJ82_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40692.1"
                     /translation="MKVIYVVIALLTSLLYACAPSTENTEKYSVNEPIFMDATEFRSS
                     IAITQDVHPITSYGKISFYKGYLFVSEPQKGIHIINNTDAKNPQVVGYIELLGNADIA
                     IKDDILYADSYIDLVWFDISTPATPLFVGRLENVFEQALPPLEDPSFGIDGSMVWDRP
                     SDQVIVGWTIATRTIKTSNSIFSGFPWWSSSDPTPIMAEKGSTGINGSMSRFSLHDDY
                     LYSVINNNMSIINLSGAAPVKAVDNIYIGWDVETIFYYKNNMFMGTPTGLLIYSVENP
                     TQPVFKSSIQHVFGCDPVVVENDIAYVTIHSGNACGQDDNELIAIDVKDVENPKQVVS
                     YTMTKPKGLGIDNGTLFICDDGLKIYKADNPQTIMANELAHYTNIDGYDVIPFHNTLM
                     MIASDGIYQYDYSDLNNIHQISFLPMSNEKEE"
     gene            complement(10478..11704)
                     /locus_tag="PLJ82_03960"
     CDS             complement(10478..11704)
                     /locus_tag="PLJ82_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOR40693.1"
                     /translation="MKVITLFALGTIMLLNACTSTEPTENALKTITLEEGMEARVDEE
                     NSFAFDFLQETLSTTEEDNVFISPLSVSIAMGMTWNGANGATKEEIKQALKHQGLTDE
                     QINRYYQILLNNLPNLDKKTKLILANSIWYKKDFPILKDFLDKNNVYFNAKVQGLDFS
                     KSESLDIINNWCAEKTNNLIKKPLDQISDNSIAFLINAIYFKGLWKNKFDSKHTQEAT
                     FTAEDNTSTTVDMMNQTESFDYTEDDDAQYIDLSYGNGAFSMLVILPKQGKKINDIAP
                     NLTKTKWKSIISSLNTESIALQLPRFKVQNKMQLNPILVKLGIEKAFLPEEADFSGIS
                     SDAQLYISRVIHQTYCEVNEEGTEAAAVTIVDVAVTSIPIFKEFRANKPFLFLIREKS
                     TGIIVFAGKIGKIANE"
     gene            complement(11792..12523)
                     /locus_tag="PLJ82_03965"
     CDS             complement(11792..12523)
                     /locus_tag="PLJ82_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOR40694.1"
                     /translation="MENAKRLNKAISETGFCSRREADKLIAENRVLVNGTIGNLGMQV
                     NENDEIKVDGKRISKEVECIYIAFNKPPGITCTTDTSIKDNIIDFINHPLRIFPIGRL
                     DKASEGLIFLTNDGDIVNKILRSKNNHEKEYIVTVNKPITSDFLLKMSAGVPILDTIT
                     NPCKIKKINEYTFSIILTQGLNRQIRRMCEYLDYGVSGLKRIRIINISLGSLKKGQFR
                     YFTKRELNELLALVETSSKTHDTFL"
     gene            complement(12533..13753)
                     /gene="serB"
                     /locus_tag="PLJ82_03970"
     CDS             complement(12533..13753)
                     /gene="serB"
                     /locus_tag="PLJ82_03970"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOR40695.1"
                     /translation="MGNSEIILINISGDDQPGVTSAVAGILGKYNATILDIGQADIHH
                     TLSLGILFKTSNSFDAANILKEVLFKCSELNLNVRFTPISIPQYNEWVNRQGSGRYIV
                     TLLGKLITAEQLSIVTKAISEQELNIDSIKRLTGRPSLENLNDNVRSCIELSVRGDLK
                     NKDQLSSQFMSYASELGIDISFQKDDMYRRNRRLICFDMDSTLIQTEVIDELAEKAGV
                     GKKVRAITEAAMRGEIDFNESFIKRVALLKGLDESVMEEIAQNLPIMDGAKRLMTILK
                     KYGFKIAILSGGFTYFGNALKKQFDVDYVYANELEIVNGKLTGKHLGEIVDGKRKAEL
                     LRLIAQFEKIELEQVIAVGDGANDLPMLNLAGLGIAFHAKPKVKENAQQAISTIGLDG
                     ILYFLGFRDIHMNE"
     gene            complement(13857..>14163)
                     /locus_tag="PLJ82_03975"
     CDS             complement(13857..>14163)
                     /locus_tag="PLJ82_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOR40696.1"
                     /translation="KNLWIFSWQFYGNLIQHYSILPTPIKKFIYHFMITNRLYQYKSI
                     KGLHSSLQIMSTYKIKAINADKSIHVLIENKDILHRQFQLFFTELLQYTSEERSKTH"
BASE COUNT         4355 a         2486 c         2408 g         4914 t
ORIGIN      
        1 tagaaattcc ttcaccaaac atgatatcaa attcagcttt acggaaagga ggagctacct
       61 tattttttac aatttttacg cgtgtttgat tgccaatcac ttcgtctcca tctttaattt
      121 gtcctatacg acgaatgtct aaacgaatag aagcgtagaa tttcagtgca tttccccccg
      181 tagttgtttc aggattgcca aatgtaatgc caattttatc gcgtaattgg ttaataaata
      241 tacacgttgt atttgttctg ttaatattgg ctgtaagttt acgtaatgct tgcgacatta
      301 gtcgtgcttg taatcccatt tttgaatcac ccatatcgcc ttcaatttca gcttttggtg
      361 ttaaggcggc aacagagtct ataactacta tatctactgc tgaggagcga atgagttggt
      421 cggcaatttc gagagcttgt tctccgttat ctggttgaga aataagtaga ttattgatat
      481 caacccctaa tttttcggca tagaaacgat cgaatgcatg ttcagcgtca ataatagcag
      541 cgattccacc agctttttgt gcctgagcta tagcatgaat ggctagggtt gttttaccag
      601 acgattcagg accaaaaatt tctattactc ttccacgagg aaaaccacca acgcctaaag
      661 caacgtttag tccaatagaa cctgtagaaa tcgtgtttac ttctactacc gttttatcac
      721 ccattttcat aatggtgcct tttccgtggt ctttttcaat cttatcaagc gctaattgaa
      781 gtgcttttaa tttttctaca ttcacttttg gttgattttc agtgtctttt gtcgccatag
      841 ttgtatcagt ttatttgaac atcaaaagta taattttttt tgtcaatagc caattcattt
      901 tgttagtttg ttgaaaagtt tatgaacaaa aaaaagcgga caaatatctt ttggatagtg
      961 tccgctttta aaaagtgcaa ttaaatgcgt tattatttgt tttctggacg caatttacgt
     1021 actacagcac ctgcttgcca catttcgctt tcgcgaagtg ctttaagttc tttttctaaa
     1081 ccttcgcggt aatcaggttt agagttggtg tcgatagagc gttgagcttc gttaccacaa
     1141 gcaacttctt cgtataattt tttgaataca ggtttagtag catcgtggaa aggagtcatc
     1201 caatctaagg caccacgttg agctgtagtt gagcagttag catacatcca gtccattccg
     1261 ttttctgcaa acaatggcat taatgattga gttaattctt ctactgtttc gttaaacgct
     1321 tcagaaggag aatgtccgtt ttcgcgtaac acttcatatt gagctaacaa aataccttgg
     1381 atggcaccca ttaatgtacc acgttcgcca gttaaatcag aatatacttc ttttttgaaa
     1441 gtagtttcaa acaagtaacc agaacctaca ccaataccta aagctacaac acgttcaaaa
     1501 gctttgcctg tagcatcttg gaatattgcg taagatgaat ttaatccacg tccttgaagg
     1561 aacatacgac gaagtgatgt cccagaacct ttaggtgcaa ctaagataac atctacatca
     1621 gctggaggca caatgccagt acgttctttg taagtaatac caaaaccatg agagaaatac
     1681 aaggcttttc ctgcagttag gtatggtttg atgcgaggcc acacttcaat ttgagctgca
     1741 tctgacaata aatattgaat aatggttgct ttttgacaag cttcttcgat gtcaaacaat
     1801 gtttcgccag gaacccagcc gtcagcaatt gctttttccc atgtttttcc acctttacgt
     1861 tgtccaatga ttacattgaa tccattatca cgtaagttaa gcgattgacc aggaccttga
     1921 acaccataac cgataattgc aatggtatca ttttttagca cttcgcgtgc tttttctaaa
     1981 ggaaactctt cgcgggtaac tacgttttct actacgccgc caaaattcat ttgtgccatt
     2041 ttactattat tttatttatt aaaaggtaat tagttctttt taatttggtt taaacatctc
     2101 tacaaattat tttgtagaaa agcacgacaa aattactttt tttttattgt tttggcaaat
     2161 atttatgcta atttgtcgtg caaagactat cttttacttt cgaatttact ttactatttt
     2221 ttgttgttta atgcattctt cgatagaagc caacatatca ctaagtcgtt cgaccttact
     2281 ttttgtaata gccactctac cagaacgaat aaattgtaat actccgatag aattttgtag
     2341 ctcttcaaac aaatgttgtg tttcttcgta cattcctgtt ttttcaaaca caatacaggt
     2401 tggggtgagt tctaagatgc gaatattgtg cttacgaata agctgttcga tacctcctaa
     2461 ttccaacacc ttgtcagtgg ctactttgta taatgcaatt tcttgtacaa ctaactcatc
     2521 atcagtgttg taataagctt taattacatc aatacgttta tcgatttgag ttactacttt
     2581 tcgaacaaca tctaaggtag tttcaacagt aatggtaaat ttgtgtattc ctttgagagc
     2641 agaaggagaa acagaaagag tttcaatatt cagctgtcta cgagtaaata caatggtaat
     2701 ttgatttaaa atacccggca tattttctga aaataccgtg attgtataaa gttttttttc
     2761 cataagttat tctcctaata aaatgtttgt aacggctgct cctgcaggaa tcattgggta
     2821 aaccatgttg tttttttcta catttactac caacaaatac ggtttatcgt ctgctaacat
     2881 ttctttgatt gccatatcta attcatgtcg ttctgacact tcttttccat taatattgta
     2941 tgctttggct atcgttataa agtctggatt ttccattggt gttgatgaat agcgttcttg
     3001 gtgaaaaagt tcttgccatt gtcgtaccat tcctaaaaag tgattgttta aaataatcat
     3061 ttttacgcca attttttctt gcataatagt tcctaactct tgtattgtca tttggaaacc
     3121 tccatcgcca cagaataaac atacggtacg attaggggca cctatttttg caccaattgc
     3181 tgcggggata ccgaagccca ttgttcctaa tcctcctgag gtaacattgc tccgtttttg
     3241 tgtaaaacgg aagtaacgat gtgccatcat ttggttttga cctacgtcac ttactaaaat
     3301 agcactatgg ttggtagctt tagatacttt taaaacaacc tcacccattt tgatagcacc
     3361 ctcggtagat tctgtttctt tttgaatgac aaaatcgtac tccttttgtt catgttcctt
     3421 aaaagatgct atccattctg tatgagaatt tttcacaaga tattgtgtta ctgcaggaag
     3481 tgttgttttt acatctccta aaacggctac ttctgtttgt acgtttttgt taatttcagc
     3541 aggatcaata tcaaagtgaa taactttagc ctgtttagca tattcattta aattccctgt
     3601 tacacggtcg tcaaaacgca taccgatagc aattaataca tcacattcgt tggttttaag
     3661 gtttggtccg atatttccgt gcattcccag catacctttg tttaaaggat ggtcggttgg
     3721 cattgctgat tctcccaata atgtccatgc cgcaggaatg tttgcttttt ctataaatgc
     3781 tagtaattca tcttcagcat ttccaagcgt aacaccttga ccaactaaag caaaaggttt
     3841 ttttgcttca ttaattaatt ttgcagcttg tttggctacc gtatcatcaa gtttacatgt
     3901 aggaatataa ctacgaataa atgtacattt ttgtggaata aaatctacgt tttccaattg
     3961 tgcattttta gcaatatcca atacaacagg tcctggtcgt ccactttgtg caatataaaa
     4021 tgctcgtgct actgcccatg ctatttcatc tgctttgcgt atttgataac tccatttagt
     4081 gatgggttgg gtaatactta ctacatctat ttcttgaaaa gcatctgttc ctaataaagc
     4141 ggcacttact tgtcctgtaa ttacaactaa aggggtacta tctagcatag catcactaat
     4201 gcctgttact gcattggtgg cagctggacc agatgttaca atacataccc caactttacc
     4261 cgatacgcgg gcatagcctt gcgctgcatg tgtggctcct tgttcgtggc gagttaagat
     4321 ataacgtagc ctatcgtaat aatcgtacat agcgtcaaat actggaataa tggcacctcc
     4381 agggtagcca aagatagtat caacaccttc gtttaaaagg gattccaata aggcgtatga
     4441 accttgcatt gttttttttt ccatattata tagtttatat aaaatcaata aaattgaaat
     4501 agtataattg taaacaaaaa gaataaaaga gtatcttagt aaagattact caacgactcg
     4561 tacagccccc ttatcagcag atgttaccat gcttgcatag gcttttagtg ctgtagaaat
     4621 ttttctgtca cggataggtt tccaagcttc tttaccttta ccttcttctt cttttcgtct
     4681 attttctagt tcattatctg aaatacgtac attgatactt ctatttggaa tatctatatc
     4741 aataatatcg ccgtctttta ctaaaccaat agcaccacca gctgcagctt caggcgaaat
     4801 atgtccaata gataaaccag aagttcctcc cgagaaacgt ccatctgtaa tcagcgcaca
     4861 ttctttgcct aagtgtttag ccttgatata ggaggtaggg tatagcattt cttgcattcc
     4921 tggacctcct ttgggtcctt cgtgtgtaat aactacaaca tcacctgctt ttacatctcc
     4981 ttttaaaata gcataacatg catcatcttg cgactgatat actttggctg tgcctgaaaa
     5041 tttccaaata ctttcatcta ctcctgctgt tttaattaca cagccgtcaa tagcaatgtt
     5101 tcctttcaga atagctagtc caccatcttt tgtataggct ttatctacac ttctgataca
     5161 tccattttca cgatcagtat ctaaagctgg atagtatgtt tcttgcgaac ccataaccaa
     5221 attaaaacgg tgagcaggtg cacttttata tttcttaatg gctaattcgc ttgcactttg
     5281 gcttgttata tcatacctgt caatagcttc ttgcaatgtt aaaccatcta ctcttttaac
     5341 gctcgtatta attaagttcc cttttgccaa ctctgccaaa atacctaaaa taccgccagc
     5401 acggttaaca tcttgaatgt ggtatttttg tgaattagga gcaactttac acaagcaggg
     5461 tgttttacga gataaggcat caatatcatc catattgaaa gaaacattcc cttcgtatgc
     5521 aatggctaaa gtatgtaaaa tagtgtttgt agatccaccc atagctatat ccagtaccat
     5581 tgcatttaaa aaagcatctc gtgtagctat agaacgtggt aaaacactat cgtctccatt
     5641 tgcgtaatat ttatacgtat tatcaacaat cattttagct gcctcaatga ataattgttt
     5701 tcgattagca tgtgtggcaa caatagttcc attacctggt aatgctaaac caatagcttc
     5761 gtttaaacaa ttcatagagt ttgcagtaaa cattcctgaa catgacccac aggtagggca
     5821 ggctgcccgt tcaattcgat caacttcctc gtcagataca cttggatctg ctgattgtat
     5881 catggcatca atcaaatcta aatgcttgtt gtcaagttct ccagcttcca ttggaccccc
     5941 agaaacaaaa attgtaggaa tatttaatcg cattgcagcc attaacatac ctggagtaat
     6001 tttgtcgcaa ttgcttatac ataccatcgc atctgctttg tgtgcattta ccatgtattc
     6061 aacgctatca gctattaaat cacgacttgg aagcgaatat aacataccat catgtcccat
     6121 agcaatacca tcatcaatgg ctatggtatt aaattcagct gcaaaacagc ctaatttttc
     6181 aatttcttgt tttaccaact gacctatttg gtgtaaatga acatgacctg gtacaaattg
     6241 agtaaacgaa ttgacaactg caataatggg tttcccaatt tgattttctt gcataccgtt
     6301 ggcacgccac aatgctctgg ctcctgccat tcttctgcct tgtgtgcttg tagcacttct
     6361 tagttgttgt ttcattacaa tgtttgttat ttacctaaaa tatataaaaa taaaaaaagc
     6421 cctaccacac aggtgagaag gcttatcaca tatagaaaaa tacacacata accatctcac
     6481 cttatggaac tgaccacgag gataataatg cttaaaatga cggttagacg tgttttcatt
     6541 gtttaaatca ataatttagt tgcaaagata ggtatttatt tttaaaataa aagtgatttg
     6601 ccttaaaaaa tgtacaatcc tgaaaaaatt acattttttt tatatttcaa gggctgattt
     6661 tgaaaaaaaa acgtattttt gcctcaattt tttaataaaa caataatgga agataaatta
     6721 gttttagaag taagagcgaa agctcaacga tggcttgatg gaaattatga cgaagagacc
     6781 aaaaaacaag taaaagctat gcttgataat aatgatacta ccgatttaat agaatcattt
     6841 tataaagact tagaatttgg cacaggtggt ttacgtggca ttatgggtgt tggcactaat
     6901 cgtatgaata tatatacaat gggtgcagca acacaaggtt tagctaatta tttgattaaa
     6961 gagtttggta aaaattcatc tattaaggtt gtgttagggc atgattgtcg gaataatagt
     7021 cgcttgtttg ccgaaacagc tgctaatatt ttttcagcaa atggaataaa agtgtatttg
     7081 ttcgaggatt tacgacctac accagaagta tcgtttgcta ttagacattt aggttgccaa
     7141 agtggtgtaa tgcttaccgc aagtcataat cctaaagaat ataatggata caaagcgtat
     7201 tggaatgatg gagcacaagt gttggcacca catgatacga acattattcg cgaggtaaat
     7261 gccattaaat cagtagatga tattaaattt aaaggcaatc ctgctttgat tgaaataatt
     7321 ggaaaagaga ttgatgatat ttatcttaat aaaattaata cgatttcttt atctcctgat
     7381 tccataaaaa gaaacagtga tatgaaaatt gtatatacac ctattcacgg aacaactgta
     7441 cgtttagtgc ctgcagctct taaagcttat ggttttacta acattattaa tgtgcccgaa
     7501 caagatgtgg tgagtggcga ttttccaacc gtatattcac caaatccaga agaaccagca
     7561 gcacttaaaa tggctgtaga aaaaggagaa gaagtcgatg cagatttagt aatggcatcc
     7621 gatcctgatg gcgatagatt gggtatagct gtaaaagatg atacaggaaa attcgtatta
     7681 gtaaatggta atcaaacagc tttattattt acgtattata ttattcgtag atggaaagaa
     7741 ttgggtaaat taaacggcaa acaatatgta gtaaaaacta ttgttactac agaattaatt
     7801 gtagatattg ctaaaaaatt caaattacat tactttgatt gttacacagg ttttaaatgg
     7861 atagcagcca tcattcgtga aaatgaagga aagttacaat atattggtgg aggagaagaa
     7921 agttatgggt atttggctca agattttgtg agagacaaag atgctgtatc tgcttgtacc
     7981 ttgatggcag aaattgcagc atgggctaaa gataatggta aatccttgtt tgaacttatt
     8041 caagatattt atttagaatt tggtttctcc aaagaaaaag gaatttctat tgttaagaaa
     8101 ggaaaaagtg gtgcagaaga aatcaaaaaa attatgtcta atttgcgtag caaaccacca
     8161 aaagaaattg ctggttcaca agtaattgtc ataaaagatt atgatacctt gcgtttaata
     8221 gatgtatctg ctggtactac taatgcgatg gaaatgccag aaacctccaa tgtgcttcaa
     8281 ttttttacag cagatggtac taaaatttct gtgcgtccat ctggtacaga acctaaaatt
     8341 aaattttata tagaagtacg tggagaatta aaaaatagaa aagattatga aaaggcaaat
     8401 caagtggctg atgaaaaaat aaaagccgtg atggcgtctt taaagttaga ttaattttat
     8461 ttttatggta atattgaaat actgattgaa aattttttca gtcagtattt tttttgtata
     8521 aaatgaaaag agatgtatat ttgtgctatt caagcaattg aatgatatca gtaactcatt
     8581 taaattgaat cattatgaaa aaaacactac ttgtcatttt tattactttg ttttcatgtt
     8641 tgtatctttt ttctcaaaat gaagtaatta cggaggaacc gccatcttta cgccctttat
     8701 tggttgatac gacagattgt tgtgtagatt gtgcaatgga aatgcagttt cagtctattg
     8761 aaaaggcatt agcaaataaa gagaatgtac attgtttagt tttatctttt tcttcgccca
     8821 aatatactgc tatacccaag gaggttgctc aatttcagca ccttacttgt ttagatatca
     8881 gttttaatag ggtagcggtt attccagagg agatattgca atgccaacaa ttgtattgtt
     8941 tagatttatc aggaaatcat tatttgcaag atttacctaa gtttttaaaa caattaccta
     9001 atctgaaagt tatacgtcta ttagatatga ctttgtggag tagtgctaaa aaacaacagc
     9061 taaaaaaaga ttttgaaggt atttacctag aatttgagta aaccccgatt tcacatacaa
     9121 tgagagtatc tgaatagtct cattgtatgt tgtaaaaaga agaggtaatg atgttttact
     9181 cttctttttc gttactcatg ggtaaaaaac taatttgatg tatattgttt aagtctgaat
     9241 aatcgtattg gtaaatgccg tctgaggcta tcatcattaa ggtattgtga aaagggataa
     9301 catcatatcc atcaatattg gtgtaatggg ctaattcatt agccataatt gtttgaggat
     9361 tatcagcttt gtatattttc aatccatcat cacagataaa caatgtacca ttatcaattc
     9421 ctaatccttt gggtttggtc atcgtataag atactacttg ttttgggttt tctacatctt
     9481 ttacatcaat tgcaattaat tcattgtcgt cttgtccgca tgcattaccc gaatggatgg
     9541 ttacgtaggc gatatcattt tcaactacta ctgggtcgca tccgaaaacg tgttgaatag
     9601 acgatttaaa aacaggttga gtagggtttt ctacagaata aatgagcaag cctgtgggcg
     9661 tacccataaa catattgttt ttataataaa aaatggtttc tacgtcccaa ccaatgtaga
     9721 tattgtcaac cgcttttaca ggtgctgcac cacttaaatt aatgatactc atattgttgt
     9781 taataactga atacaagtaa tcatcgtgta aactaaatcg tgacatggat ccattaatgc
     9841 cagttgagcc tttttcagcc attataggtg ttgggtcaga cgatgaccac caagggaaac
     9901 cagaaaagat agagttagaa gttttaattg tacgagtggc tattgtccaa ccaacaatta
     9961 cttggtcgct tggtctgtcc cagaccattg atccatctat accaaaacta ggatcttcta
    10021 aaggaggtaa agcttgttca aatacatttt ctagtcgtcc aacaaacaat ggcgtagctg
    10081 gagtagaaat atcaaaccat actaaatcaa tatacgaatc cgcatacaag atatcatctt
    10141 taatggcgat gtcagcattg ccaagaagtt cgatataacc aactacttgt gggtttttgg
    10201 catccgtatt attgataata tgaatgccct tttgaggttc tgacacgaat aaatagcctt
    10261 tgtagaaact aattttgcca tacgatgtaa tagggtgtac atcttgtgta atagcaatag
    10321 aactgcgaaa ttcagtagca tccataaaaa ttggttcatt gactgaatat ttttcggtgt
    10381 tttcagtact cggagcacac gcatataaca gggatgttaa caatgctata actacataaa
    10441 ttactttcat atgtgttgaa tattaaatgg tatgcaatta ttcgttagca attttgccga
    10501 tttttccagc aaatacaatg attcctgtgc ttttttcgcg aatcaagaaa agaaatggtt
    10561 tattagcacg gaattcttta aaaattggaa tagatgtaac cgcaacatct acgatggtaa
    10621 cagctgcagc ttcagttcct tcttcgttta cttcgcagta ggtttgatga ataacacgcg
    10681 aaatatagag ttgagcatca gacgatatgc cagaaaagtc tgcttcctct ggtaaaaaag
    10741 ccttttctat tcctaatttc actaaaatag gattgagttg cattttgttt tgtaccttaa
    10801 agcggggcaa ttgtagtgca attgattctg tgtttaagga acttataatt gatttccatt
    10861 ttgtttttgt taagttagga gctatatcgt taattttttt tccttgtttt ggaagtatca
    10921 ccaacatcga aaaagcccca tttccgtatg ataaatcaat gtattgagca tcatcatctt
    10981 cggtataatc aaaactttct gtttgattca tcatatcaac agtagtagag gtgttatcct
    11041 ctgccgtaaa tgtagcttct tgtgtgtgtt tgctatcaaa tttgtttttc caaagtcctt
    11101 taaaataaat tgcgttaatt aggaaagcaa tagaattatc agagatttga tcaagaggtt
    11161 tttttattaa attatttgtt ttttctgcac accaattgtt gataatatca agcgattcgg
    11221 acttagagaa atctaaacct tgcactttgg cattaaaata aacattgttt ttatcaagaa
    11281 aatcttttaa tatgggaaaa tcctttttat accaaataga atttgccaaa ataagttttg
    11341 tttttttatc taaatttggt aagttattta gcaggatttg ataataccga ttaatttgtt
    11401 catcagttaa tccttggtgt tttaaggctt gtttaatttc ttcttttgtt gcaccattcg
    11461 ctccattcca cgtcattccc attgctatgc ttacgcttaa tggagatata aatacgttgt
    11521 cttcttcagt tgtgctaagt gtttcttgca ggaaatcgaa ggcaaaacta ttttcttcat
    11581 caacacgtgc ctccatgcct tcctctaaag tgatagtttt taaggcattt tcagttggtt
    11641 ctgtcgatgt acacgcattt agtagcatga ttgtacccaa tgcaaataaa gttataactt
    11701 tcataataag tagattttta gcaaattata tacaaatata taacataaac gaattaaaaa
    11761 tcctaattat tgtgttaagt tttaaacaat tttacaaaaa tgtatcgtgt gtttttgacg
    11821 aagtctctac caaagccaat agttcgttca actctcgttt tgtaaagtac cgaaattgtc
    11881 ctttttttaa actacctagc gaaatattga taatgcgaat acgttttaat ccacttacac
    11941 cataatcgag gtattcgcac atacgtcgta tttgcctatt gagaccttgt gtgagaataa
    12001 tggaaaaggt atattcgtta atttttttaa ttttacaagg gttggtaatg gtatcaagaa
    12061 taggaactcc agcactcatt ttaagtaaaa aatcgcttgt aatcggtttg ttaaccgtta
    12121 cgatgtattc tttttcgtgg ttattctttg aacgcaaaat tttattaacg atgtctccat
    12181 cgttggtcag aaaaatcaac ccttcacttg ctttgtctag tctcccgata ggaaatattc
    12241 gtagaggatg atttataaaa tcgataatat tgtctttaat tgatgtatcg gtagtgcaag
    12301 taatacccgg aggtttatta aatgcgatat aaatgcattc aacttctttt gatatacgtt
    12361 taccatcaac tttaatttca tcattttcgt ttacttgcat tcctaaattg ccaattgttc
    12421 catttacaag aacgcggttt tcagcaatta atttgtcggc ttctctacgc gaacaaaaac
    12481 cagtttcgga aatagcttta ttcagtcttt tggcgttttc catgcaatta atttattcgt
    12541 tcatgtgtat atctctaaat cctaagaaat aaagaatgcc atcaagtcca atagttgaga
    12601 tagcttgttg ggcattttct tttactttag gttttgcatg aaaagcaatg cctaatccgg
    12661 ctaaattcag cataggtaaa tcgtttgccc catcgcccac ggctattact tgttcgagtt
    12721 cgattttttc aaattgagca atgagacgca ataattctgc ttttcgtttt ccatccacaa
    12781 tttctccaag atgttttcct gtcagtttac catttacaat ttctaattca ttggcatata
    12841 cataatctac atcaaactgt ttttttaatg cattcccaaa atatgtaaaa cctccagata
    12901 aaatagcaat tttaaaacca tattttttta aaatagtcat cagacgtttt gctccatcca
    12961 taattggcaa attttgagct atttcttcca ttacactttc atctaaccct ttgagtaaag
    13021 ctactcgttt gataaaactt tcgttaaaat caatttctcc tctcattgcc gcttctgtga
    13081 tagcacgtac ttttttccct acacccgctt tttcggcaag ttcatcaatt acttctgttt
    13141 gaataagtgt agagtccata tcgaaacaaa taagcctacg atttcgtctg tacatgtcat
    13201 ctttttgaaa agaaatatca attcctaatt cagatgcata gctcataaat tgagatgata
    13261 gttggtcttt attttttaaa tcaccgcgta cggataattc aatacacgaa cgcacattat
    13321 catttaggtt ttctagcgaa ggtcgtcccg ttaatctttt tatcgagtca atattcagtt
    13381 cttgttctga aatagctttt gtaacgatag atagttgttc tgcagtaatc agtttaccca
    13441 ataatgtaac aatgtatctt ccagagcctt gacgattcac ccattcgtta tattgaggta
    13501 tactaattgg tgtaaaacga acatttagat tgagttcaga acacttaaat aatacttctt
    13561 tcagaatatt ggcagcgtca aaactattgc tcgttttaaa tagtatacct aaggataagg
    13621 tatgatgtat atcagcttgg ccaatatcta agatagtagc attgtatttg cctaaaatcc
    13681 ccgctacagc agaggtaaca ccaggctgat cgtcgcctga aatgtttatt aaaataattt
    13741 cactgtttcc catacgattt ctattgatag aatttcttct gcaaaagtat acatatcatt
    13801 tcatttgtaa aatattctga atataaataa taccgaataa gtaatttcaa ctaaaattaa
    13861 tgtgtttttg aacgctcttc ggatgtgtat tgtagtaatt ctgtaaaaaa gagctgaaat
    13921 tgcctatgta agatatcttt attttctatt aatacatgaa tgcttttatc agcattaatt
    13981 gcttttattt tataggtaga cataatttga agtgagctat gtaaaccttt gatagatttg
    14041 tattgatata atcggtttgt aatcataaaa tgatatatga actttttaat aggtgtaggt
    14101 aaaatggagt agtgttgaat aagatttcca tagaattgcc acgaaaatat ccataaattt
    14161 tta
//
LOCUS       DAOSRP010000043        14005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4432_length_14005_cov_5.467957, whole genome shotgun sequence.
ACCESSION   DAOSRP010000043 DAOSRP010000000
VERSION     DAOSRP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_14005_cov_5.467957"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1139)
                     /locus_tag="PLJ82_03980"
     CDS             complement(189..1139)
                     /locus_tag="PLJ82_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOR40697.1"
                     /translation="MQFLDKYYKWIKIVIMVCAYGYLAYILLHFDNYEDFTSNFTNLN
                     AQKFIFLLVVFCLLPINLFVESLKWKTLVSVMQRIPIRVAYKAVVAGITTGFFTPNRI
                     GEPLGRIMFLDSQNRSQAIALSYVGAFGQSFATFLCGVIAGFLLIFSPAFSAYQNSTG
                     MQMGAVLFLVMFISLYFSLPYLCRKFTKISKFDKVNHLLEAISYISLKRLFVVSALSL
                     LRYTIYVSQYYFLLLCFGVDISMYEAFVAIPINYLLVSITPSIAFAEMGIRGSYAIII
                     IGAFSHNPPAVALAGVTVWLINYVIPMITGSLLLTAKKKL"
     gene            complement(1145..1636)
                     /locus_tag="PLJ82_03985"
     CDS             complement(1145..1636)
                     /locus_tag="PLJ82_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOR40698.1"
                     /translation="MLVRFGKIIRNYYIQFLDFLYPLFKQWMHKGLFYYLASGGINVG
                     TNWIMYFVVHNFVVQKQIVDLGFIAFSPHIASLIITFPFTFLLGFGLSKYVSFAESTV
                     RTKTQFFRYAGIIAFNFLVSYLSMKLLVEWFGIYPTPSNMITTIITSVVSYFSQKHYS
                     FKK"
     gene            1734..2528
                     /gene="rsmA"
                     /locus_tag="PLJ82_03990"
     CDS             1734..2528
                     /gene="rsmA"
                     /locus_tag="PLJ82_03990"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOR40699.1"
                     /translation="MSYVKPKKHLGQHFLTDQHVARSIAETLRISEPTKVLEIGPGTG
                     VLTQYLLENELIDLKVIEIDGESVEYLQKEFPALHNRIFFQDFLTTDIPQLYGSETFS
                     VIGNFPYNISSQIFFKILENKHQIPCVCGMLQKEVAERLASKPGKKTYGILSVLLQAY
                     YDVEYLFTVPPTVFNPPPKVNSAVIRVTRNATQQLECNETLFKTVIKTAFNQRRKTMR
                     NSLKGIITVSSPIVTESIFDKRPEQLSVNEFVTLTNLIEPYIQSQH"
     gene            2630..3904
                     /gene="purD"
                     /locus_tag="PLJ82_03995"
     CDS             2630..3904
                     /gene="purD"
                     /locus_tag="PLJ82_03995"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOR40700.1"
                     /translation="MKHTILLLGSGGREHAIAWKLTQSTKLNRLFIAPGNAGTAQLGE
                     NISINPEDFVAVQTFVEKNDVTMVIVGPEAPLVAGIVDFFKATPTIAHIPVLGPSKYA
                     AQMEGSKEFAKEFMKRHAIPTAGYLSVTKDNLAQGFAFLETLKAPYVLKADGLAAGKG
                     VLIISDLNEAKTELAAMLDGKFGKASSTVVIEEFLDGIECSVFVLTDGDKYVILPEAK
                     DYKRIGEGDTGPNTGGMGAISPVSFANATFMQQVEERIIRPTINGFRTENIDYKGFVF
                     FGLIKVNNEPYVIEYNARMGDPETEAVVLRLQNDLLTLVEATFDGTLDKQVMKFDTRF
                     AVTVMLVAGGYPGDYQKGDVITGCELVEDSIAFHAGTTLQNNDIVTNGGRVIAISSYG
                     NTKEEALACSFKNAHKINYKGKYFRSDIGFDL"
     gene            3901..4884
                     /locus_tag="PLJ82_04000"
     CDS             3901..4884
                     /locus_tag="PLJ82_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40701.1"
                     /translation="MNWREERHQILAPWSIFLITGVFAIAFWIKSFLSYDMVNFPTEK
                     DILYYYLTQIIPFDSFLAKIIAFLLVVFIAVITFFANNKYFFIKNKSALIIMFMVLLM
                     GLNDPSHNLNPGIIGLTFLLFAFIRFLGMHGKLHTGARSFDIGLYISIGSLFYDRMLF
                     FIPLFYIGFYIYQQLHIKNILATFFGITFPYLLTGGMCFLFDDFDNFVQRIPFSISHT
                     TDTHIGHIIYYGLLGLVTLLAFFNFILKYSNEKIKTRNTIFFIYLLLLGTIVSIFFSN
                     HLFLFELPFISFFLSIVFAHFFANNTSHFSRFIFYAFWTIGIIWFLVSFIL"
     gene            4886..5344
                     /locus_tag="PLJ82_04005"
     CDS             4886..5344
                     /locus_tag="PLJ82_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOR40702.1"
                     /translation="MSEILYFLTEYGYIGLFFAAFLAATILPFSSEVVFTGLLYSTQS
                     DVWLCILAATLGNWLGGITCYWLGRLGKTEWISRYLRVPPDKVTHWQNKIQGKAALAA
                     FFSFLPGIGDLIAIAAGYLRANFWLTSTSILIGKFLRFVVWVYITYWYWA"
     gene            5567..6022
                     /gene="rplM"
                     /locus_tag="PLJ82_04010"
     CDS             5567..6022
                     /gene="rplM"
                     /locus_tag="PLJ82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR40703.1"
                     /translation="MNTLSYKTISANKATVQKEWVVVDATDQVLGRMCSKVAKLLRGK
                     YKPSFTPHVDCGDNVIIINADKVKMTGNKWTDKVYLRYTGYPGGQREMTPQRLQAKGS
                     DKLFNKVLKGMLPKNRLGDKLLGNVYIYDGAEHPHQAQTPKTIDLNVLK"
     gene            6028..6414
                     /gene="rpsI"
                     /locus_tag="PLJ82_04015"
     CDS             6028..6414
                     /gene="rpsI"
                     /locus_tag="PLJ82_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR40704.1"
                     /translation="MEITNAIGRRKAAVARVFVREGKGNIVINKRGIDSYFPSGILQY
                     IVKQPLAKLDVVEKYDIQVNLNGGGFKGQAEALRLAIARALVKINPEDKPALKSEGFM
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            6572..7411
                     /gene="rpsB"
                     /locus_tag="PLJ82_04020"
     CDS             6572..7411
                     /gene="rpsB"
                     /locus_tag="PLJ82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOR40705.1"
                     /translation="MSRTNFQDLLDAGAHFGHLKRKWNPAMSPYIFMEHNGIHIIDLH
                     KTVVKIDEAAEVLFKIAKQGKKVLFVATKKQAKDIIADKASSVNMPYITERWAGGMLT
                     NFPTIRKAVKKMSVIDKMATDGTFENLSKREKLQISRQREKLEKNLGSIADLTRLPAA
                     LFVVDVMKEQIAVKEANRLGIPVFGIVDTNSNPNNIDYVIPANDDAAKSIEIILKAVV
                     EAIQEGLQERKNDKLELEASEVPVDKMKKSKAPSKVRTKRGIDESALNANVADKFLKG
                     EKE"
     gene            7521..8345
                     /gene="tsf"
                     /locus_tag="PLJ82_04025"
     CDS             7521..8345
                     /gene="tsf"
                     /locus_tag="PLJ82_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOR40706.1"
                     /translation="MAVTMAEISKLRTMTGAGMMDCKNALTESNGDFDKAVEIIRKKG
                     QAVAAKRSDREASEGCVLAASKEAFAACVALKCETDFVAKNADFIALTQTILDIVMDK
                     QPTTLEEVLALPMGNGTIASSITDRSGITGEKMELGYYEWVKGASTVSYIHPGNKLAT
                     IAAFNLAGTDQTVSRDVAMQIAAMNPISLNRDSVPANVIATELEIGRDKARQEGKPEA
                     MLDKIAEGRLNKFYQESTLLEQAFIKDGKITVQNYLKQANKDLTVTDFKRVTLNQE"
     gene            complement(8418..10457)
                     /gene="metG"
                     /locus_tag="PLJ82_04030"
     CDS             complement(8418..10457)
                     /gene="metG"
                     /locus_tag="PLJ82_04030"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR40707.1"
                     /translation="MEKKFKRTTITSALPYANGPVHIGHLAGVYIPADIYARYLRLKG
                     QDVLFVGGSDEHGVPITLKAKKEGVTPQDIVDRYHTIIKQSFEDFGISFDVYSRTTSK
                     THHEVASTFFKTLYDKGEFVEKTSEQYFDQEAQQFLADRYIIGTCPHCKNTQAYGDQC
                     EACGTSLSPMDLIDPKSTISGSTPILKETKHWYLPLDKHEAWLRQWILEDHKEWKSNV
                     YGQCKSWLDMGLQPRAVSRDLDWGIPVPVAGAEGKVLYVWFDAPIGYISNTKELLPDT
                     WELWWKDEQTKLVHFIGKDNIVFHCIVFPAMLKAEGSFILPENVPANEFLNLEGDKIS
                     TSRNWAVWLHEYLIDFPAKQDVLRYTLTANAPETKDNDFTWKDLQARNNSELVAIYGN
                     FVNRTLVLTHKYFEGKVPALGELTDIEKSVLKEFEDVQHRVESLLNTYRFRDAQKEAM
                     NLARIGNKYLAETEPWKLAKTDMERVATILNVSLQISANLSIAFEPFLPFSSQKLRSL
                     LGLQTCDWSALGSINLLSEGQTIQSPELLFEKIEDDAIEAQVQRLLDTKKANEVADYK
                     PQPIKENVAFDDFMKLDIRVGTVLDCQKVPKADKLLQFKIEDGMGTRTIVSGIAQYYQ
                     PADLIGKQVCFIANFEPRKLKGIASEGMILSAEDSDGRLVVISPSDLVKNGVKIS"
     gene            10687..11955
                     /locus_tag="PLJ82_04035"
     CDS             10687..11955
                     /locus_tag="PLJ82_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOR40708.1"
                     /translation="MRQFLRSSFFKHTATLLTANSLSQAIALLAYPLLTRIYAPDDFG
                     LFNLFLSIGGILAIIGTGNYHYAIVLPKEETKALACVKLSFILNLIVVGISSFFLLFS
                     SQIGKLFNSESLGEYLYLLPIFVLLTATWNILNYWFTRHQCYKPIAFYQISNSLGSSI
                     LKYMLGIMEFLRGGLLIGTILAQVIAFAVGFIVAWKKGLSRTLHIQRQEIVETATTYK
                     NFPFYSLPHSLINTLGGSLPIILLAPFFSLAEIGFFGMALTLAFRPINIISSSLHQVF
                     YPITTQKVNDNDSIVPFFKRYFLRTFTLLIPLFCVLFFFLPMLTEWLLGNGWHQSGKY
                     IQLMLPWLALSAAVAPITYLADIFSQQRIALLLEILSFIVRFCALMIGIWQQNFYLAI
                     AYYCVASALIILVQLLWYWHLVKQYEIKRI"
     gene            11958..13121
                     /locus_tag="PLJ82_04040"
     CDS             11958..13121
                     /locus_tag="PLJ82_04040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40709.1"
                     /translation="MRVLQLPSWYIPEGGMFCTEQSIALKQAGVEVHILANVALPWRK
                     YKSRIYSFPRKPFFTEENGITLLRYYAKRIPFADMWNMKLWVRNTLRLYDMYFEKFGH
                     PDIIHTHSSMWAGYAAALIKQRYGVPYVITEHRGRFSENSIESDELFPLSYHPFLYQA
                     FENAHSIIIVSEQLRKKVALYAGENVPIYTISNVVNTDFYTEKKEQNPIRPFTFINAN
                     SYHFAKAYDVLLDAFEKVYAQYPETTLLLLGNGFDDAQFVVQLNKLRSKNKIEFKGYQ
                     PPEGVLHYLHKSDSFVLSSRIEAQPVAILEAMSCGLPIVCTEVVPSEIVTPTIGVRCK
                     TDNADALAEAMIYMIEHHASFSSTDIRQFAVSVCSQKAVASQILAVYKQIIHG"
     gene            13114..>14005
                     /locus_tag="PLJ82_04045"
     CDS             13114..>14005
                     /locus_tag="PLJ82_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOR40710.1"
                     /translation="MVKLDKHNPISWTSANGVWVAGYCFDTEGNFYEKESLIALLQQI
                     TENHLESFVRSLNGIFACIIERDSETIIIMDKTRLIPLFYAIENNQVIVSNNPYTLLP
                     TSPVVDTSALNEYLYSSVVLDEKTLIKGIFQVQPSSYIICKNNHLDIHHYYTFAVKKH
                     EIAVAKDYNQQFETVLNDVFHRLLTSCKGKQVVVPLSGGYDSRLIVCILAKLGYKNVL
                     CYTVGRPNNPELIIAQKVAKRLGYKHVFISNETPSYLDDNTFEAYYRYSGYFTNFFWM
                     YEYIGVKTLTEQNIIEKDAIF"
BASE COUNT         4671 a         2514 c         2453 g         4367 t
ORIGIN      
        1 attagccgta gttaccgtat atcctttaga ctctaaaaat agaatatgag accgcaataa
       61 gtcaatttca tcatctaccc ataaaatatg tatatttgtc ataaatggtt acgtttggtg
      121 atttgttctt ttttacaaag atatgacatt tacaggtatt cttcaattat ttaagcagta
      181 ttatctggtt aaagtttctt ttttgcggtt aaaagaagtg aaccagttat catgggaata
      241 acataattta ttagccaaac cgtaactcca gccaaagcta ctgcaggagg attgtgtgaa
      301 aaagctccta tgataataat agcatacgat cctctaatgc ccatttcggc aaatgcgatt
      361 gaaggtgtta tagatacaag caaataatta attggtatag cgacaaaagc ttcgtacatt
      421 gaaatatcta ccccgaaaca caataataaa aaataatact gagaaacata tatcgtatac
      481 ctaagtaaag aaagtgctga aacaacaaaa agtctcttta gagaaatata ggatatagct
      541 tcaagcaaat gattcacttt atcaaattta ctaatctttg taaattttct acacaaatac
      601 ggtagcgaaa aatacaaaga tataaacatc actaaaaata atacagcacc catttgcata
      661 cccgtagaat tttgatacgc actaaatgct ggtgaaaaaa tcaacaaaaa acctgcaatg
      721 actccacata aaaacgtagc aaaactttga ccaaaagcac caacgtagga aagtgctatt
      781 gcttgcgaac gattttgcga atccaaaaac ataatccgac caagaggttc tcctattcta
      841 tttggggtaa aaaacccagt ggtgatacca gctacaacag ccttgtaagc tacccgaata
      901 ggaattcttt gcataacaga aaccaatgtt ttccatttca gagattctac aaatagatta
      961 attggcaata ggcagaaaac aacaagcaag aatataaact tttgagcgtt taagtttgta
     1021 aaatttgaag taaaatcttc ataattatca aaatggagta aaatatatgc caaatatcca
     1081 taggcacaca ccataatcac tattttaatc cacttgtaat atttatctaa aaattgcatc
     1141 tctcttactt tttaaacgaa tagtgttttt gagaaaaata actaaccaca ctggttatga
     1201 tagttgtaat catattagat ggcgttggat atataccaaa ccattctact aataatttca
     1261 tagaaagata acttaccaag aagttaaacg caattatacc agcatatcga aaaaattgcg
     1321 tcttcgttcg tactgtcgat tcggcaaaac ttacatattt cgataatcca aaacccagta
     1381 aaaaggtaaa cggaaaagta ataatcaaac tcgcaatatg cggactaaag gcaataaatc
     1441 ctaagtcaac tatctgtttt tgcaccacaa agttatgcac tacaaaatac ataatccagt
     1501 tagtgcccac attaatacca cccgaagcta aataataaaa cagccctttg tgcatccact
     1561 gtttaaataa cggatacaaa aaatcgagaa attgaatgta ataattgcgt attattttgc
     1621 caaaacgaac taacatactt ttaggtactt gctttttgat tatctttgca aagataaact
     1681 tttttaaaac gaaagcatat ttttgctttt attatttgct aaagatatac actatgtcat
     1741 acgtaaaacc aaaaaaacac cttggacaac atttccttac cgaccaacac gtagctcgta
     1801 gcattgccga aacattgcga attagtgagc ccacaaaagt actcgaaata ggtcctggca
     1861 caggcgtatt gacacagtat ttattggaaa acgaactcat tgatttgaaa gtaattgaga
     1921 tagatggaga gtctgttgaa tacttacaaa aagaatttcc tgccttgcat aaccgcatat
     1981 tttttcaaga tttcttgaca acagatatac ctcagcttta cggatcagaa actttctctg
     2041 ttataggcaa tttcccgtat aatatatcta gtcagatatt ttttaaaata cttgaaaaca
     2101 aacaccaaat cccctgtgtg tgtggtatgt tgcaaaagga agtggcggaa cgattggcgt
     2161 cgaaaccagg gaaaaagaca tatggaattt taagtgtttt attacaagcc tactacgatg
     2221 tggagtattt atttacggta cctcctactg tgtttaaccc accgccaaaa gtcaactcgg
     2281 cagttatacg tgttacacgc aacgctactc aacaactaga atgcaacgag accttattta
     2341 aaactgttat caaaacagct ttcaatcaac gaagaaaaac aatgcgaaat tcgctaaaag
     2401 gcatcataac agtatcttct cctattgtaa cagaaagcat attcgacaaa cgtccagagc
     2461 aactttctgt aaacgaattt gttacactta ctaatttaat tgagccatat attcaaagtc
     2521 aacactaaca catatgtata catactaatt acaagaaaac aattagtatc taaaaaagaa
     2581 aaaaattgta cctttgtaac gtcaacacat aaagcaaatt tttacataca tgaaacacac
     2641 aatactactt ttgggatcag gaggcagaga acacgccatc gcttggaaat taactcaaag
     2701 tacaaaactt aatcggttat ttatcgcccc tggcaatgca ggtaccgctc aacttggaga
     2761 aaacatctcc ataaaccccg aagactttgt ggcagtacaa acatttgtag aaaaaaatga
     2821 tgttaccatg gtaatcgttg gtccagaagc tcctttggtt gcaggtattg tagatttttt
     2881 taaagcaact cctacgatag cacatatacc cgttcttggt ccttcaaaat acgctgcaca
     2941 gatggaaggc agtaaagaat ttgccaaaga atttatgaaa agacatgcta taccaacagc
     3001 tggatatctt tcggttacaa aagacaattt ggctcaaggg tttgcatttt tggaaacatt
     3061 aaaagccccc tatgtgttaa aagcagatgg attagcagct gggaaagggg ttttgattat
     3121 cagcgactta aatgaagcaa aaacagaatt agcagctatg cttgatggca aatttggcaa
     3181 agcaagctca acagtagtga tagaagaatt tttagatgga atcgaatgtt ccgtgtttgt
     3241 actaactgac ggagataaat atgttattct tcccgaagcc aaagactaca aacgaatagg
     3301 cgaaggcgat acaggtccaa atacaggagg tatgggggca atttctccgg taagctttgc
     3361 caacgctacc tttatgcaac aagtagaaga acgaattatt cgtccgacta taaacggatt
     3421 tcgcacagaa aacattgact acaaaggatt tgtgtttttt ggtttgataa aagtaaacaa
     3481 tgaaccttac gtaatagaat acaatgcacg tatgggtgat cccgagacag aagcagtggt
     3541 gctacgctta caaaatgatt tacttacact agttgaagca acgtttgatg gcactttaga
     3601 taaacaagta atgaaatttg atacccgatt tgctgttact gtaatgttag tagcaggagg
     3661 atatcctggc gattatcaaa aaggcgatgt gattacaggt tgtgaacttg ttgaagacag
     3721 tattgcattt catgcaggca ccacacttca aaacaacgat attgtaacca acggaggacg
     3781 tgtaatagca ataagttcat acggaaatac gaaagaagaa gcattggctt gctctttcaa
     3841 aaatgcacat aaaatcaatt acaaaggaaa atattttcgt tcagacatag gatttgattt
     3901 atgaattgga gagaagagcg acatcaaata ctagcaccgt ggtccatatt tttgattacg
     3961 ggggtttttg ctattgcatt ttggatcaag tctttcctgt catatgatat ggtaaatttt
     4021 cctactgaaa aagacatcct atattattat cttactcaaa tcattccttt tgattcattt
     4081 ttagccaaaa ttattgcgtt tttgctagtt gttttcattg cagtaattac gttttttgcc
     4141 aacaataaat atttctttat aaaaaataaa tcagccttaa tcattatgtt tatggttttg
     4201 ttgatgggac taaacgaccc ttcgcacaac cttaacccag gtattattgg cttaaccttt
     4261 cttctgtttg cctttatacg gtttttgggc atgcatggca aattacatac aggagcacgt
     4321 tcttttgata ttggattata tatttcaatc ggaagtttat tttatgatag aatgctcttt
     4381 tttattccct tgttttacat cggtttttat atttaccaac aattacacat caaaaatata
     4441 ctagctacct tttttggaat tacttttcct tatttactca caggcggtat gtgtttttta
     4501 tttgatgatt ttgataattt tgttcaacgt attcccttta gcatatcaca taccacagac
     4561 acacacattg gacacattat ttactatgga ttgttggggt tagtaacgtt gctcgctttc
     4621 tttaatttta tactaaaata cagtaacgaa aaaatcaaaa cacgtaacac catctttttt
     4681 atttaccttc ttctattagg tactattgta agtatatttt tttctaacca tctttttttg
     4741 tttgagcttc ctttcatttc gttcttcttg agtattgttt ttgcacattt ttttgcaaat
     4801 aatacttctc atttttctcg atttattttt tatgcatttt ggactattgg tattatttgg
     4861 tttttagtct catttatatt ataaaatgtc agaaatactt tattttttaa ctgaatatgg
     4921 ttatatcgga cttttttttg ccgctttttt agctgcaacc atcttgccat ttagttctga
     4981 ggtagtattt acgggattac tatatagtac ccaatcggat gtttggctat gtattttagc
     5041 tgctacctta ggaaattggt taggagggat tacctgttat tggctaggga gattaggaaa
     5101 aaccgagtgg attagtcgct atttacgagt tccaccagac aaagtaacac attggcaaaa
     5161 taaaatacaa ggcaaagccg ctttagctgc cttcttctct tttttaccag gtattggcga
     5221 tttaatagcc atagcagcag gttatttacg tgctaatttt tggcttacca gcacatctat
     5281 attaatagga aaatttttgc gttttgtggt ctgggtgtat attacttatt ggtattgggc
     5341 atagtaatgc aaaaaaacac cttcccctaa aacaaacaaa aacacacaaa tgtaaataaa
     5401 attgttgaaa agtttttctg tttcagaaaa atacagtatt tttgcagtcc gattattcct
     5461 ccacgcacgt aagtgtttag tttttagcga gtaagaatct tttttagcct gaagacctta
     5521 gtttgcgaga aacgaaagag tgtaaaattt ttaataaatt attattgtga ataccttaag
     5581 ttacaaaact atttctgcta ataaagccac tgtacaaaaa gagtgggttg tagtagatgc
     5641 taccgaccaa gtgttgggtc gtatgtgttc taaagtagca aaattgttga gaggaaagta
     5701 taaaccatct ttcactcctc acgttgattg cggtgataat gtaatcatta tcaatgcaga
     5761 taaagtaaaa atgactggca acaaatggac agataaagtc tatcttcgct acacaggata
     5821 tccaggtggt caacgcgaaa tgactcctca acgtttgcaa gcaaaaggct ctgacaaact
     5881 tttcaataaa gtactgaaag gtatgttgcc caaaaatcgc ttgggagata aattattagg
     5941 caatgtgtat atttatgatg gagcagagca tccacatcaa gcacaaactc caaaaacaat
     6001 tgatttaaac gttcttaaat aggaaatatg gaaattacaa atgcaatagg aagaagaaaa
     6061 gccgctgtag ctcgcgtatt tgtgagagaa ggtaaaggca atattgttat caataaaaga
     6121 ggtattgatt catattttcc atcaggaatt ttacagtata tcgtaaaaca accccttgct
     6181 aaattagatg tggtagaaaa atacgatatt caggtaaact taaatggtgg tggttttaaa
     6241 ggacaagcag aagcattgcg tttggcaatc gctcgtgcat tggttaaaat caacccagaa
     6301 gacaaacctg cattgaaatc agaaggcttc atgacccgtg atccacgcga agtggaacgt
     6361 aaaaaaccag gtcgtccaaa agctcgcaaa agattccaat tcagcaaacg ttaatattcg
     6421 aatactcgtt attacctggt atttggcaag ggtttatatc caccaacagc cagtaatacg
     6481 gtaggtttag tatctaaatt atagggactt tgttgcacaa actaccctat aattgaataa
     6541 aagcagaaag taaacaacaa aaaacaaaca catgtcaaga acaaattttc aagatttatt
     6601 agatgcaggt gcacattttg gtcacctaaa aagaaagtgg aatccagcaa tgtctccata
     6661 catttttatg gaacacaatg gtattcacat cattgattta cacaaaacag ttgtaaaaat
     6721 cgatgaagca gccgaagttt tattcaaaat tgccaaacaa ggtaaaaaag tattgtttgt
     6781 agcaacaaaa aaacaagcaa aagatatcat tgctgacaaa gcaagttctg taaatatgcc
     6841 ttatatcaca gaacgttggg ctggtggtat gcttaccaac ttccctacta tccgtaaagc
     6901 tgtgaaaaaa atgtctgtta tagataaaat ggctactgat ggtacatttg aaaatctttc
     6961 taaaagagaa aaattacaaa tctctcgtca aagagaaaaa cttgaaaaaa acttaggtag
     7021 tattgctgat ttaacacgtt tacctgcagc tctatttgta gttgacgtaa tgaaagagca
     7081 aattgcagta aaagaagcta atcgtttggg tattcctgtt tttggaattg tagatacaaa
     7141 ctctaacccc aacaacattg attatgttat tcctgcaaat gatgatgcag ctaaatcaat
     7201 tgagattatc ttaaaagcag tggttgaagc aattcaagaa ggcttacaag aaagaaaaaa
     7261 tgataagcta gaattggaag catctgaagt gcctgttgat aaaatgaaaa aatctaaagc
     7321 tccttcaaaa gtaagaacga agagaggtat tgatgagtca gcacttaatg caaatgttgc
     7381 agataaattc ttaaaaggag aaaaagagta atattttttc ttttatattt atgaataggt
     7441 tacaactcaa actctttctt taaagaagtg gtggcggttg taactttttt tttgacacta
     7501 aacaaataaa acatactatt atggcagtaa caatggcaga aatatccaaa ttacgcacaa
     7561 tgactggtgc gggaatgatg gattgcaaaa atgctcttac agagtcgaac ggtgatttcg
     7621 acaaagcagt agaaataatt agaaaaaaag gacaagcagt agccgcaaaa cgtagcgaca
     7681 gagaagcttc tgaaggttgt gtacttgcag catctaaaga agcgtttgca gcatgcgtag
     7741 ctctaaaatg cgaaacagat tttgtggcta aaaatgcaga ttttatagca ttaacgcaaa
     7801 caattcttga tatcgtgatg gacaaacaac caacaaccct tgaagaagtg ttggctttac
     7861 caatgggtaa tggtactatt gcctcatcta ttacagatag aagtggtatt acaggcgaaa
     7921 aaatggagct tggttattac gaatgggtaa aaggtgctag cactgtatcg tacatacacc
     7981 caggaaataa attagctact attgcagctt ttaaccttgc aggaactgac caaacagtat
     8041 ctcgtgatgt ggctatgcaa attgcggcaa tgaatccaat ttctttaaat agagattctg
     8101 tacctgcaaa tgttattgct actgaattag aaattggtcg tgataaagca cgccaagaag
     8161 gtaaaccaga agctatgctt gataaaattg cagaaggacg tttaaataaa ttctaccaag
     8221 aatctacttt attggaacaa gcttttatca aagatggtaa aataactgta caaaactatt
     8281 taaaacaagc aaacaaagat ttaactgtta ccgatttcaa acgtgtaaca ttgaatcaag
     8341 agtaagctct tgtgataata aaaataaaaa atggagactg aaaaatcagc ctccattttt
     8401 tatagagttg tactgtacta tgaaattttt acgccgtttt tcactaaatc gctcggcgaa
     8461 attaccacca aacgtccatc gctatcttct gccgataaaa tcattccttc gctggcaata
     8521 cctttaagtt ttcgtggttc gaaattggca ataaagcata cttgttttcc tattaaatca
     8581 gcaggttggt aatactgagc aatacccgac acaatcgtac gtgtacccat accatcttca
     8641 attttaaatt gcaagagctt atctgctttg ggtacttttt ggcaatctaa cactgtgccg
     8701 acacgtatat ctaatttcat aaaatcgtca aacgccacgt tttctttaat tggttgtggt
     8761 ttataatcag ctacttcatt cgccttttta gtatctaata gtcgttgtac ctgcgcttcg
     8821 atagcatcat cttcaatctt ttcaaacaac aactcgggcg attgtatcgt ttgaccctct
     8881 gagagtaagt taatagaacc taaggcactc caatcacatg tttgcaaacc aagcaatgaa
     8941 cgtaattttt gtgaactaaa aggtaaaaat ggttcgaatg caattgacaa gttagcactt
     9001 atttgtaaag aaacattcaa gatagttgct accctttcca tatccgtttt ggcaagtttc
     9061 caaggttcgg tttcggctaa atatttgttt ccaatgcgtg ctaaattcat tgcttctttt
     9121 tgagcatctc taaatcgata agtattgagc aaggactcaa ccctatgttg cacatcctca
     9181 aactctttca aaaccgactt ctctatatca gtaagctctc ccaaggcggg cacttttcct
     9241 tcaaaatatt tatgcgtaag caccaatgtt cggtttacaa aattgccata aatagccact
     9301 aattcgctat tattgcgagc ttgtaaatcc ttccaagtaa aatcattgtc ttttgtttcg
     9361 ggagcattgg ctgtaagtgt atatcgtaat acatcttgtt tggctggaaa atcgattaaa
     9421 tattcgtgta accaaacagc ccaattgcgt gaagttgata ttttatctcc ctcaaggttt
     9481 aagaactcat tggctggaac attttcaggt aaaataaatg acccttcagc ttttaacata
     9541 gctggaaaca cgatacagtg aaacacaatg ttgtctttcc cgataaaatg tactagttta
     9601 gtttgttcat ctttccacca cagttcccat gtatctggca acaattcttt ggtattagaa
     9661 atataaccaa taggagcatc aaaccacaca tacaaaacct tgccctctgc tcctgcaact
     9721 ggtacaggaa ttccccaatc taagtcgcga cttacggcac gtggttgcaa acccatatcg
     9781 agccagcttt tacactgtcc atacacattg cttttccatt ctttgtggtc ttcgagtatc
     9841 cattgacgca accaagcttc gtgtttatcg agtggtaaat accaatgttt tgtttctttg
     9901 agaattggcg tagaaccaga gatggttgat ttagggtcaa ttaaatccat aggactgagc
     9961 gatgtgccac aggcttcaca ctgatcacca taagcttgtg tatttttgca atgcggacat
    10021 gtgccaataa tataacggtc ggctaaaaac tgctgtgctt cttggtcgaa atactgttca
    10081 gaagtttttt ctacaaactc acctttatca tacagcgttt taaaaaatgt agaagctact
    10141 tcgtggtgcg tttttgatgt agtgcgagaa tagacatcga acgaaatacc aaaatcttcg
    10201 aatgattgtt taataatagt atgataccta tccacaatat cctgaggcgt tacgccctct
    10261 ttttttgctt tgagcgtaat gggcacacca tgctcatcag aaccaccaac gaataaaaca
    10321 tcttgcccct ttaggcgaag atagcgggca taaatatcag caggaatata cactccagcc
    10381 aaatgtccga tatgaacagg accgttggca taaggcaatg cagaagtaat ggtagtacgt
    10441 ttaaatttct tttccatatc tgtgctaagt ctgattatca agtttgtgtt tgagtaagaa
    10501 tgcaaagtta catatttttc gtggtttctt tgcctatgtt tcgtaaatat ttaaggtaat
    10561 caaaaccaat accatgctac ctaattcaaa gaaaaagata atcgaaaaga aatttggcaa
    10621 gataggcaat aaaaatgtat ctttgttcat cggtatggat tatttttcgt gattaaatac
    10681 aaacggatgc gtcagtttct taggtcatcg ttttttaaac acacagcaac attgcttact
    10741 gccaatagct tatcacaagc cattgcctta ttggcatatc ctttactgac acgcatttat
    10801 gcccctgacg atttcggatt gttcaatcta tttttaagta tcggcggaat attggcaatt
    10861 attggaactg ggaactacca ttatgccatt gtgttgccaa aagaagaaac aaaagcccta
    10921 gcttgtgtaa aattatcctt tatactgaac cttattgtag taggtataag tagtttcttc
    10981 ctactctttt catcacagat tggcaaatta tttaattctg agtcattagg agaatacctg
    11041 tatttactcc ccatattcgt attattgacc gcaacatgga acattcttaa ctactggttt
    11101 acacgacacc aatgctacaa acccatagca ttttatcaaa ttagtaacag cttgggttct
    11161 tcgatactaa aatatatgtt aggtatcatg gagtttttac gtggtggttt attaattggc
    11221 actatacttg cacaagtaat tgcttttgcc gtaggtttta tagtggcatg gaaaaaaggt
    11281 ttgtcaagaa ctttgcatat acaacgacaa gaaatagtag aaacggcaac aacatataaa
    11341 aatttcccat tctattcttt accacacagc ttaatcaaca ccttaggagg aagtttgcca
    11401 attatccttt tagctccatt tttttcatta gcagaaatag gtttttttgg catggcactt
    11461 acgcttgcat ttcgcccaat caatattatt tctagttcgc tacatcaagt attctatcct
    11521 attaccaccc aaaaggtaaa cgacaatgac tccattgtgc cattttttaa acgatacttt
    11581 ctacgaacat ttaccttgct tataccctta ttttgtgtgc tttttttctt tttacctatg
    11641 cttaccgaat ggttattggg taatggttgg catcaaagcg gaaaatatat tcagttgatg
    11701 ctaccttggt tagccctatc agcagcagtt gcacccatta catatttagc agatattttt
    11761 tctcaacaaa gaattgcctt gttgcttgaa attctttcat ttattgtgcg attttgtgca
    11821 ttaatgattg gaatatggca acaaaatttc tatcttgcca tcgcatatta ttgtgttgca
    11881 agtgctttaa ttattcttgt tcaactactt tggtattggc atctggtaaa acagtacgaa
    11941 attaaacgaa tttgattatg agagtgttac agttaccatc ttggtatatc cccgaaggag
    12001 ggatgttttg tacagaacaa agcattgctc taaaacaagc tggcgttgag gtacatatct
    12061 tagctaatgt ggcacttcct tggcgaaaat acaaatctcg catttattcg tttcccagaa
    12121 aacctttttt taccgaagaa aatggtatta ctctactgcg ttattacgcc aaaagaatac
    12181 cttttgctga tatgtggaat atgaaactat gggttagaaa tacattgcga ctatatgata
    12241 tgtattttga gaaatttggt catccagata ttattcacac acacagtagt atgtgggctg
    12301 gatacgctgc tgcacttatc aagcaacgtt atggcgttcc gtacgtaatt accgaacata
    12361 gaggtcgttt ttctgaaaat tctattgaat ctgacgagct atttccgtta agctatcatc
    12421 cttttttgta ccaagcgttc gaaaatgcac atagtatcat cattgtatcg gaacaactgc
    12481 gtaagaaagt ggctctttat gctggagaga atgttcctat ttatacaatt tcaaatgttg
    12541 taaatacaga tttctatacc gaaaagaaag aacaaaatcc tatccgacca ttcaccttta
    12601 tcaatgccaa ttcttatcat tttgccaaag cctacgatgt attgctagat gcttttgaaa
    12661 aagtatatgc acaatatcct gaaacaactt tactgttatt gggcaatgga tttgatgatg
    12721 ctcaatttgt agttcagctc aacaaattac gctcaaaaaa taaaatagaa tttaagggat
    12781 accaaccacc tgaaggcgta ttgcattact tacataaaag cgattcgttt gttttatcaa
    12841 gccgaataga ggcacaacct gtggctattt tagaagccat gagctgtgga ttgcctattg
    12901 tgtgtacaga ggtggtgcct tccgaaattg ttacccctac tatcggtgta cgttgcaaaa
    12961 cagacaatgc agatgcacta gcagaggcaa tgatctacat gattgagcat catgccagtt
    13021 tctcatctac cgatattagg caatttgccg tttctgtgtg tagccaaaaa gctgtagctt
    13081 cacaaatctt ggcggtgtat aaacaaatta ttcatggtta aacttgacaa acataatccc
    13141 atctcgtgga caagtgccaa tggcgtttgg gtagctggct attgttttga tacagaagga
    13201 aatttttatg aaaaagaatc acttattgcg ttactccaac aaatcacaga aaatcattta
    13261 gaatcattcg tacgttcgct taatggaata tttgcctgca tcattgaacg agatagcgaa
    13321 acgattatta tcatggataa aacacgcttg attcctcttt tttatgccat agaaaataat
    13381 caagtaatcg tatccaataa tccatataca ttgcttccca catcgccagt tgttgatacc
    13441 tctgcactta atgaatacct gtattcttcg gttgtgttgg atgaaaaaac actgattaaa
    13501 gggatatttc aagtgcaacc atccagttat attatttgta agaataacca ccttgatata
    13561 catcattatt acacttttgc agtaaaaaaa cacgagatag ctgtagcaaa agattacaat
    13621 cagcaatttg aaacggtgct gaacgatgtt tttcatcgat tacttacctc gtgcaaaggg
    13681 aaacaagtag ttgtaccctt gagtggtggc tacgattctc gcttaattgt atgtatatta
    13741 gccaaacttg ggtataaaaa tgtgctttgc tatacagtag gcagacccaa taatccagag
    13801 ttaataatag cccaaaaagt agcaaaaagg ctaggatata agcatgtatt tatttcgaac
    13861 gaaacaccct cgtacctgga tgacaataca tttgaggctt attatcgtta ctctggttat
    13921 tttaccaatt tcttttggat gtacgaatat ataggggtaa aaacgctgac tgagcaaaac
    13981 atcattgaaa aagatgccat attta
//
LOCUS       DAOSRP010000044        14003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4434_length_14003_cov_4.829581, whole genome shotgun sequence.
ACCESSION   DAOSRP010000044 DAOSRP010000000
VERSION     DAOSRP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_14003_cov_4.829581"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..865
                     /locus_tag="PLJ82_04050"
     CDS             251..865
                     /locus_tag="PLJ82_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOR40711.1"
                     /translation="MITIIDVFSFLGAFAFAISGIRLASKKQFDWFGAYIVGLVTAIG
                     GGTLRDLLLDQPPFWMEQGWYLISTLMALLFVVALGKYVNKLSSSFFVFDTIGLAVFT
                     IAGIEKTLVFGYSYWIAIVMGVMTGCLGGIIRDIFINETPLIFRKEIYAIASIIGGIL
                     YLVSLHVFNLSELISYVIGFSSIVLVRFLAVKYEISLPQLRDSQ"
     gene            870..1679
                     /locus_tag="PLJ82_04055"
     CDS             870..1679
                     /locus_tag="PLJ82_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR40712.1"
                     /translation="MSTQIKFCSLASGSSGNCYYVGNEKHGILIDAGIGVRTIKKRLK
                     DIGVALESILGIFVTHDHFDHIASIGVLGEIHHIPIFSTSEILDGINRCYKVTQKLQH
                     SKRPIAKNKIIEIGSFTIEAFSVSHDASDCVGYTICHGTKKFTIATDLGYINTEAAQH
                     LMNATSFVVESNYDEQTLLENPSYPTYLKERILSNKGHLSNHALAEFLSINYQEHWQH
                     IFLCHLSKENNSPECALSTIDKKIAHQRFNLWANCTLIALPRTTPTEVFII"
     gene            1901..3745
                     /locus_tag="PLJ82_04060"
     CDS             1901..3745
                     /locus_tag="PLJ82_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOR40713.1"
                     /translation="MAEQVKKDNQDNIVIRFSGDSGDGMQLAGTLFANISAMIGNDIS
                     TFPDFPSEIRAPQGTLSGVSGYQVHIGPKVYTPGDKTDVLIAMNPAALKINVKNLKNS
                     SVVVIDTDSFTKKDLEKALFSTSNPFEELGIGNISVIEAPIGSMVKESLTNSGLEGKD
                     VLRCKNMFALGLSCWLFDRPLNLAESLIRTKFAKKPIVAEANIKVLHDGYNFGANTHA
                     SVPTYHIATSTKEKGFYTDVDGNTATAYGLIAAAEKAGMELFLGSYPITPATDILQEL
                     AKHKALGVKTMQMEDEIAGICSAIGASFAGSLAATNTSGPGLALKSEAIGLAVMAELP
                     IVIIDVQRGGPSTGLPTKSEQTDLMQALYGRNGESPVAVVAAKSPTDCFDAAFWASKI
                     AVEHMTPVILLTDGYIANGSGAFKIPELENYPSIQPHYIKKDQQNWKPYTRNEETKVR
                     YWATPGTEGFQHRIGGLEKDYTTSIISTDPDNHQLMVETRQEKINAIANFIPIIDIQG
                     DANADVLIVGWGGTYGHLLSAVNEMNENGKKTAFAHFSFISPLPKNTEEILRKYKKII
                     VCEQNLGQFAGYLRMKIHGLPIYQYNEVTGQPFLVSELVKYFTKIMEE"
     gene            3750..4772
                     /locus_tag="PLJ82_04065"
     CDS             3750..4772
                     /locus_tag="PLJ82_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOR40714.1"
                     /translation="MSELTYKPFQASDYKSAQAVRWCPGCGDHFLLNSLHKAMANIGI
                     PPHQIAVISGIGCSSRLPYYMNTYGFHTIHGRGAAIASGLKVANPKLSVWQITGDGDC
                     LAIGGNHFIHSIRRNMDLNIVLVNNQVYGLTKGQYSPTSDRGYVCKSAPYGTVEDPFR
                     PAELTFGARGTFFARTVDVDSPVAVEVLTAAAKHKGASVVEVLENCIIFNDGTHSAVS
                     KKEGRAKNAIVLQHGKPMIFGENNEKGLILDGMNLKVVNLGENGVTEKDILIHDETTI
                     DFTLHLKLATMPFPEFPIALGVIRSIKAPTYDGEITVQIKDVQAKNPTRSLKDYLMKG
                     EIWEVK"
     gene            complement(4903..5898)
                     /locus_tag="PLJ82_04070"
     CDS             complement(4903..5898)
                     /locus_tag="PLJ82_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOR40715.1"
                     /translation="MVSYKELGLVNTKQMFQKAVEGGYAVPAFNFNNLEQIQAIVSAC
                     AETKSPVILQVSSGARKYANQTLLRYLAQGAVEYAKELGWENPQIALHLDHGDTFELC
                     KSCIDMGFSSVMIDGSHHSFEKNIELTKQVVEYAHAHGVTVEGELGVLAGVEDEVSSE
                     VSHYTRPEEVIEFVTRTGVDSLAISIGTSHGANKFTPAQCTKDANGVLVPPPLRFDIL
                     EEIEKQIPGFPIVLHGASSVPQEYVKTINENGGKLKDTIGIPEDQLRKAATSAVCKIN
                     IDSDGRLAMTASVRQFFNDNPEKFDPRQYLGPAREELKRLYKHKVEKVLGSAGKA"
     gene            complement(6042..7409)
                     /gene="radA"
                     /locus_tag="PLJ82_04075"
     CDS             complement(6042..7409)
                     /gene="radA"
                     /locus_tag="PLJ82_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOR40716.1"
                     /translation="MPKLKTIYVCKNCGNDSPKWFGKCSACGEWNTCVEEIEHKESKS
                     APRITGYDFQAQKPILLHEIEISQEKRIDMCNEEVNRVLGGGLVPGSMVLIGGEPGIG
                     KSTLVLQVALHLSNLRILYVSGEESAKQLKLRAERIASHQTDCYIVSETSLETIYKHI
                     QTIKPELLIIDSIQTIFTDVVDSSPGSITQVRECAASLLKFAKESNTPIFIIGHITKD
                     GVIAGPKILEHIVDTVLQFEGDQHYMYRILRSIKNRFGSTSELGIFEMRQNGLREVTN
                     PSELLLSHHQEELSGIAIASAIEGVRPFLIEVQALTSTAAYGNPQRSATGFDGRRMNM
                     LLAVLEKRAGFKIAQKDVFLNIAGGLKVKDPAIDLSVISAILSSSMDIAIQTNCCLAG
                     EVGLSGEIRPVNRIEQRIAEAQKLGFTKIILPNHNMKGLTSTFPEIELVPVKKVEDAF
                     RILFT"
     gene            complement(7486..7836)
                     /locus_tag="PLJ82_04080"
     CDS             complement(7486..7836)
                     /locus_tag="PLJ82_04080"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOR40717.1"
                     /translation="MFIQVLKSKIHRVTITEANLEYIGSITIDEDLMEAANIIESEKV
                     HIVDNNNGERFETYVIKGERGSGTICVNGAAARRVQPGDTIIIMAYAFMDFEEAKQFK
                     PTCIYPDTRTNKLI"
     gene            complement(7843..8679)
                     /gene="panC"
                     /locus_tag="PLJ82_04085"
     CDS             complement(7843..8679)
                     /gene="panC"
                     /locus_tag="PLJ82_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007666497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOR40718.1"
                     /translation="MQIITTKSELCKSLTNSTGIRIGFVPTMGALHQGHLSLVMRSKR
                     ENEVTVVSIFVNPTQFNDSRDLEKYPRTLDADINLLSQVGCDIVFAPTVDEMYPEKDT
                     RIFNFGTLETVMEGKHRPGHFNGVAQIVSKLFDAVQPHIAYFGEKDFQQLAIIREMVK
                     QLNYTITIAGCPIVRESSGLALSSRNMLLSAEEHKKAPIIYQILSESCIFVAQKQPHE
                     VRNWVLNKFQNTGLDVEYFEIVDGYSLQPVSSWNESDYIVGCIAVFCGKIRLIDNIPF
                     KR"
     gene            8946..9761
                     /locus_tag="PLJ82_04090"
     CDS             8946..9761
                     /locus_tag="PLJ82_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOR40719.1"
                     /translation="MDNVIKVLYVTQEICPYLPESEIADICRKLPQAIQEKGKEIRTF
                     MPKFGCINERRNQLHEVIRLSGMNLIIDDTDHQLIIKVASIQAARMQVYFIDNDDYFN
                     RKSTTQDENGEDFDDNEDRAIFFARGVIETVKKLRWTPDIVHCHGWMTALMPIYIKKA
                     LHDDPFFSKAKVIYSIYDNGFSKHFSPQFAQKSILEGIEKTDLKSVQNDGSFDAVTRL
                     AIDFSDGVIAASENVNTELLEYAKKSQKMTLPYQSPENYVNSYNEFYDQIVGQ"
     gene            9775..11118
                     /locus_tag="PLJ82_04095"
     CDS             9775..11118
                     /locus_tag="PLJ82_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOR40720.1"
                     /translation="MKNTFWLLIAISFLFASCKDESEDLGFLIQPSKDKITVVIDSFH
                     VATSNLFVDSIYARSSNFTLGKYTDNIYGSFNASFLTQFKYVQGATFPSNATADSLSL
                     VLYYKNFWGDSLSTHEVTVYALDELFNYEKNYYSNINVAEFTNKQTILGKKVYTAVDK
                     TIPDSIRNASDYVGSVTILLPNSLKEEFFTRSDIYTSQQAFLNFFKGIYATTTYGDGT
                     VLLIDSANIELKYSYATTTSADVDTIIKEKLIFPANKEITSVTHINHPTTLSTSITDI
                     DSIVYVHSPAGIFPKISIPFDRIIERVPSSSYNTINHIHLIAEAAILDDNSTYSEPST
                     LLLIREQDIEKFFAQSLFPATSIGIYAFLGTYNSTSKSYTFTSMADYFRMILKNEGDD
                     ANTLNDFVLVPVKTITNTRGTITSLRHLYNPRGIRLRSGKNITSPMRIEITYSQF"
     gene            complement(11170..11421)
                     /locus_tag="PLJ82_04100"
     CDS             complement(11170..11421)
                     /locus_tag="PLJ82_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOR40721.1"
                     /translation="MVKTMTFNELRKIKDSLPDGTIRKIAQQLNITEETVRNYFGGHD
                     FDKGKAVVGVHIEPGPDGGVVTLDDTTILDLAMQEMARV"
     gene            complement(11521..12249)
                     /locus_tag="PLJ82_04105"
     CDS             complement(11521..12249)
                     /locus_tag="PLJ82_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOR40722.1"
                     /translation="MNYLEIVTNIFSSIVSWDTNMLLKLNSWNNLFFDGFMWITTSIL
                     VWLPLYISVIYIIIKNKKKESIIILFSLMLAIVLADQLASSVLKPLVERLRPTHNEDL
                     MPYIHTVNGYKGGIYGFVSSHAANAFAFVVFTAFLFRQFSYISLMLLWALLLSYSRIY
                     LGVHFPLDVIVGALLGGGIGYAAFVFYQLLKRIMPQYVYKKHKTNSRHYSPFSVKDLS
                     PFFFCFVITLFFSILFAYNFSFFE"
     gene            complement(12255..13019)
                     /locus_tag="PLJ82_04110"
     CDS             complement(12255..13019)
                     /locus_tag="PLJ82_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40723.1"
                     /translation="MKYGLLIQLFLFFHISLSLNATTAQVYDIKKANDLYVATSYAQA
                     IEVYEEISKQGYESATLYYNLGNAYYRINKIAPAILNYERALRLDPSNLDIQHNLKLA
                     KAKTVDKIVTQEKNIVEIWYNSIVFATHTNVWAYMSIIFFIVALCLVLMYMFLRVSWI
                     RKITFFSSIIFFTFAICFFIFSYQQKKMVENQKEAIIFVPSSYVKSSPDQLGSDLFLL
                     HEGTKIIIKHQLGSWVQITTEDGNKGWLPVSTIQKI"
     gene            complement(13024..>14003)
                     /locus_tag="PLJ82_04115"
     CDS             complement(13024..>14003)
                     /locus_tag="PLJ82_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOR40724.1"
                     /translation="PTTSKPSSFQGAVGDLSFTSSLSKSAVKVNEAISFKLLISGNGN
                     LRLLQSPNVKFPVDWEVYEPKINDKFKVSSNGTSGTKEIEYVIIPRYVGEFDLPAVEF
                     SYFDPTRNQYNTIKTAAYNVSVEGSSTNETTSPSTTLSLSVQENVKIISDDIRFIDTQ
                     DTFVFPHKQYVFGSTLFWLAYIIPTFLFVLIIVLLHKQLKENADSSLMKIKRANKVAK
                     KRFLLAHNFLKQKNEKSFYDEIMKALWGYLSDKLAITNSDLFKDNLVSELQARGVAST
                     CIDETIAVLNDCEFARFSPSSSSELAMETMYQRACNIITSLESELKHKE"
BASE COUNT         4804 a         2462 c         2224 g         4513 t
ORIGIN      
        1 catgctcttt tttcttattc tgatataaat atggtgttac taatactagt agaattgctt
       61 tcactttgct ggggttttag gtattcaaaa aaatcaaacg taattcgcaa aattagtttt
      121 tttaaatgat taaacaaact ttttaacaat aaaaacaaca taaaaattaa gatttattta
      181 aggcaggtaa gtcattcgta tgaataaaat tagtactttt gccactcaaa cttttacaaa
      241 ctcaattcaa atgataacaa taattgatgt atttagtttt ttaggagctt ttgcgtttgc
      301 aattagcggt atccgtttgg cttcaaaaaa acaatttgat tggtttggtg cgtatattgt
      361 aggcttagta actgccattg gaggaggaac cttacgcgac ctattactag atcaacctcc
      421 tttttggatg gaacaaggat ggtacctcat cagtactctc atggctttac tttttgtggt
      481 tgcattaggc aaatatgtaa ataaattatc ttcttcgttt tttgtatttg atacaatagg
      541 cttagctgta tttacaattg ctggtatcga aaaaacatta gtatttggct attcttattg
      601 gatagctatt gtaatgggag taatgacagg atgcttaggt ggaattatta gagatatttt
      661 cataaacgaa acacccttaa ttttcagaaa agagatttat gctatcgcat ccattatagg
      721 aggtattttg tatttagtat ccctacatgt gtttaaccta agtgaattaa tctcgtacgt
      781 tataggtttt agtagcattg tcttagtgcg atttttagcc gttaaatacg aaatcagctt
      841 accccaatta cgtgattctc aataagtata tgtctactca aattaaattt tgtagtttag
      901 ctagcggaag tagtggaaac tgttattacg ttggaaacga aaaacatggc attttgattg
      961 atgctggtat tggagttaga acaataaaaa aaaggttgaa ggatattggc gttgcgttag
     1021 aatcaatact agggatattc gtaacgcacg accactttga tcatattgca tctataggtg
     1081 tactgggaga gattcaccac attcccattt tttcaacctc tgaaatatta gacggcataa
     1141 atcgttgcta caaggtaacg caaaaacttc aacatagcaa acgacccatt gcaaaaaata
     1201 aaatcattga aattggaagt ttcaccattg aggctttctc tgtatcacat gatgcctctg
     1261 actgtgtggg gtataccatt tgccatggca caaaaaaatt tacaatagct accgacttgg
     1321 ggtatatcaa tactgaagca gctcaacact taatgaatgc cacttcgttt gtggtagaat
     1381 caaattatga cgaacaaacc ctattggaaa atccatccta tccaacatat ctaaaagaac
     1441 gtattttgag caacaaagga catttgagta accacgcatt agcagaattc ttatcaatta
     1501 attaccaaga acattggcaa cacatttttt tgtgtcattt aagtaaagag aacaactcgc
     1561 ccgaatgtgc actatctacc atcgacaaaa aaattgccca tcaaagattt aatttatggg
     1621 ctaattgtac tctaatagct ttacctcgta ctacccctac tgaagttttt attatttaaa
     1681 agacggttat agttactttt cgttacatcc aagctatttt tggcgtccgt ttttgaaata
     1741 attctttgaa aaatgaattt tataccgtat ttttgtgctc ccaaaatacc atgacagttc
     1801 agataaataa aatagtacga ctacatcaat caatatcaga agtattcgta cgaatactta
     1861 acaataagta atccacgtaa aaaagaatga atactatcct atggcagaac aagtaaaaaa
     1921 agacaatcaa gacaatattg tcatccgatt ttcaggagac tctggagatg gaatgcaatt
     1981 ggcgggcaca ctttttgcca acatatcggc aatgattgga aatgatattt ctacatttcc
     2041 tgatttccct tccgagatta gagcccctca gggtacatta agcggtgtat caggctacca
     2101 agtacatata ggacccaaag tatatactcc aggagataaa actgacgtac taatagcaat
     2161 gaatcctgct gcattaaaga taaatgtaaa aaacctgaaa aatagttctg ttgtagttat
     2221 cgacactgat agttttacta aaaaagattt agaaaaagca ttattttcaa cgtctaaccc
     2281 ctttgaagaa cttggaatag gaaatatcag cgtaattgaa gcccctatag gatctatggt
     2341 aaaagaaagc cttactaatt caggattaga aggtaaagat gtgttacgat gcaaaaatat
     2401 gtttgcatta ggtttatcgt gttggttgtt tgatcgccca ttaaacctag cagagagctt
     2461 aattcgtaca aaatttgcaa aaaaaccaat tgtagccgaa gcaaacataa aggtactaca
     2521 cgatgggtat aattttggag ctaacacgca cgcttctgtg cctacttacc atattgctac
     2581 aagtacaaaa gaaaagggat tttacactga cgtagatggt aataccgcaa ctgcttatgg
     2641 tttaattgca gctgccgaaa aagcgggcat ggaattattc ttaggaagct atcctattac
     2701 acctgctacc gatattttac aagaacttgc taaacataaa gcgttgggag taaagaccat
     2761 gcaaatggaa gatgaaatag cgggtatttg ctcagctatt ggtgcaagtt ttgctggttc
     2821 gttagctgct acaaacactt ctggaccagg tttagctctt aaaagcgagg ctattggctt
     2881 agctgttatg gcagaattgc caattgtaat tatagatgtt caacggggag gaccttcaac
     2941 aggcttgcca accaaaagcg aacaaacaga cttaatgcaa gcgttatacg gtcgcaatgg
     3001 agaaagtcca gtggctgtgg tagcagcaaa atcgccaaca gactgtttcg atgctgcttt
     3061 ttgggcatcc aaaatagctg tagaacacat gaccccggta attttactca ctgatggtta
     3121 tatagcaaat ggttccggag cattcaaaat tcccgaatta gaaaactatc ctagtattca
     3181 acctcattat atcaaaaagg atcaacaaaa ctggaaacca tacacacgta acgaagagac
     3241 taaagtgcgt tattgggcaa cacctggaac agaaggtttt cagcatcgca ttggtggatt
     3301 agaaaaagat tatacaacaa gtattatttc aacagatcca gacaatcacc aacttatggt
     3361 ggaaacacgt caagaaaaaa ttaatgcaat tgcaaatttt atccctatta ttgatataca
     3421 aggagatgca aacgctgatg tattaatagt tggttggggt ggaacatacg gacatctatt
     3481 atcagctgta aatgaaatga atgagaacgg taaaaaaacg gcatttgctc attttagttt
     3541 tattagccca ttacctaaaa atacagaaga aatactgcgg aaatacaaaa aaataattgt
     3601 ttgcgaacaa aatcttggtc agtttgcagg ttatttacgc atgaaaattc atgggttacc
     3661 tatctaccaa tacaacgagg taactgggca acctttttta gtaagcgaat tagtaaagta
     3721 tttcacaaaa attatggagg aataaaaata tgtcagaatt aacatataaa ccatttcaag
     3781 catccgatta taaaagtgca caagcagtac gctggtgtcc aggttgtggc gaccattttt
     3841 tattaaattc tctacacaaa gcgatggcta acattgggat acccccgcat caaatcgcag
     3901 ttatttctgg tataggatgt tcttctcgat taccttatta tatgaatacc tatggttttc
     3961 ataccattca cggtcgtggt gctgctattg catcgggctt aaaagttgcc aatccaaaat
     4021 taagtgtatg gcaaattact ggtgatggcg attgtcttgc cattggagga aaccatttta
     4081 ttcattccat acgtagaaat atggatttaa atattgtatt agttaataat caagtatatg
     4141 gcttaacaaa aggtcagtat tcccctactt ctgatcgcgg ttacgtctgt aaatcagctc
     4201 cctatggaac agtagaagat ccttttcgtc ctgctgaatt aacatttggt gctagaggta
     4261 ctttttttgc acgtacagta gacgtagatt ctcctgtggc agtagaagta ttaacggcag
     4321 cagccaaaca caaaggagcc tcagtagtag aagtattaga aaattgcatc attttcaatg
     4381 atggaacgca cagtgctgta agtaaaaaag aaggacgtgc aaaaaatgcc attgtattac
     4441 aacatggcaa acctatgatt tttggcgaaa ataacgaaaa aggattaatc ctagatggta
     4501 tgaacctaaa agtagttaat ttaggtgaaa atggtgttac agaaaaagat attctaatac
     4561 atgatgaaac aaccatagat tttaccctac atctaaaact agcgactatg ccatttcctg
     4621 aatttccgat tgctttagga gtaattagaa gtataaaagc accaacctat gacggagaaa
     4681 taactgtgca aattaaagat gtacaagcga aaaatccaac tcgttcgttg aaagactact
     4741 taatgaaagg cgaaatttgg gaagtaaaat agtataaaaa catagccata aaaaatcccc
     4801 ctaagaaatg gacttagggg gattttttat ggctatgtta ctagtgtgat ggtaataagc
     4861 aaatcaacat acatgtattt actatcacca tagtaggaat gattatgctt ttccagcact
     4921 tcctaatact ttttccactt tgtgtttata tagtcttttt aattcttcac gagctggtcc
     4981 taaatattga cgagggtcaa atttttcagg attatcatta aaaaactgac gaacagatgc
     5041 tgtcatcgct aaacgaccat cagagtcaat attaattttg catacggctg atgttgcagc
     5101 tttacgcaat tgatcttcag gaataccgat tgtatctttt aatttaccac cattttcgtt
     5161 aatagttttc acatactctt gtggcacaga agatgcacca tgcaatacaa taggaaaacc
     5221 aggtatttgc ttttcaattt cttctaaaat atcaaaacgc aatggaggtg gcactaacac
     5281 accattagca tcttttgtac actgtgcagg tgtaaattta ttcgctccat gagaagttcc
     5341 aatagaaatc gccaacgaat caacaccagt acgtgttaca aattcaataa cttcttctgg
     5401 gcgagtataa tgactcactt ctgatgacac ctcatcttcc actcctgcta gtacacctaa
     5461 ttcaccttct acagttacac catgagcatg agcatattct acaacttgtt ttgtcaactc
     5521 aatatttttt tcaaatgaat gatgagaacc atcaatcatt actgaagaaa atcccatatc
     5581 aatacaactt ttgcataatt caaatgtatc accatgatca agatgaagtg caatttgagg
     5641 attttcccaa cccagttctt tagcatattc tacagctcct tgagctaaat aacgtaataa
     5701 agtttgattt gcatatttac gagcaccact ggatacttgt aaaataacag gcgatttagt
     5761 ttcagcacaa gccgatacaa tagcttgaat ttgctctaaa ttattaaaat tgaatgcagg
     5821 aacagcatag cccccctcca ctgctttttg gaacatttgt ttggtgttca ctaaaccaag
     5881 ttctttgtaa gaaaccattt ttttttgtgt taagtttatg aatcgtttaa ttgaattata
     5941 ttcttttgca tggcaaaatt aaaaaaaata atctacatac ttgcctaatt ctcaaaaaaa
     6001 atacacatat gctttcctta aaaaaacaaa attaaccatc cttaggtaaa taaaatacga
     6061 aaggcatctt ctactttttt cacaggcaca agttctattt ctggaaaggt tgatgtcaag
     6121 cctttcatat tatgattggg aagtataatt ttggtgaatc ctaatttttg agcctcagca
     6181 atgcgttgct caattctatt cacaggtcta atttcacccg ataagcctac ctctcccgcc
     6241 aaacaacaat ttgtttgaat agcgatatcc atacttgaag ataaaatagc actaattaca
     6301 gacaaatcaa tggcaggatc ttttactttt aatccaccag caatattgag aaagacatct
     6361 ttttgtgcaa ttttaaaccc ggctctcttt tccaatacag ccaacaacat attcatacgt
     6421 ctaccatcaa atccagtagc tgaacgttgt ggattgccat acgcagcggt actagttagt
     6481 gcttgcacct ctattagaaa tggacgaaca ccttcaatgg cagatgcaat agcaatacca
     6541 ctcaattctt cttgatgatg ggacagtaaa agttcagaag gatttgttac ttcacgcaaa
     6601 ccattctgtc tcatttcaaa tattcccaat tcagaggtac tcccaaaacg atttttaatt
     6661 gaccgcaata tcctatacat ataatgctga tctccttcaa attgtaatac tgtatctaca
     6721 atatgttcta atattttagg accagcaatt accccatcct ttgtaatatg tcctattata
     6781 aaaatgggag tattcgattc ttttgcaaat ttgagtaacg atgcagcaca ttctcttact
     6841 tgtgtaatac tacctggaga agaatcaaca acatcagtaa aaatggtttg aatagaatca
     6901 ataatcaaga gttctggttt gattgtttga atatgcttat aaatagtttc aagagatgtt
     6961 tcacttacta tataacagtc tgtttgatgt gatgcaatac gttctgctct caattttaac
     7021 tgttttgcac tttcctcccc agatacatat aatatacgta gatttgacaa atggagagca
     7081 acttgcaata ccaaggttga ttttcctatc ccaggttcac ctcctattaa taccattgaa
     7141 cctggcacta atcctcctcc taataccctg ttgacttcct cattacacat atctatgcgt
     7201 ttttcttgtg aaatttctat ttcgtgtaat aaaataggct tttgagcctg aaagtcatat
     7261 cctgttatgc gaggggctga tttactctct ttgtgttcta tttcttccac acaagtattc
     7321 cattctccac atgcagaaca cttaccaaac cactttggtg agtcgtttcc acagttttta
     7381 caaacatata tagtttttag tttaggcata catacaaatt tgttgcaaga tacaagaaaa
     7441 aatacaaata aaaaagccac tctatttaaa aagtggcttt ttgtttcaaa ttaatttatt
     7501 tgtacgagtg tctggataaa tacaagtagg tttaaactgc ttagcttctt caaaatccat
     7561 aaaggcatat gccatgataa tgattgtatc gccaggctgt actcgacgag cagcagctcc
     7621 atttacacaa attgtaccac tacctctttc gccctttatt acatatgttt caaagcgttc
     7681 tccattgtta ttatccacaa tatgaacttt ttctgactca ataatattag cagcttccat
     7741 caaatcttca tcaatagtta tactaccaat gtattcaaga tttgcctcgg taattgttac
     7801 ccgatgtatt ttagatttta aaacctgtat aaacatactt ttttaacgtt taaatggtat
     7861 attatctatt agtcttattt ttccacaaaa tactgcaata caaccaacga tataatcgga
     7921 ttcattccac gatgaaacag gttgtaagga gtaaccatct actatttcaa aatactcaac
     7981 atctaatcct gtattctgaa acttatttag tacccaattg cgaacctcgt gaggttgctt
     8041 ttgggctaca aagatacaac tttctgataa tatttggtat attataggtg cttttttgtg
     8101 ttcttcagca gaaagtagca tatttcgact actcaaagcc aaaccacttg attctctaac
     8161 aatagggcac ccagcaatcg taatagtata atttaactgt tttaccatct cacgtataat
     8221 tgctaattgc tgaaaatcct tttcaccaaa ataagcaata tgaggttgta ctgcatcaaa
     8281 taatttactt acaatctgtg ccactccatt aaaatgtcca ggtctgtgct taccctccat
     8341 aaccgtttct aatgtaccaa aattaaagat gcgagtatct ttttctggat acatttcatc
     8401 aactgtaggg gcaaacacta tatcacaccc tacttgagat aatagattaa tatcagcatc
     8461 taacgtacgc ggatactttt ctaagtctct gctatcatta aactgagtag gattaacaaa
     8521 aatacttact acagttacct cattttctct tttacttcgc attaccaacg acaaatgtcc
     8581 ctgatgtagt gctcccattg taggaacaaa accaatacga atgcctgtag aattggttaa
     8641 tgatttacac aattcagatt tggtagttat aatttgcatc atttacatca aaattgagat
     8701 gcaaaagaac aaataatatt tgggatgaaa aagaaaaacc cacataagtt atcatcaata
     8761 tataaaaaga aactttttta taccacaatc acattctgtt tgctatgata atatgattgt
     8821 ttccaatcta aaaataatac ttacaaaata gaatggattt atgggaaatt tcttgcttat
     8881 taacgatttt ttttttatat ctttgcatat tgtttttttc gaggagtatt actaattcat
     8941 ttttaatgga caacgttatt aaagttttat acgttacgca ggaaatttgc ccgtatttac
     9001 cagaatcaga gattgcagat atttgtcgta agttgcccca agcaattcag gaaaaaggca
     9061 aagaaattcg cacatttatg cccaaatttg gttgtattaa tgaaagacgc aaccaactac
     9121 atgaggtaat tcgactatca ggaatgaacc ttattattga tgacactgat catcaactca
     9181 ttatcaaagt tgcctctata caggcagcac gtatgcaagt ttattttatt gacaatgatg
     9241 attattttaa cagaaaaagt acgactcaag acgaaaacgg tgaagatttc gacgataatg
     9301 aggatcgggc tattttcttt gcaagaggtg taattgaaac agtaaagaaa ttgcgttgga
     9361 ctccagacat cgttcattgc catggatgga tgaccgcatt aatgcctatt tacattaaga
     9421 aagcattgca tgacgatcca ttcttttcta aagctaaagt catttattct atttacgaca
     9481 atggtttttc caaacatttt agtccacaat ttgcacaaaa atctatatta gagggaatag
     9541 aaaaaacaga tttaaaatcc gtacagaatg atggaagttt tgacgctgta actcgtcttg
     9601 caatagattt ttctgatggt gtaattgctg caagtgaaaa tgtgaataca gaattgcttg
     9661 aatatgctaa aaaatcacaa aaaatgacac ttccatatca atctccagaa aattacgtaa
     9721 attcgtacaa tgaattttac gaccaaattg tgggacaata aattttctca aaaaatgaaa
     9781 aatacattct ggctactaat agctatctct tttttatttg cttcatgcaa agacgaatca
     9841 gaagatttgg gttttttaat ccaaccttcg aaagacaaaa taacagttgt aatagattct
     9901 tttcatgtag caactagtaa cttatttgtt gactcaatat atgccagaag cagtaatttt
     9961 acgttaggaa aatacactga taacatatat ggcagtttta acgcttcttt tttaacacaa
    10021 tttaaatatg tgcaaggagc tacattccca tctaatgcaa cagccgactc tttaagcttg
    10081 gtactatatt ataaaaattt ttggggcgat tccctttcta ctcacgaagt aactgtatac
    10141 gcattggatg aattgtttaa ttatgaaaaa aattattatt ctaatattaa tgtagctgag
    10201 tttaccaata aacaaactat tcttgggaaa aaagtatata ccgctgtaga taaaacaatt
    10261 ccagactcca ttcgtaatgc aagtgattat gtaggaagtg taactatttt actaccaaat
    10321 tctttaaaag aggagttttt tacaagaagt gacatatata caagccaaca agctttttta
    10381 aattttttca agggaatata tgcaactaca acttatggag atggtactgt attactcatc
    10441 gattcggcta atattgaact aaaatatagt tatgcaacta ccacttctgc tgatgttgac
    10501 actattatta aagaaaaact tatttttcca gccaataagg agattacaag tgttacacac
    10561 ataaatcatc ctaccacctt atctacttca ataacagata ttgatagtat tgtatatgta
    10621 cattctcctg cgggtatttt cccaaaaatc tctatccctt ttgatagaat aattgaacga
    10681 gtgccaagtt ctagttataa taccattaat catatccatt taattgcaga agcagctata
    10741 ttagacgata atagtactta tagtgagcca tctacccttc tgctgataag agaacaagat
    10801 atagaaaaat tctttgctca aagtttattt cctgccacat ccattggcat ctatgcattc
    10861 ttaggcacct ataactccac atcaaagagt tatacattta cttcgatggc tgattacttt
    10921 agaatgatac tgaaaaatga gggggatgat gcaaatacgc taaacgactt cgtattagtg
    10981 ccagtaaaaa ccattacaaa tacaagaggt actattacaa gcttacgaca tttgtataat
    11041 cctagaggga taaggctacg cagtggcaag aatataacat ctccaatgcg tatagaaatt
    11101 acatatagcc aattttgatt acaaaaaaaa agaggaaaat tcctcttttt tttgtaatgt
    11161 cttaaaaaat taaactcgag ccatttcttg catagctaaa tctaaaatgg tagtatcatc
    11221 taatgtaact acaccaccat caggaccagg ttcaatgtgt acaccaacaa cagctttacc
    11281 tttatcgaaa tcatgccccc caaaataatt tctaactgtt tcttcagtaa tgtttaactg
    11341 ttgtgcaatt tttctgattg ttccatcagg taaactgtct ttgatttttc tcaactcgtt
    11401 gaatgtcata gtttttacca taatttcaca gtattaaatt aataaataaa taattatgtt
    11461 tttaaagttg caataaatat acgtcaatat tttatataaa aaaaatattt gacacaaaat
    11521 ttactcgaaa aaactgaaat tatatgcaaa tagtatacta aagaagaggg ttataacaaa
    11581 acagaaaaaa aatggggata aatctttaac agaaaatgga gaataatgtc tactatttgt
    11641 tttatgtttt ttatagacat actgaggcat tattcttttt aacaattgat agaatacaaa
    11701 agctgcatac ccaatgcctc ctcctaatag tgcacccaca atcacatcta aaggaaaatg
    11761 aactcctaaa taaatgcgag aataagacag caataatgcc caaagtaaca ttaaacttat
    11821 atacgaaaat tgtctaaata gaaacgctgt aaaaactaca aaagcaaatg catttgctgc
    11881 gtgtgaagat acaaatccat aaatacctcc tttatatcca tttactgtgt gaatatatgg
    11941 cattaaatcc tcattatgag taggtctcaa tctttcaaca agtggtttta aaacagatga
    12001 tgctaactga tcggctaata caatagctaa cattaaagaa aacaatataa taattgattc
    12061 tttcttttta ttttttataa ttatatatat aaccgaaata taaagaggta accagactaa
    12121 aatagaggtt gtaatccaca taaaaccgtc aaaaaaaaga ttgttccaac tatttagttt
    12181 caacaacata tttgtatccc aactaacgat agaagaaaaa atgtttgtta caatttctaa
    12241 ataattcatg tagtttatat tttttgaata gttgaaacag gcaaccatcc tttatttcca
    12301 tcttctgttg ttatttgtac ccaagagccc aattgatgtt taatgataat ttttgttcct
    12361 tcatgtaata agaataaatc actacctaat tgatcaggag aactttttac ataagaagat
    12421 ggcacaaaga taatagcttc tttttggttt tctaccattt tcttttgttg atacgaaaaa
    12481 ataaaaaagc atatcgcaaa tgtaaaaaat ataatggaag aaaaaaacgt tatttttcta
    12541 atccaactta cacgtaaaaa catatacatt aatacaagac ataatgctac aataaaaaaa
    12601 atgatactca tataagccca cacatttgta tgagtagcaa atacgattga attgtaccaa
    12661 atttcaacta tgttcttttc ttgtgtaaca attttatcta ctgttttagc ttttgcaagt
    12721 ttcaagttat gttgtatatc tagattggat ggatctaaac gcaaagctct ttcataatta
    12781 agaatggctg gggcaatttt atttatacga taataagcat ttcctaagtt ataatacaat
    12841 gtggcagatt cataaccttg cttacttatt tcttcgtata cctcaatggc ttgtgcatac
    12901 gacgtagcca catataaatc attggctttt ttaatatcat atacctgagc agtagtagca
    12961 tttagggaca atgatatatg aaaaaaaaga aacaattgga ttaacaaacc gtatttcata
    13021 tcattattct ttatgtttta gttcactttc aagacttgta ataatattac atgctctttg
    13081 gtacattgtt tccatagcaa gctcagagct cgaagaagga gaaaaacgtg caaattcaca
    13141 atcatttaag accgcaatag tttcgtcaat acatgtacta gcaactcctc tcgcttgtaa
    13201 ttctgaaacc aaattatctt taaacaaatc agaattagta attgccaatt tatcagatag
    13261 atacccccat aaagctttca ttatttcatc gtagaatgac ttttcattct tttgtttaag
    13321 aaaattatgg gctaataaaa acctcttttt agctacttta tttgctcgtt ttatcttcat
    13381 caatgagcta tctgcatttt cttttaattg tttatgtaac aaaacaataa ttaacacaaa
    13441 taagaatgtt ggaatgatat acgccaacca aaataaagtg gaaccaaata catattgttt
    13501 gtgtggaaac acaaacgtat cttgtgtatc aataaatcgg atatcatcac taataatctt
    13561 aacattttct tgcactgaca atgacaaggt agtagaaggc gatgtagttt catttgttga
    13621 ggagccttca acactaacat tataagccgc tgttttgatt gtgttgtatt gatttcttgt
    13681 aggatcaaaa tatgaaaatt ctactgcggg caaatcaaat tctcctacat aacgaggaat
    13741 aattacatac tcaatttctt tcgttccact agttccatta gagcttactt taaatttatc
    13801 atttattttt ggttcataaa cttcccaatc tacaggaaat ttaacatttg gagattgtaa
    13861 taatcttaaa ttaccattcc ctgatatgag taatttaaag ctaatggctt catttacctt
    13921 aacagcagat ttagacaaac tagaggtaaa agataaatcg ccaacagcac cttgaaaaga
    13981 agatggtttg cttgttgtag gca
//
LOCUS       DAOSRP010000045        13983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4444_length_13983_cov_4.861646, whole genome shotgun sequence.
ACCESSION   DAOSRP010000045 DAOSRP010000000
VERSION     DAOSRP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_13983_cov_4.861646"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLJ82_04120"
     CDS             <1..509
                     /locus_tag="PLJ82_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOR40725.1"
                     /translation="AGTILENIQSGNIKKMVVMSGCDARQKSRQYYTDFAEALPSDTL
                     ILTSGCAKYRYNKLPLGDINGIPRVLDAGQCNDSYSWAIVALKLKEVLGLEDINDLPL
                     VFNIAWYEQKAIIVHLALLYLGVKDTHIGPTLPAFMTPNVLKLITEKFGVKTNATVEE
                     DMELFGLT"
     gene            641..1630
                     /locus_tag="PLJ82_04125"
     CDS             641..1630
                     /locus_tag="PLJ82_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40726.1"
                     /translation="MINKHHFLTYDTNWLTRFYHQTSKRATMLRNKELQLTAPYEIIV
                     DYRNQGQLDWGSWEYSGEFCIAKELTSIKKQFISMLEQNDGLYKGNNVCPRVKTYSIM
                     QNKLLLKLEKASYFDQVATNLALDNPLDETDATSLQARCLREWDIQQSNTPHGTLPSF
                     ESSQLANTIGVSLGITVKNKNNEKVFLTRTRTSHVAVAAHQEVLPFSFSLNFNPSPSM
                     FGKQQSIKELIKADFIEEQAEELGIDASVLDFENVKPLLLCRELCRGGKPQFFCEIEL
                     DMCYEDLQNHISENLLPQKEFTSTIKSYTLNEAQQSFSTLSPEIQAFVVAKSS"
     gene            complement(1642..2676)
                     /gene="galE"
                     /locus_tag="PLJ82_04130"
     CDS             complement(1642..2676)
                     /gene="galE"
                     /locus_tag="PLJ82_04130"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOR40727.1"
                     /translation="MTKIIVTGGTGYIGSHTVVELQQAGYEVVIIDNLSNSSVDVLQG
                     IFAITGVMPSFEKIDCTDYQAMESVFKKHADAQAIIHFAASKAVNESVEKPLLYYRNN
                     LVSLMNLLELMATYTMSNLVFSSSCTVYGQPDILPVTENSPIKVSLSPYGNTKQIGEE
                     IITDTVHANKSIHAIILRYFNPVGAHESALIGELPNGVPQNLMPFVTQTAIGIREKLS
                     VFGNDYDTPDGSCIRDYIHVVDLAKAHVVAVERMLQGKQKQNPETFNVGTGVGLSVLE
                     IVQKFQDVNNVSLNYQIVGRRAGDIEKVWADATIANNELGWKATKSVEEIVQSAWKWE
                     LQLQKKRKAN"
     gene            2824..3249
                     /gene="ruvX"
                     /locus_tag="PLJ82_04135"
     CDS             2824..3249
                     /gene="ruvX"
                     /locus_tag="PLJ82_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOR40728.1"
                     /translation="MGRIIAIDYGKKRVGIAVTDTLQLIANGLTTVASHEVIAFLERY
                     IQTEPVDKIIIGLPKQMNNELSESMKYITPFVHTLKKRITHIPIEYVDERFTSVLAHR
                     TMLEAGLKKKDRQNKALVDTISATIILQTYLESIKTNTL"
     gene            3246..3797
                     /gene="def"
                     /locus_tag="PLJ82_04140"
     CDS             3246..3797
                     /gene="def"
                     /locus_tag="PLJ82_04140"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOR40729.1"
                     /translation="MIRPIVVYGNAVLRKKADPIDKNYPELSSLINDMFDTLSHAEGV
                     GLAAPQVGLPIRLFIVDLSILGDDEPSFVNFKKVMINPLITSFEGEELSQEEGCLSIP
                     GISETVKRSDKISIEYYDENWQKHVEQYSGFPSRVMQHEYDHIEGHVFVDKISPIRRQ
                     MISSKLNALTKGKFNCHYKVQKV"
     gene            complement(3794..4375)
                     /gene="coaE"
                     /locus_tag="PLJ82_04145"
     CDS             complement(3794..4375)
                     /gene="coaE"
                     /locus_tag="PLJ82_04145"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOR40730.1"
                     /translation="MLKVGITGGIGSGKTHVCQLLETVGIPIFYSDIVGKEITNTNPK
                     VRAAIIELLGEEAYKNDVLNAKFVASKVFDDKSLLEKINAIIHPVVFAAFEDWSMQFE
                     GHKIVALESAVLFESGFDKYIDKSIVVTAPLQVRINRIMKRDKLTYPEVEARIKNQLS
                     DEEREARADFVIKNDGVEHLPSQLFAFLKAVDF"
     gene            complement(4382..5314)
                     /locus_tag="PLJ82_04150"
     CDS             complement(4382..5314)
                     /locus_tag="PLJ82_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOR40731.1"
                     /translation="MNKNAFIFLFFCIAATIFWFVVVLDKEYETRIAIPIQYINVPVD
                     IEIVHDMPSHLDVRIRDKGISLLVYRQKNYFDPLVFDFNKHTFIRNALSVSSSALFEK
                     VISEQLLSTSVIKDYSPRTFDFQHIKLNKKRVPVRLRYDFSCRKQYSLAGDLIVSPNE
                     VTIYGNKERIDTIHYVTTERVVLTDLTDTTKITVLVEKLPHTRFLPNNVSVFIPVEVF
                     TESSMHIPITVHNVPDNVAVRVIPADVEVLYQVGKSMYNKVNPMDFVISVDYNNVVRS
                     TSRRQILTLEKYPTYLRNVKLGTMEIDCFIEIKD"
     gene            complement(5402..5677)
                     /gene="yajC"
                     /locus_tag="PLJ82_04155"
     CDS             complement(5402..5677)
                     /gene="yajC"
                     /locus_tag="PLJ82_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOR40732.1"
                     /translation="MGQYGSLIMMALIFVVFYFFMIRPQMKKQKEIKKFRDSIAIGDK
                     VITSGGIYGKVKDIKQDSFVLEIAENVRVTVDKNSVFATAADAPTTR"
     gene            complement(5763..6713)
                     /gene="nusB"
                     /locus_tag="PLJ82_04160"
     CDS             complement(5763..6713)
                     /gene="nusB"
                     /locus_tag="PLJ82_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR40733.1"
                     /translation="MINRTLIRIKTVQLMYAFLNNKEKSLQATEKELLFSLEKTYELY
                     NLLLLLSVEITSFADKKIEMGKKKLRPTPEELNPNMRFVANSFVAQLSSNSALQAYVA
                     DKKISWIEHADLVKKLYERIVDSSIYQEYMTAKESGYEADKQVWRKIYRKIFLVDEEL
                     NDRLEDMSIFWNDDIEIVVSFVEKTIKRFEQANGIQQEILPMYKEEDDKEFALKLIRN
                     AVLEDDTFTQLIDEHTKNWEIDRIAFIDKVIMKVALAEITDFPTIPVSVTLNEYIEIA
                     KVYSTDKSSIFVNGVLDTIVNTLRNTNKLVKVGYFLKDTK"
     gene            6901..7257
                     /locus_tag="PLJ82_04165"
     CDS             6901..7257
                     /locus_tag="PLJ82_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOR40734.1"
                     /translation="MLFFKKSAPTPTETKETETSGVKEKEILYSKAVKAGKRIYYLDV
                     KKDKKDDLFIAITESKKKPTGTDGQFAFEKHKIFLYKEDFEKFEEALTEVLTYIRTTP
                     HNELESESEKENESAG"
     gene            complement(7343..7510)
                     /locus_tag="PLJ82_04170"
     CDS             complement(7343..7510)
                     /locus_tag="PLJ82_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR40735.1"
                     /translation="MAHLITEDCIACGTCISECPVEAISEGDIYVIDADVCTDCGTCV
                     DVCPTEAIKPA"
     gene            7720..8991
                     /locus_tag="PLJ82_04175"
     CDS             7720..8991
                     /locus_tag="PLJ82_04175"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOR40736.1"
                     /translation="MKVDVLLGLQWGDEGKGKVIDVLTPNYDVIARFQGGPNAGHTLE
                     FDGKKFVLHTIPSGIFQGDKINIIGNGVVIDPIVLKNEIEKLEKAGYDLSQKLVISKK
                     AHLILPTHRLIDAAQEAQKGDAKIGSTLKGIGPTYADKISRTGLRVGDLLHNFTEKYA
                     KKIADHTRLLDQMGFSYSLDGLEDEWMEATKTLLRFQLIDSEHVVNELIADNKRILAE
                     GAQGTMLDIEFGSYPFVTSSNTVSAGVCTGLGVPPTQIGEVYGIMKAYCTRVGSGPFP
                     TELFNEEGEEMRKKGNEFGSTTGRARRCGWIDLVALKYAIMINGVTQLIMMKSDVLDQ
                     FDVIKACVGYKIDGEEYDYFPYEINTNLEPMYVELPGWKTDMSKMQKESQFPQAFNDY
                     LDFLEDELEVPIRIVSVGPDREQTILRYINE"
     gene            9076..9753
                     /locus_tag="PLJ82_04180"
     CDS             9076..9753
                     /locus_tag="PLJ82_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOR40737.1"
                     /translation="MIIYVIFGVVALSSWLIQYRLKSKFEKYSKLALGNGMSGKEVAE
                     KMLRDNGISDVTVISTSGHLTDHYNPTNKTVNLSEAVYNSASVAAAAVAAHECGHAIQ
                     HARSYAFLQMRSKLVPVVSFASNWVQWVLLAGILLMQVMPEIMLAGIVLFAFTTLFSF
                     ITLPVEINASSRALAWLSNAGITNAFNHNEAKDALKWAAYTYVVAALGSLATLVYYIF
                     IFLGRRN"
     gene            9836..10639
                     /locus_tag="PLJ82_04185"
     CDS             9836..10639
                     /locus_tag="PLJ82_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR40738.1"
                     /translation="MNKYKVSAFVRKIGFIQLLDKIRFYVDYIRTYNKRRNFIKNNPN
                     VILPPAYYLYETYNLNYASYYYNSMDTAKWIINLIEKYQSTTNIKVLDWGCGPGRVIR
                     HIASMLDKSCELHGSDYNSRYVAWCKRHIPEVSFQQNQLAPPMVYENNYFDVVYGISI
                     FTHLSKEMHYKWFNELMRILKPGGMVILTLHGEAFKLQLSEEEQQKFDENQLIIKSHT
                     KEGHRTYGAFHPTQFVHELIGKHELVEHVKGNVTDGKPEQDIWVIRKRG"
     gene            complement(10664..12052)
                     /gene="mnmE"
                     /locus_tag="PLJ82_04190"
     CDS             complement(10664..12052)
                     /gene="mnmE"
                     /locus_tag="PLJ82_04190"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOR40739.1"
                     /translation="MNPTICAISSGQHQAAIALIRISGEEALRMCDTFFVAANKAVTL
                     STMQPNSIKFGRIFQQNELIDEVLVSVFKAPHSFTGEDMVEIACHGSSFIQQQILQLL
                     IQQGCQLAQPGEFTRRAFLNGKMDLSQAEAVADLIAAQSASSHRVAMNQMRGGFSKEL
                     AVLRDKLLEFTALIELELDFSEEDVEFADRSSLMALATIIQTKLASLARSFEVGNVIK
                     NGIPVAIVGETNAGKSTLLNLLLNEEKAIVSDIHGTTRDVIEDIVSIQGITYRFIDTA
                     GIRETQDAIEKMGIKRTYQQIEQASIILWVIDSTQVNEHIDWMAERIVSRSEGKKLIL
                     VFNKLDKINEEECRVLELLFENITAERLFISAAKHQHIDALLHHLHQAAAIPSIGDQD
                     VIITNMRHFQALTQALSAIERVQTGLSTGISGDFVSQDIRECLHYLGEITGQISNDEV
                     LGAIFSRFCIGK"
     gene            complement(12100..12228)
                     /locus_tag="PLJ82_04195"
     CDS             complement(12100..12228)
                     /locus_tag="PLJ82_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40740.1"
                     /translation="MKKIITSIAIAIILLLSVSSCQTKHCLSQVKQVQVETPTINS"
     gene            12378..12794
                     /gene="mscL"
                     /locus_tag="PLJ82_04200"
     CDS             12378..12794
                     /gene="mscL"
                     /locus_tag="PLJ82_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOR40741.1"
                     /translation="MGLLKEFKAFAMKGNVIDLAVAVIIGGAFGKIVSSLVADVIMPP
                     IGLLVGGVNFTELSIVLKEATETTAAVTLNYGNFLQATFDFIIIAFAIFMFIKGLSKL
                     EKKKEEPVVPAAPVEPTPSKEEILLAEIRDLLKEKK"
     gene            complement(12851..12988)
                     /locus_tag="PLJ82_04205"
     CDS             complement(12851..12988)
                     /locus_tag="PLJ82_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40742.1"
                     /translation="MKKNLIKVVCVCAFLAMLFNSCKTPCHCGLGQVEADKATVETVQ
                     S"
     gene            complement(13011..13490)
                     /gene="ispF"
                     /locus_tag="PLJ82_04210"
     CDS             complement(13011..13490)
                     /gene="ispF"
                     /locus_tag="PLJ82_04210"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOR40743.1"
                     /translation="MKIRVGFGYDVHQFAPNRALWLGGVSIEHSLGLLGHSDADVLIH
                     AICDALLGAANLRDIGYHFSDTSPEFKNIDSKILLAKTMNLLREQGYELSNIDATVCA
                     EEPKINPHIANMKETLAQVMHVSEEDISIKATTSEKMGFVGRKEGMAAYATVLIYKQ"
     gene            complement(13517..>13983)
                     /locus_tag="PLJ82_04215"
     CDS             complement(13517..>13983)
                     /locus_tag="PLJ82_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018919912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOR40744.1"
                     /translation="NKLSVNLDLNKLLVPSRPVAPTQGDDESDADYALRESKYETSLS
                     DYNNGDPIAGIFTSFSDAPGGFSEELQEIMWSLGLEYAYNNQFFARAGYFNESANKGN
                     RKYATIGAGFKLNVFSLDVAYVVSVAQNNPLDQTLRFSLAFTLDGIKQLTAR"
BASE COUNT         4673 a         2585 c         2339 g         4386 t
ORIGIN      
        1 tagcaggcac cattttagaa aacattcaat cgggcaacat caaaaaaatg gttgtcatgt
       61 cgggttgcga tgcacgtcaa aaatcacgcc aatactacac agattttgcc gaagccctgc
      121 catcagacac cttaatatta acctctggct gtgccaaata ccgctacaac aaactacctt
      181 taggagatat caacggaatt cctcgcgtat tggatgctgg tcagtgcaac gactcctact
      241 catgggcaat cgtagcttta aaactaaaag aagtgcttgg tttagaagat attaatgatt
      301 taccattggt gtttaatatt gcttggtacg agcaaaaagc cattattgta caccttgctt
      361 tgttatatct aggagtaaaa gacacacata tcggaccaac tctccccgcc tttatgactc
      421 cgaatgtatt aaaacttata acagaaaaat ttggagtaaa aacaaatgca accgtagaag
      481 aagacatgga actatttggt ttaacataaa cgactacgaa aagcagtagt ttgtaataaa
      541 aaatacgtgc tccatcttca ttcggagaat aaatagtacc tttgtataga cctaacatgt
      601 atgtaaatat gttaggtcta atgtatttag gacttttatt atgatcaaca agcatcattt
      661 tttaacatac gatacaaatt ggcttactcg cttttatcac caaacgagta aacgtgcaac
      721 tatgttgaga aacaaagaac tacaactaac agcaccatac gaaattatag ttgattacag
      781 aaatcaaggt caactagatt ggggtagttg ggaatatagt ggtgaatttt gtattgcaaa
      841 agaattaaca agtataaaaa aacagttcat cagcatgctg gaacaaaacg atggtttata
      901 caaaggtaac aatgtgtgtc cacgcgtcaa aacgtattcc attatgcaaa ataaactgtt
      961 gttgaaactt gaaaaggcaa gttattttga tcaagtggca acgaatttag ctttagataa
     1021 tccactcgac gaaacagatg ccacatcatt gcaagcacgc tgtctaagag aatgggacat
     1081 acagcaatca aatacaccgc acggaacgct tccttctttt gagtcctccc aattagccaa
     1141 tacaattggc gtatcacttg gaattactgt aaaaaacaaa aataatgaga aagtgttttt
     1201 aacccgcaca cgcacatcac atgttgccgt agctgctcat caagaagttt tgccattttc
     1261 attttccctc aatttcaacc cttcaccatc tatgtttgga aaacagcaat caataaaaga
     1321 actgattaaa gcagacttta tagaagaaca agcagaagaa ttgggtattg atgcctctgt
     1381 attggatttt gagaatgtaa aacctttgtt attgtgcaga gaattgtgca gagggggaaa
     1441 acctcaattc ttttgcgaaa ttgaacttga tatgtgttac gaagatttac aaaatcacat
     1501 ctcagaaaac ctcttgccac aaaaagaatt tacctccaca ataaagtcat atacactgaa
     1561 tgaagcacaa cagtctttct caaccttatc gcccgaaatt caggcatttg tagtagcaaa
     1621 aagctcctaa cataggaatt gctagttggc ttttcttttc ttctgcaact gaagctccca
     1681 cttccaagcc gattgtacaa tctcttctac cgacttcgtt gctttccatc ctagctcatt
     1741 attggcaata gtagcatcag cccatacttt ttcaatatcg ccggcacgcc ttcctacaat
     1801 ttggtagttg agcgaaacat tgtttacatc ctgaaacttt tgtacaattt caagcacaga
     1861 caatcctaca cctgtaccca cattaaatgt ttctggattt tgtttctgtt tgccttgcaa
     1921 catacgctcc acagctacca catgtgcctt tgccaaatct accacatgta tataatcgcg
     1981 aatacaagat ccgtcaggag tatcgtagtc attgccaaac acacttaatt tttctctaat
     2041 accaatagct gtttgcgtta caaaaggcat taaattttgt ggcacaccgt tgggcaattc
     2101 tccaatcaag gcactttcgt gtgctcctac tggattaaaa taacgtaaaa taatggcatg
     2161 aatgcttttg tttgcatgta ctgtatcggt aataatctcc tcaccaattt gtttcgtgtt
     2221 gccataagga gaaagggata cttttattgg agaattttca gttacaggca atatatctgg
     2281 ttgaccatat acggtgcacg atgacgaaaa caccaaattt gacatagtat atgtagccat
     2341 taattctaat agattcatca acgacactaa attattgcgg tagtacaaca aaggcttttc
     2401 caccgattca ttcaccgcct tactggcagc aaaatgaata attgcttgtg catctgcgtg
     2461 ttttttgaat acagactcca ttgcttgata atcagtacaa tcaatctttt caaaacttgg
     2521 cataacgccc gtgatagcaa aaataccttg caacacatct acagaagaat tagataagtt
     2581 gtcaatgatg acaacttcat aaccagcttg ttgtaactca accactgtat gcgaacctat
     2641 ataacccgtg cctcctgtaa caataatttt tgtcatacaa ttaaattttt gcacaaagat
     2701 acatattttt cagatacaga gtagaaagtg cccatgctaa gggttaaaaa agtgtctaaa
     2761 tttttaaaaa atactagtaa atttgctttt atggcggttt atagctactt ttggtgctta
     2821 attatgggaa gaattattgc tattgattat ggcaaaaaac gtgtagggat agctgttacc
     2881 gacactttac aactgattgc caacggatta acaacggtgg caagtcacga agttattgct
     2941 tttttagaac gctatataca aacagaaccc gtggataaaa tcattatagg attacccaaa
     3001 caaatgaata atgagctatc tgaatcgatg aaatacatca caccgtttgt acatacgctc
     3061 aaaaagagaa tcacgcatat tcccattgaa tacgtagatg agcgatttac atctgtgcta
     3121 gctcatcgta ccatgctcga ggctggatta aaaaagaaag atagacaaaa caaagctttg
     3181 gtggatacca ttagtgcaac catcatttta caaacatacc ttgaaagtat aaaaactaac
     3241 acattatgat acgtcctata gttgtttatg gcaatgctgt attacgcaaa aaagctgacc
     3301 caattgacaa aaactaccca gaactcagtt cattaatcaa cgacatgttt gatacccttt
     3361 ctcatgcaga gggagtagga ttagccgcac cacaagtggg actccctatt cgtttattta
     3421 ttgttgattt gtcaatttta ggtgatgacg aaccaagttt tgtaaatttc aagaaagtaa
     3481 tgattaatcc tttaattaca tcttttgaag gagaagaatt atctcaagaa gaaggatgct
     3541 taagcatacc tggtatttct gaaacagtaa aaagaagcga caaaatttca attgaatact
     3601 atgacgaaaa ttggcaaaaa cacgtcgagc aatacagtgg tttccctagc agagtaatgc
     3661 aacacgaata cgaccatatc gaagggcatg tatttgtaga taagatatca cctatacgca
     3721 ggcaaatgat ttcgtcaaaa ctgaacgcat taacgaaagg taaatttaat tgccattaca
     3781 aagtgcagaa agtttaaaaa tcaactgcct tcaaaaaagc aaataattga gaaggcaagt
     3841 gctccacccc atcgtttttg ataacaaaat cggctcttgc ttctctttct tcatcagaaa
     3901 gttgattttt aattcttgct tctacctctg gataagtaag tttatctctt ttcataattc
     3961 tattaatgcg tacctgtaat ggtgctgtta ccacaataga cttatcaata tacttgtcaa
     4021 aaccactttc aaacaataca gcagattcta atgcgactat tttgtgccct tcaaactgca
     4081 tgctccaatc ttcaaaagct gcaaatacta ctggatgaat aattgcattt attttctcta
     4141 acaaagattt atcgtcaaac actttactcg caacaaattt agcattcaac acatcattct
     4201 tatatgcctc ttcacccaat aactctataa tcgcagcacg aactttagga tttgtattgg
     4261 ttatttcttt gccaacaata tcagaataaa aaattggtat accaactgtt tctagtaatt
     4321 gacatacatg tgttttacca ctaccaattc ctcctgttat acctactttc aacatacaca
     4381 attaatcttt tatttcaata aaacaatcaa tttccatagt acctaacttc acatttcgta
     4441 ggtaagttgg gtatttctct agcgtaagaa tttgtcttct tgacgtactt cgcaccacat
     4501 tattataatc cacactaata acaaagtcca ttggatttac cttattgtac atacttttgc
     4561 ctacttgata cagaacctca acatctgcag gtattactct aacagcaaca ttatctggta
     4621 cattatgcac tgtaatagga atatgcatcg atgattctgt aaacacttcc actggaataa
     4681 atacagaaac attattgggc aagaaacgtg tgtgtggtaa tttctctacc aaaacagtaa
     4741 tttttgtggt atcagttaag tccgtcaata ctaccctttc agtagtaaca tagtggatag
     4801 tatcaattct ttctttgttt ccatatatag ttacttcatt aggagacaca atcaaatctc
     4861 ctgcaagact atattgcttt cggcaactaa aatcatatct caatctaaca ggaacacgtt
     4921 ttttattcaa ttttatgtgc tgaaaatcaa atgttctcgg agaataatct ttaatcaccg
     4981 atgtggataa tagttgttca ctaattactt tttcgaacaa tgcagaagaa gatacactta
     5041 aagcattacg aataaaagtg tgtttattaa aatcaaaaac taaagggtca aaataatttt
     5101 tctggcgata cactaacaaa ctaatcccct tatcacgtat ccttacatct aaatgagaag
     5161 gcatatcatg tactatttca atatctacag gaacatttat gtattgaata ggaatagcaa
     5221 tacgagtttc atattcttta tcaagcacta ctacaaacca aaaaatagta gctgctatac
     5281 aaaaaaacaa aaagatgaag gcattcttat tcaacaagag agcctttatc tttccgttta
     5341 tacttataaa aaaattatat agcctattca catacatgtt cggcacttaa aaaaatacaa
     5401 attaacgagt agtaggagca tctgcagcag tagcaaacac agaattttta tctactgtta
     5461 cacgtacatt ctcagctatt tctagtacaa aagaatcttg tttgatatct tttaccttgc
     5521 cataaatacc acctgaagtg attactttat ctccaatggc aatgctatca cgaaattttt
     5581 ttatttcttt ttgttttttc atttgtggtc taatcataaa gaaataaaaa accacaaaaa
     5641 taagagccat cataatcaat gatccatatt gacccattgg accaccttct tgtgctgttg
     5701 cttgtaataa aatgcttaac atattctcag tatttaaatt aatgttgaca aaattacgtt
     5761 atttatttgg tatcttttaa aaaatatccg acttttacta atttatttgt atttcttaac
     5821 gtgtttacaa tagtatctaa tactccattc acaaaaatgc tacttttatc ggtactatac
     5881 actttggcta tttcaatata ttcgttcaac gttacactaa ctggaattgt cgggaaatcg
     5941 gttatttcgg ctaaagccac tttcataatt actttatcaa taaaagcaat tctatctatt
     6001 tcccaatttt tagtatgttc atctatcaac tgcgtaaagg tatcatcctc taatactgca
     6061 tttcgaatca attttaaagc aaattcttta tcatcttctt ctttatacat gggtaatatt
     6121 tcttgctgta taccattagc ttgttcaaaa cgtttgatgg ttttttcaac aaaacttaca
     6181 acaatttcaa tatcatcatt ccaaaaaata ctcatatcct ccaacctatc attcaattct
     6241 tcatccacta aaaatatttt acgataaatt ttccgccaca cttgtttatc agcttcgtat
     6301 ccactttctt ttgccgtcat atattcttga taaatagaag aatcaacaat acgttcatac
     6361 aactttttca ctaagtcagc atgttctatc caagaaatct ttttatctgc cacatatgct
     6421 tgcaaagcac tatttgatga caattgtgct acaaacgagt ttgctacaaa gcgcatatta
     6481 ggattcagtt cttcgggagt agggcgtaat tttttcttac ccatttctat ctttttatca
     6541 gcgaacgatg taatttcgac agataaaaga agaagcaaat tatagagttc ataggtcttt
     6601 tctaaactaa aaagcaattc tttttcggta gcttgaagag atttctcctt attgtttaaa
     6661 aaagcgtaca tcagctgtac agttttgata cgaattaaag ttctatttat catctttatg
     6721 atataatttt atgacacaaa gatacgctaa tatttctaaa ggtttacttt tctagtttaa
     6781 aaaaaatgtt attttgacac tttcgaggtt ttttttctgt aatgtttgtt ttttagcgaa
     6841 attttcatca tttttgtaac gtattgataa atcatttgta ataagattaa attatatagt
     6901 atgttatttt tcaaaaaatc agcacctaca cctaccgaaa ccaaggaaac agaaacttcc
     6961 ggcgtgaaag aaaaggaaat tttatactcc aaagcagtaa aagcagggaa acgcatctat
     7021 tatttagatg taaaaaaaga taaaaaagat gatttattta tcgctataac agaaagtaaa
     7081 aaaaagccta caggaacaga tgggcagttt gcttttgaaa aacacaaaat ctttttgtac
     7141 aaagaggatt ttgaaaaatt tgaagaagca ctaacagaag tattaactta tattcgcact
     7201 actccacaca atgaacttga atcggaaagc gaaaaagaaa atgaaagtgc aggataatca
     7261 atgaaatatg tttagtatat aaaaaaagca atatcacatg tgatattgct tttttttgtt
     7321 tgtaatgtag attgtaatat acttaagctg gtttgattgc ttctgtagga catacatcca
     7381 cacatgttcc acaatctgta catacatcag catcaataac ataaatatcg ccttcagaga
     7441 tagcctctac aggacattcg ctaatacatg taccacaagc gatacaatct tctgtaatta
     7501 agtgagccat tttttcttta tttaaaagtt aacgtgttgc aaaagtacat tattttttta
     7561 taacacaaaa attactatcc attttttgaa aaaaaaagac aactctaccg ttgaaaagtt
     7621 aaaatataat ggtatataat aaaaaaatag tatctttacg ggttcaaaaa aataagaaca
     7681 taatttttat acgcaaaaac aatattgaag taaaaaagaa tgaaagtaga tgttttatta
     7741 ggcttgcaat ggggagatga aggcaaaggg aaagtgattg atgtattaac ccctaattat
     7801 gatgtaattg cccgatttca aggaggacca aatgcgggac atacattaga atttgatggt
     7861 aagaaattcg tattacatac tatcccctct ggtatttttc aaggagataa aataaatatt
     7921 attggaaatg gagttgttat tgatcccatt gtcttgaaaa atgaaataga gaaactagaa
     7981 aaagctggat atgatttatc ccaaaaatta gttatttcta aaaaagcaca cctcatttta
     8041 cctactcacc gcttaataga cgccgcacaa gaagcacaaa agggtgatgc aaaaattggt
     8101 tctacgctca aaggaattgg acctacttat gctgacaaaa ttagccgaac cggtcttcgg
     8161 gtgggagatt tactacataa ttttaccgaa aaatatgcaa aaaaaatagc tgaccatacg
     8221 cgtttgctcg accaaatggg gttctcttat tcgttagatg ggctagagga cgaatggatg
     8281 gaggcaacaa aaaccctcct tcgttttcag ttaattgata gcgaacatgt tgtaaacgaa
     8341 ttaatcgctg ataataaacg tattttagct gaaggtgcac aaggcacgat gcttgatatc
     8401 gaatttggtt cgtatccttt tgtaacatca tcaaacactg tatcagctgg agtttgtaca
     8461 ggactaggcg ttcctcctac ccaaattgga gaagtatatg gcattatgaa agcatattgt
     8521 acgcgcgtgg gcagtggacc tttccctacg gaattgttta acgaagaggg agaagaaatg
     8581 cgtaaaaaag ggaatgagtt tggttcaact acgggcagag ctcgccgttg tggctggata
     8641 gatttagtag cactgaaata cgctattatg attaatggtg taacgcaatt aattatgatg
     8701 aaaagcgatg tgctcgacca atttgacgta ataaaagcat gtgtaggcta taaaattgat
     8761 ggcgaagaat atgattattt cccttacgaa ataaacacca acttagaacc tatgtatgta
     8821 gaattgccag gatggaaaac ggatatgtcg aaaatgcaga aagagagtca atttccacaa
     8881 gcgtttaatg attatctcga tttcttagaa gacgaattgg aagtacccat tcgcattgta
     8941 tcggtaggac ccgatagaga acaaacaatt ttacgttaca ttaacgaata acggggtgtt
     9001 tttaacactg acaaacgcaa aattggcagt atttttgatg tatgtaattg tgtataacca
     9061 ttaacgtaat tttgtatgat aatttatgta atttttggag tagtagccct gagtagttgg
     9121 cttatccaat accgtttgaa atcaaagttt gaaaaatact ctaaacttgc tttaggcaat
     9181 ggtatgagtg gaaaagaagt agcagaaaaa atgcttcggg ataatggcat cagcgatgta
     9241 acagttattt ctacaagcgg acatttaaca gaccattaca accccactaa taagacagtc
     9301 aacttaagtg aagcagtata caactctgcc agtgtggcag cagcagcagt agccgctcac
     9361 gaatgcggac acgccataca acacgctcgc tcgtatgcgt ttttgcaaat gcgttctaaa
     9421 ttagtgccag tagttagttt tgcttctaac tgggtacaat gggttctttt agcaggtatt
     9481 ctacttatgc aagttatgcc agagattatg ttagctggta ttgtattatt tgcttttact
     9541 accttattta gttttattac tctacctgta gaaattaatg caagcagcag agccttagct
     9601 tggttgtcaa acgcaggtat taccaatgct tttaaccaca acgaagccaa agatgcactt
     9661 aaatgggctg catatacata tgtagtagct gccttgggtt cgttagcaac cttggtatat
     9721 tacatcttta tatttttggg cagacgcaat taagctaaaa agcaatgaac aagaaaaggg
     9781 gtagataatt caatcatccc cttttttata tacaacaaac aaatttcaca cattaatgaa
     9841 taaatataaa gtttctgctt ttgtccgcaa aattggcttt atccaactgt tggataaaat
     9901 tcgtttttat gttgattata ttcgaacata caataaaaga agaaatttta taaaaaataa
     9961 tccaaacgta attttgcctc ccgcctatta tttatacgaa acgtacaact tgaattatgc
    10021 ctcgtattat tacaatagta tggatacggc aaaatggatt attaacctta tagagaaata
    10081 ccaatcaaca acgaatatca aggtattgga ttggggatgt ggtccaggca gggttatacg
    10141 acacattgct agcatgcttg acaagagttg cgaactacat ggctctgatt acaacagccg
    10201 atatgtagcg tggtgtaaac ggcatatacc agaagtaagc tttcaacaaa accaattagc
    10261 tccacccatg gtatatgaaa acaattactt tgatgtagta tatggtatat ctatttttac
    10321 acacttatcc aaagagatgc actacaaatg gtttaacgaa ttaatgcgta ttctcaagcc
    10381 tggaggcatg gttatactta ccttacacgg tgaagcgttt aaactgcaat tatcagaaga
    10441 agaacagcaa aaatttgacg aaaaccagct tatcatcaaa tcacacacca aagaaggaca
    10501 tcgcacgtat ggcgcctttc atccaactca atttgtacac gaactaatcg gaaaacacga
    10561 actagtagag catgtaaaag gaaatgtaac agatggaaaa cctgaacaag atatttgggt
    10621 tatacgcaag agaggatgag ggggggggaa taaaccagtt tggttacttc ccgatacaaa
    10681 atctactaaa aatagcaccc aacacctcat cgttagatat ttgcccagtg atttctccta
    10741 agtagtgaag gcattcgcgg atatcttggc ttacaaaatc gcccgatata cctgtagata
    10801 aacctgtttg cacacgttca atggcactta atgcttgtgt taatgcctga aaatgacgca
    10861 tattagtaat aataacatct tgatcgccaa tactaggaat agctgctgct tggtgcaaat
    10921 ggtgtaacaa ggcatcaata tgttggtgtt tagccgctga tataaataag cgttcagctg
    10981 tgatattttc aaataacaac tccaacacac ggcattcttc ttcgttaatc ttatccaatt
    11041 tattaaatac taagattaat tttttacctt cacttcgtga aactatgcgt tctgccatcc
    11101 aatcaatgtg ttcatttact tgggttgagt ctattaccca caaaatgatg gatgcttgtt
    11161 ctatttgctg ataggtacgt ttaatgccca ttttctcaat agcatcttgc gtttcgcgta
    11221 tgccagctgt atctataaag cggtacgtta tgccttgaat acttactata tcttcaatca
    11281 catcgcgcgt agtgccgtgt atatccgata caatcgcttt ttcttcgttg agtaataagt
    11341 taagcaaggt tgattttcca gcatttgttt cgcctacaat cgctaccgga atgccatttt
    11401 taatcacgtt cccaacctca aacgaacgtg ccaaactggc aagttttgtt tgtataatag
    11461 ttgctaacgc catcaagctt gacctgtcgg caaactccac atcttcttcg ctaaaatcta
    11521 attcaagctc tatcaatgca gtaaattcga gtaatttatc tctcaacact gccaattctt
    11581 tagaaaaccc accccgcatt tgattcatag ctactcggtg tgaagaggct gattgtgcgg
    11641 caattaaatc ggctacggct tctgcctgac ttaaatccat tttgccattt aagaaagctc
    11701 ttcgcgtaaa ctctcctggc tgggctaatt ggcaaccttg ttgtattagt agttgtaaga
    11761 tttgttgttg aataaacgac gagccgtggc aagctatctc taccatatct tcacccgtaa
    11821 acgaatgtgg tgctttaaat acacttacta atacctcatc tatcaattcg ttttgctgaa
    11881 aaattcgtcc aaatttgata ctatttggct gcatagtgga tagggttaca gctttgtttg
    11941 ctgcaacaaa aaacgtatcg cacatacgca atgcttcttc cccagatata cgaatgaggg
    12001 caatggctgc ttggtgttga ccagaggaaa tagcacaaat agtaggattc ataagcggtt
    12061 tatacctcaa ctgatacagt tttttagtat ctcttgaaat tatgagttaa tagtaggcgt
    12121 ttcaacctgc acttgtttta cttgagataa gcaatgtttc gtttgacaac tagaaacaga
    12181 tagcaaaaga attatggcaa ttgctattga tgtgattatt tttttcatat tctttgtata
    12241 aagagttggg attagttaaa atagcaactt acaccatgct acattggtaa ttattcattt
    12301 gtttaacgca aatgtatgaa aaaaattgta catttgcata atagaaacat tatttattca
    12361 ttataaacat aaaaaacatg ggactcttaa aggaatttaa agcttttgcc atgaaaggca
    12421 atgtaatcga cttagctgta gctgtaatta ttggtggtgc ttttggtaaa attgtatctt
    12481 cgttagtggc agatgttatt atgcctccta ttgggttatt ggtaggtggg gtaaatttta
    12541 ctgagttgag tatcgtattg aaagaagcca cagaaactac cgctgctgtt actttaaatt
    12601 atggtaattt tttgcaagca acgtttgatt ttatcattat agcttttgct atattcatgt
    12661 ttatcaaagg gttaagtaaa ttagaaaaga agaaagaaga gcctgttgtt cctgctgctc
    12721 cagttgaacc aacaccttca aaagaggaaa ttctattagc agaaatcagg gatttgttaa
    12781 aagaaaaaaa ataatcaata tcttcattac aaaaaaataa ggaacgttta aaccaacgtt
    12841 ccttattttt ttatgattgg accgtttcta cagtagcttt atctgcttca acctgaccca
    12901 atccacaatg gcaaggtgtt ttacacgagt tgaataacat agctaaaaaa gcacatacac
    12961 atactacttt tatcagattt tttttcataa tttggtatat taggttttaa ttattgtttg
    13021 taaatcaata cggtagcata cgctgccatt ccttcttttc tgcctacaaa acccatcttt
    13081 tcggaggtag ttgctttaat ggaaatatcc tcttctgaca catgcattac ctgagctaga
    13141 gtttctttca tattggcaat atgtggattg atttttggtt cttcagcaca tacagtagcg
    13201 tcaatattac tcaactcgta tccttgctct ctgagtaagt tcatcgtttt tgccagcaat
    13261 attttactat ctatgttttt aaattcgggc gatgtgtcag aaaaatggta tccaatatct
    13321 ctcaagtttg cagctccaag taaggcatca catatagcat gaattaatac atcagcatct
    13381 gaatgcccta gtaaacctag actatgttct atgctaacac cacctaacca caaggctcta
    13441 ttgggagcaa actgatgtac atcataccca aaaccaacac gaattttcat ctgtcttatg
    13501 tataaacaag tggtacttat cgagcagtta gctgtttgat gccatctaag gtaaaggcta
    13561 aactaaatcg taaagtttga tccaatgggt tattttgagc caccgaaaca acataagcta
    13621 catccaacga aaacacattg agtttgaacc ctgcaccaat agttgcatat ttacgatttc
    13681 ctttgttggc actttcgtta aaatatcctg cacgagcaaa aaactgattg ttgtaggcat
    13741 attcaagacc caatgaccac ataatttctt gcaattcttc ggagaaacca ccaggagcat
    13801 ctgagaatga ggtaaatata cctgcaatag gatccccatt attgtaatca cttaaactag
    13861 tctcatattt actctcacgc aaagcatagt cagcatcact ttcatcatct ccctgagtag
    13921 gagcaaccgg acgagaaggc accaataact tattaaggtc aagattaaca gataatttgt
    13981 tgt
//
LOCUS       DAOSRP010000046        13880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4493_length_13880_cov_5.197396, whole genome shotgun sequence.
ACCESSION   DAOSRP010000046 DAOSRP010000000
VERSION     DAOSRP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4493_length_13880_cov_5.197396"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /gene="rlmD"
                     /locus_tag="PLJ82_04220"
     CDS             complement(<1..1395)
                     /gene="rlmD"
                     /locus_tag="PLJ82_04220"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOR40745.1"
                     /translation="MRKKKNLPLLEQITITDVAAEGKAIARYNDMVIFIPFAVPGDVV
                     DIQITRKKNSYAEGKIVTTHSYSTNREAPFCEHFGICGGCKWQNLPYASQLTYKQKQV
                     YDNLTRIGKIQSFDMLPILGSEQTQFYRNKLEFTFSNKAWMNYDMLQAGTPRTAALGF
                     HIPGMFDKVLHIEKCWLQDEISNMIRNTLHEFCQQQHISYFDLKEQHGMMRNVIVRTS
                     TTGEIMVIVVFYEKDQSIINEMLSYLLKKVPSISSLLYIHNTKANDTISDQEIHVFHG
                     SDHIFEQMEGLKFKIGPKSFYQTNSRQAYELYKITRDFASLTGNELVYDLYTGTGTIA
                     NFVANRAQKVIGIEYVPEAIEDAKVNASINNITNTSFFAGDMKDILNESFIKTQGKPD
                     VIITDPPRAGMHDDVIKAILFASPKKIVYVSCNPATQARDILLLSEKYRVIKVQAVDM
                     FPHTHHIEIVAKIIL"
     gene            complement(1473..2411)
                     /locus_tag="PLJ82_04225"
     CDS             complement(1473..2411)
                     /locus_tag="PLJ82_04225"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOR40746.1"
                     /translation="MNQTAPFKVFSGTKSRYLAEKICESIGCKLGEMNIQHFADGEFA
                     VSYEESIRGDEVFLVQSTFPNSDNIMELLLMIDAAKRASARKIVAVIPYFGWARQDRK
                     DKPRVSIGAKMIADMLGVAGINRLITLDLHADQIQGFFNVPVDHLYASSIFLPYIQSL
                     KLKDLVIASPDVGGSKRANTYAKHLGVDLVLCHKTRVKANEVEGLKIIGDIQGKNVIL
                     VDDMVDTAGTITKAADIMISEGALSVRAIASHAVLSDPAIERIEKSKLTEIIFTNSIP
                     LRKESPKIRILSIADLLGDTILRVNTNESISSSYLT"
     gene            complement(2448..3728)
                     /gene="glyA"
                     /locus_tag="PLJ82_04230"
     CDS             complement(2448..3728)
                     /gene="glyA"
                     /locus_tag="PLJ82_04230"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR40747.1"
                     /translation="MKRDTAIFEIIEKEKKRQLKGIELIASENFVSEQVMEAMGSCLT
                     NKYAEGYPGKRYYGGCEVVDLSEQLAIDRLKELFGAEWANVQPHSGAQANAAVFLACM
                     NPGDKFLGLNLSHGGHLSHGSLVNSSGILYHPLEYNVKAETGRVDYDQMEEVAMREKP
                     KIIVGGASAYSRDWDYARMRKIADAVGAILMIDMAHPAGLIAAGLLNNPLKYAHIVTS
                     TTHKTLRGPRGGIILLGKDFPNPWGKTTPKGEIKMMSALLDSAVFPGIQGGPLEHVIA
                     SKAVAFGEALQPEYKVYQQQVQKNAAVLANAFINKGYKIISDGTDNHSMLIDLRAKFP
                     DLTGKIAEKALEKADITVNKNMVPFDSRSPFLTSGIRVGTPAITTRGAKDDVMLEIVN
                     LMDTVLCNPEDESIITSVRKKVNDMMTAFPLFAY"
     gene            complement(3742..6222)
                     /locus_tag="PLJ82_04235"
     CDS             complement(3742..6222)
                     /locus_tag="PLJ82_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOR40748.1"
                     /translation="MSKQSKNQENGVKAPKKPSRFFANLISFFKNEKLHFILGMVLFA
                     FAAFSVLSLLSFLTLGENDYNLLGLSFEELAEQNKQFENFGGVTGAHLANIVINKWFG
                     VSSFLLVFFIISVAYKFLNPTTSYSLFKRFIFSAFFTIWISLAFGFLLPEISQLSFIQ
                     LGGNHGNEMAKILQLSMGYSGVILLLAVSLFMFLIIGFAATLPFFQKIANFNVSSLFR
                     KKKIEETVLENSEEIEAMENSPEENDSETEEFEVFEVEEENEEKEDISFEVIEPTNED
                     AELVEQATNEEEIDSVKLEEVTNENENGVKLVIEEGVKEEIYDKIAELGEYDPTLELP
                     HYKYPTFDLLQKYDSNNKINLEEQSANKNRIIQTLDNYGIKIKEIKATVGATVTLYEI
                     IPEAGVRISKIRNLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNKTSQIVSMHSVIA
                     SKKFQEAKHELPIVFGKTITNEVFTVDLCKMPHILVAGATGQGKSVGLNAIITSLLYK
                     KHPSQLKFVLIDPKKVEFSIYSNIEKYFLAKLPDADEAIITDVTKVVQTLNSLTKEMD
                     SRYDLLKKAHVRTIIEYNNKFVKRTLNPDKGHRFLPYIVVIIDEFGDLIMTAGKDIEL
                     PIARIAQLARAVGIHMIIATQRPSTNIITGSIKANFPARVAFKVSSMIDSRTILDCPG
                     ANQLIGRGDMLISQGSDLVRIQCAFVDTPEIDNICKHIEGQRGFPSPFYLPEVDVSNG
                     ADTDMSGVDLTQRDPMFEEAARLLVIHQQGSTSMIQRKFSIGYNRAGRIVDQLEAAGI
                     LGPPDGSKPRQLLVQDEIQLERILLNLK"
     gene            complement(6335..7156)
                     /locus_tag="PLJ82_04240"
     CDS             complement(6335..7156)
                     /locus_tag="PLJ82_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40749.1"
                     /translation="MKLEEIACSRHFLLVSDADYFPINAYACALQFAQGYEKNLSFLV
                     LLTKKQLAHRDVIYETWNRQIETLQKKESIEIKVFFTESYFSELVPFTEQAETPMLFF
                     EISKNTQFNNPLSIFKQLKELRIPFVITKDSQTKPIDLSKIIVPIGFLTEEKEKAPYS
                     CNMGRFLHSEIILYQANDYGSKTTKNVESVLKLYNQFSLKVSIIKAKADSFGVEKEAT
                     MATEKMQAGMVLVSTSRDYGLDDILFGPKELHCLKRATVPIMFINPRPDLYVLCW"
     gene            7358..8719
                     /gene="hisS"
                     /locus_tag="PLJ82_04245"
     CDS             7358..8719
                     /gene="hisS"
                     /locus_tag="PLJ82_04245"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOR40750.1"
                     /translation="MKMKPTIPKGTRDFLPNEMAKRNYIFNTIKEVFRLYGFNQIETP
                     AMENLSTLMGKYGEEGDKLLFKILNSGDYLANVSADELVTKESNKLVNKFSEKGLRYD
                     LTVPFARFVVQYRDKITFPFKRYQIQPVWRADRPQKGRYREFFQCDADIVGSDSLVNE
                     VELIQLIDDVFTRLQLNVLIKLNNRKILSGIAELIGEHDKIVDITVAIDKYEKIGLEN
                     VQKELLEKGLTLHAIETITPIITLSGDNGTKLEMLKKVLATSSVGMEGIQELQTVLNY
                     VAGLNLKTAIEIDLTLARGLNYYTGAILEVKSLDVQIGSITGGGRYDNLTGVFGMQGV
                     SGVGISFGADRIYDVLNQLELYPKEKMNQTNVLVLPFGEKEQVYGLEIVKELRKNGIA
                     TELYPDVVKIKKQLAYADNNGIPYVILIGENEINTQTLTLKDMLTGEQYTGDKDTVFA
                     KIL"
     gene            8794..9522
                     /locus_tag="PLJ82_04250"
     CDS             8794..9522
                     /locus_tag="PLJ82_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOR40751.1"
                     /translation="MLKKLSLLLLLSLIVLHTTAQSDRGVVRNLQNVDLEPFHFGFIL
                     GLNYSDYTATPSNIVDTNQQIWYPASKGLSPGFTVGMLVDLRLFEYLNLRFTPTLGLG
                     QRDLNFSAFSTTTDERIGNPTIINIKSTTVDLPLLIKYSAERVGNYRPYLLAGAGPTI
                     NLARSKADPILVKPFDVQAYIGFGCDMYLEYFKLCPELKFGFGLLDSLERNHPDMQGT
                     SNQKFIDAISRLSSRMLVLTFNFE"
     gene            complement(9509..10924)
                     /locus_tag="PLJ82_04255"
     CDS             complement(9509..10924)
                     /locus_tag="PLJ82_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR40752.1"
                     /translation="MKEYIQNIVFSTIGKCADEMHQECYVIGGYVRDIFLQRPSKDID
                     VVVVGNGIELAERVCKIWGKRARLHIFKNFGTAQIKFQEMEIEFVGARKESYRLDSRK
                     PIVEDGTLVDDQNRRDFTINALAICLNQDRFGELVDPFNGVRDIENKILRTPLNPDIT
                     FSDDPLRMIRCIRFATQLKFHIEENTILALSRNKDRLTIVSKERIADEIQKIMQADKP
                     SIGWLLLDKCGLLPYILPELTAMKGVDTKSGLSHKDNFLHTLEVVDNIAKQTNNVWLR
                     WGALLHDIAKPITKKYVQGTGWTFYNHNYIGEKMVARIFNQMKLPMNEKMKYVQKLVL
                     LHMRPIALVEEGITDSAIRRLLFDAGDDIDDLMTLCEADITSKNEIKVQKFLQNLQLV
                     RKKLKDIEEKDRIRNFQPPISGQEIMEIFQLPACATVGIIKTRIKDAILDGEIPNEYE
                     EAKALTLRYGKELGLTATTQS"
     gene            11098..11820
                     /locus_tag="PLJ82_04260"
     CDS             11098..11820
                     /locus_tag="PLJ82_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40753.1"
                     /translation="MQRVDSLLLLNNIEVAKRTLDSIHVLFPQEVEERKKALLKKYEI
                     EELELERNFIFYDSIIRVKQTVIDSIEHFFVVKADSFYRGERVYEHFMQARMFPKTSL
                     KLDVTEKGTLNMSSIYSGNFPLKYTTMLISSKGVFIQLEEQSSRLYTFRDGDTYWEIV
                     NYNEKNVQDALSFINQFKQETIEITLQGQKKYSFILNNEDKNRIISTWYYATLLQEYN
                     FAKTQHNIINSKIARLREKNKK"
     gene            11849..12286
                     /gene="trxA"
                     /locus_tag="PLJ82_04265"
     CDS             11849..12286
                     /gene="trxA"
                     /locus_tag="PLJ82_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR40754.1"
                     /translation="MIRLLSPFLSIFLLLITFSLSAQTKIGKQDFLQKIWNFEVHKND
                     LVLRSDLPVIIDFYADWCGPCRMLAPELEALQREYNGRIAIYKVNVDEERELAAIFNA
                     RSLPTIFFIPKDGTPTYVMGYRSRAELKQIVDNILLSKPRIQP"
     gene            12399..>13880
                     /locus_tag="PLJ82_04270"
     CDS             12399..>13880
                     /locus_tag="PLJ82_04270"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOR40755.1"
                     /translation="MSKTKYIFVTGGVTSSLGKGIISASLGKLLKARGYNVTIQKFDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNVQTTRENNITTGRIYQNVIK
                     KERRGDYLGKTVQVIPHITNEIKQNIKQLGTKSKFDFVITEIGGTVGDIESLPFIESV
                     RQLKWELGKDCLCIHLTYVPYVAAAKELKTKPTQHSVKQLLELGVQPDVLVLRTEHPL
                     SQGIKEKVALFCNVAKSAVIECRDVPSIYEVPLRMQEEGLDEIVLSKMGLEDVQPANL
                     TQWTSFLTKLQTATEIVRIGLVGKYVELPDAYKSIIESLIHASAYNERKLKLELIQSE
                     KLTAENVTKTLSGLDGILIAPGFGDRGIEGKFIACQYARENNIPTLGICLGMQCMVID
                     IARNMLGYTDANSTEFNERTSNNVIDMMEQQKGLSVKGGTMRLGAYDCELEKHSLAYS
                     IYGKKHIQERHRHRYEFNNQYKQVFEDIGVKFSGINPDTNLAEI"
BASE COUNT         4495 a         2292 c         2348 g         4745 t
ORIGIN      
        1 caatattatt tttgcaacta tctctatatg atgtgtatgt ggaaacatat ccacggcttg
       61 tacttttata acacgatatt tttcggatag taatagaata tctcgggctt gagttgctgg
      121 attgcaactt acatatacaa tctttttggg agatgcaaac aaaattgcct taatcacatc
      181 atcatgcata cctgcacgtg gaggatctgt tataatcaca tctggtttac cttgtgtttt
      241 aatgaatgat tcattcaaga tatctttcat gtcgccagca aaaaaagatg tattggtaat
      301 attgttgata gaggcattta cttttgcatc ttctatggct tcgggtacat attcgatacc
      361 aattactttt tgagctctat ttgccacaaa gttagcaata gtaccagtgc cagtatacaa
      421 atcatacacc agttcgttgc ctgtaagtga agcaaaatca cgtgtaattt tatacaattc
      481 gtaggcttga cgactatttg tttgataaaa tgatttaggt ccgattttaa attttagccc
      541 ttccatttgt tcaaatatat ggtcagaacc gtgaaataca tgtatttctt gatcggaaat
      601 agtatcattc gcttttgtat tatgaatgta taacaatgaa ctgatagatg gcactttttt
      661 gagtaaatag cttagcattt cattgatgat tgattgatct ttttcataaa aaactacaat
      721 aaccataatc tcgcccgtag tgctagtgcg tacaataacg ttacgcatca tgccatgttg
      781 ttcttttaaa tcgaaatagc taatatgttg ttgttgacaa aattcatgta gagtatttct
      841 aatcatattg gatatttcat cttgtagcca acatttttca atatgtaata ctttgtcaaa
      901 catacctgga atatgaaaac ccaatgctgc tgttctgggc gtaccagctt gtagcatgtc
      961 ataattcatc catgctttat ttgagaacgt aaactcaagt ttattgcgat aaaattgagt
     1021 ttgttcagac cccaaaatgg gtaacatgtc aaaagattga atttttccaa tgcgtgttaa
     1081 attatcatac acctgttttt gtttgtatgt tagttgggaa gcatagggta aattttgcca
     1141 cttacatcca ccacatattc caaaatgttc gcaaaatggt gcttcacggt tcgttgaata
     1201 agaatgcgta gttactattt ttccttcagc atacgaattt tttttgcgtg taatttgtat
     1261 atctaccaca tcgccaggta cagcaaatgg aataaaaatt accatgtcat tgtagcgtgc
     1321 tatggctttt ccctcggctg ctacatctgt aatagttatc tgctctagta aaggaagatt
     1381 tttctttttt cgcatagtat tgggcgtagt gattaaatag aaataactac ccgagggtag
     1441 ctatctctat gtttgtaaat agatgtcttc tgttatgtta aataactgga actaattgat
     1501 tcattggtgt ttacacgaag aattgtgtcg cctaataagt ctgcaatact caaaattcgg
     1561 atttttggac tttcctttct tagaggaatg gaatttgtaa aaataatttc ggttagtttc
     1621 gatttttcta tacgttctat ggcaggatca gacaatacag catgcgaagc aatagcacgc
     1681 actgagagag ctccttcgga tatcattata tctgcagctt tggtaattgt gccagcagta
     1741 tctaccatat catctactaa tattacattt ttaccttgaa tgtctccaat aatctttaac
     1801 ccttctactt cgttagcttt tacacgtgtt ttatgacaaa gaaccaaatc aacacctaaa
     1861 tgcttagcat atgtattggc tcttttcgat ccaccaacat caggagatgc aatcactaaa
     1921 tcttttaatt taagcgattg gatataaggt aaaaaaatcg atgaggcata taagtggtcg
     1981 actggtacat tgaaaaatcc ttgtatttga tcggcatgta aatcaagtgt tattaagcga
     2041 ttaataccag ctacacctag catatctgca atcattttcg ccccaataga tacgcgaggt
     2101 ttatcttttc tatcttgacg agcccatcca aaataaggga taacagctac aattttacgg
     2161 gcagaagcac gtttagctgc atctatcatt agtaataatt ccatgatatt atcggaattg
     2221 ggaaatgtag attgtactaa aaaaacttca tctccgcgta tggattcctc gtatgatacg
     2281 gcaaattcac catcagcaaa gtgttgaata ttcatttcgc ctaatttaca tcctatgctc
     2341 tcgcaaattt tttcagctaa gtatctcgat ttggtgcccg aaaacacttt aaaaggagct
     2401 gtttgattta tgggttttgt tttcacttgt atatagtatt tttttgatta ataagcaaat
     2461 aaaggaaaag cagtcatcat atcgtttact tttttacgca cactagtgat gatgctttca
     2521 tcttctggat tacataatac agtatccatt aaattcacaa tttcaagcat tacatcatct
     2581 tttgcaccac gagttgtaat agcaggagta ccaacacgaa tacctgatgt aaggaatggg
     2641 ctacggctat caaaaggtac catatttttg tttacagtaa tatctgcttt ttctaatgct
     2701 ttttccgcaa tctttccagt taaatcaggg aattttgcac gtaagtcaat cagcatggag
     2761 tgattatccg ttccatcaga tataatttta tatcctttat tgataaatgc gtttgctaaa
     2821 acagctgcat ttttttgtac ttgttgttga tatactttat attctggttg taaagcttcg
     2881 ccaaaagcaa cagctttaga agcaataaca tgttccagtg gacctccttg tatgccagga
     2941 aaaacagctg agtctaataa cgctgacatc atttttattt ctcctttagg tgtggttttt
     3001 ccccatggat ttggaaaatc tttgcctaaa agaataatac cacctcttgg acctcttaat
     3061 gttttatgag tagtagaggt tacgatatgt gcatatttca aagggttgtt taacaatcct
     3121 gcagcaataa gaccagcagg gtgagccata tcaatcatca gaatagcacc aactgcatct
     3181 gcaattttgc gcatacgtgc gtaatcccaa tcacgtgagt atgcagaagc tcctcctaca
     3241 ataatttttg gtttttcacg catggctact tcttccattt ggtcgtaatc cactctacct
     3301 gtttcagctt ttacattata ctcaagagga tgatacaaaa taccagaact atttactaat
     3361 gatccgtgag aaaggtgtcc tccgtgtgat aagtttaacc ctaaaaattt atctccagga
     3421 ttcatacatg ctaaaaatac agcagcgttg gcttgtgcac cagagtgggg ttgtacgttt
     3481 gcccattcag ctccaaaaag ctctttcaat ctatctattg ctaattgctc acttaagtct
     3541 actacttcgc aaccgccgta gtatcgttta cctgggtagc cttcggcata tttatttgtt
     3601 aagcaagaac ccattgcttc cattacttgt tcacttacaa aattttcaga tgcaataagc
     3661 tcaatacctt ttagttgacg ctttttttct ttttcgatta tctcgaaaat ggctgtgtct
     3721 cttttcatgt ttgtaattat gttattttaa atttaataag atacgttcta attgaatttc
     3781 atcttgtacc aataattggc gaggtttact tccatctgga ggacctaata tccctgctgc
     3841 ttctaattga tcaacaattc taccagcacg attatatccg attgaaaatt ttcgttgaat
     3901 catcgaagtt gagccttgtt gatgaataac aagtagtctg gctgcttctt caaacatagg
     3961 gtctcgttga gttaaatcca caccactcat atctgtatca gcaccattcg atacgtctac
     4021 ttctggtaaa taaaatgggc tagggaatcc tcgctgacct tctatatgtt tgcaaatatt
     4081 atcaatttct ggagtatcaa caaaagcaca ttgaatgcga actaaatcgc taccttgcga
     4141 aattagcata tctccacgcc caattaattg attagcacct gggcaatcca aaatagtacg
     4201 agagtcaatc atcgaagata ctttaaaggc aacccttgca gggaagttcg cttttatact
     4261 tcccgtaatg atgttggtcg aagggcgttg tgtagcaata atcatatgaa ttccaactgc
     4321 tctagctaat tgggctatac gtgcaatggg taattcaatg tctttaccgg ctgtcattat
     4381 taaatcccca aattcgtcaa taataacaac gatatagggt aaaaatcggt gtcctttatc
     4441 tgggttgagt gtacgtttta caaacttatt gttgtattca ataatagtac gcacatgtgc
     4501 tttttttaat aagtcataac gactatccat ctctttggtc aacgaattca gtgtttgtac
     4561 taccttcgtt acatcagtaa tgatagcttc atctgcatca ggcaatttag caagaaagta
     4621 cttttcaata tttgagtaaa tactaaactc tactttttta gggtcaatta aaacaaattt
     4681 taattgcgaa gggtgctttt tgtaaagtaa tgatgttata atcgcattta gtcctactga
     4741 ttttccttgt cctgttgctc ctgccactaa tatatggggc attttgcaca aatcaacagt
     4801 aaatacctcg tttgtaatgg tttttccaaa tacaattggc agttcatgtt tagcttcttg
     4861 aaacttttta gaagcaataa cagaatgcat tgatactatt tgtgatgttt tgtttggtac
     4921 ttcaatacca atagtacctt tgcctggaat aggagcaata atacgaatac ctaaagcaga
     4981 caaactcaat gcaatatcat cttctaaatt gcgaattttt gaaatacgaa cacctgcttc
     5041 tggtattatt tcgtataaag ttacggtagc acctacagta gcttttattt cttttatttt
     5101 tataccataa ttatcaagcg tttgaatgat tctattttta ttagccgatt gttcttcgag
     5161 attgatcttg ttgtttgagt catatttttg tagtaaatca aacgtaggat atttataatg
     5221 tggcaactcc agggtagggt catattcgcc taattctgct attttatcgt atatttcttc
     5281 tttgacacct tcttcgatta ctagttttac accgttttca ttttcgtttg ttacctcttc
     5341 tagctttaca gagtctattt cttcttcatt agtggcttgt tctacaagtt cagcatcttc
     5401 attagtgggt tctattactt caaagcttat atcttctttt tcttcattct cttcttcaac
     5461 ctcaaatact tcaaattctt ctgtttctga atcattttct tctggagaat tttccattgc
     5521 ttcaatctct tcactatttt ctagtaccgt ctcctctatt tttttctttc taaaaagaga
     5581 actaacgtta aagttggcaa ttttttggaa aaatggtaaa gtagcagcaa aaccaataat
     5641 taaaaacata aataaggata ctgcaagcaa caatatcaca ccactatatc ccatagatag
     5701 ctgtaaaatt tttgccattt cattaccatg attaccaccc agttgaataa atgataattg
     5761 agaaatttct ggcaatagaa aaccaaaagc caatgatatc caaatggtaa aaaacgcact
     5821 aaaaataaaa cgtttgaaca aagaatatga agtggtagga ttcaaaaatt tatacgctac
     5881 tgaaatgatg aaaaagacta atagaaaaga cgaaaccccg aaccacttgt ttattactat
     5941 attagctaaa tgagccccag ttactccacc aaagttttca aactgtttat tctgttcggc
     6001 taattcttca aaacttaatc cgagtaaatt atagtcattt tctccgagag ttaaaaatga
     6061 taataaggat aatactgaaa aagccgcaaa agcaaacaat accataccca gtatgaagtg
     6121 tagtttctca tttttaaaaa aagaaataag attcgcaaaa aaacggcttg gttttttagg
     6181 agcttttact ccgttttctt gattcttaga ttgtttactc atataagggg gtgttttttt
     6241 tcttttgaag tgcaaaggta tataaaattt atctgaacaa agaaggattt tttacatagt
     6301 ttttaacaac gttttttttt attttttaca tatcttacca acacaaaaca tataaatctg
     6361 gacggggatt gataaacata atggggactg ttgcacgttt caaacaatgc agttctttgg
     6421 gtccaaatag gatatcgtcc aacccataat cgcgagatgt gcttactaat accatacccg
     6481 cttgcatttt ttctgtagcc attgtggctt ctttttctac accaaaacta tctgctttag
     6541 cttttataat gctgactttc aatgaaaatt gattgtataa ttttagtaca ctttctacat
     6601 ttttggtcgt ttttgaacca tagtcattgg cttgatacaa aataatttct gaatgtaaaa
     6661 aacgtcccat attacacgaa taaggggctt tttctttttc ttcagtcaaa aaacctattg
     6721 gaacaataat ttttgataaa tcaattggtt tggtctggct atcttttgta attacaaatg
     6781 gaatacgtag ctctttcagt tgtttaaata tgctaagtgg attgttaaac tgcgtgtttt
     6841 ttgaaatctc gaaaaacaac atcggagttt ctgcctgttc agtaaaagga actaattcag
     6901 aaaaatagga ttcggtaaaa aagactttta tttcaatcga ctcttttttt tgtagtgttt
     6961 ctatttgcct attccatgtt tcataaataa catcacggtg tgcaagttgc ttttttgtta
     7021 acaataccaa aaaagacaga tttttttcat acccttgagc aaattgtaag gcacatgcgt
     7081 aggcattaat tggaaaataa tcggcatcgg ataccagtaa aaagtgtcgt gaacaagcaa
     7141 tctcctcaag tttcatagtg tttgtcgaat atggttagtt tttggtttac aaagttagtg
     7201 ttttttcttt ttcttgtcta aataaaatgc taaaaacata tttttttgta cctttgcaac
     7261 tcgcaaaaat attgtagcga tagtttgtgt ataaacctat ctagccaaga aaacagtata
     7321 catacgacta gtattacaga gtataacatt ataatacata aaaatgaaac ctactatacc
     7381 caaaggtaca cgcgattttt tgccaaatga aatggccaaa cgaaattata tttttaatac
     7441 tataaaagag gtatttcgtt tgtacggatt taatcagata gaaactcctg ctatggaaaa
     7501 tttgtccaca ttaatgggta agtatggaga agaaggagat aaattattat ttaaaatact
     7561 gaattctggc gattatttag ccaatgtttc tgctgatgaa ttggttacta aagagagtaa
     7621 caagttagta aataaattct ctgagaaagg tttgcgttat gatttaactg ttccctttgc
     7681 tcgctttgta gtgcaatatc gcgataaaat tacgtttccg ttcaaacgat accaaataca
     7741 acccgtttgg agggctgata gaccacagaa agggcgttat cgcgaatttt ttcagtgcga
     7801 tgcggatata gtaggtagcg attcacttgt aaatgaggta gagttaatcc aattaattga
     7861 tgatgtattt acacgtttgc aactgaatgt tttaattaaa ctaaataata gaaaaattct
     7921 ttctggtatt gccgaattga ttggtgagca cgacaaaatt gtagatatta cggtagcaat
     7981 agataaatac gaaaaaattg gacttgaaaa tgttcaaaaa gaattacttg aaaaaggact
     8041 tacgctacac gcaatagaaa ccattacacc tattattacc ttatctggtg ataatggtac
     8101 aaaattagaa atgctaaaaa aagtattagc aacatcttct gtgggaatgg aagggataca
     8161 agagttacaa acggtactca attatgtagc tggactaaat cttaagactg ccattgaaat
     8221 agatttaacg ctggctcgtg gtttaaatta ctatacagga gctattcttg aagtaaaatc
     8281 gcttgatgta caaattggta gtataacggg aggtggtaga tacgacaatt taacaggagt
     8341 atttggtatg caaggtgtat ctggcgtagg tatttcgttt ggagctgacc gcatctacga
     8401 tgtactcaat caattggagt tatatcctaa agaaaaaatg aaccaaacaa atgtacttgt
     8461 attacctttt ggcgaaaaag aacaagtata cggtttggaa attgtaaaag agttgagaaa
     8521 aaacggaata gctactgaat tatatcctga cgtagtgaaa ataaaaaaac agcttgctta
     8581 tgctgataac aatggcattc cgtatgttat tttgataggc gaaaatgaaa tcaatacgca
     8641 aactctgact cttaaagata tgcttacagg cgaacaatat acaggggata aagataccgt
     8701 ttttgctaaa attctttaaa tatcatcgtt taatacaagc acaataaaat aacagtagca
     8761 tttttctgtt atactaaata gaaaaaataa actatgctca aaaaattatc cctactactg
     8821 cttttgtctt tgattgtttt acatacaaca gcacagtctg atagaggagt ggtacgcaat
     8881 ttacaaaatg tagatttaga accctttcat tttggattta ttttagggtt aaattattca
     8941 gattatactg caacaccatc taatatagtt gatacaaatc agcaaatatg gtatcctgca
     9001 tctaaggggt tatctccagg gtttacagtt ggaatgctcg ttgatttacg attgtttgaa
     9061 tacctaaatc tgcgttttac gccaacctta ggactgggac agcgtgatct aaatttttct
     9121 gcattttcta ctactacaga cgaacgaatt ggtaatccaa ccatcattaa tattaaatca
     9181 acaacagtag atttgccatt acttattaag tatagtgccg aaagagtagg gaattaccga
     9241 ccatatttgc tggctggagc tggaccaact attaatttag ctcgttcaaa agccgatcct
     9301 attttagtga aaccttttga tgtacaagct tatatcggtt ttggatgtga tatgtattta
     9361 gaatatttta agctatgtcc tgaacttaaa tttggttttg ggcttttgga ttcacttgag
     9421 cggaatcatc ctgatatgca aggaacctca aatcaaaaat ttattgatgc tataagtcgt
     9481 ttaagttcgc gtatgttagt gcttaccttt aactttgagt agttgccgtt aaccctaatt
     9541 cctttccata acgtagtgtt aaagctttgg cttcctcgta ttcattagga atttctccat
     9601 caagaatagc atctttaatg cgtgttttaa ttataccaac agtagcacat gcagggagtt
     9661 gaaatatttc cattatttcc tgaccagaaa tggggggttg aaaatttcta atcctatctt
     9721 tttcttcaat gtcttttaat tttttgcgga ctaattgaag gttttgaaga aacttttgca
     9781 cttttatttc attttttgat gtaatatcag cctcgcatag tgtcattaaa tcatcaatat
     9841 catctccagc atcaaaaaga agtcttctga tagcagagtc agtgattcct tcttcaacca
     9901 atgctatggg tcgcatatgt agtaatacaa gtttttgaac gtatttcatt ttttcgttca
     9961 taggtagctt catttggtta aaaatacgag ctaccatttt ttcgccaata tagttatgat
    10021 tataaaaagt ccaacctgta ccttgcacat atttttttgt tataggctta gcaatgtcgt
    10081 gtaacagagc accccatctt aaccatacat tattagtttg tttagcaata ttatctacta
    10141 cctctaatgt atgtaaaaag ttgtctttat gcgataatcc cgattttgta tcaacgcctt
    10201 tcatggcagt taattctggt aaaatataag gtaataatcc acatttatca agaagtaacc
    10261 aaccaataga aggtttatct gcttgcatga ttttttgaat ttcgtctgca attctttctt
    10321 tcgacacaat tgttaatcgg tctttattgc gagataaagc caatattgta ttttcttcta
    10381 tatggaactt tagttgtgtt gcgaagcgaa tacatctaat cattcttaat gggtcgtcgc
    10441 tgaatgttat atctggattt aatggtgtgc gtagaatttt attttcaata tcacgaacac
    10501 cattaaatgg atccactaat tcaccaaaac gatcttgatt taaacatata gctaacgcat
    10561 taatagtaaa atctcttctg ttctgatcat ctactagcgt cccatcttct acaatcggtt
    10621 tgcgagaatc taatcggtat gattcttttc tggctcctac aaattcaatt tccatttctt
    10681 gaaatttaat ttgagcagtt ccaaagtttt taaaaatatg taatcgagct ctttttcccc
    10741 atattttaca tactctttca gctaattcaa taccattgcc cactactact acatctatat
    10801 ctttagatgg acgttgcaaa aagatgtctc tgacataacc accaataaca taacactcct
    10861 gatgcatctc atcagcacat tttccaatag tcgaaaaaac aatattttgg atatattctt
    10921 tcatttaaat attattaacc ctacaaaagt actttcttta caccaatctg acaaaaaaat
    10981 attatttttg cctttgattt gaaggctatg aaaaaaaata tgtatgcgtg gtgtgttctc
    11041 ctgtccattt tgtttttatc atgcaataaa caggaaagaa gtacaagtgt tcattatttg
    11101 caaagagttg atagcttatt gttattgaac aatattgagg tggcaaaacg aacactcgat
    11161 tcaatccatg tactatttcc tcaagaagtt gaggaaagaa aaaaggctct attgaaaaaa
    11221 tatgaaattg aagagttaga attagaacgt aattttatct tttatgatag tataatacgt
    11281 gtaaaacaaa cagtaataga tagtatagag catttttttg tagtaaaagc tgattctttt
    11341 tacagaggtg aaagggtata tgaacatttt atgcaagcaa ggatgtttcc aaaaacgagc
    11401 ttgaaattag atgttaccga aaaaggtaca ttgaatatga gcagtatata ttcgggtaat
    11461 tttcctttaa aatatactac gatgcttatt tctagtaaag gtgtttttat tcaattggaa
    11521 gaacaatcat cgcgtttgta tacatttaga gatggagaca catattggga aattgttaat
    11581 tataacgaaa aaaatgtgca ggatgcatta tctttcatca atcaatttaa gcaagaaaca
    11641 atagaaataa cgctacaagg acaaaaaaaa tatagtttca ttttaaataa tgaagataaa
    11701 aataggatta tttctacttg gtattatgct actttattac aagaatacaa ttttgcaaaa
    11761 actcaacata atattattaa ctctaagatt gcacgtttaa gagaaaaaaa taaaaaataa
    11821 tttatttatt agtatataat aggacaatat gattagatta ctgagcccct ttttatctat
    11881 atttttattg ttgataacat tttcattatc agcacaaact aaaattggta aacaagattt
    11941 tttacagaaa atatggaatt ttgaagtgca taaaaatgat ttagtattac gttcagatct
    12001 ccctgtgatt attgattttt atgctgattg gtgtggtcct tgtaggatgc tagctcctga
    12061 attagaagct cttcaaagag aatacaatgg tcgcatagct atttataaag taaatgtaga
    12121 tgaagaaaga gaactagctg caattttcaa tgcacgatct ttacctacta tattttttat
    12181 tccgaaagac ggcacaccta cgtatgtgat gggttatcgt tcaagagctg aattaaaaca
    12241 aattgtagac aatatattgc tgtctaaacc tagaatacag ccatagattt agtgctaatg
    12301 aaaagaaata taaaaaataa aataggtttt gatttcaaaa cctattttat tttttgtaaa
    12361 tttgcaccca ttaaaaaact aatctaatag ctataattgt gtcaaaaacg aagtatattt
    12421 ttgttacagg tggtgttaca tcatctttag gtaaaggaat catatctgct tccttaggaa
    12481 aactattaaa agccagaggt tacaatgtaa ccattcaaaa atttgatcca tacataaaca
    12541 ttgatccggg tacattaaat ccatacgaac atggagaatg ttatgtaacc gttgatggtc
    12601 atgaggccga tttagatttg ggtcattatg aacgtttttt aaatgttcaa actaccagag
    12661 aaaataatat tacaactggt agaatttatc aaaatgttat aaaaaaagaa cgtagaggag
    12721 actatctagg taaaacagtt caagttatcc ctcatattac gaatgagatt aaacaaaata
    12781 ttaaacagct tggaacaaaa agtaaatttg attttgttat tacggaaatc ggaggtacag
    12841 taggagatat agaatctcta ccttttatag aaagtgttcg tcagttaaag tgggagttag
    12901 gtaaggattg tttatgtata caccttacgt atgtgccata tgtggcagca gctaaggaat
    12961 taaaaacgaa acctacacag cattctgtaa aacaactgct tgaattaggt gtacaaccag
    13021 atgttttggt gttacgaacc gaacatcctt tatcacaagg gattaaagaa aaagttgctt
    13081 tattttgtaa tgtagcaaaa tctgctgtaa tagagtgtag ggatgtaccg tcaatttatg
    13141 aagtaccatt gcgtatgcaa gaagaaggat tagatgagat tgttctttct aaaatgggat
    13201 tggaggacgt acagcctgct aatttaacac aatggacaag ttttttaaca aaattacaaa
    13261 ccgctactga aatagtgaga atagggctag ttggaaaata tgtagaattg cctgatgcct
    13321 ataaatctat cattgaatca ttaatacatg catcagcata taacgaacgg aaactaaaat
    13381 tagaacttat tcaatcggaa aaattgacag cagaaaatgt aactaaaact ttgagtggat
    13441 tagatggtat tttgattgct ccaggttttg gcgatagagg aatcgaaggt aaatttattg
    13501 cctgtcagta tgcacgtgaa aataatattc ctacattggg tatatgttta ggaatgcaat
    13561 gtatggtaat agatatagca agaaatatgc ttggctatac agacgctaat tctactgaat
    13621 ttaacgaacg tacatcaaac aatgtaattg atatgatgga gcaacaaaaa ggattgtccg
    13681 ttaaaggggg aacaatgcgt ttaggggcat atgattgtga gctagaaaag cattcgttgg
    13741 catatagtat atatggcaag aaacatattc aagaacgaca tagacatcgt tatgaattta
    13801 ataatcagta taaacaagta tttgaagata ttggagtgaa attttcaggt atcaatccag
    13861 atacaaattt ggctgaaatc
//
LOCUS       DAOSRP010000047        13801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4531_length_13801_cov_5.137858, whole genome shotgun sequence.
ACCESSION   DAOSRP010000047 DAOSRP010000000
VERSION     DAOSRP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_13801_cov_5.137858"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLJ82_04275"
     CDS             complement(<1..384)
                     /locus_tag="PLJ82_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40756.1"
                     /translation="MKKSILATFVFFAFLAVSHAQESVPFKMSAKPTITLFSNFKTGI
                     ADPNNKIGFNLERVYLGTSFLLSENLSGRVRFDIGDPKVPGSDLEMVAYVKNAEIKWE
                     AGNVTFNAGMISTIQFAVQEKFWGYR"
     gene            574..1605
                     /gene="pstS"
                     /locus_tag="PLJ82_04280"
     CDS             574..1605
                     /gene="pstS"
                     /locus_tag="PLJ82_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOR40757.1"
                     /translation="MKKLVFLLSTVTVLLSSCSTETVELAGAGATFPVPYYNVAFEAY
                     KAETGNIVSYGGIGSGGGIRSITEGIVDFAGTDAFLNDERMAEIDEVIHIPTCMGAVV
                     LSYNLEGVSKLNLTGEIIANIYLGKITNWNDSLIQAINPDVTLPTKKITPVFRSDGSG
                     TTAIFANYLSKVSPTWKDTLGEGTALNFTVGVGGKGNPGVAGTVAVTDGAIGYIGSEY
                     AFSLGLSIASIQNSKGEFIQPNTASISAAAQGDIPADTRVMITNSEATGSYPISGFTW
                     IVVYKEQAYANRSIEKAEATVELLHFLLSEKAQSLTETVHYAPLSEAVIALTKANIKK
                     MTYQGNPIE"
     gene            1626..2480
                     /gene="pstC"
                     /locus_tag="PLJ82_04285"
     CDS             1626..2480
                     /gene="pstC"
                     /locus_tag="PLJ82_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOR40758.1"
                     /translation="MSDKAFKTLLWFAASIILIITGGMLYSLTSGAIPAFKQFGFFEF
                     LSSSEWNPTEGSEKYGALAFIIGTIITSILGILFCLPFSLPVALFIGEYFKGKKIASL
                     LGTIVDLLAGIPSIVYGLWGFYALRPIIMSLGLSPQGFGILTASLVLGIMIIPYAASL
                     SAEFIRMVPNDLKEGAYSMGATRAEVIQHIILPISGSGIFSSYILALGRALGETMAVT
                     MLIGNANGIPNSIFDTGNTMASIIANQFGEAYGLKLSSLIAVGLLLFLITAIINFIGK
                     LIIKRNNL"
     gene            2477..3343
                     /gene="pstA"
                     /locus_tag="PLJ82_04290"
     CDS             2477..3343
                     /gene="pstA"
                     /locus_tag="PLJ82_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOR40759.1"
                     /translation="MNTFTYRKIKNASLYVLVCLFAGLTAVPLFAILGQVLIKGWREL
                     IVSDFFVKSTPTTLDALIALRSGEIIPGGIANGIVGTILMVLMATFIAIPIGIFAGIY
                     LAEYKTSKYASIIRFLTDLLQGVPSIVVGVVGFIWVVIPLKSYSALAGSAALTVMMLP
                     LIIRSTEETMKMIPNTMKEAGLALGTSYPIIILRVMLPSAFGGIFTGVLLAVSRVIGE
                     TAPLMLTALGSTMIQWDVTRPNSAVSLLIWEFYNDPNLQSLVWSASLFLLIFVLTLNI
                     IAKQIAKKWRIQ"
     gene            3328..3981
                     /locus_tag="PLJ82_04295"
                     /pseudo
     CDS             3328..3981
                     /locus_tag="PLJ82_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
     gene            3961..4368
                     /locus_tag="PLJ82_04300"
     CDS             3961..4368
                     /locus_tag="PLJ82_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR40760.1"
                     /translation="MSQAARIVDIQNNLENNLDIVGNKSLIFAIFRNLLENAINYAGE
                     EITIGIKCYNEDADYYYFLFYDTGCGVKPECLEKIFDRFVRIDQGRSRRNGGTGLGLS
                     IVKHAVMFHNGAIEARNRKTGGLEFYFTLKKRK"
     gene            4584..4946
                     /locus_tag="PLJ82_04305"
     CDS             4584..4946
                     /locus_tag="PLJ82_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOR40761.1"
                     /translation="MKKYLITVNQKQYEVEVVEVKQQVQTQKTTSGIKPSVTVSKPKE
                     TVAGANGHKVAAPMPSNIIKLLVNEGDVVNEGDNLLVFEAMKMENNLHSPVSGKIASI
                     KVKVGNSVAAGDTLIIIE"
     gene            4959..6527
                     /locus_tag="PLJ82_04310"
     CDS             4959..6527
                     /locus_tag="PLJ82_04310"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOR40762.1"
                     /translation="MGKVKITETALRDAHQSLIATRMRTEDMLPILEKMDNIGYHSLE
                     AWGGATFDSCIRFLNEDPWDRLRKIKQVVKKTPLQMLLRGQNLLGYKHYADDVVEYFV
                     QKAIDNGMDIIRIFDALNDPRNIETAIKACIKEGGHAQACVCYTTSPVHNLELFAKDA
                     KTLENMGAHSICIKDMAGLLKPYDAYELVKAIKETVNIPVQLHTHYTNGVASMTLLKA
                     IEAGVDVVDTAISPMAMGTSQPPTEPLVASLQGHERDTELDLVKLSEIAEYFRPLKEK
                     WLESNMLDTKVMGVDVNALIYQVPGGMLSNLVSQLKQANAMDKYEEVLREVPRVREDF
                     GYPPLVTPSSQIVGTQALFNVLMGERYKMVPQESKGIIKGEYGKTPAPISDEFRKKIL
                     GDEEAITCRPADLIAPELEKTREAIKDLMIQEEDVLTYALLPQVAEKFFQMRKNGTLE
                     AVKEEQKKQATPVVASSKPAQETAESNDDEDEVAAVMSAAIATIESENGGGQFAITSV
                     RQVSPWVISARLNR"
     gene            complement(6608..8764)
                     /locus_tag="PLJ82_04315"
     CDS             complement(6608..8764)
                     /locus_tag="PLJ82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR40763.1"
                     /translation="MKVYQTNEIKNISLVGSSGSGKTTLTEAMLFESGVIKRRGSIEA
                     KNTVSDYFPVEHEYGYSVFPTVFHSEWMGRKLNMIDCPGADDFVGGLITSLSVTDTAL
                     MLINAQHGVEVGTQNQYRQIEKLKKPCIFVVNQLDHEKADFDKTVDDLRQNFGNKVVL
                     IQYPIQTGPAFNAVVDVLLMKLYTWKADGGVPTISDIPANELQRASELHNILVEAAAE
                     HEENLMEKYFADGNLTEDEMRMGIRFGLVHRDIFPVFCISAAKDMGVRRLMEFLGNVV
                     PYVRDSAAPVATKGEEVAPLQENNASIYVFKTSVEPHIGEVSFFKVMSGKVKEGDDLY
                     NATRQSKERLTQIYAVCGSIRTKVDELVAGDIGATVKLKNTRTSNTLNGKNADLQFDF
                     IKYPEWKFRKAIAAVNQADEEKLSEVLTRMHEEDPTWIIEYSKELKQTIVHGQGEFHL
                     KTLKWRIENNDKILINYSNPRIPYRETITKTARADYRHKKQSGGAGQFGEVHLIIEPC
                     VEGVPEKEVYKINGQEIKISVRDKQEYPLEWGGKLVFINSIVGGVIDARFLPAIQKGI
                     MDCMEQGPLTGSYARDIRVIVYDGKMHPVDSNEISFRLAGKYAFSEAFKNAGPKVLEP
                     IYDVEVLVPSASMGDVMSDLQGRRALIMGMESDKGMEKIFAKVPLKEMNNYSISLSSN
                     TRGRASFSMKFASYELLPSDVQDALLKEYEKSKVAE"
     gene            complement(9130..10230)
                     /gene="ychF"
                     /locus_tag="PLJ82_04320"
     CDS             complement(9130..10230)
                     /gene="ychF"
                     /locus_tag="PLJ82_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOR40764.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVI
                     TVPDERLNKLAELVKPQRIVPTTVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFENENITHVDGSINPVRDKEIIDTELQLKDLETVESRIAKVQKQAQTGGDKTAK
                     AVYDIYMQYKEALLQGKSARTVQLDKEQAKLVKDLCLLTNKPVMYVCNVDEASAVKGN
                     AYVDAVREAVKDENAEILIVAAATEADIAELETYEERQMFLEEVGLSESGVARLIKSA
                     YKLLNLETYFTAGVQEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRAEVIKYIDFTTLG
                     SEAACKEAGKMSVEGKEYVVQDGDIMHFRFNV"
     gene            10399..11292
                     /locus_tag="PLJ82_04325"
     CDS             10399..11292
                     /locus_tag="PLJ82_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40765.1"
                     /translation="MNKSTIIRFSALFIAIFVVVTFLFVYERKKNNEKEAELNQIIAE
                     LEISKQESLDEFENLALEYENYHFKPGNDSLLFLIENEKQKVKQLLEELRTVKATNAR
                     RIRELRNELGSVRSVLQVYVNRVDSLSNVNKKLVRENKKVQQQYEEVVQMAKELEERA
                     SQLDKKITLAAILEASNIRLNTLNDKGRETKRRSRIAAFEVCFSLSKNQTTSTGLKSA
                     YLRISNPQGNVMKNNQSNVFAFEDKEIDFSCKKEIEYTGNNLSTCVYYNIADEELVAG
                     LYEVTIFIDGNLIGSESFYIK"
     gene            11299..12429
                     /gene="hemW"
                     /locus_tag="PLJ82_04330"
     CDS             11299..12429
                     /gene="hemW"
                     /locus_tag="PLJ82_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOR40766.1"
                     /translation="MAGIYIHIPFCKHRCGYCDFYSHTNLQLKQRYVEALCKEIELRK
                     HYLTPNELIETLYFGGGTPSLLNTSDFEQIYNALSTHFSLHNLVEITVEVNPDDVNDL
                     LLDTLKSIGCNRLSMGIQSFFDHELTVLGRRHTAQQAIEAVNLAQQHGFSNISIDLMY
                     ALPNQTIQTWRETLKLAISMNVQHISAYHLTYEPGTLFYKQKTKKIITEKDEEESIQL
                     YEILIQSLTEAGYNQYEISNFSLPDYESKHNSSYWKNTPYIGLGASAHSYNGASRQWN
                     IASVITYCNQIEENKSFFEIEFLTEKEKYNDFIITSLRTSKGVDLQKLTTLFGEKSRS
                     YFIQQCQPYVHTNMLCEKDNFVRLTQKGIFVSDSILETLIDV"
     gene            12440..12811
                     /locus_tag="PLJ82_04335"
     CDS             12440..12811
                     /locus_tag="PLJ82_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOR40767.1"
                     /translation="MLQFVRSYWKTIVTAIFIAYLSLFKPNSTIQLPPIPFVDKWIHI
                     VLYTTLTAVALLDTKKRKNNTSSFLLVLLISILYGGLMEILQQFFPPRTSSWYDFLAN
                     ILGCLLAFGSITWFVKKKRNG"
     gene            12804..>13801
                     /gene="dprA"
                     /locus_tag="PLJ82_04340"
     CDS             12804..>13801
                     /gene="dprA"
                     /locus_tag="PLJ82_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOR40768.1"
                     /translation="MDNKLLYQIAITQIKGLGSINSKNLLAYVGDIEEIFHQKSHILQ
                     KIPGIGILLSKSILEQKDAALEKATKEIQFITKHGITPLFYTDKNYPERLKECPDAPI
                     LLYYKGNTNLNTQKTLAIVGTRNATEYGKTMCTQLIKELANNHPELCIISGLAYGIDI
                     CAHKEALHMHLPTVAVLGHGLDRIYPAQHATIAKKILTSGGLLTEFPSETNADKPNFV
                     RRNRIIAGTTDATLIVESAARGGAIITTEIANSYNRDVFAVPGKCTDEWSVGCNNLIK
                     QHKAALVNSASDIEYIMGWTKEQTSQKIEQQQSLFIDLSDTEQEIFTIIKQNKVVH"
BASE COUNT         4550 a         2661 c         2415 g         4175 t
ORIGIN      
        1 gcggtaaccc caaaactttt cttgaacagc aaattgaata gtgctaatca tacccgcatt
       61 gaatgttaca ttaccagctt cccatttaat ttctgcattt tttacatacg ccaccatttc
      121 taagtcagaa ccaggaactt tgggatctcc tatatcaaaa cgtacgcgac cagataagtt
      181 ttcacttaat aaaaaggatg tgcccaaata aactctttct aaattaaaac ctattttatt
      241 attcggatct gctattcccg ttttaaaatt actaaataga gtaatggtag gttttgcact
      301 cattttaaaa gggacagact cttgtgcatg agacactgct aaaaaagcaa aaaatacgaa
      361 tgtagctaaa attgattttt tcatatcctg ataattattt atttcgtagg caaaagtata
      421 taaagttttt ttatcttgtt acacgcacca ttctatcctt tcacatctta acatcttcgt
      481 aatatttgcg taacatttcc gcaacaagtt agttgtttct ttgcattgta aactttttat
      541 tcatatttaa ttaaacattt agcaacagaa aacatgaaaa aactagtatt tttattgagc
      601 actgttacag ttttgttaag cagctgtagc accgaaacag tagaacttgc aggagctgga
      661 gcaacctttc ctgttccata ttataatgta gcctttgaag cctacaaagc agaaacagga
      721 aatattgtat catacggagg aattggtagt ggcggtggaa ttcgcagtat taccgaagga
      781 atcgttgact tcgccggaac agatgcattt ttgaacgacg aaagaatggc tgaaattgac
      841 gaagtaatac acatacctac atgtatgggt gctgtggtgt tatcatataa tttagaaggc
      901 gtctcaaaat taaatcttac aggagaaatt attgccaata tctacttagg aaaaattaca
      961 aactggaatg attcattgat acaagccata aatcctgatg taacattacc tactaaaaaa
     1021 attactcccg tattccgttc agatggtagt ggaacaaccg ctatttttgc aaattattta
     1081 agcaaagtta gccctacatg gaaagataca ctgggtgaag gtactgctct taatttcact
     1141 gtaggagtag gtggaaaagg gaatccagga gttgcgggaa cagtagctgt aacagatgga
     1201 gcaattggtt atattggttc ggaatatgct ttttcattgg gcttatctat agcatctatt
     1261 caaaactcaa aaggagaatt tatacaacca aatacagcat ctatttcagc tgctgcacaa
     1321 ggcgatatac ctgctgacac gcgcgttatg ataacaaact cagaagccac aggttcgtat
     1381 cctattagcg gattcacttg gatagttgta tataaagaac aagcttatgc caatcgtagt
     1441 atagagaaag cagaagcaac tgttgagttg ttacatttct tgttgagcga aaaagctcaa
     1501 tcacttacag aaactgttca ttatgccccc ctttcagagg ctgtaattgc tttaacaaaa
     1561 gcaaatatta aaaagatgac ctaccaaggt aatccaatag aataataaac taacaataca
     1621 acgacatgtc tgataaagcc tttaaaacgc ttttatggtt tgccgcctca atcattctga
     1681 ttataactgg aggtatgctc tactcactta cgagtggagc tatacccgcc tttaaacaat
     1741 ttggtttttt tgaattttta tcatcttcag aatggaatcc aacagaggga agtgaaaaat
     1801 atggtgccct tgcatttatc atcggcacca ttattacttc aatcttggga attttatttt
     1861 gtttaccctt ttcgttacct gtagcacttt ttattggtga atattttaaa gggaaaaaaa
     1921 ttgcgtcact attaggcact atcgttgatt tattagcagg tatcccttcc atcgtatatg
     1981 gactttgggg cttttatgcc ttacgtccta ttattatgtc gttaggtctt tcccctcaag
     2041 gatttggaat tttaacagct tcgctcgtat tggggattat gattataccc tatgcagcat
     2101 cgttaagtgc tgaatttatt agaatggttc caaatgattt gaaagaagga gcttatagta
     2161 tgggtgctac gcgtgcagaa gtaatccaac atattatttt gccaatttct ggttcaggta
     2221 tcttctcttc ctatattttg gcacttggaa gagccttggg agaaaccatg gctgttacaa
     2281 tgcttattgg caatgccaac ggtataccta actctatttt tgacactgga aataccatgg
     2341 caagtattat tgctaatcaa tttggagaag cttacggact aaaattatct tcactaattg
     2401 cagttggttt gttacttttt cttattacag ccataatcaa ttttattggt aaactgatta
     2461 ttaaacgcaa caacttatga acacgtttac gtatagaaaa atcaaaaatg catctttata
     2521 tgtgctcgtt tgcttgtttg caggacttac ggctgtgccc ttatttgcca ttcttggtca
     2581 agtactgata aaaggctggc gagaactaat agtaagtgat ttttttgtaa aatcaactcc
     2641 taccacatta gatgcattaa tagcattgcg ttcgggcgaa ataatacctg gagggattgc
     2701 aaatggtatt gtgggaacca ttttgatggt actaatggct acttttattg ctattccaat
     2761 tggtatattt gctggcattt atcttgcaga atacaaaaca agcaaatatg cctctattat
     2821 tcgattctta acagacctat tacaaggcgt accctctatt gttgtgggcg tagtagggtt
     2881 tatttgggta gtaattccac tcaaaagtta ttctgctctt gcaggcagtg ccgctcttac
     2941 ggttatgatg ctaccgttaa ttatacgttc taccgaagaa accatgaaaa tgatacctaa
     3001 cacaatgaaa gaagctggat tagcactagg gacatcctac ccaattatca ttttgcgagt
     3061 gatgttacct tctgcatttg ggggtatatt tactggcgtt ttgcttgctg tatcacgtgt
     3121 tattggcgaa acagcccctt taatgcttac tgcacttgga agcacgatga tacaatggga
     3181 cgtaacacga ccaaattcag cggtatcttt actaatatgg gaattttata acgatcctaa
     3241 cttacaaagc ttagtatgga gtgcttcgct atttttatta atttttgtat taacactcaa
     3301 tattatcgcc aaacaaatag ctaaaaaatg gagaatacaa taaaagaaaa tatattaaca
     3361 ctcaaagacg tttgtatatc ctatactcct ggacaaaatg cagtgaatat ggttacagct
     3421 gatatacaaa aagggcttat cacgtccatt ataggacctt cgggatgtgg taaaagtacg
     3481 cttctgaggg caatcaatcg catgcacgaa ctttatccag gcattaccat tactggagat
     3541 atcaaactaa acaacaaaga tatcttaaaa atgaacgcca tagaagtgcg acgtatggca
     3601 ggtatggtgt ttcaacgtcc taatcctttt cctacattaa gcatctatga caatgttata
     3661 gcaggttata ccctcaatgg tattaaaaaa acaaaagacg aaaaagatga aattgttgaa
     3721 aatagcctaa agaatgttgc attatgggat gaagtaaaag acgtattaca aaaaaaagga
     3781 accttccttt ctggaggaca acaacaacga ttatgtattg ctcgtgcttt ggctctaaaa
     3841 ccagatatat tattaatgga tgaaccaacc tcagcactag accccatagc taccaataga
     3901 attgaagagt tactggtaga actaaaaaaa gaggttacca tcgtcattgt tacacacaat
     3961 atgtcgcaag cagcacgtat tgttgacatt caaaacaact tggaaaacaa ccttgatatc
     4021 gttggaaaca aaagccttat ttttgccata tttcgtaatt tactcgaaaa tgccatcaac
     4081 tatgctggcg aagaaatcac catcggcatc aagtgttaca acgaagacgc tgactactat
     4141 tatttcttgt tttatgatac cggttgtggc gtaaaacctg aatgtctaga aaaaatattc
     4201 gatcgctttg tacgtatcga ccaaggacgg agtagacgca atggcggtac aggactagga
     4261 ctatccattg taaaacacgc tgttatgttt cataatggag ctatcgaagc ccgaaataga
     4321 aaaacaggag gactagaatt ttattttact cttaaaaaaa gaaaataaat attgtatcat
     4381 tcttataaaa tagaaacgct cttttctact gaaaactatg taattcggta aaaaagagcg
     4441 ttttggtcgt taatattttc taaaagtaaa acagattact aaaaaagctg tacttttgca
     4501 tagtaaatta tctcttcacg agataaccta ctgagaataa aaacatacat accaattaaa
     4561 acattattaa agacaattta ttcatgaaaa agtatctcat taccgttaac caaaaacaat
     4621 acgaagtaga agttgtggaa gtaaaacaac aagtacaaac gcaaaaaaca acttctggca
     4681 tcaaacctag tgtaactgta tcaaaaccta aagaaacggt ggcgggagca aatggtcata
     4741 aagttgcggc acccatgcct agcaatataa ttaaattgtt ggtaaacgaa ggagacgtag
     4801 taaacgaagg agataatcta ttggtatttg aagccatgaa aatggaaaac aacctacact
     4861 ctccagtaag cggaaaaatt gcctctatca aagtaaaagt tggcaattct gtagctgcgg
     4921 gagacacatt aatcatcata gaataagaca ctacaacaat gggaaaagtt aaaattacag
     4981 aaacagcatt acgtgatgca catcaatcgc tgatagctac acgtatgcgt acagaagata
     5041 tgctccccat tcttgaaaaa atggataata tcggctacca ctcgctcgaa gcttggggtg
     5101 gtgctacatt cgattcttgt atccgtttcc taaacgaaga cccttgggat aggctacgga
     5161 aaattaaaca agttgtaaaa aagactcctt tacaaatgtt acttagaggt caaaacctcc
     5221 taggttacaa acattatgct gatgatgtag tggaatattt tgtgcaaaaa gcgattgata
     5281 atggtatgga tatcatccgc atatttgatg cacttaacga ccctcgcaat attgaaaccg
     5341 ctattaaagc atgtatcaaa gaaggtggtc atgcacaagc gtgtgtttgt tataccacta
     5401 gccctgtgca caatttagaa ctatttgcta aagacgctaa aacgctcgaa aacatgggag
     5461 cccattccat ctgtatcaaa gatatggctg gcttgttgaa accatacgat gcgtacgaac
     5521 tcgtaaaagc aataaaagaa accgttaaca ttcctgttca attacatacg cattacacca
     5581 atggtgtagc ctctatgacc ttgctaaaag cgatagaagc aggcgttgat gtggtggaca
     5641 cagccatttc gcctatggcg atgggtacgt ctcaacctcc taccgaaccg cttgttgcct
     5701 cattgcaagg acatgaaagg gatactgagt tagatttggt aaaactgagc gaaattgccg
     5761 aatatttccg tccgttgaaa gaaaaatggc tcgaatcaaa catgttggat accaaagtaa
     5821 tgggagtaga tgtaaacgct cttatttatc aagtacctgg cggtatgttg tcgaacttag
     5881 tttcgcaact taaacaagcc aacgctatgg ataaatacga agaagtgctt cgtgaagtac
     5941 ctcgtgtacg cgaagatttc ggctatcctc cgctagttac tccatcgagc caaattgtgg
     6001 gtacgcaagc tcttttcaac gtattgatgg gcgaacgata caaaatggtg ccacaagagt
     6061 caaaaggtat tattaaaggc gaatatggta aaactccagc tccaatatca gacgaattta
     6121 gaaaaaaaat tctaggagat gaagaagcca ttacctgccg accagctgat ttgatagctc
     6181 cagaattgga aaaaacacgc gaagctatta aagatttgat gatacaagag gaagatgtac
     6241 taacatatgc cttactgcca caagtagcag aaaaattctt ccaaatgcgt aaaaatggta
     6301 cgcttgaagc cgtaaaagaa gaacaaaaaa aacaagcaac tcctgttgtc gcatcttcaa
     6361 aacctgcaca agagacagca gaaagcaacg atgacgaaga tgaagtagct gcagtaatgt
     6421 ctgccgccat agctactatc gaaagcgaaa atggtggagg tcagtttgca ataacttctg
     6481 ttcgccaagt aagcccttgg gttatttccg ctcgactaaa tagatagcat tttacatgac
     6541 agatagtaaa taaggcgttt cagaaattct gaaacgcctt atttttgttc aaaaactaaa
     6601 tcattattta ttcagccacc ttgctctttt cgtactcttt aagcaaggca tcttgcacat
     6661 cagaaggcag taattcgtaa gaagcaaatt tcatgctaaa agaagctctt ccccgagtat
     6721 tagaactcaa cgaaatggaa taattgttca tttcttttaa tggaactttc gcaaatattt
     6781 tttccatacc tttgtctgat tccattccca ttatcaacgc acgacgtcct tgcaaatcgc
     6841 tcattacatc gcccatactt gcactaggca ctagcacttc tacatcgtat ataggctcta
     6901 atacttttgg tcctgcattt ttaaaagcct cgctaaaagc atatttaccc gccaaacgga
     6961 aagatatttc gttagaatct actgggtgca tttttccatc atacacaatt acacggatat
     7021 cacgtgcata cgaacctgtt agcggacctt gctccataca atccattatt cctttttgaa
     7081 tagcaggcaa aaatcgagca tcaatcacac cacctacaat actgttaata aacacaagct
     7141 tcccacccca ctctagtgga tattcttgtt tatcacgtac agaaattttt atttcctgtc
     7201 cattaatctt atacacttct ttttcaggaa caccttctac acatggttca ataatcaaat
     7261 gtacctcgcc aaactgaccc gcaccacccg actgtttttt atgtcggtaa tccgctcgag
     7321 ctgttttcgt aatggtttct ctgtatggaa tacgtggatt tgaataatta atcaagattt
     7381 tatcgttatt ttcgatacgc catttaagtg ttttgagatg aaattctcct tgtccatgaa
     7441 caatcgtttg ttttaattct ttagagtatt caattatcca agttgggtct tcctcgtgca
     7501 tgcgagttaa cacctcactt agtttttcct catcagcctg attaaccgct gcaattgctt
     7561 tacggaattt ccattcggga tatttaataa aatcaaactg taagtcagca ttttttccgt
     7621 ttaaggtatt ggatgtacgc gtatttttga gcttaactgt ggcaccaata tcccctgcta
     7681 ctaattcatc cacttttgtt ctaatacttc cacacacagc gtagatttga gttaatcgct
     7741 ctttgctttg gcgagtggca ttatacaaat catcgccttc ttttactttt ccagacatca
     7801 ccttaaagaa agagacctcg ccaatatgcg gctctacaga ggttttaaac acataaatag
     7861 aagcgttatt ttcttgtaat ggagcaacct cttctccctt agttgctaca ggagctgccg
     7921 aatccctaac atagggaact acattcccta aaaattccat caaacgacgc acgcccatat
     7981 ccttggcagc actaatgcaa aaaacaggaa aaatatcacg atgcaccaag ccaaaacgaa
     8041 tacccatacg catttcatct tcagtcaaat taccatcggc aaaatatttt tccatcaaat
     8101 tttcctcatg ttctgcagct gcctccacta aaatattgtg caattcggaa gctcgttgaa
     8161 gttcattggc tggaatatcg ctaatcgttg gtacgccacc atcagctttc cacgtataca
     8221 acttcattag caatacatct acaacggcat taaaagcagg tcccgtttga atcggatatt
     8281 gaataagtac cactttattg ccaaaatttt gacgcaaatc atccactgtt ttatcaaaat
     8341 ctgctttttc atggtcaagt tgattcacga caaatatgca tggtttttta agtttctcta
     8401 tttgcctgta ttgattttgc gtacctactt ctacaccatg ttgtgcatta ataagcatta
     8461 gcgcagtgtc ggtaacacta agcgaagtaa tcaaaccgcc cacaaagtcg tcagcacctg
     8521 gacaatctat catattcagc tttctgccca tccattcaga atgaaaaaca gttggaaaaa
     8581 cagagtaacc atactcatgt tcgacaggaa aataatcaga aaccgtattt tttgcttcaa
     8641 tactgcctct acgttttata actccactct caaagagcat tgcttcagtg agcgtggttt
     8701 ttcctgagcc agaagatccc acaagtgaga tattcttaat ttcatttgtt tgatacactt
     8761 tcatacaatt attggtatta aggttactaa attgttgtaa acgaatccct cgaaagcaaa
     8821 aaaaatgcct tgcaatgtag gaaatagtta gtaaaaaaac aatagtgaaa atatgttgtg
     8881 taacaatatt tgtaacaatt ttgtaatatg aagccaaaaa tctgttaatt taaagactta
     8941 gtaaaccaat attttgtagt taattgtatt ttgcccgtat attcgcatat gattttttca
     9001 tagtatttga ttttaaggtt agcaagtagg tagtcaggcg agactacctt ttgtttttta
     9061 taggggtaat aaaaaaaggg ggaaacaatg tttccccctt tttgattctt ttattaaaca
     9121 gatattttgt tacacattaa acctaaaatg cataatatct ccatcttgta caacatattc
     9181 ttttccctca acgctcattt ttccagcctc tttgcaagca gcttctgatc ccaaggttgt
     9241 aaaatcaata tacttaatta cttcggctct aataaaacct ttttcaaaat cggtatgaat
     9301 aacacctgca cattgtggtg ctttccagcc cttttcgtag gtccaagccc tcacttcttg
     9361 cacacctgct gtaaaataag tttctaaatt tagcaatttg taagccgatt taattaatcg
     9421 tgctacaccc gactccgata atcccacttc ttctaaaaac atctgacgct cctcgtaggt
     9481 ttctaattcg gcaatatctg cttcggtagc agccgcaaca atcaatattt cagcattttc
     9541 atcttttacc gcctcacgca cggcatctac gtacgcattg ccctttacgg cagacgcttc
     9601 gtcaacgttg cacacgtaca tgactggttt attggtaagc aaacataagt cttttaccaa
     9661 tttagcttgt tccttatcta actgtacagt tcgggcagat ttgccttgca acaacgcttc
     9721 tttgtattgc atataaatat cgtatacggc tttggctgtt ttatcgccac cagtttgtgc
     9781 ttgtttttgc acttttgcaa tacgcgattc tactgtttct aaatctttta gctgcaattc
     9841 tgtatcaata atttctttgt ctctaacggg gttaattgac ccatccacgt gggttatatt
     9901 ttcgttttcg aaacaacgca atacgtgcaa aatagcgtct gtttcgcgaa tattagctaa
     9961 aaatttattg cccagccctt cgcctttgct tgctcctttt accaaaccag caatatctac
    10021 aatctccact gtggttggca caatacgttg cggtttaacc aattcagcta atttatttag
    10081 cctttcgtcg ggcactgtta taacacccac attcggttct attgtacaaa aggggaaatt
    10141 agccgcttgg gctttagcgt ttgataaaca attaaaaagg gtcgattttc ccacgttggg
    10201 tagtccaact attccacatt gtaaagccat gatacttgag ttaaaatttt gagaagacaa
    10261 aggtaataaa aaagacataa cttttttcat ttctttgcta tcgttcatat ttattatgag
    10321 caatagtagc acaatatgtt gttgatacga tgttttttgc tacttttgca tcaatttcag
    10381 aaatcttcat aatttgtcat gaacaaaagc accatcatac gtttttctgc actttttatt
    10441 gcaatatttg ttgtagtaac gtttttattt gtatatgaac gtaaaaaaaa caacgaaaaa
    10501 gaggctgaac ttaaccaaat aattgcagaa ctagaaatta gtaaacaaga atcgcttgac
    10561 gaatttgaaa atttggcact agaatacgaa aactatcatt tcaaaccagg caatgactcg
    10621 ctcttgtttt tgatagaaaa cgaaaaacaa aaagttaaac aattgctcga agaattacgt
    10681 actgtaaaag cgactaatgc tcgtcgcata cgagaattgc ggaatgaatt gggtagcgta
    10741 aggtctgtat tgcaggtgta cgttaaccga gtagattcgc taagcaatgt aaacaaaaaa
    10801 ctagtacgcg aaaacaaaaa agtacaacaa caatacgaag aggtagtgca aatggctaaa
    10861 gaattggaag aaagagcctc gcaattggat aaaaaaataa cccttgcagc cattttagaa
    10921 gcaagcaata ttcgcctaaa caccttaaat gataaaggga gagaaacgaa aagaagaagc
    10981 agaatagccg cttttgaagt atgtttctcg ctatcaaaaa accaaacgac tagcactggt
    11041 ctaaaatcgg catatttacg catatccaac ccacaaggaa acgtaatgaa aaataaccaa
    11101 agtaatgtat ttgctttcga ggataaagaa attgattttt cgtgcaaaaa ggaaattgaa
    11161 tatacaggaa acaacctttc tacttgcgtt tactacaaca ttgccgacga agaactagta
    11221 gctggcttat acgaagtaac tatttttatt gatggtaact taattggttc agaaagtttt
    11281 tacattaaat aaaacactgt ggcaggtatt tatatacata taccattttg caaacatcgg
    11341 tgtggatatt gcgattttta ttcgcatacc aacttacaac tcaaacaacg gtatgtagaa
    11401 gccttgtgca aagaaattga actccgaaag cactatctaa ctcccaacga acttattgaa
    11461 actttgtatt ttgggggtgg aacaccaagc ttgctcaata ctagcgattt tgaacaaatt
    11521 tacaatgctc ttagcactca cttttcgcta cacaacttag ttgaaataac agtagaagtg
    11581 aatccagatg acgtgaacga tttgttgtta gatacactca aatccatagg ttgtaataga
    11641 ttgagtatgg gtattcaatc gtttttcgac cacgaactta ctgttttagg acgcaggcat
    11701 accgcccaac aagccatcga ggcagttaat ttggctcaac aacatggttt ttcgaatatc
    11761 agtatcgacc ttatgtatgc cctacccaac caaacaatac aaacgtggag ggaaacatta
    11821 aaactagcca tttcgatgaa tgtgcaacat atttcggcgt atcatttaac ttacgaacct
    11881 ggcacactct tttacaagca aaaaacgaaa aagataatca ccgaaaaaga cgaagaagaa
    11941 agtattcaat tgtatgaaat ccttatacag tcgttaaccg aagctggtta taatcaatac
    12001 gaaatttcga atttttcact ccctgactac gaatcaaaac acaattcatc gtactggaaa
    12061 aatacaccct atattggatt gggtgcttcg gctcattcct acaacggagc atctcggcaa
    12121 tggaacatcg catctgtaat tacctattgc aaccagattg aagaaaataa atcatttttc
    12181 gaaattgaat tcttaacaga aaaagaaaaa tacaacgatt ttatcatcac ttcccttcgt
    12241 actagcaaag gggttgattt gcaaaaactt actacgctat ttggagaaaa atcaagaagt
    12301 tattttatcc aacaatgcca accatacgta cataccaaca tgctttgtga aaaagataat
    12361 tttgtgcgtc ttactcaaaa aggtattttt gtatccgatt ctattttaga aacattaatt
    12421 gatgtgtaac aactctgcaa tgctacaatt tgtacgttca tattggaaaa ccattgtaac
    12481 agcaatcttc attgcgtatt tgtcgctatt caaaccaaat agcaccattc aactacctcc
    12541 tattcctttt gtagataaat ggatacacat tgttctgtat actacgctaa cagcagtagc
    12601 cctacttgac acaaaaaaaa gaaaaaataa tacgtcatca tttcttcttg tattgttaat
    12661 tagcattcta tatggaggtt tgatggaaat attgcaacag tttttccctc ctcgcaccag
    12721 cagttggtac gattttttgg caaatatact tggctgttta cttgcttttg gtagtattac
    12781 atggtttgtt aagaaaaaaa gaaatggata acaaacttct ataccaaata gccattactc
    12841 aaattaaagg attaggcagt ataaatagta aaaatttact cgcctatgtg ggtgatattg
    12901 aagaaatatt tcatcaaaaa tcgcatattt tacaaaaaat accgggtatt gggattctgt
    12961 taagcaaaag cattcttgaa caaaaagatg ctgcactcga aaaagccacc aaagaaattc
    13021 aatttattac caaacatggc attacacctc ttttctatac cgataagaat tatccagaac
    13081 gattaaaaga atgtccagat gctccaattc tgttgtatta taaaggaaat acaaatttaa
    13141 atacacaaaa aaccttagcc atagtaggca cacgcaatgc aactgaatat ggtaaaacaa
    13201 tgtgtactca acttattaaa gagttagcaa acaatcatcc tgaattgtgc atcattagtg
    13261 gattagctta tggcatagat atttgtgctc acaaagaagc cttacacatg cacttaccaa
    13321 ctgtagctgt attgggtcat ggtttagaca gaatttaccc tgctcagcat gctacaatag
    13381 ccaaaaagat acttacatca ggcggtttac ttacagaatt tccgagcgaa acaaatgcag
    13441 ataagcccaa ttttgtacgt cgaaatcgca ttatcgcagg tacaacagac gctacactta
    13501 ttgttgaaag tgctgcgcgt ggtggtgcta ttattactac cgaaatagct aattcatata
    13561 acagagatgt gtttgctgtg cctggtaaat gtactgatga atggtctgta ggttgcaata
    13621 acctaataaa acagcacaaa gcagcattag tgaatagtgc ttctgatatt gaatatatta
    13681 tgggttggac aaaagaacaa acaagccaaa aaatagaaca acaacaatct ttgtttatag
    13741 atttatccga tacagaacaa gaaattttta caattatcaa acaaaataaa gtcgttcata
    13801 t
//
LOCUS       DAOSRP010000048        13760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4542_length_13760_cov_5.753958, whole genome shotgun sequence.
ACCESSION   DAOSRP010000048 DAOSRP010000000
VERSION     DAOSRP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4542_length_13760_cov_5.753958"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1064)
                     /locus_tag="PLJ82_04345"
     CDS             complement(72..1064)
                     /locus_tag="PLJ82_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOR40769.1"
                     /translation="MNLYRLFCGKLTIKRLINYILLRLEYFISYSSKTPFIHAKPFAA
                     SIEPANYCNLQCPECPTGMGAIQKEATTLPFPLFKKMINPLLPELFYLNLYFQGEPFL
                     NESIYDMIAYANRQKIITNISTNGHFLTDSACQQLITSKLDRIIISLDGATEETYEKY
                     RVGGNFTAVVDGITRLVRLKKEMQSRRPFIELQFIVFSTNEHEIPAIKVLAKSLQVDG
                     LSIKTAQLTNFKKGNPLMPKNTIYSRYKLDKNGSYKRKKPLRNRCWKNWSSIVVSTDG
                     KVLPCCFDKHGLYAYGNLLDNGFHSLWNGDKATKFRSTILHNRKSLPMCNNCTE"
     gene            1290..2993
                     /locus_tag="PLJ82_04350"
     CDS             1290..2993
                     /locus_tag="PLJ82_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40770.1"
                     /translation="MKFTKTLFRFGAVVLLASVFASCSKMGGLPAEYITVNPNPLEVK
                     ANKVEAEITGTFPEKYFAKKAILEVTPVLRYEGGEAVGKTVIYQGEKVKDNNKVVAYK
                     TGGKFTQNISFDYVPGMKKSKLFLTFKAKIGKKEIQIPEVEIAQGTIVTATLANPLEI
                     AGATGADAFQRVIQEKYEAAILFQIQRAELQKKALTTAEVAALQNKISSIQANDSLKV
                     SSLGLSAYASPDGPADLNEKLAANRKKNTENFLTKELKKSKIDATVDAKYIAEDWDGF
                     QELMMQSDIQDKELILRVLSMYNDPAQREKEIKNLSAAYTKIADQILPQLRRSKLELV
                     VDVIGKSDEQILAIAKAEPSKLSLEELLYGASIATSASEKAYITGQAISLFPEDWRAY
                     NNLATLKFADGNVDEAKALLATAVAKGGDQPQVNFNLGLVELTAKNYKKAQEYFGKSA
                     GVGEALAEAQGVAYIQSGEYNKAVTAFGNSTSNNAALANLLAGNYQKATAVVDAVKNP
                     TADTYYLKALIGARSSNKDQVISSLTKAIQADKSYAKKAACDIEFAKYLIDSDFLAIV
                     K"
     gene            complement(3058..3966)
                     /locus_tag="PLJ82_04355"
     CDS             complement(3058..3966)
                     /locus_tag="PLJ82_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOR40771.1"
                     /translation="MYCILKLIKEWTLLIAIIFGALFHNFFGYIYSSFPFITPMLIFI
                     MLLLTFSKLEKLSLQIGKLHIKLLTVQLILSILVYAIGSIIHQTLAMGLLICVLVPTA
                     TSASVITNLLKVDIKFITSYLLFSNVVTAFIIPIFLAYAGTNSNENIFFSMYVLAKKV
                     LPTMLLPILIVFILKRYLPTMHQRLQHVSGITFYLWSFALTLVIGKTIHIFVTQETSS
                     YSTEIMLALGSLGICIIQFGLGRVIGKKHGERIASGQALGQKNTIFAIWLAQTYLSPL
                     VSIAPAAYVFWQNTINSYQIWKARRN"
     gene            complement(4046..4222)
                     /locus_tag="PLJ82_04360"
     CDS             complement(4046..4222)
                     /locus_tag="PLJ82_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40772.1"
                     /translation="MDTLIKNLGVIVIILGALALIIPSFLGFNSNTTLSIGGILLFAG
                     LVLHIIMTKKNTEK"
     gene            complement(4304..6178)
                     /locus_tag="PLJ82_04365"
     CDS             complement(4304..6178)
                     /locus_tag="PLJ82_04365"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOR40773.1"
                     /translation="MEEEIKNRHPEAEYTNDNIKTLEWQEHIRLRPGMYIGKLGDGSS
                     PDDGIYVLLKEVLDNSIDEFTMGNGKQIDVSIANGRVEVRDYGRGVPLESIVDVTSKM
                     NTGAKYDSKSFVKSVGLNGVGLKAVNALSSYFCIQSFRNGDTATAEFERGTIKTKSGV
                     VPNTVKKGNGTLILFEPDNTIFVGFVYQDEYIETLLKNYVYLNTGLTINYNGKKFYSK
                     NGLHDLLSDTITADTLYPIIHLKGEDIEIAFTHSNQYNDEYYSFVNGQHTTQGGTHLS
                     AFKEAITKTIKDFYNKGFESSDIRNGLVAAISVRVIEPVFESQTKTKLGSKDMEPNGV
                     SVYKYINDFIKKEVDNYLHKHAETAEILLRKIIDSEKERKAIAGVTKIARDRAKKANL
                     HNKKLRDCRIHYSDDKVGDKATESVIFITEGDSASGSITKSRDVNTQAVFSLKGKPLN
                     TFGLTKKIVYENEEFNLLQAALNIEDGIEGLRYNKIIIATDADVDGMHIRLLMISFFL
                     QFFPDLIKKGHVYILQTPLFRVRNKKETIYCYSEEERQEAVNKLGAKAEITRFKGLGE
                     ISPDEFKHFIGKDMRLDPVGLRKEDAVKELLAFYMGKNTPERQTFIIDNLIVEEDLEL
                     "
     gene            6361..7584
                     /locus_tag="PLJ82_04370"
     CDS             6361..7584
                     /locus_tag="PLJ82_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOR40774.1"
                     /translation="MKYLLIPPTALQGTVVLPTSKSISNRVLIINALAESDMPIEGVS
                     DCDDTRVMLNVLQSNDTAFDVGAAGTSMRFLTAFLSKIVGKWEITGSERMKNRPIKLL
                     VDALNELGAKIEYIEKEGYPPLRIYGSALQGGRIEMQGNVSSQYISALLMLAPTMQQG
                     LRLVLQGDIISVPYIQMTIKIMEQFGIQSYWKDNEIYIPNQAYTPVPFVVEGDWSAAS
                     YWYEMLALAPQGEVELKGLYKDSVQGDAAIATIFEHIGVKTKFTKQGVILSKKAPKKG
                     KLVYNFVNQPDLAQTCVVCCCLLGIPFYFSGLQSLKIKETDRIYALVTELKKLGYVVH
                     QKDNSILEFNNERCDVATEQSIDTYDDHRMAMAFAPACIKVGEISINEPHVVTKSYPN
                     FWVDLQSLGFTLHQM"
     gene            complement(7587..8678)
                     /locus_tag="PLJ82_04375"
     CDS             complement(7587..8678)
                     /locus_tag="PLJ82_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOR40775.1"
                     /translation="MSDSISLYFNDVLVNPTLPSYMSENEYIASNLHTQDFSVALIGI
                     PDKNNSANTVRNHLYTLFGNFTTIKLRDLGNLKKGKSEKEMALALEHVVQELAEKSIP
                     CIVFGGEQWLTTAIFKGITEVQPNVGVCFIDSKIDVGDMQESSCSHSFITQLATHHTL
                     DNIDIVGAQQYYYTERQRQYIREHKGDITRLSEARKDIFKLEPTLRNADLISIDMSAV
                     RLSDAPAAKFPCPNGFTGEEVCQLTRFAGYADKTKALVISAWDCINDHQHHTSRLVAE
                     TIWHFLDGVDKREQESPNEDNPIYTKLVVQSDIHDDGIIFYHNTQTDRWWIEIPTLSG
                     LRIYPCHTNDYQALCNNEIPAIWLRYLYK"
     gene            8862..9308
                     /gene="rpiB"
                     /locus_tag="PLJ82_04380"
     CDS             8862..9308
                     /gene="rpiB"
                     /locus_tag="PLJ82_04380"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOR40776.1"
                     /translation="MKALDTLYIGIASDHAGYEMKERLKEYFAHTFFALKDFGTHSEE
                     SVDYPDYIHPLAQAVAKNEIDFGIAICGSGNGVSIVANKHKNVRAALCWTDEIAILAR
                     KHNDANIISLPARFISQALAEDMVERFLTTDFEGGRHCNRVNKINE"
     gene            complement(9320..9784)
                     /locus_tag="PLJ82_04385"
     CDS             complement(9320..9784)
                     /locus_tag="PLJ82_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOR40777.1"
                     /translation="MAKEIERKFLVDKTKWQAVSKGMLLSQGYLSATENTTVRVRTTA
                     DKGFLTIKGKTQGFSRDEYEYIIPLQDAQEMLTMCSDKLVTKYRYTEKHNDMLWEIDV
                     FTDKNEGLIIAEIELTSEEQKFVLPDWVEKEVTGDIRYYNSFLAKTPFSTWE"
     gene            9858..11024
                     /locus_tag="PLJ82_04390"
     CDS             9858..11024
                     /locus_tag="PLJ82_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOR40778.1"
                     /translation="MMNPDSQFDDIRCYNDAEIPLQFSKLVEDKQLLAILPQILPNIP
                     VAVLIEQLKKVQSVEQFQKGFILPFLSDLETKTSKGVELIQLDRVNPHRHHLFISNHR
                     DIVLDSAFLNIHLLKNGYQTTEIAIGNNLLIYPWIEHLVRINKSFIVRRNASVREQIR
                     ISQELSAYIRYVITTKPHSVWLAQREGRAKDSDDRTQPALIKMLQMSAEGEYLKGIMD
                     LNIVPLSINYEHDPCDYLKAKEFQLKRDDECYKKSPADDLLNMSTGIMGYKGRISYVF
                     GTPLNDHPALNLVSQLSSKEQSTYIAHLIDTEIHRNYNLYPCNYVAADLLSKQNFFTK
                     HYSKEDEKDFIAYVQQRVNKIDISNKDEDFLTRKILEMYANPVYNQREAIRIQQ"
     gene            11036..11953
                     /gene="xerD"
                     /locus_tag="PLJ82_04395"
     CDS             11036..11953
                     /gene="xerD"
                     /locus_tag="PLJ82_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOR40779.1"
                     /translation="MIQNSNEHLVLRQYRSFLMLEKSFSDNTYQAYVHDVQKLLQFLE
                     DEKISYLTVTLVDLQHFLFQLAQIGINERSQARIISGIKSFYAFLVLENRLQDDPTSL
                     LESPKLPKKIPQILSIEEIDALIQAIDYSVPEGIRNRAIIETLYSCGLRVSELVELKL
                     SDIFWQEEFIKVVGKGNKERLIPISRTALKEIKSYLTERINLPIKKGSEDIVFLNRRG
                     NKLTRQMIFIIINKLADEIGLDKQIGPHTFRHSFATHLLEGGANLRVIQEMLGHESIL
                     TTEIYTHVDVHFLRETIMSYHPRNKKIKN"
     gene            12039..13034
                     /gene="pta"
                     /locus_tag="PLJ82_04400"
     CDS             12039..13034
                     /gene="pta"
                     /locus_tag="PLJ82_04400"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOR40780.1"
                     /translation="MKLLEKIFQQAKNNLRCIVLPESFEERTLRAADIVLQQQIAHIQ
                     LIGNKASILTTAKELGLAHIHQARFFDPETDPKMDEYSQLLYSLRKAKGMTLEEARVL
                     AKNPLYLSVLMIKNGDVDGELAGAANSTGNVLRPALQIIKTKPGVSVVSGVFFMLFDD
                     TSIGEDGVMVFADCAVTPNPSAIQLAEIAVVTAETTQIIGGFTPRVALLSFSTKGSAK
                     HEMVDKVVTATQLAQQQAPTICIDGELQVDAAIVPSVGASKAPNSLLKGNANVLIFPT
                     LETGNIAYKLVQRLAGAEAIGPVLQGMGAPINDLSRGCSVEDVVRMIAITANQAR"
     gene            13051..>13760
                     /locus_tag="PLJ82_04405"
     CDS             13051..>13760
                     /locus_tag="PLJ82_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOR40781.1"
                     /translation="MIVEKIEQFALSKKDHPKAFFSKIGIVGCGKEGSMLATLAAING
                     IEVVFMEPTEEKIHNAYARIESRLDERVDNWGLTPGEKRAILARVKGTVTYETFKGCD
                     FVIESIRYDDNTGERSTNLRREVFLNLENVLSKDAIIATNISTPIITELATELKHKDR
                     CIGVHVMSNVPDARILEVVKSLYTSDDVYTKVCHFAHLIKHEVILVAESAGLVSMRIF
                     FVMLNEACDMLMEGVSTV"
BASE COUNT         4536 a         2207 c         2593 g         4424 t
ORIGIN      
        1 caggctggta atggtgccat agcagatatt ctaatttcaa taaacaaaag ccgatgttca
       61 tcggcttttg tttattctgt gcaattgttg cacataggca atgattttct gttatgcaaa
      121 atggttgagc gaaattttgt tgccttatcg ccattccaca aggagtgaaa cccattatct
      181 aataagttgc cataagcata tagcccgtgt ttatcaaaac aacaaggaag cacttttccg
      241 tctgttgaaa ctacgataga actccaattt ttccaacatc gattacgcaa aggctttttc
      301 cgtttatagg agccgttttt gtccagttta tatctactgt atatagtatt ctttggcatt
      361 agtggattac cctttttaaa attagttaat tgagccgtct taatagataa gccatctacc
      421 tgtagtgatt ttgctaatac cttaattgct ggtatttcgt gttcattcgt actaaaaaca
      481 ataaactgaa gttcgatgaa aggtcttctt gattgcattt ctttttttaa acgcactagg
      541 cgtgtaattc catccacaac cgccgtaaaa ttaccaccca ccctgtattt ttcgtatgtt
      601 tcttctgttg ctccatcaag gcttataata attctatcaa gtttagatgt tatgagttgt
      661 tggcatgctg aatctgttaa aaaatgccca ttggtagata tattggtaat gattttttgc
      721 ctatttgcat acgcaatcat gtcataaata ctttcattca aaaaaggctc tccttgaaaa
      781 tatagattga ggtaaaatag ttcaggaaga agaggattaa tcatcttttt aaacaaagga
      841 aacggtagag tagtagcttc tttttgtatt gctcccatac ctgtagggca ttcaggacat
      901 tgtaaattgc agtaatttgc tggttctata gatgcggcaa aaggttttgc atgtataaaa
      961 ggcgttttac tactatacga gataaaatat tctaaacgta aaagaatata gttaattaga
     1021 cgttttatcg taagcttacc acaaaaaagc cgatataaat tcatatccct cattatttac
     1081 agcaaaagtc agcattatta tgtagaacag caaagatttt gtattaaata tctttgagat
     1141 gaggttaaaa ataccaaaaa tagaattaga aagactccat tgagttttac aacaaaattc
     1201 tttaaacaaa aagttgttat tattttttat attatgtatc tttgccctaa ttctgtaaaa
     1261 tgaatgtata acccaattaa ttgataagta tgaaattcac aaaaacatta tttcgttttg
     1321 gagctgtagt attactggct tccgtatttg catcatgtag taagatgggc ggattacccg
     1381 ctgagtatat cacagttaac cccaatcccc tcgaagtaaa agcaaataaa gtagaggcag
     1441 aaattacagg aacttttcct gaaaaatatt ttgccaaaaa agctattttg gaagttacac
     1501 cagtattgcg ttacgaaggt ggtgaagctg taggtaaaac agtaatttac caaggagaaa
     1561 aagtaaaaga taacaacaaa gttgtggcat acaaaacagg tggaaagttt actcaaaaca
     1621 tttcgtttga ttatgtgcca ggaatgaaaa agtctaaatt atttttaact ttcaaagcaa
     1681 aaataggtaa aaaagaaatt caaattccag aagttgagat tgcacaaggt accattgtaa
     1741 cagctacatt agcaaatcca ttagaaattg ctggagctac aggtgctgat gcattccaac
     1801 gtgttattca agaaaaatac gaagccgcta ttttattcca aattcaacgt gctgaattgc
     1861 aaaaaaaggc acttactaca gcagaagttg ctgctttgca aaacaagatt tcttctattc
     1921 aagctaatga cagtttgaaa gtatcttctt taggtttatc tgcttatgct tcgccagacg
     1981 gtccagctga cttgaacgaa aaattggctg ctaatcgtaa aaagaataca gaaaactttt
     2041 tgacaaaaga attgaaaaag tcaaaaatag acgctacagt tgatgcaaaa tatattgcag
     2101 aagactggga tggatttcaa gagttgatga tgcagtctga tattcaagat aaagaattaa
     2161 tcttacgtgt attatctatg tataacgacc cagcacaaag agaaaaagaa atcaaaaatc
     2221 tatctgctgc ttatacaaaa attgctgatc aaattttgcc acaattacgt cgttcaaaat
     2281 tagaattagt agtagatgtt attggtaaat cagacgaaca gattttggca attgccaaag
     2341 cagagccttc aaaattatct cttgaggaat tgttgtacgg agcgtcaatt gctacttctg
     2401 cctctgagaa agcatatatt acaggacaag caatttcttt gttcccagaa gattggagag
     2461 catacaataa tcttgcaact ttgaaatttg ctgatggtaa tgttgatgaa gcgaaagcct
     2521 tattagcaac tgctgtggca aaaggtggcg accaaccaca agttaatttc aatttaggat
     2581 tagtagaatt gacagcaaag aattacaaaa aagcacaaga atattttggt aaatcggctg
     2641 gtgtaggcga agctttagca gaagcacaag gtgtggctta cattcaatca ggagagtata
     2701 acaaagcggt aacagcattt ggtaattcta cttctaacaa tgctgcattg gctaatttat
     2761 tagcaggtaa ttatcaaaaa gcgacagctg tagttgatgc agttaaaaat ccaactgcgg
     2821 atacatatta tttaaaagca cttattggtg ctcgttctag taataaagat caagtaataa
     2881 gttcattaac aaaagctatt caagcagata aatcgtatgc taagaaagca gcttgtgata
     2941 ttgaatttgc taaatattta attgactctg actttttggc tattgtaaag taaattttct
     3001 agttagatga tcataaaaaa gaggctatcc tttttggata gcctcttttt atatgattca
     3061 gttgcgtcga gctttccaaa tttgatacga attaatggta ttttgccaaa aaacatatgc
     3121 tgctggagca atagatacta atggcgatag gtatgtttga gcaagccaaa tggcaaaaat
     3181 agtattcttt tgtccaagag cctgaccaga ggcaatgcgt tctccgtgtt tttttccgat
     3241 aactctacca agcccaaatt gtattataca tatacctaat gagcccaaag ctagcattat
     3301 ttctgtagaa taggatgagg tttcttgtgt aacaaaaata tgaatagttt tgccaattac
     3361 caacgttaat gcaaacgacc ataagtaaaa agtaatacca cttacatgct gtaagcgttg
     3421 atgcatagtg ggcaaatagc gttttagaat aaacacaatt agtataggaa ggagcatggt
     3481 aggtaatact tttttggcta atacatacat ggagaaaaaa atattttcgt tagagttagt
     3541 tcctgcatat gccaaaaata taggtataat aaaagccgta actacattgc taaataataa
     3601 atagctagta ataaatttga tatctacttt caataaattg gtaattacag aagccgatgt
     3661 tgctgtaggt actaatacgc atataagtaa tcccatagcc aatgtttgat gtattatact
     3721 accaatagca tacacaagaa tactaagaat gagttgtaca gtgagtagtt tgatatgcaa
     3781 tttaccaatc tgtaaactta atttttctaa tttagagaaa gtaagcaata gcatgataaa
     3841 aataagcata ggagtaataa atggaaagga ggaatatata tatccaaaga aattatgaaa
     3901 caaagctcca aaaattattg ctatgagcaa tgtccattct tttattaatt ttaaaataca
     3961 atacatatga ttaaataaaa aacctttcct tatataatct ggaaaggttt agtgtacata
     4021 agaaaatagt tagacagata gttatttact tttcagtatt tttttttgtc atgattatat
     4081 gaaggactaa accagcaaat aataagattc caccaatact aagtgttgtg ttggaattaa
     4141 atccaaggaa agaaggaata atcagagcta aagctcctaa aataataacg ataactccta
     4201 ggtttttaat taatgtatcc ataatgtgtt tttttgtggt atgttttgtt acgcaaataa
     4261 acgaataatt cttgttgtat aaaaatattt ttcaaaaaaa atgctacaat tctaaatctt
     4321 cttctactat gaggttgtca ataataaaag tctgtctttc aggagtgttt ttgcccatat
     4381 aaaaagctaa aagctccttc acagcatctt cttttctaag cccaacaggg tctaaacgca
     4441 tgtccttacc aataaaatgt ttaaattcat caggcgatat ctctcccaat cctttaaatc
     4501 gagtaatttc agctttagct cctaatttat ttactgcctc ttgtctttct tcttctgaat
     4561 aacaatagat cgtttctttt ttatttcgga ctctaaaaag cggtgtttgc aagatgtata
     4621 cgtgcccttt tttaatcaaa tcgggaaaga attgcaaaaa gaaactaatc attaatagtc
     4681 gaatgtgcat accatctacg tcggcatctg ttgcaatgat aattttattg tatcgcaatc
     4741 cttcaatacc atcttcaata ttaagagcag cttgcaagag gttaaactct tcattttcgt
     4801 acacaatttt tttagttaaa ccaaaggtat ttaatggctt tccttttaaa ctaaaaacgg
     4861 cttgcgtatt tacatcgcga cttttagtaa tagagccact agcagaatct ccttcggtaa
     4921 taaaaattac agattctgtt gctttatcac caactttatc gtctgaataa tgaatacgac
     4981 aatcacgtaa ttttttgtta tgtaaattgg ctttttttgc tctgtctcta gctattttag
     5041 taacacctgc aattgctttg cgttctttct ctgaatcaat aattttcctg agtaaaattt
     5101 cagcagtttc tgcgtgttta tgcaaataat tgtcaacttc tttctttata aaatcgttga
     5161 tgtatttata taccgacaca ccgttcggtt ccatatcttt agaacctaac ttggttttag
     5221 tttgtgattc aaacacaggt tctattacac gcacactgat agctgccact aagccattgc
     5281 gtatatctga tgattcaaag cctttattat aaaagtcttt tatcgttttg gtgatagctt
     5341 ctttaaaagc tgaaaggtgt gtgcctcctt gtgtggtatg ttgtccgttt acaaacgaat
     5401 aatactcatc attatattga ttgctgtgtg taaatgcaat ttcgatatct tctcctttga
     5461 ggtgaataat aggataaagc gtatcagcag taatggtatc gcttaataaa tcgtgcaatc
     5521 catttttact ataaaacttt ttaccattat aattgatggt aagccctgtg tttaaataaa
     5581 catagttttt taataaggtt tctatgtatt catcttggta tacaaaacct acaaaaattg
     5641 tattatctgg ctcaaaaaga ataagagttc cattgccctt ttttacggtg ttgggtacaa
     5701 ctcctgattt ggttttaatt gtgccacgct caaattctgc cgttgcggta tctccatttc
     5761 taaaactttg tatgcaaaaa tacgaagaca atgcatttac cgcttttaga cctacaccgt
     5821 ttaaccccac agatttaaca aaagacttag agtcgtattt agctcctgta ttcattttgg
     5881 aggttacatc aacaatactt tctaagggaa ctccacgtcc gtaatctctt acttcaaccc
     5941 gtccatttgc tatggaaaca tcaatctgtt tcccattacc catagtaaat tcgtctatcg
     6001 agttgtctaa tacctccttg agtagcacat atataccatc atcaggcgag gagccatctc
     6061 ctagcttacc aatatacatg ccagggcgta agcgtatatg ctcctgccat tccaacgttt
     6121 tgatgttatc gttggtgtat tcagcttcgg gatgtctatt tttaatttct tcttccataa
     6181 ttttaacgta caaaggtaca tttttttaaa taaaaaggaa agatttagat atgggaaaag
     6241 atgtactttt ttaaaaaaaa taatgggtta aagaaatatg cttgaaaatg tgtatttttg
     6301 caatttgaaa gatagtataa acgctttcat ccatactaaa caaaacatag gaataaaaga
     6361 atgaaatatt tgttaatccc gcccactgcg ttacaaggta cagtcgtatt gccaacatct
     6421 aaaagtataa gtaatagagt gttgattatt aatgctttgg cagaaagcga tatgcctatt
     6481 gaaggtgttt ctgattgcga cgacacgcgt gtgatgttaa acgtattgca atcaaacgac
     6541 accgcatttg atgtaggagc agcaggcacg tctatgcgtt ttttaacagc atttctatct
     6601 aagatagtag gtaaatggga aataactgga agcgaacgca tgaaaaacag acccattaag
     6661 ctattggttg atgcactgaa tgagttagga gcaaaaattg aatacattga gaaagaggga
     6721 tatccaccgt tgcgtattta tggcagtgca ttgcagggtg gtcgtattga aatgcaaggg
     6781 aatgttagta gtcagtatat ttctgctttg ttaatgttag cacctactat gcaacaagga
     6841 ttgcggttag tgttgcaagg cgatattatt tcggtgccat acatacaaat gacgataaaa
     6901 ataatggagc agtttggtat tcaatcatac tggaaagaca atgaaatata cattcctaac
     6961 caagcttata caccagttcc cttcgtggta gaaggagatt ggtcggctgc ttcgtattgg
     7021 tacgaaatgc ttgcattagc accacaagga gaggtagaat tgaaaggctt gtataaagat
     7081 agtgtgcagg gtgatgctgc tattgctaca atttttgaac acattggtgt gaaaacaaag
     7141 tttacaaagc aaggagtaat cttatctaag aaagctccaa aaaagggcaa actagtatat
     7201 aattttgtaa atcagcctga tttggctcaa acctgtgttg tgtgttgctg tttgttaggg
     7261 ataccttttt atttttcagg tttacaaagt ttaaaaatta aagaaacaga cagaatctat
     7321 gccttggtta cggaattaaa aaagctagga tatgtcgttc atcaaaaaga caatagtatt
     7381 ttagagttta ataatgaacg gtgtgatgtg gctactgaac agagcataga tacatatgat
     7441 gatcatcgta tggctatggc atttgctcct gcttgtataa aagtaggaga aatttctatc
     7501 aacgaaccac atgtggtaac gaaatcttat ccaaatttct gggttgattt gcaatcgctt
     7561 gggtttactt tgcaccaaat gtaggcttat ttatatagat aacgaagcca tatcgctggt
     7621 atttcgttgt tgcataaagc ctgataatcg tttgtatgac atggatagat acgtaatcca
     7681 gaaagggtag gtatttcaat ccaccaacgg tcggtttgtg tgttgtggta aaaaataata
     7741 ccgtcatcgt gaatgtctga ttgtaccaca agttttgtat atatgggatt atcttcgttt
     7801 ggagactctt gttcacgttt gtctacgcca tccaagaaat gccatattgt ttcagcaact
     7861 aaacgtgacg tatgatgttg gtgatcgttg atgcagtccc atgctgaaat aacaagagcc
     7921 ttcgttttgt cagcatagcc agcaaaacgt gttagttggc aaacttcttc acctgtaaat
     7981 ccgttgggac aaggaaactt agccgctggt gcgtcagata gacgtacagc actcatatca
     8041 atacttatta aatcggcatt gcgtagagtt ggctcgagtt tgaaaatatc tttacgagct
     8101 tctgataagc gggttatatc tcctttatgt tctcgaatat actgtctttg tctttctgtg
     8161 taataatatt gttgggctcc tacaatgtcg atattatcta gtgtgtgatg tgttgcaagt
     8221 tgtgtgataa aagaatgaga acagctcgat tcttgcatat cgccaacatc aattttggag
     8281 tcgataaagc acacccctac attgggttgc acctcagtta ttcctttaaa aatagcagtt
     8341 gtgagccatt gttctccgcc aaacacaata caaggaatag atttttctgc caactcttgt
     8401 actacatgct ccaatgccaa tgccatttct ttttctgatt ttcctttctt cagattgccc
     8461 aaatcacgta gttttatagt agtgaagttg ccaaaaagtg tgtacagatg gttgcggacg
     8521 gtattggcag agttattttt atcaggtatg ccaataagtg ctaccgaaaa gtcttgcgtg
     8581 tgaaggtttg atgcaatgta ttcattttct gacatatacg atggtaatgt tgggtttacc
     8641 aatacgtcgt taaaatataa ggaaatagag tcggacatgc ttgtaaaaaa ttagagtaaa
     8701 tttagtcaat tgttgtgtgt aaaaaatgta tctttgcaga gcaaacaaaa atacatattt
     8761 tttgcaaact gaacaacaat atgattcctc atagtaaaat atggttgggt tattcatgtt
     8821 tttttaaata gataattgta tcgtcaaaca atcgaacaaa aatgaaagca ttagacacct
     8881 tgtatatagg tattgctagc gaccacgctg gctatgagat gaaagaaaga ttgaaagagt
     8941 attttgcaca cacgtttttt gctcttaaag attttggtac tcattccgaa gagagtgttg
     9001 attatcctga ctatatacac ccattagcac aagcagtagc taaaaacgaa attgattttg
     9061 gtattgctat ttgtggtagt ggcaatggag taagtattgt tgccaacaaa cataaaaatg
     9121 tacgtgcagc actttgttgg acagatgaaa ttgccatatt agctcgcaag cataatgatg
     9181 cgaacattat atcattgcca gcacgtttta tttcgcaagc tttggcggag gatatggtag
     9241 agcgttttct gactactgat tttgaaggag gcagacactg taaccgagta aataaaatta
     9301 atgaataagg aaattgatgt tattcccaag tagaaaatgg cgttttagcc aaaaatgagt
     9361 tgtaatagcg gatgtctccc gttacctctt tttctaccca gtcgggcaat acgaattttt
     9421 gttcttcact tgtaagttct atctcagcta taattaatcc ttcgttttta tctgtaaaaa
     9481 catctatttc ccaaagcata tcattgtgtt tctctgtgta acggtacttt gttactagtt
     9541 tgtcagagca catcgttagc atttcttgtg catcttgaag cgggattatg tattcgtatt
     9601 catctcgact aaacccttgc gtttttcctt tgatagttaa aaaacctttg tccgctgttg
     9661 tgcgtacacg gacagtagtg ttttcggttg cagacaaata tccctgagaa agcaacattc
     9721 cttttgatac agcttgccat tttgttttat ccaccaaaaa ttttctttca atttcttttg
     9781 ccatagtcgt atttattgca tacaaatgta cgtgtttttc taaaaatttg tatctttggg
     9841 caaaagaaaa taatactatg atgaatcctg atagtcagtt tgatgatatt cgttgttata
     9901 acgatgcaga aatacccttg caatttagca aattagtaga agacaaacag ttgcttgcca
     9961 tactaccaca gattttgccg aatattccag ttgcggttct tatcgaacag ctaaaaaaag
    10021 tacaatctgt agagcaattt caaaagggat ttattttacc ctttttatct gatttagaaa
    10081 cgaaaacatc aaaaggagta gagttgattc agttagatag agtaaatcct cacagacatc
    10141 atttatttat atccaatcac cgagatattg tgcttgattc agcattttta aatattcatt
    10201 tactaaaaaa tggatatcaa acgacagaga ttgccattgg aaataattta cttatttacc
    10261 cttggataga acatcttgtt aggattaaca aaagttttat tgttagacgc aatgcctctg
    10321 tacgcgaaca gatacgtatt tcgcaagaat tatctgctta tattcggtat gtaattacca
    10381 caaaaccaca ttctgtatgg ctagcacagc gagagggaag agctaaggat tcggatgata
    10441 gaacacaacc agcattgatt aagatgctac aaatgagtgc agagggagag tatctaaaag
    10501 ggataatgga tttgaatata gtgccattgt caatcaatta tgaacacgac ccttgtgatt
    10561 atttaaaagc caaagaattt cagcttaaac gagatgatga atgttataaa aaatcacctg
    10621 ctgatgattt gctgaatatg tctactggta ttatgggcta taaaggacgt atttcgtatg
    10681 tgtttggaac tcctttaaat gatcatcctg cgttgaactt agtctctcaa ctttcgtcaa
    10741 aagagcaatc tacgtatata gcacacttga tagatacaga gattcataga aattacaatt
    10801 tgtatccgtg taattatgtg gctgctgatt tattgagtaa gcaaaatttc tttactaagc
    10861 attattccaa ggaagatgaa aaagatttta ttgcgtatgt gcaacaacga gtgaataaaa
    10921 ttgatatttc gaataaagat gaggattttt taacccgaaa aatactcgaa atgtatgcca
    10981 atcctgttta taatcaacga gaagccattc gaatacaaca ataaaaaata cttatgtgat
    11041 tcagaatagt aatgaacatc ttgtattacg tcagtatagg tcttttttaa tgcttgaaaa
    11101 gtctttttct gataatacct atcaggcata tgtgcatgat gtgcaaaaat tattgcaatt
    11161 tttggaggac gaaaaaatct cctatttaac tgttactttg gtagatttac agcacttttt
    11221 atttcagttg gcacaaattg gtatcaacga gcgttcgcag gctcgtatta tatcggggat
    11281 aaaatctttt tatgcttttt tggtgctcga aaatagatta caggatgacc caacgagttt
    11341 gcttgaatca ccgaaattac ccaaaaaaat accgcaaatt ttatcaatag aagaaattga
    11401 tgcgttgatt caagccattg attattctgt gccagagggg ataagaaaca gggctataat
    11461 tgaaacattg tatagttgtg ggttgcgagt gtctgaattg gtggagttga aactttctga
    11521 tattttttgg caagaagaat ttataaaagt ggtaggaaaa ggcaataagg aacgattgat
    11581 accgatatct cgtacggcat tgaaagaaat taaaagttat ttaacagaac gtatcaattt
    11641 gcctataaaa aaagggagtg aagatattgt ttttttaaac agaagaggga ataaactaac
    11701 gcgacaaatg atttttatca tcattaataa attagcagat gaaataggct tagacaaaca
    11761 aattggtcca catacatttc ggcattcttt tgctactcat ttactcgaag gaggagctaa
    11821 tttgagggtt attcaagaaa tgttagggca tgaaagtata ttaacaaccg aaatttatac
    11881 gcatgtagat gtgcattttt tgagagaaac gataatgtca tatcacccta gaaataaaaa
    11941 aataaaaaac taaaaaatgc ttgtttcaat gctattattc ttgtaacttt ggtacttcta
    12001 tttaaaaaaa acaatttatt attcctaagg tgttttatat gaagttatta gaaaaaatat
    12061 ttcaacaagc aaaaaacaat ttacgttgca ttgtattgcc cgaatcattt gaagaacgca
    12121 cattgcgtgc cgcagatatt gtcttgcaac aacaaattgc acatatccag ttaattggta
    12181 ataaagctag tattcttaca acagccaaag agctgggttt agcccatatt caccaagcga
    12241 gattttttga tcccgaaaca gaccccaaga tggacgaata tagtcagctt ttgtattcgt
    12301 tacgtaaggc aaaagggatg acgttagaag aagctcgggt attagcaaag aatcctttgt
    12361 acctctcggt gttgatgata aaaaatggag atgtagatgg agaattggct ggtgccgcaa
    12421 attcaacggg aaatgtattg cgaccagcat tacaaattat aaaaactaaa ccaggggtaa
    12481 gtgttgtatc tggcgtattt tttatgctat ttgatgacac aagcataggt gaagacggtg
    12541 tgatggtgtt tgccgattgt gccgttactc ctaatccctc ggctatacaa ttagcagaaa
    12601 tagctgttgt aacggcagaa acaactcaaa tcatcggagg gtttactccg cgtgttgctt
    12661 tgttgagttt ttcaaccaaa ggaagtgcca aacatgaaat ggtagacaaa gtagtaactg
    12721 ctacacaatt agctcaacag caagctccaa caatatgtat agatggagag ttgcaggtag
    12781 atgctgccat agttccgtcg gttggggctt ctaaagcacc caacagtttg ttaaaaggga
    12841 atgcaaatgt attgatattc cctacattgg aaactggaaa tattgcatat aaattagtgc
    12901 aacgtttagc tggggcagaa gctattggtc cagtgcttca aggaatgggt gctccaatta
    12961 atgatttgtc aagaggttgc tcagtagagg atgttgtgcg gatgattgct attacagcaa
    13021 atcaggctag ataatttaag gataagaaat atgatagttg aaaaaattga acaatttgcc
    13081 ctaagtaaga aagatcatcc caaagctttc ttttcaaaaa tcggaattgt aggttgtggt
    13141 aaagagggaa gtatgttggc gacattggca gccattaatg ggatagaagt agtgtttatg
    13201 gaacctacgg aagaaaaaat ccataatgct tatgcacgca tcgaaagccg attagacgaa
    13261 cgcgttgata attgggggct tacgccaggt gagaaaagag caatactcgc tcgtgtaaaa
    13321 ggaacagtaa cgtacgaaac gtttaaaggt tgtgattttg taattgaatc tattcggtat
    13381 gatgataata caggagaaag aagtacaaat ttgagacgag aagttttctt gaacttagaa
    13441 aatgtgttgt ctaaagatgc tattatcgcc accaatatat ctactccaat tattacagaa
    13501 ttagctaccg aactcaaaca taaagataga tgcataggag tgcatgtaat gagcaatgtg
    13561 ccagatgctc gtattttgga ggtggtaaaa agtttatata ccagtgatga tgtatatacc
    13621 aaagtatgtc attttgctca tttaattaaa catgaagtaa ttttggttgc tgaatcagca
    13681 gggttagtga gtatgcgcat tttctttgtt atgctcaatg aagcttgtga tatgttgatg
    13741 gaaggagtgt ctaccgtaga
//
LOCUS       DAOSRP010000049        13709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4560_length_13709_cov_5.041966, whole genome shotgun sequence.
ACCESSION   DAOSRP010000049 DAOSRP010000000
VERSION     DAOSRP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4560_length_13709_cov_5.041966"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLJ82_04410"
     CDS             complement(<1..222)
                     /locus_tag="PLJ82_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HOR40782.1"
                     /translation="MKLTFTIQYHTSWGQQVCILGSLPALGNNIPTKALKMNYIENGQ
                     WEASIQVRSLASFSYTYVVVDANGHTISEW"
     gene            complement(354..2333)
                     /locus_tag="PLJ82_04415"
     CDS             complement(354..2333)
                     /locus_tag="PLJ82_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOR40783.1"
                     /translation="MQNTTNTFDYHVDKFADIEILRYRVTGFEALSLQQKQLIYHLSE
                     AAQEGRDILFDQHYEHNLCIRRTLEAIYETHNETEKNVEEFNAFEIYLKRIWFSNGIH
                     HHYSTDKLEITCSKEYFTSLLKKVPVSLLPLEKNETIDSFYTKIITLIYNPEIDKKRV
                     NLADNQDLITSSANHFYKNVTQQEAEDFYNGLQDSSDLQPISYGLNSCLVKENGIIKE
                     KTWKLNGMYTAAIEKIIYHLSNAMAFAENSKQKLIIEKLIAFYQTGNLQTFDEYSILW
                     VKDTSSEIDFLNGFIETYGDPLGRKGSWEAIVNFINKEATQRTQTISNHAQWFEKHSP
                     VNEKFKKTSVVGVSAKVITNAMLGGDSYPATPIGINLPNSNWIREVHGSKSVTIDNMM
                     EAYHHAASGNGFNEEFMWSQHECELTEKYGYITDKLHTDLHECVGHGSGKMLDGVSPE
                     ALKAYGATIEETRADLFGLYFIADPMMVEIGLLPNMDAYKAEYYRFMLNGLMTQLVRI
                     KLGDTIEESHMRNRQLIANWVFEKGQKQKCIEKRVQNNKTYIIIHDYVALRTLFGELL
                     AEIQRIKSEGDYTAAKYLVEKYAIQIDYSLHKEILERYTALDIAPYKGFVNPTYEQVY
                     NEYGEVIDIVPHYTESYTEQMLRYSKTKSYLPTYN"
     gene            complement(2341..3162)
                     /locus_tag="PLJ82_04420"
     CDS             complement(2341..3162)
                     /locus_tag="PLJ82_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40784.1"
                     /translation="MKKIALLILFTLLCVSHVVYAQTIQENEQMIVSWFEDIKETQSD
                     SAKIQLNKKIEQLLYTTLQEPNAFNYSFPALKYIGSIYAPDKLMRVFSWNYPLSNGKY
                     GYSALFMFKNKPKSTPSLHLVSTTTAFKPEETRKYDPSTWYGALYYNVIAHKISRDKT
                     EYLLLGISNYQPITKVKIIESIKISNRQLTFGTPLFKKDKKTLYRAVFEYKKEVNMLL
                     EYESSKKRFVFDHLSPSDPIYKDVYHFYGPDFSYDSYQWKKKKLHYTPDIDVRNK"
     gene            complement(3235..5064)
                     /locus_tag="PLJ82_04425"
     CDS             complement(3235..5064)
                     /locus_tag="PLJ82_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOR40785.1"
                     /translation="MYNDIPLSSFLPTTQKEIKALGWEELDVILFSGDAYIDHPSFGI
                     AVMGRILEQQGLKVAIVPQPNWRDDLRDFKKLGKPRLFFGVSAGSMDSMVNHYTANKR
                     LRSNDAYTPEGRAGYRPDYATVTYCTILKSLYPDTPIVIGGVEASLRRVTHYDYWSDK
                     LLPSILVDSQADLLVYGMGEKAIVELVQLLQQNIPFQQLHDIPQTAFLIDKTANSQST
                     GEWESLSLFSHEECIASKINQAKNFRVIEEHSNSQKAGRLLQDVGTKTVIINPPYPTM
                     TEKEIDQTYTLPYTRLPHPRYKGKTIPAYEMIKHSVTLHRGCFGGCAFCTISAHQGKF
                     IASRSEKSILAEIEKITQMPDFKGYISDIGGPSANMYKMQGIDLTICEKCKKPSCIHP
                     KICKNLDTNHAPLIELYKKVRSIKEIKKAFVGSGIRYDMILHKTGNKQTDASNMEYAN
                     ELIAHHVSGRLKVAPEHTNDEVLNLMRKPSFEYFETFVKLFNEVNKQHHLNQQLIPYF
                     ISSHPGCKQQHMAELAAKTKALDFKLEQVQDFTPTPMTLATEMYYTGIHPYTLEKIST
                     PNTPQQKLNQRKFFFWYQKEYKNEIINDLKKMNRMDLIKKLYP"
     gene            complement(5087..6721)
                     /locus_tag="PLJ82_04430"
     CDS             complement(5087..6721)
                     /locus_tag="PLJ82_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOR40786.1"
                     /translation="MKKIFFVFITIVSFQYVLAQASEKKNFEIAKNVDIFNSIVKQLQ
                     VYYVDSIPMEEIVSSGIDAMLTKLDPYTNYIPESDTKDFLQMTTGEYGGIGSVISLRN
                     SQIHIVELYENAPAHTYGLQVGDKILEVDGKSTKGLSVSEVSSMLKGTPSTTVQIVVE
                     RMGMKKPIHYSIVREKIYLNPVTYAGILPGKSNIAYIRLNSFTENCAEEVKKALQDLA
                     KKGATSLILDLRGNGGGVINDAIAISGLFLPKGTEIVSTKGKNAEMDRSYKTSSDPLF
                     PNLPLAILVNESSASAAEIVAGAMQDLDRGIIIGRNTYGKGVIQSTYPLPYNTNIKIT
                     IAKYYTPSGRCIQSSKHIARLASTNSDSIEHVFATQNGRVVKEGQGIMPDILVPESTL
                     KTITYHLVSNAYFYDFVNIYTQQHATIPPIEEFAVSDDLFNQYKDYIIKQNFTYELQS
                     EKGLQKLIETAKNEGYYDENKVYFSDLEKVLKHDINQDIIDAKEEISELLAAEIVKRY
                     YFQRGELQQLLKNDKELKRAIEIVSDQDTYQTMLKP"
     gene            complement(6862..6935)
                     /locus_tag="PLJ82_04435"
     tRNA            complement(6862..6935)
                     /locus_tag="PLJ82_04435"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6899..6901),aa:Asn,seq:gtt)
     gene            complement(6954..7027)
                     /locus_tag="PLJ82_04440"
     tRNA            complement(6954..7027)
                     /locus_tag="PLJ82_04440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6991..6993),aa:Asn,seq:gtt)
     gene            complement(7227..9773)
                     /locus_tag="PLJ82_04445"
     CDS             complement(7227..9773)
                     /locus_tag="PLJ82_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOR40787.1"
                     /translation="MENEMNYSDNVADILRYSHEEAERLGNNYIGPEHLLLAIIRAGE
                     GKAFDVLLSLNLDLLRIKKTIESSIRTSENVMIGSNIPLLKSTEKILKFIVLEARQFG
                     NYMADSEHLLLAILKDKTNLVTEIFSVENITYDTVRALVEKKDIGSIDKKNGVNSNDE
                     DGEDEDSEFSSNSVEKNGKTSTFSTSTKYTKNTSKTPALDNFGTDITKAAEEGKLDPI
                     VGREKEIERLSQILSRRKKNNPILIGDPGVGKSAIVEGLALRIIQRKVSRILFGKRVV
                     SLDMASIVAGTKYRGQFEERLKAILNELETNPDVILFIDEIHTLIGAGATAGSLDAAN
                     MMKPALARGLIQCIGATTLDEYRQSIEKDGALERRFQKVIVKPSTKEETLAILQNIKE
                     RYEDHHHVTYTPEALEACVYLTDRYISDRSFPDKAIDAMDEAGSRAHLHNVSIPSNIE
                     ILESEIDEANKSKLQAVKDEDFRLANEYKDRIKELNILLKNAKQEWEQEIKNQHVTVD
                     ANSISEVVSMISGVPVQQIAEGEFDKLRRMSAILKEKVIGQDTAIEKISKSILRNRVG
                     LKDPNKPIGSFIFLGPTGVGKTYLAKILAEFLFNSKDAIIRIDMSEYMEKFSVSRLIG
                     APPGYVGYEEGGQLTEKVRRNPYSIVLLDEIEKAHPEVFNLLLQVLDEGSLTDSLGRK
                     VDFKNTIIIMTSNIGTRQLKDFGRGVGFSTTSRLDDTEFAHSVIKKALNKSFAPEFLN
                     RVDDVIIFEQLSKESILKIIDIELKQVVERIESLGYTLQLTEAAKNHIANEGYDVQYG
                     ARPLKRTIQKYVEDEIAELIINNTIENAKNTPIKIDYNEDIKKISSGILV"
     gene            10029..12638
                     /gene="gyrA"
                     /locus_tag="PLJ82_04450"
     CDS             10029..12638
                     /gene="gyrA"
                     /locus_tag="PLJ82_04450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR40788.1"
                     /translation="MIDQERIIKINIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMNELGSNSNKPYKKSARIVGEVMGKYHPHGDSSIYGTLVRMAQEWSMRYQLV
                     DGQGNFGSIDADEPAAMRYTEARMHRLAEEMLMDIDKDTVDFKLNFDDTLKEPTVLPT
                     RIPNLLINGASGIAVGMATNMAPHNLSDTIDAIVAYIDNKEVDDEELIKLIKAPDFPT
                     GGIIYGYAGIRDAYLTGKGRVVIRGRVTIEVEKDRERIVITEIPYLVNKAELTKHISD
                     LVNEKRIEGISNISDESNYEGIRLVVEIKRDANANVVLNNLYKQTSLQTSFSVNNIAL
                     VKGRPRLLVLRDLIELFVEHRHEVVVRRTQFELKKAQERAHIVEGLVIASDNIDEVIA
                     IIKAASTPNDAMEKLMVRFGLSELQVRAIVDMRLSQLTNIQQDKLRQEYQDLLVLIAK
                     LEDILGNETLRMQIIKDELIEVKDKYGDERRSEIVYASEDFNPEDFYADEEMIITISH
                     LGYIKRTPLSEYKLQNRGGVGSKGGESRDEDFIEYIYPASMHNYMLFFTQKGRCYWLK
                     VYDIPEGSKISKGRAIQNLLNIESDDKVNAFIRIKQLQDPEYNQSHYLLFCTKQGVIK
                     KTRLDAYSRPRQNGVNAITIREDDDVIQVRLTDGDSEVVIANKNGRAIRFHESKVREM
                     GRTATGVRGITLDEDGQDEVIGMITIPKEEKTKESILVVSEKGYGKRTLIDEIDENGN
                     AIVDENGDPVPVYRITNRGGKGVKTMNITDKTGKLVAIKNVSDNNDLVIINKSGIAIR
                     LEVASMRIMGRATQGVRLINLEKRNDEIASVCKVVSEEKENLDEVSPELLDAENNFNS
                     IEGIATNDETNNE"
     gene            12658..>13709
                     /locus_tag="PLJ82_04455"
     CDS             12658..>13709
                     /locus_tag="PLJ82_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40789.1"
                     /translation="MKKTLWMTLGLLLFTIATGCAQKSQVNKAYNRLFTEPIDYAAAT
                     EDIEVAKKDSTTATQSRTWYVAGRIGYTMANSEVTKMRMQQPANDENLYQGLKQMYEN
                     YVVADKFDGVVDKKGRIKYSQRRNIKADFKEMHPFYINAGATMFEYKEFAKAYTLFNQ
                     YIKIADLAIWEEKDAIKIDSTYNTIQFYAGIVASNMDSTQLAIKHFKNLIGTSYPQKE
                     AVYEMLYSLYQHANDTVNFISILKEGVELYPESPFFIGNLINYYATASKFTEALDYLN
                     EALERSPKNIEYLNVKGTLLIQMGEFDKARAIYTESLALNNQHPNSLFGMGRSWAFEG
                     DLINEKSQDIKDNKLY"
BASE COUNT         4141 a         2275 c         2464 g         4829 t
ORIGIN      
        1 ccattctgat attgtgtgtc catttgcatc cactaccaca tatgtgtaag aaaaagaggc
       61 aagcgagcga acttgtatgc ttgcttccca ttgtccattt tctatgtagt tcatttttaa
      121 tgctttagtt ggaatattgt ttcccaaagc tggtagtgat ccaagtatgc acacttgctg
      181 tccccacgaa gtgtggtact gaattgtaaa ggttagcttc atagtttttt tcaaaaaata
      241 gttaggaata gtttaacgta aaacgaagac aaatgtaata cgtttgtaat ccccaaagct
      301 agtatagagc tacaaaaatg cataaaaaac cttgcaaacg tttgataggt attttagtta
      361 tatgttggta ggtaagattt agtttttgaa taccgcaaca tttgttctgt ataactttca
      421 gtataatgag gtacgatatc tattacttct ccatactcgt tatacacctg ttcgtaagtg
      481 ggatttacaa atcctttata aggtgcaata tccagagcag tataacgttc tagaatttct
      541 ttgtgcaaag agtaatcaat ttggatagcg tatttttcta ctaagtattt ggctgctgtg
      601 taatcgccct cggattttat gcgttgaatt tcggcaagta gttcgccaaa caatgtgcgt
      661 aaagctacat aatcgtgtat gatgatatag gttttattat tttgcactct tttttcgatg
      721 catttttgtt tctgtccttt ttcgaaaacc caattagcaa tgagttgtcg gttgcgcatg
      781 tgcgattctt ctattgtgtc accgagcttt atacgtacca gttgagtcat taatccatta
      841 agcataaatc ggtagtattc agctttgtat gcgtccatat ttggtaacaa accaatttct
      901 accatcatag ggtctgcaat aaaatacaac ccaaataaat cggctcgggt ttcttctatc
      961 gttgctccgt atgcttttaa cgcttctgga gaaaccccat cgagcatttt acctgaccca
     1021 tgtcctacac attcgtgcag gtcggtatgt aatttgtcgg ttatatagcc atatttttcg
     1081 gtcaattcac attcgtgttg gctccacata aattcttcgt taaagccatt accactagca
     1141 gcgtgatgat aagcttccat catattgtcg atggttactg attttgatcc atgtacttca
     1201 cgtatccagt tagagtttgg caagttaatg ccgatagggg ttgcaggata actatcgcca
     1261 ccaagcatgg cattggtaat aacctttgcc gatacgccta caacggatgt tttcttaaat
     1321 ttctcattta ctggagaatg tttttcaaac cattgagcat gattacttat ggtttgggtg
     1381 cgttgggttg cctctttgtt gataaaattg acaatcgcct cccagcttcc ttttcgccca
     1441 agcgggtcgc cataggtttc aataaaacca tttaaaaaat caatttcgga tgaggtgtct
     1501 tttacccaaa gaatggaata ttcatcaaaa gtttgtaagt tgcccgtttg ataaaacgct
     1561 attaatttct cgatgatgag tttttgcttg ctattttcag caaatgccat agcattggat
     1621 aaatggtata taattttttc aatagcagcc gtgtacatgc cattaagttt ccatgttttt
     1681 tctttgatta tgccattttc ttttaccaaa caactgttca aaccataact tattggttgc
     1741 aaatcgctac tatcttgtaa cccattatag aaatcttcag cctcttgttg tgttacattt
     1801 ttgtaaaaat ggttagcaga acttgtaatt aaatcctgat tatcagcgag attcaccctt
     1861 ttcttatcaa tctctgggtt ataaattaag gtaattattt tggtgtaaaa actatctatt
     1921 gtttcatttt tttctagtgg aagaaggctt acaggaactt tttttagtag tgaagtaaaa
     1981 tattctttag aacacgtaat ttcgagttta tcggtagaat aatggtggtg aataccgtta
     2041 gaaaaccata tgcgtttcag gtaaatttca aaagcgttga attcttctac attcttttct
     2101 gtttcattat gtgtttcgta tatggcttcg agtgtgcgtc gaatacacaa attgtgttca
     2161 taatgttggt cgaacaagat atctcgccct tcttgtgctg cttctgaaag atgataaatt
     2221 aattgttttt gttgcaatga caaggcttca aagccagtaa cacgatatct caatatttca
     2281 atatcggcaa atttatctac gtgataatca aatgtatttg ttgtattttg catagttgtt
     2341 ctatttattt cttacatcaa tatctggtgt gtagtgaagt tttttctttt tccattgata
     2401 ggagtcatac gaaaaatcag gaccataaaa atgatacaca tctttgtata tagggtcaga
     2461 tggtgaaaga tggtcgaata caaatcgttt tttgcttgac tcatattcta ataacatgtt
     2521 tacctccttt ttatattcaa aaacggctcg atagagtgtc tttttatctt ttttaaaaag
     2581 gggagtgcca aatgttagtt gccgattact aatttttata gattcaatta tttttacttt
     2641 tgtaatgggt tggtaattgc tgatgcccag taataggtat tctgttttat ctcggctaat
     2701 tttgtgtgct attacgttgt aatacaatgc tccataccac gttgaagggt cgtatttgcg
     2761 tgtttcttca ggtttaaaag ctgtagttgt cgatacaagg tgcaacgagg gtgtgctttt
     2821 tggcttgttt ttgaacataa atagtgccga atagccatat tttccattgc ttaatggata
     2881 attccaagaa aaaacacgca ttagtttgtc aggagcatat atacttccaa tgtattttaa
     2941 ggcaggaaat gaataattaa acgcatttgg ctcttgcaat gttgtgtaaa gcaattgttc
     3001 aatcttttta ttgagttgta tctttgcact atcagattga gtctctttta tatcttcaaa
     3061 ccacgaaaca atcatttgtt cgttttcttg tattgtttgt gcatatacta catggcttac
     3121 acataacaag gtgaaaagaa taagtagtgc gatttttttc attggatttc tattagctta
     3181 cgctactgct acacagtttt aaatattggt aatattatga tttactgggc tatactatgg
     3241 atataatttt ttaattaagt ccattctgtt catctttttt aaatcgttga taatctcatt
     3301 tttatattct ttttgatacc aaaagaaaaa ctttcgttga ttgagttttt gctgaggtgt
     3361 gtttggtgta gaaattttct ccaaggtata tggatgtata cccgtataat acatttccgt
     3421 tgctagtgtc atcggagttg gtgtaaagtc ttgtacctgc tctaatttga aatccaatgc
     3481 tttggttttg gcagctaatt cagccatgtg ctgttgttta caccctggat gactcgagat
     3541 aaagtagggt ataagctgtt gatttaaatg gtgttgcttg ttcacttcgt tgaatagctt
     3601 aacgaatgtt tcaaaatact caaaagatgg ttttcgcatc aagttaagga cttcatcgtt
     3661 ggtatgctca ggagctactt ttagtcgacc agatacgtgg tgtgcaatta attcgttcgc
     3721 gtactccata ttggatgcgt ctgtttgctt attacccgtt ttatgcaata tcatatcata
     3781 gcgaatacca ctaccaacaa atgctttttt tatttcttta atgctgcgta cttttttata
     3841 caattcaatc aatggagcat gattagtatc caaattttta caaatttttg gatgaataca
     3901 cgatggcttt ttgcactttt cgcaaattgt taaatcaata ccttgcattt tatacatatt
     3961 cgctgatgga cctccaatat cgctaatata tcctttaaaa tcaggcattt gagttatctt
     4021 ttcaatttct gctaaaatgg atttttctga acgtgatgca ataaatttgc cctgatgtgc
     4081 agaaatagta caaaaagcac aaccaccaaa acatcctctg tgtaacgtaa cagagtgttt
     4141 tatcatttcg tatgcaggaa tggtttttcc tttgtaccgt ggatgtggca agcgtgtgta
     4201 tggcaaagtg taggtttggt caatttcttt ctccgtcatc gtcggataag gtggattaat
     4261 aatcactgtt tttgttccta catcttgcag taatctacca gctttctgcg aattggaatg
     4321 ttcttcgata accctaaaat tctttgcttg gtttattttt gatgctatac attcctcgtg
     4381 agaaaacaat gagagagatt cccactctcc agttgactga gaattggctg ttttgtcaat
     4441 taaaaaagca gtttgaggga tatcgtgtag ttgttgaaaa gggatgtttt gttgcaaaag
     4501 ttgcaccaat tctacaatcg ctttttcgcc cattccatat acaagtaaat ctgcttgact
     4561 atcaactaag atgcttggca ataatttatc cgaccaataa tcatagtgtg taacacgtct
     4621 taatgaagct tctacgcctc caattacaat aggcgtatcg ggatacaatg atttcagtat
     4681 tgtgcaatag gtaacagtag cgtaatcagg acgataacca gctcttcctt caggcgtgta
     4741 agcatcgttg gagcgtaagc gtttgttggc tgtataatga tttaccatcg aatccatact
     4801 accagccgaa acaccaaaga acaaacgagg ttttcctaat ttcttaaaat cacgtaaatc
     4861 atctcgccag ttgggttgtg gcacaatagc aacttttaag ccttgttgtt caagaattct
     4921 tcccattaca gctattccaa aggagggatg gtcgatatac gcatctcctg agaacagaat
     4981 aacgtccaat tcttcccaac ctaatgcttt tatttctttt tgggtcgtag gtaaaaaact
     5041 ggataatggt atgtcgttat acataattca acacaaattt atataattaa ggctttaaca
     5101 tcgtttggta tgtatcttgg tcgcttacaa tctctatggc acgttttagt tctttgtcat
     5161 tttttaataa ctgttgcaat tcgcctcgtt gaaaataata gcgttttaca atttcagcag
     5221 ccaataattc gctaatttct tctttagcat caataatatc ttgattgata tcatgtttca
     5281 atactttttc taagtctgaa aaatacactt tattttcgtc gtaatatcct tcatttttcg
     5341 ctgtttctat caatttttgt aatccttttt ctgattgcag ttcatatgta aaattctgtt
     5401 ttataatata atctttgtat tgattaaata aatcatctga taccgcaaat tcttcaatcg
     5461 gtggaatcgt tgcatgttgt tgtgtatata tatttacaaa atcgtagaag taagcattag
     5521 atactaaatg ataggtaatc gttttgagtg tcgattcagg aacgagtatg tctggcataa
     5581 tgccttgacc ttctttcact actctaccat tttgtgtagc aaagacatgc tctatgctat
     5641 cagagttagt gcttgctaaa cgtgcaatat gtttactact ctgaatacaa cgtccacttg
     5701 gtgtataata tttggctatt gttattttaa tatttgtgtt ataaggtaaa gggtaggtgg
     5761 attgaattac tcctttacca taggtgtttc gaccaatgat gatgcctcta tctaggtctt
     5821 gcatagctcc tgccacaatt tcagctgcag aagcagagct ttcatttact aatatggcta
     5881 aagggaggtt aggaaataaa ggatcgctac tggttttata cgatctatcc atttccgcgt
     5941 tttttccctt tgttgagact atttcagttc cttttggtag aaataagcca cttatggcaa
     6001 tggcgtcgtt aattactcca cctccattac cacgtaaatc taaaattagt gatgtagctc
     6061 ctttcttagc taagtcttgt aaggcttttt ttacttcttc tgcacaattt tccgtaaaac
     6121 tgttcaaacg aatgtacgct atgttgcttt tacctggtaa tattccagca taagtaacgg
     6181 ggtttaggta tattttctct cttacaatgg aataatgtat gggttttttc attcccatac
     6241 gttcaacaac aatctgtacg gtcgttgatg gagttccttt taacatacta cttacctccg
     6301 aaacagacaa tcctttggta gattttccat ctacttctag tattttatct ccaacttgta
     6361 atccgtatgt atgtgcaggt gcattttcgt ataattcaac gatatgtatt tggctattac
     6421 gtaacgaaat tacagaacct ataccaccat attcgcctgt tgtcatttgc aaaaaatctt
     6481 ttgtatctga ttcgggtatg taattggtat atgggtcgag tttggttaac atagcatcaa
     6541 taccactaga aacaatctct tccataggga tgctatctac ataatatacc tgtaattgct
     6601 tgacaatact attaaagata tctacgtttt ttgctatttc aaaatttttc ttttcagatg
     6661 cttgtgctaa cacgtattga aaagaaacta tagttatgaa tacaaaaaag atttttttca
     6721 tataagtata ttttttatta aacaggtgtt ttttatgcta ctaaacgaat acggtgtgtc
     6781 tattgttaac catacccgaa tatacggtta caaatgtagt atataaaaag caaaaaaccg
     6841 aacatttggt gttcggtttt gctctccctc ttggacttga accaaggacc ctctgattaa
     6901 cagtcagatg ctctaaccga ctgagctaag gaagaatgat gttcaaaaat ttgctctccc
     6961 tcttggactt gaaccaagga ccctctgatt aacagtcaga tgctctaacc gactgagcta
     7021 aggaagagta gtttgcttac tgaacaattt caaaaagcac cgcaaagata tactatttac
     7081 ttgaatttgc aaaaattttt tacttttttt acactaaaaa agagcatttt gtaagttgtt
     7141 tttgagcttt aaaaaacaat aaaaaagcat aaacaactca ttttgaagaa tcaaaacaat
     7201 tatttatgct ttttttgata tagttcttat actaaaattc ccgaactaat tttttttatg
     7261 tcctcattat aatcaatttt aatgggtgta tttttagcat tttctatcgt attattgata
     7321 attaattcgg caatttcatc ttctacgtat ttttgaatag tacgttttaa tggtcttgct
     7381 ccatattgta catcgtatcc ttcgtttgca atatggtttt ttgctgcctc tgtcaattgc
     7441 aatgtgtatc ctaatgattc tatgcgttct actacttgtt ttaattcaat atcaatgatt
     7501 tttaaaattg actcttttga taattgttca aatataataa catcatctac gcggtttaag
     7561 aattcaggtg caaacgattt gtttaatgct tttttaatta cgctatgagc aaattcggta
     7621 tcgtctaagc gagatgttgt agaaaaacca actcctctac caaaatcttt taattgtcga
     7681 gttcctatat tcgatgtcat aatgataatg gtatttttaa aatctacttt tctacccaaa
     7741 ctatccgtta ggctaccttc gtctaatacc tgtaataata agttaaatac ctcaggatga
     7801 gctttttcta tttcatcaag tagtacaatg gagtaggggt tgcgacgcac tttttcagtt
     7861 aattgtccgc cctcttcgta tccaacatat cctggaggtg ctccaattaa acgcgatacg
     7921 ctaaattttt ccatgtattc actcatatca atacgaataa tagcatcttt agagttgaac
     7981 aaaaattcag ctaaaatttt tgctaaataa gtttttccaa cccctgttgg tcctaaaaat
     8041 ataaatgagc caataggttt atttgggtct tttaaaccaa ctctattgcg taaaatagat
     8101 ttggaaattt tctcaatcgc agtatcttgt ccaattactt tttcttttag aatagccgac
     8161 atacgtctta gcttatcaaa ttcaccttcg gcaatttgtt gaacaggaac cccagatatc
     8221 atcgatacaa cctccgaaat gctgttggca tctacagtaa catgttggtt cttaatctct
     8281 tgttcccatt cttgtttggc attttttagc aaaatgttca attccttgat gcggtctttg
     8341 tattcattgg ctaatcgaaa atcttcatct tttacagctt gaagttttga tttattggct
     8401 tcatcaatct cactctccaa aatctcaata ttgcttggaa tggatacatt gtgtaagtgt
     8461 gcccttgatc ctgcttcgtc catggcatca atagctttgt ctggaaaact tcgatcgctt
     8521 atgtacctat cagttaaata gacacatgct tctaatgctt ctggtgtata ggttacatga
     8581 tggtgatctt cgtatctttc tttgatattt tgtaaaatag ccaaggtttc ttcttttgtg
     8641 cttggtttta caattacctt ttggaatcgt ctttctaatg caccgtcttt ttctatgctt
     8701 tggcggtact cgtcaagtgt agtggcacca atgcattgta tcaatcctcg agccaaagct
     8761 ggtttcatca tgtttgcagc atctaacgag cctgctgttg caccagcacc aattaaggta
     8821 tgtatttcgt cgatgaataa aataacatca gggtttgttt ccaattcatt taagattgct
     8881 tttaatcttt cttcaaactg tcctctatac tttgttcctg ctacaatgga tgccatatct
     8941 aacgatacta cacgtttgcc gaataatata cgagatactt ttcgttgtat aatacgtaga
     9001 gccaatcctt caacaatagc cgattttcca acacctgggt cgccaatgag tataggatta
     9061 ttcttttttc ttctacttaa aatttgagat aggcgttcaa tttctttttc tctaccaacg
     9121 attgggtcta actttccttc ttcggctgct ttggtaatgt ctgtaccaaa attgtcgagt
     9181 gcaggtgttt tagaagtgtt ttttgtgtat ttagttgatg tagagaaggt gcttgtttta
     9241 ccattttttt ctacagaatt tgagctaaat tcactatctt cgtcttcgcc atcttcgtca
     9301 ttcgagttta ctccgttttt tttatcaata gatccaatgt cttttttttc tactaaagct
     9361 cttactgtat catacgtaat gttttctaca ctaaaaattt ctgtaaccag atttgtttta
     9421 tcttttaata ttgctagtaa aaggtgctca gaatcagcca tatagttgcc aaattgacga
     9481 gcctcaagta caatgaattt gagtattttt tcagttgatt ttagcagggg tatattggat
     9541 ccaatcatca cgttttctga tgttcggata ctcgattcaa ttgttttttt tatacgcaat
     9601 aaatctaagt taagtgacaa aagtacgtca aaagctttac cctctcctgc acgaataatt
     9661 gccaaaagta agtgttcggg tccaatgtaa ttattgccca aacgttctgc ttcttcgtgg
     9721 ctatatcgta aaatatcggc aacattgtcc gaatagttca tttcattttc catattctta
     9781 cgtgtttgat aaacattatt gttagtgttt gctaactatt ttgcacatat tgtattgcaa
     9841 aggtatagtt tttctttaaa aattgtgtat tgtttacaca ttcttaacaa cagattacaa
     9901 atactatgcc aacaatatat gtgtgtgtta aaaagatttt tgacgaaagg tttttgtatt
     9961 tgaataaaaa gtagtacctt agtgcgaatt ttcagaaaag gtatttgttt tacttaacta
    10021 ttttataaat gattgatcaa gaaaggatta taaaaatcaa cattgaggag gagatgaagt
    10081 cagcatacat tgattactca atgtctgtta ttgtatcgcg tgcacttccc gatgtaagag
    10141 atggattgaa gcctgtacat cgtagagtat tgttcggaat gaatgagttg gggagtaact
    10201 ccaataaacc gtataaaaaa tctgctagaa tagtagggga agtgatgggt aagtatcacc
    10261 ctcatggcga ttcttctatt tatggtacat tagtgcgaat ggcacaagaa tggtcaatgc
    10321 gttatcaact ggttgatggt cagggaaact ttggctctat tgatgccgac gaacctgctg
    10381 ctatgcgtta tacggaagcc cgtatgcatc gcttggcaga agaaatgttg atggatattg
    10441 ataaagatac cgttgatttt aaacttaatt ttgacgatac attaaaagaa ccaactgttc
    10501 tgccaactcg tatacccaat ttattgataa atggagcatc gggaattgca gtaggtatgg
    10561 caaccaatat ggctcctcat aatttatctg acacgataga tgccattgtg gcatatattg
    10621 acaacaaaga ggtagatgat gaagagttaa tcaaattaat taaagctcca gattttccaa
    10681 caggaggtat tatttatggt tatgcgggta ttagagatgc ttatttaaca ggtaaaggtc
    10741 gtgttgttat aagaggtagg gtaactattg aggtagaaaa agatcgcgaa cgcattgtta
    10801 ttaccgaaat accttattta gtaaacaaag cagaactaac taaacatatc tctgacttag
    10861 taaatgaaaa acgtattgaa ggaatttcta atatttcaga cgaaagtaac tacgagggta
    10921 tccgtttggt tgttgaaata aaacgtgacg ctaacgccaa tgtggtgcta aacaatttgt
    10981 acaaacaaac ttctttacaa acatctttta gtgtcaataa tattgcactg gttaaaggtc
    11041 gtcctcgctt attggtatta cgtgatttaa ttgaattgtt tgttgaacat agacacgaag
    11101 ttgttgtacg tcgcacgcaa tttgaattga aaaaagctca ggaacgtgct catatagtag
    11161 aaggactcgt tattgcatct gataatatcg acgaagttat tgccattatt aaagcagcga
    11221 gcacgcctaa cgatgcaatg gaaaaactga tggtgcgttt tggcttgtct gaactacaag
    11281 tacgtgctat cgtagatatg agattatcgc aactcacaaa tatacagcaa gataaattaa
    11341 gacaagaata ccaagattta cttgttttaa ttgccaaatt agaagatatt ttggggaatg
    11401 aaacattgcg tatgcaaatt atcaaagacg aattaatcga agtaaaagat aaatatggag
    11461 atgagcgtcg ttctgaaata gtatatgctt ctgaagattt taatccagaa gatttttatg
    11521 ctgatgaaga aatgattatt actatatctc atttgggata tattaaacgt actccgttgt
    11581 cagaatataa attgcaaaat agaggaggtg ttggttctaa agggggagaa tcgagagatg
    11641 aagactttat agaatacatt taccctgctt ctatgcacaa ttatatgtta ttctttacac
    11701 aaaaaggtcg ttgttattgg cttaaagtat acgatatccc tgaaggttca aaaatatcta
    11761 aaggtagagc tattcaaaac ctcttaaaca ttgaatcaga tgataaagta aatgcattta
    11821 tacgcataaa acaattacaa gatcctgaat acaatcaatc gcattattta ttgttctgta
    11881 ccaaacaagg tgttattaaa aaaacacgtt tagatgcgta ttctcgccca cgtcaaaatg
    11941 gggtaaatgc cattactatt agagaagatg atgatgtaat ccaagtacgt ttgacagatg
    12001 gagatagtga agtagtgatt gctaataaaa atggtagagc tatccgtttc cacgaatcaa
    12061 aggttaggga aatgggacgt actgctactg gtgttcgtgg tattacactt gacgaagatg
    12121 gacaagatga ggtaattggt atgattacca ttccgaaaga agaaaaaaca aaggaaagta
    12181 ttttagtggt ttctgaaaaa ggatatggta aacgtacatt gattgatgaa attgatgaaa
    12241 atggaaatgc tatagtagat gaaaatggag atcctgtgcc tgtttacaga ataactaata
    12301 gaggaggtaa aggtgtaaaa accatgaata ttactgataa aacaggtaaa ttagtagcta
    12361 taaagaacgt atctgataac aatgatttgg tgattattaa caaatcgggt attgctatac
    12421 gcctagaagt agcaagtatg cgtattatgg gacgtgctac acaaggcgta aggctaatca
    12481 atctcgaaaa acgtaacgat gaaatagctt ctgtttgtaa agttgtttca gaagagaaag
    12541 aaaacttaga tgaagtttcg ccagaattac tagatgcaga aaataacttt aattcgattg
    12601 aaggtattgc taccaacgat gaaacaaata atgaataata aacaaaaaaa tctatttatg
    12661 aaaaaaacac tatggatgac actaggtttg ttattgttta caatagcaac aggctgtgca
    12721 caaaaatcgc aagtaaataa ggcatataat cgtttgttta ctgaaccaat cgattatgct
    12781 gcagcaaccg aagatattga agttgcaaaa aaagattcta caactgctac acaatcccgt
    12841 acttggtatg ttgctggtcg tattggctat acaatggcaa attcagaagt aacgaagatg
    12901 cgtatgcaac aacctgctaa cgatgaaaat ttgtatcaag gattaaaaca aatgtacgaa
    12961 aattatgttg ttgcagacaa gttcgatggt gtagtagata aaaagggtcg tataaaatat
    13021 tctcaacgga gaaatataaa agccgatttt aaagaaatgc atccttttta tatcaatgct
    13081 ggtgctacta tgtttgaata taaagagttt gccaaagcgt atacattgtt taatcagtac
    13141 attaagattg ctgatttagc tatatgggaa gaaaaagatg cgataaaaat agattccact
    13201 tataatacaa ttcagtttta tgcaggtatt gttgcatcta atatggatag tactcaactg
    13261 gctattaagc actttaaaaa tttaataggt acttcgtatc cacaaaagga ggctgtttac
    13321 gagatgcttt attcattgta tcaacatgcc aatgatacag taaattttat atcaatatta
    13381 aaagaaggcg ttgaattgta tcctgaaagt ccttttttta taggtaattt aattaattat
    13441 tatgctacag caagtaaatt tacagaagca ttagactatt taaatgaagc cttggaacgt
    13501 tctcctaaaa atattgaata ccttaatgtg aaaggtactt tgttgataca aatgggtgag
    13561 tttgataaag caagagctat ttatactgaa tcactagcac taaataatca gcatccaaat
    13621 tcgttgtttg gaatgggtag atcatgggct tttgaaggag atttaattaa tgagaaatcg
    13681 caagatatta aggataataa gttgtataa
//
LOCUS       DAOSRP010000050        13515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4646_length_13515_cov_5.878455, whole genome shotgun sequence.
ACCESSION   DAOSRP010000050 DAOSRP010000000
VERSION     DAOSRP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_13515_cov_5.878455"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLJ82_04460"
     CDS             complement(<1..446)
                     /locus_tag="PLJ82_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOR40790.1"
                     /translation="MMDGKSLPITEDKQNALRQLFPEVFSEGKIDWEKLKATLGEDIT
                     FSNERYVLNWAGKSDAFKVLQAPTTKTLIPAKDESINFDETENIFIEGENLEVLKVLQ
                     KSYFGKVKMIYIDPPYNTGNDSFIYPDKFAETKEEYQKRVGDKDEEG"
     gene            complement(692..2098)
                     /gene="asnS"
                     /locus_tag="PLJ82_04465"
     CDS             complement(692..2098)
                     /gene="asnS"
                     /locus_tag="PLJ82_04465"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOR40791.1"
                     /translation="MEAIKRKKIVEALKSSSFGEKVNIKGWVRTKRGNKTVNFIAIND
                     GSTINNIQVVVDMEKFGEEILKPITTGACVSINGILTESLGKGQSVEIQATEIELYGA
                     ADPDTYPLQKKGHTLEFLREIAHLRPRTNTFGAIFRVRHQMAMAIHNFFHNKGFVYVH
                     TPIITGSDCEGAGQMFQVTTLNLYNLKKNEQGQIDYTDDFFGKQASLTVSGQLEGELA
                     AMALGEIYTFGPTFRAENSNTPRHLSEFWMIEPEMAFYEIADNMQLAEDFVKHCIQWA
                     LDNCQDDIQFLSEMYDKELVERLKFVVDNEFIRLTYTEGIEILENAVKNGKKFEFPVG
                     WGTDLQSEHERYLVETHFKKPVILTDYPKEIKSFYMKQNADGKTVRAMDVLFPKIGEI
                     IGGSQREENYEKLRQRAAEMGVPEKDIWWYLDTRRFGTAPHSGFGLGFERLLLFVTGM
                     ANIRDVIPFPRTPNNAEF"
     gene            complement(2219..3751)
                     /locus_tag="PLJ82_04470"
     CDS             complement(2219..3751)
                     /locus_tag="PLJ82_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOR40792.1"
                     /translation="MFSPEDELQLANKGIAKEKLETQLLNFEKGFPYAHLQQAATPGN
                     GIVVLNNEQISDYIHYYEKQLATIKALKFVPASGAASRMFKRLSEFLEAHVHNAHYLH
                     TYYPDVAAFMHHISKFAFYEELKTCIEKDGETIEDLLQKEQYNKIILYLLTPLGLNYG
                     NLPKALLSFHRYAENTRTAFEEHLVEGVAYAKNVQNEVAVHFTISPEHKSAFIEKMNH
                     VLPIYEDAFSVTFKLSFSEQKPSTDTIAVNENNEIIRNEDGSMLFRPGGHGALIENLN
                     DCDADIIFIKNIDNVVVDTLKQSTYTYKKALAGLLLSIQAATFDLLKKLDGNVDDATL
                     KIIEDFAKNTLYIHVPSQYAVAPKEEKIAFWKKTLNRPIRVCGMVKNEGEPGGGPFWV
                     LNEQNEESLQVVESSQIDYKNKLQERIAVKASHFNPVDIVCATKNMHGEKFYLPDFVD
                     PKTGFISSKSKDGKTLKAQELPGLWNGAMANWISIFVQVPISTFNPVKTVNDLLRKEH
                     QA"
     gene            complement(3781..5052)
                     /locus_tag="PLJ82_04475"
     CDS             complement(3781..5052)
                     /locus_tag="PLJ82_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOR40793.1"
                     /translation="MKNICFYFQLHLPYALKRYRFFEIGNDHYYYDDFQTEETIRLLV
                     EKSYLPANKTILDIIHHSNKAFKCAFSISGCTLEQLEQYAPEVIDSFRELAATGCVEF
                     LVEPYAHSLASVFNELEFERQVQLHAKKIEDLFGYRPTTLRNAELIYSDEIGEKAYQM
                     GYQSIVVEGGRDILGWKSAHYLYHHPHKKNLKILMRDYKLSDDITFRFSDKSWTDYPL
                     TADKYIQWIANAPTEEELINIWLGYETFGLKQDASSGILDFLKAIPYQAIVHGIEFIT
                     PTEATQKLESKDSLSAPYTSAWGSEEKDLSSWTGNDLQQEALQKLYAVTERVHLCKHK
                     TLKRDWLMIQSSDYFRYMSHRNAYNSPYDSAYDAFVNYMNILSDFLLRVDAEYPSSIE
                     NEELNALLKTITEQEKEISNLTKQLYTKNKK"
     gene            complement(5065..6321)
                     /locus_tag="PLJ82_04480"
     CDS             complement(5065..6321)
                     /locus_tag="PLJ82_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40794.1"
                     /translation="MKALMFGWEFPPQILGGLGTASYGLTKGMAQQTDMEITFVLPKP
                     NGDEDQSFLRLVGANHVPVVYKETSNEYIEQIIGKLTRLDDYLHYRNNIRYDFSRIST
                     NELGCIYFSGRYPDNLVEEISNYEAVASVLAHSLDFDIIHSHDWLTYPAGKFAKQISG
                     KPLVVHVHATDFDRSRGNVNPTVYAIEKAGMDAADHIITVSNLTRQTVIDKYHQPPHK
                     VTTVHNAVEPLEHAETVIKAQRSEKLVTFLGRITMQKGPEYFVEAAHRVLEKTNNVRF
                     VMAGSGDMMHAMIDLVAKRNIADRFHFTGFLKGKQVHQLLADSDVYIMPSVSEPFGIS
                     PLEAMQVGTPSIISKQSGCAEILAHAIKTDYWDIDAMADAIYAIVHYPAMHKSLRNEG
                     KAEVDSITWEKAGLKVRKIYDMVLHQ"
     gene            complement(6333..8276)
                     /locus_tag="PLJ82_04485"
     CDS             complement(6333..8276)
                     /locus_tag="PLJ82_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOR40795.1"
                     /translation="MNFLNFDKNLLINLEKSLTKEMIRTNRAGAYCSSTLVDCNTRKY
                     HGQLVVPLPHIDNHNHVLLSSIDETICKHGAEFNLSVHQYPNNHIFPNGHKYIREFDC
                     NSISKTVFRVGSVIIAKERCMVFYENKVLIKYTMLETQSATTLRLKPLLAFRSVHELC
                     KENSLINNSYTEIANGISVQLYPTYPTLHIQLSKSNTYEHTPHWNKNIYYAKEAERGF
                     ECTEDLYAPGTFEVSLKQGESVIVAVGTESSAIKTLKTLWEKEVSKRNPRTDLYNCLR
                     NSAVQFYTRTQDNWYLLAGYPWFVVRARDQFISLPGCTICIGETEMFEKIMHSSIRAI
                     KQFMEKGTLSDNIQELDLPDSLLWCIWCLQQYGVYVSEEEMIAKYGDFIVDVVEFIRR
                     QKHPNLFVHSKGLLHISGKEAPATWMNAEENNQPITPRTGFVVEINALWYNALKCSET
                     IHEINGNSKQSHILHYQAELAGNEFIQTFWNGMYLVDYVDGIERNMEVRPNMIFATSL
                     PYSPLTKQQQKSILDICTKELLTPKGLRSLSPKSGSYRPNYVGGMLERNRNYHNGPVW
                     TWTLGAYTETYLKIHKKSGVFFIERIVVGLEAEMKKLCIGTLSELYDGNPPYKGHGGM
                     SFAMNVAEILRVLRLLQNIQQDE"
     gene            complement(8406..10166)
                     /locus_tag="PLJ82_04490"
     CDS             complement(8406..10166)
                     /locus_tag="PLJ82_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40796.1"
                     /translation="MKRIIYILPLVLFLANTSLSFSQSNVSRDVTLEREYNPTAIDAK
                     KINTVPKTEEYTIDKKDITYTISSSPATVDKSFNPLATDTYKDTQLGEIKNGVFRAGI
                     GSHWQLLGDFYYPLLQGDTYLLDLNVSHQSAWGKLKIEDNEPTRAMFATTRAGFTFEN
                     QFRNSRLVSGIHFLHKGFDYYGKSNIDTSLWVGDTQREIVKIKADSLLESGIGSFTKA
                     GTNFRLFSTNQQSSFQHNSNLSYQYFASHIAVEEHNFYVGTNLSGSLETGRIGVSVDV
                     DNYFYKKPLELSANYAIKETDFGNTSVIKLTPYYIFVGKNWDISMGASLFAIIGATQR
                     PVSGAANIVGKAALVPDLFYLYGGIVGDIKHNSYSSILTENNYVSPDVSLQDTYTPLN
                     IYTGLKLKVMDGLLFDGYLGYKIIRDQYFFINRTVNYVAPMPIGTPASAYANTFDVVT
                     EKDASLLSVDLSLTFDKIDGLDVSVNSRYNKWNVSQNAFAWHKPTWEIGLQASYQIDA
                     KWKAGIAYNYMGGRNALVDTEVVAMNNIHDVNATVSYKVFDWLHVFGSMRNAINANYD
                     TYYGYKAFGINGMVGASVTF"
     gene            complement(10220..13273)
                     /locus_tag="PLJ82_04495"
     CDS             complement(10220..13273)
                     /locus_tag="PLJ82_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40797.1"
                     /translation="MKYLHISIFCLLFSLTVTPLFSQKSPFNTTAAELFADGKSFFYN
                     NNYAASARYFEEFLQFDQRDNDEMVREAEYYIARSAFELRKNDAQIKLEAYQTKYPYA
                     TDVELTNLYIGILEYESGRFKQAIRRFESIKTDRLRESDLVKLQFYKGYAYHQQENFE
                     KSRFEFQQVLRRGTNTPYYDAATYYFAYAEQRLGNFDSALTHFLRVENHPEFHTSAPF
                     FIAQLYYQQGNCDKVLNYGKEIIKKYPKNNRRNEVLRMMGECSFLAKDYSSSIHYLTQ
                     YEQQVKRISREDFYMLGIAHYNVGEYEKAIANLSKTTNREDLLAQNSYLYLGMGYVKL
                     NDKKNARLAFETASRFTFDKGIQEEALYNFAVVSYEQSFSPFNESVVAFERFLTEFPS
                     SKRKDKVYEYLANSYLTTKNYGEAYRSIQNLKTDNKKIKEAEERILFNMGIVAVANNN
                     YQQATSHFEKILKGKSYNADFKTRSFYWYGESLLNTGYYTQAEKNFQTFLQSPKAQKL
                     AEYDIAHYSMGYTYYLLKNYTDAVTWFKRYIALKPDNNLMVLDANNRIGDSYYQNRQF
                     AEAKRYYNQAIATGAQLPGADYATFQKGFVAGLERNHNEKISTLRNLITTYPQSEWVD
                     DAYIEIGKTYVVLKENDKAIETFAEIEKKFSRNHQLIRQARLQIAMLQYNDGKTDAAL
                     ESYKQLVRTYPSSEEARISLEAIENILVEDNRVSEYTEFVNALGKGSIQIAPSREDSL
                     LFRAAQRVYSRNEIPQAISSFEKYLDTYPKGRYSTTARYLLASCYYTQKQPAKALPLY
                     KALLSDKENPNQEETLARAAEISYDLGQYAESMEYFKQLEAIGSSENKMVARIGLMRA
                     SYILKRYDETITYATNFISLHSNNLELLREAQHKRMLSYLELNKAQNALPDMQILAKD
                     TRNAFGAEAKYLLATHYYLAKQYESAEKEVFDFIDKGTPHHKWLAKAFILLADVYMEQ
                     DNYFEAKQYLLSLKSNYPKDDVEIASEINTRLEEIAKIENEKVNN"
BASE COUNT         3826 a         2523 c         2406 g         4760 t
ORIGIN      
        1 ccttcttcgt ctttgtcgcc tacacgtttt tggtattctt ctttcgtttc agcaaactta
       61 tcggggtaaa taaatgaatc atttcctgta ttgtaaggag ggtcaatgta aatcattttt
      121 acctttccaa aatagctttt ttgcagtact ttcagcacct caaggttttc gccctctata
      181 aaaatgtttt cggtttcgtc aaagtttata gattcgtctt ttgcagggat taaagtcttt
      241 gtagtaggtg cttgcaacac tttaaaagca tcgcttttac cagcccagtt taacacataa
      301 cgctcgttgc taaatgtaat atcctcgcca agtgtggctt tcagcttttc ccaatcaatt
      361 ttcccttcgc taaatacttc tggaaaaagt tgtcgcaaag cgttttgttt atcctctgta
      421 ataggaagac ttttcccgtc catcatgtta tttgtgtgtt ttttttgaaa tttattgcta
      481 ttttcaacgt attcctatgt tgttttactt tgtatttgat gcaaaatata gccgtgcacc
      541 aatatgtgca aatgtacaaa aaatttctgc tgtaaaaaat atttagttag gtaaaaatat
      601 tttctttcaa ttattgggct aatccataaa aaaagcccct aaaatatttt tcaaattcag
      661 gagctttatt ttattcaaaa gagttttttc atcaaaactc agcattatta ggagtgcgtg
      721 gaaatggtat tacatcgcgt atatttgcca taccagtaac gaaaaggagt aatcgttcaa
      781 atcctaatcc aaagccagag tggggggctg tgccaaagcg gcgagtatcc aaataccacc
      841 aaatatcttt ttcgggcaca cccatttctg ctgcacgttg gcgtagcttt tcgtagttct
      901 cttcacgctg agaacctccg atgatttcgc caatttttgg gaataacaca tccatagcac
      961 gtaccgtttt gccatcggca ttttgtttca tataaaacga cttaatttct ttagggtagt
     1021 cggtcaatat aacgggtttt ttaaagtgcg tttctaccaa gtagcgttcg tgttctgatt
     1081 gcaagtcggt gccccagccc acaggaaact cgaatttttt accgttttta acggcatttt
     1141 ctaatatttc tatgccttcg gtgtaggtca atcggataaa ctcattatct accacaaatt
     1201 tcaaacgttc taccagttct ttatcgtaca tttctgataa aaattggata tcgtcttggc
     1261 aattatccaa agcccattgt atgcaatgtt ttacaaaatc ttcggctaat tgcatattgt
     1321 cagcaatttc gtaaaacgcc atttcaggct ctatcatcca aaattctgat aagtgacgag
     1381 gcgtattgga attttcagcc ctaaaagtag gtccaaacgt gtaaatttcg cccaatgcca
     1441 tggcagcaag ttctccttcg agctgacccg atacggttaa actggcttgt ttgccaaaaa
     1501 aatcgtcagt atagtcaatt tgtccttgtt cgttcttttt aaggttgtat aagttgaggg
     1561 tagtaacctg aaacatttgt cctgcaccct cgcagtcaga cccagtaatg atgggagtat
     1621 gtacgtatac aaatcctttg ttgtgaaaaa agttgtggat agccattgcc atttggtggc
     1681 gcactctaaa aatggctcca aacgtattgg tacgtggacg taaatgagct atttcgcgca
     1741 aaaactccaa ggtatgtcct ttcttttgca aaggatatgt gtcggggtca gccgctccgt
     1801 aaagttctat ttctgtagct tgtatttcaa cggattgtcc cttgcccaac gattcggtta
     1861 atataccatt aatacttaca caagctcctg ttgtaatggg ttttagaatt tcttctccaa
     1921 acttttccat atccactacc acttgtatat tattgatagt gctaccgtca ttaatggcga
     1981 taaaatttac agttttattt cctcttttcg ttcttaccca gcctttaata ttcactttct
     2041 caccaaatga tgaggatttt agggcttcta caattttttt tcttttgatt gcttccatct
     2101 gctgtaaatg ataaaatttc gtaattaagc cacaaaggta agtattttgc aattaattgt
     2161 ggtaaaattg ccgttaaatt ctacataaaa aagtcccttt gcgggacttt gtctattttt
     2221 aggcttggtg ttcttttcta agcaggtcat ttaccgtttt tactggatta aatgtgctga
     2281 taggtacttg tacaaatatg cttatccagt ttgccattgc tccgttccat aatcctggta
     2341 attcttgtgc ttttaaggtt tttccgtctt tcgatttgga ggaaataaat ccagttttgg
     2401 ggtcaacaaa atcgggtaaa taaaattttt ctccatgcat attttttgtg gcacagacaa
     2461 tatccacagg gttaaaatgg cttgctttta ctgcaatgcg ttcttgtagc ttgtttttgt
     2521 agtcaatttg cgatgattct actacctgta aactttcttc attctgttcg tttagcaccc
     2581 aaaatggacc accaccaggt tctccctcat tttttaccat accacacaca cgtatcggtc
     2641 tgtttagggt ttttttccaa aaagcaattt tttcctcttt cggtgctact gcatattgac
     2701 taggtacgtg aatatatagg gtgtttttgg caaaatcttc tattatctta agtgtggcat
     2761 catctacgtt gccatcgagt ttttttaata gatcgaaagt tgctgcttgt atgctcagaa
     2821 gtaagcctgc taaggctttt ttataggtgt aggtcgattg ttttaaggtg tcaactacta
     2881 cattgtcaat attttttatg aaaataatat ctgcatcaca atcgtttaag ttttcaatca
     2941 acgctccgtg tcctcctggt ctaaaaagca tactaccatc ttcattccga ataatttcgt
     3001 tgttctcgtt tactgctatg gtatccgtcg atggtttttg ctcagaaaaa gatagtttga
     3061 atgtaacact aaatgcatcc tcgtatatgg gtaatacgtg gttcattttt tcgataaaag
     3121 ctgatttgtg ttcgggcgaa atggtaaaat gcacagcaac ttcattctgt acattttttg
     3181 catacgccac tccttctact aaatgttctt cgaatgctgt gcgggtgttt tcggcatacc
     3241 gatgaaacga tagtagggct ttgggcaaat tgccatagtt aagtccgaga ggggtaagca
     3301 aatacaaaat aattttgttg tattgttctt tttgtaagag atcttcgatg gtttctccat
     3361 ctttttcaat acaggttttt agctcttcgt aaaaggcaaa tttagatata tgatgcataa
     3421 aggcggctac atcagggtaa tacgtatgta aataatgggc attgtgcaca tgtgcctcta
     3481 aaaattcaga taatcttttg aacatacgag atgcagcacc agaagcgggt acaaatttaa
     3541 gagctttgat tgtagctagt tgtttttcgt aataatgaat atagtctgaa atttgttcgt
     3601 tgtttaacac aacaattcca tttccaggcg tagccgcttg ttgtaagtgg gcatatggaa
     3661 aacctttttc aaaattgagc aattgagttt ccagcttttc ttttgctatg cctttgtttg
     3721 ctaattgtaa ttcatcttct ggagaaaaca tagttcatca tttttttgcg aagttaggca
     3781 ttatttttta ttttttgtgt ataattgttt ggtcaaatta ctaatttctt tttcttgctc
     3841 tgttattgtt ttgagtagtg catttaattc ttcgttttca atagagctag ggtactcggc
     3901 atctacgcgc aaaagaaaat cagacaggat gttcatatag ttaacaaaag catcataagc
     3961 cgaatcatag ggtgaattat aggcatttct gtggctcata tacctaaaat aatcggatga
     4021 ttgaatcatg agccaatctc ttttcaatgt tttgtgtttg cacaaatgaa cacgttcggt
     4081 aacggcatat aatttctgaa gggcttcttg ttgcaaatcg tttcccgtcc aactacttag
     4141 gtccttttct tcacttcccc atgcactggt ataaggagcc gataatgaat ctttggactc
     4201 aagtttttgt gttgcttctg ttggggtaat aaactcaata ccgtgaacta ttgcttgata
     4261 tggtatagct tttaaaaaat ccaaaatgcc agaagaagca tcctgtttta gaccaaaggt
     4321 ttcgtatcct agccagatat taattaactc ttcttcagta ggagcgtttg ctatccattg
     4381 tatgtattta tctgccgtta gagggtagtc tgtccaactt ttgtcggaaa atcgaaaagt
     4441 gatatcatcg cttagcttat aatctcgcat caatattttt aggttctttt tgtgtggatg
     4501 gtggtacaaa tagtgagcac ttttccaacc tagtatgtct cttcctcctt ctaccactat
     4561 cgattggtat cccatttgat atgctttttc tccgatttca tccgaataaa taagctctgc
     4621 attacgcagt gttgttgggc gatagccaaa taaatcttca atctttttgg cgtggagttg
     4681 cacttgtcgt tcaaactcca attcattaaa tacagatgct aacgaatgag cgtaaggctc
     4741 tactaaaaac tcaacacatc ctgttgcggc tagctcacga aagctgtcaa ttacttcagg
     4801 ggcgtactgt tctagttgct cgagggtgca tcctgaaata gagaaagcac atttaaaagc
     4861 tttgttagaa tgatggatga tatcaagaat agttttgtta gcgggaagat acgatttttc
     4921 tacgagcaaa cgaatagttt cttctgtttg aaaatcatca taataataat ggtcgttacc
     4981 tatttcaaaa aaacgatagc gtttaagtgc atagggtaga tgtagttgaa aatagaaaca
     5041 gatatttttc atattcagtc ttttttattg atgtaaaacc atatcgtaaa tttttcgaac
     5101 tttcagtcca gctttttccc acgttatgga gtctacttct gcttttccct cgtttcgtag
     5161 gctcttatgc atagcaggat agtgtacgat ggcataaata gcatctgcca tggcatctat
     5221 atcccaatag tctgttttaa tagcatgtgc taaaatttcg gcacagcccg attgtttcga
     5281 aattatactt ggtgttccaa cttgcatggc ttctaatggc gaaatgccaa aaggctccga
     5341 cacagatggc ataatatata catcgctgtc agctaataat tgatgaactt gtttcccttt
     5401 caaaaatcct gtaaaatgga accggtcggc aatattgcgt ttggctacta aatcaatcat
     5461 tgcatgcatc atgtcgccac tgcctgccat tacgaaacgc acattattgg ttttttccaa
     5521 taccctatgt gccgcttcta caaaatattc tggtcctttt tgcatggtga ttcgccccaa
     5581 aaatgtaact aatttctctg aacgttgtgc ctttatgaca gtttcggcat gttcgagcgg
     5641 ttctacggca ttatgcaccg ttgttacttt gtgtggtggt tgatggtatt tatcaattac
     5701 tgtttgtcgg gttaagttgc ttacggtgat aatatgatcc gcagcatcca ttccagcttt
     5761 ttctatggca tataccgttg ggtttacatt gccacgactt ctatcaaaat cagtagcatg
     5821 tacatgtact actaagggct ttccgctaat ctgtttggca aatttacctg ctggatacgt
     5881 aagccaatcg tgcgaatgaa ttatgtcaaa gtcgaggcta tgggcaagta cgctagctac
     5941 tgcttcgtag ttgctaattt cttctaccaa attatctgga taacgcccag aaaagtagat
     6001 acagcccaat tcgttggttg aaatacgcga aaaatcatac cgaatattgt ttctataatg
     6061 caaataatca tctaaacggg ttagttttcc aattatctgc tcaatatact cattcgatgt
     6121 ttctttgtac actactggta catgattggc tccaacaagg cgtaaaaaac tttggtcttc
     6181 gtcgccattg ggctttggta atacgaacgt tatttccata tctgtttgtt gagccatgcc
     6241 ttttgttaag ccatagctcg ctgtacctag ccctcccaaa atttgtggag gaaattccca
     6301 gccaaacatc aatgctttca tattcgtata gtttattcgt cttgttgaat gttttgcaat
     6361 aaccgcaata cccttaaaat ttctgctaca ttcatagcaa acgacatccc tccatgtcct
     6421 ttatatggtg gattaccatc gtatagttcc gataaggtgc caatacataa tttcttcatt
     6481 tctgcctcta gtccaacaac gattcgctca ataaaaaaca ctccgctttt tttatgtatt
     6541 tttagatatg tttctgtgta tgcccctaat gtccatgtcc aaacgggtcc gttgtggtaa
     6601 ttacgattac gttcgagcat tcctcctaca taatttggtc gataagagcc actttttggg
     6661 cttaacgacc gtagcccctt aggtgtgagc aattctttgg tgcaaatgtc taaaatcgac
     6721 ttctgttgct gtttggtaag aggagaatag ggtagcgagg tggcaaaaat catattcggt
     6781 ctcacttcca tatttctttc tattccatca acataatcaa ccaaatacat gccgttccaa
     6841 aacgtttgta taaattcatt tcctgccaat tctgcttggt aatgtagaat atgcgattgc
     6901 ttgctgtttc cgttaatttc atgtattgtt tctgaacatt ttaaagcatt gtaccataaa
     6961 gcattaattt ctacgacaaa gcctgttcgg ggagtaatag gctgattgtt ttcttctgca
     7021 ttcatccatg tggcaggagc ttcttttccg ctaatatgta ataatccttt tgaatgcaca
     7081 aatagattgg gatgtttttg tcttcgaata aactctacta catcaacgat gaaatcgccg
     7141 tattttgcta tcatctcctc ttcggataca tatactccat attgttgtaa acaccaaata
     7201 caccataaaa gactatctgg taaatccaat tcttgaatgt tgtccgatag tgtccccttt
     7261 tccatgaatt gtttgatagc ccgtatggat gaatgcatga ttttttcaaa catttctgtt
     7321 tctcctatac agatagtgca accaggaagg gatataaatt ggtcgcgagc tcgtactaca
     7381 aaccatgggt agccagctaa taaataccag ttatcctgtg tgcgtgtata aaattgcacc
     7441 gcagagtttc gtaaacaatt atataaatct gtacgaggat tgcgtttact tacttctttt
     7501 tcccacaatg ttttcagtgt ttttatagca cttgattctg tacccacagc tacaattaca
     7561 gattctcctt gctttaggct tacctcaaat gtgccaggag catataagtc ttctgtacat
     7621 tcaaaacctc gttctgcttc ttttgcataa tagatatttt tgttccaatg cggagtatgt
     7681 tcgtaggtgt tgctttttga aagttgtata tgtaatgttg ggtaagtagg gtatagttgc
     7741 acactaatac cattggcaat ttctgtgtaa ctgttgttta tcaacgaatt ttctttacac
     7801 agttcatgta ctgaacgaaa agctaagaga ggttttaatc gtaaagtggt tgccgattga
     7861 gtttcaagca ttgtatattt tatcaatact ttattttcgt aaaataccat acaacgctct
     7921 ttggcaatta ttacactgcc tacccgaaat actgtttttg agatggaatt gcagtcaaac
     7981 tcacgaatgt atttatgtcc attcgggaaa atatggttgt ttggatattg atgtacgctt
     8041 aaattaaatt ctgcaccatg tttgcaaatt gtttcgtcaa tagaggataa aagtacgtgg
     8101 ttatgattgt ctatatgtgg aagcggcact accagttgtc cgtggtattt gcgtgtattg
     8161 caatctacca aggtagagct acaataagct ccagcacgat tagtgcgaat catctctttg
     8221 gtgagcgatt tttctaaatt aattagcaaa tttttatcaa aattcaaaaa attcatactg
     8281 cttgtgtaag aaataggtaa aaattgcaat tctaactatg ataaatgcaa aaatacataa
     8341 tattgtgata taaaaaaggg aaagaagtgt tttcttcttt cccttaaaaa aaaggactat
     8401 gtactttaga atgtaaccga agcacctacc attccattta taccaaaggc tttatacccg
     8461 taataagtat cgtaatttgc attgattgca tttcgcatag atccaaatac gtgtagccaa
     8521 tcgaatactt tataagaaac agtagcattt acatcatgta tattgttcat ggctaccact
     8581 tctgtatcaa ctaaggcatt tctacctccc atatagttat aggcaatacc tgctttccat
     8641 ttagcatcaa tttgatagct ggcttgtagt ccaatttccc atgttggttt atgccacgca
     8701 aaagcatttt gacttacatt ccatttgtta taacgactgt taaccgatac atctagtcca
     8761 tctattttgt caaaagtaag cgataaatca accgataaaa gcgatgcatc tttttctgtt
     8821 accacatcaa atgtgtttgc gtatgcactt gcaggtgtac ctataggcat aggagctacg
     8881 taattaaccg ttctgttgat gaaaaaatac tggtcgcgta taattttata tcccaaatac
     8941 ccatcaaata aaagcccatc catcactttt aattttaatc ctgtgtaaat atttaggggt
     9001 gtataggtgt cttgcaaact aacgtcgggc gacacgtagt tgttttctgt taaaattgat
     9061 gaatacgaat tgtgtttaat atcccctaca atgccaccat acagataaaa taaatcggga
     9121 actaaagcag ccttacctac aatgtttgca gcaccgctta caggacgttg ggtggctcca
     9181 ataattgcaa ataaacttgc acccatgctg atgtcccagt ttttgccaac aaaaatgtag
     9241 tagggagtta atttaatgac actcgtattg ccaaaatcag tctctttaat agcatagttt
     9301 gcacttagct caagtggttt cttgtaaaaa taattatcca cgtcaaccga aacgcctatt
     9361 cttcctgttt ctagcgaccc actaaggttt gtaccaacat aaaaattatg ttcttctact
     9421 gcaatatgtg atgcaaaata ctgataagaa aggttgctat tgtgctgaaa agaagattgt
     9481 tggtttgtag aaaataggcg aaaattagta cccgcttttg taaaacttcc aatgcctgat
     9541 tcgagcaaac tgtcggcttt aatttttact atttcgcgtt gggtatctcc cacccacaaa
     9601 gaggtatcta tgtttgattt tccataataa tcaaaccctt tatgcaaaaa atgaatacca
     9661 gaaacaagac gagaattacg aaattgattt tcaaatgtaa atcctgctcg tgtggtagca
     9721 aacatagctc gcgtaggttc attatcctca atttttaatt ttccccacgc agattgatgt
     9781 gatacattaa ggtcaagtaa ataggtatct ccttgtaaaa gaggataata aaaatctcct
     9841 agtaattgcc aatgactacc aatacccgct ctaaatactc cgttttttat ttctcccaat
     9901 tgtgtgtctt tatacgtatc ggttgctaaa gggttaaaac ttttgtcaac tgttgccgga
     9961 gaagagctaa tggtatatgt aatatccttt ttatcaatag tatattcttc tgttttaggg
    10021 acggtattaa ttttcttggc atctattgct gtagggttgt attctctttc taaagttaca
    10081 tctcgtgaaa cgtttgattg cgaaaaactc aacgatgtat tggctaaaaa tagtaccaaa
    10141 ggtagtatat agattattct tttcatacaa ttttgtttat aggattgtga ttacacacca
    10201 ttccggctta ctaactttat taattattca ctttttcatt ctcaatttta gcaatctctt
    10261 ccaaacgagt attgatttct gatgctattt ctacatcgtc tttgggataa ttacttttta
    10321 aacttaataa gtattgtttt gcttcaaaat aattatcctg ttccatatat acgtcggcga
    10381 gcaatataaa ggctttggct aaccatttgt gatgcggagt tcccttatct ataaaatcaa
    10441 acacttcttt ttccgctgat tcatattgtt tggctaggta gtaatgtgta gcaagtaagt
    10501 atttagcttc tgctccaaag gcatttcgtg tatctttagc taagatttgc atatctggca
    10561 gtgcattttg tgctttattt agctctaaat aactcaacat acgtttgtgt tgtgcttcgc
    10621 gaagcaattc aaggttgttg ctatgcaagg atataaaatt agtggcatac gtaattgttt
    10681 catcatagcg tttcaaaata tacgatgctc tcatcagtcc gatacgtgct accattttat
    10741 tttcgctcga accaatcgct tcgagttgtt taaaatattc cattgattct gcatattgcc
    10801 ccaaatcata gcttatttct gccgcccttg ctaaggtttc ttcctgattt ggattctctt
    10861 tatcagatag cagtgcttta tataaaggga gggcttttgc aggctgtttt tgtgtataat
    10921 aacaacttgc taacaaatag cgagcagtag tactataacg ccctttggga taggtatcta
    10981 aatatttctc aaaacttgaa attgcttgtg gtatttcgtt gcgcgaatag acacgctgag
    11041 ccgctctaaa caataatgag tcttctctcg aaggagctat ttggatagag ccttttccca
    11101 aagcattaac aaactcggta tattcactta cacgattatc ttctaccaaa atattctcaa
    11161 tagcttccaa cgatattcta gcctcttcgc ttgagggata ggtacgtaca agttgtttgt
    11221 acgactctaa ggctgcatct gtttttccat cattgtattg caacatcgca atttgtaagc
    11281 gtgcttgtcg tataagctga tgattacggg agaatttctt ttcaatttct gcaaaggttt
    11341 caatagcctt atcgttttct tttaatacca cgtaggtttt accaatttca atgtaagcgt
    11401 catctaccca ttctgattga ggatacgtag taattaaatt tcgtaaggtt gatattttct
    11461 cattatgatt tcgttctaat ccagcaacaa atcctttttg gaatgtagca taatcagctc
    11521 caggtaattg agcccctgtt gctatggctt gattataata acgttttgcc tctgcaaatt
    11581 gtctgttttg gtaataagaa tcgccaatac gattattggc atccaacacc atgaggttgt
    11641 tgtctggttt taatgcaatg tatcgtttaa accatgttac agcatctgtg tagttcttca
    11701 gtaaatagta ggtgtacccc atactataat gagcaatatc gtattctgct agtttttgtg
    11761 ctttaggtga ttgtaaaaag gtttgaaaat tcttttcagc ttgggtataa tatccagtat
    11821 tcaacaaact ttctccatac cagtaaaatg aacgagtttt aaagtccgca ttgtatgatt
    11881 tacccttcaa tattttctca aaatgcgatg ttgcttgttg gtaattgtta ttggcaacag
    11941 ccactatacc catattgaat aaaatacgct cttcagcttc tttaattttt ttattgtcag
    12001 ttttcaggtt ctgaatagaa cgatatgctt ctccataatt ttttgtagtt aaatagctgt
    12061 ttgctaggta ttcatatact ttgtctttgc gtttggatga ggggaattcg gttaaaaaac
    12121 gctcaaaagc aactactgat tcattaaatg gagaaaatga ctgttcgtaa ctaactaccg
    12181 caaaattata caaggcttct tcttgtatgc ctttatcaaa ggtaaaacga gaagctgttt
    12241 caaaagctag tcgtgcattt ttcttatcgt ttagcttgac atatcccatt cccaagtata
    12301 ggtaactatt ttgtgctaat aaatcttctc tgtttgttgt tttagacaag tttgcaatag
    12361 ctttttcata ttcacccacg ttgtaatgag ctatccctaa catataaaaa tcttcacgcg
    12421 aaattcgttt tacttgttgc tcatactgag taaggtaatg tatgcttgag ctataatctt
    12481 tggcaagaaa actacattcg cccatcatcc gtaatacttc attacgtcga ttgtttttag
    12541 ggtatttctt tataatttct tttccgtaat ttaatacttt atcacaattt ccttgctgat
    12601 aatatagttg ggcaataaaa aatggagcac tagtatgaaa ttcaggatga ttttcgacgc
    12661 gtaaaaaatg agtaagagca ctatcaaaat tgcctaatcg ttgctctgca taagcaaaat
    12721 aataggtagc tgcatcgtaa taaggcgtat tcgtgcctct gcggagtact tgctgaaatt
    12781 caaaacgaga tttttcaaaa ttttcttgtt gatggtatgc atagcctttg tagaattgca
    12841 atttaactag atccgattcg cgtaatctat ctgtttttat gctctcaaaa cgacgaatgg
    12901 cttgcttaaa tctaccagat tcgtactcta aaatgccaat atatagattc gtaagttcta
    12961 cgtctgttgc ataagggtat tttgtctgat atgcctcaag tttaatttgt gcgtcatttt
    13021 ttctaagttc aaatgctgaa cgagcaatgt aatactccgc ttcacgcacc atttcgtcat
    13081 tgtctcgttg gtcaaattgt aaaaactctt caaaatatcg tgccgatgca gcataattat
    13141 tattgtagaa aaaggactta ccgtctgcaa aaagttcagc agcagtagtg ttaaaaggtg
    13201 atttttgact aaaaagagga gtaacggtta aactaaataa aagacaaaaa atggaaatgt
    13261 gtaagtattt cataagtgtt cgattttttc tgaatcaatg tataacgcta gaacgaataa
    13321 aaaatcagat tattacttgc aaaggtataa attatatttg aatttaaaaa cataaaaatc
    13381 ctcaatcaat ttacgttttt tgtgtacctt tgtgttttaa tcaattcgtc attcattatg
    13441 agaacttaca ccaacgtaaa agatttagga aatttgcaac aagcattaaa tgaggctttt
    13501 gaagtaaaaa ataat
//
LOCUS       DAOSRP010000051        12785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4929_length_12785_cov_5.638885, whole genome shotgun sequence.
ACCESSION   DAOSRP010000051 DAOSRP010000000
VERSION     DAOSRP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12785
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_12785_cov_5.638885"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLJ82_04500"
     CDS             <1..602
                     /locus_tag="PLJ82_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR40798.1"
                     /translation="FINYMIHYAKDTDVLFLGDLNSYKYEDPITTLRNGGLEDLLEHF
                     QGNDAYSYVYGGKAGYLDYALANSSMLRQATGASPWHLNSDEPSQLSYDNATYYQPNM
                     YRYSDHDPIVVGLKLTPQAPFEPLDKIQLFHDLTSKHIIVTRAKNHQVSIIGIDGRFA
                     HFHSIESDTETINTNMLPAGVYVVKITGTKEETFKFIQL"
     gene            complement(612..989)
                     /locus_tag="PLJ82_04505"
     CDS             complement(612..989)
                     /locus_tag="PLJ82_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOR40799.1"
                     /translation="MKRIISTTKAPAAIGPYSQAIEVNDTLYISGQIPVNPENGKVDA
                     LTIEQQAEQVLQNIGAILQAAGYGYSDVVKSTIFITDMKNFSAVNEVYKKYYTEKFPA
                     RSTVAVKELPLGVMVEIETIAVK"
     gene            complement(998..2182)
                     /locus_tag="PLJ82_04510"
     CDS             complement(998..2182)
                     /locus_tag="PLJ82_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOR40800.1"
                     /translation="MKQYNFDEVIDRTNTSAYKLVLMEKYFGSNDAIPLWVADMDFRT
                     PDFILDAIKNRCDSGVLGYTLPSADYTTSIAKWLKYKHDWEVSEKFVAFAPGVVTGLA
                     LAIRAFTNPHDKILVQTPVYPPFLSLPNEHNRELVINPLQFHSNTFEIDFFDFEKKLA
                     SGVRMFILCHPHNPGGRVWNADELMRMAKLCIQYNVLMVSDEIHADLMLWGKQHIPLA
                     KLLPEIAQHTITFMAPSKTFNIPGLASAFFVAENPQLFAKMQQETANFHLNMGNVFGC
                     MATEVAYNEGKEWLNQAIHYIEQNILFVRNYIEQHIPIIQVVVPDASFLVWLDCRKMG
                     LTSKELKEFMVHKAKVIMNDGATFGQGGEGFQRLNVACPRSVLQQALQQIETAINEMF
                     TK"
     gene            complement(2197..3522)
                     /locus_tag="PLJ82_04515"
     CDS             complement(2197..3522)
                     /locus_tag="PLJ82_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOR40801.1"
                     /translation="MAVKKNFVLDTNVILHDHKCIYKFQEHDIYLPIVVLEELDKFKK
                     GNEEINFNARQFARELDLVTDNDLFTKGASLGEGLGRLFIVLGVPFSDTMQVSFMEKT
                     PDHRILAITQALQEKNKKQKTILVSKDINLRMKARSLGVLAEDYFNDKITSKDIFDKE
                     HETFDNIDSDVIDRIYSTAAGVPLAQFEFQSDVQPNDYFVLKSERNSVLARYNPFFET
                     VVKVRKEKAFGIEPRNAEQSFAINALLDDEIKLVCITGKAGTGKTLLALAAALEKNNE
                     YKQILLARPIVALANKDIGFLPGNQKEKIAPYMQPLFDNLNVIKHQFAVESREQLLIK
                     EMLVSEKLVIEALAFIRGRSLSDAYIIVDEAQNLTPHEIKTIITRAGENTKVVFTGDV
                     QQIDSPYLDMFSNGLVYAIDKMKGQHIFAHVNLVKGERSYLSELASNLL"
     gene            3658..4932
                     /locus_tag="PLJ82_04520"
     CDS             3658..4932
                     /locus_tag="PLJ82_04520"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOR40802.1"
                     /translation="MTYEQTLQYLYEKTPLYQHIGNKAYKPGLDNIQQLDTHFEHPHT
                     AFKTIHVGGTNGKGSVSHSLAAVLQSNGYKVGLYTSPHLQDFAERIRVNGEKISKNYI
                     IDFVSNSQKYIEQMSLSFFELTTMMALCYFKEQKVDYAIIEVGLGGRLDSTNIITPIA
                     SVITNISFDHTYLLGNTLKEIATEKAGIIKQNTPIIIGEKQEKTNAVFKNKAKEMNAP
                     IFFAEEMYQLSQLKEEAEHTVYSIDNFGTIACQLRGWCQQKNAITILSAIQVLQHMCK
                     IAISEKAIHHGLANVTSLTGLLGRWQVMQYHPTMIADTAHNEAGLKWIIKQLTAMTYE
                     RLHIVLGLADDKDTDKILNLLPKEATYYFTQASIKRALPASVLQQEGKKLGLQGSCYN
                     SVMEAKKAALKDANDKDVIYIGGSTFVVAEAI"
     gene            5025..6269
                     /locus_tag="PLJ82_04525"
     CDS             5025..6269
                     /locus_tag="PLJ82_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOR40803.1"
                     /translation="MIKKLLIIAIIFILSSAYTHANFITLSEKSTVSLITCSAGEAIY
                     ELFGHTAIRVVDTENQLDIVFNYGLFSFDEPNFIGRFVRGKTDYEVGIQGFQDFVLSY
                     AMRNSSVKEQILNFTPEEKQILWEKLNTDILPENKKYRYNFIFNNCATRPRDIIERAS
                     TGHVIYPYNISPMTFREAIGLYTKDAKWSKFGFDLCLGMNADKTATETDLHFLPEMLS
                     ESLAQTVVEYNASKKEKLTQVPTYIIQANEKESISTDSHTISPLSVSWLLFAIVLTIT
                     IFAYIKKKKLYLLDGFLFLIAGSVGSLLLFLALFSKHPFTDNNFNIVWLNPFWFVPFV
                     LFIIPIPRKKDVLIAFYIAVSIVLTIFMLSIWAIPQYFNPAFIPLVLVLLTRVVSYVF
                     YWGYKKDKTGSYYKRKKRTSNK"
     gene            complement(6328..6528)
                     /locus_tag="PLJ82_04530"
     CDS             complement(6328..6528)
                     /locus_tag="PLJ82_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOR40804.1"
                     /translation="MKHVVGTNAGVVWGALAEANGTLEFKELKKLTKLKEKDLYLAFG
                     WLLKEGKLVFREEEKDLYISLV"
     gene            complement(6625..7983)
                     /locus_tag="PLJ82_04535"
     CDS             complement(6625..7983)
                     /locus_tag="PLJ82_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR40805.1"
                     /translation="MYLSQYIPFYKKNLKIAIPIILSQLGGGLVTVVDTIMVGHLGTV
                     ELAAASFANSIFLIGFVFSIGLTMAITPLVGKAYAEKKGKEIVELFQNSFLFVIIMAV
                     LIMIILLLAYFFMDYMGQQITVVELAKPYYILLIISLFPFLMFSVYKQFVEGLGNTAV
                     AMYITIIANIINIVFNYLLIFGKFGFPAWGLVGAGIATLLSRIVMWMLFVVVVRYNLT
                     WRSYVQSFSWSLFNTKTIKRLSSVGFPIGGQMFLEISAFGIATIMVGWLGDVSLAAHQ
                     IAQIMAHVSFMGILGLAAAGTVRVSHEYGHKNIYNLQMSITASRHLVLVMTCFTGIIL
                     LSMRNYIPLIFTTDSTVIEIASTLIVLVGLFQISDGMQAVGAGVLRGLTDVNVMVRYA
                     FFAYICINIPVAYLLAFVVGLREQGIWIGFIFGLSCAAILFYLRYKKKIKLLQTTLNK
                     IS"
     gene            complement(8091..8906)
                     /locus_tag="PLJ82_04540"
     CDS             complement(8091..8906)
                     /locus_tag="PLJ82_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOR40806.1"
                     /translation="MERMEVLVDKLMSSGFEIGKNVVIALVIFVVGRYLISFVNKLVR
                     AMMERRQLDPAIRSFLGSMINIVLMVLLLISVVGALGVQTTSFAALLASAGIAVGMAL
                     SGNMQNFAGGLMILFFKPYKIGDFIETQNVMGTVKEIQIFHTILTTPDNKIIFIPNGP
                     LSNGVLINFSHQLTRRIEWTFGVEYGTDLEKVKQILTSLIEKDSRILLEPTYLIALNT
                     LADSSVNVVVRAWTKNEDYWSVYFEMNQSVYTTFNKEGINFPFPQLVVHHNKE"
     gene            complement(8991..10454)
                     /locus_tag="PLJ82_04545"
     CDS             complement(8991..10454)
                     /locus_tag="PLJ82_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOR40807.1"
                     /translation="MNNQSSVNIKFASGEQLPLELHKVRIVQKLHLVPIERRLQALEE
                     GGYNTFQLGTLDVFLDMLTDSGTNAMSDNQLAAMMRADDAYAGSQSFVRLQKAVEEVL
                     GQKYLLPVHQGRAAENILGKAYVKKGSLVPMNYHFTTALAHITENGGSIAELLYPEAY
                     KIDSECLFKGNMNLELLEETIQKVGVENVPYIRMEASTNLIGGQPFSVQNLRDVKAIA
                     KKYGIRLVLDASLLGENAYLVKQREDEFKQKSMAEVILTMTSLADIIYFSARKLSSSR
                     GGGICTNNEAVYKELEPLVPLFEGFLTYGGISVREVEAMAVGLYETLDETMISQSPEF
                     IKYFVYDAKSKGIPMITPPGVLGAHVDAMSFCSHIPQTQYPAGALAAAFFLISGIRGM
                     ERGSVSNQRDENGNETYADMELLRLAIPRRVFTLSQIKYAIDRLAWLHKNRELIGGLK
                     FVYEPPVLRFFMGKLAPVSDWPQKLIAKFKEDFGDSL"
     gene            complement(10550..>12785)
                     /locus_tag="PLJ82_04550"
     CDS             complement(10550..>12785)
                     /locus_tag="PLJ82_04550"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOR40808.1"
                     /translation="HQLRKLGASCDWSRTAFTMDEERSISVLKVFCDLFEKGKIYRGV
                     RMVNWDPQALTALSDEEVIFKEQQGKLYYLKYKVVGEDAYAVVATTRPETIMGDTAMC
                     IHPNDAKNAFLKGKKVIVPLINREIPVIEDEYVDAEFGTGCLKVTPAHDVNDYMLGEK
                     YNLPSIDIFNDNGTLNEHGCQYTGMDRFDVRNKIEQDLLAAGLLEKVEPYTNKVGYSE
                     RTNVPIEPKLSMQWFLKMEDIAKPALDAVMTDDVKFYPAKYKNTYRHWMENIKDWCIS
                     RQLWWGHRIPAYYLPEGGYVVALTEQEALEKAQKMNPSLTLADLRQDEDCLDTWFSSW
                     LWPISLFNGITEPNNEEIKYYYPTSDLVTGPDIIFFWVARMIMAGYEYENKQPFNHVY
                     FTGIVRDKLGRKMSKSLGNSPDPLELIERFGADGVRMGMMLAAPAGNDILFDESLCEQ
                     GRNFNNKIWNAFRLVKGWEVADIPQPESAGVAIKWFKAQLAKTITEVDDLFSKYRISE
                     ALMAVYKLFWDEFSAWYLEMVKPAYQQPIDKVTYEATLQFFEELLLVLHPFMPFITEE
                     LWQSIYTRQDGESIVVQLQSKASEYDEELIVRFEKIKEIIAGIRTIRVQKNIPNKETL
                     PLQIVGEHNSFFDLILLKLGNLRSIEHVNEKTDTSVAFLVGTTEYAIVLGNLINIEDE
                     IKKIETEIRYLEGFLQSVVNKLSNERFVANAKPEVVDAERKKQLDAESKISTLREALQ
                     KFQA"
BASE COUNT         4277 a         2490 c         1922 g         4096 t
ORIGIN      
        1 cgtttatcaa ttatatgatt cattatgcca aagatacaga cgttctattt cttggtgact
       61 taaattctta caagtacgaa gatccaatca ctaccttacg caatggagga ttggaagatt
      121 tactagagca ttttcaagga aacgatgctt attcgtatgt gtatggagga aaagcaggtt
      181 atttggatta tgcactagca aacagcagta tgttgcgtca agcaactgga gctagtccct
      241 ggcacttaaa tagcgatgag ccatcgcaat tgtcatatga taatgccacc tattatcaac
      301 caaatatgta caggtattcc gaccacgacc ctattgtagt tgggttgaag ctaacaccac
      361 aagctccatt tgaacccttg gataaaattc aattatttca cgatttgaca tccaaacata
      421 tcattgtaac acgtgcaaaa aatcaccaag tgtctattat tggtatcgat ggacgttttg
      481 ctcacttcca ctcaattgaa tcggatacag aaacaataaa caccaacatg ttgccagctg
      541 gtgtttatgt tgtaaaaatc acaggaacga aagaagaaac gtttaagttt attcaattat
      601 agaatactac cctattttac ggcaatcgtt tcaatttcta ccattacccc caatggtagt
      661 tcttttacag ctacagttga acgagcagga aatttttcag tataatattt tttgtacact
      721 tcgtttacag ccgaaaaatt cttcatgtct gttataaaaa tagtggattt tactacatct
      781 gaatagccgt agccagctgc ttgcaaaatg gctccaatat tttgaagtac ttgctctgct
      841 tgttgctcta ttgtaagagc atcaacttta ccattttctg ggtttacagg tatttgacca
      901 gaaatataca aggtatcatt tacttcaata gcttgactgt aaggacctat tgctgctgga
      961 gcttttgttg ttgaaattat tcgtttcata ttttttatta tttagtaaac atttcgttaa
     1021 tcgcagtttc tatctgctga agagcttgtt gtaatacact acgtggacat gctacattca
     1081 gtcgctgaaa accctctcct ccttgaccaa aagtagcccc atcattcatt attacctttg
     1141 ctttatgaac cataaattct tttaattctt tggaagttaa acccattttt ctgcaatcaa
     1201 gccatactaa aaaggatgca tcgggcacaa ctacttgtat gataggaata tgctgttcaa
     1261 tataattgcg tacaaatagt atgttttgtt caatataatg tatagcttgg tttaaccatt
     1321 ctttcccttc attgtaagcc acttctgttg ccatacagcc aaatacattt cccatattga
     1381 gatgaaaatt agctgtttct tgttgcatct tggcaaaaag ttgcggattc tcagctacaa
     1441 aaaaagcact agctaaacca ggtatgttaa atgttttact aggtgccatg aaagtaatgg
     1501 tatgttgagc aatttcgggc aataatttgg ctaaaggaat atgttgttta ccccacagca
     1561 tcaaatctgc atgtatttca tccgacacca ttaacacatt gtactgaata cacaatttag
     1621 ccatacgcat caactcgtca gcattccaca ctcttcctcc aggattgtgt ggatgacata
     1681 aaataaacat gcgaacacca gaggctaatt tcttttcaaa atcaaaaaaa tctatttcaa
     1741 aggtattcga atgaaattgt aagggattga ttactaattc tctattatgc tcattaggta
     1801 aactcaaaaa aggtggatac acaggtgttt gtaccaatat tttatcatga gggttggtaa
     1861 aagcacgaat agctaacgcc aaaccagtaa ctacgccagg agcaaaagca acaaattttt
     1921 cagagacctc ccaatcatgt ttatatttca accattttgc tattgacgtt gtataatctg
     1981 ccgaaggcaa ggtgtagcca agcaccccgc tatcgcagcg attttttatc gcatcaagaa
     2041 taaaatccgg tgtacgaaaa tccatatctg ccacccataa tggtatggca tcattagagc
     2101 caaaatactt ttccatcaac accaacttat acgcagaggt gtttgttcta tctattactt
     2161 cgtcaaaatt gtattgtttc attcttccac atatagttat agcaaattac ttgctaattc
     2221 agacaaataa ctacgttctc ctttcacaag attaacatgt gcaaaaatat gttgcccttt
     2281 cattttatca atggcatata ctaatccatt agaaaacata tccaaatacg gagaatctat
     2341 ctgttgcaca tcgccagtaa ataccacttt tgtattttca cctgcacggg taatgattgt
     2401 tttaatttcg tgtggggtta agttttgtgc ctcatctaca ataatatatg catcagacag
     2461 acttctacct cttataaatg caagagcttc aatcactaat ttttctgaca ccaacatttc
     2521 ttttatcagc aattgctccc tactttctac ggcaaattga tgtttaatta cgttgaggtt
     2581 atcaaacaac ggttgcatat acggtgcaat tttttctttc tggttgcctg gcaaaaaacc
     2641 aatatctttg ttagccaaag ccacaatcgg acgtgctaac aggatttgtt tgtactcatt
     2701 gtttttctcc aaggctgcgg caagtgctaa caatgttttt ccagtacctg cttttcctgt
     2761 aatacaaacg agtttgattt catcgtctaa caaggcatta atagcaaaac tttgttcagc
     2821 attgcgtggt tcaatcccaa atgctttttc tttcctcact tttacaacgg tttcaaaaaa
     2881 tggattgtaa cgagcaagta ccgaattacg ttcacttttc aacacaaaat aatcgttggg
     2941 ttgcacatca ctctgaaatt caaattgtgc taaaggtaca ccagcagcgg tactgtaaat
     3001 tctatcaatc acatcgctat caatattatc aaacgtttcg tgctctttgt caaaaatatc
     3061 tttgctcgtt attttatcgt taaaataatc ttctgccaaa acgcccaacg aacgagcctt
     3121 catacgcaaa ttgatatctt tacttactag aatggttttt tgctttttat ttttttcttg
     3181 taaggcttgc gtaatggcta aaatacgatg atctggcgtt ttctccataa aagacacctg
     3241 cattgtatcg gaaaaaggta cacccaacac aataaacaaa cgtcctaaac cctctcccaa
     3301 agaagctcct ttggtaaata aatcattatc agtaaccaaa tcaagttcgc gggcaaactg
     3361 ccgagcattg aaattaattt cctcgtttcc ttttttaaac ttatctaact cttctaacac
     3421 aacaattggt aaataaatgt catgttcttg aaatttatag atacatttgt gatcgtgtaa
     3481 aattacattg gtatctaata caaaattctt ttttactgcc atagtattcg tcttttgggg
     3541 ttgattttgt taaaaacacg tgtaaatgta gtataaaaaa aagataaaac gtaattttac
     3601 atcgtctttt tataaacatt aataaagtca atatactgtt acaattatca actaactatg
     3661 acatacgaac aaaccctaca atacctgtac gaaaaaacac ctctatatca acacattggc
     3721 aataaagcct acaaaccggg attagataat attcaacaac tcgacaccca ttttgaacat
     3781 cctcatactg ctttcaaaac catacatgtg ggcggcacta atggcaaagg ctccgtttcg
     3841 cattcattag cagccgtgct acaatcgaat ggctataagg tagggttata tacttctccg
     3901 catttacaag attttgccga acgaatacgt gtaaatggag aaaagattag taaaaactat
     3961 attattgatt ttgtaagtaa ctcgcagaaa tatatcgaac aaatgagcct ttcatttttt
     4021 gaactcacca caatgatggc attgtgctat tttaaggaac aaaaggtgga ttatgctatc
     4081 atagaagtag gtcttggagg tagattagat agcacaaata tcatcactcc tatagcaagt
     4141 gtcattacaa atattagttt cgatcatacc tatttgcttg gcaacacatt gaaagagatt
     4201 gctacagaaa aagcaggcat tatcaaacaa aacacaccca tcataattgg agagaaacaa
     4261 gaaaaaacaa atgcagtatt taagaataag gcaaaagaaa tgaatgcccc tatatttttt
     4321 gccgaagaga tgtaccaact ttctcaatta aaagaagaag ctgaacatac ggtatatagt
     4381 attgataatt ttggtacaat tgcatgtcaa ttgagaggtt ggtgccaaca aaaaaacgcc
     4441 atcaccatct taagtgctat tcaggtactc caacatatgt gtaagattgc aatttcagaa
     4501 aaggcaatac accatggact agctaacgtt acgtctttaa caggtttatt gggaagatgg
     4561 caagtgatgc agtaccaccc aacgatgata gctgacacag cacataacga agctgggtta
     4621 aagtggataa taaaacaatt aacggcaatg acatacgaac gcttacatat tgtattgggc
     4681 ttagctgatg acaaagacac ggataaaata ctcaatttat tgccaaaaga agctacctac
     4741 tattttaccc aagcatctat taaacgagcg ttgcccgcta gtgtattgca acaagaaggt
     4801 aaaaaactcg gattacaagg ctcttgctac aacagcgtaa tggaggcaaa aaaagctgca
     4861 ctaaaagacg ctaatgataa ggatgtaatt tatatcggag gaagcacatt tgtagtagct
     4921 gaggcaattt aacatactat ataaataaaa aacaactaaa aatatttgaa gaaaaagata
     4981 aaagttgtac ttttacacct taaccctaaa aacaggtagt taatatgatt aaaaaactcc
     5041 ttattatagc catcattttt attctatctt cggcttatac tcatgctaat tttattacac
     5101 taagcgaaaa gagcactgta tctctcatta catgttctgc gggtgaagct atttacgaac
     5161 tttttggaca taccgctata cgtgtagttg atacagaaaa ccaattggat attgttttta
     5221 attatggttt attttctttt gacgaaccaa actttattgg tcgttttgtt agagggaaaa
     5281 cagattacga ggttggcatt caaggatttc aagattttgt actgagctat gccatgcgta
     5341 actcaagtgt aaaggaacag attctcaatt ttaccccaga agaaaaacaa attctttggg
     5401 aaaaattaaa tacggatata ttgcccgaaa ataaaaagta taggtataat tttattttta
     5461 ataactgtgc tactcgtcca cgagatatca ttgaacgtgc cagtacaggt catgttatct
     5521 atccatacaa cataagccct atgacattta gggaagctat tggactttat acaaaagatg
     5581 ctaaatggtc aaaatttgga tttgacctct gtttaggtat gaatgcagat aaaacagcta
     5641 ctgaaactga tttacatttt ttaccagaaa tgttatcaga gtcactagca cagacagtgg
     5701 tggaatataa cgcaagtaaa aaagaaaaat taacccaagt acctacttat attatacaag
     5761 caaatgaaaa agaatctata tccacagatt cacataccat ttctccatta agtgtgtctt
     5821 ggttattatt tgctatcgta ttaactataa ccatattcgc atatataaaa aagaaaaaat
     5881 tatacctatt agatggcttt ctatttttaa ttgcaggctc tgtggggtca ttattgttgt
     5941 tcttagcatt attctcaaaa catcctttta ccgataacaa cttcaatatt gtatggttga
     6001 atcccttttg gtttgttccc tttgtattgt tcattatacc tattccgcgg aaaaaagatg
     6061 tattgattgc tttttatatt gctgtgagca ttgtacttac catatttatg ctatctatat
     6121 gggcaattcc ccaatacttt aatccagctt tcatccctct tgtattagta ttactcacaa
     6181 gagttgtttc gtatgtcttt tattggggtt ataaaaaaga taaaacagga agttattata
     6241 aacgaaaaaa aagaacatca aacaaataaa catcaaagag gctatctatt aattagatag
     6301 cctctttgat gtataacaga taagaatcta taccaaagaa atatatagat ctttttcttc
     6361 ttccctaaac accaattttc cttctttaag caaccaacca aaagctagat atagatcttt
     6421 ttctttaagt tttgttagct tttttaattc cttaaactca agtgtaccat tagcttctgc
     6481 taaagcaccc caaacgacac ctgcatttgt tcctactacg tgtttcataa cttatacatt
     6541 ttatgtggtt agacaatagt acaaaagtaa cctaaaatat tgtaaacaca ttagccaatg
     6601 tgttgattta ataaaaactt aaaactacga aattttattt agggtagttt gtaacaattt
     6661 tatcttcttt ttgtaacgta aataaaataa tatagctgca caactcaagc caaatataaa
     6721 acctatccaa ataccttgtt ctctgagtcc tactacaaaa gccaatagat aagcaactgg
     6781 aatattaata catatataag caaaaaaagc atatcttacc attacattta catctgttag
     6841 tccacgcaaa acgcctgctc ctactgcttg cataccatct gatatttgaa acaatccaac
     6901 taatacaatt aatgtagaag caatctcaat tacagtagaa tctgtggtaa agattaatgg
     6961 aatatagttt cgcattgata gtaaaattat tcccgtaaaa caagtcataa caagtaccaa
     7021 atgacgtgag gcagtaattg acatttgtag attatagata tttttgtgtc catactcatg
     7081 actaacacgc actgttccag cggcagctaa tcctaatatc cccataaaag aaacatgagc
     7141 cataatttgt gcaatctgat gtgcagctaa agaaacatca cctagccatc caaccataat
     7201 agtggctatc ccaaaggcgg aaatttctaa aaacatctga ccaccaatcg gaaacccaac
     7261 agaagaaaga cgtttaatcg tttttgtatt aaacaacgac catgaaaaag attgtacata
     7321 actacgccaa gtaagattat atcggacaac aacaacaaag agcatccaca tcacaatacg
     7381 ggataataat gtagcaatac ctgctcccac taaaccccaa gctggaaatc caaatttacc
     7441 aaatattaaa agataattaa atacaatatt tattatattg gcgataatgg ttatatacat
     7501 agcaacagct gtatttccta agccctctac aaattgtttg taaacagaaa acattaaaaa
     7561 agggaataaa cttataatta gtaagatata atatggtttt gctaattcaa ctacagtaat
     7621 ttgttgaccc atataatcca taaaaaaata ggcaagcaac agtattatca taattaaaac
     7681 agccataatg atgacaaaaa gaaaagaatt ctgaaacaat tcaacaattt cttttccctt
     7741 tttttctgca taagcttttc ctactaatgg ggttattgcc attgttaaac caatagaaaa
     7801 aacaaagcca attaaaaaaa tagaatttgc aaatgaagcc gcagctagtt ctactgtacc
     7861 taaatgaccc accataatgg tatcaactac agttaccaat cctcctccaa gttgtgaaag
     7921 aatgatagga attgcaattt ttaaattttt cttataaaaa ggaatatact ggctaagata
     7981 catatgttta ttaattctgt aatagaaacg caaagttaat cattatactg caaaaaaata
     8041 gagagtagaa acacaaccct ctatttttac atattcctaa caaatacacc ttactcctta
     8101 ttgtgatgaa caaccaattg tggaaatgga aaattaattc cttctttatt aaatgtagta
     8161 tatactgatt gattcatttc aaaatatact gaccaataat cttcgttctt agtccaagct
     8221 cttacaacca cattcacact actatcagct aatgtattta gtgcaataag atatgttggt
     8281 tcaagcaata tccttgaatc tttttctatt aaagaagtta atatttgttt cactttttca
     8341 aggtcagtac catattccac tccaaatgtc cattcaattc tacgtgttaa ctgatggcta
     8401 aaatttatta gcactccatt gcttagaggt ccgttaggaa taaaaattat cttattatca
     8461 ggagttgtta gaatagtatg aaaaatctga atttctttta cagtccccat tacattttgg
     8521 gtttcaataa aatcgccaat tttgtaaggt ttaaaaaata atatcatcaa tccacctgca
     8581 aagttttgca tatttccgct caaagccata cccacagcaa tacctgctga tgctaataaa
     8641 gctgcaaaag aggttgtttg tacaccaaga gcacctacta cagatattag taatagtacc
     8701 atcaagacaa tattaatcat acttcccaaa aaactgcgga tggctgggtc taactgacgt
     8761 ctttccatca tggctctcac taatttattc acaaatgaaa ttaggtaacg cccaacaaca
     8821 aaaatgacta gtgctatcac aacatttttt ccaatttcaa aacctgaact catcaatttg
     8881 tctactaaaa cttccattct ttccataaga aaaaaaaatt aaaattgtta tacaaaaaaa
     8941 aatctactga ggaaagaatc ctcagtagac aagatatcac aacaaacagc ttataaacta
     9001 tcgccaaaat cttccttaaa ttttgcaatc agtttttgag gccaatcaga tacaggagct
     9061 aatttcccca taaagaaacg taaaactggt ggttcataca caaattttaa tccaccaatt
     9121 aactctctat ttttgtgtaa ccaagccaat cggtcaattg catattttat ttgagacaag
     9181 gtaaatactc tacgaggaat agccaaacgt aataattcca tatcagcata tgtttcattg
     9241 ccattttcat ctcgttgatt agacacagac cctctctcca tacctctaat accagagatt
     9301 aagaaaaatg cagcggctaa agctcccgca gggtattgtg tttgaggaat atgtgaacaa
     9361 aaactcattg catctacgtg agcccctaat actccaggag gagtaatcat tggtatgcct
     9421 ttgctctttg catcatacac aaaatattta ataaattcag gactttgcga aatcattgtt
     9481 tcgtctaatg tttcgtataa tcctactgcc atagcctcta cttcacgaac agaaataccg
     9541 ccataggtta agaacccttc aaacaaaggc actaatggtt caagctcttt atatacagct
     9601 tcgttatttg tgcaaatacc tcctcctctt gatgaactta acttacgagc cgagaaataa
     9661 ataatatctg ctaaagaagt catagtaaga ataacctcag ccattgattt ttgtttaaat
     9721 tcatcctctc tttgttttac caaataagca ttctctccca ataatgatgc atctaaaacc
     9781 aatcgtatac catatttttt tgcaattgct tttacatctc gcaaattttg cacagaaaaa
     9841 ggttgccccc caattaaatt ggtagaagct tccatacgaa tataaggcac attctcaaca
     9901 cctacttttt ggatggtttc ttctaataat tccaaattca tatttccttt aaatagacat
     9961 tcagaatcaa ttttataggc ttctggataa agcaactcag caattgaacc cccattttct
    10021 gtaatatgtg ctaatgctgt cgtaaagtgg taattcattg gcaccaatga tccttttttt
    10081 acgtatgctt ttcctaaaat attttcggca gcacgacctt gatgcactgg taacaaatac
    10141 ttttgtccca atacttcttc aacggctttt tgtaaacgaa caaaactttg agagcctgcg
    10201 taagcatcgt cagcacgcat cattgcggct aactgattat cgctcatagc attcgtccca
    10261 ctatctgtca acatgtctaa aaaaacatct aaagttccta attgaaaagt attgtatcct
    10321 ccctcttcaa gagcctgtaa acgacgttca atgggtacta aatgtaattt ttgcacgata
    10381 cgcactttgt gcaattccaa tggtaattgt tcgccactgg caaatttaat gttaacagaa
    10441 gattgattgt tcatgtgtgt aattagttta atagtaattg attatataaa gtttttttta
    10501 agtagtacaa agatactaaa attatagaat atgccataac ctatacattt tatgcctgaa
    10561 atttttgtaa tgcctcacgt aaagtggaga ttttactttc tgcgtctagt tgttttttgc
    10621 gttctgcgtc taccacttcg ggtttagcat ttgctacaaa tctttcattg ctaagtttat
    10681 taactacaga ttgcaaaaat ccttctaaat agcgtatttc tgtttcaatt tttttgattt
    10741 catcttcaat atttattaaa ttccccaaca caatggcata ttctgtagtg ccaaccagaa
    10801 aagccacaga agtatctgtt ttttcattta catgctcaat agaacgtaaa ttgcctagtt
    10861 ttagaagtat taaatcaaaa aacgaattgt gctcaccgac aatttgtaat ggcaaggttt
    10921 ctttgttcgg gatatttttt tgcacacgaa tagtacgaat gcctgcaatt atttctttta
    10981 ttttttcaaa acgtacaatt aattcttcat catattcgga tgctttcgat tgtaattgta
    11041 ccacaatact ttctccatct tgcctcgtgt atatcgattg ccacaattcc tcagtaataa
    11101 atggcataaa aggatgtaaa accaataata attcttcaaa aaattgcaaa gtagcttcgt
    11161 atgttacctt atctatcggt tgttgataag cgggcttcac catctctaag taccaggcag
    11221 agaattcatc ccaaaacaac ttatatacag ccattaatgc ttcagaaatc cgatacttgg
    11281 aaaataaatc atctacttct gttattgttt tagctaactg tgccttaaac catttaatag
    11341 caactccagc ggattctggc tgtggaatat ctgcaacctc ccaccctttt actaatcgaa
    11401 aagcattcca aattttatta ttaaagtttc ttccttgttc gcataacgat tcgtcaaaca
    11461 atatatcatt acctgcaggt gctgcaagca tcattcccat acgcacacca tcggcaccga
    11521 atcgttcaat aagctccaat ggatctggcg aattacctaa tgatttagac atttttctac
    11581 ctaacttatc acgaacaatc cctgtaaaat agacatgatt aaaaggctgt ttgttttcat
    11641 attcataacc tgccataatc atacgagcta cccaaaagaa aataatatca ggtcctgtaa
    11701 ctaaatcact agtaggataa taatatttta tttcttcatt attcggttct gtaataccat
    11761 taaacagaga tatgggccaa agccacgaag aaaaccacgt gtctaggcaa tcttcatctt
    11821 gacgtaaatc agcaagagtt aacgatggat tcattttttg ggctttttcc aaagcctctt
    11881 gttcggttaa tgctaccaca tatcctcctt caggtaagta atatgcaggt atgcggtgtc
    11941 cccaccaaag ttgacgactt atacaccaat ctttgatatt ttccatccag tggcgatacg
    12001 tatttttata tttggctgga taaaacttta cgtcatcagt cataacggca tctaatgcag
    12061 gcttggctat gtcttccatt ttcaaaaacc attgcatcga taatttgggc tcaataggaa
    12121 cattggttcg ttctgaataa cctaccttat tggtatatgg ttcaactttt tctaacaaac
    12181 cagctgcaag taaatcttgt tctatcttat ttcgcacatc aaacctatcc ataccagtat
    12241 actgacagcc atgttcattt aaggtgccat tgtcattaaa aatatctata cttggcaaat
    12301 tatatttttc acctagcatg tagtcgttta catcatgtgc tggcgttact ttcaaacaac
    12361 cagtaccaaa ttcagcgtct acgtattcat cttcaataac aggtatttct ctattaatca
    12421 aaggaacaat cacttttttc cctttcagga atgcattttt ggcatcattt ggatgaatac
    12481 acattgccgt atctcccata atggtttcgg gacgtgttgt agctactact gcataggcat
    12541 cttctcccac aactttatac tttaagtaat ataatttacc ttgttgttct ttaaaaataa
    12601 cctcttcatc agacaaggca gtaagagctt gtggatccca atttaccatg cgtactccac
    12661 gataaatttt ccctttttca aataaatcgc aaaaaacctt gagtacactt atcgaacgct
    12721 cctcatccat cgtaaaagcc gttctgctcc aatcgcacga agctcccaac ttacgtaatt
    12781 gatgt
//
LOCUS       DAOSRP010000052        12712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_4967_length_12712_cov_5.106107, whole genome shotgun sequence.
ACCESSION   DAOSRP010000052 DAOSRP010000000
VERSION     DAOSRP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4967_length_12712_cov_5.106107"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="tsaD"
                     /locus_tag="PLJ82_04555"
     CDS             complement(<1..582)
                     /gene="tsaD"
                     /locus_tag="PLJ82_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR40809.1"
                     /translation="MDNITILGIESSCDDTSAAVIRNGELLSNVIANQDVHIAYGGVV
                     PELASRAHQLNIVPTIHQAIKNASVSLSEIHAIAYTRGPGLMGSLLVGTSFAKGLALS
                     LGVPLIDVNHLKAHVLVHFIHDNTPNFKPPTFPFLCLLVSGGNSQIVLVKSYSEMEII
                     GQTIDDAAGEAFDKCAKILGGPYPGGPFVDVMAQ"
     gene            764..5167
                     /locus_tag="PLJ82_04560"
     CDS             764..5167
                     /locus_tag="PLJ82_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOR40810.1"
                     /translation="MLATLLLNTKPVQKYLLHQATHYLSNKIGSEVSISTVTYNLFTT
                     FEFSDIQIKDKERNDLLTIEHIDIKFKILPLFKQKLIVKSVSFIQPSINILRDSAGIS
                     NFQYILDAFAKNETDSIESKFVFDIRNITIKKGELKLTNIGTARKTQTFDPNHIHIWD
                     LNSEMRLHKIKSDSIVFELKSCNFEEQSGLIVKNLYADASFGKEYIHINDLSIKLPSS
                     IMEFEKLSIDYAHQENINSIVHMLDKSSIVLNSKKVVITGRDIACFVPRLAHLKNPIN
                     IEVNAQGANNTIELSKCIIKYGDQISLDASMQLAGINNMDETFIYADIHSISAHSTAI
                     ETVVSDIMRKPFILPKEIRNLGQMHYTGNIAGFFNNLVVYGKLRTDIGILSTDILLGI
                     DIQKNDIAYSGNLRSSRFQLTKMLGKDSGFGNIQFRVDVNGTKTHENKHNGIVKGNVQ
                     SIDFNKYTYKNIKLDGEYNNNGFNGKIELNDDNGQIKFDGDIDFSRKIPLMNFVAQVN
                     NVSPYKLQLTEHNPDLSLSFTIASSIQGTTIDNVIGNVFIQDINLHNASDTFEIKQVE
                     MKSFIEDEKQCLRITSDFLNATILGKITLSSIANNFLHIVHTEVPNISTLQRDKKLSG
                     NNFTFEANIEPIDQLSKVLLWDISTKEPTFIQGSFNDFTNIFSINAQSKGIQLNKNYL
                     YFATIDLHNSTSGIDLDANVGLKMPKDSLLVNVTSKILNNQGNTSISWKNSYGKEFNG
                     KLESVLDIKKNEDNEVETNIHILPTEMVVNDSIWHITNSTLVYKKERLLFDKFAITQH
                     DKFIRINGIASKLEDDWLYVDINQFNLEYLSELLQLKGIHLSGIATGSITAQQAFNKP
                     ILNASINATDFGLNSAPFGNVEVTANYNYEKEQIDIEGLITNHKLQQSHVSGYISPVR
                     NDMLLRADMHEVNVKFLDPYLSSFAKDIEGEATGVIYIGGDLRAIEVWGKAFINKAKL
                     HIDYLKTDVYFSDYITLTKNSILLSDITIADKKGNKGTVNGKITHKAFSEFLFNIDLL
                     LKNMLVYNTTENDNPDFYGTVYASGIANIYGNVDLLSIDVAGKTEKNTVFYIPINTST
                     SALEGDFITFVNRKQLSTTTRERRRTQQSESTESKLRISVQIEATPDAEAILILDDRT
                     REYIQGNGSGNIRLSLEPNENLKMYGNYTLNDGKYFFVMQKAIRKTFEIKDGSSIAWD
                     GNPYNGAIDIMATNQVSASLIDLLDASMLQDVKRLTVPVLCKVHLTGELQQPTIKLDL
                     ELPTADDELKRRVQNIVNSDEMMSNQMVFLLMLGRFYNPQLLTNTPGMATMIASATLS
                     SQLNYWLSQISNDVNLGVNYRQSEGGDITSREIEVALTTNLLDNRLIINSNVGYKEDA
                     FTETNFIGDFDIEYKINRSGRLRLKAYSHSNDKYYVRNALTTQGVGVIYREDFASGKT
                     LWNYYRRLFKLKAKNKTSQEGIVEEPNENIK"
     gene            5170..6081
                     /locus_tag="PLJ82_04565"
     CDS             5170..6081
                     /locus_tag="PLJ82_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR40811.1"
                     /translation="MITQTIGGNSHLQSSRIIMGCMRIDSLSQHDLQYLIDTAINNGI
                     SYFDHADIYANGYCEEHFGNSFRGNRENIVLQTKCGIKSGVFDFSKQHIISSVENSLS
                     RLKTSYIDILLLHRPDALVEPEEVAEAFETLFQQGKVLHFGVSNHNSHQLALLQKHIQ
                     HKLLINQLQFSIMHSGIIDAGFNVNMNTEQAIDKNGGVLDYCRLNDITIQAWSPFTYG
                     FFEGTFIDNERFWQLNQTLHTISTKYNVSKTAIATAWILRHPAKMQVVAGTTKPNRLQ
                     EICEGTTVTLTREEWYQIYLAAGNTLP"
     gene            complement(6078..7346)
                     /locus_tag="PLJ82_04570"
     CDS             complement(6078..7346)
                     /locus_tag="PLJ82_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOR40812.1"
                     /translation="MLPPLAERMRPQHLEEYIGQQHLVGEGAILRKMIESGSITSFIL
                     WGPPGVGKTTLARIIASKMNRGFYALSAVNSSVKDIREIIEKAKKTQFFDTPSPILFI
                     DEIHRFSKSQQDSLLAAVENGTVMLIGATTENPSFEVITPLLSRCQVYVLKHLEEQDL
                     RSLLNRVLEKDAVCKSKKIVVQETDALFRFAGGDARKLLNIIELLIGTENQTEIVLTN
                     KLVTEKLQENLAIYDKGGEMHYDIISAFIKSIRGSDPDAAIYWLARMIDGGEDPKFIA
                     RRLLISATEDIGLANPNALLLANTCFDAVHKIGFPESRIILAETTIYLASSKKSNSAY
                     LAIDKALDIVRKTGNLSVPLHLRNAPTSLMKNLEYGKNYVYPHDYPQNFTPQTYLPQE
                     LEKQSIWESQSNSTEKKLSEWLRSLWKNRF"
     gene            7532..9706
                     /locus_tag="PLJ82_04575"
     CDS             7532..9706
                     /locus_tag="PLJ82_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOR40813.1"
                     /translation="MNNEKATFLQAYQRLRRAVQPFAKKEDIHQIKQLYIHFFPSLGE
                     KEYRLMTKNFHVAAIAVEEIGLGRSAVMAILLYRLVVEEKITIQEIEKFYSKDIFIIV
                     KGLIQVNDLYTKTTSLETENFRRLLLTFAEDIRVIFIKLASRLYVMRELKSYEVDVQE
                     EIARETAFLYAPMAHRLGLYSIKTELEDLSLKHTQASIYKEIARKLNETKRSRDSYIE
                     QFIAPLKQKIIDAGFDFDIKGRTKSIYSIWNKIKKQDTPFENIYDLFAIRIILNSDIT
                     KEKADCWQVYSIVADMYQPNPKRLRDWLSIPKSNGYESLHTTVMGPQGKWVEVQIRTK
                     RMDEIAEKGLAAHWKYKGIQGESGMDEWLKNIREILENPELNAIDFMDEFKLNLYDKE
                     VFVFTPNGDLQKLPKGATLLDFAFHIHTGLGTKCVGGKANGKNVTLKYVLKNGDQIEI
                     QTSATQKPKLEWLNIVTTSKAKNKIRQAVKDEQFKDAQMGREILQRRFKNWKIEVEES
                     KISRLAKKKGFKTVSDFYQELAQEKLDILSLRDAYIDLDKRETTEPSDYRSAENFVQD
                     NTLQDTTSKEDVLIIDQNLRGIEYSLAKCCNPIFGDDVFGFITTSGGFKIHKKDCPNA
                     PQLISRFGYRIIPARWSGKSGTSQYAITLGVIGVDDIGIVTNISSLLSKEKNITLRSI
                     SVNSHDGLFEGRITIMVGELQELENIIKKIKTVKGVKSVQRI"
     gene            9760..10416
                     /locus_tag="PLJ82_04580"
     CDS             9760..10416
                     /locus_tag="PLJ82_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOR40814.1"
                     /translation="MLHTNPCNKTILFDFDGTLANTLKTVVEIYNKVAPEYNCKTVEL
                     EDVSRLQAMTISHLMKEQGISHITLAILLVRVRKELHINIDHVKPFFGIEEQLQTLKK
                     MGYQLGIMSSNSQKNVHTFLESNNMKHLFDFVHTSKNIFGKHTAIKRIISNLSLQTND
                     VVYVGDETRDIEACKKIGVKIAAVSWGYNLPEVLQAMQPDVLIDEPHSLANCISKLAM
                     "
     gene            complement(10421..11686)
                     /locus_tag="PLJ82_04585"
     CDS             complement(10421..11686)
                     /locus_tag="PLJ82_04585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR40815.1"
                     /translation="MTFEQLNLVEPILNALKHEGYTTPTPIQEVSIPIILKGTDLLGC
                     AQTGTGKTAAFAIPILQHLYLDKGSDRAPRKIKVLIVTPTRELAIQIGESFAAYGRLM
                     DVRHTVIFGGVNQQSQVNALKNGVDILVATPGRLLDLQTQGYIKLKDLTTFVLDEADR
                     MLDMGFIHDINKILKLIPAKRQTLFFSATMPNPIRVLANTMLTKPEEVSVTPVSTTAE
                     TIQQEVYFVDAANKRELLVHVLQDKKIDTALVFTRTKHGADKVQRMLSKAKIQTEAIH
                     GNKSQNARQNALRNFKDRTTRVLVATDIAARGIDIDALNLVINFEIPNIPETYVHRIG
                     RTGRAGLDGKAVSFCDVEEIPYLRDIHKLIGMQIPVVSDHPFPSSNVLTEVVDNKNRA
                     KKKPTIQPSKGKRRPFVSNKPTLVKQPRG"
     gene            11987..12586
                     /locus_tag="PLJ82_04590"
     CDS             11987..12586
                     /locus_tag="PLJ82_04590"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOR40816.1"
                     /translation="MFSGIIEEAAKIVQLDKDQENVHITVECSFVNELKIDQSVAHNG
                     VCLTVVKKTPTTYTVTAIKETLERSNLGQLAVGDKINLERSMLMNGRLDGHIVQGHVD
                     QTAICTQIEEADGSWYFTFSYQVSKEMAKKGYFTVDKGSVTVNGVSLTVCNPTDNTFQ
                     VAIIPYTYEHTNFHQLQVGSVVNIEFDIIGKYVSRMMGL"
BASE COUNT         4535 a         2175 c         2183 g         3819 t
ORIGIN      
        1 ctgagccatc acatcaacaa acggaccacc gggataaggt ccgccaagta ttttggcaca
       61 cttatcaaaa gcctctccag cagcatcatc aatggtttgt cctataattt ccatctcgct
      121 atatgatttc accagtacaa tttgtgaatt accccctgac accaacaaac ataaaaaagg
      181 gaaagttggt ggcttaaaat ttggagtatt atcatgaata aaatgaacca gcacatgtgc
      241 ttttaaatga tttacatcaa ttaaaggaac tcctaatgac aatgctaatc cttttgcaaa
      301 agaagtacct acgagtaaag agcccatgag ccctggtcca cgagtatagg ctatggcatg
      361 tatctcactt aacgaaaccg aggcattttt aatagcttga tgtatggttg gtacaatatt
      421 gagttgatgt gctcttgaag ctaattctgg aactacccca ccataagcaa tatgtacatc
      481 ttgattagca ataacattag acaacaattc cccgttacga ataacagcag cagaagtatc
      541 atcgcacgat gattcaattc caagtatagt aatattatcc ataaattaca gaaaaagaaa
      601 ctcaaattat ccttaaaaaa acaaaatatc tactgcttat tggttttcta ttttatttaa
      661 caggttttat tgttattttt gtaggttgaa attaaagtgc aaagttatta aaaaatatgt
      721 aactataaca agtattacgt tagcaattgt agcaggacta cttttgctag caactttatt
      781 actcaatacc aaaccagtac aaaaatattt gttgcatcaa gccacccatt acctttccaa
      841 caaaattgga agcgaagttt ctatttcaac tgtaacatat aatttattta cgacatttga
      901 attttcagat attcagataa aagataaaga acgtaatgac ctactaacta ttgaacatat
      961 agacattaag tttaaaatac ttcccttatt taaacaaaaa ttaattgtca aatcagtctc
     1021 tttcattcaa ccatctatca atatactaag agatagtgcg gggattagta actttcaata
     1081 catactggat gcttttgcta aaaatgaaac agacagtatt gagagtaagt ttgtattcga
     1141 tatcagaaat ataacaataa aaaagggaga actaaaacta acgaatatag gtaccgcacg
     1201 caaaacccaa acttttgatc ctaatcatat tcacatttgg gatttgaact cagaaatgcg
     1261 tttacacaaa attaaatcgg attctatcgt ttttgaattg aaatcatgta attttgaaga
     1321 acaatctgga ttaattgtaa aaaacttata tgctgatgct tcgtttggta aagaatatat
     1381 ccatatcaat gatttatcta ttaagctacc ttcttccatt atggaatttg aaaaactttc
     1441 tattgattat gctcatcaag aaaatatcaa ttctattgtc cacatgctag acaaatcttc
     1501 tattgtgctc aactcaaaga aggtagttat aacaggtaga gatattgcct gttttgttcc
     1561 tcgcttagca catttaaaaa atccgataaa catagaagta aatgcacaag gagctaacaa
     1621 caccatagaa ctgagcaaat gtattattaa atatggagat caaatatctt tggacgcatc
     1681 aatgcaattg gctggtatta ataacatgga tgaaacattt atatatgccg acattcattc
     1741 aatctctgca catagtacgg caatagaaac agtggtttca gatattatgc gaaaaccatt
     1801 tatactacca aaagaaatac ggaatttggg acagatgcat tatactggaa atattgctgg
     1861 attttttaac aatttggtgg tatatggaaa actgcgtaca gatataggca tattatccac
     1921 ggatatatta ttaggtattg atatacaaaa aaatgacatt gcatatagtg gaaatttgcg
     1981 ttcttctcgt tttcaactaa cgaaaatgtt aggtaaagat tctggttttg gaaatattca
     2041 atttagggta gatgtaaatg gcaccaaaac acatgaaaac aaacacaatg gtattgtaaa
     2101 agggaatgta caatcaatag atttcaacaa atatacgtat aaaaatatca aattagatgg
     2161 agaatacaat aacaatgggt ttaatggaaa aattgaatta aacgatgata acggacaaat
     2221 aaaatttgat ggtgacattg atttttctcg aaaaattcct ttgatgaatt ttgttgccca
     2281 agtgaataat gtgagtccgt acaaattgca acttactgaa cataatccag acttgagtct
     2341 ttcgtttaca attgcttcct ctatacaagg tacaactatt gataatgtta ttggtaatgt
     2401 atttatacag gacattaacc tccataatgc aagcgataca tttgaaataa aacaagtaga
     2461 aatgaagtcg ttcatcgagg atgaaaaaca atgtttgcga ataacatctg actttttgaa
     2521 tgcaacaatt cttggtaaaa ttacactatc gtctattgcg aataattttc tgcatatagt
     2581 acacaccgaa gtgccaaata taagcacctt acaacgggat aaaaaattaa gtggaaataa
     2641 ttttacattt gaggctaaca ttgagccaat agatcaatta tcaaaagtat tattatggga
     2701 tattagcaca aaagaaccaa cttttataca gggttctttt aatgatttta caaatatttt
     2761 ttctatcaac gcacaaagca aaggaataca attaaacaag aactatcttt attttgctac
     2821 gattgattta cataatagta cttcgggtat cgaccttgat gccaatgtag gattaaaaat
     2881 gccaaaagat agtttattag tcaatgtaac aagcaaaatt cttaataatc aaggaaatac
     2941 atctatttct tggaaaaata gctatggcaa agaatttaat ggtaaactag aaagtgtact
     3001 tgatatcaaa aaaaatgaag ataacgaggt agaaacgaat attcacattt tacctacaga
     3061 aatggtggta aatgattcaa tatggcatat tacaaacagt acattagtgt ataaaaaaga
     3121 acgtttattg tttgataaat ttgctattac acagcacgat aagtttattc gaataaatgg
     3181 aatagcatca aaattagaag atgattggtt gtatgtggat atcaaccaat ttaatctaga
     3241 atatttaagt gagctactcc aacttaaagg catacatctg tctggtattg caactggaag
     3301 cattactgca cagcaagctt tcaataaacc aatactaaat gcatctatca atgctacaga
     3361 ttttggttta aatagtgccc cttttggcaa tgtagaagta accgcaaact ataattacga
     3421 aaaagagcaa atagatattg aaggattgat aacaaaccac aaacttcaac aatctcatgt
     3481 aagtggatac atatctcctg tacgcaatga tatgctttta cgtgctgata tgcatgaagt
     3541 gaatgtgaaa tttttagatc cctacttatc ttcatttgcc aaagatattg aaggagaagc
     3601 tacgggtgta atatacattg gtggcgacct acgagcaata gaagtatggg gaaaagcttt
     3661 cataaataaa gctaaattac atattgatta tcttaaaaca gatgtatatt tttctgacta
     3721 tataacctta acaaaaaatt ctattttgtt atctgacata accattgcgg ataaaaaagg
     3781 gaacaaaggt accgttaacg gaaaaattac acataaagca ttttctgagt ttttatttaa
     3841 cattgattta ttgctcaaaa atatgttggt atacaacaca acagagaatg ataatcctga
     3901 tttttatgga acagtatacg catcaggtat agcaaatata tatgggaatg tggatttact
     3961 atcaattgat gtagcgggaa aaacagaaaa aaatacggtc ttttatattc caatcaacac
     4021 aagtactagt gcactagaag gagactttat aacctttgtc aacagaaaac aactttctac
     4081 aacaacaaga gagagaagac gtacccaaca atcagaatca actgaatcaa aactacgcat
     4141 atcagtacaa attgaagcca caccagatgc tgaagcaatt ctaattttag atgatagaac
     4201 acgagaatac attcaaggta atggcagtgg caacattcga ctaagcttag agccaaacga
     4261 aaacttaaaa atgtatggta attacaccct aaatgacgga aaatattttt ttgttatgca
     4321 aaaggctatc agaaaaacat ttgaaatcaa agacggaagt agtatagctt gggacggaaa
     4381 tccgtataat ggagccatcg acataatggc gaccaatcaa gtatctgcat ctcttattga
     4441 tttattagat gccagtatgt tgcaagatgt aaaaagactc accgttcctg ttttatgtaa
     4501 agttcattta acaggagaat tacagcaacc tactattaaa ttagatttag aactacctac
     4561 tgctgacgat gaattaaaac gaagagtgca gaatatagta aactctgacg aaatgatgag
     4621 caatcaaatg gtctttttat tgatgttagg gcgtttctat aatcctcagc tacttactaa
     4681 cacaccaggg atggcaacta tgatagcctc tgccacactt tcaagccaat tgaattattg
     4741 gttatctcaa attagtaacg acgtaaacct tggtgtaaat tatcgtcaaa gtgaaggagg
     4801 agacattact agtagagaaa tagaagtagc tttaacgact aatttacttg ataataggct
     4861 tataataaat agcaacgtgg gatacaaaga agacgccttt acagaaacta attttattgg
     4921 cgattttgac atcgaatata aaatcaatcg ttctggcagg ttacgcctca aagcttactc
     4981 tcactctaac gataaatatt atgtgcgtaa tgctcttact acacaaggtg ttggtgtcat
     5041 ataccgcgaa gacttcgctt cagggaaaac tctttggaat tactatcgaa gattatttaa
     5101 actcaaagcg aaaaacaaga ctagccaaga aggaatagtt gaagaaccta atgaaaatat
     5161 aaaataaaca tgataacaca aacaattggt ggtaattcac atctgcaatc atctcgtatt
     5221 attatgggtt gtatgcgtat cgactcctta tctcaacacg atttgcaata ccttattgat
     5281 acggctatta acaatggcat tagctatttc gatcatgctg atatatatgc caatggttat
     5341 tgcgaagaac attttggaaa tagttttcgt ggcaacagag aaaatattgt tttacaaaca
     5401 aaatgtggga taaaatcggg ggtattcgat ttttccaaac agcatattat tagcagtgta
     5461 gaaaacagtc tttctcgctt aaaaacctca tacatagaca tattgctact acaccgacca
     5521 gacgctctgg tagaacctga agaagtagct gaagcttttg aaaccttatt tcaacaaggc
     5581 aaagtgctcc attttggtgt tagtaaccac aactctcatc aacttgcatt attacaaaaa
     5641 cacattcaac acaaacttct tatcaatcaa ttgcaattta gtattatgca cagtgggata
     5701 atagatgctg gttttaatgt aaacatgaac acagagcaag ctatagataa aaatggagga
     5761 gtacttgatt attgtagatt gaatgatatt accatacaag catggtcgcc atttacgtat
     5821 ggtttttttg aaggaacatt tatagacaat gagcgttttt ggcaactcaa tcaaaccttg
     5881 cacacaatta gcaccaaata caatgtatct aaaactgcca ttgcaacagc atggatactc
     5941 cgacacccag ctaaaatgca agttgtggct ggcaccacca aaccaaatag gttacaagaa
     6001 atttgtgaag gtaccacggt aacacttacg cgtgaggaat ggtatcaaat ctatctagct
     6061 gctggaaata cattgcctta aaaacgattt ttccacaacg aacgcaacca ctccgacagt
     6121 tttttttccg tactattcga ttgactttcc catattgatt gcttttcaag ttcttgtggc
     6181 aagtacgttt gtggggtgaa attctgtgga taatcgtgcg gatacacgta atttttgcca
     6241 tactctaagt ttttcatcaa ggatgtagga gcattacgca aatgcaatgg aacggaaaga
     6301 tttcctgttt tacgaacaat atcaagggct ttatcaattg ccaaataggc tgaattgctt
     6361 tttttactac tcgccaaata tatcgttgtt tcagctaata taatacgtga ttcgggaaaa
     6421 ccgattttat gtacagcatc aaaacaagta tttgctaata acaatgcgtt cggattagct
     6481 aatccaatat cttctgtagc tgaaataagc aacctacgag ctataaactt ggggtcttct
     6541 cccccatcta tcatacgagc tagccaataa attgctgcat caggatcgct tcctctaatc
     6601 gatttaataa acgcagagat aatatcgtaa tgcatttcgc cccctttgtc gtaaatagcc
     6661 aagttttctt gtaatttttc ggttacgagt ttattcgtaa gaactatctc tgtttggttt
     6721 tctgtaccaa ttaagagttc gataatattg agcaacttac gtgcgtctcc acccgcaaaa
     6781 cggaacaaag catcggtttc ttgaacaaca attttcttgc ttttacacac tgcatctttt
     6841 tctaatactc gattgagcaa agagcgtaaa tcttgctctt ctaagtgttt taacacatat
     6901 acctgacacc tagataataa aggagtaatt acttcaaatg atggattttc tgttgtggct
     6961 ccgataagca taactgtacc attttctacg gcagccaata acgaatcctg ttgtgattta
     7021 ctaaaacgat gaatctcatc aataaaaaga ataggactag gtgtatcaaa aaattgcgtt
     7081 ttttttgctt tttcaattat ttctcgtata tcttttacac tcgaatttac agcactcaag
     7141 gcataaaaac ctctattcat ttttgaagca atgatacgtg ccaaggtcgt tttaccaaca
     7201 cctggaggac cccataaaat aaaagacgtt atactacccg attctatcat tttacgcaaa
     7261 atagcacctt caccaactaa atgttgttgt ccaatatatt cttctaaatg ttgcggacgc
     7321 atccgctctg ctaatggtgg taacatggct atgtgcttgt atgtatgatg acaaaggtac
     7381 atttattttt tagtttaata gacaaaaata aggtaaaata tgcttgtaca acaagtagat
     7441 ttttttgaat tacaagtaat aaaaaaataa aaaaagacac tagttgcact atattttttg
     7501 tatttttacg acattaacta aaagcaaaac aatgaacaac gaaaaagcta cctttttaca
     7561 agcctatcaa cgattacgca gggctgttca accttttgct aaaaaggaag atatacacca
     7621 aatcaagcaa ttatatatac acttttttcc ctccttagga gaaaaagaat accgcttaat
     7681 gacaaaaaat tttcatgttg cggctatcgc tgttgaagaa atcggtcttg gtcgttcggc
     7741 tgtaatggct attctactat accgattggt agtagaagaa aaaattacca tacaagaaat
     7801 tgaaaaattc tacagcaaag atatttttat tattgtgaaa ggattaattc aagttaacga
     7861 cttatacacg aaaacaactt cgctcgaaac cgaaaatttc agacgcttgc tactcacatt
     7921 tgccgaagat atacgggtaa ttttcattaa attggcaagt agattatatg tcatgcgtga
     7981 attaaaatcg tatgaagtag acgtgcaaga agaaattgct cgtgaaacag cttttttata
     8041 tgcaccaatg gctcatcgat taggtttata ctcaattaaa actgaattag aagatctatc
     8101 gctaaagcat acacaagcta gcatatacaa agaaattgct cgcaaactca atgaaacaaa
     8161 acgctcccga gatagctata ttgagcaatt cattgccccg ttgaaacaaa aaataatcga
     8221 tgcaggtttt gattttgata taaaaggaag aacaaaatct atttacagta tttggaataa
     8281 aataaaaaaa caagatacgc cttttgaaaa catttatgat ttgtttgcta ttcgtattat
     8341 tctcaacagc gatataacca aagaaaaagc cgattgttgg caagtatatt caatagtagc
     8401 agacatgtac caacccaatc caaaacgttt acgcgactgg ctatccatac ccaaaagcaa
     8461 tgggtacgaa tcgctacata ctacggtaat gggaccacaa ggcaaatggg tagaagtaca
     8521 aatacgtacc aaacgcatgg atgaaatagc tgaaaaaggt ttagcagcac actggaaata
     8581 caagggtata caaggagaat cggggatgga cgagtggctg aaaaacattc gtgaaatatt
     8641 ggaaaatcct gaattgaatg cgattgactt tatggatgag tttaagctca acttatacga
     8701 caaagaagta tttgtattta cacccaatgg cgatttgcaa aaactaccga agggagctac
     8761 tctacttgac tttgcctttc atattcatac ggggttggga acaaaatgtg tgggaggaaa
     8821 agcgaatgga aaaaatgtta ctttaaaata tgtactaaaa aatggagacc aaatagaaat
     8881 acaaacctca gctactcaaa aaccaaaatt agaatggttg aatatcgtaa ccacctctaa
     8941 ggcaaaaaat aaaatccgtc aagctgttaa agacgaacag tttaaagacg ctcagatggg
     9001 tagagaaatt ttgcaacgta ggtttaaaaa ctggaaaatt gaagtagaag aatcaaaaat
     9061 atcgcgtttg gctaagaaaa aagggtttaa aacagtaagt gatttttacc aagaattagc
     9121 acaagaaaaa ttggacatcc taagcctacg tgatgcttat atagatttag ataaaaggga
     9181 aacgacagaa ccttcagatt atcgaagtgc tgaaaatttt gtacaagata atacattgca
     9241 agatactact agtaaagaag atgtattgat aattgaccaa aacttgcgtg gaatcgaata
     9301 ttcattagca aaatgttgta acccaatttt tggcgatgac gtgtttggct ttattacgac
     9361 tagtggtggt tttaaaattc ataagaaaga ctgtccaaat gcacctcaac tcatttcacg
     9421 ttttggatac agaattatcc ctgctcgttg gtcaggaaaa tcggggactt cgcaatatgc
     9481 cattacactc ggagtaatcg gggttgacga tattggtatt gtaaccaaca taagttcgtt
     9541 gttatcaaaa gaaaaaaata ttaccttacg ttctatttcg gtaaattcgc atgatggact
     9601 ttttgaggga agaattacca ttatggtagg agagttacaa gaattagaga atattataaa
     9661 aaaaataaaa acagtaaaag gcgttaaaag tgttcaacgt atataaaaca ggcatttttt
     9721 attccctgac aattaatttt tatacaattt attcatatta tgcttcatac aaatccttgc
     9781 aataaaacca ttttgttcga ttttgacgga accttggcaa acaccctgaa aacagtagta
     9841 gaaatttata acaaagttgc tcccgaatac aattgtaaaa cggtagaatt agaagatgtg
     9901 tcgaggctac aagcgatgac aatatcgcat ttaatgaaag aacaaggcat ctcacatatc
     9961 actttagcta ttttgttggt acgagtacgc aaggaattac atatcaacat cgaccacgtg
    10021 aaaccattct ttggcatcga agaacaacta caaacattaa aaaaaatggg ttatcaatta
    10081 ggaattatgt cttcaaactc tcaaaaaaat gttcacactt ttttagaatc aaacaacatg
    10141 aaacacctat ttgatttcgt tcacacaagt aaaaatattt ttggcaaaca cactgctatt
    10201 aaacgtatta tatcaaactt atcgctacaa acaaatgatg tagtatatgt aggtgacgaa
    10261 actcgcgaca tagaagcttg caaaaaaatt ggtgtaaaaa tagcggcagt tagttggggc
    10321 tataatttac ccgaagtatt acaagctatg caacctgatg tacttattga cgaaccacat
    10381 agcctagcaa actgtatatc taaactcgct atgtaatgcc ttatcctctg ggctgtttaa
    10441 ccaaagtagg tttattgctt acaaatggac gccttttgcc ttttgaaggc tgtatggtag
    10501 gtttcttttt cgctcgattt ttgttatcaa caacttctgt taatacattg ctactaggaa
    10561 acggatgatc cgacacaacg ggtatttgca tgcctattaa tttatgaata tcacgcaagt
    10621 aaggaatttc ttccacatcg caaaaagaaa ccgctttgcc atctaaccct gccctacctg
    10681 tacgcccaat acgatgcaca taggtttcgg gtatatttgg aatttcaaaa ttgataacca
    10741 aattaagggc atcaatatca ataccacgtg ccgcaatatc agtagctact aatacacgcg
    10801 tagttctgtc tttaaaattt cgcaaggcat tctgacgtgc attttgcgac ttattgccat
    10861 gaatggcttc tgtttgtatt tttgctttcg atagcatacg ctgtacttta tctgcaccat
    10921 gcttggtacg cgtaaacaca agggctgtat caatcttttt atcttgcaat acatgtacta
    10981 ataattctct tttgttggcg gcatctacaa aatatacttc ttgctgaata gtttcggcag
    11041 tggtagatac aggagtaacc gatacctctt cgggcttagt taacatagta ttagccagta
    11101 cacgtatagg attaggcata gtagcagaaa aaaacaaggt ttggcgttta gcaggtatta
    11161 atttcagtat cttgttaata tcatgaataa aacccatatc caacatacgg tcagcctcat
    11221 cgagcacaaa tgtagttaaa tctttcaatt taatgtaacc ttgtgtttgc aaatcgagta
    11281 atctgccagg tgttgctacc aatatatcta ctccgttttt taaggcatta acctgcgatt
    11341 gttggttgac gccaccaaaa ataacggtat gacgcacatc cattaagcga ccataagctg
    11401 caaaactttc gccaatctga atggctaatt ctctggtggg tgttacaatc aacactttta
    11461 ttttgcgtgg tgctctatcc gagcctttat ctaagtataa atgttgtaaa atgggaatag
    11521 caaaagccgc tgtttttcct gtgcctgttt gtgcacagcc caacaaatca gtacctttta
    11581 gtataatggg gatagaaact tcttgaatgg gtgtgggcgt agtataacct tcgtgtttta
    11641 gagcatttaa aatcggctct acaagattta attgttcaaa tgtcataata aattgggaat
    11701 gtattcattg ttctatcggt gtgtaaaatt ataacctata tgaatacaac tggcgacaaa
    11761 gatagactaa ataactggtt aaactgtttt tttagcaaaa taatatcact tgaagtatgt
    11821 atcaataagc catgatgtat ataatgaaac actattttta ggtaaataat ggggtattct
    11881 gtaagcatac tacctgcttt cttgagatat aaatatttta ggtacttgaa ataatacagt
    11941 aaattatcta tctttgtaag cgaataaaca cattaaactc actactatgt tttcaggaat
    12001 aattgaagaa gccgccaaaa tagtacaact ggacaaagat caagaaaatg tacacattac
    12061 agtagaatgc tcgtttgtaa acgaacttaa aattgaccaa agtgtagctc ataacggcgt
    12121 ttgccttacg gtagtaaaaa aaacaccaac cacctatacc gttacagcca tcaaagaaac
    12181 gcttgaacgc agcaatttgg ggcagttagc agtgggcgac aaaattaatc tagaacgtag
    12241 tatgctgatg aacggacgtt tagacggaca catcgtgcaa ggacacgttg accaaaccgc
    12301 tatttgcacc caaatagaag aagccgatgg cagttggtat tttactttta gctaccaagt
    12361 aagcaaagaa atggcaaaaa aaggctattt tacagtagat aaaggctctg ttacagtaaa
    12421 tggtgtaagc cttacagtat gcaaccctac cgataatacc tttcaagtag ctattatccc
    12481 ctacacttac gaacacacga actttcacca actacaagta ggctcagtag taaacatcga
    12541 atttgatatt atcggcaaat acgttagtcg gatgatgggg ctgtaaaaga atatattttt
    12601 tacgtcattt attgttttcc tgtagctaaa actcgtagtt ttgttcaatc taaattatat
    12661 atcaataatt atttatcgtt tttcgataaa tttattttga tttataataa aa
//
LOCUS       DAOSRP010000053        12282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5168_length_12282_cov_5.623702, whole genome shotgun sequence.
ACCESSION   DAOSRP010000053 DAOSRP010000000
VERSION     DAOSRP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_12282_cov_5.623702"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLJ82_04595"
     CDS             <1..1085
                     /locus_tag="PLJ82_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR40817.1"
                     /translation="LWWFFIPYIGLIMAFVFAWNRSRNINLLPADTTLFVSIILSVRN
                     EEKAIQSLLYSMQNQTYKNFELRIVDDSSEDETFSLCSQLSSQFPFMSLQKSEYQGKK
                     KSLRQGIYSAHSELIVCIDADVHYPPEWLATLVSFYQTENADLLIAPVKMKVTNTFYS
                     KLQALEFLSLQASTGASALFKKAIMCNGANIAFKKDVYLAVQNELVDTEVSGDDMFLL
                     AAIKKMGGKVRYVYSPKAIVDIYPSPSLSDFLQQRMRWLSKSASYSDSDVIFVSFTVL
                     FAQIAIVIAGALAIFNMMFVPIFISLFFSKWVVDSWLLYKASDFLGQKRLVLYSLLLS
                     LVYPFYVISVACCSMICPIKWKNRNI"
     gene            1096..2082
                     /locus_tag="PLJ82_04600"
     CDS             1096..2082
                     /locus_tag="PLJ82_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOR40818.1"
                     /translation="MKSVVYLALFSLLLFVSCQSNEQSTSSQKQETQDLIQNPFYNAD
                     SAYVFVANQVAFGPREPNTDAHKKCGDYMVATLQRFGAEVTEQRVQLKAYNNTILDAR
                     NIIGSFNPEQKTRIFLCAHWDSRPYSDNDTDAANYHTPVQGANDGASGVGVLLELARL
                     MQQVPPTIGVDIILFDAEDYGRPSFDKSADIGDSFCLGSQYWSRNPHKEGYKPKYGIL
                     LDMVGGKNPTFLQEYFSMLYAPDVVKMVWKKAHQLGYQDYFISQPGNPVIDDHYYINK
                     IANIPCIDIIHYSNQTGFVDTWHTIYDTMENIDKTTLGVVGRVVTAVVYSEK"
     gene            2088..2663
                     /locus_tag="PLJ82_04605"
     CDS             2088..2663
                     /locus_tag="PLJ82_04605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOR40819.1"
                     /translation="MLLPYVHKDLIECGCDEAGRGCLAGAVYAAAVILPTDFCCPELN
                     DSKKLTEKQRYALRPYIEQHALAFAIGVVSESEIDEINILNASFLAMHRAIEGLPIQP
                     QHLLIDGNRFKNKTTIPHTCIVKGDGKYASIAAASILAKTYRDDYMSTLHISYPCYGW
                     LQNKAYPTKAHRNAIRENGVSPFHRKTFRLL"
     gene            complement(2669..3919)
                     /locus_tag="PLJ82_04610"
     CDS             complement(2669..3919)
                     /locus_tag="PLJ82_04610"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOR40820.1"
                     /translation="MAQHMEWGAKALQAARRLNLLTDATINQILVKVAEEAIANTSYI
                     LAENQKDLDAMDKNNPMYDRLLLSTDRIQGIAADIRNVASLPSPLGRILEERTLPNGM
                     FLTKKSVPFGVIGIIYEARPNVSFDVFSLCLKSGNVCVLKGGTDAYHSNLAIVSVIKN
                     VLKQFDIDENCIHLLPAGREATADLLNAVDYVDLIIPRGSQGLIDFVRNNAKVPVIET
                     GAGVCHTYFDEFGDFKKGQSIIYNAKTRRVSVCNALDCLIINEKRLADLASLCLPLAE
                     KEVVIEADIKAYTFLHGKYPTHLLTKATAESFGTEFQSYRMSIKTVSNMEQAIEHIAT
                     YSSKHSEAIISENEHAIHQFEQQVDAACVYSNASTAFTDGAQFGMGAEIGISTQKLHA
                     RGPMALPELTSYKWIVKGNGQTRS"
     gene            complement(3921..5003)
                     /gene="proB"
                     /locus_tag="PLJ82_04615"
     CDS             complement(3921..5003)
                     /gene="proB"
                     /locus_tag="PLJ82_04615"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOR40821.1"
                     /translation="MVLKFKKIAVKIGSNVLTQKDGTLDIPRIEKLVTQIAQLHKAGL
                     EIILISSGAVAAGKSQLKVSKKIDPISARQLFSAVGQVTLMNHYHRLFSEHGIFCGQV
                     LTTKENFGSRRHYLNQKNCMSEMLAHNVIPIVNENDTISVTELMFTDNDELSGLIATM
                     MGMEALIILSNIDGIYNGNPEDKNSEVITTVEANKNLSQYIQTNKSSFGRGGMLTKTT
                     IARKVADEGIHVFIANGKKDNILLDLVNKEKHVICTQFIANRKEVSSVKKWIAHSDGF
                     AKGEVHVNAGARKALTGNKATSVLFVGITEILGEFEKDDIVKIIDENGKQLGVGKTQY
                     DNKKATQLIGKKGQRPLVHYDYLYLD"
     gene            5084..5875
                     /locus_tag="PLJ82_04620"
     CDS             5084..5875
                     /locus_tag="PLJ82_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOR40822.1"
                     /translation="MKKYLLLGTVLLVLVGCSTVALTGRKQLMLVSDAEILEASNLHY
                     TEFMSTAKVSTNAAATKQIKQIGANIAKAVETYLKENGREADIASFAWDFTLVQDTTI
                     NAFCLPGGKVVFYEGILPICANETGLAVVMGHEIAHAVAKHGNERMSQQILAQTGLSV
                     AGVALSGKSEQAQQLMSSVYGLGVNYGVILPYSRKHEYEADRLGMIFMAMAGYDPAGA
                     VAFWERMTAGKESSVPEFLSTHPSDTKRIQAMQNVLPEAQGYYKK"
     gene            5933..6448
                     /locus_tag="PLJ82_04625"
     CDS             5933..6448
                     /locus_tag="PLJ82_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40823.1"
                     /translation="MNKEVLFSESQRFKQWWLWGIVIAINALLLYGVYVQIMLGQAFG
                     NNPMTNTQLIIVTLLTIMLAFLFLSFRLDTIIRKDGIYVRFFPFHIRFKYYDWQILSK
                     FYVRPYYPITEYGGWGLRLGLFGKGVAYTVSGNMGFQLEFTHNKKLLIGTNKPTELAK
                     VLSQLEQFKQE"
     gene            complement(6456..8792)
                     /gene="topA"
                     /locus_tag="PLJ82_04630"
     CDS             complement(6456..8792)
                     /gene="topA"
                     /locus_tag="PLJ82_04630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOR40824.1"
                     /translation="MGKNIVIVESPAKAKTIEKFLGKDYHVVSSFGHIRDLKKKDISI
                     DFNNNYAPIYEVPTDKKKLVSELKKKASEADMVWLASDEDREGEAIAWHLFEVLSLTE
                     DKTKRIVFHEITKDAIINAIQTPRKIDKALVDAQQARRVLDRIVGFELSPILWKKIKP
                     SLSAGRVQSVAVRLIVEREREITTFKSEANYRITAVFITKDTQGKTVELQAELPKRFK
                     TKEEANRFLEVCKDAQFNIEDISKKPSKKSPAAPFTTSTLQQEAARKLGYSVAQTMMV
                     AQKLYENGLITYMRTDSVNLSALAINTAKAEIIHFAGEQYVKTRQYATKSKGAQEAHE
                     AIRPTYMNQHTIEGNRQEKFLYELIWKRTIASQMADAEIEKTTATIGISTSEERFIAS
                     GEVLTFDGFLKVYMESNDDEDTTEDTKMLPPLHVHDTLQYKKILSTERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNREYVEKGDKEGEKRTYNLLTLEKGTITDIIK
                     EEIVGTEKSKLFPTDIGSVVTDFLLANFASVVDYNFTAKVEKEFDIVASGKLEWQKAI
                     DKFYKGFHTLVEDSLQNSTKKVGERLLGNDPTTGKPISVKIGRYGAIAQIGLTTEEEK
                     PQFAALKKEQSIETITLEEALELFKLPRTVGEFEEKEVTIAVGRFGPYVKHASAFYSI
                     PKEIDPLKITLSGAIDLIEKKREDDKNKVLKTFAEDASIQLLNGRYGAYIKQNKNNYK
                     IPKSKKAEELTYEDCIEIIKSEASDGDKPKKKKGFKKK"
     gene            9005..9430
                     /locus_tag="PLJ82_04635"
     CDS             9005..9430
                     /locus_tag="PLJ82_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOR40825.1"
                     /translation="MDKSIISLQQLIANSGMKVTPQRLAVFEAVMSMRTHPTADAIIA
                     RVQEQHPHIAVGTIYNVLDALVDKKLIVRVKTDKDSMRYDAVLDDHHHLYCSQTGEIV
                     DYYNQELSLILTNYFKERGIPNFEIQDIKLQINGIYKPQ"
     gene            9452..10165
                     /locus_tag="PLJ82_04640"
     CDS             9452..10165
                     /locus_tag="PLJ82_04640"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOR40826.1"
                     /translation="MENNQEVYAMPRIGEKAPVFKAVTTQGEINFPADYSGSWVILFS
                     HPADFTPVCTSEFMTFATMEKQFNQANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMK
                     DVEVTFPLIEDITMEVAKKYGMMMPGESNTKAVRAVFVIDPQGIIRTIIYYPLSLGRN
                     FDELYRVILALQTADEFKVATPADWRPGDDVIISPAGSCGTAKDRMDGKEDGLECKDW
                     FFCTKKLDKDLVLGKILKK"
     gene            10461..>12282
                     /locus_tag="PLJ82_04645"
     CDS             10461..>12282
                     /locus_tag="PLJ82_04645"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOR40827.1"
                     /translation="MKKQLSVLLFLLCVWSVVLAQHRKEFTIVQTSIPPKIDAVPTDT
                     VWDAAYSIRDFIQQSPTNGGVPSQHTEVRMLYDNEAIYVLAMLYDTKPDSIYAEIGDR
                     DSGDEANADLFSVEINPYNDGLNATEFMVSAAGVQMDSKNDLQTMNKSWDAVWKSEVK
                     KTDIGWIVEMKIPFSALRFPNTNTQVWEINFFRLIKRNGEGITWNFVDKNKNGWLNQA
                     GEMHGIRGINPPLRLSFMPYIATYYNDNKFLIKGGLDVKVGLNESFTLDMMLIPDFGQ
                     TRADDTYLNLSSVETKYDEKRQFFTEGTELFSKGDIFYSRRIGGQPRNYNKVYSYTKE
                     NEILQRNPSETNLYNATKISGHTSSGWGIGMLNAITKKQVAEIKDTLTDVTRYVTTQG
                     VSNYNVMVVNKVIGKESYISWVNTNLLIPEQNYVSNVSGFDFKYMLKDHAFTGDAFVS
                     YNKERELSPISNELGYRANVTISKLKGTFRYEYLATILSDSYNPTDIGYLENNNIITN
                     TLILYYLNYTPTKSVNEVTSQLAIYYDNLYHPLLYSRLELAWNLKLLFPSFNSIKFYI
                     SATPIEKYDHFEPRVVGWKAKEPTAGYVGAEYSSDYRKKLA"
BASE COUNT         3642 a         2103 c         2348 g         4189 t
ORIGIN      
        1 ggttatggtg gtttttcatc ccgtatatag gtctaatcat ggcatttgta tttgcttgga
       61 acagaagtcg taatatcaat cttttgccag cagatactac tctttttgta tctattatac
      121 tttcagtaag aaatgaagaa aaagcgatac agtcgttact ttatagtatg cagaatcaga
      181 catataaaaa ttttgaattg cgtattgttg atgattcttc tgaggatgaa acgttttctt
      241 tgtgttcaca attatcttcc caatttccat ttatgagctt gcaaaagagt gagtatcaag
      301 gcaaaaaaaa atctcttcga caagggatat atagtgctca cagtgaactt attgtatgta
      361 ttgatgctga cgtacactat cctcctgagt ggcttgctac gttggtttct ttttaccaaa
      421 cagaaaatgc tgatttactt atagctcctg taaaaatgaa agttacaaat actttttatt
      481 caaaattaca agccttagaa tttctaagtt tgcaggcttc aacaggtgcc agtgctttgt
      541 ttaagaaagc aattatgtgt aatggagcta atatagcttt taaaaaggat gtgtatcttg
      601 ctgtacaaaa tgagttggta gatacagaag tttcgggaga tgatatgttt ttgttagctg
      661 caattaaaaa aatgggaggc aaggtgcgtt atgtgtattc cccaaaggcg attgttgata
      721 tatatccttc tccatccctt tcggattttc tacaacaacg tatgcgttgg ctctcaaaat
      781 cagcatccta ttcagatagt gatgtcattt ttgtttcttt tacagtacta tttgctcaaa
      841 tagcaatcgt tattgctggt gctttagcta tttttaatat gatgtttgtg ccaattttta
      901 tatctttgtt ctttagtaaa tgggttgtag atagttggtt actctataag gcgtctgatt
      961 ttttaggaca aaaaagatta gttttatata gtttacttct ctcgttagtc tatccttttt
     1021 atgttatatc ggtggcttgt tgttctatga tttgccctat aaaatggaaa aatcgaaaca
     1081 tttaattata attctatgaa atcagtagta tacttagcat tattttcttt gctcttattt
     1141 gtatcatgtc aatcgaacga acagtctacc tcatcgcaaa agcaagagac acaagacctc
     1201 atccaaaatc cattttataa tgcggattct gcctatgtat ttgtagccaa ccaagtggca
     1261 tttggaccta gagaacccaa tacagatgca cataaaaagt gtggagatta tatggttgcc
     1321 actttacagc gttttggggc agaagtaaca gagcaaaggg tgcaattaaa agcatacaac
     1381 aatacaatac tagatgcacg taatattatt gggtcattta atccagaaca aaaaacacgt
     1441 atctttttat gtgcacattg ggattctcgt ccgtattctg ataatgatac agatgctgct
     1501 aactaccata cgcctgtgca aggagcaaat gatggagcga gtggtgtagg cgtattgctt
     1561 gaactagctc gtttaatgca acaagtacct ccaacaattg gtgtagatat tattcttttt
     1621 gatgcagaag attatggtag accctcattc gataagtctg ccgatatagg cgattcattc
     1681 tgtttgggct ctcaatattg gtcacgtaat ccgcataaag aaggctataa acctaaatat
     1741 ggtattttac tggatatggt agggggtaaa aatcctactt tcttgcagga atatttttcg
     1801 atgctctatg ctccagatgt ggtgaaaatg gtgtggaaaa aggctcatca attaggatat
     1861 caagattatt ttatttcgca gccaggtaat cctgttattg acgatcatta ttatattaac
     1921 aaaatagcca atattccctg tattgacatt attcattata gtaatcaaac gggttttgtt
     1981 gatacttggc atactatcta cgacaccatg gagaatattg ataaaacgac cttaggtgta
     2041 gttggtagag ttgtaacggc tgttgtttat tcagaaaaat aaggtgtatg ttgttgccat
     2101 atgtacataa agacttgata gaatgtggtt gcgatgaagc tggaagaggt tgtttggctg
     2161 gtgcagtata cgcagctgca gtaattttgc caaccgattt ttgttgccca gaactaaacg
     2221 actcgaaaaa actaaccgaa aaacaacgct acgccttacg tccatatatt gagcaacatg
     2281 ctttggcgtt tgccattggt gtggtttctg agagtgaaat tgatgaaata aatatcctca
     2341 atgcctcttt tttagccatg catcgtgcta ttgagggttt gcctattcaa cctcaacatt
     2401 tattaataga tggcaatcga tttaaaaata agaccacaat tccgcataca tgcatagtaa
     2461 aaggagatgg taaatatgca tccattgccg ctgcttctat tcttgccaaa acgtatagag
     2521 acgactatat gtccacattg catatttctt atccctgcta tggttggcta cagaataaag
     2581 cttatcctac caaggcacat cgcaatgcca tacgagaaaa cggtgtttct ccctttcacc
     2641 gaaaaacgtt tcggttactg taaagcgttt acgagcgagt ttgaccgttt cctttcacaa
     2701 tccatttgta gctagtaagt tctggtaaag ccataggacc tcgggcgtga agtttttgtg
     2761 tgctaatgcc aatttctgcc cccatcccaa attgtgctcc atcagtaaat gcggtagaag
     2821 cattgctata tacacaagca gcatctactt gttgttcaaa ttgatgtatg gcatgttcgt
     2881 tttcagaaat aatcgcttcg ctatgctttg agctataggt ggcaatgtgt tcaatagctt
     2941 gctccatatt gctcactgtt tttatactca tacgatagct ctgaaattcg gtgccaaaac
     3001 tttctgctgt ggctttagtt agtaaatgcg tagggtattt accatgtaag aaagtataag
     3061 cttttatatc agcctctatc actacttctt tttctgctaa tggaaggcaa aggcttgcca
     3121 agtctgctaa tcttttttca ttgattatca aacaatctag ggcattgcat acactaacgc
     3181 gacgagtttt tgcattataa ataattgatt gccctttttt aaagtcgcca aattcatcaa
     3241 aatacgtatg gcaaaccccg gctcctgttt ctattacagg tactttggca ttgtttcgca
     3301 caaaatcgat aagcccctga ctgccacgag ggataattaa gtctacataa tcaacagcat
     3361 tgagtaagtc tgccgtagct tccctaccag ctggcaatag atggatacaa ttttcatcta
     3421 tgtcaaactg ttttaataca ttttttatta ccgatacgat tgccaaatta gaatgataag
     3481 catctgtacc acctttaagc acacatacgt ttcctgattt taaacacaag gagaaaacat
     3541 cgaaactaac gttaggacgt gcctcataaa ttataccaat cactccgaaa ggaacacttt
     3601 ttttagtcaa aaacatacca tttggcaagg ttctttcttc cagtatgcga ccaagaggcg
     3661 agggcaatga tgccacattg cgtatatcag cagctatacc ttgtatccta tctgtactca
     3721 atagtaaacg gtcgtacatt ggattatttt tatccattgc atccaaatct ttttggtttt
     3781 cagccaaaat ataggaagta ttggcaatgg cttcttcagc cacttttact agtatctggt
     3841 ttatagttgc atctgtaagc aagtttaatc tacgtgctgc ttggagtgct ttagctcccc
     3901 attccatgtg ttgtgccata ttagtctaag tataaataat cataatgaac caaaggacgt
     3961 tgtccctttt tacctattaa ttgtgttgct tttttattat cgtattgtgt ttttcctacg
     4021 cctagttgtt tgccattttc gtcaatgatt tttacaatat catctttttc aaattcgcct
     4081 agtatctcgg taatgccaac aaacaatacc gaggtagcct tgttgcctgt tagtgctttt
     4141 cttgctcctg catttacatg tacttctcct ttagcaaatc cgtcgctatg agcaatccat
     4201 ttttttacac ttgatacttc tttgcgattg gcaataaatt gggtgcaaat tacgtgtttt
     4261 tctttattta ccaaatcgag cagtatattg tctttttttc cattggcaat aaatacgtgg
     4321 attccttcgt cagctacttt tcgtgcaata gtagttttgg taagcatacc gcctcgtcca
     4381 aacgacgatt tatttgtttg tatatattgt gaaaggtttt tgtttgcttc tactgtggta
     4441 attacttcag agtttttgtc ctcgggattt ccattataaa taccatctat attgctcaaa
     4501 attatcaatg cctccatccc catcattgtg gcaattagac cagagagttc gtcgttgtcg
     4561 gtaaacatga gttcggtaac cgaaatagta tcattctcgt tcacaatagg aattacattg
     4621 tgtgcaagca tttcgctcat gcagtttttt tgattgagat agtgtctgcg agatccaaaa
     4681 ttttcttttg tagtaagtac ttgtccacaa aagataccat gttcggaaaa tagacggtgg
     4741 taatgattca ttagagttac ctgaccgaca gctgaaaata attgcctagc cgaaatgggg
     4801 tctatctttt ttgaaacttt tagctgactt ttaccagcag ccacagcacc agaagagatg
     4861 agtataattt ctaatcctgc cttatgcaat tgggctattt gcgttaccag tttttcaata
     4921 cgaggaatat caagtgtccc atctttttga gttaggacgt tacttccaat ctttaccgct
     4981 atttttttaa attttaagac catttagggt agtttatgta ataaaatttt tacttttgca
     5041 aaagtaaaaa taaattgtta attagacgaa taaaaaaatg aatatgaaaa agtatttact
     5101 ccttggaaca gtcttacttg tattagttgg atgctcaaca gtagcgttaa caggcagaaa
     5161 acagctcatg ttggtatcgg atgcagagat attagaggca agtaatctac attacaccga
     5221 atttatgagt acagcaaaag tctctaccaa tgcagctgcc acaaagcaaa taaaacaaat
     5281 aggtgcaaat attgccaaag cagtagaaac gtatttaaag gaaaatggtc gtgaagctga
     5341 tattgctagt tttgcttggg attttaccct cgttcaggat accactataa atgctttttg
     5401 cttgccaggt gggaaagtcg ttttttatga agggatatta cctatttgtg caaacgaaac
     5461 cgggttagcc gtagtaatgg gacacgaaat agcacatgcc gttgcaaaac atggcaacga
     5521 acgtatgagc cagcaaatac ttgctcaaac cggattgagc gtagcaggag tggctttgtc
     5581 aggtaaatca gaacaagcac aacagcttat gagttcagtg tatggcttgg gagtaaatta
     5641 cggtgttatt ttgccttatt cgcgtaagca tgagtatgaa gcagatagat taggtatgat
     5701 ttttatggct atggcgggct acgacccagc aggtgcagta gctttttggg aacgtatgac
     5761 agcaggcaaa gaaagcagtg tgccagaatt tttgagcact cacccctctg atacaaaacg
     5821 aatacaagct atgcaaaatg tattaccaga ggctcaaggg tattataaaa aatgagttat
     5881 tacggtttac tagaaacgtt gaaaggctta atattccaaa aaacgatatt ccttgaacaa
     5941 agaagtctta ttttctgaaa gtcagcgatt taaacaatgg tggttatggg gtattgttat
     6001 agcgattaat gcacttttgt tgtatggtgt atatgtgcaa attatgcttg ggcaggcttt
     6061 cgggaataat ccaatgacga acactcaact tatcatagta acccttttga caattatgct
     6121 tgctttttta tttttgagct ttcgtctcga tacaattatt cgaaaagatg gtatttatgt
     6181 aaggtttttt ccttttcata tacgattcaa atactatgat tggcaaatac tgagcaagtt
     6241 ttatgtaaga ccctactatc caattactga atatggcggt tggggtttac gtcttggact
     6301 ctttggaaag ggcgttgctt atactgtatc tggtaatatg ggtttccaac ttgagtttac
     6361 acataataaa aaactcctta ttggaacaaa caaaccaaca gaattagcaa aagtactaag
     6421 tcaattagaa caattcaagc aagagtaatt ctttcttact tctttttaaa tccttttttc
     6481 tttttaggtt tatctccatc gcttgcttct gatttaataa tctctatgca atcttcgtat
     6541 gtgagctctt ctgctttttt cgactttggt attttgtaat tgtttttatt ttgttttatg
     6601 tacgcaccat aacgtccatt taatagttgt atagaggcat cttctgcaaa tgtttttagt
     6661 actttatttt tgtcgtcttc gcgtttcttt tctatcaaat cgattgctcc cgacagtgtt
     6721 atttttaaag ggtcaatttc tttggggata gaataaaatg cagaggcgtg ttttacgtaa
     6781 ggaccaaaac gcccaacggc tattgttact tctttttcct caaactcacc cacagttcgt
     6841 ggtagtttaa ataattctaa tgcctcttcg agtgtaatcg tctcgataga ttgttctttt
     6901 tttagtgcag caaattgtgg cttttcttct tcggtagtta acccaatttg ggctatagcc
     6961 ccatagcgtc caatttttac agatatcggt tttcctgttg ttgggtcgtt tcccaaaagt
     7021 ctttctccta cttttttagt ggaattttgc aacgaatctt caacaagagt atgaaaccct
     7081 ttgtaaaatt tgtcaattgc tttttgccat tccaatttcc ctgaggcaac tatatcaaat
     7141 tctttttcta ctttagctgt aaaattatag tctacaaccg aagcaaagtt ggctagtaaa
     7201 aaatctgtta cgaccgaacc tatatcggtt ggaaaaagtt ttgatttttc tgtaccaaca
     7261 atttcctctt ttatgatatc tgtaattgta cctttttcta atgtaagcaa attatacgtt
     7321 cttttttcac cttctttatc tcctttttct acgtattctc tattttgaat tgtactgata
     7381 gtaggtgcgt aggtagatgg tcgtccaatg cctaattctt ctaatttacg taccaaacta
     7441 gcctccgtgt atcgtggtgg tcgttgtgta aatcgttcgg tactaagtat ttttttgtac
     7501 tgcaaagtgt catgtacatg cagtggaggt agcatttttg tatcttctgt tgtatcttca
     7561 tcgtcattcg actccatata cacttttaag aaaccatcaa aagttagcac ctcgccacta
     7621 gctataaatc gttcttcgct agtagagata ccaatagttg cagtcgtttt ttctatttca
     7681 gcatcagcca tttgcgatgc aattgtacgt ttccaaatca actcatacaa gaatttttct
     7741 tgtcgatttc cttctatggt atgttgattc atgtaggtag gacgtatggc ttcgtgtgct
     7801 tcttgtgctc ctttcgattt ggtagcgtat tggcgagttt ttacgtattg ctctccagca
     7861 aaatgtataa tttccgcttt agctgtatta atagccaagg cagaaaggtt tacagaatcg
     7921 gtacgcatat acgtaataag tccattttcg tacaattttt gtgctaccat catggtttgt
     7981 gctaccgagt agccaagttt tcgggcagct tcttgctgca atgtagatgt cgtaaatggg
     8041 gcagcaggcg attttttgct cggttttttg cttatgtctt caatgttgaa ttgagcgtct
     8101 ttgcacactt ctaaaaagcg gtttgcttct tctttggttt taaaacgttt gggtaattcg
     8161 gcttgtaact ctaccgtttt tccttgtgta tctttggtaa taaacacagc agtaatgcgg
     8221 taatttgctt cagatttgaa agtagttatt tctctctctc gttctacgat taaacgaaca
     8281 gctactgatt gtacacgacc ggcactcaac gaaggtttaa tttttttcca caatatgggc
     8341 gaaagttcaa aacctacaat gcgatcgagc accctacgtg cttgttgtgc atctactagt
     8401 gctttgtcaa tttttctggg tgtttgtatg gcattgataa ttgcgtcttt ggtaatttcg
     8461 tgaaatacaa ttcgtttggt tttgtcttcg gtcaacgata atacttcaaa taaatgccaa
     8521 gcaatggctt ctccctcgcg gtcttcatcg gatgccaacc ataccatatc ggcttcagag
     8581 gctttctttt ttaattcgct tacaagtttc tttttgtcgg ttggtacttc gtaaatgggt
     8641 gcgtaattat tgttgaaatc tatgctaatg tcttttttct tcaaatcgcg tatatgacca
     8701 aaactagaca caacatggta atcttttcca agaaattttt caatcgtttt ggcttttgcc
     8761 ggagattcca caataacaat attctttccc atttttgtac caagttagca gattaattaa
     8821 caaaattcgg tgcaaaagta ataaaattga tattgataaa actatttttt tcgatataaa
     8881 tagttgtttt ttatggtttt tacagaaaag tgtgaaaatt aatgcaaaaa tatttggtta
     8941 attgaagaat ataaattatc tttgttctta aataggaatt attcctaata aataataatt
     9001 gtaagtggat aaatcaataa tttcattaca acaactcatc gcaaatagcg ggatgaaagt
     9061 aactccccag cgattagcgg tattcgaagc tgttatgtct atgaggactc accccacagc
     9121 tgatgccatt attgcacgtg ttcaagagca gcatcctcat atagcagtgg gcactatata
     9181 taatgtgtta gatgccttgg tagataaaaa attgattgta cgggtaaaaa ccgacaaaga
     9241 tagcatgcgg tatgatgctg ttttggacga tcatcatcac ttgtattgtt cgcaaacagg
     9301 agaaatagtt gattattata atcaagaatt aagtttgata cttacaaatt attttaaaga
     9361 aagagggata cctaattttg aaatacaaga tattaaatta caaataaacg gaatatataa
     9421 accacaataa taataaaaaa acaaaaagat tatggaaaac aatcaagaag tgtatgctat
     9481 gccacgtatt ggcgaaaaag ctcccgtttt taaggctgtt actacccaag gtgaaattaa
     9541 tttccctgct gattatagcg gtagttgggt aattttattt agtcatccag ccgattttac
     9601 tcctgtatgt acgtcagaat ttatgacatt tgctactatg gaaaaacagt ttaatcaggc
     9661 aaattgtaag ttggtaggac tttcagtcga tggtttgtat agtcatattg catggctacg
     9721 tactatcaaa gaaaaaattg agtacaaagg gatgaaagat gtagaggtaa cttttcctct
     9781 tattgaagat attacgatgg aagtagccaa aaaatatggt atgatgatgc ctggcgaaag
     9841 caatacaaaa gcggtaagag ctgtatttgt aattgaccca cagggaatta tccgaacaat
     9901 tatttattac ccattgagct taggacgcaa ctttgatgag ttgtatagag tgatattggc
     9961 attgcaaaca gctgatgagt ttaaggtagc gactcctgct gattggcgtc caggtgatga
    10021 tgtgattatt tcaccagcag gttcgtgtgg tactgccaaa gatagaatgg atggtaaaga
    10081 agatggactg gaatgtaaag actggttctt ctgtaccaaa aaacttgaca aagaccttgt
    10141 gttgggtaaa attcttaaaa aatagtattt caactttcct aaaaaggata gagctactca
    10201 gtatgagtgg ctcttttttt gcataaaaga gagttgtaag ctaaagtatg atcaaattat
    10261 acaatatgtt gcaacgatct tttatgttgg ttgcgtgtta tagttatcct tttttttcta
    10321 tattcttgtg tcagaagatt aacataaaga gtattttttt tctacgtttg cataaatagt
    10381 agtacgttgg tgtaatattt tttttgttgt cagtttattc tattttcttt ggaatgataa
    10441 ttatacctta tgttacgtct atgaaaaaac aacttagtgt gctactgttt ttattgtgtg
    10501 tatggtcagt tgtattggct caacacagaa aagagtttac cattgtgcaa acaagtatcc
    10561 cgcctaaaat tgatgctgtt cctaccgata cggtatggga tgcagcatat agtattcgcg
    10621 attttattca gcagagtcct actaacggtg gtgtgccttc gcaacacaca gaggtaagaa
    10681 tgctatatga taatgaggca atatatgtgt tggcaatgct gtatgacact aagcctgatt
    10741 ctatttatgc tgaaataggg gatagggatt cgggcgatga ggctaatgcc gaccttttct
    10801 cggtagagat aaacccctat aacgatggac tcaatgctac tgaatttatg gtttcggcgg
    10861 caggagtcca gatggattcg aaaaacgatt tacagaccat gaacaaaagt tgggatgctg
    10921 tgtggaaaag tgaagttaag aaaaccgaca tcggttggat agtggaaatg aaaattcctt
    10981 tttctgcatt acgttttcct aatacaaata cacaggtttg ggaaattaat ttttttcgtc
    11041 tgattaaacg taatggagag ggtataacat ggaattttgt cgataaaaat aaaaatggct
    11101 ggcttaatca agctggcgag atgcacggca ttcgtggtat taatcctcca ttacggcttt
    11161 cgtttatgcc ctatattgct acatactata atgataataa gtttttgata aaaggaggat
    11221 tggatgtaaa agtgggactc aatgaaagtt ttactctcga tatgatgctc attcccgatt
    11281 ttgggcaaac acgagccgat gatacgtatt taaatttgtc gtcagtggaa acaaagtatg
    11341 atgaaaaacg acaatttttt actgagggta ccgagctatt tagcaaaggc gatatatttt
    11401 attcacgtcg aataggaggt caacctcgca actataacaa ggtatattca tatacaaagg
    11461 aaaatgaaat actacaaaga aacccatctg aaacgaattt atataatgca acgaaaattt
    11521 ctggacatac ttcttctggt tggggaatag gtatgttaaa tgctattaca aaaaaacaag
    11581 tagctgaaat aaaagatact cttaccgatg ttacaagata tgtaactaca caaggggtat
    11641 ctaattacaa tgtgatggta gtaaataaag ttattgggaa agaatcgtat attagttggg
    11701 tcaatacaaa tttgctcatt cctgagcaaa attatgtgtc gaatgtgtcc ggttttgatt
    11761 ttaaatatat gttgaaagat catgcgttta ctggagatgc atttgtgagt tataataagg
    11821 aaagagagct ttctcctata tccaacgagc tgggttatag ggcaaatgtt actatctcaa
    11881 aactgaaagg gacctttaga tacgaatacc tcgcaactat tttatccgat tcatataatc
    11941 ctacggatat agggtatctt gaaaataata atatcattac gaatacgtta attttgtatt
    12001 atttaaatta tactcctacc aaatcagtaa atgaagttac tagtcaactg gctatttatt
    12061 acgataatct ttatcatcca ttattgtata gtagattaga gttggcatgg aatttaaaat
    12121 tgttatttcc atcatttaat tcaataaagt tttatattag tgcaacacct atcgaaaagt
    12181 acgaccattt tgaaccacgt gttgtaggtt ggaaagcaaa agaacctacc gctggctatg
    12241 tgggagcaga gtattcttct gattatcgta aaaaattagc tt
//
LOCUS       DAOSRP010000054        12226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5202_length_12226_cov_4.453373, whole genome shotgun sequence.
ACCESSION   DAOSRP010000054 DAOSRP010000000
VERSION     DAOSRP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_12226_cov_4.453373"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLJ82_04650"
     CDS             complement(<1..1012)
                     /locus_tag="PLJ82_04650"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOR40828.1"
                     /translation="MKKLYIYLLSSLIGCFAVVALYAQPNTMYFMNYLPYQSYMNPAI
                     QPACRVYVELPAISAINFSLGNNSLSMNDFVYMNQGQMVTALHPELGDKTSLLNAFSA
                     NTKFYQDLSFSLLGFGFKIKENGYLSINASLRQDMAFYIPKDFVKLALYGTPEENQVN
                     TFNLQSLGFDASAYMDLSAGYSHKINEQFTVGLRAKLLVGLLDANLGFNELELQASQE
                     EWRIIGNASARFSAPAVTVLVDNDGMIEEVAIPDDVLNSLTDYRPNLGLGFDLGAVYK
                     PIENLSLSVAIRDLGFISWKNGYQQSASIDFAFEGIEFDITQMDSDYADSLLTVLQDS
                     YTV"
     gene            complement(1088..2815)
                     /locus_tag="PLJ82_04655"
     CDS             complement(1088..2815)
                     /locus_tag="PLJ82_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40829.1"
                     /translation="MKNRILHQLLFLVFSLFFLLTSCIDDSYKIDDIDTTVGLDMALV
                     GPIGKSKITLSSVFPDDLENYELLIRNDSIFIVLHDTVDFGSTFIDDIKIPKGQYTKH
                     IPVIDSDLLPTTSVDVDVTLDYEFDINTNPNERIDSLVFNTSVLNFQFTSNYDYSPAA
                     QMEVIFDSDELVLNPTLYPENKIVLSLVQGTINQNIDLTGAKLFFPNSSKKIKIRYKA
                     HVETNTPFVPGAEPIDVAIDFDTTIPRIVYGYIGQNRDIWGGEIVRPFDFTSLFQGVD
                     FFLPFYNPQIYITAESNIGIPAQYTLDWVKATDTNTSEVVYADFEGSPNTSFVLNYPV
                     ISEIENKSIQELLSFDSRTLDKRHTKLFDREYGATNRLFQINANELAYKFSVRTVNDP
                     AASVHYFLNDSKMSLYIKAMIQLNFEGNDNPAKSFHVNLSDTLDIDFAQIIDVDQVDT
                     ENEIVARIKLNYKNALPIGAFASLSFLDENKEKVQYNNETLEKEFTIEPAQVKPDGTV
                     LAPLPNTSITINLTADEYTNYISKTKKLAFTYSMKGNDGKNVQIKASDWLELKASFYV
                     SAKAKVTPE"
     gene            complement(2897..4294)
                     /locus_tag="PLJ82_04660"
     CDS             complement(2897..4294)
                     /locus_tag="PLJ82_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR40830.1"
                     /translation="MLTRYIENKTTGYLGFTPTPEQAELISQLAVFLAEPQKRHTFLL
                     KGYAGTGKTSVVSAIVKLLDELQQPVILLAPTGRAAKVLSSYSGKQAATIHKHIYRKK
                     TAIADAPFDIAYNSYKNALFIVDEASMVANTGDGLFGSGRLLDDLITYVFSAENCHLL
                     LLGDTAQLPPVMQEESEALDTKILEKYDLYVQEYTLTQVVRQAEASGILRNATHIRQM
                     ISNQINIHSFILHTDSDVIRVSGQDFVETLYTAYRDGVDETILITRSNKKAAMYVEGI
                     RARILQREELLSAGDLVMVVKNNYFVGKEYQGFEFIANGDIAEVLRVRSYSQLYGFTF
                     ADVVVRFLDYDWEVDAKILVDVLSCVTPAELQEMNKKLFKTVEEDYAHIGNKRTRYKE
                     MKNDIYLNALQLKFAYAITCHKAQGGQWKHVFVDMGMIKKTEIDTSFLRWLYTASTRA
                     TQKLYLINFDDAFFC"
     gene            4474..5109
                     /locus_tag="PLJ82_04665"
     CDS             4474..5109
                     /locus_tag="PLJ82_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40831.1"
                     /translation="MDKIFKLILVAVSIVLGYLCVQSILIPIKFNEEKSFREKVVIAR
                     LADIRRAQVEYRTAKGVYTNSFDTLIAFVRQGKAPIILKEGSLTDKQLEAGLTEKEAV
                     RQGIIKRDTTYVSILESLFGKNYPIDSIRYVPFSNPLTEFEMEKGELTSGAAAISVKV
                     VEVRTPYTVYLNGLNRQEIINIVETQEKMNKYPGLKFGSVTEANNNAGNWE"
     gene            5118..5966
                     /locus_tag="PLJ82_04670"
     CDS             5118..5966
                     /locus_tag="PLJ82_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOR40832.1"
                     /translation="MMHLLVKDTAFDSKNTREYDLSIRISADGFSFCIYHPLTKTFVC
                     CKHVDFSVKDTKYYRNQAIALCEPYLAYEYRRTLVVFADEKSTLIPEGVFQPADYLNI
                     LHFNYPETKHSGYELLINNLTVFSITNIFAVYTEQITFWKEVFPSAEFIHMHTPLIDA
                     LLKESKKEDIQLVWLYIQPTSIFLSYADKGKLIFSNTFPIQSATDIVYACGNVFEQFG
                     LSQHHTKLILTGNYTSKSEIYSLLHTHFAMLDEYSLASDIIIQSSCKSFDIQKLMDLL
                     LVPLCV"
     gene            5957..6499
                     /gene="rsmD"
                     /locus_tag="PLJ82_04675"
     CDS             5957..6499
                     /gene="rsmD"
                     /locus_tag="PLJ82_04675"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOR40833.1"
                     /translation="MRIISGIYKGRRFTPPASLKARPTTDFAKEGLFNVLANEIDIEE
                     AQVLDLFAGSGSISFEFVSRGAGHTTSIEQSFQHGAFIKKTAQELNIKTLSVLKADVF
                     RYIETCNHSYDIIFADPPYSLPQLAEIPNKIMNSALLKPDGLFILEHGKNNTFGEHPN
                     FINTRHYGNVHFSFFKHKTK"
     gene            complement(6509..7552)
                     /locus_tag="PLJ82_04680"
     CDS             complement(6509..7552)
                     /locus_tag="PLJ82_04680"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR40834.1"
                     /translation="MKKSIFLYFLLSMYWLTQWLIVPCYAEIPFRVMCYNVENAFDCE
                     NDSLTNDDDFTPQGAYHWTPKKFKLKISNIAKVITAVGQFTPPSIVGLCEIENESVLK
                     SLVYFSPLRNLNYKYVHYDSPDKRGIDVALLYRPEQFRVLSSAPIGVSFASAPFSTTR
                     DILYVKGIAMYTDTLHIFVCHFPSRLGGELESEDRRIEVSRILRKQVDSLFLASPTST
                     MLLMGDFNDYPTNTSLFSILQAQDPQNAIIRSYGLYNLYYQYHNKPNIGTHKHATEWG
                     VLDQMIVSGNALQPDAFFYTSEQSAKIFDAEFLLIDDETYFGKKTFRTYLGMKYIGGF
                     ADHLPIYIDFFQR"
     gene            complement(7555..8952)
                     /locus_tag="PLJ82_04685"
     CDS             complement(7555..8952)
                     /locus_tag="PLJ82_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40835.1"
                     /translation="MVWIGFSSCEDMMFSTNPNQRIHISADTVSFDTLFTGVGSTTKI
                     FNIYNPSKNRIKIDAIELVGATHYSLNINGVLTDALHDISLKANDSMRIFVQAYVDPS
                     DQFTPFIVKDSIIIRSNTHTYKVVLQAYGQDAYRVSKRQIMADTTWLAEKPYLILDTL
                     TIAQGATLTLSEGVSLYFVKHASLQVYGTLKAKGIQSKPVVFRGDRMDNMFDNLPYDK
                     VPNQWQGIRFRKGSAHNELNYVVVKNTNQGIVLDSTSLDVLALAISNSIINNSATNLI
                     TASHTVMHISNSVVYNAGQSCLVLQGGKYNVVHSTIANYFSFPWVGRKATSVLLSNYI
                     NLGSQAMPVPLLEANFINSIVYGSWSNEVSVSSSYLNQKIEEPLVYVFKNSVIKISPA
                     TINDTNFLEIIANQDPKFNATKPYNYDFTLQSSSPALGNADNLVAAEYPIDMLGRQRL
                     FNDKADIGAYVIIAE"
     gene            9109..9396
                     /locus_tag="PLJ82_04690"
     CDS             9109..9396
                     /locus_tag="PLJ82_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40836.1"
                     /translation="MANKRNLKKDIQSIFSELLVECYTLSYIFPQIDIAKVDAIATSI
                     IEKSDNFVKRAGKPDGKDNPAIVKAYYKKLYKDVANTVSVFIEDINTLKSK"
     gene            9482..10045
                     /locus_tag="PLJ82_04695"
     CDS             9482..10045
                     /locus_tag="PLJ82_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR40837.1"
                     /translation="MKKNISKLLLKLMGWKTTASIPSIKKLVICVAPHTSNWDFIIGK
                     LFYTALGGKASFLIKQEWLKPPVGKLLINMGAIGVNRGKNTSLTDQLAKVFQEKEEFH
                     LAITPEGTRKKVDKWKLGFYQIAKKAGVPIAMITIDYKHKEVSVLGIYEPTDDEEKDI
                     NMIKSFYENAHAKYPSQFSLGKDYKQV"
     gene            10042..10827
                     /locus_tag="PLJ82_04700"
     CDS             10042..10827
                     /locus_tag="PLJ82_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOR40838.1"
                     /translation="MIQDNLTVSIIQTDIKWENKAKNLEMFAEKVRACGSYSDIILLP
                     EMFTTGFSMHASNLAETVHDETINTLKKWALTENAMLVGSFIAIDNNHVYNRGFAVLP
                     SQEIYWYDKKHLFSMSKEDAVFTPGNAPCIVPYKGWNISLAICYDLRFPVWLRNTNNA
                     YDMLLVVANWPESRKEAFNTLLKARAIENSCYVCAANRIGIDGNQVVYAGNSQIVDFK
                     GTIVANTTEHTDNIVSATVNKDLLIAFREKFPVWKDADPFSLL"
     gene            complement(10839..11354)
                     /locus_tag="PLJ82_04705"
     CDS             complement(10839..11354)
                     /locus_tag="PLJ82_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR40839.1"
                     /translation="MLHNTIITLRAIEPEDLDLFYKWENDTMYWEHGSTIVPFSRYAL
                     KQYIQATTQDVYETKQLRFMIEHVRDKVCLGTIDVSQIDFFHKRAEMGVFIDKHYQQK
                     GFAVMAIQLLLEYLFSFLDLEQVYCHVPKNNISSQKMLLKTGFVCVGEKKHWIRKGNE
                     FEDVLFFQKLK"
     gene            complement(11366..>12226)
                     /locus_tag="PLJ82_04710"
     CDS             complement(11366..>12226)
                     /locus_tag="PLJ82_04710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR40840.1"
                     /translation="QSRDLVSTYFSSVIYIANAENVGFSKANNQAIKIAKGEYVLLLN
                     PDTIIAEDTLKTVCDIADTTDRFGALGVRMVDMKGAFLPESKRGIPTVWVAFCKLTFI
                     HKLFPRSTFFNAYYYPNIKENELGEVPILAGAFMLINKKRLQEEALLDEQFFMYGEDI
                     DLSYRIINQGMKNVYVPTTIVHYKGESTKKGDMKYLKAFYESMLLFYKKHYAGSFPLW
                     FYPISGLIHVYVSIAYRCRIKRTRNRRAKETIVLSTNQYSYAEIIQQIEKNKGEKNIQ
                     LYHPNFGFSV"
BASE COUNT         4211 a         2226 c         1912 g         3877 t
ORIGIN      
        1 ctaccgtata tgaatcttgc aacacagtaa gcaaagaatc tgcataatct gaatccattt
       61 gcgtaatatc aaattctatt ccttcaaaag caaaatcaat cgaagcagat tgttggtaac
      121 catttttcca cgaaataaag cctaaatcac gaattgcaac cgataaactt aggttttcta
      181 ttggtttgta tactgcccca agatcgaaac caagccctag gtttggacga taatctgtca
      241 acgaattcaa aacatcatcc ggtattgcca cctcctctat cataccatca ttatcgacta
      301 atacagtaac tgcaggtgcc gaaaaacgag ctgaagcgtt tcctataatt cgccattctt
      361 cttgtgatgc ttgtaattct aattcattaa aacctaagtt agcgtcaagt aaccctacca
      421 acaatttggc acgtaatccg acagtaaatt gttcattaat cttatgcgaa taccctgcag
      481 acaaatccat ataagcactt gcatcaaacc ctaaactttg taaattaaat gtattgactt
      541 ggttttcttc tggagtacca tataaagcta attttacaaa atcttttggt atataaaaag
      601 ccatgtcttg gcgtaatgaa gcatttattg aaagatatcc gttttctttt attttaaagc
      661 caaaacctaa caaactaaaa gacaaatctt gatagaattt agtgtttgca ctaaaagcat
      721 taagcaagct cgttttatcg ccaagttctg gatgcaaagc tgttaccatt tgtccttgat
      781 tcatatacac aaagtcattc attgacaatg aattatttcc tagcgaaaaa ttaattgcag
      841 aaatagcagg aagttctaca tataccctac aagcaggctg aatagctgga ttcatataac
      901 tttgataggg taaataattc ataaaataca tggtgttagg ttgagcataa agagcaacaa
      961 cagcaaaaca gccaattaac gagcttagta aatatatgta tagttttttc atatttgctt
     1021 atttaatatt ttgtgtaacc aatcaatctg tttatgcgaa tgaataatta gattttttac
     1081 taagaactta ttctggcgtt actttcgctt ttgcagacac ataaaaactc gctttcaatt
     1141 ctaaccaatc acttgctttg atttgtacgt tctttccatc atttcctttc atagaatatg
     1201 taaaggcaag tttttttgtt tttgaaatat agtttgtata ttcatctgct gtcaaattta
     1261 ttgttatact tgtatttggc aaaggagcta ggacagtacc atcgggctta acctgagcag
     1321 gttcaatggt aaattctttt tctagtgttt cattgttata ttgaactttc tccttatttt
     1381 cgtccaaaaa tgaaagcgat gcaaatgctc caataggcag tgcgttttta taattcaatt
     1441 taatccttgc aacaatttca ttttctgtgt ctacttggtc tacatctatg atttgagcaa
     1501 aatcgatatc taaggtatcg gacaaattaa catgaaaact tttggctgga ttatcatttc
     1561 cttcaaaatt tagttggatc atggctttaa tatataaaga cattttacta tcattcaaaa
     1621 aataatgaac agatgccgct ggatcattta cagttcttac cgaaaatttg taagccaatt
     1681 cgttcgcatt tatttgaaat aaacgattag ttgcaccata ttctctatca aagagtttcg
     1741 tatgcctttt gtctaatgtt ctactatcaa aacttagcaa ttcttgtatt gatttatttt
     1801 caatttcact aatgactgga taattaagca caaaactcgt attgggagat ccttcaaaat
     1861 cagcatatac cacctcactc gtatttgtat cagttgcctt aacccaatct agagtatact
     1921 gtgcaggtat accaatatta ctttctgctg ttatataaat ttgcggattg taaaatggca
     1981 aaaagaaatc aaccccctga aacaatgaag taaaatcaaa cggtctaact atttctcctc
     2041 cccaaatatc tctattttga ccaatatatc cataaacaat acgaggtatc gtagtatcaa
     2101 aatcaatggc tacatcaatg ggttctgcac ctggcacaaa aggtgtattt gtttcaacat
     2161 gtgccttgta acgaatttta atttttttcg acgaatttgg gaaaaacagc tttgctccag
     2221 ttaagtcaat attttgattt attgtgcctt gtaccagaga taacacaatt ttattttcag
     2281 gatagagagt aggattaaga acaagttcat cagaatcaaa aataacctcc atctgagcag
     2341 cgggagaata atcgtagtta gatgtaaact gaaaatttaa taccgacgta ttgaaaacaa
     2401 gactatctat ccgctcattc ggattggtat tgatatcaaa ttcatagtcc aacgttacat
     2461 caacatcgac actcgtggta ggcaacaaat cgctatcaat cacaggtata tgctttgtat
     2521 actgcccttt aggtatttta atatcatcaa taaaagtact tccaaaatca acagtatcgt
     2581 gtaatacaat aaaaatactg tcgttacgaa tcaataattc gtaattttca agatcatcag
     2641 gaaaaacaga cgaaagcgta attttagatt tacctatagg accaactaac gccatatcca
     2701 gtcctaccgt agtatctata tcatcaattt tataactatc atctatacag cttgttaata
     2761 aaaaaaataa agaaaaaact aggaataaaa gttggtgtag tattctattt ttcatacgca
     2821 taaatttaat ggctcatttt tctttctaaa ttacttgtac gaagatagta caaaatttga
     2881 taaatcgaaa caatttttaa caaaaaaacg catcatcaaa atttattaaa tacaattttt
     2941 gggtagctcg agtactagca gtatacaacc aacgtaaaaa tgaggtatca atttctgttt
     3001 ttttaatcat tcccatatcc acaaaaacat gtttccattg tcctccttgt gctttatgac
     3061 aagtaattgc ataagcaaac tttagctgaa gtgcattcaa ataaatatca tttttcattt
     3121 ctttatacct agttctttta ttgccaatat gtgcataatc ctcctcaact gttttaaata
     3181 attttttatt catttcctgt aattcggcag gagtaacgca agacaagaca tctaccaaaa
     3241 ttttggcatc cacctcccaa tcgtagtcta aaaaccgaac aactacatca gcaaaagtaa
     3301 atccgtataa ttgtgaatag gaacgtacac gcaaaacctc tgctatatct ccgtttgcaa
     3361 taaattcaaa cccctgatac tccttgccaa caaaataatt attttttacc accattacca
     3421 aatctcctgc agataacaac tcttctcttt gtaatatcct agcacgaatt ccctccacat
     3481 acatagccgc ttttttgtta gaacgtgtaa taagaatggt ttcgtctact ccatcacgat
     3541 atgctgtata caaagtttct acaaaatctt gacctgacac acgtatcaca tcactatctg
     3601 tgtgtaaaat aaaactgtgt atatttatct gattacttat catttgtcgg atatgtgtgg
     3661 cgttgcgtaa aattccactt gcttctgcct gacgtaccac ttgtgtaagg gtatattctt
     3721 gcacatataa atcatatttt tcaaggattt tagtgtccaa tgcctcactt tcttcttgca
     3781 ttacgggtgg taactgggca gtatcaccca acaataataa atggcaattc tcagcactaa
     3841 acacgtaggt aatcaaatca tccagtaaac gaccacttcc aaacagacca tctcctgtat
     3901 ttgccaccat agatgcttca tctacaatga ataatgcatt tttataacta ttatatgcta
     3961 tatcaaaagg agcatcagct atggctgttt ttttgcgata aatatgttta tgaatggttg
     4021 ctgcttgttt gccagaatac gaagacaaga ctttggcggc tcttcctgtt ggtgccaata
     4081 aaataacggg ctgttgcaat tcatctaaca atttaacaat agcacttacc acagatgttt
     4141 tgcccgttcc tgcatatcct ttaagtaaaa aggtatggcg tttttgcggt tcagccaaaa
     4201 aaacagcaag ttgggatatc aactctgctt gttctggcgt aggagtaaat cctaaatagc
     4261 ctgtggtttt attttctata tatcgtgtaa gcattatcag acttatatta acgagtaaaa
     4321 ataagatata cgtgaaatat ttagtttgac acaaaagtaa tgaattaaaa aaaaattagc
     4381 tctacacatc tatttttttt tcaaataaag ataattagtt ccaaattctt tcttaacttt
     4441 gcgtcattct aaaaacaaaa ccttgtaaca ccaatggaca aaatttttaa actaatttta
     4501 gttgctgtaa gtattgtatt aggctattta tgcgtacaaa gtattttaat tcctatcaaa
     4561 tttaacgaag aaaaatcttt tcgtgaaaag gtagttatag cacgtttagc agatatcaga
     4621 agagctcaag ttgaatatcg cacagcgaaa ggcgtatata caaatagttt cgatacactt
     4681 attgcttttg ttagacaagg gaaagctcct attattctta aagaaggaag tttaaccgac
     4741 aaacaattgg aagcaggtct gactgaaaaa gaagctgttc gtcaaggaat catcaaaaga
     4801 gatacaacat atgtaagtat tttagaatcg ttatttggca aaaattatcc cattgattct
     4861 attcgttatg tcccattttc taacccttta actgagtttg aaatggaaaa aggggaatta
     4921 acttctggag cagccgctat aagtgtaaaa gtagtagagg taagaactcc atacactgtt
     4981 tatttaaacg gattaaatag acaagaaatc ataaatattg tggaaacgca agaaaaaatg
     5041 aataaatatc ctggattaaa atttgggtca gtaacagaag ccaacaataa tgcaggcaac
     5101 tgggaataga aacataaatg atgcacttat tggttaaaga tactgctttt gactctaaaa
     5161 atacccgtga atatgactta tccattcgca tatcagcgga tggattttca ttttgcattt
     5221 atcatcccct aacgaaaact tttgtatgtt gtaagcatgt agatttttcg gtgaaagaca
     5281 caaaatatta ccgcaatcaa gctattgctt tgtgcgaacc atatttagcg tacgaatacc
     5341 gtcgtacctt ggttgtattt gctgacgaaa aaagcacact tataccagaa ggagtatttc
     5401 aacctgctga ttatctgaat atcttacatt ttaattatcc tgaaacaaaa cattcaggct
     5461 acgaattgct catcaataac ttaaccgtat tttctattac caatatattt gcagtataca
     5521 ccgaacaaat tacattttgg aaagaagtgt tcccctctgc agaattcata catatgcata
     5581 cccctctcat tgatgcattg cttaaagaat ctaaaaaaga agacattcaa ctcgtatggt
     5641 tatatattca accaacaagt atttttttat cctatgcaga caaggggaaa ctcatttttt
     5701 ctaacacatt ccctattcaa tctgccacag atattgtata tgcatgtggc aatgtatttg
     5761 aacaatttgg gctaagccaa caccacacca aacttattct tactggtaat tatacatcca
     5821 aaagcgaaat atactcctta cttcacacac attttgctat gcttgatgaa tattctcttg
     5881 catcagatat aattattcaa tcttcttgca aatcatttga catacagaaa cttatggatc
     5941 tattacttgt accattatgc gtataataag tggcatatac aaaggcagaa gatttactcc
     6001 acctgcatca ctcaaagcac gaccaacaac agattttgcg aaagaaggtt tatttaatgt
     6061 gttagctaat gaaatagaca ttgaagaagc acaggtgcta gatttatttg ctggaagtgg
     6121 aagtatttct tttgagtttg tatcaagagg agcaggacac acaacaagta tagaacagag
     6181 ctttcagcat ggtgcattta taaaaaaaac ggctcaagaa ctcaatatta aaacactatc
     6241 tgtactgaaa gcagatgttt ttcgatacat agaaacctgc aatcactcgt atgatattat
     6301 ttttgcagac ccaccctata gcctgcctca attagctgaa attccaaaca aaattatgaa
     6361 ttcagcacta ttgaagccag atgggctgtt tattttagag catggtaaaa ataatacttt
     6421 tggcgaacat cccaacttta ttaataccag acattatggg aatgttcatt tttccttttt
     6481 taaacataaa acgaaataga ccaaaaactt aacgttgaaa aaaatctata taaataggta
     6541 aatgatcagc aaaccctcca atatatttca tccccaagta cgttcgaaag gtttttttac
     6601 caaaataggt ttcatcatct atcaataaaa attcagcatc aaagattttt gctgactgct
     6661 ctgatgtata aaaaaacgca tctggttgta atgcattgcc tgaaacaatc atttggtcta
     6721 atacccccca ttctgttgca tgcttatgag tacctatatt cggtttgtta tgatactgat
     6781 aatataagtt atacaaaccg tacgaacgaa taattgcatt ttgtggatct tgggcttgca
     6841 atatagaaaa taaacttgtg ttggtgggat aatcattaaa atctcccatt agtagcatag
     6901 tagaagtggg agaagccaaa aacaaactat ctacttgctt acgtaaaatc ctagacacct
     6961 ctattcgcct gtcttccgac tctagttcgc ctcctaatcg ggacgggaaa tggcacacaa
     7021 aaatatgtaa tgtatcggta tacatagcta taccttttac atacaatata tctcgggtag
     7081 tagaaaaagg tgcactagca aacgaaaccc caataggtgc agaagatagc actctaaact
     7141 gctctggacg atacaaaaga gcaacatcta taccacgctt atcaggcgaa tcgtaatgaa
     7201 catatttata attcaaattt cgtaaaggag aaaaatacac caacgatttt aataccgatt
     7261 cgttttctat ctcgcacaaa cccacaatcg atggtggagt aaattgtcca acagccgtta
     7321 taactttagc aatattggaa atttttagtt taaacttttt gggagtccaa tgataggctc
     7381 cctgtggtgt aaaatcatca tcattggtaa gagaatcatt ctcgcagtcg aatgcatttt
     7441 cgacattata acacattaca cgaaacggta tctctgcata acagggaaca atcagccatt
     7501 gagtaagcca atacatactt agcaggaagt ataaaaaaat gctcttcttc atgcctattc
     7561 agcgattata acatacgcac ctatatcagc tttgtcatta aacaaccttt gacgaccaag
     7621 catatcaata ggatattcag cagctaccag attatcagca ttacccaaag caggcgagga
     7681 gctttgcaag gtaaaatcat aattgtatgg tttcgtcgcg ttaaacttag gatcttggtt
     7741 agctattatt tccaaaaaat tggtatcatt gatagtggca ggactaattt tgattactga
     7801 atttttaaat acatacacta aaggttcttc aattttttga tttagatacg aggaactgac
     7861 agatacttca ttcgaccaac taccatatac tatactattg ataaaattgg cttcgagtaa
     7921 tggaactggc atagcttgag aaccaaggtt tatataatta gatagcagca ccgaagttgc
     7981 tttacgacca acccaaggga aggaaaaata attggcaata gtagaatgca ccacattata
     8041 ttttccaccc tgtaacacca aacaagattg ccctgcattg tatactacac tgttgcttat
     8101 atgcataacg gtatgcgatg ccgtaattaa attagtagct gaattattaa taatcgaatt
     8161 agaaatcgca agagccaaca catctaagga agtagagtca agaacaatcc cttgatttgt
     8221 atttttgaca acgacataat ttaattcgtt atgtgcactc cctttccgaa aacggatacc
     8281 ttgccactga ttgggtactt tatcgtaagg taaattatca aacatattat ccattctatc
     8341 ccctctaaaa acaacaggtt tggattgtat tcctttcgct tttagggtac catatacttg
     8401 taaactggca tgcttaacaa aataaagaga aaccccttcc gataaggtta atgttgcacc
     8461 ctgagcaatt gtcaatgtat cgagtatgag atatggtttt tcggctagcc aggtagtatc
     8521 tgccattatc tgacgtttag aaacacgata tgcatcctgt ccgtatgctt gcaacactac
     8581 cttatacgta tgcgtatttg aacgaataat gatggaatct tttactataa agggcgtaaa
     8641 ttggtcagat ggatcgacat aagcctgcac aaaaatacgc atactatcat ttgctttgag
     8701 tgaaatatca tgcaaagcat ctgttagtac accattaata tttagagaat aatgagtagc
     8761 tcctactaac tcaatagcat cgattttaat tctattttta gatggattat atatgttgaa
     8821 tattttggtt gtagatccaa ctcccgtaaa aagtgtatca aaagaaacgg tatccgcaga
     8881 aatatgtata cgttgattgg gatttgtaga aaacatcata tcttcgcatg atgaaaaacc
     8941 tatccaaacc atacaaataa gaaaaaaaaa cgagaaaaaa gttcgttgac gatacatacg
     9001 ttattcttaa tgatatagca attcgtgtac aaaattacac taatagattg ttttattgca
     9061 ataatcttgt aaatttgtgg aattattaac gaaaaaaact cctttcatat ggcaaacaag
     9121 cgtaatctta aaaaagacat tcaatccatt ttttctgaat tattagtaga gtgttacacc
     9181 ttatcttata tatttccaca aattgacatt gccaaagttg atgcaatagc aacttctatc
     9241 attgaaaaaa gtgataattt tgtaaaacgt gcaggaaaac cagatggaaa agacaatcca
     9301 gctatcgtaa aagcctatta caaaaagcta tacaaggatg tagccaatac agtatctgtt
     9361 tttatcgaag atattaacac gttaaaaagc aaataaataa gcatatttta ctattcgtta
     9421 gcaacaaact tgacacttaa attaccagag aaaaaaacaa acgaataaag cattttattt
     9481 tatgaaaaaa aatatcagca aattgttgtt aaaattaatg ggatggaaaa caacagccag
     9541 cattccctct atcaaaaaat tagtgatttg cgttgctcca catactagta attgggattt
     9601 tattattgga aaactttttt atacagcgct agggggaaaa gcttcttttc ttatcaaaca
     9661 agaatggctg aaacctcctg taggaaaatt actaatcaat atgggagcca ttggcgttaa
     9721 ccgggggaaa aacacttcgc tcacagacca attagctaaa gtttttcaag aaaaggaaga
     9781 atttcattta gcaattaccc ctgaaggaac acgaaaaaaa gtagataagt ggaaattggg
     9841 attttatcaa attgcaaaaa aagcaggagt acctattgcc atgattacaa ttgattataa
     9901 acataaagaa gtttctgtat taggcattta cgaacccact gatgatgaag aaaaagacat
     9961 taacatgata aaatcgtttt acgaaaatgc ccatgccaaa tatccctctc aattttcttt
    10021 aggtaaagat tataaacaag tatgatacaa gacaatctta cagttagcat tatccaaaca
    10081 gacattaaat gggaaaataa agcaaaaaac cttgaaatgt ttgcagaaaa agttcgagca
    10141 tgtgggtctt attccgacat catactatta cccgaaatgt ttacgacagg gttcagtatg
    10201 catgctagca acctagctga aacagtacat gatgagacaa ttaacacctt aaagaaatgg
    10261 gcattaacag aaaatgcaat gctagttgga agttttatcg ccatagacaa taatcatgtg
    10321 tacaacagag gtttcgccgt tttaccatca caagaaatat attggtacga caaaaagcat
    10381 ttattttcta tgagcaaaga agatgccgtt tttacccctg gaaatgcacc atgtattgta
    10441 ccatacaaag gatggaatat ttcattagct atctgttatg atttacgttt ccccgtttgg
    10501 ctacgtaata caaataatgc ctatgatatg ctattggtag tagccaattg gcccgaaagc
    10561 agaaaagaag cttttaatac cttactaaaa gctcgtgcta ttgaaaatag ttgctatgta
    10621 tgtgctgcta atcgtattgg cattgatgga aatcaagtgg tttatgcggg caattcgcaa
    10681 atagtagatt ttaaaggcac tatagtagca aatacaacag agcatacgga caacattgtt
    10741 tcagctactg ttaacaaaga cttgttaatt gctttcaggg aaaaatttcc tgtttggaaa
    10801 gatgctgacc catttagcct tttgtaaata caaatgcatt acttaagttt ttgaaaaaac
    10861 aatacatcct caaattcatt gccttttctt atccagtgtt ttttttcacc gacacacaca
    10921 aatcctgttt ttaaaagcat tttttgagaa gaaatattgt tttttggcac atgacaatac
    10981 acttgttcca aatcgagaaa agaaaaaaga tactccaata atagttgtat tgccattaca
    11041 gcaaaacctt tctgctgata gtgtttatct ataaatacac ccatttcagc acgtttgtga
    11101 aaaaaatcaa tttggctaac atctatggtt cctaaacaaa ctttatccct tacatgttca
    11161 atcatgaaac gcaactgctt tgtttcatac acgtcttgcg ttgtggcttg tatatattgt
    11221 tttaaggcat aacgagaaaa tggcacaata gtactcccat gttcccaata catagtatca
    11281 ttttcccact tataaaaaag gtcaagatcc tcaggctcaa tggctctaag tgtaattatc
    11341 gtattatgga gcaactcatt gttcattata cagaaaaacc aaaattagga tgatatagtt
    11401 gaatattttt ttctccttta tttttttcaa tttgttgaat aatctcagca tatgaatact
    11461 gattagtaga taacacaatt gtttcttttg ctcttctgtt acgagtgcgt tttatacgac
    11521 aacgataagc tatgctgaca tacacatgta tcaaacccga aataggataa aaccacaagg
    11581 gaaaagaccc tgcataatgt tttttataaa acaacaacat cgactcataa aatgctttta
    11641 ggtacttcat atcccctttc tttgtacttt ctcctttata atgaacaata gttgtgggaa
    11701 catagacatt tttcatccct tgattgataa tcctgtagga taaatcaata tcttctccat
    11761 acataaaaaa ctgttcatct aacaaagcct cttcttgtaa tcttttttta ttaattaaca
    11821 tgaatgcacc tgctaatata ggcacctctc caagctcatt ttctttgata ttgggataat
    11881 aatacgcatt aaaaaaggta ctacgtggga ataatttatg tataaatgtt aacttacaaa
    11941 aagctaccca cactgtagga atacctcttt tcgattcagg caaaaaagcc cctttcatat
    12001 ctaccattcg tacacctaat gctccgaaac gatcggtagt atccgctata tcgcatacag
    12061 ttttcaatgt atcctcagct ataatagtat ctggattgag caaaagaaca tactctcctt
    12121 ttgctatttt tatcgcctga ttgtttgctt tggaaaaacc aacattttcg gcattagcta
    12181 tgtaaataac agaagagaaa tatgtagaga ctaaatcgcg tgattg
//
LOCUS       DAOSRP010000055        11557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5535_length_11557_cov_5.917666, whole genome shotgun sequence.
ACCESSION   DAOSRP010000055 DAOSRP010000000
VERSION     DAOSRP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5535_length_11557_cov_5.917666"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLJ82_04715"
     CDS             <1..196
                     /locus_tag="PLJ82_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40841.1"
                     /translation="SISMNSIRFRWFCSITPNICVCTPRCTYFYYSLLFVYYNMLIIS
                     IVLFKKEQIDGTPIHLLNYA"
     gene            complement(363..803)
                     /locus_tag="PLJ82_04720"
     CDS             complement(363..803)
                     /locus_tag="PLJ82_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40842.1"
                     /translation="MKKENTQVGFALKGIKTEQFAIFEENYSPKKETSLGTELQFKLD
                     QNNKQIAVFLGFEFLQGKKVFLKIQVSCHFKIEESSWNLLIQENKLIVPKGFLAHLAM
                     ITTGTSRGVLFAKTEATPFSTFIVPTLNVAEMIKEDVLFDIAVQ"
     gene            complement(803..1264)
                     /locus_tag="PLJ82_04725"
     CDS             complement(803..1264)
                     /locus_tag="PLJ82_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR40843.1"
                     /translation="MTNKGKFLQLVSEEDTKTLAEVKKRIKNRAMLRESQQIALKVLM
                     KLDDLGWSQKDLAKAMDVTPQQISKIVSGKENLTIETQIKLQNILDIPVLASYYEHKM
                     TEMDQWIVTIEKRVEKIVAQVFEYSNNYQSTKVLKLETTDYSQENYQAIAV"
     gene            complement(1261..1791)
                     /locus_tag="PLJ82_04730"
     CDS             complement(1261..1791)
                     /locus_tag="PLJ82_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40844.1"
                     /translation="MKIVRIFADQLHLFAFCFEGEKMNELRRLLTLWNDTAYLFEFVT
                     NNKSDVPKDVPIRTLVFQLIEDANDIDDTIIEISSNPKRSLEEFFKPLDNQEYHFVEL
                     SKQKGRKNYLRLFAIKIDNNCFVITGGAIKFHHLNKDRPHTQKEMQKINRCRDYLKDN
                     GVFDADSFYEFSNEQK"
     gene            complement(1976..4819)
                     /gene="uvrA"
                     /locus_tag="PLJ82_04735"
     CDS             complement(1976..4819)
                     /gene="uvrA"
                     /locus_tag="PLJ82_04735"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR40845.1"
                     /translation="MIREDEHEQTYVEVFGAKEHNLKNVDVKIPHNSLTVITGLSGSG
                     KSSLAFDTIFAEGQRRYVETFSAYVRNFLGNIERPNVDEIKGLSPVIAIEQKTTNRNP
                     HSTVGTVTEIYDFLRLLYARAGVAYSYITGEKMVKYTEEQILSLLLQQYSGKNVYLLA
                     PVVRTRKGHYKELFEQIRKKGYLNVRIDGELREIVHGLKLDRYKNHDIEVVIDRLEVA
                     AKHEKRLKDSLHIAITQGDGIVMVLDKNSDEIRFFSKKLMCPTTGISYNEPAPHDFSF
                     NSPKGCCPKCNGVGVITKIDSSKIIPDDSLSIYDGAIAPLGKFKNGILFLQIQTLCEQ
                     YDCTLKTPIKKLPPELVDKIIYGTSDKLFIKSETAGLSNYPIAFDGIVHYVTMQQDSD
                     ASATAQKWAEQFSETVVCPECNGTRLKKEALFFKILDKNIAELSAMDIEQLHSWIQLN
                     TQHFDERQKVIATEICKEITTRLQFLLNVGLTYLSLNRPSMSLSGGESQRIRLATQIG
                     SQLVNVLYILDEPSIGLHQRDNLKLIDSLKQLRDMGNTVIVVEHDKDMMLAADYVVDL
                     GPKAGRLGGELVFAGTPSEMLRAHTLTSDYITGVRKIEIPPKRRDGNGNYLRIKGAKG
                     NNLKGVDVSFPLGMFICVTGVSGSGKSSLINGTLYPILSQHFYHSLKNPLPYDTVEGL
                     EHIDKVIQIDQSPIGRTPRSNPATYTGIFSDIRNLYVNLPEAQIRGYKPGRFSFNVKG
                     GRCETCKGNGYNRLEMSFLADVYVPCPDCHGKRYNRETLEVRFKGKSIADVLNMTINQ
                     AVEFFDNIPTIVHRLRVLQDVGLGYIKVGQPSTTLSGGESQRVKLATELAKKDTGQTM
                     YILDEPTTGLHFEDINVLLGVLNRLVDKGNTLVVIEHNLDVIKCADHIIDIGLEGGEK
                     GGTILCQGKPEEVVQQNCGYTVPFLKEELLAAR"
     gene            complement(4816..5571)
                     /locus_tag="PLJ82_04740"
     CDS             complement(4816..5571)
                     /locus_tag="PLJ82_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40846.1"
                     /translation="MQHIKPYILFFVFLLVFFSCTKEKTIDTPPTHETNSITQTNVLE
                     KVLGTYSLFDRNWNLMEYTIFQEDSVFKAKITYNKKTSVTLLRLVDLSSNLYSFEYDG
                     KTYYLCVDNILAEKRTIRILNEREERFLNFFEGKGFVDLSEQENRKNQTLRRKLDISS
                     FFSPIRPTKKNWSSDMNYTDTLQYISFDDVSNPMRAIFTTNTRDTVYLSFEIPIGSEF
                     NGSTMEVRWKIDTVMVNKLPKLNQKLLHYRVVR"
     gene            complement(5576..6313)
                     /gene="pssA"
                     /locus_tag="PLJ82_04745"
     CDS             complement(5576..6313)
                     /gene="pssA"
                     /locus_tag="PLJ82_04745"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOR40847.1"
                     /translation="MSFTIQKHIPNTITCFNLFSGTIAVSFAFEGNLLFATLFIFLSA
                     VFDFLDGFLARLLGAYSELGKQLDSLADLVSFGLAPSVIVFSLFKEFPYPEVMFDYAF
                     LFPYTGFFIVVFSALRLAKFNIDESQTTSFRGLPTPANAILIGGIALSQFSFWTIPVV
                     GLYLLLALVCVSCFLLVSPLPMFSLKMKGFSIANNKVQYTFLLLSLVLLVAFFFFGFA
                     LSICLYILMSVVLAIKNRLSNKKDLHI"
     gene            complement(6326..7732)
                     /locus_tag="PLJ82_04750"
     CDS             complement(6326..7732)
                     /locus_tag="PLJ82_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOR40848.1"
                     /translation="MLLLLLYVPVYATESTCVSGTVSGYPGTTIELYYFTDEISYKQE
                     VLTKTSIDSAGVFEFVFTLDKVRKCFVDLGAFRGILFIEPSKTYHLQPMPYQPKTDAQ
                     LLNMYFTPQEVLVGLQNQPPDDINQRILQFELAIDEVWNNAMYTDLSKRYLSAVIDSL
                     EMVFPSKNEFFSIYKQSTYALLANLQVELAPSWSIQDYFISMPVHYHNPAYCEAFTVL
                     FEGYFNLLESQPNAKAFFQAYHNADIVSLREQFALLTHWNNPQLEELVILYNLWGNFS
                     LKPKDRTTIINLLEQINHTSLYPENKKLAQEVLYRLKKAHVGSKPNVNELRTIEGKTI
                     TFSDFAGKYVYIVFASSLINETYADIIYLDKLRKKYAENLEVVFIFAQETEAKTIAYT
                     KEIGADLIFCNWNSDAQLIDAFGVQNIPSYYLLDREGFFSHSPALSPSQGFERKLDAM
                     LEVEKAAEGSSTMNKPFF"
     gene            7827..8879
                     /gene="mnmA"
                     /locus_tag="PLJ82_04755"
     CDS             7827..8879
                     /gene="mnmA"
                     /locus_tag="PLJ82_04755"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR40849.1"
                     /translation="MYTKENVLLGMSGGTDSSVSAFLLQQAGYNVIGVTFRFYEHNDD
                     QTYLQKASNMAKHLHIPHYIVDKRKDFQSIVIENFTNEYLAGRTPFPCAHCNTYLKWD
                     ALLEQAHRLDCPHIATGHYVNTIKKDNCTYLSMGKDIDKDQTFFLWGMPQEAIERAIF
                     PLGNLLYHEVQNIATSNQLPVKQNKNSVGACFCPTDYRPLLKKIATEKGIQIHQGIFT
                     DEIGNFLGKHEGYPFYTIGQRRGLGLQHISPLYVKKINRETNSIIVAPKESCYQTTVH
                     LTGLICRQLVEKEKQLTAKVQYKKQACTATLDMINTSEALVTLHEPTLGMAPGQSIVL
                     YDGDILIAGGIIQNAW"
     gene            8944..9016
                     /locus_tag="PLJ82_04760"
     tRNA            8944..9016
                     /locus_tag="PLJ82_04760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8977..8979,aa:Phe,seq:gaa)
     gene            9137..10510
                     /locus_tag="PLJ82_04765"
     CDS             9137..10510
                     /locus_tag="PLJ82_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOR40850.1"
                     /translation="MSINKYNNIFFIGVAGVGMSAIAQYLQGIGKQVSGSDRYFVADQ
                     PNDTKNKLEAEGIHCFPQDGTGISDITDLVVVSTAIEDTVAEVQKAKQLNIPILKRSQ
                     VLAMIAESKKTIAVGGTSGKSTVSAMIFDTLEFAGMEPSIISGAGLTSIIREGKIGNA
                     KVGKGEWLVIEADESDGSIVQYHPEIGLLLNIDKDHQEIDELMEIFGIFKQHTKSRFI
                     TNQSNKLAAQLSANLAHDFASSPSEKAGYVGSDFSQNGFEISFTINGKPFKMNAIGKH
                     SMENALATTAVCHQLGVSLETIAAALVQYEGIYRRHQVIGKKRGVTIIDDYAHNPAKC
                     AASISACQPISPKVIAWFQPHGYGPTRFLRADFVAEIAHVLRENDEIWMSEIFYAGGS
                     AVKDISANDLIEDIQKLGKKAYFVANRKDFVTAIQPHLSENTVLLLMGARDPSLETFA
                     KEVLEGL"
     gene            10533..11459
                     /locus_tag="PLJ82_04770"
     CDS             10533..11459
                     /locus_tag="PLJ82_04770"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOR40851.1"
                     /translation="MKIAIIGTGGVGGYFGARLLEAGFDVSFLARGEHLEAIKKNGIQ
                     LKSLLGDIQTKPCKASDKISDLGVSDVLIIGVKAWQIKEIRTELQSIMHKDSIVLPLQ
                     NGVMAADELSETIDKQHIVGGLCRIVSMITAPGCIAHTGVTPTIVFGELDHTISTRLQ
                     NLQENFKKANIHCQLSTNIETEIWKKFLLICISGLMAVTRCTYGEMNEVELTRQMMFK
                     LLEEIYAVGMAKGIQYEPDIVARTVASFDALPYNTTFSLTRDVWEGKPSELEYQNGTV
                     VHIGKQVKVPTPINEFIYTSLLPLEKKARTLK"
BASE COUNT         3711 a         2213 c         1963 g         3670 t
ORIGIN      
        1 ttcaatctct atgaattcca ttcgtttccg atggttttgt agcattacgc ccaacatctg
       61 tgtatgtaca ccaaggtgca cctatttcta ttatagttta ttatttgtat attataatat
      121 gctgataatt agtattgtat tgtttaagaa agaacaaatt gatggtacac ctatccattt
      181 gttaaattat gcttaaatct ctacttttct caatttcctc gaaacacttt atttctgtaa
      241 taatctcgta aagctactaa agatttttgg atttaaaaaa cttgaagtgt gttttttttt
      301 catcaaagtg ttgttacaaa caacaaacga tatcctcctc gttttcaacg aggaggatat
      361 cgttattgca ctgcaatatc aaacaaaaca tcttctttaa tcatttctgc cacatttaaa
      421 gtaggaacta taaaggttga aaatggcgtt gcttcagttt ttgcaaacaa aactcccctt
      481 gatgtgcctg tcgttatcat tgccaagtgt gctaaaaaac cttttggcac aatcaattta
      541 ttttcttgaa ttaacaaatt ccaagaactt tcttctattt taaaatgaca actaacttga
      601 attttaagaa atactttttt cccttgcaaa aattcaaatc cgagaaagac agcaatttgt
      661 ttgttatttt ggtctagctt aaattgcagt tctgtaccca aactcgtttc tttttttggg
      721 gaataatttt cctcaaaaat ggcaaactgc tcagttttta ttccttttag agcaaaacca
      781 acttgtgtat tttccttttt cattatactg cgattgcttg ataattttct tgtgaataat
      841 cagttgtttc taacttcaat accttagttg attgatagtt attgctatat tcaaatactt
      901 gagcaacaat tttctcaact cttttttcaa tggttactat ccattgatcc atttcagtca
      961 ttttatgttc ataataactt gctaatacag gaatatctaa gatattctgt aacttgattt
     1021 gggtttcaat ggttaaattt tctttgccac ttacaatttt tgaaatttgt tgtggcgtaa
     1081 catccattgc ttttgcaagg tctttttgtg accaccctaa atcatctaat ttcattagca
     1141 ctttaagggc aatctgttgc gattcacgca acattgcacg gtttttaatt ctttttttta
     1201 cctctgctaa tgtcttagtg tcttcttctg aaacgagttg aagaaatttt cccttatttg
     1261 tcatttttgt tcgtttgaaa attcatagaa agaatcagca tcaaatactc cattatcttt
     1321 caaatagtct ctgcacctat ttatcttttg catttccttt tgtgtatgtg gtctgtcttt
     1381 gtttaagtga tgaaatttga ttgcaccacc tgttattaca aaacaattat tgtcaatttt
     1441 gattgcaaat aatctcaaat aatttttcct tcccttttgt tttgaaagtt ccacaaagtg
     1501 gtattcttga ttgtccaaag gtttgaaaaa ttcttccaaa cttctttttg gattgcttga
     1561 aatttcaata atggtatcat cgatatcatt tgcatcttca ataagttgaa aaactaacgt
     1621 acgtattggg acatcttttg gaacatctga tttgttgttc gtaacaaact cgaataagta
     1681 tgctgtatcg ttccataatg ttaataatct tcgcaactca ttcatcttct cgccttcaaa
     1741 gcaaaaagca aataagtgta attggtctgc aaagatacga actattttca taaaatcaac
     1801 ctataggttt atatttaaca tttttatttc tctctagtca ttattttcca cgcttactcc
     1861 taacaataat ctttctaata aacactctgt ttagtttagt ttagtttttg ctcaaatgta
     1921 atgcatacaa accaaaaact cctcgctgaa aacgaggagt agagaaaaaa ccacatcatc
     1981 gtgcagccaa taattcttct tttaaaaacg gaacggtgta accacaattt tgttgtacta
     2041 cttcttcggg tttcccttgg cacaaaatag taccgccttt ttctccacct tcaagtccaa
     2101 tatcaatgat atgatcggca cattttatca catctagatt atgttcaatt acaactaagg
     2161 tattgccttt gtcaaccaat cgattcagta cgcctagtaa cacattgata tcttcgaaat
     2221 gcaaacctgt agttggctca tccagtatat acatggtttg tcctgtgtct ttttttgcca
     2281 attcggtggc tagctttact cgctgacttt cgcctcccga taaggtggta gagggttgcc
     2341 ctactttgat ataacctagt cctacatctt gcaacacacg caagcgatga acgatggttg
     2401 gtatattgtc aaaaaattct accgcctgat taatcgtcat attcaagaca tctgcgatgg
     2461 atttcccttt gaaccgcact tccaatgttt ctcggttata ccgttttcca tgacaatcag
     2521 gacaaggcac atacacatct gccaaaaaac tcatttctaa gcgattatac ccgtttcctt
     2581 tgcaggtttc gcacctacct ccttttacgt taaacgaaaa acgacctggt ttatatccac
     2641 gtatttgtgc ttcaggcaaa ttaacataca aattgcgtat atccgaaaat atacctgtat
     2701 aggtagctgg attagaacgt ggtgtacgtc cgataggcga ttggtcaatt tgtataactt
     2761 tatcaatatg ttccaatccc tctacagtat catacggtag cggattttta agagaatgat
     2821 aaaaatgttg gcttaaaatg ggatacaagg taccgttaat taagctagat tttccgcttc
     2881 ccgatacacc cgtaacacaa ataaacattc ctaatgggaa agacacatca acccctttta
     2941 ggttattccc ttttgctcct ttaattcgca aataattgcc gtttccatcg cgtcttttag
     3001 ggggtatttc tattttccta actcctgtaa tataatcaga agttaaggta tgtgcacgca
     3061 gcatttccga gggagttcct gcaaaaacca actcaccacc taatcgtcca gctttgggac
     3121 ctaaatcaac cacataatcg gctgccaaca tcatgtcttt gtcgtgttct acaacaatga
     3181 cggtatttcc catatcacgt aattgcttta gcgagtctat cagttttaaa ttatctcgtt
     3241 ggtgcaatcc aatactgggc tcgtctaaaa tatacagcac attgactaat tgagacccaa
     3301 tctgcgttgc caaccgtatg cgttggcttt cgcctccaga caaactcatg gaaggacgat
     3361 taagagagag gtaagtaagt cccacattta ataaaaactg taaccttgtg gtaatttctt
     3421 tacaaatttc tgtggcaatt actttttgtc tttcatcaaa atgttgtgtg ttgagttgta
     3481 tccaactatg caattgctca atatccatag cagacaattc agcaatattt ttatccaaaa
     3541 ttttgaaaaa caaggcttct tttttaaggc gtgtaccatt acactctggg catacgactg
     3601 tttctgaaaa ttgttcagcc cacttttgag ctgttgcaga ggcatcgcta tcttgttgca
     3661 tggttacgta gtgcacaatg ccatcaaagg caataggata attagataag cctgctgttt
     3721 cgcttttgat aaataattta tcggatgtgc cataaataat tttatccact aattctgggg
     3781 gcaatttttt tataggtgtt ttcagcgtac aatcgtattg ttcgcataat gtctgaattt
     3841 gtaaaaataa tatcccattt ttgaattttc ctaagggtgc aatggcacca tcataaatag
     3901 aaagagagtc gtcaggaata attttactac tatctatttt ggtaataaca cctaccccat
     3961 tacattttgg gcaacagcct ttgggcgaat taaaagaaaa atcgtgagga gcaggctcat
     4021 tgtacgaaat tccagtagtc ggacacatta attttttgct aaaaaacctg atttcatcgc
     4081 tatttttatc taataccata acaattccat cgccttgggt tatcgctata tgtagcgaat
     4141 ctttcaatct tttttcgtgc ttggcagcta cttccaatcg gtcaatgacg acttcaatat
     4201 catgattttt gtatctatca agttttaatc catgtacaat ttctctcaat tcgccgtcta
     4261 tccgcacatt caaatagccc ttctttctta tttgttcaaa caactctttg taatgtcctt
     4321 tgcgagttct aacgacagga gctaacaaat acacgttttt cccactatat tgctgtaaaa
     4381 gcaatgaaag aatctgctct tcggtatatt ttaccatttt ctcgccagtg atatacgaat
     4441 acgccacacc agctcgtgca tacaaaagac gtaaaaaatc gtatatttcg gtaaccgtac
     4501 ccacagtaga atgtggattg cgattggttg ttttttgttc tatggcaata accgggctca
     4561 aaccctttat ttcatcaaca ttcggacgtt cgatattgcc caaaaaatta cgtacatagg
     4621 cagagaatgt ttctacatag cgtctttgtc cttccgcaaa tatggtatca aacgccaaag
     4681 aagatttacc acttccgctc agtccagtaa ttacagttag gctattgtga ggtattttaa
     4741 cgtctacatt tttgaggtta tgttctttcg ctccaaaaac ttctacatac gtttgttcat
     4801 gttcatcttc tcgtatcatc gtactactct ataatgcaac aacttttggt ttaatttagg
     4861 taatttattc accatcactg tatctatctt ccacctcact tccatcgtac ttccattaaa
     4921 ctccgaccca ataggtatct caaagcttaa ataaacagta tcccttgtat ttgttgtaaa
     4981 aatagctctc atgggattgg atacatcatc aaaagaaata tactgtaagg tatcagtata
     5041 attcatatca ctactccaat ttttctttgt tggacgaata ggagagaaaa aagaagatat
     5101 atctaatttc ctgcgtaacg tttgattttt cctattttct tgctcactca aatcgacaaa
     5161 acctttccct tcaaagaaat ttaaaaaccg ctcttccctt tcattcaaaa ttcgtattgt
     5221 tcgtttctca gccaatatat tatctacaca taaataatag gttttaccgt catattcaaa
     5281 tgagtacaaa ttgctcgaca aatctactaa ccgtaaaagc gtaacagatg tttttttatt
     5341 atacgtaatt tttgccttaa atacggaatc ttcttgaaaa atagtgtact ccatcaagtt
     5401 ccaattccta tcaaacaacg aataggttcc tagcaccttc tccaacacat ttgtttgtgt
     5461 aatactgttt gtttcgtggg ttggtggtgt gtctattgtt ttttctttgg tacaagaaaa
     5521 aaacacgaga agaaacacaa aaaatagtat atatggtttt atatgttgca tcaatctata
     5581 tatgtaagtc tttcttattc gatagacgat tttttattgc aagtacaact gacataagga
     5641 tatacaaaca gatagacaac gcaaagccaa agaaaaagaa tgcaacaagt agcacaagtg
     5701 aaagcaataa aaaagtatat tgcaccttat tgttagcaat actaaatcct ttcattttga
     5761 gagaaaacat aggtaaaggc gatactagta aaaaacaaga tacacaaacc aacgctagta
     5821 atagatataa acccacaaca ggtattgtcc aaaatgaaaa ttggcttaac gcaattccac
     5881 caattaatat cgcattcgct ggcgtaggca agcctctaaa actggttgtt tgagactcgt
     5941 caatattaaa cttagccaat cgtagtgcag aaaatacaac aataaaaaaa cctgtataag
     6001 ggaataaaaa agcataatca aacattacct ctggatacgg aaattcttta aacaaagaaa
     6061 acacaataac actaggtgct aatccaaagc tcaccaaatc ggctaacgaa tcgagttgtt
     6121 tgcctaattc tgaatatgct ccgagcaaac gagccagaaa accatccaga aaatcgaata
     6181 cagctgaaag gaaaataaat aatgtagcaa acaataaatt accctcaaaa gcaaacgata
     6241 ctgcaatggt accagagaat aaattgaaac acgttatcgt atttgggata tgtttttgaa
     6301 tagtaaatga catatggtat aatgattaaa aaaatggttt attcatagta gacgaaccct
     6361 cagcagcttt ttctacttcc aacatagcat cgagtttacg ctcaaaacct tgcgatggac
     6421 ttaatgctgg actatggcta aaaaaacctt ctctatccaa caaataatag gaaggaatat
     6481 tttgcacacc aaatgcatct atcaattggg catcactatt ccaattgcaa aaaattaagt
     6541 ccgcacctat ttcctttgta taggctattg ttttcgcctc agtttcttgt gcaaagataa
     6601 atacaacttc taggttttca gcatattttt tacgcaattt atccaaataa ataatatctg
     6661 cataggtttc gtttatcaac gaactagcaa aaactatata cacatacttc cctgcaaaat
     6721 ccgaaaacgt aattgttttt ccttcaatag tacgtaattc attcacattg ggcttacttc
     6781 caacatgggc ttttttcaat ctatacaaca cttcctgtgc caatttttta ttttctggat
     6841 acaagcttgt atggttgatt tgttccaata aattgataat agttgttcta tctttcggtt
     6901 ttagagaaaa atttccccat aagttataca aaattacgag ctcctctaat tggggattat
     6961 tccaatgcgt aagcaatgca aattgttccc taagcgatac aatatctgca ttatgatacg
     7021 cttgaaaaaa tgctttagcg tttggttgag attcaaggag gttaaaatat ccctcaaata
     7081 acactgtaaa tgcttcacaa tatgctggat tatgatagtg tactggcatg gatataaaat
     7141 aatcttgaat actccaactc ggagctaatt ctacttgtaa atttgctaat aatgcatacg
     7201 tactttgttt gtatatagaa aaaaactcat tcttactagg aaagaccatt tcaagactat
     7261 ctattacagc agaaagatat cttttagaaa ggtcggtata catggcatta ttccatacct
     7321 catcaatagc tagttcaaat tgaagaattc gttgattaat atcatctgga ggttgatttt
     7381 gcaacccaac aagtacctct tgtggtgtaa aatacatgtt taacaactga gcgtccgttt
     7441 tgggctgata gggcatcggt tgcaaatggt acgtttttga tggttcgata aacaaaatac
     7501 ccctaaaagc acccaaatcg acaaaacact tacgaacttt atcaagggta aaaacaaact
     7561 caaatacacc tgcagaatca atgcttgttt tcgttaaaac ttcttgttta tagctgattt
     7621 catcggtaaa atagtacaac tctatcgtag tacctggata tccagatacc gtgccagaca
     7681 cacacgtaga ttcagttgca tatacaggca catatagcaa caaaaggagc ataaaaacca
     7741 gcatattttt tttcattccg taaaaataca aaaagatata gacaaaaaaa taatttaacg
     7801 tacttttgta caaagttaac atgtttatgt atacaaaaga aaatgtttta ctaggaatga
     7861 gcggaggaac ggatagttct gtatcagcct ttttactcca acaagcaggg tataatgtta
     7921 ttggcgttac tttccgtttt tacgagcata acgatgacca aacctaccta cagaaggcta
     7981 gtaatatggc aaaacaccta cacataccac actatattgt tgataaacgc aaggacttcc
     8041 aatcaattgt aatagaaaat ttcacgaatg aataccttgc agggcgtacc ccttttcctt
     8101 gtgcccattg taatacatat ctgaaatggg atgccttact cgaacaagct catcggttag
     8161 attgccccca tattgctact ggacattatg tgaatacaat caaaaaggat aattgcacct
     8221 acctctctat ggggaaagat attgataaag accaaacttt ttttctttgg ggcatgcctc
     8281 aagaagccat cgaacgagct attttccctt tgggtaattt gctctatcac gaagtgcaaa
     8341 acattgctac ctccaaccaa cttccggtga aacaaaataa aaacagtgta ggtgcatgtt
     8401 tttgtcctac ggactaccga cctcttttaa aaaaaattgc aacagaaaaa ggaatacaga
     8461 ttcatcaagg aatatttacc gatgaaatcg gtaattttct agggaaacac gagggttacc
     8521 cattttacac cattggtcaa cgtaggggtt tgggtttgca acatatctcc cctttgtatg
     8581 taaaaaaaat caatagagaa accaattcca ttattgtagc accaaaagaa tcgtgttacc
     8641 aaacgaccgt acatttaaca ggattaattt gtcgccaact tgtagaaaaa gaaaaacagc
     8701 taacggcaaa agtgcagtat aaaaaacaag catgcactgc tacattagac atgataaata
     8761 cctcggaagc cctagttacc ttacacgaac ctacactcgg aatggctcca ggtcaatcga
     8821 ttgttttata cgatggagat atacttattg ctggtgggat tattcaaaac gcatggtaaa
     8881 catatttttt ttataaaaat atttggtatt tatcaaaaag gtattacctt tgcacccgca
     8941 actggtacca tagctcagtt ggtagagcaa aggactgaaa atccttgtgt ccccggttcg
     9001 attcctggtg gtaccacaac taaaagccca aagttaactt tgggctttat ttttgcctat
     9061 actttagtgg aatttacatt gttgtaatgt aaaaaattac tacttttgga aatcattagc
     9121 atttaacaaa aagagtatgt ctataaacaa gtacaacaac atttttttta ttggagtagc
     9181 cggtgttggc atgagtgcta ttgcacaata tctacaaggt attggcaaac aagtgagtgg
     9241 tagcgatagg tactttgtgg cagaccaacc caacgacaca aaaaacaaat tagaagctga
     9301 aggaattcat tgttttccgc aagacggaac gggaatatcg gatattaccg atttggtagt
     9361 ggtttctacc gctattgaag atacggttgc tgaggttcaa aaagcgaaac aactaaacat
     9421 ccccatactc aaacgctcgc aagtactagc tatgattgcc gaaagcaaaa aaaccattgc
     9481 agtaggtggc acatcgggaa aaagtacggt ttcggcaatg attttcgaca ccttagaatt
     9541 tgcaggaatg gaacctagca tcattagtgg tgcaggctta acgagtatta ttcgtgaagg
     9601 gaaaataggt aacgccaaag taggaaaagg cgaatggctg gttattgaag ctgacgaaag
     9661 tgacggttcc atcgtacaat accaccccga aataggtcta ttactcaata tagacaaaga
     9721 tcatcaggaa attgatgaat taatggaaat atttggcatc ttcaaacaac acaccaaatc
     9781 acgttttatt acgaatcaat cgaacaaatt ggctgctcag ttgtcggcaa acttagcaca
     9841 tgattttgcc tcatcgccca gcgaaaaagc aggctatgtg ggtagcgatt tttcgcaaaa
     9901 tggctttgaa atcagtttta ctatcaatgg aaagccgttt aaaatgaatg ccataggcaa
     9961 acatagcatg gaaaacgcct tggctacaac agccgtttgc catcaattag gggtaagttt
    10021 agaaacaatt gcagccgcgt tggtgcagta tgaaggcatc tatcggcgac atcaagtaat
    10081 tggaaaaaag cgaggtgtta ccattattga cgactacgcc cacaacccag ccaaatgtgc
    10141 ggcttccatc tcggcttgtc agcccatttc gcccaaagta attgcgtggt ttcagccaca
    10201 tggatatgga cctacccgat ttttgcgtgc cgattttgtg gcagaaatag cccacgtgtt
    10261 acgtgaaaat gatgaaatat ggatgagcga aatcttttat gcaggaggtt ctgcggtaaa
    10321 agatatttcg gcaaacgact tgattgaaga tatccaaaaa ttggggaaga aagcttattt
    10381 tgtggcaaac agaaaggatt ttgttacagc aattcaaccg catctctccg aaaacacggt
    10441 actcctactg atgggagcac gcgacccaag cctagaaacc tttgccaaag aggtgctaga
    10501 gggattgtag aaaattataa aataaattta ccatgaaaat agcgataata ggtacaggag
    10561 gcgtaggcgg atattttggt gctcggctgt tggaggcagg ttttgatgtg tcgtttttgg
    10621 caagaggcga acatttggag gcaattaaaa agaatggaat acagctaaaa agcttattag
    10681 gagacatcca aactaaacca tgcaaagctt ctgataaaat cagcgattta ggcgtttctg
    10741 atgtactaat tattggcgta aaagcatggc aaataaaaga aattcgcaca gaattgcaaa
    10801 gcattatgca caaagatagc atcgtattgc ccctacaaaa cggagtaatg gctgcagacg
    10861 aactgagtga aacgattgac aaacagcata ttgtaggcgg actttgccgt attgtaagta
    10921 tgattacagc tcccggttgt attgcccata cgggagtaac acctactatt gtgtttggag
    10981 aattagacca tacaatttcg acgaggttgc aaaatttgca agaaaatttc aaaaaagcca
    11041 acattcattg tcaactttca accaatattg aaacagaaat ctggaaaaag tttttgctga
    11101 tttgtattag tgggttaatg gctgttacaa gatgtactta tggtgaaatg aatgaggttg
    11161 agttaacgag acaaatgatg tttaagttac ttgaagaaat ttatgcagta ggcatggcaa
    11221 agggcattca atatgaacca gatattgttg caagaacagt ggcatctttc gatgctttac
    11281 cctacaatac aacgttttcg cttacacgag atgtatggga aggaaaaccc tcagagcttg
    11341 aataccaaaa tggtactgtt gttcatatag gaaaacaagt aaaagtacct actcctatca
    11401 acgaatttat ttataccagc cttttaccct tagaaaaaaa agccagaacc cttaaataat
    11461 agacttttag gtaaattttt aaggtgattt ttgccccttc catttagttt ttgtaccttt
    11521 gcaccctcaa aattaatagg aagtgtattc aaactta
//
LOCUS       DAOSRP010000056        11388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5622_length_11388_cov_5.284479, whole genome shotgun sequence.
ACCESSION   DAOSRP010000056 DAOSRP010000000
VERSION     DAOSRP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5622_length_11388_cov_5.284479"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..671)
                     /locus_tag="PLJ82_04775"
     CDS             complement(78..671)
                     /locus_tag="PLJ82_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HOR40852.1"
                     /translation="MSCSKLNYSPIVPNYYDTSQSTIYYSGNWNSISNAKLIEYLFKA
                     FDISESQISKDEFVSILLRNIPAKYEMPPTPPPIIPDGSTGRFGEELVYKELVAKFGE
                     SRVNWLNKGGETYKEYDFEILSRNNEILYFIDAKATTTGEVTGDTVPIYIRPSEWAFM
                     QRCKDNYIIARVYNAKTTSAHTKYLKMGLQNLQEINL"
     gene            complement(746..4717)
                     /locus_tag="PLJ82_04780"
     CDS             complement(746..4717)
                     /locus_tag="PLJ82_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR40853.1"
                     /translation="MLPAILRNNTTTMKNFQEQIKQFFAKTDKEILDYLLQWWEMGSV
                     SENGTIRLIGTYEKTEKPDKNGNEYGFFVNVRSPKGDILYYPFRLGQVRIYSPHKEHY
                     QNNKSGFHQLEVSLSNVKFRKDKDNPFMLNLVKHLGEPKSTFFDRLEKEKFIKDLFSK
                     RGATPDDARTIANALSKLAGDLYTDLTERFVFELLQNADDMPNEKNEVNVKFILLQEN
                     LLFVHNGKPFDQADIRAITDIGYSTKKKDPEQTGYKGIGFKVVFRESDNVLIKSGGYS
                     FSFDKEHPFYNQLQSSLYEGLNIDEIPWQLKPIWAENYRYSREIKECEDFMKNSPVAI
                     AIETDFQKIKLYKSQIKALFNEPRFSLFLRKIKTIEVLGLESPILIEKQKSSKGENYF
                     VLNDNEWIINDFEFGVTPEIREGMANDKIVPEKLKEIQKSKLSFACQIKDSKIESISP
                     DKSFLFTYLPTNVNDYKFPFLVNADFLTTANRQSIHVKNIWNLYLFEQIGFLSFKWIS
                     EIAQNEDLRFTITNLVPSRFQNSAEPIHQYFNNGFDKAIKEIAFLPTANGALCKVSDA
                     LVDLTGLSEIIGKELFVRVVNPSKTLIDNRLEFKSKLIGLQGISIFGKDELKKIFANA
                     SFQQALTPQLLLEVLKFLQEKEYNFSDIGLLYSENSSQGLFTPSSMYFQTNHADKSLL
                     TFKAVYFLHPTINDFADSDTKFKNWLTSLGVKAFEGRSFISQNILNFSNDINSKITDK
                     TNNLSFWRFVFKYELLDSEVQKLAPYYVFNTSNVLVSNLDTCYLSDFYKDSGEPSVQQ
                     IAADLGLTDFNFISSDYCQNASDKSKWRKLFYGAGLKHSQNVRIFKDKIVPFIQNGKM
                     DANNYLKITKFVFEVFNDTQNRSSFDVNLSNFQVLTTSGHLQPILSCILSDDYTQDLR
                     LASVLPEQVLPNQIHSVYLQQISNNRQNWKEFFLRLNSNVELTSTDIVKRKINILASN
                     PSLITHQNVQQVWKTVLSFKEDLLKTHKEELKKIPLLLKNNSLTVPTVCYFPKEYSPN
                     TEIEDLLTGYYDYFISPIFNSISGLSYSDLKTFFKQIGVDEEIRRSNVGNSYFDVSHK
                     EHLSKFDFAKKFWTYFQKNQSLFTINTSSTFKTYIQSNPSIPCLDNAVRTPNIVHSYR
                     LKDLVNDSSVTCGIEFSPELETFLGLQQRLTVSKCFQILNSIAVSNGTDDKKIRLIYD
                     DLLYRFSNESLSSFSTDIANFKQNGKLLSNKDTFQSVTSLFYQDVSANYLPLDESDKV
                     VKRFGDKDYWKKFEVVLKQLGIQIITVSDFSLDAQSSKYDAVELSKTLNNSIAELRKK
                     LIQ"
     gene            complement(5058..5897)
                     /locus_tag="PLJ82_04785"
     CDS             complement(5058..5897)
                     /locus_tag="PLJ82_04785"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOR40854.1"
                     /translation="MKKLFVYILLFVLPIGNIHSQETNSCGITNTSFKAGEKLRYSAY
                     FRWGILQLSSFSIQSEVKRERYKGESVFAITTTGVTAGNMDKFFTIRDTLHAKLKVND
                     LQPLYFKEIANEEKFKATREFKYDYTDSTRVSWQFADNKNKEKAGNTTLTNCAYDAVS
                     IIYRLRNYDFNAETLKPNQTFRIDYFSDGKSSPLQMRFEKKEIVTLKNGDRYDCLKFE
                     LEVAEGTLFKNNERVFIWITNDKNRLIAYVEAKLKIGYVKMDLVNVEHHRHPLTAKLE
                     PVR"
     gene            complement(5901..7703)
                     /locus_tag="PLJ82_04790"
     CDS             complement(5901..7703)
                     /locus_tag="PLJ82_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOR40855.1"
                     /translation="MISTLIKRILLISAITVSVTLLLHSCANRGAGPQGGIRDTTPPQ
                     LLKSSQSNKATSATNQRLEWIFDENIQLEKQQENILVSPPQKQAPTIRAMGKKLSVAF
                     ADSLASNTTYTIDFGNAVVDLNEKNALANFSWSFATGATIDTMQLAGYVIDARTLTPY
                     EGIIVGVYKSNKDSAFVSTPFDYMARTNSQGFFTIRNIPQVPYQVYALKDIPQAFYYD
                     QKGKEIGFFSQKVTPEEVKHLQTDTIWNDNDTVSILRIDTSYSISYAPNDLLIKLFRE
                     ERAMIPTLRRALRPSKESFTLLMSAALDKEPLITPINVETSKWLKREQTNRMDSLVYW
                     ITDSNALALDTIQFTVSYASADSLKTDTIALTYKQPRARTANEKTLQQLEIVSLTKSP
                     VDIADTLRLQVKEPMKSLNIEKMHLTMRIDTTWHNVPFSLQYDDDNCKKKISVLFDRK
                     ERANYRLVIDSAAIVSDYGKQNTSFVQTFTTKSPDDYASLMIEWKNIPPYGIVELLDA
                     KETVVRAVHVSEPTTVLRLLSPGNYFLRLVLDANQNGKWDTGSVLPKLEPEEVYYYPK
                     RLVLRANWDSVEEWDYTAIPLLQQRATELPKSKK"
     gene            complement(7700..8980)
                     /locus_tag="PLJ82_04795"
     CDS             complement(7700..8980)
                     /locus_tag="PLJ82_04795"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOR40856.1"
                     /translation="MKEELLHHIWKYRLFASHQLTDTEGSTIDIIDVGHHNLDAGPDF
                     VNAKIKIGDTLWAGNVEIHKKASDWNTHKHPTNPAYNSVILHVVAECNTDIFTQEGRK
                     IPQLKLPLTPALLEQWQQLEHSPHKIACHLHLHRISDMIKTSWLEALVIERLETKASR
                     IDTLLEQNKNNWEETFYQTLARNFGFGLNSDPFEWLAKSLPINYIAKHKNQLLQIEAL
                     LFGQSGLLQLSQATDDYTVQLKNEYAFLQQKFSLTPIDGSLFKMMRLRPANFPHIRLA
                     QLAQLIHTSSKLFSHIVENPTYSSLKKMFAVSTSPYWETHYTFGEVSPRKTKKLGEKA
                     INSIIINTIVPILFAYAKRKNNDELQEKSVHLLESCAAEDNSIVRNWQEMGFTATSAY
                     QTQALIQLQKQYCQTKKCLYCRFGHKLLQLSLQP"
     gene            complement(9094..10644)
                     /locus_tag="PLJ82_04800"
     CDS             complement(9094..10644)
                     /locus_tag="PLJ82_04800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOR40857.1"
                     /translation="MASQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVEL
                     KNNIKKYWWDSMVLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRA
                     DVLIEDLMAKYDDKINKEIEKAAKKFGDAFDATLFAETNPRIQENKAKKEEIHTRFAK
                     ALNDGDLNELKQIIVDYDVVCPISGTKNWTDVRQFNLMFSTEMGSTADGAMKIYLRPE
                     TAQGIFVNYLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWFEHWKQFRLRWHKALGLGDHKYRFHDHDKLAHYANAATDIEFELPFGFKEIE
                     GIHSRTDFDLGSHEKYSGKNIKYFDPELNQSYTPYVVETSIGLDRTFLSIMATAYCEE
                     TLEGNDTRVVLKLPPALAPIKVCIMPLVKKDGLPEKAREIMDNLKFEFKCAYDEKDSI
                     GKRYRRQDAIGTPFCITIDHQTLEDNCVTIRHRDSMQQERVAIDSLETIVGKAVSMKK
                     MLQSIVQD"
     gene            10951..>11388
                     /locus_tag="PLJ82_04805"
     CDS             10951..>11388
                     /locus_tag="PLJ82_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR40858.1"
                     /translation="MSDEYTINENEDKELDNLAEAETEAFAASSGYKVPTVTTEATKH
                     HLTGMYQKWFLDYASYVILERAVPHLNDGFKPVQRRILHSMKLLDDGRYNKVANIVGH
                     TMQFHPHGDASIGDALVQLGQKDLLIDCQGNWGNILTGDGAAAP"
BASE COUNT         3222 a         2074 c         2062 g         4030 t
ORIGIN      
        1 tggtgtgaga ggctctcccc atcagtcttt actggtgggg cagtctactc gattaccagc
       61 cgtttttttg ctatctgtca caagttgatt tcttgcaagt tttgtagtcc cattttcaag
      121 tatttcgtat gagcacttgt tgtttttgcg ttataaactc ttgcaataat gtaattgtct
      181 ttacatctct gcataaaagc ccattcgctt ggtcttatgt aaattggaac agtatcgcct
      241 gtaacttcgc ctgttgtggt tgccttagca tcaatgaaat ataaaatctc gttgtttctg
      301 ctaagtatct caaaatcata ttctttgtat gtttcaccac ctttgttaag ccaattcacc
      361 ctactttctc caaactttgc aacaagttct ttatacacaa gttcttcgcc aaaacgtccc
      421 gtgcttccgt ctggaataat aggtggtggt gttggtggca tttcgtattt tgcaggaata
      481 ttacgaagca aaatactaac aaactcatct ttgcttattt ggctctctga aatatcaaaa
      541 gccttaaaca aatattcaat gagttttgca ttagaaatac tgttccaatt tccgctataa
      601 taaatcgtac tttgtgaagt atcatagtaa ttgggaacta tcggactgta attcagtttg
      661 ctacaactca ttttcaaatt tggactgcaa tagattttca aatttgaaaa cttgctttta
      721 agttggtttt caacactttg aaaatttatt gtatcaattt ttttcgcaat tcggcaatgc
      781 tgttgtttag tgttttgctt agttcaaccg catcatattt tgatgattgg gcatctaaac
      841 taaaatcaga aacagtaatt atttgtatgc caagttgctt taaaacgact tcaaattttt
      901 tccaataatc tttatcgcca aaccttttta caaccttatc actttcatct aagggcaaat
      961 aatttgccga tacgtcttgg taaaacaaac ttgttacact ttggaaggta tctttgttac
     1021 taagtaactt accattttgt ttaaaattgg caatgtctgt actgaatgaa cttaggcttt
     1081 catttgaaaa acgataaagt aaatcatcat agattagcct aatttttttg tcatcagtac
     1141 cgtttgaaac tgctatactg tttagaattt gaaaacactt agaaacagta agacgttgct
     1201 gtaaacctaa aaatgtttct aattctggac taaactcaat accacaagtt acagaactgt
     1261 catttaccaa atcctttaat ctatatgaat gcactatatt tggtgttctt actgcattat
     1321 ccaagcaagg aattgaagga tttgactgaa tgtaagtttt aaaagtgcta cttgtattga
     1381 ttgtaaaaag gctttgattt ttctgaaaat aagtccaaaa ctttttagca aaatcaaact
     1441 tgctcaaatg ttctttgtgc gaaacatcaa aataagaatt tcctacgttg cttctcctta
     1501 tctcttcatc aacacctatt tgtttaaaaa atgtttttag gtctgaataa gataaacccg
     1561 aaatagagtt aaaaatcggg cttatgaagt agtcataata acccgttaat aagtcttcta
     1621 tttctgtatt tgggctgtat tcttttggaa agtagcaaac agtaggcact gtcaaagaat
     1681 tgttttttaa caacaaagga attttcttta attcttcttt gtgtgttttt aataaatctt
     1741 ctttgaatga aagcaccgtt ttccaaactt gttgcacgtt ttgatgtgtt atcaaacttg
     1801 gatttgaggc taaaatgttt atttttcttt tgactatatc cgtagaagtg agttctacat
     1861 ttgaatttaa acgtagaaaa aactctttcc aattttggcg gttattgcta atctgttgta
     1921 aatagacact gtggatttga ttaggcaaaa cttgttcagg caaaacactt gctaaacgta
     1981 aatcttgtgt gtaatcatca ctcaaaatgc aagataaaat aggttgtaaa tgtccacttg
     2041 tagtaagcac ttggaaatta ctcaaattca catcaaaact tgaacggttt tgagtatcgt
     2101 taaaaacttc aaaaacaaac ttagttattt tcaaataatt atttgcgtcc atttttccgt
     2161 tttggataaa aggaactatt ttatctttaa aaatccgaac attttgagag tgttttagcc
     2221 ctgcaccata gaacaattta cgccactttg atttatcact tgcattttgg caataatccg
     2281 aactaatgaa attaaaatcg gttagcccta aatctgctgc aatttgctga acagagggct
     2341 ctccactatc tttgtaaaaa tcagataagt agcaagtatc caaattggaa actaatacat
     2401 tacttgtgtt gaaaacatag taaggtgcaa gtttttgaac ttcgctgtct aataactcgt
     2461 acttgaaaac aaaacgccaa aaactcaaat tattggtttt gtctgtaatt tttgaattga
     2521 tgtcgttaga aaaattgagt atgttttggc taataaaact tctaccttca aatgccttaa
     2581 cgcctaaact tgtaagccaa tttttgaact tagtatcact gtctgcaaag tcgttaatgg
     2641 ttgggtgtaa aaaataaact gccttaaagg taagcaaaga tttatctgcg tggtttgttt
     2701 gaaaatacat agatgaaggc gtaaacaaac cttgactact gttctcacta tacaataaac
     2761 ctatatcaga aaaattatat tctttttcct gtaaaaactt taaaacttct aaaagtaatt
     2821 gcggggttaa ggcttgttga aaactcgcat ttgcaaaaat tttttttagt tcatcttttc
     2881 caaaaatgct aattccttgc aagcctataa gttttgactt gaattctaat ctgttgtcaa
     2941 tgagtgtttt actcggattt acaactctta caaacaactc tttaccaatg atttcagaaa
     3001 gtcctgtcaa atctaccaaa gcatcagaaa ctttacataa agcaccgttt gcagtaggta
     3061 aaaacgcaat ttcctttatg gctttgtcaa aaccattatt gaaatattga tgtatgggtt
     3121 ctgcactatt ttgaaaacgt gaaggaacaa gattagttat tgtaaatctc aaatcttcat
     3181 tttgtgctat ttcactaatc catttgaaac ttaaaaaacc aatttgctca aaaaggtata
     3241 aattccaaat attttttacg tgaattgatt ggcggtttgc ggttgtcaaa aaatcggcat
     3301 ttaccaaaaa cggaaattta taatcattca cgttggttgg taaatacgta aataagaaag
     3361 acttatcagg gctgatactt tctattttgc tgtctttgat ttgacaagca aaacttagtt
     3421 tagacttctg tatttctttg agtttttcgg gtacaatttt gtcatttgcc attccttctc
     3481 ggatttctgg cgttactcca aactcaaaat cattgataat ccactcgtta tcatttaaga
     3541 caaaatagtt ttctccttta ctactttttt gtttttcaat aagaatagga ctttctaatc
     3601 ctaaaacttc tattgttttg atttttctca aaaaaagtga aaatcttggt tcattaaata
     3661 aggctttgat ttgactttta tagagtttga ttttttgaaa atcggtttct atggcaatag
     3721 ccacagggct atttttcata aaatcttcac actcttttat ttctcttgaa taacggtagt
     3781 tttcagccca gattggcttt aattgccaag gtatttcatc aatatttagc ccttcataaa
     3841 ggctactttg taactgatta taaaaagggt gttccttatc aaatgaaaag gaatacccac
     3901 ctgatttaat aagcacatta tcactttcac gaaaaacaac tttaaaacca atacctttgt
     3961 atcctgtctg ttcagggtct ttctttttgg tactgtatcc aatatctgtg attgccctta
     4021 tgtctgcttg gtcaaatggt tttccattat gaacgaaaag taaattttct tgtagaagaa
     4081 tgaacttaac attaacttca ttcttttcat ttggcatatc atcagcattt tgtaaaagtt
     4141 caaatacaaa tctttcggtt aagtctgtat ataaatctcc tgctaattta ctcaaagcat
     4201 tggcaatggt tcttgcatca tcaggtgttg caccacgttt tgaaaacaaa tctttgataa
     4261 acttttcttt ttcaagccta tcaaaaaagg tgcttttagg ttctcctaaa tgctttacaa
     4321 ggttaagcat aaaaggatta tccttgtctt ttctaaactt tacattagaa agtgatactt
     4381 ctaattgatg gaaaccactt ttattatttt gataatgttc tttatgcgga ctataaatac
     4441 gtacctgccc aagtcggaaa ggataatata aaatatctcc tttgggactt ctcacattta
     4501 cgaaaaatcc gtattcattt ccgtttttat caggtttttc agtcttttca taagtgccga
     4561 ttaaacggat agttccgttt tcggaaacac tacccatttc ccaccattgc aaaaggtagt
     4621 ctaaaatttc tttgtctgtc tttgcaaaga attgttttat ctgttcctga aagttcttca
     4681 ttgttgttgt gttgtttctt aaaatggcag gtaacatctt agtgttttat ttgtcgtttt
     4741 cgcataacaa agttaattaa aagcattaca ttttcaaata aaaacaaaat ttatttaatg
     4801 cattgacgaa aatggcaaat aaaacaacgt tgaacagtcc gattgctatg ggagaagtat
     4861 taatggtatg tctgctatgc taccatttgc atacggtcgc tacactatct gttatttaga
     4921 ttagtagatt tattgtaata catcattatc ccaatgtgca gggttatagg tcatgttttg
     4981 gttttttaat tcgcaaaccg tatccgtgtc ttttcccttt gctttgcgta atttcctttt
     5041 caatgggcag gctatcccta cctcacaggc tccaacttag cagttaatgg gtgtcggtgg
     5101 tgttctacgt ttaccaagtc catttttacg tagccgattt ttagtttggc ttctacgtag
     5161 gcaatgagtc tgtttttgtc gttagtaatc cagataaata cgcgttcgtt gtttttgaag
     5221 agggtgcctt cggctacttc tagttcaaat tttaggcagt cgtatctatc gccatttttg
     5281 agggtaacta tttctttttt ctcaaaacgc atttgcaagg gcgaagattt gccatcgcta
     5341 aaatagtcga tacgaaatgt ttggtttggt ttgagcgttt ctgcattgaa atcataattg
     5401 cgtaagcgat aaataatgct tactgcgtca tatgcacaat ttgttagcgt tgtgtttcct
     5461 gctttttcct tgtttttatt gtcagcaaat tgccagctta cccgcgttga gtcggtgtaa
     5521 tcatacttaa attctcgtgt ggctttaaat ttttcttcgt ttgcaatttc tttaaaataa
     5581 agcggttgta ggtcgtttac ttttaatttg gcatgtaagg tatcgcgtat agtaaaaaat
     5641 ttatccatat tgcccgctgt tacgcccgtt gtggtaatgg caaataccga ttctccttta
     5701 tatcgttctc tttttacttc gctttggatg ctaaaactgc tcaattgtag tattccccaa
     5761 cgaaaatagg cactatacct tagcttttcg cctgctttaa aacttgtatt agttatccca
     5821 cacgagtttg tttcttgtga gtgtatattc ccaataggca aaacgaatag cagtatatat
     5881 acgaatagtt tcttcatggc ttattttttg ctttttggta attcagtagc tcgttgttgc
     5941 aacaagggta tggcggtata atcccattct tctacactgt cccagttagc tcgcaatacc
     6001 aaacgcttag ggtagtaata cacttcttcg ggttctagct tgggcaatac gcttcccgta
     6061 tcccatttcc cgttttgatt agcatcgagt accaaacgca aaaaataatt gcctggacta
     6121 agaaggcgaa gtacggttgt tggttcgctt acgtgtactg cacgtacaac tgtttctttg
     6181 gcatctagca actccactat gccataaggt ggtatattct tccactcaat cattaacgaa
     6241 gcgtaatcat cgggcgattt ggttgtaaat gtttgtacaa acgaggtgtt ttgtttgcca
     6301 taatcactta ctatagctgc cgagtctatt accaagcgat agttcgctct ttcctttctg
     6361 tcgaacaata cactgatttt tttcttgcag ttatcgtcgt catattgcaa tgaaaaagga
     6421 acattgtgcc acgtagtatc tatacgcatg gtgagatgca ttttttcaat attaagcgat
     6481 ttcatcggtt cttttacctg cagacggaga gtgtctgcaa tatcgactgg cgattttgtt
     6541 agcgacacaa tttcaagctg ttgtagggtt ttctcgttag cagttcttgc tcgtggctgt
     6601 ttgtatgtta atgctattgt gtccgttttt aggctatctg cactggcata cgatactgta
     6661 aattggatag tatccagcgc taaggcgttg gagtcagtaa tccaatacac cagagagtcc
     6721 atgcggtttg tctgttcgcg tttcaaccac ttgctggttt ctacgttgat aggggtaatc
     6781 agcggttctt tgtcgagagc tgcggacata agtagagtaa acgactcttt gctggggcgg
     6841 agagctcttc gtaaagtagg aatcattgct cgctcctcgc gaaagagttt gataagcaaa
     6901 tcattgggtg catacgaaat agagtaagac gtgtcaattc gcaagataga aacagtatcg
     6961 ttgtcgttcc aaatagtatc tgtttgcagg tgttttacct cttcgggggt aactttttgg
     7021 ctgaaaaaac ctatttcttt tcctttttgg tcgtaataaa atgcttgtgg tatatctttt
     7081 agggcatata cttgataagg tacttgggga atgtttcgga ttgtaaaaaa cccttggcta
     7141 ttggtgcgag ccatataatc aaacggagta gaaacaaacg cactatcctt attgctttta
     7201 tataccccta caatgatgcc ttcgtatgga gtaagcgtgc gtgcatctat tacataacca
     7261 gcgagttgca tggtatcaat ggttgctcct gtggcaaaac tccacgagaa gttggcaagt
     7321 gcattttttt cgttcaaatc gaccacagca ttcccaaaat caatggtata ggtagtgttc
     7381 gaagcaagac tatccgcgaa agcaacgctc agttttttac ccattgctcg tatcgttggg
     7441 gcttgttttt gtggaggtga aacaaggata ttttcttgtt gtttttcaag ttgaatattt
     7501 tcgtcaaaaa tccattctaa tcgttggttt gttgctgaag tagctttgtt tgactggcta
     7561 ctttttagca attgtggggg tgttgtgtcg cgtatgccac cttgtggacc agcacctcta
     7621 ttggcacacg aatgcaatag caaggttaca cttacggtga tggctgaaat gagtaaaatg
     7681 cgtttaatca gggttgatat catggttgta aacttaattg taataattta tgtccaaatc
     7741 gacaatacaa acattttttt gtctgacaat actgtttttg tagctgtata agagcttgtg
     7801 tttgataggc tgaagtggct gtaaaaccca tttcttgcca atttcgcaca atactattgt
     7861 cttctgcagc acaactttcg agcaaatgta cggatttttc ttgtaattca tcattatttt
     7921 tcctttttgc atacgcaaat aatatgggga caatggtgtt gatgatgatg ctgttgatgg
     7981 ctttttctcc tagctttttt gtttttcgtg gcgaaacttc gccaaaggta taatgtgttt
     8041 cccaatacgg tgatgtgctt accgcaaaca tcttttttag gctgctataa gtagggtttt
     8101 ctactatgtg cgaaaaaagt tttgacgatg tgtgtatcaa ttgtgcaagt tgagccaacc
     8161 gaatgtgcgg aaaattagct ggtcgcaatc gcatcatctt aaataaactg ccatcgatag
     8221 gtgtaagcga aaatttttgt tgcaaaaagg catattcatt cttcaattgc acagtataat
     8281 catctgtggc ttggcttaac tgtaataaac ccgattgtcc aaaaagcaag gcttctattt
     8341 gtaaaagttg gtttttgtgt tttgctatgt agtttattgg taacgattta gctaaccatt
     8401 caaatgggtc gctgtttaat ccaaatccaa aattgcgtgc aagtgtttga taaaaagtct
     8461 cttcccaatt gtttttgttt tgttctagca aggtgtcaat gcgtgatgct ttggtttcca
     8521 aacgctctat aacgagagcc tctagccaac tagtttttat catatcactg atgcgatgta
     8581 ggtgcaaatg acaggctatc ttgtgcggac tgtgttccag ttgttgccat tgctccaaca
     8641 gagcaggggt aagtggcagt tttagttggg gtatttttct cccttcttgt gtaaaaatat
     8701 ctgtattaca ttctgcaact acatgcagta taacagaatt atatgctgga tttgttgggt
     8761 gtttgtgtgt attccaatcg gacgcttttt tatgaatttc tacattgccc gcccaaaggg
     8821 tatcgccaat cttaattttt gcgttgacaa aatccggacc agcatccaag ttgtgatgcc
     8881 ctacatcaat tatatctatc gttgaccctt ctgtgtcggt aagttgatgg ctagcaaata
     8941 accgatattt ccagatatga tgcaaaagtt cttccttcat gtgtttgctt ctttatcgtt
     9001 gtttgctctc attcacgacc agattaattg gtaatcggat agggtataaa aaaacctcct
     9061 ttatgtgtta gacaaaaagg aggtagtaaa ggtttaatct tgaacgattg attgtagcat
     9121 ttttttcatg cttacagctt tgccaacaat agtttctagg ctatcaatgg caacacgttc
     9181 ttgttgcatg ctatcgcgat gacgtatggt tacacagttg tcttccaaag tttggtggtc
     9241 gatggtaatg cagaaaggtg taccaatggc atcttgacga cgataacgtt taccaataga
     9301 atctttttcg tcgtacgcac atttgaactc aaatttcaaa ttgtccataa tttcacgtgc
     9361 tttttcaggc agaccatctt tttttaccaa aggcataata caaaccttga taggagctaa
     9421 ggctggcggt aattttagta ccacacgcgt atcattgcct tctaaggttt cttcgcaata
     9481 agcagtagcc attatcgaaa ggaaggttct atctaatcct attgaggttt caaccacata
     9541 aggcgtgtac gattggttaa gctcaggatc gaaatacttg atgtttttac ccgaatattt
     9601 ttcgtggcta cctaagtcaa aatctgtacg agagtgtatg ccttcaattt ctttgaaacc
     9661 gaatggtaat tcaaattcaa tatcagtagc agcattggcg taatgtgcta acttgtcgtg
     9721 gtcgtgaaaa cggtatttat ggtcgcccaa tccaagggct ttgtgccaac gcaaacggaa
     9781 ttgtttccaa tgttcgaacc atttcatttc ctcacctgga cgcacaaaaa attgcatttc
     9841 catttgttca aattcacgca tgcggaaaat aaactgacga gctacaattt cgttgcggaa
     9901 tgctttacct atttgagcta taccaaaagg aattttcatt ctgcccgttt tttgcacgtt
     9961 taggtagttt acaaaaatac cttgtgctgt ttcggggcgt aaatatattt tcattgcacc
    10021 atctgccgtc gaacccattt cggtgctaaa catcaagtta aactgacgca catcagtcca
    10081 gtttttagtg ccactaatag ggcaaacaac atcgtaatca acaataattt gtttcaattc
    10141 gttgaggtcg ccatcgttca aggcttttgc aaaacgagta tgaatttctt ctttttttgc
    10201 tttattttct tgtatgcgag gattggtttc ggcaaacaag gtagcatcaa acgcatcgcc
    10261 aaatttcttg gcagcttttt caatttcttt gttgatttta tcgtcatatt tcgccatcaa
    10321 atcttcgatt aacacatctg cacgatagcg ttttttcgag tctttgttgt ctattaatgg
    10381 atcgttaaaa gcatctacgt gtcccgaagc tttccatgtg gttgggtgca taaaaatagc
    10441 ggcatcaata cccactacgt tttcgtgtag caacaccatg ctatcccacc agtatttttt
    10501 tatgttgttt tttaactcta cgccgttttg accatagtcg tacacagcac ttaagccatc
    10561 ataaatttcg ctcgatggaa atacaaaacc atactctttg caatgtgcaa ttaatttctt
    10621 aaaaacatct tcttgtgacg ccatactata aaattagttc aattaaacga tattttttct
    10681 atctattgaa aatgagagac aaaactactg cttttttttg aattttggaa agcatctggc
    10741 taaattatcg tttatttgca ttgcttaacc ttgttttatg gaatcaaaaa acaggaaaaa
    10801 caactttttg acaaaaaatg ttcaaacttc gtcttttttt taattcattg gtatcaaaaa
    10861 aaattgtaat tttacagcca atctttttgc ggactcattt tttatgtttc taaaattgac
    10921 tatcgcattt ttctgcacca ttatttacgt atgagcgacg aatataccat aaacgaaaac
    10981 gaagacaagg agttagataa cctagcagaa gcagaaaccg aagcttttgc agcatcttct
    11041 ggctataaag tacctactgt tactactgag gctaccaagc atcatctaac gggcatgtac
    11101 caaaaatggt ttttggatta tgcttcttac gttattctcg aacgtgctgt accgcattta
    11161 aatgatggct ttaaacctgt tcaacgccgt attttgcact caatgaaatt gctcgacgat
    11221 gggcgttaca acaaagtagc aaacattgtg gggcatacca tgcagtttca ccctcacggg
    11281 gatgcttcta ttggcgatgc gctggtgcag ctgggtcaga aggatctgct tatcgactgc
    11341 cagggaaact ggggtaatat cctcaccggc gatggtgcgg ccgctccc
//
LOCUS       DAOSRP010000057        11279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5683_length_11279_cov_4.308624, whole genome shotgun sequence.
ACCESSION   DAOSRP010000057 DAOSRP010000000
VERSION     DAOSRP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5683_length_11279_cov_4.308624"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLJ82_04810"
     CDS             <1..960
                     /locus_tag="PLJ82_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40859.1"
                     /translation="KLVDAFLSGNVPASLLLFNEILNKGFDAHNFIVGLSEHLRDVLV
                     CKDERTAILLEVGASIRESYIQQAKACTDAFLYEALSLSNACDLEYKQSKNKRLLIEL
                     CLIKLCQILTPKVAQQQGSTPLQVIQTNTTQPQATPIQKEASKLVATPTYTTPLTKST
                     PPSTPISTSSVGSVSIKQISSSVSQQTQTIQPAKERTLREEPFTQVQLEEVWRKFGNE
                     IPDRHKMLFLFSSKKPKQIAKSSIELEVNDPLLVKDIEDIKPQLMAYLTQQLQNDKIS
                     LLITLVPLTAEHKAISNVDKIRLLKKKRPEFENMIQQLGLDPE"
     gene            975..1667
                     /gene="queC"
                     /locus_tag="PLJ82_04815"
     CDS             975..1667
                     /gene="queC"
                     /locus_tag="PLJ82_04815"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOR40860.1"
                     /translation="MVASTNKAYILLSGGQDSFVCLIWALQHFSEVEAVSIAYNQRHA
                     KEIEYAKKIAAHFNVPHFTYDIGSFMSSIAESSLLNTTNHNTQHAQASHLPASFVPNR
                     NGLFLTIIANHAFKQETKHIHLVTGTCETDYSGYPDCRDNYIKAKATELSLGLDISVS
                     IHTPLMWKTKAETFAMAHEAGKLNELIQLTLTCYNGEETMHPWGYGCGECPSCVLRKK
                     GYEEFLHLTYTV"
     gene            1674..2378
                     /locus_tag="PLJ82_04820"
     CDS             1674..2378
                     /locus_tag="PLJ82_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOR40861.1"
                     /translation="MKTSPVYYVAEIFESIQGEGNYAGVYSLFIRFQHCNLACSWCDS
                     KYTWYKNDTLIPQTADELKDIIASSTATHIIFTGGEPTLYALDKLVIPGKKYHVETNG
                     TVIPTEPLHIELPDGTYIERVAMKESIIKRFNWVVSPKLSNAINSQNTEYLHYWSARN
                     WGIFKFIIQQSADIDEIEHVINRYSIDKNRVYLAVEGCTLQSQLRPELVDELITKGFH
                     YSPRLHVLLWGAKRKK"
     gene            2389..2811
                     /locus_tag="PLJ82_04825"
     CDS             2389..2811
                     /locus_tag="PLJ82_04825"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOR40862.1"
                     /translation="MQYVITKEFTFEAAHRLLRNYCGKCSNNHGHSYRVKLHLQGTNL
                     DEKDMLIDFNETKKLKAWIDDNLDHVTMLWEEDPMIDSMRTFGNKVLVTKKNPTAEHI
                     AELILAEAQRLFENSAIQVKCIEINETCTSAARLYPLS"
     gene            2824..3204
                     /gene="queF"
                     /locus_tag="PLJ82_04830"
     CDS             2824..3204
                     /gene="queF"
                     /locus_tag="PLJ82_04830"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOR40863.1"
                     /translation="MNTPQFTHLGKKSNTPTKQLDTFPTPKNVEIVKFESDELTSFCP
                     VTHQPDFNRVIIEFSPDAYCIESKSLKLYLWSFREEAKFAEALAGEIADDIFNAIQPH
                     WCTITLIQNIRGGMQLSVVAKREK"
     gene            complement(3212..4648)
                     /gene="pepD"
                     /locus_tag="PLJ82_04835"
     CDS             complement(3212..4648)
                     /gene="pepD"
                     /locus_tag="PLJ82_04835"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOR40864.1"
                     /translation="MIFSLDPINVWEQFANLSSIPRASKNEEAIRLYLFDFAKKYSLL
                     AQQDATGNVLITKKTSQLDSQRPTIALQSHMDMVCEKEVTKQHDFTKDPITLLRDADW
                     IKADGTTLGADNGIGMALQLAVLMCDEDFGRDIECLFTVDEETGLTGAFGLEKDFLSA
                     PLMINLDSEQDDEIFIGCAGGCDTIAHFSIPKETLPSGYFFFEVSIHGLQGGHSGDDI
                     EKKRANANQLLNTYLLSIADKYSLWLGDVRGGSLRNAIPREAYAWCAVPRKFKESVRV
                     DWNIFIADVEQEWLAYEPSMRFVLSTTDAFAEGWSKDALADFLQAVSACPHGVINWSE
                     KMPTVVQTSTNLAVISSAHNGITVATNQRSLSEQERDEIAVRIRQLFELCGAEVLNQN
                     HYPGWEPRFEAAFLKHATDVYTTLFGQEPKVRVIHAGLECGVISGTYPAMDIISIGPT
                     IKDAHSPNERVSISSVQKIWEYLLALLR"
     gene            4826..5011
                     /locus_tag="PLJ82_04840"
     CDS             4826..5011
                     /locus_tag="PLJ82_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40865.1"
                     /translation="MIKAFLIAVGILLIAIVLLAVKMLFTKKGKFPSLHINENVALRK
                     KGITCAHSQDKLEQKKK"
     gene            5070..5666
                     /locus_tag="PLJ82_04845"
     CDS             5070..5666
                     /locus_tag="PLJ82_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOR40866.1"
                     /translation="MKNYLLISNIVLTIAVIVLLILMIFPKKSTKSALETTPTDTRLE
                     ETLPIAYVNIDSLLLNYNLYKKLSEELLQQEEKSRGKVTQQAVSLQKEMAEFQKKVEN
                     QAFLNAERAQAEQSRLLKQQQELQDLDARLTKELMSKQQKMNEQLKASIDSIILEFNK
                     DNKYHLILSNTGNDNILYGNKAYNITNEILKLMNVEKE"
     gene            5747..6715
                     /gene="fmt"
                     /locus_tag="PLJ82_04850"
     CDS             5747..6715
                     /gene="fmt"
                     /locus_tag="PLJ82_04850"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOR40867.1"
                     /translation="MTKEELRIVYMGTPEFAVAPLQSLLANQYNVVGVVTMPDKPAGR
                     GHKLQASAVKQVAIEHNLPILQPEKLKDPFFLEALRSLNADLFIVVAFRMLPEVVWNM
                     PRMGTFNLHASLLPQYRGAAPINWALIRGEKETGVTSFFLTHEIDTGDILLQRKIAID
                     NTDNAGSLHDKLMQLGAETVVETVAYILSGNTSKTPQNELMNEQQIHLAPKIFKETCR
                     INWSLSCEEIKNFVRGLSPYPAAYTEGLDAKGAPFSAKILHVSTQVCTPEARIGTVST
                     DNKTTLKIACADGWVIIDTIQVAGKKAMQTEELLRGFHLLVPQQLT"
     gene            6712..7074
                     /locus_tag="PLJ82_04855"
     CDS             6712..7074
                     /locus_tag="PLJ82_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOR40868.1"
                     /translation="MKKYVYIALGTISLGLGIVGIFVPLLPTTPFLLLTAALYCHSSD
                     KLYNKLMNHKTLGPYIHDFRTHKSIPLKTKIISISLVWITIGYSIMCVVPLLFVKILL
                     AAIAIGVTIHILSFKTKK"
     gene            complement(7082..7738)
                     /locus_tag="PLJ82_04860"
     CDS             complement(7082..7738)
                     /locus_tag="PLJ82_04860"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOR40869.1"
                     /translation="MMTATIHSFLPHQMDAIVLADGAFPTHPDLVALLLTHERVICCD
                     GAAEKLMQMGRIPSCVIGDLDSISAITKAQLGQKLIHQSDQETNDLTKAVHYCVENKL
                     YNVVIMGATGKREDHTLGNISLLALYHSLLHQVVMISDYGIFSAIDKTTVFSSYEHQQ
                     VSLFSILPTCPISVAGLRYPIIQRPLEMWWQGTLNESLGTSFTVQLHDKGLCIVFRKW
                     "
     gene            complement(7735..8331)
                     /gene="pnuC"
                     /locus_tag="PLJ82_04865"
     CDS             complement(7735..8331)
                     /gene="pnuC"
                     /locus_tag="PLJ82_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOR40870.1"
                     /translation="MDYLQQHWLEWFGVITGLLFLYLEIKQYPIMWIVGIITSAVYIV
                     VYLQASFYAYGLLNVYYVAISVYGLWLWVRGDNRNKTETTSSQHYRHIKGRLAVFVAA
                     ITIILCVLIYWALVSFTDSVMPSAEAIATALSIVATWMLAKRFIEHWFVWIVVNIVSV
                     TMFAMQGLYPTALLFFCYVFLSSIGYLQWRKFSKYEVL"
     gene            complement(8352..10595)
                     /locus_tag="PLJ82_04870"
     CDS             complement(8352..10595)
                     /locus_tag="PLJ82_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40871.1"
                     /translation="MKKVFISFSGLLLSASVAFGQADTLRLIDLQEVQVQSTRASSQT
                     PMTFSTVTKSDLQQQNAGQDIPFLLSLTPSVVATSDAGNGVGYTGIRIRGTDANRINV
                     TANGVPINDSESHDVFWVNMPDFSSSLQDIQIQRGVGTSTNGAAAFGGSLNMKTENLA
                     IVPYAELHGSYGAFNTSKLTVKAGSGLLRDRWAFDARLSTIQSDGFIDRASTDLSSYF
                     VQGGYFGKYTVIKLLSFGGKEQTYHAWDGVPKDKLQSDRTYNPSGYMGDDVFGNPMYY
                     DNQTDNYQQTHYHLLLNHSFSSSLSLNLALHYTKGKGYYEEYITGIKLKEYGLSNYVF
                     ESETITKSDLVRQKHLDNDFGGAVFSLDYAVDKLSVSWGGGANYYNGDHFGKITWIKN
                     YAGDATFVPGQQYYASVGEKTDANTYLKANYAVTEAINLYGDVQYRYINYQTYGTSDR
                     WDWMNNQMQVLNVNEDFHFFNPKAGIFYAIDEQNSAYASVAVGHREPTRNNYKDAELT
                     ANPTFERLINYELGYSYKHATYAFGANAYYMDYKNQLVLTGKVNEIGEALTSNTPESY
                     RMGIELTAGVQITPWLQWNGNLTVSENIIKKYTEYVDLYDENWDWTSQVATYLGDTPI
                     SFSPNLIANSVFSINHNGWSAALQSSYVGKQYIDNSGSDERSLSAYFVNNARLGYTFT
                     LKGMKELGIGILCNNLLNEQYESNAWVYSYYQQPDMNNPYKDRYADFGYFPQAGRNFM
                     LNMVLKF"
     regulatory      complement(10710..10808)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10944..>11279
                     /locus_tag="PLJ82_04875"
     CDS             10944..>11279
                     /locus_tag="PLJ82_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOR40872.1"
                     /translation="MPHIIIADNQAISRFGIYQLLSKQAISYQSTECTNKEELVIALR
                     EHTQAVIILDYTLFNFLGIDDLLVLAERYPRAHWLLFSDELSPDFLQRALCSATNISV
                     VMKDASQEEL"
BASE COUNT         3838 a         2249 c         1937 g         3255 t
ORIGIN      
        1 aaattggtag atgctttttt atcaggaaat gtaccggcaa gtttgctctt atttaatgaa
       61 attttgaaca aaggttttga tgcacacaat tttattgtag gattatcgga acatttgcgt
      121 gatgtacttg tatgcaaaga tgagcgtaca gccatattgt tagaagtagg agctagcatt
      181 agggaatcgt atatacaaca agcaaaagca tgcacagatg cttttttata cgaagcttta
      241 tcgttgagca atgcctgtga tttagagtac aaacaaagca aaaataaacg tttattaata
      301 gaattatgtc ttattaaatt gtgtcagata ctcacaccca aagtagcaca acaacagggc
      361 agcacaccat tacaagttat tcaaacaaac acaacgcaac cgcaagcgac tcccatacaa
      421 aaggaagcta gcaagctggt tgccacaccc acttatacca cacctcttac caaaagtaca
      481 cctccatcaa cccctattag tacatcttct gttggtagtg tttctatcaa acagattagc
      541 tcatctgtga gtcaacaaac acaaaccata caacctgcaa aggaaagaac actgcgtgaa
      601 gaaccattta cacaagtgca attagaagaa gtttggcgaa aatttggtaa cgaaattcca
      661 gatagacata aaatgttgtt tttgttttca agcaagaagc caaaacaaat agcaaaatcg
      721 agtattgaat tggaagtgaa tgaccctctg ttagtaaaag atatcgaaga cattaagcct
      781 caattaatgg cgtatttgac tcaacaacta cagaatgaca agataagttt gctcataaca
      841 ttggtaccac tcactgcaga acacaaagcc ataagcaatg tggacaaaat acgtttattg
      901 aaaaaaaaac gtcccgagtt tgagaatatg atacagcaat taggactaga tcctgaataa
      961 ataaaaacac agttatggta gcatctacaa ataaagcgta tatattgctt tcgggcgggc
     1021 aagattcttt tgtttgctta atttgggctt tacaacattt cagtgaagtg gaagctgtta
     1081 gtattgcgta taaccaacgt catgcaaaag aaatagaata cgcaaaaaaa atagcagctc
     1141 attttaacgt acctcatttt acgtatgata ttggttcttt tatgagtagt attgccgaaa
     1201 gttctttatt aaatacaacg aaccacaata cccagcacgc acaagcgtcg cacttacctg
     1261 cgagttttgt acccaatcgc aatggattat ttttaacgat tatagccaat catgcgttca
     1321 agcaagaaac caaacacatt catttggtaa cgggcacctg cgaaacagat tattcgggtt
     1381 atcccgactg tcgagacaat tacatcaaag ctaaagcaac tgaattatca ttgggtttgg
     1441 atatttctgt ttcaatacat actccgctga tgtggaaaac aaaagctgaa acatttgcta
     1501 tggctcatga ggcaggaaaa ttgaacgaac ttatccagct cacacttacc tgttataatg
     1561 gagaagaaac gatgcaccca tggggatatg gatgtggtga gtgtccttcg tgtgtattac
     1621 gtaaaaaagg atacgaagaa tttctacatc taacatacac tgtttaaatg cacatgaaaa
     1681 ctagccctgt atattacgta gccgaaatct ttgaatcgat tcagggagaa ggaaattatg
     1741 caggagtgta tagcttgttt atccgttttc aacactgcaa tttggcatgt tcttggtgcg
     1801 attctaaata tacttggtat aaaaatgaca cactcatacc tcaaacagcc gatgaactaa
     1861 aagacataat tgcctcatca acagctacac acattatttt tacaggaggt gaacctacct
     1921 tgtatgcatt ggataagtta gtaatacctg gtaaaaaata ccatgtagaa acgaatggaa
     1981 cggttatccc cacagagcct ctgcatatcg aattgcccga tggcacatac atagagagag
     2041 tcgctatgaa agaatccatc atcaaacgct ttaattgggt agtttcgcca aaactaagca
     2101 atgcgattaa tagccaaaac acggaatatt tgcactattg gtcggctcgt aactggggca
     2161 tattcaaatt tatcatacag caatctgcag atattgacga aatagaacac gtaattaatc
     2221 gttattctat tgataaaaac cgcgtatatc tagcagtaga aggatgcacg ttgcaatcgc
     2281 aattgcgacc tgaattggtg gatgaattga taacaaaagg ttttcactat tcgccacgct
     2341 tacatgtact attgtgggga gccaaacgaa aaaaataact atacaactat gcaatacgtt
     2401 atcactaaag aatttacatt tgaagcagct catcgtttac tacgcaatta ttgtggcaag
     2461 tgtagcaata atcacggtca ttcgtatcgc gtaaaattgc acttacaagg tactaacttg
     2521 gatgaaaaag acatgcttat cgattttaac gaaacaaaaa aattaaaagc atggatagac
     2581 gacaatcttg accacgtaac catgctctgg gaagaagatc cgatgattga tagtatgcgt
     2641 acgtttggca acaaagtact agttacaaaa aagaatccta cggctgagca tattgccgaa
     2701 ctcattttag cagaagcaca acgactgttt gagaactctg ccatacaagt aaaatgtatt
     2761 gaaataaatg aaacctgtac atctgcggca cgtttatacc ctttatctta aaagaataac
     2821 accatgaata cacctcaatt tacacattta ggcaaaaaaa gtaacacacc taccaaacaa
     2881 ctcgatacgt ttccaacacc caaaaacgta gaaattgtaa aatttgaaag cgatgaatta
     2941 accagttttt gtcctgtaac acatcaacca gattttaatc gtgttatcat cgaattttct
     3001 cccgatgcct actgtatcga aagtaaaagt ttgaaactct atttgtggtc atttcgcgaa
     3061 gaagccaaat ttgccgaagc attagcaggc gaaattgcag acgatatttt caatgctata
     3121 caaccacatt ggtgtacaat tacactcatt cagaacatac gaggaggtat gcaattgtct
     3181 gtggttgcta aacgggagaa ataaagttct gttatctcaa caatgccagc aaatattccc
     3241 atattttttg aacagaagat attgataccc gttcgtttgg cgaatgagcg tctttaatcg
     3301 ttggtccaat agagatgata tccatagcag gatacgtacc tgatataaca ccacactcta
     3361 acccagcatg aataacacga accttgggtt cctgaccaaa caatgtggta tatacatccg
     3421 tagcatgttt taagaaagca gcttcaaaac gaggttccca gcccggataa tggttttggt
     3481 taagcacttc agctccacac aattcaaaca attgcctaat acgcactgca atttcatcac
     3541 gttcttgctc actcaaactg cgttgatttg tagcgactgt gatgccattg tgagcagatg
     3601 atataaccgc cagattagta gatgtttgca caacagtagg catcttttca ctccaattga
     3661 taacaccgtg cggacacgcc gatactgctt gcaaaaaatc agctagagca tccttgctcc
     3721 aaccctctgc aaaagcatcg gttgtagaga gaacaaaacg catagaaggc tcataagcta
     3781 accactcttg ctccacatcg gctataaaaa tattccaatc aacacgtaca ctttctttaa
     3841 atttgcgagg aacggcacac cacgcataag cttctcgggg aatagcattc cgcaacgaac
     3901 caccccttac atcacctagc cacaatgagt atttatctgc aatagaaagc aaataggtat
     3961 ttaatagctg atttgcattc gcccgttttt tttctatatc atcgccacta tgcccgcctt
     4021 gcaagccatg tatacttacc tcaaaaaaga aatagccact tggcaaggtc tcctttggga
     4081 tagaaaaatg ggcaatcgta tcgcaaccac cagcacatcc tataaaaatt tcatcgtctt
     4141 gttcagagtc caaattaatc atcaaaggtg ctgataaaaa atctttctct aagccaaaag
     4201 cccctgtcaa acccgtttcc tcatccacag taaacagaca ctcaatatct ctaccaaaat
     4261 cctcatcgca catcaaaacc gccaattgca aagccattcc aatgccatta tcagctccta
     4321 gcgttgtgcc atcggctttt atccaatcag catcacgcaa taatgtaatc gggtctttgg
     4381 taaaatcgtg ttgtttagtt acttcctttt cgcacaccat atccatatgc gattgcaatg
     4441 caatagttgg acgttgtgaa tctaactgtg aagttttttt tgttatcaac acattgccag
     4501 ttgcatcttg ttgtgcaagt agcgaatatt ttttggcaaa atcgaataaa taaagacgta
     4561 ttgcctcttc attttttgaa gcacgtggta tggaactcaa gtttgcaaat tgctcccata
     4621 cattgattgg gtctaaacta aatatcatta ttttacaaaa ttatatgtaa gaatagctgt
     4681 cataatggac tctgaacaaa ataaattgtt aaaaaaaaac aaaatgttca agcttttatt
     4741 attaaagtac aaaaatacag aaaagctttt acaattgtct gttttatgtt ttatatttgc
     4801 gtacaatttg tactaattta tagttatgat aaaagcattt ttgatagctg ttggaattct
     4861 gttgattgcc attgtattac tggcagtaaa aatgttattt accaaaaaag ggaaatttcc
     4921 gtctttgcat atcaacgaaa atgtagcctt acgaaaaaaa ggcattacgt gtgctcattc
     4981 tcaagacaaa ttagaacaaa aaaagaaata agcagttcac tatttactta aaacacataa
     5041 taaccttact aaattataac aattactcta tgaaaaatta tttactcatt tccaacattg
     5101 tcttaacaat tgcagttatt gtgctactta tactgatgat ttttccaaaa aaatcaacca
     5161 aatctgctct tgaaacaacc ccaacagata ccaggttaga agaaacactt cctattgcat
     5221 acgtaaatat cgactcatta ctattgaact ataacttgta caaaaaacta agtgaagaat
     5281 tattgcaaca agaagagaaa tcgcgtggta aagtaacaca acaagctgtt tctttacaaa
     5341 aagaaatggc agaatttcaa aagaaagtag aaaatcaagc attcttaaac gctgaaagag
     5401 cacaagctga acaatcacgt ttacttaaac aacaacaaga gctacaagat cttgatgcac
     5461 gtttaaccaa agaattgatg agtaaacaac aaaaaatgaa tgagcaatta aaagcgtcta
     5521 ttgattccat tattttagaa tttaataaag acaataagta tcatctaatt ttaagtaata
     5581 ctggcaatga taatatttta tatggaaata aagcatacaa tattaccaat gaaattttga
     5641 aattaatgaa tgttgaaaaa gaataaggta taatttacgt cgcatcaaag ctactcttat
     5701 tatcacaata agagtggctt ttttcattat ctactattta tacaatatga cgaaagaaga
     5761 attacgcatc gtatatatgg gaacaccaga atttgctgtt gctcctctac aaagcttgtt
     5821 ggctaatcag tataatgtgg taggagtcgt tactatgccc gacaaacctg ctggtagagg
     5881 acataagcta caagcatctg ccgtaaaaca agtagccatt gaacataatc ttcccatttt
     5941 gcaaccagag aaactaaaag acccattttt tttggaagca ctccgctctt tgaatgctga
     6001 tttatttatc gtagtggctt ttcgtatgtt accagaagtt gtatggaata tgcctcgaat
     6061 gggcactttt aacttacacg cctccttatt accacagtac cgtggagcag ctcccatcaa
     6121 ttgggctctg ataagaggag agaaagaaac tggcgttact agttttttct taacccacga
     6181 aattgataca ggagatatcc tgttgcaacg aaaaatagct atcgacaata ccgataatgc
     6241 gggttcgcta cacgataaat taatgcaact aggagctgaa acggtagtag aaacggtagc
     6301 ctatattcta tctggtaata cttcgaaaac gcctcaaaat gagttaatga acgaacaaca
     6361 gatacacctt gccccgaaaa tatttaaaga aacttgtcgc ataaattggt cattgtcgtg
     6421 cgaagaaata aaaaactttg tacgaggttt atctccgtac cctgcagcgt atacagaagg
     6481 cttggatgcg aaaggggctc ctttttcggc taaaatatta cacgttagca cacaagtttg
     6541 cacccctgaa gcacgtattg gaacggtaag tacggataac aaaacgacac tcaagattgc
     6601 gtgtgctgat ggttgggtaa tcattgacac cattcaagta gcagggaaaa aggcaatgca
     6661 aacagaagaa ctactacgtg gattccactt gcttgtacca caacaactta catgaaaaaa
     6721 tacgtatata ttgcattagg taccatctcg ttagggctag gcatagtggg catatttgta
     6781 cccttattgc ctactactcc ttttttgttg cttacagcag ctttgtattg ccatagttcg
     6841 gataagctat ataacaaatt aatgaatcat aaaaccttag gtccctatat tcatgatttt
     6901 cgcacacaca aaagtattcc attaaaaacg aaaatcatat ccatatcatt ggtttggatt
     6961 accattggct attcgattat gtgtgttgtt cctctacttt ttgtaaaaat attgttagct
     7021 gctatagcta ttggcgtaac aatacatatt ttgtctttca aaacaaaaaa ataagcattc
     7081 tttaccactt tctaaataca atacataaac ccttgtcgtg taactgaaca gtaaaagatg
     7141 taccaagact ttcgttgagt gtaccctgcc accacatttc gagcggtcgt tgtataattg
     7201 gatatcgtaa tcccgccaca ctgataggac aggtaggaag tatggaaaaa agggagactt
     7261 gctgatgctc ataactagaa aaaactgttg ttttatctat ggcggaaaat attccataat
     7321 cacttatcat cactacttgg tgtaacagcg aatggtacaa cgccaacaac gaaatattac
     7381 ccaaagtatg gtcttcacgc ttgcctgtag cacccataat aactacattg tatagtttat
     7441 tttccacaca ataatgtaca gctttggtta aatcattggt ttcttggtca gattgatgta
     7501 tcaacttttg ccctaattga gctttagtta ttgctgaaat actatccaaa tcgcctatta
     7561 cacaagaggg aattctaccc atttgcatca atttttctgc tgctccatca caacaaataa
     7621 ccctttcgtg ggttaacaac aaagctacca aatcaggatg tgtagggaat gcaccatcgg
     7681 ctaacacaat agcatccatc tgatgaggta aaaaagaatg aatggtcgct gtcatcataa
     7741 gacttcatat ttagaaaatt ttctccattg taaataccca attgaagata aaaacacata
     7801 acaaaagaac aataaagccg ttgggtacaa gccttgcata gcaaacatgg taacagaaac
     7861 tatatttacc actatccaca caaaccaatg ttcgataaat cgttttgcca gcatccatgt
     7921 agcgactata ctaagagcag tagcaatagc ttctgccgat ggcataaccg aatcggtaaa
     7981 ggataccaaa gcccaataaa taagaacaca taagatgata gttattgccg caacaaaaac
     8041 agctaatcgt ccttttatat gcctataatg ttgcgaagag gtagtttctg ttttattccg
     8101 attatcgcca cgtacccaca accatagtcc atacacactt attgcaacat aatacacatt
     8161 aagtaagcca taagcataaa aactcgcctg caaatatacc acaatatata cagcagaagt
     8221 tataattccc actatccaca ttatcggata ctgctttatt tcgaggtata aaaagagtag
     8281 tccggtaata acaccgaacc actctaacca atgttgttgc aaataatcca tgtacaaagt
     8341 taatgatttt actaaaactt gagtaccata ttcaacataa agttacgccc tgcttgtgga
     8401 aaatagccga aatcagcata cctatctttg tatggattat tcatatctgg ttgttggtaa
     8461 tacgaataca cccaagcatt actttcgtat tgttcattca acagattatt acataaaata
     8521 ccaataccta attctttcat tccttttaag gtgaaagtgt aaccaagtcg tgcattgttt
     8581 acaaaatagg ctgataagga gcgttcatca ctacctgaat tgtcaatgta ttgcttgcct
     8641 acataacttg attgcaatgc tgctgaccat ccgttatggt tgatactaaa tacactattg
     8701 gcaattagat taggtgaaaa tgaaataggg gtatcgccca aataagtagc aacttgcgat
     8761 gtccaatccc aattttcatc atacaaatct acgtattcag tatacttttt gatgatattt
     8821 tcacttacag ttaggttacc gttccattgt agccaaggag taatttgcac acccgcagta
     8881 agttctattc ccatacggta actctctggc gtattgcttg ttaaggcttc tcctatttcg
     8941 tttaccttac ccgttagaac caactgattt ttgtaatcca tgtaataggc attggctcca
     9001 aaagcatacg tagcatgttt gtaagaatac cctagctcat aattaatcaa gcgttcaaaa
     9061 gttggattgg ctgttaactc agcatctttg tagttgtttc gagtaggctc tctatgccct
     9121 acagccaccg atgcataagc agaattttgc tcgtcaatgg cataaaatat acctgctttt
     9181 ggattaaaga aatgaaaatc ttcgtttaca ttcaaaacct gcatctggtt attcatccaa
     9241 tcccaacgat cgctagttcc gtatgtttgg taattgatat agcgatactg tacatctcca
     9301 tacaaattga tagcttctgt tacagcataa tttgctttta aataagtatt tgcatctgtt
     9361 ttctccccta cgctagcata atattgttgc cctggcacaa atgtagcatc gccagcataa
     9421 ttttttatcc atgttatttt tccgaaatgg tcgccattat aataattagc tcctccaccc
     9481 catgacacag acaatttatc cacagcataa tcgagcgaaa atacagctcc accaaaatca
     9541 ttatccaagt gtttttgacg cactaaatca cttttagtaa ttgtttcact ctcaaacaca
     9601 taatttgaca agccatactc tttcaatttt atacctgtta tatattcctc ataataccct
     9661 tttcctttgg tatagtgcaa tgccaaattg agcgataagg aagaactaaa agaatgattt
     9721 aacagcaagt gataatgcgt ttgttggtaa ttgtctgttt gattgtcata atacatcgga
     9781 ttaccaaata catcatcgcc catatagcca cttgggttgt aggtacggtc tgattgtagc
     9841 ttatctttcg gcacaccatc ccacgcgtga taggtttgtt ctttgccacc aaacgacaac
     9901 agtttgatta ccgtgtattt accgaaatat cccccttgta caaaataaga agataaatcc
     9961 gtagatgccc tatcaataaa accatccgat tgtatggtag ataaacgagc atcgaacgcc
    10021 caacggtcgc gtaataaacc cgaacccgct tttacggtaa gtttagaagt attaaacgca
    10081 ccataacttc catgcagttc agcatacgga acaatggcaa gattctctgt tttcatattc
    10141 agactaccac caaaagcggc tgcaccattg gtagaagtac caacgcctcg ctgaatctga
    10201 atatcttgta atgatgagga aaaatcgggc atgtttaccc aaaatacatc atgcgattct
    10261 gaatcgttaa tcggtacgcc attggcagta acattgatac gattagcatc tgtaccacgt
    10321 atgcgaatac ccgtataacc taccccattg cccgcatccg aagtggctac gaccgagggt
    10381 gtaagcgaca ataaaaacgg aatatcttgt cctgcatttt gttgttgcaa gtcagacttg
    10441 gtaaccgtag agaatgtcat tggtgtttga ctcgaagcac gagtagattg cacttgtact
    10501 tcctgtaaat caattaaacg aagcgtatct gcctgcccaa aggctacaga tgcagacaat
    10561 aaaagtccgc tgaaagaaat aaataccttt ttcatttaga aacattttta ctttttgaaa
    10621 tgccataacg taaacgccaa gagccatttc aaaaaaaaga ttaaacaaaa aaggggtaat
    10681 aaaaagagaa gaataccgaa aagcttgtgc acaaagcgaa tctttttccc tgcgatggca
    10741 ttatccatat caggttcaaa gggtataatc tcagcctgag aaaaactcaa gcaccccata
    10801 catatcgatg caaaagtaca cattattttt agaataattg aaatatgaaa gaaaaattat
    10861 gttttataac atatcagaag gtttgttaaa agtataatca ataaaaaaaa agtgtatctt
    10921 tgttataaga aaacatagca actatgccac acatcatcat agccgataac caagccatta
    10981 gtcgttttgg catctaccaa ctgctaagca aacaagctat atcctaccaa tctaccgaat
    11041 gtacgaacaa ggaggaattg gtaattgcac ttagagaaca tacacaagcg gtaattattt
    11101 tggattatac cctatttaat tttttaggca ttgacgattt attggtatta gccgaacgat
    11161 atccacgtgc tcattggtta ttgttttcag atgagttgag tcccgacttt ttgcaacgag
    11221 ccttatgtag tgcaaccaat attagtgtag taatgaaaga tgcttcgcaa gaagagtta
//
LOCUS       DAOSRP010000058        10981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5855_length_10981_cov_5.352004, whole genome shotgun sequence.
ACCESSION   DAOSRP010000058 DAOSRP010000000
VERSION     DAOSRP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_10981_cov_5.352004"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLJ82_04880"
     CDS             complement(<1..371)
                     /locus_tag="PLJ82_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOR40873.1"
                     /translation="MAKEIKFNIEARDQLKRGVDELANAVKVTLGPKGRNVIISKKFG
                     APHITKDGVTVAKEIELEDAFENMGAQMVKEVASKTGDDAGDGTTTATVLAQSIITVG
                     LKNVAAGANPMDLKRGIDKAVA"
     gene            complement(425..694)
                     /locus_tag="PLJ82_04885"
     CDS             complement(425..694)
                     /locus_tag="PLJ82_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOR40874.1"
                     /translation="MNIKPLADRVLIKPTAPEEKTASGIIIPDSAKEKPLKGSVVAVG
                     NGTKDEEMVVKTGENVLYGKYAGTEIDFDGEKYLIMRQSDILAII"
     gene            complement(852..1193)
                     /gene="secG"
                     /locus_tag="PLJ82_04890"
     CDS             complement(852..1193)
                     /gene="secG"
                     /locus_tag="PLJ82_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOR40875.1"
                     /translation="MYTAIVTLIVIVSVLLTLVVLAQNSKGGGLASNFSGSNQIMGVR
                     KTTDFIEKATWTLASVLLFLCIISVAFIKQGTADETSTSAIINEAEAATKTNSDVQLP
                     TFPIENPETQE"
     gene            complement(1198..1854)
                     /locus_tag="PLJ82_04895"
     CDS             complement(1198..1854)
                     /locus_tag="PLJ82_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40876.1"
                     /translation="MTGQDILFYMEHPDKLGNHTLADMKEIANKYPFFHGIHQLLLLN
                     MHHTNDMLYASTLKKSILYINDKQTFKKRLQQPVSNITVNPLLSVASTNETSTTVPLT
                     SEPALETYRLDDNKASSNIRLKNQDLIDNFIELNPKIKPIGANESETIIIPVEHNDEE
                     NEEFFTETLAKIYIKQKQYEKAINIFKRLNLKYPEKSVYFADQIRFLEKIIHLNSKNT
                     "
     gene            complement(1859..2386)
                     /locus_tag="PLJ82_04900"
     CDS             complement(1859..2386)
                     /locus_tag="PLJ82_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOR40877.1"
                     /translation="MKKTRFTSIGYLIIPLLIAVMGVSCSVSYKFSGTSIDYSRTKTI
                     YVRDFTNQAQMVYAPLTDVLNETVKDIYARQTRLSLVNRNGDLEIEGEITNYDISSMS
                     IQADAIAAETRLTIRVKVRFTNNKNHDEDFERTYTSFRNFSSNLMLNDVQDELITEMT
                     KEIAELIFNDTVANW"
     gene            complement(2410..3630)
                     /locus_tag="PLJ82_04905"
     CDS             complement(2410..3630)
                     /locus_tag="PLJ82_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOR40878.1"
                     /translation="MDEKELQAIKQRFQIIGNTPALNRAIEIATQVAGTDLSVLITGE
                     SGVGKESFPKIIHQFSPRKHEKYIAVNCGAIPEGTIDSELFGHEKGSFTGALNDRKGY
                     FEVADGGTIFLDEVGELPLPTQARLLRVLEVGEFIKVGSSNVLKTNVRVVAATNLNIP
                     KAIQEGKFREDLYYRLNAVQIAVPPLRERKNDIPLLFKKFASDFAEKYRMPTIYLTPE
                     AQEVLVDYYWQGNIRQLKNIVEQLSIIEEHREITSDCLQNYLPKPTKEQLPLLIANTH
                     NNEKSFSNEREILYQVLFDMKKDIDELKKMVHQYKQPPVTPTKNTDNEPSFLPSIVDK
                     NTLPKTEKHIKKVEIEDAQEYEEHNFSVNEMEKNLIKKALEKHNGKRKLAALDLKISE
                     RTLYRKIKEYDLDA"
     gene            complement(3667..4671)
                     /gene="pdxA"
                     /locus_tag="PLJ82_04910"
     CDS             complement(3667..4671)
                     /gene="pdxA"
                     /locus_tag="PLJ82_04910"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOR40879.1"
                     /translation="METKKLKIGITQGDINGIGYEIILKTLDDARILEFCTPILYGSP
                     KVAAYHRKNLEITLNLNLITNAAEANEKRVNIINCNDDEIKVELARSTDEAGKAAYLA
                     LEKATQDLKSGAIDALVTAPINKKNIQSDEFTFPGHTEYLEEKFGNKGEALMLMVNDF
                     MRIAVVTGHIPITKVAQAITTEKIVEKIHQLNNALKQDFMLTKPRIAVLGLNPHAGDD
                     GVIGDEEKTIILPAIEKALQEGIICVGPLPADGFFGASNFHHYDGILAMYHDQGLIPF
                     KALSMDSGVNYTAGLSVVRTSPAHGTAYDKAGQNLANESSFREALYLACDIVNNRQRS
                     "
     gene            complement(4697..5677)
                     /locus_tag="PLJ82_04915"
     CDS             complement(4697..5677)
                     /locus_tag="PLJ82_04915"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOR40880.1"
                     /translation="MKNVVLLILTSLLLLTGCDEKSLKPTITGSAFEILLVVDEPIAA
                     SEAGKTLFNILNQDMYGLPQSEPLFKVSRTPHSGFTAILRPARNIIQLEINDTYSRTR
                     IVTQNDVWAAPQAVIKITSNSVEAAQQAIEKYQQDILDFFVTAERDRTIAYQKRYAEK
                     DAMKKVQDKFGISIVLPKGMNRFKEEENFLWISNASDDVRQNIVIYTYPYTSKETFTR
                     ENLLHKRDSVMLLWVPGPNPDSYMATEYRVDPPILNEIAINGTYAAELRGMWRVQGDL
                     MGGPFVNITRLDEKNQRVVTVEAFIYAPTKNKRNAIRQVEALLHTVEFTN"
     gene            complement(5687..6715)
                     /gene="rlmN"
                     /locus_tag="PLJ82_04920"
     CDS             complement(5687..6715)
                     /gene="rlmN"
                     /locus_tag="PLJ82_04920"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOR40881.1"
                     /translation="MIKKALFGKNLSEITEIVSANGLPAFAAKQITDWLYKKEISDIE
                     EMSNISAANRKKLADNYCVGIEKPVQVSVSSDGTKKYVFQVGNKHFIESVFIPDKERN
                     TLCISSQIGCKMNCLFCATGKQGFSGNLTANQILNQMRSIEEFNSLTNIVFMGMGEPL
                     DNITEVLKAIEIITSSYGYGWSPKRITVSTVGVLAPLKQFLEKSQCNLAISVHAGNSF
                     VRKELVPLEKTQPMQEIMALVKNYDFTHQRRLSIEYILFDQLNDTEEQALLLASMLKQ
                     IPCRINLIPYHRIPQVGLMPSSREKIENFQKILMKKGITTTIRRSRGQDIEAACGMLS
                     TKFVNTSN"
     gene            complement(6877..9681)
                     /locus_tag="PLJ82_04925"
     CDS             complement(6877..9681)
                     /locus_tag="PLJ82_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOR40882.1"
                     /translation="MKKLIASFFLLLFAVICLAQQQQLPIIPTDPQVLIGQLPNGMTY
                     YIRKNSLPKERADFYIAQKVGSVIEEPNERGLAHFLEHMAFNGTTHFPNKDLLNYLQR
                     IGVKFGENVNAYTSVEQTVYMVTNVPVVRETVIDSCLLILRDWANGITLSDSEIEKER
                     GVIEEEWRSRNSAWLRMAETILPTIYGNDKYADCLPIGHIDVIRNFKPDELRAFYKKW
                     YRPDLQAIIIVGDIDPQQVEAKLKATLGTIAMPSNPIPRPVYAVTDNQEPIIAFARDK
                     EATAISVEVSIKQDVFPTEMKGTIAYMMQEYVHAAVSGMMGSRFNEMIQKPNPPFTQA
                     YAYTDNFWVSNTKDARTLYAIASEQGATTAIKSLITENERMRQFGFTIAEYEREKANI
                     LKNIENQYNEREKQQNYEYVPKYINHFLENTPIPSIEYTFETYKQLAEMIPLELINNI
                     VKQASTTGNMVIVVQGPEDEKISFPTAEEILSGIEEVKRATIEPYTENVSNEPLISKL
                     PKPGKIKKEITHSNGTTDFILSNNVTVRLVPTNHKDDEILFQAYSHGGYSLVDKQYAT
                     TLKVLPELSTLSGLGNFSAIDLQKRLSGKNLRLSKQLNTYSESLSGYSGKKDVEMLFQ
                     LAYLHFTGIRTDEDAFQAYINRQLAVLKNRENSPYMTFIDTINHVLYDSHPLMTRLKS
                     TDLSTIDYQKGMKILSERYANAADFSFVIVGNIDSETLRPLLTKYIATLPAKGKKETW
                     KDAGVTIKEGNNTLHYNKIMEVPKSSTIQYTTGKIPYTLENRIKLTILSQILDIVYTE
                     KVREDEGGTYGVSVDGNLDAIPYNRFTLETYFDTDESKRSKLVEIINQEFLAIASQGP
                     RTQDLQKVKEFLTKQYQENLIENWYWQNVEIEKIITNLDIHTDYLTILEKIDEHAIQQ
                     FANVIVQTSNKKEIVQIGTK"
     gene            complement(9698..9964)
                     /locus_tag="PLJ82_04930"
     CDS             complement(9698..9964)
                     /locus_tag="PLJ82_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40883.1"
                     /translation="MIVLLAASIFIIAENKLWIGWLLQLVGIIPWIYVFILNEYIYLL
                     PEYQYEVFLIPASFMLWCSFAICIISLLKITSILYTKHTNKQLQ"
     gene            complement(10137..>10981)
                     /locus_tag="PLJ82_04935"
     CDS             complement(10137..>10981)
                     /locus_tag="PLJ82_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40884.1"
                     /translation="LSYNNKLATYNQEGTLLQDTTLTYNGVLYQFIQEDSTFWVDIFN
                     GRQHLLQKFSNNNELLLDRKIDELSDILYVGNNKFIIPGYNKNSNTYRMELYDAYNDT
                     VINFINIGEITNLKDSITNAKGTITFTNKFATNKQGQVLAYYRYNSYFLLMDGNLEYT
                     AHQDFRKFPIGEPSYKGHNVLLNPLNCGIDKACIDDKFIYLLTPTYVCKQWNKAKKDR
                     MLDIYSLTTKAYLGSIRFPTIKNKEIVSIENTTKGFVTVYANDKETEIVTYNNDFLSH
                     FLYE"
BASE COUNT         3001 a         1924 c         2027 g         4029 t
ORIGIN      
        1 gctacagctt tgtcgatacc tctttttaag tccattgggt tggcaccagc agctacgttt
       61 ttaagaccta ctgtaataat tgattgagcc aatacagtag cagtagtagt accatcacca
      121 gcatcgtcgc ctgttttaga ggctacttct tttaccattt gtgcacccat attttcgaat
      181 gcatcttcca attcaatttc tttggctaca gttacaccat ctttggtaat gtgtggagca
      241 ccaaattttt ttgaaataat cacgttacga cctttaggtc ctaatgttac ttttactgca
      301 tttgctaatt catccacgcc tctttttagt tggtcgcgtg cttcgatatt gaatttaatt
      361 tcttttgcca tagttatact ttttttctac tgttattttt ttactctatt tcactaacac
      421 attgttaaat gattgctaaa atgtcacttt gacgcatgat aaggtatttt tcgccatcga
      481 aatcaatttc tgtaccagca tatttgccat acagtacatt ttcgccagtt tttactacca
      541 tttcttcgtc ttttgttccg ttacctacgg ctacaacact gcctttaagg ggtttttctt
      601 tggctgaatc aggaatgata atgccacttg ctgttttttc ttctggtgca gtaggtttaa
      661 ttaacactct gtctgctaat ggtttaatgt tcatagatgt catattatta ggttaaacat
      721 tttaatttac attgatagta tgtgcataat gtatgccaaa gtatttttct gtcatttttg
      781 tcagttttac tgacaaaaaa aagccgtttg gagtatgttc caaacggctg ttatgataag
      841 gatatctatt tttattcttg tgtttctgga ttttcgatag ggaaggttgg cagttgtaca
      901 tctgaatttg ttttggtagc agcttcagct tcgttaataa tagcagaagt ggatgtttca
      961 tcagcagttc cttgttttat aaaagccaca gaaataatac ataaaaaaag taaaacagaa
     1021 gccaatgtcc atgtcgcttt ttcaataaaa tcagttgttt tacgtacgcc cattatttgg
     1081 ttagatccag aaaaattaga agctaatccg cctccttttg agttttgagc taaaacaact
     1141 agtgttaaaa gcacggaaac aattactatt aatgttacga ttgctgtgta cattgtgtta
     1201 cgtatttttt gaattaagat gaataatttt ttctaaaaaa cgaatttggt ctgcaaagta
     1261 aacacttttt tctggatatt tcaaatttaa tcttttaaaa atattgattg ctttttcgta
     1321 ttgtttttgt ttgatataaa tttttgctaa tgtttcggta aaaaattctt cgttttcttc
     1381 atcattgtgc tctacgggga tgataattgt ttcggattcg ttagccccga taggtttaat
     1441 cttaggattt aattcgataa aattatctat caaatcttgg ttttttaaac ggatattgct
     1501 ggaggctttg ttgtcgtcta agcggtatgt ttcaagagcc ggttcgcttg tcagtggcac
     1561 agtggtagac gtctcgtttg tacttgctac ggatagtagc gggttaactg tgatattgga
     1621 tactggttgt tgcaaacgtt ttttaaatgt ttgtttgtcg ttgatgtata agattgattt
     1681 ttttagtgta ctagcataca acatatcgtt tgtgtgatgc atattgagta gcaacagttg
     1741 gtgtatgcca tgaaaaaagg gatatttatt ggcaatctct ttcatgtcag caagagtatg
     1801 gtttccgagt ttatcgggat gttccatata aaacagtata tcttgtccag tcatagttct
     1861 accaatttgc tacagtatcg ttaaaaatca gttctgctat ttcttttgtc atttcagtaa
     1921 ttaattcatc ttgcacatca tttagcatca ggttactact aaaattacgg aaagatgtgt
     1981 acgtgcgttc aaaatcttcg tcgtggtttt tattgttcgt aaaacgtact tttacgcgta
     2041 tggttagtcg tgtttcggca gcaatagcat ctgcctgaat agacatagac gaaatgtcgt
     2101 agtttgtaat ttctccctct atttctaagt ccccatttcg gttgactagc gaaaggcgtg
     2161 tttggcgagc atatatatct tttactgttt cgtttaatac atcagtaaga ggagcatata
     2221 ccatttgagc ctgattggta aaatcgcgta cgtaaattgt tttggtacga ctataatcaa
     2281 tagaagtgcc tgagaatttg tatgatacag agcaagaaac gcccattact gctataagta
     2341 atggaataat tagatagcca atggatgtaa aacgtgtttt tttcatggtt tgtgtgcgtt
     2401 aaaatctgac taagcgtcta aatcatattc tttaatttta cgatataagg tgcgttcaga
     2461 tatttttaaa tccaaggcag ccaatttgcg tttaccattg tgtttttcga gtgctttttt
     2521 tatcaagttt ttctccattt cgtttacact aaaattgtgt tcttcgtact cttgagcatc
     2581 ttcgatttct acttttttaa tgtgcttttc tgtttttggc aacgtatttt tatccacaat
     2641 tgatggtaaa aaagaaggtt cgttatccgt attttttgtc ggagttacag ggggttgttt
     2701 gtactgatgc accatttttt ttagttcatc aatgtccttt ttcatatcga ataacacttg
     2761 gtataagatt tctcgttcgt tagaaaacga tttttcgttg ttatgtgtgt ttgctataag
     2821 caacggtaac tgttctttcg tgggttttgg taaatagttt tgcaagcaat cgcttgtaat
     2881 ttctctgtgc tcttcaataa tggatagttg ctcaacgatg ttttttaatt gacgaatatt
     2941 cccttgccaa taataatcaa ccaatacttc ttgagcctca ggggtaaggt aaatagtagg
     3001 catacggtat ttttcggcaa agtcggatgc aaatttttta aataataagg ggatatcatt
     3061 ttttctttcg cgtagtgggg gtactgctat ttgtacagca ttgagtctgt aatacaaatc
     3121 ttcacgaaat ttaccttctt gtatggcttt ggggatgttc aaattagtgg cagctactac
     3181 acgcacgttt gtttttaata cattcgatga gcccaccttg ataaattcgc ccacttcgag
     3241 cacacgcaac aaacgagctt gggtaggcaa gggtagttcg cccacttcgt ctaaaaagat
     3301 tgtgccacca tcggctactt caaaataacc ttttcggtcg ttgagtgctc ctgtaaaaga
     3361 gcctttttcg tgtccaaaaa gttccgaatc aatcgttcct tcgggtatag caccacagtt
     3421 tacggcaatg tatttttcgt gtttacgagg actaaattgg tgtataattt tcggaaaact
     3481 ttcttttcct acaccgcttt cgcccgttat tagtacagat aagtctgtgc cagctacttg
     3541 agtagctatt tcaatagccc tattgagtgc tggcgtattg ccaataatct gaaaacgttg
     3601 ttttattgct tgtaattctt tttcgtcaat catactacaa tcctttccac taaagtgttg
     3661 aatagattag cttcgttgtc tgttgttaac tatatcacat gccaaatata atgcctctct
     3721 aaaagacgat tcgttggcaa gattttgccc tgctttgtcg taggcagttc catgagcagg
     3781 cgaggtgcgt actacggata atcctgctgt ataatttacg cccgaatcca ttgataaggc
     3841 tttgaaaggg attaaacctt ggtcgtgata catcgccaaa atgccatcgt aatgatgaaa
     3901 atttgatgca ccaaagaacc catcagcagg tagtggtcct acgcaaataa ttccttcttg
     3961 cagtgctttt tcaatagctg gcaaaatgat tgttttttct tcatctccta ttacgccatc
     4021 atcaccagca tgcggattta agcctaaaac agctatacgt ggtttggtta acatgaaatc
     4081 ttgtttcagg gcattgttga gttggtgaat cttttctaca atcttttcag tagttattgc
     4141 ttgagctact ttggtaatgg gaatatgacc tgtaacaaca gcaatacgca taaaatcatt
     4201 caccatcaac atgagtgctt cgcctttgtt gccaaatttt tcttctaaat attcggtatg
     4261 acccgggaaa gtaaattcat ctgattggat attcttttta ttgataggag ctgtaactag
     4321 ggcatctatt gcacctgatt tgaggtcttg agttgctttt tccagggcta agtaagctgc
     4381 tttgccagcc tcatccgtag agcgtgctag ttctactttt atttcatcat cgttgcagtt
     4441 gataatgttt actcgtttct catttgcctc agctgcattg gtaataaggt tgaggttaag
     4501 tgttatttct aagtttttgc ggtgataggc tgctactttg ggcgaaccgt atagtatagg
     4561 tgtgcaaaat tcaagaatgc gggcatcatc taatgttttc agaattattt cgtatcctat
     4621 tccattgata tcaccttgcg taatacctat ttttagtttt tttgtttcca tatcgattgt
     4681 ttttcagcta gtttttttag ttagtaaatt cgactgtgtg caacaatgct tctacttgtc
     4741 gaatggcgtt gcgtttattt tttgttggag cgtatataaa tgcttctacg gttactacac
     4801 gttgattttt ttcgtctaat cgggtaatat ttacaaacgg accacccatt aaatctccct
     4861 gtacacgcca cataccacgt agttcggctg cgtatgtacc gttaattgct atttcgttaa
     4921 gtatgggagg atctactcgg tattcggtag ccatatacga atctggattt ggtccaggca
     4981 cccacaagag catgacagaa tcgcgtttgt gtaacagatt ttcacgcgta aatgtttctt
     5041 tgctggtata agggtaggtg tagataacaa tattttgacg aacatcgtcg ctagcattgc
     5101 ttatccacaa aaagttttct tcttctttaa aacggttcat acctttgggt aatacgatgc
     5161 taatgccaaa tttatcttgc acttttttca tcgcatcttt ttcagcatag cgtttttggt
     5221 atgctatagt gcggtctctt tcagctgtta cgaaaaaatc gagaatgtct tgttggtatt
     5281 tctcaatggc ttgctgtgca gcttctaccg agttactcgt aatttttatt accgcttgcg
     5341 gagcagccca tacgtcgttt tgagttacaa tgcgtgtgcg tgaatacgta tcgtttattt
     5401 ccaattgaat gatgtttcga gctggacgca agatggcagt aaacccacta tgcggggtgc
     5461 gagacacctt aaataaaggt tcggattgag gcaagccata catatcttga ttgagaatat
     5521 taaaaagcgt ttttccagct tcgctagcag ctatcggttc gtctaccacc agcaaaattt
     5581 caaaagcact tcctgtaata gttggtttta ggcttttttc gtcgcatcct gtcagtaata
     5641 acagggaagt aagtataaga agaaccacgt ttttcatagg ctataattag tttgaagtat
     5701 tcacaaattt tgttgataac ataccgcaag cagcctcaat gtcttgtcca cgtgagcgtc
     5761 ttatagtggt agtaatgcct tttttcatca gtattttttg aaaattttcg attttttcgc
     5821 gactagaagg cattaatcca acctgtggaa tacgatgata tggaataaga ttgattctgc
     5881 acggaatttg tttaagcata cttgctaata acagagcttg ctcttcggta tcgtttagtt
     5941 gatcgaacaa aatatattcg attgacaaac gtctttggtg tgtaaaatca taatttttta
     6001 ccaaagccat tatttcttgc atgggttgtg ttttttcgag tggcaccaat tccttgcgaa
     6061 caaaactatt acctgcatgt acgctgatag ccaagttaca ttgcgacttt tctaaaaatt
     6121 gtttaagagg agctagtacc cctacggtag atacggtaat gcgttttgga ctccaaccgt
     6181 aaccatagct agacgtaata atttctattg cttttaatac ttcggtaata ttgtcaagcg
     6241 gttcgcccat gcccataaac acaatattgg taagcgagtt aaattcttcg atactacgca
     6301 tttggttcaa aatctgattg gctgttaggt tgcccgaaaa gccttgtttg ccagtagcac
     6361 aaaacaagca gttcatctta catccaattt gcgaagatat acagagggta ttacgttctt
     6421 tatcgggtat aaatacactt tcgataaaat gcttattacc aacctgaaac acatattttt
     6481 ttgtgccatc actagaaaca gaaacttgta caggtttttc gatacccacg caataattgt
     6541 ctgccaactt tttgcggttt gctgccgaaa tgttcgacat ttcttcaatg tctgagattt
     6601 cttttttata gagccaatct gttatttgtt tagctgcaaa agcaggcaac ccattggcgg
     6661 atacaatttc tgttatctcg cttagattct taccgaataa tgctttttta atcacgggtt
     6721 taattttttt ttaatgacac aaagatataa aaattacatc aatttgggaa atatttatca
     6781 ggatatcatt ttcagctttc atatacgtgt tgttacaaca aaaaacagga acactatttg
     6841 cattcctgtt tgatatgagc aatatctgat atggacttat tttgttccta tttgtactat
     6901 ttcttttttg ttcgatgttt gcactatgac attggcaaat tgctgaatag catgctcgtc
     6961 aattttttca agaatcgtta ggtaatctgt atgaatatcg agattggtaa taattttctc
     7021 aatttcaaca ttttgccaat accagttttc aattaaattt tcttggtatt gttttgttaa
     7081 aaattctttt actttttgca aatcttgtgt tcgtggacct tgacttgcaa tggcaaggaa
     7141 ttcttggttg ataatttcaa ctaactttga tcgttttgat tcgtcggtat caaaataggt
     7201 ttcaagtgta aaacgattat acggaatagc atctaaattg ccatcaacag atacgccata
     7261 agttcctcct tcatcttcgc gtactttttc tgtatataca atgtctaaga tttgacttaa
     7321 aatggtaagt tttattctgt tttctaacgt ataaggtatt tttcctgttg tgtattgtat
     7381 cgtgcttgac tttggaactt ccattatttt attgtaatgt agtgtgttgt ttccttcttt
     7441 tatggttact cctgcatctt tccaagtttc ttttttacct tttgcgggta gggtagcaat
     7501 gtatttggta agtaacggac gtaaggtttc gctatctatg tttccaacaa ttacaaacga
     7561 aaaatcggca gcattggcat aacgttctga taaaattttc attccttttt gatagtcgat
     7621 agttgacaaa tcggtcgatt ttaatcgtgt catcaaagga tggctatcgt atagtacatg
     7681 atttatggtg tctataaatg tcatgtacgg actattttct ctgtttttta atacagccaa
     7741 ttggcggttg atatatgcct gaaatgcgtc ttcgtcggtt cgtatgcctg taaaatgtaa
     7801 gtaagctaat tgaaacaaca tttctacatc ttttttaccc gaataaccgc ttaatgattc
     7861 tgaatacgta tttagttgtt tgcttaggcg aagatttttt cctgacaatc ttttttgtaa
     7921 atcaatggca ctaaaattgc ctaatccact aagagtagaa agttcgggca gtacctttaa
     7981 ggtggttgca tactgtttat cgaccaacga gtatccaccg tggctatagg cttgaaacaa
     8041 tatttcgtcg tctttgtggt tggtaggaac taatcgtacc gtaacattat tggataatat
     8101 aaagtcagtt gtaccgttgc tgtgtgtaat ctccttttta attttgcctg gtttgggtaa
     8161 tttgcttatt aatggctcgt tcgacacgtt ttcggtatat ggttcaatcg ttgcacgttt
     8221 tacttcttca attcctgata agatttcttc ggctgtaggg aaactaattt tttcatcttc
     8281 tggaccttgt accacaatta ccatattgcc cgtagtagaa gcctgcttta caatgttatt
     8341 tatcaattca agaggaatca tttctgctaa ttgtttatac gtttcaaagg tatattcaat
     8401 gcttggtatg ggcgtgtttt ctaaaaaatg attgatatat ttaggaacgt attcataatt
     8461 ttgttgtttc tctctttcgt tatattgatt ttctatgttt ttaagaatgt tcgctttctc
     8521 acgttcgtat tcggcaatgg taaatccaaa ttgtctcatc cgttcatttt cggtaatcaa
     8581 cgatttaatg gcagttgtag caccttgttc agaagcaatg gcatacaatg ttcttgcgtc
     8641 tttggtattc gacacccaaa agttatctgt ataggcataa gcttgtgtaa aaggcggatt
     8701 tggtttttgt atcatttcat taaaacgcga ccccatcatt ccgcttacgg cagcatgtac
     8761 atattcttgc atcatatacg caatcgttcc tttcatttcg gtaggaaaca catcttgttt
     8821 aatggacact tctaccgata tggcagttgc ttctttatca cgtgcaaaag cgataatagg
     8881 ctcttggttg tcggttacag catatacagg acgaggaata ggattagaag gcatggctat
     8941 tgtacctaga gttgctttta gtttagcctc cacttgttga ggatcgatat ctccaacaat
     9001 aattatagct tgtaaatcag gtctatacca ttttttgtaa aaggctctaa gttcatcagg
     9061 tttaaaattg cggataacat caatatgtcc aattggcaaa caatcggcat acttatcgtt
     9121 gccataaatg gtaggcaaaa tggtttctgc catacgcaac caagcactat ttctacttcg
     9181 ccattcttct tcaattacac ctctttcttt ttcaatttcg gaatcagata aggtaatgcc
     9241 atttgcccaa tcgcggagta tcagtaggca cgaatctatt accgtttccc ttaccacagg
     9301 aacattggta accatataca ctgtttgctc aacgctggta taagcgttta cgttttcgcc
     9361 aaatttaaca ccaatgcgtt gtaaataatt taataaatcc ttgttgggga aatgggttgt
     9421 accattaaac gccatgtgtt ctaaaaaatg agccaatcca cgttcgttgg gttcttctat
     9481 aacagaacca actttttgag caatataaaa gtcggctctt tctttaggca aactgttttt
     9541 gcgaatataa tacgtcatcc cattgggtaa ttgcccaata agcacttgtg ggtcggtagg
     9601 aatgatgggt aattgctgct gttgtgccaa acagataaca gcaaaaagta atagaaaaaa
     9661 tgatgcaatt agttttttca tttaggtgta gattaaatta ttgaagttgt ttattcgtat
     9721 gttttgtgta gagaatagat gttattttta gcaaactgat gatgcatata gcaaaagagc
     9781 accatagcat aaaagatgct ggaattaaaa agacttcata ttgatattcg ggcaataaat
     9841 agatatactc attcaagata aaaacatata tccacggtat aattcccact aattgcaaga
     9901 gccaaccaat ccataattta ttttcggcaa taataaatat gctagcagcc aatagtacaa
     9961 tcataacaac gaaatatgaa aacgaaggcg cccaagagcc taaatagacc tgaaaagtgt
    10021 gaacgtaatc gatgtcgtca aggatgtatc tcattattga cgctatcgtt aatagtatta
    10081 gaaataatac attaaatatc agtggtataa ccgttaaaat ggtgtttact attttattat
    10141 tcatataaaa agtggcttaa aaaatcattg ttgtatgtta ctatttcagt ttctttatcg
    10201 ttggcatata cagttacaaa tccttttgtc gtattttcga tacttacaat ttctttattc
    10261 tttattgttg gaaaacgaat gctacctaga tacgctttag ttgtaaggga gtaaatatca
    10321 agcattctat ctttttttgc tttattccat tgtttgcata cataagttgg cgttaacaag
    10381 tagataaatt tgtcatctat acaggctttg tcaataccgc aattgagcgg attgagcagt
    10441 acattgtgcc ctttatatga aggttctcct attggaaact tcctgaaatc ttgatgtgcc
    10501 gtatattcta agtttccatc cattaacaga aaataactat tgtagcggta gtaggcaagt
    10561 acttgtcctt gcttattggt agcaaatttg ttggtaaaag ttattgtacc ttttgcattc
    10621 gtaattgaat ctttcagatt cgttatttct ccaatattga taaagtttat tactgtatca
    10681 ttataagcat cgtataattc cattctatac gtattactgt ttttgttata tccaggtatt
    10741 ataaatttat tatttccaac atatagaata tcgcttaact catctatttt tctatcgagt
    10801 aaaagttcgt tgttattaga aaatttttgc agcaaatgct gtctcccatt aaagatgtct
    10861 acccagaatg tgctgtcttc ttgaataaat tgatataaaa cgccattgta tgtgagtgtt
    10921 gtatcttgca ataatgtacc ttcttgatta tacgttgcta atttgttgtt atagctcaaa
    10981 g
//
LOCUS       DAOSRP010000059        10862 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5932_length_10862_cov_4.888868, whole genome shotgun sequence.
ACCESSION   DAOSRP010000059 DAOSRP010000000
VERSION     DAOSRP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_10862_cov_4.888868"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..792)
                     /locus_tag="PLJ82_04940"
     CDS             complement(34..792)
                     /locus_tag="PLJ82_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOR40885.1"
                     /translation="MKVVKFAILVPIVFVLACSSKEPKLADVVDTGVVGKMIKVEGGK
                     FMMGCSDCAVDEAPVREVTLSTFYIGEFEVLQEEWIAIMEEENPSYHQGDSLPVEQVS
                     WNQVSTFITKLNEKTGKTFRLPTEAEWEFAARGGIKTMGYTYSGSNDINEVAWYLDNN
                     EEQETQKVGQKLPNELGLFDMSGNVAEWVQDRYSVYSEAAETNPAGPSSGTTRVCRGG
                     RCDHEASDCTVTARRSDRPANKYYNLGFRLVMVP"
     gene            complement(811..3543)
                     /locus_tag="PLJ82_04945"
     CDS             complement(811..3543)
                     /locus_tag="PLJ82_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR40886.1"
                     /translation="MKKSFFLFFLFLFSLSFFAQQLVTTTPAFIAQNYAGEIKVVFDA
                     TQGNAAMLGSATCYAHAGVILTSGPGWKKAPVWLDNASKYQLVPIGTDLWELTIPNLS
                     SYYNLAEGEVIAQLAFVFRNADGTKQGKTAAGGDIFIDLYDPSVLSMSVTNPSTDAVI
                     NKDASLVVQANVSMSSKIELFINSQLVKTEETATSISYSFDTSVAGDYTVKIKATNGE
                     NSVEISREICVKSPVQYAPVPTGMLEGINYDAQNNSVTLVLRAPLKENVFVIGDFNEW
                     KIKNQYQMFRNEENGILWWLTIDNLNPDTEYAFQYLIDGNLKVSDAYTEKVIDPWNDK
                     WIPTSVHPRLTYPEKADGLVSVFKINRPQYQWEVPDFQVPAKEDLIIYELLIRDFTTE
                     GTIKAAMGKLDYLQNLGINAIELMPIQEFDGNDSWGYNPNHYFAYDKAYGTQEDYKRF
                     IDECHKRGIAVFIDKVFNHATGVNPFAKMYWDGNATAANNPWFNVVAKHPFNVFQDFN
                     HEYEGTRQFFKRVLAYWIKEYNVDGYRMDLSKGFTQTNSGSDAGKWGKKDISRINIIK
                     DYHQAVKEANPNAVFILEHFADYDEEVILSTEGMLPWRNMTSNYGQATMGYSSNSNFV
                     DTNGKGGMFTNGWVGFAESHDEERLGYKMIAWGAGDLKTNESARLKRVPLVMAFVSFI
                     PGPKMLWQFQEMGYDYSIEYGGRTSRKPIPWTAFNSNQAKQEAYEASAKIISLRTNYP
                     QVFKEENVTVQATASYWSNGRRIVAQHPDMNVVAIGNFDATATITATPGFTKTGTWYE
                     LLSGTVLNVKNVNRTISLAPGELHVYTDRIPVSVPETLQDETMTYVYPTIAKDYIYVS
                     SLQDILQVNIYSLQGVLVKSEANSEIAISDLYSGYYIVQVVQADGVSIHKINKQ"
     gene            complement(3791..6826)
                     /gene="secDF"
                     /locus_tag="PLJ82_04950"
     CDS             complement(3791..6826)
                     /gene="secDF"
                     /locus_tag="PLJ82_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOR40887.1"
                     /translation="MQNKGLVLSFTIALVLVCLFYLSFNVVTSHYDKQAKEFAQGNVM
                     EEYRYLDSLSTEKVWLNYTLKECREKELNLGLDLKGGMNVIIEVSVPDILKTLAGNST
                     DETFTKAIQLAHQRQATSQKGFIDLFQEAFQEIDKDARLAAIFSTYELKDKVNLKSTN
                     AEVIAVIKKEVQATVDNSFNVLRTRIDRFGVVQPNIQRLETNGRILIELPGVKEPERV
                     RKLLQGSANLEFWETYTLPEVYESLVTANNVIKESQKDKIPVVETPTSVADSTSEEKV
                     EEQKDNVNSLLSQLEQPKDTLSEDDFSKENPLFSVLQLNVQNGQLVPTPAVGVAHQKD
                     TALVKSYLNLRQVKEVLPRNVEFKWSVKSIDTKGNFFELIAIKVTSRDGKAPLEGSAI
                     SDARSDFGQFSSNSVVSMTMNPEGARTWARLTKENIGRSIAIVLDDMVYSYPNVNTEI
                     TGGRSEISGNFTVEEAKDLANVLKSGRMPAPSRIVQEDVVGPSLGQEAIQKGLLSFAI
                     AFIGVMLYMILMYGVVPGLVVDGALLFNVFFLVGILASFKAVLTLPGIAGIVLTLGMA
                     VDANVLIYERIKEELAAGKTHKSAIADGYKNALSAILDSNITTIITAVILFYFGTGPI
                     KGFATTLIIGIVTSFFTAVFVTRWAYETLLDKNLTEKLSFTTKVSANFLSNVNLDWVK
                     LRKVGYILSITFMVVAIFSLAFHGLNQGIDFTGGRNYVIKFEESVSTQDLQRKLAPGF
                     EDASVSVITIGAVSENQVRISTNYKIQDNSDTVDDEIENVLYEQLKPFIKNQTVTRDM
                     FVKGYVINEDGDAQLSSAGSVRPLGVQSSQKVGPAIADDIKNAAYLAIFFSLIGIGLY
                     ILLRFKDISYSASIVVSLIHTSVFVLGVYSLFYSVMPFSMEIGQSFIAAILTVIGYSV
                     NDTVVVFDRLRETIGLYPKRDRKEMFNYSVNATLSRTFNTTFSTLLVLIIIFLLGGDT
                     IRGFVFALLLGITIGTYSTLFVSVPLAYDIQMKKLNKKK"
     gene            complement(6960..8009)
                     /gene="queA"
                     /locus_tag="PLJ82_04955"
     CDS             complement(6960..8009)
                     /gene="queA"
                     /locus_tag="PLJ82_04955"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOR40888.1"
                     /translation="MKLSQFKFKLPEELIATHPTKFRDESRLLVLNKKTGEIEHKVFK
                     NVYEYFSENDVFVFNDTKVFPARMFGNKEKTGARIEVFLLRELNRELKLWDVLVDPAR
                     KIRIGNKLYFGDNDELVAEVIDNTTSRGRTLRFLYDGNYETFKQLLYSLGETPLPRSL
                     NREAEPEDAERYQTIYAKNEGAVVAPTAGLHFSRELLKRMEIKGIHFANVTLHAGLGS
                     FRDIDVEDLTKYKMDSEQMMVTNEACQIVNAAKENRQQVCAVGTTVLRSLETAVSTNG
                     MIKPFDGWTNKFIFPPYDFTVATSMISNFHMPYSMLLMLVAAFGDYDYVMHAYSEAVK
                     EKYRFGTYGDALLII"
     gene            complement(8073..8756)
                     /gene="truB"
                     /locus_tag="PLJ82_04960"
     CDS             complement(8073..8756)
                     /gene="truB"
                     /locus_tag="PLJ82_04960"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR40889.1"
                     /translation="MNFIEGEILVFNKPLTWTSFALVHRVRAMICRKLKVKKLKVGHA
                     GTLDPLATGVMIICTGKATKRIEEFQYQTKEYIATLLVGATTPSFDLETKIDNVYPTE
                     HIKKETVQQVLSQFVGSIRQVPPVFSAVKVDGKRAYEYARNGEEVEIKAKELVIDEIE
                     LIDYSLPKITIRVVCSKGTYIRALARDIGVALQSGAHLVALERTCIGEYRIENCMTLE
                     NFQQIVDSE"
     gene            complement(8756..9613)
                     /locus_tag="PLJ82_04965"
     CDS             complement(8756..9613)
                     /locus_tag="PLJ82_04965"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOR40890.1"
                     /translation="MSIVETVIIAIVEGLTEFLPVSSTGHMIITQALLGVESTEFVKA
                     FTVNIQFGAILAVVVLYWQRFFKLNDTSSNYSIANQSKWKQLLYKFDFYVKLFVAFLP
                     AACIGFFAGEYIDMMLESVEVVAVMLLVGGIFMLFIDKLFNKKSDNQAITYKRAWIIG
                     FAQCIAMIPGVSRSMASIVGGLATKLDRKNAAEFSFFLAVPTMAAAAGYTLMKLVMDE
                     RTSAMLVDNLVVLLIGNIVAFVVAIIAIKFFIGFITKYGFKAFGWYRIIVATVLLILL
                     VANVDLAIA"
     gene            complement(9610..9852)
                     /locus_tag="PLJ82_04970"
     CDS             complement(9610..9852)
                     /locus_tag="PLJ82_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOR40891.1"
                     /translation="MKKEGDLSKRLGRINLKLIFIGVVVIIIGLVLMSVGGISTEEVF
                     AEDIFSVRRIVVAPMMVFFGFLFLVFAILYTPKTKA"
     gene            complement(9876..10760)
                     /locus_tag="PLJ82_04975"
     CDS             complement(9876..10760)
                     /locus_tag="PLJ82_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR40892.1"
                     /translation="MRKNLSSKPALRHLVNARVTTVISISLVLFLIGITSLLVLLANQ
                     LSIHVRENLAISIVMKDNVNEVEIQKLQKMLDATQYVKSTEYIDKETAAKELAAELGE
                     DPIAFLGYNPLLASLEVKLHSEYTHNDSIAKIETELKNFPEIKEVIYQKNLMQAVNEN
                     VSRITVFLLGISILLLFISFALINNTVRLSIYSKRFLIHTMKLVGATGSFIRQPFMIQ
                     GIFTGIVSACIAMGLLSLLVVSFQTELGGIITLESIDFMLVSFVVMIAVGIIITFVST
                     YFAVNRYLRIKLNDMYYM"
BASE COUNT         3501 a         2228 c         1621 g         3512 t
ORIGIN      
        1 atcggctttt gtttattgaa attagaatat ctgctatggc accattacca gcctgaatcc
       61 taaattgtaa tatttatttg caggtctatc actgcgtctg gcagttaccg tacagtcgct
      121 tgcctcgtga tcacatcttc ctcctctaca cacacgagtt gttcctgagc ttggtccagc
      181 agggttcgtt tctgcagctt cgctatacac cgaataccta tcttgtaccc attcggctac
      241 gtttccactc atatcaaata atccaagctc gttgggaagt ttttgaccaa ctttttgggt
      301 ttcttgttct tcgttattat ccaaatacca agccacttcg tttatatcat tgcttccgct
      361 ataggtatat cccatcgttt taattcctcc acgtgcagca aactcccatt cagcctcggt
      421 tggcaaacga aatgttttac ctgttttttc gtttaacttt gtaataaatg tggatacttg
      481 attccacgat acttgttcca caggcaggct atctccttga tgatacgatg gattctcttc
      541 ttccattata gctatccatt cttcttgtaa cacctcaaac tctccaatat aaaaggttga
      601 aagggtaact tcacgtacag gagcttcatc aactgcacaa tcactacaac ccatcataaa
      661 ttttccgcct tcaactttaa tcatttttcc gaccacacct gtatctacta catctgccaa
      721 ttttggctct ttggacgaac acgccagaac aaatacaata ggaacaagaa tagcaaattt
      781 aactactttc atacgatttt tatttgagta ttattgttta ttaattttat gaatagaaac
      841 gccatctgct tgcactactt gaacaatgta atatccacta tataaatcac ttattgcaat
      901 ttctgagttt gcttctgatt taactaaaac accttggagc gaataaatat ttacctgcaa
      961 aatatcttgt aacgaagaca catatatata atccttggct atagttggat acacataagt
     1021 catcgtttcg tcttgtaacg tttcaggcac acttacaggt attctatctg tatacacatg
     1081 taattctcca ggagctaacg atattgttct attcacattc ttaacattca aaaccgttcc
     1141 tgataataat tcataccatg ttccagtttt agtaaaacct ggcgttgctg ttatagttgc
     1201 agtagcatca aagtttccta tagctactac attcatatct gggtgttgtg ctactattct
     1261 tcttccattt gaccaatagg aagcagtagc ttgtacagta acattctcct ctttaaaaac
     1321 ttgagggtaa ttggttcgta aacttatgat tttagctgat gcctcataag cctcttgttt
     1381 ggcttgatta gagttaaacg ctgtccaagg tattggttta cgtgatgttc ttccgccgta
     1441 ttctatcgaa taatcatatc ccatttcttg aaattgccat aacatttttg gaccaggaat
     1501 gaatgacaca aatgccatta ccaatggaac tctttttaat ctggctgact catttgtttt
     1561 taaatcacca gcaccccacg ctatcatctt atagcccaga cgttcttcat catggctttc
     1621 tgcaaaacca acccatccgt tggtaaacat tcctccttta ccattcgtat caacaaaatt
     1681 actgttactt gaatagccca tggtagcttg accataatta ctagtcatat tcctccaagg
     1741 caacatgcct tctgtagaaa gtataacttc ttcatcataa tcagcaaaat gttcaagtat
     1801 aaaaacagcg tttggatttg cctcttttac ggcttgatgg taatctttaa taatattgat
     1861 acgcgatata tctttctttc cccatttacc tgcatcacta cctgaatttg tttgagtaaa
     1921 tcctttcgac aaatccattc tatatccatc cacattgtac tcttttatcc aatatgcaag
     1981 tacccgttta aaaaactgtc tagttccctc gtactcgtgg ttaaaatctt ggaaaacatt
     2041 aaatggatgt ttcgcaacca cattaaacca agggttgttt gcagcagtag catttccatc
     2101 ccaatacatt tttgcaaaag gattaacccc tgtagcatga ttgaacactt tatcaataaa
     2161 tacagctata ccacgtttgt gacactcatc aataaaccgt ttataatctt cttgtgtgcc
     2221 ataggcttta tcataagcaa aataatgatt tggattgtag ccccaactat cattgccatc
     2281 aaactcttga attggcatca attcaatcgc atttatacct aaattttgca aataatctaa
     2341 cttgcccatt gccgctttaa tggttccttc tgttgtaaaa tcgcgaataa gcaattcata
     2401 aataatcaaa tcttctttag ctggaacttg aaaatctgga acttcccatt ggtattgtgg
     2461 tctatttatt ttaaagacag aaaccaaccc atcagccttc tctgggtacg ttaatcgtgg
     2521 atgaactgat gttggtatcc acttatcatt ccatgggtca attacttttt cagtatacgc
     2581 atcactcact tttagattac catctattaa atactgaaac gcatattcgg tatctggatt
     2641 aaggttatca atggttaacc accacaaaat gccattttct tcattgcgaa acatttggta
     2701 ttggttcttt attttccatt cgttaaaatc acctataaca aacacatttt cttttaatgg
     2761 ggctctaagt actaaagtaa cactattatt ttgagcatca taattaatcc cttctagcat
     2821 acctgttggt acgggtgcat attgtacagg agatttcaca caaatctctc tacttatttc
     2881 aacactgttc tctccgtttg ttgctttaat tttcacagta taatcaccag caacacttgt
     2941 atcaaagcta taagaaatag aagtagcggt ttcctctgtt tttactaact gactgtttat
     3001 aaatagctct attttagaag acattgatac atttgcttgc acaaccaaag acgcatcctt
     3061 atttattaca gcatctgtcg aagggttcgt aacgctcata ctcaacacag aaggatcgta
     3121 taagtcaata aagatatcgc caccagcagc tgtcttgcct tgcttggttc catcggcatt
     3181 tctaaataca aaagccaatt gtgcaattac ttcgccttca gccaagttgt aataagatga
     3241 caaatttgga attgttaact cccacaaatc tgtcccaatg ggaacaagtt gatatttaga
     3301 cgcattgtca agccaaactg gagctttttt ccatcctgga cctgaggtta gtataacccc
     3361 tgcatgcgca taacatgtag ctgacccaag catagcagca ttaccttgag tagcatcaaa
     3421 aacaactttt atttcgccag cataattttg agcaataaat gccggagtag tcgtaactag
     3481 ttgctgagca aaaaaagaaa gactaaaaag aaacagaaaa aatagaaaaa aacttttttt
     3541 catacgatga tgttttcaag tataaagaca cacacctcac tccacaaact atgtatcatt
     3601 tataaaaatg tatacaaaaa caagctgaat gccgtctact tattgtgaac aaatataatt
     3661 aatatacaat aataattgca aaaatctttc gcaagatacg aaatattttt ggataaagca
     3721 attgattaga tgcattttaa caaaaaagga ctcacttata tgaatccttt tttgttaaaa
     3781 tactataccc ttacttcttc ttatttaact tcttcatctg tatatcgtac gctaatggta
     3841 cagaaacaaa caatgtagaa tacgttccaa tcgttattcc taataacaaa gcaaacacaa
     3901 atcccctaat tgtatctcca ccaagtaaga aaataattat caaaaccaat aaggtagaaa
     3961 aagtggtatt aaatgttctg gataaggttg catttaccga atagttaaac atttcttttc
     4021 tatcccgttt cggatacaac ccaatcgttt ctctcaatcg gtcgaatact actaccgtat
     4081 catttaccga atacccaatt acggtaagta tagcagcaat aaatgattgt ccaatttcca
     4141 tagaaaaagg catcacacta tagaacaatg agtacactcc caatacaaat acagaggtat
     4201 gaattaaaga aaccacaata cttgctgaat aagatatatc tttaaatcgc aaaagaatat
     4261 acaatccaat accaatcaat gaaaagaaga tagccaagta ggctgcattt ttgatgtcgt
     4321 cagcaatagc tggaccaact ttttgcgaac tttgcacgcc taatggacgc actgaaccag
     4381 cactagacaa ttgggcatcg ccatcttcat ttataacata ccccttcaca aacatatcac
     4441 gagtaactgt ttgattcttg ataaatggct ttaattgttc atacaagaca ttttcaattt
     4501 catcgtccac agtatcactg ttatcttgaa ttttatagtt tgtagatatc cgtacttgat
     4561 tttcagaaac agcaccaata gtaattacag atacagaagc atcctcaaaa ccaggagcca
     4621 gcttacgttg taggtcttgt gtacttacgc tttcttcaaa ttttatcacg taattacgac
     4681 caccagtaaa atcaattcct tgattcaaac catggaaagc caacgagaat atagccacta
     4741 ccataaaggt aatagaaaga atatagccta ctttacgtaa tttcacccaa tcaagattga
     4801 cgttacttaa aaaattggct gaaaccttgg ttgtaaaaga taatttttct gtaagatttt
     4861 tatccaataa cgtttcgtaa gcccaacgag taacaaatac ggctgtaaaa aatgatgtaa
     4921 cgatacctat aatcagtgta gtagcaaatc cttttattgg accagtacca aaataaaata
     4981 atattacggc agtaataatg gttgtaatat ttgaatccaa gatagcagat aaagcatttt
     5041 tgtaaccatc ggcaattgct gatttatgtg tttttccagc tgcaagttct tctttaatac
     5101 gctcataaat caacacattg gcatctaccg ccatacccaa cgtaagcaca atacctgcta
     5161 taccgggcaa ggttaatacg gctttaaacg aggctaaaat accaacaagg aaaaatacat
     5221 taaataataa ggctccgtca actactaacc caggcacaac accatacata agtatcatat
     5281 aaagcattac gccaataaat gcaatagcaa aagaaagtaa gcccttttga atggcttctt
     5341 gaccaagaga tggtcctacc acgtcttctt gaacaatacg tgatggggca ggcatacgac
     5401 ccgacttcaa tacgttagct aagtcttttg cttcttctac cgtaaaatta ccactaattt
     5461 cagaacgtcc acccgtaatc tcagtattta cattaggata agaatagacc atatcatcta
     5521 atacaatagc aatacttcta ccaatatttt ctttggttaa tcgtgcccac gtacgtgccc
     5581 cttctgggtt cattgtcata ctcaccacgg aatttgacga aaactgtcca aaatcactac
     5641 gagcatcact aatagcactt ccttctaatg gagccttgcc atctctactt gtaactttaa
     5701 tagctatcaa ctcaaagaaa ttccctttgg tatcgattga ttttacagac catttgaact
     5761 ctacgttacg aggaagcact tcttttactt gacgtaaatt taaataactt tttacgagag
     5821 ctgtgtcttt ttgatgagcg acaccgacgg caggagttgg caccaactga ccattttgaa
     5881 catttaattg caagacactg aataagggat tttcttttga gaaatcatct tcagaaagag
     5941 tgtctttagg ttgttcaagt tgagataaca aagagtttac attatctttt tgttcttcta
     6001 ccttctcttc cgaagtagaa tcagcaacac tagtaggtgt ttcgacaacg ggtattttat
     6061 ctttttgaga ttcctttatt acattattgg cagttactaa agactcgtac acctctggta
     6121 aggtatatgt ttcccaaaat tctaggttag ccgaaccttg aagcagttta cgcacacgtt
     6181 ctggttcttt tacaccaggc aattctatta atatacgtcc attggtttca agacgttgaa
     6241 tatttggttg aactacccca aaacggtcaa tacgagttct caatacattg aatgaattat
     6301 caacagttgc ttgtacttct tttttgataa cggcaataac ttcggcatta gtactcttta
     6361 gattaacttt atcctttagt tcataggtac taaatatagc tgctaaacgg gcatctttat
     6421 ctatttcctg aaaagcttct tgaaacaaat caatgaaacc tttttggctt gttgcttgac
     6481 gttggtgtgc taactgtatg gctttagtaa atgtttcgtc agtactatta cctgcaaggg
     6541 tttttagaat gtcgggcact gacacttcaa tgataacatt cataccgcct tttaggtcta
     6601 agcctaagtt taattctttc tctctacact ctttcaaggt atagttaagc cacacttttt
     6661 cagttgaaag agaatctaaa tagcggtact cttccattac atttccttgt gcaaactctt
     6721 ttgcttgttt gtcgtaatgt gatgttacaa cattaaacga caagtaaaaa aggcacacta
     6781 agaccaaggc aatggtaaaa gatagtacaa gtcctttgtt ttgcatgttt gtattaaaat
     6841 ttaaaattaa tattcgtttg ttttatattt ttgtgaaata gggtgcaaat atagtttttt
     6901 ttttacagaa aagctatatt actgtaaaat aaaccgatat aatccataca atgagtgctt
     6961 taaataataa gcaacgcatc tccataagta ccaaaacggt atttttcttt tacagcttcg
     7021 ctatatgcat gcattacata gtcataatcg ccgaaagcgg ctacaagcat taataacata
     7081 gaatatggca tatggaaatt actaatcatg ctagtggcta ctgtaaaatc atacggaggg
     7141 aaaataaatt tatttgtcca accatcaaaa ggttttatca taccattggt actaacggct
     7201 gtttctaacg aacgtaaaac ggtggttccg acagcacata cctgttgacg attttctttt
     7261 gcagcattta caatttggca agcctcattc gttaccatca tttgctccga atccattttg
     7321 tacttagtca aatcttccac atcaatgtcg cgaaaacttc ccaatccagc gtgtagagtt
     7381 acgtttgcaa aatgtattcc cttaatttcc atacgtttaa gcaattcgcg actaaaatgc
     7441 aacccagcgg taggggcaac cacagctcct tcattttttg catatatagt ttgataacgt
     7501 tcggcatctt ctggttctgc ttctctattc aaagatcgtg gtagtggtgt ttctcccaaa
     7561 gaatatagca attgcttaaa tgtttcataa ttcccatcgt ataaaaaccg caaagtacgt
     7621 cctctagaag tagtattatc aattacttcg gcaactaatt catcattatc accaaaatac
     7681 aacttattgc caatacgaat tttgcgagcg gggtctacca atacgtccca aagtttaagt
     7741 tctcggttta attctcttag taagaacacc tcaatacgtg ctcctgtttt ttctttattt
     7801 ccaaacatac gtgctggaaa cactttggtg tcgttaaata caaatacatc gttttcggaa
     7861 aagtattcgt acacattttt aaatactttg tgctcaattt caccagtttt tttattcaac
     7921 accaataagc gcgattcgtc gcgaaatttt gtaggatgtg ttgcaattag ctcttcgggc
     7981 aacttaaatt taaattgtga aagtttcatt ttgattgttt ttgttattac tattctctat
     8041 taagatatgt acaaattatg aagccacata tcttattctg aatcaactat ttgctgaaaa
     8101 ttttctaaag tcatacaatt ttctattcta tactcaccaa tgcacgtacg ctctaaggca
     8161 acaagatgag caccactttg caaggctact ccaatatccc gagccaaagc ccttatatat
     8221 gtacctttgc tacacactac ccttatggta atttttggca acgaataatc gattaattca
     8281 atttcatcta ttaccaattc tttggctttt atctctactt cttctccatt acgggcatat
     8341 tcgtatgctc tttttccatc aacttttacc gccgaaaaaa caggtggtac ttgccttatt
     8401 gagcccacaa actgagaaag aacttgttgt accgtttctt tctttatatg ctcagtagga
     8461 tacacattat ctatttttgt ttctaaatca aacgacggag tagttgctcc aactagcaac
     8521 gtagcaatat attcttttgt ttgatactga aactcttcta tacgtttggt agctttgcct
     8581 gtacaaataa tcattacccc agtggctaag gggtcgagtg tacctgcatg accaaccttt
     8641 aatttcttta cctttagttt tctgcatatc atcgcacgca cacgatgtac tagagcaaaa
     8701 gatgtccacg ttagcggttt attaaaaacc aaaatctctc cttcaataaa attcattagg
     8761 ctatagcgag gtcaacgttt gcaactaaca agattagtaa aacagtagct actatgattc
     8821 tataccaacc aaatgcttta aagccatact ttgtaataaa cccaataaaa aatttgatgg
     8881 caataatagc cacaacaaaa gctacaatat tacctatcaa caaaacgacc aagttgtcaa
     8941 ccaacatagc cgaagtacgc tcatccatta ctaacttcat caaagtatac ccagctgcgg
     9001 cagccatagt tggtacagcc aaaaaaaacg aaaattcggc agcatttttt ctatccaatt
     9061 tagttgctaa accgcctaca atagatgcca tagaacgcga cactccaggt atcatggcga
     9121 tacactgagc aaaccctata atccaagctc ttttatatgt aatagcttgg ttatctgatt
     9181 ttttattaaa taatttatca ataaaaagca taaaaatacc gcctacaagt agcattactg
     9241 caactacttc tacactttct agcatcatgt ctatatactc gccagcaaaa aaaccgatac
     9301 acgctgctgg caaaaaagct acaaaaagtt ttacataaaa atcaaactta tacaataact
     9361 gtttccactt actttggtta gctatagaat aattagaaga agtatcattc agtttaaaga
     9421 aacgttgcca atataaaaca acaacagcta aaattgcacc aaattgaatg tttacggtaa
     9481 atgcttttac aaattctgta ctttcaacac ccaataaggc ttgtgtaata atcatgtgcc
     9541 ctgtagaaga tacgggcaaa aactctgtta gcccctcaac aatggctata ataacagttt
     9601 caactatact catgctttcg ttttaggtgt atataaaata gcaaaaacga gaaataaaaa
     9661 cccaaaaaat accatcattg gagcaactac aatacgtctt acactaaaaa tatcctcagc
     9721 aaaaacctct tctgtagata tccctcccac agacattaat accaatccaa taataataac
     9781 aacaacaccg ataaaaatta atttcaaatt gatacggcct aatcgtttac ttaaatcacc
     9841 ttcttttttc atgttaactg ttttttttgt aaaaattaca tgtaatacat atcattaagt
     9901 ttgatacgca aataacgatt gacagcaaaa taagtagaca caaaggtaat tataatacca
     9961 acagcaatca ttaccacaaa tgacaccagc ataaaatcaa tactttcgag ggtaataatt
    10021 ccacctaatt cagtttggaa agataccaca agtaacgaca acaatcccat agcaatacat
    10081 gcggatacaa tacccgtaaa aataccttgt atcataaatg gttgacgaat aaagctccct
    10141 gtagccccca ctaatttcat ggtatggatt aagaaacgtt ttgaataaat acttaaacgc
    10201 acggtattat tgataagggc aaacgatata aatagcaata aaatagaaat gcctaacaaa
    10261 aagaccgtta ttctactaac attttcgttt actgcttgca ttaagttttt ttgataaata
    10321 acttctttaa tttctgggaa gtttttcagt tcggtttcaa tttttgcaat actatcgttg
    10381 tgtgtatatt cggaatgaag tttgacttcc aaggatgcta aaagaggatt atatcccaaa
    10441 aaagcgatgg gatcttcgcc tagttctgct gctaattctt tagctgcagt ctctttatca
    10501 atgtattcgg tagattttac atattgagta gcatccaaca ttttttgtaa tttttgaatt
    10561 tctacctcat tcacattatc tttcattaca atagatattg ccaaattttc acgcacatgt
    10621 atggataact gattggcaag taaaactagc aatgaggtaa taccaattaa aaaaagcacc
    10681 aaagatatac taataactgt tgttactcgg gcattaacca aatgacggag ggcgggtttt
    10741 gaagaaagat tttttctcat aattgtatgg taaggtaaaa taagcgtatc taatttttta
    10801 tgcttgtcta ttcttttttg aacctgcaaa gataatacct aaaaaagcga aagtcaatag
    10861 ta
//
LOCUS       DAOSRP010000060        10783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_5982_length_10783_cov_5.794463, whole genome shotgun sequence.
ACCESSION   DAOSRP010000060 DAOSRP010000000
VERSION     DAOSRP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5982_length_10783_cov_5.794463"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLJ82_04980"
     CDS             complement(<1..448)
                     /locus_tag="PLJ82_04980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843366.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR40893.1"
                     /translation="MERQAFQKIYTHLTNITKATVTLEAGDVGYEEMAIVHGRLAQVV
                     KIMGKMITLQVFSGTEGIPTDAEILFLGRPPQLKVSDELSGRFFNAYGDPIDGGPEIN
                     GEIRDIGSPSVNPVRRKQPSQLIATGIAGIDLNNSLVSGQKIPFFAD"
     gene            complement(462..2234)
                     /locus_tag="PLJ82_04985"
     CDS             complement(462..2234)
                     /locus_tag="PLJ82_04985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR40894.1"
                     /translation="MERKNKTTGKVVGIVANLVIVETNGPVAQNEICFIHLGQENLMA
                     EVIKIVGIKAYVQVFESTRGLKVGTSVTFEGHLLEVTLGPGILSKNYDGLQNDLDKMK
                     GVFLTRGEYTDPLERDSLWNFSPIAKVGDTVYAGSWLGEVMENSIPHKIMVPFKLEKK
                     YTVRSIVQAGTYTVDTTIATIKDDEGNEIAISMVQKWPVKMPIKAYDNKPRPFKLLET
                     GVRCIDTLNPITEGGTGFIPGPFGTGKTVLQHAISKQAEADIVIIAACGERANEVVEV
                     FTEFPKLEDPRTGRKLIERTIIIANTSNMPVAAREASVYTAMTLAEYYRSMGLKILLM
                     ADSTSRWAQALREMSNRLEELPGPDAFPMDLSAVISNFYARAGFVYLPNGTTGSITFI
                     GTVSPAGGNLKEPVTESTRKAARCFYGLSQARADSKRYPAIDAIDSYSKYLEYPEVQT
                     YLEKNLGKEWLGQVLQAKDILLRGKEAMEQINILGDDSVPLVFHDKYWKSELIDFIIL
                     QQDAFDKIDASCPMNRQQYMLTSVLKVANTEFKFDGFEEIGGFFKSIINIYKQMNYSE
                     FQSEKFTQYESELNTLVSNKQVNE"
     gene            complement(2218..3081)
                     /locus_tag="PLJ82_04990"
     CDS             complement(2218..3081)
                     /locus_tag="PLJ82_04990"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOR40895.1"
                     /translation="MSKNYYALVAGLPDIMAQDKKLLYTSVQMRDIICEEVSEEDMSL
                     VQLLFLPFDHLNIVNALFKKQVSWDERGNLASEIVEQLKDKKNIESVDLENVPSYIRD
                     FVERYYVEEQEINFYQAEFLLTEAYFEYLTTHTNSFVAALGYYYTSLNNLKVAYNGRK
                     HDVSYDKQLIGENELTHALRKSRTRDFGASTLVDNLESLLQIFEIENTIERELKIDNN
                     TWNFVEENTFFNYFSIERVMAFIVKLLIVERWLLLDEEKGKQMFNQLVTELQDGFQFS
                     HEYTLAYGKKK"
     gene            complement(3084..3695)
                     /locus_tag="PLJ82_04995"
     CDS             complement(3084..3695)
                     /locus_tag="PLJ82_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40896.1"
                     /translation="MQGKLQELTEKIYQEGVQKAQKEVEILLQAATQKAQNIEQEAQK
                     KAKAIIAEAEKKAELLDKHVQSELKMSVTQSTSALKQDLTNLLTAKAIEAPVKELFEN
                     TAFLQTLVQTVVSSWIAKGTYDLQVILPEKDKKELDAAFKKQLAKELSKGVELIFSDT
                     VKNGFKVVQSKEGYQLSFTDQDFINYFKAYLRPKTTELLFGKG"
     gene            complement(3850..4395)
                     /locus_tag="PLJ82_05000"
     CDS             complement(3850..4395)
                     /locus_tag="PLJ82_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40897.1"
                     /translation="MLLLNSFNVGITDYFSIGGGIEFLSTFISLSQGQFNPIMYFTPK
                     IGVPVAEKMSIGGGVLLASIPQFSLSSNTGLGLLYGLGTYGNKNTNVTATVAWGSIDR
                     NAFNKPLITVSGMTRVAKKTALVTENWLIPTDSSYEPIFSYGVRFFGENIAVDLAFIN
                     NWDIAQSLLIGVPYVNFAVKF"
     gene            complement(4515..4739)
                     /locus_tag="PLJ82_05005"
     CDS             complement(4515..4739)
                     /locus_tag="PLJ82_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40898.1"
                     /translation="MKKLHLFFFVLSISFVSLYAQNNTEETAIYYVTLKDKTHYLGTI
                     ESESTNYIVLYNASTGTRTEIPINQIQEKK"
     gene            complement(4759..5136)
                     /locus_tag="PLJ82_05010"
     CDS             complement(4759..5136)
                     /locus_tag="PLJ82_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40899.1"
                     /translation="MNTIYQTLIEEYGIETQKGFGIYYDNPQEVQKENLRSDVGCILN
                     LTDSAKIVQLSSKFLIKEIPAKKYLTATFPYKGQLSIFIGICRVYPALHHYVIKNNIP
                     PIGAVMEVYDVPNKQIHYRIVEH"
     gene            complement(5451..6356)
                     /locus_tag="PLJ82_05015"
     CDS             complement(5451..6356)
                     /locus_tag="PLJ82_05015"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOR40900.1"
                     /translation="MNKIKVAIVGYGNIGRFSLEAVQAAPDMELMGVVRRASSVGNVP
                     NELNGIKIVSNIAELGKVDVAILCGPTRSIPETAVELVKKGIRTVDSFDIHGEELWKL
                     RQSLDVAGKKTNAASIISAGWDPGSDSVIRTLLLAMAPKGITYTNFGPGMSMGHSVVA
                     RSKEGVKNALSMTIPTGTGVHRRMVYVELENGAKLEKVTEAIKTDSYFSKDETHVMEV
                     ASVDNLQDMGHGVLIERKGVSGKTQNQLFQFSMQINNPALTAQILISCARAVTKQAPG
                     AYTMPEIAPLDYLYGDRETLIKTLV"
     gene            complement(6370..7830)
                     /gene="gltA"
                     /locus_tag="PLJ82_05020"
     CDS             complement(6370..7830)
                     /gene="gltA"
                     /locus_tag="PLJ82_05020"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOR40901.1"
                     /translation="MALVDRSEAWREELRASMKNKERTNIERVHMNELDATIRSRNSE
                     EVNLGLTVEQALLEAKRCLDCANPTCMNGCPVSINIPKFVKNIERGEFLEAAKTLKET
                     SALPAVCGRVCPQELQCESQCIHIKMGKPAVAIGYLERFAADFERESGQISVPEIPHK
                     NGIKVAAVGSGPAGLSFAGDMAQRGYDVTVFEALHQIGGVLKYGIPEFRLPNKIVDVE
                     IECLRQMGVKFLTNCIVGKTITFDELSEEGFKGMFVGSGAGLPNFMNIKGENLIGVMS
                     ANEYLTRVNLMDAANPESDTPVYKGKSVAIIGGGNTAMDAVRTARRLGADRAMIVYRR
                     SEEEMPARIEEIKHAKEEGIEFLTLHNPIEYHGKERGRVTHMLLRKMQLGEPDASGRR
                     SPIAIEGATELIEVDMVIVSVGVSPNPLVPQSIQGLEVSRKGTVIVNENSMQTNIPAI
                     FAGGDIVRGGATVILAMGDGRKAAAAMDEYLKESEN"
     gene            complement(7814..8653)
                     /locus_tag="PLJ82_05025"
     CDS             complement(7814..8653)
                     /locus_tag="PLJ82_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOR40902.1"
                     /translation="MNKIISKEHFSANVVKLEVEAPMIAKSRRAGHFVLVKVGEKGER
                     IPLTITSSDLEKGTITLVIQKMGVSSTKICNLNEGDYITDLVGPLGKATHIEKFGTVV
                     CAGGGVGLAPLLPIVEALKKAGNRVITVLAARTKDLIILEDQLRKHSDEIVIMTDDGS
                     YGNKGLVTNGVENIINREKVDLCVTIGPAVMMKFVSLLTKKYEVPTLCSLNTIMVDGT
                     GMCGACRVTVGGKTKFVCVDGPEFDAHQVDFDEMLMRLNAYKDEETESMTNLKNNQHG
                     ISR"
     gene            complement(8755..10089)
                     /locus_tag="PLJ82_05030"
     CDS             complement(8755..10089)
                     /locus_tag="PLJ82_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOR40903.1"
                     /translation="MNAKNVIENLKKRFPNEPEYLQAVEEVLESIEEVYNQHPEFEKA
                     NLIERLCIPDRIFTFRVSWVDDKGNVQVNMAYRVQHNMAIGPYKGGIRFHSSVNPSIL
                     KFLAFEQTFKNALTTLPMGGGKGGSDFHPRGKSSGEVMRFCQAYLLELHRHIGPHTDV
                     PAGDIGVGGREVAYMFGMYKKLTHEFTGTFTGKGIEFGGSLIRPEATGYGNIYFLLNM
                     LATRNIDIKGKKCLVSGSGNVAQYTCEKLIELGAIPVTLSDSNGYIYDPEGITREKLE
                     YVMDLKTVQRGRIKEYADKYNCKYVEGGRVWAEKGDIALPSATQNELNGDDAKTLMAN
                     GCIAVSEGANMPSTPEAIEIFLKNKILYGPGKAANAGGVATSGLEMSQNAIGLSWSRE
                     EVDARLKGIMNNIHENCVKYGTEKDGFVNYVKGANIAGFMKVAKAMMAQGIV"
     gene            10419..>10783
                     /locus_tag="PLJ82_05035"
     CDS             10419..>10783
                     /locus_tag="PLJ82_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR40904.1"
                     /translation="MELATKYNPTDIESKWYQYWIDHALFASKPNDKEPYTIVIPPPN
                     VTGVLHMGHILNNSIQDILIRRARMQGKNALWVPGTDHASIATEAKVVNKLAEEGIKK
                     TDLTRDEFLKHAWDWTEKHG"
BASE COUNT         3177 a         2121 c         1761 g         3724 t
ORIGIN      
        1 ggtcagcaaa aaagggtatt ttttgtcccg ataccaagct gttatttagg tcgatacctg
       61 caattcctgt tgctattaat tgcgatggtt gctttctgcg aacggggttt actgatgggc
      121 taccaatatc gcgtatttcg ccattaattt ctggaccacc atcaataggg tcgccataag
      181 cattaaaaaa acgcccactt agttcatcgc ttacttttag ttgtggtgga cgacctaaaa
      241 aaagtatttc agcatccgta ggtatccctt ctgtaccaga aaatacttgc aaggtaatca
      301 tcttgcccat aatttttact acttgagcca atctaccatg tacgatagcc atttcttcgt
      361 aacctacatc gccagcttct aatgttactg tggctttggt tatattggtt aagtgggtat
      421 atattttttg aaaagcttga cgttccatat tttatctttt cttattcgtt aacttgtttg
      481 ttgcttacaa gcgtatttag ttccgattcg tattgagtaa atttttctga ctgaaattcg
      541 ctataattca tttgtttata tatattgatg attgacttaa agaaacctcc aatttcttca
      601 aaaccatcaa atttaaattc agtatttgca actttcaata cgctagtaag catatattgt
      661 tgtctgttca ttgggcatga ggcatcaatt ttatcaaaag catcttgttg caaaatgata
      721 aagtcaatta gctctgattt ccaatattta tcatggaata ccaacggcac actatcatcg
      781 cctaagatgt tgatttgttc catagcttct ttgccacgaa gcaaaatatc cttggcttgt
      841 aatacttgtc ctaaccactc tttccctaag tttttttcta gatatgtttg aacttcaggg
      901 tattcgaggt atttggagta actatcaata gcatcaatag ccgggtagcg tttactatca
      961 gcacgagctt gcgataagcc atagaaacaa cgagcagcct tacgtgtgct ttctgtaacg
     1021 ggttctttta agtttcctcc tgcgggtgat acagtgccaa taaatgtaat agacccagtg
     1081 gttccgttag gtaaatacac aaaccccgca cgtgcataga aattggatat tactgctgat
     1141 aaatccatcg gaaaagcatc aggtccagga agttcttcca atcggtttga catttcgcgt
     1201 agtgcctgag cccaacgaga ggtggagtct gccattaata atatttttaa acccatcgag
     1261 cggtaatatt ctgccagtgt cattgcggta tatacagacg cttcgcgtgc tgcaacaggc
     1321 atattagagg tattagcaat aataattgta cgttcaatca gttttctgcc agtacgtgga
     1381 tcttccaatt taggaaattc tgtaaatact tctactactt cgttcgcacg ttcgccacaa
     1441 gcggcaataa taacgatatc tgcttctgct tgtttagaaa tggcgtgttg caatactgtt
     1501 tttccagttc caaaaggacc aggaataaaa cctgtgccac cttcagtgat tggattgagg
     1561 gtatcgatac aacgaacacc tgtttcgagt agtttaaagg gtcttggctt gttatcgtat
     1621 gctttgatag gcatttttac gggccatttt tgcaccatgc tgatggctat ttcgttgcct
     1681 tcatcatctt taatagttgc aatggtggta tcaacggtat aagtacctgc ttgtacgata
     1741 gaacgaacgg tatatttttt ttctaattta aatggtacca taattttgtg aggaatacta
     1801 ttttccatca cttcgcccaa ccatgatcca gcatatacgg tatcgcccac ttttgcaata
     1861 ggagagaagt tccacaaaga atcgcgttct aatgggtcag tatattcgcc tctagttaag
     1921 aatacaccct tcattttgtc taaatcattt tgcaaaccat cgtaattttt tgacaaaata
     1981 ccaggaccaa gcgttacttc taataggtgt ccttcgaatg ttacactcgt accgactttt
     2041 agtccacgtg tgctttcaaa aacttgaacg tatgctttga ttccaacaat tttaattacc
     2101 tcagccatta aattttcctg accaagatga ataaagcata tttcattttg agcaacgggt
     2161 ccattggttt ctacaatcac caaattggct actataccaa caactttgcc agtagtttta
     2221 ttttttcttt ccatatgcta gtgtatattc atgcgagaac tgaaatccat cttgcagttc
     2281 tgttactaat tgattaaaca tttgttttcc tttttcctcg tctaagagca gccatctttc
     2341 tacaatgagc agtttaacaa taaaagccat aacacgctca atggagaagt aattgaagaa
     2401 agtattttct tcaacaaaat tccacgtatt gttgtcaatc tttagctctc tttcaattgt
     2461 attttctatc tcaaaaattt gcaacaggct ttccaagtta tccaccaaag ttgatgctcc
     2521 aaaatcgcgt gtgcgacttt ttctcagggc atgtgttagc tcattttctc cgattaattg
     2581 cttgtcatac gatacatcat gttttcgccc attataagct actttaaggt tatttagtga
     2641 tgtgtagtaa tagcctaatg cagcaacaaa ggaatttgta tgggttgtta aatactcaaa
     2701 ataagcctca gtaagtaaaa attcagcctg ataaaaattg atttcttgct cttctacata
     2761 atatctttca acaaaatctc ggatgtagct tggtacattt tctaaatcaa cgctttcaat
     2821 gtttttttta tcttttaatt gttcaacgat ttcagaggca agatttcctc tttcgtccca
     2881 agaaacttgt tttttgaata atgcatttac tatattaaga tgatcgaatg gcagaaacaa
     2941 taattgcacc aacgacatat cttcttcgct cacttcttcg cagatgatat ctcgcatctg
     3001 tacagaagtg taaagtaatt ttttgtcctg agccatgata tcgggtaatc cggctactaa
     3061 tgcatagtaa ttttttgaca ttcttatcct tttccgaaga gtaattcagt agttttaggg
     3121 cgtagataag ctttgaaata gtttataaaa tcttggtcgg taaaacttaa ttgataacct
     3181 tccttcgatt gaaccacctt gaatccattt tttacagtat cagaaaaaat caattctact
     3241 cccttgctca attcttttgc tagctgtttt ttgaaagcag catccaattc ttttttatct
     3301 ttttctggaa gaataacttg caaatcgtat gttccttttg ctatccaact acttacaaca
     3361 gtttgaacaa gagtttgtaa aaaggctgtg ttttcaaata attcctttac aggggcttca
     3421 atagctttcg ctgtaagcaa attagttaaa tcttgtttta gtgctgaagt ggattgagta
     3481 accgacattt ttaattcgga ctgtacgtgt ttatctagca gttcggcttt tttttcagct
     3541 tcggcaatga tagctttggc ttttttctga gcttcttgtt ctatattctg agctttttgg
     3601 gtggctgctt gtagtaaaat ctctacttct ttttgtgctt tttgcactcc ctcttggtag
     3661 attttttcag ttaattcctg aagttttcct tgcatattcg attagttttg aaataaaata
     3721 ttcgtttatt gaacaccacg aagatactta tttttatttt tgaaaaaaaa ataaacgcaa
     3781 aaaaatatcc taaaattctt atatttagga tatttttcaa tttttattac attttgcaac
     3841 gctaacaaat caaaatttca cagcaaaatt cacatatgga actcctatta gtaaggattg
     3901 tgcaatatcc caattattta taaaagccaa atcgacagcg atattctctc caaaaaaccg
     3961 aaccccatac gaaaatatag gttcgtagga agaatctgta ggaataagcc aattttcagt
     4021 aaccaaggct gtttttttag caacgcgagt cattcctgat actgttatca aaggtttgtt
     4081 aaacgcattc ctgtcaattg aaccccaagc cacagtagca gttacgttgg tgtttttgtt
     4141 accataggtg cccaaaccgt atagtaatcc taatccagtg ttgctagata aactaaattg
     4201 aggaatactt gcaagaagaa ctcctcctcc aatactcatt ttttcggcaa caggaactcc
     4261 aatttttggt gtaaaataca taataggatt aaattgccct tgacttaaac taataaatgt
     4321 agataaaaat tcgattccac caccgataga aaaataatct gtaataccaa cattgaatga
     4381 attaagtagg agcatggtat tttgataata accttcgcct ttttttaagt taaatgccga
     4441 tgtaccaaat aaataacgtg tcgcatttgg attttcaaac caatattcgt tgtttttgat
     4501 agtttttgta ggtattattt tttttcttga atttgattga taggtatttc tgtacgtgtg
     4561 ccagtagatg cattgtacaa aacaatgtaa tttgtacttt cgctttcgat tgtgcctaga
     4621 tagtgcgttt tatcctttaa agtaacatag tatatggctg tttcttcagt attattttga
     4681 gcatacaaag aaacaaagga aatcgacaaa acgaagaaaa ataggtgtaa ttttttcata
     4741 gttatatttt tactaaaatt aatgttcaac aattcgataa tgaatttgtt tatttggaac
     4801 atcatatacc tccattacag caccgattgg aggtatattg tttttgataa cgtaatgatg
     4861 tagggcagga taaactctac atattcctat aaaaattgac aattgtcctt tgtatggaaa
     4921 agtcgcagtt aaatattttt ttgctggtat ttctttgatc aaaaattttg acgatagttg
     4981 tacaatttta gcactatctg tgaggttgag aatacagcca acatcggagc gtaagttttc
     5041 tttttgtacc tcttgtgggt tatcgtaata aataccaaaa cctttttgag tttcaatgcc
     5101 atactcttca attagtgttt gatatatggt attcattaca tcggctgatt gtttgtaatc
     5161 tcctttgaaa gattggtata caagaatttc tttttgagta gcttgattgt caatcttaat
     5221 agaagaaaac cctccataat aactatatat tattactact atagcaacaa gggctaataa
     5281 aataagcagt gttttttttt gatatttcag tcatttatat atacgtatga acaaaagtag
     5341 caataatttt tacataatag caatttgaaa cctaaaaatt gaaacaagaa agagcgaaaa
     5401 actataatct tagtttttcg ctctttcttt atttgagtga atacagtata ttacaccaat
     5461 gttttaatta acgtttctct gtcgccatac aaataatcaa gaggagcaat ctcaggcatt
     5521 gtataagcac caggagcttg ttttgtaacc gcacgagcac aagaaattaa tatttgagca
     5581 gttaacgctg gattattaat ttgcatgcta aattggaaca attggttttg ggttttaccc
     5641 gaaacccctt tgcgttcaat taaaacgcca tgacccatgt cttgcaaatt atcaacacta
     5701 gcaacttcca taacgtgagt ttcatcttta ctgaaatacg agtcagtttt aatagcttct
     5761 gtaacttttt ctagtttagc accattttct aattccacat acaccatacg gcgatgaacg
     5821 cctgtacccg tagggatagt cattgataga gcatttttta ctccttcttt ggaacgtgct
     5881 actacagagt gtcccatact catacctgga ccaaagttgg tataggtaat accttttgga
     5941 gccatagcta acaatagtgt tcggattact gagtcgctac ctggatccca gccagctgaa
     6001 ataatagagg ctgcatttgt tttttttcca gcaacatcaa gcgattggcg tagtttccaa
     6061 agttcctctc cgtgaatgtc aaagctatct actgtacgaa tgcctttttt tactaattct
     6121 accgctgttt caggaatgct tcgggtagga ccacataaaa tagcaacatc taccttgcct
     6181 aattcagcaa tattcgatac aattttaata ccatttagtt cgtttggtac atttcctacc
     6241 gatgaagcac gtcttactac gcccattaat tccatatcag gagccgcttg tacagcttct
     6301 aatgagaaac gaccaatgtt tccgtagcct acaatggcaa cttttatttt gttcataact
     6361 tagttttttt taattttcag attcttttaa atattcgtcc atagcagcgg cggcttttct
     6421 accatctccc atggctaaaa taacggttgc tccacctctc acaatatctc cacctgcaaa
     6481 aatagctggt atattggttt gcatggaatt ttcgtttaca attacagttc ctttacgaga
     6541 tacttctaat ccttgaattg attgaggtac aagtgggttt ggcgaaacac ccacactcac
     6601 tattaccata tcaacttcaa ttaattcggt ggcaccttcg atggcaatag gcgagcgacg
     6661 accagaagca tctggctctc ccaattgcat ttttcgtagc aacatgtgtg ttactcttcc
     6721 tctttctttg ccatgatact ctataggatt gtgaagtgtc aaaaactcaa tgccttcttc
     6781 tttggcatgt ttaatttctt cgatacgagc aggcatttct tcttccgaac ggcgatatac
     6841 aatcattgct ctatcagcac ctaatctgcg ggcagttcta acagcatcca tagctgtatt
     6901 tcctccacca ataatggcaa cacttttgcc tttatacaca ggcgtgtctg attcggggtt
     6961 agcggcatcc atcaagttaa cacgtgtaag gtattcgttt gctgacatta cgccaattag
     7021 attttctcct ttaatattca taaagttggg caacccagca ccgctaccta caaacattcc
     7081 tttgaacccc tcttcagata gctcatcgaa tgtgatggtt tttcctacga tacaattggt
     7141 taagaattta acgcccattt gtctaagaca ctcaatttca acatccacaa ttttattggg
     7201 taaacgaaat tcaggaatac catattttaa tactccacca atttggtgta gtgcttcgaa
     7261 tacggttaca tcatatccac gttgagccat atcgccagca aaagaaagtc ctgctggtcc
     7321 agagccaaca gcagctactt taatgccatt tttatgagga atttcaggta cagatatctg
     7381 tccactttct ctttcaaaat cggctgcaaa acgctccaag taaccaattg caacagctgg
     7441 tttacccatt tttatgtgaa tacattggct ttcgcattgc aactcttgtg ggcatacacg
     7501 tccgcaaaca gcaggtaatg ccgatgtttc ttttagcgtt tttgctgctt ctaaaaattc
     7561 gccccgttca atgtttttga caaattttgg tatgttaatg cttacaggac acccattcat
     7621 acacgttggg ttggcacagt caagacagcg tttagcttca agcaatgctt gttcaacggt
     7681 taacccaagg tttacttctt ctgagtttct acttctaata gtagcatcta actcattcat
     7741 atgaacgcgt tcaatattcg tacgttcttt gtttttcatg ctagcacgta attcttctct
     7801 ccacgcttcg cttctatcta ctaatgccat gctgattatt ttttaaattt gtcattgatt
     7861 ctgtttcttc atctttgtat gcattcaaac gcataagcat ttcgtcaaaa tctacttgat
     7921 gtgcatcaaa ttctggtcca tcaacgcata caaatttggt tttaccacct actgttacgc
     7981 gacaagcccc acacatccct gttccatcaa ccataatagt atttaatgaa cataatgttg
     8041 gtacctcata tttttttgtt aataaagata caaacttcat catcaccgct ggaccaatgg
     8101 taacacataa atctactttt tcgcgattaa taatattctc tactccattg gtaactaaac
     8161 ctttgtttcc atacgaaccg tcatcggtca taatcacaat ttcgtcagaa tgtttacgca
     8221 attgatcttc taaaataatt aagtcttttg tacgtgcagc taaaactgta attactcggt
     8281 ttcctgcttt ttttaatgct tctacgatag gtagaagtgg ggcaagacct acaccaccac
     8341 cagcacatac aactgtgcca aatttttcga tatgagttgc tttgcctaat ggtcctacaa
     8401 ggtctgttat ataatcacct tcgttaagat tacatatttt ggtagatgaa actcccatct
     8461 tttgtattac caatgttatt gtcccttttt ctaaatcaga cgatgtgatt gttaacggaa
     8521 tgcgttcccc tttttctccc actttgacta gtacaaaatg tccagcgcga cgtgatttag
     8581 ctatcatcgg tgcttcaact tctaacttta ctacattggc agagaagtgt tctttactaa
     8641 ttattttatt cattctataa aaattaaaag attttaaaca gcaaaacatt gcacattacc
     8701 aatatcttga atgtcgacaa cgtgcaatga aatatataaa caagtatgtg tactctatac
     8761 aataccttga gccatcatag ctttagctac tttcataaag cctgctatat ttgctccttt
     8821 cacataatta acaaaaccgt ctttttctgt tccgtatttt acacagtttt cgtgaatgtt
     8881 gttcataata cctttcaaac gggcatcaac ttcttctctc gaccagctca atccgatagc
     8941 attttgagac atttctaaac cagaagtagc cactcctcct gcattggctg cttttccagg
     9001 tccataaaga attttgtttt tcaagaatat ctcaatcgct tctggagttg aaggcatatt
     9061 tgctccttct gatacagcaa tacaaccgtt tgccataaga gtttttgcat cgtcgccatt
     9121 caattcgttt tgagtagctg atggtaaagc gatatcacct ttttcagccc atacacgacc
     9181 accttctacg tatttacaat tgtatttgtc ggcatattct ttaatacgtc cacgttgtac
     9241 tgtttttaaa tccattacgt attctaattt ttcacgtgta ataccttctg gatcgtagat
     9301 ataaccgttt gaatcagata gtgttacagg aattgctccc aattcaatta atttttcaca
     9361 agtatattga gctacgttac ccgaaccaga tactaaacat tttttacctt taatatcaat
     9421 gttgcgtgta gctaacatat ttaataagaa gtaaatattt ccgtaaccag ttgcttctgg
     9481 acgaattaat gaaccaccaa actcaattcc tttacctgta aaagtgccag taaattcgtg
     9541 agtaagtttt ttgtacatac caaacatata agcaacctcg cgtccaccta cacctatgtc
     9601 accagcaggt acgtcagtgt gaggaccaat atgacgatga agttccaata aataagcttg
     9661 acaaaaacgc ataacctcgc cagatgattt tcctcttggg tggaaatcag atccaccttt
     9721 accaccaccc attggtagtg ttgtaagtgc atttttgaac gtttgctcaa acgctaaaaa
     9781 cttcaaaatc gatggattta cagaagagtg gaaacggata ccacctttgt aaggaccaat
     9841 agccatgttg tgttgtacgc gatatgccat gtttacttga acattgcctt tgtcgtcaac
     9901 ccacgatact ctaaaagtaa aaattctatc tggaatacat aacctttcaa ttaagtttgc
     9961 tttttcaaac tctggatgtt ggttatacac ttcttcaata gactcaagca cttcttctac
    10021 tgcctgtaaa tattcaggct catttggaaa tctctttttg agattttcaa ttacgttttt
    10081 tgcgttcata aattggttta ttataaaaaa ttttctactc ggacgcaaaa ttacacattt
    10141 tatccttttt agaaaaaaat aaatattgtt tttgatattg aatatttacg tgtttacatt
    10201 atctgtttat ttggtatatt atactttcat ttataaactg aaaacacgga ttgaatattg
    10261 aatagtaata aaacttagcg tttaaagaaa ttaattgttg aaaaaagtaa tcttttgagc
    10321 aattataact gataacccat acatttatat tcattttcgc aagaattttt ataactttgc
    10381 aacttcaaaa aaataataaa cattattctc aaatttatat ggaattagcc actaagtata
    10441 atcccactga tatcgagtct aaatggtatc aatattggat tgaccacgct ttatttgcat
    10501 caaagcctaa tgataaagag ccttatacta ttgtgatacc accacccaat gtaacaggtg
    10561 tattgcacat gggacatatt ttaaataatt ctattcaaga tattttaata agaagggcac
    10621 gcatgcaagg caaaaatgct ttgtgggtgc cgggcaccga ccatgcttct atcgctactg
    10681 aagcaaaagt tgtaaataaa ctggctgaag aagggataaa aaaaactgat ttaacacgcg
    10741 atgagttttt gaaacacgca tgggattgga cagaaaaaca cgg
//
LOCUS       DAOSRP010000061        10743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6005_length_10743_cov_5.602171, whole genome shotgun sequence.
ACCESSION   DAOSRP010000061 DAOSRP010000000
VERSION     DAOSRP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6005_length_10743_cov_5.602171"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..723
                     /locus_tag="PLJ82_05040"
     CDS             76..723
                     /locus_tag="PLJ82_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOR40905.1"
                     /translation="MQNRPQTPHEERLNGISHLVGIIWSICIIPYCMYIAYDANDLMP
                     FLSVAIFAIGMLAVYTSSTLYHITTDPKRKMILKKFDHISIYFLIAGTYAPFIAHFVP
                     KNTAIIFLIIMWSIVAIGIVYKLFFILRFKWFSLFLYLALGWMIVFVAKPLQENMPLS
                     VFLWIVIGGIFYTAGVYFYVKSAKPYFHSIWHIFVLAGTIAHTIGVYVTLTHSLI"
     gene            complement(715..1089)
                     /locus_tag="PLJ82_05045"
     CDS             complement(715..1089)
                     /locus_tag="PLJ82_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR40906.1"
                     /translation="MKFLCALRDLQRAMVLYENSFLNAYGISLNEGMLLCCLSDKQLK
                     ATELAESIELTCSNCSKVIKLAEDKGYIARKIGKEDKRQMLFTLTESGKQKIVEIETN
                     TIALPEVLQDYIKCYENKPNLN"
     gene            complement(1099..3570)
                     /locus_tag="PLJ82_05050"
     CDS             complement(1099..3570)
                     /locus_tag="PLJ82_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR40907.1"
                     /translation="MKHIIVGGVAGGATAAARIRRIDEHAEIILFEKGNDISYANCGL
                     PYYIGGVIDDRDKLFLQTPASFGQRFVVDVRVRSEVIGVDTANKTVTVRTNKNETYTE
                     TYDKLLLSPGAYPFVPPMQGIDLDGIFTLRNVADTDKIKSYIQSNEIKKAVVIGAGFI
                     GLEMAENLHHIGAEVSIVEMANQVMTPIDFAMAAMVHQHLNQQGVNLYLQETVLSFKK
                     KGNGLDVQFKSGKVLYADMVILSIGVRPETTLAKQAGIKLGEVGGIWVDEYLQTSAKD
                     VYAVGDAIETPHPITNQSWLNYLAVPANRQGRIVADNMVFGNKEVYEGAIGTSIAKIF
                     DITVAATGLNENRLALQKVDYLTSFTHSGSNAGYYPGAMPMSIKLNFAPKTGKLLGAQ
                     IVGYVGVDKRLDQIALLIKQGGTIYDLQKIEHAYAPPFASAKDPIAIAGYTAQNVLTG
                     KMPIVHWQTVRDMDSSKIFLLDVRTEDEFQLGSLTGAVNIPLDVLRNHLDELPQDKPI
                     YVFCAIGLRGYLATTILLQRGYKQVYNISGGYKTYETAVSKIVNPSELSQPEKRAYEL
                     DLDIQTPKPAKKYLAVDACGLQCPGPILKMKKSMDDLVEGDRIEMVATDAGFTRDAQA
                     WCNMTGNTLVSQSAKEGKYTVIIEKGNSVIANESTECCAPSSSGKGKTLIVFSDDLDK
                     ALAAFVLANGAAATGEKVSIFFTFWGLNTIKKSKKPAVKKDIFGKMFGLMLPASSQKL
                     NLSKMSMFGMGKYMMRFIMKLRGIDTLESLRQQALENGVEFIACQMSMDVMGVKREEL
                     LDEVNIGGVATYMERADKSNINLFI"
     gene            complement(3720..4442)
                     /locus_tag="PLJ82_05055"
                     /pseudo
     CDS             complement(3720..4442)
                     /locus_tag="PLJ82_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993569.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(4783..6606)
                     /locus_tag="PLJ82_05060"
     CDS             complement(4783..6606)
                     /locus_tag="PLJ82_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOR40908.1"
                     /translation="MNTLDNQQGTEESLSKSIEPADKEVSITTLSTEGEPTSVGHTNH
                     EESTFVSLEDLLTALELAIKMPNAKNEITRIKAEFYKQLAINNAKEKAAFDAKHIDEG
                     LIFEPKIGVEEERFKQLLATYKEKRNQEIQQQEQSKEDGLLKKESLLARLQMVIESTD
                     DVSKSFPVVQEIQQEWRSIGAIASEKYKPLVRKYQLLMEQFYDFVKISNDLRDYDFKK
                     NLEAKTELCEQAEVLLNDADINKAFNRLQQLHAEWRELGPVSYEKREEIWTRFKTASD
                     SINKNHASYYQQRRDEEEKNLTLKQALCSQVSAIQVGEFSSYKQWDEGTKQVLALQEE
                     WKHIGFAPKKHNAKIYEQFRTLCDDFFVKKSEFYKKSKSVLQDNLEKKKALCARAEAL
                     KDSKDWKKTTEELVLLQQEWKTIGAVPKKISNHIWNRFLVACDYFFEQKEKEFKGKKT
                     EEHDNLEAKKALIEEIKAYKGTSIDDVRTFMDTYATIGHVPFKEKDKIYNAYKEALDA
                     QFTRLKVGVTERKLSMFKQSLSSQTEKSTAGLYREREKLVRAYERLNADIQTRENNFG
                     FLNVTSKKSSGLIQEMEATIEKLKQERDLLLQKIKMLEDNL"
     gene            6890..9319
                     /gene="thrA"
                     /locus_tag="PLJ82_05065"
     CDS             6890..9319
                     /gene="thrA"
                     /locus_tag="PLJ82_05065"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOR40909.1"
                     /translation="MKVLKFGGTSVGSISNIFQIKNIINAIDEPVIIVVSAVGGITDK
                     LIHTANKAANGNKEYLADLQEIISIHHQIIEGVVEDNDKNTVLATVNHFFDELANIYK
                     GVYLIKDLSVKTQNVVVSYGEKISSYFLSFALNATLYNACDFIKTETILGKRAVDYTT
                     TNRLVKELFVNHPNRCIVGGFIASCSKTNKVTNLGRGGSDYTAAILAAALDARKLEIW
                     TNVDGFLTADPRLVPNAYTILEMSYIEAMELSNFGAKVIYPPTIYPVYAKNIPVYIKN
                     TSNPEIKGTKITNKKNSGDKTIKGISSIQDTCLITIQGMGMVGVLGISSRTFKTLADA
                     GINVFLISQASSENSTTFAVKHKDADAAVDVLKREFEKEIEKGSIEKIVPEKNLATIA
                     IVGENMKHNTGIAGKLFNTLGRSGINIIACAQGASETNISFVVSMGNLKKSLNVIHDA
                     FFLSDYQALNIFLVGTGTVGGSLLEQIHQQQSKLLEQNSVKLNVIGITDLNGSIFVPQ
                     GIDLSNYMEQIHQNGKQLNPEQVCDTIIEMNMFNSVFVDCTASPQIANLYEKLLNNNI
                     SVVAANKIAASSDYDHYMHLKSLARKKGIKYLFETNVGAGLPIINTINNLINSGDKIL
                     KIEAVLSGTLNFIFNTISEKISFSQSIQMAKEARYAEPDPRIDLSGKDVIRKLVILAR
                     ESGYKIDQDDVAKNLFVPDSYFQGSVDDFWNTIGELDAEFEEKRKKLAAQDKKYRFVA
                     KMDNGKCSVGLQEVDARHPFYDLEGSNNIIMIHTERYFEYPMIIKGYGAGASVTAAGV
                     FGDIISIANIR"
     gene            9335..9880
                     /locus_tag="PLJ82_05070"
     CDS             9335..9880
                     /locus_tag="PLJ82_05070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR40910.1"
                     /translation="MLVNSKKNWWIMACILLLASCTEPTPQNPTNKLVSTDSVQQNLL
                     LLNQAFVALEQQEINEYIAQHRLNVHLSENGYWYRIDKRGTGNTLKANQLVTIEYYLE
                     LLDGTLCYSSEKNGIKQFRVGKFEVEKGLDDFMLLLSLGAEATCIIPSYLAHGVVGDR
                     NCIPPRTPIIYRIKVISIKDN"
     gene            9942..10481
                     /locus_tag="PLJ82_05075"
     CDS             9942..10481
                     /locus_tag="PLJ82_05075"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOR40911.1"
                     /translation="MKLYNTLLFFSISIVLVFTQCTSEETYAQKLKTERLKFAEYVEK
                     GNIELSSDSIYCFSLPTPWPENLYYRTPRGAYINLIDKGTSKEATEGSTVIVRYVATN
                     IAGDTIANNTSTSVSRDGYEFIYKEGSSVPCIGWNDAMLYLNNNGKAKVIVESKIGFS
                     QHQTSVITVISDVTVSITN"
     gene            10499..>10743
                     /locus_tag="PLJ82_05080"
     CDS             10499..>10743
                     /locus_tag="PLJ82_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR40912.1"
                     /translation="MTLIKSISGIRGTIGGKAGEGLSPLDIVKFTAAYATYILKNTTK
                     KTNKVVVGRDARISGEMVNNIVNGTLMGMGIDVVNIG"
BASE COUNT         3415 a         1925 c         1698 g         3705 t
ORIGIN      
        1 cataatatgg aggaataatc taatcaaata gtagggctaa gctaagtaag tttagccttt
       61 ttttcttatc ttactatgca aaatagacca caaacaccac acgaagaacg attgaatgga
      121 ataagccatt tggttggtat tatttggagt atatgcataa ttccatattg catgtatatt
      181 gcctacgatg ccaatgattt aatgccattc ctttctgttg caatttttgc aattggcatg
      241 ttagcggtat acacttcatc tacgctttac catattacca cagaccctaa acgaaaaatg
      301 atacttaaaa aatttgacca tataagtatt tattttttaa ttgctgggac atacgcccca
      361 ttcatagcac attttgttcc aaaaaataca gcaattattt ttttgattat tatgtggtct
      421 atcgtggcaa ttggtattgt atataaacta ttttttattt tacgttttaa atggttttca
      481 ttgtttttat accttgcttt gggctggatg attgtatttg tagctaaacc gctacaagag
      541 aatatgcctt tgtcagtttt cttgtggatt gtaatcggag gaatttttta tactgctggg
      601 gtatactttt atgtaaaaag tgccaaaccg tattttcata gtatttggca catttttgta
      661 ttagcaggta caatagcaca tacaattggg gtatatgtta cacttaccca ttcactaatt
      721 taagtttggt ttgttttcgt aacatttgat ataatcttgt aagacttctg gtaaagcaat
      781 tgtatttgtt tcaatttcaa ctattttttg ttttccactt tcagtcaacg tgaacagcat
      841 ctgacgctta tcttctttgc caatttttcg tgcaatatac cctttatctt ctgccagttt
      901 aatcactttt gaacaattgg aacaagtaag ttcaatggat tcggctagtt ccgttgcttt
      961 tagttgttta tcagataagc aacataacaa catgccctcg tttaatgaga tgccataagc
     1021 attcaaaaag ctattttcat acaacaccat cgcacgttgc aaatcgcgta aagcacataa
     1081 aaatttcata gtaatatatt aaataaaaag attaatattt gatttatcgg cacgttccat
     1141 ataggtagcc acaccaccaa tatttacttc gtctagtaac tcttctcttt ttacacccat
     1201 cacgtccatc gacatttgac aagcaataaa ctctacacca ttctccaagg cttgttgacg
     1261 taaagactcc aaggtatcaa taccacgtaa tttcattata aaccgcatca tatatttccc
     1321 catgccaaac atactcattt ttgataaatt gagtttttgg gagctagcag gtaacataag
     1381 tccaaacatt ttaccaaaga tatctttttt tacagcaggt ttctttgatt tttttatggt
     1441 atttaatccc caaaaagtaa agaaaatgga tactttctct ccagttgctg cagccccatt
     1501 ggctaataca aaagcagcta aggctttatc taaatcatca ctaaatacaa taagtgtttt
     1561 ccctttacct gatgaagatg gggcacaaca ttctgttgat tcgttggcaa taacactatt
     1621 tcctttttca ataattacgg tatatttccc ctcttttgct gattgtgata caagggtatt
     1681 gccagtcata ttacaccaag cttgtgcatc tcgcgtaaaa cctgcatcgg tagcaaccat
     1741 ttcaatacga tctccttcta ccaaatcatc cattgatttt ttcatcttta agattggacc
     1801 aggacattgt aatccacaag catctacagc taggtatttt ttagcaggtt ttggtgtttg
     1861 tatatctaaa tcaagctcat acgctcgctt ttcgggttga gataattcgc tcggattcac
     1921 aatcttactg acagctgttt cgtacgtttt atagcctcca gaaatgttat aaacttgctt
     1981 atatccacgt tgaagcaata tagtagtcgc taaatacccc cttaatccaa tggcacaaaa
     2041 aacataaatg ggtttatctt gaggcaattc gtcaaggtga ttgcgtaaca catccaaggg
     2101 tatattcaca gctccagtta aactaccaag ttgaaattca tcttcggtac gcacatctag
     2161 caaaaaaatc ttactgctat ccatatcacg tacagtttgc caatgtacta taggcatttt
     2221 tcctgttaat acattttgag cagtatagcc tgcaatggca ataggatctt tagccgaagc
     2281 aaaaggtgga gcgtatgcat gttctatttt ttgtaaatca tatatagtgc ctccttgttt
     2341 tatcaacaga gcaatttggt ctaagcgttt gtcaacacca acgtatccaa ctatctgagc
     2401 tcccaatagt ttgcctgttt taggagcaaa atttaatttt atagacatgg gcattgctcc
     2461 tggataataa ccagcattgg aacccgaatg cgtaaatgaa gttaaataat ctactttttg
     2521 caatgctaag cggttttcat tcaagccagt tgcggcaact gttatatcaa atattttggc
     2581 aattgaggta ccaatagctc cttcatacac ctctttgttg ccaaacacca tgttatctgc
     2641 cacgattctc ccttgacgat tagctggcac agctaaataa tttaaccaag attggttcgt
     2701 aattggatga ggggtttcaa tggcatctcc tactgcatat acatctttag cagaagtttg
     2761 caaatactca tctacccaaa tacctccaac ttcacctaat tttattcctg cctgctttgc
     2821 caaagtcgtt tcgggtctaa ctcctattga caaaattacc atatccgcat acaacacttt
     2881 tccgctctta aattgcacat caagtccatt tccttttttc ttaaaagata aaactgtttc
     2941 ttgtaaatac aaatttactc cctgttggtt taaatgttga tgcaccatag cagccattgc
     3001 aaaatcgata ggagtcatta cttgatttgc catttcaaca atagacacct ccgcaccaat
     3061 atgatgcaaa ttttcagcca tttctaagcc tataaaacct gctccaataa ctacggcttt
     3121 ttttatttcg ttcgactgaa tataactctt aatcttatct gtatcagcca cattacgcaa
     3181 tgtaaatatg ccatctaaat caataccttg catgggagga acaaatggat atgctcctgg
     3241 agataaaagc aacttatcgt atgtttctgt gtacgtttca tttttatttg ttcgtacagt
     3301 aaccgttttg tttgctgtat ccacaccaat tacctcagag cgaacacgta catctacgac
     3361 aaaacgttga ccaaaagacg ctggtgtctg caaaaacaac ttatcccgat catcaattac
     3421 tccgccaata taatatggca atccacaatt tgcatacgat atatcattac ctttttcaaa
     3481 caagataatt tctgcatgtt catcaattct tctaatacgt gctgcagcag ttgctccgcc
     3541 tgctacacct cctacaatta tgtgtttcat ttagcaaagt tagaataagt aaatataatt
     3601 tgacacaaag atatacaata tttttttatg taatctattt ccataagaaa atagtaattt
     3661 tactacaaaa aaaacaccaa cttctttgtt ggtgtttcat tacatataaa catcagattt
     3721 tattcgaacg aacccatttt taacatatta atctccattt gtgataaaaa tcgccattta
     3781 cctcgtgcca atttcttttt tgttaacccg caaaaataaa ccctgtctaa ttttacaact
     3841 ttatatccca gcgattcaaa aatgcgacgc acaatacggt ttctgccaga atgaatttct
     3901 acacctaatt catcattcat atcctctaaa tagtgtaact catctacttc aataagtcca
     3961 tcttctaata ctaacccagc tttaatttta tcaaaatgtt ctctagttag tgctttatct
     4021 aagcctacgt gataaatttt ctttttgttg tatttaggat gtgttaattt agatgctaaa
     4081 tcgccatcat ttgttagcaa caaaactcct gtagtatttc tatccaatct tcctactgga
     4141 tacaccctat cattggtggc acttttaacc aaatcgagta cagttaatct ttcttgtgga
     4201 tcatcagagg tggttacaca attcttcggt ttatttagca agatgtatgt tttcttttct
     4261 ggacgtatta aatcgttatg aaaatagact ttatcttcgt aagaaacttt atatcctaat
     4321 tcagttataa tcacatcatt cactcttaca acacctgcac taatatattc atcagcttca
     4381 cgccttgagc atacaccaga gttagccaaa taacgattta agcgaatttt tccatcatca
     4441 atttttgttg gttgtttttt aatcaacaca cattttcgtt gaggagcttt atctgaatat
     4501 ggtcttgatt cacagcttgt tgaacttgtt cgttgtgatg aaaaacgttt agacggtctt
     4561 ttagtatcct ctttaggttt aaccaaggat tttccaactc tttttttaga ataagaatcc
     4621 gttgtttcat caacggaagc attgctataa tttttaaact ttggggcaaa acgatcaggt
     4681 tttcgttctg catttctatc cgcttttcta tcagtatatt ttttagtgga attggacgtt
     4741 gtcggtcttt ttttttgtgt tctcatgttg tagattagta gcttagagat tgtcttctaa
     4801 cattttaatt ttttgaagta acaaatctct ttcttgtttt aatttttcaa tggttgcttc
     4861 catttcttga ataagtccag atgatttttt agaagttaca tttaaaaaac caaaattgtt
     4921 ttctcttgtt tgaatatcag cgttaagacg ttcataagct cgcaccaatt tttcgcgttc
     4981 acgatacaat ccagcggtag atttttctgt ttgtgaagat agtgattgtt taaacatact
     5041 caactttcgt tctgtaacac ctaccttcaa acgagtaaat tgtgcatcta aagcttcctt
     5101 gtaagcatta tatattttgt ctttttcttt aaaaggcaca tgaccaattg tagcatatgt
     5161 atccataaat gttcttacat catctataga agttccttta tatgctttaa tctcttctat
     5221 taaggctttt ttagcttcaa gattgtcatg ctcttcggtt ttctttcctt taaattcttt
     5281 ttctttttgt tcaaaaaagt aatcacatgc tactaaaaaa cgattccaaa tatgatttga
     5341 tattttttta ggaacagctc caattgtttt ccattcttgt tgcaaaagca ctaactcttc
     5401 ggttgttttt ttccagtctt tgctatcctt caatgcttct gccctggcac acaaggcttt
     5461 ctttttttct aaattatctt gtaatactga ttttgatttt ttatagaatt cagacttttt
     5521 gacaaaaaaa tcatcgcata atgttctaaa ttgttcatat attttggcat tatgcttttt
     5581 aggagcaaat cctatatgtt tccattcttc ttgcaaagct agcacttgct tagttccctc
     5641 atcccattgc ttatagctag aaaattcacc cacctgaata gctgaaactt ggctacataa
     5701 agcttgttta agtgttaaat tcttttcttc ttcgtctcta cgttgttgat aataagaagc
     5761 gtgatttttg ttaatagaat ccgatgcagt tttaaatctt gtccatatct cttctcgttt
     5821 ttcatacgat acaggtccta attctctcca ctctgcgtgc aattgttgca aacgattaaa
     5881 agccttattt atatccgcat cattcagtaa tacttctgct tgttcgcaca attcagtttt
     5941 tgcttcaaga tttttcttaa aatcatagtc gcgtaaatca ttactgattt taacaaaatc
     6001 ataaaattgc tccatcaaca attgatattt tctaaccaaa ggtttgtatt tttcagaagc
     6061 tattgcacca atagaacgcc attcttgctg tatttcttgc accactggaa atgatttact
     6121 tacatcatct gtactctcaa tcaccatttg taatcttgct agtaacgatt cttttttaag
     6181 caaaccatcc tctttggatt gttcttgttg ctgaatttct tgatttctct tttccttgta
     6241 tgtagccaat aattgcttaa aacgttcttc ctctacacct attttaggtt caaaaattaa
     6301 cccttcatcg atatgctttg catcaaacgc tgctttttct ttagcattat ttatcgctag
     6361 ttgtttgtaa aactctgctt taatgcgagt tatttcattt ttagcgtttg gcattttaat
     6421 tgccaattct aatgctgtta aaagatcctc caaactaaca aatgtggact cttcgtgatt
     6481 agtatgtcct actgaagttg gctctccttc tgttgataga gtagttatag atacctcctt
     6541 atcagcaggt tcaatgcttt ttgataagga ctcttctgtt ccttgttggt tgtcaagtgt
     6601 gttcatagca aatatggaac taaatcaaaa ataaaattta caatcgacaa agatacaagt
     6661 ttttttttag agacttcatt ttttctggac ataatcaata aaagcacaaa aaattgggtt
     6721 atatatttca gtaatcataa aaaaatgagt aatattgcaa gttaatttag aaaagacata
     6781 ataaaacaca atctattggc acaataagta catctactca gcatacaaac agagtctttg
     6841 ttacattatt ttattcggta gtgcgtaaaa taaacacaat agtaaaaata tgaaagtatt
     6901 aaaatttgga ggaacatccg taggttccat ttcaaacatt tttcaaatta aaaatatcat
     6961 caatgccatt gatgagccag ttatcattgt agtttctgca gtaggaggca ttacggataa
     7021 gttaatacat acagccaata aagcagcgaa tggaaacaaa gaataccttg ctgatttgca
     7081 agaaattatc agcatacatc atcaaattat tgagggagta gttgaggata atgataaaaa
     7141 cacagtttta gctacagtca atcacttttt tgacgaatta gcaaatattt ataaaggagt
     7201 gtatttaatc aaagatttat ctgtaaaaac tcaaaatgta gttgttagtt atggagaaaa
     7261 aatctcatcc tactttttaa gttttgcttt aaatgctact ttatataatg cctgcgattt
     7321 tattaaaacg gagacaatct tgggtaaaag agctgttgat tataccacta ccaacagatt
     7381 ggtaaaagaa cttttcgtaa accatccaaa cagatgtatt gttggtggat ttattgcttc
     7441 gtgtagcaaa acgaataaag taaccaactt aggcagaggc ggttctgact atactgctgc
     7501 aattttagca gcggctttag atgctcgcaa attagaaata tggacaaatg tagatggttt
     7561 tcttactgcc gaccctagat tagtgccaaa tgcttatacc attttggaga tgagttatat
     7621 cgaagctatg gagttgtcca attttggagc taaagtaata tatcctccta caatctatcc
     7681 tgtatatgca aaaaacatac ctgtatatat taaaaataca agcaatcctg aaataaaagg
     7741 cacaaaaatt actaataaga aaaactctgg agataaaact ataaagggta tttcatctat
     7801 acaagacacc tgcttaatta ccatacaggg tatgggtatg gttggtgtat tgggcataag
     7861 ttcacgcact tttaaaacac tagcggatgc tggaattaat gtatttttga tatcacaagc
     7921 ctcgtcggag aactctacaa cttttgcagt aaaacataaa gatgcagatg ctgctgttga
     7981 tgtattaaaa cgagaatttg aaaaagagat agaaaaaggt agtattgaaa aaattgttcc
     8041 tgaaaaaaac ttagcaacca tagctattgt tggtgaaaat atgaaacaca acacgggtat
     8101 agctggcaag ctattcaaca cccttggtag aagtggcatt aacatcattg cgtgtgctca
     8161 aggtgcatca gaaacaaata tttcgtttgt tgtgagtatg ggtaatttga aaaaatcgct
     8221 caatgttatt cacgatgcat tctttttgtc tgattatcaa gctctcaata tatttttagt
     8281 aggaacagga actgtgggtg gtagtttact cgaacaaata caccaacaac aaagtaaact
     8341 tttagaacaa aatagtgtaa aactaaatgt gattggaata accgatttaa atggttcaat
     8401 atttgttcca caaggaattg atttatcaaa ctatatggaa cagattcatc aaaacggaaa
     8461 acaattaaat cctgaacaag tatgcgatac catcattgaa atgaatatgt ttaattctgt
     8521 ttttgttgat tgtactgcta gtccacaaat tgcaaaccta tacgaaaaac ttcttaataa
     8581 taacatctct gttgtggctg ccaataaaat cgcggcatca tccgactatg atcattatat
     8641 gcacttaaaa tctttggctc gcaaaaaagg aattaaatac ctatttgaaa ccaatgttgg
     8701 tgctggatta cccattatta acactatcaa caatttaatt aatagtgggg ataaaatatt
     8761 aaaaattgag gctgtacttt ctggcacatt aaattttatt ttcaatacta taagcgaaaa
     8821 gatatctttc tctcaatcta tccaaatggc aaaagaggct cgctatgctg aacctgatcc
     8881 ccgcattgat ttaagcggaa aagatgtaat tcgtaaattg gtaatcttag ctcgcgagtc
     8941 aggctataaa atagatcaag atgatgtggc aaaaaatttg tttgttcctg attcgtattt
     9001 tcagggttca gttgatgatt tttggaatac tattggcgaa ttagatgctg aatttgaaga
     9061 aaagcgtaaa aaattagctg ctcaagacaa aaaataccgc tttgtagcaa aaatggataa
     9121 tggaaaatgc tcagtaggtt tgcaagaagt ggatgcacga cacccatttt acgatttaga
     9181 agggagcaac aatatcatta tgatacatac tgaacgttat tttgaatatc caatgatcat
     9241 taaaggatat ggtgctggtg ctagtgttac agcagctggc gtattcgggg atattattag
     9301 tattgctaat attcgataag aaaaaacatc tctaatgttg gttaatagta aaaaaaactg
     9361 gtggataatg gcatgtatac tgcttcttgc tagttgtact gagcctactc cacaaaaccc
     9421 aaccaataag ctagtatcaa cagatagtgt gcaacaaaac ttattacttt taaatcaagc
     9481 ctttgtggct cttgaacaac aagaaataaa cgaatacatt gctcaacata ggttaaacgt
     9541 acatttgtca gaaaatggct actggtatcg tatagataaa cgaggtacag gaaatacgct
     9601 taaagccaat caattagtaa ctattgaata ttaccttgaa ctacttgatg gtaccctttg
     9661 ttattcttca gaaaagaacg gtataaaaca atttagggtg ggtaaatttg aagtagaaaa
     9721 aggattagat gatttcatgt tattactctc attgggagcc gaagctacat gtatcattcc
     9781 ttcgtactta gctcatggtg tggttggaga cagaaattgt atacctcctc gtacaccaat
     9841 tatatataga atcaaagtaa taagtattaa agataattaa tactcaatca taacacaatt
     9901 tttatgtgta cttttgcgta cgtttattta aatatcattt tatgaagtta tacaacacac
     9961 ttttattttt ctctattagc atagtacttg tttttacaca atgtacttcg gaagaaacat
    10021 atgcacaaaa acttaaaaca gaacgcttaa aatttgccga atatgtagaa aaaggaaata
    10081 ttgagttatc atctgattct atttattgtt tttctttacc caccccatgg cccgaaaatt
    10141 tatactatag aacacctcga ggggcatata ttaacctcat agataagggg acttcaaaag
    10201 aagcgacaga aggttcaacc gtaattgtaa gatacgtagc aaccaatata gctggagaca
    10261 caattgcaaa taacacctct acatctgtaa gtagagatgg atatgaattt atttataaag
    10321 aaggaagtag tgtaccttgt ataggatgga atgacgctat gttgtatctg aataacaatg
    10381 gtaaagcgaa agttattgtt gaatctaaaa ttggattttc gcaacaccaa acttctgtta
    10441 ttacagtaat ttctgacgtg actgtatcta ttacaaatta atatacaaac acagaattat
    10501 gacattaata aaatctattt caggtattag aggtaccata ggaggaaaag ctggtgaagg
    10561 gttgagtcca ttggatatag taaaatttac agctgcttat gccacataca tccttaaaaa
    10621 cactacaaaa aaaaccaata aagtggtagt aggtagagat gctcgtattt cgggtgaaat
    10681 ggttaataat attgtaaatg gcacattaat gggtatgggt atcgatgtag taaatatagg
    10741 att
//
LOCUS       DAOSRP010000062        10666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6057_length_10666_cov_4.593158, whole genome shotgun sequence.
ACCESSION   DAOSRP010000062 DAOSRP010000000
VERSION     DAOSRP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_10666_cov_4.593158"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLJ82_05085"
     CDS             <1..386
                     /locus_tag="PLJ82_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOR40913.1"
                     /translation="GANVDKAEMAKTQYKTALSLFPMHESGWKPQVVTCSAFYKTNID
                     AIWTMLMQYIQFTQQSGYFETKRHQQASYWMYESINQELRSSFYAHSEVNALLKEMEV
                     KVTNGEISSFIAAKRLLDVYDKIRK"
     gene            405..1019
                     /locus_tag="PLJ82_05090"
     CDS             405..1019
                     /locus_tag="PLJ82_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOR40914.1"
                     /translation="MKSFGNYIFILVMLVACSQYKSKSILQGTITDSRYKKVYLLQQI
                     TPTSWEMLDSTSIVDNHFMFADLPSEYNQLFVQLDNTIPFVVFVDKGTIELAIPAQNV
                     HETKVKGSSLHDEYMMLQDSIDFLLHQRLLFYKDAIVAQNDGRQTDATILQQKYIHSK
                     NNVFHFLQKQKGANPTSAPLLYIIKTYKPYFTDTEYRTLSASLE"
     gene            complement(1021..1500)
                     /locus_tag="PLJ82_05095"
     CDS             complement(1021..1500)
                     /locus_tag="PLJ82_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOR40915.1"
                     /translation="MAQYQLDSLDEKILQLISKNARTPFLEVARECNVSGAAIHQRVQ
                     KLISVGVIKGSEFIIDPVKIGYNTCAYMGIFLKDANFFKEVVDELLKIPEVVECHYTT
                     GKYAMFIKIYARNNSDFLSIIHDKLQSIKGIASTETLMSLGETFKRQITVEGVADKE"
     gene            1749..2450
                     /locus_tag="PLJ82_05100"
     CDS             1749..2450
                     /locus_tag="PLJ82_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOR40916.1"
                     /translation="MATKRLKEIKPIEETADIWTLLSSDQAHYLQTNLQVISAKKNEV
                     IYSDGEIPSHLFCLVSGKVKVYKQGIGGRNQIVRVIKPNELFGYRAMFALEAYVTCAS
                     AIEPCVIYAIPEDVIVSIIKNNAQLAFAFIQFLAVDLGISDSRTVTLTQKHIRGRLAE
                     SLLFLRDNYGVESDGITLSIYLSREDLASLSNMTTSNAIRTLSLFSNEGMIVVEGRKI
                     KIVDVESLVKISKFG"
     gene            complement(2452..4191)
                     /locus_tag="PLJ82_05105"
     CDS             complement(2452..4191)
                     /locus_tag="PLJ82_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOR40917.1"
                     /translation="MILFKKLLLSFVGIFVFFAAIIFIINVQYEKHMVKARYTETLQF
                     YTEIIDLNTKTARKTEIPNALVDELQKKDVYFSIYNTSGECIYSTPMNDSVIYNVSER
                     AFGMITEKNTPEHTLVVRYDQTKSSYFFYGKKYDTHTILASIPYEYIISDVWKIRNFT
                     ASFFMFFVLSLSSLAFYFVSNKLLHTVKNLKKVILKMENKEDFDDDLLFLNKEFKEIS
                     SFIVNTYKNLTATKDALSLEKEKLFKHLQISQEGLAIYTFDRKEILANENFIQYINLI
                     SNKKISTSDSIFSLAEFSPITSFLNENQYNQNNPNEVTDKIVEIDKNDYTLLVRCIIF
                     QDKTFEISINDISKQVQNNLIKKQLTQNISHELKTPVSSIQGFMETLIENPDMEQEKK
                     DFFIQRCHAQAVRLSYLLRDISLLNKLDEGSYTFEKEKIVLNDIVEGVLKDVSLELEQ
                     KNIRVEVFLKHNMTIMGNYSLLYSIFRNFIDNSLHYAGNDISIIINCYRTDKDFYYFS
                     YADTGVGVSENHLTRIFDRFYRIDKGRSRKMGGTGLGLAIVKNAIAFHQGRISAKPHE
                     TGGLEFVFTLRKE"
     gene            complement(4207..4905)
                     /locus_tag="PLJ82_05110"
     CDS             complement(4207..4905)
                     /locus_tag="PLJ82_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR40918.1"
                     /translation="MSTFRILVVDDEEDLCDILQYNLEKDGYIVDIAYSAEDALKKDL
                     TQYNLFLLDVMMGAMSGFKLGSMLKKDETTSHIPIIFITAKDTETDTLTGFTIGADDY
                     ITKPYSVKEVVARVRVVLKRTTEQKDGTPMSKDKILRFEQLFVNLTTKTTLIDKQEIN
                     LTKKEFEILSLLMSQPNNVFSREDILQQIWNNADVTDRTIDVNITRLRKKIEPYDKNV
                     VTRLGYGYCFETSN"
     gene            5176..5943
                     /locus_tag="PLJ82_05115"
     CDS             5176..5943
                     /locus_tag="PLJ82_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR40919.1"
                     /translation="MNRKYRLGLVLSGGGAKGFAHLGVLKAIEEKGLKPDIIAGVSSG
                     ALMGLLYADGKSLDEIMKFFEKASLFDSISPTLPKLGLMNPSKFKRQLRSFLTAKSFE
                     ELAIPFVVNATNLTDGKMTVFRTGESLVEAVIGSSSVPLLFTPQIIEGKQYVDGGVFC
                     NMPVSPIRDECDYIIGVHVNPICTETKAESIIEIAERVLHYAIQSSTINEKKLCDVVI
                     DVKEVKKYGMFNLDKKQEIFDYGYTKAKEVLENFYIE"
     gene            complement(5946..6479)
                     /locus_tag="PLJ82_05120"
     CDS             complement(5946..6479)
                     /locus_tag="PLJ82_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR40920.1"
                     /translation="MISLEFISHNNHPLLPSIQSIYEEAFPPEEKVDFSSLLSHKFTD
                     DCAFKLYAILDKNETCGMLSIVEYTFFVFIYYLAITPAKQGLGIGNKVLELIKKESTT
                     PLILEVEHATQDISKRRIEFYVRNGFHVNQYPYFQPALHQHTDAVPMHIMSYPHALSE
                     EDFLTVKTALYEHVYCV"
     gene            complement(6572..>7120)
                     /locus_tag="PLJ82_05125"
     CDS             complement(6572..>7120)
                     /locus_tag="PLJ82_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40921.1"
                     /translation="KGRFFLSNTPDSISKLPTSTFNRMVVWVQLEDKQTQKVFFLFST
                     HFDHPTTQKGIDTRKQQATIAREQILALTEGYPVIFTGDFNCEPTEPAYTILSGEWHD
                     AYETTPTLRQGGYINKEHTYTGLYSSNDKVSKRIDYIFINDKVHVNSYIAADEKLAHT
                     TYPSDHLPILIECSIQDAVDTE"
     assembly_gap    7121..7220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7221..7811)
                     /locus_tag="PLJ82_05130"
     CDS             complement(<7221..7811)
                     /locus_tag="PLJ82_05130"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40922.1"
                     /translation="MKTWNYLLLTAVLLLTACSAKEKEPEIIKYTVTIEQPEGGTITV
                     KQGNTVIENGTEIIKGTSLTVTATPDEFYTLESVNVSSPFELTQDTTITAVFEPMVMY
                     TVTIEQNEQGTITVLNKGNVVNSGDQVPENTELSVIAFGNNSHVFSNINTTPYLVLTQ
                     DTTITASFEAIDEQEKYIIGSYNIRYYTTGDTGGKFW"
     gene            complement(7844..9736)
                     /locus_tag="PLJ82_05135"
     CDS             complement(7844..9736)
                     /locus_tag="PLJ82_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOR40923.1"
                     /translation="MAVINYIQLENVSKRFGDLLLFEGLNMDISEGQRVALIAKNGTG
                     KSTLLNIIMGKESLDEGKITFRRDITLSFLEQDPSYNPEQTILDTFFSSNNETIRLIA
                     QYEAYISKNENLDSPEFQELVEQMEIRKAWDYDIKIKQILAQLKITDFDKKIKHLSGG
                     QLKRVALANALITEPDLLILDEPTNHLDLEMTEWLEEYLIRSGLTLLMVTHDRYFLDR
                     VCTDIIEIDQKQLYAYKGNYSYYLEKRQDRIDAQNAELARANNLFRKELDWMRRQPQA
                     RATKAKSRIDSFYDLEKKVAQKRQDESVKLQMNATYLGSKIFEAKYISKQFDDFVLIK
                     DFYYNFSRYEKMGIIGKNGTGKSTFLKLLLGIIKPDSGSFDIGETVSFGYYSQDGLKF
                     DEQMKVIDVVRDIAEEINIGNGKKLSASQFLQHFLFTPETQHNYVYKLSGGEKRRLYL
                     CTVLMKNPNFLVLDEPTNDLDIVTLNVLEEYLANFKGCVIVVSHDRYFTDKVVDHLLV
                     FQGNAVIKDFPGNYSDYREWKEQQNAIDAKQAEIKQKNKVEEPKRSVNRTRKLSFAEQ
                     REYSLLESDIENLETEKKDLETMLSGGSIPQQELIKASERIAEVIELIDDKTMRWLEL
                     SEYTNN"
     gene            9883..>10666
                     /locus_tag="PLJ82_05140"
     CDS             9883..>10666
                     /locus_tag="PLJ82_05140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR40924.1"
                     /translation="MAKELKDLTKRSENYSQWYNDLVVKADLAEQSAVRGCMVIKPYG
                     YAIWEKMQQELDRMFKETGHVNAYFPLLIPKSFLSKEAEHVEGFAKECAVVTHYRLKN
                     AEDGSGVVVDPAAKLEEELIIRPTSETIIWNTYRNWIQSYRDLPILCNQWANVFRWEM
                     RTRMFLRTAEFLWQEGHTAHATKEEAEEEALKMLHVYGDFAENFMAVPVVKGIKTANE
                     RFAGALETYTIEAMMQDGKALQSGTSHFLGQNFAKAFDVQFIT"
BASE COUNT         3224 a         1700 c         1869 g         3773 t
ORIGIN      
        1 atggggcaaa tgtggataaa gctgaaatgg caaaaacaca atacaaaacg gcactgtctt
       61 tgtttccaat gcacgaatca ggatggaaac cccaggtagt aacctgttcc gcattctaca
      121 aaacaaatat tgatgctatt tggacgatgt tgatgcaata tattcaattt actcaacaat
      181 caggatattt tgaaacaaaa aggcatcagc aagccagtta ctggatgtat gaaagtatca
      241 atcaagaatt aagaagtagt ttttatgccc actcagaagt gaatgcatta ctaaaagaaa
      301 tggaggtaaa agttacaaat ggagaaatta gctcctttat tgctgccaaa cgtttacttg
      361 atgtatatga caaaatccgg aaatagacaa atacaacttt ctttgtgaaa tcatttggta
      421 attatatttt catccttgtt atgttggtgg cttgttctca atataaaagt aaatctatac
      481 ttcaaggtac aatcactgat agtcggtata aaaaagtata tttattgcaa caaataacgc
      541 ctacatcatg ggaaatgttg gactctactt ctattgttga taatcatttt atgtttgctg
      601 atttaccttc tgaatacaat caactatttg ttcagttgga taatacaatc ccttttgtag
      661 tgtttgttga taagggaact attgagttag ctataccagc ccaaaatgta catgaaacaa
      721 aggtaaaagg atcttcttta catgatgaat acatgatgtt acaagatagt attgattttt
      781 tattgcatca acgtttgttg ttttataaag atgctattgt tgcacaaaat gatggaagac
      841 agacagatgc aacaatactt caacaaaaat atatccatag caaaaataat gtatttcatt
      901 ttcttcagaa acaaaaagga gcaaatccta cttctgctcc tttgttatat ataattaaaa
      961 catataaacc gtattttacc gacacggaat atcgaacatt atctgcttct ttagagtaat
     1021 ttattcttta tcggcaactc cttcaacggt aatttgacgt ttgaatgttt cacctagtga
     1081 catcagtgtt tctgtgctag ctattccttt tatactttgt agtttgtcat gaataatgct
     1141 caaaaaatca ctattattgc gtgcataaat tttaataaac atcgcatatt ttcctgttgt
     1201 ataatgacat tccactactt cgggtatttt tagtagttcg tcgactactt ctttaaagaa
     1261 attagcatct ttcaaaaata ttcccatgta tgcacatgta ttgtaaccaa ttttgactgg
     1321 gtcaattata aattcggaac cttttattac acctactgaa attaattttt gcacacgttg
     1381 gtgaatagcc gcaccagata cattgcattc acgagcaact tctaaaaaag gcgtgcgtgc
     1441 atttttggaa attaattgaa ggattttttc gtctaaactg tctaattgat actgtgccat
     1501 gcgtattttg cttttaaatt atatgcaaaa atactatttt tatttcatat tacgtatttt
     1561 gttatcattt tttagtatta aaattatata taccaatggg ttactttttt gttgtctatc
     1621 ttacaatttg ttactctact tgagttgttc ggcttcaaac ttcctaatct ttttttaatt
     1681 aattgcattt atttttggta aaaaatgtat ctttgtgcct tgaaatattg ttgtaaagta
     1741 ttatttatat ggcaactaag cgtttaaagg aaattaaacc tattgaggag actgccgata
     1801 tttggacact gttaagctca gaccaagccc attatttgca aacgaactta caggttataa
     1861 gtgccaaaaa aaatgaagta atttattcag acggtgaaat tccttctcat ttattttgtc
     1921 ttgttagtgg caaggttaaa gtgtataaac aaggcatagg aggacgtaat cagattgtcc
     1981 gcgttataaa acccaatgaa ttatttgggt atagggcaat gtttgctctc gaggcttatg
     2041 taacctgtgc ttctgctatt gagccttgtg ttatttatgc tattccagag gatgttattg
     2101 tttctattat aaaaaataat gctcaacttg cgtttgcttt tatccaattt ttagctgttg
     2161 atttgggaat ttctgattca agaacagtaa cccttactca aaaacatatt cgtggtcgat
     2221 tagcagaatc attgctgttt ttacgtgata attatggcgt tgaatctgat ggtatcactc
     2281 tatctattta tctttctagg gaggatttag caagcttgtc aaacatgacc acttccaatg
     2341 ctattcgaac attatcgtta ttttcaaacg aaggaatgat tgttgtagag ggtagaaaaa
     2401 ttaaaatagt agatgtagag tctcttgtaa aaatcagtaa atttggttga tttattcttt
     2461 tcgtaacgta aaaacaaatt ctaatccccc tgtttcgtgt ggttttgctg aaattctacc
     2521 ttgatgaaat gcaatagcat ttttaacaat agctaaaccc aatcctgttc ctcccatttt
     2581 acgagaacgt cctttgtcaa tacgataaaa acggtcaaaa atacgggtta aatggttttc
     2641 agataccccc actcctgtgt cagcatacga aaaatagtag aaatccttgt ctgttcggta
     2701 acaattaatg ataatactaa tgtcgttgcc agcataatgt aggctattat caataaaatt
     2761 tctaaagatg gaatatagta acgaatagtt gcccattatt gtcatattgt gctttagaaa
     2821 cacttctact cgaatgtttt tttgttctaa ctcgagcgaa acatctttta gtactccttc
     2881 aacaatatca tttaatacaa tcttctcttt ttcaaatgtg taagaacctt cgtcgagttt
     2941 gttgagtaac gaaatgtctc gtaacaaata gcttaaacga acagcttgag catggcaacg
     3001 ttgaataaaa aaatcttttt tttcttgttc catgtcggga ttttctatca atgtttccat
     3061 aaaaccctga atactactaa caggcgtttt cagttcgtga gaaatatttt gtgtgagttg
     3121 cttttttatt aagttgtttt gaacttgttt ggagatgtca ttaattgaaa tctcaaaggt
     3181 tttatcttgg aaaataatac atcgaacgag caaggtataa tcgtttttgt ctatttctac
     3241 aattttgtct gttacctcat tgggattatt ttgattgtat tggttttcat ttaagaaact
     3301 agtgataggg gaaaattcgg ctaatgaaaa aatactatct gatgtgctga tttttttgtt
     3361 tgaaatcaaa ttaatgtatt gtatgaaatt ttcgtttgcc aaaatttctt ttctatcaaa
     3421 agtgtaaatc gctaagcctt cttgcgatat ttgaagatgt ttaaaaagtt tttctttctc
     3481 taacgataag gcatctttgg tggctgtaag gtttttatat gtgtttacga taaaactaga
     3541 tatctcttta aattctttgt ttaaaaaaag taaatcgtca tcaaaatctt ctttgttttc
     3601 catttttaaa attacctttt tgaggttttt tacggtatgc aatagcttat tgcttacaaa
     3661 ataaaacgct aagcttgata aacttagtac aaaaaacata aagaatgaag ccgtaaaatt
     3721 acgaatcttc caaacgtcac ttataatata ttcgtatgga attgaggcta aaatcgtgtg
     3781 tgtatcatat ttttttccat aaaagaagta ggaggatttt gtttggtcgt aacgtacaac
     3841 taatgtatgt tctggtgtgt ttttttctgt aatcattccg aatgcacgtt cagatacatt
     3901 gtaaataacc gaatcattca ttggtgtgga ataaatacat tcgccactcg tattataaat
     3961 agagaagtac acatcttttt tttgcaattc atctaccagt gcgttgggaa tttcagtttt
     4021 tctagcagtc tttgtattta aatcaattat ctcggtataa aattgtaatg tttctgtata
     4081 acgtgccttt accatgtgtt tttcgtattg cacattgata ataaaaataa tagcggcaaa
     4141 aaacacaaaa ataccaacga atgataataa tagtttttta aacaaaatca tggttgtgga
     4201 ggcttgttag tttgatgttt caaaacaata tccatatcct agccgagtaa caacattttt
     4261 atcataaggt tctatttttt ttcgcagtcg agtaatgttt acatcaatag ttcggtctgt
     4321 aacatcagcg ttattccata tttgttgtaa aatgtcctct cgagaaaaaa cattgttggg
     4381 ttggctcatt aataagctta aaatttcaaa ttcttttttt gttaaattta tttcttgttt
     4441 atcaatgagt gttgttttag tagttaagtt cacaaacaat tgctcaaaac gcaaaatttt
     4501 atctttcgac atgggggttc cgtctttttg ctccgtagtt cgttttaata caactcgcac
     4561 tctagcaact acttctttca ctgagtaagg ttttgtaata taatcatcag cccctattgt
     4621 aaaaccagtt aaggtgtctg tttctgtatc tttagctgta atgaaaatga ttggaatatg
     4681 cgaggttgtt tcgtcctttt tgagcataga accgagttta aagcccgaca tagctcccat
     4741 cattacatcc agtaaaaata gattgtattg tgttaggtct ttttttaatg cgtcttcggc
     4801 agaataagca atgtctacga tatatccgtc tttctctaaa ttgtattgta gtatgtcgca
     4861 caaatcttct tcgtcgtcta ctaccaaaat tctaaatgta ctcatagtgc ataggttttt
     4921 ttacaaagat atttatttta ttgctatttt gcacgttaag cgataatatt ttcttgctga
     4981 atatgtttta gtatagattc catagaggta tatgagtcgt aggatacata taatcagatg
     5041 attttgtggt gttagtattc attttggcaa catgtagaat gttatataat tgtaatatgg
     5101 catagtaatt tgcttttttt atagtataat gtacgcaaat atagtgtatt tttgtacaat
     5161 taaatatatt gtgcgatgaa tagaaaatac cgattgggac ttgtattgag tggtggtgga
     5221 gcaaagggtt ttgctcattt gggcgtgtta aaagccatcg aagaaaaagg actgaagccc
     5281 gatattattg ctggtgtaag ttcaggtgct ttaatggggc tattgtatgc tgatggtaaa
     5341 agcttagatg aaattatgaa atttttcgag aaagcctcct tgtttgactc catatcgcca
     5401 accctgccta aattgggttt aatgaatcct tcaaaattta aacgtcagtt acgcagtttt
     5461 ttaacggcaa agtcatttga agagcttgct attccttttg tagtgaatgc aacgaatctt
     5521 actgacggca aaatgacggt gtttcgtaca ggtgaatctt tagtagaagc agttattggc
     5581 tcttcgagtg ttccgttatt atttactccc cagattatcg aagggaaaca atatgtggat
     5641 ggaggtgttt tttgtaatat gcctgtttcc ccaattcgcg atgagtgtga ctatattatt
     5701 ggagttcatg ttaatccaat ttgtaccgaa accaaagccg aaagtattat cgaaatagca
     5761 gagcgagtac ttcattatgc catacaatca agcacgatta acgaaaaaaa attatgcgat
     5821 gttgtaattg atgtaaagga agtaaaaaag tatggtatgt ttaatttaga taaaaaacaa
     5881 gaaatttttg attatggcta tacgaaagct aaggaagtat tagaaaattt ctatatagaa
     5941 taagactata cacaatatac atgttcatat aatgctgttt tgacagtcaa aaaatcttct
     6001 tccgaaagtg catggggata gctcatgata tgcatgggga cagcatctgt atgttgatgc
     6061 agtgctggtt gaaaataggg gtattggttg acgtgaaacc cattacgtac ataaaactca
     6121 attcttcgtt ttgaaatgtc ttgtgtagca tgttctactt cgagaataag gggtgttgtg
     6181 ctctcttttt ttattaactc aagtacttta ttccctatac ctaatccttg ttttgcaggc
     6241 gtaattgcta aataataaat aaatacaaaa aaagtatatt ctactatact cagcatgcca
     6301 caagtttcat ttttatctag gattgcatat agcttaaagg cacagtcatc agtaaattta
     6361 tgcgacaata aagaagaaaa atctactttt tcttctggcg gaaaggcttc ttcgtaaata
     6421 gattgaatgc tgggcaagag aggatgattg ttgtgagaga taaattcaag tgaaatcata
     6481 aactatataa tttaccgaaa atggttactc tattatagta agtcttatat acaaataggc
     6541 tgtctttgac agcctatttg agattgtaaa attattcagt atctacagca tcttgaatgc
     6601 tacattcaat aagtataggt aaatggtcag aaggataagt agtgtgagct aatttttcgt
     6661 ctgctgcaat gtaagaatta acgtgtactt tgtcgtttat aaatatgtaa tcaattcgtt
     6721 ttgaaacctt gtcattgcta ctgtataaac ccgtataggt atgttctttg ttaatatatc
     6781 ccccttgacg caatgtagga gtagtttcgt aagcatcatg ccattctcct gataatatag
     6841 tatatgcagg ctctgtgggt tcgcaattaa aatctcctgt gaaaattact ggatagcctt
     6901 cggttaaagc taatatttgt tctctggcaa tagtcgcttg ttgcttacgt gtatcgatgc
     6961 ctttttgtgt agtggggtgg tcgaaatgtg ttgaaaataa gaaaaatact ttttgagtct
     7021 gtttatcttc gagttgtacc catactacca tacgattgaa tgtagaggtg ggaagttttg
     7081 aaatcgagtc tggtgtgttt gataaaaaga aacgtccttt nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn ccagaatttt ccacccgtat ctcctgttgt gtaataacga
     7261 atattatacg acccaataat atatttttct tgttcatcta ttgcttcaaa agatgctgta
     7321 attgtggtgt cttgtgttaa taccaaataa ggtgttgtat tgatattgct aaaaacatgg
     7381 ctattattgc caaaagcaat cacagaaagt tccgtgtttt caggcacttg gtctccacta
     7441 tttactacat tccctttatt cagtaccgta atagttcctt gctcgttttg ttcaatcgta
     7501 accgtataca taaccattgg ctcaaatact gctgtaattg tggtatcttg ggttagctca
     7561 aaaggtgaac ttacgtttac actttccaag gtgtaaaatt catcaggggt agctgtaacg
     7621 gtaaggctgg tgccttttat tatttcagtt ccgttttcaa ttacggtatt tccttgtttt
     7681 acggtaatgg ttccgccttc gggttgttca atagtaacgg tgtacttgat tatttctggt
     7741 tctttttcct tagcactaca tgctgttaaa agaagaactg ctgtaagcag taagtaattc
     7801 catgttttca tacgtatagg ttattaattg attaaaaagt tatttaattg ttggtgtatt
     7861 cgcttagctc taaccatcgc attgttttat cgtcaattag ttctatcact tcagcaatac
     7921 gttcagatgc ttttataagc tcttgttgag ggatgcttcc tcctgataac attgtttcaa
     7981 ggtctttttt ttcagtttcc aaattttcta tgtctgattc tagcaatgaa tattcacgtt
     8041 gttcggcaaa actcaacttt cttgtacggt taacagagcg tttgggttct tcaactttat
     8101 ttttttgttt aatttctgct tgtttggcat caatagcatt ttgttgctct ttccattctc
     8161 ggtaatcgga gtaattacca ggaaaatctt tgataacagc attgccttga aataccaata
     8221 agtgatcaac taccttgtca gtaaaatacc tatcgtgcga aacgactatc acgcagccct
     8281 taaaattagc taaatattct tccaatacat ttagggtaac aatatccaag tcgttggtag
     8341 gttcgtcgag caccaaaaaa ttggggtttt tcatcaaaac agtacatagg taaaggcggc
     8401 gtttttctcc tccactaagt ttgtacacat aattatgttg tgtttcgggt gtaaacaaaa
     8461 aatgttgcaa aaattgggat gcacttagtt ttttgccgtt tcctatattt atttcttcgg
     8521 caatgtcgcg tactacatca atcactttca tttgttcatc aaactttaac ccatcttgac
     8581 tgtagtagcc aaaagaaacg gtttcgccaa tatcaaatga gccactgtcg ggtttaataa
     8641 ttcctaaaag caattttaaa aaagtagatt ttcctgtacc atttttacca attatgccca
     8701 ttttctcgta tcgggaaaaa ttatagtaaa aatcttttat caacacaaaa tcgtcaaatt
     8761 gtttgctaat atacttggct tcaaaaattt ttgaacccaa ataagtggcg ttcatttgca
     8821 gttttaccga ttcatcttga cgtttttgtg ccactttttt ttctaaatca taaaaactgt
     8881 caatcctgct ttttgccttc gttgcccttg cttgaggttg tcgtcgcatc caatctaatt
     8941 cttttctgaa taaattgttt gcacgtgcta actcagcatt ttgggcatca atgcggtctt
     9001 gtcttttttc taaataatag ctgtaattgc ctttgtatgc gtataactgt ttttggtcta
     9061 tttctataat gtcagtgcac acacggtcta aaaaatacct atcgtgagtt accatcaata
     9121 aggttaatcc cgaacgaata aggtactctt ctaaccattc ggtcatttct aaatccaaat
     9181 ggttggtagg ctcatctaaa ataagcaagt cgggctctgt tatcaaagca ttagccaaag
     9241 ctacccgttt gagttgaccg ccagataagt gtttaatctt tttgtcaaaa tcagtaatct
     9301 tgagttgtgc taaaatttgc ttaatcttaa tatcataatc ccaagcttta cgaatttcca
     9361 tttgttctac caattcttga aattctgggc tatctaaatt ttcgtttttg ctgatatatg
     9421 cttcgtattg tgcaattagg cgaattgttt cgttgttcga agaaaaaaac gtatccaata
     9481 tagtctgttc tggattgtaa gatgggtctt gctctaaaaa agaaagcgtt atatcccggc
     9541 gaaaggttat tttcccttcg tctaaggatt ctttccccat gattatattg agtagggtgg
     9601 atttgccagt tccgtttttt gctattaggg ctacacgttg cccctcgcta atatccatat
     9661 ttagcccttc gaaaagcagt aaatcgccaa agcgtttact cacattttca agttgtatgt
     9721 agttaattac tgccattaag tctttttata aataactagc aaatgtacat ataaaactct
     9781 aaagtagcaa tactccaaaa acgatacatg caaatcttgt ctaaaaaata gccttattgg
     9841 ttaattttta ttacttttgt ggctcaaaat ttgagtgtaa aaatggcaaa agaattaaaa
     9901 gatttgacca aacgtagcga aaattattcg cagtggtaca acgatttagt agttaaagct
     9961 gatttagctg aacagtcagc agtaaggggt tgtatggtaa ttaagcccta tggttatgct
    10021 atttgggaaa aaatgcaaca agaattagac cgtatgttca aagaaacagg tcatgtgaat
    10081 gcatattttc cattattaat tcccaaatct tttttaagta aagaagctga gcacgtagaa
    10141 ggttttgcta aagaatgtgc cgtagtaact cattatagat taaaaaatgc tgaagatgga
    10201 tcgggtgtag tggttgaccc agctgccaag ttagaagagg aattaattat tcgtccaacc
    10261 tccgaaacta ttatttggaa tacttaccgc aattggatac aatcgtaccg cgatttaccc
    10321 attttatgta atcaatgggc aaacgtattt cgttgggaaa tgcgtacacg tatgtttttg
    10381 cgtacagcag aatttttgtg gcaagaaggg catactgctc atgctactaa agaagaggct
    10441 gaagaagaag cattaaaaat gctgcacgta tatggcgatt ttgcagagaa ttttatggca
    10501 gttcctgttg taaaaggcat aaaaacagcc aacgaacgtt ttgctggagc cctcgaaact
    10561 tataccatcg aagctatgat gcaagatgga aaagccttac aatcgggcac ttcgcacttt
    10621 ttaggacaaa attttgcgaa agcttttgat gtgcagttta ttacca
//
LOCUS       DAOSRP010000063        10559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6137_length_10559_cov_4.887947, whole genome shotgun sequence.
ACCESSION   DAOSRP010000063 DAOSRP010000000
VERSION     DAOSRP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_10559_cov_4.887947"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..963
                     /gene="ftsY"
                     /locus_tag="PLJ82_05145"
     CDS             19..963
                     /gene="ftsY"
                     /locus_tag="PLJ82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOR40925.1"
                     /translation="MGLFSFFSKDKKETLDKGLAKTKESVFSKITRAVVGKSKVDDEV
                     LDNLEEALLASDVGVETTLRIIERIEKRVADDKYMNTDELNGILKQEIIDLLAENNTD
                     FLQDTNEEGKPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKSVYLGAADTFRAAAVEQL
                     CIWGERVNVPVIKQKMGADPASVAFDTISSAKANNADVVIIDTAGRLHNKVNLMHELS
                     KIKNVMQKVIPTAPHEVLLVLDGSTGQNAFEQAKQFTKATQITALAITKLDGTAKGGV
                     VIGISDQFKIPVKYIGLGEKMEDLQLFDKTAFVQSLLG"
     gene            970..2271
                     /gene="rimO"
                     /locus_tag="PLJ82_05150"
     CDS             970..2271
                     /gene="rimO"
                     /locus_tag="PLJ82_05150"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOR40926.1"
                     /translation="MKKNHIDLITLGCSKNLVDTEKLMYQLKVAGYTVAHDAENPTGE
                     IVVINTCGFIGDAKEESIDMILAFAEKKKKRKINKLFVMGCLSQRYLQELAIEIPEVD
                     KFYGKFNYTDLLKELGKEYRFDCSLQRTITTPMHYSYIKISEGCNRTCSYCAIPLITG
                     KHQSRKIEDIEEEVRWLVKQGVKEFQLIAQDLSYYGLDNYKKHKLAELTNRLADIDGV
                     KWLRLHYLYPVDFPMDVLNVMRERDNVCNYLDIALQHISDNMLKKMRRGISSQQTYDL
                     IRQIREQVPGIHIRTTMLLGHPGETDEDVEALKECVRTMRFERLGAFAYSDEEATYSN
                     KHYVDNIPYEIKQQRVDEIMKIQQQISLEHNQAKIGKNFKVIVDSLQGDYYVARSEYD
                     SPEVDMEILLKAEKNHLQIGEFYTATIIGAEEFDLYAEIIK"
     gene            2309..2602
                     /locus_tag="PLJ82_05155"
     CDS             2309..2602
                     /locus_tag="PLJ82_05155"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR40927.1"
                     /translation="MNNKELISELSKRLRVSQNDISNLLASTVSAFSTAFQQGNDVSL
                     QGFGIFQIRKKNERIIVNPSTNVRQIVPPRLTVIFKMSNTYKEKIKNIIPHGK"
     gene            2592..>3028
                     /locus_tag="PLJ82_05160"
     CDS             2592..>3028
                     /locus_tag="PLJ82_05160"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR40928.1"
                     /translation="MENKKQSIQELVDIIATQTNFTKKNSEEFLKEFVHVLQEYLEKD
                     GFVKVKGLGTFKLVKNEERKSVNVTTGEAIIIPAHNKVSFIPDNAIKEAVNKPYAHLE
                     TITVDDDTKNSTIDAQPDNTVREEKSIQDEAKNNSIPNNTVIQ"
     assembly_gap    3029..3038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3039..3601
                     /locus_tag="PLJ82_05165"
     CDS             <3039..3601
                     /locus_tag="PLJ82_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40929.1"
                     /translation="NNSKKTKKNKKKNPVAIVILLLLFHVAMAVSFYFYKTEIVSFFQ
                     RAVTIEENNLPQATIIDNQLSPVSIVADTVVVVETDSMPDTTNIQQPDEIIATYTPEL
                     AQNAPIKEYVTVNEGSRLTWIAYKAYGHKAFWVYIYDANRNQLKTPNDVKIGMELAIP
                     ELDASLVNPNDENCVIKALELAEKYK"
     gene            3742..4737
                     /locus_tag="PLJ82_05170"
     CDS             3742..4737
                     /locus_tag="PLJ82_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR40930.1"
                     /translation="MSEIVDIRELNARIEQKSAFVEVLTIGMNKTIVGQKHLIESLLI
                     GLLSDGHILLEGVPGLAKTLAIKTLAQLIDATYSRIQFTPDLLPADVVGTMIYSQKKE
                     QFEVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTLAETTHKLPSPFLVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVVSYPKKEEERLIIRQNIGPEQKALVPVLTIKDIL
                     DARSIVKEIYIDEKIEKYILDIVFATRNPEEYGLAKLKDMIAFGVSPRASINLALAAR
                     AYAFIKHRGYVVPEDIRAVCYDVLRHRIGLSYEAEATNLTPEDIISEIINAVEVP"
     gene            4756..5625
                     /locus_tag="PLJ82_05175"
     CDS             4756..5625
                     /locus_tag="PLJ82_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOR40931.1"
                     /translation="METSEVIKRVRQVEIKTKGLSQNIFAGQYHTAFKGRGMAFSEVR
                     EYQYGDDIRNIDWNVTARFNHPYIKIFEEERELTVMLLIDVSGSREFGSVDKTKKNII
                     TEVAATLAFSAIQNNDKIGVLFFSDRIEKFIPPQKGKKHILHIIRELIDLHPKSRKTD
                     ISMVLKYFSNAIKKKCTAFILSDFIDTNDFEQAMLLANKKHDLVALQIYDKRETELPN
                     VGLLKIKDAETGEIVLVDSSNKAIRDLYQSVWNKKQYKLNQVFNRCRVDNVSIDTTED
                     YVKSLLKLFRLRS"
     gene            5629..6576
                     /locus_tag="PLJ82_05180"
     CDS             5629..6576
                     /locus_tag="PLJ82_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40932.1"
                     /translation="MYLNKFSWLIIFFFLSVPIAYCQTVTIDAELDSSSIIIGDQAIL
                     HIQITKPVAESIVIPQFTDTLVKGIDIISITKIDTTLVDDKQNIQFSIYLTSFEPGDY
                     TIPAIKFVGENDTYQTPLFPLLVKPFSINPDDEKLNDIKPILQMPFSWKTFFKWTAIV
                     LLVLALVAGIVYILVKYVFKKKIPLVKSKEEIIPPHIIAIQELDKIKAEKLWQQGKVK
                     EYYSAITDVIRVYLERRFDIPALEMTTHELVKLISTNPETLPIKDTLSELLHLSDIVK
                     FAKYIPLNSDNDLSLIHSYFIIDKTKIEPITEPTSEKDM"
     gene            6578..7561
                     /locus_tag="PLJ82_05185"
     CDS             6578..7561
                     /locus_tag="PLJ82_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOR40933.1"
                     /translation="MVFQHPYILFLLFLLIPYIAWYIWKRRTLTPEIQFPTTGVLAQA
                     PKTPRMLLRHVPFVLRCLSVASIIFVIAQPQKRDSWKNETTEGIDMMLVMDISGSMLA
                     EDLRPNRLEAAKSVAVEFISGRTNDNIGLVAFSGKAFTQCPLTINHKELINLLLSLQT
                     GMIEDGTAIGNGLATAISRIKESPAVSKAIILLTDGANNRGEIAPITASEIAASYGIR
                     LYTIGVSTYGVAPYPFQTPTGIVYQNIDEDIDDSTLEQMATSTGGRYFRATDNNSLRA
                     IYQEIDKMEKTRIHVKEYSQREELYFIFALLACCFLLVEILLKNTLLKSIP"
     gene            7575..8591
                     /locus_tag="PLJ82_05190"
     CDS             7575..8591
                     /locus_tag="PLJ82_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOR40934.1"
                     /translation="MFKFAHPEYLYLLSLIPLLWLFFIYSMIIKNKRLSKIGSKHIIK
                     QLMPDASHWRPRIKFYLSLLAFTVALLMLARPQLASKSETVKRKGVELMIVLDVSNSM
                     LANDVSPNRMEASKRLVSRLIDELQDDKVGLIVFAGDAFVQMPITTDVLSAKMFLSSI
                     NTGIVPTQGTAIGAAIQLAMQSFGPPTAAERAIIIITDGENHEDDPIILAQEASEMGI
                     KTHVLGVGTPQGSPIPIADTADFWRDSEGNVVVSQLNEQMCKEIALAGSGTYVQVDNT
                     NTALRVLKSQLDTMSKAEMETKVYSAYDDYFRLLAIITLVILLVDVFITERKNHRLSK
                     VKLF"
     gene            8691..9311
                     /locus_tag="PLJ82_05195"
     CDS             8691..9311
                     /locus_tag="PLJ82_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR40935.1"
                     /translation="MKVSLVLFLLVIFVPVFSQDVTNDVRKGNKLYNDKQYGDAEVMY
                     RKGQQKDKFSVESTYNLGNALYKQQNYEAAASEYKKATVLAKEDKTVSAKVYHNLGNA
                     LFYQNKYADSVEAYKMALKNNPKDDETRKNLVIAQKKLQEQQNSSSENKEQNDKKEEQ
                     PPKKEQISQENSDQILKAFIQEEKLIQQQLQQQKVNDTKRVLDKNW"
     gene            9318..10319
                     /locus_tag="PLJ82_05200"
     CDS             9318..10319
                     /locus_tag="PLJ82_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOR40936.1"
                     /translation="MRTSELIQTIIKTKYNTYISGSCVRLSREITSISSCTKYYAVIL
                     LLLFYGSSIANANNDVQFRASALENVVVGQQFRVTYTVNQEAKDFRGPDLSDFSVVMG
                     PSSSRSSSYQIINGNATSSFSLTYAYVLMANATGEFTIQPAHITVNGQRYASNSLRIK
                     VVAGNAGNANSAPSSSSGVVTQPNSNQSISDNQIFIKQQISKKKVYEQEALSVIYKLY
                     TRYDVRDVSNAKFPDFKGFFSQEIELPKDRQFTVENVNGVNYNTIVLKQYLLYPQRSG
                     NITIEGAVVDVIMRLSNNVRSRGIFDFFDTFQEVKKTLKTSSETISVKSLPTTISKS"
BASE COUNT         3821 a         1534 c         1934 g         3260 t
ORIGIN      
        1 tataactatc aaaataatat gggcttattt agtttttttt caaaagataa aaaagaaacc
       61 ttagataaag gcttagcaaa aacaaaagaa agtgttttct caaaaattac acgtgctgtt
      121 gttggtaaat caaaggttga cgacgaggta ttagataatt tagaggaagc tttgcttgca
      181 tctgatgtgg gagtagaaac tacattacgt ataattgagc gtattgaaaa aagggtggct
      241 gacgataaat atatgaatac tgatgaatta aatggtattt tgaaacaaga aattattgat
      301 ttattggcag aaaataatac tgatttttta caagatacga atgaagaagg taaaccatat
      361 gtgataatgg tggtgggagt aaatggtgta ggtaaaacca ccacaatagg caaacttgct
      421 tatcaattta agcaagcagg taagtcagtt tacttaggtg ctgctgatac ttttagagca
      481 gctgctgttg agcaattatg catatgggga gaacgggtaa atgtgcctgt tataaagcaa
      541 aaaatgggag cagaccccgc ctctgttgct tttgatacta tatcgtcagc taaggcaaat
      601 aatgctgatg ttgtgataat tgatactgct ggtcgtttgc ataacaaagt gaatttgatg
      661 cacgaattat ctaaaattaa aaatgtgatg caaaaagtga ttccaacagc acctcatgag
      721 gtattgttag tgcttgatgg ttctactgga cagaatgctt ttgagcaagc aaaacaattt
      781 acaaaagcta cacagataac tgctttggct atcacaaaat tagacggaac agccaaaggc
      841 ggagttgtta taggaatttc tgatcaattt aagatacctg ttaaatatat tggattgggt
      901 gaaaaaatgg aagacttgca attgtttgac aaaacggctt ttgtacaatc tttattagga
      961 taatttgcga tgaaaaaaaa tcatattgat cttattacct taggttgttc aaaaaattta
     1021 gttgatacag aaaaattaat gtatcaacta aaagtggcag gttatacggt agctcatgat
     1081 gctgaaaatc caactggaga gattgttgta attaatactt gtggatttat tggagacgcc
     1141 aaagaagaaa gtattgatat gattctggct tttgcggaaa agaaaaaaaa acggaaaata
     1201 aacaaattat tcgtaatggg ttgtctttca caacgttatt tacaagaatt ggctatagag
     1261 attccagaag tagataaatt ttacggaaaa tttaattata cagatttact caaagaattg
     1321 ggcaaagagt atcggttcga ttgtagcttg caacgtacca tcactacgcc catgcattat
     1381 tcatatatca agatatcaga aggatgtaat cgcacgtgct cttattgtgc gatacctttg
     1441 ataactggca aacaccaaag tcgaaaaata gaagatatcg aagaggaggt acggtggtta
     1501 gtaaagcaag gcgtaaaaga atttcaatta atagcacaag atttatctta ttatggtctt
     1561 gataattaca aaaaacacaa attagctgaa ttgacaaatc ggttagctga tatagatggt
     1621 gtaaaatggt tacgtttaca ttatttatat cccgttgatt ttccgatgga tgtgctgaac
     1681 gtaatgaggg agcgagataa tgtgtgcaat tatctagata ttgcactaca gcatatctca
     1741 gataatatgt tgaaaaaaat gagaagagga atatcttctc aacaaaccta cgatttgatt
     1801 cgccaaattc gagaacaagt gccaggtatt catattcgta caaccatgtt attgggtcat
     1861 cctggagaaa cggacgaaga tgtagaagct ctcaaggagt gtgttagaac tatgcgtttt
     1921 gagcggttag gagcatttgc ttattcagat gaagaagcta cttattctaa caaacattac
     1981 gtagataaca ttccttatga aataaaacaa caacgtgttg acgaaataat gaaaatccaa
     2041 caacagatat ctttggagca taatcaagct aaaattggta aaaatttcaa ggtaatagtg
     2101 gatagtttgc aaggagacta ctatgtggca cgttctgaat atgattctcc tgaggtagat
     2161 atggaaattt tacttaaagc agagaaaaac caccttcaaa taggagaatt ttatactgca
     2221 acaatcattg gggcagaaga atttgatttg tatgctgaaa taataaaata aatctgtata
     2281 tttgtcgttg tcttaaaact atattactgt gaataataaa gaattaatca gcgaattgtc
     2341 taaacgttta cgtgtgtcgc aaaatgacat aagtaatttg ttggcatcta ccgttagtgc
     2401 tttttctact gcatttcagc aaggaaatga tgtttcatta caagggtttg gtatatttca
     2461 aataagaaaa aagaacgaac gaattattgt caatccgtct actaatgttc gacagattgt
     2521 gccaccaaga ttaacagtga tttttaaaat gagtaacaca tacaaagaaa aaattaaaaa
     2581 cataatacct catggaaaat aaaaaacaat ccatacagga attagtcgat atcattgcca
     2641 ctcaaacaaa tttcacaaaa aagaattcag aagaattttt aaaagaattt gttcatgtat
     2701 tgcaagaata tcttgaaaaa gatggttttg taaaagtgaa aggtttgggt acttttaaat
     2761 tagtaaaaaa cgaagagcga aaaagtgtaa atgtaacaac gggagaagca attatcatcc
     2821 ctgcacataa caaagtttcc tttatcccag ataatgctat aaaagaagcc gtaaataagc
     2881 cttatgcaca tcttgaaaca attactgttg atgatgatac aaaaaatagt actattgatg
     2941 ctcaacctga taataccgtt agagaagaaa agtccataca ggatgaggca aaaaataata
     3001 gtattcccaa taacaccgta attcaacann nnnnnnnnca aataatagca aaaaaacaaa
     3061 gaaaaataaa aagaaaaatc ctgttgctat cgttatttta ttactccttt ttcatgtagc
     3121 catggcagtt tcattttatt tttacaaaac tgaaatcgtg tccttttttc aacgggcggt
     3181 aactattgaa gaaaataacc ttcctcaagc tactattata gacaatcaac tttctccagt
     3241 tagcatagta gctgatacgg ttgtagtagt tgaaacagat agtatgccag ataccacaaa
     3301 catacaacaa cctgatgaaa taatagcaac ttatacacca gagcttgctc aaaatgctcc
     3361 aatcaaggag tatgttacag taaatgaagg aagtcggtta acatggatag cttataaagc
     3421 ctatgggcat aaagcttttt gggtttatat ttatgatgcc aatcgtaatc aattaaaaac
     3481 tcccaacgac gtaaaaattg ggatggaatt ggctatccca gaattagatg catcgttggt
     3541 taatcctaat gacgaaaatt gtgttattaa ggcacttgaa ttagctgaaa aatataagta
     3601 ataataagat atagaaagaa agtcacgcca tgaatgtgtg gcttttttgt ttaacaattt
     3661 atcaaggttt ttaacaaggt tttaaaccaa gaatatcaga aaatagtgta atttcgtatc
     3721 cgtattaaaa caacccaaaa tatgagtgaa atagttgata ttagagaatt aaatgcacgt
     3781 attgaacaga aaagtgcttt tgtggaggtg ttaacaattg gaatgaacaa aacaattgta
     3841 gggcagaaac atttgataga atcattattg ataggtttac tgtctgatgg acatatcttg
     3901 cttgaagggg ttcctggttt agccaaaacc ttagccatta aaacacttgc acaattaatt
     3961 gatgctacat acagtcgcat tcagtttaca ccagatttgt tacctgctga cgtagtgggt
     4021 actatgattt atagtcaaaa gaaagagcaa tttgaagtaa agaaaggacc aatttttgct
     4081 aattttattt tagcagacga aataaaccgt gctccagcaa aagtgcaaag tgcactactt
     4141 gaggctatgc aagaaagaca agttacgttg gctgaaacaa cacacaagct gccttctcct
     4201 tttttggtat tagctactca aaatcctatt gaacaagaag gaacataccc tttgccagaa
     4261 gctcaggtag atagatttat gttaaaagtc gtagtgtcgt atcctaaaaa agaagaagaa
     4321 agattaatta ttagacaaaa tattggacca gagcaaaaag cccttgtccc tgtgttaact
     4381 attaaggata ttcttgatgc ccgttctatt gtaaaggaga tatatattga cgagaaaatt
     4441 gaaaaatata ttttagatat tgtttttgca acgcgtaatc cagaagagta tggtcttgca
     4501 aaattgaaag acatgattgc atttggcgtt tcgccacgtg cgtctattaa tttagcatta
     4561 gcagctaggg catatgcatt tatcaaacat cgtggatatg tagtgccaga agatattcga
     4621 gctgtgtgtt atgatgtatt acgacacaga ataggattaa gttatgaagc agaagccaca
     4681 aatttaactc ctgaagatat tattagcgaa ataataaatg cagttgaggt accttaatct
     4741 atcaacatcg aattgatgga aacaagtgaa gtaataaaac gtgtacgaca ggtagagata
     4801 aaaaccaaag gcttatctca aaatattttt gcagggcagt atcatacagc tttcaagggt
     4861 aggggtatgg ctttttcaga agtaagagaa tatcagtatg gcgatgatat acgtaatatt
     4921 gattggaacg taacagcccg atttaatcat ccgtatataa aaatttttga agaagagaga
     4981 gaattaaccg taatgttgct tattgatgtg tcgggtagcc gtgaatttgg gtctgtagac
     5041 aaaaccaaaa aaaatattat taccgaagta gctgctacac ttgctttttc tgctattcag
     5101 aataatgata aaattggggt attatttttt tcagatagaa ttgaaaaatt tatcccgcca
     5161 caaaaaggaa agaaacatat tttacatatt attcgcgaat taattgattt acatcctaag
     5221 agccgtaaaa ctgatatttc gatggtgttg aaatatttct ctaatgcgat taagaaaaaa
     5281 tgtaccgctt ttattttgtc cgattttatt gatacaaatg attttgaaca agccatgtta
     5341 ttggcaaaca aaaaacatga tttggtagca ttgcagatat atgataaaag ggaaacagaa
     5401 ttacccaatg tgggcttgct taaaataaaa gatgccgaaa ctggagaaat tgtactagtt
     5461 gattcgtcaa ataaagccat aagagaccta tatcaatccg tttggaataa aaaacaatat
     5521 aagcttaatc aggtatttaa tagatgtaga gtagataatg tttccattga taccacagaa
     5581 gattatgtaa aatccttact taaattattc cgtttaagat cttaatgtat gtatttaaat
     5641 aagtttagtt ggttgattat atttttcttt ttgagtgtgc caatagcata ttgtcaaaca
     5701 gttacaattg atgctgagtt ggattcttct agtattataa ttggggatca agctattcta
     5761 catatccaga taactaagcc agtagcagaa tcaattgtca tccctcaatt tacggatact
     5821 ctggtaaaag gtatcgatat tattagtata actaaaatag atacaacact tgtagatgat
     5881 aagcaaaata ttcagttttc aatttattta acttcttttg agccaggaga ttatactata
     5941 cctgctatca aattcgtagg cgaaaacgat acgtaccaaa cgccattatt tcctttgctt
     6001 gtaaaacctt tcagcataaa tcctgatgat gaaaaattga atgatataaa gcctatttta
     6061 cagatgccat ttagttggaa aacatttttt aaatggacag ccattgtatt gcttgttctt
     6121 gctttagtag caggtatagt atacattctt gtaaaatacg ttttcaagaa aaaaattccg
     6181 ttagttaaat ctaaagaaga aattattcct cctcatatca ttgccataca agaattggat
     6241 aaaattaaag cagaaaaatt atggcaacaa ggcaaagtaa aagaatatta ttcagcaatt
     6301 acagatgtga taagggtata tctcgaaagg cgatttgata ttccagcatt ggagatgaca
     6361 acacatgaat tagtaaaatt aatttcaact aatcctgaaa cactacctat aaaagatacg
     6421 ttgagcgaat tattgcattt atctgatata gttaagtttg ctaaatatat acctcttaac
     6481 agtgataatg atttgagttt aatacacagt tattttataa ttgataaaac aaaaattgaa
     6541 ccaataacag aaccaacttc agaaaaagac atgtaatatg gtatttcaac atccttatat
     6601 attattttta ttatttttac ttatacctta cattgcttgg tatatatgga aacgtcgtac
     6661 attaactcct gaaatacagt ttcctaccac aggtgtttta gctcaagcac caaaaacgcc
     6721 acgaatgtta ttgcgtcatg ttcctttcgt tttacgttgc ttatctgttg ctagtattat
     6781 ttttgtaatc gctcaaccgc aaaaacgaga tagttggaaa aacgaaacta cagagggaat
     6841 agatatgatg cttgttatgg atatttccgg aagtatgcta gctgaagatt tacgacccaa
     6901 tcggttggaa gcagctaaga gtgtggcagt cgaatttatt tctggaagaa cgaatgataa
     6961 tataggtttg gtagcatttt ctggtaaagc ttttacacaa tgtcccctaa cgattaatca
     7021 caaagaattg attaatttat tattgtcttt acaaacaggt atgattgaag atggaactgc
     7081 tataggtaac ggacttgcaa ctgctataag tagaataaaa gagagtccag cggtatccaa
     7141 agctatcatt ttattaacag atggagcaaa taatagaggc gagattgccc caataacggc
     7201 atcagaaatt gcagcatcat acggaattag attatataca attggtgtaa gcacgtatgg
     7261 tgttgctcct tatccttttc aaactcctac aggtatagtg tatcaaaaca tagatgaaga
     7321 tattgatgac tctacattgg agcaaatggc aacatctaca ggtggacggt attttagagc
     7381 aacagataat aatagtttac gtgctattta ccaagaaatt gataaaatgg aaaaaactcg
     7441 gatacacgta aaagagtatt ctcaaagaga ggaattgtat tttatatttg ccttgttggc
     7501 ttgctgtttt ttgttagtag aaattttatt gaaaaataca ctattaaaat caataccata
     7561 actcacatac gtttatgttt aaatttgcac atccagaata cttatattta ctatcactca
     7621 tacctttgtt atggttgttt tttatttata gcatgataat taaaaacaaa cgtttatcaa
     7681 agatagggag caaacatatt atcaagcaat tgatgccaga tgcatctcat tggcgaccaa
     7741 gaataaaatt ttatttgtct ttattggcat ttacagttgc tttattaatg ttagcaaggc
     7801 ctcagttagc atctaaatcg gagacagtga aacgaaaagg agtagaatta atgattgtac
     7861 tagatgtctc taattcaatg ttggctaatg atgtgtctcc taatcgaatg gaggcgagta
     7921 aacgcttggt aagtaggctt attgatgagt tgcaagatga taaagtcgga ttaattgttt
     7981 ttgcgggcga tgcttttgtg caaatgccaa ttactactga tgtattatct gcaaagatgt
     8041 ttttatcttc aattaataca ggaatagttc ctacgcaagg cactgctata ggagctgcta
     8101 tacaattggc tatgcagtca tttggaccac caacggctgc tgaaagagca attattatta
     8161 taactgatgg agaaaatcac gaagatgatc ccatcatatt agctcaagaa gcctctgaaa
     8221 tgggtataaa aacacatgta ttaggagtgg gtactcctca aggttctccg attcctatag
     8281 cagacactgc agatttttgg cgagatagtg aaggaaacgt ggtagtaagt caattaaacg
     8341 aacaaatgtg caaagaaata gctcttgcag gtagcggtac atacgtgcaa gtggataata
     8401 caaatacggc gttaagagtt cttaaatcgc aacttgatac aatgtcgaaa gccgaaatgg
     8461 aaaccaaagt atatagtgcg tatgatgatt attttagact gctagcgatt atcacccttg
     8521 ttatattatt ggtggatgtg tttattacag aacgaaagaa tcatcggtta agtaaagtga
     8581 aattatttta gtctaaaaca taaaacttaa actcgatata tgctttataa aagcatagtt
     8641 aatatggtta taatatgctt aatataaaaa atagaaatat caataatagt atgaaagtat
     8701 ctttagtcct atttttatta gttatatttg tgcctgtttt ttcgcaagat gttactaatg
     8761 atgtgcgtaa aggcaacaaa ttgtataatg ataaacaata tggcgatgct gaggtaatgt
     8821 atcgcaaagg gcaacaaaag gataaatttt ctgttgaaag tacatataat cttggtaatg
     8881 cattatacaa acaacaaaat tatgaagcag ctgcaagtga gtataaaaaa gcaacagtgt
     8941 tagcaaaaga ggataaaaca gtatctgcaa aagtatatca taatttaggg aatgctctat
     9001 tttatcaaaa taaatatgcg gatagtgtag aagcatataa aatggcttta aaaaataatc
     9061 ctaaagatga tgagacacgt aagaatcttg tcattgctca aaaaaaatta caagaacaac
     9121 aaaattcgag ttctgaaaat aaagaacaaa acgataaaaa agaagaacag ccacctaaaa
     9181 aggaacaaat ctcacaagaa aattcagatc agatactaaa agcttttata caagaagaaa
     9241 aattgattca gcaacaattg caacaacaaa aagtcaacga cactaaacgt gtgttagata
     9301 aaaattggta acataagatg agaacttcgg aattaataca gaccattatc aagacgaaat
     9361 ataataccta tatttcgggt agttgtgtac gtttatccag agaaataaca tctattagta
     9421 gttgcacgaa atattatgcc gtaattttac tattattatt ttatggttca tccattgcta
     9481 atgcaaataa tgatgttcag tttcgtgctt cagccttaga aaatgttgta gtaggacaac
     9541 aattcagagt aacttataca gtaaatcaag aagcaaaaga ttttagaggt cctgatttaa
     9601 gtgatttttc tgttgtaatg ggtccatcta gttctcgtag ttcaagttat caaattatta
     9661 atggtaatgc aacctcatct ttttctctaa catatgccta cgtattaatg gcaaatgcaa
     9721 caggagaatt tacaattcag cctgctcata ttaccgttaa tggacaacga tatgcatcta
     9781 attcattacg cattaaagtt gtggcaggaa atgccggaaa cgcaaattct gctccgtctt
     9841 catcaagtgg agttgtaaca caaccgaatt ccaatcagtc tatttctgat aaccagattt
     9901 ttattaaaca acaaatttct aagaaaaaag tatatgagca agaagctttg tcagtaatct
     9961 ataaattata tacgagatat gacgtacgcg atgtatctaa tgccaaattt cctgatttca
    10021 aaggtttctt ttctcaagaa attgaattac ccaaagatag gcagtttaca gtggaaaatg
    10081 taaatggagt aaattataat acgattgtgc tcaagcagta tctgctgtat ccccaacgtt
    10141 cgggaaatat aactattgaa ggtgctgtag ttgatgtgat tatgcgtttg tctaacaatg
    10201 ttcgttcaag aggtattttt gacttctttg acactttcca agaagttaaa aagacactta
    10261 aaacttcctc agaaaccata tcggtaaagt ccttgcctac aacaattagt aaatcataat
    10321 tttttgtaag attttcattt tctgatttaa ccatagatat tcctgtatca ttaataactt
    10381 gtgaagcaat attttctttt ccgctactaa aataaattgt agttttggaa taattatttt
    10441 ttgcatcacc ggtatcagcg attacgtagc cagatttttc caatgatagt ttaaggttat
    10501 tggcattgtc tttaatattt gttccattaa gaattttaat tgttgtttct agtttggtt
//
LOCUS       DAOSRP010000064        10513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6172_length_10513_cov_5.525435, whole genome shotgun sequence.
ACCESSION   DAOSRP010000064 DAOSRP010000000
VERSION     DAOSRP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6172_length_10513_cov_5.525435"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /gene="rplN"
                     /locus_tag="PLJ82_05205"
     CDS             <1..358
                     /gene="rplN"
                     /locus_tag="PLJ82_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR40937.1"
                     /translation="QESRLNVADNSGAKEVLCIRVLGGTGKRYATLGDVIVVAVKSVI
                     PSSDVKKGAVSKAIIVRTKKEVRRADGSYIRFDDNACVLLNAAGEMRGSRIFGPVARE
                     LRTANMKIVSLAPEVL"
     gene            379..693
                     /gene="rplX"
                     /locus_tag="PLJ82_05210"
     CDS             379..693
                     /gene="rplX"
                     /locus_tag="PLJ82_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR40938.1"
                     /translation="MTKLHIKKGDTVYVNAGDDKGTVARVLEVLVKEQRAIVEGVNMV
                     SKHTKPNAKNPQGGIVKKEASIHISNLNPTVKGDKPTRVGRKQVDGKLVRYAKKTGEE
                     IK"
     gene            693..1238
                     /gene="rplE"
                     /locus_tag="PLJ82_05215"
     CDS             693..1238
                     /gene="rplE"
                     /locus_tag="PLJ82_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR40939.1"
                     /translation="MSNKASLKKEYTERIAPALKKEFGYKSAMQVPVLEKIVLNQGLG
                     IAVADKKIIEIAINEMTAIAGQKAIATYSKKDISNFKLRKKMPIGVRVTLRREKMYEF
                     LERLVRVALPRIRDFKGIDSKLDGRGNYTLGIQEQIIFPEINIDSINKILGMNITFVT
                     SAKTDEEAYALLKEFGLPFKK"
     gene            1259..1528
                     /gene="rpsN"
                     /locus_tag="PLJ82_05220"
     CDS             1259..1528
                     /gene="rpsN"
                     /locus_tag="PLJ82_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOR40940.1"
                     /translation="MAKESMKAREVKRAKMVAKYAEKRTQLKADGNYEALQSIPKNAS
                     PVRLHNRCKITGRPKGYMRIFGISRIQFREMASKGLIPGVRKASW"
     gene            1603..1998
                     /gene="rpsH"
                     /locus_tag="PLJ82_05225"
     CDS             1603..1998
                     /gene="rpsH"
                     /locus_tag="PLJ82_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR40941.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKKELTKILLDKGYI
                     LNYKFEETGHQGTIKIALKYDSVNKVNAIKKLIRVSTPGLRKYVGHTDLPRVLNGLGI
                     AIISTSKGVMTDKEAREMNVGGEVLCYVY"
     gene            2021..2575
                     /gene="rplF"
                     /locus_tag="PLJ82_05230"
     CDS             2021..2575
                     /gene="rplF"
                     /locus_tag="PLJ82_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR40942.1"
                     /translation="MSRIGKLPITLPKGVTVTFANNVVTVKGPKGELQQTVHPNIMVA
                     IDNGVVTVARPNDDKENRAMHGLYRSLLNNMVIGVSEGYKQTLELVGVGYRVSNNGQI
                     LEFSLGYTHGIFLKLPNEIKVETKSERNQNPIVILESCDKQLIGQVCAKIRSFRKPEP
                     YKGKGVKFAGEQIRRKAGKSAGAK"
     gene            2594..2950
                     /gene="rplR"
                     /locus_tag="PLJ82_05235"
     CDS             2594..2950
                     /gene="rplR"
                     /locus_tag="PLJ82_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR40943.1"
                     /translation="MTEKIARRLKIKASVRGKISGTATKPRMSVFKSNSQIYVQIIDD
                     TAIEGSGKTIVSASSLDIKEKMTKTEKSTKVGEVIAKKAKEANIETVVFDRNGFLYHG
                     RIKELAEAARKSGLKF"
     gene            2956..3474
                     /gene="rpsE"
                     /locus_tag="PLJ82_05240"
     CDS             2956..3474
                     /gene="rpsE"
                     /locus_tag="PLJ82_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOR40944.1"
                     /translation="MAGKNNIVKTTNDIELKDRLVAINRVTKVTKGGRTFTFSAIVVV
                     GNEDGIVGWGLGKANEVTTAIAKGVESAKKNLVKVPVLKGTIPHEQFAKFGGAEVFLK
                     PASTGTGVVAGGHMRAVLESVGVTDVLAKSKGSSNPHNLVKATIAALTELRDAYTVAQ
                     NRGISIEKVFKG"
     gene            3489..3665
                     /gene="rpmD"
                     /locus_tag="PLJ82_05245"
     CDS             3489..3665
                     /gene="rpmD"
                     /locus_tag="PLJ82_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOR40945.1"
                     /translation="MATIKVKQVKSRIKCPKVQKLTLDALGLKKMNRVVEHEATPQIL
                     GMINKVKHLVVVEE"
     gene            3702..4148
                     /gene="rplO"
                     /locus_tag="PLJ82_05250"
     CDS             3702..4148
                     /gene="rplO"
                     /locus_tag="PLJ82_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR40946.1"
                     /translation="MNLSNLKPAGGSVKTCKRVGRGSGSGLGGTSTRGHKGAKSRSGY
                     SKKVGFEGGQMPIQRRLPKFGFNNVNRIEYKAINLATLQQLAEEKKLEKIGIAELLEA
                     GFIARKHNVKILGNGVLTAKIEVTAHAFSKSAIEAIEKAGGSVIKM"
     gene            4153..5490
                     /gene="secY"
                     /locus_tag="PLJ82_05255"
     CDS             4153..5490
                     /gene="secY"
                     /locus_tag="PLJ82_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR40947.1"
                     /translation="MKRFIETIKNILKIEELKSRLLLTFLLVVIYRFGSYVALPGVDV
                     NALSALSQQTEGGLMMLLDMFSGGAFSNASVFALGIMPYISASIVVQLLGMAFPYFQK
                     LQREGESGRKKMNQITRYLTVGILLVQGPAYLGNLKMQMGNVFPEGWYFIVSSVIILA
                     AGSMFVLWLGERITEKGLGNGISFIILVGIIAKFPGAIIGEFSSRLNDASGGLVMFLL
                     EIVFLLVVIAASILLVQGTRKIPVQYAKRVVGNQQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMFVPASLSGLFATDGNVSAFAQAFMDHTSFWYNLVFALMIIAFTYFYTAVTINPT
                     QMAEDMKRNNGFIPGIKPGKKTAEYLDSVMSRITLPGSFFLAIVAILPAFAGLLGISF
                     SFAQFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKNGKIKGRTGSIAAY"
     gene            5519..6289
                     /gene="map"
                     /locus_tag="PLJ82_05260"
     CDS             5519..6289
                     /gene="map"
                     /locus_tag="PLJ82_05260"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR40948.1"
                     /translation="MIYLKTHDEIELMRESNLLVSRTLAEVAKNIRVGIKTLELDAIA
                     EAFIRKNGALPAFKGYSGFPNTLCVSVNEQVVHGIPSDYMLQEGDVVSVDCGVCMNGF
                     YGDSAYTFLVGEVDNNVKSLLKVTKEALYKGIEQAIHGKRIGDIGNSVQEYCEKNGYS
                     VVREMVGHGIGKHLHESPEVPNFGKRGQGIVLKEGMVIAIEPMINLGKKEIAFKRDGW
                     TTVTVDGLPSAHFEHTVAIKKGRADILSTFDYIEEVLN"
     gene            6301..6519
                     /gene="infA"
                     /locus_tag="PLJ82_05265"
     CDS             6301..6519
                     /gene="infA"
                     /locus_tag="PLJ82_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR40949.1"
                     /translation="MAKQVAIEQDGIIIEALSNAMFRVELENGHEITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRISFRYK"
     gene            6538..6654
                     /gene="ykgO"
                     /locus_tag="PLJ82_05270"
     CDS             6538..6654
                     /gene="ykgO"
                     /locus_tag="PLJ82_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOR40950.1"
                     /translation="MKVRASLKKRTPECKIVRRKGRLYVINKKNPKYKQRQG"
     gene            6680..7060
                     /gene="rpsM"
                     /locus_tag="PLJ82_05275"
     CDS             6680..7060
                     /gene="rpsM"
                     /locus_tag="PLJ82_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR40951.1"
                     /translation="MAIRIVGVDLPQNKRGEIGLTYIYGVGRSSANKILEEAGVDRDI
                     KVKDWTDEQASKIRDIISAKFKVEGDLRSEVQLNIKRLMDIGCYRGIRHRIGLPLRGQ
                     STKNNARTRKGKKKTVANKKKTTK"
     gene            7099..7488
                     /gene="rpsK"
                     /locus_tag="PLJ82_05280"
     CDS             7099..7488
                     /gene="rpsK"
                     /locus_tag="PLJ82_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR40952.1"
                     /translation="MAKKTATTKKRVVKVDANGQAHISSSFNNVIVTLTNSDGQVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKVAFDLGLRKVKAYVKGPGNGRESAIRTIHG
                     AGIEVTEIVDVTPLPHNGCRPPKKRRV"
     gene            7610..8215
                     /gene="rpsD"
                     /locus_tag="PLJ82_05285"
     CDS             7610..8215
                     /gene="rpsD"
                     /locus_tag="PLJ82_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR40953.1"
                     /translation="MARYIGPKSKIARKFGEPIFGPDKVLSRRNFPPGQHGNTRRRKT
                     SEYGIQLKEKQKAKYTYGVLEKQFANVFDKANRNKGITGEVLLQLLESRLDNVVYRLG
                     LSPTRSGARQLVGHKHIVVNGKVVNIPSYQVKAGDVVGVREKSKSLEVISNALSGFNH
                     GKYSWLEWEQSSMSGKFLHMPERVDIPENIKEQLIVELYSK"
     gene            8220..9212
                     /locus_tag="PLJ82_05290"
     CDS             8220..9212
                     /locus_tag="PLJ82_05290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR40954.1"
                     /translation="MPILAFQKPEKVIMLEADSFHGKFEFRPLEPGFGITIGNALRRI
                     LLSSLDGFAITSFKIDGVEHEFATIPGVIEDVTNIILALKQVRFKQLVEGVDSEKVII
                     NVEKTEVFKAGDIGKYLTGFEVLNPELVLCTMDAKSSFHIELTINKGRGYVPSEENKL
                     PSQELGVIPIDAIYTPIVNVKYVVENYRVEQKTDYEKLVFEIDTDGSIHPKDALKEAA
                     RILIHHFMLFSDEKITLDISDSDSVEEFDEEALHMRQLLKTKLTDLDLSVRALNCLKA
                     ADVETLAELVSFNRNDLLKFRNFGKKSLVELDAKLLSLNLSFGMDITKYKLDKE"
     gene            9219..9731
                     /gene="rplQ"
                     /locus_tag="PLJ82_05295"
     CDS             9219..9731
                     /gene="rplQ"
                     /locus_tag="PLJ82_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOR40955.1"
                     /translation="MRHNKKFNHLGRKTAHRDAMLSNMAVSLIKHKRISTTLAKAKEL
                     RKFVEPMITKSKEDTTHSRRNVFAQLQDKNAVTMLFAEVSQKVADRPGGYTRILKTGF
                     RLGDAAQMCIIELVDYNENMLKDKVAKKSSKTRRAGKKTKAVAEKVATVKAPKEEVVT
                     EQETPTETVE"
     gene            9834..>10513
                     /gene="pyrH"
                     /locus_tag="PLJ82_05300"
     CDS             9834..>10513
                     /gene="pyrH"
                     /locus_tag="PLJ82_05300"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOR40956.1"
                     /translation="MVYKRILLKLSGESLMANKPYGIDENRLQDYAFQIQEIVNLGVE
                     VAIVIGGGNIFRGMSGVAKGYDRVKGDQMGMLATVINSLALASALESINVVSHVLTAI
                     RMEPIGEYYSKQKAIDYLKKGEVVIVSAGTGNPFFTTDTASALRAIEMETDVMLKGTR
                     VDGIYTADPEKDVNAVKFDEISYDEIYNRNLKVMDLTATTMCKENKMPIYVFDMDTVG
                     NLKKVLLG"
BASE COUNT         3797 a         1356 c         2217 g         3143 t
ORIGIN      
        1 acaagaaagt agactgaatg tcgcagacaa tagcggtgca aaagaagttc tttgtatccg
       61 agtattagga ggaacaggta aaagatatgc tacacttgga gatgttattg ttgtagcggt
      121 aaaatctgta attccatcaa gtgatgtaaa aaaaggtgct gtttctaaag caatcattgt
      181 acgtaccaaa aaagaagtac gtagagccga cgggtcttat atccgttttg atgacaacgc
      241 atgtgtgttg ttaaatgctg ctggtgaaat gagaggtagt cgtatatttg gtcccgtagc
      301 acgtgaattg cgtacagcaa atatgaaaat tgtatcgctt gcaccagaag ttttataacc
      361 ctttgaacaa tatacaaaat gactaaatta cacataaaaa aaggagacac cgtttacgta
      421 aatgcaggtg atgacaaggg tacagtcgct cgtgtgcttg aggttttggt aaaagaacaa
      481 agagctatcg tagaaggtgt taatatggtg tctaagcaca ctaaaccaaa tgcaaaaaat
      541 ccacaaggtg gtattgttaa aaaagaggcg tctatccata tttcaaactt aaatcctact
      601 gtaaaaggag ataaacctac acgagtagga cgcaaacaag ttgatggtaa attggtacgt
      661 tacgctaaaa aaactgggga ggaaattaaa taatgagtaa caaagcaagt cttaaaaaag
      721 agtatacaga gcgtattgct cctgctttaa agaaagagtt tggatacaaa tctgcaatgc
      781 aggttccagt tcttgaaaaa atagttttga atcaaggttt aggcatagcc gtagctgata
      841 aaaaaattat tgaaattgca attaatgaaa tgactgctat tgctggtcaa aaagctatag
      901 ctacctattc aaaaaaggat atttctaatt ttaaattaag aaaaaaaatg cctattggtg
      961 ttagggtaac tttaagaaga gaaaaaatgt atgaatttct cgaaagactt gttcgtgtag
     1021 ctttacctcg tatacgtgat tttaaaggta ttgatagtaa acttgatgga aggggtaatt
     1081 atacgctagg tatccaagaa caaattattt ttcctgaaat aaatattgat tcaatcaata
     1141 aaatattggg aatgaatatt acctttgtaa catctgcaaa aacagatgag gaagcgtatg
     1201 cattactaaa agaatttgga ttacctttta aaaaataatt gagcaaaaat actagaatat
     1261 ggcaaaagaa tcaatgaaag ctcgcgaggt aaaaagagcg aagatggtag ctaaatatgc
     1321 tgaaaaaaga acacaattga aagctgatgg caattatgaa gcattacaaa gtataccaaa
     1381 aaatgcttct ccagtaagat tacacaatcg ttgcaaaatt actggaagac caaaaggata
     1441 tatgagaata tttggtattt ctcgtattca atttcgcgaa atggcatcaa aaggtttaat
     1501 accaggtgta cgtaaggcaa gttggtaatt aataaacgaa taactaattt caatttttaa
     1561 atattgtccg gacggtccgg attaatttaa ctataataaa atatgacaga tccaattgca
     1621 gattatttga cacggttaag aaatgcgatt aaagcaaatc acagagtagt agaaatcccc
     1681 gcgtcgaatt taaaaaaaga gcttaccaag atattgttgg ataaaggcta tatcttgaat
     1741 tataagttcg aagaaacagg tcatcaaggt actattaaaa ttgctttgaa atatgactca
     1801 gttaataagg taaacgcaat aaagaaactt atcagagttt caacgcctgg tttacgtaag
     1861 tatgttggac atactgattt gccaagagtt ttaaatggtt taggcattgc aattatttct
     1921 acttcaaagg gagttatgac agacaaggaa gctcgtgaaa tgaatgttgg tggagaagtt
     1981 ttatgttatg tgtattaatt taatttgagg aggattaaca atgtctagaa taggaaaatt
     2041 accgataaca ttacccaaag gggtaactgt tacttttgca aataatgttg taactgtaaa
     2101 aggtcctaaa ggggaacttc aacaaactgt tcatccaaat attatggttg ccatagataa
     2161 cggagtagta accgttgcta gacctaatga tgacaaagaa aatagagcta tgcatggatt
     2221 gtatcgttct ttgcttaata atatggtaat aggtgtctca gaaggatata aacaaacact
     2281 agaattagtt ggtgtgggat atcgtgtttc taataatggt caaatacttg aattttcatt
     2341 aggttatact catggaatct ttctaaagct tccaaatgaa ataaaagtgg aaactaaaag
     2401 tgagagaaac caaaacccta tcgttatatt agagtcatgt gacaaacaat tgataggtca
     2461 ggtatgtgca aaaatacgtt cattccgtaa acctgaacct tataaaggaa aaggtgttaa
     2521 gtttgcaggt gaacaaattc gcagaaaggc tggtaaatca gcaggtgcta agtaatttta
     2581 aaaggaaaca atcatgacag aaaaaatagc aagaagatta aaaatcaaag ctagtgtaag
     2641 aggaaaaatt tccggcactg ctacaaaacc tcgtatgtct gtttttaaaa gtaattcaca
     2701 aatttatgtg caaattattg atgatacagc aatcgaggga tctggaaaaa ccatagtttc
     2761 tgcttcttct cttgatatta aagagaaaat gacaaaaaca gaaaaatcga ctaaagtggg
     2821 tgaagttatt gcaaaaaaag ctaaggaagc gaatattgaa acagttgttt ttgatagaaa
     2881 tgggttttta tatcacggta gaattaaaga attagcagaa gctgctcgta aaagtgggct
     2941 taaattttaa ataatatggc aggaaaaaat aatatagtta aaacaacaaa cgacatcgaa
     3001 ttgaaagata gacttgtagc tattaatcgc gttacaaaag ttacaaaagg tggtcgtacg
     3061 tttacatttt cagctatcgt agttgtagga aatgaagatg gtatagttgg ttggggatta
     3121 ggtaaagcaa atgaagtaac tactgctatt gcaaaaggag tagaatctgc taaaaagaat
     3181 ttggtaaaag ttcctgtttt aaaggggaca atacctcatg aacaatttgc taaatttggt
     3241 ggagctgagg tatttcttaa accagcatca acaggtacag gggtagtagc aggtggtcat
     3301 atgcgtgctg tattagagag tgttggtgta acagacgttc ttgcaaaatc aaaaggctca
     3361 tcaaatcctc acaaccttgt gaaagctaca attgcagcat taactgagct tagagatgct
     3421 tatactgttg cacaaaatag aggtatttca atagaaaagg tttttaaagg ttaataatca
     3481 caaacactat ggcaactata aaagtaaagc aagtaaaaag tagaattaaa tgtccaaaag
     3541 ttcaaaaatt gacattggat gctttgggat taaaaaaaat gaatagagtg gttgagcatg
     3601 aagctacacc gcaaatatta ggaatgatta ataaagttaa gcacttggtg gttgtggagg
     3661 aataataatc ctttatcaac aacataaaaa actgtataat aatgaatttg agtaatttaa
     3721 aacctgcagg aggttctgtt aaaacctgta aaagagttgg acgtggttca ggttcaggct
     3781 tgggtggtac atctactaga ggtcataagg gagcaaagtc tcgttctgga tattctaaaa
     3841 aagtcgggtt tgaaggaggt cagatgccta ttcaacgtcg cttaccaaaa tttggcttta
     3901 acaatgtaaa tagaattgaa tataaagcta ttaatttagc aacgttacaa caattagctg
     3961 aagaaaagaa gttagaaaaa ataggtatag cagagttgtt agaagcgggc tttattgcac
     4021 gtaaacacaa tgttaagatt ttaggtaacg gtgttttaac agcaaaaatt gaggtaactg
     4081 ctcatgcttt ctcaaaatct gcaattgaag ctattgaaaa agctggtggc tctgtaatta
     4141 aaatgtaaga acatgaaaag gttcatagag acaattaaaa atattttaaa gattgaagaa
     4201 ttaaaatcta gattactact aacctttttg ttagtggtaa tctatcgttt tggttcatat
     4261 gtagctctac caggtgttga tgtgaatgct ttatctgcac taagtcaaca aaccgaaggt
     4321 ggtttgatga tgttgcttga tatgttttca ggtggagctt tttcaaatgc ctctgtattt
     4381 gcattgggta tcatgcctta tatttctgct tctatcgtag tgcagttact tggcatggct
     4441 ttcccttatt tccaaaaatt acaaagagag ggagagagtg gaagaaagaa aatgaaccaa
     4501 attacacgtt atttaaccgt tggtattctt ttagtacaag gtcctgctta cttaggaaat
     4561 cttaaaatgc aaatgggaaa tgtgtttcct gagggttggt atttcatagt ttcttctgtt
     4621 attattttag ctgcaggaag tatgttcgtt ctatggctag gagaacgaat aacagaaaaa
     4681 ggtttaggaa atggtatttc atttattatt ttagttggga ttattgcgaa gtttccagga
     4741 gctataatag gcgaattttc ttctcgatta aatgatgctt ctggtggttt agtaatgttt
     4801 ttattagaga tagtcttttt attggttgtt attgctgcaa gtattttact tgtacaggga
     4861 actagaaaga tacccgtaca gtatgcaaag cgtgtggttg gaaatcaaca atatgggggc
     4921 gttcgtcagt atattccgtt aaaagttaat gccgctggtg tgatgccaat catttttgca
     4981 caagcaatta tgtttgtgcc tgcatcattg tcaggactat ttgcaacaga tggaaatgtc
     5041 agtgcttttg cacaagcatt tatggatcat acaagttttt ggtataattt agtatttgct
     5101 cttatgatta ttgcatttac ttatttttat actgctgtaa cgattaatcc tactcaaatg
     5161 gcagaagaca tgaaacgtaa taatggattt attcctggta ttaaaccagg aaaaaaaaca
     5221 gcagaatatt tagattctgt tatgtctcgt attactttgc ctgggtcttt tttcttagca
     5281 attgttgcga ttctgcctgc ttttgctggc ttgttaggga ttagtttttc ttttgcacaa
     5341 ttttttggtg gaacgtcatt actcattttg gtaggcgttg tattagatac tttacaacag
     5401 atagagagcc atttgttgat gaggcattat gatggtttga tgaaaaacgg taaaattaaa
     5461 ggaagaacag gatcaatagc tgcttactag cactggtttg ttgaaacaac gttgtttgat
     5521 gatatattta aaaacacatg atgaaataga gctgatgcga gaaagcaatc tattagtgag
     5581 tagaacgctg gcagaagtag ctaaaaatat tcgagtaggg ataaaaacac ttgagttgga
     5641 tgctatagca gaagcattta tacggaaaaa tggagctttg cctgcattta aaggatatag
     5701 tggatttcct aatactttgt gtgtttctgt aaatgaacag gtagtacatg gtattccttc
     5761 tgattatatg ctacaagaag gagatgttgt ttctgtggat tgtggagttt gtatgaatgg
     5821 attttatgga gattcagcgt atacgttttt agttggtgaa gttgataata acgttaagag
     5881 tttgttaaaa gttacaaaag aagctttgta taaaggaatc gaacaagcga tacatgggaa
     5941 aagaattggt gatataggaa attcggttca agagtattgt gaaaagaatg gatattctgt
     6001 agtgcgtgaa atggttggtc atggtatagg taagcattta catgaatctc cagaagttcc
     6061 aaattttggg aaaagaggtc aaggcattgt tctaaaagaa gggatggtaa tcgcaattga
     6121 accaatgatt aatttaggaa aaaaagagat tgcatttaaa cgagatggtt ggactactgt
     6181 aactgtagat ggtttaccct cagcacattt tgagcatact gtagctatta aaaaagggag
     6241 agctgacata ctctcaacgt ttgattatat tgaagaagta ttaaattagt aatttatttc
     6301 atggcaaaac aagttgcaat agagcaagac ggaataataa ttgaagcatt gtcaaatgcg
     6361 atgtttcgtg tagaattaga aaatggacat gaaataacag cacatatttc tggtaaaatg
     6421 cgtatgcatt atattaagat tttacctggg gataaagtgc gtgtggaaat gtcaccgtat
     6481 gatttaacaa aaggaaggat ttcttttagg tacaaataaa tattaaatat aagaataatg
     6541 aaagtaagag catcgttaaa aaagcgtact cctgaatgta agattgtaag gagaaaagga
     6601 cgtctttatg tgattaataa aaaaaatcca aagtataaac aacgtcaagg ataattaaaa
     6661 acaaatataa aagtttatta tggcgataag aatagtcgga gtagatttgc ctcagaataa
     6721 gagaggtgaa ataggtttaa cctatatcta cggagtaggt agaagtagtg ctaataaaat
     6781 tttggaagag gctggtgttg acagagacat taaagtaaaa gactggacag acgaacaagc
     6841 ttctaaaatc agagacatta ttagtgcaaa gtttaaagtg gagggcgatt tgcgttctga
     6901 agtacagctt aacatcaaac gattgatgga cattggttgt tacaggggaa ttcgtcaccg
     6961 tattggttta ccattgagag gccaaagtac aaaaaacaat gcacgtacac gtaagggtaa
     7021 gaaaaaaaca gttgccaata agaagaaaac aactaaataa ttaatctgag taaataagca
     7081 taaatatact aacaagatat ggcaaaaaaa acagctacta ccaaaaaaag agtagtaaaa
     7141 gtagatgcaa atggccaagc acacatctca tcatcgttta ataatgttat tgtaacgttg
     7201 acaaattctg atggtcaagt aatatcatgg tcatctgcag gtaagatggg ttttagaggc
     7261 tctaaaaaaa ataccccata tgcagcacaa atggctgcac aagattgtgc gaaagttgca
     7321 tttgatttgg gattaagaaa ggtgaaagct tatgttaaag gacctggaaa tggtcgtgag
     7381 tcggctataa gaacaattca tggagcaggg atagaggtta cagaaattgt agatgtaacg
     7441 ccattacctc ataatggttg tcgtcctcct aaaaagagaa gagtataaaa ataatcttga
     7501 cagattcgtt ttctttttgt gatgaattac attatttaat cctctctgta atcgcggcaa
     7561 caaaatcaaa acgaaaagtc ttcgtttaaa ttcataaaaa taaaaacaaa tggctagata
     7621 cataggacca aaatcaaaaa tagcacgtaa gttcggagaa cctattttcg gaccagataa
     7681 agtattgtca agaagaaatt ttcctccagg acaacatggt aatacgagaa gaagaaaaac
     7741 ttctgaatat ggcattcagt taaaggaaaa acaaaaagct aaatacacct acggtgtgtt
     7801 ggaaaaacaa tttgcaaatg tttttgataa agcaaacaga aacaaaggta ttacaggtga
     7861 ggttttgtta cagttattag aatcaagatt agacaatgta gtgtatcgtt tggggttatc
     7921 accaacacgt tctggtgctc gtcagttggt tggacacaag catattgttg ttaatgggaa
     7981 agtcgtaaat ataccatctt atcaagtaaa agctggcgat gtagtgggtg ttcgcgaaaa
     8041 atctaaatcg ttggaagtta tatcaaatgc attgtcaggt tttaatcatg gaaaatatag
     8101 ttggttagaa tgggaacagt cttctatgtc tggtaagttt ttgcatatgc cagaaagagt
     8161 agatatacct gaaaatatta aagagcaact aattgtagaa ctttactcaa aataatatta
     8221 tgcctatatt agcgtttcaa aaacccgaaa aagtaattat gttagaagct gattcattcc
     8281 atggaaagtt tgaattcaga cctttagagc ccggatttgg tattacgatt ggtaatgctt
     8341 taagacggat tctattgtct tcattggatg gatttgctat tacatcattt aaaattgatg
     8401 gtgtagagca tgaatttgca acaattccag gagttatcga agatgttaca aacattattc
     8461 tggctcttaa acaagttcgt tttaaacagc tggttgaggg ggttgatagc gaaaaagtta
     8521 taattaatgt tgaaaagacc gaagtgttta aggctggtga tataggcaag tatctaactg
     8581 gttttgaagt gttaaatcca gaactagttt tatgcacgat ggatgcaaaa agtagttttc
     8641 acattgagct tactattaac aaaggaagag gatatgtacc atctgaagaa aataaattac
     8701 ccagccaaga acttggtgta attcctattg atgctattta tactcctatt gtaaatgtaa
     8761 agtatgtagt ggaaaattat cgtgttgaac aaaaaactga ttatgagaaa ctagtatttg
     8821 aaatagatac agatggttca atacatccaa aagatgctct taaagaagct gcaagaatct
     8881 taattcatca ttttatgcta ttttctgatg aaaaaatcac tttggatatt tctgatagtg
     8941 atagtgtaga agaatttgat gaagaggctc ttcatatgcg tcaattgtta aaaactaaat
     9001 taactgattt ggatttatct gtaagagctt tgaattgttt aaaagcagca gatgttgaaa
     9061 cattagctga attggtttcg ttcaatcgta atgatttatt gaagtttaga aattttggta
     9121 aaaaatcatt ggttgaactt gatgctaaat tgttgagttt aaatctttca tttggcatgg
     9181 atattacgaa gtataaatta gataaagagt aaaacgaaat gagacataat aagaaattta
     9241 atcatttagg tagaaaaacg gcacatagag atgctatgtt gtctaacatg gcagtttcgc
     9301 taatcaaaca taaacgcatt agtacgacat tggcaaaagc aaaggaattg cgtaaatttg
     9361 ttgaacccat gattactaaa tcaaaagaag atacaacgca ctcaagaagg aatgtgtttg
     9421 cccaattaca agataaaaat gctgtaacga tgttatttgc agaggtttct caaaaagtag
     9481 cagatagacc aggtggatat actcgtatac taaagacagg ttttcgatta ggagatgcag
     9541 ctcaaatgtg tattattgaa ttggttgatt acaacgaaaa tatgttgaaa gataaagttg
     9601 ccaagaaatc ttctaaaacg cgtagagcag gtaaaaagac aaaagctgtt gcagaaaaag
     9661 tagcaactgt aaaagcccca aaagaagagg ttgttacaga acaagaaacg ccaacagaaa
     9721 cggtagaata gacttctgaa ttaggataga ataaggaaag gaatgagtat tatgcttgtt
     9781 ccttttttta gtataaaaaa ataatgtatc tttgttcgta aaatttttag gcaatggtgt
     9841 ataaacgtat attattaaaa ttaagtggcg aatcgctcat ggcgaataag ccgtatggga
     9901 tagatgaaaa tcgattacaa gattatgctt ttcagattca agagattgtt aatctaggag
     9961 tggaagttgc cattgtaatt ggaggtggaa atatttttag aggaatgagt ggcgttgcga
    10021 aaggctatga tagagtaaaa ggagaccaaa tggggatgct ggctaccgtt attaacagtt
    10081 tagcgttagc ttctgcatta gaatcaataa atgttgtatc tcatgttctt actgctatta
    10141 gaatggagcc tattggggaa tattatagca aacaaaaggc aatagattat ttaaaaaaag
    10201 gagaggtggt aattgtttct gctggtacag gaaatccctt ttttactact gatactgcct
    10261 cggcattgcg tgcaatagaa atggagacag atgtaatgtt aaaaggaaca agggtagatg
    10321 gtatatatac tgcagatcct gagaaagacg taaatgctgt taaatttgat gaaatttcgt
    10381 atgatgagat ttataatcgc aaccttaaag ttatggattt aactgccact acgatgtgca
    10441 aggagaataa aatgccaatt tatgtgtttg atatggatac tgttggaaac ttaaaaaagg
    10501 tgcttttagg aga
//
LOCUS       DAOSRP010000065        10443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6213_length_10443_cov_5.570466, whole genome shotgun sequence.
ACCESSION   DAOSRP010000065 DAOSRP010000000
VERSION     DAOSRP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6213_length_10443_cov_5.570466"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /gene="tpx"
                     /locus_tag="PLJ82_05305"
     CDS             <1..427
                     /gene="tpx"
                     /locus_tag="PLJ82_05305"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOR40957.1"
                     /translation="NFTLVNTGLADVSLADYKGKNVVLSINPSLDTSVCAATARKFNT
                     TLSQLANTVVLYITKDLPFAHNRFCTTEGIENVVTLSDYRNPEFATTYGVLVQDGALK
                     GLMARAIVVINPQGVVTYTEMVPEITTEPNYDNVIAALK"
     gene            complement(463..1770)
                     /gene="murA"
                     /locus_tag="PLJ82_05310"
     CDS             complement(463..1770)
                     /gene="murA"
                     /locus_tag="PLJ82_05310"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOR40958.1"
                     /translation="MASFVIEGGHSLSGEITPQGAKNEALQVICATLLTKQKVTIHNI
                     PSILDVNNLIDLLKNIGVRVEKISKHTYSFQADAIDIDFFTSTAYYKQSSALRGSVML
                     VGPLVARFGYAIIPKPGGDKIGRRRLDTHFVGIQKLGAEFTYDPTHQLYKIEAKKLQG
                     TYMLLEEASVTGTANILMAAVLAQGKTTIYNAACEPYLQQLCKMLNAMGAKISGIGSN
                     LLTIEGVESLGGCEHTILPDMIEIGSFIGMAAMTNSSITIKNVSYENLGIIPDSFRKL
                     GINIEVSGDDIIVPKQSGFTIESYIDGSIMTLADAPWPGLTPDLLSVLLVVATKAKGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATIIGLGENFRLRKAVMSSPDIRAGI
                     ALLIAAMSAEGTSVIHNIEQIDRGYEDIELRINQLGGKIKREA"
     gene            complement(1775..2386)
                     /locus_tag="PLJ82_05315"
     CDS             complement(1775..2386)
                     /locus_tag="PLJ82_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOR40959.1"
                     /translation="MEYYTEKEKLILPEYGRNIQQMVNHALTIEDKAERTRCVHSIIA
                     IMGKLFPHLRDVNDFKHKLWDHLAIMSNFTLDIDYPYEVPKQEILNTKPERIPYPSIH
                     IKYRHYGKTVDTMVKKAIEYPEGDDKKRFIELIANYMKKSFLTWNKEGVDDEKIYEDL
                     KELSNGQLIYTSESLKLKESKEILAQNKVNKSKQNIPHKKIKK"
     gene            complement(2420..3109)
                     /gene="tsaB"
                     /locus_tag="PLJ82_05320"
     CDS             complement(2420..3109)
                     /gene="tsaB"
                     /locus_tag="PLJ82_05320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOR40960.1"
                     /translation="MPILLHLETSTTVCSVCLSENGKIVYEVVDDKGQNHSELLGVFV
                     QKALQEAKKQHLQLDAVAVSSGPGSYTGLRIGVSMAKGLCYGFSIPLIAINSLKILAQ
                     GVVANHSIEHDAILCPMIDARRMEVYTAFFDARLNILKPTWAEVLHADTFETLHDKKV
                     YIFGNGAEKSIGIVQHPNIQFLPHQTPLASSMIVLAEEAFQQQQFEDMAYFEPFYLKE
                     FMTSIPKNKLA"
     gene            3258..4133
                     /locus_tag="PLJ82_05325"
     CDS             3258..4133
                     /locus_tag="PLJ82_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOR40961.1"
                     /translation="MIQSMTGYGKAVSEYENKKIVAEIKSLNSKQLDLSTRVSSFYRE
                     KDIEIRNEISQRVERGKVEFSVYVENATSDVTSKINTTIFQQYVEQLSQLAENSGIEK
                     PTNWFEIILRFPEVMKNETAELTDEEWAVVKTTLLASIEQLQLYRKEEGKALYAVLLQ
                     KIDNIQQYLEQTVQYESERIDKIKQRLHENLKAIDDKIIVDENRLEQELIYYIEKLDV
                     NEEKERLKHHLAYFKSTMQEPTSSGKKLGFIAQEIGREINTLGSKSNQSDMQRLVVLM
                     KDELEQIKEQVLNVL"
     gene            4143..4721
                     /gene="gmk"
                     /locus_tag="PLJ82_05330"
     CDS             4143..4721
                     /gene="gmk"
                     /locus_tag="PLJ82_05330"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR40962.1"
                     /translation="METTVQGKLIVLSAPSGAGKTTIMRYLLQQDLSLSFSISATTRQ
                     PRGTEVHGKDYYFFSVEDFRQKIADGAFVEYEEVYPGCFYGTLKSEVERLTSQGKHVV
                     FDVDVVGGVNIKKMYGKRALTIFVQPPSIEALQQRLQTRATDTPEMIATRIAKAAYEM
                     TFAQQFDVVIVNEDLPKAQQEAEQVIRNFLAQ"
     gene            4718..5287
                     /gene="nadD"
                     /locus_tag="PLJ82_05335"
     CDS             4718..5287
                     /gene="nadD"
                     /locus_tag="PLJ82_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOR40963.1"
                     /translation="MNIGLYFGSFNPIHNGHLAIASYVLEHTDLHEVWFVVSPQNPFK
                     ANDVLLPEQQRLHMVQLAIQAIPMMKASDVEFALPKPSYTYDTLQYLTQHYPHHSFAL
                     MMGADNAERMHEWKNATQLLGHYPIYVYPRDNDTPIATQHPDFKYVQAPLYPISSTQI
                     RNQIANNTDVSAWLHPAVYAYIKEHNLYK"
     gene            5734..6747
                     /locus_tag="PLJ82_05340"
     CDS             5734..6747
                     /locus_tag="PLJ82_05340"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOR40964.1"
                     /translation="MKKAAKILCIIALVIGIIWAIVGFFGSWVGGAVISAGQEVFAED
                     SQGANSTLESSVNVMLKLIGSFVIVIIGGVLGIIGSDKKPSKVKPLIFGVLTLICGFI
                     LFPLANYVAAAIYLIAGLLLVLAGLTTKVQEENANKENKKLILSGIILGVLILAVGGF
                     CLLAGSNVKETSTGTTENSEAVEQMRQDSIVDEQSSEKSKDPIIGYDKVTWGTTIQEV
                     KQLYPDIVEKKEKNGVRRFSEKLYDNGMQYRYFYFFQDKLYQVAVDYDVNLKHSLVDK
                     LTSIYGKFESGLDYYERYYNKHLTIYVFVYDNPSDSYINVSYIDPTVQEQIDKMKEDA
                     IIL"
     gene            6750..7544
                     /locus_tag="PLJ82_05345"
     CDS             6750..7544
                     /locus_tag="PLJ82_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40965.1"
                     /translation="MTQNQVHTAVQAFVQNFISNSYLHRCEHSLHIALYNELFLQAPQ
                     QFVFCQKHQTNFVHKEFPGRKTTDAPILTGRQLVDRTSIDIVVLSKQQVNSIKDFLAG
                     NLDVDFAFELSLEYGIEHLCWDIFKFITGSNRSIDSKNYIIHLYHKERVQSYFDTEKI
                     NGNTMWNENGYIVENSLNNKVEWCYYLINLCIAYSQKTNNDDYDKIMAIIKHFVCKKY
                     KSITTDNYDNYLPDTEFIKNILQNMKFIITDANPKKEKRFASLSNI"
     gene            7685..7903
                     /locus_tag="PLJ82_05350"
     CDS             7685..7903
                     /locus_tag="PLJ82_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HOR40966.1"
                     /translation="MPKYIYQRKNWIDFTWNDKKISVLLAEVRHLQGRILGKMSSLGF
                     GYQEEATLENITLDILKSSEIEGEKLNK"
     gene            7943..8218
                     /locus_tag="PLJ82_05355"
     CDS             7943..8218
                     /locus_tag="PLJ82_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40967.1"
                     /translation="MAGLVNSDRSIDGIVEMMLDATQCYENSLIEERLFGWHGALFPT
                     GRSGLYAIEVAKYRTGEMQVVSGGFGREKIHYEAVLAKNLSVRCLRD"
     gene            8600..10045
                     /locus_tag="PLJ82_05360"
     CDS             8600..10045
                     /locus_tag="PLJ82_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004378797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOR40968.1"
                     /translation="MNKTQLIELVTELTKQPNESEWVEFKLNYHSAEEIGERISALSN
                     GACIHNQPFGYLVFGVEDSTHLIKGTTFNAKLHKKGDEDLEHWLATRLNPRIDFSVYE
                     FDYDELRHISIYIIPATKSQPVEFLHQAYIRVNTITRKLNDFPEKQAKIWKKDIIPFE
                     KEIAKDNLSASDITKYLSTETYFDLIKIPYPSTQQGVIDKFIDEGLVVKSKGYAITKL
                     GALLFAKNFKDFETIERKSVRVIVYKGKNKVETQREQIGIKGYALGFEGLVDWINSQL
                     PANEEIGKALRKETRMYPEIAIRELVANALIHQDLTEKGFPMIEIFTDRIEISNPGTP
                     VVTPERFIDAYLSRNEKLADLMRRLGFCEEKGSGLDKVIFYNELYQLPAINVIVAENR
                     TKVSMYSYKPLNDLDKKDKIRACYQHACLKYVSNEKMTNQSLRDRFKIEDQNAAIASR
                     IIRDSLEEGVIKEDDPESKSRKYASYIPIWA"
BASE COUNT         3498 a         1800 c         1966 g         3179 t
ORIGIN      
        1 taattttacc ttagtgaata caggcttagc agatgtttcg ttagcagatt acaaaggaaa
       61 gaatgtagtt ttgagtatca atcccagttt ggatacgtct gtatgtgcag ctactgcacg
      121 caaatttaac accaccttgt cgcaattagc gaatacagtg gtgttgtaca tcaccaaaga
      181 tttacctttt gcacacaatc gtttttgtac tacagaaggc attgagaatg tggttacttt
      241 atccgattat cgtaatcctg agtttgctac aacgtatggc gtgttagtac aagatggagc
      301 cttaaaaggt ttaatggcac gtgctatcgt agttattaat ccacaaggag tggttacgta
      361 tacagagatg gttccagaaa ttaccaccga acctaattac gacaatgtca ttgctgcttt
      421 aaaataacag tactcaattg tctgaaaata aaaagagaag ctttatgctt ctctttttat
      481 tttaccacct aactggttga tgcgtaattc tatatcctcg tatcccctgt ctatttgttc
      541 aatattgtgt attacgcttg ttccttcggc actcattgct gctattagca aggctatacc
      601 agcacgaata tcaggcgaac tcattaccgc cttgcgtaac ctgaaatttt ctcccaatcc
      661 aataattgta gctctatgcg ggtcgcataa gatgatttga gcacccatgt caatgagttt
      721 atctacaaaa aataggcgac tttcgaacat tttttgatgt atcaaaacac tccctttggc
      781 tttggtagct actaccaata atacactaag caagtcgggt gttaatccgg gccaaggagc
      841 atcggctaag gtcatgatag agccatcgat atacgattcg attgtaaatc ccgattgctt
      901 tggcacgata atatcatcac cgcttacttc gatgtttatg cctaatttgc gaaaactatc
      961 agggatgata cccaggtttt cgtatgatac atttttaatt gtgatagaag agttggtcat
     1021 agccgccata ccgataaaac taccaatttc aatcatatcg ggtaatatgg tatgttcgca
     1081 accacccaat gattcaacgc cctcaattgt aagcaagtta gagccaatac cacttatttt
     1141 tgctcccatg gcatttagca tcttacataa ttgttgcaaa tacggttcgc atgccgcatt
     1201 gtagatagtc gttttgcctt gagctaatac ggcagccatt agaatgttag cagtacctgt
     1261 taccgaagct tcttctaata acatgtatgt tccttgtagc tttttggctt caattttgta
     1321 gagttgatga gtagggtcgt acgtaaactc ggctcccaat ttttgtatgc cgacaaaatg
     1381 cgtatccaac cgcctacgac caattttatc gccacctggt ttgggtatta tggcataacc
     1441 aaaacgagcc actagtggac ctaccaacat tactgagcct ctaagtgcac tgctttgttt
     1501 gtaataggct gtacttgtaa aaaaatcaat atcaatcgcg tcagcctgaa aagagtaggt
     1561 atgcttactt attttttcaa cacgtacacc aatgtttttt agtaaatcta ttaagttgtt
     1621 tacatccaaa atactaggga tattatgaat ggtaactttt tgttttgtta gcaatgtggc
     1681 acatatcact tgtagtgctt cattcttagc tccttgcggc gttatttctc ccgacaaaga
     1741 atgtcctcct tctattacaa aagatgccat aaacttactt tttaattttt ttatgcggta
     1801 tgttttgttt gcttttattc actttgtttt gagccagtat ctctttggat tctttgagtt
     1861 tgagcgattc gctcgtatag attaattgcc cattagagag ttctttcaaa tcttcgtata
     1921 ttttctcgtc atctacccct tctttgttcc atgttaagaa actttttttc atgtagttgg
     1981 caatcagctc aataaaacgt tttttgtcat cgccttcagg atattcaatg gcttttttta
     2041 ccatagtatc tacggtttta ccgtaatggc ggtattttat atgtatgcta ggatacggaa
     2101 ttcgctctgg ttttgtattc agtatttctt gttttggcac ttcgtatggg tagtcaatat
     2161 ccaatgtaaa gttcgacata atagccaaat gatcccaaag tttgtgttta aaatcattta
     2221 catcacgaag atgaggaaac agtttaccca taatggcaat gatgctatgt acacagcgtg
     2281 tgcgttcggc tttgtcctca atcgttaatg cgtgattgac catttgttgg atattacgac
     2341 catattcggg caagattaat ttctctttct ctgtgtaata ctccattgca gtatcgtaag
     2401 ttgttgtcat ataattttac taagctaatt tgtttttagg aatagaagtc ataaattctt
     2461 ttaggtaaaa tggttcaaag taagccatat cttcaaattg ttgttgttga aaagcctcct
     2521 cagcaagcac aatcatactc gatgccaaag gcgtttgatg tggcaaaaac tgtatgtttg
     2581 gatgttgcac aatgcctatt gatttttcgg ctccgttgcc aaaaatatat acttttttat
     2641 cgtgcaaggt ttcgaatgtg tcggcatgta gcacttcagc ccatgttggt ttcagtatat
     2701 ttaatcgtgc atcaaaaaaa gccgtatata cctccattcg tctggcatca atcattgggc
     2761 ataaaattgc atcgtgttca atcgaatgat tggctactac cccttgtgcc agtattttta
     2821 agctattgat agcaataaga ggaatagaaa agccataaca caatccttta gccattgaca
     2881 ccccaatgcg taaccccgtg taggaacctg gtccgctact tacagcaacc gcgtcgagtt
     2941 gcagatgttg tttttttgct tcttgcagag ctttttgtac aaaaacaccc agtaactcac
     3001 tgtgattttg ccctttatca tcaactactt catatacaat ttttccgttt tcggataagc
     3061 agacagaaca aacagtagta gaggtttcaa gatgcaatag tatcggcata tcatgtcttt
     3121 tgtaacgtac aaaggtacaa ttttttagct gaattatgat gattgcaact aaaatttttg
     3181 atttattcgt aggatatggc tgtttttgta gataaaagca gtatttttgt gtgcaacaat
     3241 aaactaaaca gataggtatg attcaatcga tgaccggcta tggtaaagct gtaagtgagt
     3301 acgaaaacaa aaaaattgta gccgaaatta aatcgctcaa tagtaaacaa ttagaccttt
     3361 ctacacgcgt gtcttctttt tatcgcgaaa aagatattga gataagaaat gaaataagcc
     3421 aacgcgtaga aagaggtaaa gtagagtttt cggtgtatgt agaaaatgcc accagcgatg
     3481 ttacatcaaa aattaatacc actatttttc aacagtatgt agagcagtta tctcaattag
     3541 ccgaaaattc gggcatagaa aaaccgacca attggtttga gataatactc cgttttccag
     3601 aggtgatgaa aaatgaaacc gccgaactga ccgatgagga gtgggctgta gtaaaaacta
     3661 ccctgcttgc cagtatcgaa caattgcaac tgtatagaaa agaagaaggg aaggcattgt
     3721 acgcagtgtt gctacaaaaa attgacaaca tacaacagta tttggaacaa accgtgcaat
     3781 acgaatcaga acgtatagat aaaatcaaac aacgcttgca cgaaaaccta aaagccattg
     3841 atgataagat aattgtagat gaaaataggc tagagcaaga attgatttat tacatcgaga
     3901 aattagatgt aaacgaagaa aaagaacgct taaaacacca tttggcatat tttaaatcta
     3961 ccatgcaaga gccaacatcg tcaggtaaga aactaggctt tatagcacaa gaaataggca
     4021 gagaaatcaa taccttaggc tctaaatcca atcaaagcga tatgcaacgc ttggtagtgc
     4081 tgatgaaaga tgagctagag caaataaaag aacaagtatt gaatgtattg taacaacagc
     4141 ttatggaaac aactgtgcaa ggcaaattaa tcgtactttc agcaccatcg ggagcaggca
     4201 aaactaccat tatgcggtat ttattgcaac aagacttatc cttatctttt tctatctcag
     4261 ccactacacg ccagccacga ggcacagaag tacatggcaa ggactattat ttcttttcag
     4321 tagaagattt tagacaaaag atagcagatg gagcatttgt agagtacgaa gaagtgtatc
     4381 ccggctgttt ttatggcaca cttaagtcgg aagtggaacg cctaacctcg caaggcaaac
     4441 acgtagtatt tgatgtagat gtagtaggag gggtgaatat aaaaaaaatg tatggcaagc
     4501 gagcactcac tatttttgtg caaccgccat ctatcgaagc cttgcaacaa cgcttgcaaa
     4561 ctagggcaac cgataccccc gaaatgatag caacacgcat agccaaagct gcttacgaaa
     4621 tgacatttgc acaacaattt gatgtagtaa tcgtaaacga agatttgccc aaagcacaac
     4681 aagaagcaga gcaggtgata cgcaattttt tagcccaatg aacattggtt tgtattttgg
     4741 ttctttcaat cccatacaca atgggcatct tgccatagca agctatgtgc tggagcatac
     4801 cgacttgcac gaagtgtggt ttgtggtttc gccacaaaat ccctttaaag caaacgatgt
     4861 attgctaccc gaacagcaac gcctgcacat ggtgcaattg gcaatacaag ctataccgat
     4921 gatgaaagcc agcgatgtgg aatttgcctt gcccaaacca tcttacacct acgatacctt
     4981 acaatacctt acccagcact atccacacca cagctttgcc ttgatgatgg gagctgataa
     5041 cgccgaacgc atgcacgaat ggaaaaatgc cacacaactg ctcgggcatt accccatcta
     5101 tgtgtatccc agagataatg atacacccat agccacacaa caccctgatt ttaagtacgt
     5161 gcaagcaccc ttgtacccca tttcttctac acaaatacgt aaccaaattg ccaacaatac
     5221 agacgtatcc gcatggttgc acccagcagt gtatgcctat atcaaagagc ataatttgta
     5281 taagtaaaag tttataccgc catttgtaaa atggtttggt gctattgcta cggagagaac
     5341 gataggaaaa cctttttata cccaacattt ccataccaag tatttgtttg gtataattaa
     5401 atctttaact ttgctgtgag taaattaaca aattggatat acgccataac tattgctacc
     5461 ataaaaaata tattattcta actgtcaagt tattatgttt tttataatgc aaatatatac
     5521 gccatgctat ggcgtatata cagatgttat gcacaagtga gacaatgcaa caacggaagc
     5581 aactgaaaag aaataaaaga acagttctga aaagagcaga acaaaaaaac agcagtttga
     5641 caacccttga aacaatgtcg aaagctaaca ccgaaatcaa cggagtagcc gaaagccgtg
     5701 aaagtggagg caaaataaaa attaatattt tatatgaaaa aagcagcaaa aattttatgt
     5761 atcattgcac ttgttattgg tataatttgg gcaatagtag gtttctttgg ttcttgggtt
     5821 ggcggagcag ttatatctgc tggacaagaa gtatttgcag aagattcgca aggagcaaac
     5881 tcaacattag aaagttctgt taatgtaatg ttgaagctta ttggcagttt cgtaattgta
     5941 attattggtg gtgttttggg tattattggt tctgataaaa agccatcaaa agtaaaacca
     6001 cttatttttg gagttttaac attaatttgt ggatttattt tatttccatt ggctaattat
     6061 gttgcagctg caatttatct tattgcaggt ttattacttg ttttggctgg tttaacgaca
     6121 aaagtccaag aggaaaatgc aaacaaggaa aataagaaac tgattttgag cggaataata
     6181 ttgggcgtac ttatattagc cgttggtgga ttttgtttgc ttgcaggtag caatgttaaa
     6241 gaaacttcaa cgggaacaac tgaaaattca gaagcagtag aacaaatgcg tcaagattct
     6301 attgttgatg aacaatcttc ggaaaaatca aaagatccaa ttattggcta tgataaggtt
     6361 acttggggta cgactatcca agaggttaaa caactttatc ccgatattgt agaaaaaaaa
     6421 gaaaaaaatg gtgttagaag atttagtgaa aaactctatg ataatggtat gcagtacaga
     6481 tatttctact tttttcaaga caaactttac caagttgctg tagactatga cgtaaatttg
     6541 aaacattctt tagtagacaa acttacttca atttatggaa aatttgaaag cggtttggat
     6601 tactatgaaa gatattataa taagcattta actatatatg tctttgtata tgacaatccg
     6661 tcagacagtt atataaatgt ctcatacata gatccaacag ttcaagaaca aatagataaa
     6721 atgaaagagg acgcaataat attatagcaa tgacgcaaaa tcaagtacat actgccgtac
     6781 aagcctttgt gcaaaatttt atcagtaatt cgtatcttca cagatgcgaa cattcattgc
     6841 atattgcact atacaatgag ttatttctgc aagcacctca acaatttgtg ttttgtcaaa
     6901 agcaccaaac aaattttgtg cataaggaat ttccggggcg aaaaacgact gatgcgccta
     6961 ttcttacagg acgacaatta gttgatagaa catcaataga tattgttgta ttatccaaac
     7021 aacaagtaaa ttcaataaaa gactttttgg caggaaatct tgatgtggat tttgcttttg
     7081 aattatcgtt ggaatatggc attgagcact tatgttggga catcttcaaa ttcattacag
     7141 gttcaaacag gtctattgac agcaaaaact acattattca tttatatcat aaagaacgtg
     7201 ttcaatcata ttttgataca gagaaaatca atgggaatac aatgtggaat gaaaatggat
     7261 atattgtaga aaattcgctc aacaacaaag ttgagtggtg ctattattta ataaacctct
     7321 gcattgctta ttctcaaaaa accaataatg atgattacga taagataatg gcaatcatta
     7381 agcatttcgt ttgtaaaaaa tacaaatcta taactactga taattatgat aattatttgc
     7441 ctgatacaga atttattaaa aatatattac agaatatgaa gtttattatt acggatgcaa
     7501 atcctaaaaa agaaaagcga tttgcttcat tatcaaatat ataatttttt cgccgtttta
     7561 accaaaatgc ctaaaaacaa tgaaatgcga agaaaaagat aatttttctt cgcatttttt
     7621 gcgtagataa gtgttactat tctccgcaaa agttgtaatt ttgcattttc aaaattaatt
     7681 tgatatgcca aaatacattt atcaacgaaa aaattggatt gattttactt ggaacgacaa
     7741 gaaaataagt gtattattgg cagaggttcg tcatttacaa gggcgaattt tgggcaaaat
     7801 gagttctctt ggttttggtt atcaagaaga agcaacactc gaaaatatta cgctcgacat
     7861 tttaaaatcg tcagaaattg aaggtgaaaa acttaataaa tagcaagttc ggtcgtctat
     7921 tgctcggcgt ttaggacttg aagtggcagg acttgtaaat tctgacagga gtattgacgg
     7981 aattgtagag atgatgcttg atgcaacgca atgttacgaa aattcattga tagaagaacg
     8041 attgtttggc tggcacggag cattatttcc aacaggacgt agcggacttt atgctattga
     8101 ggttgcaaaa tacagaacag gagaaatgca ggttgtttcg ggaggttttg gacgtgaaaa
     8161 aatacattat gaggcggttt tggcaaaaaa tctctctgtc cgttgcttgc gagattaacc
     8221 ctccctgttc gccgtagtgt gaagtttgac aatctggctg ttcggcatag cgtctcgcta
     8281 tgtcgaagtt ataccgattg cacaaacaat atagcccaat ttcgacaaag tctcattgtt
     8341 cgctactcct cgttttcaca acgaggggtt tatggtttgt cgtttgtaac gacagtaata
     8401 cactaaaccc caaacatttt agtcaatgta cctcattata ttttgaaagt tcgttaaaac
     8461 ccattacatt tgagcaagaa ttaaactaaa cggaggtttt tagatggact gccgttgcta
     8521 acaagtgtag acgagtttct gacaaaatgt gagtatgctg taaataaatg aattatcttt
     8581 gacggcaaaa ttataatgaa tgaataaaac acaacttata gagcttgtaa ctgaattaac
     8641 caagcaaccg aatgaaagtg aatgggttga atttaaactg aattatcatt ccgctgaaga
     8701 aattggtgag cgtatttcag ccttgtcaaa tggagcatgt attcacaacc agccttttgg
     8761 ctatttagtc tttggcgttg aagacagtac tcatctaata aagggaacaa catttaatgc
     8821 aaaattacac aaaaaagggg atgaagattt agaacattgg ttggcgacac gtttgaaccc
     8881 cagaattgat ttttcagttt atgaatttga ctatgatgag ctacgacata tttcaattta
     8941 tataataccc gcaactaaaa gccagcctgt agagttttta catcaagcat atattcgggt
     9001 aaatacaatt acgagaaaat taaatgattt tcctgagaaa caagcgaaaa tttggaagaa
     9061 ggacattatt ccgtttgaaa aggaaattgc aaaggataat ctaagtgcat ctgacattac
     9121 aaaatattta agcaccgaaa cgtactttga tttaattaaa atcccatatc cttcaactca
     9181 acaaggggtt attgataaat ttattgatga aggattagtg gttaaaagta aagggtacgc
     9241 aattacaaaa ctaggtgcat tactttttgc aaaaaatttt aaagattttg aaacgattga
     9301 gcgaaaatct gttcgggtaa ttgtatataa aggaaagaat aaagtagaaa cgcaaagaga
     9361 gcaaattgga ataaagggtt atgctttagg atttgaagga ttagtggatt ggattaatag
     9421 tcaacttcca gcaaatgaag aaataggaaa ggctttgcga aaagaaacta gaatgtaccc
     9481 cgaaattgca ataagagaat tagtcgcaaa tgctcttatt caccaagacc taacagaaaa
     9541 aggatttcct atgattgaaa tctttactga cagaatagaa atttcaaatc caggcacgcc
     9601 agttgttaca ccggaacgat ttattgatgc gtatctttct agaaatgaaa aattagctga
     9661 tttaatgaga aggcttggtt tctgtgaaga aaaaggtagc ggtttagata aagtaatatt
     9721 ctataatgaa ctttaccaac taccagcgat taacgtaatt gtagctgaaa atagaactaa
     9781 agtgagcatg tatagttata aacctttaaa cgacttagat aaaaaagata aaataagagc
     9841 atgctatcaa catgcttgtt tgaaatatgt gtcaaatgaa aaaatgacaa atcaatcact
     9901 tcgtgacagg ttcaaaatag aagaccaaaa tgcagcaatt gcatcaagaa ttattagaga
     9961 ttccttggag gaaggagtta ttaaagaaga tgaccctgaa agtaaatcca gaaaatacgc
    10021 aagttatatc ccaatttggg cataaaatct tatttgatgg ttatttgata gaaaacatca
    10081 gttttagtat aatacattga atatcaatta cttgttattt gatagttatt tgattaggac
    10141 ataaaacacc agttggcaac atcatgtata aaacactggt ttttaagcgg ttattcaaac
    10201 attctgccac gcattaagct tggtgtaact ggactgtctt gtcctgaaat aggtctacac
    10261 aaaaagtgta aaattaaaca aaaaaaaaca aggtcttttg ggattaccga gaaagaaata
    10321 ttctttcata aaaatttcta ttccgctccg ctagagctaa caaacaaatc ggtatgtatt
    10381 ttctattatg atgtagcccc tatggggcaa gcgaaatagc attttcccct aaaaattggt
    10441 aaa
//
LOCUS       DAOSRP010000066        10383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6256_length_10383_cov_4.608249, whole genome shotgun sequence.
ACCESSION   DAOSRP010000066 DAOSRP010000000
VERSION     DAOSRP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_10383_cov_4.608249"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLJ82_05365"
     CDS             complement(<1..567)
                     /locus_tag="PLJ82_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HOR40969.1"
                     /translation="MKKIELVEVYIYNELVGKIALTDKGLCAFEYSTTFLQKGFSISP
                     FELPLKPGVFVAKLHPFSGNFGVFDDCLPDGWGMIILERYLQKIGVRVRMLSLLDLLS
                     IVGSSGRGALEFRPDQSIHTSQELQDFSYISNEAQKILRFSEYNAESIDDLYRRGGSP
                     GGARPKIFVMVDGKEWLVKFGAINDPINI"
     gene            complement(589..894)
                     /locus_tag="PLJ82_05370"
     CDS             complement(589..894)
                     /locus_tag="PLJ82_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR40970.1"
                     /translation="MILLNQKSVSEICVDIAQKVKQRRLELNLTQKGLALRADVNIET
                     YRKFERIGEISLVNLVKIAIALNAVDDFTSLFSQQKYQRIEDVLKTGSSSRKRGTIL"
     gene            complement(1175..2197)
                     /locus_tag="PLJ82_05375"
     CDS             complement(1175..2197)
                     /locus_tag="PLJ82_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOR40971.1"
                     /translation="MSNKIKFAVVGLGHIGKRHAEMIRRNASSELVAVCDILPKDKID
                     PEITETYYSDFDEMLAKQPDIDVVNVCNLNGHHAEYAIKALQAKKHVVLEKPIALSKS
                     DAEKVVFKSLEMSRSVFCVMQNRYSPPMAWLKELVENDTLGKIYFVKVDAFWNRDEKY
                     YKRGSWHGTNKLDGGTLFTQFSHYVDMLYWLFGDITNIRGKFANCSHGDLIEFEDTGC
                     VDFEFINGGMGSLNYSTSVWDKNLESSVTIIGEKGTVKIAGQYMDQVVHCHIKDYEMP
                     ELKQPELNNDFGGYKGAAQNHGFVIQNVIDTLKAKGAIGTNLLEGMKVVDIIERIYQE
                     RDRSFK"
     gene            complement(2194..2766)
                     /locus_tag="PLJ82_05380"
     CDS             complement(2194..2766)
                     /locus_tag="PLJ82_05380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOR40972.1"
                     /translation="MSYFKHESAYVDEGCQIGNGTKIWHFSHIMPNCTIGESCNIGQN
                     VVISPDVILGRNVKIQNNVSVYTGVICEDDVFLGPSMVFTNVINPRSHVNRKAEYAKT
                     LIKKGASIGANSTIVCGNEIGEFALIGAGAVITKNILPYALVVGNPARQIGWVSEYGH
                     RLNFDEKGIAICPETQQEYQLSNNTVKRIK"
     gene            complement(2768..3901)
                     /locus_tag="PLJ82_05385"
     CDS             complement(2768..3901)
                     /locus_tag="PLJ82_05385"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOR40973.1"
                     /translation="MKKIQMVDLKSQYEKIKEEVNAGIQEVIDTTTFIKGGKVNVFQK
                     NLETYLHVKHVIPVGNGTDALQIALMALGLKAGDEVITPTFTFIATAEVIALLGLTPV
                     VVDVEEDTFNISIDALKKAITPKTKAIIPVHLFGQCANMDAIMEVANKHKLFVVEDAC
                     QSIGSVYTFKDGRKVQSGCIGNIGCTSFFPSKNLGCYGDGGAIFTNDDTLAAAMRAIA
                     NHGMVVRYYHDMIGVNSRLDSIQAAVLDVKLKNLDTYIANRQAAAKAYNKAFANHPKI
                     TIPYEFPQSTHVYHQYTLKLKNVDRAALQKALEAKGIPAMVYYPVPLHMQKAYQDARY
                     KEGDFPVAEALSACVISLPMHTELDEEQLNYITSEFLTAINNQ"
     gene            complement(3937..5721)
                     /locus_tag="PLJ82_05390"
     CDS             complement(3937..5721)
                     /locus_tag="PLJ82_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOR40974.1"
                     /translation="MSIVIQINKKKNCTLCWRLIFFCFALFLSQTAYAQANAEHISYS
                     RIYDFLDELANDGIIELNSSIKPYSKNFIASKLQEAQKADSLLTKRQKGDLVFFMQNY
                     ALFYQNFPTSHLPLVQKNAWNVALVEPAVHYKDNLVSAKITPLLGMHIYSNRHGAITH
                     RWFGADMYMNIGKNIAVYGSLRDNSYSGEYLSSLPVNAARLSQPAYLNDLPGVEYKEA
                     NYGGDFSDSRGGITLHNEWGSIGLIKDNVSWGDNYNGSNILSGRAPSFPMITMKLKPT
                     SWFELNYIHGFLISNVLDSSYYYIEQHTIDGEKKQYRPMNKFVAANMLTFTPIPKLNI
                     SVGNAIVYAERSIQPAYLIPIAFYKSIDHLLTKGIKTENQNSQFFINVSSRNIKHLHL
                     YGSMFVDEIKFSRFLPSNPETNPISYKGGFHLSNFPISNLSFTTEFTYSTMVNYKHSI
                     ESLTWASNSYNLGHYLGDNSQELFVSLQYKPIQKLSVQLAYIQALHGNEYSYIRRSVK
                     EVISQPVLQDITWSNNRLISKASYELWNNAYAIASVEYNHAKGYNVTSTPIEAEIRKD
                     AQGYLDMYSPTFYQGQNITYTIGFSLGF"
     gene            complement(5718..7244)
                     /locus_tag="PLJ82_05395"
     CDS             complement(5718..7244)
                     /locus_tag="PLJ82_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOR40975.1"
                     /translation="MTKALKLYRPGIRFILIDVVILLANFLLITFWLKAVDTITAQSY
                     PLYISFSVLWMLFHYLFKHYKKNSIEKEYVAKSFKSIYATVIAATINFALFEYALLLP
                     VSIINYSIFVLGVLLLSLVHTTLHFAYLYALDIEEVAPIEIRPPASLVKEPYELDNDT
                     VHTIELSIVKETNARVLDYLGKVINLKSSATKLLSSGSIFNFEKLRTYRYDTIINLKR
                     LNDIRGINVLFSKINEKLPDNGIYIGCFENSGVKKRDILKKYPKGINWVIYSFYYLVK
                     RVIPKIFITRRLYYDITQGKNRVLTKTEVYGRLYYCGFEIIGEKKIGDQLFFVARRKQ
                     EPNPRKKRRYGPVIQLKRIGKNGRVFKFYKMRTMFPYSEFLQAYIYEHNKLQEGGKFS
                     HDIRISTLGKFFRRFWIDELPMILNFLKGDMKLVGVRPISQHYFSLYSKELQEKRIKF
                     KPGLLPPFYADMPKTLEEIESSEMRYLQMCEKKGVLITDIIYFWKIFVNIVFKKARSK
                     "
     gene            complement(7482..8795)
                     /locus_tag="PLJ82_05400"
     CDS             complement(7482..8795)
                     /locus_tag="PLJ82_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR40976.1"
                     /translation="MQKFYNREKEIEQLTKIEQLSKKIAQMTFVIGRRRIGKTALLKK
                     AFTETAMLYFFVEKKNEALLCEEFIQEIQYKLHIKVYGEFRTFKSIFGYLMELSKTTN
                     FTLIIDEFQDFHSVNSSIYSEIQNVWDANKDNSKINLILCGSIYSLMKRIFENAKEPL
                     FGRATARIHVKAFDIKTIKTILNDYTSENNNENLLAFYLFTGGVAKYVEMLVHAGAFT
                     LEDMVNEIFSENSLFLDEGKNVLIDEFGKEYGNYFSILSLIANSKTSRSEIESILHIS
                     IGGYLEKLENDYALITKVRPILAKPGTKNVKYKLNDNFLNFWFRFIYKYRGAVETESF
                     DYLKELFLRDYTTYSGQILERYFKEKLTNEMTLSQIGSYWESGNQNEIDIVALNEYEK
                     KAIIAEVKRNKDKIDLRVLEEKAQNLSKKLTGYTITYKGFSLDSM"
     gene            complement(8924..9733)
                     /locus_tag="PLJ82_05405"
     CDS             complement(8924..9733)
                     /locus_tag="PLJ82_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40977.1"
                     /translation="MKDFTLQAYKDLLIALQQSAYQFITFEQYCIAKQTNSSLPEKVV
                     ILRHDVDLKAANSLATAQLEAELGIVASYYFRVVPQSNQPEIITQIAALGHEIGYHYE
                     DMGIFNGNHVQAIAHFQQQLAYFRTYYPVKTICMHGNPTSKWDSKDLWKHADYKDYGI
                     IGEPYYDIDFSQFFYLTDTGRCWDGYNVSVRDKVPLHQDNWNSRGLKFHSTYDIIKGI
                     ENGLLTKEGINQIMITTHPQRWTNSNKEWILEFIIQNLKNTIKYILSKRNK"
     gene            complement(9768..9974)
                     /locus_tag="PLJ82_05410"
     CDS             complement(9768..9974)
                     /locus_tag="PLJ82_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40978.1"
                     /translation="MKVVIIAGGKGTRIASVAADISGYDGGKLKQIAHHGVHIPVNDM
                     QITEDLHMVLDHGIMSILSSHLGF"
     gene            complement(10050..>10383)
                     /locus_tag="PLJ82_05415"
     CDS             complement(10050..>10383)
                     /locus_tag="PLJ82_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40979.1"
                     /translation="ESATMVAEGAMLGVPGVYIDNTGRFYTRDIAQKYNLCFNYSESE
                     SDQLLAIEKGVDVLKHNASQEYKRKNRAQLLADKIDVTAFLMWVVLEYPKSIQQLRLN
                     PDIQQQFK"
BASE COUNT         3271 a         1845 c         1581 g         3686 t
ORIGIN      
        1 aatatttatg gggtcattta tagcaccaaa tttaacgagc cattctttac catctaccat
       61 tacaaatatt tttggacgtg caccacccgg cgaacctcct ctacgataca aatcatcaat
      121 agattctgca ttatattcag agaatctgag tattttctgg gcttcgtttg atatatatga
      181 gaaatcttgt aactcttgtg aagtatgaat actttggtcg ggacgaaatt ctaatgcccc
      241 cctacctgaa ctaccaacta tagatagaag gtcgagcaaa gacaacatac gcactcttac
      301 tccaattttt tgcaaatatc gctcaagaat aatcatcccc caaccatcgg gcaaacaatc
      361 atcaaaaacg ccaaaatttc cgctaaatgg gtgcaatttt gcaacaaata cacctggctt
      421 taatggcaat tcaaatggtg agatagaaaa tcctttttgt aaaaaagtag tactatactc
      481 aaatgcacac agtcctttat cagtaagtgc aattttacca actaattcat tgtagatata
      541 tacttcaacc aattcaattt ttttcatatc attccatatc ttggtttctt atagtatagt
      601 acctcttttt cttgaacttg atccagtttt taatacatct tcaatacgtt ggtatttttg
      661 ttgagagaaa agactagtga aatcatctac agcatttaat gcaatagcta ttttaactag
      721 attgactaac gagatttcgc ctatgcgttc aaacttacga taagtttcaa tattaacatc
      781 agcacgcaat gctaaacctt tttgagtaag gtttagttct aatctccgtt gtttaacttt
      841 ttgagctata tcaacacata tttcactaac tgacttttgg tttaatagta tcatagtgct
      901 acttaattcg ttaataagtt attaatggta taataatacc attatcaaag atactatatt
      961 gtatcgaaat atacaactat ttacgtattt ttttagctac tgcatactct ttttcctttt
     1021 caaaaatgta gaatataata acgagtttta aacgcttata ctcctcaccc tcgttttcaa
     1081 caatgtatta ttattagaat gcattaaaaa cgctaaaaca aaaatcccct cgctgaaagc
     1141 gaggggaaga taagagtaat gaaacacgtt ttgtttactt gaacgaacgg tctctttctt
     1201 gataaatgcg ttcaatgata tctactactt tcattccttc gagcaagttg gtaccaatcg
     1261 ctcctttggc tttcagcgta tctattacat tttgaataac aaaaccatgg ttttgagcag
     1321 cacctttgta tccaccaaaa tcgttgttta attcaggttg tttcaattct ggcatttcgt
     1381 agtctttaat atggcaatga actacttgat ccatgtattg acctgcaatt ttaacagttc
     1441 ctttttctcc tatgatggta acagaacttt caaggttttt gtcccaaaca gaagtagaat
     1501 agttgagaga acccatacct ccgttaataa attcgaaatc gacgcaacca gtatcttcaa
     1561 attcgattaa atcaccgtgc gagcaattag caaatttacc acgaatattg gtgatatcgc
     1621 caaataacca gtacaacata tctacataat gcgaaaattg cgtaaacaag gttcctccat
     1681 ctaatttgtt agtgccatgc cagctaccac gtttgtagta tttttcgtcg cggttccaaa
     1741 atgcatctac ttttacaaaa taaattttgc caagtgtatc attttcaact aattctttca
     1801 accaagccat tggcggtgaa tagcgatttt gcataacaca aaataccgaa cgagacattt
     1861 ccaaggattt gaatactact ttttcagcat ctgatttgct taatgctatg ggtttttcga
     1921 gcactacgtg tttttttgct tgtaaagctt tgatggcata ttcagcatgg tgaccattga
     1981 ggttacatac atttacaaca tcaatatctg gttgtttagc aagcatttca tcgaaatctg
     2041 aataatacgt ttcagtaatt tctggatcaa ttttatcttt tggtaaaata tcgcatactg
     2101 ctactagttc tgaactagca ttgcgtctta tcatttcggc atgacgtttg cctatgtgac
     2161 ctaatcctac gactgcaaat tttattttat tactcatttt attcgtttta cagtgttatt
     2221 acttaactgg tattcttgtt gcgtttctgg acaaatagct attccttttt catcaaaatt
     2281 aaggcgatga ccatattcac ttacccaccc tatttgacgt gccggattgc ctactaccaa
     2341 tgcgtaagga agaatgtttt ttgtaataac cgcaccagct ccgattaggg caaattcgcc
     2401 tatttcgttg ccacatacaa tagtagaatt agctccaata ctagctcctt tttttatgag
     2461 tgttttagca tattcagctt tgcgattaac gtgactacgg gggttaatta cgtttgtgaa
     2521 taccattgat ggaccaagaa atacatcatc ttcgcatatt acaccagtat aaactgatac
     2581 attgttctgt atttttacat ttctacccaa aattacgtct ggtgatatca ctacattttg
     2641 tccaatattg caactttcgc ctattgtaca gttaggcatg atgtgagaaa aatgccaaat
     2701 tttagtgcca tttcctatct ggcaaccttc atctacataa gctgattcgt gtttaaaata
     2761 tgacatatta ttgattgttt atagcggtta aaaattcaga tgtaatgtaa ttcaattgtt
     2821 cttcgtcaag ttcggtatgc attggcaaac taataacgca ggcacttaat gcttctgcta
     2881 ccggaaaatc gccttctttg tatcgtgcat cttgatatgc tttttgcata tgtaatggca
     2941 ctggataata taccatagct ggaattcctt ttgcttctaa tgctttttgt aaagcagctc
     3001 tatctacatt ttttaatttt aatgtatatt ggtgatacac atgtgttgat tgaggaaatt
     3061 cgtaaggaat agttattttt ggatgattag caaatgcctt gttgtatgct tttgcagcag
     3121 cttgacgatt agctatgtag gtatcaaggt ttttaagttt tacatcaagc acagcagctt
     3181 gaatgctatc taaacgagag ttaacgccta tcatatcgtg gtaataacgc actaccatac
     3241 catggttagc aatagcacgc atagcagctg ctaaggtgtc atcattggta aaaattgcac
     3301 ctccatcacc ataacaacct aaattttttg atggaaagaa agacgtacaa ccaatatttc
     3361 ctatacaacc cgattgtact ttgcgaccgt ctttgaaagt atatacagag ccaatagact
     3421 gacaagcatc ttctactaca aataatttgt gtttatttgc tacttccata attgcatcca
     3481 tattagcaca ttgtccaaac aaatgtacag gaataatagc ttttgttttt ggtgtaattg
     3541 cttttttaag agcatcgata gatatattaa aagtatcttc ttccacatct actacaacag
     3601 gagttaatcc taataaagca ataacttcgg cagtagcaat aaatgtaaag gtaggcgtaa
     3661 taacttcatc acctgctttt aatcccaatg ccattaatgc aatttgtaaa gcgtcagtac
     3721 catttcctac aggaatgacg tgttttacat gtaggtatgt ttctaagttt ttttgaaata
     3781 cattcacttt gcctcctttg atgaaggtag tggtgtcaat tacttcttga attcccgcat
     3841 tgacctcttc tttgattttt tcgtattgac tttttaagtc aaccatttga atctttttca
     3901 tattcattta ttgatattag tatataaaaa taacggttaa aatcctaaac taaatccgat
     3961 agtataggta atattctgac cttgataaaa cgttggcgaa tacatatcta aatacccttg
     4021 tgcatctttt cttatttcag cctcaatagg agtagatgtt acgttgtatc cttttgcgtg
     4081 attgtactca acacttgcaa tggcataagc gttgttccat agttcgtaac ttgctttgct
     4141 tattagtcga ttattactcc acgtaatatc ttgtaataca ggttgactta taacttcttt
     4201 cacacttcgc ctgatgtacg aatattcatt tccatgaagt gcctgaatat aagcaagttg
     4261 tacggatagt ttttgaatgg gtttgtactg caaacttaca aataattctt gagaattatc
     4321 acctaaataa tgacctaaat tatacgaatt tgatgcccat gttaaacttt ctatcgaatg
     4381 cttgtaattt accatagtac tgtaagtaaa ttcagtggta aatgatagat ttgaaatagg
     4441 gaaatttgac aaatggaatc cacctttgta tgatattgga ttagtttctg gatttgaagg
     4501 caagaaacgt gaaaatttta tttcatccac aaacatgcta ccatatagat gtaaatgctt
     4561 aatatttcga gaacttacat taataaaaaa ttgtgagttt tgattttctg ttttaattcc
     4621 ttttgttaat agatggtcaa ttgatttata gaaagcaatt ggtatcaaat atgcgggttg
     4681 tatacttctt tcggcataca caatagcatt acccactgaa atattcaatt tcggaattgg
     4741 ggtgaaagtt agcatattgg cagctacaaa cttattcata gggcgatact gttttttttc
     4801 gccatcaatg gtatgttgct caatatagta ataactgcta tctagtacat tgctaatcag
     4861 aaaaccatgt atgtaattta attcaaacca agatgtgggc tttagtttca ttgtaatcat
     4921 tggaaatgaa ggagctcttc ctgataatat atttgaaccg ttgtagttat ctccccagct
     4981 tacattgtct ttgattaaac caatacttcc ccactcgtta tgcaacgtaa ttcctcctct
     5041 actatcacta aaatcgccac catagtttgc ttctttgtat tcaacaccag gcaaatcgtt
     5101 aaggtaagca ggttgtgata aacgtgctgc atttacaggt aacgaagata agtattctcc
     5161 actgtacgaa ttatcacgca aacttccata aacagcaatg tttttaccaa tattcatata
     5221 catatcagca ccaaaccaac ggtgtgtaat agcaccgtgt cgattactat agatatgcat
     5281 ccctaataat ggtgttattt ttgctgacac caaattgtct ttataatgga ctgcaggttc
     5341 gaccaatgca acattccacg catttttttg tacaagagga aggtgagagg tgggaaaatt
     5401 ttggtaaaaa agtgcgtaat tttgcataaa aaacaccaaa tcaccttttt gacgtttggt
     5461 tagcaaagaa tctgcctttt gtgcttcttg caatttgctg gcaataaaat ttttactgta
     5521 tggttttatg cttgaattta actcaatgat accatcattc gccagttcat ctaaaaagtc
     5581 gtatatgcga gaataagaga tgtgttcagc attggcttgt gcatatgcag tttgtgataa
     5641 aaataaggca aaacagaaaa aaatcaaacg ccaacaaagg gtgcaatttt tctttttatt
     5701 aatttgtatc actatactca tttacttcta gcttttttaa acacaatatt gacaaaaatt
     5761 ttccaaaaat aaatgatatc ggtaatcaat acaccttttt tctcacacat ttgcaaatac
     5821 ctcatttcag aagattcaat ttcttctaat gtttttggca tatcggcata aaatggaggt
     5881 aataatcctg gtttaaattt aatgcgtttt tcttggagct ctttgctata taaactaaag
     5941 tagtgttgac tgattggacg tactccaact aatttcatat cccctttaag gaaattgaga
     6001 atcataggca actcatcaat ccaaaaacga cgaaaaaact tacctaaagt actgatacgt
     6061 atgtcatgac taaacttacc tccttcttgc aatttgttgt gttcgtatat gtaggcttgt
     6121 aaaaattctg agtatggaaa catggtacgc attttataaa acttaaatac acgaccattt
     6181 ttacctatac gttttaattg tatgacagga ccgtagcgac gtttttttct tggattaggt
     6241 tcttgttttc tacgtgcaac aaaaaatagt tgatcgccaa ttttcttttc tccaataatt
     6301 tcgaaaccgc aataatacaa gcgcccatac acttcggttt tcgttaatac acggtttttt
     6361 ccttgagtta tgtcataata taggcggcgg gtaataaata ttttgggaat aacccttttt
     6421 actaaataat aaaatgaata aatcacccaa ttaatacctt tggggtattt ttttagaata
     6481 tcacgttttt taactccact attttcaaaa caacctatat atataccatt gtcagggagt
     6541 ttttcattaa tttttgaaaa caatacatta atacctctta tgtcatttaa tcgtttaagg
     6601 ttgatgattg tatcataacg atacgtacgc agtttctcaa aattaaaaat tgaaccagat
     6661 gaaagcaatt ttgttgctga acttttcagg ttaataacct tacctagata gtccaaaact
     6721 ctcgcatttg tttctttaac gattgataac tcaatggtat gtacagtgtc gttatctagt
     6781 tcatacggtt ctttaactaa tgatgcagga ggacgtattt caataggtgc aacttcttca
     6841 atatccaatg catataaata tgcgaaatgt aacgttgtat gtactaatga caacaacagt
     6901 acgcccaata caaatattga ataattgata atggaaacag gaagaagcag agcgtattca
     6961 aataaagcaa aattaattgt tgctgcaatc actgtagcat aaatgctttt aaatgacttt
     7021 gcaacatatt ctttttctat actatttttt ttgtaatgct taaacaaata atggaatagc
     7081 atccacaaaa ctgaaaaaga gatatagagc ggataagatt gtgctgtaat agtatctact
     7141 gcttttaacc aaaaggttat aagaagaaaa ttcgccaaca atattactac atcaattaag
     7201 atgaatcgta ttccagggcg atataatttt aatgcttttg tcatgaaaat ctgataattt
     7261 tacattttca ctacaaaagt acacgttttt tgttaaagaa agtagatatt cattgctatt
     7321 ttgaagaaaa gtatctaaaa tcagaacttt ctctgttaat tttaacattt aatgatacta
     7381 attgattatt tgggatgata tcttgtttga aaaaattcag ttagacgaaa ctcttcatac
     7441 tagtatacca cttttctgag tagtattttt agaactaaaa actacattga atcaagtgaa
     7501 aagcctttat aagttatagt atatccagtt aatttttttg atagattttg tgctttttct
     7561 tctaaaacac gtaaatcaat tttatcttta ttacgtttaa cttctgcaat aatcgccttc
     7621 ttctcgtatt cgtttagagc aacaatatct atttcatttt ggtttccact ttcccaataa
     7681 gaacctattt gagataatgt catttcattt gttaattttt ccttgaaata acgttctagg
     7741 atttgtccgc tatacgttgt ataatctcgt aaaaacagtt cttttaaata atcaaaactc
     7801 tcagtttcaa ctgccccacg atatttataa ataaaacgaa accaaaaatt cagaaaattg
     7861 tcgttgagct tatacttaac gtttttagtt cctggtttag ccaatattgg tcgtacttta
     7921 gtaattaatg catagtcgtt ttctaatttt tctaaatatc ctcctatgga tatatggagg
     7981 attgattcaa tctccgaacg cgaggttttt gaatttgcaa ttaatgaaag aattgaaaaa
     8041 taattgccat actctttgcc aaactcatca attaatacat ttttcccttc atctaaaaag
     8101 agtgaatttt ctgaaaaaat ttcattcacc atatcttcta gcgtaaaagc acccgcatgg
     8161 actaacattt ctacatactt agctacacca cctgtaaaaa gataaaatgc taataaattt
     8221 tcattattat tttctgatgt atagtcattt aaaattgttt tgattgtttt tatatcaaaa
     8281 gctttcacat gaatacgtgc agttgctctt ccaaacagcg gttcttttgc attttcaaat
     8341 attcttttca ttaacgaata aatagagcca cataatatca agttaatttt actattgtct
     8401 ttatttgcat cccaaacatt ttgaatttca ctatatatac tcgagttaac ggaatgaaaa
     8461 tcttgaaatt cgtcaattat taaggtgaaa tttgtcgttt tagaaagttc cattagatac
     8521 ccaaagatgc ttttgaatgt tctaaattct ccataaactt tgatgtgtaa tttatattga
     8581 atttcttgta taaattcttc gcacagtaaa gcttcattct ttttttcaac aaaaaaatac
     8641 agcattgcgg tttcagtaaa tgcttttttt aacaaagctg tttttcctat ccttctgcgt
     8701 ccaattacaa aagtcatttg ggcaattttt tttgataatt gttcaatttt agttaattgt
     8761 tcaatttctt tttctctgtt gtaaaatttt tgcatagctg taaagtattt aatcaactac
     8821 tatttgtaga tacaaagata taaaaaaaaa aacgaattgc aacccccatt acagtaacag
     8881 cggttgcaat taaacaattt cgtgcgaaaa ctatcctaaa aaactactta tttctttttg
     8941 aaaggatata tttgatggta ttttttaaat tctgaataat gaattcaagt atccattctt
     9001 tgttggaatt tgtccaacgt tgtgggtgtg tggtaatcat tatttggttt attccttctt
     9061 ttgttaacaa accattctca atccctttga tgatatcata cgtggaatgg aatttcagcc
     9121 ccctactatt ccaattatct tgatgcaaag gaactttatc acgaacgctt acattgtaac
     9181 catcccaaca cctacccgta tcagtaaggt aaaagaattg gctaaaatct atatcgtaat
     9241 agggttcgcc aataatgcca taatctttat aatcagcgtg tttccataag tctttgctat
     9301 cccattttga tgttgggttg ccgtgcatac aaatggtttt tacgggataa tatgtgcgga
     9361 aataagccaa ttgttgctga aaatgtgcta tggcttgtac atgattgcca ttaaaaatac
     9421 ccatatcttc gtaatgatag cctatttcat gacccaaagc tgcaatttga gtgataatct
     9481 caggttgatt gctttgtggt actaccctaa aataataact agctactata cccaactctg
     9541 cctcaagctg tgctgtagcc aatgagtttg ctgcttttaa atctacatca tgacgtaaaa
     9601 taacgacttt ttctggcaaa gaggaatttg tttgtttggc aatacaatat tgttcgaatg
     9661 taataaactg ataagcagac tgttgtaatg ctatcagtaa atctttgtat gcttgtaggg
     9721 taaaatcttt catacggttt attttgaata ttgggttttt attgctttta aaaacctaaa
     9781 tgagaagata aaatactcat tataccatga tctaaaacca tgtgtaaatc ttctgttatt
     9841 tgcatgtcat ttactggaat atgtacacca tgatgtgcta tttgttttaa ttttccgcca
     9901 tcatatcctg aaatatcggc agctaccgac gctatacgtg tgcctttacc accagctatt
     9961 attactactt tcatcttaca aaagtctata tttgtttaca aagatacaag atattattta
    10021 atataggaat gcgccctgca aaagaaacgt tatttaaact gctgctgaat atctggattt
    10081 agtctaagtt gttgaatact tttaggatac tctaatacca cccacattaa aaaagcggtc
    10141 acatctattt tatcagccaa caattgagct ctatttttac gcttatactc ctggctagcg
    10201 ttatgtttta atacatccac gcccttttcg atagctaata attggtcgga ttcagattca
    10261 gaataattaa aacacaaatt gtatttttga gcaatatcgc gcgtataaaa tcggcctgtg
    10321 ttatcgatat aaacacctgg tacgcccaac atagcacctt cggccaccat cgtggcactt
    10381 tca
//
LOCUS       DAOSRP010000067        10358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6278_length_10358_cov_5.465787, whole genome shotgun sequence.
ACCESSION   DAOSRP010000067 DAOSRP010000000
VERSION     DAOSRP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6278_length_10358_cov_5.465787"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1252
                     /gene="hisC"
                     /locus_tag="PLJ82_05420"
     CDS             218..1252
                     /gene="hisC"
                     /locus_tag="PLJ82_05420"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOR40980.1"
                     /translation="MNVENLVRKNILALKPYSCARDEFQGEASVYLDANENPMNAPYN
                     RYPDPLQWKLKEKIAKLKSVNPSQIMLGNGSDEPIDLVIRIFCEPLVDNIVAIDPTYG
                     MYKVCADVNNVEYRKVLLNDDYSLDATRVMNAVDKHTKIIFLCSPNNPTGNLLNRNEM
                     KHIIQNFDGIVVIDEAYVDFSPEQSWLVEINTYQNIIVLQTFSKAWGLASVRCGMAFA
                     SEKIISFFNKVKYPYNINILTQQFVEEQLENTDRKEEWVTMLLGQRTYLVEQLNKLPL
                     IQTIYQTDANFVLVKVEDANAIYRYLVDLGIIVRNRNTVSLCLGCLRITIGTQQENDT
                     LLQALKAYTY"
     gene            1264..2400
                     /gene="hisB"
                     /locus_tag="PLJ82_05425"
     CDS             1264..2400
                     /gene="hisB"
                     /locus_tag="PLJ82_05425"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOR40981.1"
                     /translation="MDNLKKALFIDRDGTIIAEPPVTFQVDNLQQLEFLPSVIRNLYL
                     IRKHTDFEFVMVTNQDGLGTEVYPQENFDSVQAKMLQVLQNEGVVFDDILIDTSFPEQ
                     NLPTRKPQTGLLTSYIAGGYDLQHSFVIGDRITDVELAKNIGCKAIFLQNIENATSIL
                     EEHKLADYCALISTDWDEIAAYLVAKERTATVQRTTKETDILVYVNLDGKGKTSIHTG
                     LGFFDHMLDQIGKHSGMDLTIHVKGDLQVDEHHTIEDTALALGEALLVALGNKRGVER
                     YGYCLPMDECLCQVALDFGGRPWLVWDATFTREKVGDMPTEMFMHFFKSLSDTAKMNL
                     NIKAEGANEHHKIEGIFKALARAIKMAIKRDVFNGELPSTKGVL"
     gene            2424..3194
                     /gene="kdsB"
                     /locus_tag="PLJ82_05430"
     CDS             2424..3194
                     /gene="kdsB"
                     /locus_tag="PLJ82_05430"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOR40982.1"
                     /translation="MNTLRFLAIIPARYASTRFPGKPLAILDGKTMIQRVYERVSSEI
                     QDVYVATDDERIALEVARFGGKYVMTSPLHTSGTDRCYEAFTKIRGDFDVVINVQGDE
                     PFIHPEQMSLLKTCFDDSKTEIATLVKPYSYDDIVDNLATPNTPKVVITKDNYALYFS
                     RSIIPYKRGTDRHIWLQTHTYYKHIGMYAYKHTVLKEITQLEPSSLEKAESLEQLRWL
                     ENGYRIKVAITEHETIGIDTPDDLAQALTFLKNQKRID"
     gene            complement(3195..3899)
                     /locus_tag="PLJ82_05435"
     CDS             complement(3195..3899)
                     /locus_tag="PLJ82_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOR40983.1"
                     /translation="MSAKLYLLPTTLGESEINAVLPSTNNEICSQLKHYIVEDVRTAR
                     RFLKKVDKTIDIDSIQFYVLNKHTTAEELSTFLKPLVAGFDMGVLSEAGCPAIADPGA
                     DVVRMAQEKNIQVVPLVGPSSIILSLMASGFNGQNFAFNGYLPIQDDERIAMLKKLEK
                     KVYNEQQTQIFIETPYRNMKLVADIVQHCMPNTKLCIATDITLESEYIKTKTNAEWKK
                     GIPDLHKRPCIFLLYK"
     gene            3963..5327
                     /locus_tag="PLJ82_05440"
     CDS             3963..5327
                     /locus_tag="PLJ82_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOR40984.1"
                     /translation="MQLPVDFIHGMKGLLGESDYSLFFEALQQEPPVSIRLNTKFTSA
                     QVKEYQSVSWCKNGYYLPSRPLFTADPLFHAGAYYVQEASSMFIEQIIKQHVQHDVIA
                     LDLCAAPGGKSTHLASLLSTNSLLVSNEFIRSRANILAENCMKWGNGNMIITSNSSDE
                     IAQLESFFDFILVDAPCSGEGMFRKDVNAINEWSLLNVEMCVTRQRHILKQAWQALAE
                     DGFLVYSTCTFNEKENEENIKWLIDELGAEYIPIGMDNFPEITNTDVGYRFYPHKTKG
                     EGLFMALVQKKSACNKQNIKLKTDKNKQNIADIEALNLYINDSSDWIIQQNNQQYRAI
                     LASKQQEIKWIEKHLYVLFSGIQLAEKKGNDFVPHEHLALSKQLNTNSFQVVDVDWIT
                     AIQFLKREALFLPDASKGYVLLQYNNVPLGWVKNIGNRCNNMYPSSWKVRMDISHFTN
                     IQII"
     gene            5334..6080
                     /locus_tag="PLJ82_05445"
     CDS             5334..6080
                     /locus_tag="PLJ82_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR40985.1"
                     /translation="MEVIFLGTGTSTGVPYINCPCDVCQSTDSKDKRLRSSVLVSVDT
                     KEILIDCGPDFRQQAIVNDISTISAVLLTHEHFDHVTGIDDLRAYGEVEIYAEERVCQ
                     SLKRTIPYCFGEIKYPGVPLISLHEIDEMPFDLHNTEIVPIRIWHHKLPILGFRIGAF
                     AYLTDISGIDDKEYEKLQNLDVLVVDALRLKPHFSHFTLEQAISFAEKTNAKEVYFTH
                     ISHQLGKHDEIQSLLPHNMMLAYDGLKITI"
     gene            6215..6526
                     /gene="rplU"
                     /locus_tag="PLJ82_05450"
     CDS             6215..6526
                     /gene="rplU"
                     /locus_tag="PLJ82_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOR40986.1"
                     /translation="MYAIVEIAGQQFKVEKDRTLFVHRLNEERGATVEFDKVLLIDAD
                     GKVKVGAPTIKGAKVLAEVISHVKGEKVIIFKKKRRKGYRVRNGHRQCFTEIMIKELV
                     A"
     gene            6546..6809
                     /gene="rpmA"
                     /locus_tag="PLJ82_05455"
     CDS             6546..6809
                     /gene="rpmA"
                     /locus_tag="PLJ82_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOR40987.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIFGGQYAKAGSIIIRQRGTV
                     HHPGENVGIGKDHTLFALIEGTVVFRRKKDNRSFVSVQAIEQN"
     gene            6902..8173
                     /gene="serS"
                     /locus_tag="PLJ82_05460"
     CDS             6902..8173
                     /gene="serS"
                     /locus_tag="PLJ82_05460"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOR40988.1"
                     /translation="MLTTKFITENATEVIARLAKKHFDASLIVSEVISLDTLRKTTQT
                     KLDEVLAEMNAISKAIGDLYKQGKKQEADEAKEKTVVLKEKSKELSDILDSAEQKLHT
                     LLLQIPNLPHASVPEGKGADDNTIERSGGTIPSLPANALPHWELAKKYDLIDFELGVK
                     ISGAGFPVYKGKGAQLQRALINFFLSNASQAGYLEIQPPYVVNAASGYGTGQLPDKDG
                     QMYHATIDDLYLIPTAEVPVTNIYRDVILQEKELPIKNTAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDKSYETLDEMVNYVQTLVEKLELPWRILRLCGGDMSFTSA
                     LTFDFEVFSAAQNRWLEVSSVSNFESFQANRLKCRYKGDDKKTSLCHTLNGSALALPR
                     IVAALLENNQTEEGIVIPKALVPFTGFTIIS"
     gene            8345..9418
                     /gene="corA"
                     /locus_tag="PLJ82_05465"
     CDS             8345..9418
                     /gene="corA"
                     /locus_tag="PLJ82_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOR40989.1"
                     /translation="METLKPLRSVSILNRKKNDPSNYIFTGNSSTKNINIQLFTYNEE
                     ECVENKIMLLDSIPTFEKNKKNYWLNIHGLNNPENIASICEQQGIHNLVIQDILDVNQ
                     RPKFQEFEHFSFLTLKSIVPSEKNIITEQISFVFGTNFLLSFQERKADFFEHHRYRLR
                     ESKGKLRERGADYLLYAMLESTLDNYFKTINKLDEEVEKLNFSDTTKEPSPNALAIIE
                     GHKKFIHYIKKSILPIKEFTQIVERGDCNYIEQRHVKYFLEIKDLCLTLIDSCDMILA
                     SLESATNLFFSVQGHRMNQVMKTLTIVATIFMPLTFIAGIYGMNFVHMPELEWKYGYY
                     GIWGIMIVLFCAMVLYFRAKKWF"
     gene            9691..>10358
                     /locus_tag="PLJ82_05470"
     CDS             9691..>10358
                     /locus_tag="PLJ82_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR40990.1"
                     /translation="MSLQKDILELREKKAIYEKGGGDTAIEKQMAMGKLTARQRILAL
                     LDEDSFQEYDLFVEHEERNFGMDKKTLHGDGVITGIGTMFGEPVCIYAQDFTVMGGSL
                     GLKHARKITKIMDHALKMKVPCIGINDSGGARIQEGIGALAGYGEIFYRNTLASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPGVIESVLGEVISQEELGGARI
                     HAEIT"
BASE COUNT         3530 a         1571 c         1945 g         3312 t
ORIGIN      
        1 tagagcacat cccttttaag gatggggtcc tgggttcgag ccccaggcgg atcacaaaaa
       61 gaatagccta gcaatatgtg ttgctaggct atttcttttt gaacgaatac taataattaa
      121 atatattgaa gcttcatgat tattgaataa aatacctcaa gaaatgaaca accaatatag
      181 aaatttacta tttttgttgt tcatttatct atttactatg aacgtagaaa atttagtcag
      241 aaaaaatatc ttagcattaa aaccgtattc ctgtgcacgt gatgaatttc aaggagaagc
      301 atctgtatat ttagatgcca acgaaaatcc aatgaatgca ccgtacaata ggtatccaga
      361 ccccttgcaa tggaagttaa aagaaaagat tgcaaaacta aaatcggtta atccatcaca
      421 aataatgctg ggcaatggta gcgacgaacc cattgattta gttattcgta ttttttgcga
      481 acccttagtc gataatattg tagctatcga tcctacgtat ggtatgtaca aagtgtgtgc
      541 tgatgttaat aatgtggaat accgtaaagt gctattgaat gatgattatt cgttggatgc
      601 aacaagggta atgaatgctg ttgataagca tactaaaatt atttttcttt gttcaccaaa
      661 taatccgacg ggtaatctgt tgaatcgcaa tgagatgaaa cacatcatac aaaactttga
      721 tggaatcgtg gtaattgatg aagcttatgt tgatttttct ccagaacagt catggctagt
      781 tgagataaat acgtatcaaa atatcattgt gttacaaaca ttctcaaaag cgtggggctt
      841 ggcttctgtg cggtgtggaa tggcttttgc ttctgaaaaa attatctctt ttttcaataa
      901 agttaagtat ccctacaata tcaatatatt aactcaacaa tttgttgaag agcaacttga
      961 aaatacagac cgaaaagaag aatgggtaac tatgctgtta ggacaacgta cttatctagt
     1021 ggaacaatta aacaaactcc ctctcataca aacaatatac caaaccgatg ccaattttgt
     1081 gttggtaaaa gtagaagatg ctaatgccat ttatcgttat ttagttgatt taggtatcat
     1141 tgttcgaaat agaaacacgg tttcattgtg tttaggatgt ttgcgaataa caattggaac
     1201 acaacaagaa aatgacacac tattacaagc tttaaaagca tatacatact aattctataa
     1261 cgaatggata atctgaaaaa agcccttttt atcgatagag atggtactat cattgcagag
     1321 cctcctgtta catttcaagt agataatcta caacaattgg agtttttgcc atcagtaatt
     1381 cgtaatttgt atcttatacg caaacatact gattttgagt ttgttatggt aaccaatcag
     1441 gatgggcttg gaacagaggt ttatcctcaa gaaaattttg attcggtaca agcaaaaatg
     1501 ttgcaagtat tacaaaatga gggagttgta ttcgacgata ttttgattga tacttctttt
     1561 cctgagcaaa atttgcctac acgaaaaccc caaactggat tgttaacatc ctacattgct
     1621 ggaggttacg atttacaaca ttcttttgta attggagata gaattacaga tgtagaattg
     1681 gcaaaaaata tcggttgtaa agcaattttt ttgcaaaaca tagaaaatgc aacaagcata
     1741 ttggaggagc ataaactagc agattactgt gctttaatat caacggattg ggacgaaatt
     1801 gctgcatatt tagttgcaaa agaacgcacc gctactgtgc aacgaaccac caaagaaaca
     1861 gatattttag tgtatgttaa tttagatggc aaaggtaaaa cttctattca tactggttta
     1921 ggttttttcg atcatatgtt agaccaaatt ggtaaacatt cgggaatgga tcttaccata
     1981 cacgtaaaag gagatttgca agttgatgag catcatacca tagaagatac tgctttagct
     2041 ttgggagaag cccttttagt agctttagga aacaaaagag gtgtagagcg ttatggctat
     2101 tgtttgccca tggacgaatg cttatgtcaa gttgctttgg attttggtgg acgaccctgg
     2161 ttggtatggg atgctacttt tacaagagag aaagtaggag atatgccaac agaaatgttt
     2221 atgcactttt ttaaatcatt gagcgatact gctaaaatga atttgaatat aaaagctgaa
     2281 ggagccaacg aacatcataa aattgaagga atatttaaag cccttgctcg tgctattaaa
     2341 atggctatta aaagggatgt ttttaatgga gaattgccta gtacgaaagg tgttttataa
     2401 ttatttttta taatacttca aaaatgaata cattacgttt cctcgctatc atcccagcac
     2461 gttatgcatc aacacgtttt cctggcaagc ctctggctat acttgatgga aaaaccatga
     2521 ttcaacgcgt atacgaacga gtttcttccg aaatacaaga tgtgtatgtg gctactgatg
     2581 atgagcgtat tgcattggag gtggcacgtt ttggaggtaa atatgtaatg acaagtccgt
     2641 tacacacaag cggtacagat aggtgttacg aggcttttac aaaaattagg ggtgattttg
     2701 atgttgttat taatgtgcaa ggagatgagc cttttattca tcctgaacaa atgagtttac
     2761 ttaaaacttg ttttgatgac tccaaaaccg aaatagccac cttagttaaa ccgtattcgt
     2821 acgatgatat tgtagataat ttagcaacac ctaatactcc aaaagtggtt attacgaaag
     2881 ataattacgc gttgtatttt agtcgttcga ttattccgta taaaagagga acagatagac
     2941 atatttggtt acaaactcac acctattata aacacatagg catgtacgct tataaacata
     3001 ctgttttaaa ggaaattact caactagagc catcatcact cgaaaaagca gagtcgctag
     3061 agcaactgcg ttggttggag aacgggtatc gtattaaagt ggcaataact gaacacgaaa
     3121 caataggaat tgatacacct gatgatctgg cacaagctct tacatttttg aaaaatcaaa
     3181 aaagaataga ctaattactt gtaaagcaga aatatacaag gacgcttgtg taaatcagga
     3241 ataccttttt tccattcagc gtttgttttt gttttgatat attcgctttc taatgtgatg
     3301 tccgtagcaa tgcataattt cgtgtttggc atacaatgtt gtacaatgtc tgcaactaat
     3361 ttcatattgc gataaggagt ttcgataaaa atctgtgttt gttgctcgtt gtacactttt
     3421 ttctctaatt ttttaagcat tgcaatacgc tcatcatctt gaatgggaag atagccatta
     3481 aatgcaaaat tctgtccatt aaatcctgat gccatgagcg ataaaataat agaagaaggt
     3541 ccaactaatg gaactacttg tatatttttt tcttgagcca tacgtaccac atctgcacct
     3601 gggtcggcaa ttgcaggaca accagcctct gataataccc ccatatcgaa tcctgctact
     3661 aagggcttca aaaatgtgct aagctcttca gcagtagtgt gtttgttcaa tacgtaaaat
     3721 tgaatgctat caatatcaat ggttttatcc actttcttta aaaatcgtcg ggctgttcga
     3781 acatcctcta ctatatagtg ttttagttgc gagcaaattt cattgtttgt agagggtagt
     3841 acagcattga tttctgattc gccaagggtt gttggtaata ggtataattt tgcagacata
     3901 tagttggtat tttctgcaaa attactaaaa aaattgtact tttgtgtact taataatgaa
     3961 ctatgcaact tcctgtagat tttattcatg gtatgaaagg cttgctaggc gagtctgact
     4021 attcattatt ttttgaggct ttacaacagg aacctcccgt ttcaattcga ttaaatacca
     4081 aatttacatc tgcacaagta aaagaatacc aatcagtaag ttggtgtaaa aatggatatt
     4141 atttaccttc acgaccttta tttacagctg atccattgtt tcatgcagga gcttattatg
     4201 tacaagaggc aagttctatg ttcatagaac aaattattaa acaacacgta caacacgatg
     4261 taatagcctt ggatttgtgt gctgctccag gagggaaaag tacacattta gcttctttgc
     4321 tctcaacaaa tagtttgttg gtaagcaacg agtttatccg ttcaagagcc aatattttag
     4381 cagaaaattg tatgaagtgg ggaaatggaa atatgattat tacctccaat tcttctgatg
     4441 aaattgctca attagaaagt ttttttgatt ttattttggt agatgcccct tgctctggcg
     4501 aaggtatgtt tcgcaaagat gtaaatgcca tcaatgagtg gtcgttgcta aatgtagaaa
     4561 tgtgtgtaac tcggcaacgg catattttaa aacaagcgtg gcaagcatta gcagaagatg
     4621 gttttttggt ttacagcacc tgtaccttta atgaaaaaga aaacgaagaa aatataaaat
     4681 ggttgataga cgaattgggt gctgaatata taccgattgg catggataac ttccctgaaa
     4741 taacaaatac agatgttggt tatcgttttt atccacacaa aacaaaagga gaaggcttgt
     4801 ttatggcatt agttcaaaaa aagagtgctt gtaataaaca aaatataaaa ctaaaaacag
     4861 acaagaataa acaaaatatt gctgatattg aggctcttaa tttatacata aacgattcgt
     4921 cagattggat aatacagcaa aataaccaac agtacagagc aatattagcc tccaaacaac
     4981 aagaaattaa atggattgaa aaacacttat atgtattgtt ttctggaatt caactggctg
     5041 aaaaaaaagg caatgacttt gttcctcacg agcacttagc cctttctaaa caacttaata
     5101 ctaattcttt tcaagtagta gatgttgatt ggataacagc cattcaattt cttaaacgag
     5161 aagccttgtt tttacccgac gcatcaaaag gttatgtgtt gttgcaatac aataatgtac
     5221 cattaggatg ggtcaaaaat ataggcaaca ggtgtaataa tatgtatcca agttcgtgga
     5281 aagtacgtat ggatatttca cattttacaa acattcaaat catataaacc ataatggaag
     5341 tcatattttt aggaacaggc acatctactg gtgttccata tattaattgt ccttgcgatg
     5401 tatgtcaatc tactgatagt aaagataagc gattacgatc ctctgtttta gtttcagtcg
     5461 atacgaaaga aatattgata gattgtggac ctgattttcg tcaacaagcc attgttaatg
     5521 atatttccac aatatcagct gttctgctta cacacgagca ttttgaccac gttactggca
     5581 tagatgattt acgagcctat ggcgaagtag aaatttatgc agaagagcga gtgtgtcaat
     5641 cattaaaacg aaccataccg tattgttttg gcgaaattaa atacccagga gttcctctta
     5701 tttcgttaca cgaaattgat gaaatgcctt ttgatttaca caatacggag attgttccga
     5761 tacgtatttg gcatcataaa ttacctattt taggttttag aataggtgct tttgcttatt
     5821 taaccgacat tagtgggatc gatgacaaag aatacgaaaa actccaaaat ttggatgtat
     5881 tagtagtaga tgcactacgc cttaaaccgc atttttctca ctttacctta gaacaagcca
     5941 tcagttttgc agaaaaaaca aatgctaaag aagtgtattt tacacacatt agccatcagt
     6001 tgggcaaaca tgatgagatt caaagtttac ttcctcacaa tatgatgttg gcatacgatg
     6061 ggttgaaaat cactatatga cacaaaaaag aatataaatt acttgtacag aataaaatta
     6121 tatagtatct ttgccactca aattctgaaa ggtagaaaag aaatgcagct aaagcattca
     6181 cctcacgaag gtattttata attaatttat aactatgtac gctattgtag aaattgcggg
     6241 acaacaattt aaagttgaaa aagacagaac attgtttgtt catcgcttaa acgaagaaag
     6301 aggtgcaact gtagaatttg acaaagttct attgattgat gctgatggaa aagtaaaagt
     6361 tggtgctcca actataaaag gagcaaaggt attggcagag gttatttctc atgtaaaagg
     6421 agaaaaagta atcattttca aaaagaaaag aagaaaaggt tatagagtaa gaaacggtca
     6481 tcgccaatgt tttacagaga ttatgattaa agaactagtt gcttaacaaa taaaaattta
     6541 cagaaatggc acataagaaa ggagtcggta gttcaaagaa tggacgtgaa tcggaaagta
     6601 aacgacttgg agtgaaaata tttggaggtc aatatgctaa ggcaggtagt atcattatca
     6661 gacaacgtgg tacagttcat cacccaggtg agaatgtagg tatcggtaaa gatcatacat
     6721 tatttgcttt aatcgaaggt actgttgttt ttagaagaaa aaaagacaat cgctcttttg
     6781 tttctgttca agcaatcgaa caaaactaag tttagaaaac tatatttata aaaaccctcc
     6841 tggattcttc ttgaagaaaa gggtgggttt ttttttattt acttttataa tagcacaact
     6901 tatgctcact accaagttta ttacagaaaa tgccacagaa gttattgctc gtttggcaaa
     6961 aaaacatttt gatgcatcgt taatagtatc tgaagtaatt tctttagata cattacgtaa
     7021 aacaacacaa acaaaattag acgaagtatt agccgaaatg aatgctatct ccaaagcaat
     7081 tggagatttg tacaaacaag gcaaaaaaca ggaggcggat gaggctaaag aaaagaccgt
     7141 tgtgttaaaa gaaaaaagca aagagttaag tgatattttg gatagtgctg aacaaaagtt
     7201 acatacacta ttattacaaa tacctaattt gcctcatgca tctgtacctg aaggaaaagg
     7261 agctgacgat aatactattg agcgtagtgg tggcacaatt ccttctttac cagcaaatgc
     7321 tttgcctcat tgggaattag caaaaaaata cgatctgatt gactttgagt taggagttaa
     7381 aatatcagga gcaggtttcc ctgtttataa aggaaaaggg gcacaattgc aacgtgcttt
     7441 gataaatttc tttttatcta atgcctctca agcaggttat ctcgaaatac aacctccgta
     7501 tgtggtgaat gcagcatcgg gttatggaac tggtcaatta cctgataagg atgggcaaat
     7561 gtatcatgca acgattgatg atttgtattt aattccaact gcagaagtgc cagtaaccaa
     7621 tatttatcgc gatgtgattt tgcaagaaaa ggaattgcca ataaaaaata cggcatattc
     7681 agcttgcttt agaagagaag cggggtctta tggaaaagat gtgcgtggat taaatcgctt
     7741 acaccaattt gataaggttg aaatagtgca aattcaacat ccagataaat cctatgaaac
     7801 attggatgaa atggttaatt atgtacaaac attggttgaa aaacttgaat tgccttggcg
     7861 tatattacgc ctttgtggtg gagatatgag ttttacttcg gcattaactt tcgactttga
     7921 ggtattttcg gctgctcaaa accgttggtt agaagtaagt tctgtatcca actttgaaag
     7981 ttttcaagca aatagattaa aatgtaggta caaaggagat gataaaaaaa ctagtttatg
     8041 tcatacactt aatggtagtg cacttgcgtt gcctcgtatc gtagctgcat tgttagaaaa
     8101 taaccaaact gaagagggta ttgttattcc aaaagcctta gttccattta ctggttttac
     8161 tattatttcg taacaaatac tcatttctta ctgtatattt aaaataggag gctttttgtt
     8221 tcctattttt tttgttcaga catttaatgg cacatttacg atgaaaatat aaaaaatgta
     8281 gttatcttcg aagtctataa gtaaaaaatg tatgtaaaga atatataagt aactagcaaa
     8341 aaacatggaa actctcaaac cattacgatc ggtttctatt ttaaatagaa agaaaaacga
     8401 cccaagtaac tatattttta caggaaattc gagtactaaa aatattaata ttcagttatt
     8461 tacttataat gaggaagaat gtgtagaaaa taaaattatg ctactagata gtattccgac
     8521 gtttgaaaaa aataaaaaaa attactggtt aaatatacat ggattaaata atccagaaaa
     8581 tattgcgtct atttgtgaac aacaagggat tcataaccta gttattcagg atattttaga
     8641 tgtaaatcaa cgaccaaaat ttcaggaatt tgaacatttt tcttttttaa cattaaaatc
     8701 aatagttcct tcggagaaaa atatcataac tgagcaaatt agctttgtat ttggtaccaa
     8761 ctttctattg tcttttcaag agagaaaagc ggactttttt gagcatcatc gatatagact
     8821 cagagaaagt aaaggcaaat tacgagaacg aggtgcagat tatttgcttt atgctatgct
     8881 agaatcaact ctcgataact atttcaaaac cattaataag ctagatgagg aagtggaaaa
     8941 attaaatttt tcggatacca caaaagaacc atcccccaat gctcttgcta taattgaagg
     9001 acataaaaaa tttattcatt atataaaaaa atccatatta cccataaaag aatttacaca
     9061 aattgttgaa cgtggtgatt gtaactatat agaacaaagg cacgtaaaat attttcttga
     9121 aattaaagat ttgtgtttaa cattgattga tagttgcgat atgattcttg cttcgcttga
     9181 aagtgcaaca aacttatttt tctcagttca ggggcatcgt atgaatcaag ttatgaaaac
     9241 cttaaccata gtggctacaa tctttatgcc acttacattt attgcaggta tttatggtat
     9301 gaattttgtg catatgccag aacttgagtg gaagtacgga tattatggca tttggggtat
     9361 tatgattgtt ttattttgtg ctatggtact ctattttaga gctaaaaagt ggttttagat
     9421 atatcttttt atacttacgt aatttgttat tcttcattcg cgtattttaa attttatctt
     9481 cttaaaaact tttgtttttt gtttcttttt taatttttaa gggttaattt acacatttta
     9541 tacttttttg tgtattgtaa gtatttgata atcaatgata gatttttttt gttgatattt
     9601 ttatctactt tatcccgttc acaaataaaa gatttgttta actttgccat gtgaattaaa
     9661 atttcaatta ttcattaaaa agtaacaagt atgtcactac aaaaagacat tttagaatta
     9721 cgtgagaaaa aagccatcta tgaaaaaggt ggtggcgaca cagctatcga aaaacaaatg
     9781 gctatgggga aattaacagc ccgccaacgt attttagctc tattggatga agattctttt
     9841 caagagtatg atttgtttgt tgagcacgaa gagcgtaatt ttggcatgga taaaaaaaca
     9901 ctccatggtg atggtgttat tacaggtatt ggtaccatgt ttggtgagcc tgtttgtatt
     9961 tatgcacaag attttacagt aatgggaggt tctttgggtt taaaacatgc tcgtaagatt
    10021 accaaaatta tggatcatgc attgaaaatg aaagttcctt gcataggtat taacgattca
    10081 ggtggagctc gtattcaaga aggtattggt gcattagcag gatatggtga gattttttat
    10141 cgcaatacat tagcttctgg tgtaatccct cagatttctg taattttagg accatgtgca
    10201 ggtggagcag tgtattcgcc agctcttact gattttgtat ttgtggttga aaatatttca
    10261 aagatgttta tcacaggacc aggtgttatt gagtctgtgt taggtgaagt tatttcacaa
    10321 gaagaacttg gtggagcacg tattcatgcc gaaattac
//
LOCUS       DAOSRP010000068        10147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6428_length_10147_cov_4.961157, whole genome shotgun sequence.
ACCESSION   DAOSRP010000068 DAOSRP010000000
VERSION     DAOSRP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_10147_cov_4.961157"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLJ82_05475"
     CDS             complement(<1..783)
                     /locus_tag="PLJ82_05475"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOR40991.1"
                     /translation="MCRIEKIVAREVLDSRGNPTVEVDVTLESGIVGRAAVPSGASTG
                     ENEAIELRDGDKGRYLGKGVLKAVANVNNVIAPELIGMCAMEQRAIDKKMIALDGTKT
                     KSKLGANAILGVSLAVAKAAAEYLGLPLYRYIGGTNAFTLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAKSFREGLRMGAEVFHSLKKVLHDKGLSTAVGDEGGFAPNLAGGTEE
                     ALESILTAVKNAGYVPGKDVTIGLDCAASEFYKNGIYDYSKFE"
     gene            complement(937..1749)
                     /locus_tag="PLJ82_05480"
     CDS             complement(937..1749)
                     /locus_tag="PLJ82_05480"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOR40992.1"
                     /translation="MLAKINETATYLKGKMQRFPKVGIILGTGLGELASQITEKQEIE
                     YQTIPNFPVSTVEGHSGKLIIGKLGTTEVLAMQGRFHYYEGYSMQEVTFPVRVMKALG
                     IETLLVSNASGGVNPSFEIGDLMIITDHINTFPEHPLRGKNHPELGVRFPDMSQAYSK
                     ELISKAKKIAARNTIKVVEGVYLGTSGPTFETPAEYVYFRNIGADTVGMSTVPEVIVA
                     VHSGIKVFAMSIITDLGVPGKIVEVSHEEVQHIANQVQPKMTQIMKELIAEL"
     gene            complement(1688..2782)
                     /gene="lpxK"
                     /locus_tag="PLJ82_05485"
     CDS             complement(1688..2782)
                     /gene="lpxK"
                     /locus_tag="PLJ82_05485"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOR40993.1"
                     /translation="MLLWPISWLYGISMSIRNMLFDKHILPTESFPISVISVGNITVG
                     GTGKTPHTEYLVSILKKKFNVAVLSRGYKRKTVGYIYANATASAQTIGDEPFQIYQKH
                     PDIVVAVDANRRNGIKQILLSHPQTDVVLLDDAFQHRYVEANQTILLVDYNRPITEDN
                     MLPYGNLRESIDGIKRADIILITKCPPNLTPIEARNIHKSLNLLAYQDIFYTSFAYGD
                     VKSVFTSNQIVTTSIIQEQQFGILLVTGIAQPEPLLDYICTLTPYVEKLLFPDHHFFT
                     QQDMGIIIKMFENMKQEKRLIMVTEKDAARLVNDPLFPDHLKPYTCMLPISVNFLFQQ
                     QKIFNSKIIDYVSKNQRNSNVPKRKNATLS"
     gene            complement(2806..4557)
                     /gene="sppA"
                     /locus_tag="PLJ82_05490"
     CDS             complement(2806..4557)
                     /gene="sppA"
                     /locus_tag="PLJ82_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOR40994.1"
                     /translation="MKQFLKFTFASCLGFLLSFFTICIIVAIVIVATVTGFSSKQITV
                     APNSVLKIEFNGVLKEQARDMSFFDAWSSTNITELGIHDIIKSIQHAKTDENIVGIWL
                     QMDLFSAGNASISEIRDALVDFKKSGKFIIAYGDNYTQQLYYLATVADKIFIHPSGTI
                     ELKGLASKPIFFKNALQKVGVDMQVVKVGDYKGAPEMYTNNAMSDENKAQTKNIINQL
                     WRKMCTNIASERNISIEQIESLVEKSVMFAPTKSYVTEGLVDSLLYPDELVSYFKTHI
                     DSTYNEEKQMVSYGDYIVLASQNKSNNNKIAVLYANGGIDDGDIEGIDSEELSKLLSE
                     TRKDSSIKAVVLRINSPGGSAFGAERIWRETSLFKGVKPLVVSMGDYAASGGYYIATQ
                     ADSIFSNTETLTGSIGIFGIIPNFSKISEKFGITYDPQTTHTLADFPSFTRDMTTYER
                     NILERYITDNYQLFLHRCSEGRSITKAALLPLAGGRVWTGSDALTHGLVDKIGGLQQA
                     IQSAARMASITSYEVEHLPKKKTVWESMLEGTTIQIQSLLTKNIPLHEYRLIRSFIDN
                     KTRIIIQARMEENSEIW"
     gene            4780..5799
                     /locus_tag="PLJ82_05495"
     CDS             4780..5799
                     /locus_tag="PLJ82_05495"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOR40995.1"
                     /translation="MEEINWSNLSFSYMPTDCNIRCSYQNGEWGELRKHTDVYLNMHM
                     AATCLHYGQECFEGLKAFRGKDGKIRVFRIDENALRMQSSCEGILMAKFPVDKFKEAV
                     KLVVKENERFVPPYESGASLYIRPLIIGTSAQVGVNAAKEYEFILFVTPVGPYFKGGF
                     QTTPFVILRQFDRTAPLGTGMYKVGGNYAASLVAVDKAHKMGYSNVFYLDAKEKKYID
                     ECGAANFFGIKNNTYITPKSTSILPSITNKSLMVLAQELGLKVEQRPVPEEELATFEE
                     AGACGTAAVISPIQRIDDVEEGKSYVFSADGKPGKVCEQLYTKLRAIQYGDEPDKYGW
                     ITVIE"
     gene            5841..6254
                     /gene="aroQ"
                     /locus_tag="PLJ82_05500"
     CDS             5841..6254
                     /gene="aroQ"
                     /locus_tag="PLJ82_05500"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOR40996.1"
                     /translation="MQLVIINGPNLNLLGKREPQVYGNQTFEEMLAEIRKAFPSIDIL
                     YFQSNVEGEIINKIHEVGFTADGIIINAGAYTHTSIAIADAVKSIKTPVVEVHISNVF
                     ARESYRHESFLTPVCKGCISGFGIKSYYLAIQSFF"
     gene            6331..7344
                     /locus_tag="PLJ82_05505"
     CDS             6331..7344
                     /locus_tag="PLJ82_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR40997.1"
                     /translation="MKYKYVLSLLWLFFVVFIIRAQDGKIIGQVFDNTSKKPLDFANV
                     ALFTNQSKEPLQAILSDSEGKFILQNLPYGSFTLKVSFVGYESFETSVLVSKEKPTAR
                     VNKVVLKENAQQISEVQVVGQRSGVSFEIDKKVFNVDETALAQGSSTTDVLKNIPSVA
                     VDLEGNVSLRNNSSVEIWINGKPSGLTDENRGQVLEQMPAETVEKIEVITNPSARFNP
                     EGSAGIINIVLKEKNRGSYLASINSNIGYNESGAINGSFGGNYMYNGPKFDVQLNGSI
                     RGGERKNASYNNRATLVAEDTISHLNQRSLSESDMFMGFMRGGVTYHIDKKMILDLVV
                     LVC"
     gene            7338..8771
                     /locus_tag="PLJ82_05510"
     CDS             7338..8771
                     /locus_tag="PLJ82_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOR40998.1"
                     /translation="MLGDFSGNSTINYHSLDANRDTLETRDRLADNANNRMHYNVSLD
                     YKHFFDKTNHELSSSIYYVRGNHNSFNAYNTSRFGNDGVYINDSKTYQEQQATRNMYN
                     ITWQADYFNKFTKDSKLESGLSFNYRNSLSEDKMYDSIFTTNSFVEDVDKYNRFTYVE
                     NIFSAYTTYSQKIQTFSFQLGLRAEQTMTEAKQHYRSYFSLFPSVFLSQSLPQNNELQ
                     INYTRRINRPRGWQLNDFVNRTDELNLSYGNPSLRPEFTNSFEFNYIKNWEQGHTFST
                     SLYYRATSDVILRVSKLQNEIMETTYENITTSESAGAELILKNRLFANKLDLTSTLNA
                     YYYALAANESYGISSVENFTWNARMNANIIIQRGFTTQLTAYYRAPQVKVQGRDEQVY
                     GIDLGARKTFFKGALNVGVSVRDLLDSQRTTNITWGENFYQESASLYSGRSYRLTLTY
                     NFGNMKNGGKEQRNKNNNGFSEENLEF"
     gene            8820..9590
                     /locus_tag="PLJ82_05515"
     CDS             8820..9590
                     /locus_tag="PLJ82_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR40999.1"
                     /translation="MKKLILLALGFLVATCMGVAQVGVPVVKSTNAPNKDKVNVSTIT
                     FNYPAKDIETAVLEKLKKEGLTPRKSKSKFVVFAGVEYFPLWDNKFDFYLRISGSNNA
                     GTVELLMTQGYNNYINPTTPEVQERVNKWLLGLEKTVSDYIYERAMAEHLKEKKLIER
                     TLSSLQTQQDRLTNQYDNLNKQQEAFEKTKVDVSKADPNTLNTKDIAKQQNQQEKIVK
                     ERNKLEYSLYDVRSRIAASQKDLDNRNKAIDELKQQKR"
     gene            9590..10072
                     /locus_tag="PLJ82_05520"
     CDS             9590..10072
                     /locus_tag="PLJ82_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41000.1"
                     /translation="MARLLLFLIPLLLVSCVAPADYNDSLIREQRRVLSAINDLNTVI
                     KQANKEETNSELAEAVNQVEKSILKVQQKDAFRGETQLREEMEELLVFYKFVLNEYYP
                     LLIEIQHDEGLTLTEKKQVSDSIYDTIREQEIQRTTAYAEAQKLFADKYNISIIHTQK
                     "
BASE COUNT         3243 a         1734 c         1900 g         3270 t
ORIGIN      
        1 ttcgaatttc gaatagtcat aaattccgtt tttgtagaat tcagaagcag cacaatccaa
       61 tccaattgta acatcttttc ctggaacata tcccgcattt tttactgcag taaggattga
      121 ttctagagct tcttcagtac ctccagcaag atttggagca aaacctcctt cgtcgccaac
      181 agcagtactt aatcctttat cgtgtaatac ttttttcaaa gaatggaaaa cttcagcacc
      241 catacgtaag ccttcgcgga aagatttagc acctacaggg cgaatcataa attcttggaa
      301 tgcgatagga gcatcagagt gcgagccccc gttaatgata ttcatcatag gtacaggcaa
      361 cgtaaatgcg tttgtaccgc caatgtaacg gtataatggt aatccaaggt attcagcagc
      421 tgctttagct acagccaaag aaacgcctaa gatagcatta gctcctaatt ttgattttgt
      481 ttttgtacca tctaatgcaa tcattttttt gtcgatagca cgttgttcca tagcacacat
      541 accgataagt tcaggtgcaa ttacgttgtt tacgttagca acagctttta atacaccttt
      601 gcctaagtag cgacctttgt cgccatcgcg taattcaata gcttcgtttt cgccagttga
      661 tgcacctgat ggtacagcag ctctgcctac aattcctgac tctaacgtta catcaacttc
      721 tacagttgga tttccacgcg aatcaagtac ttcgcgggct acaatttttt caattctaca
      781 catgttcttt tttatttata aattgatatt cactaattgt gtttcaaaat ttgacataca
      841 aaagtacaaa aaaaatcttg attattaaat atgtctgtat tattttttgt ttttaatatt
      901 tccccttagc atattcatta caccaatgtt acaaagctat aattcggcga taagttcttt
      961 cattatttgg gtcattttgg gttgcacctg attagcgatg tgttgtactt cttcgtggct
     1021 tacttccaca attttaccag gaacccctaa atctgtaata atggacatag caaaaacctt
     1081 aatgccacta tgaacggcaa caattacttc aggaactgtt gacataccaa cggtgtctgc
     1141 cccaatattt ctaaaataaa cgtattcggc aggagtttca aaagtaggac cagaggtgcc
     1201 gagataaaca ccttctacta ccttaatcgt gtttcttgct gcaatttttt ttgcttttga
     1261 aattaattcc tttgaataag cttgactcat atctggaaaa cgaacgccta attcaggatg
     1321 gtttttacct ctcagtggat gttctgggaa tgtattgata tgatccgtga taatcattaa
     1381 atccccaatt tcaaatgagg gatttactcc tccactggca ttggaaacta ataatgtttc
     1441 aattcctaag gctttcataa cgcgtacagg gaaagtaacc tcttgcatag agtatccttc
     1501 gtagtagtgg aacctacctt gcatcgccag aacttcagtt gttcctaatt taccaataat
     1561 caatttgcca ctatgccctt ctaccgttga aactggaaag tttggaattg tttggtactc
     1621 tatttcttgt ttttctgtaa tttgagaagc taattctccc aatcctgtgc ctaaaataat
     1681 acccacttta ggaaagcgtt gcatttttcc ttttaggtac gttgctgttt cgttgatttt
     1741 tgctaacata atctataatt ttagaattaa atattttttg ttgttgaaat aaaaaattca
     1801 cactgatggg taacatacat gtgtacggtt ttagatggtc aggaaaaagt gggtcgttta
     1861 ccaaacgtgc tgcatctttt tctgttacca ttatcaggcg tttttcctgt ttcatatttt
     1921 caaacatctt tatgatgatt cccatatctt gttgggtaaa aaaatgatgg tcggggaata
     1981 gtagcttctc tacataggga gtcagtgtgc aaatgtaatc tagcagaggt tctggttgtg
     2041 ctatccctgt aactaaaaga attccaaact gttgttcttg aatgatgctt gtagttacta
     2101 tttggttgga tgtaaatacg ctcttgacat caccatatgc aaagcttgta tagaaaatgt
     2161 cttggtacgc taataagttt aaggatttat gtatatttct tgcttcaata ggtgttagat
     2221 tgggtgggca ttttgtgatg agaatgatgt ctgcacgttt aatgccatca atagattctc
     2281 gtaaattacc atatggcaac atattgtctt cagttatagg tctgttataa tcaactaaca
     2341 atattgtttg atttgcctct acataacgat gttgaaaagc atcatcaagt aatacaacgt
     2401 ctgtttgtgg atgagacagt aaaatttgtt taattccatt acgacggtta gcatcaacgg
     2461 ctaccacaat atcaggatgt ttttgatata tttgaaaagg ctcgtctcct attgtttggg
     2521 ctgaagccgt tgcattggca tagatatatc ctaccgtttt gcgtttgtag cctctactta
     2581 gtactgctac attgaatttc ttttttaaga tagatactaa atattcagta tgaggagttt
     2641 taccagtacc acctactgtg atgttaccaa cagaaattac actgattgga aagctttccg
     2701 ttggtaatat gtgtttgtca aacagcatgt tgcgtatact cattgagatg ccgtatagcc
     2761 atgagattgg ccacaatagc atacgtataa aaatgttttt tcgagttacc atatttcact
     2821 attttcttcc atccgagcct gtattattat tctagtttta ttgtcaatga aggaacgtat
     2881 taacctatac tcatgcagag gaatgttttt agtaagcaaa gattgaatct gtatcgttgt
     2941 accttctaac atgctttccc atactgtttt ctttttaggg aggtgttcta cctcatagct
     3001 tgtaattgat gccatacgag cagccgattg tatggcttgt tgcagccctc ctattttgtc
     3061 tactaagccg tgtgttaaag cgtccgatcc tgtccatact ctgcctccag ctagtggtag
     3121 taatgctgct tttgtgatag atcgaccctc agaacatctg tgtaaaaaca actgataatt
     3181 atctgtaata tatctttcca agatgttacg ttcatatgtg gtcatgtctc ttgtaaaaga
     3241 aggaaagtcc gctaatgtat gggttgtttg agggtcgtaa gtaatcccaa atttctcaga
     3301 aatttttgag aagtttggga taataccaaa aataccaatg gatccagtaa gcgtttcggt
     3361 attactgaaa atagaatcag cttgagttgc aatataatag cctcctgaag cagcataatc
     3421 gcccattgat acgactaagg gtttcacccc tttaaaaaga gatgtttctc tccaaatgcg
     3481 ttctgctcca aaagcactgc ctcctggcga attaatgcga agtactactg cttttataga
     3541 agaatccttt cgcgtttctg atagcaattt agacagttct tcagaatcaa ttccttcaat
     3601 atcgccatca tcaattcccc cattagcata tagtacggca attttattgt tatttgattt
     3661 gttttgtgat gcaagaacta tgtagtctcc atagctaacc atttgttttt cttcgttata
     3721 cgtgctatca atgtgggttt tgaaatagga aacaagttcg tctgggtata gtaagctatc
     3781 tacaagccct tctgtaacgt acgactttgt tggggcaaac attaccgatt tttcaaccaa
     3841 ggattctatt tgttcaatgc ttatatttct ttcggatgca atatttgtac acatttttcg
     3901 ccagagttgg ttgataatgt tttttgtttg agctttattc tcatcactca tagcattatt
     3961 tgtatacatt tcaggtgctc ctttataatc gcccactttt acaacctgca tatcaacgcc
     4021 tactttttgg agtgcatttt taaagaaaat aggttttgaa gccaatcctt tgagttcaat
     4081 tgttcccgat ggatgtataa aaatcttatc agcaaccgtt gctagataat ataattgttg
     4141 ggtgtaatta tccccataag caataataaa tttacctgat tttttaaaat cgactaatgc
     4201 atctcgaatt tcgcttatag aggcatttcc agcactaaat aaatccattt gcaaccaaat
     4261 tccaacaata ttttcgtctg ttttggcatg ctgaatgctc tttattatat cgtgaatacc
     4321 aagttcggtt atatttgtgg atgaccacgc atcaaaaaag gacatatctc ttgcttgctc
     4381 tttcaaaacg ccgttaaatt caatttttaa tacagaatta ggtgcaaccg ttatttgttt
     4441 agatgaaaat ccagtaacag tagcaacaat aactattgca actataatgc atattgtaaa
     4501 aaaagaaagt aagaagccca agcaagaagc aaaagtaaat ttcagaaatt gtttcatctt
     4561 gaatctaaaa tatgtacaaa attatacgta aagtaaatca taaactacaa agatagcgat
     4621 ttaaagatta aaataaaata gttttgtgtc aaattaaaat gttgtaaaaa aaacttctat
     4681 aaaaaccaaa catacaatac ttgtatttgc taaaaaaaca ttacttttgc acttatatat
     4741 ttatcacaca tagctataaa catttttaga atttcgcaga tggaagaaat taactggtcg
     4801 aatttgtcgt ttagttatat gccaacggat tgtaatattc ggtgttcata tcaaaacgga
     4861 gagtggggag aattacgaaa acatacagat gtgtatttga atatgcacat ggctgctact
     4921 tgtttgcatt acgggcaaga atgtttcgaa ggactgaaag cttttagagg aaaagatggt
     4981 aagattcgtg tgtttaggat tgatgaaaat gcacttcgta tgcaatcgtc gtgtgaaggt
     5041 atactgatgg caaaatttcc agttgacaaa ttcaaagagg cggttaaatt ggttgtaaaa
     5101 gaaaatgaac gctttgtgcc accttacgaa agtggagcct cgttatacat acgtccgtta
     5161 attataggta ccagtgctca ggttggtgtg aatgcagcaa aggaatatga gtttattttg
     5221 tttgtaacgc ctgttggtcc gtattttaag ggcggttttc aaactactcc gtttgtgata
     5281 ttacggcagt ttgatcgtac agctccttta ggaacaggta tgtacaaagt gggtggtaat
     5341 tatgcagcaa gcttggttgc tgtagataaa gctcataaga tggggtattc aaatgtattt
     5401 tatttagatg caaaagaaaa aaaatacatt gatgaatgtg gtgcagcaaa cttttttggg
     5461 attaaaaaca atacgtacat aacaccaaaa tcaacgtcaa ttttaccatc aataaccaac
     5521 aaaagtttaa tggttttggc acaagaattg ggtttaaagg tagagcaacg tccagtccct
     5581 gaagaagaat tggctacttt tgaagaagct ggagcatgcg gaactgcagc tgtaataagt
     5641 cctattcagc gaattgatga tgtagaggaa ggaaagtcat atgtgttttc tgcagatggt
     5701 aaaccaggga aagtatgcga acaattatat actaaattac gtgctattca atacggagat
     5761 gagccagata aatatggttg gataacagtt attgagtaaa aaaacgaaca accaactgtc
     5821 tttacattga aacttacgct atgcaattag ttattataaa tggtcctaat ttaaatttgc
     5881 ttggcaaaag agagccgcaa gtttatggaa atcaaacatt tgaggaaatg cttgcggaaa
     5941 tccgaaaagc atttccatca attgatattc tgtattttca atctaacgta gaaggcgaaa
     6001 ttattaataa aattcatgaa gtagggttta ctgctgatgg tattattatc aatgcaggag
     6061 cctatacaca tacttctatt gctattgccg atgctgtaaa atccattaaa actcctgtcg
     6121 tagaagtaca tatatctaat gtttttgctc gcgaatcata ccgacacgag tcttttttaa
     6181 ctcccgtttg caaaggctgt atttctggat ttggaattaa atcctattat ttagccatac
     6241 agagtttttt ttgacataaa ataacatttc cgctttaaac ctttttctct tttttccgtc
     6301 atagaggcac aacacctaaa cgtaacgaat atgaaatata aatatgtgtt aagtctacta
     6361 tggctttttt ttgtcgtgtt tattatacgg gctcaagacg gaaaaattat tggtcaagta
     6421 tttgataata cgtctaaaaa accgcttgat tttgccaatg tagctctatt tactaatcaa
     6481 tcaaaagagc cattacaagc aatattgtct gatagtgaag gtaagtttat tcttcagaat
     6541 ttaccctatg ggtcgtttac attgaaagtt tcttttgttg gctatgaatc ttttgaaacc
     6601 tctgtgttgg tgagcaaaga gaaacctaca gctagggtaa ataaagttgt attgaaagaa
     6661 aatgcacaac aaatatcaga ggtgcaggta gttggtcagc gttcgggagt atcttttgaa
     6721 atagataaaa aagtatttaa tgtggatgaa actgcacttg cacaaggttc ttccacaact
     6781 gatgtgctaa aaaatattcc ttcggtagcg gttgatttag aaggaaatgt ttcgttgcga
     6841 aataattcgt cagtagaaat ttggataaat ggcaagccat ctggtttgac agatgaaaat
     6901 agagggcagg tgctagagca aatgccagcc gaaacggtag aaaagataga ggttataacc
     6961 aatccatcag cccgttttaa tccagaggga agtgcaggaa ttattaatat tgtattgaaa
     7021 gaaaaaaatc gtggctccta tttagctagt attaatagca atattggtta taatgagtcg
     7081 ggtgctataa atggttcttt tggtggtaat tatatgtaca acggtcccaa atttgatgtg
     7141 caattgaatg gttctatacg aggaggggaa cgtaaaaatg catcatacaa caatcgggca
     7201 actttggtag cagaagatac tatctcgcat cttaatcaac gatcgttatc tgagtcagat
     7261 atgttcatgg gctttatgag aggaggcgta acctatcata tcgacaaaaa aatgatattg
     7321 gacttagtgg ttttggtatg ttaggagatt tttcggggaa tagcacaatt aattatcatt
     7381 cattagatgc aaatagagat acccttgaaa cgagggacag attagctgat aatgcaaata
     7441 atcggatgca ttacaatgta tctcttgatt acaagcattt ttttgataaa actaaccacg
     7501 agttgtcgtc gtctatttat tatgtgcgag gtaatcataa tagctttaat gcctacaata
     7561 cgtcccgttt tggtaacgat ggagtgtata tcaacgattc aaaaacatat caagagcaac
     7621 aagctacacg caatatgtat aacattactt ggcaggctga ttactttaat aaatttacca
     7681 aagactctaa actcgaaagt ggcttaagct ttaattatcg caattcgttg agcgaagata
     7741 aaatgtatga ttccatcttt actaccaatt cttttgtcga agatgtagac aagtacaatc
     7801 gatttactta tgtggaaaat attttttcag catataccac ttactctcaa aaaatacaaa
     7861 catttagttt tcaacttggc ttgcgtgctg aacaaacaat gactgaggca aagcagcatt
     7921 ataggtctta ttttagttta tttccttcgg tctttctatc acaatctttg ccacagaaca
     7981 acgaattgca gataaattat acgcgacgta ttaatcgtcc acgaggttgg caactcaatg
     8041 attttgttaa ccgtactgac gaattaaatc tgtcatacgg aaatcctagt ttgcgtcccg
     8101 agtttactaa ttcatttgaa tttaattata taaagaactg ggagcaaggt catacgtttt
     8161 ctacctcatt gtattatcgt gctacttctg atgttattct gcgtgtaagc aagttgcaaa
     8221 acgaaattat ggaaactact tacgaaaata ttacaacatc agaatcagca ggagccgagt
     8281 tgattttaaa aaacaggtta tttgctaata aattagattt aacctctaca ctcaatgcct
     8341 actactatgc gttagctgcg aatgaaagtt atggcatttc atcggtagaa aattttacat
     8401 ggaatgcaag aatgaatgcg aatattatta ttcaacgagg ttttactaca cagctaacag
     8461 cgtattatcg tgctccacaa gtaaaagtgc agggtcgtga tgagcaagtg tatggtattg
     8521 atttaggagc tagaaaaaca ttctttaaag gggctctcaa tgtgggagtg tctgtcagag
     8581 atttgcttga ttctcaacga actaccaaca ttacgtgggg cgaaaacttt tatcaagaaa
     8641 gtgcaagtct ctattcagga agaagttata ggctcaccct tacgtataat tttggcaaca
     8701 tgaaaaatgg aggcaaagaa cagcgtaaca aaaacaacaa tggttttagt gaagaaaacc
     8761 ttgaatttta aaaaatattg ctttatcttt gtgtactatt aattgaaaaa aactaaggta
     8821 tgaaaaagct tattttacta gcattgggtt ttttagtagc tacttgcatg ggagtagcac
     8881 aggttggtgt gccggttgtt aaatcaacaa atgctccgaa taaagataaa gtaaacgttt
     8941 caactattac attcaattat ccagctaaag atattgaaac tgcggtatta gaaaaactaa
     9001 aaaaagaagg tctaacacca cgaaaatcta aatcgaaatt tgtcgttttt gctggagtag
     9061 aatacttccc cctttgggat aataaattcg atttttatct tcgtatttca ggctctaaca
     9121 atgcaggtac cgttgaatta ttaatgacac aagggtataa taattatatc aatcctacta
     9181 ccccagaagt acaagaaagg gtgaataaat ggttgcttgg attggaaaaa accgtgagtg
     9241 attatattta tgagcgagct atggcagagc atttgaaaga gaaaaagttg attgagcgta
     9301 ccttatcctc tttacaaacg caacaagata gattaacaaa tcaatacgat aatttaaata
     9361 aacaacaaga agcgtttgaa aaaaccaaag tagatgtctc taaagcagat ccaaatacct
     9421 tgaataccaa agatatagcc aaacagcaaa accagcaaga gaaaattgtg aaagaacgta
     9481 ataagctaga atatagcttg tatgacgtac gttcgagaat tgcagcttcg caaaaagatt
     9541 tagacaatag aaacaaagca attgacgaac tgaaacaaca gaaacgctaa tggcaagatt
     9601 gctattattt cttattccgt tgttgttggt gtcgtgtgta gcacctgctg attataacga
     9661 ttcgttgata cgcgaacagc gtagggtgct gtctgctatt aatgacttga atacagttat
     9721 caaacaagcc aacaaagaag aaaccaatag tgaactagca gaggctgtca atcaggtaga
     9781 aaaatctatc cttaaagtgc aacagaaaga tgcttttaga ggggaaacac agttgcgaga
     9841 ggaaatggag gagttgcttg ttttttataa gtttgtttta aacgagtatt atccattgct
     9901 gatagaaatt caacacgatg aaggcttaac gctaactgaa aaaaagcaag tttccgactc
     9961 tatttacgat accatacgtg aacaagaaat tcaacgtaca actgcttatg cagaggctca
    10021 aaaactgttt gccgacaagt acaatatatc catcattcat actcagaaat agaaactacc
    10081 atggaggaaa ccttgttgca actttttttg cgtgtaatag gcgaacagcc tacatccgtt
    10141 gtagcat
//
LOCUS       DAOSRP010000069        10141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6435_length_10141_cov_5.003668, whole genome shotgun sequence.
ACCESSION   DAOSRP010000069 DAOSRP010000000
VERSION     DAOSRP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_10141_cov_5.003668"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1412
                     /locus_tag="PLJ82_05525"
     CDS             282..1412
                     /locus_tag="PLJ82_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41001.1"
                     /translation="MVIILFSSCEEWNIKTYNVPSEFQPYVDKFKTDAKKYGYNFDDK
                     GLIVRFADLDNNIAGLAYYKRNPILIEIDREYWASASNTKNAHDIKENLLFHELGHGF
                     LLRMHDNTVLANGDWKTIMCGDKLPNDRASNINYRGFRKAYYIEELFTRTNDTPAWST
                     LIPQFDNIDENVILQQDFSSGSDWTIGSNSLYESSIENGAYTFTTKTSQAFYVLNKGT
                     LNTSNDFYVEVRLKASAGLDDSFGLVCGSFNDGNTPTSLHYFYQKGNNHMYIGESECL
                     GPFIDLYTEILHPNEFNTFAIRKYNNMLYYYINDTFIYHNDLDEIITMYGSQIGFKIP
                     GNSTLYVDYAEVRENSTGLKKRNTTELSVEKATEKKVIHWNK"
     gene            complement(1417..2016)
                     /locus_tag="PLJ82_05530"
     CDS             complement(1417..2016)
                     /locus_tag="PLJ82_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR41002.1"
                     /translation="MSRTRVIHTTITSTRELLLEVARQLFAKVGFTNTTMNDIAKAAK
                     RGRRTLYVYFKSKEDVYYAVVEQEMYKLLDALELVANSAEPPQNKLIDYIYTRLDKTR
                     EVVKNNGTLRADFFSDYKTVEMVRKKMEIKEYELLKKILKEGVDAGIFYVRSIDFAAF
                     LVLNAIRGLELPYMKQSVADRINDSREDIVRVLFHGLLK"
     gene            2141..3469
                     /gene="tilS"
                     /locus_tag="PLJ82_05535"
     CDS             2141..3469
                     /gene="tilS"
                     /locus_tag="PLJ82_05535"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOR41003.1"
                     /translation="MIHLVTHYIHTHKLLSPAATVIVGVSGGADSMALLYILKEIGYN
                     CVVAHCNFTLRQKESDADESFVVDYCRTNNLPCLVKHFDTKKHAQDNKISIEMAAREL
                     RYGWFETLRLHYQAEAIAIAHHFNDSIETILINLINGTGINGLTGIKPINGHIIRPLL
                     CVNRKEIEDFLQKKHLHFITDSSNVENIYTRNKIRNILIPQIEEINPSFINTMQGTIH
                     NLTTSATIYNSYIELKLKDIIESSPNNQVKISINQLLKEEHADTVLYEALKTYNFSPK
                     LCASILNNVANSGKEYLSPTHKLLIDRDFIFLTPIKEKNDTHTYYIQQSDSSIEFPIK
                     LNINILRNDASFHLSKKSTCACIDYSLLKFPLTIRRWKKGERFYPLGMKQSKKVSDFF
                     VDNKIPTWEKEKCWIIESNGNIVWIIGYRLDDRYKVTTSTSLIFKISVKE"
     gene            complement(3475..4377)
                     /locus_tag="PLJ82_05540"
     CDS             complement(3475..4377)
                     /locus_tag="PLJ82_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOR41004.1"
                     /translation="MSHHHHHHHSPIVESSFSLGKAFFFAIVLNVLFVVAEFATGFIY
                     NSIGLISDAAHNLGDVASLLLAWLAFKLAKVSTKTNYTYGFKKSTILVALTNAIILLF
                     TVGVIIVKSISKFYYPSPVEGGVIAWVAGVGVVINLATAFLFIRDKEKDLNVKGAYLH
                     MMADAIVSVGVVVSGITILFTGWNFLDPIIGLLIAVVILFSTWNLLRDSIRLSLDGVP
                     SSVDIEEIKSVILQHPAVESIHHLHIWAISTTETALTVHVVLRNLETMEISKQELKKI
                     LAAHLISHATIEIETADGMCGELC"
     gene            complement(4401..4577)
                     /locus_tag="PLJ82_05545"
     CDS             complement(4401..4577)
                     /locus_tag="PLJ82_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOR41005.1"
                     /translation="MDSVAKVLDVIKKASEPVNATKVVELSGLDKKEVDKAMKTLKDA
                     GHIVSPKRCYWSAK"
     gene            complement(4725..8267)
                     /gene="nifJ"
                     /locus_tag="PLJ82_05550"
     CDS             complement(4725..8267)
                     /gene="nifJ"
                     /locus_tag="PLJ82_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOR41006.1"
                     /translation="MAKQKKFLTCDGNQAASHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAANGRKNIFGEPVQVQEMQSEAGAAAAMHGALQAGALTTTFTASQGLLLMIPNMYKV
                     AGELLPGVYHVSARALASHALSIFGDHQDVMAVRQTGCALLATGSVQEVMDLAGVAHL
                     AAIKTRVPFVHFFDGFRTSHEIQKVEQLDQEDLIPLIDQEALQAFRKRALNPESPVLR
                     GTAQNPDIYFQAREACNPFYDAIPAVVEDYMAQLSAITGRNYGLFDYYGSQDAERIIV
                     AMGSATEAIREGIDYLVAKGEKVGLISVHLYRPFSAKHFLSVMPSTVKRIAVLDRTKE
                     PGAGGEPLYLDVKDVLYGQKNQPLVVGGRYGLSSKDFTPSQVFAIYENLALPEPKNHF
                     TVGIVDDVTFTSLPMKEEFSLADAGTYEAKFYGLGADGTVGANKNSIKIIGDNTNKYC
                     QAYFAYDSKKSGGFTCSHLRFGDTPIRSTYLVNTPDFVACHVQAYLKLYDVTKGLKKN
                     GTFLLNTVWTPEEVKKNLPDNVKKYLAKNNITLYIIDATNIALELGLGNRTNTILQSA
                     FFKITNVIPYELAVEKLKYAINKSYGKKGEKIVNMNYAAVDKGGEYIKVDVPAEWANI
                     ALESDLSTTTVPEFIQKVVRPINAQDGDSLPVSSFTGREDGTWNQGTAKYEKRGVAAS
                     VPVWDAENCIQCNQCAYVCPHAAIRPFVLDEAEQAKAPFKEMLKANGKQFAGMAFRIQ
                     VDVLDCMGCNNCVDVCPGNKGNKALELVAIETQYPNQDNWDFCMNEVSSKQHLVDVKA
                     NVKNSQLATPLFESSGACAGCGETPYVKLVTQLYGERQMVANATGCSSIYSASAPSTP
                     YTTTPEGRGPAWANSLFEDNAEFGLGMVFAEEHMRARIVRIMNEAIASDCCPNEIKDL
                     FKEWLENRLDEQKSKELQKQITPLVKASTCSHCQQIATMTQYLVKQSQWIIGGDGWAY
                     DIGFGGLDHVIASGKNVNILVLDTEIYSNTGGQASKSTPVGAVAKFAASGKKVRKKDL
                     GLIAATYGYVYVAQVAMGANQAQTLKAIREAEAYDGPSLVIAYSPCISHGIAAGMGKA
                     QEEQKRAVECGYWHLYRYNPDMELEGKNPMQLDSKEPDWSKFQDFLKGEVRYTSLLKQ
                     FPQEAKELFEAAENNAKWRYNSYKRMANQQWNVE"
     gene            complement(8333..10051)
                     /gene="rho"
                     /locus_tag="PLJ82_05555"
     CDS             complement(8333..10051)
                     /gene="rho"
                     /locus_tag="PLJ82_05555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOR41007.1"
                     /translation="MSTKNELEKKTLSELQRIAKELSVPKISSLEQQALVYAILDKEA
                     EKVAQKTVTKPTTVVARKEKRARVTKPVGKSNEKADDKSIETPVSVVELIPDVHKEDA
                     LKQQIVEVPKKKVITKKPTPPTLLKKTVESIQIDNQQAEEKVSVISSISTVNTVVVDE
                     KQEENTGVDLPVTTQPNVVIKKEQRVITKPNHSNENSEKLYEFDNILKGTGVLEMVEN
                     YGFLRSAEYNYMPSPDDVYVSQSQIRLFGLKTGDFVDGYIRPPKEGEKYFPLVKVEKI
                     NGRDPAFVRDRVPFEHLTPLFPEEKFILNGRNSTLSTRIIDLFCPIGKGQRGLIVAQP
                     KTGKTVLLKEIANAILEHNPEVYMIILLIDERPEEVTDMARSVKAEVVSSTFDESAEK
                     HVKVASIVHEKAKRLVECGHDVVILLDSITRLARAYNTTAPASGKVLSGGVDANALHK
                     PKRFFGAARNIENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRRLSNKRI
                     FPAVDIMASSTRRDDLLLGTDILQRMWILRRHLSDMNSVEAMEFLKDRMEKTRDNEEF
                     LASMNG"
BASE COUNT         3278 a         1975 c         1484 g         3404 t
ORIGIN      
        1 cactaaggta aaattaggtg ctactgaacc tactttaggt aaatctccat tggtatttac
       61 ctctccgcct ttaaaaagta tttttgccat ataatatagt tatttaaatt aaacaaacgc
      121 aatgtacgtc ttttttctaa tagaaattct aaaatcaaag tattttttgc ttttttttaa
      181 taaaaaagag aatatttgac agttaataca taaaaaaaga acttacattt gtaaattact
      241 ttaagctaaa aaaaataatg cgtaatgctt tctttttcgt tttggtcata atactctttt
      301 cttcatgtga agaatggaat ataaaaacct ataacgttcc gtctgaattt caaccttatg
      361 tagataagtt taaaacagac gccaaaaaat atggatataa ttttgatgac aaaggactaa
      421 ttgtacgttt tgctgattta gacaacaata ttgcaggctt agcttattac aaaagaaatc
      481 ctattttaat tgaaattgat agagaatact gggcatcggc tagtaataca aagaatgcac
      541 atgatattaa agaaaaccta ctgtttcatg aattagggca tggatttttg ctacgtatgc
      601 acgataatac tgtattagca aatggagatt ggaaaacaat tatgtgtggt gataaactcc
      661 caaacgacag agcctctaac atcaattata gaggttttag aaaagcatac tatatcgaag
      721 aactatttac tcgcaccaac gatacgccag catggagtac actcatccct caatttgata
      781 atatcgacga aaacgtcatt ctacaacaag atttttctag tggttcagat tggacaatag
      841 gctccaatag tttatacgaa agttccattg aaaatggagc atatacattt accaccaaaa
      901 cttctcaggc tttttatgta ttaaacaaag gtactctgaa tacaagcaat gatttttacg
      961 tagaggttcg actcaaagca tcagcaggtc ttgatgactc atttggactt gtttgtggct
     1021 catttaacga tgggaatacg ccaacttctc ttcattattt ttaccaaaaa ggaaataatc
     1081 atatgtatat aggagagtcc gaatgtttag gtccatttat tgatttatat acagaaattt
     1141 tacatcctaa cgaattcaac acatttgcta tacgtaaata caataatatg ttgtactatt
     1201 atatcaacga cacctttatc tatcacaacg atttagatga gattattacc atgtatggct
     1261 cacaaattgg gtttaaaatt cctggcaata gcaccctata tgttgattat gcagaggtca
     1321 gagaaaatag cactgggttg aaaaaacgaa ataccaccga acttagtgtg gaaaaagcaa
     1381 cggagaaaaa agtcattcat tggaataagt aacaggctac ttcaataacc catgaaacaa
     1441 cacacgaacg atatcttccc tgctatcgtt tattctatca gctacagact gtttcatata
     1501 gggtagctct aatcctctaa ttgcattcaa tactaaaaaa gcagcaaaat caatacttct
     1561 aacataaaag attccagcat caacaccttc tttaagaatt ttctttagta attcatattc
     1621 tttgatttcc atttttttac gtaccatttc caccgtttta taatcactaa aaaaatcagc
     1681 tctcagtgta ccgttatttt taacaacctc tctggtttta tctaatcgtg tatagatata
     1741 atcaatcaat ttattttgag gaggttcggc agaatttgct actaactcca aagcatccag
     1801 caatttatac atttcctgtt ctactactgc ataataaaca tcttccttgc ttttaaaata
     1861 aacgtataag gttcgcctgc cccgtttagc cgctttagca atatcattca tagtagtatt
     1921 cgtaaaacca actttagcaa acaattgcct ggcaacctct aacaataact ccctagttga
     1981 cgtaatggtt gtatgtataa ctctagttct actcattgca caataatgtt actttgtgca
     2041 aaaataaggg agttatttca atttatcaaa taattaagca ttttttttat tattcctcca
     2101 aaaacgtaaa aaatatatcg tacttttgca tatatagcat atgatacatc tagtaacaca
     2161 ctacatacat acacacaaat tactctctcc tgcagctact gttatagttg gggtaagtgg
     2221 aggagccgat tctatggcat tactctatat tctcaaagaa atcggataca attgtgtggt
     2281 ggctcattgt aattttacat tacgccaaaa ggagtcggat gcagatgaaa gttttgttgt
     2341 tgattattgt cgcaccaata atctaccctg tttagtcaaa cactttgaca caaaaaaaca
     2401 tgctcaagac aataaaattt ctattgaaat ggcagcgaga gaacttcgct acggctggtt
     2461 tgaaactctt cgattacact atcaagccga agccattgca atagctcatc acttcaatga
     2521 ttctattgaa accattctca taaacttaat caatggaaca ggtataaatg gattaacagg
     2581 tatcaagccc ataaatggac atattattcg tcctttatta tgcgttaata gaaaagagat
     2641 tgaagatttt ttacagaaaa aacacttgca ttttattact gactcaagta atgtcgaaaa
     2701 tatatatact cgcaataaaa taagaaatat tcttattccc caaatagaag agataaaccc
     2761 ttcttttata aacacaatgc agggcactat ccacaattta acaacatctg ctactattta
     2821 taatagctac attgagttga agctaaaaga tattattgaa tcaagcccaa ataatcaagt
     2881 taaaatctct attaatcagt tactaaaaga agaacatgca gacactgtgc tatatgaagc
     2941 tctcaaaacg tataattttt cacctaaact atgtgcatca atactcaata atgtagcaaa
     3001 tagcggaaaa gagtacctgt caccaacaca taaactcctc atagacagag attttatttt
     3061 tttaactccc attaaagaaa agaatgatac acatacctat tatatccaac agtctgattc
     3121 atcaatagaa tttcctatca aactgaatat aaacatctta cgaaacgatg cgagttttca
     3181 tttatccaaa aagtcaacct gtgcttgcat tgattattct ttgctaaaat ttcctcttac
     3241 aattaggaga tggaaaaaag gggagcgatt ttatccttta ggaatgaaac aaagtaaaaa
     3301 agtaagcgat ttttttgttg acaacaaaat acctacatgg gaaaaagaaa aatgttggat
     3361 aatcgaatca aacggaaata ttgtatggat aattgggtat agacttgatg acagatacaa
     3421 ggtaactacc tcaacctcac ttatatttaa aatatctgta aaagaataaa gggtttaaca
     3481 taattctcca cacataccat ctgcagtttc tatttctatt gttgcatgac taattaaatg
     3541 tgctgccaag attttcttta actcttgctt actaatttcc attgtttcta aattacgtaa
     3601 cacaacatgc acagtcaaag cagtttcagt tgtacttatt gcccaaatat gcaaatgatg
     3661 aatactttcc acagctggat gttgaagaat caccgattta atttcttcta tatctacgct
     3721 agacggaact ccatctaaag ataagcggat gctatcacgc aataaattcc acgttgaaaa
     3781 taaaataaca acagctatca gcaaacctat tataggatca agaaaattcc atccagtgaa
     3841 tagaatggtg atccccgaaa cgacaacccc aacagataca atagcatccg ccatcatatg
     3901 caaataagct ccctttacat tcaaatcttt ttccttatcg cgaataaaaa gaaaagctgt
     3961 tgctaaatta ataacaaccc ctactccagc aacccaggca atcacacctc cttctacagg
     4021 acttggataa taaaattttg aaatgctttt aacaataata actcccaccg taaacaacaa
     4081 aattatagca ttcgtaagtg ccacgagtat ggtgcttttt ttaaaaccat atgtatagtt
     4141 tgtttttgtt gacacctttg ccaatttaaa tgccaaccaa gctaacaaca aactagccac
     4201 atctcccaaa ttatgtgctg catctgatat taatcctatt gaattataaa taaaaccagt
     4261 ggcaaattca gcaactacaa ataatacatt cagcacaatg gcaaaaaaga aagctttccc
     4321 taacgaaaaa gaagactcta ctattggtga atgatgatgg tggtggtggt gagacatact
     4381 attgtaatgt tatgattgag ctatttagca ctccaataac aacgctttgg agacacgata
     4441 tgcccagcat cttttaatgt cttcattgcc ttgtctactt ctttcttatc taaaccagat
     4501 aattctacta cttttgttgc atttacaggt tctgatgctt tttttatcac atctaatact
     4561 tttgctactg aatccatatt ctatctattt ttaaattaat aaataatcaa aattacaaaa
     4621 atatatgtag tagctatgtt tatttttaga tattttaata aaaaaggaga gtatatgttt
     4681 tttactccat atactctcct tgcattttac aatcattttg ctagttactc aacattccat
     4741 tgttgatttg ccatacgttt atatgaatta taacgccatt tagcattatt ttcagcagcc
     4801 tcaaatagtt cttttgcttc ctgagggaat tgtttcaaca atgatgtgta acgaacctct
     4861 ccttttaaga aatcttggaa tttgctccaa tctggttctt tagaatctaa ttgcattggg
     4921 tttttgccct caagttccat atctggatta tatcgatata agtgccagta accacattca
     4981 acagcacgtt tttgctcttc ttgagctttg cccatacctg cagcaatacc gtgactaata
     5041 catggagaat acgcaattac aagagatggt ccatcatatg cctctgcctc tctaatagct
     5101 ttcaaggttt gtgcctgatt agctcccatg gctacttgtg ctacatacac ataaccataa
     5161 gtggctgcta ttaaacctaa atctttttta cgtactttct tacctgatgc agcaaattta
     5221 gctaccgctc caactggagt tgatttagat gcttgtccac cagtattaga gtaaatttcg
     5281 gtatcaagca ccaaaatgtt aacatttttt cctgatgcaa tcacgtgatc taatccacca
     5341 aaaccaatat cgtaagccca accatcacca cctataatcc attgcgattg tttaactaaa
     5401 tactgtgtca ttgtagcaat ttgttgacaa tgagaacatg tacttgcttt aactaatgga
     5461 gttatttgtt tttgtaattc tttagacttt tgttcatcta atctattttc aagccattct
     5521 ttaaataaat ctttaatttc atttggacaa caatcgctag ctatcgcttc attcataata
     5581 cgtacgatac gtgcacgcat atgttcttct gcaaacacca tacctaaacc aaattcagca
     5641 ttatcttcaa acaatgaatt tgcccatgct ggacctcttc cttctggtgt tgttgtataa
     5701 ggagtagaag gtgctgatgc agaataaatg gagctacaac ctgtagcatt tgccaccatt
     5761 tgtctttctc catacaattg tgttaccaat tttacataag gagtttcgcc acaaccagca
     5821 caagctccag atgattcaaa taaaggagtt gctaattgtg aattctttac gttggctttt
     5881 acatccacca aatgttgttt cgaacttact tcgttcatac agaaatccca attgtcctga
     5941 tttggatatt gtgtttcaat cgctaccaat tcaagagcct tgtttccttt atttcctggg
     6001 cacacatcca cacagttatt acatcccata cagtctaata catcaacttg tatacgaaat
     6061 gccatacctg caaattgctt accatttgct ttaagcatct ctttaaatgg ggctttagct
     6121 tgctctgctt catctaacac aaacggacga atagcggcat gaggacacac ataagcacat
     6181 tgattacatt gaatacaatt ttccgcatcc catacaggta cagaggctgc cacaccacgt
     6241 ttttcgtatt ttgctgtacc ttgattccat gtaccatctt ctcttcctgt aaatgacgaa
     6301 acgggaagac tatctccatc ttgtgcatta attggacgaa caactttttg aataaattct
     6361 ggcacagtag tagtagacaa atcactttcc aaagcaatat ttgcccactc agcaggaaca
     6421 tctactttta tatattcacc acctttatcc acggcagcat aattcatatt tacaattttt
     6481 tctccttttt ttccatatga cttgttaata gcatatttta atttttctac cgctaattca
     6541 taaggaatca cattggtaat tttaaagaaa gccgattgta aaatagtatt agtcctattg
     6601 cctaaaccaa gttctaaagc aatatttgta gcatcaataa tatacaaagt aatgttattt
     6661 ttagctaaat atttttttac attatcaggt aaattctttt taacctcttc tggtgtccaa
     6721 actgtattca acaaaaatgt cccattcttt ttcaaccctt tggttacatc atacaatttt
     6781 aaataggctt gcacatgaca tgctacaaag tctggtgtat ttactaaata agtagaacgg
     6841 ataggtgtat ctccaaaacg aagatgagaa caagtaaaac ctcctgattt tttagagtcg
     6901 tatgcaaaat atgcttgaca atatttattt gtattatctc caataatttt gatagaattt
     6961 ttatttgcac ctaccgtacc gtctgctcct aatccataaa atttagcttc gtaagtacca
     7021 gcgtctgcca atgaaaattc ttctttcatt ggtaaagatg taaaggttac atcatctaca
     7081 atacctactg taaagtgatt cttaggttct ggcaatgcca aattttcata aatagcaaat
     7141 acttgtgatg gagtaaaatc tttagacgac aatccgtatc tacctcctac aacaagaggt
     7201 tgattttttt gtccatacaa tacatctttc acatctaaat acaacggttc tcctccagct
     7261 ccaggttctt ttgttctgtc aagtacagca attcttttta ctgtgcttgg cattaccgat
     7321 agaaaatgtt tagcagagaa tggacgatat aaatgcactg aaataagacc taccttctct
     7381 ccttttgcta ctaaataatc aattccttcg cgaatagcct ctgttgctga acccattgct
     7441 acaataattc tctcagcatc ttgtgaacca taataatcaa ataatccata attacgtcca
     7501 gttattgctg aaagttgtgc catataatct tctacaacag caggaattgc atcataaaaa
     7561 ggattacaag cctcacgtgc ttgaaaataa atatctggat tttgagcagt accacgtaat
     7621 actggacttt ctggatttaa cgctctttta cggaatgctt gtaatgcttc ttgatcaatt
     7681 aaaggaatta aatcttcttg gtctaattgt tctacttttt gaatttcatg tgatgttcta
     7741 aatccatcaa aaaaatggac aaaaggaaca cgtgttttta ttgctgccaa atgagctact
     7801 ccagccaaat ccataacctc ttgaacagat ccagttgcaa gcaaagcaca tcctgtttga
     7861 cgaacagcca taacatcttg atggtctcca aaaattgata aagcatgtga tgctagtgca
     7921 cgagcagaca cgtgatatac tcctggcaat aattcaccag caactttata catattagga
     7981 atcatcaaca ataacccttg tgatgccgta aatgtagtgg ttaaagcccc tgcttgtaat
     8041 gccccgtgca tagctgctgc tgctccagct tctgattgca tttcttgtac ttgtacaggt
     8101 tctccaaaaa tatttttgcg tccatttgct gcccactcat ctacatattc tgccatagtt
     8161 gaggatggcg tgattgggta aatagcagct acttcactaa acatatatgc tatatgcgaa
     8221 gctgcttgat taccatcaca tgtgagaaat tttttttgtt tcgccatttt cgtaatgatt
     8281 aattattaat taagttgtgt atttttttat tcagactgac aaagatataa aattatccat
     8341 tcattgatgc aagaaattct tcattatctc gtgttttttc cattctatct ttcaaaaatt
     8401 ccattgcctc taccgaattc atatcggata aatgtctacg caatatccac attcgttgca
     8461 aaatatctgt tcccaacaac aagtcatccc tcctagtact tgatgccatt atatctactg
     8521 caggaaatat tcttttattt gataaacgcc tatctaactg taactccata ttacctgtgc
     8581 ctttaaattc ctcaaaaatt acatcatcca ttttagaacc tgtttcagtt aaagcagtag
     8641 caataatggt taaacttcct ccattttcaa tatttcgagc tgcaccaaaa aaacgtttgg
     8701 gcttatgcaa agcatttgca tctacacctc ctgataatac tttaccagat gctggtgctg
     8761 tagtattata cgctcgagcc aaacgggtaa tcgaatctaa caaaataaca acatcatgac
     8821 cacactcaac aagtctttta gctttctcat gcacaatact cgccacttta acatgctttt
     8881 cagcagactc atcaaaggta gatgatacaa cctctgcttt tacacttcta gccatatctg
     8941 ttacttcttc tggacgctcg tcgatgagca aaataatcat atacacttct ggattatgct
     9001 ctaaaatagc attcgctatt tcttttaaaa gtacagtttt accagtttta ggttgtgcta
     9061 caattaagcc tcgttgtcct ttcccaatag gacagaataa atcaattatt cgagtagata
     9121 aggtagaatt acgtccatta agtataaatt tttcttctgg aaacaagggt gtaagatgtt
     9181 caaaagggac tctatctctt acaaatgcag ggtccctacc atttattttt tcaactttca
     9241 caagaggaaa gtatttttct ccttccttag gaggacgaat atatccgtct acaaaatctc
     9301 cagtttttaa accaaacaat cgaatctgtg attgcgacac atatacatca tcaggagacg
     9361 gcatgtaatt atattcagct gaacgcaaaa aaccataatt ctctaccatt tccaatacgc
     9421 ctgtaccttt caatatatta tcaaattcat acagcttttc agaattttca ttcgagtgat
     9481 taggtttagt aataactctt tgttcttttt ttattacaac atttggttgt gtcgtaacgg
     9541 gcagatcaac cccagtgttt tcttcttgct tttcgtcaac aacaactgta tttacagtac
     9601 ttatagaaga tataacagag actttttctt ctgcctgttg attatctatt tgaatagatt
     9661 ctactgtttt tttcagtaaa gtaggaggag ttggtttttt agtgattacc ttttttttag
     9721 gtacttctac tatttgttgt tttaatgcat cttctttatg tacatctggt ataagttcaa
     9781 ctactgaaac tggcgtttct atacttttat catctgcttt ttcattactc ttacctacag
     9841 gctttgtaac tcgtgccctt ttttcttttc gagcaactac tgttgtaggt tttgttactg
     9901 ttttttgtgc cactttctca gcttctttat caagtatcgc atatactaaa gcttgttgtt
     9961 ctaagctaga tattttaggc acagataact ctttggctat cctttgaagt tctgaaagag
    10021 tttttttctc taattcattt tttgttgaca tgtaaaaagg agtgttttgt aacgtaacta
    10081 aatacttttg tctataaaca aaagtctgtg tataaaaatg tgtaggattg ttttaagtgc
    10141 a
//
LOCUS       DAOSRP010000070         9840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6652_length_9840_cov_5.300562, whole genome shotgun sequence.
ACCESSION   DAOSRP010000070 DAOSRP010000000
VERSION     DAOSRP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_9840_cov_5.300562"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /gene="porU"
                     /locus_tag="PLJ82_05560"
     CDS             complement(<1..2313)
                     /gene="porU"
                     /locus_tag="PLJ82_05560"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOR41008.1"
                     /translation="MKLYSFFLVFLLFSTLSAMSQQEHRFSIAIDWKKVQDLRANDEV
                     QRRLFFEGANYLGENQLPYFTYTIPISKEGTLSTYTVALDNAVFTSSTDDETTVLSQL
                     GVPVQTTIHIDYCIVYEKKQPSLQLLFTPIIQKENSYAKLIKADLIISSTPANKTGNQ
                     GKRSLGDEDQSIHQYASSSVLASGNWYQIRVPQTGIYKLTYEDLLSLGIQNPAQVRVF
                     GYGGAILEEDFSIPRVADDLPEIALFMEKGSDDVFNAGDYILFHAQGTIRWQYDKYSG
                     YYKHQQNYYATHGYYFVTERAGEGKRITSQPLVTETPVATVTDFLQYGVYEKEQVALL
                     QSGRKWYGDKFTNTNRVLTIPFSFPSVSTTKSAKIDISVVGNSTQSSYISVHIDDVEQ
                     TNKMSIFAITATNQVAREVNSTYYHMPKATSEINVKLTYAPENATAFAHLDFVRVNAY
                     QALKMQGNSMYFRQTDQNVRYDVLQYELGNATSDIRIWDLSDPLNMVEIPTTFSTNTL
                     FFVPSVRSDLLVREFLAIRTSATFPKPEVIGKIANQNLHAIQQVDYVIISPNEFREQA
                     MRLATFHQQKNGYSVVIVEPQQIYNEFSSGTPDATAYRWFLKMLYDRAGDNINNQPKA
                     LLLFGLSSHDNRGLVYSRMPLLSYQSFNSFDSHISYTTDDYYAFLDDSEGVNIIQDIM
                     DVGVGRLPVATKEEAKIVVDKIINYANNTKKGSWKNLLAFLGDDGDSNDHMEQSDNLV
                     QKWQEKWNEYQPIKVFLDSYQMTQTASGSTY"
     gene            complement(2324..2938)
                     /locus_tag="PLJ82_05565"
     CDS             complement(2324..2938)
                     /locus_tag="PLJ82_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOR41009.1"
                     /translation="MKILAVGWNYAEHNQELGQTLPTEPIIFSKPDSALLLGNKPFFM
                     PNFSNEIHYETELVVRINRLGKNIAPRFASRYYSEVALGIDFTARDLQRSLKAAGQPW
                     EISKAFDNSAAVSEFIPLSSLSVSIQELSFYLQLNDKIVQQGNTKDMIFSVDTIIAYV
                     SQFFTLKIGDLIFTGTPAGVGQIAKNDHLQGYLQGNRLLNFYIK"
     gene            3052..3414
                     /locus_tag="PLJ82_05570"
     CDS             3052..3414
                     /locus_tag="PLJ82_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOR41010.1"
                     /translation="MKNNDWKTKLGIVFSTNPEFGQTEEEEEEPTLPIHSVNPSKQTL
                     RIEIDKKGRNGKCATLITGFIGDDVALKELAKKMKTSCGVGGSTRDGEILIQGDVRTK
                     IDELLRSEGYKTKLITGK"
     gene            3431..6655
                     /locus_tag="PLJ82_05575"
     CDS             3431..6655
                     /locus_tag="PLJ82_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOR41011.1"
                     /translation="MNNLLVYQASAGSGKTFKLTENYLRLLFSEPDAYKRTLAVTFTN
                     KATEEMKTRILLELHKLADNQESDYIPIFCDRNNPFDVLALRKKAKEILQTILHDYSR
                     FKVSTIDRFFQQIIRSFIREIGLNNNYILEVDNKKILIETVETMMFQLEDKKHKKLLE
                     WLISLAEQRIESGQNWSFKNEIVTLGTEIFKEHYLRFSSELEKVTEKEFLHDYITSLK
                     NLKKTVKQTLQQKATHILDIIANNGLSTADFKRYTLDALEHFAKNDIRELTATFLKLE
                     SDTTAYCTKNSPNKDRINSLVSNGLHELITDFIRTHQSIILQYNTAEVIIKYSNILGI
                     ITDIHTCIKENNNENNALLLSDSSNLLHSIINDSDTPFIYEKIGTYIDHYLLDEFQDT
                     SRLQWENFKPLISNSLANGNTNLIVGDVKQSIYRWRSSDWKILAQQLDKEFPQYVQHE
                     SLLDNWRSDEEIISFNNKLFLFATQQLQAKYVDDIAKAGIDTTNNSLTHLLTESYAQV
                     QQNFPKKKEYRKNKGYIEIQFTDRNNKEEFEAYTLLQMVKNIEKIQESGYSLRDIAIL
                     TRTKAEGKVVAEYLLEYKNNPLANTGFAYDIISEESLCIANSKIVQFIIAILLYQLEP
                     SDKINRLKIVYLYNNLFHPTKSIVDEYLSKKDIENSSVYDIFSDDEKTSIISSIHLPL
                     YEMTEKLIELFTLNQFYEDIVYLQAFQDLILDFITQKGSDLRSFLAWWEETGKEKTVS
                     TSDTQNAIRIITIHKAKGLDFGFVLLPFATWNVDHIAHNSTILWCKPTEAPFDMLPIV
                     PISYSSNLERTLFQNNYFEEKMYAYIDNINTLYVALTRAKFGIFATGSHSKKPEENKT
                     IGDLLYTFCNSSIQKGKENTTEEEKYCFGRWEEMLLPSKKQIDNQQYQVSTYFSADPS
                     SKISLRLHASDFIGISPAKDYGKLMHQILEHITCKNDIHISVLKLRMEGKITDKESED
                     IRSQLELFFTLTEVKEWFSGIYKSYNERSILTADGKKYRPDKILTHANKAIVIDYKFG
                     EQVVYSHEKQLMTYMQLLKEMHFTDINGYIYYGQTNTLQSYSIT"
     gene            6662..7357
                     /locus_tag="PLJ82_05580"
     CDS             6662..7357
                     /locus_tag="PLJ82_05580"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOR41012.1"
                     /translation="MNYTKIGIIVAMNSEFTLVSELLTNKEHFQHKHLHFCTGNLGKK
                     EVILLKSGIGKVNAAIATTIMIQEFRPDCVLNTGVAGGIDKRLQVADIVVGQKTCYHD
                     FYAGEVQNHQAELGFPDEIVANKDLLATLKSKLQYNKKFSFGLICTGDQFITNNDELM
                     HIKKKRPCGLAVDMESNSIAQVCFYHQLPFISFRIISDTPWVDNHQQQYDNFWEEAPQ
                     KTFQLLKLLLAEL"
     gene            complement(7359..8372)
                     /locus_tag="PLJ82_05585"
     CDS             complement(7359..8372)
                     /locus_tag="PLJ82_05585"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR41013.1"
                     /translation="MKEMNCLVTGSTGLVGAHLLAHLAQSHLYTVRALKRKTSKMDIL
                     RYVFGFYSQDIDSLLDTITWIEGDILDKDSLAIAMEGIQVVFHCAADVVIGEDITGSV
                     LKTNVQGTQNIVDVALQKKIQILCYVSSVAAIGKSITDSYPDENALWDSNQPHSVYAQ
                     SKYETEQVVFAIPRNQMRVIVVNPGVILGVNNNKHGSSAVIYLAKKGFPFYTQGGSGY
                     VDVRDVCKAMLLLVQSSVTHERFILVGENCTTKQLLTYFAQGFQHTPPFILMPKWLLC
                     IFAWCAEILSTILKAKGNFNRKFIQTALHTSFYSAEKMKQRFNFHFTPMHQCIEDICK
                     YIN"
     gene            8460..9167
                     /locus_tag="PLJ82_05590"
     CDS             8460..9167
                     /locus_tag="PLJ82_05590"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOR41014.1"
                     /translation="MHHFYCPDIHSSLQLTEEESAHAIRVLRLGIGDEIEVFDGNGHI
                     CTAIIEQAHPKHTSVRITQTSHIPKNRNYTIAIAIAPTKNMDRLEWFVEKACEIGIDR
                     ITPIICHYSERKICKTDRLHKIIISAIKQSHQAYMPILDETMTFDEWLSTVHAEQKCI
                     AHCYQDATKIELTKKDVINKNISLAIGPEGDFSEKEIKRALQKGFTSVSLGENRLRTE
                     TAGIVACHTIHVLNQLV"
     gene            complement(9182..9376)
                     /locus_tag="PLJ82_05595"
     CDS             complement(9182..9376)
                     /locus_tag="PLJ82_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41015.1"
                     /translation="MVIAKRFFLLIVAYQVASASAFAQLMPDTSADKDALSSWELWLT
                     VFVAVLVLGIIQYVKYKNKD"
     gene            complement(9543..>9840)
                     /locus_tag="PLJ82_05600"
     CDS             complement(9543..>9840)
                     /locus_tag="PLJ82_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41016.1"
                     /translation="TTTKAQTFMWKVKLVQGVITQIIQTHGNGIDVTSINRQVYITGL
                     QSEATIQVYDMLGKLVQVIQNVQNNQALTINNSGVSILNVSTFTQQTQAKVFVK"
BASE COUNT         3527 a         1740 c         1553 g         3020 t
ORIGIN      
        1 atatgtactt ccacttgcag tttgcgtcat ttgatatgag tctaaaaaca ctttaattgg
       61 ttgatattca ttccatttct cttgccattt ttgcactaaa ttgtcagact gttccatgtg
      121 gtcattggaa tcaccatcat cccctaaaaa agcaagcaaa tttttccaag atcctttttt
      181 tgtattgttg gcataattga taattttatc taccactatt ttagcttctt ctttagttgc
      241 gacaggcaga cgacccaccc caacgtccat aatatcttgt atgatattta ccccctctga
      301 atcatccaaa aaagcataat aatcgtcagt tgtataagaa atatgagagt caaaagaatt
      361 gaatgattga taactcaata aaggcatacg agaatacaca agcccacgat tatcgtgaga
      421 tgataatccg aaaagcaaca aagccttagg ttgattgttg atattatctc cagctctatc
      481 atacaacatt tttagaaacc atcgatacgc tgtagcatca ggtgttcctg aagaaaattc
      541 attataaatc tgctgaggtt ctactatcac tacagaataa ccattttttt gttgatgaaa
      601 tgtagccaaa cgcattgcct gttctctaaa ttcatttgga gaaataatta cataatccac
      661 ttgttgaatc gcatgtaaat tttgattggc tattttacca attacttctg gttttggaaa
      721 tgtagccgac gtgcgtatag ccaaaaattc tcgcaccaac agatcagagc gaacagaagg
      781 gacaaaaaac agcgtattag tcgagaatgt agttggtatt tctaccatat tcagaggatc
      841 ggataaatcc cagatacgta tatccgaagt agcatttcct aactcatact gcaatacatc
      901 ataccgtaca ttttgatctg tttgcctaaa atacatacta ttcccttgca ttttaagagc
      961 ttgataagca tttacacgaa caaaatcaag atgagcaaaa gcggttgcat tttcaggagc
     1021 ataagtaagt tttacattaa tttcggaagt cgcttttggc atatgataat aggtactatt
     1081 tacttctcgt gcaacttgat tggtagccgt aatagcaaaa atagacattt tattagtttg
     1141 ttccacatca tctatatgca cacttatgta actactctga gtagaatttc ccaccactga
     1201 aatatcaatt ttagctgatt tggttgtact tacagaaggg aatgaaaagg ggattgttag
     1261 taccctattt gtattggtaa atttatctcc ataccatttc ctccctgatt gaagtaaagc
     1321 tacttgttct ttctcataca ctccatactg taagaaatcc gttaccgtag caacaggagt
     1381 ttctgtaaca agaggctgag atgttatgcg tttgccttct cctgctcttt ctgttacaaa
     1441 ataatatcca tgggttgcat aataattctg ttgatgttta taatacccac tatacttatc
     1501 gtattgccag cggatagtac cttgtgcatg aaacaaaata taatctcccg cattaaacac
     1561 atcatcagat cctttttcca taaatagtgc aatttctggc aaatcgtcag ctacacgagg
     1621 aattgaaaaa tcttcttcca agatagctcc accataacca aaaacacgca cttgagccgg
     1681 attttgtata cctagtgaca acaaatcctc ataggttaat ttataaatac cagtctgtgg
     1741 cacacgaatc tgataccaat taccagaagc caatacagat gacgatgcat attgatggat
     1801 agattgatct tcatcaccta atgatcgttt tccttgattc ccagttttat ttgcaggagt
     1861 agaagagata atcaaatctg ctttaataag ttttgcgtaa gaattttcct tttgaataat
     1921 tggagtaaaa agaagttgaa gtgatggttg ttttttttca taaacaatgc aataatcaat
     1981 gtgtattgtt gtctgtacgg gtactcccaa ttgggataac acagttgttt catcatccgt
     2041 agaagaagta aaaacagcat tatcaagggc aacggtatag gtagaaagtg ttccttcttt
     2101 gctaatggga attgtatacg taaaatatgg taattgattc tcccccaaat aattagctcc
     2161 ttcaaaaaat aagcgtcttt gaacctcatc attcgcccgc aagtcttgca cttttttcca
     2221 gtcgatcgca attgaaaaac gatgttcttg ttgcgacatt gcagatagtg tcgaaaacaa
     2281 caaaaagacc aaaaagaaac tatataattt catggatgaa tatttatttg atataaaaat
     2341 taagcaatcg gttaccttgc aaataacctt gcaaatgatc atttttagca atttgaccta
     2401 ctcccgctgg cgtacctgta aaaattaaat caccaatttt aagggtaaaa aattgactca
     2461 catacgcaat tatagtatca acggaaaaaa tcatgtcttt agtattccct tgttgcacaa
     2521 ttttgtcatt aagttgcaaa taaaatgata actcctgtat agaaacactt aatgagctta
     2581 atggaataaa ttcgcttacc gcagccgaat tgtcaaacgc tttgctaatt tcccaaggtt
     2641 gaccagccgc ttttaatgaa cgttgcaaat cgcgagcagt aaaatcaata cctaaagcta
     2701 cttcgctata ataacgcgaa gcaaatcgtg gtgctatatt ttttcctaaa cgattaattc
     2761 gtaccactaa ttctgtttca taatgaattt cattggagaa attgggcata aaaaaaggtt
     2821 tattccccaa caacaatgca gaatcaggtt tgctaaatat aataggttcg gtaggtaaag
     2881 tttggcctaa ctcttgatta tgctccgcat aattccatcc tactgcaagt attttcattt
     2941 atctgttttt tttctaataa atatccgttg tactaattgt ggatatttta caaaggtaat
     3001 atttttttta ttcagttagt tttgtttctt ttgtaataaa atttaaaata tatgaaaaac
     3061 aacgattgga aaactaaatt gggtattgta ttttctacaa atcctgaatt tgggcaaaca
     3121 gaagaagaag aagaagagcc tactctacca attcattcag taaacccatc caaacaaaca
     3181 ttgcgtatag aaattgacaa aaaagggaga aacggaaaat gtgccacatt aattacaggt
     3241 tttattggcg atgatgttgc cctaaaagaa ttggcaaaaa agatgaaaac aagttgtggt
     3301 gttggaggtt caacacgtga tggagaaata ttaattcagg gagatgtgcg taccaaaatt
     3361 gatgaactcc tacgttcaga agggtataaa acaaaactta ttacaggaaa ataattagca
     3421 aaaaaaaggg atgaataatt tactggttta tcaagcttcg gcaggttcag gaaaaacatt
     3481 taaacttact gaaaactatc tacggttact ttttagtgag ccagatgcct acaaacgaac
     3541 actggctgtt acctttacca acaaggcaac agaggagatg aaaacacgta ttttgctaga
     3601 attacacaaa ttggctgaca accaagaaag tgattatatt cctatttttt gcgataggaa
     3661 taaccctttt gatgtattgg ctttacgaaa aaaagctaag gaaatattac aaacaatact
     3721 acatgattat tcacggttta aggtaagtac cattgatcgt ttttttcagc aaattattcg
     3781 ctcctttatt cgcgaaattg gattaaataa caactatata ctagaagtcg acaacaaaaa
     3841 aattctaatt gaaacagtcg aaacgatgat gtttcaatta gaagataaaa aacataaaaa
     3901 attactcgaa tggcttatct ctttagcaga acaacgcatt gaaagtggtc aaaactggtc
     3961 atttaagaat gaaatcgtaa cactaggtac agaaatattt aaagaacact acttgcgttt
     4021 tagttccgaa ttagaaaaag ttactgaaaa agaattttta catgactata ttacctcttt
     4081 aaaaaatcta aaaaaaactg ttaaacaaac gctacaacaa aaggctacac atattttgga
     4141 tattattgca aataatggac ttagcacagc tgatttcaaa agatacacac tggatgcatt
     4201 agaacatttt gcaaaaaatg acatccgcga attaacagcc acttttttaa aactcgaaag
     4261 cgatactact gcttattgca caaaaaatag cccaaacaag gatagaatta attcattagt
     4321 aagcaatggg ttgcatgaac ttatcacgga ttttatccgc acgcatcagt ctattatatt
     4381 gcaatacaat accgccgaag ttataataaa atacagtaat attttaggaa ttattaccga
     4441 tatccacaca tgtattaaag aaaataataa cgaaaataat gctttactat taagcgatag
     4501 ttccaatcta ttacatagca tcatcaatga tagtgatacc ccttttattt acgaaaaaat
     4561 tggcacatat attgatcact acctactaga tgaatttcag gacacttcaa gactgcaatg
     4621 ggaaaacttt aaaccactta tatctaatag tttagcaaat gggaacacca acttaatagt
     4681 aggtgatgta aaacaaagta tatatcgttg gcgtagttct gattggaaaa tattggctca
     4741 acaacttgac aaagaatttc cacaatacgt tcaacatgaa tctttgctag ataattggcg
     4801 aagcgacgaa gaaattattt ctttcaataa taagcttttt ttatttgcca cacagcagtt
     4861 acaagcaaaa tatgtagacg atatagcaaa agctggcatc gatacaacaa acaattcatt
     4921 aacacatttg ttgacagaat catacgctca agtacaacaa aattttccta agaaaaaaga
     4981 atatcggaaa aacaaagggt atatagaaat tcagtttaca gatagaaaca acaaagaaga
     5041 attcgaggct tatacattgc ttcaaatggt taagaatatt gaaaaaatcc aagaatcggg
     5101 ttactctctt cgtgatatag ctatacttac tcgaacgaaa gctgagggca aagtggtagc
     5161 tgaatattta ctagaatata aaaataaccc gctagccaac actggctttg catacgatat
     5221 tatttcagaa gaatccttgt gtattgctaa ttcaaaaata gttcaattta ttattgcaat
     5281 tttgctatat caattggaac cttcagacaa aataaatagg ctaaaaatag tatatttata
     5341 caacaatctg tttcatccaa ccaaatcaat agtagatgaa tacttatcca aaaaggatat
     5401 tgaaaacagt tcggtatatg atatattttc tgatgacgaa aaaactagta taatttcgtc
     5461 tatacacctt cctctttacg aaatgactga aaagttaatc gaactattta cactcaacca
     5521 gttttacgaa gatatagtgt acttacaagc ttttcaagat cttatactcg actttattac
     5581 acaaaaagga tcagatttac ggtctttttt agcctggtgg gaggaaacag gcaaagaaaa
     5641 aaccgtatct acctcagata cgcaaaatgc tatccgaata ataaccattc acaaagccaa
     5701 aggattagat tttgggtttg tgctgctgcc ttttgcaaca tggaatgtgg atcatatagc
     5761 tcataattca actattttgt ggtgcaaacc cacagaagct ccttttgaca tgttgcccat
     5821 cgtaccaata agttatagtt ctaatctaga acgtacccta tttcaaaata attatttcga
     5881 agaaaaaatg tatgcgtata ttgataatat caacacatta tatgtagctc ttacacgagc
     5941 aaaattcgga atatttgcta caggatcaca tagtaaaaaa ccagaagaga acaaaacaat
     6001 aggagattta ttatatactt tttgcaattc atcaatacaa aaaggcaaag aaaacacaac
     6061 agaggaagaa aaatattgct ttggtagatg ggaagaaatg ctactcccta gcaaaaaaca
     6121 aatagataac caacaatatc aagtttctac ctatttttca gccgacccat cttcaaaaat
     6181 ctcgttacga ttacatgctt ctgattttat aggcattagt cctgcaaaag attatggaaa
     6241 attaatgcat caaatcttgg aacacattac atgcaaaaat gatattcaca tatcagtatt
     6301 aaaattacga atggaaggga aaatcactga taaagaatca gaagacatcc gctctcaact
     6361 ggaacttttc ttcacattaa cagaggtaaa agaatggttc tctggcattt ataaaagcta
     6421 taatgaacgt agtattttaa ctgctgatgg taaaaaatac cgaccagaca aaatactcac
     6481 acatgccaat aaagccattg taattgatta taaatttgga gaacaagttg tatatagcca
     6541 tgaaaaacaa ttaatgacat atatgcaatt attgaaagaa atgcatttta ctgacattaa
     6601 tggatatatt tactacggac aaacaaatac actacaatca tactcaataa cataaaccaa
     6661 catgaattac acaaaaatag gcattattgt tgccatgaat agcgagttta cattagttag
     6721 cgaactgctt acaaataaag aacattttca acataaacac ttgcactttt gtacaggaaa
     6781 ccttggcaaa aaagaagtaa tactgcttaa aagtggtatt ggcaaagtaa atgctgccat
     6841 agctaccaca attatgattc aagaatttcg tccagattgt gtattaaaca caggtgtagc
     6901 tggtggtatt gataaacgct tacaggtagc cgatattgta gtggggcaaa aaacatgtta
     6961 ccacgatttt tatgccggtg aagtgcaaaa ccatcaagca gaacttggat ttcctgatga
     7021 aattgttgca aataaagatt tacttgcaac attaaaatca aaactacagt ataataaaaa
     7081 gttttctttt ggattaattt gtacgggaga tcaatttatt actaataatg atgaacttat
     7141 gcacattaag aaaaaaagac cgtgtggatt ggcagtagat atggagtcta acagtatagc
     7201 tcaagtatgc ttctaccacc aattgccatt tataagtttt cgcattatta gcgatactcc
     7261 ttgggtagat aaccaccaac aacaatacga taatttttgg gaagaagctc cacaaaaaac
     7321 ctttcaatta ctcaaattac tgttagcgga gttataaatt agttaatata cttacaaata
     7381 tcttctatac actggtgcat tggtgtaaag tggaaattaa aacgctgttt catcttttct
     7441 gcactataaa aactcgtatg caaggccgtc tgtatgaatt ttctattaaa atttccttta
     7501 gctttcaaaa tggttgatag tatctctgca caccaagcaa aaatacacaa taaccactta
     7561 ggcatgagaa taaaaggagg ggtatgctga aatccttgtg caaaatatgt gagcaattgt
     7621 tttgttgtac aattttctcc tactaaaata aaccgttcat gcgtaacact actttgcacc
     7681 aacaatagca tagctttaca tacatcacgc acatctacat agccactacc accttgcgta
     7741 taaaaaggga atcctttttt ggctagataa attactgcac tactaccatg tttattatta
     7801 tttacaccaa gaataactcc cggattcaca acaataacac gcatttgatt acggggtatg
     7861 gcaaacacta cttgttctgt ttcatattta ctttgagcat atacagaatg tggttgatta
     7921 ctatcccata aagcattctc atctggataa ctatccgtta tcgatttgcc aatagcagcc
     7981 acagaactga cataacatag aatttgtatt ttcttttgca atgctacatc tactatattt
     8041 tgagttccct gcacattggt tttcaatacc gaacctgtaa tatcttcacc aatcactaca
     8101 tctgcagcac aatgaaatac gacttgaata ccctccattg caattgccaa actatcctta
     8161 tctaatatat ctccttctat ccaagtaatg gtatccaata atgagtctat atcttgagaa
     8221 taaaacccaa aaacataacg taatatgtcc attttactgg ttttacgttt taatgctcgc
     8281 acagtatata ggtgcgattg tgccaaatga gccaacaaat gtgctcctac taatccagta
     8341 ctaccagtaa ccaaacaatt catctctttc atatttgtgc aaagataatt aaaaaggaat
     8401 aacaatactt gtcaagttgc tttcaaaatg ctatttttgc acctaaaata agaaaaacta
     8461 tgcatcattt ttattgtcct gatattcatt cgtcattaca acttaccgaa gaagaatctg
     8521 ctcatgctat tcgtgtatta cggttgggta ttggcgatga aatagaagta ttcgatggta
     8581 atggtcatat ttgtacagcc attatcgaac aggctcatcc aaaacatact tctgtgcgta
     8641 tcacccagac ctcacatata ccaaagaata gaaattatac tattgcaatt gccatagctc
     8701 ctacaaaaaa tatggatagg ttagaatggt ttgtagaaaa agcatgcgaa ataggcatag
     8761 atagaattac acccataatt tgccactatt cggaacgtaa aatatgcaaa acagatagat
     8821 tgcataaaat tattatttcg gcaatcaagc aatctcacca agcttatatg ccaattttag
     8881 atgaaacaat gacatttgat gaatggctat caactgtaca cgccgaacaa aaatgtattg
     8941 ctcattgcta ccaagatgct acaaaaatag aattgacaaa aaaagacgtt atcaataaaa
     9001 acatctcact agcaatagga ccagaaggag attttagtga aaaagaaata aaaagggctt
     9061 tacagaaagg ctttacaagt gtttcattag gtgaaaacag actacgcaca gaaactgctg
     9121 gaattgtggc atgccatacc attcatgttt taaatcagct agtttaaaaa aacgacctaa
     9181 atcaatcttt atttttatat ttaacgtact gaataatacc taaaactaac acagctacaa
     9241 agactgttag ccataattcc caacttgaca atgcatcttt atcggctgat gtatctggca
     9301 ttaactgagc aaatgcagaa gcacttgcaa cctgataagc tacgataagc agaaaaaatc
     9361 ttttagcaat aaccatattt acactattag catttagtgg tacatttttt atattgctac
     9421 aaagataaaa cttttatacc tcaaacatca aagttttatg ttgtttttct cttattttac
     9481 aaaaaaaatt acccaattaa cattgaaaaa gggatgaaga aatagagatt ctgcaagaaa
     9541 gactatttta caaagacttt ggcttgtgtt tgctgagtaa aggtactgac attcaatata
     9601 cttacaccag aattattaat agttaaagct tgattatttt gtacattttg tataacttgt
     9661 actaatttgc ctaacatgtc gtatacctga atagtcgctt cactttgtaa gcctgtaata
     9721 tatacctgtc tgttgataga tgttacatca atgccatttc catgagtttg aataatttgc
     9781 gttataactc cttgcaccaa ttttactttc cacataaagg tttgagcttt tgtggtggta
//
LOCUS       DAOSRP010000071         9698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6770_length_9698_cov_5.040133, whole genome shotgun sequence.
ACCESSION   DAOSRP010000071 DAOSRP010000000
VERSION     DAOSRP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_9698_cov_5.040133"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLJ82_05605"
     CDS             <1..795
                     /locus_tag="PLJ82_05605"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOR41017.1"
                     /translation="WSIKGNTGINFSQASFVNWVAGGDNSFTGTAYFNVRASYIQGRS
                     AWNTDLSLEYGGLWLIDNNEYRKNSDKIDFATKYGYAMNKTLFASGLFDFKTQFSEGY
                     DYKTSPATITSRFVAPAYSKLSIGLDYKPTKVFSLFMSPITGKMTFVSDNSLAIKYGM
                     VDENNVILNPFWFKIEPGAFLKADVNWDITSNINLNTKADFFTAYDNSFGDIDINWDL
                     VVSMKVNQFIRTSISTSLKYDKDILIEGTPKVQFREIIALGIGYSF"
     gene            complement(884..2047)
                     /locus_tag="PLJ82_05610"
     CDS             complement(884..2047)
                     /locus_tag="PLJ82_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR41018.1"
                     /translation="MIQQDNLLSFLLNSINKIILSTGFANVEIGNIIMIIIGIMFIYL
                     AIKKGWEPMLLVPIGFGMIVGNIPFLEGSDIGIKEEGTVLNILYGGVRYGWYPPLVFL
                     GIGAMTDFASLISNPKLLLIGAAAQFGIFGAYALALAFGFPANEAGSIGIIGGADGPT
                     AIFVTSILSPHLLGAIAVSAYSYMALVPVIQPPVMRLLTTKKERLIHMKKPRAVTQTE
                     KVVFPIMGLLLTTFIVPSGLPLLGMLFFGNLLKESGVTNRLADTAKGPMIDIVTILLG
                     FTVGASTQAAFFLTTKSIFIFMLGAFSFIIATAAGVMFVKIFNLFLKGENKINPLIGN
                     AGVSAVPASARVSHMMGLKYDPTNYLIMHAMGPNVAGVIGSAVAAGIILSFLS"
     gene            complement(2052..2498)
                     /locus_tag="PLJ82_05615"
     CDS             complement(2052..2498)
                     /locus_tag="PLJ82_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOR41019.1"
                     /translation="MKEYNFIINGTKYKVGIQSVEDNIAEVEVNGTPFYVEMEKPLVK
                     KGIKPLAKRAVYTHDETTVSSPSQPVVKPVVKVKTGALLSPLPGVITAISVNVGDTVK
                     IGQKVLALEAMKMENNINADKDGKVLEIKVSKGDSVLEGTELIIIG"
     gene            complement(2592..3461)
                     /locus_tag="PLJ82_05620"
     CDS             complement(2592..3461)
                     /locus_tag="PLJ82_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOR41020.1"
                     /translation="MRLSITLLLLLSLFSVNMSAKSLNLVINELLVTNTENLVDDYGN
                     RSGWIEIYNPTHRVIDIGGCYLTNDTLNPVKYKIPEGDEITLVAPLSHIIFYADNKTH
                     FGIFHTNFTLQESNYIALYDRDQTTCLDSVVYPLTMPANFSYGRLKDGEPEWGMLAKT
                     SPANNNAFVDKGAASKVFAQQDPYGVVLTIASMSVVFLALLLLYTLFSLTAKFSIYVT
                     KERIRKTAKGEKSETIVETHIPAEVYASIAMAIHEYQEEIHDVEDTILTIQRVAKTYS
                     PWSSKIYGLRNFL"
     gene            complement(3485..5041)
                     /locus_tag="PLJ82_05625"
     CDS             complement(3485..5041)
                     /locus_tag="PLJ82_05625"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOR41021.1"
                     /translation="MSHQLEKIAELIALREKARLGGGEKRIENQHKKGKYTARERIAM
                     LLDEASFEEIDMFIKHRCTNFGMDKKEAILGDGVVTGCGTIDGRLVYVFAQDFTVEGG
                     SLSETMALKICKVMDLSLKMGAPIIGINDSGGARIQEGVTALAGYAEIFQRNILTSGV
                     VPQISAIFGPCAGGAVYSPALTDFTIMTKGTSYMFLTGPKVVKQVLGEDVTQEELGGA
                     SIHSTKSGVAHFAVDSEEEGILCIRKLISFLPQNNLEEPPLVSCSDPIDRLEDSLNDI
                     IPDSPNKPYDMYEVLGSVIDNGEFLEVHKDYAPNIIVGFARFNGTSVGIVANQPKVLA
                     GVLDSNSSRKAARFVRFCDAFNIPIVTFVDVPGFLPGTQQEYNGVILHGAKLLYAFGE
                     ATVPKITITLRKSYGGAYCVMSSKHLRGDVNYAWPTAEIAVMGPEGAIEVLYGKEIAA
                     SDNPTELQKEKVEEYKELFANPYNAARYGYIDDIIEPRNTRFRIIRSLQQLATKKLTN
                     PLKKHDNLPL"
     gene            complement(5069..5485)
                     /gene="mce"
                     /locus_tag="PLJ82_05630"
     CDS             complement(5069..5485)
                     /gene="mce"
                     /locus_tag="PLJ82_05630"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOR41022.1"
                     /translation="MKPKKIEHIGIAVPSIAEALPFYENVLGLTCYAVEEVADQKVKT
                     AFIMVGEVKIELLEATSPESPIAKFIEKNGGKGGVHHIAFAVDQLADKLKIAEEKGVQ
                     LIDKAPRKGAENMSIAFMHPKSTLSVLTELCEAPKE"
     gene            complement(5498..6574)
                     /locus_tag="PLJ82_05635"
     CDS             complement(5498..6574)
                     /locus_tag="PLJ82_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41023.1"
                     /translation="MVYIVLFLLLLALMNVYFSLGRKYGIVAHVNERSSHTKPVITGA
                     GIIFVVSYLFYLVYASWKGIHVNWFISIAALLIAIVSFFDDIKEIWFLFRLCFQIVAV
                     ILLMWQLKIDFSISFLGLKMLDWIAFSFLLILFVAAVNLYNFIDGINGLIAGLSLVFF
                     FSVIFIDYYVIDFIENREKIYFLIVPTIIFSLYNFRKKPVCFCGDVGSIFLGIIVVYF
                     LASLIIKTNNFTYVLLFSLIIIEAGYTVIQRLLMGQNIFKPHRIHLFQLLCNQYGHSH
                     LKVTLFYVLIQTVLSLFVVLANYYDLSITWQYSVGAVVFFMLSLSYVLYKRKLQGGHL
                     LEGGEPIRKLRKIVYKKESNIYIK"
     gene            complement(6589..7848)
                     /locus_tag="PLJ82_05640"
     CDS             complement(6589..7848)
                     /locus_tag="PLJ82_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOR41024.1"
                     /translation="MKNLKFRLIVMSFLQFFVWGAWLITIAIYWFDTKQWDGAQFGAV
                     FSTLGISSLIMPTLMGIIADRWVNAEKLYGLLHLLSGLCIFSLAFVNSPVVFFWVMLL
                     AMFFYMPTISLSNSIAYNALKEVNLDIEKAFPPIRIWGTIGFIIAMWVVNLTGNKATA
                     VQFYISAVLSFCLAIYSFTLPACIPQRSKEKSSSIVQMLGLDAFKLFANPKMAIFFIF
                     SMFLGAALQLTNMYGDTFLDDFKNIEQYADSFVVRYSTIVMSISQISETLFILAIPFF
                     LKRYGIKNVMLMSMIAWVLRFALFGFGNPDDRLWMIILSMVVYGMAFDFFNISGSLFV
                     ETSTDKKNRSSAQGLFMMMTNGFGAIFGSLASGYIIDTYFIQADGLKNWQGIWLTFSG
                     YALVITILFALIFRYKHTTNDVLKIKH"
     gene            complement(7854..8411)
                     /locus_tag="PLJ82_05645"
     CDS             complement(7854..8411)
                     /locus_tag="PLJ82_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOR41025.1"
                     /translation="MIELYVFGVSLGISKTETHTLLLKEVAGSKYLMIEIGYSEAQSI
                     AIQLEQKKVPRPLIYDVIVNLVSNLNVAIEHIVIDKLQDGIFFSKLVYRMGVHVLQSD
                     CRPSDGVAIAVRCGCPIFVSEEVLTVAMQQRLAIKTKKLADMTYEELKQEMQLAAERE
                     DYKQALLCKEEIIKRSTNNEKKNPN"
     gene            8577..9143
                     /locus_tag="PLJ82_05650"
     CDS             8577..9143
                     /locus_tag="PLJ82_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOR41026.1"
                     /translation="MYKELIKTIALFIAKPVKAWEYINTKNPTENNPLHSYAYPLMGL
                     GSLSAFIVALVNNGSNDGFQIALKEASLLFISSFIGLYTAAFLIRIFYEKYCKEPLSF
                     DVTIQFVVYSSSVIFIINLFNQVTSSIFFLQIFSLYTIYIVWEGSIHFLKIEESKRAS
                     FVLFSAFCMILLPIAITALMKFFMPGLQ"
     gene            9155..9619
                     /gene="smpB"
                     /locus_tag="PLJ82_05655"
     CDS             9155..9619
                     /gene="smpB"
                     /locus_tag="PLJ82_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOR41027.1"
                     /translation="MKTKQTAIRIKNKRATFDYEILERYTAGVVLVGTEIKALREGKA
                     NLTDSFCFFFNNELWVKNLSISPYFYGSYNNHNPRRDRKLLLTTKELRKLERGTKEKG
                     CTLIPTLLFINEKGLAKLEIALAKGKKEYDKRDSIKEKEDKRVMDRAFKSKI"
BASE COUNT         3418 a         1805 c         1388 g         3087 t
ORIGIN      
        1 tggagcataa aaggaaatac cggaattaat ttctctcaag ctagttttgt caattgggta
       61 gcaggtgggg ataattcctt tacaggcact gcctatttca acgtaagagc gtcttacatt
      121 caaggacgtt cggcttggaa taccgattta tcactagagt atggggggtt atggttgatt
      181 gataataatg aataccgaaa aaattcggat aagattgatt ttgctactaa atacggttat
      241 gcgatgaaca aaacactttt tgcatcagga ctgtttgatt ttaaaaccca attttccgaa
      301 ggatatgatt ataagacatc tccagctact attacatcac gatttgtagc accagcgtat
      361 tcaaaactgt ctattggtct tgattataaa ccaacaaaag ttttttcttt atttatgtct
      421 cctataacag ggaaaatgac ttttgtaagc gacaactctc ttgccattaa atatggaatg
      481 gttgatgaaa acaatgttat tcttaatcca ttttggttta aaattgaacc aggagctttt
      541 ttaaaagctg atgtaaattg ggacattacg tcaaatatca atttaaatac caaagctgat
      601 ttctttaccg cctatgacaa tagttttggt gatattgaca ttaactggga tttagtagta
      661 agtatgaaag taaatcaatt tattcgtaca agtattagta cttccttgaa atatgacaaa
      721 gatattttaa tagaaggtac tccaaaagtg caatttagag aaattattgc cttaggtatt
      781 ggatattctt tttaaataaa gagaaagtct ataataaaat tgggttgaga aattctatct
      841 caacccaatt ttattatatc tgaaactaga tagtaagctc ttgttatgac aagaaactta
      901 aaataatacc cgcagcaact gctgaaccta tcactcctgc cacattaggt cccattgcgt
      961 gcataatcaa gtaattggta ggatcatact tcaaacccat catatgagaa actcttgcag
     1021 atgcaggcac agctgaaaca ccagcattac caataagtgg attgattttg ttttcgcctt
     1081 ttaaaaataa attaaaaatc ttaacaaaca tcacacctgc agcagtagct ataataaacg
     1141 aaaaagctcc caacataaaa ataaaaattg atttagtcgt caggaaaaac gccgcttgag
     1201 tggatgctcc aacagtaaaa cctaacaaga ttgtaacaat atcaatcata ggacctttgg
     1261 cggtatctgc taatcgattg gttaccccac tctcttttaa caaattccca aaaaatagca
     1321 tccccaataa tggcaatcct gatggaacaa taaaagttgt taataataat cccattatag
     1381 gaaaaaccac tttttccgtc tgagtaacag cccttggttt tttcatatga ataagacgct
     1441 ctttcttggt tgtaagcaac ctcattactg ggggttgaat aacaggcact aaagccatat
     1501 acgaataggc tgacacagct attgctccca ataaatgtgg ggataatata gacgttacaa
     1561 aaatagctgt tggaccatca gcaccaccaa taatacctat tgaacctgct tcattagcag
     1621 gaaatccaaa cgctaaagcc agtgcataag caccaaatat accaaattgt gcagcagctc
     1681 caattaataa caattttgga ttagagataa gagatgcaaa atcagtcatt gctccaatac
     1741 ctaaaaatac taaaggagga taccaaccat atctaacccc accataaaga atatttaaaa
     1801 ctgtaccttc ttcttttata ccaatatctg aaccttctaa aaaaggaatg tttccaacaa
     1861 tcataccaaa accaatagga actaaaagca ttggttccca tcctttttta atagctaaat
     1921 agataaacat gattccgata ataatcatga taatattacc tatttctaca tttgcaaacc
     1981 ctgtactcaa aataatctta ttgatactat tgagcaaaaa tgaaagtaaa ttatcttgct
     2041 gtatcattat tctatcctat tataattaat tcagttcctt ctaaaacaga atccccttta
     2101 ctaactttta tttccaatac tttgccatct ttatcggcat tgatattgtt ttccattttc
     2161 attgcctcta gagctaatac tttttgccca attttaactg tatcacctac attgacacta
     2221 atagctgtaa taactccagg taatggggat aaaagtgctc ctgtttttac tttcactaca
     2281 ggttttacta cgggttgaga aggagagctc actgttgttt catcatgcgt atatactgca
     2341 cgttttgcaa gaggtttaat acctttttta acaagaggtt tttccatttc tacgtaaaaa
     2401 ggagtgccat taacctctac ttctgcaata ttgtcctcta cgctttgtat accaacttta
     2461 tatttggtac catttataat aaaattatat tctttcatct attttattta ttaaaaaatt
     2521 catatatagt aaacgatata actgattgta tatttaccag ttattttttc tatacagctt
     2581 gttgattttt tttacaaaaa attacgcaac ccatatattt tagaactcca aggagaatac
     2641 gttttagcaa cacgttgtat agtcaaaata gtatcttcaa catcatgtat ttcttcctga
     2701 tattcgtgta tagccatggc aatagacgcg tatacttctg ctggaatatg tgtttcaaca
     2761 attgtttcac ttttttctcc cttagctgtt ttacgaatac gctctttggt tacgtatata
     2821 gaaaactttg ccgtaagaga aaataatgta tacagtagta gtagtgcaag aaacacaaca
     2881 gacatagaag ctatagttaa tacaactcca tatggatctt gttgtgcaaa aaccttacta
     2941 gcagctcctt tatctacaaa ggcattatta ttagctggag atgttttagc caacataccc
     3001 cattcaggtt caccatcttt caatcgtccg taggaaaaat tagcaggcat agtaagagga
     3061 tagactaccg aatctaaaca cgttgtttga tctctatcat acaaagcgat ataattactt
     3121 tcttgtaatg taaagtttgt atgaaatatt ccaaaatgag ttttattatc tgcataaaat
     3181 attatatgag ataatggagc aacaagcgtt atttcatctc cttcaggtat tttgtatttt
     3241 acaggattaa gggtatcatt tgtcaaatag caacccccaa tatcaatcac acgatgggta
     3301 ggattatata tttctatcca accacttcta tttccataat catccaccaa gttctcagta
     3361 ttcgttacta ggagttcatt tataaccaag tttaaacttt ttgcagacat gttaacagag
     3421 aacaaactaa gaaggagtaa cagcgtaata gataaacgca tttttatata gttatatatc
     3481 ataattataa aggcaaatta tcatgctttt taagcggatt agttaatttt ttggttgcca
     3541 attgttgcaa tgaacgaata atccgaaaac gcgtattacg tggttctata atatcatcaa
     3601 tataaccata tcgtgcagca ttatagggat tagcaaacaa ctctttgtat tcttctactt
     3661 tttctttttg tagttcagtt ggattatcag aagcagctat ttcttttccg tacaatacct
     3721 cgatagctcc ttcaggtccc atcacagcaa tttctgctgt aggccacgcg taatttacat
     3781 ctcctcgcaa gtgtttagaa ctcatcacgc aataagctcc accatacgac ttacgtaggg
     3841 ttattgttat ttttggcaca gtagcttcgc caaatgcata aagcaattta gcaccgtgca
     3901 aaataacacc attgtattct tgttgtgtac caggtaaaaa tccaggaaca tctacaaatg
     3961 ttacaatagg aatattgaag gcatcacaaa aacgcacaaa acgtgcagct ttacgagatg
     4021 aattactatc caaaacacca gctaacacct taggttgatt agcaacaata cctaccgaag
     4081 ttccattaaa acgagcaaaa ccaacgataa tattaggggc ataatctttg tgtacttcta
     4141 aaaattcgcc attatcaatt acagaaccta atacctcgta catatcataa ggcttatttg
     4201 gagagtcagg tataatatca ttaagcgaat cttccaatct gtcaattggg tcagagcatg
     4261 acaccaaagg aggttcttct aaattatttt gaggcaaaaa gctaattaat ttgcgaatac
     4321 acaaaatacc ctcttcctcc gagtcaacag caaaatgagc tactcccgac tttgtagaat
     4381 gaatacttgc accacctagt tcttcttgtg taacatcttc tcctaacact tgtttcacaa
     4441 ctttaggtcc agttaaaaac atataactag tacctttggt cataatagta aaatcagtta
     4501 aggctggaga ataaacagca ccaccagcac atggaccaaa aatagcagat atttgaggca
     4561 ctacacctga tgttaaaata ttgcgttgga aaatttctgc ataaccagct aatgcagtta
     4621 ctccctcttg aattcttgct ccaccagagt catttatgcc tatgataggt gctcccattt
     4681 tcaaagacaa gtccataact ttgcaaatct tcaatgccat tgtttcagac aatgatcctc
     4741 cttcaactgt aaaatcttgg gcaaacacgt ataccaatcg cccatcaatg gtaccacatc
     4801 ctgttaccac cccatctcca agaatggctt cttttttgtc cataccaaaa ttggtacaac
     4861 gatgcttaat aaacatatct atttcctcaa aactagcttc atcaagtaac atagctatac
     4921 gctctctagc tgtatacttg cctttcttat gttggttttc gatacgtttt tctccgcctc
     4981 ctaatcgtgc tttttcacgt agagcgatta attctgcaat tttttcgagc tgatgactca
     5041 tagtatatat tttttaaagg ttatgaaatt attctttagg tgcttcacac aattcggtta
     5101 aaacactgag agtagatttt ggatgcataa atgcaatact catattttct gcacctttac
     5161 gaggagcttt atctattaat tgcactcctt tttcttcagc tatttttaat ttatccgcta
     5221 attggtcaac agcaaaagct atatgatgta cccctccttt tccaccgttt ttttcaataa
     5281 atttagctat agggctttct gggcttgtcg cttctaataa ttcaattttt acttcaccaa
     5341 ccataataaa agctgttttt actttttgat cagctacttc ttcaactgcg taacaggtta
     5401 atcctaatac gttttcataa aaaggcaaag cctcggcaat acttggaaca gctatgccga
     5461 tgtgttcaat tttttttggt ttcatttttt taaaatttta tttaatatat atgttagatt
     5521 cttttttgta aactattttg cgcaactttc gtataggttc gcctccttct aataaatgtc
     5581 ctccctgcaa ttttcgtttg tataatacat aactcaggct caacataaaa aagaccacag
     5641 ctcctacact gtattgccaa gttatactca aatcataata attagctaaa acgacaaaca
     5701 aagaaagtac agtctgtatc aagacataaa aaagtgtaac ctttaaatga ctatgcccgt
     5761 attgattgca taataactga aataagtgta tgcgatgtgg tttaaaaata ttttgtccca
     5821 tcaataagcg ttgaatgaca gtatatcctg cctcaataat aatgagagaa aataacaaaa
     5881 catacgtaaa gttgtttgtc tttataatca atgatgcaag aaaatacacc acaattatac
     5941 ctaaaaaaat tgaaccaaca tccccacaaa agcaaactgg tttttttcta aaattataca
     6001 atgaaaaaat aattgttgga acaattaaga aataaatttt ctctctattt tcaataaaat
     6061 caatcacata atagtcaata aaaatgacag agaaaaagaa cactaaactt aaacccgcaa
     6121 tcaaaccatt aatcccatca ataaaattat ataaatttac agcagctaca aaaagtataa
     6181 gtaaaaaaga aaaagcaatc caatctaaca ttttcaatcc taaaaaagaa atagagaaat
     6241 ctattttcaa ttgccacatc aacaaaataa cagctactat ttgaaaacac agccgaaata
     6301 aaaaccaaat ttctttgata tcatcaaaaa aactaacaat ggcaattaac aaagctgcaa
     6361 ttgaaataaa ccaatttacg tgtatcccct tccacgaagc atagactaaa taaaacaaat
     6421 aggatacaac aaaaataata ccagctcctg ttatcacggg ttttgtatga gaacttcttt
     6481 cattaacatg tgctacaata ccatattttc ttcctaacga aaaatacaca ttcattaatg
     6541 caagtaaaag taagaaaagg actatatata ccatctgatt tctctatgtt aatgtttaat
     6601 ttttaataca tcattggtgg tatgtttgta tctaaatata agagcaaaca atattgtaat
     6661 aaccaaagca tacccagaaa atgttaacca tataccttgc caatttttta atccatctgc
     6721 ctggataaag taagtatcaa taatgtatcc acttgccaag ctaccaaata ttgcaccaaa
     6781 tccattggtc atcatcatga aaagaccttg tgcactggaa cgattttttt tatcagtaga
     6841 tgtttcaaca aacaacgagc ccgaaatatt gaaaaaatca aaagccatac catatactac
     6901 cattgataga attatcatcc ataagcgatc atctggattt ccaaaaccga acaatgcaaa
     6961 acgtaatacc caagcaatca tactcattag cattacattc tttataccat agcgttttaa
     7021 aaagaaaggt atagctaaaa taaacaatgt ttccgatatt tgtgaaatag acattacaat
     7081 ggttgaatac ctaacgacaa aagagtcagc atattgttca atatttttaa aatcgtctaa
     7141 aaacgtatct ccatacatat tagtaagttg aagtgctgcc cctaaaaaca tcgaaaaaat
     7201 aaaaaagata gccattttag gattagcaaa taatttaaac gcatctaaac caagcatttg
     7261 tacaatggat gaagattttt ctttacttct ttgcgggata catgcaggta acgtaaatga
     7321 atagatggct aaacaaaaag ataaaactgc tgaaatataa aattgtactg ctgttgcttt
     7381 gtttccagtt aaatttacca cccacatagc aataataaaa cctatcgtac cccaaatacg
     7441 tatgggtgga aatgcttttt caatatcaag atttacttct ttcaaagcat tatatgcaat
     7501 agaattactt aatgaaatgg ttggcatata aaaaaacatg gcaagcaaca ttacccaaaa
     7561 aaatacaaca ggtgaattta caaacgcaag agaaaaaata cataacccac ttaacaagtg
     7621 tagtaagcca tataattttt ctgcattaac ccatctatca gcaataatcc ccattaatgt
     7681 aggcattatt aaagaagaaa tacccaaagt ggaaaatact gccccaaatt gagccccatc
     7741 ccattgtttt gtatcaaacc aataaatagc aatagtaata agccatgccc cccacacaaa
     7801 aaattgcaga aaactcatca ctatcagtct aaatttaaga tttttcatat ttcttaattt
     7861 ggattttttt tctcattatt agtagagcgt tttattatct cctctttaca taacaaagct
     7921 tgtttataat cttcacgttc agcagctaat tgcatttctt gttttaattc ctcatacgtc
     7981 atatcagcca atttcttggt cttaattgct aatcgttgtt gcattgctac tgtcaatact
     8041 tcttcagaga caaatatagg acaaccacaa cgaacagcaa tagcgacccc atcagatggt
     8101 ctgcaatcac tctgcaaaac atgtacaccc attctataaa ctaatttcga aaaaaaaata
     8161 ccatcttgaa gtttatcaat aacaatatgt tcgattgcaa cattaagatt gctcactaaa
     8221 tttactatta catcgtatat gagaggtcgt ggtacttttt tctgttcgag ctgaatggca
     8281 atagattgtg cctcactata tcctatttct atcatcaaat acttacttcc tgcaacttct
     8341 tttaacaata gtgtatgagt ttctgtttta gaaattccaa gactaacgcc aaaaacatat
     8401 aactcaatca tttgcaagca ttttatttta ggctgacaaa gttaagaaat aaaaatcaaa
     8461 aaagaaaaaa gaaaaatgcc ttttatataa gaaaaaacaa aaaatagggt taattcatat
     8521 tttactaaaa aaaatagtat ttttgtgtcc cgtaacaacg aaaaaaaatt agaacaatgt
     8581 acaaagaatt aattaaaact attgcattat ttatagctaa acctgtaaaa gcttgggaat
     8641 acattaacac aaaaaatcct actgaaaata atccactaca ttcttatgcc taccctttaa
     8701 tgggtttagg ctcgttatct gcttttatag tggctctcgt taataacggt agcaacgatg
     8761 gatttcaaat tgcactaaag gaagcctcac tattatttat ttcaagtttt attggcttgt
     8821 atactgccgc atttctaatt cgtatttttt atgaaaaata ttgcaaagaa cctctttctt
     8881 ttgatgttac cattcaattt gttgtatata gctcttctgt aatttttatt attaacttgt
     8941 ttaatcaagt tactagtagc attttctttc tacaaatatt ctctttgtac actatatata
     9001 tagtatggga aggaagcatt cattttttaa aaatagaaga atcaaaaagg gctagttttg
     9061 ttcttttcag tgctttttgt atgatccttt taccaatagc aatcacggca ttaatgaaat
     9121 tctttatgcc cggtttacaa taagttaatc aaccatgaaa accaaacaaa ctgccattcg
     9181 gataaaaaac aaacgagcta cgtttgatta tgaaatttta gaacgctaca cagcaggcgt
     9241 tgtacttgta ggtacggaaa taaaagcttt acgagaagga aaagccaatt taaccgattc
     9301 tttttgtttc ttttttaata atgaattatg ggttaaaaac ttgtcaattt ctccctattt
     9361 ttatggatcg tacaataacc ataaccctcg aagagaccgc aaattattgc tcaccacaaa
     9421 agaactccgt aaactagaac gtggcacaaa agaaaaaggt tgcacgctaa tccccacttt
     9481 attatttata aatgaaaaag gattagctaa actagaaatt gctttagcaa agggaaaaaa
     9541 agaatacgac aaacgagata gtattaagga aaaagaagat aaacgtgtta tggacagagc
     9601 ttttaaatcg aagatataaa aaaagcctta cgaaacgtaa agcttttgta gcgggaacga
     9661 gagttgaact cgtgacctcc gggttatgaa tccgacgc
//
LOCUS       DAOSRP010000072         9687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6790_length_9687_cov_4.906146, whole genome shotgun sequence.
ACCESSION   DAOSRP010000072 DAOSRP010000000
VERSION     DAOSRP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6790_length_9687_cov_4.906146"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLJ82_05660"
     CDS             <1..667
                     /locus_tag="PLJ82_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HOR41028.1"
                     /translation="ILHALTEEFGMVSYMIYNPWSKQAKVKASYLQTFSLLEIDVEHQ
                     LKKDIQRIHDAKILFVGNSIHTNPYKQSIVLFLAEMVRKTLSETEKDVHLFAFVYHSL
                     CVLDEVTEKYNSFHLIFLMRLTQFLGFYPNSTVENNDVYFDMLNGIFTAQPPLHGHYL
                     EGENCKHFQALTHISYTDMPAWNISRVDKLTLLSTMVRYYQLHIPTIQRIKSLDILHS
                     LFV"
     gene            727..798
                     /locus_tag="PLJ82_05665"
     tRNA            727..798
                     /locus_tag="PLJ82_05665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:760..762,aa:Arg,seq:cct)
     gene            827..3193
                     /locus_tag="PLJ82_05670"
     CDS             827..3193
                     /locus_tag="PLJ82_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41029.1"
                     /translation="MRKNGSSLHPSPLFSISLRVICVITLLFLSHISVVAQQVFSISG
                     TISDKATGETLTGATIYPKSNPSSAVYSNAYGYYALSLAKGTHTIVVNYIGYSSLEYS
                     IEVQENIRKNFQIQPASTQLDEVVVSAIKRNETILSDKIGVEKLEIQSIQKIPVLFGE
                     QDILKTLTLTPGVKTMGEGSGGMYVRGGNNSHNLILLDESMVYNANHLLGFFSTFNGD
                     AIKDVTLYKGTAGAEYGGRLASVMDVRMKDGNNQHYHVSGGIGLISSRISVEGPIIRN
                     KGSFLLTARRTYADLFLKLSSDPLLNNNQLYFYDINAKANYQLNDNNRLFISAYAGRD
                     VFSFFDRFNMNWGNITATVRWNHIFNNRLFSNTSFIYSDYGYKVGINLAENSFSLLSQ
                     INNVQLKHDYQYFLTAQNTLSFGIQATHHRITPGQLSTESTLFQPFKLQEKFAFENNI
                     YITNNWKPNYHWNVSYGVRGNIFSLLGGGDFYTYLNDGTIQTSFYDYNQFVKTYLYIE
                     PRINIAYILNDANSFKMSYTRNTQNLHLITNSTSSVPTDIWIPSSKNIKPQISDQVST
                     GYFVHFLDDTYQVSSEIYYKWMQNQLDLKNGANIQANEYIENELLVGNGRAYGLEIML
                     KKQYGALSGWIGYTLSRTENQIEGINNGNWYPARHDATHDVSVVAIYELSKKWTLSST
                     WVYNTGNAVTFPSGKYQVDGETILLYTNRNASRMPHYHRMDIGATRYFKKSKRFESSL
                     NFSVYNAYGRKNAFTIDFEQDENDPDKINAVKTYLFTFMPSITYNFKF"
     gene            3193..3999
                     /locus_tag="PLJ82_05675"
     CDS             3193..3999
                     /locus_tag="PLJ82_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOR41030.1"
                     /translation="MRKIIWLLVISFGITSCTEIIQLDLQNNKSEVVVEASMAAANEI
                     RVLLSRSVNLDESNTFPLLTGAQVSISDENNNTEVLTEIEPGVYQSITTKGIEGKRYF
                     LDLIVNNQSISATSTMPSIVPFTDMVVEKGSIFGPIGGNTNTDNTEYYEIKVLFNDPV
                     EEVNYYRFVEFINNEKQQEYIYDDRLNNGKDVEIVLFRFDRLLKTEDVVRIEMQTITK
                     EFYVYLSSLNDLRNSARSGSTPANPVTNMQGTSLGYFNTYTSQSKEYIIE"
     gene            complement(4001..4804)
                     /locus_tag="PLJ82_05680"
     CDS             complement(4001..4804)
                     /locus_tag="PLJ82_05680"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR41031.1"
                     /translation="MVKYIDLDVAIQNSNSAFFKKMPKWVISLLKKIIHQDEMNRILN
                     KYEDCYGVDFLPKIIEELQLTVQIEGEENLPADGRCFFAANHPFGFVDGLILTNTVGK
                     KYGKLKAIGNDSFLLAPHLKPIIAAVNVFGSNPKAYLLELENLFESDSPITHFPAGVV
                     SRIRNGKIEDKDWQKSFIKQSVKYNRPIVPFHFTGRNSYLFYIIYLIRSFLGIKLNIE
                     LMLLPYEIFNKKGKTIHVNIGKPIMPETFDTSKTSAEWAQYVKQQVYSL"
     gene            5044..5952
                     /locus_tag="PLJ82_05685"
     CDS             5044..5952
                     /locus_tag="PLJ82_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR41032.1"
                     /translation="MPTISVIITTFNGSKNINNTLRAILQQEGINSLFELDIIAIDDC
                     STDNTLEILNEYPIRIFSTETNSGGPNKGRNIGLANAKGDYICIVDQDDVWHTNRISS
                     VLPYIQKAPIISSGYTLIDTSTGKTIERVAQSKEPFIFFEKNSTFLSKLTRSSQGQNT
                     YLGSLLFHRSLRNILFEEHFGKIDYDWVLRLFHQQTSIEICSSLYTRIVDGTNLSLNP
                     TYRRQDFYYSLYFIEQFENLYPTQTRIARKKIHGSRARYYYIMNDMRRARVYFRLAGF
                     SFNNILYYCTTFAGSSFVKKQFNVFG"
     gene            6007..6723
                     /locus_tag="PLJ82_05690"
     CDS             6007..6723
                     /locus_tag="PLJ82_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOR41033.1"
                     /translation="MHSHFRKTYLYKNTNPDLVKHLKPTITCPKKWYGTSYGGFYINP
                     NLLTNQSVIYSFGIGKDISFDMACSNHHQSSIYAFDPTPKSIKWIKEQKLPANFHFFD
                     FGISVGDQEFAAFYLPKNTKSVSGSTIQSDVFDTNNSIKLPMKSFAKITEMLHHTHID
                     VLKIDIEGSEYEVLEDILTTKISINQLLIEFHDRLFDMQTYKSVEIVKKLNEAGYHIF
                     GCSKSFEEVSFIHASVLKNT"
     gene            complement(6742..8235)
                     /locus_tag="PLJ82_05695"
     CDS             complement(6742..8235)
                     /locus_tag="PLJ82_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOR41034.1"
                     /translation="MKSYLQHIPLGTSLPVSNPHAISVSFPSMDDVIGYEKKDVCTIQ
                     QLQSGYPRFFRNKLVHLLCETVRKEFKLPVSVEIYPLPCNALLSLLNLYFCKDFVKIE
                     AYGVTFLLLDENDVQLDKLRGFLRQVGVFMSSRNAEDILHSNGLISTTFNEAYTEVQP
                     YYFVQQSLQKAYETNSVDDIILSNCGMNAFFAVFQTIQDISLLSGKNRVLQLGNLYLD
                     SYDILRTYANKITLSHVTDFDEIRRMIQMHHTVLSAVYVEVPNNPLLECVDLPRLYAL
                     CQEYNIVLLVDSSMASAHTLNLLPYCDVITESLSKFANGQGDVLMGAIVVNAASTYSK
                     EIKSRIASYVIPPYVRDVQRLAHSISDYYERVCLSSASTRKLIEYLQNSPEVEQVYSV
                     LHPLSKRNFSILQKNDSLIPSVFSVLFKQPLAYYYDKLPLPKGPSFGTVFTLAMPYVY
                     LAHFDLIQTVEGRQQLTEMGLHPDMLRISVGLEPVEDIIASFKLAGI"
     gene            complement(8262..9326)
                     /gene="aroB"
                     /locus_tag="PLJ82_05700"
     CDS             complement(8262..9326)
                     /gene="aroB"
                     /locus_tag="PLJ82_05700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOR41035.1"
                     /translation="MSQQRLVFCSTIADSIVAELENKPSTSIFILTDSQSKSFCLPLL
                     QQSETLKNAHVIEVPAGDGSKDVNTLVSIWQYLSENGATRKSLMINLGGGMITDLGGF
                     AASTFKRGMNYVNVPTTLLAMVDAAVGGKTGINFNGLKNEVGVINPAKSVLIDTNFLR
                     TLDMQNLLSGYAEMIKHALISSESELKNIFELDFAQLDYEKLNQLVQSSIYIKEAIVT
                     EDPQEEGVRKALNLGHTIGHAFESYSYQMNKPALHGYAVAWGLMCELFLSYKKLGFPK
                     DTLIKVARFVKENYGHFYISCDDYDALYELMLHDKKNTIANCINFSLLSNVGVLSLNQ
                     HIDKETIFESLDFFQEMMGM"
     gene            complement(9387..>9687)
                     /locus_tag="PLJ82_05705"
     CDS             complement(9387..>9687)
                     /locus_tag="PLJ82_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOR41036.1"
                     /translation="LRAHSEAIWAETERPISVEEAQAAFAKATGVILQDNPAQKEYPM
                     PLFVAGKDPVYVGRVKKDLANPNGIFFWTVSDQIKKGAALNAVQIAEYLIAEGNI"
BASE COUNT         3351 a         1835 c         1571 g         2930 t
ORIGIN      
        1 tattctgcat gcactaacag aagaatttgg catggtatcg tacatgattt ataatccatg
       61 gagcaaacaa gccaaagtaa aagcatcgta cctacaaaca tttagtctgc tagaaattga
      121 tgtagaacat caacttaaaa aagatattca gcgtatacat gatgccaaaa tactattcgt
      181 aggcaatagc atacatacca atccttacaa gcaaagtatt gttctctttt tagccgaaat
      241 ggtacgtaaa acattatcag aaacagaaaa agacgttcac ttatttgctt ttgtctatca
      301 ttcgttgtgt gtattagatg aagtaacaga aaaatacaat tcttttcacc ttattttctt
      361 aatgcgatta actcaattcc taggttttta cccaaatagt acagtagaaa ataatgatgt
      421 gtattttgat atgctaaacg gaatatttac tgcacagcca cctttgcatg gtcattattt
      481 agaaggagaa aactgtaagc attttcaggc acttacgcac ataagctata ctgacatgcc
      541 tgcttggaat atatcgcgtg tagataaatt gacattacta tcaactatgg tacgttacta
      601 tcaattgcat ataccgacca tacaacggat aaaatccttg gatattctcc attctttgtt
      661 tgtatgatgt aaaaaaatag tttgtttctt actggaaaaa atagtacttt tgctcatcaa
      721 atttttgcct ctgtagttca atggatagaa caaaagtttc ctaaacttta gatttgggtt
      781 cgattcccag cggaggtacg atgtccctaa ttcaggctat gtacttatga gaaaaaacgg
      841 ttcttccctt catccttctc ctcttttctc tatcagcctt agggttatct gtgtgataac
      901 actcctattt ctttcacaca tttcagtggt agcccaacaa gttttttcta tcagtggcac
      961 tatttccgac aaagcaacag gtgaaacatt aacaggagct acaatttatc caaaaagcaa
     1021 tccttctagt gctgtatatt caaacgcata tggctattat gccctttcgc tggctaaggg
     1081 cactcatact attgtggtaa attatattgg atatagcagc ctagagtact ccattgaggt
     1141 acaagagaat attcgaaaga attttcaaat ccaaccagct agcacacaat tggatgaggt
     1201 agtcgtatca gcaattaaaa gaaacgaaac tattttatca gacaaaattg gtgtagaaaa
     1261 gcttgaaata caatctattc agaaaatccc cgtactgttt ggtgaacaag atattcttaa
     1321 aacactaaca ttaaccccag gcgtaaaaac catgggagaa ggaagtggag gcatgtatgt
     1381 gcgaggtggt aataattcgc acaatttaat tcttttagac gaatcgatgg tatataacgc
     1441 gaaccatttg ctcggttttt tttccacatt caatggagat gctatcaaag atgttacttt
     1501 gtacaaaggt acggctggtg ctgaatatgg aggtagacta gcctctgtaa tggacgtaag
     1561 aatgaaagat ggcaacaacc agcactacca tgtaagtggt ggcatcggac ttatttcttc
     1621 tcgtataagc gtagaaggtc caattatacg caacaaaggc tcgttcctat taactgcccg
     1681 acgaacgtac gctgatttat ttctcaaact atcgagcgat ccgcttttaa acaacaacca
     1741 actatacttt tacgacatca acgcaaaagc aaactaccaa cttaatgata ataatcgcct
     1801 attcatatcg gcatatgctg gtagagatgt gttcagtttc ttcgaccgat ttaatatgaa
     1861 ttggggaaat attaccgcca cagtacgttg gaatcatatt tttaacaatc gattatttag
     1921 caatacttcc tttatctatt ctgactatgg ctataaggta ggtatcaatt tagctgaaaa
     1981 ctcgtttagt ttattatcgc aaataaataa cgtacaactt aaacacgatt atcaatattt
     2041 tctgacagct caaaacacac tctcatttgg tattcaagca acacatcatc gtatcactcc
     2101 agggcaattg agcacagaat ctaccctatt tcaaccattt aaactacaag agaaatttgc
     2161 ctttgagaat aatatataca ttaccaacaa ctggaaacca aattaccatt ggaatgtatc
     2221 ttatggagta agaggaaata tattctccct tcttggagga ggcgattttt atacatatct
     2281 caacgatggc actattcaaa caagctttta tgattacaat caatttgtaa aaacatatct
     2341 atatatagag ccacgaatca atattgcata cattttgaat gatgctaact cctttaaaat
     2401 gagctataca cgcaatacgc aaaatttgca cctcatcaca aactccacat catccgtacc
     2461 tacagatata tggataccct caagtaaaaa catcaaacca caaattagcg accaagtaag
     2521 cactggctat tttgtgcact ttttagatga cacctatcag gtatcgtctg aaatatatta
     2581 caaatggatg caaaaccaac ttgatttaaa aaacggagca aacattcaag caaacgaata
     2641 tatcgaaaac gaattattag taggcaacgg aagagcgtat ggattagaaa taatgttgaa
     2701 aaaacaatac ggtgcactct ccggatggat tggatacacc ttatcaagaa cagaaaatca
     2761 aattgaaggg ataaacaacg gcaattggta ccctgccaga cacgatgcta ctcatgatgt
     2821 ttctgtggta gctatttacg aactaagtaa aaaatggaca ctatcaagca cgtgggtgta
     2881 taatacgggc aatgcggtta ctttcccaag tggcaagtac caagtagatg gagaaactat
     2941 tttgctctat acaaacagaa acgcatctcg catgccacac taccatcgaa tggatattgg
     3001 agctactcgc tattttaaaa agtcgaaacg atttgaaagc agcctcaatt tttctgttta
     3061 caatgcttat ggaagaaaaa atgcttttac cattgacttt gaacaagatg aaaatgaccc
     3121 tgacaaaatc aacgcagtaa aaacatacct atttacattt atgccatcaa taacctataa
     3181 ttttaaattc tgatgaggaa aataatatgg cttttggtta tttctttcgg tatcacttcg
     3241 tgtacagaaa taatacagct cgacttgcag aacaacaaat cggaagtagt ggtagaggca
     3301 tctatggctg cagcaaatga aatacgtgtt ttacttagcc gttcggtaaa tttagatgaa
     3361 tcaaacacat ttccgttgct aacaggtgct caggtgagta tatcagatga aaataacaat
     3421 acagaagtgt tgacagaaat tgaacctggc gtttaccaat caattacgac aaaaggtata
     3481 gagggaaaaa ggtatttcct tgacttgata gtcaacaacc aatcaatatc agctacttct
     3541 actatgcctt ctattgttcc ttttacagac atggtagtcg aaaaagggag tatttttggt
     3601 cctattggag gaaatacgaa tacagataac acagaatact acgaaataaa agtgctattc
     3661 aacgaccctg ttgaagaagt aaattattat cgctttgtgg aatttattaa taatgaaaaa
     3721 caacaagaat acatttatga cgatagatta aacaatggaa aagacgtaga aatagtattg
     3781 ttccgctttg atcgattgct aaaaacagag gatgtagtaa ggatagaaat gcaaactatt
     3841 accaaagagt tttacgtata cttgagttcg cttaacgact tacgtaatag tgctcggagt
     3901 ggctcaacac cagccaaccc agttacaaac atgcaaggca catcattggg ctatttcaac
     3961 acgtacacta gccaaagcaa agagtatatc atagaataag ttacagcgaa tacacttgct
     4021 gtttgacata ctgtgcccac tcagcggatg ttttagaagt atcaaaggtt tcaggcataa
     4081 tgggtttgcc aatgttcaca tgaatagttt tacctttttt attgaaaatc tcgtatggca
     4141 atagcattaa ttcaatattt agtttaattc cgagaaaact acgaattaag taaatgatat
     4201 aaaataaata agaattgcgt cctgtaaaat gaaaaggaac aatggggcga ttatacttaa
     4261 cagattgttt gataaagctt ttttgccaat ctttatcctc aatttttccg ttacgaatac
     4321 gcgacaccac acctgcagga aaatgggtaa tgggtgagtc tgattcaaac aaattctcca
     4381 actccaacaa atacgctttt ggattagacc caaatacatt tacagcagca ataatgggtt
     4441 taagatgcgg tgctagcaaa aaagaatcgt tacctatagc ttttaactta ccatactttt
     4501 taccaacagt attggtaaga attagcccat ccacaaaacc aaagggatga tttgcagcaa
     4561 aaaaacatcg tccgtcagct ggtaaatttt cttccccttc tatctgtaca gttaattgta
     4621 attcttcaat aatcttaggc aaaaaatcta caccatagca atcctcatat ttatttagga
     4681 tccgattcat ttcatcttgg tgtataatct ttttgagtaa agaaattacc cactttggca
     4741 tttttttaaa aaaagcagaa ttgctattct gtatagccac atctaaatca atgtatttta
     4801 ccatattaca ttaagaatca agtaaataat aatatttttc gatgcaccat tttagaacaa
     4861 attattcagc aaagatagta agatagtaaa aataaatgag gctaatctac tatatatttt
     4921 gtgtaaaatt tcataaatac acatatatac ctaacataat agtcaaatta caaacaattt
     4981 attcaagatt aatttattaa ttcaaattaa tcttgtactt ttactcagta aatattacaa
     5041 attatgccca caatttcagt tattattacg actttcaacg gatcaaaaaa tatcaacaat
     5101 acgcttcgag ctattttaca gcaagaaggt ataaatagtt tatttgaact tgatatcatc
     5161 gctatcgacg attgttcgac tgacaatacg ctcgaaatct taaacgaata tcctattcgc
     5221 attttttcta ctgagacaaa ctcaggcggt ccgaataaag gacggaatat tggtcttgct
     5281 aatgctaagg gtgattacat ctgcattgta gatcaagacg atgtatggca tactaaccga
     5341 attagtagcg tattaccata tatccaaaaa gctcccatca ttagctctgg ctatacactt
     5401 atcgacactt ctactggaaa aacaattgaa cgtgtggcac aatcgaaaga accatttatt
     5461 tttttcgaga aaaatagcac ctttttaagc aaactgacac gctccagtca aggtcaaaac
     5521 acctatcttg gttcactgct ttttcatcgt tctttacgta atattttgtt tgaagaacac
     5581 tttgggaaga tagactatga ttgggtattg cgtctgtttc atcaacaaac ttctattgag
     5641 atatgctctt cgttatacac acgcatagta gatggcacaa acctatcatt aaacccaacc
     5701 tataggcgtc aagacttcta ttattcgctt tattttatcg aacaatttga aaacctctac
     5761 ccaacacaaa cacgtattgc tcgaaaaaag attcatggtt cgcgagctcg ctactattat
     5821 attatgaatg acatgagacg agctcgtgta tatttcaggc tagcaggatt ttctttcaac
     5881 aatatactct actattgcac aacctttgca ggcagtagtt ttgttaaaaa acaatttaat
     5941 gtatttggat aatcagtaag aaaacatgaa aaaataaaag aattcatcaa caatagtcga
     6001 caaaatattc actctcactt tcgtaaaaca tatctttata aaaacacgaa tccggattta
     6061 gttaaacacc taaaacccac cataacgtgt ccaaagaaat ggtatggaac cagttatgga
     6121 ggtttttaca tcaaccccaa cttgcttaca aaccaatccg ttatttactc atttggcatt
     6181 ggtaaagaca ttagctttga tatggcatgt agcaaccatc accaatcatc gatatatgca
     6241 tttgatccaa cacctaaatc aataaaatgg attaaggaac aaaaattgcc tgcaaacttt
     6301 catttttttg attttggcat ctccgttggg gatcaagaat ttgctgcatt ctatttacca
     6361 aaaaacacca aaagcgtgtc gggaagtact attcaatccg atgtatttga caccaacaat
     6421 agcataaaac ttccaatgaa atctttcgca aaaataacag aaatgttaca tcatacacat
     6481 atcgacgtat taaagattga tatagaaggg tctgaatatg aggttttaga agatatttta
     6541 acaacaaaaa tctccatcaa tcaattgtta attgagtttc acgacagact atttgatatg
     6601 caaacatata agtcagtaga gatagtaaaa aaactgaacg aagcaggata tcacattttt
     6661 ggttgctcaa aaagttttga agaagtatca tttatacatg catctgtatt aaaaaataca
     6721 taaaattcgt acaaaatcac tctatatccc agcaagcttg aaagaagcaa taatatcctc
     6781 aacaggttct aatcctacag atattcgtag catgtcagga tgaagtccca tctccgttaa
     6841 ctgctgtctg ccctctactg tttgtattaa atcaaaatgg gctaaatata cataaggcat
     6901 agctaaagta aagactgtac caaagcttgg acctttgggc aaaggtaatt tgtcgtaata
     6961 ataggctaat ggttgcttaa acaaaacaga aaaaacactt ggtataagcg agtcattttt
     7021 ctgtagaatc gaaaaattac gcttagacaa tgggtgcaaa acggaatata cttgctctac
     7081 ttcgggcgaa ttttgcaaat actcaatcaa tttacgtgta cttgccgatg acaaacatac
     7141 cctttcgtaa taatcagaaa tcgaatgggc taatcgttgc acatctcgca cataaggagg
     7201 aataacataa gacgcaattc gagattttat ttctttacta tacgtactcg ctgcattcac
     7261 aactatagct cccatcagca catctccttg tccgttggca aacttcgata agctctctgt
     7321 tattacatca caatatggca aaaggttcaa agtatgggca gaagccatag aagaatcgac
     7381 caataaaaca atgttatatt cttgacacaa ggcatacaaa cgaggtaaat ctacgcactc
     7441 aagcaaggga ttattaggca cttctacata caccgcactt aacactgtgt gatgcatttg
     7501 aatcattcgt cttatttcgt caaaatcggt tacatgagac aaggtaattt tattagcata
     7561 ggtgcgtaat atatcataag aatcaaggta tagattaccc aattgcaata ccctattttt
     7621 accagataac aaactaatat cctgaatagt ttgaaataca gcaaaaaaag cattcatacc
     7681 acaattactc aaaataatat cgtctacaga gtttgtttcg taagcttttt gaagggattg
     7741 ttgtacaaaa taataaggct gtacttctgt ataggcttcg ttaaacgttg ttgaaattaa
     7801 tccattggag tgcaaaatat cctccgcatt acgcgaagac ataaagacac ccacctggcg
     7861 aagaaaccct cgcaacttat ctaactgaac atcattttca tctagtaata aaaatgtaac
     7921 tccatatgct tctatcttta caaaatcttt acaaaaatac aaatttagca atgataatag
     7981 agcattacat ggcaaaggat atatctctac actcacgggc aatttaaact ctttacgtac
     8041 tgtttcgcaa agcagatgca cgagcttatt tctaaaaaaa cgaggatatc ctgattgaag
     8101 ctgctgaatg gtacatacat ctttcttttc gtaaccaatt acatcatcca tgctaggaaa
     8161 agacacagaa atggcatgag gattgctaac aggcaacgaa gtacccaagg gtatatgttg
     8221 taaatatgat ttcattgtat aaaaacccct tgtataggaa attacatacc catcatttct
     8281 tgaaaaaagt cgagcgactc aaaaatcgtt tctttatcga tatgttgatt taatgacaac
     8341 acccctacat tagataatag gctaaaattt atacaattag ctattgtatt ctttttatcg
     8401 tgcagcatta gttcgtacaa tgcatcgtaa tcatcacacg aaatgtaaaa atgtccataa
     8461 ttctctttca caaaacgagc caccttaatt aaggtatctt ttggaaaacc taatttttta
     8521 taagaaagga acaactcgca catcaaaccc caagctacag cataaccatg caatgcgggt
     8581 ttgttcattt gatacgaata gctttcaaaa gcatgtccga tagtatgacc taaatttaag
     8641 gctttacgta caccttcctc ttgtgggtct tctgttacta tggcttcttt aatatatata
     8701 gaagattgca ccaactgatt gagtttttcg taatccaatt gagcaaaatc taattcaaaa
     8761 atattcttta attcactttc cgaagatatc aatgcatgtt taatcatttc tgcatagcca
     8821 gataacaaat tctgcatatc aagcgtacgc aaaaagtttg tatcaatcaa cacactctta
     8881 gcgggattaa ttaccccaac ttcattcttc aacccattaa agttaatacc cgttttacct
     8941 ccaactgcag catcaaccat agccaataag gtagtaggca catttacata gttcatacct
     9001 cttttaaacg tagaagcagc aaatccaccc aaatcggtta tcatacctcc tcctaaattt
     9061 atcatcaacg attttcgtgt agcaccattt tcgcttaaat attgccaaat agaaacaagc
     9121 gtgtttacat ctttacttcc atcgcctgct ggcacttcta taacatgagc attttttagt
     9181 gtttcgctct gttgtaacaa gggtaaacaa aaacttttgg actgactatc agtaagaata
     9241 aaaatagatg ttgagggctt gttttcaagt tcagccacaa tgctatctgc aatagtagag
     9301 cagaaaacta agcgttgttg cgacataatt agaatagtat taaaaactaa acgaatgctt
     9361 tttggcaccc gtttagtatc ttatgattaa atattacctt ctgcaattag gtattctgct
     9421 atttgaacgg cattaagagc tgcacctttt ttaatttggt cgctcactgt ccaaaagaaa
     9481 attccatttg ggttggctaa gtctttcttt acacgtccta catatacagg gtctttgcct
     9541 gctacaaaga gtggcattgg atattctttt tgtgcaggat tatcttgcaa aattacacca
     9601 gtagctttgg caaaagcagc ttgtgcttct tctaccgaaa taggacgttc ggtttctgcc
     9661 caaattgctt ccgaatgtgc tcttaac
//
LOCUS       DAOSRP010000073         9445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_6996_length_9445_cov_4.801917, whole genome shotgun sequence.
ACCESSION   DAOSRP010000073 DAOSRP010000000
VERSION     DAOSRP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6996_length_9445_cov_4.801917"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLJ82_05710"
     CDS             complement(<1..337)
                     /locus_tag="PLJ82_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR41037.1"
                     /translation="MNFPLYIAKRYLFSKKSHNAINIISLISASGVAVGTVALVCVLS
                     VFNGFQGVIEGLFGKFDAQLQVTVNEGKSFNPQISSVQSLYKMPEIKVICEVVQDNAL
                     LIFSDKQTPA"
     gene            complement(347..682)
                     /gene="rbfA"
                     /locus_tag="PLJ82_05715"
     CDS             complement(347..682)
                     /gene="rbfA"
                     /locus_tag="PLJ82_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR41038.1"
                     /translation="METKRQNKIARLIQKEISEILQSITKEMNQGVLITPTIVRVSPD
                     LGVAKIYLSIFPSEKSADMLAFVTERSQTIKHHLAGRTRFQLRTVPSLSFYLDDSLDY
                     IEHIDKLLK"
     gene            complement(731..1369)
                     /locus_tag="PLJ82_05720"
     CDS             complement(731..1369)
                     /locus_tag="PLJ82_05720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR41039.1"
                     /translation="MNISSQLDEYILQHSEQEPLLLQELYREAHVRLLRPRMVSGHLQ
                     GRILSMLSKMIQPKRILEIGTFAGYSALCLAEGLPADGKIITIEIDDEMEDFARSFID
                     KSPYKEQIELVIGDSLHLIPTMDEKFDLVFIDADKRDYCAYYDVVFDKVLSGGYIFAD
                     NTLWDGKVVEPLVPNDKQTAGVLAFNRYIAADTRVEKVVLPLRDGLTLIRKK"
     gene            complement(1376..2596)
                     /locus_tag="PLJ82_05725"
     CDS             complement(1376..2596)
                     /locus_tag="PLJ82_05725"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOR41040.1"
                     /translation="MNIQTHLFANNIRLLHVHDNSEVAYAGVIINTGTRDELPNEEGY
                     AHLTEHMLFKGTQKRNSTQIINRIEDVGGELNAYTSKEETVVYSVFLTKYLNSAVDVI
                     VDIVFNSIIAQHELQKEVDVVIDEIHSYMDSPSELIVDDFENLLFNQYAIGRNILGTE
                     ESLQKCTPESLKEFINRTYTTDEMVFFTLGNFSIEKVIRTLGKYIASIPATKRTYARE
                     TPIDYVPTRVTLEKKTLQAHYILGNRAYSMQEEKRFAFHLLNNIVGGQGMNSRLNMSL
                     REKSGLVYTVESHYTPYSDTGLWTVYFGTDHTDVPQALRLIYKEIKKVKENQLSPIRL
                     FKAKQQLIAQMLIAGENKENWIIAKAKHFLNFNSLMEQTEIEAKIMAISSSQLCDVAN
                     ECLQEEQFTHLHYM"
     gene            complement(2678..4234)
                     /locus_tag="PLJ82_05730"
     CDS             complement(2678..4234)
                     /locus_tag="PLJ82_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOR41041.1"
                     /translation="MKINLLQKVILFLTLIAFFACKNDKNVSNTTGWKYNDKKHSGFQ
                     VIHDYEQDTPPGMVLIEGGTFTMGRVTEDTYSEWNNYPRRVTVSTFYMDQHEVSNLDW
                     REYVYWMRLMFSASPKLVNRALPDTLVWRDELAYNEPYLEYYFSHVAYQEYPVVGVSW
                     EQAVDYCLWRTDRVNELRMVQAGVIELPDFKALHKDSATVEKQDSFARKQLFNVDKYL
                     KNSDYKPDKEKSKVKTVFGDARKTNMSDGILLPQYRLPTEAEWEFAAYGIKSEEGMEN
                     YDERRIFPWDGSQLRNPTKKHRGKMMANFVRGRGDYMGVAGDLNDKASITAPVSSFWP
                     NDYGLFNMAGNVNEWVMDVYRPMTSENVQEYNPFRGNMYVSPIFNDSISDAGTTRIVK
                     LDSLGRAVKAIAVSKDSISDYVKYDVRNYEDGDAKTSVDANQWKNDIDPDESTKRLYN
                     PDTDAEGMLATHISNTTRVFKGGGWKDRAYWLNPATRRYLEQTKSRSDLGFRCAMSRV
                     GSDKGNQDKK"
     gene            4427..5290
                     /gene="folP"
                     /locus_tag="PLJ82_05735"
     CDS             4427..5290
                     /gene="folP"
                     /locus_tag="PLJ82_05735"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOR41042.1"
                     /translation="MIHTNAHLSIRVKGKLLQLHTPLVMAIINVTPDSFYTKSRINLP
                     NEISTAVCSSLHQGATIIDIGAYSSRPNATDISEKEEINRLDVALSACRKEFPDSIIS
                     LDTFRASVAEFAIKEYQVDIINDISGGTLDKNMFDTIAKWNIPYVLTHIQGTPQTMQE
                     NPNYEHFIPEVLYYFSEKLDLLKQRGISDIIIDPGFGFGKTLEQNYALIAHFEVFHHF
                     QLPIMVGISRKSMIQKVIHTSAEESLNGTTALHTLLIEKGAHILRVHDVKEATEVIAL
                     TEKLKTIHHDF"
     gene            5280..6071
                     /gene="cdaA"
                     /locus_tag="PLJ82_05740"
     CDS             5280..6071
                     /gene="cdaA"
                     /locus_tag="PLJ82_05740"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOR41043.1"
                     /translation="MIFEFGLKDSIDILIVAILLFQTYKLLRGTSGMNVFIGVLIFII
                     IWFFVSFVFQMELLGSIMNKFVNVGAIVLVILFQDEIRGFMSKFGSKRKSKGLLGYIR
                     KLFFSKKAHETINYISEITDACKTLSKSKTGAIIVIQNDADLSAYAKTGEKIEAYIKA
                     RLIENIFFKNTPLHDGAMIIANSRIKAVGCILPISHTQDIPKNLGLRHRAALGISQKT
                     DATTIVVSEETGKISLSERGKLYFNISAEELLFFLTNGVDVENKK"
     gene            6117..7622
                     /locus_tag="PLJ82_05745"
     CDS             6117..7622
                     /locus_tag="PLJ82_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOR41044.1"
                     /translation="MQTSFQVVSAAEAASYINDGDVIGFSGFTPAGSTKEVAVAIADR
                     AKSFHAEGKPFKIGMYTGASTGDSLDGVLARANAVSFRTPYQSNKDLRTSINDNNVNY
                     FDMHLSMIAQELRYGFMPRPNVAIIEACDITNDGEIVLTSAVGITPTIVRLADKIIIE
                     LNHYHPKGLKGIHDIYEPLDPPYRKEIPIYKPSDRIGSPTLKIDPKKILCIVETNKPD
                     EVGGFSPIDDITKRIGENVANFLANEIKRGTIPPQFLPIQSGVGNIANAVMASLGENP
                     GIPPFEMYTEVIQDSVISLMKQGSIKFASGCSLTVTTPVLQDIYANLDFFKERLVLRP
                     QEISNSPEVSRRMGLIAINTALEADIFGNINSTHVMGTKMMNGIGGAGDFTRSAYLSI
                     YTTPSTAKGGKISAIVPMVSHVDQSEHSVKIIITEHGVADLRGKSPIQRAHEIIDNCV
                     APEYKDILNDYLKIHAKDQTPHCLCSCFGMHSEFLKSGNMLNIDWKEYENK"
     gene            7632..8168
                     /locus_tag="PLJ82_05750"
     CDS             7632..8168
                     /locus_tag="PLJ82_05750"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOR41045.1"
                     /translation="MIPFTKQAIRQLTKLKVLALSSFERIEKEITIFAQLEQLQAFKN
                     AKHILCYWALPDEVNTSMLIRTWEKEKKFYLPVVKNTELVIKPYSSSKELQQGVFAIQ
                     EPRTDSEVSLSDIDLIIVPGVAFDELGHRLGRGKGYYDRLLQHTTTTKIALAYSEQIY
                     ESIPTELHDIKMDIVVSA"
     gene            8181..>9445
                     /locus_tag="PLJ82_05755"
     CDS             8181..>9445
                     /locus_tag="PLJ82_05755"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOR41046.1"
                     /translation="MAIPTFKYQEPFPLGKDTTEYYLLTDQYVSVSNFEGKEILKVDV
                     EGLTQVANVAMRDVAFMLRPAHQQQVAAILADPEASENDKYVALTMLRNAEISAKGVL
                     PFCQDTGTATVFAKKGQHVMTNGNDEEALSFGIYKTYTEENLRYSQTIPLDMYNEINS
                     GTNLPAQIDIMSTQGDEYKFLFVAKGGGSANKTYLFQETKALLNPATLEKFLVEKMKT
                     LGTSACPPYHIAFVIGGTSADAAVKTVKLASTKYYDELPTKGNEGGQAFRDIELEQKI
                     LAAAQESGFGAQFGGKYFAHDVRIIRLPRHGASCPVAMAVSCSADRNIKAKINKKGIW
                     IEKMEDNPGKYIPESMRKAGEGDAIHIDLNRPMKEILAELSKYTVATRLSLNGTIIVG
                     RDIAHAKLKERIDSGEGLPQYIKDHPIYYA"
BASE COUNT         3075 a         1797 c         1590 g         2983 t
ORIGIN      
        1 cagctggtgt ttgtttatca gaaaaaatga gcaaggcatt atcctgaacc acctcacata
       61 ttacttttat ttcgggcatc ttgtacaaag attgtacaga agaaatttgt ggattgaaac
      121 ttttcccctc attcacggta acttgcaact gtgcatcaaa cttaccaaac aatccttcta
      181 tcactccttg aaatccgtta aaaacagaca atacacagac caatgctaca gtacctacag
      241 ctactccact tgctgaaata agcgatataa tattaatggc attgtgcgat ttttttgaaa
      301 acaaatacct cttagctatg tataaaggaa aattcacggc tattccttat tttagaagtt
      361 tatcgatatg ttcgatatag tcaagcgaat catcaaggta aaaagataaa ctaggtacag
      421 tacgtaactg aaaccgagta cgaccagcca aatgatgttt aatggtttgg cttctttctg
      481 taacaaatgc taacatatcg gcactttttt cagaaggaaa tatactcaga taaatttttg
      541 ctacgcccaa atctggactc acacgcacaa tagttggagt aatcaatacc ccttgattca
      601 tctctttcgt aatcgactgc aaaatttcac ttatttcctt ttgaatcagt cgagctattt
      661 tattttgtct ttttgtttcc atacatgtta ttactttatt ctatgcaaat atacgatatt
      721 tttctggaag ttattttttt cgtattaagg ttaatccatc acgcaagggc aataccactt
      781 tttctacacg cgtatctgct gcaatatacc tattaaatgc taatacacct gctgtttgtt
      841 tatcattggg cactaaaggt tctacaacct taccatccca caatgtgtta tctgcaaaaa
      901 tataaccgcc agatagcact ttatcaaata caacatcgta ataagcacaa taatcacgtt
      961 tatcagcatc aataaataca agatcaaatt tctcgtccat ggttggtata aggtgcaacg
     1021 aatcgccaat taccaattct atctgttctt tatatggaga tttatcaata aaagaacgag
     1081 caaaatcctc catttcatca tcaatttcta tggtaataat ttttccatca gcaggtagcc
     1141 cttctgccaa acacaaagca gaatatccgg caaatgtacc aatttcaaga atacgttttg
     1201 gttgtatcat tttagaaagc atacttaata tacgtccttg taaatgtcca gaaaccatcc
     1261 taggacgcaa caaacgcaca tgggcttctc tgtacaattc ttgcaacaac aacggctctt
     1321 gctccgaatg ttgcaatata tactcatcta attgacttga tatattcatt tttagctaca
     1381 tataatgcaa atgggtaaat tgttcttctt gcaaacattc attggcaacg tcacaaagtt
     1441 gagatgaact aattgccata atcttggctt ctatctctgt ttgttccatc aaactattaa
     1501 aatttaaaaa atgttttgcc tttgctataa tccagttttc tttattttct ccagcaataa
     1561 gcatttgggc tattaattgc tgtttagctt taaataggcg gataggagac aattgattct
     1621 cttttacctt ttttatttct ttatatataa gtcgtaatgc ttggggcaca tctgtatgat
     1681 ctgttccaaa atatactgtc cataagccag tatctgaata aggcgtgtaa tgagattcta
     1741 cggtatacac caagccactt ttttctcgca acgacatatt caatcggcta ttcattcctt
     1801 gccctcctac tatgttattt aacaaatgaa aggcaaatcg tttttcttct tgcatagaat
     1861 aagctctatt acctaatata taatgagctt gaagtgtttt tttttctaat gtaacccttg
     1921 tcggaacata gtcgataggg gtttctctag cataggttcg cttagtagca ggtatgcttg
     1981 ctatatactt gccgagtgta cgaataactt tctcgataga aaaattacca agcgtaaaaa
     2041 acaccatttc atctgttgtg tatgtacgat tgataaattc ttttaacgac tctggcgtac
     2101 atttctgtaa actttcttca gttcccaata tatttcttcc tatagcatat tgattaaata
     2161 gtaaattctc aaaatcgtct acaatcaatt cagaaggact atccatataa gaatgaatct
     2221 catctattac aacatctact tctttttgca attcatgttg agctatgatg gaattaaata
     2281 caatatctac aattacatct acagctgaat tgagatattt agtaaggaat accgaataaa
     2341 caacggtttc ttctttacta gtataagcat tcagctcacc acccacatct tctatccggt
     2401 ttattatttg cgtgctattt cttttctgcg ttcctttaaa cagcatatgt tcagtaagat
     2461 gagcatagcc ttcttcattt ggcaactcat cacgtgttcc cgtattaata ataacacctg
     2521 catatgccac ctcagaattg tcgtgcacgt gcaacaagcg tatattattt gcgaaaagat
     2581 gcgtttgtat attcatatag atagtataat ttacgtcaaa aaaggatagc ttatctagct
     2641 atccttttct atattttttc aataaacttt ctacagctta ttttttatct tgatttcctt
     2701 tgtcagaacc tacacggctc atagcacaac ggaaacctaa atcgctacga ctttttgttt
     2761 gttccaaata acgccttgtt gcaggattta accaatacgc tctatctttc caaccaccac
     2821 ctttaaatac acgagttgta ttcgagatat gagtagctaa cattccttct gcatcggtat
     2881 caggattata taaacgtttg gttgattcgt ctgggtcaat atcatttttc cattggtttg
     2941 catcaacact tgtttttgca tcaccatctt catagttacg tacatcatat ttcacgtaat
     3001 cagaaattga atccttactt actgctatgg cttttacagc tcttcctaag ctatcaagtt
     3061 ttacaattct tgttgttcct gcatcagaaa ttgaatcatt aaaaataggt gaaacataca
     3121 tgttaccacg gaaaggatta tattcttgaa cgttctcgct agtcattgga cgatatacat
     3181 ccattaccca ctcgtttacg ttacccgcca tattgaacaa accataatcg ttgggccaaa
     3241 atgaactcac tggagcagta atcgatgctt tatcattcaa atcacctgca acacccatgt
     3301 agtcaccacg accccttaca aagtttgcca tcatttttcc tctatgtttt tttgttgggt
     3361 ttcttagctg agagccatcc catgggaata tgcgtctttc gtcatagttt tccattcctt
     3421 cttctgattt gataccataa gcagcaaact cccattctgc ctcagttggc aggcggtatt
     3481 gtggcaacaa aattccatca gacatatttg ttttacgagc atctccaaac actgttttta
     3541 ctttgctctt ttctttgtct ggtttataat cagaattttt taggtattta tccacattaa
     3601 atagttgttt tctagcaaaa ctatcttgct tttcaacagt tgctgaatcc ttgtgcaagg
     3661 ctttaaagtc gggtaattca ataacgccag cttgtaccat acgtaactca tttacacggt
     3721 ctgttctcca taagcagtaa tctaccgctt gctcccagct aacaccaacc acaggatatt
     3781 cttggtaggc tacatgcgaa aaataatact ctaaataagg ttcattataa gctaactcgt
     3841 ccctccatac caatgtatca ggcaatgctc tgtttactaa ttttggtgat gcagaaaaca
     3901 ttaacctcat ccaatacaca tattctctcc aatctagatt ggaaacttcg tgctgatcca
     3961 tataaaaagt actaactgtt acacgacgag gataattatt ccactcactg tatgtatctt
     4021 cggtaacacg acccatagta aaggtacccc cttctattaa aaccatgcct ggaggagtgt
     4081 cttgctcata atcgtgaata acttgaaatc cactgtgttt tttgtcgttg tatttccaac
     4141 cagttgtatt ggaaacgttt ttatcgtttt tacaggcgaa aaaagcgata agagttaaaa
     4201 aaagaataac tttttgaaga agattgattt tcatagtttg actcaaaatt taaaattggc
     4261 ttacaaagat aacagatttt tgtgtttctt attgtgtgat ttaggtattt tttttaaata
     4321 tctgtaaaaa actgtgcaaa gatgaagata aatttttaat tttgcaaagc attcgggtgg
     4381 ctttccctat tttttcatac attttatttc tatcattacc atttttatga tacatacaaa
     4441 cgcacattta agtattcgtg tcaaaggaaa attactacaa ttacatactc ctttggtaat
     4501 ggctattatt aatgttactc ccgactcttt ctatacaaaa agccgtataa atctccctaa
     4561 tgagatatct actgcggtgt gtagctcttt acatcaagga gctacaatta ttgatatcgg
     4621 agcctattca tctcgaccaa atgccactga catttcagaa aaagaagaaa tcaatcgttt
     4681 ggacgtagcc ctttctgctt gtcgaaaaga gtttccagac agcattattt cacttgacac
     4741 atttcgtgct tcggtagcag agtttgccat taaagaatac caagtagata ttatcaatga
     4801 tatatcaggt ggaactctcg acaaaaacat gtttgacaca attgccaaat ggaatattcc
     4861 ctacgtactt acacatatac agggtacccc acaaacaatg caagaaaatc ccaattatga
     4921 acatttcatt ccagaggtgt tgtattattt ttctgaaaaa ttagatttat tgaaacaacg
     4981 aggtataagc gatattatta tagacccagg gtttggtttt ggtaaaacac ttgaacaaaa
     5041 ctatgcactc attgcacatt ttgaagtgtt tcatcacttt caattaccta tcatggtggg
     5101 tatttcgcga aaatccatga ttcaaaaagt aatacataca tcagccgaag agagtctaaa
     5161 cggaactaca gccctacata cattgttaat agagaaagga gctcatatac taagagtaca
     5221 cgatgtaaaa gaagcaacag aagtaattgc attaacagaa aaacttaaaa ccatacacca
     5281 tgatttttga atttgggtta aaagatagta ttgacatatt aattgttgcc attttattgt
     5341 ttcaaacata taaattgctt cgcggtacat caggcatgaa cgtgtttatc ggagtactta
     5401 tttttatcat catttggttt tttgtgtcgt ttgtttttca aatggaatta ttgggttcta
     5461 tcatgaataa atttgtaaat gttggagcca ttgtgttggt tattttattt caagatgaaa
     5521 taagaggttt tatgtcaaag tttggcagta aacgtaaatc aaaaggatta cttggttaca
     5581 ttcgcaaact attttttagc aaaaaagctc acgaaacaat caattatatc tctgaaataa
     5641 cagatgcatg caaaacctta tccaagtcaa aaacaggggc tatcattgtc attcaaaatg
     5701 atgctgacct atctgcatat gcaaaaacag gggaaaagat tgaagcatac attaaagcac
     5761 gattaataga aaatattttt tttaaaaaca cccccttaca tgacggtgct atgataatag
     5821 caaatagccg aatcaaagca gttgggtgca tccttcctat ttcacataca caagatatcc
     5881 caaagaacct aggtctgaga catagagctg ccctcggtat ctcgcaaaaa acagatgcta
     5941 ccaccatagt agtttctgaa gaaacgggta aaatatcgct ctcagaaaga ggaaaattat
     6001 actttaatat ttccgccgaa gaattactgt tttttttaac caacggagtt gacgttgaaa
     6061 ataaaaaata gtatctttgt tgccttaaaa acaacatact aaaatactta attaacatgc
     6121 aaactagttt tcaagtagta tctgcagcag aggctgcaag ctatatcaat gatggggatg
     6181 taatcggatt tagtggattt acccctgccg gttcaacaaa agaagtagca gtagctattg
     6241 ctgacagagc aaaaagtttt cacgcagaag gtaagccttt caaaataggc atgtatactg
     6301 gagcttctac aggcgattcg ttagatggag tattagcccg tgctaatgcg gtttctttcc
     6361 gtactcctta ccaatcgaac aaagatttac gtacgagtat taacgacaat aacgtaaatt
     6421 attttgatat gcatttgtcc atgattgctc aagaactacg ctacggattt atgcctcgtc
     6481 ccaatgttgc cattattgaa gcttgtgata taactaacga tggtgaaatt gtgcttacat
     6541 cagctgtggg tattactcca accattgttc gtttagccga caaaattatt atcgaattaa
     6601 atcattatca tccaaaagga ttaaaaggaa ttcacgacat ttatgaacct ctagatcccc
     6661 catatagaaa agaaattcct atttacaaac caagcgatcg tattggttcg cccacgttaa
     6721 aaattgaccc aaaaaagatt ttatgtatcg ttgaaacaaa caagcctgac gaggtaggtg
     6781 gtttctctcc gattgacgat ataacaaaac gtattggtga aaatgttgcc aactttttgg
     6841 cgaatgagat taaaagagga accattccac cacaattttt acccatacaa tctggcgtag
     6901 gcaatattgc gaacgcagta atggcttcat tgggtgaaaa cccaggaatt cctcctttcg
     6961 aaatgtacac agaagttatt caagattctg ttatctcgtt gatgaaacaa ggaagtataa
     7021 aatttgctag cggttgctcg cttactgtaa caacacccgt attgcaagat atttatgcca
     7081 atctagattt cttcaaagaa agattagtat tacgtccaca agaaatatca aacagtccag
     7141 aagtatctcg tcgcatggga ctaattgcca ttaatactgc attagaagct gatatttttg
     7201 gaaatatcaa cagtactcac gtaatgggta caaaaatgat gaatggtatt ggtggtgctg
     7261 gtgattttac tcgtagtgct tatttatcaa tatatactac accttctact gcaaaaggtg
     7321 gtaaaattag tgctattgtg ccgatggtgt cgcatgttga ccaaagcgaa cattctgtaa
     7381 aaataattat cactgaacac ggggtagctg atttacgtgg aaaaagccct atccaacgag
     7441 cccatgaaat aattgacaat tgtgtggcac cagaatacaa agatatttta aatgattatt
     7501 tgaaaattca tgctaaagac caaactcctc actgcttatg ctcttgtttt ggtatgcact
     7561 cagaattttt gaaatcaggc aatatgttga atattgattg gaaagaatac gaaaataaat
     7621 aactaaatgt tatgataccg tttacaaagc aagccatcag acaacttaca aaactgaaag
     7681 tgcttgcttt gtcttctttt gagcgaattg aaaaagaaat tactatattt gcacagttag
     7741 agcaattaca agcatttaaa aatgctaaac atatactgtg ctattgggct ttaccagacg
     7801 aggtaaacac atctatgcta attcgcactt gggaaaaaga aaaaaaattt tatttgcctg
     7861 tggtaaaaaa cactgaatta gtgataaaac catatagctc aagcaaagaa ttgcaacaag
     7921 gagtttttgc tattcaagaa ccaaggacag atagtgaggt aagtttgtcg gatatagact
     7981 taattatcgt tccgggtgta gcatttgatg aattagggca tcgattagga agaggcaaag
     8041 ggtattacga tagattgtta caacatacaa caacaacgaa aatagcgttg gcatatagcg
     8101 agcaaattta cgagagcata cccactgaat tacatgatat aaaaatggac attgttgtgt
     8161 ccgcataaac gatatatagt atggcaatac caacatttaa gtaccaagaa ccatttccgc
     8221 taggtaaaga tactacggaa tattatttac ttactgacca gtatgtttca gtcagcaatt
     8281 ttgaaggaaa agaaatttta aaagttgatg tagaaggttt aacgcaagtt gccaacgtgg
     8341 ctatgcgtga tgtggcattt atgctcagac ctgctcacca acaacaagta gcagcaattt
     8401 tagccgaccc cgaagccagc gaaaacgaca agtatgtggc tcttactatg ttgcgtaatg
     8461 ctgaaatatc ggcaaaagga gttttacctt tttgtcaaga tacaggtact gcaacagtat
     8521 ttgcaaaaaa aggtcaacat gtaatgacca atggcaatga tgaagaagct ttatctttcg
     8581 gtatttacaa aacatatacc gaagaaaatt tgcgttattc gcaaaccatt ccgttggata
     8641 tgtacaacga aataaattca ggaacaaatc tacctgctca aattgacatc atgagcacac
     8701 agggtgatga atataaattt ttatttgtag ccaaaggagg cggttctgca aataaaacct
     8761 atttatttca agaaaccaaa gccttgctaa accctgcaac attggaaaaa ttcctagttg
     8821 aaaaaatgaa aaccttaggc acttctgcct gtccacccta tcatatagca tttgtaatag
     8881 gtggcacttc ggctgatgcc gctgtaaaaa cggtaaaact tgcctctacc aaatattacg
     8941 acgaactgcc cacaaaaggc aacgaaggcg gacaagcatt tagagatata gaattagaac
     9001 aaaaaatact tgctgcggct caagaatcgg gctttggtgc tcaatttggt ggcaaatact
     9061 ttgctcacga tgtgcgtatc attcgtttgc ctcgccacgg agcatcttgc cctgtagcga
     9121 tggctgtatc gtgttcggct gatcgcaata taaaggctaa aattaacaaa aaaggcatct
     9181 ggatagaaaa aatggaagac aatccaggga aatatattcc tgaatcaatg cgtaaagcgg
     9241 gcgaaggtga tgcgatacac attgacctta accgtccgat gaaagaaata ttggctgaat
     9301 tatcaaaata cactgtggct accagattat cactcaatgg caccattatt gtaggtagag
     9361 atattgctca tgcaaaatta aaagaacgca ttgatagcgg agaaggttta ccacaataca
     9421 ttaaagatca tcctatttac tatgc
//
LOCUS       DAOSRP010000074         9433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7007_length_9433_cov_6.037535, whole genome shotgun sequence.
ACCESSION   DAOSRP010000074 DAOSRP010000000
VERSION     DAOSRP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7007_length_9433_cov_6.037535"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PLJ82_05760"
     CDS             <1..1034
                     /locus_tag="PLJ82_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR41047.1"
                     /translation="IYDQTINRLTVIANESYDDFAKALQKEIEEDCGVKFTGRIKNSK
                     DRTQIKYRKGFEADPRFLEIWEKIKTKTSYRVDYSTNDLITLAAKAIKEIPEIKAPSI
                     RSTKVSVTMTDEGVGTSYVGDKIESYGGYSWKIPDVLGYIQSKTELTRSTIQEIISKS
                     GRINDILINPQLFLDLASQAIKRTLYDLMIDGIKYHKIGNTEYYTLELFDDKDFEVYL
                     NDFAFKVSDNSKTIYEEYIPLDSGVESRFAQDCETSEQVKFYFKLPNWFKIPTPIGNY
                     NPDWAIVFEGDRKIYFVAETKDTGTPQVDLSKLGGDEQLKIKCGKAHFNVFENIEYKV
                     VNKIGQLIE"
     gene            complement(1298..1681)
                     /locus_tag="PLJ82_05765"
     CDS             complement(1298..1681)
                     /locus_tag="PLJ82_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR41048.1"
                     /translation="MPLLAIGVVIAGFLLGSTHDDVSIPGIIPNEWVAWAVGGNSILS
                     NFLASIVGAFMYFATLTEVPIVQGLLASGMGKGPALALLLAGPSLSLPNMLVIRGVMG
                     TQKTIVYVAIVVVLSTIAGVVFGSL"
     gene            complement(1900..2616)
                     /locus_tag="PLJ82_05770"
     CDS             complement(1900..2616)
                     /locus_tag="PLJ82_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR41049.1"
                     /translation="MELKTELKILLAIVVVFLGIYFLPLESVVFATAIDATLDLSRWY
                     AREHVILCLLPAFLIAGVIAVFVSQGSVIKYFGAKAKRWVAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAVAFLYSGPAINILAIILTARILGFEMGVARVIGAVVFSVV
                     IGLIMAFIYRKEEKVKAQEQLSFPDVPEKRPLWQTSLHFLPWYLSWYLPIGGDQPKAI
                     QRVFGFIYGNINGILQGYLV"
     gene            complement(2672..3385)
                     /locus_tag="PLJ82_05775"
     CDS             complement(2672..3385)
                     /locus_tag="PLJ82_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOR41050.1"
                     /translation="MEFLQTIVSQSEIPILTAFVLGLMTAISPCPLATNITAIGFISK
                     DMQQKRKVFINGLVYTLGRAVSYTVLGIMLIVLFQQGVSLFKVQRFISLYGMYLLVPF
                     LLVMGVFMLDFIKISLPGFSRMTLKMEEKASSGSAWSSFLMGIVFALAFCPYSGVLFF
                     GALIPLSVSVSAGYALPFVFAIATGLPVVIFAWIFAFSISSVSLFYNKIKVFEKWFRR
                     VVGVLFIVVGLYFAAILFF"
     gene            complement(3385..3780)
                     /locus_tag="PLJ82_05780"
     CDS             complement(3385..3780)
                     /locus_tag="PLJ82_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41051.1"
                     /translation="MKKTLILAVLALMSVTVVVAQKAEVLYFKADMPCCKARACNNLE
                     ADIKAVVEKNYPKGDVVFKQVRIADEANKALVEKHQAKAQTIVIVGKKKKKKQQVIDV
                     SEVVRGCVRSGDKTELETKLVEAINKAIK"
     gene            complement(3785..4198)
                     /locus_tag="PLJ82_05785"
     CDS             complement(3785..4198)
                     /locus_tag="PLJ82_05785"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOR41052.1"
                     /translation="MKQKMSVLFVVFLLFVSCAKTESTEQVAHENDVAKVTVYYFHGK
                     QRCPTCVSIQQTAQSAVDTYFANNEDVRFVEVDFSVKKNDALAEKYEVAFSSLIIATA
                     DAYSDITDQAFALATSNPEQLKTIINQEVTKYLNQ"
     gene            complement(4211..4447)
                     /locus_tag="PLJ82_05790"
     CDS             complement(4211..4447)
                     /locus_tag="PLJ82_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR41053.1"
                     /translation="MEIKVLGPGCPKCKSLEKLVQETVQEMQLDATITKVEDIMEIMQ
                     YNIIATPALVINGKVVLKGVLPSKVQLQIILSTN"
     gene            complement(4463..4774)
                     /locus_tag="PLJ82_05795"
     CDS             complement(4463..4774)
                     /locus_tag="PLJ82_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOR41054.1"
                     /translation="MAKKNFTEEQEKLARYNKALGHPVRVFIMEYLVKNASKCCYSGD
                     MIENLPIARSTLSEHLKELKKAGFIQGEINPPYIKYCINKENWEEAKQMVMDFYSINN
                     D"
     gene            complement(4911..5855)
                     /gene="mdh"
                     /locus_tag="PLJ82_05800"
     CDS             complement(4911..5855)
                     /gene="mdh"
                     /locus_tag="PLJ82_05800"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOR41055.1"
                     /translation="MKSKVTVVGAGNVGATCANVLAINEVASEVVLLDVKEGVAEGKA
                     MDIMQTAQLMGFDTVVKGCTNDYAQTANSDVVVITSGVPRKPGMTREDLIGINAGIVK
                     SVAESILAYSPNAILIIVSNPMDTMACLAMKTLGLPSNRIIGMGGALDSSRFTYYLSQ
                     ALQCNANDVQGMVIGGHGDTTMVPLTSLATYKGVPVSKLLSKEKMEEVAKATMVGGAT
                     LTGLLGTSAWMAPGAASAIVVEAILRDQKKIIPCSVVLNGEYGLNDIMIGVPVILGKN
                     GVEKIVELELSEEEKEKFAASAVAVRKTNEILKEMNLV"
     gene            6088..8895
                     /locus_tag="PLJ82_05805"
     CDS             6088..8895
                     /locus_tag="PLJ82_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR41056.1"
                     /translation="MQFKRVLTCIIGLSIMLIFSQCSTKNNTWLSRNFQSFSTRYNVY
                     FNANESYKKGIEKIASGHKDSYTEILPIFPISVHTNASFATNEMAISIEKSEKAIKNH
                     SIKRKPKRNPSKLQDPSYQAFLNQSEYNPMVEQSWLLIGKSQFHQADFMAANATFLYI
                     IRNYIHNQELVTAATIWLARSYGEVGWFYEAEDLMNKIDEGGFSPTTNVLFVYVKADL
                     LLKQKRYQEAVPFLESAYNAEKKQQQQARLGFILGQIYEKEQQYDKAFTYYNYVLKKN
                     PPYEMAFNANLKKTQSYQGKDYEKMIKDVTKLASNERNYEYLDQLYTAIAKLYQRNGK
                     TTKAIEYYQLAIEKSTRNGMDKAEALLSLGQLYINNKEYVKAQPLINEAVPILPSSYS
                     NYRSWRTISENLNELALHHNRIVLEDSLQKLAQLPEIERIKRIQEVIKVEQEAQEKLK
                     EKIDKEAKQQEERERNAFSTLPSLALGEIGDKSWYFYNPTLIGRGKIEFQRTWGNRIL
                     EDNWRRKNKTAVLNDRQETIDEVVQKIEPSSTTENVTQQPDSISPTDKQIAKYLQQIP
                     LSVAQKENSDNAIMESLYNLVYIYKEKVPDYELAQQTYAELIERFPSSSFIAEATFNM
                     FQLHSKEQNLLEAERSKQYILANFPTSTYAVFLQNPEELNTLTQLRKEEEKLYAETYE
                     AFLKNDYAIVALNLEKAQKEFPFSKLTPKFRFLEALSTSKKKGKDAFKTSLELIVKDY
                     PDSEITPMAKDMIALMKQGQEPQSSLSHSNLLAERTSVVTEQVFAENIAKAGFVYEPQ
                     DNHLFVLIVNTDSITRNTLIFNIASYNFTRFLIKELDIQVKELDDVSVAIAISGLDNL
                     SEAIWYQKTLLADSTVTKQLQKTDYKGFVISEYNFSSVTNTESLERYFEFYRTNNLQI
                     TEPNTIKQLEQESGFVKGNAE"
     gene            9061..9384
                     /locus_tag="PLJ82_05810"
     CDS             9061..9384
                     /locus_tag="PLJ82_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOR41057.1"
                     /translation="MTKHIFSPKRYLSSFLFVFFFFLSIVISFAQSITGTVIDKKTNE
                     TLAEAAIAVNGQKVYTDLDGNFTLANLGGSKCELTVSMISYETQTIEMDLAKNKDVAI
                     VLKKR"
BASE COUNT         3337 a         1860 c         1533 g         2703 t
ORIGIN      
        1 gaatttacga ccaaaccata aaccgattaa ctgttattgc aaacgaatcg tatgatgatt
       61 ttgcaaaagc acttcaaaaa gaaattgaag aagattgcgg ggttaaattt actggacgca
      121 ttaagaactc aaaagaccgt acacaaatta aataccgcaa aggttttgaa gccgacccac
      181 gttttcttga aatttgggaa aagattaaaa ccaaaacaag ctatcgagtt gattattcaa
      241 caaacgactt aattacactt gctgcaaaag cgataaaaga aatacctgaa attaaagcac
      301 catcaatacg ctcaacaaaa gtttcggtaa caatgaccga tgaaggtgtt ggaacttcgt
      361 atgttggcga caaaatagaa tcgtatggcg gttattcatg gaaaataccc gatgttttag
      421 gatacattca aagcaaaacc gaattaacac gttcaacaat tcaagaaata ataagcaaat
      481 cgggaaggat taatgatatc ctaattaacc ctcaattgtt ccttgatttg gcttcacaag
      541 ccattaaacg cactctgtac gatttaatga ttgatggtat aaagtaccat aaaattggca
      601 acacagaata ttacacactt gaattatttg atgataaaga ttttgaagtc tatttgaacg
      661 attttgcttt taaagtttca gacaattcaa aaacaattta tgaagaatac attcctttag
      721 attcaggcgt agaaagcaga tttgcacaag attgtgaaac aagcgaacaa gttaaattct
      781 attttaaact tccaaattgg tttaagattc caactccgat tggaaattat aatcccgact
      841 gggcaatagt tttcgagggc gacaggaaaa tttattttgt agcagaaacc aaagacacag
      901 gaacaccaca agttgattta tcaaaattag gcggagacga acaactgaaa ataaaatgcg
      961 gtaaagcaca tttcaacgta tttgaaaata ttgaatataa agtagttaat aaaataggac
     1021 aattgattga gtaatgtcaa gagggcttac catataacgt aaccaaatat aggtattttt
     1081 atggtatcta tttttatttt ttattcagta tacttagcct taggatacaa ttacaaaatc
     1141 tgaactgagg gaagattagc gtattattgt cgttacaaac gacaatatat aaactcctcg
     1201 ttacaaacga ggagtaggaa gattaattga atttcaggca atagagttga aagaaaacaa
     1261 atatgcaact ttagaacttt atgaacttgt aaactattta caacgaccca aaaacaactc
     1321 ctgcaatagt tgataataca accacaatag ccacatacac aatagttttt tgcgtgccca
     1381 ttacacctct aattaccaac atattgggca acgacaacga tggacctgcg agcaacaatg
     1441 ccaaggcagg acctttgccc atccccgaag ctaaaagccc ttgtacgatg ggaacttcgg
     1501 taagtgtagc aaaatacata aacgctccca caatagaggc tagaaagttt gataaaatgg
     1561 aatttccgcc tactgcccac gctacccatt cattgggaat aatgcctgga atggatacat
     1621 catcgtgtgt agagcctaac aaaaatcctg ctatcactac cccaattgcc aacaacggca
     1681 taatttgctt ggtaaaaccc caactagata aaatccactc cttgttgtct gtatcttccg
     1741 atttatcaag caaagcaata gaacttactg ctactattgc aaccaccatt accaccaagg
     1801 gattgccaat aaaaaaagca gccaatacag taactacagc tcctaacaac acttgccacg
     1861 atgggagttt taaaatacgt atcatggaat aaacgagcac taaaccaaat acccctgtaa
     1921 tatgccattt atattgccat agataaaacc aaaaactcgt tgtatcgcct tcggctggtc
     1981 gcccccaatt ggcaaatacc aagataaata ccaaggtaaa aaatgcagcg atgtttgcca
     2041 taaaggtctt ttttcgggta catcaggaaa cgataattgc tcttgtgctt ttactttttc
     2101 ttctttgcgg tagataaatg ccattatcaa tccgataacc acactaaaca ctactgctcc
     2161 aattacacgt gcaacgccca tttcgaaacc caaaatacgt gccgtaagaa taatggctaa
     2221 gatattgatt gcaggacctg aatacaaaaa tgctactgct ggacctaagc ctgctcctcg
     2281 cttatgaatg ctagaaaata agggtaatat agtacacgag caaacggcta aaatagtgcc
     2341 cgatacggca gctactgtat atgctaccca gcgtttggct ttggctccaa aatactttat
     2401 taccgaacct tggcttacaa ataccgcaat aacacccgca ataagaaacg caggcaacaa
     2461 acacaaaata acatgctcgc gtgcgtacca tcgggataaa tcgagcgtag catcaatagc
     2521 cgtagcaaaa acaacacttt cgagcggtaa aaaataaatg cctaaaaata ctacgacaat
     2581 ggctagtagt attttcaatt ccgttttgag ttccatagtt ttataattat ttcgttaaaa
     2641 cacgaaatga atacgtaaaa attttttata gttagaaaaa taaaatagca gcaaaataaa
     2701 gtccaaccac gataaaaagt acaccaacta cacgtctaaa ccatttttcg aatactttta
     2761 ttttgttgta aaacaagctt acgctggata tactaaaagc aaatatccag gcaaaaataa
     2821 ccacaggcaa accagtagct atagcaaaca caaaaggcaa ggcataccct gctgatactg
     2881 aaacagacaa cggaataagt gccccaaaaa ataacacacc actatacgga caaaaagcca
     2941 gagcaaatac aattcccatc aaaaaggaac tccatgcaga acctgacgat gctttttctt
     3001 ccatttttaa tgtcattcga ctaaatcccg gtagcgatat tttaataaaa tcgagcataa
     3061 aaacacccat taccaataga aaaggaacaa gtaaatacat gccatacaaa ctaataaaac
     3121 gctgaacttt aaaaaggctt acaccctgct gaaacaacac aatcagcata atacccaata
     3181 cagtataaga cacagcccta cccaatgtat atacaagtcc attgataaat accttgcgtt
     3241 tttgctgcat gtctttgctt ataaacccga tagccgtgat attagtagcc aaagggcaag
     3301 ggctgatggc agtcatcaac cctagcacaa aggctgttaa aatcggaatt tcagattgtg
     3361 aaacaatggt ttgtaaaaac tccactactt aatggcttta ttgattgctt ctactaattt
     3421 ggtttcgagt tcagttttat caccactgcg tacgcaacca cgcactactt ctgatacgtc
     3481 aattacttgt tgttttttct ttttcttttt gcctacaata acgatggttt gagctttggc
     3541 ttggtgtttt tctaccaagg ctttgtttgc ctcatcagca atacgaactt gcttaaatac
     3601 tacatctcct ttgggatagt ttttttctac cacagctttt atgtcggctt ctaagttatt
     3661 acaagcacga gctttgcaac aaggcatatc ggctttaaaa taaagaactt cggctttttg
     3721 agctacaact actgtaactg acatcagtgc caatacggct aaaataagtg tttttttcat
     3781 acttttattg atttaaatac ttggtaactt cttggtttat tattgttttt aattgttctg
     3841 gattactagt ggcgagagca aaagcttggt cggtaatatc gctgtaagca tctgctgtag
     3901 caatgattaa tgagctaaat gctacttcgt atttttcggc taaagcatca ttttttttga
     3961 cagagaaatc tacttctaca aaacgtacat cttcgttgtt ggcaaaatac gtatcgacag
     4021 ccgattgggc agtttgctga atggaaacgc aagtaggaca acgttgctta ccatgaaaat
     4081 aatatacagt tactttggct acatcgtttt catgggctac ttgctctgta gattcggttt
     4141 tggcacacga aacaaacaag agaaaaacaa caaataggac actcattttc tgtttcatag
     4201 gaaggcctgt ttagttcgtt gacaaaatta tttgtaattg cactttcgag ggcaatactc
     4261 ctttcagcac aactttaccg ttaatcacta aagcaggagt ggcaataata ttgtattgca
     4321 taatttccat aatatcttct actttggtaa tggtggcatc aagttgcatt tcttgcactg
     4381 tttcttgcac taatttttct aaacttttac atttcggaca ccctggtcca agtactttaa
     4441 tttccatact cattatctta aattagtcgt tattgattga ataaaaatcc atcaccatct
     4501 gtttggcttc ttcccagttt tctttgttaa tgcaatactt aatatacggc gggttgattt
     4561 ccccttgtat aaaacctgct tttttcaatt cttttaagtg ttcggacaag gtagagcgtg
     4621 caatgggtaa attctctatc atatcgccac tatagcaaca ttttgaggca ttcttaacta
     4681 agtactccat aataaataca cgtacaggat gacccaatgc cttgttgtaa cgagctaatt
     4741 tttcttgttc ttctgtaaag tttttctttg ccataattcg tatttcgtaa aaagacgaaa
     4801 caaagataaa taagtttacg gtacaaggca caaaaaaact gctaaattaa ttttatttag
     4861 cagttttaac gtttgaatag ggaagataaa tagtggatag gattgaaatc ctataccaaa
     4921 ttcatttcct ttaaaatttc gttggttttg cgaacagcta ctgcactggc agcaaatttt
     4981 tctttttctt cttcgcttaa ttcaagttca acaatttttt caaccccatt tttccctaag
     5041 attacaggca caccaatcat aatatcgttt aagccgtatt caccatttaa aacaaccgaa
     5101 caaggaataa ttttcttttg gtcacgtaat atagcttcca ccacaatagc cgatgctgca
     5161 cctggagcca tccatgccga cgtgcctaaa agtccagtta acgtagcacc tcctaccatg
     5221 gtagcttttg ccacttcttc cattttttct ttgcttagta atttgcttac tggaacgcct
     5281 ttatacgtag ctaaactggt aagtggcacc attgtagtat cgccatgtcc tccaattacc
     5341 atcccttgca catcgttggc attacattgc aatgcttggc tcaaataata cgtaaaacgt
     5401 gaactatcta atgctcctcc cataccaata attctgtttg aaggtaatcc taaagttttc
     5461 atagccaaac atgccatcgt atccattggg ttggaaacga taattaaaat tgcattaggc
     5521 gaatatgcca aaatactttc tgccacactt tttactatac cagcgttaat acctatcaaa
     5581 tcttctcttg tcatacctgg tttacgtggt actcccgacg taattaccac aacatccgaa
     5641 ttggcagttt gagcataatc gttggtacaa ccttttacta ccgtgtcaaa tcccattagt
     5701 tgggctgttt gcataatatc catggctttc ccttcagcca caccctcttt cacatctagc
     5761 aaaacgactt cgcttgctac ttcattaatt gctaatacat tagcacaggt tgcccctaca
     5821 tttcctgctc ctacaacggt tactttcgac ttcattattt gtatttttaa aaagtggtga
     5881 ttttactgcc cacaaaatta caattttttc tttcatatca aagaaagaag agaataaatc
     5941 agcacttcct taacaaaaaa ataacatttt ttacaccctg ttttcaacaa taaaagacag
     6001 gaacacttaa atttacaaaa aacgaagcta tttttcatag catactagca taatttgtgt
     6061 acttttgtac cgaaaatggg atatactatg caatttaaac gagtgcttac ctgtataatc
     6121 gggttgtcga ttatgcttat ttttagccag tgttcaacaa aaaacaacac atggttgtct
     6181 cgtaattttc agtctttttc cacacgctat aatgtttatt tcaatgccaa tgaaagctat
     6241 aaaaaaggaa tagaaaaaat tgcttcggga cacaaagatt cctacaccga aatattgccc
     6301 atttttccaa tcagcgtgca tacaaatgcc tcttttgcta ccaacgaaat ggctatcagc
     6361 atagaaaaaa gcgaaaaagc cataaaaaac cactccataa aacgcaagcc aaaacgcaat
     6421 ccatccaaat tacaagaccc atcctatcag gcttttctga atcaatcaga atacaatcca
     6481 atggtggaac aatcttggct tttaattgga aaatcacaat tccatcaagc tgattttatg
     6541 gcagctaatg ccactttttt gtatattatc cgtaattata ttcataacca agaattggtt
     6601 acagcagcaa caatatggtt ggctcgtagc tatggtgaag tcggttggtt ttatgaggca
     6661 gaagacctga tgaataagat tgacgaagga gggttcagtc cgacaacaaa tgtattgttt
     6721 gtgtatgtaa aagcggattt actactcaaa caaaaacgat accaagaagc cgtccctttt
     6781 ttagaatcag cttataatgc tgaaaaaaaa caacagcaac aagctcgctt aggatttatc
     6841 ttgggacaaa tttacgaaaa agaacagcaa tatgacaaag catttacgta ttacaactac
     6901 gtactgaaaa aaaatcctcc gtatgaaatg gcgtttaatg ccaacttgaa gaaaactcaa
     6961 tcgtaccaag gaaaagatta cgaaaaaatg attaaagatg taactaaatt agcatccaat
     7021 gaacgtaatt atgaatactt agatcagcta tatacggcaa tagcaaaact ttatcaacga
     7081 aacggaaaaa cgactaaggc aatagaatac taccaacttg caatagaaaa aagcacacgc
     7141 aatggcatgg ataaagcaga agctcttttg tctcttggac agctatacat caacaacaaa
     7201 gaatacgtaa aagcacaacc tttgataaat gaggctgtac ctatcttgcc ttcctcatat
     7261 tctaactatc gttcgtggcg tacaatatcc gaaaatctga acgaacttgc tttacaccac
     7321 aatcgaatag tgctagaaga cagcctccaa aaattggcac aattacctga aattgaacgc
     7381 ataaaacgga tacaagaagt aataaaagta gaacaagaag ctcaagaaaa attaaaagaa
     7441 aagattgaca aagaggcaaa acaacaagaa gaacgcgaac gcaatgcatt ttctactctt
     7501 ccctccctcg ctttagggga aattggagat aaatcatggt atttttacaa ccccacatta
     7561 atcggaagag gaaaaataga atttcaacgt acctggggaa atagaatact agaggataac
     7621 tggcgaagaa aaaacaaaac agcagtatta aatgataggc aagaaacaat agatgaagtt
     7681 gttcaaaaga tagaaccctc gtcaacaaca gaaaatgtaa cacaacaacc agactccata
     7741 tcacctacag acaaacaaat agcgaagtat ttacaacaga taccgctttc agtggcacaa
     7801 aaagaaaatt ctgataatgc cattatggaa agtttataca atcttgtgta catatataaa
     7861 gaaaaagtac ctgattatga attggctcaa caaacatatg ctgaattaat cgaacgattt
     7921 ccttcgtcct ctttcattgc cgaagcaaca tttaatatgt ttcaattaca tagtaaagaa
     7981 caaaacttgt tagaggcaga aagaagcaaa caatatattt tagcaaattt cccaacttca
     8041 acctatgctg tttttttgca aaatccagag gagctcaaca ccctaacaca attacgaaaa
     8101 gaagaagaaa aactctatgc tgaaacatac gaagcatttc tgaagaatga ttatgctata
     8161 gttgccttaa acttggaaaa agcacaaaaa gaatttcctt tttctaaact tacgccaaag
     8221 tttcgctttt tagaggcatt aagcactagt aaaaagaaag ggaaagatgc ttttaaaaca
     8281 agccttgaac ttatcgtaaa ggactatcct gacagtgaaa ttacaccaat ggcaaaagat
     8341 atgatagccc taatgaagca aggacaagaa ccccaatcct ctctttcgca cagcaacttg
     8401 ctcgccgaac gcactagcgt agtaacggaa caggtatttg cagaaaacat tgccaaagca
     8461 ggttttgtat acgaaccaca ggacaaccat ttatttgtat taatagttaa cacagattca
     8521 attacccgaa atactcttat ttttaatata gcatcgtata actttactcg ttttttaatt
     8581 aaagaattgg atattcaagt aaaagaatta gacgatgtaa gtgtcgcaat tgccattagt
     8641 ggattagata atttatctga agctatatgg taccaaaaaa cactgttagc agatagtact
     8701 gttacaaaac aattacaaaa aaccgattac aaaggttttg taatttctga atataatttt
     8761 tcttctgtta caaatacaga atcattggaa cgttattttg aattttatcg taccaataac
     8821 ctacaaatta cagagcctaa taccatcaag caattagagc aagaatcggg gtttgtaaaa
     8881 ggtaacgccg aataacagtt cagcaaattc gacatgtaaa agagctggtt tttccccgaa
     8941 caaaatcgtc acaagtccgt aatcattccg taacaagaac gcaacacccc gtttttacct
     9001 ttgcggttgt aaaatagatt cgtttatcaa aaacgaccta tacctaaact taactgcaat
     9061 atgacaaaac acattttttc gccgaaacga tacctatcat cattcctatt tgtttttttc
     9121 ttttttttat ccatagtcat aagttttgct caaagtatta ctggaactgt tattgataaa
     9181 aaaacaaacg aaactcttgc agaagcagca attgcggtaa atggtcaaaa agtatacacc
     9241 gacttagatg gcaattttac cctagcaaac ctaggtggta gcaaatgcga acttacagtg
     9301 agtatgattt cgtacgaaac acaaaccatc gaaatggatt tggctaaaaa taaagatgta
     9361 gctattgtat tgaaaaaaag ataaagggta ttgattgata aaaggataaa gagcgatgaa
     9421 aaaatttttt cat
//
LOCUS       DAOSRP010000075         9424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7017_length_9424_cov_5.322873, whole genome shotgun sequence.
ACCESSION   DAOSRP010000075 DAOSRP010000000
VERSION     DAOSRP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_9424_cov_5.322873"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLJ82_05815"
     CDS             complement(<1..1134)
                     /locus_tag="PLJ82_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOR41058.1"
                     /translation="MRLSDLQTGEKAIIVKVLGHGNFRKRIIEMGFIKGKKVEVLLNA
                     PLKDPIKYKVMDYEVSLRRNEAELIRVISEKEALASSTTSNYFGTIEASSLDAIAKKT
                     GHIINVALVGNPNSGKTSFFNIASGSHERVGNYSGITVDAKEGHFTLGDYKFRIFDLP
                     GTYSLAAYSPEERYVRKHIFEETPDVIINIVSASNLERNLYLTTQLIDMDVPMVIALN
                     MYDELEKHNHTLNYLQLGKMIGVPIVPTVAVKGYNNEKNGIKHVFEKVIEVYEGNEPT
                     VRHIHINHGGDLQLAIDKLNDLIKEDAHFKSHIAARFFAVKLLEKDKEAEQYIHSLAN
                     QKDILGYTEKAIPFLENELKDDTESAIINAKYGFIAGALAETFT"
     gene            complement(1227..1844)
                     /locus_tag="PLJ82_05820"
     CDS             complement(1227..1844)
                     /locus_tag="PLJ82_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOR41059.1"
                     /translation="MTTQTNQRIIIIEPSTIVFAGLQSIIERMHIAESIYHVTELDTI
                     TQKLAQLRADIIIVNTAVFGFHKNLSIKSIFKDCTHASIIAFVSQYSDSQFLKQFDDI
                     IDIYDDAQTISAKIRHIIEQDTIDITETDTTELSEREQEVLIAVVKGLINKEIASTLN
                     ISIHTVMSHRKNITRKTGIKSISGLTVYALFNNLIKQEDVQQPHH"
     gene            complement(1868..2560)
                     /locus_tag="PLJ82_05825"
     CDS             complement(1868..2560)
                     /locus_tag="PLJ82_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOR41060.1"
                     /translation="MKQIHFTPQMKLASLILHNFKLLPVITRFGIPLGFGEKTVAEVC
                     SHYAVPTDFFLLVCTIYTQDDYMPTPSEIQTIDIHTLLSYLSNSHQYYLTERIVEIEQ
                     SIHAMNQSCSNKHYSIIETFFDGYKKEVIKHLEYEDSSVFPYVYKLIAGEKTPGFGIQ
                     QFEKNHTNIEEKLGDLKNIIIKYVPNDCPSRERNKLLYDIFLFEEDLNRHTLIEDKVL
                     IPYVESIEKSQN"
     gene            complement(2804..4249)
                     /gene="pyk"
                     /locus_tag="PLJ82_05830"
     CDS             complement(2804..4249)
                     /gene="pyk"
                     /locus_tag="PLJ82_05830"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOR41061.1"
                     /translation="MSKATKIIATISDKRCDVDFLRQLYNEGMNVVRLNSAHLDLEGF
                     LRIINNTREVSNQIAILMDTKGPEVRTTIVQNNSIDIKTGDKIKISGNPDKISTHEHI
                     YVNYPFFVRDLNVNDDILFDDGEIDLKVIEKTTDYLLCEAQNDGMLGSRKSVNVPGVR
                     INLPSLTERDKQNILIAIENDIDFIAHSFVRNAQDIKDIQAILDQHNSPIKIISKIEN
                     QEGVDNINEILEQTYGVMIARGDLGIEVAQEKIPGIQRRLIRKCVQAKKPVIVATQML
                     HTMIHNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVEAVRTMATIAKEAEKTK
                     MAENDIPIEISTNDTTSFLCNAAVSACSNLGSKAIFTDTYSGLTARYLAAYRGQNPIF
                     AICYHERSTRLLALSYGVFPFFQDSGNDNTQQYIIKGLQFLLNEKWININDTVCYLSG
                     SFGVGFGTTFLEINTVKKIFESREKYILPNF"
     gene            complement(4501..5763)
                     /locus_tag="PLJ82_05835"
     CDS             complement(4501..5763)
                     /locus_tag="PLJ82_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOR41062.1"
                     /translation="MKLNKLKRSLDLKDAVAVGLGAIIGAGIFVVTGVAAGVSGPSFI
                     IGLLIAGTIATFNGLSSSQLAAVYPQSGGTYEYGYRLLTPSLGFSAGWMFVLSKLSAG
                     GIVAIGFGSYFHQLIPYFSPIEYSIAAIVLLTFANLWGIQKVGTINIAIVLITLLSLL
                     YFVVGGIPSVDTTNFIPFAPFGWKGIAESAAILFFAFTGYARIATLAEEVRNPRKTIP
                     KAVIITIAVAILLYVGVSIVATGVLGAPSMASTSSPLQLAADRIQAPGMSVVITLGAS
                     TAMLGVLLSQIVGISRMLLAMGRRNDLPHFFESIHKKTAVPHIAILFTSAIILLITLF
                     GSFPFVVRTATFTILLYYTIANAAAIRQPRIEQIYGSIIPWLGLIGCIVMAFSLPCMV
                     IVSGFGLLLIGFIARYIYQYSSKIKNGE"
     gene            6080..7459
                     /locus_tag="PLJ82_05840"
     CDS             6080..7459
                     /locus_tag="PLJ82_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR41063.1"
                     /translation="MIQKHSPLSLGTESIGKLLAQYAIPAIVAMTASSLYNITDSVFI
                     GHGVGALAISGLALTFPVMNLAAAFGSLVGAGASALMSMRLGQKDYDTANKVLGNVFV
                     LNIIMGLAFTGIMLVFLKPILYFFGASEATLPYAYDYLQVILYGNVVTHMYMGLNALL
                     RSSGRPQKAMYATICSVILNLGLTPLFIYVFGWGIKGSAWATVLSQLLMLIWQITIFS
                     NKKNFIHLKKGVFRLEKKIVLDSFAIGLSPFFMNAASSVIIIVINQALSRHGGDLAVG
                     AYGIVNRVVFLFAMVVLGLNQGMQPIAGYNFGAQHYDRVDKVLRTTILYATGIMIFGF
                     IIGQFFPFSVASVFTTDVQLTEHAVKGLRIVMIFFPIIGFQMVTGNFFQSIGQAKKAI
                     FLSLTRQMIFLLPCLLIFPSIWGVKGVWFSLPTADLASSLVAGWLLSKQLKQFKRSKQ
                     TKSCDEVGI"
     gene            7486..8094
                     /locus_tag="PLJ82_05845"
     CDS             7486..8094
                     /locus_tag="PLJ82_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOR41064.1"
                     /translation="MNNNFIITIGRELGSGGRIIGAQLAKKLNSAFYDKELILRASKE
                     SGLSSSFMEQVDEHFSPIQTGGLLGDFFQQFYFSSESCFQIQSDVIREIAEHESAVIV
                     GRGADYVLREHPRCLNIFVTANMSDRIQRVAESHTISEDEARILIEKADKKRASFYNY
                     FTSKEWGRAKSYDLCVNSSVFGLEKTTDYIFQLVKDRFSPIP"
     gene            complement(8258..9139)
                     /locus_tag="PLJ82_05850"
     CDS             complement(8258..9139)
                     /locus_tag="PLJ82_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOR41065.1"
                     /translation="MSKAKISIRFFDDREVRALWDEENAKWRFSVLDIVAVLTDQNDY
                     TKTRNYWKYLKAKLKKENSELVSATTQLKLLANDGKRYLTDMLDYDGIIALGKEFPGK
                     KANRFIEWFTYSDESIDGKSKTKAYALFESTFINSIEVGTAKGLQQIHAYLFGGLYDF
                     AGQIRQKNISKGGFQFAVSQFLGETLKLIEEMPETTFEEIVDKYVEMNMAHPFMEGNG
                     RSTRIWLDLILKKRLNKCVDWSKIGKKVYMNAMIQSPLNSSALKKILESSLTDKVNDR
                     EMFMKGIDYSYYYEEND"
BASE COUNT         2695 a         1588 c         1851 g         3290 t
ORIGIN      
        1 ggtaaaagtt tcggcaagag ctcccgctat gaacccatat tttgcattga taatagccga
       61 ttcggtgtcg tcttttaact cattttccag aaaaggaatt gctttttcag tatatcccaa
      121 aatgtctttt tggtttgcca gcgagtgaat atattgttcg gcttctttgt ctttttcgag
      181 aagtttaaca gcaaaaaaac gagcggcaat atgactttta aaatgagcat cttctttaat
      241 caggtcgttg agtttgtcaa tagccaattg taaatcgcca ccatggttga tatgtatatg
      301 gcgtacggta ggttcattcc cttcgtaaac ctctattact ttttcgaata cgtgtttgat
      361 tccgtttttt tcgttgttgt atccttttac ggcaactgtg ggcacaatgg gtacaccaat
      421 cattttacct agttgaaggt aattgagcgt atggttgtgt ttttccagtt catcgtacat
      481 attgagtgct attaccatag gcacgtccat gtcgatgagt tgtgttgtaa ggtacaaatt
      541 gcgttcgagg ttagaggctg atacaatatt gatgattaca tcgggtgttt cttcaaaaat
      601 atgtttccgc acatagcgtt cttcgggcga gtaggcagct aaggagtatg tgccaggaag
      661 gtcgaaaata cgaaatttat aatcgccaag tgtaaaatgt ccttctttgg catctaccgt
      721 aatgccactg tagttgccaa cacgctcgtg gctacctgag gcaatgttga aaaaggaagt
      781 tttgccagag tttggattcc ctactaaagc tacgttgata atatgacctg tttttttagc
      841 aatggcgtct aacgaagaag cttctattgt accaaaatag ttggatgtag tagagctcgc
      901 taaggcttcc ttttcgctga taacacgtat taattcagct tcgttacgac gtagagatac
      961 ctcgtaatcc attaccttgt atttaatggg gtcttttaaa ggtgcattga gtagtacttc
     1021 tacttttttg cccttgataa agcccatttc gataatgcgt ttgcgaaaat tgccatgtcc
     1081 tagaaccttt actataatgg ctttttctcc tgtttgcaaa tcggatagac gcattgattg
     1141 tagtttttag cttgcaaagg taagtatttt atttttgctt tgcaatgact ataagtagtg
     1201 aaaaatagcc tgttgactca ctataactag tgatgaggct gttgtacgtc ttcttgctta
     1261 attaggttgt tgaacagggc atatacagta agtccagata tggatttaat ccctgttttg
     1321 cgggtaatat ttttacggtg cgacattacc gtgtggatag aaatattgag agttgaggct
     1381 atttctttgt ttattaagcc ttttacaact gcaataagta cttcttgttc gcgttcggat
     1441 aattcagtgg tatctgtttc ggttatgtca attgtatctt gttcgatgat gtgtctaatt
     1501 tttgcagaga ttgtttgtgc atcatcataa atatcgatga tatcatcgaa ttgttttaga
     1561 aattgactgt cggaatattg actgacaaaa gcaatgatag atgcatgagt acaatcttta
     1621 aaaatagatt tgatagataa gtttttatga aatccaaaca cagctgtatt cacaataata
     1681 atatctgcac gcagttgagc tagtttttga gtaattgtgt ctaattctgt aacgtggtag
     1741 attgactccg ctatgtgcat tcgttctata attgattgca atccagcaaa cacaatagta
     1801 gaaggttcga taatgataat gcgttggttt gtttgtgttg tcataatggc atgtagtaac
     1861 tactcactta gttttgactt ttttctatgc tttcaacata tgggattaac actttatctt
     1921 ctatcaatgt atgtcggttg aggtcttctt cgaataagaa aatatcgtat agtaatttat
     1981 tgcgttcacg ggaagggcaa tcgttgggca catacttaat gataatgttt tttaagtccc
     2041 ctagtttctc ttcaatattg gtgtggtttt tttcaaattg ttggatacca aaacctggtg
     2101 ttttttctcc tgcaatcagt ttatacacat aaggaaaaac gctactatcc tcgtattcga
     2161 ggtgtttgat tacttctttt ttgtatccat caaaaaaagt ttcgataata ctataatgct
     2221 tattggagca ggattggttc attgcatgta tagattgttc tatttctaca atgcgttcag
     2281 ttagatagta ttggtgcgaa ttggatagat acgagagcaa agtgtgtatg tcaattgttt
     2341 gtatttcgct aggtgtaggc atataatcgt cttgcgtata aatagtgcat acgagtagaa
     2401 aaaaatctgt tggaacagca tagtggctac acacttcagc tacggttttt tcgccaaacc
     2461 cgagaggaat accaaatcgg gtaattacgg gcaacagttt gaaattatgc aaaataaggc
     2521 tagctaattt catctgtgga gtaaaatgta tctgtttcat tggcaaagga ataagtatat
     2581 tatgtaggca attaactcat acaaagatag gcattttttt aaggaatcgt ccgcttgcaa
     2641 tcagaaaatt gtaggtaaac gaaccactag tcagtaatgg agatatatcc tacgcggaat
     2701 atgcatacct ttgtattata gtttatctag tttgtttgta gcaaaaaaga ttctctttta
     2761 tcctctaaaa cgtctatcta gttttgtttg taaagtagtg attttagaag tttggtaaga
     2821 tgtatttttc tcgtgattca aatattttct ttacagtatt tatttcgagg aaggtagtgc
     2881 caaaaccgac accaaagctc ccacttaaat aacatacagt atcgttgatg ttaatccatt
     2941 tttcgttaag tagaaactgt aatcctttga tgatgtattg ttgcgtgttg tcgttaccac
     3001 tatcttgaaa aaatggaaaa actccatagg aaagagctaa taatcgggta gatcgttcgt
     3061 gatagcagat agcaaaaatt gggttttgac cacgatacgc cgccaaatag cgagctgtaa
     3121 gtccgctgta ggtgtcagta aaaatggctt ttgaccctaa gttagagcat gctgaaacag
     3181 cagcattgca taggaaagat gtggtgtcgt tggtgcttat ttcaatagga atatcatttt
     3241 ccgccatttt agttttttcg gcttctttgg ctattgttgc catggtgcgt accgcctcta
     3301 ccgggtattt gccataagct gtttcgccac tcagcattaa cgcatccgtg cggtagtaga
     3361 tagcgttggc aatatcagtt acttcggcac gtgttgggcg aggattgtgt atcatggtgt
     3421 gtagcatttg cgtagctaca atcactggtt ttttggcttg tacacatttg cgtattaagc
     3481 ggcgttgaat tccgggtatt ttttcttgag ctacttcaat gcccaaatca ccacgagcaa
     3541 tcattacccc atacgtttgt tccaatattt cgttgatatt atcaaccccc tcttggtttt
     3601 cgatttttga gataattttt attggactgt tgtgttggtc gagtatagct tgaatgtctt
     3661 ttatatcttg tgcattgcgt acaaaagagt gggcaataaa atcgatatca ttttcaattg
     3721 ctattaagat gttttgtttg tcccgttcgg ttaatgaggg taaattgatg cgtacaccgg
     3781 gtacgtttac gctttttcgg ctacctagca taccatcgtt ttgggcttcg catagtaggt
     3841 aatcagttgt tttttcaatt acttttaggt caatttcacc atcgtcgaac aaaatatcgt
     3901 cgtttacatt caagtcacga acaaaaaacg gatagttaac ataaatatgt tcgtgggtag
     3961 atattttatc tggattgcct gaaattttga ttttatcccc tgtttttata tcaatgctat
     4021 tgttttgcac aatggttgtt cgtacttcag gacccttggt atccatcagt atggcaattt
     4081 ggttgctaac ttctctggta ttgtttatga tgcgtaaaaa tccctccaag tcgaggtgtg
     4141 ctgagtttaa ccgcacaaca ttcattcctt cgttgtataa ttggcgtaga aaatctacat
     4201 cgcaacgctt gtcgctgata gtggctatga tttttgtggc tttcgacata aaagcttgtt
     4261 attaaattag ggggaatttt tattcttttt taaaagaaaa acagtgtata ctaaagggta
     4321 attatagagt tattgctgct ttcttttttt tcggatgcaa aaatacaaat aatatcaata
     4381 aaaaactgaa aaacgcgaca aattttctag aaatgatatt tattagagtt aatttgtctt
     4441 aaaacggtgt attttgcttt tatattgaag atgaattgct ttcgattata tgaatagaat
     4501 ctattcccca ttttttatct tgctactata ttggtatata tatcttgcaa taaaaccaat
     4561 aagcaataat ccaaagccag aaacaataac catacatggg agagaaaaag ccattacgat
     4621 acaccctatc agtcctaacc aaggtattat agagccatat atctgttcaa tgcgaggttg
     4681 acgaatagca gctgcattag ctatggtgta atacaataga atggtgaaag tggctgtacg
     4741 tacaacaaat gggaaagaac cgaatagagt gattaacaaa ataattgccg aagtgaataa
     4801 aatggctata tgaggaactg ctgttttttt gtgaatcgat tcaaaaaaat gaggcaaatc
     4861 attgcggcga cccattgcca aaagcatgcg gctaatacct actatctggc ttagtagtac
     4921 gcccaacata gctgttgagg ctcctaaggt gataacaaca gacatacctg gagcttgtat
     4981 cctgtctgct gctagttgta gtggagaact cgtacttgcc atagatgggg ctcctaatac
     5041 tcccgtagcc actatagata cgccaacata tagtagtatt gccacggcta tagttataat
     5101 caccgctttg gggattgttt ttcgtgggtt gcgaacttct tcggctaatg tggctatacg
     5161 tgcatatcct gtgaaagcaa aaaataagat agctgcagat tcggctattc ctttccatcc
     5221 aaatggagca aaaggaatga aatttgttgt atcaacactt ggaataccac caactacaaa
     5281 atatagtagt gataataagg tgatgagtac aatagcaata tttatcgttc ctactttttg
     5341 tatgccccac aaattggcaa acgtaagcaa tacaatggct gcaatagaat attcgatggg
     5401 agagaaatag ggtataagtt gatggaaata gcttccaaaa cctatagcca caatgcctcc
     5461 tgctgatagt ttgcttaata caaacatcca tcctgcagaa aagcctaacg aaggggttaa
     5521 caagcgatac ccatattcgt acgtacctcc agattgagga taaacggcag ccaattgtga
     5581 tgaactgagc ccattaaaag tggctattgt acctgctatc aataagccaa tgataaaaga
     5641 aggtccagat acaccagcag caactcctgt aacaacaaat atacctgctc caataatcgc
     5701 tcctaagcca acagctacgg catcttttaa gtctaaactc ctttttagtt tatttagttt
     5761 catagtgtgt atgtttgttt aaccctatcc ccacaatgtc tgtaagtata aaatcgttgt
     5821 tatctatgta caaaaatatt gaaaaagaga ataactatat ggtataattg tactaaaaaa
     5881 tggtattttt gaagaatata cagtatggtt ggttgtgtgt acgatttttt tcgttgacta
     5941 gtatgctatg tttgcccatt cattttgaaa atagaaaagg agatatcgtt acgttgaata
     6001 ttctccgaaa aaagtataaa aaatcagtag tatttgtaaa cttttcacga actttgtagt
     6061 tcttccaaaa aaaacaacaa tgattcagaa acattctcca ctaagtcttg gtacagaaag
     6121 tattggtaag ctgttggcac aatatgctat tcctgctatt gtagcgatga cagcttcttc
     6181 tctatacaat atcacggata gtgtttttat tgggcatgga gttggagctc ttgctatatc
     6241 aggtttagct cttacttttc cggttatgaa cttagctgcg gcatttggtt cgctcgttgg
     6301 agcgggagct tctgctctta tgtcgatgcg attaggtcaa aaagactatg acactgcgaa
     6361 taaagtacta gggaacgtat ttgtgctgaa tattattatg gggttggcat ttacaggcat
     6421 catgcttgtt tttctgaaac ctattttgta tttttttggt gctagcgaag caacactgcc
     6481 ttatgcgtat gattatcttc aggtaattct ttacggaaat gtagtaacac atatgtatat
     6541 gggtcttaat gccttgttgc gttcatcggg tcgtcctcag aaggcaatgt atgcaacgat
     6601 atgttcggtt attcttaatt taggacttac cccattattt atttatgttt ttggatgggg
     6661 tattaaaggg agtgcttggg ctactgtact gtcacaactc ttgatgttga tatggcagat
     6721 aacaattttt agtaataaaa agaattttat tcatttgaag aaaggagttt ttcgattaga
     6781 aaaaaagata gttctcgatt cgtttgccat tggattatct ccttttttta tgaatgcagc
     6841 gtcatctgtt attattatcg ttatcaatca ggcattaagc cgtcatggtg gggatttggc
     6901 agtaggtgct tatggaattg ttaaccgtgt tgttttcctt tttgccatgg tagtattggg
     6961 acttaatcag ggaatgcaac ccattgcagg gtataatttt ggtgcccagc attatgatcg
     7021 tgtagataaa gtgctgcgta caactatttt gtatgccaca ggcattatga tttttggatt
     7081 tattataggg caatttttcc cattttctgt tgcatctgta tttaccaccg atgtgcaact
     7141 taccgaacat gcagtaaaag gcttgcgtat tgtgatgatt ttttttccaa ttattggatt
     7201 tcagatggta acgggcaatt tttttcaaag tattggtcag gcaaaaaaag ccatttttct
     7261 ttcacttact aggcagatga tatttttgtt gccttgtttg cttattttcc cttccatatg
     7321 gggcgtgaaa ggtgtttggt ttagtttgcc tacagctgat ctagcctcaa gtttggttgc
     7381 aggatggttg cttagtaaac aacttaaaca atttaaaagg agtaaacaga ctaagtcgtg
     7441 tgatgaagta gggatttaat tcaacgttat taggaggatt tttctatgaa caacaatttt
     7501 attattacca ttggtagaga attaggaagt ggaggtcgca ttataggagc tcaattggcg
     7561 aaaaaactga atagtgcgtt ttacgacaaa gaattaattc taagagcctc caaagaaagc
     7621 ggtctgtcta gctcttttat ggagcaagtt gatgagcatt ttagtcctat tcagacagga
     7681 gggcttttgg gcgatttttt tcaacaattt tattttagta gtgaatcttg ttttcaaatc
     7741 caaagtgatg ttattcgtga gattgctgaa catgaatctg ccgtaatagt aggacgtgga
     7801 gctgattatg tgttgagaga acatccgcgt tgtttgaata tttttgttac agctaacatg
     7861 agtgatcgta tacaacgtgt tgccgaatcg catacgataa gtgaagatga agcacgtatc
     7921 cttattgaaa aagccgataa aaagcgtgct tctttttaca attattttac gagcaaagaa
     7981 tggggaagag caaaatcata tgatttgtgt gtaaattctt cggtatttgg attggaaaaa
     8041 acaaccgact acatttttca gcttgtgaaa gatagatttt ctccaatacc ctaatcatta
     8101 agatgcatac ttgttagtga attgtagcta cacactatca aatacataat aaaagaaaac
     8161 aagatttttt tcgatatcgt gtgagaggct ctccccatca gtctttactg gtggggcagt
     8221 ctactcgatt atgggcagac gtttagtata aattttctca gtcattttct tcgtaataat
     8281 acgagtaatc tattcctttc ataaacattt cgcggtcatt aaccttgtcg gtcaaactgc
     8341 tttctagtat tttctttaaa gcactactgt ttagtggact ttgtatcatt gcattcatat
     8401 agactttttt tcctatttta ctccaatcaa cgcatttgtt cagacgtttt ttcagaatta
     8461 aatccaacca gattcgggta cttctgccat ttccttccat aaatgggtgc gccatgttca
     8521 tttccacata cttatctact atctcttcaa atgtagtttc tggcatttct tctattagct
     8581 tcaatgtttc gcctaaaaat tgtgatacag caaattggaa accgcctttt gaaatgtttt
     8641 tctgccttat ttgtcctgca aaatcataca gtccgccaaa caaataagcg tgaatttgtt
     8701 gtaaaccttt agcggttcca acttcaatgc tgtttataaa ggtactttcg aataaagcat
     8761 acgctttggt tttacttttt ccatcgatac tttcatcact gtatgtgaac cattcgataa
     8821 atcggttagc tttttttccg gggaattctt tacccaatgc aataataccg tcataatcta
     8881 acatgtcggt taaatacctc ttaccgtcat ttgctaaaag tttcagttgg gtagtggcac
     8941 taaccaactc actattttct ttctttagct tggctttgag atatttccag taattacggg
     9001 ttttagtata atcattttgg tcagtaagca cagcaacaat atctaaaaca gagaacctcc
     9061 atttagcatt ttcttcgtcc caaagagcac gtacttcacg gtcatcaaaa aaacgtattg
     9121 atattttggc tttactcatt ttttcaaatt tgtcttgatc tgttgaaaat atttgtcgtt
     9181 gaacagcaaa attacaaatt taaatcaata gacagttttg ttttactttt tgttgttatt
     9241 ttcttattac ttaccacttt tcaaacgtat aatttctaaa taattctctg atttttagaa
     9301 ttttctagta aaggtcaatt tgtgcttaaa atcaatgttt ttgttttttt ttacgattgc
     9361 ccataacgac agtctgtcta aaaacctccg tttagtttaa tttttgctca aatgtaatgg
     9421 gttt
//
LOCUS       DAOSRP010000076         9256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7153_length_9256_cov_5.056298, whole genome shotgun sequence.
ACCESSION   DAOSRP010000076 DAOSRP010000000
VERSION     DAOSRP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7153_length_9256_cov_5.056298"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..575
                     /locus_tag="PLJ82_05855"
     CDS             117..575
                     /locus_tag="PLJ82_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41066.1"
                     /translation="MKKILILLLLLYFSCFYTFSATFDENRIATGKNKFGFAVGYGNQ
                     LFWDVTYNYQLLLYSLQYQYTFYQKNSFSSELVIQPQYNAASFFEDTQKYSFEYGVNI
                     GALLRKELSNFSVFAMLSAGPHYVSDVPSRQANGFIFSDNLMVGVSYITY"
     gene            709..1833
                     /locus_tag="PLJ82_05860"
     CDS             709..1833
                     /locus_tag="PLJ82_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41067.1"
                     /translation="MKHLEIRTIEQVKSLFHTTDNIQYVAFQHIDFNDVSTSKFDKSY
                     SNCLFLGCILSQEFICKKLRDCTIFPSIDVPYTIFIPHLYTPHDLYAGYKIGSPDSFS
                     HTFDQQVYSHYIAKGKETSDIKETLARRIHDHSISDALHDFLVSYEPTKLVAIMGGHN
                     IARTDIGFLEIAKMSKTLTEQSYLMLSGGGPGAMEATHFGAWMAGRTESELLHAYEML
                     SEAPHYDHLLWLEKAFMVMERYPQHTYESIGIPTWFYGHEPPTPFATRIAKYFENSIR
                     EDGLLAIAKGGIIFTPGNAGTIQEIFQDANQNHYLTFGLASPMIFYNADYWTHSRPIY
                     PLLTQLFEEGKYKNLILSVCNNQQEVLEQLAAFALSQQAE"
     gene            2125..2814
                     /locus_tag="PLJ82_05865"
     CDS             2125..2814
                     /locus_tag="PLJ82_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HOR41068.1"
                     /translation="MWLINSSIGRKLIMSISGIMLVLFLLFHGLMNMVVIFSADAYNA
                     ICAFLGANWYALIATKGLALGFLVHIAYALFLSYQNMKARGKERYAVSDKQDNVSWAS
                     KNMLVLGLIVFGFLALHLYHFWSKMQLVEIGHMLGKHVGDIDKAVDGAYWITYWFSQP
                     VYAIIYLVWLSALWLHLTHGVWSSLHSLGLNNQTWLPRVKCISNIFATLVVLLFVSVV
                     VYTYVYQNFVL"
     gene            2905..4842
                     /locus_tag="PLJ82_05870"
     CDS             2905..4842
                     /locus_tag="PLJ82_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOR41069.1"
                     /translation="MIDAKVPSGPLAEKWKNYKAHQKLVNPSNKRRLDIIVVGTGLAG
                     ASAAASLAELGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRARESNVYRLAEVSGSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYAKGQ
                     TGQQLLLGAYSALSRQVGKGAVKLYSRYEMLDVVIIDGRARGIIARNLVSGKIERFFA
                     HAVVVGTGGYGNAFFLSTNAMGSNGSAAIQMYKKGAYFANPCYVQIHPTCIPVHGEFQ
                     SKLTLMSESLRNDGRIWVPKKKEDAEAIRKGTLKPTDLKEEDRDYYLERRYPAFGNLV
                     PRDVASRAAKERCDAGYGVGATGLAVYLDFADSIKRLGLDVVKARYGNLFQMYEKIVD
                     DNPYETPMMIYPAIHYTMGGIWVDYELQTSVKGLFCIGEANFSDHGANRLGASALMQG
                     LADGYFVLPYTIQNYLADQIQVPRMSTDLPEFIEAEKEVNDKIQKLLSIQGQRSVDSI
                     HRELGLVMWDFVGMGRNKEGLQQAIDKIKAIKKEFWTNVYIPGKGDDLNVELEKALRV
                     ADFIEIGNLMARDALQREESCGGHFREEYQTPEGEAMRNDDDYAFAACWKYMGENADP
                     VLLKERLDYEFIQRQQRNYKA"
     gene            4879..5634
                     /locus_tag="PLJ82_05875"
     CDS             4879..5634
                     /locus_tag="PLJ82_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOR41070.1"
                     /translation="MEKTINVTLKVWRQAGPKAQGKFETYKLNNVSTDSSFLEMMDIL
                     NEQLISERKEPVAFDHDCREGICGMCSLYINGHPHGPDGEITTCQLHMRKFKDGDTIT
                     IEPWRSAAFPVIKDLMVNRGAYDKIIQAGGFVSVNTGGVPDGNAIPISKIYADEAMDA
                     ASCIGCGACAAACKNGSAMLFVAAKVSQLALLPQGKVERATRAKAMVAKMDELGFGNC
                     TNTGACEAECPKNISISHIARLNREFLSAKIKD"
     gene            complement(5705..6475)
                     /locus_tag="PLJ82_05880"
     CDS             complement(5705..6475)
                     /locus_tag="PLJ82_05880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOR41071.1"
                     /translation="MKTVLITGASEGIGYELSQLFANAHYQLILVARNEQKLNAMCET
                     YTSNGIKCTYFVADLSIMSHVERLADKIDQLPFKIDYLINNAGFGIYGNSTELDWEKE
                     SAMYQLNMISLAYLTKHFSIQMTKNGFGRILNVASIATFQPLPFMAGYAASKAFVLSL
                     SQALNEELKKQNVYVSTLCPGVTESKFHNTANTEKKLLGASFFPVATAQEVAQYGYKL
                     LMSGKNIGIYGLLNRIQIGLLRFIPRAFIVKIVGIMMK"
     gene            6633..8600
                     /gene="dnaG"
                     /locus_tag="PLJ82_05885"
     CDS             6633..8600
                     /gene="dnaG"
                     /locus_tag="PLJ82_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR41072.1"
                     /translation="MIDQVTVDRILQTAQIEDVVGEFVSLRKRGVNMIGLCPFHDEKT
                     PSFTVSPAKNICKCFGCGKGGTPVNFIMEHEHLSYYDALRYLAKKYNIEIVEKEISAE
                     ELNKRNERESLFVINTFAQTYFEDVLWKQDEGRSVGLSYFKERGFRDDVIKKFGLGYS
                     LESYDSFGRVALEKGYKRDYLLKTGLCTERQDASLVDRFRGRVIFPVHTLSGKIVAFG
                     GRILKTDEKTAKYLNSPESEIYHKSNELYGLFFAKQSIVKYDNCFLVEGYTDVLSMYQ
                     SGIENVVSSSGTALTPGQIRLIRRFTSNITVLYDGDAAGIKASIRGIDLLLEEGMNVK
                     VVLLPQGEDPDSFAKKQNASDFIQFIEKNQTDFIHFKSHLLLGEAGKDVSKKAKLIAD
                     IVQSIAIIPDAIMRSVYVKECAKQFEMNENVLFTEVNKRRILLLESQSKQTNFVVESE
                     DADNAISELPSEHSTVNAFLHYTFLQEQNLIACILRNGASKIVGQDENKAEYIISVFD
                     FILNDLAVDDLDFSYSLHQQICNELIRLRASNQEQIAFYFLNHPNKEIRDYVFELITD
                     KHTLSKVHQKYKAIESEQDRLNYQVTKYLLDFKEKIVDYYCKELRDKIKQSEGKVSDE
                     EMFSLLQELNRYNAIRNIIMKQSGEKTVIKI"
     gene            8619..>9256
                     /locus_tag="PLJ82_05890"
     CDS             8619..>9256
                     /locus_tag="PLJ82_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR41073.1"
                     /translation="MHYTIEDLIKLPIWQHFYAISQIPRPSGRMDAITDYIEGFGKSL
                     RLETLRDKANNIVIRKPASAGYEGCKKVILQSHLDMVPQKNEGVLHDFCTDAIKWKIT
                     DNWLMATDTTLGADNGIGVAAMMAILENKELVHPPLEALFTVDEENGMNGAFGVAPDW
                     LQGDILINLDSEDEQEIIVGCAGAVRVQCNFNFEKTPVPEGDKAYKIGIKGLL"
BASE COUNT         2998 a         1320 c         1916 g         3022 t
ORIGIN      
        1 ccaatagcgt cgatggtact gcacttgtgt gggagagtag atagccgccg tttttcaaaa
       61 ggaggtgatg tgtcatacat cgcctccttt ttttatttag gggtaacaat agtgaaatga
      121 agaagatttt aatacttttg cttttacttt atttttcatg tttctataca ttctctgcta
      181 cttttgatga gaatcgtatt gcaactggta aaaataaatt tggatttgca gttggttatg
      241 gtaaccaatt gttttgggat gtaacctata attatcaatt actattgtat tctcttcagt
      301 atcagtatac attttatcaa aagaattcat tttcaagtga acttgtaata cagccacaat
      361 ataatgctgc gagttttttt gaggatacac aaaaatattc atttgagtat ggtgtgaata
      421 taggagcatt actaagaaaa gaattgtcaa atttttctgt ttttgcaatg cttagtgctg
      481 gtcctcatta tgtttctgat gttccatcaa gacaagccaa tgggtttatt ttttctgaca
      541 atttaatggt tggagtttct tatataacat attagtaatg ccggtttcat cgcccctaat
      601 ggaggtgtta ataatactat ttttcaaata ggattctttg ttttaccata atttgtacga
      661 ttaagtccaa tatttttgtt atttttacat gaaataaaag aaatgggcat gaagcatttg
      721 gagattcgta caatcgaaca agtaaaatct ctctttcata ctactgataa tattcaatat
      781 gtggcttttc aacacattga ttttaatgat gtaagtacta gtaaatttga taagtcgtat
      841 tcaaattgtt tgtttctagg ttgtattctt tctcaagaat ttatctgtaa gaaattacga
      901 gattgcacta ttttcccttc aatagatgtc ccttatacta tttttattcc acacttatat
      961 actccacatg atctttatgc aggatataaa attggaagtc ctgattcatt ttcgcatacg
     1021 tttgatcaac aagtatattc ccattacata gccaaaggga aggagacttc agatattaag
     1081 gaaacattag cacgtaggat acacgaccat tcaatttctg acgcattaca tgacttttta
     1141 gtttcttatg aacctaccaa attagttgca ataatgggtg gtcataatat agcacgaaca
     1201 gatattggat ttttagaaat agcaaaaatg agtaagactt tgacagagca atcttacctt
     1261 atgttatctg gaggaggtcc aggggcaatg gaagcaactc attttggtgc ttggatggct
     1321 ggtagaacag aaagcgaatt actacatgct tatgaaatgc tttcagaggc tccccattac
     1381 gatcatcttt tatggttaga gaaggcgttt atggtaatgg agagataccc acaacacaca
     1441 tacgaaagca ttggtatccc aacatggttt tatggacatg aacctccaac tccttttgca
     1501 acacgtattg caaagtattt tgaaaatagt atacgagaag atggtttgtt ggcaatagca
     1561 aaaggaggta taatttttac cccaggaaat gcaggaacta ttcaagagat ttttcaagat
     1621 gccaaccaaa accattatct tacctttgga ttagcaagtc caatgatatt ttataatgct
     1681 gattattgga ctcatagccg accaatatac ccgctcttaa cacaattgtt tgaagaaggt
     1741 aagtataaaa atcttattct ttctgtatgt aataatcaac aagaggtgtt ggagcagtta
     1801 gccgcttttg ctctgagcca acaagctgag tagcataata tcctgcttgt tgtgttattt
     1861 tgagcaaatt ttacatatat ttacctattt ttgtatgctt aatgaaagca atacagtttt
     1921 ttgggctaca aattgatttt atattagctt aaataacaat aacttaaact atttttggga
     1981 gaattactat aagaatgaaa aatcgtttgc attacgttaa aaaatgtgat agtatatacc
     2041 ctttagcatt gaacttttta ttacttttgt acactgaaaa ttaaattttt gtattaaatc
     2101 aataataatt aaaaaactgg aagtatgtgg ttaattaact cttcgatagg aagaaagctg
     2161 attatgagta tctctggcat catgctggta ctttttttgt tgtttcatgg tctaatgaac
     2221 atggtagtga ttttttctgc tgatgcatat aatgcaattt gtgcattttt gggtgcgaat
     2281 tggtatgcct taatcgcaac taaaggttta gctttagggt ttttagtgca tattgcttat
     2341 gctttatttt tgtcttatca aaacatgaaa gctcgtggta aagagcgtta tgctgtttca
     2401 gataaacaag ataatgtaag ttgggcttct aaaaatatgt tagtgttagg attgattgta
     2461 tttggatttt tagcattgca tttgtatcat ttttggagta aaatgcaatt agttgaaatt
     2521 gggcacatgt taggaaaaca cgtaggagat attgataaag cggttgatgg agcatattgg
     2581 attacttatt ggttttctca accagtgtat gctattatct atttagtgtg gctttcagct
     2641 ttatggcttc atcttaccca tggagtatgg agttcattac attcattagg attaaacaat
     2701 caaacatggc ttcctcgtgt gaagtgtatt tcaaacattt ttgcgacatt agttgtatta
     2761 ctgtttgttt ctgttgttgt ttatacttac gtatatcaaa attttgtttt ataatttgta
     2821 actagaaata tatcacacaa tatggctaag aaagaaactg ttgtagaaac aactcaggct
     2881 gtgacgccta cagaaacacc aattatgatt gatgccaaag tgccttctgg tcctttagct
     2941 gagaaatgga agaattacaa ggcacatcaa aaattggtaa atccatcgaa taaaaggcga
     3001 ttagatatca ttgttgtagg tactgggtta gcaggagcct cagctgctgc atcgttggct
     3061 gaattaggtt ttaacgtact aaacttttgt attcaagatt caccaaggag agcccattct
     3121 attgctgcac aaggaggtat aaatgcggct aaaaattatc aaaatgatgg agattcagtg
     3181 tatcgtttgt tttatgacac aataaagggt ggtgattata gagcaagaga gtccaatgtt
     3241 tatcgtttgg cagaagtatc aggaagtatt attgaccaat gtgttgcaca aggagttcct
     3301 tttgcacgcg aatatggagg tttgttagat aatcgttcgt ttggaggagc acaggtatca
     3361 cgtacttttt atgctaaagg tcaaactggt caacaattat tattaggggc atattcagca
     3421 ctaagccgtc aagttggcaa aggagccgtg aagctatatt cgcgttacga aatgttagat
     3481 gtagtaatta ttgatggaag agctcgagga atcattgcac gtaatttagt ttctggaaaa
     3541 attgaacgct tttttgccca tgcggtagta gtagggacag gtggttatgg taatgctttc
     3601 tttttgtcga ccaatgcgat gggatctaat gggtctgctg ctattcaaat gtataaaaaa
     3661 ggagcgtatt ttgctaatcc atgttatgtc caaattcacc caacatgtat tcctgtgcac
     3721 ggtgagtttc aatcgaaatt aacattgatg tcggaatcgt tgcgtaatga tggtcgtata
     3781 tgggtgccaa aaaagaaaga agatgcagaa gctattcgca aagggacttt aaaaccaact
     3841 gatttaaaag aagaagatag agattattat ttagagcgtc gttatcctgc ttttggtaat
     3901 ttggtgcctc gtgatgtggc ttctcgtgct gccaaagaaa gatgtgatgc tggatatggt
     3961 gttggtgcta caggtttagc tgtatattta gattttgctg attcaattaa acgtttaggt
     4021 cttgatgttg ttaaggctag atatggtaac ttatttcaaa tgtacgaaaa gattgtagat
     4081 gacaatccat atgaaactcc aatgatgatt tatcctgcca tacactatac aatgggaggg
     4141 atttgggtag attatgaact acaaacatcg gttaaaggat tgttctgtat tggcgaagca
     4201 aatttctcag accatggagc aaaccgttta ggtgcatctg ctttaatgca aggtttagca
     4261 gatgggtatt ttgtattacc ttatacaatt caaaactatt tggcagatca aattcaagta
     4321 ccacgaatga gtactgattt gcctgaattt attgaagcag aaaaagaggt gaatgacaag
     4381 attcaaaaat tattaagcat acaaggacaa cgttctgtag atagtataca tagagagcta
     4441 ggtcttgtta tgtgggactt tgtgggtatg ggcagaaata aagaaggatt gcaacaggct
     4501 attgataaaa ttaaagctat taaaaaggaa ttttggacta acgtatatat tcctggtaaa
     4561 ggagacgatt tgaacgttga attagaaaaa gctttgcgag tagcagattt tattgaaata
     4621 ggaaatttga tggctcgcga tgcattgcaa agagaggaat catgtggagg tcacttccgt
     4681 gaagaatatc aaactccaga aggagaagca atgcgtaatg atgatgatta tgcatttgcg
     4741 gcgtgttgga agtatatggg agaaaatgca gatcccgtgt tgttaaaaga aagacttgat
     4801 tatgagttta ttcaacgtca acaacgcaat tacaaagcat aattaacaag gtatccaaac
     4861 aaaaagaaat tctgtaagat ggaaaaaaca ataaatgtaa cgcttaaagt ttggcgtcag
     4921 gcaggtccaa aagcacaagg taaatttgaa acctataaat tgaataatgt ttcaacagat
     4981 agttcattct tagaaatgat ggatattttg aatgagcagt taataagcga acgcaaagaa
     5041 ccagtagctt ttgatcatga ttgtagagaa ggtatttgtg gtatgtgttc gttgtatatt
     5101 aacggacatc cacacggacc agatggtgaa atcactacat gtcaattgca catgcgtaaa
     5161 tttaaagatg gagatacgat tactattgag ccatggcgtt cggcagcatt tcctgttatt
     5221 aaggatttga tggttaatag aggggcttat gataagatta ttcaggctgg tggttttgtg
     5281 tctgtaaaca caggtggagt gccagacggc aatgcaattc ctatttcaaa aatttatgca
     5341 gacgaagcaa tggacgctgc ctcttgcatc ggttgtggtg cttgtgcagc ggcttgtaaa
     5401 aatggatcag caatgctatt tgttgctgca aaggtaagtc aacttgcatt attgcctcaa
     5461 ggaaaagtag aaagagcaac acgagccaaa gcgatggttg ctaaaatgga tgaattaggg
     5521 tttggtaatt gtactaatac cggagcttgt gaagcagagt gtcctaaaaa tatttccata
     5581 agccatattg ctcgtttaaa cagagagttt ttgtcggcta agataaaaga ttaatcgcta
     5641 ggtttgtcaa taaaaaagct gctttgtaat tcacaaagca gcttttttat tgaacgaagt
     5701 caatttattt catcattatc ccaacaattt tcacaataaa tgcacgaggg ataaatcgta
     5761 acaagccaat ttgaatgcga tttagcaagc catatatgcc gatgtttttg cctgacataa
     5821 gtagtttgta cccatactga gcaacttctt gtgctgtggc aacagggaaa aaggaagctc
     5881 ccaagagttt tttttctgta ttggctgtgt tatgaaattt actttctgta acacctggac
     5941 atagggtaga cacatatacg ttttgctttt taagttcttc gttcaatgct tgagataagc
     6001 taagcacaaa agctttggag gctgcatacc ctgccataaa tggcaaaggt tgaaaggtgg
     6061 caatagaggc aacatttaaa atccttccaa aaccattttt tgtcatttga attgaaaagt
     6121 gttttgttaa ataggctaaa ctaatcatat ttagttgata catagcagat tctttttccc
     6181 aatccagttc agtagagttt ccatatatcc caaacccagc gttgtttatt agatagtcta
     6241 ttttgaatgg cagttggtcg attttatctg caagcctttc cacgtgagac ataatggata
     6301 aatcagccac aaaatacgtg cattttatac cattagatgt atatgtttca cacatggcgt
     6361 ttaatttttg ttcatttctt gccaccaaaa taagctgata atgtgcgttg gcaaataatt
     6421 gagacaattc gtatcctata ccttctgatg cccctgttat aagtacggtt ttcataagtt
     6481 ttgaagttta tttattactc gatattcttg ttgcgataaa tttttgttga gatgccctac
     6541 aaatatacga gattttttgt agaaaaaagc tcctacatca agatagataa atagtaaaat
     6601 tctcgtatat ttgtgttcct aacaaaatag agatgattga tcaagtaaca gtggatagga
     6661 ttctacaaac agcacagatt gaagatgttg ttggcgagtt tgtatcactt cgtaaaagag
     6721 gggtgaatat gattgggctt tgtccatttc atgacgaaaa aacgccatcg tttactgttt
     6781 ctccagccaa aaatatttgt aaatgttttg gttgtggaaa aggaggaacg cctgtaaatt
     6841 ttatcatgga gcatgagcat ttatcatatt atgatgcgtt gcgttactta gctaaaaaat
     6901 ataatattga aatagtagaa aaggaaatat cggcagagga gttaaataaa cgcaacgaaa
     6961 gagaaagttt gtttgtaatt aacacttttg ctcaaactta ttttgaggat gttctttgga
     7021 aacaagacga gggaagatcc gtgggtttat cttattttaa agaaagaggt tttcgtgatg
     7081 atgttataaa gaaatttggc ttaggttata gtttggaatc gtatgattct tttgggcgtg
     7141 tagctttaga aaaaggctat aagcgggact atcttttaaa aacaggattg tgtactgaac
     7201 gtcaagatgc gagtttggtt gatagatttc gaggaagggt catttttcct gtccacacat
     7261 tgtcgggaaa gattgtggct tttggaggta gaattttgaa gactgatgaa aaaacagcaa
     7321 aatacctcaa ttcaccagaa tctgaaattt atcataaaag taatgagttg tatggattgt
     7381 tttttgctaa gcaaagtatt gtaaagtatg ataattgttt tttggtagaa ggttatacag
     7441 atgtattatc tatgtatcaa agtgggattg agaatgttgt atcatcttca ggaaccgctc
     7501 ttactcctgg tcagattcgt ttgatacgta ggtttacctc caatattacg gtgttatacg
     7561 atggagacgc tgctggaata aaagcgtcta ttaggggtat tgatttattg ctagaagaag
     7621 gcatgaatgt aaaagtggtg ttattgccac agggagaaga cccagactct tttgctaaaa
     7681 aacaaaatgc cagtgatttt atacagttta tagagaaaaa tcaaacagac tttattcatt
     7741 ttaaatcaca cttgttgctt ggagaagccg gcaaagatgt gagtaagaaa gccaaactaa
     7801 ttgcagatat tgtgcaaagc attgcgatta taccagatgc tattatgcgt tctgtatatg
     7861 taaaagagtg tgctaaacaa tttgaaatga atgagaatgt tttgtttaca gaagtaaata
     7921 aaaggcggat tcttttgctt gaatctcaat cgaaacagac aaattttgtg gtggaatctg
     7981 aggatgctga taatgctatt agcgaacttc cttcagagca ttcaacagtg aatgcatttt
     8041 tgcattacac ttttttgcaa gaacaaaatc taatagcctg cattttacga aatggagcat
     8101 ctaagattgt aggtcaggat gaaaacaagg cagagtatat tatttcagtt tttgatttta
     8161 ttttaaatga tttagcggtt gatgatttag atttttccta ttctttgcat cagcagatat
     8221 gtaacgaatt aattagattg agagcaagca atcaagaaca aatagcattt tattttttaa
     8281 atcatccaaa taaagaaatt cgggattatg tctttgaact aattacggat aaacatactt
     8341 tgagcaaagt tcatcaaaaa tacaaagcta ttgaatcgga gcaagataga ttaaattatc
     8401 aggtaaccaa gtatttactt gattttaaag agaaaattgt agattattat tgcaaagagt
     8461 tgagagataa gataaaacaa tccgaaggga aagtgtcgga tgaagagatg ttttctttat
     8521 tgcaagaatt gaatcgatac aatgcaatac gaaatatcat aatgaaacaa tctggcgaaa
     8581 aaacggtcat aaagatttaa ctataacttt tttaattcat gcattataca atagaagatt
     8641 tgataaaatt gccaatatgg caacactttt atgcaattag ccaaattcca cgtccatctg
     8701 gtaggatgga tgccatcact gattatattg aagggtttgg aaaatcattg cgtttagaaa
     8761 cgctacgaga caaagcaaat aatattgtaa tacgaaaacc agcaagtgca ggatatgagg
     8821 ggtgcaaaaa ggtcattttg caaagccatt tggatatggt gccacaaaaa aatgaagggg
     8881 tattgcatga tttttgtact gatgctatta agtggaagat tactgataat tggttgatgg
     8941 caacagatac aacccttggg gcagataacg ggataggagt tgcagcaatg atggctattt
     9001 tagagaataa agaacttgtg catcctccac tagaggcatt gtttacagta gatgaagaaa
     9061 atgggatgaa tggagctttt ggagtagccc ctgattggtt gcaaggagat atacttatta
     9121 atttagattc ggaagacgaa caagaaataa tagttggatg tgcaggagca gtgcgagtac
     9181 aatgtaattt taattttgaa aaaactcctg ttcccgaagg agataaagca tataaaattg
     9241 gaataaaagg gcttct
//
LOCUS       DAOSRP010000077         9173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7223_length_9173_cov_4.764861, whole genome shotgun sequence.
ACCESSION   DAOSRP010000077 DAOSRP010000000
VERSION     DAOSRP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7223_length_9173_cov_4.764861"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLJ82_05895"
     CDS             complement(<1..488)
                     /locus_tag="PLJ82_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOR41074.1"
                     /translation="MKTLDELVKNIAVIRRLHYTSDRQINGLCFDSRQVQQGYLFFAT
                     KGSAVDGHQYIADAIELGATAIVCEQLPTSIHSDIAYLQVADSAVAMGNMAANWYDNP
                     SQKLTLVGVTGTNGKTTIATLLYNLFEQLGYVSGLLSTVCNYVHKEQIPSTHTTPDAL
                     SI"
     gene            complement(499..>1680)
                     /locus_tag="PLJ82_05900"
     CDS             complement(499..>1680)
                     /locus_tag="PLJ82_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOR41075.1"
                     /translation="ALEDGVVDTTVTVNTGNGLKQFGKRTMRDWNANKGGFGVMSMPK
                     ILYQSSNVGTSSIIYDHYAHNPQRFIDLLKRTKIHQAGDLGIPGHGHVKIKDPSMKSW
                     SYTTLPWMSIGYEIQIPPIYTLMYYNAFANNGKMMKPYFTKEIRQHGITVEKYEPIII
                     NPKICSDATLGKIQSMLEGVVTKGTGKAIKSKLFPIAGKTGTAQIAIGGQYKQGGQKA
                     KLQISFAGYFPADKPMYSMIVYIKDPQGPHISAGGMSGAVFKNVAEKVYTMKLGDIPS
                     WAHSEVTDSLAIIKKGYVPHINEVLKTMAKPIKMADKTMQWGEYIVNDSTQIASFNPF
                     VMREHTMPNTIGMSAKDAVYIVEKMGLKVTIIGFGKVVEQTIPENEHIVQGMPVTLTL
                     N"
     assembly_gap    1681..1780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1781..2598)
                     /locus_tag="PLJ82_05905"
     CDS             complement(<1781..2598)
                     /locus_tag="PLJ82_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41076.1"
                     /translation="MKAVLFILLAIAVVAIISLLVLLFIRKNTVKKEIMLRFAIIYIF
                     ILIAFGGVIFRIVHLQYVEGEKLRALTASQQSALRPIKPNRGNILASDGRLLASSIPT
                     YYLYMDLMADALVQKSTKNEAEFKQDVRRLSVALAKKFKDKTAWEYEKQFLNTFYDKT
                     NKRKREYLLYPKPISYIDLAEVKTFPILQKGRYGGGFYTKKRVTRIKPFGELASRTIG
                     DIYGEEEKGGSSGLEKQYNKLLQGEEGFYYRRKMAGKFVDIVVKDAVNGYDVVST"
     gene            complement(2603..2977)
                     /locus_tag="PLJ82_05910"
     CDS             complement(2603..2977)
                     /locus_tag="PLJ82_05910"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOR41077.1"
                     /translation="MAKIKTLKKKIKESEEFNDLTKTSVKDIFDGNVFSKSYFLKQIK
                     LIVLVSFFIFFYMDNRMECEKQIATINDLNQQLTDVKYTSMVVEADLLSISRRTQIEA
                     LLHQHAIELKELSIPPYKLTTK"
     gene            complement(2982..3884)
                     /gene="rsmH"
                     /locus_tag="PLJ82_05915"
     CDS             complement(2982..3884)
                     /gene="rsmH"
                     /locus_tag="PLJ82_05915"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR41078.1"
                     /translation="MTTYHTPVLLKESIEGLLTNKSGVYVDLTFGGGGHSRFLLSQLH
                     SDAKVFGFDQDKDAEMNAVNDSRFTFVQSNFCYLENFMQYYGINEVDGILADLGVSSH
                     HFDDAERGFSFRFDGILDMRMNQAAKLTAKEILNTYTEQQLADIFFYYGELPLARKIA
                     RKIVEQRKNEQFETIFGFVGIIQPLIGKEKEKKQLAQVFQALRIAVNNEMDALKKMLL
                     SSAKVLKKEGRFVCITYHSLEDRLVKNYLKTGNMEGIINKDFFGNIQTPFSLINNKVI
                     IPTTEEIASNSRARSAKLRIASKK"
     gene            complement(3977..4435)
                     /locus_tag="PLJ82_05920"
     CDS             complement(3977..4435)
                     /locus_tag="PLJ82_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOR41079.1"
                     /translation="MTSLIGNIEAKVDAKGRAFVPAQFRRQLQDETKFILRKGLFHDC
                     LVLYTEQAWNDIITVLRNNLSRWNRREEQIFRQFVAEADRIELEENGRMLIPKRYIDA
                     LQIDKDIRFVGADTVIEIWPINKVENTFIDADNFALEIENLMNIGKPTKD"
     gene            4629..5444
                     /locus_tag="PLJ82_05925"
     CDS             4629..5444
                     /locus_tag="PLJ82_05925"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR41080.1"
                     /translation="MSQPVQIDIASILKGKTGGKKPSSMLVYLLSKLLHVKEINHFLT
                     LAKDKKNLDFFQALIEYLNVRVLVSGVDNLPLSGTPCVFVSNHPLGGIDGVAVAYEVG
                     KHYPEGIIIPANDLLMYLKNVQDLFIPVNKVGGQAKNLAEALHVAYMSSKQMMMFPSG
                     MVSRKSKGKIEDPEWKKNFIVKAVEYKRNIVPIYIGARNSALFYWVARVRKKLKITLN
                     IEMLLLPHEMFRQRNKELKIVIGKPIQWNVFDDAKTPHKWAQYVKKIVYNLKK"
     gene            5453..6388
                     /locus_tag="PLJ82_05930"
     CDS             5453..6388
                     /locus_tag="PLJ82_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR41081.1"
                     /translation="MEDIIKPIDRALLKAELTSERLLRKTNKGNNEVYLITAKEAPNC
                     MMEIGRLRELSFRTAGGGSGEAYDLDDYDFMDQPYKQLFVWDPDAEEIIGGYRFIHGS
                     QVFLKEDGQPNLVTSEMYHFSPQFIKEYLPYTIELGRSFVQPDYQSSKMGAKSLFALD
                     NLWDGIGALIVSVPDTKYFFGKVTVYPHYNAQARDLVFGFITKFFPDVDKLVYPIDPF
                     HIALSEEQINHVFVNNDYDEDYKILKKEVKDLGTSIPPLVNAYMSLSPAMRSFGFAVY
                     HDFGNLIEVCIMIPIEEMYDEKKKRHVESFFKELE"
     gene            6554..>9173
                     /locus_tag="PLJ82_05935"
     CDS             6554..>9173
                     /locus_tag="PLJ82_05935"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41082.1"
                     /translation="MKQLHQHISSICARCLLLLCFVFLYINTISANFELYILDNSDNV
                     VTQSTFVNAGTNVADEMTTLVAIPSYTGSVYTLSATMLPDTPVVSNYSACQTPGTKTW
                     ASLVTATTGTLVWYADATSTTPIVTPSDFDKDLPQTTSYWVAQESGGIESNRVQVTAT
                     VFAMPPVNAAGEDRTICVGETTLLGVPSQPGFNYSWTPTLQLDVANIAQPTTIALNSE
                     RPFTLTVTNQQNPSCNATSSVTVSIRQRPVITMDKTGVSICAGTTATFGNTNNLPNVS
                     YEWSPNGFLQDPFDGRFVTTQPLTSNTTFTHTVQWNDIPYCPATATVNVTVVANPIAN
                     AGADQNVCYGNDANIGSSLSTGQSFSWEPTGLIDGSTTFKTARTKAMEQTVEFTLTAK
                     TVFPLECTASDKVIVTVIPNPIAFSVTADKTAYCEGDDITDNIVRLEETEADVYYAIF
                     KDGIKLDAFPWRQGDGLAMEWGSLENGIYTVRGRKEAPNNSCAMNMNGTVTIVRKPRP
                     TATITLLNSIACPGETANIKVEFTGTPPYSFKMEINGNEHDYNSATNTFTLSHLLTGA
                     ATFAITEINSDGCSVTYDPGDEPTLVIDIDDVDDFEIIMTPNREKFCPGDVVTLSVVH
                     NNPNSDYLWSTGETTRSIEVSYGANFELTVRTEDGCQYSRQKEVDFGAQDQLQVIGLK
                     PVPTYCSSDGNVTLVGLPDGGAFSGVGIVGNDNIFRPSSVTQSTVGSITYTYSDGFCT
                     KDTIIQNVIVNVPPQVDWEMIPDNAPFASSYNICIPPGEVAVVNLKGSPANTNGDWSL
                     RTDDGSIPSQTTIQIVNKQEGTARLNNVAAGSYYIQYMIKDTYGCNSEKTKMLTVIDQ
                     KQGPLDFGVFDIRPNPM"
BASE COUNT         2713 a         1580 c         1657 g         3123 t
ORIGIN      
        1 tgtatgctaa gagcatcggg cgtggtgtgt gtacttggta tttgctcttt gtgaacataa
       61 ttacatactg tagaaagtaa tccagaaaca tagcctaatt gttcaaatag attatatagc
      121 aaagtggcta tagttgtttt tccattggta cctgttacac ctacaagggt tagtttttgt
      181 gaaggattat cataccaatt agctgccata ttgcccatag ctacagcact atcagcaact
      241 tgtaagtatg caatatcgct atgaatggat gttggtagtt gttcgcaaac aatagctgtg
      301 gctcctaatt caatagcatc tgcaatgtat tgatgcccat ctacagctga ccctttcgta
      361 gcaaaaaata ggtatccttg ttgaacttgt ctcgaatcga aacacaatcc atttatttgt
      421 ctatcagaag tgtagtgcaa acgtctaata actgctatgt tttttactaa ttcgtcaagc
      481 gttttcattt tctcattttt agtttaatgt aagtgttacg ggcatacctt gtacgatgtg
      541 ttcgttttct ggtattgttt gctctactac ttttccaaaa ccaataattg ttacttttag
      601 tcccattttt tctactatat acacagcatc tttagcactc ataccaatag tatttggcat
      661 agtgtgctcc ctcataacaa aaggattaaa cgaggctatt tgtgtagagt cattcacaat
      721 gtattctccc cattgcatag ttttatctgc cattttaatt ggctttgcca tggttttaag
      781 tacttcatta atgtgtggta cgtatccttt ttttatgatt gctaaagaat ccgttacttc
      841 cgaatgagcc caagaaggta tatctcctag tttcattgtg tatacttttt ctgctacatt
      901 tttgaacact gcaccgctca tcccaccagc agatatatgg ggtccctgag ggtcttttat
      961 atatacaatc atcgagtaca tgggtttgtc tgctggaaaa tatcctgcaa atgaaatttg
     1021 aagttttgct ttttgtcctc cttgtttgta ttgtccacca atggctattt gggcagtacc
     1081 cgttttccct gcaattggaa acaatttcga ttttattgct tttccagttc cttttgttac
     1141 tacaccctct aacatcgatt gtattttgcc taaagtagca tcagagcata ttttaggatt
     1201 tataataatg ggttcatatt tttctactgt aataccgtgt tgtcttattt ctttagtaaa
     1261 atatggtttc atcattttcc cattgtttgc aaatgcatta tagtacataa gtgtatagat
     1321 tggaggaatc tgtatttcat agccaattga catccaagga agtgttgtat atgaccaaga
     1381 tttcatactt gggtctttaa tttttacgtg tccgtgtcca ggtattccta aatctcctgc
     1441 ctgatgtatt ttggtgcgtt ttagtaaatc aataaagcgt tgtggattat gggcataatg
     1501 atcgtatata atgctggatg tccctacatt agatgattga tacaatattt ttggcatact
     1561 cattacacca aatccccctt tgttggcatt ccagtctcgc atggttcttt tcccaaattg
     1621 ttttaatcca ttgcctgtat ttacagtaac agtagtatct accacgccat cttctagtgc
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgcttacaa cgtcataccc
     1801 attcacagca tctttcacca caatatcaac aaattttcca gccatttttc tgcgatagta
     1861 gaatccttct tccccttgca aaagtttgtt gtattgtttc tctaagccgc tactacctcc
     1921 tttttcttct tccccataga tgtctccaat agtacgtgag gctagttctc caaaaggttt
     1981 gatgcgggtt actcgttttt ttgtataaaa acctccacca tatcttcctt tttgcaaaat
     2041 tgggaacgtt tttacttcag ctaaatcaat atatgatatg ggtttaggat acaacagata
     2101 ttctctttta cgtttgtttg ttttgtcgta aaaagtgttt aaaaattgtt tctcatattc
     2161 ccatgctgtt ttatctttaa atttttttgc taatgctact gataaacgtc taacgtcttg
     2221 tttaaattct gcttcattct ttgtgctttt ttgcaccaaa gcatcagcca ttaaatccat
     2281 atacaaataa tacgtaggaa ttgagctcgc taaaaggcgt ccatcactcg ctaaaatatt
     2341 acctctgttt ggcttaattg gtcgcaatgc agattgttgg ctagctgtta gtgcccgtaa
     2401 tttttctcct tccacatatt gtagatgaac aattctaaat ataactcctc caaatgcaat
     2461 tagaataaaa atgtagataa ttgcaaaacg aagcataatc tcttttttta ccgtattttt
     2521 acgtataaac aataacacca gcaagctaat gatagcaact actgcaattg caagtaatat
     2581 aaacaaaacc gctttcattg tgttattttg ttgttaattt atacggtgga atacttagtt
     2641 cttttaactc aatagcatgt tggtgtaata atgcttctat ttgtgttctt ctacttatac
     2701 taagcaaatc agcttctact accattgatg tatattttac atcagttaat tgttgattta
     2761 aatcattgat agttgcaatt tgtttttcac attccatacg gttatccatg taaaaaaata
     2821 taaaaaaact aaccaataca attaatttaa tttgttttaa aaaatagctt ttggaaaata
     2881 catttccatc aaaaatatct ttaaccgaag tttttgttaa atcgttaaat tcttcgcttt
     2941 cctttatttt cttttttaat gtttttattt ttgccatact gctatttttt gcttgctatg
     3001 cgtagttttg cacttcttgc tcgtgaattg cttgctattt cttcggtcgt tggaataatt
     3061 actttgttgt ttatcaggct aaatggcgtt tggatattcc caaaaaagtc tttgtttata
     3121 atcccttcca tgttgcctgt ttttaaataa tttttcacca atcgatcttc taatgaatga
     3181 taggtaatgc aaacgaaacg accttctttc tttaaaactt ttgcactact cagtaacatt
     3241 ttttttaatg catccatttc gttgttaacg gctattcgta atgcctgaaa tacttgtgcc
     3301 aattgttttt tttccttttc ttttcctatc aaaggttgta ttatgccgac aaatccaaaa
     3361 atggtttcaa attgttcgtt ttttcgttgc tctactattt ttctcgctat ttttcttgcc
     3421 agaggtaatt ctccataata aaagaatata tccgcaagtt gttgttctgt gtatgtgttt
     3481 aaaatttctt ttgctgtaag ttttgcagct tgattcatgc gcatatctaa tattccatca
     3541 aaacggaatg aaaaaccccg ttctgcatca tcaaaatgat gagaagatac ccctaaatca
     3601 gctaatattc cgtccacttc gttaatccca tagtattgca taaaattttc taagtagcaa
     3661 aagttgcttt gtacaaacgt aaatcggcta tcatttactg cattcatttc tgcatcctta
     3721 tcttggtcaa atccaaatac ttttgcatcg ctatgtagtt gactgagtaa aaaacgagaa
     3781 tgaccacctc caccaaacgt taaatcaaca tatacccccg atttgttagt gagtagacct
     3841 tcaatacttt cttttaaaag aacgggagtg tgatacgttg tcattctata ttttcgtttt
     3901 ttttatttgt taacccttat tatatatttg cgattattgt atatatatgt gcgaaatatt
     3961 gtctcttcgc aatttcttaa tctttcgtag gtttgccaat attcatcaaa ttttctattt
     4021 caagcgcaaa attatccgca tcaataaagg tattttcaac tttattaata ggccaaattt
     4081 caatgacggt atctgctcca acaaaacgaa tatctttgtc aatttgtaag gcatcaatat
     4141 accgttttgg aataagcatt cgcccgtttt cttctaattc aatgcgatcg gcttcggcta
     4201 caaactgtcg aaatatttgc tcttcacgtc tgttccatct gcttaagttg tttcgcaaca
     4261 cagtaataat atcattccaa gcttgttctg tatacaatac caagcaatca tgaaacaatc
     4321 ctttgcgtaa aatgaatttg gtttcatctt gcaattgtct tctgaattgg gctggaacaa
     4381 aggctctgcc ttttgcgtca acttttgctt ctatatttcc aattaatgat gtcatatctt
     4441 gctcttcttg aagtgtaaaa ttaatacttt ttacgacatt ttgcaacttt ttacgacaca
     4501 attccccaca aagttattaa caggctttat ttttagattg ttataaaaaa attgtgtatt
     4561 caatctttgc gtggaaaaaa aaatgtatat ttgtcggcat tataatgaga atgggtagta
     4621 tgttggacat gagtcagcca gtgcaaatag atattgcatc aatattaaaa ggaaagactg
     4681 gggggaagaa accttcttca atgttggttt atttgttgtc taagttattg catgtgaagg
     4741 aaataaatca ttttttaact ttagctaagg ataaaaaaaa tttggacttt tttcaggcat
     4801 taatagagta tctcaatgtg cgtgttttag tttcaggagt cgataatttg cctctatcgg
     4861 gtacgccttg tgtgtttgtg tccaatcatc cattaggtgg tatagatggc gtagcagttg
     4921 cgtatgaggt aggtaagcat tatcctgagg ggataattat cccagccaat gatttgttga
     4981 tgtatttaaa aaatgtacaa gatttgttta tcccggttaa taaggtggga gggcaggcaa
     5041 aaaatttggc agaagcctta catgtggcat atatgtcatc taagcaaatg atgatgtttc
     5101 cgtcaggaat ggtttcgcgt aaatcgaaag gaaaaataga agaccctgag tggaaaaaaa
     5161 attttattgt aaaagcagta gagtataaac gtaacatagt ccctatatat ataggagcac
     5221 gtaattcagc tttgttttat tgggtagcac gtgtgcgtaa aaaattgaaa ataaccctta
     5281 atatagaaat gctattgttg ccacatgaaa tgtttcgcca gagaaataaa gagttgaaaa
     5341 ttgttatagg taaacctatt caatggaatg tgtttgatga tgcaaaaaca cctcataagt
     5401 gggcacaata tgtaaaaaaa atagtgtata atttaaaaaa ataaccaaaa ctgtggaaga
     5461 tattataaag ccaatagaca gagctttact aaaagccgaa ttaacctccg agagattgtt
     5521 gagaaaaacc aacaaaggga ataacgaggt ttatttaata acggcaaaag aggctccaaa
     5581 ttgcatgatg gagataggtc gtttgcgtga gttgtcgttt cgtacagctg gtggaggtag
     5641 tggagaggct tatgatttgg atgattatga ttttatggat caaccctata agcaattgtt
     5701 tgtgtgggat ccagacgcag aagaaattat aggtggttat cgttttatac atggttctca
     5761 agtgttttta aaagaagatg gtcaacccaa tttggttacc tccgaaatgt accatttttc
     5821 acctcaattt atcaaagagt atttgcccta tactatcgaa ctgggtcgtt cgtttgtgca
     5881 gccagattac caatcgagca aaatgggtgc aaaaagcttg tttgcgttgg ataatttgtg
     5941 ggatggaatc ggggctttaa ttgtttccgt gcccgatact aagtatttct ttggcaaggt
     6001 tactgtgtat ccgcattaca atgcacaagc acgtgatttg gtttttgggt ttatcacaaa
     6061 attctttcca gatgtcgata aactagtgta tccaattgat ccgtttcata ttgctctttc
     6121 tgaggaacaa atcaaccatg tatttgttaa caatgattat gatgaggatt ataagatttt
     6181 aaagaaagaa gtaaaagatt tgggcacaag cattccccca ttggtaaatg cgtatatgag
     6241 cttgtcgcca gctatgcgtt cgtttggttt tgccgtttac catgattttg gcaatttgat
     6301 agaggtatgt ataatgattc ctatcgaaga gatgtatgat gaaaagaaaa aaagacatgt
     6361 tgagtctttt tttaaggaac tggaataatc aaagtttgaa ataattagtt accaaaatag
     6421 cgaaaatgtt acgtttcgct atttttttgt taaataatca cgttaaacat aaaaatatat
     6481 gaaaaaattc agttaattat ttatatattt gtagtctctc tttagaagga aaaaccttaa
     6541 tattgaaaca gctatgaaac agttacacca acatatttcc agtatctgtg caagatgctt
     6601 gttgttattg tgttttgtat ttttatatat caacaccatt tcggcaaatt ttgaattgta
     6661 tattcttgac aattctgata acgtagtaac acaatcaact tttgtaaatg caggcaccaa
     6721 tgtggctgat gaaatgacaa ccttggttgc gatcccatct tatacaggtt ctgtttatac
     6781 actatcagct accatgttgc ccgatactcc tgtggtaagc aattatagtg cttgtcaaac
     6841 acccggtacg aagacatggg caagtttggt aacagctaca acaggcacct tggtgtggta
     6901 tgctgatgct acttctacaa cgcctattgt aacaccctca gattttgata aagatcttcc
     6961 tcaaaccacc tcttattggg tggcacaaga aagtggagga atagaaagta acagggtaca
     7021 agtaacggca actgtatttg caatgccacc agtaaatgcg gcaggagagg atagaacgat
     7081 ttgtgttggt gaaactactc tattgggtgt tccatcacag cctggtttta attatagttg
     7141 gacgccaaca ctccaattgg atgttgcaaa tatagcccaa ccaactacca ttgcgttgaa
     7201 ctcagaaaga ccatttacat taactgttac caatcagcaa aaccctagtt gtaacgctac
     7261 atcgagtgtt acggtatcta tacgacaacg accagtgata acgatggata aaacgggtgt
     7321 gagtatttgt gctggtacaa ctgctacatt tggcaataca aataatttac cgaatgtaag
     7381 ctatgaatgg agtccaaatg gatttttgca agacccattt gatgggcgat ttgttactac
     7441 ccaaccatta acatcaaata ccacgtttac gcatacagta cagtggaacg atatccctta
     7501 ttgcccggct actgcgactg ttaacgtaac tgttgtggca aatccaattg ccaatgcagg
     7561 tgcagatcaa aatgtgtgtt atggtaatga tgctaatatt ggtagctcac tcagtactgg
     7621 acaatccttt tcttgggagc caacgggttt gattgacggt agtaccactt ttaaaacagc
     7681 tcgaaccaaa gcaatggaac aaactgttga gtttacgtta actgcaaaaa ccgtttttcc
     7741 attagaatgt acagccagcg ataaagtaat agttactgtt ataccaaacc caatagcttt
     7801 ttccgtaaca gctgataaaa ctgcatattg tgaaggagat gatataacgg ataatattgt
     7861 gcgtttagaa gaaacggagg ccgatgtata ttatgcaata tttaaagatg gaataaaact
     7921 tgacgctttt ccatggcgac agggagacgg cttagcgatg gaatggggaa gccttgaaaa
     7981 tggtatctat actgtaagag ggagaaaaga agctccaaat aactcttgtg ctatgaatat
     8041 gaatggtacg gtaacgattg ttcgtaaacc tagacctacg gctactatta ccttactcaa
     8101 tagtatcgct tgtcctggtg aaactgctaa tataaaggtt gaatttacag gtacacctcc
     8161 ttattccttt aaaatggaaa taaatggaaa cgaacatgat tataattcag ctaccaatac
     8221 atttacgctt tctcatttat taacaggtgc tgcgacattt gcaattaccg aaattaattc
     8281 agacggttgt agcgtaacgt atgatcctgg tgatgagcca accttggtta ttgatattga
     8341 tgatgtagat gattttgaaa ttattatgac tcctaataga gagaaattct gtccaggaga
     8401 tgttgtaaca ttatctgtgg tacacaataa tccaaattct gactatttgt ggagtacggg
     8461 agaaaccact cggagcatcg aagttagtta tggtgcaaat ttcgagttga cggtgagaac
     8521 ggaagatggt tgtcagtata gtagacaaaa agaagtcgat tttggagctc aagatcaatt
     8581 acaggtaata ggactgaaac ctgttccaac ttattgctca agcgatggta atgttaccct
     8641 agtcgggtta cctgatggag gagcatttag tggtgtaggt attgttggca acgacaatat
     8701 atttagacca agttcagtta ctcagagtac tgttgggagc ataacgtata cgtattctga
     8761 tggtttttgt accaaagata ctatcattca gaatgtaatt gttaatgtgc caccacaagt
     8821 ggattgggag atgataccag ataatgcccc gtttgcatcc agttataata tttgtattcc
     8881 tccaggagag gttgctgttg taaatctaaa aggttcccct gcaaatacaa atggtgattg
     8941 gtcgctacga acagatgatg gaagtattcc ttctcaaaca actatacaaa ttgtaaataa
     9001 gcaagaaggt actgctcgtc tcaataatgt tgcagcaggt tcgtattata ttcagtatat
     9061 gattaaggat acatatggct gtaattctga aaaaacgaag atgctgactg taatcgacca
     9121 aaaacaagga cctctagatt ttggtgtttt tgatattaga cccaatccta tgt
//
LOCUS       DAOSRP010000078         9124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7251_length_9124_cov_6.579557, whole genome shotgun sequence.
ACCESSION   DAOSRP010000078 DAOSRP010000000
VERSION     DAOSRP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7251_length_9124_cov_6.579557"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLJ82_05940"
     CDS             <1..374
                     /locus_tag="PLJ82_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOR41083.1"
                     /translation="GTMNTADRSLAIVDYALRRRFRFITLEPKFNSRFKDFLSSKGFS
                     KDFTQEMSSKLNLLNAKIKSDKNLGSGFLIGHSFFCNANSENERKWFENIVKFEIRPL
                     LEEYWFDDNEKVDNEINLLLS"
     gene            377..1405
                     /gene="mcrC"
                     /locus_tag="PLJ82_05945"
     CDS             377..1405
                     /gene="mcrC"
                     /locus_tag="PLJ82_05945"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF007277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HOR41084.1"
                     /translation="MQIPIQNIYYLLCYAWNKLEESEIVNVNEIDSTELIDLFAKVLS
                     NSCSRLLKQGLDRYYVEHENVIMGIKGKFNFSATIKQNVLPLSKTACIYDEFDYDILH
                     NQILKTTIGKLLRTKNLDSSLKDELHKIYLKLPPISEIVIHKSMFTQIRLHRNNYHYD
                     FILKVCQIVNENLFIDETKGNYKFKDFTREEKAMARLFEAFVRNFYKVETDFSVSSDS
                     ISWQFESENVEDLEMLPTMLTDITLQGKNHKIIIDTKYYKEAFQTRYDKQKINSNNLY
                     QLFSYLKNQETDSELTINCEGILLYPSIQNDFVYSFKYENHKIRIMSINLNQDWQNIR
                     TDLLKIVA"
     gene            complement(1438..1764)
                     /locus_tag="PLJ82_05950"
     CDS             complement(1438..1764)
                     /locus_tag="PLJ82_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOR41085.1"
                     /translation="MQQADILFLSNEKIMGTEKELIHALATTYHNQVIVVGLGAEGAL
                     LYQKTTNSIQHLPAVFTRPIINTIGAGDALFSCFLHYYAKGEEPTNALHKAITFASYK
                     IGDNGA"
     gene            complement(1737..2156)
                     /locus_tag="PLJ82_05955"
     CDS             complement(1737..2156)
                     /locus_tag="PLJ82_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOR41086.1"
                     /translation="MSNILVSGLINIETTLQCDEFPIEYSPVRYPFFGIQNTISGVGY
                     NVAKALTLLSDNVQFMSIIGKDKEGEWITDELQSLHIDTHYITPSIAQTPQSVILFDK
                     AGKRQIHVDLKDIQETNYPTYTTHTMLILCNRQISYF"
     gene            complement(2191..2769)
                     /locus_tag="PLJ82_05960"
     CDS             complement(2191..2769)
                     /locus_tag="PLJ82_05960"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOR41087.1"
                     /translation="MKKILIINGNPDKNSLSFELATAYKKGADASGAACTLVHLSDLQ
                     FDLNLKFGYRQRTELEPDLVMMQQAIKEADHLVFVYPTWWGTYPTLLKGFIDRVFLPK
                     FAFSYRENSPLWDKLLVGKTARLIVTMDTPTWYYKWIFKQPGHNSMKKGILEFCGIKP
                     VKITNFGSVKTSTEATCKNWLAEVENLGKKVS"
     gene            complement(2779..5196)
                     /gene="lon"
                     /locus_tag="PLJ82_05965"
     CDS             complement(2779..5196)
                     /gene="lon"
                     /locus_tag="PLJ82_05965"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOR41088.1"
                     /translation="MKDNPNKLFIGTNLQNDDSDFIPLIGDEDDDHVVDLSEDKVLPI
                     LALRNMVLFPGVVIPVTIGRPKSLKLIKDVNKRNGFLGTIAQKDNKIEEPELNDLYAV
                     GTVAQVVRVLEMPDNSTTVILQGRQRFAIQELDATEPYLKAKVELLADSETPKHKDFK
                     ALMEAIKDLSLRMIKISSNMPQEIMFAIRNIENPRFFINFISSNLGLSLIDKQSLLEI
                     NNIEQRAMMLLKILTKESQLLEMKAAIQSQAMENIDKQQREYFLQQQMKTIQKELGGN
                     VQDDLINDIKKKAEGKKWDEKVRKIAEKEIAKLETTSTHSSDYSVQLNYVQTLVDLPW
                     NEYTKDNFNLKRAQRVLNRDHFGLDEVKDRIIDHLAVLKLKGDLKSPIICLYGPPGVG
                     KTSLGKSIADALGRKYARISLGGLHDEAEIRGHRRTYIGAMPGRVIQNILKTNSSNPV
                     LVLDEIDKVGRDYKGDPGSALLEVLDPEQNFAFHDNYLDVDYDLSKVLFIATANSLSS
                     ISQPLLDRMELINVTGYIVEEKIEIAMKHLIPKQLENHGIPKDAVSFSKKTVQEIVEK
                     YTRESGVRNLEKQIAKICRRLARKIAMEEKYDTSMPISDLKEYLGTEKYTRDKQSQKE
                     EIGIVTGLAWTSVGGEILEIETSLSKGKGGKLTLTGNLGDVMKESAMLGLEYIRAHAK
                     DFGLNEDLFEETNVHIHVPEGAIPKDGPSAGITMATSLLSAFTKRKVKKHIAMTGEIT
                     LHGKVLPVGGIKEKILAAKRAGITDIILSHENKKNIDEINAIYIKGVTFHYVKDIKEV
                     IQIALLK"
     gene            complement(5349..6368)
                     /gene="pheS"
                     /locus_tag="PLJ82_05970"
     CDS             complement(5349..6368)
                     /gene="pheS"
                     /locus_tag="PLJ82_05970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOR41089.1"
                     /translation="MKEKIEKLLAEIDILSPISKEEVEELRIKYLSKKGEISTLFNDF
                     RNVANEEKKVVGQLLNDLKNKAQDKINELKETFDNQQNNTNDLDLTRSSSPIELGTRH
                     PLSIVKNEIVDIFARIGFTISEGPEIEDDWHVFSALNFPPEHPARDMQDTFFIEKNPD
                     VLLRTHTSSVQTRVMKQAQPPFRVLCPGRVFRNEAISYRAHCFFHQVEGFYVAENVSF
                     ADLKQALLYFAKEMFGEHTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKHTGWV
                     EILGCGMIDPNVLESCGIDSKKYTGYAFGMGIERITNLKFQVKDLRMFSENDVRFLKQ
                     FEAAL"
     gene            6598..7314
                     /locus_tag="PLJ82_05975"
     CDS             6598..7314
                     /locus_tag="PLJ82_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOR41090.1"
                     /translation="MQKIHVITPVKDSIELSLQTIQSVCNSQTSMPFVYTVYNDFSTD
                     ENTQTLQQASQEFGFELINLLSITSTPSPNYLLVLQLAQQKAIDEDATLVIVESDVLV
                     QTNTLQALIDTAQQKTDAGMVASITVDESGNVNYPYLFAKKIKGLVKPTNKHVSFCCT
                     LLSLPYLKAYSFTSLQPDKNWYDVHISRQSRQLGFVNYLLGNHPVVHRPHSSRPWKQL
                     KYSNPIKYYWLKITQQKDKI"
     gene            7314..8801
                     /locus_tag="PLJ82_05980"
     CDS             7314..8801
                     /locus_tag="PLJ82_05980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR41091.1"
                     /translation="MQQVAFLPKVSVVIPIYNMELYLEETILSVLASSYTDYEVLLID
                     DGSTDNSVSIAKRFANAYPHIYFYEQPNQGVSAARNNAIKWSKGDYILPVDADNLIGK
                     EYISEAVNVLETYPNVKVVTCEAEFIGEKTGKWKQLPFSLSLLARKNMIDNCAMYRKS
                     DWQDSGGYCEEILGREDWDFWISMLKNGGDVLRLPIVGLYYRVRSDSKRRKTQNRKKK
                     LIDLLNVRHADFFEKQLHGRLHYNRTYSKLFNLLEKIFGKRTTVIHPTYSQLAPCIER
                     LPLAFLVNNNVIHQGRNTLKQFSENGLDLVVKSYQIPHIINRLSYGFFRSSKAKRAYE
                     YALTLQLHKIGTPQPIAYIEQRFAGLLYQSYFISVRSTCPYTFNTLIQQPSYEYRTLV
                     LQEIGRFTADLHTKGMLHQDYSGGNILFDVQNGNVLLELVDLNRIVFKHTIGIEEGCK
                     NFERLNIDEEALQILATEYAKARNFDVDMCVESVLKMRWHKHKQR"
     gene            8912..>9124
                     /locus_tag="PLJ82_05985"
     CDS             8912..>9124
                     /locus_tag="PLJ82_05985"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR41092.1"
                     /translation="MMDNGSTDETWEIVQQLATEYPQIIPFAKDAQAFQIGLRAVMFD
                     AFKSELTASDWWCIRLDADEFFVENPR"
BASE COUNT         2812 a         1620 c         1663 g         3029 t
ORIGIN      
        1 taggtacaat gaatacagcc gaccgttcat tagctattgt tgactatgct ttacgtaggc
       61 gatttagatt tattacattg gaacctaaat ttaacagcag gtttaaagat ttcctcagtt
      121 ctaaagggtt ttcaaaagat tttacacagg aaatgtcttc aaaacttaat ttacttaatg
      181 caaaaataaa atccgataaa aacttaggct cgggcttttt gattgggcat agcttctttt
      241 gcaacgcaaa ttctgaaaat gaaagaaaat ggtttgagaa tattgtaaaa tttgaaataa
      301 ggccattact ggaagaatac tggtttgatg ataatgaaaa agttgataat gaaataaatc
      361 tacttttatc gtaattatgc aaataccaat tcaaaatatc tattatttgc tatgttatgc
      421 atggaataag cttgaagaaa gtgaaatagt caatgtaaat gaaattgatt caaccgaatt
      481 aattgactta ttcgcgaaag tcctttcaaa tagctgttca cgcttattaa agcaaggttt
      541 agacaggtat tatgttgagc atgaaaatgt gataatgggc attaaaggaa aatttaattt
      601 ctcagcaacg ataaaacaaa atgttcttcc tttaagcaaa acagcatgta tttacgatga
      661 atttgattat gacattttac acaaccaaat tttaaaaacg acaatcggaa aattactaag
      721 gacaaagaat ttagattcat cgttaaaaga tgaattgcat aaaatatatt tgaaattgcc
      781 gcccatttct gaaatcgtta tacataagtc catgtttact caaattaggc tacatcgcaa
      841 taattatcac tatgatttca ttttaaaagt atgccaaatt gtaaatgaga acttatttat
      901 tgacgagacc aaaggaaact ataaattcaa ggattttact cgggaagaaa aggcaatggc
      961 aagattgttt gaagcttttg taagaaactt ttataaggtt gaaacagact tttctgtatc
     1021 aagtgattca atttcatggc agtttgagtc agaaaatgta gaggatttag agatgttgcc
     1081 tacgatgctc acagacatta ccttgcaagg caagaatcat aaaataataa tcgatacaaa
     1141 atattacaaa gaagcatttc aaaccagata tgataagcaa aaaataaact cgaataattt
     1201 atatcaactc ttttcatatt taaaaaatca ggagactgat tctgaactga cgataaattg
     1261 tgaagggata ttactttatc cttcaatcca aaatgatttt gtgtactcat ttaaatatga
     1321 gaaccataaa ataaggatta tgtcgataaa tctaaatcaa gattggcaga acataaggac
     1381 tgatttatta aaaattgtag catgagaacc aatccaacac acaccatccc ttcgctctca
     1441 ggcaccattg tcgccaattt tgtacgaggc aaatgtaatg gctttgtgta aggcgtttgt
     1501 aggttcttct cctttggcgt aatagtgcaa aaaacacgag aacaaagcat cgccagcacc
     1561 tattgtgttg ataatggggc gtgtaaatac ggctggtaga tgttgtatgg agttagtcgt
     1621 tttctgatag agcaatgctc cctcagcacc caaacctact acaattactt ggttgtgata
     1681 ggtagtagct aaagcatgaa tcagttcctt ttctgtgccc ataatttttt cgttgctcaa
     1741 aaataagata tctgcctgtt gcataaaatc aacattgtat gggtcgtgta tgtcggataa
     1801 ttcgtttctt gaatatcctt taaatctaca tgtatttggc gtttaccagc tttgtcgaag
     1861 agtattaccg actgtggtgt ttgagcgatg ctgggtgtaa tgtaatgtgt gtcaatgtgt
     1921 agcgattgta attcgtcggt tatccattcg ccttctttgt cttttccgat aatagacata
     1981 aactgaacat tgtcggatag cagggtaaga gctttagcta cattgtagcc cacgcccgaa
     2041 atggtatttt gaataccaaa aaatgggtaa cgcactggcg aatattcaat cggaaactca
     2101 tcgcattgca aggtcgtttc tatgtttatt aaaccagata caaggatatt gctcatgcct
     2161 tacttcgatt tggttactat acgtaggagc ttaggatacc ttttttccta aattttcgac
     2221 ctctgccaac caatttttac atgttgcttc tgttgaggtt ttcacgctac caaagttggt
     2281 gatttttaca ggtttgatac cacaaaactc caaaatgcct tttttcatgg agttatgtcc
     2341 gggttgttta aaaatccatt tatagtacca agtaggtgta tccatggtta caatcaatcg
     2401 agccgtttta cctaccaata atttatccca tagtggcgag ttttcgcggt acgagaatgc
     2461 aaattttggt aaaaatacac ggtcaataaa ccctttgagc aaggtagggt aggttcccca
     2521 ccaagtagga tatacaaaca ccagatggtc agcctcttta atggcttgtt gcatcattac
     2581 caaatcgggt tctagttcgg tgcgttgacg gtagccaaat ttaaggttca aatcaaattg
     2641 caggtcgctt aaatggacta aggtacaagc tgcaccactg gcgtcagctc cttttttgta
     2701 cgcagtagct aattcaaagc tcaaactatt tttgtcggga ttgccgttga tgattaaaat
     2761 tttcttcata catcccactt attttagtaa agcaatttga attacctctt taatatcttt
     2821 tacataatga aacgtaaccc ctttgatata aatggcatta atttcatcaa tattcttttt
     2881 gttttcgtgc gacaaaataa tatctgtaat accagcacgt ttggcagcaa gaattttttc
     2941 tttaatccca cctacaggca acacttttcc atgtaaggta atttctccag tcatggcaat
     3001 atgttttttt acttttcgtt tggtaaacgc agataacaag ctagtagcca ttgtaatacc
     3061 agcagaaggt ccatctttcg gtatagcacc ttcgggtaca tgaatatgta cgttggtttc
     3121 ttcaaatagg tcttcgttaa gtccaaaatc cttggcatga gcacggatat attccaaccc
     3181 aagcatggca gattctttca tcacgtcgcc taaattaccc gtaagggtta gtttgccacc
     3241 tttgcctttg ctaagactgg tttcaatctc taaaatttcg ccacctactg atgtccaagc
     3301 caaaccagta acaataccaa tttcttcttt ctgactttgc ttatctctgg tatatttttc
     3361 tgttcctaag tattctttaa ggtcgctaat tggcatagat gtgtcgtatt tttcttccat
     3421 agcaattttg cgagccagtc tgcgacaaat ttttgcaatt tgtttttcta aattacgtac
     3481 gcccgattcg cgtgtgtatt tctctacaat ttcttgtacc gtttttttag aaaatgaaac
     3541 agcgtctttt ggaataccat gattctctaa ttgtttagga atcaggtgtt tcattgctat
     3601 ttcaattttt tcttctacaa tatagccagt tacgttaatc aactccattc tgtcgagcaa
     3661 tggttgcgaa atactactca agctattggc tgtggcaata aataatactt tagacaaatc
     3721 gtaatctaca tctaaataat tatcgtggaa ggcaaagttt tgttctgggt cgagcacttc
     3781 gagcaaagca gagcctgggt cgcctttgta atcgcgacca actttatcta tttcgtctaa
     3841 taccaaaaca ggattagatg agtttgtttt taaaatattt tggataacac gtccaggcat
     3901 agctccaatg tacgtacgac ggtgtccacg aatttcagct tcatcgtgca atccacccaa
     3961 cgatatacgt gcatattttc tgcccaatgc atcagcaatg gattttccta acgatgtttt
     4021 tccaacccct ggaggtccat acaagcaaat gataggcgat ttcaaatctc cttttaattt
     4081 aagtacagcc aaatggtcaa taattctgtc ctttacttcg tctaatccaa aatggtctct
     4141 attcagcaca cgttgggcac gtttcaggtt aaaattatct ttggtatatt cattccaagg
     4201 caaatcgact aaggtttgca cataattgag ttgtacagaa taatcgctcg aatgggtact
     4261 agttgtttct agtttcgcaa tttccttttc ggcaatttta cgtacttttt catcccattt
     4321 ttttccttcg gcttttttct taatatcgtt tatcaagtcg tcttgcacat tgccacccaa
     4381 ttctttttga atggttttca tttgttgctg caaaaaatat tcgcgttgtt gcttgtcgat
     4441 attttccatc gcttgcgatt ggatagcggc tttcatctcg agcagttgcg attctttggt
     4501 caagattttt agcaacatca tagcacgttg ctctatgtta ttgatttcca ataagctttg
     4561 tttgtcaata agcgagagtc cgagattaga gctaataaag ttgataaaaa aacgaggatt
     4621 ttctatgttg cggatagcaa acataatttc ctgaggcata ttgctagata tttttatcat
     4681 gcgtagcgat aggtctttta tcgcctccat caatgcctta aaatccttgt gtttaggggt
     4741 ttccgaatcg gctaaaagtt ctactttggc tttaagataa ggttcggtag catctaattc
     4801 ttgaatggca aaacgctgac gaccttgtaa aataaccgtg gttgaattat cgggcatttc
     4861 tagtactcgt actacctgag ccacagtacc tacagcgtat aagtcattta gttcaggctc
     4921 ttctatctta ttgtcttttt gtgcaatagt acccaaaaaa ccattccgtt tgtttacgtc
     4981 ttttatcagt tttagcgatt ttggacgacc aattgtaacg ggaattacta ctccaggaaa
     5041 taaaaccata ttacgcagag ccaaaatggg caacaccttg tcttcgctca aatctactac
     5101 atgatcgtca tcttcatcgc caattaaggg aataaaatcg ctatcatcgt tttgtaaatt
     5161 tgttcctata aaaagtttat ttggattatc tttcatgttc gtttttacta ttattggcta
     5221 cccatagcct ttattttttt gatgttttat ctcgtctcta aagcaataca atactatact
     5281 aatcaaaatg tatgccaata caaatttctg ccaaatgaga gaaaaatata tgtcagagaa
     5341 aaatatagtt atagagctgc ctcaaattgt tttaggaaac gcacgtcatt ttcggagaac
     5401 atccgtaaat ctttcacttg aaatttgagg tttgtaattc gctcaatacc cataccaaaa
     5461 gcataaccag tgtatttttt actatcaata ccacagcttt cgagtacatt tgggtcaatc
     5521 ataccacaac ctaaaatttc tacccaacca gtgtgtttgc agaaaggaca acctttgcct
     5581 ccgcataagt tacaggatat atccatttca gccgaaggtt cggtaaaagg gaaatacgat
     5641 ggacgaagac gaatttgtgt gtgttcgccg aacatttctt tcgcaaaata caatagagct
     5701 tgttttaaat cagcaaaaga cacattttca gccacataaa atccttctac ttgatgaaag
     5761 aaacaatggg cacggtacga aatggcttcg ttacgaaata cccttcctgg acacaatact
     5821 ctgaaaggtg gttgtgcttg tttcatcaca cgagtttgta ccgatgaggt atgcgtacgc
     5881 aacaatacgt caggattttt ttcgataaag aatgtatctt gcatatcgcg tgcaggatgt
     5941 tcgggcggaa aattgagggc agaaaataca tgccagtcat cttcaatttc tggaccctca
     6001 gaaatagtaa aaccgatacg tgcaaaaata tctacgattt cgtttttaac gatagataaa
     6061 ggatgacgtg tacctaattc aataggagat gaactccgag ttaggtctag gtcgttggtg
     6121 ttattttgtt ggttatcaaa tgtttcttta agctcattga ttttatcttg tgctttattt
     6181 tttaggtcgt tgagtaattg tcccactact tttttttctt cgttggcaac gtttctaaaa
     6241 tcattgaata aagtacttat ttctcctttt ttacttaagt atttgatgcg aagttcttct
     6301 acttcctctt tgcttatagg gcttagaatg tcaatctcag ctaatagttt ttcaattttt
     6361 tctttcatga gtctatcaat aatatcgata acgaatgagt actatgtttg tttaaaatat
     6421 aagtggcata taccacaaaa gaaccacaaa aatacattat ttatttggtt ttttttgact
     6481 ttttgctgtt tttttgctct tctagtcatt ctgttttact tttctataaa aaaatatgca
     6541 cttttgcatc actaagcact tttgcttctc tctatatata attttgatgt aatccagatg
     6601 caaaaaattc atgttattac acccgttaaa gattcgattg aattaagttt acaaaccata
     6661 cagtctgttt gtaattcgca aacttctatg ccttttgtat atacggtgta caacgatttt
     6721 agcacagacg aaaatacaca aacgttacaa caagcatcgc aagagtttgg ttttgagttg
     6781 attaatttat tgtcaattac ttctactcct tctccaaatt atttgttggt gttgcaactc
     6841 gctcagcaaa aagccattga tgaagatgcc acattggtta ttgtagagtc agatgtgctg
     6901 gtgcaaacga acacactaca agctttaata gacactgcac aacaaaaaac ggatgctgga
     6961 atggtggctt ctattaccgt ggacgaaagt ggaaatgtaa attatccata cttgtttgca
     7021 aaaaaaataa aagggctagt aaaaccgacc aataagcatg tgagtttttg ttgtacactc
     7081 ttgtcgttac cctatttaaa agcttatagc tttacctcat tacaaccaga taaaaactgg
     7141 tatgatgtgc atatttctcg tcaatcacgc caattgggtt ttgttaatta tttattggga
     7201 aaccatcctg tggtacatcg cccacatagt agtcgcccgt ggaaacaatt gaaatattcg
     7261 aatccaatta agtattactg gctaaaaatt acccaacaaa aagacaaaat ttgatgcaac
     7321 aagtagcctt tttacctaaa gtttcggtcg taattcctat ttacaacatg gaactatacc
     7381 tagaagaaac catcttgtcg gtacttgcgt catcttatac agattatgag gtgttactaa
     7441 tagacgatgg ttctactgat aattcggttt ctattgccaa acgttttgcc aacgcatacc
     7501 ctcatattta tttttacgaa caacctaacc aaggagtatc ggcagctaga aataatgcga
     7561 ttaagtggag caaaggcgat tatattttgc ccgttgatgc tgataatctg attggtaaag
     7621 agtatatttc agaagcggta aatgtattag aaacttaccc caatgtaaaa gtagtaacgt
     7681 gcgaagctga gtttataggc gaaaaaacgg gaaaatggaa acaattaccc tttagcttgt
     7741 cattgcttgc acgtaagaat atgattgaca actgtgccat gtatcgtaaa agtgattggc
     7801 aagattctgg tggttattgc gaggagattt tgggaaggga agattgggat ttttggattt
     7861 cgatgctcaa aaatggggga gatgtgttgc gtttgcccat tgtgggtttg tattaccgtg
     7921 tacgttctga ttctaaaaga agaaaaacgc aaaatagaaa aaagaaatta attgatttgt
     7981 tgaatgtgcg tcatgcggat ttttttgaga aacaattgca cggtcggtta cattataatc
     8041 gcacctattc aaagttgttt aatttgttgg agaaaatctt tggtaagaga acaaccgtta
     8101 tacatcctac ctattcgcaa ctggctccat gtattgaacg actgccatta gcgtttttag
     8161 taaataataa tgttatccat caaggcagaa ataccttgaa acaattttct gaaaatggct
     8221 tggatttggt cgtgaaatca tatcaaattc cacatataat caatcgatta tcgtatggat
     8281 tttttcgttc atcaaaagcc aagcgagcct acgaatatgc tcttacattg caactgcata
     8341 aaattggcac accacaacct attgcctata tcgagcaacg ctttgcaggc ttactctatc
     8401 agagttattt tataagtgtg cgttctacct gtccgtacac cttcaataca ttaattcagc
     8461 aaccttcata cgaatatcgt acattggttt tgcaagaaat aggcagattt acggctgatt
     8521 tacatacaaa aggaatgttg catcaagatt attcgggagg taatatttta tttgatgtac
     8581 aaaatggcaa tgtattactt gaattagtcg atttgaatag aattgtgttt aagcacacga
     8641 ttggaattga agaaggttgc aaaaattttg aacgtttaaa tattgacgaa gaagctttgc
     8701 agatactggc tactgaatat gccaaagcta gaaactttga tgtggatatg tgtgtggaaa
     8761 gtgtactaaa gatgcgttgg cataaacata aacaacgtta agttaaacag ttagtttaga
     8821 tgaaaatata cagtttactc cttgttaaga atgaagcaga tatcattgca tctatttaaa
     8881 agccgctgcc tagtggagcg ataaagtgat agtgatggat aatggtagta ctgatgaaac
     8941 ctgggaaatt gtacagcagt tagcaacaga atatccgcaa attataccct ttgccaaaga
     9001 tgctcaagca tttcagatag gattgagagc ggtaatgttt gatgctttta agtccgaatt
     9061 aacagcctct gattggtggt gtattcgctt agatgctgat gagttttttg tggagaatcc
     9121 aaga
//
LOCUS       DAOSRP010000079         9080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7296_length_9080_cov_5.017839, whole genome shotgun sequence.
ACCESSION   DAOSRP010000079 DAOSRP010000000
VERSION     DAOSRP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7296_length_9080_cov_5.017839"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..843
                     /locus_tag="PLJ82_05990"
     CDS             226..843
                     /locus_tag="PLJ82_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOR41093.1"
                     /translation="MNLAKPMQDDSYNSIAQLSEGMYKEKGSKFLSFAIPVTSVEEAK
                     DILKDYRKQYYDARHICYAYVIGYPNSVVRSSDDGEPSGTAGKPILAQIQAKQLSDVM
                     VVVVRYFGGVLLGTGGLVVAYREATVDALNNTTVYTKIVEQALTIQCAYPQLNEVMKI
                     IKDNNLTIVSQTLEISCSLKVQIRLSNFETITERLKQVHGLQVIE"
     gene            complement(914..1351)
                     /locus_tag="PLJ82_05995"
     CDS             complement(914..1351)
                     /locus_tag="PLJ82_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR41094.1"
                     /translation="MSLVKFSNNYPTIFDRFFDNNFYDWTSENYSTTNTTVPSANIKE
                     SAEKFEIHIAAPSFAKDDFKIEVNNNVLTVCSEKKVENETKEGERFTRKEFSYQSFCR
                     SFTLPISADTEKISANYENGILAVDIPKREEAKPKPSKIIEIK"
     gene            complement(1494..1967)
                     /locus_tag="PLJ82_06000"
     CDS             complement(1494..1967)
                     /locus_tag="PLJ82_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOR41095.1"
                     /translation="MKTENNSSCCTSSSEYSQQIGKLALRLLVGGFMLTHGLPKLQNF
                     EFLSTVFPDPLGVGSSLSLGMIVFAEVVCSILLMLGLFTRLSTIPLIIGMGVAAFFIH
                     ANDPFSGKEMALLYMGIYVIIALIGAGKYSADYLLRNQYAKICSSYCKPKAENTK"
     gene            complement(1991..2938)
                     /locus_tag="PLJ82_06005"
     CDS             complement(1991..2938)
                     /locus_tag="PLJ82_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOR41096.1"
                     /translation="MKKHLLCLIVLFYFFISTTYSQENTLPRVSSGTIIRHVDFTSNY
                     ISARNVDVWLPDNYSPNQRYKVLYMHDGQMLFDANTTWNKQEWGVDETITRLIANDSI
                     LSTIVVGIWNSGRTRHSDYFPQKPFERLPVSTQDSLILLGRRQNNVSLFAERVQSDNY
                     LRFIVEELKPFIDKTYATLTEPEHTFIAGSSMGGLISMYAVCEYPEVFGGAACLSTHW
                     IGSFQTEGNPIPAQFFAYLKENVPSPDTHKFYFDFGTETLDVHYEPYQQQVDAIMQAK
                     GYNSTNWITRKFEGKDHSENAWRERLHIPLTFLLPIQFN"
     gene            complement(2984..3361)
                     /gene="folK"
                     /locus_tag="PLJ82_06010"
     CDS             complement(2984..3361)
                     /gene="folK"
                     /locus_tag="PLJ82_06010"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOR41097.1"
                     /translation="MNTHHYILLLGSNFLAHKHLSIAIELLQQEFGIYRTSHEMETKA
                     IDMPNDYPFVNKAVCIQTRLEPTELIQKIKEMEKQLGRSDEKTLQGIIPIDIDVILCD
                     KKVLHSDYETKNYVKKLVEEVSV"
     gene            complement(3364..4821)
                     /locus_tag="PLJ82_06015"
     CDS             complement(3364..4821)
                     /locus_tag="PLJ82_06015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOR41098.1"
                     /translation="MKISAFIPYENDEQVAQTIASLQGSNLIGKIFLLATKQASTSTK
                     FDAQTLTIDTLTSTATMRLIADNCTNDFALIYLKYTSLYMGAYAIERLAQVANDTQAG
                     MVYTDYYQQTTTGTAKVPVNDYQIGSVRDDFNFGSLLFFATKVLQSSVASCSKDFAHA
                     GLYQLRLTVSEQSQLVHLNEFLYTEIEQDTRKSGEKQFDYVDPKNRAVQIEMEQVCTQ
                     HLKNIGAYLTPIFPAVNLHQDLFAYEASVIIPVKNRVKTIEDAIVSVLKQETNFPFNL
                     LIIDNHSTDGTTELIQSYAKKNASIVHIIPQRNDLGIGGCWNEGVHHEKCGKFAVQLD
                     SDDLYENATTLQQIVNAFYEQNCAMVIGSYSMTDFALQPIPPGLIDHKEWTPENGRNN
                     ALRINGLGAPRAFYTPVLREIKVPNTSYGEDYALGLQISRNYQIGRIYNSVYLCRRWN
                     ENSDAALDIEKMNAHNAYKDKLRSIEMMARIAMNK"
     gene            complement(4844..7798)
                     /locus_tag="PLJ82_06020"
     CDS             complement(4844..7798)
                     /locus_tag="PLJ82_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HOR41099.1"
                     /translation="MMQAYFRWHYIILFVSCWIFSTSMLAQAKYKLQTIRYTPEITAK
                     KTVYSVSKQTLKNNTLTLWVENDVANIPFREESIQAFYDSVYAQLPATYRENQLEIIC
                     NNRTLDELVPNAYRKTIAIDNARKFTPPHITPVIQNVSKPYSPTQGLLHRHIALWNSH
                     GWYYSAITDKWDWQRGRLFGTVEDMLTTAIVLPYVLPMLENAGANVFIPRERDTQTNE
                     VIIDNDDDATSASITQTSSWEKKENGFKNKKLLQGRDNPFTMGSFLQTPTYEEATDSI
                     EWKIKVPENGMYYVSIAYKSVSNSTESALYSLHHAGGTTRFSVNQQLGGGTWIYLGNY
                     YFIANKLYSLVLTNSGKEGYVVTADVVRVGGGMGNIDRQTTESIVEKKLFPPFIDEDA
                     ISQPPLISTPRARFTEAARYYLQWAGMPDSILINQRKDVISDYQDDIFGRANWVNYLM
                     GGSAMYPSYKGLGIPIDACIALHTDAGKMSGDSIVGTLGIAMTTDDKGMYPANYSRIA
                     ARDFTDMVQTELVHDIRATFPVNWTRREIWDKSYIEARIPKTPTTIIELLSHQNFNDM
                     RYAHCPEFRFVTARAIYKGIVKFLASQYNQPYTIQPLPITHFVAEVLQDDSVHLSWRK
                     TTDPLEPTANPDGYIVYTRKNDGGFDNGIVSKTESITLPIEKGTIYSYKIAALNNGGE
                     SFASEILSVYKAEIEKGKAWVVNAFDRVAAPSSFETDSTAGFTHATDRGVPYLYDLSF
                     TGNQIEYNKSVPFYDNDYTGFGASQANYDGQVIAGNTFDNCYIHGRALAANNYSFSSA
                     SRDGITVLSPSFTGYDVLAIILGNQQTSKTFTRTFTVYPTDLQTLITNYTKQNGKIIV
                     SGAYIASDITRKKDKSEMQFAQNILKYKLRMPQASVNATMVDVFSAKKRQREYNCFTQ
                     PNPYQYAVQQVDAIQPTDKNSSVLLRYTENNTAAAIGYKGKYKTIVCGFPFETLTSTE
                     QQNELMRLFVNY"
     gene            7942..8781
                     /locus_tag="PLJ82_06025"
     CDS             7942..8781
                     /locus_tag="PLJ82_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOR41100.1"
                     /translation="MKLLADSGSTKTAWVVLNENGSNAVEYITDGLNPFFQTVQSVQQ
                     TIQNQLLPHIDANSISEIYFYGAGCAFPEKNAIIEEGLRTVFASATIHVASDLLGAAR
                     GLCGMQDGIACIMGTGSNSCMYQDSKIVHNVSPLGYILGDEGSGAVLGKLLVGDYLKN
                     QMPTHLASAFLATYQLTPAIILDRVYKQAFPNRFLASFSPFLSAHKHEPYVQALLHRS
                     FTDFFVRNIQQYDYQHHTAHFTGSIAYYFQDILQQVAQERGVAVGVIEQTPMPGLVRF
                     HGV"
     gene            complement(8769..>9080)
                     /locus_tag="PLJ82_06030"
     CDS             complement(8769..>9080)
                     /locus_tag="PLJ82_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOR41101.1"
                     /translation="GLTVLCFEIGNKNIPPTVTFLVLPTFAQQSTSEIVNLSLEDCIQ
                     LAYKKNNNLQITLLNESANQEIYKQSKLERLPNLNASLGETFTHSDSNGSNWNYDTYT
                     P"
BASE COUNT         2622 a         1597 c         1780 g         3081 t
ORIGIN      
        1 cacatctccc aaacccaatc gaataacatg actatttgga tgaatgacat tgaaagcatt
       61 gatgcgtttt tctatttcgg tgaaaaagaa attttcagga agtttcagat aattttcgtt
      121 gattaatacc atgatttgtt gtttacatca gctaatgtta tgcgaagata aaaaaacaaa
      181 atcaaaaacg agctattttt aacttaaaag tgtaattttg tctgtatgaa tttagccaaa
      241 ccgatgcaag acgatagtta caatagtatt gctcaattat cagaaggtat gtataaagag
      301 aagggtagta aatttttgtc atttgctatt ccagttactt ctgtcgaaga ggcaaaggat
      361 atattgaaag attataggaa acagtactac gatgctcgcc atatttgcta tgcttatgtt
      421 attggttatc caaattccgt tgtgcgtagt agcgatgatg gagaaccatc tggcacagca
      481 ggaaaaccca ttttagctca gatacaagcc aagcaattgt cggatgttat ggttgttgtg
      541 gtgcgttatt ttggcggtgt attattaggt acaggaggat tggtggtggc ttatcgtgaa
      601 gctaccgtag atgcattgaa taatactacc gtttatacaa aaattgtaga acaagcacta
      661 actattcaat gtgcctatcc acaactgaat gaagttatga aaattattaa agataataat
      721 ttgactatag tatcgcaaac attagaaata tcttgctcgt tgaaagtaca aattcgatta
      781 tccaactttg aaactatcac agagagattg aagcaggtgc atggattgca ggtaattgaa
      841 taaatatagt tgtgttgtta agttttacca aattaaaagg gcatttcaat ttccagaaat
      901 gcccttttac atactactta atttctataa ttttacttgg ttttggctta gcttcttccc
      961 ttttaggaat atcaacagct aaaataccat tttcgtaatt cgcacttatt ttttcagtat
     1021 cagcactaat gggtagagtg aatgaacgac aaaacgattg ataactaaat tctttacggg
     1081 taaaacgttc tccttctttg gtttcgtttt ctactttttt ttcactacat actgttagta
     1141 cgttgttgtt aacttcaatt ttgaaatcgt ctttggcaaa actaggtgca gcaatatgaa
     1201 tttcaaattt ttctgcactt tccttgatgt ttgctgaggg tacagtagtg ttcgtcgttg
     1261 aataattttc acttgtccaa tcgtaaaaat tattgtcgaa aaaacgatca aaaattgttg
     1321 gataattgtt tgagaatttt actagtgaca taacttttcc tcctataaat taatggttaa
     1381 acatttgttt tacactaact aaatatcaaa aaaaatgcca aacttataga gtatttagct
     1441 tggcattttt ttattagaaa agattttttt gttctgtcat tttggcagtt ttatcacttc
     1501 gtattttcag cttttggttt acaatacgat gaacaaatct ttgcatactg attgcgtaat
     1561 aagtagtctg ccgaatattt tcctgctcca atgagagcta ttataacata tatacccata
     1621 tatagcaatg ccatttcttt accactaaat gggtcgtttg catgaatgaa gaaagctgct
     1681 acacccattc ctataataag agggatagtg gatagacgtg taaataagcc cagcattaat
     1741 aggatggaac aaaccacttc ggcaaataca atcatgccga gcgataagct tgaacctact
     1801 cctaatgggt cgggaaatac agtacttagg aactcaaagt tttgtaattt tggtaaacca
     1861 tgtgttagca taaatccgcc aacaagtaat ctaagggcta atttgccaat ttgttgcgag
     1921 tactctgaac tagaggtgca acaagatgaa ttattttctg ttttcataaa attgtttttt
     1981 ttgttgataa ttaattgaac tgtatgggta ataaaaacgt aagcggaatg tgtaagcgtt
     2041 cacgccacgc attttctgaa tgatcttttc cttcaaattt tcgtgttatc caattggtag
     2101 aattgtatcc tttggcttgc ataattgcat caacttgttg ttggtacggt tcgtaatgaa
     2161 catctaatgt ttcggttcca aaatcgaagt aaaatttgtg tgtatctggt gagggtacat
     2221 tttcttttaa ataggcaaaa aattgagcag gaattgggtt tccttctgtt tgaaaactac
     2281 caatccaatg tgtagataaa caagctgcac caccaaaaac ttctgggtat tcgcatacag
     2341 catacatcga aataagtccg cccatgctag agcctgcaat aaatgtatgt tcaggttcgg
     2401 taagcgtagc atacgttttg tcaataaaag gttttagttc ctctacaata aaacgtaggt
     2461 aattatccga ttgcacacgt tcggcaaata atgaaacatt attttgtctt ctacccaata
     2521 aaattaatga gtcttgtgta gatacaggca agcgttcgaa tggtttttgt ggaaaataat
     2581 ccgaatgacg cgttcgtcca gaattccata ttcctactac aatggtactc agaatactgt
     2641 cgtttgctat aaggcgggta atggtttcgt ccacacccca ttcttgtttg ttccaggtag
     2701 tattggcatc aaaaagcatt tgtccatcgt gcatatagag cactttgtaa cgttggtttg
     2761 ggctgtaatt gtcaggtagc caaacatcta cattgcgagc agatatatag ttagaagtaa
     2821 aatctacatg acgtataatg gtaccactgc ttacacgggg taaggtattt tcttggctgt
     2881 acgttgtact aataaagaaa taaaaaagaa cgataaggca aagtaagtgt tttttcatgc
     2941 gtatatataa tttgttctga aaacaaatat acgtacaatt ttctcaaacg ctcacttctt
     3001 ctaccaattt cttaacataa ttttttgttt cataatccga atgtaacact tttttatcac
     3061 agagaatcac atcaatatca atgggaatga tgccttgcaa ggttttttca tccgaacgac
     3121 caagttgctt ttccatttct tttattttct gtattagctc tgttggctca agcctagttt
     3181 gtatgcaaac agccttattt acaaagggat agtcgtttgg catatcaatg gctttcgttt
     3241 ccatttcgtg cgaggtgcga taaataccaa attcttgttg cagcaattca atggctattg
     3301 acagatgttt gtgtgccaaa aaattgctgc caagcaataa gatgtaatgg tgagtgttca
     3361 tttctattta ttcatggcga tacgtgccat catttctatg ctacgtaatt tatctttgta
     3421 tgcattatga gcgttcattt tttctatatc taaagctgca tcagaattct cgttccacct
     3481 acgacacaaa tatactgaat tataaatgcg tccgatttgg tagtttctac taatctgaag
     3541 tcccaaagca taatcttctc catagctggt atttggcact ttgatttctc ttaatacagg
     3601 agtataaaac gctcgtggag ctcctaatcc attgatacga agtgcattgt ttcttccatt
     3661 ctcgggtgtc cattctttgt ggtcaattag tcctggagga atgggttgaa gggcaaaatc
     3721 cgtcatgcta tacgagccaa ttaccatggc acaattttgt tcgtaaaatg cgtttactat
     3781 ctgttgtagt gttgttgcgt tttcgtataa gtcatcgcta tctagttgaa cggcaaattt
     3841 tccacatttt tcgtggtgta caccttcgtt ccagcaacca cctataccca aatcgttgcg
     3901 ttgaggtatg atatgtacaa tagaagcgtt ctttttggca taagactgta ttaattctgt
     3961 tgttccatca gtagaatggt tgtcgataat gagcaaattg aatggaaaat tcgtttcttg
     4021 ttttaacaca gaaacaatgg catcttctat tgttttaacc ctatttttta ctggaataat
     4081 tacagaggct tcgtatgcga ataagtcttg gtgcaaatta actgctggaa aaatcggcgt
     4141 taagtaagct ccaatatttt taaggtgttg tgtacacact tgttccattt caatttgtac
     4201 cgctcggttt tttgggtcaa cgtaatcaaa ttgtttttcg ccacttttgc gggtatcttg
     4261 ttctatttcg gtatataaga attcgtttaa gtgtacaagt tgtgattgtt ctgatacggt
     4321 taatcggagt tgatacagcc cagcgtgggc aaaatctttt gagcagctag caacagatga
     4381 ttgcaatacc tttgtcgcaa aaaacaataa tgaaccaaaa ttaaaatcat cgcgaacact
     4441 cccaatttgg tagtcgttaa ccggaacttt agcagtgcct gtggttgttt gttggtaata
     4501 gtctgtatat accatacctg cctgcgtgtc gtttgctact tgtgccaatc gttctatagc
     4561 ataagctccc atgtataagg atgtatattt taggtagata agggcaaaat cattggtgca
     4621 attatcagca attaaccgca ttgttgccgt actggttagg gtgtcaattg ttagtgtttg
     4681 tgcatcgaat tttgtgctag ttgatgcttg ttttgttgct aacaaaaaaa tcttacctat
     4741 caaattactt ccttgtaacg aagcaattgt ttgagctact tgttcatcgt tttcgtaagg
     4801 gataaaagct gaaattttca tattcttgat gttatattga tgattaataa ttgacgaata
     4861 atcgcattag ttcgttttgt tgttcagtac ttgtgagtgt ttcgaaaggg aaaccacaaa
     4921 caatggtttt gtattttcct ttgtacccaa tggcagccgc tgtattgttt tcggtataac
     4981 gtagtaaaac ggaactattt ttatctgttg gttgtatggc atctacttgc tgcacagcat
     5041 attgataggg gtttggttgg gtaaaacaat tgtactcacg ctggcgtttt tttgcagaaa
     5101 acacgtcaac catcgttgca tttacggaag cctgtggcat gcgtagtttg tatttgagta
     5161 tgttttgtgc aaattgcatt tcgcttttgt ctttttttct tgttatgtca gatgcaatat
     5221 atgctccact tacgataatt tttccgtttt gtttggtgta attcgtaatt agtgtttgca
     5281 agtcggtagg atatacagta aaagtacgtg taaacgtttt actcgtttgt tgattaccaa
     5341 gtataatagc gagaacatcg tatccagtaa atgatggaga caataccgta atgccatcgc
     5401 gtgaagctga cgaaaatgaa tagttgtttg ccgctaaagc ccttccatga atgtagcaat
     5461 tatcaaaggt attcccagct attacttgtc catcgtagtt tgcttggcta gcaccaaatc
     5521 cagtataatc gttgtcgtaa aacggaactg atttattgta ttcaatttga ttgcctgtaa
     5581 aactcagatc gtacaaatag ggaacccctc tatccgtagc atgagtaaaa cctgcggtac
     5641 tatctgtttc aaaagaagaa ggtgcagcaa ccctatcaaa agcatttact acccaagcct
     5701 ttcctttttc aatttctgct ttgtacaccg ataaaatttc agaagcgaaa ctttctccgc
     5761 cattatttag agctgctatt ttataactat agatagtccc tttttcaatt ggtagcgtaa
     5821 tgctttcggt tttgctgaca attccattgt caaatcctcc atcgtttttg cgtgtatata
     5881 cgatataacc atcaggatta gctgttggtt caagcgggtc ggttgttttt cgccaagata
     5941 agtgtactga atcatcttgt aacacctctg ctacaaaatg agttatgggt aagggttgaa
     6001 ttgtataagg ttggttgtat tgagatgcta aaaacttaac tatccccttg tatatagcac
     6061 gagccgtaac aaaacgaaac tcagggcaat gagcataccg catatcgtta aaattttggt
     6121 gcgataataa ttcgataatg gtagtaggag tttttggtat gcgtgcttcg atataggact
     6181 tatcccaaat ttctctgcgt gtccagttta cgggaaaagt agcacgtata tcgtgtacaa
     6241 gttccgtttg aaccatatcc gtaaaatcgc gtgctgctat acgagagtag ttggctggat
     6301 acatgccttt atcatccgtt gtcatggcaa tgcccaaggt gccaacaatt gaatcgccac
     6361 tcatttttcc agcatcggta tgcaaggcga tgcacgcatc aatgggtatg ccaagtcctt
     6421 tgtagcttgg atacatagcc gaacctccca tcaagtaatt tacccagttg gctctaccaa
     6481 atatatcatc ttgataatca cttattacat ctttgcgttg gttgatgagg atactatcgg
     6541 gcatgccagc ccattgtaaa taatagcgag cggcttccgt aaaacgtgct ctaggagtag
     6601 aaataagggg tggttgagaa atagcgtctt cgtcgataaa gggaggaaat agcttttttt
     6661 ctacaatgct ttcggttgtt tgcctatcta tatttcccat gcctccacct acacgtacaa
     6721 catctgctgt tactacataa ccctctttgc cagagttggt taacaccaaa gaatacaatt
     6781 tatttgctat aaaataatag ttgcctaagt aaatccaagt gccaccgcct agttgttggt
     6841 tcacagaaaa tcgtgtagtt ccaccagcat gatgcaggct atagagagca gattcagtac
     6901 tgtttgaaac gcttttgtag gcaatagaca cgtaatacat gccgttttca ggaaccttaa
     6961 ttttccattc tatactatct gttgcttctt cgtaagtggg tgtttgtaaa aaagaaccca
     7021 tcgtaaaagg attatctctg ccttgtagta gtttcttgtt tttaaacccg ttttctttct
     7081 tttcccaact tgatgtctgt gtgattgagg cactggttgc gtcatcgtca ttatcaatta
     7141 ttacctcatt ggtttgtgta tcgcgttctc gtggaataaa tacattggct cctgcatttt
     7201 ctaacatagg caatacatag ggcagaacga tagctgttgt aagcatgtcc tcaaccgtgc
     7261 caaacaatct gcctcgttgc caatcccact tatcagtaat agcactgtaa taccagccat
     7321 gactattcca taatgcaata tgtctatgta gtaaaccttg tgtaggagag tacggtttag
     7381 atacattttg tattacgggt gtgatgtgcg gtggtgtaaa ttttctcgca ttatctattg
     7441 cgatagtctt tctgtaagca ttgggaacca attcatccaa ggtgcgatta ttgcatataa
     7501 tttcaagttg attctctcgg taggttgctg gcaattgtgc gtacactgag tcgtaaaaag
     7561 cctgaatgct ttcttctcta aaagggatat ttgctacatc attctctacc caaagggtta
     7621 aggtgttgtt tttgagtgtt tgttttgaaa cagaataaac tgtttttttt gctgtaatct
     7681 caggcgtgta acggatggtt tgaagcttat acttagcttg agccagcatc gaagttgaaa
     7741 atatccaaca actaacaaac aggattatat agtgccaacg aaaataggct tgcatcatct
     7801 aggtacgtgg tttattatat ggttcaaaaa tacacttttt tttttatttt tcagcagaga
     7861 caaacgtaaa atagtataat ttttacttac tttgtgcaag cgaaaagaga tacgtataga
     7921 aacataaaaa taagcgataa gatgaagtta ctagcagata gcggttccac aaaaacggct
     7981 tgggttgtgt taaatgaaaa tgggtcaaac gcagttgaat acataacaga tggcttaaat
     8041 cctttttttc agacagtaca gagtgtacaa caaaccattc aaaatcaact acttccccat
     8101 atagatgcta acagcatatc cgaaatttat ttttatggag caggttgtgc ctttcctgaa
     8161 aagaatgcta ttattgagga gggacttaga accgtgtttg cgagtgcaac tatccatgtg
     8221 gctagtgatt tgttaggtgc tgctcgtggc ttgtgcggta tgcaagatgg catagcttgt
     8281 attatgggca ctggttctaa ttcgtgtatg tatcaagata gtaagattgt tcacaatgtg
     8341 tcgcctctgg gatatatact aggagatgaa gggagtgggg cagtattggg taaattgtta
     8401 gtaggagatt acttaaaaaa tcaaatgcca actcatcttg cttctgcttt tttggctacc
     8461 taccagctta cgcctgctat tattttagat agggtgtata aacaagcgtt tccaaatcgg
     8521 tttttagcat cattcagtcc ttttttatct gcccataaac acgagccgta tgtacaggca
     8581 ttgctacacc gcagttttac tgattttttt gtaagaaata ttcaacaata tgattatcag
     8641 caccatactg cacattttac gggttctatt gcctattatt ttcaggatat cttgcagcag
     8701 gtagcacaag aacgaggtgt tgctgttggt gtaatagaac aaactccgat gccagggtta
     8761 gtgcggtttc atggggtgta ggtatcatag ttccaattgc taccattact gtcagaatga
     8821 gtaaatgttt cacctaagga tgcattaagg ttagggagtc gctccaattt cgactgttta
     8881 tatatctctt gatttgccga ttcatttaat agcgttattt gaagattgtt attcttttta
     8941 tacgccaatt gaatacaatc ttccaaagat aagttcacta tttcactagt agattgttga
     9001 gcaaacgtgg gtaagaccaa gaatgtaacg gtcggcggta tgtttttgtt gccgatttcg
     9061 aagcacaaca ctgtcaaacc
//
LOCUS       DAOSRP010000080         8961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7415_length_8961_cov_5.894678, whole genome shotgun sequence.
ACCESSION   DAOSRP010000080 DAOSRP010000000
VERSION     DAOSRP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7415_length_8961_cov_5.894678"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1018)
                     /locus_tag="PLJ82_06035"
     CDS             complement(113..1018)
                     /locus_tag="PLJ82_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOR41102.1"
                     /translation="MSEKYGYLLVNMLDMIKSYLTILVFIFVSMNVLAEQEHKMPPSN
                     TIGICLSGGGALGFSHIGVLQALEDNGIKPAYISGSSMGAIIGVLYAQGISPKEMMKI
                     IPKEKMNRFSRIWKLPSSILGFSSMITVHELLSKYVPHNRFDHLQIPLFVCVTNLYSG
                     EWEIKSTGDRLIDYVCASAAIPSVFEAQIIDSMHYVDGGVLNNLPVEPLLDKCDVIIG
                     VDVLEYPRKKELKKASDVVLYSILAANAETHRVRKPYCNYYISCNGASTYSIFDFHAF
                     KELYEIGYQRTVEYIQAHPEMLLHR"
     gene            1064..1753
                     /locus_tag="PLJ82_06040"
     CDS             1064..1753
                     /locus_tag="PLJ82_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOR41103.1"
                     /translation="MIGTLVNTAAVIVGGSLGMFIKHRLPEKYVRIYFQAVGLFTIVL
                     GISMSLKMAFPLMVVASLACGGILGEWLQLEEKTNRLGETLKKKLRFSNERFTEGLVT
                     AFLLFCMGSMSIIGAIEEGFGKTSDLLLTKSLMDGFSALMLASALGVGVVFSSIPLLL
                     FQGGITLMVWMFAKDIPTIYITEITAVGGILLIGLGFSILEIRKLNIINLLPSLVFIC
                     LFLYIRSYFAI"
     gene            1754..3886
                     /locus_tag="PLJ82_06045"
     CDS             1754..3886
                     /locus_tag="PLJ82_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOR41104.1"
                     /translation="MNATIISLIAKQLGISEKQVKNTADLLGSGNTIPFISRYRKETT
                     GNLDEVQIGNINELVQYFEELNKRKETILNTIEEQGNLTPELADKIATCWESNALEDM
                     YLPYKPKRKTKGQAAKENGLEPLAKILMSQSADDPYRIAQRFVTGNVQNNEAAVQGAL
                     DIIAEWINESAIARNRIRQIFAYDAVITANIIKGKEQEGAHYENYFKVKEPLKKCSSH
                     RLLAIRRGEAEGFLRVSINPSDEEVCLTKLTQIFVKNNTPSAQLVATACKDAYKRLLK
                     PSIETEFAHSSKELADKQAIAVFANNLKQLLLASPLGEKRVLALDPGYRTGCKVVCLD
                     AQGNLLHNETIYPHPPQKETAQAARKIQQLVETYAIQAIAIGNGTASRETETFMQHIR
                     YPLSVDIFVVSENGASVYSASKIARDEFPQYDVTVRGAISIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQNKLQTALDETVVHAVNLVGVNLNTASEHLLRYVSGLGPNLAKNIV
                     AYRSQNGEFTSREELKKVPRMGEKTFEQAAGFLRIAGAKNPLDNSAVHPESYYVVEKI
                     AKDLNCQLADLIGQKELAQRIQLERYIDQHIGLLSLNDILKELEKPGRDPRKKVSVIT
                     FDQRITTINDVQVGMLLDGIVTNITNFGVFVDIGIKENGLVHISQLANSYVSDPSTIV
                     SLHQAVQVKVIQVDTEKKRIQLSMKTGE"
     gene            4192..4538
                     /locus_tag="PLJ82_06050"
                     /pseudo
     CDS             4192..4538
                     /locus_tag="PLJ82_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4540..4863
                     /locus_tag="PLJ82_06055"
     CDS             4540..4863
                     /locus_tag="PLJ82_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41105.1"
                     /translation="MATNKFKTVSLDTMIDKHIGKRGTEKREKFENELRIDLLGQAIK
                     QARQACNMTQEELGELVGVQKAQISKIENSVKNARFETIMKVFEALGAKVSFKVELNN
                     KKLAY"
     gene            complement(4947..5777)
                     /locus_tag="PLJ82_06060"
     CDS             complement(4947..5777)
                     /locus_tag="PLJ82_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOR41106.1"
                     /translation="MQIGKYNTLRVVKHVDFGVYLDGGEFGEILLPIRYVPANCEVDD
                     RVDVFVYVDSEDRIIATTEKPFAEVGDFAALQVKSVTKFGAFLDWGLMKDLLVPFSEQ
                     KVYLEEGKLAVVYIYLDEKTNRIVGSAKLEKWVAKTPASFEENDQVEALIAKKTDLGY
                     KAIINNMYLGLIYQNEIFNPVAVGQKVPAFIKQVREDGKIDVRLQRSGAQHVMTEVER
                     ILAKLTDAGGFLPTTDKTAPEEIYATFGISKKSYKKVVGELYKRRLITIEEEGIRLVK
                     "
     gene            complement(5801..7378)
                     /locus_tag="PLJ82_06065"
     CDS             complement(5801..7378)
                     /locus_tag="PLJ82_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOR41107.1"
                     /translation="MTFQEEIKRRRTFAIISHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDWMEIEKQRGISVATSVMGFEYRDYKINILDTPGHQDFAEDTFRTLTA
                     VDSVIIVIDTAKGVETQTRKLMQVCRMRNTPVMVFVNKMDREGKEPFDLLDEIESELG
                     IAVRPLSWPINMGVGFKGVYNIYKKSLDLYTPNKQVISESIAFNDLSSKELEKHIGEN
                     NAAKLREDIELIEGVYEPFDVNTYLNGGLAPVFFGSALNNFGVKELLDCFVEIAPSPR
                     AVEATERVVKPEENNFSGFIFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRHNK
                     LMKFSSPTAFMAQKKETVDEAFAGDIIGLPDTGTFKIGDSLTAGEEIHFKGLPSFSPE
                     MFKYIENADPLKSKQLNKGIDQLMDEGVAQLFVNQYNGRKIIGTVGQLQFEVIQYRLL
                     HEYNAQCKWEPISLYKACWIESDNAEELEQFKKRKFQYMAKDKEGRDVFLADSGYVLQ
                     MAQSDFKNIRFHFTSEF"
     gene            complement(7671..8204)
                     /locus_tag="PLJ82_06070"
     CDS             complement(7671..8204)
                     /locus_tag="PLJ82_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41108.1"
                     /translation="MTKTIIIITTILFTSFIAISGKDSKNIQLRELQNFANNKDKVYH
                     LLDEKEGRLFIIYKNRISATISYNYENSEEYVFNELNITYVINGKDIASKLNSQKEVL
                     DFVKVEIDKNDFLIQTFFDEEFQNAYLGIDKPLGKSPGCLYYYTLESHIHGELFIKII
                     PMSKEQIIVSIHYALSL"
     gene            complement(8229..>8961)
                     /locus_tag="PLJ82_06075"
     CDS             complement(8229..>8961)
                     /locus_tag="PLJ82_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41109.1"
                     /translation="YGLIFIYGTTGELLMTIQIISDTPLQTTITWLNYLGIVFATEQQ
                     RQADKDLNISIKNKPEVEELWDGKVEKGKSNLKIIYSKDTDISVVTTEIENLLYRILK
                     NANIDREIKVTSAYRNPENQAKAMITLIKTKGLSKAKDLYNHKGDLVVGYYETIKNYE
                     QNEIIRLLVIKMNEVGFVSAHSDWKKRGAIDFGENSNGFNSIYSHEVNSIIESAKKEP
                     MVNPKQVLPPSNKEPALHIEIILNK"
BASE COUNT         2857 a         1760 c         1612 g         2732 t
ORIGIN      
        1 tgcttttatt ataggtacaa gttacgctat cgctaaactt gcaccaaaga ggggtaatga
       61 actttgaaca gctccaacta tcaactatca actatcaact atcaatcagt cttcatctat
      121 gcaacaacat ctctggatga gcctgtatat actctacagt acgctggtag ccaatctcat
      181 acaactcctt gaaggcatga aaatcaaaaa tcgaataggt agaagctcca ttgcacgata
      241 tatagtaatt gcagtagggt tttcgcaccc gatgggtttc ggcattggct gctaaaatag
      301 aatataaaac gacatcagag gcttttttta attccttttt tctgggatat tccaacacat
      361 ctacaccaat aatcacatcg cacttatcta acaatggctc aacgggcaaa ttatttaata
      421 cacccccatc tacatagtgc atggagtcga taatctgagc ctcaaacaca gagggtatag
      481 cagccgatgc acacacataa tcaatcaatc tatctcccgt tgatttaatc tcccattcgc
      541 ctgaatacag gttagttaca cacacaaaca agggtatttg caagtggtca aaacgattat
      601 ggggaacgta ttttgacaat agttcgtgta ccgtaatcat acttgaaaaa cccaaaatag
      661 aacttggtaa tttccaaata cgcgaaaagc gattcatttt ctctttcgga ataattttca
      721 tcatctcttt gggcgaaatg ccttgagcat acaatacccc aattatggca cccatactcg
      781 accccgaaat ataggcgggt tttataccat tgtcttcgag agcttgcaac acaccaatgt
      841 gcgaaaaacc taaggctcct cctccgctta aacaaatacc aatggtattc gatgggggca
      901 ttttatgttc ttgttctgct aatacgttca tagatacaaa aataaatact aaaattgtaa
      961 ggtagctttt tatcatatca agcatattta ccaacaaata tccgtatttt tcagacacaa
     1021 agcaattatt ttgttatttt tgtgtcgaat taattaaaat aagatgattg gaacactagt
     1081 aaatacagct gcggtaatag tgggaggcag tttgggtatg tttattaaac accgtttgcc
     1141 cgaaaaatac gtgcgtatct attttcaagc ggttgggttg tttaccatag tattgggcat
     1201 tagcatgtcg ctaaaaatgg catttccctt gatggtagta gccagtttgg cgtgtggcgg
     1261 catactcggc gaatggcttc agctcgaaga aaaaacaaac cgactgggcg aaactttaaa
     1321 aaagaaactg cgtttttcca acgagcgttt taccgaaggc ttagttacgg cttttttgct
     1381 tttttgtatg ggttccatgt caattattgg agccattgaa gaaggttttg gcaaaacatc
     1441 cgatttgtta ttaaccaaat ccttgatgga tggtttttct gccttgatgt tggcttcggc
     1501 attgggtgtt ggtgtggtat tttcatctat accgctactg ctatttcagg gaggtattac
     1561 gctgatggta tggatgtttg caaaagacat tcctacaatt tacatcaccg aaataacggc
     1621 tgttgggggt attttgttga ttggactagg tttttccatt ttagaaataa gaaaactcaa
     1681 catcatcaac ttactgccat cgcttgtctt tatttgtcta tttttatata tccgttcata
     1741 ttttgccatc taaatgaatg caaccatcat atcattgata gccaaacaac ttggcattag
     1801 cgaaaaacaa gtaaaaaaca cagccgactt gcttggttcg ggcaatacca ttccatttat
     1861 cagtcgctac cgcaaagaaa ctactggcaa cttagatgaa gtgcaaatag gcaatatcaa
     1921 tgaattagta cagtattttg aggaactaaa taaacgcaaa gaaaccatcc tcaacaccat
     1981 tgaagaacag gggaatctga ccccagaact tgctgataaa atagccactt gttgggaaag
     2041 caacgcacta gaagatatgt atttgcccta caagccaaaa cgtaaaacca aagggcaagc
     2101 cgccaaagaa aatggcttag aacccttggc aaaaatactg atgagtcaat cggcagacga
     2161 cccttatcgc attgctcagc gatttgttac aggcaatgta caaaacaatg aagcggctgt
     2221 gcaaggagcc ttggatatca ttgccgagtg gattaacgaa agtgccatcg cacgtaaccg
     2281 cattcgccaa atatttgctt acgatgctgt gattacagcc aatattatca aaggaaaaga
     2341 gcaagaaggg gctcattacg aaaattattt taaggtaaaa gagccgctaa aaaaatgtag
     2401 ctcgcaccgc ttattagcca ttcgtagagg cgaagctgaa ggatttttac gggtatccat
     2461 caatccgtct gacgaagagg tatgccttac aaaactgaca cagatttttg taaaaaacaa
     2521 cacgccatcg gctcaactcg tagctactgc ctgcaaagat gcgtataaac gtttgctaaa
     2581 accgtctatt gaaacagaat ttgcccatag cagtaaagag cttgccgaca aacaagccat
     2641 tgccgttttt gccaataacc tgaaacaatt gttattagca tctcctttgg gcgaaaaaag
     2701 ggtgcttgcc ctcgaccctg gctaccgaac agggtgtaaa gtggtatgtt tggatgctca
     2761 aggcaatttg ctacacaacg aaaccatcta tccgcatcca ccacaaaaag agaccgctca
     2821 ggctgcccgc aaaatacaac aattggtaga aacatacgcc atacaagcca ttgctattgg
     2881 caacggaact gcctccagag aaacggaaac gtttatgcaa cacattcgct atcccttgtc
     2941 agtagatatt tttgtcgtca gcgaaaatgg ggcttcggta tattcggctt ctaaaattgc
     3001 ccgcgatgag tttcctcaat acgatgtaac ggtgcgtgga gctatatcca ttggacgtag
     3061 gttgatggat cccttggctg aattggtaaa aatagaccca aaatccattg gcgtagggca
     3121 ataccaacac gatgtggatc agaataaatt gcaaacagca ttagacgaaa cggtagtaca
     3181 tgcggtaaat ttagtgggtg taaatttaaa tactgccagc gaacatttgt tgcgttatgt
     3241 gtcgggactg ggaccaaatt tggctaaaaa tattgtagcg tatcgttcgc aaaatggcga
     3301 atttaccagc agggaagagc taaaaaaagt gccacgcatg ggtgaaaaaa cctttgagca
     3361 agccgcagga tttttacgca ttgctggagc caaaaatccc ttggataact ctgctgtgca
     3421 ccccgaaagt tactatgtag tagaaaaaat agcaaaagac ctgaattgtc aattggctga
     3481 tttaattggt caaaaagagc tagcacaacg catacagctc gaacggtata tcgaccaaca
     3541 cataggttta ctttcgctca acgacatctt aaaagaactc gagaaaccgg gtagagatcc
     3601 ccgtaaaaaa gtcagtgtaa tcactttcga ccaacgcatt actactatca acgatgtgca
     3661 agtaggcatg ttacttgacg ggattgtaac caatatcacc aactttggtg tgtttgtaga
     3721 tatcggtatc aaggaaaatg gcttggtgca tatttcgcag ttagcaaaca gctatgtatc
     3781 tgacccaagc accattgtat ccttgcatca agctgtgcaa gtaaaagtaa tacaagtaga
     3841 tacggaaaag aaacggattc agctcagcat gaaaacgggc gaataaaggg aatgtaaaac
     3901 aatcatttca ccaaccgaat accctcttcc tctatggtta tcaaacgtct tttgtacaat
     3961 tcgcctattc cctcctcttg ccgtagtttg tattatcgca ctacacgctg agcgtatgcc
     4021 tattgcatac gtttagtgca taggaacaca accatgttaa agagcttacg tagaacgagg
     4081 attacaatct atgaagtttc aaacattgat ataaaacaac aaaaaatgaa aatttattac
     4141 aaaaaacttg tttcctaata aggaaacagc tatctttgac aaaaattatg aatgaaacca
     4201 aactttgaca tagaattatt gcctgaagcg gtagaatttt tagaaaacct tgacgaaaaa
     4261 tcaagagaaa aatttattac actatcaaaa aagcacaatt tgtaaacgac aacgaattat
     4321 tcaaaaagtt aaacgatttc atttgggaat ttagaacttt atataaagga aaagcatttc
     4381 gactgttttc gttttgggac aaatcaaacg ggagagaaac cttgattgtg gctactcacg
     4441 gaattttaaa gaaaacacag aaaacaccaa caaaagagat taaaaaagcc gaagaaataa
     4501 gaaaacaata tttagaatac aagtcaaaaa ataaataata tggcaacaaa taagtttaaa
     4561 accgtttcgc ttgataccat gatagacaaa cacattggaa agcgtggaac tgaaaagcga
     4621 gaaaaatttg agaatgagtt gagaattgac ctcttgggac aagccataaa acaagctcga
     4681 caagcatgta atatgacaca agaagaatta ggggagcttg tgggtgttca aaaagcacaa
     4741 atttcaaaaa ttgaaaacag tgttaaaaat gcaagatttg aaactattat gaaagtgttt
     4801 gaagccttgg gtgctaaggt aagttttaag gtagaactga ataataaaaa attagcatac
     4861 taaaatgcat tccatctgga gttcttgctt gccacacgat tcattttgtg cggtagcgta
     4921 gggcaaataa agggaatgta aaacaattat ttcaccaacc gaataccctc ttcctctatg
     4981 gttatcaaac gtcttttgta caattcgcct actacttttt tgtacgattt tttgctaatg
     5041 ccaaaggtgg cataaatttc ttctggagca gttttgtcgg tagtaggtaa aaagccgcca
     5101 gcatcagtaa gctttgccaa aatacgttct acctctgtca ttacatgctg tgctcctgaa
     5161 cgttgcaaac gaacatcaat cttgccatct tcccgtactt gcttaataaa agcaggcact
     5221 ttctgtccaa cagcaacggg attaaaaatt tcattttgat aaatcaaacc caaatacata
     5281 ttgttgatga tagctttgta gcctaaatcg gtttttttcg caatcaaggc ttctacttgg
     5341 tcgttttctt cgaatgaagc cggtgttttt gcaacccatt tttcgagttt tgccgaaccc
     5401 actatgcggt tggttttttc gtctaaataa atatacacca cagccaattt accctcttct
     5461 aaatacactt tttgttcgct aaatggcacc aacaagtctt tcatcagccc ccaatctaaa
     5521 aaagccccaa atttggttac gcttttcacc tgtaaagcgg caaaatcgcc aacttcggca
     5581 aaaggttttt cggtagtggc aataatgcgg tcttccgaat ccacatacac aaacacatcc
     5641 actcggtcgt ccacttcgca attggcagga acataccgta tcggcaacaa aatttcgcca
     5701 aactcgccgc catcgaggta aaccccaaaa tcaacatgtt tcactacacg taaggtattg
     5761 tattttccta tttgcatggt atttgtatgt gttatatttt ttaaaattcg gacgtaaaat
     5821 ggaaacgtat attcttaaaa tcgctttgtg ccatttgcaa tacataaccc gaatcggcta
     5881 aaaagacatc tctgccctct ttgtcttttg ccatgtattg gaacttgcgt tttttaaatt
     5941 gctctaattc ttcggcatta tcactttcta tccaacaagc tttgtacaaa ctgattggct
     6001 cccatttaca ctgagcattg tactcgtgca acaaacgata ctgaattact tcaaattgta
     6061 attgaccaac tgtaccaata atttttctgc cattatattg gtttacaaat aattgagcca
     6121 caccttcgtc cattaattgg tcaataccct tgtttaattg ctttgatttt aatgggtcgg
     6181 cattttcgat gtacttaaac atttcgggag agaaactggg taatcctttg aaatgaattt
     6241 cttcacctgc ggttaaactg tcgccaattt taaacgttcc tgtatcgggc aaaccgataa
     6301 tatcgcctgc aaaagcctca tcaacagttt ctttcttttg tgccatgaaa gcggtaggcg
     6361 atgaaaattt catcaactta ttatgacgaa tgtgtttgta gttgacatta cgctcaaact
     6421 tgcctgaaca tatttttaca aaagcaatac agctacgatg attggggtcc atattggcat
     6481 gaatcttaaa aataaaccca gagaaattat tctcttcagg ttttacaacc cgctcagtag
     6541 cctctacggc acgtggcgat ggggctattt caacaaaaca atccaacaat tcttttaccc
     6601 caaaattatt caaagcacta ccaaaaaata cgggtgccaa accaccatta agataggtat
     6661 ttacatcaaa aggctcgtac actccttcga taagctcgat atcttcacgc aatttagcgg
     6721 cattattttc tccaatgtgt ttttctaact ctttgctcga aaggtcgtta aaggcaattg
     6781 actcagaaat aacttgcttg ttgggagtat ataggtctaa actttttttg taaatattgt
     6841 acaccccttt aaaaccaacc cccatattga tgggccaact caaaggacga acagcaatac
     6901 ccaattcact ttcaatctcg tcgagcaaat cgaaaggttc ttttccttcc ctatccattt
     6961 tatttacaaa aaccattaca ggcgtattac gcatacgaca aacttgcatc agtttacgcg
     7021 tttgtgtttc tacccccttg gcggtatcaa tcacaataat tacgctatct accgcagtaa
     7081 gtgtacgaaa cgtatcctcg gcaaaatctt ggtgaccagg cgtatccaaa atattgattt
     7141 tataatctcg atattcgaac cccattaccg atgttgccac cgaaataccc cgttgttttt
     7201 caatttccat ccagtcagac gtggccgttt ttttgatttt gttcgatttt accgctcccg
     7261 caatatgaat agctccacca aaaagcaaga gtttttcggt taaagtagtc ttaccagcat
     7321 cgggatggct gatgatagca aaagtgcgtc ttcgttttat ttcttcttga aaagtcatgt
     7381 tatcgtgcta aaattgaggt gcaaagataa taaaaatccg cttacgaaat tgtaagaact
     7441 tgtaaagttt gaaagtttat aaagtttgaa agtagtagat agttgataat aaagaaaagc
     7501 cacacgagag acggggaacg ttgttcgttt agcgtttatc gttgttcgtg aggtagcggt
     7561 agtttggttt gaatagggaa agctggagct ttccttttaa tttcgacata gcgagacgct
     7621 acggcgaaca cttagattgt taaacttcac actacggcga acaaaggtgg ctataaagaa
     7681 agtgcataat ggatacttac aattatttgt tcttttgaca ttggaataat tttaataaat
     7741 agttcgccat gaatatgact ttctaaagta tagtagtaaa gacaaccagg agattttcct
     7801 aatggtttat caattcctaa atatgcattt tgaaattctt catcaaaaaa ggtttgaatt
     7861 aagaaatcgt tcttatctat ctcaactttt acaaaatcaa gcacttcttt ttggctattt
     7921 aatttgcttg ctatatcctt tccgttgatt acataggtga tatttaattc gttaaaaaca
     7981 tattcttcgc tgttttcata attgtaagaa atagttgcac taattctatt cttgtaaata
     8041 atgaatagtc tgccttcttt ctcgtctaac aaatgatata ctttgtcttt gttattggca
     8101 aagttttgta actctcgtag ctgaatattt ttactatcct ttccagaaat tgctatgaaa
     8161 gaagtaaata agatagtagt aataataata attgttttag tcataattgt aaattttaat
     8221 aagaaagttc atttgtttaa tattatttcg atatgtaaag cgggttcttt atttgatggt
     8281 ggaagaactt gttttggatt taccattggt tctttctttg cactttctat gattgaatta
     8341 acttcatgtg aataaatact attgaaccca ttagaatttt caccaaaatc tattgctccg
     8401 cgttttttcc aatcactatg tgccgaaacg aatcccactt cgttcatttt aataaccaat
     8461 aatctaatta tttcattttg ttcatagttt tttatagttt cataatagcc aacaactaaa
     8521 tcacctttat gattatacaa gtcttttgct ttactaagtc cttttgtttt tataagtgtt
     8581 atcattgctt ttgcttgatt ttcaggattt ctatatgcag atgtaacttt tatttctctg
     8641 tcaatattgg cattttttaa tattctataa agcaaatttt ctatttcagt tgttaccact
     8701 gaaatatcag tgtccttcga ataaataatc ttgagattac tctttccttt ttctaccttt
     8761 ccatcccaca actcttcaac ctcgggtttg ttttttatag aaatatttaa gtctttatct
     8821 gcttgccgct gctgttcggt agcaaaaaca atacccaaat aattcaacca agtaattgtg
     8881 gtttgtaagg gagtatcaga tattatttga attgtcatca acaactctcc tgttgttcca
     8941 tatataaata ttaaaccata c
//
LOCUS       DAOSRP010000081         8951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7423_length_8951_cov_4.421875, whole genome shotgun sequence.
ACCESSION   DAOSRP010000081 DAOSRP010000000
VERSION     DAOSRP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7423_length_8951_cov_4.421875"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="murB"
                     /locus_tag="PLJ82_06080"
     CDS             <1..790
                     /gene="murB"
                     /locus_tag="PLJ82_06080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOR41110.1"
                     /translation="SKESADHVWIRVGAGENWDDFVDYCVENQLGGAENLSLIPGEVG
                     ATPIQNIGAYGVEVKDIIQSVDCIEISTGKQKTFSNKECAFGYRDSIFKQGFRNKYVV
                     CYVTYKLAKHYTPNVSYGSLSTEILNYKEVNIQTVRQAVIAIRKRKLPEPKVLGNAGS
                     FFMNPIIAMEHFLQLQQLYPDIPSFHVSDKHIKVPAGWLIEQCGWKGKSIGKVAVHGS
                     QALVLINKGGANGLDIIYLSDKIRESVKEKFNIDIIPEVNIIFG"
     gene            complement(777..1967)
                     /locus_tag="PLJ82_06085"
     CDS             complement(777..1967)
                     /locus_tag="PLJ82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF037043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOR41111.1"
                     /translation="MLPQEIKHLIQRYKDKPLESVVFKKFPENVDVPLLLQQLEGRRF
                     IANKMPSWNTFIDELVFPSHLAFQQASSEQTARYKASLLQIESLVDLTGGMGIDSYFF
                     SQRVKKLVYIEQQSNLCSIASHNFNVLGAQNIDVINTQAEVYSNTMPMVDCVYIDPAR
                     RNITGAKVVLLEDCSPNVEAILPHILTKTRYVLLKLSPMFDIEMAVRSLPNIKEIHIV
                     AVDNDCKELLVVLDVLHSLPSCTYHCINIAENNQNQQHSYTNELVQDVHIHYTSQLST
                     YVYEPNVTILKAGCFSSIATAFCVEKLHPNSHLFTSNQLHIDFPGRIFEVIEVVEFNK
                     KAIKTLLTPIKKANVTVRNFPLRVDEFRKKTQLKEGGDTYIFATTVANNKKVCIICRK
                     INQK"
     gene            complement(1967..3433)
                     /locus_tag="PLJ82_06090"
     CDS             complement(1967..3433)
                     /locus_tag="PLJ82_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOR41112.1"
                     /translation="MKKILNIKIIIRTLGFLLLFQSFFMLLASFVSFLYNESDVQPIL
                     LSAGITLTVALIATLSVSKPTEPIGKREAYLVVALVWVVFSLFGLLPFLLSGSIPSFT
                     DAFFETMSGFTTTGASILTDVESLSHGLLFWRSIIQWLGGMGIIVLSLAILPLLGMGG
                     MQLFVAEVPGPTKDKLHAKVSFTAKALWGIYVVLTASQVLFLWLGGMDLFDGICHSFT
                     TMATGGFSTKNASIAYWNSPLIEYIIIVYMFLAGVNFSLYFSFLQGNFSKIFKNEELK
                     YYLSFVLVFTLLVVCSVVFYTPELVISVEQIIRHSLFQVVSLFTTTGFATYDYLLWSQ
                     FTWMLLIVTMLFGASAGSTSGGIKLVRIVLLLKNSYYEFKRLLHPNAIIPVRYNEKVV
                     MPQMMTNVLAFVALYITILVIGMIMLGAFGMNFDEAVGSAVTALSNVGPSLGSLGPSS
                     NFAAISLPAKWVMSFLMLIGRLELFTVLVILTPTFWSK"
     gene            complement(3442..4782)
                     /gene="trkA"
                     /locus_tag="PLJ82_06095"
     CDS             complement(3442..4782)
                     /gene="trkA"
                     /locus_tag="PLJ82_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOR41113.1"
                     /translation="MRIIIAGAGEVGTHLAKLLSKENHTIILLDENGEKVKALEANYD
                     ILGIEGLPTSLKDLKEAQVENADLFVAVTPYESTNITACLLATNLGAKKTLARIDNYE
                     YLLPKNLEFFQRLGVDSLIYPEMLAAKEIVSALKRGWVRQYVEFSDGALVLLATKLRE
                     NAPIVNKTIEEAFDKENSTVRIIMIKRLGMDIIAKGKDKILAGDIVYFITTKDNIEQV
                     RIQAGKEDFEINNIMILGGGSRIGVRAIQELPRNKNIKVIEQNKEKSIRVIENTDNVL
                     VINGDGRDLELLKNEDIESYDAFVALTGNAETNILACLTAKNFGVKRTVAEVENLDYV
                     PLAENLNIGSIINKKSLAASHIYKLILGGNVSNVRCLTLADVEIVEFVAGEKSAIVKT
                     PIKDIRLPENVNIGGIIRDGVGFVAMGTSHIQPGDNVLVFCAEDTIRKVEKLFK"
     gene            complement(4804..6705)
                     /gene="dxs"
                     /locus_tag="PLJ82_06100"
     CDS             complement(4804..6705)
                     /gene="dxs"
                     /locus_tag="PLJ82_06100"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR41114.1"
                     /translation="MMQSTYPLLNTINYPSDLRKLKECQLEQLCVELRQFLIDELSCN
                     PGHFGSSLGVVELTVALHYVFETPHDRIVWDVGHQAYGHKILTGRRNVFKTNRKFNGI
                     SGFPSPKESQYDSFVAGHASTSISAGLGMSIADAMLGEKRQVIAIIGDGSLTGGLAFE
                     GLNNASVSPNNLLIVLNDNNMSIDKNVGGLSQYLMEITTSRTYNRIRYKVYRAFEKIG
                     LMNESKRKSLLRFSNSIKNLLIGGNQNVFESLSIRYFGQVDGHDVLQLVKILNDIKDF
                     KGPKVLHIKTKKGKGFAPAENAATEWHAPGLFNKETGERIVNTTKGTKEPPLYQDVFG
                     ETLLELAKVNDKIVGVTPAMPTGCSMNILMKEMPDRAFDVGIAEGHAVTFSAGLAKEG
                     LLPFCNVYSSFMQRAYDQVIHDVALQNLNVVFCLDRAGLVGADGPTHHGNFDLAYFRV
                     VPNLTIASPINEEELRNMMYTAQLPNKGPFVIRYPRGNGVLVDWQTPMKELPIGKGVC
                     LKEGKDIAVLSIGNIGNTVAKAIELLGSDSKKVAHYDMRFLKPIDEEMLHDIASKFKK
                     VITVENGTIKGGLGSAVLEFFADNNYLLSVTRLGLPDEFIQHGSNDELFKMLRLDDQG
                     IAQSLRLLV"
     gene            6884..7849
                     /locus_tag="PLJ82_06105"
     CDS             6884..7849
                     /locus_tag="PLJ82_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR41115.1"
                     /translation="MKHFLFLIGFSILFGSCVTQQKYNELKESEERYYDEARLYEQEL
                     YELRNKHKDVSSELAKMKILKEKAEADTAKLAQELERLRFDCLNLQEQSKELVEKLRN
                     SKSEEEFKSLMAEVHSLQDELMRREDVLFKAERLLLEKQKELDLQNNKMNELNAMIAQ
                     KEQKLNEIKLLITNALHGLNGNGLSIEMKNGRIYVSLDEDLLFQSGRWEVDNRGVQAL
                     NRLANVLAQNKDIHIMVEGHTDDVPYKGTGNITDNWDLSVKRATSIVRILLNNRDIAP
                     TRITAAGRGEYMPVATGRTADARKKNRRTEIILSPNLDELMKIFE"
     gene            7852..8796
                     /locus_tag="PLJ82_06110"
     CDS             7852..8796
                     /locus_tag="PLJ82_06110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOR41116.1"
                     /translation="MEFRTLIQTSKSDYSISHTGSTMLLGSCFAQHIANKLRQHKFNV
                     ATPFGVLYNPLSIANSLYFLLNKTQLTDKDIFLQHDVWKSYAFHSAYNNTSAQGLLHT
                     LQEEINQSHTFLQQCNTLIITLGTAYVYYLNETNQVVANCHKTDAKAFTRKRVSLNEI
                     VHTYTHLLTTLKAFNPSLQVICTVSPIRHWKDGAHSNQLSKSILLLAIESLQEQFDFV
                     NYFPAYELVLDDLRDYRFYEQDMLHPNTTAIDYVWQHFSNTYFSEETKEINSKVYALT
                     KNEIHKPISPESERYKEFMEQHAKKVHALRTQYPFLKL"
BASE COUNT         3108 a         1653 c         1398 g         2792 t
ORIGIN      
        1 aagcaaggaa tcagctgacc acgtatggat acgtgtaggt gcaggagaaa attgggatga
       61 ttttgtagat tattgcgtag aaaatcaatt aggaggtgct gaaaatttat ctttaattcc
      121 aggcgaggtt ggtgctactc caattcaaaa tattggggct tatggtgttg aagtaaaaga
      181 tattattcaa tcagtagact gtatcgaaat tagcactggg aaacaaaaaa ccttttcaaa
      241 taaagagtgt gcttttggat acagggatag tatattcaaa cagggtttcc gtaataaata
      301 tgttgtttgc tatgttacgt acaaattagc taaacattat acccccaatg taagttatgg
      361 aagtttatct actgaaatct tgaattataa agaagtgaac atacaaacgg ttagacaagc
      421 cgtaattgcc atccgaaaac gaaaactccc tgaacctaag gtacttggta atgctggtag
      481 tttttttatg aatcccatca tagcaatgga acattttctt caattacaac agttgtatcc
      541 agatatccca agttttcatg tatctgataa gcatattaaa gttcctgcag gttggttaat
      601 tgaacaatgt ggatggaaag gaaaatcaat tggcaaagta gcagtacacg gtagccaagc
      661 attggtacta atcaacaaag gtggagcaaa tggattagac attatttatc tttcggataa
      721 aataagagaa tctgtaaaag agaaatttaa tattgacatt atacctgaag taaacattat
      781 ttttggttaa tttttcgaca gataatacat acttttttat tatttgctac cgttgtagca
      841 aatatatatg tatcaccacc ctcttttaat tgtgtttttt tacggaattc atccactcgt
      901 aaaggaaaat ttcgcaccgt tacgttagct tttttaatgg gtgttagtag tgttttgata
      961 gcctttttat taaattctac cacttctatt acctcaaata ttctacccgg aaaatcaata
     1021 tgcaattgat ttgaagtaaa taaatgacta ttgggatgaa gcttttctac acaaaatgca
     1081 gttgcaatac tactaaaaca acctgctttt aaaatggtaa catttggttc gtatacatag
     1141 gtcgaaagtt gagatgtata atgaatgtgt acgtcttgta caagctcatt tgtataacta
     1201 tgttgttgat tttggttgtt ttcagcaata ttgatacagt gatacgtaca actaggtaac
     1261 gaatgaagaa catcaagcac aacaagcaat tctttacaat cattatcaac tgctactatg
     1321 tgtatttcct ttatatttgg caaacttctc accgccattt ctatatcaaa cataggtgat
     1381 aatttgagta atacatacct tgtttttgta agtatgtgag gtaatatagc ctccacatta
     1441 gggctacaat cttctaacaa tacgacttta gctcccgtaa tattcctgcg tgcagggtcg
     1501 atatacacac aatcgaccat tggcatagtg ttactatata cttcagcttg tgtatttata
     1561 acatcaatat tctgtgcacc caatacgtta aaattatgag atgctatgct acataagtta
     1621 ctttgttgtt caatatacac taattttttc actcgttgcg aaaagaaata actatctatg
     1681 cccatcccac ctgttaaatc aacaagagat tctatttgta gcaaagaggc tttgtagcgt
     1741 gctgtttgct cagaagatgc ttgttgaaaa gccaaatgcg aaggaaatac gagctcatcg
     1801 atgaatgtat tccaagaagg cattttatta gcaataaatc tacgaccttc taattgttgc
     1861 agtaacaatg ggacatctac attctctgga aattttttaa aaacaacaga ttctagaggt
     1921 ttatctttgt aacgttgaat aagatgctta atttcttgtg gtagcattat ttactccaaa
     1981 atgtgggtgt aagaataacc aaaactgtaa ataattctaa acgaccaatt aacattaaaa
     2041 aagacataac ccatttggca gggagagaaa tagcggcaaa attagatgat ggtccgagac
     2101 tacctaaact gggtcctacg ttgcttaatg cagtaacagc cgaacctacc gcctcatcaa
     2161 aattcattcc aaaagctcca agcataatca ttccaattac taatatggta atatagagag
     2221 caacaaaagc caacacgttt gtcatcattt gaggcataac tactttttca ttataacgta
     2281 ctggaataat tgcatttggg tgcaacaaac gtttaaactc ataataacta tttttgagta
     2341 ataacacaat gcgaaccaac tttattccac cactagttga tccagctgat gccccaaata
     2401 acatcgtaac aatcaacaac atccatgtaa attgagacca aagtaaataa tcataggtag
     2461 caaaacctgt tgttgtgaac aaagaaacca cttgaaataa actatgtcga ataatctgtt
     2521 caacacttat tactaactca ggagtataaa atacgacgct acaaactact aatagagtaa
     2581 atacaagaac aaatgaaaga tagtacttta attcttcatt tttaaaaatc tttgaaaaat
     2641 ttccctgtaa aaaagaaaaa tagagcgaaa aattaactcc tgccaaaaac atatacacaa
     2701 tgattatata ctcaatcaaa ggagaattcc aataagcaat actggcattt ttagtagaaa
     2761 aacctcccgt tgccattgtc gtaaatgaat gacaaatacc atcaaataaa tccatccccc
     2821 caagccataa aaacaagact tgcgatgctg ttaaaactac ataaataccc cacaatgctt
     2881 tagctgtaaa actaacttta gcatgcaatt tatctttggt aggtcctggt acttctgcaa
     2941 caaaaagctg cattccaccc atgcccaata gaggcaaaat agccaatgaa agcactataa
     3001 tacccatacc tcccagccat tggataatac tccgccaaaa caataatcca tgagaaagac
     3061 tttctacatc ggttaagata gatgctcccg tagtggtaaa accagacatt gtttcaaaaa
     3121 aagcatccgt gaaagaagga atggaaccac tcaataaaaa aggaagcagc ccaaacaatg
     3181 aaaaaacaac ccaaactaat gctactacta aataagcttc tcgttttcca ataggttctg
     3241 ttggtttgga aacagataag gtagcaataa gggcaacggt caaagtaatg cctgcagaca
     3301 ataaaatggg ctgtacatct gattcgttat acaaaaaaga aacgaaagat gccaacaaca
     3361 taaaaaagct ttgaaacaat aacaaaaagc ctaatgttcg aataattatt ttgatattta
     3421 aaattttttt cattaattga gctacttaaa taacttctct acttttctaa ttgtatcttc
     3481 agcacaaaaa acgagcacat tatcaccagg ttgaatatga gaagttccca tggcaacaaa
     3541 gcctactcca tctctaatta tacctccgat atttacattc tcaggcaaac gaatatcttt
     3601 aattggggtt ttaactatag ccgatttttc tcctgctaca aactctacaa tttctacatc
     3661 agcaagggtt aagcaacgta cattagacac gttgccacca agaattagtt tatatatatg
     3721 actggcagct aatgattttt tatttataat tgaccctata tttagatttt ctgccaaagg
     3781 tacataatct aagttttcga cttcagcaac cgtacgtttt accccaaaat ttttggctgt
     3841 taaacatgct aatatatttg tttctgcatt acccgtgagg gcgacaaaag catcatatga
     3901 ctcaatgtct tcgtttttca acaattctaa atcacgtcca tctccgttaa taaccaatac
     3961 attatcggta ttttcaatga cacgtatact tttttcttta ttttgttcaa taacctttat
     4021 atttttattc cttggtaatt cttgtatcgc acgtacacca atacgacttc ctcctcccaa
     4081 aatcataata ttattaatct caaagtcttc ttttcctgcc tgaatacgta cttgttcaat
     4141 attgtctttt gttgtgataa aatatacgat atctccagcc aatattttat cttttccttt
     4201 ggcaataata tccattccta atcgcttaat cataatgata cgcacggtag aattctcttt
     4261 atcaaaagcc tcttcaatag ttttatttac aataggggca ttttctctta atttggtagc
     4321 taacaatact aaagctccat cagaaaattc gacatattga cgtacccacc ctcttttgag
     4381 tgcacttaca atttctttgg ctgccaacat ctcaggatag ataagagaat ctactcccaa
     4441 acgttgaaaa aactccaaat ttttaggtaa tagatattca tagttatcta tacgagcgag
     4501 ggtcttttta gctcctaaat tggttgccaa aagacaagcc gtaatattag tactttcgta
     4561 tggagtaaca gcaacaaata aatctgcatt ttctacctga gcttctttca aatcttttaa
     4621 tgaggttggc aaaccttcaa taccgagtat atcataattt gcctctaagg cttttacttt
     4681 ttcaccattt tcatcaagca aaataatcgt atgattttct tttgaaagta atttagcaag
     4741 atgagtacct acttctcctg caccagcaat aataattctc ataattgtat tgtatgaaat
     4801 actttatact aataaacgta aactttgtgc tattccttga tcatccaaac gtagcatttt
     4861 aaataattca tcattggaac catgttgaat aaattcatca ggtaaaccca agcgagtaac
     4921 tgacagtaaa taattattat cagcaaaaaa ttctaaaaca gctgacccta atcctccttt
     4981 aatagtacca ttttcaactg taattacttt tttaaatttg ctagcaatat catgtagcat
     5041 ttcttcatct attggcttaa ggaatcgcat atcatagtgc gccacctttt tgctatcact
     5101 tccaagcagt tcaattgctt ttgctacggt atttccaata tttccaatgc tcaatacagc
     5161 aatgtctttg ccttctttca aacaaacacc ttttccaata ggtaattctt tcatcggggt
     5221 ttgccaatca actaataccc cattgccacg tggataacga ataacaaatg gacctttatt
     5281 tggcaattgt gctgtataca tcatattgcg taattcttct tcgtttatgg gagatgcaat
     5341 agttaaatta ggaaccaccc gaaagtatgc taaatcaaaa tttccatgat gagtgggtcc
     5401 gtcagcccca accaatccag ccctatccaa acaaaatacc acattcaggt tttgcaatgc
     5461 cacatcgtgg ataacttggt cgtatgctcg ttgcataaaa gaggaataca cattacagaa
     5521 tggtagcaat ccttctttag ctaaaccagc agagaaggta acagcgtgcc cttcggcaat
     5581 acctacatca aaagctctat caggcatttc tttcatcaaa atattcatag agcaacctgt
     5641 aggcatagca ggtgtaactc ctacaatctt atcatttacc ttggctaatt cgagtaaagt
     5701 ttcaccaaaa acatcttgat ataaaggagg ttctttggtt cctttagtag tattaacaat
     5761 gcgttctcca gtttctttgt taaataatcc gggtgcatgc cattcggttg ctgcgttttc
     5821 agctggggca aaccctttcc ctttttttgt tttaatatgt aaaacttttg gtcctttaaa
     5881 atctttaata tcattcaaaa tttttactaa ctgcaacacg tcatgtccat ctacttgccc
     5941 aaaatagcgt atactcaaac tttcaaaaac attttgattt cctccgataa gtaaattttt
     6001 gatactattc gaaaatcgaa gtaacgactt tctttttgac tcgttcatca agccaatttt
     6061 ttcaaaagct cgatatactt tgtaacgaat acgattgtat gtacgagaag tagtaatctc
     6121 cattaaatat tggcttaatc cacctacatt tttatctata gacatgttat tgtcattaag
     6181 cactatcaat aaattattgg gagaaacaga tgcattattc agtccttcaa aagccaatcc
     6241 tccagtcaaa gagccatctc caattatagc tattacctgc cttttttcgc caagcatagc
     6301 atcggcaata gacatgccta accctgctga aatagaggta gaagcatgcc cagcaacaaa
     6361 cgaatcgtat tgactctctt tgggagatgg aaaaccacta atgccgttga attttctatt
     6421 tgttttaaaa acatttcgtc taccagttaa tattttgtgt ccatatgctt gatgacctac
     6481 gtcccatact atccgatcgt gcggagtttc aaacacatag tgcaaagcaa cggttaattc
     6541 gacaacgcct aaacttgagc caaaatgacc tggattacac gataactcat caattaaaaa
     6601 ctgtcttaat tcaacacaaa gttgttccaa ttggcattct ttcaatttac gcaaatcaga
     6661 aggataatta atggtattta ataatggata tgttgattgc atcatcttta cttaaatttt
     6721 aaatgcaaaa gtacaaatta tattattgtt ttaatatttt taaaccttaa attatcagtt
     6781 agaatgatgt taaatgtgta catttgcgaa aatttacgat attgtgcatg cattttgtgc
     6841 atacttattt gttaagttat ttataatcaa aaacttttac tgtatgaaac atttcttatt
     6901 ccttattggt ttctccattt tatttggctc atgtgttaca caacaaaaat acaatgaatt
     6961 gaaagaaagt gaagaacgct attatgatga agctcgctta tatgaacaag aactttatga
     7021 attgcgtaat aaacacaaag atgtaagcag tgaacttgcc aaaatgaaga tattgaaaga
     7081 aaaagcggaa gcggataccg caaaacttgc gcaagagtta gaacgcttac gctttgattg
     7141 cttaaattta caagaacaaa gcaaagaatt agtagaaaaa ctccgtaact ctaaaagtga
     7201 agaagaattt aaatcattaa tggctgaagt acatagccta caagatgaat taatgcgtag
     7261 agaagatgta ctattcaaag cagaaagatt actattagag aaacaaaaag aattggattt
     7321 gcagaataac aaaatgaatg agttaaatgc aatgattgca caaaaagaac aaaaattaaa
     7381 tgaaattaaa cttctcatta ccaatgcttt gcatggatta aatggcaatg gattaagtat
     7441 agagatgaaa aatggtagaa tctatgtttc tcttgacgaa gatctattat ttcaatcagg
     7501 cagatgggag gtagataaca gaggagtgca agctcttaat cgtttagcaa acgtattggc
     7561 tcaaaacaaa gatattcata tcatggtaga gggtcataca gacgatgtac cttacaaagg
     7621 aacaggtaac attactgaca attgggattt aagcgtaaaa agagccacat ctatagttcg
     7681 tatattgctt aataacagag atatagcacc aactagaata acggctgcag gtagagggga
     7741 atatatgcca gtagctactg gacgaacagc tgatgctcgc aaaaaaaaca gacgcacaga
     7801 aataatactt tcgccaaatt tggacgaatt aatgaaaata tttgaataat agtggaattt
     7861 agaacgctta tacaaacatc caaatcggac tactctattt cgcatacagg gtctacgatg
     7921 ttgcttggtt cttgttttgc acaacatatt gccaataaat tacgacagca caaatttaat
     7981 gtagcaactc catttggcgt tttgtataac cctctttcga tagcaaatag cttgtacttt
     8041 ttgttaaaca agactcaatt gacagacaaa gacattttct tgcaacacga tgtttggaaa
     8101 agctatgcat ttcatagtgc atataacaac acgtcagcac aaggtttgct acacacttta
     8161 caagaagaaa taaatcaatc acatacattt ctacaacaat gtaatacctt gattataaca
     8221 cttggaactg cgtatgtata ttacctaaac gaaactaatc aggtagtagc aaattgccac
     8281 aaaacagacg ctaaagcatt tacaagaaag agagtttccc tcaatgagat tgtacacaca
     8341 tacactcatt tattaactac attaaaagca ttcaatccat cattacaggt tatctgtaca
     8401 gttagtccta tccgtcattg gaaagatggg gctcatagca accaactaag taaaagcatt
     8461 ttattactgg caatagaaag tttacaagag caatttgatt ttgtaaacta ttttccagca
     8521 tacgaacttg tattagatga cttacgcgat tatcgttttt acgaacaaga tatgctacat
     8581 ccaaatacaa ctgcgattga ctatgtatgg cagcatttta gcaacaccta tttttcggaa
     8641 gaaacaaaag aaattaatag caaagtatat gccctaacaa aaaacgagat acataaaccg
     8701 atatcaccag aaagtgaacg atacaaagaa tttatggaac aacatgcaaa aaaggtacat
     8761 gctttgcgta ctcaatatcc atttcttaaa ctataataca tgaaaacaaa cgaaaaagaa
     8821 aatccattta ttagcttact agtgggtatc gttattccag ctatcatttt atcaaaattt
     8881 agttcccccg aacaactggg cgttttattg ggttttattt tggctttatc cttcccttta
     8941 gtattagcat t
//
LOCUS       DAOSRP010000082         8853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7536_length_8853_cov_3.734712, whole genome shotgun sequence.
ACCESSION   DAOSRP010000082 DAOSRP010000000
VERSION     DAOSRP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_8853_cov_3.734712"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLJ82_06115"
     CDS             <1..326
                     /locus_tag="PLJ82_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41117.1"
                     /translation="LLIFTVLHVLQFKVKRTIEYVELDGVLKPDIYQFISHFFSSNWQ
                     YSLLYMFSGILLGVHLQNGFVSMIKKINIDKKYQKLISITIKITSYSIMIGFLLIPLY
                     FLFFS"
     gene            complement(330..1856)
                     /locus_tag="PLJ82_06120"
     CDS             complement(330..1856)
                     /locus_tag="PLJ82_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR41118.1"
                     /translation="MKIYRFVLFILFFLSIKAYTQTSEDYFNSGFAKSQQLDFEAAII
                     DFTKAISLDATFAEAYNHRGIANRYLKKYQVAITDYTKAIEIHPHYAEAYNNRGVVKH
                     QIQLYQSALEDYNRAIQLNSMYAEVYNNRGVTKHVLRDFQSALADFTKAIDIDPKYAE
                     AYNNRGVTRFQLEQYTEAIADYNTALAIKLSYAQVYFNRGLANRKSNELNEAIADYTK
                     ALEFTPQLAEAYNNRGVAKVKLEDYRGAIADYNSAIDVNPSYTKAYYNRGIVKMMLLD
                     YQGAIIDYNRALELNSNDAETYNNRGICKESVNDYQGALIDYNKAIELNPSFSEAYNN
                     RGVLYRKMNNYKAAMADYDKLLVLTPKNAEAYYHRGIVKYYISDLSGSITDFTQAITF
                     NSQLFEAYMYRGIVKNMLNDFSGAILDYNEAIKLNPESAEAYYNRGVSKGRLQEHQSS
                     IEDFTQAMNYKMNYAEAIFNRGVSYLALAQKDAACADFSKASQLGFYKAFEVIAVFCK
                     "
     gene            complement(1974..2330)
                     /locus_tag="PLJ82_06125"
     CDS             complement(1974..2330)
                     /locus_tag="PLJ82_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOR41119.1"
                     /translation="MKNKVITTILSLLVFLPMLVAQQPKAEKISATFMVNIHCHSCKE
                     KITTAMQEEKGLKKIKINMKDKTVAITFMSDKNSVSNLLSVFKKLGYVATIQEVSCIG
                     SKEGCLNAIHPESTMR"
     gene            complement(2341..4509)
                     /locus_tag="PLJ82_06130"
     CDS             complement(2341..4509)
                     /locus_tag="PLJ82_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41120.1"
                     /translation="MKTFYIFLIGLLVSASMHSQVIGKVLGENKEPLIGANIYWENTN
                     YGVLSSIDGNFEIEKMNTSDRLIISYLGYKSDTIQITYSSPYIVQLAQVTELDEVEVS
                     GRKSGLVTFRATPIQTQEISYSELCRAACCNLSESFETNPSVDVSYTDAATGAKQIRL
                     LGLSGIYVQMINENIPSFRGLGSTYGLSYVPGTWMDGILISKGTSSVSNGYESITGQI
                     NVEYKKPQLADPFSVNLFGSSDGRVELNADASTKLTKNLSTMLFVHADKDFQHHDKNN
                     DGFADYPQVEQYSFFNRWQYANKGYTAQFGAKGLYENRRGGEMIHHGYKINIDTWRAD
                     AFMKHGFELNKEKESSIGVIANGVFHRQESIYGIQTYDASQTSLYLNAIYQSHLTDEE
                     THLIKTGLSMEADLYEEKWNTQITETDYITPGIFGEYTLKLADKWIVLAGIRADYTKQ
                     YALFVTPRLHIKYMPINAVTLRFSIGKGYRSPNVMIENNNLLASNREFVISTPLQQEA
                     AWNTGVNAVFDIPIGYRTVMVSTEYYYTLFDNQLVIDFDSFANKILFYNALGTSYAHN
                     AQIEVSTEIADGLTILGAYRYNDTKSVTGGVLQLKPLMSMHRGLITASYKTSLGRWQY
                     DITSQLNGGGRMPTPDAINPLWNKTFSAYQQYNAQVTRYFKNASVYVGIENITNFTQS
                     NPIISASNPQSDTFDATMIWGPLHGRKFYLGLRYNLPKQN"
     gene            complement(4660..5007)
                     /locus_tag="PLJ82_06135"
     CDS             complement(4660..5007)
                     /locus_tag="PLJ82_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41121.1"
                     /translation="MGQSCMDVHHSVKGDLAICCTSSCDDSEAQDIPHKHDDHHQDCT
                     SQRLSIPLDSYIAKVQITQPLSTIPSFLLSAVLAIPSDIQQINGKKEYLSINLYKYYL
                     YEDYLSKIAVFII"
     gene            5232..5852
                     /gene="rsmG"
                     /locus_tag="PLJ82_06140"
     CDS             5232..5852
                     /gene="rsmG"
                     /locus_tag="PLJ82_06140"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOR41122.1"
                     /translation="MNVIIKYFPNLSIQQVAQFEALFPLYMDWNSKINVISRKDIENL
                     YVHHVLHSLAIAKIIPFVDQTSVIDIGTGGGFPGIPLAILFPNVQFHLVDSIGKKIKV
                     AQEIAQEIGLENTTFLHDRIENEKKTVDFVVSRAVMPLGDLVRFCKKNIKKEQKNSLP
                     NGLICLKGGELQSEIQAFKKSATIYELTDFFNEDYFKTKKAVYIPL"
     gene            5861..6487
                     /locus_tag="PLJ82_06145"
     CDS             5861..6487
                     /locus_tag="PLJ82_06145"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOR41123.1"
                     /translation="MHQLKYAARRKELVEILAMKGNFSTHVLQAISKVPRHLFMDKTL
                     IEFAYEDKAFPIGSNQTISQPYTVAYQSELLNIQKGDKVLEIGTGSGYQSAVLVELGA
                     QVFSIERQKVLFHHTYSLLPEIGYRIRMFHGDGYVGLPKYAPFDKIIVTASAEEIPQE
                     LIQQLKIGGIMVIPVGNTMFKVTKLTDTTTEKIEVDNCAFVPMLRGIN"
     gene            6515..7147
                     /locus_tag="PLJ82_06150"
     CDS             6515..7147
                     /locus_tag="PLJ82_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR41124.1"
                     /translation="MNVKTFIFNPFQENTYVLSDSNNKCIIIDAGCLNLEEKNDLYSY
                     IKENNLLVERILNTHLHIDHCFGNQFVSETFNKKIEAHPDDEALLNGMNKYAQTFGIE
                     YTENHPTISYIFEGDTITVGEISLKAIHVPGHSKGSLVFYAEKEKYLFAGDVLFSGSI
                     GRTDLPGGNFHDLIDNIKQKLLILPDETVVFSGHGAQTTILHEKQYNPFL"
     gene            7203..8702
                     /locus_tag="PLJ82_06155"
     CDS             7203..8702
                     /locus_tag="PLJ82_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR41125.1"
                     /translation="MKIFNSTAIRQIDAYTIEKNHIKSIELMEVAARSVFEYIIQHYD
                     IEKKIIIFAGQGNNGGDALALARLLHIAGFSIKVITVFLSDNLSDDCETNYKALLNKN
                     IPIYHYSRYLELNIKEDYLIIDGIFGTGLNKPITGKLADMIVYINSRNCEIIAIDIPS
                     GLPSDGVTETNPIAIKATITISFQFPKISFFFAEYESFIGKWILTNIGLDKEYIANKE
                     TNFYTITKERIQKILKKRSVFSHKGTFGHCLLMNGSYGKMGAGILSAKACLRSGVGLL
                     TTHIPVCGNTVMQIAVPEAMTYTDMQELTLTSVHDNINDFDAVGCGCGIGTAIHTQKM
                     IHNLLLSTNTPCVLDADALNCIALNNWKHLLPKNTIITPHPKEFDRLVGESKHNGERL
                     QKAILLSKQLTIVIILKGAYTRVVLPNGKVYFNTTGNPGMATAGSGDVLTGIIAGLRA
                     QKYDAIEASLLGVYLHGLAGDYAKEKYSEKSLVATDIIENISEAYKYIL"
BASE COUNT         3024 a         1517 c         1354 g         2958 t
ORIGIN      
        1 ttttattgat atttacggta ttgcatgtat tgcaatttaa agtaaaacgg acaatagaat
       61 acgtagagtt agatggggta ttaaagcctg atatctacca gtttatcagt catttctttt
      121 cgagtaattg gcaatactcc ttgctatata tgttttctgg gatattactt ggtgtgcatt
      181 tacaaaacgg atttgtatct atgataaaaa aaatcaacat tgataaaaaa tatcaaaaat
      241 taatctctat tacaattaaa ataacgagct attccattat gataggattc ttgctaatcc
      301 cattgtattt tctttttttc tcttgatttt tatttgcaga aaacggcaat gacctcaaaa
      361 gctttataaa aaccaagttg agatgctttg gaaaaatcgg cacatgctgc atctttttga
      421 gctaacgcta aataagatac tcctctatta aaaattgctt ctgcataatt cattttataa
      481 ttcatagcct gtgtaaaatc ttcaatagag gattgatgtt cttgcaatct tcctttagat
      541 acacccctat tgtaatacgc ttcagcagat tctgggttta atttgattgc ttcattgtaa
      601 tctaaaatag ccccactaaa atcattaagc atatttttta ctatacctct atacatatat
      661 gcttcaaaga gttgggagtt aaatgttatt gcttgtgtaa aatcagtaat agatccagat
      721 aaatcagaaa tatagtattt cacaatacct ctatgataat acgcttctgc atttttcgga
      781 gttaaaacaa gtaatttgtc ataatcagcc attgccgctt tataattatt cattttacga
      841 tataacacac ccctattgtt atacgcttca gaaaatgatg gattaagctc aatagcttta
      901 ttataatcaa ttaaagcccc ttgataatca tttacacttt ctttgcatat tcctctgtta
      961 ttataggttt cggcatcatt tgaatttaat tctaatgctc tattgtagtc aataattgcc
     1021 ccttgataat ccaataacat catcttcaca atacctctgt tataatacgc tttggtataa
     1081 cttggattaa catcaattgc gctattgtaa tcggcaatag cccctctata atcttctaat
     1141 tttacttttg ctacccctct attattgtaa gcttcagcta gctgaggagt aaactccaag
     1201 gctttggtat aatcagcaat agcctcattc aattcattag attttctatt tgctaaccct
     1261 ctattaaaat atacttgagc atacgataat ttaatagcta gggctgtatt ataatcagct
     1321 atagcttcag tatattgttc taactgaaat ctagttaccc ccctgttatt ataagcttcg
     1381 gcatatttag gatcaatatc aatagctttg gtaaaatcag ccaatgccga ctgaaaatca
     1441 cgtaacacgt gttttgtaac gcctctgttg ttatatactt cagcatacat actgttaagt
     1501 tgaatagctc tattataatc ctccaatgca ctctggtata attgtatctg atgtttaacc
     1561 actcctctat tattgtaagc ttcggcataa tggggatgta tttcaattgc ttttgtgtaa
     1621 tcagttattg caacttgata tttttttaaa tatctattgg caatgcctcg atgattatat
     1681 gcttcagcaa atgtggcatc taaagaaatt gcttttgtaa aatcaattat agccgcttca
     1741 aagtccaatt gttgagattt ggcaaaacca ctattgaaat aatcttccga cgtttgagta
     1801 taagctttta tagatagaaa aaaaagtata aataaaacga atctatatat tttcataatg
     1861 caaattttac aggttactga taatatcact ttaaagtgct attattaatt aaacgcaaat
     1921 ggaataaaaa aagtatcaca aataatagaa tgtgatactt tttttattac taattacctc
     1981 atggttgatt caggatgtat tgcgtttaaa caaccctcct tactccctat acaactaacc
     2041 tcttgaatag tagcaacata acctaatttt ttaaataccg acaataaatt acttactgaa
     2101 tttttatcag acataaaagt aattgcaacc gttttatcct tcatattaat tttaattttt
     2161 tttaatcctt tttcttcttg catagctgtc gtaatttttt ctttgcaaga atggcaatga
     2221 atatttacca taaacgttgc tgaaattttc tctgcttttg gttgttgtgc tactagcatt
     2281 ggtaaaaaca ctaacagaga taaaattgta gttataacct tgtttttcat atttatatat
     2341 ttaattttgt tttggtaaat tgtagcgtaa acccagataa aattttcttc cgtgcaaagg
     2401 tccccaaatc attgtagcat caaatgtatc tgattgtgga ttagaagcac taataattgg
     2461 atttgattgt gtaaagttag taatattttc aatacctaca tacacagatg catttttaaa
     2521 gtaacgagta acttgagcat tgtattgttg gtaggcacta aacgttttat tccataatgg
     2581 atttattgca tcaggcgttg gcatacgtcc gccaccattt aattgcgagg taatatcata
     2641 ctgccatctg cctaaggaag ttttgtaaga tgctgttatt aaacctctat gcatgctcat
     2701 tagtggtttt agttgtaata cacctcctgt tacagatttc gtatcattat atcgataagc
     2761 tccaagaatt gttaacccat ccgctatttc agtgctaact tctatttgtg cattgtgtgc
     2821 ataagacgtg cctaatgcat tgtaaaaaag gattttatta gcaaatgaat caaaatcaat
     2881 aacaagttga ttgtcaaata atgtataata atactctgta gaaaccatta ctgttcggta
     2941 gccaattgga atgtcaaata ctgcatttac acctgtattc caagcagctt cttgttgtag
     3001 tggagtagaa ataacaaact ctctgttact tgcaagtaag ttattattct ctatcattac
     3061 atttggcgat cgataccctt tacctataga aaaacgcaag gtaacagcat ttattggcat
     3121 gtatttaata tgcaaacgtg gggttacaaa tagagcatat tgtttagtat aatcagctcg
     3181 tatacctgcc aacacaatcc atttatctgc cagttttaat gtatactcac caaaaatacc
     3241 aggagtaatg tagtccgttt ctgttatctg ggtattccac ttctcttcat ataaatccgc
     3301 ttccatactt agccctgttt taattaaatg cgtttcttca tcagttaaat gactctgata
     3361 aattgcattt agatacaatg acgtttgtga tgcatcgtat gtttgaattc catatatgct
     3421 ttcttggcga tgaaatacac catttgctat cacaccaata ctcgactctt tttctttatt
     3481 caattcaaag ccatgtttca taaaagcatc tgctctccaa gtatctatat tgattttata
     3541 gccatgatgt atcatctctc cgcctcttct attttcgtat aagcctttag ctccaaactg
     3601 tgctgtgtag cccttgtttg catattgcca tctgttaaaa aatgaatatt gctctacttg
     3661 tggataatca gcgaatccat cattattttt atcatgatgt tgaaaatctt tatctgcgtg
     3721 cacaaatagc atcgttgaca aattctttgt cagcttagtg cttgcatcag cgttaagttc
     3781 aaccctacca tcgctactac caaacagatt cactgaaaat gggtctgcta actgaggttt
     3841 tttgtactca acattaatct gccctgtaat cgattcatat ccattagata cagaggatgt
     3901 tcccttagaa attaaaatac catccatcca cgtaccaggc acatacgata aaccataggt
     3961 actacctaat cctctaaaac ttggtatatt ttcatttatc atttgtacat aaatacctga
     4021 taaacctaac aacctaattt gctttgcacc tgtagctgca tctgtgtaag agacgtctac
     4081 cgaaggattt gtttcaaaac tctcagacaa attacaacaa gcagcacgac ataattctga
     4141 ataagatatc tcctgcgttt gaataggtgt agccctaaaa gttactagac ctgattttct
     4201 gcccgaaacc tctacttcat ctaattctgt aacttgtgcc aattgcacaa tatagggaga
     4261 agaatatgta atctgtattg tgtctgattt atatcctaaa taacttataa tcaaacgatc
     4321 cgatgtattc attttttcta tctcaaaatt tccatcaata gaagaaagaa caccataatt
     4381 tgtattttcc caataaatat tagcaccaat aaggggttct ttgttttctc ccaacacttt
     4441 gccaattact tgagaatgca tggatgcaga taccagcaat ccaattaaaa agatataaaa
     4501 tgttttcatt attaaaaaaa atgaagtgaa taaactaaaa aaaaaacgta aatgtgtaac
     4561 aaacacaccc ttttttctta cttttatctt gagtacaacc cattatatcg taaatggaca
     4621 caatgatgat acatagataa aactagagtt tagttcaaat caaataataa aaacagctat
     4681 tttggataaa taatcttcgt acaaatagta cttatacaaa ttaattgaaa ggtattcttt
     4741 tttaccatta atttgttgaa tatcagaagg tattgcaaga acagcagaaa gtaaaaaaga
     4801 cggaatagta gataaaggct gtgttatttg caccttagct atgtaactat ccaaaggtat
     4861 agataagcgt tgagatgtac aatcttgatg atgatcatca tgtttgtgag ggatatcttg
     4921 tgcttcgcta tcatcacaag aactagtaca acagatagca agatcaccct ttactgaatg
     4981 gtgtacatcc atgcatgact gacccattaa aataggaaaa tactccctac aagcatcaca
     5041 tccatagtga acaacattta ctcctaaact agcaaaaaag agtaatacgg caaaaaacta
     5101 taaaataaat cgtttcattg acaagaaagt agtgttaatt acttttttac aaagatacac
     5161 acaaaaaatt tttacagata tagaaaaaac gtatctttga cataaaatta tgaattttta
     5221 attttttaag catgaatgtg attattaaat acttcccaaa tttatcaata caacaagttg
     5281 ctcaatttga agcacttttt ccattatata tggattggaa tagcaaaatt aacgtcattt
     5341 cacgtaaaga tattgagaat ttatacgttc atcacgtatt acattcatta gcgatagcaa
     5401 agattatacc ttttgttgat caaacatctg ttattgacat tggcactggt ggcggttttc
     5461 ctggtattcc gttagctata ttgtttccta acgtacagtt tcatttagtt gattcaatcg
     5521 gtaaaaaaat taaagtagct caagaaatag ctcaagaaat tggacttgaa aatacaacct
     5581 ttctccacga tagaatcgaa aatgaaaaaa aaacagttga ttttgtggtt agtagagctg
     5641 ttatgcctct gggagattta gttcgttttt gtaaaaaaaa tattaaaaaa gaacaaaaaa
     5701 attccctccc taatggatta atatgtttaa aaggagggga acttcaatca gaaatacaag
     5761 cttttaaaaa aagtgcaaca atatatgagt taactgattt cttcaatgaa gattatttta
     5821 aaacaaaaaa agctgtttat attcctttgt aaaagttatt atgcatcaac taaaatacgc
     5881 cgcaagaaga aaagagttag tagaaatact agcaatgaaa ggaaatttct caacacatgt
     5941 attgcaagca attagcaaag ttcctcgaca tttatttatg gataaaacgc taattgagtt
     6001 tgcatacgaa gacaaagcat ttcctatagg atcaaaccaa actatttctc aaccttacac
     6061 tgttgcctac caatcggaat tactgaacat acaaaaagga gataaagtgt tagaaatagg
     6121 cacaggatct ggatatcaat cagctgtttt agtcgaatta ggagctcaag tttttagcat
     6181 tgaacgtcaa aaagtcctat ttcatcacac atattcgcta ttacctgaaa ttgggtatcg
     6241 tattcggatg tttcatggag atggctatgt gggattaccc aaatatgcac cgtttgacaa
     6301 aattatagtt actgctagtg cagaagaaat acctcaagaa ttgattcaac aacttaaaat
     6361 aggtggtatt atggtcattc ctgtaggaaa tacaatgttt aaagttacca aattaaccga
     6421 tacaacaaca gaaaaaattg aggtagacaa ttgtgctttt gtgcctatgc tacgtggaat
     6481 taattaatat attaacatga atattatcaa taaaatgaac gttaagacat ttatttttaa
     6541 cccttttcaa gaaaatacct atgtattgag cgacagcaac aataagtgca ttattattga
     6601 tgcaggttgc ctaaatttgg aagaaaaaaa tgatttgtat tcctacatta aagaaaataa
     6661 tttactcgtt gaacgtattt taaatactca tctacatatt gaccattgct ttggtaatca
     6721 atttgtttca gaaacattta ataaaaaaat tgaagctcat cccgatgatg aagctctatt
     6781 aaatggtatg aataaatatg ctcagacgtt cggaattgaa tatactgaaa atcaccctac
     6841 tatcagttat atttttgaag gagataccat tacagtaggt gaaatttcat taaaagcaat
     6901 acatgtgcca gggcattcaa aagggagctt agtcttttat gcagaaaaag aaaaatatct
     6961 ttttgcaggt gatgtattat ttagtggttc catcggaaga acagatcttc ctggaggaaa
     7021 ctttcacgat ttaattgata atattaagca aaaattactt attttgcccg atgaaacagt
     7081 tgttttttct ggtcatgggg ctcaaacaac catattgcac gaaaaacaat acaacccttt
     7141 tttataaaac gtaaaatatc atctcctcat aatatgtacc tccaaatcta atttccaatg
     7201 gtatgaaaat attcaactct acagctattc gccaaataga cgcttatacc attgaaaaaa
     7261 accacattaa atcaattgaa ttaatggagg tagctgctcg ttctgtattt gaatatatta
     7321 tacaacatta tgatatagaa aaaaaaataa tcatttttgc aggacaaggc aacaatggtg
     7381 gagatgcttt agccctagct cgtttgttac acatagcagg cttttcaatc aaagtaatta
     7441 ctgtgttttt atcggataat ttatcagatg attgtgaaac aaactataaa gcactactta
     7501 ataaaaatat acctatctac cattatagca gatacttaga actaaacata aaagaagatt
     7561 acttgattat tgatggcatt tttggcactg gactaaacaa accaataact ggtaaacttg
     7621 ctgacatgat tgtgtatatc aattcacgta attgcgaaat tatagccatt gatattcctt
     7681 ctggactacc ctctgatgga gttactgaaa ctaatcctat tgctattaaa gctactatta
     7741 ccattagttt tcaatttcca aagatatctt ttttctttgc cgagtacgaa tcatttatag
     7801 gaaaatggat acttactaat attggacttg ataaagaata tattgctaat aaagagacaa
     7861 atttctatac aattacgaaa gagagaattc agaagattct aaaaaaacgc tctgtatttt
     7921 ctcacaaagg cacttttggg cactgcctat taatgaatgg aagttatgga aaaatgggtg
     7981 ctggcatctt atcagctaag gcttgtttac gatctggagt aggcttgctc acaacacata
     8041 ttcctgtttg tggaaacacc gtaatgcaaa tagctgtacc agaagcaatg acatatacag
     8101 atatgcaaga acttacattg actagtgtac atgataacat caacgatttt gatgcagtag
     8161 gttgtggttg tggtattggt actgcaatac atactcaaaa aatgatacat aacttacttt
     8221 tatctaccaa taccccttgt gttttagatg ccgatgctct taactgtatt gcactaaata
     8281 actggaaaca tttattacct aaaaatacaa tcatcactcc tcaccctaag gaatttgata
     8341 gactggtagg cgaaagcaag cataatggtg aaagattaca aaaagccatc cttctaagca
     8401 aacaattaac tattgttatc atactgaaag gtgcatatac cagagtcgtt cttcccaatg
     8461 gaaaagtgta ttttaataca acaggaaacc caggtatggc aactgcgggt agtggagatg
     8521 tgcttacagg aattatagca ggattacggg cacaaaaata cgatgcaata gaggcatcct
     8581 tactcggagt gtaccttcat ggcttggctg gggattacgc taaagagaaa tattccgaaa
     8641 aatcacttgt tgcaacagat attattgaga acataagcga agcatacaaa tatatactat
     8701 agaaaatcgt attcgcaaat tataaagtac ccgaaatttc ttttaaccat tcgtgtgcaa
     8761 cggcatcact tggcatacgc caatcgcctc tcggagacaa acaaatactc cttactttag
     8821 gaccatcagg catacagtta cgtttaaatt gtt
//
LOCUS       DAOSRP010000083         8621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7798_length_8621_cov_5.136353, whole genome shotgun sequence.
ACCESSION   DAOSRP010000083 DAOSRP010000000
VERSION     DAOSRP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7798_length_8621_cov_5.136353"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..186
                     /locus_tag="PLJ82_06160"
     tRNA            103..186
                     /locus_tag="PLJ82_06160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Leu,seq:gag)
     gene            complement(295..1287)
                     /locus_tag="PLJ82_06165"
     CDS             complement(295..1287)
                     /locus_tag="PLJ82_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41126.1"
                     /translation="MFFNTSCKDKSRFFINTQQTSTPISIERFDKDIYSLQSGNYTNK
                     IDSLYHTYGSFVDIYTQHILEIGLFRNTGESIVTTNATDEIRKMLQDSMVKVLYQDCF
                     KEYRTTKDVEQTLNTAFGYFQHYFPNKQLPRVLFHFSGFHQSIVFTDSVVSASVEYYL
                     GPDYPIYQQVAYQYEIPTMNRKQLPIDIVHAWIKRYYEIETPHERLLDQLIYEGKIRY
                     LLSIFFPEYTEYEIMGYSKEQYEWSVKNEKNIWAYIIENKRLFSNNWREIAGFINPGP
                     FTSGLSQESPGRIGIWIGFRIVQQYAENNKDVTLEKLMEDNNSQRILELSGYRP"
     gene            complement(1328..1786)
                     /locus_tag="PLJ82_06170"
     CDS             complement(1328..1786)
                     /locus_tag="PLJ82_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOR41127.1"
                     /translation="MKTKLIISLLIIGLATACGTKKNNLSAAYEVATETQTEVQTETN
                     DTTTAKIATEQAMSDAKITETKDRYQMENGQFVEKPFHIIVGSFKNETNAKNLRNTLS
                     KNGMNAFVAVNKDGMFRVFAVSSDDEKTVRIELVKVRQTYPDAWILKLSK"
     gene            complement(1948..2862)
                     /locus_tag="PLJ82_06175"
     CDS             complement(1948..2862)
                     /locus_tag="PLJ82_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOR41128.1"
                     /translation="MKKYYIIALSLLFFCSSLFAQGEFRLSEQLFSRIKQNPSAIGND
                     KNLQVFNVNRMQWVGMGFDEAQKSTLINVHSYFESIQSGVGLSFSYDELGIANNTLNA
                     KLAYAYNIDLTENSLLSLGLGAGVINKTFNPSRHIWLHPTDATIPADVLSLTDLDIDF
                     GMELSTRYLLVGASANHLTNVGKEVSSLMAPTTMNAYARGLFAATNSIMIAPAVLYTN
                     IGALNQANFDVYQHNIVEVNATAFYNNQYWFGLGYRWNNALAFSAGLEWKKAFRLGYS
                     YELSVGDASQLTSSTHELMLSVVIPSKK"
     gene            complement(2871..4154)
                     /locus_tag="PLJ82_06180"
     CDS             complement(2871..4154)
                     /locus_tag="PLJ82_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR41129.1"
                     /translation="MKNLIITILLMSLASNLFAAIDTKFLEGYELQSVDINTPAKEHS
                     MSFLSKDSVVYLKGSEVFITTLNKEGNLSNPIASAPLQKLGIEGTVAFDSKLNKIYFS
                     KQDNKGNSELYESTFTNGVWSEPKVLFIEGLQKSRGSESFITNAGWSYAVAPIINFTN
                     PTIAKKGSRIYFTSSSIENGFGGTDIWYIDQKDEKTWLYPVNAGESINTTADEDFAFV
                     YNDTHLYFASKKEGNSFDIFKSEANGNTWTASTQLDAPYNLSGNDYNLIVKDGIPLLI
                     SDRNTGNGDDIFAFVKLPEPEPEPEPIPEPIPEPVKEFKWTLFLFDFDKDVLTDKFVA
                     ELDALAIQMKDFPEARFEISGYTDSRGSDKYNDALSLRRANRIKALLIEKGFKAEQFE
                     VVGNGKRKLNIPNASTEDEHAQNRRVEVKIINPIN"
     gene            complement(4235..6940)
                     /locus_tag="PLJ82_06185"
     CDS             complement(4235..6940)
                     /locus_tag="PLJ82_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOR41130.1"
                     /translation="MEKTFLKSKRFIRFLLVLCCFLGISASILAQNLTNKAIDKSASG
                     FGDLLGATTFSLADNNNATTQPNFTIGEIFSFPTGMSTIALNSIYTNGYGSWGTGGTN
                     TAPRYAIVSNPNVLNSGYKSITDGVNRLVMRTPNQSTEMFRFRVEGHKPGENVSVQFT
                     IQEITSNTVSGPTPQLVVNIKEVGGNDYNYYPKLAQGGTLNFSQTIDRNSGAIEVIVS
                     RHSWDDGANFVYAISNIKVYGTSYQYIDALNSTVCDGDNVTLTAEGFEAFSGDYTWES
                     SSTETGPFTTIAGEISSTINVDALLGNTFYKVTRGTATSEVFRLNARVCCKETAGQQI
                     IFKESFGNVTSGTRKNFADIGTTGIVPLTYKATGNVNDGQYAIVSKTYDAEDFSVGGG
                     HPWYWAGTDHTGNTNGGMLFINCGNNIKNKPVYVRTLDGAQLCNSTYLFFSVYMANPA
                     KTGGAPSRFRLEIWGYNSPSDSTLIESLLTGDIIAGDTQWKQFGTSFLPGTYPELKVK
                     VISLSDAITGNDVIIDDISVSVCAPSIAMGVNGNPSITETSVTCGSSATLDVISLTDL
                     SKLFTSTPYYRWEQSIDDGVSWTETTHSGIGVITATVSQNDETTPTIYRVIIAEDATS
                     AETISDGTDGTALSDVCATFGITNNIKVSCTSSNPVPVITLATDKTIICGGQENATLT
                     VTVTAGNVASCIWKYSVDEGTTWMDMTETGKSIQVSPTVKTSYQVTGLNENDLPSNTE
                     TVVVDVTPQVTVSLATSTAKVILGGTATLTATTTPSSNTLAWTVNGNSLAATNPHTVS
                     PYLTERYQVTATDGCTSATSELVEIKVEWPTAFNPYNQDGLNDTFANGFTIIVFDRYG
                     KAIFEGANGWNGKVDGMFAQPAVYYYVVTLPNNQVKKGTIEVVKP"
     gene            7222..8103
                     /gene="fabD"
                     /locus_tag="PLJ82_06190"
     CDS             7222..8103
                     /gene="fabD"
                     /locus_tag="PLJ82_06190"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOR41131.1"
                     /translation="MKAFVFPGQGAQFVGMGKDMYENSALAKEMFEKANEVLGFRITD
                     LMFEGTDEDLRQTKVTQPAVFLHSVILAKTMGDKFAPDMVAGHSLGEFSALVAAGALS
                     FEDGLVLVSKRAMAMQKACEIEPSTMAAVLALDDAKVEEICASINGVVVAANYNCPGQ
                     LVISGSIAAVEEACANMLEAGAKRALKLPVGGAFHSPLMEPARVELAAAIEKAQIVAP
                     ICPIYQNVTASAETDPKVIKAKLVEQLTAPVRWTQSVQNMIADGATLFVEVGPGKVLQ
                     GLVKKINKDSQVQSIED"
     gene            8414..>8621
                     /locus_tag="PLJ82_06195"
     CDS             8414..>8621
                     /locus_tag="PLJ82_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41132.1"
                     /translation="MCSAKSNSYNFSLKAFYQIFLFLFLFSTLPIFSQGVGQYPVQVS
                     TVVLPNPPMYLSAYSGVETDKLSVT"
BASE COUNT         2588 a         1513 c         1527 g         2993 t
ORIGIN      
        1 agagcataac cttgccaagg ttagggtcgc gggttcgagt cccgtttttc gctctctaaa
       61 aatgcgaagc gtagaggttt cgcattttct atctgaaata ttgcacaagt ggtgaaattg
      121 gtatacacgc tactttgagg tggtagtggg cgaaagcctg tgtgagttcg agtctcatct
      181 tgtgcacaaa aaagaggcag tcttaaaata ttttaagact gcctcttttt attagggtat
      241 ttgatgaaga atatattctt agcggtttaa aagtttattc agacagcttc ttttttatgg
      301 tcggtagcca gataattcta aaatacgttg gctattgttg tcttccataa gtttctctaa
      361 ggttacatcc ttattgtttt cagcatattg ttgcacaata cggaaaccta tccaaatccc
      421 aatgcgtcct ggcgattctt gagagagtcc agaagtaaaa ggaccaggat taataaatcc
      481 agcaatttca cgccaattat ttgaaaacaa tcgtttattt tcaataatat atgcccaaat
      541 atttttttca tttttcacac tccattcata ttgctcttta ctatatccca ttatttcgta
      601 ttctgtatac tctggaaaga atatggataa aagatacctg attttgcctt catagattaa
      661 ctgatcaagt aaacgttcat gtggcgtttc tatttcataa taacgtttaa tccatgcatg
      721 tacaatgtcg attggtaatt gtttccgatt cattgtcgga atttcatatt gataggcaac
      781 ttgttggtaa ataggatagt ctggtcctag atagtattca acgctggctg atacaacact
      841 atctgtaaaa acaatagatt gatgaaagcc cgaaaaatgg aaaagtacac gtggaagctg
      901 tttgttagga aagtaatgct gaaaataacc aaatgccgta ttcagtgttt gttccacatc
      961 tttcgttgta cgatattcct taaaacaatc ttggtaaagt acttttacca tactgtcttg
     1021 gagcattttt cttatttcat cagttgcatt tgtggtaaca atgctttctc ctgtgtttct
     1081 aaaaagacct atttctaaaa tatgttgggt gtaaatatcc acaaatgaac catatgtatg
     1141 atataagcta tctattttat ttgtgtaatt acctgactgg agcgaataaa tatctttatc
     1201 aaaacgttca atagaaatcg gcgttgaagt ctgttgtgtg ttgataaaaa acctactttt
     1261 gtctttacag cttgtgttaa agaacaaaag taggagtata atataaaagt ttaaaaaaat
     1321 cctcaactta tttgcttagc tttaaaatcc atgcatctgg atatgtttga cgtactttta
     1381 ctaattcaat acgaacggtt ttttcatcat ctgatgaaac agcaaatacg cgaaacatgc
     1441 catctttatt tacagctaca aaagcattca tgccgttttt agagagagtg ttgcgtaggt
     1501 tttttgcatt tgtttcgttt ttgaaactac caacaataat gtggaatggt ttttcaacga
     1561 attgaccatt ttccatttgg taacggtctt ttgtttcggt aatttttgca tcagacatgg
     1621 cttgttctgt tgctattttt gcagttgttg tatcatttgt ttctgtttgt acttctgttt
     1681 gtgtttcggt tgccacttcg taagctgctg ataggttgtt ttttttagtt ccgcaagctg
     1741 tagctaatcc aatgataagt aagctgataa taagttttgt tttcatgtgt gtgctgtttt
     1801 tttttatttg tataaaacga tgttaaaaat caccacaaat atctgcattt tttttcatac
     1861 aaaaaaacct ataagttttt ttaagaaaaa aagtctgttg tattcatcac aacagacttt
     1921 tttgattgtt aattaaacgt ttgatcgtta ttttttacta ggaataacca cagaaagcat
     1981 taattcgtgt gtactagaag ttaattgtga ggcatctcca acactcaatt catatgagta
     2041 gccaagacgg aaggcttttt tccattccaa tccagctgaa aatgctaggg cattgttcca
     2101 tctatatcct aaaccaaacc aatattgatt gttataaaac gctgttgcat ttacttctac
     2161 aatgttgtgc tgatatacat caaaatttgc ttgatttaat gctccaatat tggtgtatag
     2221 tactgcagga gcaatcataa tactatttgt tgcagcaaat aaaccacgag cataagcatt
     2281 cattgttgta ggagccatta atgacgaaac ttcttttcct acattggtta aatgattagc
     2341 agaagcacct accaataaat acctagttga taattccata ccaaagtcga tatccaaatc
     2401 agttaatgac agtacatcag caggaattgt tgcgtctgta ggatgtaacc aaatatgtct
     2461 acttggatta aatgttttgt taataacacc tgcacctaga cctagtgata ataaactgtt
     2521 ttcagttaag tctatattgt aagcatacgc tagtttggca tttagtgtat tgttagcaat
     2581 acccaattca tcgtaagaaa aagataatcc aactcccgat tgaatgcttt caaaataaga
     2641 atgaacattg ataagagttg atttttgtgc ttcgtcaaac cccattccta cccattgcat
     2701 acgatttaca ttgaaaactt gtaagttttt atcgttacct attgcagatg gattttgctt
     2761 gatacgggaa aataattgtt cagaaagcct gaattcacct tgagcaaata gtgagctaca
     2821 gaagaatagc aacgataacg ctattatata atattttttc atgttattca ttagttaata
     2881 gggtttataa ttttaacttc tactctacgg ttttgagcat gttcatcctc agttgatgcg
     2941 ttcggtatat ttaactttct ttttccgtta ccaaccactt caaactgttc agctttaaat
     3001 cctttttcta ttaacaatgc ttttattcgg tttgctctac gcaaagataa tgcatcattg
     3061 tatttgtctg aacctctgct gtcagtatat ccagatattt caaaacgagc ttctgggaag
     3121 tctttcattt gtatagccaa tgcatctagc tctgctacga atttgtctgt taatacatct
     3181 ttatcaaaat caaacaagaa caatgtccat ttgaactctt ttacaggttc tggtattggc
     3241 tctggtattg gttctggctc tggctcaggt tctggaagtt ttacaaaagc aaaaatatcg
     3301 tccccattac cagtgtttct atccgaaata agcaatggaa tgccatcttt tacaattaag
     3361 ttataatcat ttccagaaag attatatgga gcatctaatt gagttgatgc agtccacgta
     3421 tttccattag cttctgattt aaaaatatca aaagaattac cctctttttt agaggcaaaa
     3481 tacaaatgag tatcgttata tacaaaagcg aaatcttcat cagcagtcgt attgattgat
     3541 tctcctgcat ttacaggata caaccatgtt ttttcatctt tttggtcgat ataccaaatg
     3601 tctgttcccc caaatccgtt ttcaatagaa ctagaagtga aataaatgcg actccctttt
     3661 tttgcaatgg ttggattcgt aaaattaata ataggtgcca ccgcataact ccatcctgca
     3721 ttggtaataa atgattcact tccgcgagat ttttgcaatc cttcaatgaa aagaacttta
     3781 ggttcagacc atacgccgtt tgtaaatgtt gattcgtata gttcgctatt tcctttgtta
     3841 tcttgttttg agaagtaaat tttattcaac ttactatcaa atgccacagt tccttctata
     3901 cctagttttt gtaatggggc agatgcaata gggttgctta gatttccttc tttatttaat
     3961 gttgtaataa atacttcact tccttttaaa taaacaacag agtctttaga aagaaaagac
     4021 atgctatgtt ctttcgctgg tgtatttata tcaacagatt ggagttcgta tccttcaagg
     4081 aactttgtgt cgattgctgc aaatagattg ctagccaaac tcattaaaag aattgttata
     4141 attaaatttt tcatatatct acttttttat tattgttgta tgatacttgc caagtaatac
     4201 ccagtagttt tacttggctc gtttcattca tattttatgg ttttactact tcaatggtac
     4261 cttttttaac ttgattatta ggaagtgtaa ctacataata ataaactgca ggttgagcaa
     4321 acataccgtc tactttgcca ttccatccat ttgctccttc aaaaatagct ttaccatatc
     4381 tatcaaaaac gatgatagta aatccattag cgaacgtatc attcagccca tcttggttgt
     4441 atggattgaa agcagtaggc cattcaacct tgatttctac taattctgaa gtagccgaag
     4501 tacaaccgtc agtagcagta acttggtatc tttcagttag ataaggacta actgtatgtg
     4561 gattagttgc tgctaaagaa tttccgttta ctgtccaagc aagcgtattg ctagaaggag
     4621 ttgtggttgc tgtgagtgtt gcggtacctc cgagtattac ttttgcagtg ctagtagcta
     4681 acgatactgt tacttgtggt gtaacatcta ctactacagt ttctgtattt gatggtaaat
     4741 cattttcgtt gagtccagtt acttggtagc ttgtttttac agtaggagat acctgaatac
     4801 tttttcctgt ttcagtcata tccatccatg tagtaccttc atctactgaa tacttccaaa
     4861 tacaacttgc tacattgcca gctgttacag ttacagttag tgttgcattt tcttgaccac
     4921 cacaaatgat tgttttatca gttgctaatg taattacagg tacagggtta cttgatgtgc
     4981 aagatacttt aatgttattg gtaatcccaa atgtagcaca tacatctgat aatgctgtac
     5041 catctgtacc atccgaaata gtttcggcac tagttgcgtc ttctgctata attactctgt
     5101 aaatagtagg ggtagtttca tcgttttgcg aaacagtggc agttataacg ccaataccac
     5161 tatgtgtagt ttctgtccaa cttactccat cgtcaatact ttgttcccat ctatagtaag
     5221 gagtagatgt aaataattta gataagtccg ttaatgatat cacatccaat gtggcagaac
     5281 ttccacatgt tacagaagtt tcagtaatac ttggatttcc atttactccc atagctatac
     5341 ttggggcaca aacagacacc gaaatatcat caataattac atcattccct gttatggcat
     5401 cacttaacga aataactttt acttttagtt ctgggtaagt gccaggaaga aaggatgttc
     5461 caaattgttt ccactgtgta tccccagcaa taatatctcc agttaatagg ctctctataa
     5521 gtgttgaatc ggaaggtgaa ttgtatcccc agatttctaa acgaaaacga gagggagctc
     5581 ctccagtttt tgcagggtta gccatataca ctgagaaaaa taaataagta ctattacata
     5641 actgagctcc atctagtgtt ctaacgtata caggtttgtt tttaatgttg tttccacaat
     5701 ttataaaaag catacccccg tttgtatttc cagtgtgatc tgtacctgcc cagtaccatg
     5761 gatgaccacc accaacgcta aagtcttctg catcgtatgt ctttgacaca atggcatact
     5821 gtccgtcatt cacatttcct gtggctttgt atgttaatgg aacaatacca gtagttccta
     5881 tgtcagcaaa attctttctt gttccactag taacattgcc aaagctttct ttaaaaatta
     5941 tttgttgtcc tgctgtttct ttacagcata cacgtgcatt taatcgaaat acttcagaag
     6001 tagcagttcc tctagttact ttataaaagg tattgcctaa taatgcatca acattaatag
     6061 tagaggaaat ttcacctgca atagttgtaa aaggacctgt ttctgttgaa gacgattccc
     6121 atgtataatc accgctgaaa gcctcaaacc cttctgctgt aagtgtaaca ttgtctccat
     6181 cacatacagt agaattaagt gcgtctatat attgatagga tgtaccataa accttaatgt
     6241 tgctgatagc gtacacaaaa tttgcaccat catcccagct atgtctacta acaattacct
     6301 caattgcccc actgtttcta tcaatagttt gcgaaaaatt taatgtgcct ccttgtgcta
     6361 acttaggata gtagttgtag tcatttccgc ctacttcttt tatgtttacc actaattggg
     6421 gagttggacc actaaccgta ttgcttgtta tttcttgtat agtaaattgc acactaacgt
     6481 tttctcctgg tttatgtcct tctactctaa agcgaaacat ttcggttgat tgattaggcg
     6541 tacgcataac caaacgattt acaccatccg taattgattt gtagccacta ttcaatacat
     6601 ttggattgct aacaatagca taacgtggag ctgtatttgt gcctcctgta ccccaactgc
     6661 catacccatt tgtgtatatg ctgtttaatg caatagttga cattcccgtt ggaaaagaaa
     6721 atatttcccc gatggtaaaa ttgggttgtg ttgtagcatt gttattatct gctaatgaaa
     6781 acgtggtagc tccaagtaaa tcgccaaaac cagatgcact tttgtcaatg gctttgttgg
     6841 taagattttg agctaagatg cttgctgata tgcccaaaaa gcaacataag acaagcaaaa
     6901 aacgaataaa tcgtttggat tttaggaatg ttttttccat aataaaatca agtttaggtt
     6961 aaaagaattt gtaataattt gctaacaagc tatttatgtt tagaataagg tgcaaagtaa
     7021 gtacaagttt ttgtatttaa caagcttttt ttatgttttt tttgagtatg atgataaaaa
     7081 gagtattttt gcactcgaaa aatttacata cgttaattag cattatctga tgcatttgct
     7141 ctagctttat tcttagctaa aaatgtagaa ttgcagatga aaaatgctgt ttttttagtt
     7201 gtttaattta aaataatcaa aatgaaagca tttgtatttc ctggtcaagg agctcagttt
     7261 gtaggaatgg gcaaagatat gtacgaaaat tcagctttag cgaaagagat gtttgaaaaa
     7321 gcaaacgaag tattaggttt tcgcattacc gatttgatgt tcgaaggaac agatgaggat
     7381 ttgcgtcaaa ccaaagttac gcaacctgcg gtatttttac attcagttat attagccaaa
     7441 accatgggcg ataaatttgc tcccgatatg gtagcgggtc attcgttggg agagttttct
     7501 gctcttgtgg ctgctggtgc attgagtttt gaagatggct tggtattggt gtcgaaacgt
     7561 gcaatggcta tgcaaaaagc gtgcgaaatt gaaccatcga ccatggctgc ggtattggca
     7621 cttgatgatg ccaaagtgga agaaatatgt gcttcaatca atggcgtagt ggtagctgct
     7681 aattataatt gtccaggaca gttagttatt tctggttcta tagctgcggt agaagaagcg
     7741 tgtgccaaca tgttggaagc tggtgcaaaa agagccttaa aattacctgt aggtggtgcc
     7801 tttcattcgc cattaatgga gccagcacga gtagaattgg ctgcggcaat agaaaaagct
     7861 cagattgtag ccccaatatg tccgatatac caaaatgtaa cagcttcggc tgaaactgac
     7921 ccaaaggtaa ttaaagccaa attagtagag caacttacag cacctgttcg ctggacacaa
     7981 tctgtacaaa atatgattgc cgatggagca accctctttg tagaagtagg accaggaaaa
     8041 gtattgcaag gtttggtgaa aaaaataaac aaagatagcc aagtacaatc gatagaggat
     8101 taagagggct tgttgtagct atagcttgtg taaagaatag agtttctttt gcttgaatta
     8161 tttacctata gttagcttat tctcaaattt tattattgaa tagttattga tttttcgctt
     8221 acacgatgat tccattgtgt aagcgtgttt tttatctttt taaaaggagg caatctattt
     8281 ttcaagaaac atcgtattct cataatatgg tttgaatgat taattatatg taaaatatcg
     8341 tttttacgta taaaaacata atgtgaacat agacaataat aaaaagtaga taattaattg
     8401 aatagacgaa gtaatgtgtt ctgcaaaatc aaactcatat aatttctcgt taaaagcatt
     8461 ttatcaaata ttcttattcc tatttttatt ctccacgctc cccatatttt cacagggtgt
     8521 aggacaatat cctgttcagg tcagtacagt agtattgccc aatccaccga tgtatttatc
     8581 cgcatacagt ggagtagaga ccgataaact atccgttacc t
//
LOCUS       DAOSRP010000084         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7804_length_8612_cov_5.865140, whole genome shotgun sequence.
ACCESSION   DAOSRP010000084 DAOSRP010000000
VERSION     DAOSRP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7804_length_8612_cov_5.865140"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLJ82_06200"
     CDS             <1..384
                     /locus_tag="PLJ82_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOR41133.1"
                     /translation="YAGPAKTPQGMACGSMGPTTAGRMDSYVDLFQSQGGSMIMLAKG
                     NRSQAVTDACQKYGGFYLGSIGGPAAILAQNNIKKIECIEYPELGMEAIWKIEVEDFP
                     AFILVDDKGNDFFKQLKPGCVKALK"
     gene            complement(449..1582)
                     /locus_tag="PLJ82_06205"
     CDS             complement(449..1582)
                     /locus_tag="PLJ82_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOR41134.1"
                     /translation="MSVSIKEVVSRKDLKNFVQFGTDLYHDSPYFCPPLFFDEINTLD
                     KKKNSAFEVCEAIYYLAYKNNKIVGRIAGIVNHRANEAWGVKKTRFGWFDFIDDTDVS
                     FSLLKAVENWGKAKGMDSINGPVGFTDFDHEGLLIEGFDQLGTLATIYNYPYYAKHYE
                     AYGLTKDIDSLEWKIYTPKEVPERMARISALVKEKYNLRYVKVKSHKELVKRFGYTFF
                     DLIDEAYRPLYNYSPLTESQKKYYCEIYFPMLNFDFLSLVVNDKDELVALGVSMPDIT
                     KALQKCRGKLFPFGFIHILKGLKAKKIDVIDLMLIAVRPDYQNKGVNSLLFDEQTPYY
                     NKYQVKYSESSPVLETNLKSQANYAYFETEQHKRRRIYIKPIV"
     gene            1785..2747
                     /locus_tag="PLJ82_06210"
     CDS             1785..2747
                     /locus_tag="PLJ82_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR41135.1"
                     /translation="MINKVLLTGANSMLGVNTALLLLAKGYEVKALLRNSREELNACN
                     IIHGDITKFEDVKDAAIGCDAIIHIAAATNQEMLHLSDYTKINVVATENVCQASLVTS
                     AKRVIFVSTANTIGNGTKEKPANETHPANKPFADNLYAKSKLLAEEKALASGANILII
                     NPTFMIGAYDSKPSSGQIITMNYGKRIVFAPKGGKNFVAASDVANAIVNALSSTMIGE
                     KILTGGYNLSFADFYKTMAQTQGYKISVINLSNSLLYAVGIIGSLCRKFGIKTPVSFT
                     NMRILCTREWYTAEKAIHALQMPQTSIEKAITDCINWFKANNML"
     gene            2753..3673
                     /gene="queG"
                     /locus_tag="PLJ82_06215"
     CDS             2753..3673
                     /gene="queG"
                     /locus_tag="PLJ82_06215"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOR41136.1"
                     /translation="MNKQQLTDTIKQQALTLGFDAVGIAPSQLLEQDKEYLIKWLNKG
                     YAASMKYMHTHLEKRVNPASLLENTRSVIVVLYHYLPTTQQASQQYKIAKYAYTPDYH
                     YVIKSKLQQLASLLPTENADIQRWFTDSAPVLERRWAQLAGLGWIGKNGCFIHPQLGS
                     YVFIGEIFTEHELVYDTPYTQEHCGTCTRCLQACPTQALIAPRELDANKCISYHTIES
                     KNEIPSSIAQQLKGYIFGCDICQDICPWNAKIVAKEKKDSPTMPYVYYSDQDWLTLDK
                     KKFKQDFRHSPMQRAGYVKLIKNIRTVVEK"
     gene            3834..5114
                     /gene="metK"
                     /locus_tag="PLJ82_06220"
     CDS             3834..5114
                     /gene="metK"
                     /locus_tag="PLJ82_06220"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOR41137.1"
                     /translation="MGYLFSSESVSEGHPDKVADQISDALLDQFLAQDASSKVACETL
                     VTTGQIILAGEVKSEAYVDLQKTARNVVKRIGYTKSEYMFDADSCGVLSAIHEQSPDI
                     NRGVERTNPDDQGAGDQGLMFGYACNETKNLMPIALDLSHALLKELAAIRKEGKVMTY
                     LRPDAKSQVTIEYGDDHKPKRIHTIVVSTQHDDFLTPTDTLTQEEADKQMLETIYNDV
                     KNILLPRVIAKDEQYASLFHNNIVLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYATRYIAKNIVAAGIADEILVQVAYAIGVAKPMNIYVN
                     TYGSAKVNKSDAEIASIIETIFDLRPAKIEHMLALRNPIYQETAAYGHMGRTPRTITK
                     EFEISGGKKISQTVELFTWEKLDKVDTIKKAFGL"
     gene            5133..6119
                     /gene="dusB"
                     /locus_tag="PLJ82_06225"
     CDS             5133..6119
                     /gene="dusB"
                     /locus_tag="PLJ82_06225"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOR41138.1"
                     /translation="MKIANVEFPPYPLFLAPMEDVTNPSFRLLCKQYGADMVYTEFIS
                     ADALIRNVQRTEQKLHISQDERPVAIQLYGREPDAMAEAARIAETAQPEIIDLNFGCP
                     VKKVAGKGAGAGLLRDIPKMLAITKSVVNAVSIPVTVKTRLGWDDSSRIIVELAEQLQ
                     DCGIAALTIHGRTRAQMYTGNADWQLIGEVKNNPRMHIPIIGNGDVTTPERTKECFDK
                     YGVDAVMVGRACIGKPWIFQEIRHYLQTGEHIPQIPFTEQLSILKDQVERSIEWLDER
                     RGILHIRRHLAATPLFKGIPNFKDTRIAMLRATTKDELFAIFNAIETMVVER"
     gene            6251..>8612
                     /locus_tag="PLJ82_06230"
     CDS             6251..>8612
                     /locus_tag="PLJ82_06230"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOR41139.1"
                     /translation="MNKENQIEENLIEQLKGLKYIHRPDIVDRKTLEQNFKAKFEELN
                     RVHLSDNEFLRLREEIINSDVFFASKKLRERQYFQREDGTPLHYTLVNIKDWCKNDFE
                     VISQLRINTENSHQRYDIILLINGLPVVQIELKRGDISHRKAMQQIVDYKNEPGNGYG
                     NSLLCFMQLFIVSNSGRTLYFANNKNQHFAFNADEQFLPVYELADIDNKKINGLFDFT
                     DKFLSKCTLGEMISKYMVLVESEQKLLVMRPYQIYAVKAIEECVKQNRGNGYIWHTTG
                     SGKTLTSFKASTLLKDNHEIEKCLFVVDRKDLDRQTREEFNKFQEGSVEENTDTETLV
                     KRLLSMDYADKVIVTTIQKLGLALDGTYKKNYKERLEPLSKKRVVFIFDECHRSQFGE
                     NHKAIKEFFPNAQLFGFTGTPIFDENATYSIREGEFASYKTTEDIFQQRLHSYTITHA
                     IDDKNVLRFHIDYFKGNKNQKPGEAISRQAVVDAILNKHNAATNSRKFNAVLATASIN
                     NAIEYYELFKELQKKKQKENPEYVPLNIACVFSPPSQLIATQGDQQSQKNAADIKQLQ
                     EDLTQEREDNKQNPEEKKKALTEIIEDYNKQFNTNHSINEFDLYYQDVQRRIKDQQYS
                     NKDYPHENKIDITIVVDMLLTGFDSKYLNTLYVDKNLKYHGLIQAFSRTNRVLNDTKP
                     YGNILDFRSQQESVNQAIALFSGEDNGKAREIWLVDPAPVMIDKYKEAVEKLGVFMQE
                     HNLVNEPQEVYNLKGDAARIAFVQNFKEVQRLKTQLDQYTDLDEQQK"
BASE COUNT         3001 a         1579 c         1615 g         2417 t
ORIGIN      
        1 tatgctggac cagcaaaaac gcctcaaggc atggcgtgtg gttcgatggg tcccactaca
       61 gctggacgta tggattcgta tgtggatttg tttcagtcgc aaggaggcag catgattatg
      121 ttggcaaaag gaaaccgcag tcaagcagta actgacgctt gtcaaaaata cggcggtttt
      181 tatcttggtt ctatcggcgg accagcggct attttagctc aaaacaacat caaaaaaatt
      241 gaatgcatcg aatatcctga acttggaatg gaagctattt ggaaaattga agtagaagat
      301 tttcccgctt ttatcttggt tgatgacaaa ggaaatgatt ttttcaaaca actgaaacct
      361 ggttgtgtaa aggctctgaa ataatagcat ttcattagac aaaaaaatgg gagaaatcaa
      421 cgaacgattt tctcccattt tttgtttatt aaactatcgg cttaatataa atacgacgac
      481 gtttgtgttg ttcggtttca aaataggcgt aattggcttg actctttaag tttgtttcca
      541 acacaggcga tgactccgaa tattttactt gatatttatt gtaataagga gtttgttcat
      601 caaataacaa cgaatttacc cctttgtttt gataatctgg acgtactgca attaacatca
      661 aatcaattac atcaattttt ttagctttta acccctttaa tatatggata aaaccaaatg
      721 ggaataattt acccctgcat ttttgtaagg ctttggttat atcaggcata ctcactccta
      781 gtgcaaccaa ttcgtcttta tcattcacta ccaaggatag aaaatcaaaa ttaagcatag
      841 ggaaataaat ttcgcaatag tatttttttt gactctccgt gagtggagaa taattgtata
      901 agggacgata cgcctcatct attaaatcaa aaaatgtgta tccaaaacgt tttaccaatt
      961 ctttgtggct ttttaccttg acatagcgta aattgtattt ttcttttacc aaggctgaaa
     1021 tacgtgccat acgttcgggc acttctttgg gcgtataaat tttccattca aggctatcaa
     1081 tgtctttggt taagccatac gcttcgtaat gctttgcata atacggataa ttataaatag
     1141 tggcaagtgt ccctagttgg tcaaatccct caataagtaa accttcgtgg tcaaagtcag
     1201 taaatccaac aggtccattg atgctatcca tccctttggc ttttccccaa ttttcaacag
     1261 cttttagtaa actaaacgat acatctgtat catcgataaa atcgaaccaa ccaaaacgcg
     1321 tttttttaac tccccaagcc tcattggctc tatgattaac tataccagct atgcgaccaa
     1381 caattttatt atttttatag gctaaataat aaattgcctc acacacttcg aaagcagaat
     1441 tcttcttttt atcaagggta ttgatttcat caaaaaataa aggtggacaa aaatatgggc
     1501 tatcgtggta taaatcagtt ccaaattgaa caaaattctt caagtcttta cgtgaaacaa
     1561 cttctttaat acttacagac atatcgttta attttaaggt aaattgagtg caaagttaca
     1621 aaaggatttt aatttatagt tatttttgct ttcttttttt gagctacaat tgataacata
     1681 ttatttttca attatttata ttgtgtgtca tgtttcaata aattgcattg aaaatccaaa
     1741 tatgtttagt atttttacgc tgttttcaag aatatatcaa acacatgata aacaaagtac
     1801 tgcttacagg agcaaacagt atgcttggcg taaatacagc attactgtta ttagcaaaag
     1861 gctacgaggt gaaagcatta cttcggaact ctcgagaaga acttaacgcc tgtaacatta
     1921 ttcacggaga cattacgaag tttgaagatg tgaaagatgc tgcaataggt tgcgatgcca
     1981 ttattcatat tgcagcagct accaatcaag aaatgttgca cctatctgat tacacaaaaa
     2041 tcaatgtcgt tgcaaccgaa aatgtctgtc aggcatcact agtaacaagt gccaaaaggg
     2101 ttatcttcgt aagtactgcc aataccattg gtaatggaac caaagaaaag ccagccaatg
     2161 aaacgcaccc agcaaataaa ccttttgcgg ataatttata tgccaaaagt aaactgttgg
     2221 cagaagaaaa agccttagca agtggtgcaa atattctcat catcaatcct acttttatga
     2281 ttggtgcgta tgatagcaag ccaagttcag gacaaataat taccatgaat tatggtaaac
     2341 ggattgtgtt tgcccccaaa ggaggtaaaa attttgtggc agcaagcgat gtggcaaatg
     2401 ccattgtaaa tgcactcagt tcaactatga taggagaaaa aattcttact ggtggataca
     2461 atctttcttt tgccgatttt tataaaacaa tggctcaaac acaaggctac aaaataagcg
     2521 ttatcaactt atccaacagt ctgttatatg ctgtaggtat cattggttca ctttgtagaa
     2581 aattcggaat taaaacaccc gtttcgttca ccaatatgcg tatattgtgt acaagagaat
     2641 ggtacactgc agaaaaagcc atacacgcac tacaaatgcc acaaacgtct attgaaaaag
     2701 caattaccga ttgtatcaat tggtttaaag caaataatat gttgtaatgg ttatgaacaa
     2761 gcaacaactc actgacacca tcaaacaaca ggctcttaca ttgggctttg atgcggtggg
     2821 aattgctcct tctcaattat tagagcaaga caaagagtat ctcatcaagt ggctaaacaa
     2881 aggctatgcc gcctcaatga agtacatgca tacacatctt gaaaaaagag taaacccagc
     2941 ctcattgctc gaaaatacac gttcggtgat tgtcgttttg tatcactatt tacctactac
     3001 ccagcaagcc agtcagcaat acaaaatagc aaaatatgct tatactcctg attatcatta
     3061 tgttataaaa tctaagctac agcaattagc atcactatta ccaacagaaa atgctgatat
     3121 tcaacgttgg tttaccgact cggctcccgt attagaaaga cgttgggcac aactagctgg
     3181 actgggttgg attggtaaaa atgggtgttt tattcatcct caattaggct cgtatgtgtt
     3241 tattggagaa atatttaccg aacacgaatt ggtgtacgac actccctaca cgcaagaaca
     3301 ttgtggcaca tgtacacgtt gtttacaagc atgtcctacg caagccttaa tagctccacg
     3361 agaactagat gccaataaat gtatttcgta ccacacgata gaaagtaaaa atgaaattcc
     3421 ttcctctatc gcccaacaac tcaaggggta tattttcggg tgtgatatat gccaagatat
     3481 atgcccatgg aatgctaaaa ttgtggcgaa agaaaaaaag gattctccca caatgcccta
     3541 tgtgtattat agcgaccaag attggttaac gctggataaa aagaagttta aacaggattt
     3601 ccgacactct cctatgcaaa gagcgggata tgttaaacta ataaaaaaca tacgaacggt
     3661 agttgaaaaa taaaaaataa cttctacctt tgtaccatca aagtggatgg atttggatag
     3721 cttgcaacca cagaaaaaaa tgctaaaaca aaaaacatac ttttctgcct ttttcgatat
     3781 ccaaaactca aatcactttt attattttat aacaaactaa taattaaagt attatggggt
     3841 atttattttc atcagaatca gtttctgaag ggcatccaga caaggttgcc gaccaaattt
     3901 ctgacgcact gctcgatcaa tttttagcac aagatgcgtc ttcaaaagta gcatgcgaaa
     3961 ccttagttac aactggacaa attattttgg ctggtgaagt aaaatcagaa gcgtatgtag
     4021 atttacaaaa aacagcacgc aatgtggtta aacgtattgg ctacaccaaa agcgaataca
     4081 tgtttgatgc cgattcgtgt ggtgtattaa gtgctattca cgaacaatcg cctgatatca
     4141 atagaggcgt agaaagaacc aatcctgacg atcaaggtgc aggagaccaa ggattaatgt
     4201 ttggttatgc ctgcaacgaa acaaaaaacc tgatgccgat agcattggat ttatctcacg
     4261 ccttgctcaa agaattagca gctatacgca aagaaggtaa agtaatgaca tacttgcgtc
     4321 cagatgctaa atcgcaagtt actattgaat acggtgacga ccataaacca aaacgcatac
     4381 ataccattgt tgtatctact caacacgatg attttttaac accaaccgat acacttacac
     4441 aagaagaagc ggacaaacaa atgttagaaa ccatctataa tgatgtgaaa aatattttac
     4501 ttccacgcgt tattgccaaa gacgaacaat atgcctcatt atttcacaac aatattgtgt
     4561 tgcatgtgaa tccaacaggt aaatttgtaa ttggcggtcc acacggtgat acaggtttaa
     4621 caggtagaaa aattatcgta gatacatacg gtggcaaagg agcacatggt ggtggtgctt
     4681 tttctggaaa ggacccctca aaagttgacc gctctgctgc ctatgctaca cgctatattg
     4741 ccaaaaatat tgtggcagct ggcattgctg acgaaatttt ggtacaagtt gcttatgcca
     4801 ttggtgtagc aaaacctatg aatatttatg taaacacgta tggttcagcc aaagtaaaca
     4861 aatcagatgc cgaaatagcg agcattattg aaacaatctt tgacctacga cctgcaaaaa
     4921 ttgaacacat gttagcttta cgcaacccca tataccaaga aactgcagct tacggacaca
     4981 tgggacgcac accacgcacc ataacgaaag aatttgaaat atctggtggc aagaaaatca
     5041 gccaaacagt agaactattt acttgggaaa aacttgataa ggttgacacg ataaaaaaag
     5101 catttggttt gtaatccatt tacttgtaca ccatgaaaat tgctaacgta gaatttcctc
     5161 cttatccctt atttttggca cctatggagg atgttaccaa ccctagcttt aggttgttgt
     5221 gtaagcaata tggtgcagac atggtatata ccgaatttat atcggctgat gcgttgatac
     5281 gcaatgtgca acgaacagaa caaaaattgc acatcagcca agacgaaaga ccagtggcta
     5341 ttcaactata cggcagagaa cccgacgcta tggcagaggc tgcacgtata gctgaaacag
     5401 cacaacccga aattattgat ctgaattttg gttgtccagt aaagaaagtg gcaggcaaag
     5461 gagcaggtgc aggtttgcta cgtgatattc cgaaaatgtt ggcaattacc aaaagtgttg
     5521 tcaatgcagt atctattcct gtaactgtaa aaactcgcct tgggtgggac gatagtagcc
     5581 gtattattgt agaattggca gagcaattac aagattgtgg catagcagca ctcaccattc
     5641 atggacgcac acgggcacaa atgtacacag gtaatgccga ctggcaacta attggcgaag
     5701 tgaaaaacaa cccccgcatg catatcccca ttattggtaa tggtgatgta actaccccag
     5761 aaaggacaaa agagtgtttt gataaatacg gtgtagatgc ggttatggta ggacgtgcgt
     5821 gcattggcaa accttggatt tttcaagaaa taagacacta tctacaaaca ggcgaacata
     5881 taccacaaat cccatttact gaacaactga gcatactcaa agaccaggta gagcgaagca
     5941 tcgaatggtt agacgaacgt agaggtattt tacatatccg taggcattta gctgccacac
     6001 ccttattcaa aggaatccca aactttaaag acacccgcat tgctatgttg cgtgccacaa
     6061 caaaagacga actttttgcc atttttaatg ctatagaaac tatggtggtg gagagataaa
     6121 taattaagga aaacacacat atttaatgat ttacagaatg attgtaagaa taacagctta
     6181 aatacgtcat aaccatatgg ttttttagta aactatttat atctttgaaa gttagaattt
     6241 tttatttaaa atgaacaaag aaaatcaaat agaagaaaat ttaattgagc agctaaaagg
     6301 gctgaaatac attcatcgcc ccgacatagt tgacagaaaa acgcttgaac aaaacttcaa
     6361 agcaaaattt gaagagctta accgtgttca cttatcagat aatgagtttt taagactcag
     6421 agaggaaatt ataaactctg atgtgttttt tgcatcaaaa aaattacgtg aaagacaata
     6481 ctttcaacgt gaggacggaa cacctttaca ttacacttta gtaaacatca aagattggtg
     6541 caaaaatgac tttgaagtta tcagtcaatt acgcatcaat actgagaata gtcaccaacg
     6601 atacgacatc attttgctga ttaacggctt gcctgtggtg caaatcgaac tgaaaagagg
     6661 agatatttcg catcgaaaag caatgcagca aattgtggat tacaaaaacg aaccgggcaa
     6721 cggttatgga aattcattgc tttgctttat gcaattattt attgtaagta atagtgggag
     6781 aacgctttat ttcgctaata ataagaatca gcattttgct ttcaatgcag acgagcagtt
     6841 tttgccagtt tatgaattag cggacataga caacaaaaaa attaacggac tttttgactt
     6901 tactgataag tttctaagca aatgtacgct tggtgaaatg attagtaagt atatggtttt
     6961 ggtagaaagt gaacagaaat tgctcgttat gcgaccttac caaatttatg cagtaaaagc
     7021 gattgaagaa tgtgttaagc aaaatagagg taacggatat atttggcata caacgggaag
     7081 cggtaaaacc ttaacgtctt tcaaggcttc tacgctttta aaggacaatc acgaaattga
     7141 aaaatgtttg tttgtggttg accgtaaaga ccttgaccga caaacccgag aggaatttaa
     7201 taaatttcag gaaggaagcg tggaagaaaa caccgataca gagacattag taaagcgttt
     7261 gctttctatg gattatgccg acaaagtaat tgtaactact attcaaaaat tgggcttagc
     7321 attagatggt acttacaaga aaaactataa agaacgatta gagccattaa gcaaaaaacg
     7381 tgttgttttc atctttgacg agtgccaccg ttcacaattt ggtgagaacc acaaagcaat
     7441 taaagagttt ttccctaatg ctcaattatt tggttttacg ggtacaccta tttttgatga
     7501 aaacgccact tacagcataa gagaaggaga atttgcttca tacaaaacta cggaggacat
     7561 ttttcaacag aggctacact cttatactat tacccacgca atagatgata aaaacgtttt
     7621 gcgttttcat attgattatt tcaaaggaaa taaaaaccaa aaacccggag aagcaatttc
     7681 aagacaggca gtagtagacg ccattttgaa caaacacaat gctgcgacaa attcaagaaa
     7741 gttcaatgct gttttggcaa cggcttcaat caataatgcc attgaatact atgaactttt
     7801 caaagaatta caaaagaaaa aacaaaaaga aaatcccgaa tatgttccgt taaatattgc
     7861 ttgtgtgttt tcgccaccct ctcaactcat tgcaacacag ggagaccaac aaagtcagaa
     7921 aaatgccgca gacatcaaac aattgcagga agatttgaca caagaacgtg aggataacaa
     7981 acaaaatcca gaagaaaaga aaaaagcact gacagaaatt attgaagatt acaacaagca
     8041 attcaatacc aatcatagca ttaacgaatt tgatttgtac tatcaggatg ttcaaagacg
     8101 aataaaagac caacaataca gcaacaaaga ttatccacac gaaaacaaaa ttgacattac
     8161 catagtggta gatatgttgc ttacaggttt tgacagcaaa tacttgaata ctttgtatgt
     8221 ggacaaaaat ctgaaatacc acggattgat tcaggcattt tcacgcacca atcgtgtatt
     8281 gaacgacacc aaaccttacg gtaacatttt agattttcgt tcgcaacaag aaagcgtaaa
     8341 tcaggcgatt gcacttttct caggagaaga taacggcaag gcaagagaaa tttggttggt
     8401 tgatcctgcc cctgtaatga ttgacaaata caaagaagcc gttgagaaat taggggtgtt
     8461 tatgcaggag cataatttgg tgaacgagcc acaagaagtt tataatttga aaggtgatgc
     8521 tgcaagaatt gcttttgtac aaaactttaa ggaagtgcaa cgcctcaaaa cacagcttga
     8581 ccaatacaca gacttagacg aacaacaaaa gg
//
LOCUS       DAOSRP010000085         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7805_length_8612_cov_5.485801, whole genome shotgun sequence.
ACCESSION   DAOSRP010000085 DAOSRP010000000
VERSION     DAOSRP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7805_length_8612_cov_5.485801"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLJ82_06235"
     CDS             <1..614
                     /locus_tag="PLJ82_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR41140.1"
                     /translation="LGITIIISVFIGSVITLLTVLDTADPLLPTYITGLVTRDTLLLE
                     FSSTILCLILAGKVGSNIASEIGTMRITEQIDALKIMGVNAPNYLIFPKIIAFCLIVP
                     VLVIFSMFTGIVGGYIITYTGVISPNDYILGIQYAFIPYYIVYSIIKSVIFAFIITSV
                     SAYYGYFSYGGALDVGKSSTNAVVNSSIIILLFNVLLTDLFLM"
     gene            617..1387
                     /locus_tag="PLJ82_06240"
     CDS             617..1387
                     /locus_tag="PLJ82_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR41141.1"
                     /translation="MIEVKNVCKSFDGVEVLKNVSALFHAGKTNLIIGQSGSGKTVLM
                     KSIIGIHHVTSGQILYNNRDFTAMSGDEVKALRQEVGMLFQGSALFDSMTVEENVRFP
                     LEMFSNLPKEAWDDRVNFCLNRVNLHNANKLFPSEISGGMQKRVAIARAIVLQPTYLF
                     CDEPNSGLDPKTAIVIDQLIQEITYEYNITTIINTHDMNSVMEIGDHVLFINKGEKSW
                     EGNKDSIFESDNEYLNDLVFASDLFKKIKKNHLLNKRT"
     gene            1384..2145
                     /locus_tag="PLJ82_06245"
     CDS             1384..2145
                     /locus_tag="PLJ82_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOR41142.1"
                     /translation="MNIISYNVNGIRSAISKGLIDWIKEENPDVLCFQETKAQPEQID
                     TSLFEALGYHCYFHSAEKKGYSGVAILSKTKPDNVVIGMGNPKYDSEGRVIRADFGDI
                     SVMSVYVPSGTTGGIRQDFKMEFLYDFTTFIHNLRLSYPKLVISGDYNIAHKPIDINH
                     PERQVGVSGFLPEERDWLTDFLESGFTDSFRAIDPSPEKYSWWSYRAGARTRNMGWRI
                     DYHLISQALVAQLKHAAILSDVVHSDHCPVSIDLT"
     gene            2164..2436
                     /locus_tag="PLJ82_06250"
     CDS             2164..2436
                     /locus_tag="PLJ82_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41143.1"
                     /translation="MPSFFHRPKPRQFHLTPRYYDEHKERIAEARKRVAQNSENKEEL
                     ADKPYYSAIKGKFGNVSNNLKSKNRQQIGMGIAIGILVLVLYFFFW"
     gene            2468..4300
                     /gene="mutL"
                     /locus_tag="PLJ82_06255"
     CDS             2468..4300
                     /gene="mutL"
                     /locus_tag="PLJ82_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOR41144.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPSSVIKELVENAIDAGATSI
                     QIIIKDAGRTLIQVIDNGKGMSETDARMSFERHATSKITNAADLFRLQTMGFRGEALA
                     SIAAIAHVELRTRRANDELGTCIHIKASVVEKQEEIACAVGSNFMIKNLFFNVPARRK
                     FLKTNETEFRHILTIFQQIALVYPAISFSLIHNETEVYQLASSNHRVRIGAIFGTKIN
                     QQLLPIEVNTQLISFFGFIGNPSFAQKRSANQYFFVNGRYIQHPYFYKAIALAYEKLL
                     PPDTKPSFFMYLQVDPSAIDVNIHPTKTEVKFEHEQAIFPILSAAVKETLGKTNAVPS
                     IDFDQDNALEIPVVSQDTYVAPPTIHVNPSYNPFKSTNFSAPTAPKTIGWESLYKNFD
                     AKHTPQSDNAFEHQFEEVIDNEQLSLYPSHEGNKAMLQLRKHYIVLPTENGITIIHQH
                     RAHMHLLYTQYVENMQQKRGISQQLLFPEIVELTPQEHSCLLEILDDISYIGFRLEEF
                     GKHAYQLAGVPTQLESATCADVLHALLESVQETGNTLKTDMQQTIALTLAKKAAIKTG
                     ATLSHEEMNEIVQSIHQGTIPAYTTDGKKILSTISFAEIDKTFN"
     gene            4325..5839
                     /gene="gltX"
                     /locus_tag="PLJ82_06260"
     CDS             4325..5839
                     /gene="gltX"
                     /locus_tag="PLJ82_06260"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR41145.1"
                     /translation="MKQVRVRFAPSPTGPLHIGGVRTALYNYLFAKKHNGKFILRIED
                     TDTNRFVPGAEAYIIESFNWLGIQFDEGVSFGGNYGPYRQSERKEIYKVYVKELLDKQ
                     LAYIAFDTPEELDTKRAEISNFQYDASTRMQMCNALTLSANEVAQRIANGDNYVVRIK
                     IEPNQDIIVQDIIRGEVKINSSVLDDKVLYKSADELPTYHLANIVDDHLMEITHVIRG
                     EEWLPSAPLHVLLYRYFGWEDTMPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLQW
                     TDPKTGDTSAGYRENGYFPEAVVNFLALLGWNNGTEQELFSLDDLVHEFSLDRVSKSG
                     AKFDYEKGKWFNHTYLIHKTDTELASLFMPIVQAKGYAITEAKLTHIVGLLKERAHFV
                     ADLWEQAAFFFEAPTQYDDKAVQKRWKETTASMLAELAVFIQSLPNIDDSTHTEETVK
                     QWVETKGYGLGNVMNPLRLALVGELKGPHIFDITAILGKEETIKRIQKAIDTLG"
     gene            5839..7062
                     /locus_tag="PLJ82_06265"
     CDS             5839..7062
                     /locus_tag="PLJ82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOR41146.1"
                     /translation="MGAIYSLVIRIYGFLIYIAGFFSEKAKLLYKGQRNTLWLAENSI
                     DKNQPYIWVHAASLGEFEQGRPLIEAIKKDHPTYKIVLSFFSPSGYEVRKDYALADYV
                     CYLPLDTKANATRFIELIQPVKAIFIKYEFWPNYLQVLRKKGIETYIVSAIFRKEQIF
                     FSWYGGWYKKLLQCFTHIFVQDTSSFELLQTHGIHRVSIAGDTRFDRVATIAASPTQL
                     PIIESFKQDKKLIVLGSSWEPDEALLIPFVEKNDCKLIIAPHQIHTERIQSLLAKLSM
                     PTALYTQTTAEDVKHANCLVINTIGILSSVYRYADVAYIGGGFGVGIHNILEAAVFGV
                     PVLFGPNYHKFKEAVDLINEGGAFCIQTQTDIDSIGKNLLTDNAFYQQVCTQSASFVQ
                     HRIGATQGIMNQLFV"
     gene            7066..8577
                     /locus_tag="PLJ82_06270"
     CDS             7066..8577
                     /locus_tag="PLJ82_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOR41147.1"
                     /translation="MKVKLVLLSMMLFSLLVVTQAQIAPKREFRGVWMHTVFSDDYAQ
                     KSNEQIKNFLLQRLNEYQSVGMNVLIFQVRPEADAFYKSSYEPWSRFLTGKQGKAPAD
                     SFDPMTFLIDECHKRGMEFHAWLNPYRANANRKNELHPDHLYWKRKDLFVYFGNQLFF
                     NPGIPENRTHIAKVVKDIVARYDVDAIHMDDYFYPYPIKGSSFPDQKEFVKYGLQTGE
                     FKLYQKDDWRRNNVNLLIKELNTVIKKEKPWVKFGISPFGIYRNQRQYAEGSETNGLS
                     NYDDLYADVKLWVKNGWIDYNIPQLYWEIGHKQADYETLIDWWSKHNFDGHLYIGQDI
                     DRTMKAADLQHKNKNQLSKKMELVRNNPKISGNCFWSADMLVRSHALKDSLRNNYHRY
                     KALVPVVKSLDSIPPAPVTDLRFAMMGKEPKLVWNVKKTNKELDKPTQFVVYAFERND
                     KIDINNPRYILDIVNKPEYVLPKTPNALCLYYVVTALDRVKNESLPSAPAMYK"
BASE COUNT         2835 a         1728 c         1638 g         2411 t
ORIGIN      
        1 cgttaggcat caccattatc atatcggtgt ttattggttc ggttattacc ttactaacag
       61 tattagatac agctgaccca ttgctaccta cttacataac tggtttagta actcgagaca
      121 ccttattatt ggaattttca tctactattt tatgcctcat tttagcggga aaggttggtt
      181 ctaatattgc ttcggaaatt ggcaccatgc gtattaccga acaaattgat gccttaaaaa
      241 ttatgggggt gaatgctcct aactacctta tttttccaaa aataattgct ttttgtttaa
      301 ttgtgcctgt attggttatt tttagtatgt ttacaggtat tgtgggtggc tatattatca
      361 cgtataccgg tgttatttct ccaaacgatt atatcttggg tattcaatac gcgtttatac
      421 cttactacat tgtatattca atcattaaat cggtaatttt tgcctttatc attacatcgg
      481 tgtcggcgta ttacggatat ttctcatacg gaggagctct tgatgtgggc aaatcgagca
      541 ccaatgcagt agttaacagc agcattatta tcttgctatt caatgtatta ttaaccgatt
      601 tattcttgat gtagccatga ttgaagtaaa aaacgtatgc aaatcatttg acggtgttga
      661 ggtattaaaa aatgtttcgg cactatttca tgctggcaaa acaaacttaa ttatcgggca
      721 aagcggttcg gggaaaacgg tactaatgaa aagtattatt ggtattcatc acgttactag
      781 cggtcaaatt ttatacaata accgcgattt tacagctatg agtggcgacg aagtaaaggc
      841 attgcgtcaa gaagtaggca tgctgtttca aggttcggct ctgttcgact ccatgacggt
      901 agaagaaaat gtgcgttttc cgttggaaat gttttctaac ctgcccaaag aagcttggga
      961 cgaccgcgta aatttctgcc ttaatcgtgt taacttgcac aatgcgaaca aattatttcc
     1021 ttctgaaata agtggcggta tgcaaaagcg tgtagccatt gcaagagcaa tagtattaca
     1081 accaacctac ttgttttgcg acgaacccaa ttctgggctt gaccccaaaa cagcgattgt
     1141 aatcgaccaa cttattcaag aaataaccta cgaatacaat ataaccacca ttatcaacac
     1201 ccacgatatg aattcggtta tggaaattgg tgaccatgta ttgtttatta ataaaggaga
     1261 aaaaagctgg gaaggaaaca aggattcaat tttcgaatcg gataacgagt acctcaatga
     1321 cttggtattt gcttctgatt tgtttaaaaa aatcaaaaaa aaccatttgt taaataaacg
     1381 cacatgaaca taatatcgta taacgttaat ggcatacgct ctgctataag caaggggtta
     1441 attgattgga taaaagaaga aaatcctgat gtattatgct ttcaagaaac caaagctcaa
     1501 cctgagcaga ttgatacaag tctatttgaa gcattaggat atcattgtta ttttcattca
     1561 gctgaaaaaa aagggtacag tggtgtagct attttgagca aaacaaaacc cgacaatgta
     1621 gttattggca tgggcaatcc caaatacgat agcgaaggtc gcgttatcag agctgatttt
     1681 ggtgatataa gtgtgatgag tgtatacgtg ccatcgggta ctactggggg cattcggcaa
     1741 gattttaaaa tggaattttt atacgacttt actaccttta ttcataattt acgcctctcg
     1801 tatcctaaac tagtaattag tggcgattat aacattgcac acaagcctat tgatatcaat
     1861 caccctgaaa ggcaagtagg tgtttcggga tttttgcccg aagaacgcga ctggctcact
     1921 gactttttag aaagcggttt taccgactca tttagggcta ttgacccttc acctgaaaaa
     1981 tacagttggt ggagttatcg agcaggagcc agaaccagaa atatgggttg gcgtatcgac
     2041 tatcatctta tcagccaagc cttagttgca caactaaaac acgctgccat tttaagtgat
     2101 gtcgttcatt ctgaccattg cccagtaagc attgatttaa cgtaaaatta aacgaaactg
     2161 attatgccaa gtttttttca tcgtccaaaa ccacgtcaat ttcatctaac ccctcggtat
     2221 tatgatgaac acaaagagcg tattgctgaa gcacgcaaac gtgtagcaca aaacagtgaa
     2281 aataaggaag agctagcaga caaaccttac tattcggcta taaaaggtaa atttggcaat
     2341 gtaagtaata acctcaaaag caaaaaccgt caacaaatcg gtatgggtat agctattggc
     2401 attctggtat tggttcttta ttttttcttt tggtaatggt tcttttgtag aataagtaca
     2461 caacactatg tcagatataa tccatttatt acccgactcg gtagctaacc aaatagcagc
     2521 aggtgaagtt atacaacgcc cctcgtctgt tatcaaagag ttagtagaaa atgctattga
     2581 tgcaggtgct acttctattc aaatcattat taaagatgct ggcagaaccc taatacaggt
     2641 tattgataat ggaaaaggga tgtcagaaac agatgctcgc atgagttttg agcgtcatgc
     2701 tacatccaaa atcaccaacg ctgctgattt atttcgctta caaacaatgg gctttagggg
     2761 cgaagccttg gcttctattg cagccatcgc tcacgtggag ttgcgtactc gacgtgccaa
     2821 cgacgaactg ggtacgtgta ttcatatcaa agcatcggta gtagaaaaac aagaagaaat
     2881 agcgtgtgcc gtaggcagta attttatgat taagaaccta ttttttaatg tacctgccag
     2941 acgcaaattt ttaaaaacga acgaaacaga atttcggcat atactcacta tttttcagca
     3001 aatagcgttg gtatatccag ctatttcttt ttctcttatt cataacgaaa cggaagtata
     3061 tcaactagct agtagcaacc accgcgtacg tattggagct atttttggca ctaaaatcaa
     3121 ccaacaattg cttccgatag aagtaaacac acagcttatc tcattttttg ggtttatagg
     3181 taatccttct tttgctcaaa aacgatcggc aaatcagtat ttttttgtaa atggtaggta
     3241 cattcaacat ccctattttt acaaagccat agcattggca tacgaaaaac tactaccacc
     3301 agataccaaa ccttcttttt tcatgtattt gcaagttgac ccatcggcaa ttgatgtaaa
     3361 cattcatcca acgaaaacag aagtgaaatt tgagcatgaa caagccattt ttccaattct
     3421 atcagcagcg gtaaaagaaa ccttaggcaa aacaaatgca gtgccctcta ttgattttga
     3481 ccaagataat gcactcgaaa tacccgttgt aagccaagat acatatgtgg ctccaccaac
     3541 gatacacgtt aacccatctt acaacccatt taaatcaacg aatttttcag cacccacagc
     3601 tcctaaaaca attggctggg aatcgttgta caaaaatttt gatgctaaac atactcctca
     3661 gtctgataat gcgtttgagc atcaatttga ggaagtaatt gacaatgaac aactttcatt
     3721 atacccttcg cacgaaggca acaaagctat gttgcaactt cgcaaacact atattgtatt
     3781 gccaactgaa aatggcatta ccatcattca ccagcataga gctcacatgc acttattata
     3841 tactcagtat gtggaaaata tgcaacaaaa acgaggtatt tcgcaacaac tattgtttcc
     3901 ggaaatagta gaactcacac ctcaagagca tagttgcttg ctcgaaatac ttgatgacat
     3961 atcgtatatt gggttccgtc ttgaggagtt tggcaaacat gcctaccaac ttgcaggggt
     4021 tcctactcaa ttagagagtg ctacttgtgc agatgtgttg catgccttac tcgaatctgt
     4081 tcaagagaca ggaaatactc ttaaaacaga tatgcaacaa acaatagccc ttaccttagc
     4141 gaaaaaagct gctattaaaa caggtgcaac attgtcgcac gaagaaatga atgaaatagt
     4201 acaatccatt catcaaggaa caatacccgc atatacaact gatggtaaaa aaatactatc
     4261 aacaatttca tttgccgaaa tagataaaac gtttaattaa acaacacatt tagatacaag
     4321 attcatgaaa caagttagag ttcgttttgc tccctctccc acaggtcctt tgcacatagg
     4381 cggtgtacgc acagccttgt ataattattt atttgcaaaa aaacacaacg gaaaatttat
     4441 cctacgtatc gaagataccg ataccaaccg ctttgtgcca ggtgctgaag cgtatattat
     4501 cgaatctttc aactggctcg gcattcaatt tgacgaaggt gtatcgttcg gaggcaacta
     4561 cggtccgtat cgccaaagcg aacgcaaaga aatttataaa gtctatgtaa aagaactatt
     4621 ggataaacag ttggcatata ttgcttttga tacgcccgaa gaactcgaca caaaaagagc
     4681 agaaatttcc aactttcaat acgacgctag cacacgtatg cagatgtgca acgcacttac
     4741 cttatcggca aatgaagtgg ctcaacgcat agccaatggc gataattatg ttgttcgtat
     4801 caaaattgaa ccaaaccaag atattattgt acaagacatt attagaggcg aagtaaaaat
     4861 taactcatcg gtattagacg ataaagtgct atacaaatcg gcagacgaac tgcccaccta
     4921 ccatttagcc aatattgtag atgaccattt aatggaaata acgcacgtga ttcgcggcga
     4981 agaatggttg ccttcagctc ctttgcacgt attgctctac cgctattttg gttgggaaga
     5041 caccatgcct caatttgcac atttacccct attgctcaaa cctgatggca atggtaaact
     5101 cagcaaacgc gatggcgacc gattgggctt tccggtattc cccttgcaat ggacagaccc
     5161 caaaacggga gatacatcgg ctggataccg cgaaaatggc tattttccag aagcagttgt
     5221 aaactttttg gcactattag gatggaataa tggcacagaa caagaactat ttagtttaga
     5281 tgatttagta cacgaatttt cgttggaccg tgtgagtaaa agtggtgcta aatttgatta
     5341 cgaaaaaggg aaatggttta atcataccta ccttatccac aaaacagata ctgaattagc
     5401 aagtttattt atgcctattg tacaagctaa aggatatgca ataactgagg ctaagctaac
     5461 acatatcgtt ggtttgctaa aagaacgagc acattttgtg gctgacttat gggaacaagc
     5521 ggctttcttt ttcgaagctc ctacacagta cgatgacaaa gcagtgcaaa aacgttggaa
     5581 agaaaccact gccagtatgt tggctgaatt agctgttttt attcaatcct tacccaacat
     5641 tgacgatagt acccatacag aagaaactgt aaaacaatgg gttgaaacaa aaggatatgg
     5701 acttggcaat gtaatgaacc ccttgcgttt agccttggtt ggtgaactaa aaggtcctca
     5761 tatatttgat attaccgcca tactaggcaa agaagagaca ataaaacgaa tacaaaaagc
     5821 gatagataca ttaggctaat gggagcaata tattcactag taatccgcat atatggattt
     5881 ctcatttata tagctgggtt tttttcggaa aaagcaaaac tgctctacaa ggggcagcga
     5941 aacacccttt ggttagctga aaatagtata gacaaaaatc agccctatat ctgggtgcat
     6001 gcagcttcgt tgggcgaatt tgaacaagga agacccctta tcgaagccat aaaaaaagac
     6061 catccaacat acaaaatcgt acttagtttt ttttccccct ctggctacga agtaagaaaa
     6121 gattatgcac tagcagatta tgtatgctac ctaccactcg ataccaaagc caatgctaca
     6181 cgctttattg agctaataca accagtaaaa gcgattttta tcaaatacga attttggccc
     6241 aattacttgc aggtattacg caaaaaaggc atcgaaacat acattgtttc ggcaatattt
     6301 cgcaaagaac aaatcttttt ttcgtggtat ggtggctggt acaaaaaact attacagtgt
     6361 tttacccata tctttgtaca agataccagt tcttttgagc tacttcaaac tcatggcata
     6421 caccgagtaa gtattgccgg cgacacccgt tttgacaggg tagctactat tgcagcttca
     6481 cctacgcaac tacctattat tgaatctttt aaacaagata aaaaacttat cgttttgggt
     6541 agttcgtggg agccagacga agccctactt ataccgtttg tggagaaaaa cgattgcaaa
     6601 ctgataattg caccgcatca aattcacacc gaacgcatac aaagtttact tgcaaaacta
     6661 tctatgccaa cggctctcta tacccaaacc acagcagagg atgtaaaaca tgccaattgt
     6721 ttagtaatca ataccattgg tatattgtca tcggtatatc gctatgcaga tgtagcgtat
     6781 attggtggag ggtttggcgt aggtattcac aatatcttag aggcggctgt atttggtgtt
     6841 cctgtactct ttggtccgaa ttaccacaag tttaaagaag ctgtcgactt gataaacgaa
     6901 ggtggagctt tctgtataca aacgcaaaca gatatagatt caattggcaa gaatctactc
     6961 acagacaatg ccttttacca acaagtatgt acccaatccg cttcgtttgt acagcacaga
     7021 ataggagcaa cacaaggtat tatgaaccaa ctatttgtat aaaatatgaa agtaaaacta
     7081 gtactacttt caatgatgct cttttctcta ttggtggtaa ctcaagctca aattgcccct
     7141 aaacgcgaat ttagaggcgt atggatgcat acggtattta gcgacgatta tgctcaaaaa
     7201 agcaacgaac agataaaaaa tttcttactt caacgcctca acgaatacca atcggtaggc
     7261 atgaatgtac tgatatttca agtacgcccc gaagccgatg ctttttacaa atcgtcttac
     7321 gaaccatgga gccgttttct tacgggcaaa caaggaaaag ctcctgccga tagtttcgac
     7381 ccaatgactt ttttaattga cgaatgccac aaaagaggga tggagtttca tgcatggctc
     7441 aacccatatc gtgccaatgc aaaccgcaaa aacgaactgc accccgatca cttatattgg
     7501 aaacgaaaag atttatttgt gtattttggc aaccaattgt tttttaatcc cggtataccc
     7561 gaaaaccgca cccatatagc caaagtagta aaagacattg tagctcgcta tgatgtagat
     7621 gcgatacaca tggacgatta tttttatcct tatccgatta aaggaagttc gtttcccgac
     7681 caaaaagaat ttgttaaata cggcttacaa acaggagaat ttaaactgta ccaaaaagac
     7741 gactggcgta gaaataatgt caatttactg attaaagaac tgaatacggt tatcaaaaaa
     7801 gaaaaacctt gggtaaaatt tggcattagt ccttttggta tctaccgcaa ccaacgtcaa
     7861 tatgccgaag gtagcgaaac caacggtcta tctaactacg acgacctata cgctgacgta
     7921 aaactatggg tgaaaaatgg ctggattgac tacaatatac ctcaactata ttgggaaata
     7981 gggcacaaac aagccgacta cgaaacgcta attgactggt ggagtaaaca caattttgat
     8041 ggacacctat acattggaca agacattgac cgcacaatga aagctgccga cttgcaacat
     8101 aaaaacaaaa accaattaag taaaaagatg gaattggtac gcaacaaccc aaaaattagc
     8161 ggtaactgct tttggagtgc cgatatgctg gtgcgtagcc acgccctaaa agatagctta
     8221 cgcaacaact accaccgcta caaagcccta gtgcccgtag taaaaagtct cgatagcata
     8281 ccacccgctc ctgttaccga tctacgtttt gccatgatgg gcaaagagcc caagctggta
     8341 tggaacgtaa aaaagactaa caaggaacta gacaaaccta ctcaatttgt ggtgtatgct
     8401 tttgaacgaa acgacaaaat tgacatcaac aacccacggt acatcctcga tattgtaaac
     8461 aagcccgaat acgtgttgcc caaaactcct aacgccctct gcctatatta cgtagtaacc
     8521 gccctcgata gggtaaaaaa cgaaagcctg ccttcagcac cagcaatgta taaatgagtg
     8581 gaaaaatata gtgatagaaa accttgtgcc ac
//
LOCUS       DAOSRP010000086         8583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_7837_length_8583_cov_5.426243, whole genome shotgun sequence.
ACCESSION   DAOSRP010000086 DAOSRP010000000
VERSION     DAOSRP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7837_length_8583_cov_5.426243"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..259)
                     /locus_tag="PLJ82_06275"
     CDS             complement(134..259)
                     /locus_tag="PLJ82_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41148.1"
                     /translation="MAHLVFPDTRANAQKTKRAIFLPTHGQTTEHKKLNLQTENF"
     gene            complement(435..1109)
                     /locus_tag="PLJ82_06280"
     CDS             complement(435..1109)
                     /locus_tag="PLJ82_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOR41149.1"
                     /translation="MGYYIDLEKISIDDYRIKLESAYLPPSRLILKDKLDERFGYFKS
                     IGIKNVKGLIQILKKKDKLVELSKVDFLSGDYLTILLRELNSTLPKPNKISDFIGIAK
                     GTIDSLDKIGISNTEKLYDKVVKQSDRQKLADSTGIKYQDILELTKLSDLSRVKWVGV
                     TYAQMLYDLGVDSVEKVSVSDPVDLHARINQMIKEKNIFKGSIGLNDVKILIESASEL
                     PLEIEY"
     gene            complement(1808..1895)
                     /locus_tag="PLJ82_06285"
     tRNA            complement(1808..1895)
                     /locus_tag="PLJ82_06285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1859..1861),aa:Ser,seq:gga)
     gene            complement(1902..2684)
                     /locus_tag="PLJ82_06290"
     CDS             complement(1902..2684)
                     /locus_tag="PLJ82_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR41150.1"
                     /translation="MHLIDTHSHIYLSDFDADREEVVLRAQSVGVKHVILPNIDASTI
                     QPLHVTANTYAGLCHPLIGIHPTSVKADYKDEIAFFKAQFEQYPYIGIGEIGIDLYWD
                     KTYLHEQIEVFEYQVDMAIKHALPVIIHCRESFRETMQSLQHFDAKQLKGIFHSFIGT
                     PDEANEIFGAGNFKLGINGVVSYKNAIFAQQLQHIPLSAIVIETDAPYLTPVPHRGKR
                     NEPSYLPHVVKKLAEIYGVSEEEIAQTTSNNAYQVFPSLIDV"
     gene            complement(2689..3384)
                     /locus_tag="PLJ82_06295"
     CDS             complement(2689..3384)
                     /locus_tag="PLJ82_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOR41151.1"
                     /translation="MKYKHLFFDLDNTLWDFDKNSQVSLHELFDAYQLIHYYPRFEDF
                     FEIFKKQNEVLWEMYGKGQIDKNFLHYERFRFPFENTPFEAQLRYKELADEYLLVVKT
                     KTHLKPFAKEILEYLKDKNYMMYIVSNGFTEVQYHKLENAGIGTYFQRIFLSESIKEH
                     KPSKAFFDYAITSSNARKTESLVIGDNWEADIVGAKNAGIDQVFYNYTNRIDLPFAPT
                     FQVSCLSDLKNLI"
     gene            complement(3463..4314)
                     /locus_tag="PLJ82_06300"
     CDS             complement(3463..4314)
                     /locus_tag="PLJ82_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41152.1"
                     /translation="MKKLLLASAILAILFSSCGYESKEFKALKAKNDSILAVQEQQIA
                     EMNEYMALVQEIDNGFSAIKQSQDMLSISSSYEGQPNESLRTRFTNDLNTINQILADN
                     KSKIADLEKKVESGKFQSSELRRTVNRLTETLNQKNQELEALSAVLAKKDITIDSLVV
                     ETQALLRKTADLEMEKNVNLQEIAKQDADLNRVYYKLATRKEIKDSNIDVDKMKSTVR
                     NGMFTAADKRELDAVEINSKRAKVLTKHPVSSYELRQKADKTYTLIIKNHTDFWSMSK
                     YLLVRID"
     gene            complement(4417..5130)
                     /gene="rsmI"
                     /locus_tag="PLJ82_06305"
     CDS             complement(4417..5130)
                     /gene="rsmI"
                     /locus_tag="PLJ82_06305"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOR41153.1"
                     /translation="MKLFVVPTPVGNLDDMTFRAIEVLKKVDFILAEDTRTSAVLLKH
                     FDIKNTLIAHHKFNEHKGLQPIIERLLAGQTGAVISDAGTPGISDPGFLIVRACIEHN
                     IPVECLPGATAFVPALVNAGLPMDRFCFEGFLPQKKGRQTRLQQLKFETRTIILYESP
                     YRVVKTLTQLAEILGQNRKASVSREISKLYNETINGTLADIIAHFVQKQPKGEFVLVI
                     AGANVKHAELDDSSSEEDE"
     gene            complement(5140..5868)
                     /locus_tag="PLJ82_06310"
     CDS             complement(5140..5868)
                     /locus_tag="PLJ82_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41154.1"
                     /translation="MEKNILIDQMLKDIKELEFIVNGVRTMNTIHPIMQQLVTRKFKN
                     LEENFSLVLQDSVPTDKGAPKFTDVNDEEDSITSTDDTTTTILLDEDIEEIEFVDTRK
                     KEQPVSQPTVKSSTSPTIPPSQNEPRTYTQQKSSIQPETTIHKETPKPASVILATPKT
                     SAVDARLVTDLRRAIGINDRFRFKRELFGGDEQKMMETIDILNQCSSLQEVEEYIDAH
                     FDWQKEDSTYVYFSEILHKRFAHI"
     gene            5933..6523
                     /locus_tag="PLJ82_06315"
     CDS             5933..6523
                     /locus_tag="PLJ82_06315"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOR41155.1"
                     /translation="MIYLETSRQSEKRRGSIEVICGSMFSGKTEELIRRLKRAKIAQL
                     HVEIFKPAIDVRYSETEVVSHDKNSILSTVVDSSQNILLLSNDVDVIGIDEAQFFDMG
                     LIDVCRQLANQGMRVIIAGLDMDFKGNPFGPMPALCAIAEHVTKVRAICVKCGHLALY
                     SYRLTNSDKQVLLGETADYQPLCRECYHESTTKLKE"
     gene            6619..7989
                     /locus_tag="PLJ82_06320"
     CDS             6619..7989
                     /locus_tag="PLJ82_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR41156.1"
                     /translation="MKQLDNANERFAQLLKNYESNLTGGYFDAEEMEQLADFYLRRMR
                     VKDAKRAIEFGLKLHPNNIGLETSKVRILIELNDLELALQHTNSLVALYPSDIELHLL
                     QTEILLKTKQEVQAEKNCKELINTYAQEDETIYLDIAYVYLNNEYYQKALAFLLIGHT
                     RNVYNINILFEIAFCYEKLNQIDNSIATYHRILDIEPYSDEAWFNLGQVFYSQKDYAK
                     AIEAYEYATAINEEDAMAWQLLAHAYYQNDNYKKAAETYLMYAEQTEHKATPLLFAAE
                     SYEKMEDFDLALHYYSMVLDMQPNNTEAMVGVGICHLEKEQFQKSINYFVRAIELDPT
                     LSEAWVYLGEAYINLDDPSKGLQAYLTSIELNSNQPETLTSIGNLYLEQENFSMALHY
                     YLTAFELDHETDNIHLFLAMAYYGEENYLPAKEHLQKAINVNTEALNLFLEIYPDALQ
                     YINLTT"
     gene            8062..>8583
                     /gene="aroE"
                     /locus_tag="PLJ82_06325"
     CDS             8062..>8583
                     /gene="aroE"
                     /locus_tag="PLJ82_06325"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOR41157.1"
                     /translation="MKQYGVIGNPLHHSYSKAYFNEKFQEQAIDARYDNYLLPTIKDV
                     MKVLNDTPNLCGLNVTIPYKEQVMEYLDEIDDEAKEIGAVNVIKITEKNGKRFLKGYN
                     SDWFGFTEAIKPFLKPHHTHALILGTGGASKGILYALKKLKINTQFVSRDPSKGLTYK
                     QLTKEILQQYTIIV"
BASE COUNT         2727 a         1565 c         1382 g         2909 t
ORIGIN      
        1 cgtgtttcac caaatcaagc atagcgttta cttcacgttg gtttgtaaac acttccccgt
       61 ggtcggtaac ccgctttttg gattttactt ggttgtcttt ttttatttct tctgtcaatt
      121 cgttcacctt atattagaaa ttctcagttt gtaaatttag ttttttatgt tccgttgtct
      181 gtccgtgcgt tggcaaaaaa atggctcttt tggttttttg agcgttggct cgtgtgtcgg
      241 gaaaaaccaa atgtgccatt tgcgggtcgg ctttttacac tgaccgataa cggatgggta
      301 tatgaaacgt ttggcatttc aaagcgattt cctatcaaac cgttgcgatg tttattaaat
      361 gtactgccat taaaattagc actttccgcc aaatgcttta tataccgtgt tggcagctgg
      421 tgtttattca tctcttaata ttcaatttct aaaggtaatt cactcgctga ttctattaat
      481 attttcacgt cattcaatcc gatagatcct ttgaaaatat ttttctcttt tatcatttgg
      541 ttaatccttg catgtaaatc aaccgggtca gatacagata ctttctcaac tgaatcaact
      601 cctaaatcgt acagcatctg tgcataagtc actccaaccc atttaaccct tgataaatca
      661 gataatttgg ttaattctaa aatgtcttga tattttattc ctgttgaatc agcaagtttc
      721 tgtctatctg actgtttaac gaccttgtca tatagctttt ctgtatttga aattcctatt
      781 ttatccaaac tgtcaatcgt accttttgcg ataccgataa aatcagaaat cttattgggt
      841 tttggaagag tactatttaa ttcacgaagc agaatagtca agtaatcacc agatagaaag
      901 tcaacctttg atagctcaac tagtttgtcc tttttcttga gtatctgaat caatccttta
      961 acatttttaa tcccgatact cttaaaatac ccaaatcgct catccaattt gtctttaaga
     1021 atcaatctac tcggtggcaa atatgctgat tctaatttta tccgataatc atcaattgat
     1081 attttttcta agtcaatata gtatcccata ttttaatcct tttttagtga ttatcaatgt
     1141 gtcctgtggt tcttacactt gctgccaaca tctgtgtagg tacaccacaa gtgcacctat
     1201 ttcgacttta tattatagtt tttgtaattt aatatactga taataaaaca cttatgtttt
     1261 agtgtacatt tactcaaagg tacacctatc caatttgtta atttcatatt ttttatgctt
     1321 tgaattcgtt atatttctaa tttatccaac ggatttttta tttgattaaa tttccgtaaa
     1381 actactaaag tttttttatt taacaaaata gttgagtcta ttttacgcga tgcaatcata
     1441 ctactattgg gtaaaacaat cttttcctga gttacattct atattgtttt gtcatatatg
     1501 cgtttagcaa agggaggtat gaggtaagtt tgttacaaaa aaaatcaccc atctcaatct
     1561 ttatgggtat taaaatggga gataaattta agacgaattg atatcaagct actcctccct
     1621 acccctcgtt tacacaacga ggggtttatg gtttgtcgtt tgtaacgaca gcaatacact
     1681 acaccaatga tagctggtta attccttgat ttccaattag gtaataaaaa ctttaaaaag
     1741 gattacatac ccctatttac tgaaacgaca aaagtccaaa ctattttgga cttttgtcgt
     1801 atttttgcgg agagaacggg attcgaaccc gtgataccct tttggggtat acacgctttc
     1861 caggcgtgcc tcttcaacca ctcgagcacc tctccttttt tttacacgtc tatcaagctt
     1921 ggaaatactt gataagcatt attgcttgtt gtttgtgcaa tttcttcttc acttactcca
     1981 tatatttctg ctagcttttt tactacatgc ggcaaatatg atggctcgtt tctttttccc
     2041 ctatgtggca ctggtgttag atagggagca tctgtttcta taacaatagc cgacaatgga
     2101 atatgttgca attgctgtgc aaagatagca tttttataac ttactacacc attgataccc
     2161 aacttaaaat tacctgctcc aaaaatttca tttgcctcat caggggtacc tataaaacta
     2221 tgaaaaattc cttttagttg ttttgcatca aaatgctgca aactttgcat agtttctctg
     2281 aacgactccc tgcaatgtat gatcacgggc aatgcatgtt tgatagccat atctacttga
     2341 tattcaaaaa cttctatttg ttcgtgcaga tacgttttat cccaatacaa atcaatgcca
     2401 atttcaccta tcccgatata cggatactgc tcaaattgtg ctttaaaaaa cgcaatttca
     2461 tctttgtaat cagccttaac actggttgga tgaataccta ttaatggatg acacaacccc
     2521 gcatatgtat tggcggtgac atgcaatggc tgtatggtag atgcatcaat atttggcaaa
     2581 ataacatgtt ttacgccaac agattgtgcc cgcaaaacaa cctcctccct atcagcatca
     2641 aaatcggaaa gatatatatg agagtgtgta tcaatcagat gcataggatt aaataagatt
     2701 ttttaagtca gacaaacaac ttacctgaaa tgtaggggca aatggcaaat caatacggtt
     2761 cgtataattg taaaaaacct ggtctatgcc tgcattttta gctcctacaa tatctgcttc
     2821 ccagttatca ccaatcacca aactttcagt ctttcgagca tttgatgatg taattgcata
     2881 atcaaaaaat gctttcgatg gtttatgttc tttaatggat tcggataaaa atatacgttg
     2941 aaaataggta ccaatacctg cattttccag tttatggtat tgcacctctg taaatccgtt
     3001 ggatacaatg tacatcatgt aatttttatc cttgaggtat tccaatattt ctttggcaaa
     3061 aggtttaaga tgcgtttttg ttttcactac caacaaatac tcatcggcta actctttata
     3121 acgcaattgt gcctcgaagg gtgtattttc aaaaggaaaa cgaaacctct cgtagtgcaa
     3181 aaaattttta tctatctgcc ctttcccata catttcccat aacacttcgt tttgtttctt
     3241 aaatatctca aaaaaatcct caaaccttgg gtagtaatgt attagctggt atgcatcgaa
     3301 aagctcgtgc aagcttactt gcgaattttt atcaaaatcc cacaaggtat tatctaaatc
     3361 aaaaaataga tgtttatatt tcatttctta ccacgatata aaaacaaaag tacgcagaaa
     3421 ataaattctt gcgtactttt accaccttta tttaactttt tactaatcaa tacgtaccaa
     3481 taaatattta gacatactcc aaaaatcagt atggttttta ataatcaagg tatatgtttt
     3541 atcagctttt tgacgtaact cgtatgaact aactgggtgc ttagttaaca ctttagcacg
     3601 tttcgaatta atttcaacag catccaattc acgtttatcg gcagcggtaa acattccatt
     3661 gcggacagtt gatttcattt tatctacatc aatattgcta tctttaattt ctttgcgtgt
     3721 cgccagttta tagtatacac gatttaaatc agcatcttgt ttggcaattt cttgcaaatt
     3781 tacatttttt tccatttcca agtcagctgt tttgcgtaac aatgcttgtg tttctaccac
     3841 taaactatca atcgttatgt cttttttagc caacacagca cttaatgctt ctagttcttg
     3901 atttttttgg tttaatgttt cggttagtct atttacggta cgacgcaatt ctgacgattg
     3961 aaactttccg ctttctactt ttttctctaa gtcggcaatt tttgacttgt tatcggctaa
     4021 aatttggtta atcgtgttca aatcatttgt aaaacgtgta cgcaatgatt cgttaggttg
     4081 accttcgtaa gaagaactaa tactcaacat atcttgcgat tgtttgatag ccgaaaagcc
     4141 attatcaatt tcttgaacaa gagccatata ttcattcatt tcggcaattt gttgttcttg
     4201 tactgccaaa atagaatcat tctttgcttt aagagccttg aattctttgc tttcataccc
     4261 acatgatgaa aacagaatag caagaattgc agatgctaat agtaattttt tcataaaata
     4321 atgttttaaa aaagtgttac ttgtgttttt cggttacaaa gatacataat caattacaaa
     4381 tcaagtattt ttttagataa aaatttggat aaaatttcat tcatcctctt ctgatgaaga
     4441 gtcatccaat tccgcatgtt ttacatttgc acctgcaatt actagcacaa attcaccttt
     4501 gggttgtttt tgcacaaaat gagctatgat atccgccaag gtgccattaa ttgtttcgtt
     4561 gtacagtttt gatatttcgc gcgatacaga ggctttacga ttttgtccca atatttctgc
     4621 caactgcgtt aatgttttca ctacccgata cggagattcg tacaaaataa tggtacgcgt
     4681 ttcaaatttt aattgttgca aacgtgtttg tctgcctttt ttttggggta aaaagccttc
     4741 aaaacaaaaa cggtccatgg gcaaaccagc attaactaat gcaggaacaa aagctgttgc
     4801 tcctggcaaa cattctactg gaatattgtg ttcgatacag gcacgtacta tcaaaaaacc
     4861 aggatcagaa ataccaggtg tcccagcatc agaaataaca gcccctgttt gtcctgctaa
     4921 caagcgttct attatgggtt gtaatccttt gtgctcgtta aatttatgat gagcaataag
     4981 ggtgttttta atatcaaaat gcttgagtaa aacagcactt gttcgtgtgt cttcggctaa
     5041 aataaaatcg acttttttga gcacctctat agcccgaaat gtcatatcgt ccaaatttcc
     5101 tactggcgtg ggcactacaa atagtttcat gcaatttttt tatatatgag caaaacgttt
     5161 gtgtaatatt tctgaaaaat agacgtatgt actatcctct ttttgccaat caaaatgggc
     5221 atcaatatac tcttctacct cttgcaatga cgaacattga tttaatatat ctattgtctc
     5281 catcattttt tgttcatctc caccaaataa ttcgcgttta aaacgaaacc tatcattaat
     5341 accaatcgct ctacgcaaat cggttacaag tcgagcatcc acagcacttg ttttgggtgt
     5401 tgctaaaatg acagaagcag gcttaggggt ttccttatgg atggtcgttt ctggttgaat
     5461 actagatttt tgttgagtat acgtacgcgg ttcattttgt gaaggtggaa tagtaggtga
     5521 tgtgcttgac tttactgttg gttgggaaac tggttgctct tttttgcgtg tatccacaaa
     5581 ctcaatttct tcaatatcct catccagcaa aatagttgtg gtagtatcgt ctgtactggt
     5641 aatactatct tcttcatcgt taacatcagt aaactttgga gcacccttat ccgtaggcac
     5701 actatcttgc aataccaacg aaaagttttc ttccaaattt ttaaattttc tagttactaa
     5761 ttgttgcatt attggatgaa tcgtattcat tgtacgcaca ccattcacaa taaattcaag
     5821 ctctttgata tctttgagca tttggtcaat taaaatattt ttttccatga gccttttttc
     5881 tgaagaaatt aattattttt gcaaaagtag taattaaact caaaagcaac aaatgatata
     5941 tttagaaaca tcacgtcaat cagaaaaacg aagaggttca atcgaagtta tttgcggatc
     6001 tatgttctct ggaaaaaccg aagaacttat ccgtcgtcta aaacgggcaa aaattgcaca
     6061 attacatgta gagatattta aacctgccat agatgtgcgt tattcggaga cagaggttgt
     6121 atcgcacgat aaaaactcaa tattatcaac ggtggttgac tcttcacaaa atatattact
     6181 actttcaaat gatgttgatg taataggtat tgatgaggct caattttttg atatgggatt
     6241 aatagatgtg tgtagacaat tagccaatca aggaatgcgt gtaatcatag cgggtttgga
     6301 tatggacttt aagggtaatc cgtttggtcc aatgcctgct ttatgtgcta ttgctgaaca
     6361 tgttaccaaa gtaagagcta tttgtgttaa atgtggtcat ttagcccttt attcgtatcg
     6421 tttaacaaat tcggacaaac aagtgttgct cggagaaaca gccgactatc aaccattatg
     6481 tagagagtgc tatcacgaat ctacaacaaa actgaaagaa taaattacaa tcaacacatt
     6541 ttttgttgtt aataactttt aatgtataag tttcgttatc agccaattta cttgtatttt
     6601 tgcagaaaaa ggaataccat gaagcagtta gataacgcta atgaacggtt cgctcaactt
     6661 cttaaaaatt atgaatccaa tcttactgga ggctattttg atgcagaaga aatggagcaa
     6721 ttagctgatt tctatttgcg aagaatgcgt gtaaaggatg ctaaacgagc tattgaattt
     6781 gggctcaaac tccacccaaa caatattggg ttagaaacat ctaaagtacg tatcttaata
     6841 gaactgaacg atctggagct agctttgcaa cataccaatt cattagtagc tttatatccc
     6901 tctgatatag aattacatct actacaaacc gaaatattgc tcaaaaccaa gcaagaagta
     6961 caagcagaaa aaaactgcaa agagctaatt aatacctatg cacaagaaga tgaaacaatc
     7021 tatttggata ttgcttatgt gtatttaaat aatgaatatt accaaaaagc acttgctttc
     7081 ttacttattg gacacactcg aaatgtgtat aacatcaata ttttatttga aattgccttt
     7141 tgctacgaaa aacttaatca aatagacaat agcattgcaa cttaccatcg tattttagac
     7201 attgaaccgt attccgacga agcttggttt aatttggggc aggtctttta ttctcaaaaa
     7261 gactatgcaa aagccataga ggcatacgaa tatgctaccg ctataaatga agaagatgct
     7321 atggcatggc aactattggc acatgcttat taccaaaacg ataactacaa aaaagcagcc
     7381 gaaacctatt taatgtatgc tgagcaaaca gaacacaaag ctacaccact actatttgca
     7441 gccgaaagtt atgaaaaaat ggaagatttt gatttagctt tgcattatta cagtatggta
     7501 ctagatatgc aaccaaacaa tactgaggca atggtaggtg taggtatttg ccatttggaa
     7561 aaagaacaat ttcaaaaaag tatcaattat tttgtccgtg ccattgaatt agatccaaca
     7621 ctaagtgaag cttgggtata tttaggcgaa gcatatatca atttagatga cccttcaaaa
     7681 gggctacaag cctatttaac ttctatcgag ttaaacagca accaacccga aacattaact
     7741 agcattggaa atttatattt ggaacaagaa aacttttcaa tggcattaca ttactattta
     7801 acagcgtttg aattagatca tgaaacagac aatatacact tatttcttgc tatggcgtat
     7861 tacggtgaag aaaattatct gccagccaaa gaacatctac aaaaagctat caatgtaaat
     7921 actgaagcat taaatttatt tctagaaata tacccagatg cactacaata cattaatcta
     7981 acaacctagt agctggatta ttgcaaaaat taataaacgt acatacgcat actaaaaata
     8041 aaacaaaaat aaactaccat tatgaaacaa tacggtgtta taggaaatcc attacatcat
     8101 tcatactcaa aagcttactt taatgagaaa tttcaggaac aagctattga tgccaggtat
     8161 gacaattatt tactacccac cattaaagat gtaatgaaag tattgaatga tactcctaat
     8221 ttgtgtggtt taaatgtaac cataccatac aaagagcaag taatggaata tttggacgaa
     8281 attgatgacg aagcaaaaga gattggagct gtgaatgtaa taaaaattac agaaaaaaac
     8341 ggaaaacgtt ttttgaaagg atacaattct gattggtttg gctttacaga agctatcaaa
     8401 ccttttttga aaccccacca tacacacgcc ctaatattag gtacaggagg agcctcaaaa
     8461 ggaattctat atgctctaaa aaaattaaag attaatacac aatttgtatc acgagaccca
     8521 tctaaaggac ttacatacaa gcaactaaca aaagaaattt tgcaacaata cactattatt
     8581 gtg
//
LOCUS       DAOSRP010000087         8375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8050_length_8375_cov_4.908774, whole genome shotgun sequence.
ACCESSION   DAOSRP010000087 DAOSRP010000000
VERSION     DAOSRP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8050_length_8375_cov_4.908774"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..644
                     /locus_tag="PLJ82_06330"
     CDS             225..644
                     /locus_tag="PLJ82_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41158.1"
                     /translation="MKDRLKELLEKENLTAKEFAVLVDIQQSAVSHLLSGRNKPSMDV
                     IQKILRVFKHINPTWLILGDGGMYKSIKPIQGSLFEEKLSTHVNIEANTHQKDENTLK
                     NISEIPVNQVDKFLKTEKKIIRIVVYFSDNSYEEFNH"
     gene            791..1561
                     /locus_tag="PLJ82_06335"
     CDS             791..1561
                     /locus_tag="PLJ82_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOR41159.1"
                     /translation="MKKYILDCVVVANIPLNKRYFLLRVSHPHPFPRMLPGQFVELRV
                     DNSSDVLLRRPISIHDMNYETNEMDLLVQIVGAGTLKMSELKKGDLLNMIAPLGNSFS
                     MPTIDMKHVLLVGGGVGVAPLLLLGRVLQQYSCKVSFLLGARTASDLVQLDSFSQYGE
                     VYLTTEDATVGEKGFVTNHSLLKNKVFDKIYTCGPTPMMKAISVYAKTNNIECEVSLE
                     NTMACGFGVCLCCVTDTTEGHKCVCTEGPVFNLTTLKW"
     gene            1555..2463
                     /locus_tag="PLJ82_06340"
     CDS             1555..2463
                     /locus_tag="PLJ82_06340"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOR41160.1"
                     /translation="MVDLGINIGNLQLKNPVMTASGTFGYGTEFSDFFDISALGGIII
                     KGTTLHHREGNPYPRMAETPAGMLNAVGLQNKGVEYFITSIYPSLKTIDTHVLVNVSG
                     STIEEYVQAAEKINDLDKIPAIELNISCPNVKKGGMGFGTSCASAAEVVKAVRKVYTK
                     SIMVKLSPNVTNIAEIAQAVEAEGADSVSLINTLLGMAVDAEKQKPVLSTITGGLSGP
                     CVKPIALRMVWQVAKAVNIPIVGLGGIMNATDAIEFMLVGASAVQIGTANFIDPTVSL
                     KIVNGIEAYLQKHNYSSVKDIIGLIK"
     gene            2496..3884
                     /locus_tag="PLJ82_06345"
     CDS             2496..3884
                     /locus_tag="PLJ82_06345"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOR41161.1"
                     /translation="MRILILSYFLFVLHLVIYASDMPVAYARTLDDVLLYTNELQLTQ
                     DSLSNQSVLDTSVAIQYPDFELIINKDSLHLILNRILPAYQYVDSIRSDSVHIFEKML
                     IATIQHPYFVDWVFTRNGYKLQNEIEKQELINKIKSGNQVTIIKQADEVYVYELQNQQ
                     KLRKNTQLSFNDYVFINQDIKPRQISPSKQKVLPVYWTRGFQVGLHLSQHYISPNWYR
                     GGESHLALITLGSAFYNYNNKKTIQWENKLDFKAGFNSSTADSIRFLQTNEDVFRLTS
                     KFGYKAFTKFYYTGQTEFTTQLFNTYKVNTYDRITGAFSPIRLSVSGGLDYKESADFS
                     IFFSPVSYKYIYVNDTTKHISVAQSIADKLGIDKGKKSLHEVGSLVRINYRYKLSNEI
                     EFDTRTTIYTNYKGIEFDSEVNGNFKINRFLTTRLSVNPRYDSTFKLPNNDKARLQFR
                     QILSFGFSYKFY"
     gene            complement(3889..4563)
                     /gene="trmD"
                     /locus_tag="PLJ82_06350"
     CDS             complement(3889..4563)
                     /gene="trmD"
                     /locus_tag="PLJ82_06350"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR41162.1"
                     /translation="MRIDIITVLPELIESNFSHSIIKRGIEKGLAEVHIHNLRDYSTN
                     KHKRVDDYPFGGEAGMVLQIEPIDKVITELKNQRTYDEIIFTSPDGETFNQKMANELS
                     LVNNIIILCGHYKGVDHRVREHLITKEISIGDYVLTGGELAASIISDAIIRIIPGVIS
                     DEQSALSDCFQDNLLSAPVYTRPAIYNGWKIPEILLSGHEKKISEWRMEQALNRTKKL
                     RPDLLK"
     gene            4773..6797
                     /gene="ligA"
                     /locus_tag="PLJ82_06355"
     CDS             4773..6797
                     /gene="ligA"
                     /locus_tag="PLJ82_06355"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOR41163.1"
                     /translation="MNEIHQKILHLRKQLHQYNYEYYVLSQPTISDIEFDFLLKNLQA
                     LEELHPEYYDSNSPTQRVGSDSTTSFVQVSHQYPMLSLSNTYSEGEVRDFYERVSKSI
                     DEPFELVCELKFDGTSISLIYENGVLKQAITRGDGEKGDDVTNNVKTIRTIPLVLHGN
                     QYPAFFEVRGEIVMPWEVFDMLNKEREEQDEPLFANPRNATSGTIKLQNSSLVAKRNL
                     DAYFYAVLGNNLPANTHYDNLLRAREYGFKVSTDTKKCSSLQEVLEYIEYWDIARKQL
                     PVATDGIVIKVNSLTQQRFLGLTAKSPRWAIAYKFQAEKAVTRLNSVSYQVGRTGAVT
                     PVANLDPVLLSGTIVKRASLHNASIIASLDLYVGDSVFVEKGGEIIPKITGVDLDARI
                     LIGSKIQFITHCPECGSKLVNLPGEAAHYCPNEDDCPPQIKGKIEHFVSRKAMNIDGL
                     GKETIDLLYQQGLVKKVSDLYDLSAEQLSVLERLGEKSANNIIEAIEHTKQIPFERVL
                     FALGIRFVGETVAKKLAMAFHSIDKIQEASIQELVEVDEIGERIAQSVYEYLHNNKHQ
                     QLIQKLKDYGLQFTLAEDKLALKSTILAGKKFVISGTFSQFSRDELKDLIENNGGKNI
                     GSVSSQVDFIVAGDNMGPAKLAKAQKLNITLLTEQEFMAMLENGKTILKK"
     gene            6833..7372
                     /locus_tag="PLJ82_06360"
     CDS             6833..7372
                     /locus_tag="PLJ82_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41164.1"
                     /translation="MNQLQNILFKLRRYFLLKFHKKQWRDKKYLSFDNISSVALLYYI
                     DDYSKYEIVYKNIKDFTAMGKTVFSVLFVKKGVLAEEKRINMLLFTQNDCSWLCVPND
                     NIMLDALNMSADILIDISLMDSIPLQYIVANSQVGCKVAMKKENKFLYDLMIEMKEEQ
                     VNEDKLLETIAFYLKQIKS"
     gene            7510..>8375
                     /gene="dapA"
                     /locus_tag="PLJ82_06365"
     CDS             7510..>8375
                     /gene="dapA"
                     /locus_tag="PLJ82_06365"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOR41165.1"
                     /translation="MGVALVTPFKTDGSVDFSALARLVDYQLKNGTDYLVVLGTTAET
                     PTLSAEEQLEITQFIVEKVNGKLPIVLGMGGNDTRALVQKISTANLNGVDAILSVTPY
                     YNKPTQEGIYQHYAAVAKSTKLPIILYNVPGRTGVNMSVETTLRLANDFSNIIATKEA
                     SGNIAQVDAIIKNKPKDFLVISGDDAITFPLITLGASGVISVIGNAFPCEFSKMVRLA
                     LAGDYQTALTIHHGFTELIELLFVDGNPAGVKSILSAMGYLENQLRLPLLPTRITTYE
                     KMRNILLNMNIK"
BASE COUNT         2866 a         1199 c         1508 g         2802 t
ORIGIN      
        1 acaataaaaa ttgattatta ttatattgct ggacagcatt ttattgtaaa tatataaagt
       61 attagttttt atttattgat tgattttggt ggaatatgat actgaatgat aaatactatt
      121 ttttaatttg attttacaga tgtaaatcaa atggtactga aaatacagtt gtaatatttg
      181 caaatgtaat aaattgtaat tacatttgtg ataaaatata ttacatgaaa gataggttaa
      241 aagaactttt agaaaaagaa aatcttacag caaaagaatt tgcagtatta gtagatatac
      301 agcagtctgc tgtatctcac ctattgtctg gtagaaataa gcctagtatg gatgtgatac
      361 agaaaattct tcgtgtattc aaacatatta atccaacttg gctaatacta ggagatggag
      421 gtatgtataa gagtattaaa cctattcagg gctcattatt tgaagaaaaa ttatctacac
      481 atgtaaatat tgaagccaac acccatcaaa aagatgaaaa tacactgaaa aatattagtg
      541 aaatacctgt taatcaagta gataaatttt taaaaacaga aaaaaaaata attcgtattg
      601 ttgtatattt ttcagataat tcatacgaag aatttaatca ttgattttat ctactatcta
      661 ttttccaatt aagcgacttt ttttatcttt gtggaggttt ttacccatcc attatttgtt
      721 tgtaaacaag cgttgtttgt atatcagttt tgggtgtttt gattactaaa ttattctacc
      781 taatgaattt atgaaaaaat acattcttga ttgtgttgtt gtagcaaata tcccgttaaa
      841 taaacgctat tttctgttac gtgtatcaca tcctcatcct tttcctcgta tgttacctgg
      901 tcagtttgtt gaattgcgtg tagacaattc ttctgatgtg ttattaagaa gacctatttc
      961 tattcatgat atgaattacg aaacaaatga aatggatttg ttagttcaaa ttgttggagc
     1021 aggtaccctc aaaatgtcgg agttaaaaaa aggagactta cttaatatga tagctccctt
     1081 gggtaattca ttctcgatgc caacaattga tatgaaacat gtgcttttgg taggaggagg
     1141 tgtaggagtt gccccattat tattattggg aagggtatta caacagtatt cttgtaaggt
     1201 ttcatttctt cttggtgcac gtactgcgag tgatttagtt caacttgatt cttttagtca
     1261 gtacggcgag gtttatctta caacagaaga tgcgactgta ggtgaaaaag gttttgtaac
     1321 taatcattcg ctattgaaaa ataaggtttt tgacaaaatt tatacttgtg gtccaactcc
     1381 tatgatgaaa gccatttcag tatacgcaaa aactaataat attgagtgcg aagtatcgtt
     1441 agaaaatact atggcttgcg gttttggcgt atgtttatgc tgtgtaacag atacaacaga
     1501 aggtcataaa tgtgtgtgta cagaaggtcc agtgtttaat ttaacaacat taaaatggta
     1561 gatttaggta taaatattgg taatttgcag cttaaaaatc cagtaatgac tgcatccgga
     1621 acttttggat atggtacaga attttctgat ttctttgata tttcagcgtt aggtggaatt
     1681 attattaaag gaacaacgtt gcatcatcgc gaaggcaatc cgtatccacg aatggcagaa
     1741 actccagcag gaatgctaaa cgctgtcggc ttgcaaaata aaggagtaga gtattttatt
     1801 acatccattt atccatccct taaaacgatt gatactcatg tgttagttaa tgtttctggt
     1861 agtacaatag aagaatatgt gcaagctgca gaaaaaataa atgatttgga taagatacct
     1921 gctattgaat taaatatttc gtgtccaaat gtaaaaaaag gaggtatggg ttttggtaca
     1981 tcatgtgcat cagctgcaga ggtagtaaaa gctgtacgca aggtatatac caaaagcatt
     2041 atggttaaac tttctcctaa tgttaccaat atagccgaaa ttgcacaagc agtagaagct
     2101 gaaggggcag atagtgtatc gttgattaat acattgctgg gtatggctgt tgatgctgaa
     2161 aagcagaaac ccgttttatc aaccattacg ggtggattgt ctggtccatg tgttaaacct
     2221 atagctttac gtatggtatg gcaagtagct aaagctgtta acataccgat tgtgggctta
     2281 gggggtatta tgaatgcaac tgatgctatt gaatttatgt tagtaggtgc ttctgctgtg
     2341 cagataggaa cagcaaattt tattgaccca actgtgagcc tcaaaatagt aaatggaatc
     2401 gaagcatatt tacagaaaca taattacagc tctgtaaaag atataattgg gcttataaaa
     2461 taagtattta attttctttt attttcggtc tttttatgcg tattcttatt ttatcatatt
     2521 tcctctttgt gttgcatttg gttatttacg cttcggatat gcccgtggcg tatgctcgta
     2581 cactggatga tgttttacta tataccaatg agctacaatt aacccaagat tcattatcaa
     2641 atcaatctgt tttggatacg tctgttgcaa ttcaatatcc tgattttgaa ttgattatta
     2701 ataaagatag tttacatctt atattgaata gaatattacc agcctatcaa tatgttgatt
     2761 ctattcgctc tgattcagtt catattttcg aaaaaatgct catagctacc attcagcacc
     2821 cctattttgt agattgggta tttactcgaa atggatataa acttcaaaac gagatagaaa
     2881 aacaagaact tattaacaaa attaaatcag gaaatcaagt aacaatcatt aaacaagcag
     2941 atgaagttta tgtatatgag ctgcaaaacc aacaaaagtt gagaaaaaat acacagctgt
     3001 cgtttaatga ttatgtattt attaatcagg acattaaacc ccgtcaaatt tctccgagca
     3061 agcaaaaggt gttaccagta tattggacga gaggttttca ggtagggtta cacctttcac
     3121 aacattacat atctcctaat tggtatagag gaggggaaag tcatttagct ttgattacac
     3181 tgggtagtgc tttttataat tacaataata agaaaaccat acaatgggaa aataagcttg
     3241 attttaaggc tgggtttaat agctcaactg ctgattctat tcgatttctt cagacaaatg
     3301 aagatgtgtt tcgtttgact agtaaatttg gttataaagc atttactaaa ttttattata
     3361 ctggtcaaac agaatttacg acacaactct ttaacaccta taaggttaat acatatgatc
     3421 gtatcacagg tgctttttct cctatcagat tatcagtaag tggtggttta gattataaag
     3481 agagtgccga tttttccata tttttttcac ctgtttctta taaatatatt tatgtgaatg
     3541 atactacgaa acatatttcc gttgctcaat caatagcaga taaattagga attgataaag
     3601 ggaagaaatc gttacatgaa gtaggtagtt tggtgcgtat aaattatagg tataaattga
     3661 gtaatgagat tgaatttgat acacgtacta cgatatatac caactataaa ggtatagagt
     3721 ttgactccga agtaaatggc aattttaaaa taaatagatt tctaacaaca cgcctctcgg
     3781 taaatccacg gtatgattca acatttaagt tgccaaataa tgacaaagca cgtttacagt
     3841 ttaggcaaat attgagtttt ggattttcat ataaatttta ctaagttgtt actttagtaa
     3901 atctggacgt agcttttttg ttctatttag agcttgctcc atacgccatt cacttatttt
     3961 tttttcgtgt ccagataaca atatttcagg aattttccat ccattataaa tagcaggacg
     4021 tgtataaaca ggggcagaaa gtaagttgtc ttgaaagcaa tcagacaatg ccgattgttc
     4081 gtcagaaata acgccaggta ttatgcgaat gatagcatct gatattattg atgcagctaa
     4141 ttcgcctcct gtcagaacat aatctcctat agaaatttct ttagttatta aatgttctcg
     4201 tacacgatga tccactcctt tataatgacc acaaagtata atgatattat taacaagtga
     4261 aagttcgttc gccatttttt ggttaaaagt ttccccatct ggagaagtaa aaattatttc
     4321 atcgtatgta cgttgatttt ttagttcagt tattaccttg tcaataggct cgatttgcaa
     4381 aaccatgcca gcttcgccac caaacggata atcatctact cgcttatgtt tattggtaga
     4441 gtaatcacgt aaattatgga tgtgtacttc tgccaaacct ttttctattc ctcttttgat
     4501 aattgaatga gagaaattgc tttcaattaa ttcaggcaat actgtaatga tgtctatacg
     4561 catataacta caaatggatt ttcgacaaaa gtacgtaaaa attttcatac ttgtggattg
     4621 ttgaaaagta tatacataaa ataccaaatc aaaacacgat ttttattaca tttgtttact
     4681 caattgagtt gtaatactga atagcatttc tattgaatca tactagaaac aaatagtttc
     4741 ttattacttt atagttaaat aattatttga taatgaacga aatacatcaa aaaattttgc
     4801 acttgcgtaa acagttacat caatataatt atgagtatta tgtgctttct cagcctacaa
     4861 tttccgatat tgaatttgat tttttactga aaaacctaca ggcattagaa gaattgcatc
     4921 cagaatacta tgatagtaat tctcctacgc agcgggtagg aagtgatagt acaactagtt
     4981 ttgtacaagt ttcacatcaa tatccaatgt tatcattatc aaatacatat tcagaaggag
     5041 aagtacgtga tttttacgaa agggtatcaa aatctattga tgagccattt gagttagtct
     5101 gtgaattgaa atttgatggg acatctatct ctttgattta cgaaaatgga gtgttaaaac
     5161 aagccattac aagaggagat ggtgaaaagg gagatgacgt aactaacaat gtgaaaacaa
     5221 tacgtacaat tccactcgtg ttgcacggca atcagtatcc agcttttttt gaagtaagag
     5281 gagagattgt aatgccttgg gaagtatttg atatgctaaa taaagaacga gaggagcaag
     5341 acgaaccttt gtttgcaaat ccgcgtaatg caacctctgg aacaattaaa ttgcaaaatt
     5401 catccctggt agccaaaaga aatttagatg cttattttta tgctgttttg ggaaataact
     5461 tgcctgctaa tactcattat gataatttat taagagctcg tgagtatggg tttaaagtat
     5521 ctacagacac taaaaagtgc agttccttac aagaagtttt agagtatata gagtattggg
     5581 atattgctcg taaacaactc cctgtggcaa ctgatggaat tgttattaaa gtcaattcat
     5641 taacacagca acgtttcttg ggattaactg ccaaatcccc tcgatgggca attgcttata
     5701 agtttcaggc agaaaaggca gttactaggc tcaattctgt gtcatatcag gtaggtagaa
     5761 ctggagctgt aacacctgtt gccaatttag atccagtgtt attgtcagga actattgtta
     5821 aaagagcgtc gctacacaat gcaagtatta ttgcctcttt agatttatat gtaggtgata
     5881 gcgtatttgt agaaaaagga ggggagataa tccctaaaat aacaggagta gatttagatg
     5941 cacgaatttt gataggcagt aagattcaat ttattactca ttgtccagag tgtggctcta
     6001 agttagtgaa tttacctggg gaggctgcac attattgccc aaatgaagat gattgtccac
     6061 cacaaattaa gggaaaaatt gaacattttg ttagccggaa agctatgaat attgatggtt
     6121 tgggaaaaga aaccattgat ttattgtatc aacaaggttt agtaaaaaag gtatcagatt
     6181 tatatgattt atcagcagaa caattgtctg tattagagcg attaggcgaa aaatctgcca
     6241 acaatataat agaagctatt gaacatacaa aacaaattcc ttttgaacgt gtattatttg
     6301 cccttggcat tcgttttgta ggtgaaacgg ttgctaaaaa actagctatg gcatttcatt
     6361 ctattgataa gatacaagaa gctagtatac aggagttagt tgaagttgat gaaataggag
     6421 aaaggattgc tcaaagtgta tatgaatatt tacacaataa caaacatcaa caattgatac
     6481 aaaaattaaa agattatggt ttacagttta cacttgctga ggataaatta gctttaaaat
     6541 caactatatt agctggtaaa aaatttgtta tatcaggcac tttctctcag ttttcgcgtg
     6601 atgagctaaa agatttgatt gaaaataatg gaggtaaaaa tattgggtct gtatcctctc
     6661 aggttgattt tattgtggct ggagacaata tgggacctgc taagttggct aaagcacaaa
     6721 aattaaacat tacattacta acggaacaag agtttatggc aatgctcgaa aatggcaaaa
     6781 ctattttaaa aaaatagcat tatttaataa ggcaaaaatt atactaaaaa agatgaatca
     6841 attacaaaac attcttttca aattacgaag atatttttta ctcaaatttc ataaaaaaca
     6901 atggagagat aaaaaatacc tttcattcga caacatatct tctgttgcat tactctatta
     6961 tatagatgat tatagtaaat atgaaatagt atacaaaaac attaaagatt ttacagcaat
     7021 gggaaaaaca gtgttttctg tactgtttgt aaaaaaaggg gtacttgcag aagagaaacg
     7081 catcaatatg ttactcttta cccaaaatga ttgttcatgg ctttgtgttc ctaacgataa
     7141 tattatgctt gatgcgttga atatgtctgc agatatatta atagatatat cacttatgga
     7201 tagtattcct ttacaatata tagtagctaa ctcacaggtt ggttgtaagg ttgcaatgaa
     7261 aaaagaaaat aaatttttat acgatttaat gattgaaatg aaagaggaac aggttaatga
     7321 ggataaactt ttagaaacaa ttgctttcta tttaaaacag attaaatcgt aatgtataat
     7381 aattgattat acattgtaca aaattcccaa aaatcctttc ttatagtagc tgtttttgaa
     7441 taaaaaatac tatttttgca acttaaagaa aaaatacact ttttaatgga acgcaataaa
     7501 tttataggaa tgggtgttgc tttggtaaca ccttttaaaa cggatggcag tgttgatttt
     7561 tcagctttag ctcgattagt tgattatcag ctaaaaaatg gaactgatta tttagttgtg
     7621 ttaggtacaa cagctgaaac tcctacctta tctgctgaag aacagttaga aataactcag
     7681 tttattgtgg aaaaagtaaa tggaaagtta cctatcgtat tgggtatggg cggaaatgat
     7741 acacgtgcgt tggtacaaaa aatttcaaca gcaaatttaa atggtgttga tgcaatttta
     7801 tcagtaactc cttattataa caaacctaca caagaaggta tttaccaaca ctatgcagca
     7861 gtagccaaaa gtacaaaatt gcctatcata ttgtataatg ttccaggtcg tacgggtgtt
     7921 aatatgagtg tcgaaactac gttgcgtttg gcaaatgatt tttcaaatat cattgccacc
     7981 aaagaagcat caggaaatat agctcaagta gatgcgatta ttaaaaataa acctaaagat
     8041 tttttagtta tctctggtga tgatgcgatc acatttccgt tgataacctt aggtgcttca
     8101 ggtgttattt cggttattgg taatgctttt ccttgcgaat ttagtaaaat ggttcgctta
     8161 gccttagctg gtgattatca gacagcttta accattcatc atgggtttac tgaattaatt
     8221 gaactattgt ttgtcgatgg taatcctgct ggagtaaaaa gtattctatc tgcaatgggt
     8281 tatttagaaa accaattacg attaccttta ttaccaacaa ggataacaac ctacgagaaa
     8341 atgcgaaaca ttcttttaaa tatgaacatt aaatg
//
LOCUS       DAOSRP010000088         8302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8150_length_8302_cov_4.625440, whole genome shotgun sequence.
ACCESSION   DAOSRP010000088 DAOSRP010000000
VERSION     DAOSRP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8150_length_8302_cov_4.625440"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLJ82_06370"
     CDS             <1..620
                     /locus_tag="PLJ82_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41166.1"
                     /translation="TTEVYYKNMTNVISYTIDNVKVTYSGKNDAKAYAAGIDMKLFGE
                     FVPGVDSWLSVSIMNTKEDIDDDGYGYLARPMGQLYNFSIFFQDYVPKFPAYRVHLLF
                     NWSQGFPVTLARGERFYSNARMPDYRRVDIGASRIFDKKEDTWMLKPAFKHIERMHIG
                     IEVLNLFGFYNVNSYYWVTDVFNQQNAVPNYLTGTQLNVKLQIDF"
     gene            638..1870
                     /locus_tag="PLJ82_06375"
     CDS             638..1870
                     /locus_tag="PLJ82_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOR41167.1"
                     /translation="MYNIVFFLIVLFIVADFLLERVLALLNAKKIGIAIPPILEGIYD
                     NEKYQKQQRYSLVNYKFGIFTSSFNVFILLIMFAFGLFGLLDGVLRGVIENELLLTLV
                     FFGVLFFANDILNTPFDYHQTFVIEEKFEFNKMSKGLFFIDKLKGIFLAVIIGGAILS
                     VITLIYQWLPNYFWLLAWAVIAFFNVFLMMFYSEWIVPLFNKQTPLEEGELRTKIEEF
                     SKKADFDLVNIFVIDGSKRSTKANAYFSGLGAKKRIVLYDTLIHELTADEIVAVLAHE
                     IGHYKKKHTLVMLFLSLINMLVMFAVLGWFLGSPLIAQALGSTISSFHLGLLAFGILY
                     SPVSTVLSLGVNMLSRKNEYEADAYAAQFGLSEYLISALKKLSAKSLSNLQPHPAYVF
                     VHYSHPTLLQRINALTQK"
     gene            1872..3134
                     /locus_tag="PLJ82_06380"
     CDS             1872..3134
                     /locus_tag="PLJ82_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOR41168.1"
                     /translation="MLHKVAVDIITIGDELLIGQIVDTNSAYIAKELAKNGFSTEHMY
                     SVSDKKEAIIEAIQRSFSQVDIVLLTGGLGPTKDDITKTVLCEYFQTKLVFSQDVYDD
                     VQELLSYRPNAMNGLNRQQAFVPQSCTVIRNKVGTAPIMWFEKNKKILVSMPGVPNEM
                     KWAMEHAVMDKLKRFFQLPTILHQHIIVYGYPESVLADTLTDWENNLPNSISLAYLPS
                     ISHVKLRMSAIAEDATLLSIQMQKQVELLKTILGKAIIALEDKPLEELLSNWLIDNNL
                     TLSVAESCTGGNIARLVTSISGSSAYFKGGVIAYANEIKEQVLAVSTHSLKEYGAVSQ
                     QVVEQMAKGVMQLTGSDVAIATSGIAGPSGGTENKPVGTVWVAVCNRHTVKSHCFSFG
                     GIRERVIERASYAGIIMLKELIEYTSLK"
     gene            complement(3109..4089)
                     /locus_tag="PLJ82_06385"
     CDS             complement(3109..4089)
                     /locus_tag="PLJ82_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOR41169.1"
                     /translation="MTPLYPFKFSPIFQERIWGGQNLKHTLGKVFDNQFIGESWELSG
                     LPNNQSVVANGMLIGKTLTELIETYKHDLVGNIIYQRFGSSFPLLFKYLDACDNLSVQ
                     VHPSNEIAQKKHQCDGKTEMWYVVSASDNAQIIAGVKDNIELKDIKQSIEKNTIEDLL
                     HFHDAKQGDVFHITEGIIHALGKGTIVAEIQQSSDITYRVYDYNRKDQHGKSRDLHIN
                     DALDCIVLNDVNKTHADFSETDIQQELVSCPFFTTNLLSITQPTSFNYQAIDSFVVYM
                     SIEGDYVVETSFSSLDVKKGDTILIPSSVKDIRIIPKDGGKALILETYIQ"
     gene            complement(4100..5104)
                     /locus_tag="PLJ82_06390"
     CDS             complement(4100..5104)
                     /locus_tag="PLJ82_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR41170.1"
                     /translation="MGNQQIVLSANQLCIGYKQRKLLDNIHIELQKGEVVCLIGKNGC
                     GKSTLIRTLCGLQPSLSGSICINNQSIYQLNHHQKAQLISVVLTDKVQADSLTVFEIV
                     SLGRYPYTNWMGSITDEDKNSISDAISLVHLEHKTHELYAKLSDGEKQRVMIAKALTQ
                     DTPVIFLDEPTAHLDLPNRMEIMALLQKLAKNTEKAILLSTHELDLALQTADRLWLMD
                     EQRIFVGTPEDLVLNGIVEQNFQSNLFQFNRKHGNFCMKHQSMTHFVEVHGDTYLVYW
                     TMRALNRHGIGQQKNAPIQVYVEKDKWIISKEKVKNEFSSIENLVNHLKLKNNLYFCV
                     "
     gene            complement(5108..6124)
                     /locus_tag="PLJ82_06395"
     CDS             complement(5108..6124)
                     /locus_tag="PLJ82_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOR41171.1"
                     /translation="MPLRTTIIFFLLLMILVFVFLMDILLGSIQIPIQKIFSVILTNH
                     HDGYSEIIWQFRLPKALTATLVGSSLAVSGLLMQTLFKNPLASPYVLGISSGASLGVA
                     LLIMASGASWIPIFMVTSGWGQIFAAIIGALLILMLVLLFSTRINDTVSLLIVGMMFG
                     SITGALVNVLQSITNPDALKIFVIWTMGSLSAVSWEYMYIMFPMLFAGLLISLLLPKQ
                     LNALLLGENYAKSIGVTVFRLRFIIILVTCLLAGAATAFTGPIAFIGVAVPHIIRGIF
                     RSSDHRIILPGSILGGAILLLMCDIVSQLPGSEYTLPINSVSAIIGAPIIIWIIFKNK
                     NLYS"
     gene            complement(6133..7275)
                     /locus_tag="PLJ82_06400"
     CDS             complement(6133..7275)
                     /locus_tag="PLJ82_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR41172.1"
                     /translation="MRNIFFYTLIFFVICYTATGCTKQQKQDSLDSTTPIRSVPISYA
                     TNFSIDHFETYTRVIVYNPWQQNTILQTYYLVENISTTTPDNGIKIQIPIHTLVVNSC
                     SHIEFICMLNELESIVGISSPELVFNPIIRQRAANGKIENMGDAFAMNIEKILIKKPN
                     AIMVTGYNQMSDSHLRLETTHIPIIVNIEWMEASSLGRAEWIKFIAAFYKQEEIADSL
                     FQQVEKNYKELLALAQTVPSKPSIASGNSFKGTWYLPGGNSFMGKLYYDAGGDYVFAK
                     DSSSGSLPLSFEVAMKDLRHCDIWLGSNANSLHELVQMDSRLMLFDAYKNKQVYNYSK
                     RTTPSGGNDYWESAIARPDILLKDLIKVLHPNLLPDYELFYINRLE"
     gene            7570..7803
                     /gene="rpmB"
                     /locus_tag="PLJ82_06405"
     CDS             7570..7803
                     /gene="rpmB"
                     /locus_tag="PLJ82_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR41173.1"
                     /translation="MSKICQITGKSAMVGNNVSHSKRRTKRTFDINLFTKKFYWVEQD
                     AWISLKISANGLRVINKLGLNAAIKRAAEKGYL"
     gene            7825..8013
                     /gene="rpmG"
                     /locus_tag="PLJ82_06410"
     CDS             7825..8013
                     /gene="rpmG"
                     /locus_tag="PLJ82_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOR41174.1"
                     /translation="MAKKAKGNRIQVILECTEHKTSGMPGTSRYISTKNRKNTTERLE
                     LKKYNPILKKVTVHKEIK"
     gene            8026..8178
                     /locus_tag="PLJ82_06415"
     CDS             8026..8178
                     /locus_tag="PLJ82_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOR41175.1"
                     /translation="MAKKTVASLKKTDGRAYAKVIKMVKSPKTGAYIFEEDIVLNDKV
                     KDFFGN"
BASE COUNT         2698 a         1330 c         1426 g         2848 t
ORIGIN      
        1 ttacaacgga ggtatattat aaaaatatga caaatgtaat atcatatact attgataatg
       61 taaaagttac ctattcgggt aaaaatgatg ctaaagcata tgctgctgga attgatatga
      121 aattatttgg agaatttgtg cctggggttg attcgtggct tagtgtttct attatgaata
      181 caaaagaaga tattgacgat gatgggtatg gttatctcgc tcgaccgatg ggacaattat
      241 ataatttttc tatctttttt caagattatg tacccaaatt tcctgcatat agagtacatt
      301 tgttattcaa ttggtcgcaa ggttttcctg ttaccttagc acgcggagag cgtttttata
      361 gcaatgctcg tatgcccgat taccgtaggg tagatattgg tgcatctcgt atttttgata
      421 aaaaagaaga tacttggatg ctaaaacctg catttaaaca catcgaacgt atgcatattg
      481 gaatagaagt gttaaatttg ttcggttttt ataatgtaaa ctcgtattat tgggtaacag
      541 atgtatttaa tcagcaaaat gctgtgccta attaccttac tggaacccaa ctgaatgtta
      601 aattgcaaat agatttttaa attttaatta ctaaagtatg tataatatag ttttttttct
      661 aatcgttctt tttattgtag ctgatttctt attagagaga gtattagccc tgttaaatgc
      721 aaaaaaaatt ggcattgcta taccacctat cttagagggt atttacgaca atgaaaaata
      781 ccaaaaacaa caacgttatt cattagtaaa ctataagttc ggaattttta ccagtagttt
      841 taatgtgttt atattgttaa tcatgtttgc tttcggtttg ttcggtttgt tagatggtgt
      901 gttacgtggt gttattgaaa acgagttatt gctcactctt gtattttttg gagtattatt
      961 ttttgcaaat gatatcttga atacgccttt cgattaccat caaacttttg ttatcgaaga
     1021 aaaatttgag tttaataaaa tgtctaaagg tctatttttt atcgataagc tcaaaggtat
     1081 ttttttagct gtaattattg gaggggctat tttgtcggtg ataaccttaa tttatcaatg
     1141 gttgccaaat tatttttggc tattagcttg ggcggttatt gcttttttta atgttttctt
     1201 aatgatgttt tattctgaat ggattgtccc tttatttaat aaacaaactc cattagaaga
     1261 aggagaattg agaactaaga ttgaagaatt ttccaaaaaa gctgattttg atttggtaaa
     1321 tatattcgtt attgatggct ctaaacgtag tacgaaagca aatgcctatt ttagtggttt
     1381 aggtgctaaa aagcgtattg tattgtatga tacacttata catgaattaa ctgctgatga
     1441 gatagttgca gtgttagcac acgagatagg tcattataaa aagaaacata ctttagtaat
     1501 gttattttta tcacttatca atatgctcgt tatgtttgct gttttaggct ggtttttggg
     1561 ttcgccatta atagctcaag ccttaggtag cactatttcc tcttttcatt tgggattact
     1621 tgcttttggt attttatatt caccagtttc tactgtattg agtcttggtg ttaatatgtt
     1681 atcacgcaaa aatgaatacg aggcagatgc ctatgctgct caatttggct tgtctgaata
     1741 tttaataagt gctcttaaaa aattatcggc aaaatcattg tctaatttac aaccacatcc
     1801 agcatatgta tttgtgcatt attcgcatcc tactttgttg caacgtatta acgcattaac
     1861 tcaaaagtaa aatgttacac aaagtagctg ttgatattat taccattgga gatgagttgt
     1921 tgatagggca gatagtcgac acaaattcag catatatagc taaagagctt gcgaagaatg
     1981 gattctccac cgaacatatg tattccgttt ctgataaaaa agaagccatt attgaagcta
     2041 ttcaacgttc tttcagtcag gtagatattg ttcttttaac aggtggattg ggaccaacta
     2101 aagatgatat tactaaaacc gtgttatgcg aatattttca aactaaactc gtttttagtc
     2161 aagatgtgta tgatgatgtg caagagttac tttcttatcg cccaaatgcg atgaatggtt
     2221 taaatcgaca acaagctttt gttcctcaat catgtactgt tattcgtaac aaagttggta
     2281 cagctcctat tatgtggttt gagaaaaata aaaaaattct tgtatccatg cctggcgtgc
     2341 caaatgaaat gaaatgggca atggaacatg ctgtaatgga taaattgaaa cgtttttttc
     2401 aacttccaac tattcttcac caacatataa tagtgtatgg ctatccagaa tcagtacttg
     2461 cggatacgct tacagattgg gagaataatt tgcccaattc aatctctcta gcttatcttc
     2521 ctagtatatc tcatgttaaa ttacgaatga gtgccattgc agaagatgca acgttactct
     2581 ccattcagat gcaaaaacaa gttgaattgt taaaaacaat tttgggaaaa gcgatcattg
     2641 cattagaaga caaacctctt gaggaattat taagcaattg gcttatagat aataatctta
     2701 cactttctgt tgctgaaagt tgtacagggg gcaatattgc ccgtttagta acatcaatat
     2761 caggtagttc tgcttatttt aaaggaggag ttattgcata tgcaaatgaa ataaaagaac
     2821 aagtcttagc cgtttctact cacagtttga aagaatatgg ggctgtaagt cagcaggttg
     2881 tagaacaaat ggcaaaaggg gtgatgcaat taacaggtag cgatgtagct attgcaacat
     2941 caggaatagc tggtccaagt ggcggtactg agaataaacc agttggaacc gtttgggtag
     3001 cagtatgcaa tcgacataca gtaaaaagcc actgtttttc gtttggtgga atcagagaac
     3061 gtgtgatcga aagagcctcc tatgcaggga taattatgtt aaaagagctt attgaatata
     3121 cgtctctaaa ataagtgctt ttcctccgtc cttagggata atgcggatat cttttaccga
     3181 tgatggtatg aggattgtat ctcctttttt tacgtccaaa cttgaaaagg atgtttctac
     3241 cacataatcg ccttcaatac tcatatatac cacaaacgaa tcaatagcct gatagttgaa
     3301 tgaagtaggt tgagttattg aaagtaaatt agttgtaaaa aatggacagc ttactagctc
     3361 ttgttggatg tctgtttctg aaaaatcggc gtgcgttttg ttgacatcat tcaatacaat
     3421 acaatctaaa gcatcgttaa tatgtaaatc tcttgatttt ccatgttgat cttttctgtt
     3481 gtagtcatat acccgataag tgatatccga tgattgttgt atttctgcta caattgtacc
     3541 tttgcctaat gcatggataa tgccttctgt aatatgaaat acgtcacctt gtttagcatc
     3601 atgaaaatgc aataaatctt caatagtatt cttttctatg ctttgtttta tatcttttag
     3661 ttcaatgtta tcttttaccc ctgctataat ttgtgcatta tcagaagcag aaactacata
     3721 ccacatttca gtttttccat cacattggtg ttttttttgt gctatttcgt tgctgggatg
     3781 cacttgtacg gataaattat cgcaagcatc taagtattta aatagcaaag ggaatgacga
     3841 cccgaatcgt tgatagatga tattgccaac caaatcgtgt ttatatgttt ctattagttc
     3901 tgttaatgtt tttccaatta acataccatt tgccacaact gactggttgt tgggcaagcc
     3961 agaaagttcc caactttcgc caataaattg attatcaaaa actttaccaa gagtatgttt
     4021 tagattttga ccaccccaaa tacgttcttg aaaaatgggg gagaatttaa atggatataa
     4081 tggtgtcata gtaactctat tatacacaaa agtacaaatt gtttttaagt tttagatggt
     4141 ttactagatt ttcaatactc gaaaactcat ttttaacctt ttcttttgaa ataatccatt
     4201 tgtctttttc tacatatact tgaataggtg catttttttg ttgtccaatc ccatgtctat
     4261 ttagggcacg cattgtccaa tataccaaat aggtatcacc atgaacctct acaaagtgtg
     4321 tcatagattg gtgtttcata caaaaattgc catgtttgcg attgaattga aataaattcg
     4381 attgaaagtt ttgttctaca attccattta acactaaatc ttctggtgtt cccacaaaaa
     4441 ttcgttgctc atccattagc catagtctgt ctgcagtttg caatgccaaa tctaattcat
     4501 gagtggatag taaaatagct ttttcggtgt ttttagctaa tttttgtagc aatgccataa
     4561 tttccatcct atttggtaaa tctaagtggg cagtaggttc atctaaaaaa ataacgggtg
     4621 tatcttgggt taaggctttt gcaatcatta ccctttgttt ttctccatct gataatttag
     4681 catacaattc gtgtgttttg tgttctaaat ggacgaggga gatggcgtca gagatactat
     4741 tcttgtcttc gtcagttatg cttcccatcc agtttgtata gggatatcta cccaacgaaa
     4801 ctatttcaaa aacagttagt gaatcggctt gtactttatc ggttaatacg acacttatta
     4861 gttgagcttt ttgatgatga tttagttgat aaatagattg gttgttaata cagatacttc
     4921 cagatagaga aggttgtaag ccacataatg tgcggataag tgtagattta ccacaaccat
     4981 ttttaccaat taaacagact acttctcctt tttgtaattc aatgtgtata ttgtcaagta
     5041 gctttcgttg cttatatcca atacatagct gatttgctga taaaacaatt tgttgattac
     5101 ccattgttta agaatataag tttttatttt taaaaattat ccaaataata attggagccc
     5161 caataatagc acttacagag tttattggta aggtgtattc actcccaggt aattgagaaa
     5221 cgatatcgca cattaataat agtatggctc cacccaatat actgccaggt aaaataattc
     5281 tgtggtcaga acttctaaat attcctctta taatatgtgg tacagccaca cctataaagg
     5341 ctataggacc agtaaaagcg gttgcagctc ctgccagtaa acaggtaact aatatgatga
     5401 taaaccgcaa tctaaaaaca gtaactccaa tactttttgc ataattctct ccaagtaata
     5461 atgcgtttaa ttgttttggt aatagtaaag aaataagaag cccagcaaat aacattggaa
     5521 acataatata catatactcc caacttacag cacttaaact gcccattgtc caaatcacaa
     5581 atatttttag agcatctggg tttgtaatac tttgcaatac atttaccaat gcacccgtaa
     5641 tactaccaaa catcatccct acaattaata atgaaacagt atcgttgatg cgtgttgaaa
     5701 ataacagtac cagcattaaa ataagtaaag ctcctattat agctgcaaaa atctgccccc
     5761 aaccactcgt taccataaaa attggtatcc acgatgcacc tgaagccata atcaataaag
     5821 ccactcccaa acttgcacca gagcttatac ccaacacata gggacttgct aaaggatttt
     5881 taaacagagt ttgcattaat aatccagaaa cagctaaaga cgaccctact aacgtagccg
     5941 tcagggcttt cggtaatcga aactgccaaa tgatttcgct gtatccatcg tgatgattgg
     6001 tcaagattac agaaaatatt ttttgtatag gtatctgaat acttcccaaa agtatatcca
     6061 taagaaaaac aaataccaat atcataagta atagaaaaaa tataattgtt gtacgaagtg
     6121 gcatgaatgt gtttattcta atcggtttat ataaaataat tcataatcag gtaataaatt
     6181 aggatgaaga acttttatta aatcttttaa taaaatatct ggacgtgcaa ttgctgattc
     6241 ccaataatca tttccaccac taggtgtagt acgtttgcta taattatata cttgtttgtt
     6301 tttataggca tcaaatagca tgagcctgct atccatttga actaattcat gcaatgagtt
     6361 tgcgtttgac cctagccata tatcacaatg tcgcaaatct ttcattgcca cttcaaagct
     6421 caaaggtaaa ctacccgaac tcgaatcttt tgcaaacaca taatcccctc cagcatcata
     6481 atataattta cccataaaac tattgccccc aggtaaatac catgtacctt taaaactatt
     6541 gccagatgct atgctaggct tagaaggtac agtttgtgcc agggctaaca attctttata
     6601 gtttttttct acttgttgaa agaggctgtc tgctatttct tcttgtttgt aaaatgccgc
     6661 tataaattta atccactctg ccctgcctaa actggaggct tccatccatt caatatttac
     6721 aatgattgga atatgtgtag tttctaatcg taggtgtgaa tcactcatct gattatagcc
     6781 agttaccata atagcattag gcttttttat caaaattttt tctatgttca tcgcaaacgc
     6841 atcacccata ttttctattt taccatttgc tgcacgttgt ctaataatag gattaaatac
     6901 aagttcagga gaactaattc ctacaatgct ttcaagttcg tttagcatac agataaattc
     6961 aatatgacta cacgagttga caacaagggt atgtattgga atttgtattt taattccatt
     7021 gtcaggagtt gtagttgata tattttctac caaataatat gtttgtaaga tggtgttctg
     7081 ttgccaagga ttatacacaa ttacgcgtgt ataggtttca aaatgatcaa tagaaaaatt
     7141 tgttgcatag gaaataggaa cagaacgaat gggtgtagtg ctatctaggg agtcttgttt
     7201 ctgttgtttt gtgcaacccg tcgcagtata acaaattacg aaaaaaataa gtgtgtaaaa
     7261 aaaaatattc ctcatcaatg cataaattag catacaaagt tacacttttt tatcaaatct
     7321 tatttttaca actgaatttt tcttgttttt acgtctggta tatctattta aatgtcataa
     7381 aaatttggtt atatagataa aaaatgcgaa atttgcaccc gcttataaga acaaaacacg
     7441 cttataagac ttgagtattg aatcattcag gctttttgtt gataaattct tgaatgtttc
     7501 ttttgttatg tgcaaaaaaa gtattttctt tgcactctat ttttaaaaac aacaattaag
     7561 gatacagcga tgtcaaaaat ttgtcaaatt acaggaaaaa gtgccatggt tggcaacaat
     7621 gtttctcact caaagagaag aacaaaaaga acattcgata ttaacctttt tacaaaaaag
     7681 ttttattggg ttgaacaaga tgcgtggatt agccttaaaa tttcagctaa cggacttcgt
     7741 gtaataaaca agctaggttt aaatgctgca attaaaagag ctgctgaaaa aggttattta
     7801 taataaatat agggagatac gaaaatggca aaaaaagcaa aaggaaatag aatacaagtt
     7861 attcttgaat gcaccgagca caaaactagt ggtatgcccg gaacttctcg ttacatttca
     7921 actaagaaca gaaaaaatac aactgaaaga ttagaattaa aaaaatataa tccaatcttg
     7981 aagaaagtta cagtacataa agaaattaaa taaatagtat aagtcatggc aaagaaaacg
     8041 gttgcgtctc ttaaaaaaac agatggacgt gcatacgcaa aagtgattaa aatggtaaaa
     8101 tctcccaaaa caggagctta tatctttgaa gaagatatcg ttttaaatga caaagtgaaa
     8161 gatttttttg gcaattaagc taatttaaac catatttcaa taacctctta agtttttgct
     8221 tgagaggtta ttttgtatat ataggtatat tttttgtatc tttgtctatc caatttttgt
     8281 ttcacagcaa ttcgtacatt ta
//
LOCUS       DAOSRP010000089         8138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8349_length_8138_cov_5.255722, whole genome shotgun sequence.
ACCESSION   DAOSRP010000089 DAOSRP010000000
VERSION     DAOSRP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8349_length_8138_cov_5.255722"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..239)
                     /locus_tag="PLJ82_06420"
                     /pseudo
     CDS             complement(39..239)
                     /locus_tag="PLJ82_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783987.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
     gene            471..1454
                     /locus_tag="PLJ82_06425"
     CDS             471..1454
                     /locus_tag="PLJ82_06425"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOR41176.1"
                     /translation="MKKKIAIIAGGNSSEVIVSLKSAQGIYSFIDKKTYEIYIVTLIE
                     KKWSVKLEDETEVDIDKNDFSFILNGEKILFDFAYITIHGTPGENGILQGYFDLIGLP
                     YSCCGVLAAAITFNKFTCNQYLKGFGIPVAPSILLRKNEHIQNTVVVEKIGLPCFIKS
                     NVGGSSFGVTKVKEEKQIQPAIEKAFAEGNEVIIEGFLQGTEVTCGMYKTHEKTQVFP
                     ITEVVSANEFFDFDAKYNGQVTEITPARIPQEVTNHIQQLTNQLYDILGCKGIIRADF
                     IINKQNVPYLLEVNTTPGMTPTSFIPQQVKAAGMAISSVMEDIIENKLNHL"
     gene            1478..2452
                     /locus_tag="PLJ82_06430"
     CDS             1478..2452
                     /locus_tag="PLJ82_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR41177.1"
                     /translation="MKSREEIIEIVQNELTLTNWLSEPIDLYKPIAYTMSMGGKHIRS
                     AITLMACNLYSDNIHLALQPAIGIEVFHNFTLLHDDIMDKAETRRGKPTVHVKWNDNT
                     AILSGDAMLIKAYQLLAKCPPENLSEILSVFSQTAIEVCEGQQYDMNFEHQDTVSEEE
                     YIEMIRLKTAVLLAASLKIGAIIGGATPLQANALYDFGIHIGIAFQLQDDLLDVYGDS
                     TIFGKKIGGDIICNKKTFLLIRALKNANNTQRNKIQHWMTQHPDDPTEKIVQMTSIFN
                     ETGVKQACQQRMKEHYNKALLALNKACEVEEKIVHLQKLASELMQREK"
     gene            2522..3235
                     /locus_tag="PLJ82_06435"
     CDS             2522..3235
                     /locus_tag="PLJ82_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41178.1"
                     /translation="MPYRRLPNTDAARLRALRAVACYKNSPIDTELPFDRRILQEICS
                     FLPQFENAMFEYKQAINSEGNKNNKYQQYIKNVRLYISHFIQVLNLACIRNEIKPEKK
                     LLYKLLPDNYAVPDLSTETLLLEWGKNIIQGENERTRAGGSPIYNPTIAKVQVHYDIF
                     KEAYHSKKVAQKNVGRYAENMQKMRMRADEIILDAWNQIEQAFVHYGMDAMQQKAKEF
                     GIVYYLRRKERQAMTKKSH"
     gene            3363..3812
                     /locus_tag="PLJ82_06440"
     CDS             3363..3812
                     /locus_tag="PLJ82_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41179.1"
                     /translation="MKKFILLLTIFILSSFVAFSAELPSKGYSGSSFSDGGSFMDEPT
                     LSVGNRIGATNSVLGVNACQKACSDKLMIESVACGGNIICLEACFIAYTACMDACETY
                     SLPIGSGFFFLLLLSGGYAGTKLNKLLFIMVWMRCNKKQKNLELSIT"
     gene            3982..4383
                     /locus_tag="PLJ82_06445"
     CDS             3982..4383
                     /locus_tag="PLJ82_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41180.1"
                     /translation="MKKYILLLTIFILSSFVAFSAKLPSKGYSGASFSDGGSFMDEPT
                     LSVGNRIGATNSVLGDGVDCIDACGETLEFAIAECNGDDVCEDMALTAFGTCVDACET
                     YSLPIGSGFFFLLLLSGGYAGVKLRRKRKEA"
     assembly_gap    4511..4520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4704..7676)
                     /locus_tag="PLJ82_06450"
     CDS             complement(4704..7676)
                     /locus_tag="PLJ82_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOR41181.1"
                     /translation="MDKTLNLQKLYFKDTSFVSLMKERIYNILLIASKYDAFMLEEDG
                     RIDELIFNEYTALNLRYPPRFTIASEHKEVKELLQTRHFDLIIAMPTSDNSHILEMAN
                     ELKVSYADIPIVVLTTLSRRIFKRVFSEGDFLHVDYVFSWLGNSDLLLAIVKLLEDKL
                     NVDDDVTSVGVQVILFVEDSIHFYSSILPYLYKHVFTQSLSFMTEALNAHQQMLRMRG
                     RPKILLARTYEEAMFLYKKYQKNMLGVITDVRFPRAGQIHPTAGLELSQEIRKLDPFV
                     PIIVESSEPKGVCEQTDRLCAVFLDKTDKTFHKQFKDAITHHFKFGDLVFVSPDTREA
                     LITVHNLQDLQKHIFDIPANSLFYHLAQNDLSRWLYSRAMFPLAEYLRYIIIKDIKDI
                     DRARHIVFDAIVNYRKIKNKGIVAVFQSDRFDEYSNFARIGEDSMGGKGRGLAFLNAV
                     SKRNPTLYDFENTQITVPKTVVLCTDMFDEFMETNNLYVVALREDVSDEQILEVFLEA
                     RLPRKIMKDLYAFLGVIQSPIAVRSSSLLEDSHYQPFAGIYSTYMVPNVSESRTTTLR
                     MVCDAIKAVYASVFYADSKAYMKATKNLIDEEKMAVVLQEVCGNAYGNRFYPSFSGVA
                     RSLNYYPIGEEKPEEGVVNVALGLGKYIVDGGQTLRFCPAYPNKVLQTSTMEFALSET
                     QNYFYCLDLAQRSFKPRVDDGDNLQKLHIREAEQDGTLRWLASTFDPIDQVIRDGVYA
                     GGRKLITFVNILEHQSFQLADILKNVLTVGEKEMGCPVEIEFAVHLDYEKNEQHTFYL
                     LQIRPIVGLNESLTEDISLISKEKTFLSTTNALGNGIMNDVYDVVYVKKGAFNPSKNI
                     LIAYDIEKINKQLVAEDKSYILVGPGRWGSSDHWLGIPIKWPHISGARVIVEAGETSY
                     HIDPSQGTHFFQNLTSFGVCYFTITQNEDDYYDCAYLDEQEALHETEYIRHVRFSSPL
                     LVKVDGRKNRGVVLKVES"
BASE COUNT         2764 a         1506 c         1345 g         2513 t
ORIGIN      
        1 tattagcaag ctttgacagt ttagaaaggg catataaata cttcaatatg tttacaatgg
       61 ccagcaagtg cctcgtcaat tgaattatca accacctcat atacaaggtg atgcaacccc
      121 ttcgtcccaa tatcaccaat atacatagcc ggacgcatac gtactgcctc caacccttct
      181 aatacagtaa tactactggc tccataccca ccattttctt ttttttcttc ttcgctcata
      241 atttttaaat taagtaaaaa ataaacagac aaatttacat aaacttatca agaaagcgaa
      301 attttacaga cacaaaatga ctctaaaatg gtaatttaga ttttcccttt tttattctct
      361 tattcatcaa tcaaataaaa aaatccttgt atatttgcac tttcttatgt ctttgcctta
      421 taaccaacat agtattattt agtttatatc attagcttca acatttactt atgaaaaaaa
      481 agattgctat cattgctgga ggaaactctt ccgaagttat cgtttctctt aaaagtgctc
      541 aaggtattta ctcttttatt gataaaaaaa cctacgaaat atatattgtt accttaattg
      601 agaaaaaatg gtctgtcaaa ttagaagatg aaacagaagt cgacatagac aaaaacgact
      661 tttcttttat attaaatgga gaaaaaatac tttttgattt tgcctatatc accatacacg
      721 gaacccctgg tgaaaatggg attttacaag gatattttga tttaattgga ttaccctact
      781 cctgctgtgg agtattggct gctgctatta cattcaataa atttacgtgc aatcaatacc
      841 taaaaggatt tggcatacct gtagcccctt ctattttgct acgtaaaaac gaacacatac
      901 aaaacactgt ggttgtcgaa aaaatcggct taccttgttt catcaaatca aatgtaggtg
      961 gcagtagctt tggtgtaacc aaagtaaaag aagaaaagca aattcaacct gcaatcgaaa
     1021 aagcttttgc agaaggcaat gaggttatta ttgaaggatt cttacaagga acagaggtaa
     1081 cctgcggaat gtacaaaaca catgaaaaaa cacaggtatt tcctatcaca gaggtcgtta
     1141 gtgcaaacga atttttcgat tttgacgcca aatacaacgg tcaagtaaca gaaattactc
     1201 cagcccgtat ccctcaagaa gttacaaacc atatacaaca attaacaaat cagctatacg
     1261 atatacttgg atgcaaagga atcattcgag cagattttat tatcaacaaa caaaatgtcc
     1321 cttatttact cgaagtcaac actacacctg gaatgacccc tacaagcttt atacctcaac
     1381 aagttaaagc agctggtatg gctatttcat cggtaatgga agacattatt gaaaacaaat
     1441 taaatcacct ataatgtata tttaacagtc attcggcatg aaaagcagag aagaaattat
     1501 cgaaatagta caaaacgaac taactcttac caattggcta tcagaaccta tagacttata
     1561 taaacccata gcctatacga tgtctatggg aggaaaacat atacgctctg caatcacatt
     1621 gatggcttgc aacctataca gcgacaatat acatctggct cttcaacctg ccattggaat
     1681 tgaagttttt cataatttta cgcttttgca cgacgacatt atggataaag cagaaacccg
     1741 tagaggtaag ccaaccgtac atgtaaaatg gaacgataac accgctatct tgtcgggcga
     1801 tgccatgctc attaaagcct atcaactact ggcaaaatgc ccaccagaaa acctttctga
     1861 aatactttct gtattttctc aaacagctat tgaagtttgc gaaggacaac agtacgatat
     1921 gaatttcgaa catcaagata cggtttcaga agaagaatac attgaaatga ttcgtttaaa
     1981 aacagccgta ttattggctg caagcttaaa aattggtgct attatcggtg gtgcaactcc
     2041 actacaagcg aatgctttat acgattttgg tattcatatt ggcatcgcct ttcaactaca
     2101 agacgactta cttgatgtat atggagattc tacaattttc ggaaaaaaaa tcggcggaga
     2161 tattatctgc aataaaaaaa cttttttgtt gattcgtgca ctgaaaaatg caaacaatac
     2221 tcaacgtaat aaaatccaac actggatgac acaacatcct gatgacccaa ccgaaaaaat
     2281 tgtacaaatg acctcaatat tcaacgaaac gggtgtaaaa caagcctgcc aacaacgaat
     2341 gaaagagcat tataacaaag ccctacttgc tttgaataaa gcatgcgaag tagaagaaaa
     2401 aatagtacat ttacaaaaat tagcatcaga actaatgcaa agggaaaaat aatggaacac
     2461 gaatttggat tttacaaaaa tataacgtac atttgcgttt atcatatttt ttgttaacct
     2521 tatgccctac agaagacttc caaatacaga tgccgcacgt ttgagagcac tacgggctgt
     2581 tgcttgctac aaaaattcgc ctatcgacac cgaactgcct ttcgatagac gaatactaca
     2641 agaaatttgt agctttttgc ctcaatttga aaatgctatg tttgaataca aacaagccat
     2701 aaatagcgaa ggaaacaaaa acaacaaata ccaacaatac ataaaaaatg tgagattata
     2761 tatttcccat tttattcaag tcctcaattt agcgtgtatt cgtaacgaaa taaaacctga
     2821 aaaaaaatta ttgtataagc tattgcccga caattatgcc gtgcctgatt taagcacaga
     2881 aactttgctt ctagaatggg ggaaaaatat tatacaagga gaaaacgaac gaacacgtgc
     2941 tggaggttct cctatttaca atccgaccat cgcaaaagta caagtgcact atgatatttt
     3001 taaagaagca tatcactcaa aaaaagtagc acagaaaaat gtagggagat atgcagaaaa
     3061 tatgcagaaa atgagaatgc gagctgacga aatcatcctt gatgcttgga accaaattga
     3121 acaagctttt gttcattatg gtatggatgc gatgcaacaa aaagcaaaag aatttggaat
     3181 tgtctattac ttaagaagaa aagagagaca agcaatgaca aaaaaatcgc actaaaaaga
     3241 aaacttttgg cgtgtttatt tttttttgtg gaatatataa attaaatttg caccgtattt
     3301 tgtgagaatt actcgcggta atagcttttt tatgagcttt ttatttacta atacttactc
     3361 ggatgaagaa atttatactt ttactaacca tatttattct aagcagtttt gtagcattta
     3421 gtgccgaatt gccttccaaa ggatactctg ggtcatcctt ttcggatgga ggaagtttta
     3481 tggatgagcc aaccttaagt gtaggaaaca gaattggtgc tactaattca gtattaggag
     3541 tcaatgcatg tcagaaagct tgttcagata agctaatgat tgagtctgtt gcgtgtggag
     3601 gaaacattat ttgcctagaa gcttgtttta ttgcttatac tgcttgtatg gatgcttgcg
     3661 aaacatactc tcttcccatt gggtcaggat tcttcttttt gttactgctt agcggaggat
     3721 atgctggaac caaattgaac aagcttttgt tcattatggt atggatgaga tgcaacaaaa
     3781 agcaaaagaa tttggaattg tctattactt aagaagaaaa gagagacaag caatgacaaa
     3841 aaaatcgcac taaaaagaaa acttttggcg tgtttgtttt tttttgtgga atatataaat
     3901 taaatttgca ccgtattttg cgcgaattac tcgcagctta tagctttttt ataagctttt
     3961 tatttactaa tacttactcg gatgaagaaa tatatacttt tactaaccat atttattcta
     4021 agcagttttg tagcatttag tgccaaattg ccatcaaaag gatactctgg ggcatccttt
     4081 tcggatggag gaagttttat ggatgagcca accttaagtg ttggaaacag aattggtgct
     4141 actaattcag tattaggaga cggcgttgat tgtatagatg cttgtggtga gaccctagag
     4201 tttgcgattg ctgagtgtaa cggagacgat gtttgcgaag atatggcttt aactgctttt
     4261 ggaacctgcg tggatgcttg cgaaacatac tctttaccca ttgggtcagg attcttcttt
     4321 ttgttactgc ttagcggagg atacgctgga gtcaaattga gaagaaaaag aaaagaagca
     4381 taaaaaatta agcataataa caaaaataca aatagagtta gtgaaaactg actctatttt
     4441 ttttgttgct cgtttatagt tactcgagca gaactatcaa ctaaaagcac cgcacactaa
     4501 acactaaacg nnnnnnnnnn aaccaaaaaa aaaccgataa gtgaggggcg ttagccctga
     4561 tatcttagtt gatagttgac aattgacagt taaaagttat aaagttgaaa gtataaagta
     4621 aacccaacaa caacacccct cccaaaaaaa ctttctaact ttatgaactt tttaactttt
     4681 taactttcta actttatgaa ctttcaactc tccactttca aaaccacccc tctgttcttt
     4741 ctcccatcaa cttttacaag taatggagat gaaaatcgta catgacgtat atattctgtt
     4801 tcgtgcaaag cttcctgttc gtccaaataa gcacaatcat aatagtcatc ttcgttttga
     4861 gttatcgtaa aataacacac accaaaagac gttaaattct gaaaaaaatg agtaccttga
     4921 cttgggtcga tatgatacga tgtttctcca gcttcaacaa taacgcgtgc tcccgaaata
     4981 tgtggccact taataggaat acctaaccaa tggtcactac ttccccagcg accaggacca
     5041 accaatatat aagatttatc ttcagcaact aattgcttgt ttattttttc gatatcataa
     5101 gctatcaaga tatttttaga aggattgaaa gctccttttt taacgtatac tacatcatag
     5161 acatcattca taatgccatt tcctaaagca ttagtagtcg agagaaaagt tttttctttt
     5221 gaaataaggc ttatatcttc tgttaacgat tcatttaagc ctacaattgg acgtatttga
     5281 agtaaataaa aggtgtgttg ctcatttttt tcataatcta aatgtacggc aaattcaatt
     5341 tctactggac atcccatttc tttttcgcca accgtcaata catttttcaa tatatccgcc
     5401 aattgaaaag actgatgttc taagatattt acaaaagtga ttaacttgcg tccacctgca
     5461 tatactccat cacgtattac ttggtcaata ggatcaaaag tagatgctaa ccaacgtaat
     5521 gtaccatctt gttcagcctc tctaatatgt agtttttgca aattatcgcc atcatctact
     5581 cttggtttaa acgacctttg tgctaaatcc aaacaataaa aataattttg cgtttcacta
     5641 agagcaaact ccatggtact cgtttgaagc accttatttg gataagctgg acaaaaacgt
     5701 aatgtttgcc ctccatcaac aatgtatttc cctaaaccta atgcaacatt aacaacacct
     5761 tcttcgggtt tttcttcgcc aataggataa taatttaacg atcgtgccac tcccgaaaaa
     5821 gaaggataaa accgatttcc ataagcattt ccacacactt cttgtaacac tacagccatt
     5881 ttctcttcat ctatcaggtt tttagtggct ttcatgtatg ctttgctatc tgcataaaat
     5941 acagatgcat ataccgcttt aatagcatcg cacaccatcc gtaaagtagt agtacgactc
     6001 tctgatacat tcggaaccat ataggtggag taaattcccg caaaaggctg ataatgagaa
     6061 tcttctagta agctagacga tcgaactgca atcggacttt gaattacccc taaaaaagca
     6121 tacaaatctt tcatgatttt tcgtggcaat cttgcctcca aaaaaacctc taaaatttgt
     6181 tcatcggata catcttctcg caacgctact acatacaaat tgtttgtttc cataaactca
     6241 tcaaacatat ctgtacaaag cacaactgtt ttaggaacag ttatttgcgt attttcaaaa
     6301 tcatacaaag ttggattacg ttttgaaaca gcattcaaaa atgctaaccc tctccctttt
     6361 cctcccatcg aatcttctcc tattcgtgca aaattggagt attcatcaaa tctatcgctt
     6421 tgaaaaacag ccacaatacc tttgttttta atcttgcgat aatttacaat cgcatcaaac
     6481 acaatatgtc ttgccctgtc aatatctttt atgtctttaa taatgatata acgcaaatat
     6541 tcagctagcg gaaacatcgc ccgtgaatag agccatcgag ataaatcatt ttgagccaaa
     6601 tgataaaaca aagaatttgc aggaatatca aaaatatgtt tctgcaaatc ttgcaaatta
     6661 tgtaccgtta tcaaagcttc acgggtgtca ggagacacaa ataccaaatc gccaaactta
     6721 aaatgatgcg ttatagcgtc tttaaattgt ttgtgaaagg ttttgtctgt tttatctaaa
     6781 aaaacagcac atagcctgtc tgtttgctca caaacgccct ttggctcact tgactcaacg
     6841 ataataggaa caaacggatc caactttcta atttcctgac ttaactccaa tccagcggtt
     6901 ggatgtatct gacccgctct aggaaaacgc acatcagtta ttacacccaa catgtttttt
     6961 tgatattttt tatataaaaa catagcttct tcgtaagtgc gagctaataa aatcttaggt
     7021 cttcctcgca tccgtaacat ttgctggtga gcattcagtg cttcggtcat aaatgacagc
     7081 gattgagtaa atacatgttt gtacaaatat ggcaaaatag aagaataaaa atgaatggaa
     7141 tcttcaacaa acaaaataac ttgtacacct actgaggtaa catcatcatc tacatttaac
     7201 ttatcttcta gcaatttgac aatagccaat aataaatcag aatttcctag ccaactaaat
     7261 acatagtcaa catgtaaaaa atcgccctca gaaaataccc gtttaaagat gcgacgtgat
     7321 aaggttgtta aaacaacaat tggaatatct gcgtaactaa ccttcaattc atttgccatt
     7381 tccaagatat gactattatc acttgttggc atcgcaataa ttaggtcaaa atgacgcgtt
     7441 tgtaataatt cttttacttc tttgtgttca ctggcaatag taaaacgtgg tggataacgc
     7501 aaattaagtg ctgtatattc gttgaaaatt agttcatcaa tcctgccatc ttcttcgagc
     7561 ataaaagcat catacttgct cgcaataagt agtatattat aaatacgttc tttcattaaa
     7621 ctaacaaaag aagtatcttt aaaatagagt ttctgtagat ttaaggtttt gtccatctaa
     7681 tagttttttg atgatgctaa ggtaattaaa aaatttgaga aagttgaatg attagtggtt
     7741 aatgtttaat gtttagtgtt tagtgtttag tgttgaatgt ttaatgttta gcgttgctcg
     7801 ttgagcgtta ctcgtttttc gagcttaact atcccctgaa aaaaggagcg tagctctgct
     7861 atcttcgtag caaaatacaa tacgtcatat tattaggggc gttcgccctg aaatcttggt
     7921 tgatagttga taattgacag ttgaaagtgt ttagtgttga atgttgaatg ttttgcgttg
     7981 ctcgttttgc gttgctcgag cagaactttc aactgtccac ttccactaaa tacgcgacct
     8041 cgtttatgta gccaaaaaat gcagtgaaat gagcggttaa aaaagaatgc tgtttgagat
     8101 ccgatttatc ggagcgagtt tttattgagt tgatagtt
//
LOCUS       DAOSRP010000090         8130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8358_length_8130_cov_4.779690, whole genome shotgun sequence.
ACCESSION   DAOSRP010000090 DAOSRP010000000
VERSION     DAOSRP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8358_length_8130_cov_4.779690"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /gene="ovoA"
                     /locus_tag="PLJ82_06455"
     CDS             <1..1648
                     /gene="ovoA"
                     /locus_tag="PLJ82_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HOR41182.1"
                     /translation="IEHERIHIETSSVLIRQLPLEEVVSGRFGNTCTLSGTAPRNEMI
                     PVKGDTVVMGKPSNHNLYGWDNEYGQHKELVADFSASKYLVSNSEYLGFIEENGYNTA
                     KYWTEEGWKWRTYTQAEMPLFWRKKGNKFWLRLVAEEIEMPWNWPVEVNYLEAKAFCN
                     WKTQKEGKTYRLPTEAEYVQLTRVCAVPDELEWETAPGNINLEHFTSACPVDMFALGE
                     FYDVIGNVWQWTETPITGYAGFKVHPMYDDFSTPTFDGKHNLIKGGSFISTGNEATHH
                     ARYAFRRHFYQHAGVRLVQSEMKLVIQHAEYETDREIANECESQWNTEDARNFDVQLA
                     AYIKEQISHLDTKKVLDINTNTGRLPFLLAQSVAKITAIDFSARFIRMPIQLQENGFM
                     RYIVTDENELVHYREIVIDSHLQEIAKEKIVFLQDNANNLKPIHSGFDVIIANNVLEE
                     LNNPIQFLQTIHQRLHKQGILIVASSYNWKKNNIPAENWLGGYKKDGEPVTGLDAIKE
                     NLQNTFVLLDKPFDLQKIAYISTRESSVQRCEVSIWQKKS"
     gene            1769..1948
                     /locus_tag="PLJ82_06460"
     CDS             1769..1948
                     /locus_tag="PLJ82_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOR41183.1"
                     /translation="MEEDIIICHCNEIYKSEIVKAIKEKGLTTVDEVGDETSAGTICG
                     QCQDDIQDILDEINK"
     gene            complement(2156..2229)
                     /locus_tag="PLJ82_06465"
     tRNA            complement(2156..2229)
                     /locus_tag="PLJ82_06465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2193..2195),aa:Asp,seq:gtc)
     gene            complement(2321..2932)
                     /locus_tag="PLJ82_06470"
     CDS             complement(2321..2932)
                     /locus_tag="PLJ82_06470"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOR41184.1"
                     /translation="MPILDNIHTPSIDVAIWHITETADELFSMLLPNPVYTNELNRIS
                     SNKRKSEFLAVRLLMQHLVGDSTIAYLSNGKPILSGSSRFISISHTKEYVAVAISNQH
                     EVGIDIETISAKVHQVKERFMNETELVHAQQYPEFVLLSWVAKEAAYKKIGDGVVHFS
                     QSMELVPFVPHLSGVLELKVQLLEETRYSFYYQQHTDFVLVYG"
     gene            complement(2946..3512)
                     /gene="gldD"
                     /locus_tag="PLJ82_06475"
     CDS             complement(2946..3512)
                     /gene="gldD"
                     /locus_tag="PLJ82_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOR41185.1"
                     /translation="MNCNIKHILAVGCSIVMVACTNYTPKPMGYFRIALMEKEYAYTQ
                     ANMPYTFEYPTNVARIRPHKNNPLWMNIEYPTYNAKIHCSYLPIKDNFYEISEDTRAF
                     VYKHSVKADAITQQVFENDEAKVYGVLYEIKGNVASSLQFTLTDSTTHYFRGAVYFNN
                     RPNNDSIAPVLSYIREDVVRMMETMQWK"
     gene            complement(3502..4812)
                     /gene="gldE"
                     /locus_tag="PLJ82_06480"
     CDS             complement(3502..4812)
                     /gene="gldE"
                     /locus_tag="PLJ82_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOR41186.1"
                     /translation="MEQLYVNSTYVSLFFSILCCLVLLLCSAFVSASEVAFFSLLPKD
                     IEELTASSDRKDTCILELKSNSDRLLATILLANNLVNIAIIVLSTSILFSLFDFSDSI
                     WLGFLVETVFITLILLLFGENMPKIYAAKYPLRFARFAAPTMRVLIKLLHPFSWLLVK
                     STSNVKLFDKKKSIKTLSTSDLSKAYELTSSELEEDKEILEGIIRFSNTEVESIIVPR
                     VDVVAVDITDSFTDIIRIINESGYSRLPVYADTIDNIKGVLFIKDMLAHINKGDEFKW
                     QKHIRPAFFVPTTKHINVLLEDFQKNKTHIAVVVDEFGGTVGIVTLEDILEEIVGDIS
                     DEYDDENERSYIQLDTNTFVFEAKILLEDFYKIEGIEKKDFEKIAGEPESLAGLILEI
                     KGEIPQVGDVVLYNNYEFEIVSADKRRIKTIKLTIHAPIQEDEL"
     gene            5029..7494
                     /gene="pheT"
                     /locus_tag="PLJ82_06485"
     CDS             5029..7494
                     /gene="pheT"
                     /locus_tag="PLJ82_06485"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOR41187.1"
                     /translation="MNISYNWLKQYININLPVHEVSHLLTSLGLEVGSVEEVETIKGS
                     LEGLVVGEVIESSKHPDADRLHVAQVRIQPTEEPIQIVCGAPNCRAGIKVVVATVGTK
                     LYSGEEVFTIKRSKIRGVESNGMLCAEDEIGIGTSHDGIIEIASNIEVGTPVKELYNV
                     DTDTIFEVDITPNRVDAASHIGVARDLAAALRLKDSTIRLQKPSVESFVVDNKEYTIN
                     VEVTNPTLCPRYCGVTINNIQVKESPEWLKNRLLMIGMRPINNVVDITNFVLHETGQP
                     LHAFDGDKITGNKVRVTTLANNTPFVTLDEVERKLSNEDLMICNANEGMCIAGIFGGL
                     DSGVSNTTTKLFLESAYFNPVSIRKTARRHGLNTDSSFRFERGCDPNNTLYALKRAAL
                     LIKEVAGGEISSDIIDIYPSPIQATEVKLTYAKINSLIGKVIDKEIIRTILTGLEIDI
                     VNENNDVLTLSVPAYRVDVQRDVDVIEDILRVYGYNNIIPSDELKSSITYSQKPDSHK
                     LQNTLAEQLTGAGFNEILNNSLTKIAYYKELNSFPENEAVKILNPLSADLSVLRQTLL
                     FGGLESITYNSNRRNPNLKFYEFGNCYHYDDAKKNNEEPLKAYSEELHLGLWITGNKT
                     KQSWTDTERKVSFYDLKGYVETILQRLGIKNESISVSDYSNDLLPNALEIVSKNKMPL
                     VVIGKVSNKVLKQFDIDTDVFYADFNWENVLKVIVKQKVRYQEIPKFQEVKRDLALVL
                     DKHIRFQDIEQIAFATEKNLLKKVTLFDVYEGKNIEQGKKSYAISFVLQHDQKTLEDS
                     QIETIMKKILQALETQTGAKLRQ"
     gene            7522..>8130
                     /locus_tag="PLJ82_06490"
     CDS             7522..>8130
                     /locus_tag="PLJ82_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR41188.1"
                     /translation="MGRAFEYRKATKLKRWGNMARVFTKLGKQITIAVKEGGPDPDTN
                     PRLRVLAQQAKKENMPKENVERAIKKASSKDEADYKEMVYEGYGPNGIAIVVETATDN
                     PTRTVANIRSYFNKFGGSLGTSGSLQFLFDHKCVFKIAPKPTVSLEDLELELIDFGVD
                     ELLEDEGEIILYGEFQSYSSIQKYLEENGFEIHSAEFERIPND"
BASE COUNT         2841 a         1409 c         1487 g         2393 t
ORIGIN      
        1 cattgaacac gaacgcattc acatcgaaac ctcatcggta cttattcgtc aattaccttt
       61 ggaagaggtg gttagtggac gatttggtaa cacctgcacc ttatctggca cagcaccaag
      121 aaatgaaatg atacctgtaa aaggcgatac tgtagtaatg ggcaaaccaa gcaaccataa
      181 cctttacggt tgggacaatg aatacggaca acacaaagaa ctagtagctg attttagtgc
      241 tagcaaatac ttggtgtcaa attccgaata ccttggtttt attgaagaaa atgggtacaa
      301 tacagcaaaa tactggacag aagagggttg gaaatggcga acatatacac aagcagaaat
      361 gcccttgttt tggagaaaga aaggcaacaa gttttggcta cgtttggtag ccgaagaaat
      421 tgaaatgcct tggaactggc ccgtagaagt taattacctc gaagccaaag ctttttgcaa
      481 ctggaaaaca cagaaagaag ggaaaactta tcgcttgcct actgaagccg aatatgtgca
      541 actaacacgc gtatgtgctg tacccgacga attggaatgg gaaacagctc caggtaatat
      601 caatttagag cattttactt cagcttgtcc tgttgatatg tttgccttag gtgagtttta
      661 cgatgtaatt ggcaatgtgt ggcaatggac tgaaacgcct attacgggct atgcagggtt
      721 taaggtgcac cctatgtacg acgatttttc tactccaact ttcgatggga aacataattt
      781 aatcaaaggt ggttcgttta tatctactgg taacgaagct actcaccatg ctcgctatgc
      841 gtttagacgc catttttacc agcatgctgg cgtgcgtttg gtacaatctg aaatgaaatt
      901 agtgattcaa catgctgaat acgaaacaga tagagaaatt gcaaatgagt gcgaatcgca
      961 atggaatacc gaagatgcaa gaaactttga tgtacaatta gccgcttata tcaaagaaca
     1021 aattagccat ttagatacta aaaaagtgct tgatatcaac accaatacgg gcagacttcc
     1081 gtttttgttg gcacaatctg tggcaaaaat tactgccatt gatttttcgg cacggtttat
     1141 acgtatgccc atacagttgc aagaaaacgg ctttatgcgg tacattgtaa cagacgaaaa
     1201 cgagctggtg cactaccgcg aaattgtaat tgacagccat ttgcaagaaa ttgccaaaga
     1261 aaaaattgtg tttttgcaag acaatgccaa caaccttaaa cctattcaca gtggatttga
     1321 tgtaataatt gccaacaatg tattggaaga attgaataac cctatccagt ttttgcaaac
     1381 cattcatcaa cgattacata agcaaggcat attgatagtg gcttcaagct ataattggaa
     1441 aaaaaataac atacctgcag aaaattggtt gggcggctac aaaaaagatg gcgaacctgt
     1501 aacaggactg gatgccataa aagaaaattt gcaaaatacc tttgttctgc ttgataaacc
     1561 ctttgatttg caaaaaattg cgtatatctc tacaagagaa tcaagcgttc aacgatgtga
     1621 agtaagtata tggcagaaaa aaagctagta agttttataa acagtatata aagtaaatta
     1681 actaaaataa ttgcgatttt taatcacttt gctatacctt tgtttttaga gtgaatgaaa
     1741 gaaattacta atttaactgg aaaccattat ggaagaagat ataatcattt gccattgtaa
     1801 tgagatttac aaaagcgaaa tagtaaaagc gattaaagaa aaaggcttaa ctaccgtaga
     1861 tgaagtgggc gacgaaacct cagcaggcac tatttgcggt cagtgccaag acgatatcca
     1921 agatattttg gatgaaataa acaagtaaaa aaactattct actaaaaata gccatcaagt
     1981 ttgatggcta ttttggttta taaaagttta tgttttcaat attgacattt ttggagaggt
     2041 atttttggat gagacagtcc aattgagtat tctatatttt cagcaccact gcacggcaaa
     2101 tgcgacacat aaaaacaaaa tagcctgata atcagatgat taacaggcta tttagcggta
     2161 tggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccagctgaac
     2221 taccacacca tgtgtttcgc tcaattgcga tgcaaagata tgctgatttt ttatattata
     2281 caagtgttga tggtattttt ttgataaaat cgacaaaaaa ttacccatat accaatacaa
     2341 agtcagtatg ttgctgataa taaaaggaat accgtgtttc ttcaagtaat tgtaccttta
     2401 actccaacac gcctgataaa tgcggaacaa acggcacaag ctccatagac tgggaaaaat
     2461 gcaccacccc gtcgcctatc tttttgtatg cagcctcttt agctacccaa gataacaaga
     2521 caaattcggg gtactgttga gcatgtacaa gctctgtttc gttcataaaa cgttctttta
     2581 cctgatgtac cttggcagaa attgtttcaa tatcaatgcc tacttcatgt tgattgctta
     2641 tggcaactgc aacatactct tttgtatgcg aaatactaat aaaacgcgac gaaccagata
     2701 aaataggttt accattcgat aaataggcaa tagtgctatc ccctactaga tgttgcatta
     2761 ataaacgcac tgccaaaaat tcggactttc gtttattgct agatatcctg ttaagttcgt
     2821 ttgtatacac aggattgggc aatagcatcg aaaacagctc gtcagcagtt tcggttatat
     2881 gccaaatagc tacatctatg ctaggtgtgt gtatgttatc aagaataggc actttggtcg
     2941 aaatattatt tccattgcat ggtttccatc atgcgtacta catcttcgcg aatgtaagac
     3001 aaaacgggag caatagaatc gttattgggt cggttgttaa aatagacagc acctctaaaa
     3061 taatgggtag tactatcagt cagagtaaac tgcaaggaag aggctacatt gcccttaatt
     3121 tcgtacaaca ccccatatac ctttgcttcg tcattttcaa aaacttgctg ggtaatggca
     3181 tctgctttta ctgaatgttt gtagacaaaa gctctggtat cttcggatat ttcataaaaa
     3241 ttatccttta ttggcaaata actacaatga atttttgcat tatatgtagg atattctatg
     3301 ttcatccaca atgggttatt tttatgtgga cgaatgcgtg caacattggt aggatattca
     3361 aacgtatagg gcatatttgc ttgtgtatat gcatattcct tttccattag tgctatccga
     3421 aaatagccca ttggtttggg agtgtagttg gtacaagcta ccattacgat actacatcct
     3481 actgctagaa tatgttttat attacaattc atcttcttga ataggtgcat gtatggttaa
     3541 tttaatcgtt ttaatgcgtc ttttgtctgc cgatacaatt tcaaattcgt agttattata
     3601 caaaacaaca tctcctactt gcggtatctc cccttttatt tctaagatta aacctgccaa
     3661 actttctggt tcacctgcta ttttttcaaa atcctttttt tctatacctt caattttgta
     3721 aaagtcctcg agtagaatct ttgcttcaaa cacaaatgta tttgtatcta gttgaatata
     3781 agagcgttca ttttcatcat catattcatc actaatatcg cctactattt cttctaaaat
     3841 atcttcgagg gtaacaatac ctacagtacc accaaattca tctacaacta cggcaatatg
     3901 tgttttattt ttctgaaaat cttcgagtaa tacattgatg tgttttgtag ttggaacaaa
     3961 aaatgcaggt cttatgtgtt tttgccattt aaactcatct cccttgttga tatgagccaa
     4021 catatctttg ataaacaaaa cacccttaat attgtcaata gtgtctgcat aaacaggtaa
     4081 acgcgagtaa cctgactcat taattattcg aataatatca gtaaacgaat ctgttatatc
     4141 cactgctact acatctaccc ttggcacaat aatactctct acttctgtat tactaaaacg
     4201 tataattcct tctagaattt ctttatcttc ttctaattcg gatgaagtaa gttcgtatgc
     4261 tttagataaa tcactggttg acaatgtttt gattgatttt tttttatcaa ataacttcac
     4321 attgctggta gatttgacca acagccatga aaaagggtgt aatagtttaa ttaatacacg
     4381 catagtgggt gcggcaaatc gtgcaaaacg taatggatat tttgccgcat aaattttggg
     4441 catattctcc ccaaataaca atagaataag tgtaatgaat actgtttcga ctaagaaacc
     4501 taaccatatg gaatccgaaa aatcaaacaa cgaaaaaaga atactagtag acaatactat
     4561 aatagcaata tttaccaaat tattagcaag caaaatagtt gccaataagc gatctgaatt
     4621 gctttttaat tccaaaatac acgtatcttt tcggtcgcta ctagctgtta attcttctat
     4681 atcctttggt aatagagaga aaaatgctac ctcagaagca gatacaaaag ccgaacacaa
     4741 caataaaact aagcaacata aaatcgagaa aaacaaggaa acgtatgtac tattaacata
     4801 caattgctcc ataaaaagac gtatcgattc aaaaatatcc aatgtattac aatttaaata
     4861 aaacactacg taattagtaa aacggaaaaa tacgaagaca aagatataaa cttttaaaga
     4921 aaatagtgaa atgaaatgat gaagaatgaa ttttttaagg gaataatttt ttgtaatagc
     4981 agatgtttgt taaatttgca ctcttaaaat tattttaata caaaaaaaat gaatatttct
     5041 tacaattggc tcaaacaata catcaacatc aatcttcctg tacatgaagt atctcaccta
     5101 cttacttctc ttggtttaga agtaggctca gtggaagaag tggaaacaat caaaggaagt
     5161 ttagagggat tggtagtagg tgaagtgatt gagagtagca agcatcctga tgccgaccgt
     5221 ttgcatgtag ctcaagtacg tattcaacct actgaagaac caatacaaat tgtatgtgga
     5281 gctcctaatt gtcgtgctgg cataaaagta gtagtagcta cagtaggtac gaaactgtat
     5341 tctggagaag aagtctttac cattaaacgc tctaaaatac gtggagtaga atcaaatggt
     5401 atgttgtgtg ccgaagacga aattggcatt ggcactagcc acgatggcat tattgaaata
     5461 gcctcaaata ttgaagttgg aacgcctgtt aaggaacttt acaatgttga cactgacact
     5521 atttttgaag tagatattac tcccaaccgt gtagatgcag catctcatat tggtgtagca
     5581 cgcgatttgg ctgctgcttt acgattaaaa gactctacta tacggttgca aaaaccctct
     5641 gtagaaagtt ttgttgttga taataaggag tataccatca acgtagaggt aacaaaccct
     5701 accctttgtc cacgctattg tggggtaacc attaataata ttcaagtaaa agaatcgccc
     5761 gaatggctca aaaataggct actaatgatt ggtatgcgac ccataaacaa tgtagtggat
     5821 attacaaact ttgtgttgca cgaaacagga caacctttgc acgcatttga cggagataaa
     5881 attactggaa ataaagttag ggttactacc ttagcaaata acaccccttt tgttaccctc
     5941 gatgaggtag aacgaaaatt atccaacgaa gatttgatga tttgcaacgc caacgaaggc
     6001 atgtgtatag caggtatttt cggaggatta gactctggag taagcaatac tacaactaaa
     6061 ttatttttag aaagtgctta ttttaatcca gtaagtatac gcaaaacagc tcgtagacat
     6121 ggactcaaca cagactcttc ttttcgtttt gaaagaggtt gcgacccaaa caacacctta
     6181 tatgcactaa aacgtgccgc attactcatt aaagaagtag ctggtggcga aatttcttct
     6241 gatattattg atatatatcc ctctcctatt caagcgacag aagtaaagct tacatacgcc
     6301 aaaattaatt cattaatagg aaaagtaatt gacaaagaaa taattagaac aatattaact
     6361 ggcttagaaa tagacatagt taacgagaat aatgatgtac ttacgctgtc tgttcctgcc
     6421 tacagagtag atgtacaacg tgatgtagat gtgattgaag atattttgcg tgtatatggt
     6481 tacaacaata ttattccgtc tgacgaatta aaatcatcca ttacgtattc tcaaaaacct
     6541 gattcacata aattacaaaa cacactagca gaacagctta caggagctgg ttttaacgag
     6601 attttaaata attcgcttac aaaaattgct tattacaaag aactaaactc ttttccagaa
     6661 aatgaagcag tgaaaatact aaatccttta agtgctgatt taagtgtttt gcgacaaacc
     6721 ttgctgtttg gtggtttaga aagcattacg tataacagta acagaagaaa tcctaatttg
     6781 aaattttacg aatttggaaa ctgctaccac tacgatgatg ctaaaaaaaa taacgaagaa
     6841 cccctaaaag catattcaga agaattgcat ttgggcttat ggataacagg aaacaaaacg
     6901 aaacaaagtt ggacagatac tgaacgtaaa gtgagttttt atgatttaaa agggtatgta
     6961 gaaacaatat tgcaacgatt aggcattaaa aatgaatcta ttagcgtttc tgattacagc
     7021 aatgacctat tgcctaatgc tctcgaaatt gtaagtaaaa ataaaatgcc attggtggta
     7081 atcggaaagg tttctaataa agtgttaaaa caatttgaca ttgacacaga tgtcttttat
     7141 gcagacttta attgggaaaa tgttctgaaa gttattgtaa aacaaaaagt tcgttaccaa
     7201 gaaataccta agtttcaaga agtgaaacga gaccttgcat tagtactcga caaacatatt
     7261 cgttttcaag atattgagca aatagcattt gctacagaga agaatttgtt gaagaaagtg
     7321 actttgtttg atgtatatga aggaaaaaac attgaacaag ggaaaaaatc atatgccatc
     7381 agttttgtgt tacaacacga ccaaaaaaca ttagaagatt ctcaaataga aactattatg
     7441 aaaaaaattc ttcaagcctt agaaacacaa actggagcaa aattacgtca ataattaacc
     7501 ttatataaca aattacaact catgggaaga gcatttgaat atagaaaagc tacaaagctg
     7561 aaacgttggg gcaatatggc tcgcgttttc acaaaattag gtaaacaaat tacgattgca
     7621 gtaaaagaag gcggtcctga cccagataca aatccacgtc tgcgtgtgct agcccaacaa
     7681 gctaaaaaag aaaatatgcc caaagaaaac gtagaaaggg ctataaaaaa agcatcttcc
     7741 aaggatgaag ctgattacaa agaaatggta tatgaaggct atggaccaaa tggtatagct
     7801 attgtggtag aaacagcaac agacaaccca actcgcaccg ttgcgaacat acgtagttac
     7861 ttcaataaat ttggaggttc gctaggaact agtgggtctt tacaattttt atttgatcat
     7921 aaatgtgtgt ttaaaattgc tccaaaacca acagtctcat tagaagattt ggaattagaa
     7981 ttaatagatt ttggtgtaga tgagctacta gaagacgaag gagaaattat tttatacggc
     8041 gaatttcaat cgtattctag catacaaaaa tacctcgaag aaaatggatt tgaaattcac
     8101 agtgctgaat ttgaacgcat acccaacgat
//
LOCUS       DAOSRP010000091         8060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8438_length_8060_cov_5.108557, whole genome shotgun sequence.
ACCESSION   DAOSRP010000091 DAOSRP010000000
VERSION     DAOSRP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8438_length_8060_cov_5.108557"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1283
                     /locus_tag="PLJ82_06495"
     CDS             123..1283
                     /locus_tag="PLJ82_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOR41189.1"
                     /translation="MNKKNYSIPIAILSVLIVAFFTACRTDEPVLLPSKTPVTDETTN
                     SEVVGFYLLNEGNMGSNKATLDYFDFTTGIYTKNMYAATNPQVVSELGDVGNDLQIYG
                     NKLYAVLNTSGFVEVMDATTAKHIGMIEIPNCRYITFYNGKAYVSSYAGPVELSNNTQ
                     VGYVAEIDTASLQITRTVHVGYQPEEMAIVNGTLYVANSGGYMYPEYDNTVSVICLQT
                     FTETQKVEVGINLHRLKSDNKGNLYISSRGNYTDIASCLYILNTNTLQVDKKVDIPVS
                     NLCIVGDSAYVISSEFNFLSGNTNIEYALINTSTQNIEANNFITDGTETAIKMPYGIA
                     VHPITRDIYITDAKSYIIPGSVYCYSKTGKRKWYVTTGDIPAHIAFLTKQNN"
     gene            1300..2427
                     /locus_tag="PLJ82_06500"
     CDS             1300..2427
                     /locus_tag="PLJ82_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41190.1"
                     /translation="MKRNLLVTILLGLIVCNLQAANSAYVTEIIDYRPAPGQHINRSF
                     PPADKSDSHENAVAWAATQLVGKTNGLVGLGAFGGYIIVGFDHSIVNVAEEYDFKVYG
                     NAFANGAEPGIVMVCQDLNGNGVPDNDEPWYELAGSDYQLSSTIKNYEITYTRPNPDL
                     QKSHIQWTDNQGGSGQVTHISFASQSTMYPLWIADNTLTFRGTKLAGNAIQNGSMFTL
                     PALSWGYADNQPNNSTNDKTSFNIDWAVDASGNPVQLSHIDFIKIYTAMVQEAGWLGE
                     TSTEIAGVEDLHPNASLPTVIHSQENKQMYIQNPFNETLIVKANENCEMILYTISGNL
                     IGKYMITPGENTITTSQLPTGNYIVHIINTKQNHIYKLIKH"
     gene            2431..3465
                     /locus_tag="PLJ82_06505"
     CDS             2431..3465
                     /locus_tag="PLJ82_06505"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOR41191.1"
                     /translation="MKTTLLTLISLLLLASNTFATNIVLNLSNPTTPANINYDTEKKY
                     WTDTYSESSDYTWLQFQNFKLAHLIDQDSWGGTYWDGFTISTSGDNTDYSSTGWMGNE
                     WGCMAGGGIKTDQSGAIVTTNGVVDTEQGIPYLVAYYSAYASNGYSNEIKFADGQKYE
                     AVEVYVTNHPWPYYENMNGGMFTRVLDQEGDYVKLIANGLDENGNSTGSASFYLAKFE
                     NGSLTQVTNWEKFDLSALGTIASLYFTIESTDTGDWGMNTSTFFCMDKLTVKEYTTSI
                     PQLATENAAYRVNNILYNIPENATITVYNLQGQLVYSTMATSNTVSLPIVNNHLIIKI
                     VNENNTQIIR"
     gene            3566..6061
                     /locus_tag="PLJ82_06510"
     CDS             3566..6061
                     /locus_tag="PLJ82_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41192.1"
                     /translation="MEIFFISNLFSTFVLSKKKNHSAQFTMNIYTLKTILIATLLAAI
                     GFSGFAQAEQGGALRGRVYNPDNNQPVDFAYVIIDGTTIGTITDLDGNFAIFGLKPGY
                     VRVAVNSLGFDPVVTKEILITNANTPYIEIEMKPSNIQLDDVVVEASRFRQREESPLS
                     LRSIGIKEIEKSPGGNRDISKVIQSLPGVGATPANRNDIIVRGGGANENRFFLDGVEI
                     PNINHFATQGSSGGPAGIINVDFVRELDFFAGAFPANRGNALSSVLDFRLIDGNKDRL
                     KTRATVGASDLALTLDGPIGDKSSFVFSVRRSYLEFLFDLIGLPFLPTYNDIQFKHKI
                     RFNQNNELSFIGLGAFDVNRLNLSANETESQRFILKYLPTNDQWTYTVGAVYRNFFKN
                     GNQTLVLSRNHLNNVSQKYQDNIEAPTNLNFDYASNEIETKIRYEYVTRQGAWKFLYG
                     AGAEYNQYTNYTYNKLFFNAQVVEFDYETELDFYSYSAFTQISRNLLAERLLVSAGAR
                     IDGNTYSEQMSNPLEQFSPRISASYALTNKWSLNANTGRYYQRPAYTTMGYRDIAGNL
                     INKDNRLRYKYTDHFVVGVENKPNPNSRFTVEGFYKLYNDYPFSISDSVAISSKSADF
                     DVFGAEAVLSIGEGRTYGVEILYRNTDLWGSNITTSFTLFRSEFKDSKEVYIPTAWDN
                     RILFNLTATRPLPKNWDIGFKWRYVGGAPYTPVDLERSSLRQAYDVTGRTYLDYDRFN
                     SERLAPSHQLDVRVDKAFFFNKWSLMLYLDIQNIYNFKSQQPDQYINEDKDGNVVIEN
                     PTAPLNEQRYVLRKLENTGSGTVLPSIGIMIEF"
     gene            6103..6807
                     /locus_tag="PLJ82_06515"
     CDS             6103..6807
                     /locus_tag="PLJ82_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41193.1"
                     /translation="MTTKKYILLIASLLLSTAYVAANKQTLSTIELSVEKGKSYNYPT
                     FALWLESAGGKFVQELYVTQSLATGIYNNADAGDKRWKREKGEARRPSTLPYFLHKRD
                     IQAPDGTYLPTPEQPIPDAYTGATPRQSDVITIVLDRQLYGKYRILFEINQPWDFNRV
                     WYNNRYPNNTDYLLSGQPSLVYMVEIDTNNLQEEYTLNPIGHGHPYGKTGTLFTDISG
                     ITTAKNIFKSIKVKVY"
     gene            complement(6868..7077)
                     /locus_tag="PLJ82_06520"
     CDS             complement(6868..7077)
                     /locus_tag="PLJ82_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41194.1"
                     /translation="MKKLLCPQCRIHRFRVFNAARESVVVTVNEHYEVIPIHEGVSLE
                     GYFTDELHCLGCSWIGSPKALLNRY"
     gene            7193..7843
                     /locus_tag="PLJ82_06525"
     CDS             7193..7843
                     /locus_tag="PLJ82_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOR41195.1"
                     /translation="MKTALIIGSTGLVGSALLNELLAHPAYSKVISFTRKPSGNIHPK
                     LHEHCIDFNNIASYKDLIQGDDLYCAMGTTIKKAGSQAAFTLVDYTYPYQFATIAKSN
                     GVKQFLLVSSIGANAQSKNFYLQTKGTIENAISLLGFETVVIFRPSLLLGKRTEFRFG
                     EKIAMHISQLFSFLFVGKLRPYAPIHSHTVAKAMVSVAQQANTGVNIYASDAIKEV"
BASE COUNT         2681 a         1617 c         1494 g         2268 t
ORIGIN      
        1 ttggttatca atggactgct ttagtggaga taaataacct actaaaccaa caatatgatg
       61 tggtactaaa ctacccaatg ccgggaacga attttaaatg tagcttaaaa atacgtatat
      121 aaatgaacaa aaaaaattat tccataccaa tagctattct ctctgtgcta atagtggcat
      181 tttttacagc ttgtcgcaca gatgagcctg tacttttgcc aagtaaaacg cctgtaaccg
      241 acgaaacaac taatagcgag gtagtgggtt tttatttgtt aaacgaaggg aatatgggga
      301 gtaacaaggc tacgctcgac tatttcgact tcacaacagg catatatact aaaaatatgt
      361 atgcggctac caaccctcag gtagtatcag aattgggtga tgttggcaat gacttacaaa
      421 tttatggaaa caaattatac gctgttctca acacctctgg atttgtagaa gtaatggatg
      481 ccactactgc caaacatata ggcatgatag aaatacccaa ttgccgttat atcacatttt
      541 ataatggaaa agcttatgta agttcgtatg ctggacctgt tgagttaagt aacaatacgc
      601 aagtaggtta tgtagctgaa attgatacag caagtttgca aataacccga acagtacatg
      661 ttggttacca acccgaagaa atggctatag ttaatggtac gctgtatgtt gctaattctg
      721 gcggatatat gtatccagag tatgataata cggtttctgt aatttgccta caaactttta
      781 ccgaaacaca aaaagttgaa gtaggcatta atcttcatcg attaaaatca gacaataaag
      841 gaaacctata tatcagtagt agaggtaact acaccgacat tgcttcgtgc ttgtatatcc
      901 ttaacaccaa caccttgcag gtagataaaa aggttgatat tcctgtttca aacctatgta
      961 ttgtgggcga ttcggcttat gttatcagtt cagaatttaa ttttttgtca ggcaatacca
     1021 acattgagta tgccttaatc aacacaagta cacaaaacat tgaggcaaat aattttatca
     1081 cagatggaac agaaacagcc atcaaaatgc cttatggcat agcagtacac cctattacgc
     1141 gagatatcta tattactgat gcgaaaagtt acattattcc gggtagtgta tattgctaca
     1201 gcaaaactgg caaacgaaaa tggtacgtta ccacaggtga tattccagct catattgcat
     1261 tcttaaccaa acaaaataac taacttaact cacttaatga tgaaaagaaa tttacttgta
     1321 acgattcttt taggattgat tgtgtgtaac ttacaagcag caaattctgc ttacgtaacc
     1381 gaaattattg attatcgtcc agcacctggg caacatatca atcgttcatt tcctcctgct
     1441 gataagagtg attctcacga aaatgcggtg gcttgggcag ctactcaatt ggtaggtaaa
     1501 accaatggtt tggttggact gggagctttt ggcggatata ttattgttgg attcgaccat
     1561 tctattgtga atgtagccga agaatatgat tttaaagtat atggcaatgc ttttgctaat
     1621 ggtgcggagc caggcattgt aatggtttgt caagacttga atggcaatgg agtacccgac
     1681 aacgatgaac cttggtacga attggctggt tctgattatc aactgtcaag cactattaaa
     1741 aactatgaga ttacctacac acgacccaat cctgatttac aaaaatcgca tatccaatgg
     1801 actgataatc aaggtggaag cggtcaggta acacacatat cttttgcctc acaaagtacc
     1861 atgtacccat tgtggatagc tgacaatacg ctcacattta ggggaaccaa actagcgggc
     1921 aatgccattc agaatggttc aatgtttacc ttgcctgcct tatcgtgggg ttatgcagat
     1981 aaccaaccga ataatagcac caatgataaa actagtttta acattgactg ggcagtagat
     2041 gcatcaggca atccagttca gttgtcccat attgatttta tcaaaatcta tacagcaatg
     2101 gtacaagaag ctggttggct tggcgaaaca tcaaccgaaa tagcaggcgt ggaagatttg
     2161 caccccaatg cctcattgcc aactgtaata cattcacaag aaaataagca aatgtatatt
     2221 caaaatcctt ttaatgaaac attgattgtg aaagccaatg aaaattgtga aatgatttta
     2281 tataccatct ctggtaattt aatcggaaaa tacatgatta ctccaggcga aaatacaatt
     2341 actacatcac aacttcctac tgggaactat attgtacata ttatcaacac aaaacaaaac
     2401 catatttaca aactaataaa acattaaacc atgaaaacaa ctttactgac actgatttct
     2461 ttgcttttat tggctagcaa tacttttgcc accaatatcg tgttgaacct aagcaaccca
     2521 acaaccccag cgaacattaa ctacgacaca gaaaaaaaat actggacaga tacgtacagc
     2581 gaatcatctg actatacatg gttgcagttt caaaatttta agttagcaca cttaatcgac
     2641 caagattctt ggggcggaac atattgggac ggttttacta tctcaacaag tggtgataat
     2701 acagactata gctccactgg gtggatggga aatgaatggg gctgtatggc aggcggagga
     2761 attaaaaccg accaatcggg agctattgta actacaaatg gtgtagttga cacagaacaa
     2821 ggtataccat atcttgtagc gtactactct gcgtatgctt ctaatggcta tagtaacgaa
     2881 ataaaatttg ccgatggaca aaaatacgaa gctgtagaag tatatgttac aaaccaccct
     2941 tggccctatt acgaaaatat gaatggaggt atgtttacac gtgtcctcga ccaagaaggt
     3001 gattatgtaa aactgattgc caatggctta gacgaaaatg gaaatagcac aggttcggca
     3061 agtttctact tagctaaatt tgaaaatggc tcgttaacgc aagttaccaa ttgggaaaaa
     3121 ttcgatttat ctgcacttgg taccattgca tctctgtatt tcacaataga atcaactgac
     3181 actggcgatt ggggcatgaa tacatctaca tttttttgca tggataaatt aactgtaaaa
     3241 gaatatacta caagcatacc acaattagca accgaaaatg ctgcctatag agtcaacaat
     3301 atcctataca acataccaga aaacgcaaca ataacggtat ataacttaca aggacaactt
     3361 gtttattcca ctatggctac atcaaatact gtaagcttac ccatagttaa caatcattta
     3421 attattaaaa ttgtaaacga aaacaataca caaattatcc gataatacct tactgtgtct
     3481 aaaacctgtt acgtttgatt ctttctgcca taattgcatc aaattacgta acaggttttt
     3541 cttatttttt aaaaattatt ttcttatgga aatatttttt atatcaaatt tattttctac
     3601 ttttgtgtta agtaaaaaaa agaatcactc agcacaattt acaatgaata tatatacatt
     3661 aaaaaccatc cttattgcca cactactagc agcaatagga ttttcaggtt ttgcacaagc
     3721 agaacaagga ggtgcattac gaggtagggt ttacaatcca gacaacaacc aaccggttga
     3781 ttttgcgtat gtcattatcg atggcactac cattggtact attacggact tagacggtaa
     3841 ttttgctatc tttggtttaa aacctggtta tgtacgggta gcagttaatt cgcttggatt
     3901 cgaccctgtg gtaacgaaag aaattctaat taccaacgct aacacgccgt atatcgaaat
     3961 tgagatgaaa ccatcaaaca tacagttaga tgatgtggtg gtagaagcat ctcgttttag
     4021 gcaacgcgaa gaaagtccac tcagtttacg ctccatcggc atcaaagaaa tagaaaaaag
     4081 cccaggcggt aatcgcgata tttctaaagt aattcaatct cttccaggag taggtgctac
     4141 cccagccaac cgcaacgata ttattgtgcg tggtggtggt gccaacgaaa accgtttttt
     4201 tctcgatggc gtagaaatac ccaacatcaa ccactttgct acacaaggtt caagtggcgg
     4261 accggcaggg attatcaacg tagattttgt acgcgagctc gacttttttg cgggagcatt
     4321 tcctgccaac aggggcaatg cattgagttc tgtactcgat tttcggttga tagatggcaa
     4381 taaagaccgt ttaaaaacac gtgccacagt aggtgctagc gatttggcac ttacgcttga
     4441 tggtcccata ggcgataaaa gttcgttcgt attttcggta agacgctcgt atttagaatt
     4501 tcttttcgac ctgattggac tgcccttttt gcctacttac aacgatatac aatttaagca
     4561 taaaattcgt tttaaccaaa acaatgaact aagctttatt ggcttaggtg cttttgatgt
     4621 aaacagatta aacctaagtg ccaacgaaac agaaagccaa cgttttatac ttaaatacct
     4681 gcctaccaac gaccaatgga catataccgt aggggctgtg taccgcaatt tctttaaaaa
     4741 cggtaaccaa accttggttt tgagtcgcaa ccacctcaac aatgtatcgc aaaaatacca
     4801 agacaacatc gaagcaccta ctaacctcaa ttttgactat gcctcgaacg agatagaaac
     4861 aaaaattagg tacgaatatg taacacgtca aggtgcgtgg aaatttttgt atggagctgg
     4921 tgccgaatac aatcaatata ccaattatac ctacaacaaa ttatttttca atgcacaagt
     4981 agtagagttt gattacgaaa ctgagctcga cttctattcc tattcggcat ttacacaaat
     5041 cagccgcaat ttactcgctg agcgattact cgtatcagca ggagcccgca tagatggcaa
     5101 cacctacagc gaacaaatgt caaacccatt ggaacaattc tctccacgta tttcggctag
     5161 ttatgcatta acaaataaat ggtcgttaaa tgccaataca ggacgctact accaacgtcc
     5221 agcttatacc accatgggat acagagatat agcgggtaat ctaattaata aagataaccg
     5281 tcttcgctac aaatataccg accattttgt agtgggagta gaaaataaac caaacccaaa
     5341 tagccggttt acagtagaag gtttttacaa actttacaat gattatcctt tctccatcag
     5401 cgattcagta gctatctcat ccaaaagtgc cgattttgat gtatttggag cagaagcggt
     5461 tttgtctatt ggcgaaggtc gcacctatgg ggtagaaata ctgtatcgca atacggattt
     5521 atggggaagc aatattacca ccagttttac cttattccgt agcgaattta aagactctaa
     5581 agaagtgtat atccccacag catgggacaa tcgtatattg ttcaatctta ccgccaccag
     5641 accattgccc aaaaactggg atatagggtt taaatggcgt tatgtgggag gtgctcctta
     5701 tactcctgta gacttagaac gctctagttt gcgacaagca tacgatgtaa caggcagaac
     5761 gtatttggat tacgatagat ttaattccga acgattagct ccttcgcatc aattggatgt
     5821 acgtgttgat aaagcgttct ttttcaacaa atggtcgctg atgctttatt tggatataca
     5881 aaatatctat aatttcaaaa gccaacaacc cgaccaatac atcaacgaag acaaagatgg
     5941 aaatgtggta atcgaaaacc caacggcacc gctcaacgag caacggtatg tattacgcaa
     6001 actcgaaaat acaggcagtg gcacagtatt gccttccatt ggaattatga tagaatttta
     6061 attatataac aagactaaat agctcactaa aaacgaactc gtatgactac aaaaaaatat
     6121 atactactta ttgctagcct actgctaagc acagcttatg tggcagcaaa caagcaaacg
     6181 ctatcaacca ttgaactgag tgtggaaaaa ggcaaaagtt acaactaccc tacttttgcg
     6241 ttgtggctcg aaagtgctgg aggcaaattt gtgcaagaat tgtacgttac acaatcctta
     6301 gctacaggca tctacaacaa tgctgacgct ggcgataaac gctggaaaag agaaaaagga
     6361 gaagctcgac gcccgtcaac actgccttat tttttgcata aacgcgacat acaagcacct
     6421 gacggcacgt acttaccaac acccgaacaa cccatacccg atgcttatac aggagctact
     6481 cctcgccaat ctgatgtaat aaccatcgtg ttagatagac aattatacgg aaaatacaga
     6541 attctatttg agattaacca accttgggat tttaaccgtg tgtggtacaa caaccgctac
     6601 ccaaacaata ccgactacct gctttcgggg caaccatcgt tggtatatat ggtagaaata
     6661 gatacgaaca atttgcaaga ggaatacact ctcaacccga ttggtcatgg acatccgtat
     6721 ggtaaaacag gtaccttatt tacagatatt tcgggcatta ccactgccaa aaacatattt
     6781 aaatcgatta aagtaaaggt atattaagca cacacaaaac ccaatagatg caatgagagg
     6841 aagagcgatt tttctctcat tgttgtatca ataccgattg agcaaggctt tgggcgaacc
     6901 tatccacgag caacccaaac aatggagttc gtcagtaaaa tacccctcga gcgatacacc
     6961 ctcgtgaatg ggtataacct catagtgttc gtttacagtt accaccaccg actcgcgggc
     7021 tgcattaaaa acacgaaacc gatggatacg acattgtggg caaaggagtt ttttcatact
     7081 agtaaaaaca ttaatttggt gcaaagatac tttttctttg tatatacacg catatcaaat
     7141 gcttactttg cattattttg cgtacttttg atagcaaata atacacccat ttatgaaaac
     7201 agcacttatc attggtagca caggattggt tggttcggct ttgctcaacg aactattggc
     7261 acatccagcc tacagcaagg ttatttcatt tacccgcaaa cccagtggca acatacaccc
     7321 aaaactacac gaacattgca tcgactttaa taacattgcc tcgtacaaag acttgataca
     7381 aggcgatgac ttgtactgtg ccatgggtac caccatcaaa aaagcaggta gccaagccgc
     7441 atttactttg gttgattata cctaccctta ccaatttgcc accatcgcca aaagcaatgg
     7501 ggtgaaacaa tttttattgg tatcctccat aggagccaat gcccaatcca aaaacttcta
     7561 tttgcaaacc aaaggaacga ttgaaaacgc cattagccta ctgggatttg aaacggtggt
     7621 cattttccga ccttccttgc tactaggcaa acgcacagaa tttcggtttg gagaaaaaat
     7681 agccatgcac ataagccaat tgttctcgtt tctgtttgtt ggcaaactac gcccctacgc
     7741 ccccatccat agccacaccg tagccaaagc tatggtaagc gtagcacagc aagcaaacac
     7801 aggcgtaaac atatatgctt ctgatgctat aaaagaggta taaacaaatt tttattggtt
     7861 gggtatattg ttttctattt acttgtagga tgtttttttt ttgtattttt aaaaaattgt
     7921 atatctttgt taaatattat atactattaa ttacaatcta ataaaacaaa agattatgac
     7981 gtaacttact ttgaattact aatatagaag cgttagcttt tattcttgca gttgaaaatc
     8041 gccagtttaa tacgcataca
//
LOCUS       DAOSRP010000092         8041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8465_length_8041_cov_4.056975, whole genome shotgun sequence.
ACCESSION   DAOSRP010000092 DAOSRP010000000
VERSION     DAOSRP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8465_length_8041_cov_4.056975"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLJ82_06530"
     CDS             complement(<1..453)
                     /locus_tag="PLJ82_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41196.1"
                     /translation="MRNTLLIVAFAIVALIFSIWGYNALRANSVSGKIDVYTAVPMQP
                     IAMLHINNIAELQSSLLYNNTYWLDVSKLEAIAPMHAIFMKTDSLKDVSSDIKEFLSR
                     RETLISIFADSLSTHSLVSMSISKNDWKMMKKGFLKNLFPHYKSEEITI"
     gene            complement(478..1701)
                     /locus_tag="PLJ82_06535"
     CDS             complement(478..1701)
                     /locus_tag="PLJ82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOR41197.1"
                     /translation="MIKKAPCILLLVYTFCSCIMAENFTPYKIKLQGIGGYIHPHDEN
                     VEVLVHGPALGGEIALELGTDGSESWHIHHNFPDIGIAAQYLTLSNPEILGEMISLYP
                     YINFPMVVTDWFSFYLKLGGGVALATKPTDIEGAIEANRPIRGANGLNKDFNFVIGST
                     ANVILAGGATMEVRLSRNLRFTSDLIFNHYSNGNTVQPNMGLNLANVYVGLKYIPIYD
                     GYVARGLARVPSTHDRFSGEVIFSGGSRQRYYKDTKSFPIASLNLGGYYHTCHQHRIG
                     IGLDGFYNGIFTNDPALSNSFRRTNITTDELKNKLRLGINLANDVLVGRFTAGLQFGV
                     YLYDPIKDLEPYNPASRKDKGLIYAYDIEKEDGWLYTRLSAKYRIIDHIIANISIKTH
                     LHKAEFIEFGLGYAF"
     gene            1806..2534
                     /gene="gldF"
                     /locus_tag="PLJ82_06540"
     CDS             1806..2534
                     /gene="gldF"
                     /locus_tag="PLJ82_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOR41198.1"
                     /translation="MYVLFIKEIRSFFSSAIGFIVIGIFLLLMGTFLWLLPGENNVLE
                     SGYANVDGLFLLAPWLYLFLIPALTMRFFAEEKKSGTIELLLTRPLSTLQIVLAKYFA
                     GVVLVVLSLLPTLVYVYSVYQLGLPQGNIDMGGFLGSFIGLIFLGAGYTAIGLWASSV
                     SDNQIVSFLVAALLSFLLYYGFELLSLFFTSAYVQDMVSQMGMSYHYDSMSRGVIDSR
                     DVLYFVTISVVFLFATTWFVTKRK"
     gene            2598..4322
                     /gene="gldG"
                     /locus_tag="PLJ82_06545"
     CDS             2598..4322
                     /gene="gldG"
                     /locus_tag="PLJ82_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOR41199.1"
                     /translation="MKRPILITLVVLVLLHVVSAFYFFRIDLTAEQRYTLSPNTKKLL
                     RTLDNTMDVTIYLSGDLNMGFLRLQKATTEMLQEFSRYSEQPIAIQMVNPSKASNEKA
                     RFLQYNALEQQGMVPTIVNEKDAEGKFIQKVVFPWAEMVYQGDTVRVNLLKNIPGKIG
                     DENLHISIESLEYELTDAIRRLANKHTAERVAFIEGHGELSEMQVFDITAALAQYYHV
                     DRGQIGADVRILDEYKVVIVAGATERYTESEKFILDQYIMNGGRVLWLLDGVRTSQDA
                     LTTSATTIGIVNDVNLDDQLFTYGIRIAPILLADKQCAQVRVNVATMGQTPQFELAPW
                     YYFPLLQTSPYHVITKNLSPVRAEFASAVQFVGENNTVTKELLLASSTATGIQTAPME
                     LSLSMINNPPSDEFFQYSFVPVAAIMSGSFESVFTNRMVPQDVIVPQVYKIRTKSNKT
                     KMVVVADADIIRNEVQVSANDTVALPLGYDRHVNKQYGNRDFILNTVNYLADDEGWMN
                     LRAREFTLRLLNKQAIESQRVFWQIINVVLPLVLLIVCILLYQRSRKMRYGNTRKSSS
                     IKAFFTKK"
     gene            4397..5524
                     /gene="dnaN"
                     /locus_tag="PLJ82_06550"
     CDS             4397..5524
                     /gene="dnaN"
                     /locus_tag="PLJ82_06550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOR41200.1"
                     /translation="MKFAVSSSHLYERLSSVSRVINTKNTLPILNYFLFSIQGKELSI
                     TASDMETTLITSMELEEVEGEGKVAILAKNLIDILREFADQPLTITIDTASFSVVITS
                     AQGKFSFIGQSGEEYPKMHELDADNTVSFVAPASVLLDGISSTVFATADDELRPVMNG
                     ICFDVTTEELTLVGTDAHKLARFKTTAIKGEKDAMFVLPKKPASLLKSILVKETAPVN
                     VSFDTKNSIFTLSNYKMVCRQIDARYPNYNAVIPKSGPTKIIVDRLALLSGVKRVSVC
                     TNQASSLIRFAISDNQIYLSGQDIDFSISGEEYIPCQFQGEAMEIGFKASFIIELLSN
                     INASDVEFTLIDRTRPGIFTPAEQDDNKNILMLLMPMMLND"
     gene            5544..6746
                     /locus_tag="PLJ82_06555"
     CDS             5544..6746
                     /locus_tag="PLJ82_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOR41201.1"
                     /translation="MSKVVIIGAGGVGTVVAHKVAQNSKVFTEVLLASRTKSKCDAIA
                     ADVQSRYGVTMQTAQVDADNVPELVALLRSFQPKLVINVALPYQDLTIMDACLEVGVN
                     YLDTANYEPKDEAHFEYSWQWAYQDKFKKAGLTAILGCGFDPGVTSVFTAYAAKHHFD
                     EMHYLDIVDCNAGDHGKAFATNFNPEINIREVSQRGKYWENGKWVETEPHEIHKPLNY
                     PEIGPKESYVIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMARIDEVE
                     YNGQKIVPIQFLKAVLPNPGDLGENYTGWTSIGCRIKGIKNGEEKTYYIYNNCSHEEA
                     YKETGTQGVSYTTGVPATVGAMMFLTGEWGGAGVFNVEEFNPDPFLHELAQQGLPLHE
                     LHNIDLEL"
     gene            7029..>8041
                     /locus_tag="PLJ82_06560"
     CDS             7029..>8041
                     /locus_tag="PLJ82_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOR41202.1"
                     /translation="MKTSIHLTLILLLFFGSFHLTAGTYSGGNGTVNDPYQIATYADL
                     LELSATSADWATGKYFIQTAHIDASATATLNGGKGFVPIGNLDYKFQGSYDGQHYTIS
                     NLYINRSDEDRIAMFGYLWGAGISVQNLGLTNVSITGKNSVAALAGYNRDGIINNCYS
                     SGSITGYSSIGGLVATNYMATITNSYSTCSVSGTGTMGGCVGVNNQSVISRCYATGSV
                     TGLSVLGGFVGSNKASDTKAATISHSYSTGNVVRTAAPNVKIGGFCGTNELKGAIQNC
                     YSTGSVNFGTAQGFLGENITNATFSGNFYDSQTSKQTTGTGATARTTAQMKTESTFTN
                     WDFT"
BASE COUNT         2468 a         1376 c         1652 g         2545 t
ORIGIN      
        1 tatggttatt tcttctgatt tatagtgagg aaaaagattt ttaaggaatc cttttttcat
       61 cattttccaa tcatttttag aaatactcat acttactaaa gagtgcgtag ataagctatc
      121 agcaaaaata ctaatcagtg tttcgcgacg acttaaaaac tcttttatat ccgaagaaac
      181 atcttttaat gagtctgttt tcataaaaat agcatgcata ggagctatgg cttccaattt
      241 acttacatca agccagtaag tattgttgta gagcaacgag ctttgtaatt cggctatatt
      301 attaatgtgc aacatagcaa ttggttgcat aggaacagcg gtatacacgt caatttttcc
      361 tgatacagaa ttggctctca aggcattgta tccccaaatt gagaaaatta gagcaactat
      421 ggcaaatgca actattaata aggtatttct catgtgtgtt tattttcttt atgtcaatta
      481 aaaagcatac cctagcccaa actcaataaa ttcagccttg tgtaagtgcg ttttgatact
      541 tatgttcgca ataatatggt cgataatgcg gtacttagca ctaaggcgtg tgtatagcca
      601 accgtcttct ttttctatgt cataggcata aatcaatcct ttatctttgc ggctggcagg
      661 gttatatggt tctaaatctt taatagggtc gtataggtac accccaaatt gtagcccagc
      721 agtaaaacga cctactagta catcattggc aaggttaatt cccaatcgga gtttattttt
      781 taattcgtct gtagttatgt tagtgcgtct aaatgaatta gacaaagcag gatcgttggt
      841 aaatatgcca ttataaaaac catctaaccc tatacctata cggtgttggt ggcaggtgtg
      901 gtagtaacca cctaaattga gagaggcgat aggaaaactt ttggtgtctt tgtaatagcg
      961 ttgacggcta cctcccgaaa aaataacttc gcccgaaaat ctgtcgtgcg tactaggtac
     1021 acgagctaat ccgcgtgcca cataaccatc atagatagga atatatttta atcctacata
     1081 tacattggca agatttaagc ccatattcgg ttgcacggta tttccgttag agtaatgatt
     1141 gaagataagg tctgaggtaa aacgtaggtt gcgtgataaa cgcacttcca tagtggctcc
     1201 acctgctaaa attacattgg cagtgctacc aattacaaaa ttaaagtctt tatttaagcc
     1261 atttgctcca cgaataggac gattagcttc aatggcacct tctatatctg tgggtttggt
     1321 agctagggct acaccgcccc ctaatttgag gtaaaacgaa aaccaatcgg taactaccat
     1381 tgggaagttg atatacggat ataggcttat catttcgcct aagatttctg gattactcag
     1441 cgtaaggtat tgagctgcaa tgccaatatc aggaaaattg tggtgaatat gccaactttc
     1501 ggagccatca gtgcctagtt cgagggcaat ttcgccacct aaagctggtc catgcacaag
     1561 tacctccaca ttttcatcgt gtggatgaat gtagccacct ataccttgta atttaatttt
     1621 atacggagta aaattttccg ccattataca actacagaat gtatatacta gtaggagtat
     1681 acatggtgct ttttttatca ttgttcgtgt tttgagttcc catacaaaat gcgtatcttt
     1741 gtgcttgttc gcaataagcg aaattcgatt acgcaaattt acatatttta tactaaaaac
     1801 aagctatgta cgtattgttt attaaagaaa tccgttcatt tttttcatcc gctatcggct
     1861 ttattgtgat aggtattttt ttattgctta tgggcacatt cctatggtta ctacctggcg
     1921 aaaacaatgt gcttgaaagt ggttatgcca atgttgatgg attgtttttg ttggctccat
     1981 ggttgtattt atttttgata ccagcactta caatgcgttt ttttgcagaa gaaaaaaaat
     2041 cgggtactat cgaattgtta cttacacgac cactcagtac tttgcagatt gtattagcca
     2101 aatattttgc aggcgtagta ttggttgtgt tgtctttgct acctaccttg gtatatgtgt
     2161 atagtgtgta tcagttgggc ttgccacaag gcaatatcga tatgggtgga tttttgggtt
     2221 cttttattgg acttattttt ttaggagctg gttatacggc tattgggtta tgggcttcgt
     2281 ctgttagtga taaccagatt gtatcttttc ttgtggcagc tttgttgtca tttttattgt
     2341 attatggttt tgaattacta tctctatttt ttacttcggc atatgtgcaa gatatggtat
     2401 cgcaaatggg catgagctac cattacgatt caatgagtcg tggtgtaatt gatagtagag
     2461 atgtgttgta ttttgttacc atcagtgttg tttttctgtt tgctactact tggtttgtga
     2521 ctaaaagaaa atagcaaggg agtgaaaaag gaatgcaaaa aacgaaattt accttagaga
     2581 aagcataaaa aatgtatatg aaaagaccta ttctcataac gcttgttgta ttagtgttgc
     2641 tacatgtagt atctgcattt tatttttttc gcattgactt aacagcagag caacgctata
     2701 ccttgtcgcc aaatactaaa aaattactac gtaccctaga taatacaatg gatgtaacca
     2761 tttatttatc aggcgatttg aatatgggtt ttttgcgatt gcaaaaagcg actactgaaa
     2821 tgttacaaga gttttctcgt tattccgagc aacccatagc catacagatg gttaatccat
     2881 ccaaagcgag caacgaaaag gctcgttttt tgcaatacaa tgcgttggag caacaaggta
     2941 tggtgcctac tattgtaaac gaaaaagatg ctgaggggaa atttatacaa aaagtagttt
     3001 ttccatgggc agaaatggtg tatcaaggag atactgtacg tgttaatttg cttaaaaaca
     3061 tacctggtaa aataggtgat gagaatttgc atatttcgat tgaaagtctt gaatatgagt
     3121 tgacagatgc tattcgtagg ttggcaaata aacatactgc cgaacgtgtg gcgtttatcg
     3181 agggacatgg cgaattgtcc gaaatgcagg tgtttgacat cacagccgct ttggcacagt
     3241 attaccatgt agatagaggg caaattggtg ctgatgtgcg aattttagat gaatataagg
     3301 tggtaattgt agctggagca acagaacgtt acaccgaatc agagaaattt attctcgacc
     3361 aatatattat gaatggcgga cgcgtacttt ggttgctcga tggtgtgcga acatcgcaag
     3421 atgctcttac tacatccgct acaacgatag ggatcgtcaa tgatgtgaat ttggacgatc
     3481 agctttttac ctatggtatc cgtattgctc ctattttact agctgacaaa caatgtgctc
     3541 aagtgcgtgt taatgttgcc actatggggc aaacgcctca gtttgaactg gctccgtggt
     3601 attatttccc cttgttacaa acatccccat atcatgttat taccaaaaac ctctcgcctg
     3661 tgcgtgccga gtttgcttct gccgtacaat ttgtaggcga aaataacaca gtaacaaaag
     3721 aattattgct agcaagctct actgctacgg gtatccaaac ggctcccatg gagttaagct
     3781 tgtcgatgat taataatccg ccttctgacg agttttttca gtatagtttt gtgcctgtag
     3841 cggctattat gtcgggtagt tttgagtcgg tatttaccaa ccgcatggtg ccccaagatg
     3901 tcatagtgcc ccaagtatat aaaattagaa ccaagagcaa taaaacaaaa atggtagtag
     3961 tggctgatgc tgatattatt cgtaacgaag tgcaagtgtc tgctaacgat acagttgcat
     4021 tgcctttagg ctacgatagg cacgtaaata agcaatacgg aaatcgcgac tttattttaa
     4081 ataccgtgaa ttatttagct gacgacgaag gatggatgaa tttgcgtgca agggaattta
     4141 cattacgttt gttaaacaaa caagccattg aatcacaacg tgttttttgg cagattatca
     4201 atgtagtgtt gccattagtt ttgctcattg tttgtatctt gttgtatcaa agaagtagaa
     4261 aaatgcgtta tggaaatacg cgaaaatctt catcgataaa agcttttttt actaaaaaat
     4321 aatgtatatt tgtcaactga ttttagacaa tatagataaa taagtatcga taaactaaat
     4381 ttaaaactac atacaaatga aatttgcagt atcatctagc cacctttacg aaagactaag
     4441 ttcagttagt cgcgttatta acactaaaaa taccttgcca attctaaatt attttttgtt
     4501 ttctatacaa ggcaaagagc ttagtatcac cgcttcggat atggaaacaa cccttattac
     4561 atcaatggag ttagaagagg tagagggaga agggaaagta gccatcttgg caaaaaactt
     4621 aatagatatt ttacgcgaat ttgccgatca accacttact attaccattg atacagctag
     4681 tttttcggta gtgattacaa gtgcacaagg taaatttagt tttattggac aatcgggcga
     4741 agaatatcct aagatgcacg aactggatgc agataatacc gtttcttttg ttgctccagc
     4801 tagcgttttg ttagatggta tatcgagtac agtgtttgct acggcagatg acgaattgcg
     4861 tcctgtaatg aatggtattt gttttgatgt aaccactgag gaacttacct tggtaggtac
     4921 ggatgctcat aagttggcac gttttaaaac aactgccatc aaaggcgaaa aagatgctat
     4981 gtttgtattg cccaaaaaac cagcctcatt gctcaaaagt attttagtaa aagaaacagc
     5041 accagtaaac gtatcgtttg atacaaaaaa ttcaattttt accttatcaa attacaaaat
     5101 ggtgtgtcgc caaatagatg ctcgttaccc gaattacaat gctgttatac ccaaaagcgg
     5161 acctaccaag attattgttg atagattggc gttgctttct ggtgtaaaaa gagtatctgt
     5221 atgtactaat caagccagta gtttaatccg ttttgctatc tctgataatc aaatttattt
     5281 atcgggtcaa gatattgatt tctctatttc aggagaagaa tatatccctt gtcaattcca
     5341 aggagaagct atggagattg ggttcaaagc tagttttatc atcgaattat tgagcaatat
     5401 caatgcttcg gatgttgaat ttacgcttat tgaccgtaca cgtcctggta tttttacacc
     5461 agccgaacaa gatgataata aaaatatatt gatgttgttg atgccaatga tgctaaacga
     5521 ttaaacaata acagaaaagt aatatgtcaa aagtagtaat tataggagca ggcggtgtag
     5581 gtacggtagt agcacacaaa gtagctcaaa atagcaaagt gtttacagag gtattgttag
     5641 ctagccgcac caaatcaaaa tgtgatgcca ttgctgccga tgtgcaatcg cgttatggtg
     5701 taactatgca aactgcacaa gtagatgccg ataatgtgcc cgaattagtc gctttgttgc
     5761 gttcttttca gccaaaatta gtcattaatg ttgctcttcc ataccaagat ttaactatca
     5821 tggatgcgtg tttggaagta ggtgtaaatt acctagatac agccaactac gaacccaaag
     5881 acgaggctca ttttgagtac tcgtggcaat gggcttacca agataaattt aaaaaagcgg
     5941 gtttaactgc cattttaggt tgtggtttcg accctggtgt aacaagcgta tttactgcct
     6001 atgcagccaa acatcatttc gatgagatgc attatttaga tattgtcgat tgcaatgctg
     6061 gcgaccatgg caaagcattt gctaccaatt ttaaccccga aatcaatatc cgtgaagtga
     6121 gccaacgagg caaatattgg gaaaatggca aatgggtaga aacagaacca cacgaaattc
     6181 ataaaccact gaattatcca gaaattggtc caaaagaatc ttacgttatc taccacgaag
     6241 agttggagtc gctagtaaag aattttccga ccctgaaaag agcccgtttt tggatgacat
     6301 ttggacaaga atatttaact cacttacgtg tgattcagaa tattggtatg gctcgcattg
     6361 atgaggtgga atacaatgga cagaaaattg tgccaattca atttttaaaa gctgtattgc
     6421 ctaatccagg cgatttgggc gaaaattata caggatggac atctatcggt tgtcgcatta
     6481 agggtattaa aaatggagaa gaaaaaacgt attacatcta taataattgt agccacgaag
     6541 aggcatacaa agaaacaggc acacaagggg taagttatac cacaggtgta ccagccacag
     6601 taggtgctat gatgttttta actggcgaat ggggtggagc aggtgtattt aatgtagaag
     6661 agtttaatcc agatccattt ttgcacgaat tagcacagca aggcttgcct ttgcacgaat
     6721 tgcataacat tgatttagaa ttataacgcc aataagggtc tacattttat taaacagcac
     6781 gataaattac tatgtgctgt tttttttttg ctctggagtg tttgttattc gtgttggttg
     6841 caatatagtt aatattcgat aaaaatttgg gtaatctgtt gtaaatataa ataattagat
     6901 agatttttgt tataaatttt ataaaaccag tatgtggtag gtcgtctact taatggtata
     6961 tatgacgtat gtaatttcat aaaagccata agaccactta cattatgaac aagacaataa
     7021 aaaactatat gaaaacatca atacacctta cgttgattct acttcttttt ttcggaagtt
     7081 ttcatcttac tgccggaact tattctggag gcaatggcac agtaaatgac ccctaccaaa
     7141 ttgctacata tgccgattta cttgagctat ctgccacctc tgccgattgg gcaacaggca
     7201 aatattttat acaaactgca catattgatg ccagtgctac tgctacattg aatgggggta
     7261 agggttttgt ccctattgga aatttagact ataaatttca ggggagctac gatggtcaac
     7321 actataccat atctaatttg tatatcaacc gaagtgatga agatagaatt gccatgtttg
     7381 gttatctatg gggagcgggt attagcgttc aaaatctggg tctaaccaat gtttctataa
     7441 cagggaaaaa ctccgtggca gctttagcgg gatataatcg tgatggcatt ataaacaact
     7501 gctattcttc aggtagtata actggttatt cttctatagg aggattagtg gcaacaaact
     7561 acatggctac tataacaaat agttattcta catgcagtgt cagtggcacg ggtacaatgg
     7621 gtggatgtgt aggtgtaaat aatcaaagtg ttatttcacg ttgttatgca acaggtagtg
     7681 ttactggatt aagtgtgctt ggcggatttg ttggtagtaa caaagcaagt gataccaagg
     7741 cggcaaccat tagtcattcg tatagtacag gcaatgtggt tcgaactgct gcacccaatg
     7801 tgaaaattgg tggattttgt ggtactaacg aactgaaagg agctattcag aattgctatt
     7861 ctactggctc ggtaaatttt ggcacagctc aaggcttttt gggtgagaat ataaccaatg
     7921 caactttttc gggtaatttt tacgattcac aaacaagcaa acaaactacg ggtactggtg
     7981 ctacagcacg cactacggct caaatgaaaa ctgaatctac ttttactaat tgggatttta
     8041 c
//
LOCUS       DAOSRP010000093         7976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8541_length_7976_cov_5.539705, whole genome shotgun sequence.
ACCESSION   DAOSRP010000093 DAOSRP010000000
VERSION     DAOSRP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_7976_cov_5.539705"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..128
                     /locus_tag="PLJ82_06565"
     tRNA            56..128
                     /locus_tag="PLJ82_06565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Thr,seq:ggt)
     gene            192..1379
                     /gene="tuf"
                     /locus_tag="PLJ82_06570"
     CDS             192..1379
                     /gene="tuf"
                     /locus_tag="PLJ82_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR41203.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITTVLAKKGLSEL
                     RSFDSIDNAPEEKERGITINTSHVEYQTANRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRIVVFMNKCDSVDDEEMLELVEMEMRELLSF
                     YDFDGDNTPIIRGSALGALNGVAQWEDKVMELMDAVDSWIELPPRAIDKPFLMPVEDV
                     FSITGRGTVATGRIETGVIKVGEEIQIIGLGAGAKKSVVTGVEMFRKLLDQGEAGDNV
                     GLLLRGVDKEEIKRGMVITHPGKVTPHTSFKASVYIMKKEEGGRHTPFHNKYRPQFFI
                     RTLDVTGEITLPDGVEMVMPGDNLEITVNLIYPVACSVGLRFAIREGGRTVGSGQITE
                     IIA"
     gene            1426..1498
                     /locus_tag="PLJ82_06575"
     tRNA            1426..1498
                     /locus_tag="PLJ82_06575"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1459..1461,aa:Trp,seq:cca)
     gene            1724..2272
                     /gene="nusG"
                     /locus_tag="PLJ82_06580"
     CDS             1724..2272
                     /gene="nusG"
                     /locus_tag="PLJ82_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR41204.1"
                     /translation="MSEMDKKWYVLRAISGKENKVREYIESEMKNTTLSQYVFQVLIP
                     TEKVYQIRNGKKTIKERSYLPGYVLVEATLTGEVVHQLKNLPNVIGFLGDKAGDPIPL
                     RQSEVNRILGTVDLLQQSEEEINIPYYVGENVKVISGPFNDFSGVIEEVNNEKKKLKV
                     MVLIFGRKTPLELSFMQVEKEQ"
     gene            2328..2771
                     /gene="rplK"
                     /locus_tag="PLJ82_06585"
     CDS             2328..2771
                     /gene="rplK"
                     /locus_tag="PLJ82_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR41205.1"
                     /translation="MAKEVAGLIKLQVKGGAANPSPPIGPALGAKGINIMEFCKQFNA
                     RTQDKAGKILPVVITYYSDKSFDFIIKTPPVAIQLLEVSKLKGGSAEPNRKKLAEITW
                     DQVKAIATDKMEDLNCFTIDSAMRMVAGTARSMGITIKGEFPSSN"
     gene            2789..3481
                     /gene="rplA"
                     /locus_tag="PLJ82_06590"
     CDS             2789..3481
                     /gene="rplA"
                     /locus_tag="PLJ82_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR41206.1"
                     /translation="MSKLTKNQKLALEKIEVGKNYSLKEAASLVKDVTNTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPHGTGKTVRVLVLCTPDAEAEAKEAGAEYVGLDEYIE
                     KIKGGWTDIDVIITMPSIMGKIGALGRVLGPRGLMPNPKSGTVTNEVGKAVKEIKQGK
                     IDFKVDKFGIVHTSVGKVSFTADKLVDNAKEFISTLNKLKPTAAKGVYIKSIYLSSTM
                     SPGIKIDPKSVE"
     gene            3501..4025
                     /gene="rplJ"
                     /locus_tag="PLJ82_06595"
     CDS             3501..4025
                     /gene="rplJ"
                     /locus_tag="PLJ82_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR41207.1"
                     /translation="MKKEDKSQIIAQIKSTIEAYNCFYVTDIADLNSEKTSNLRKLCF
                     NQNIKLLVVKNTLFKKALEDMGGDFSPLFGTLKGSTSIMFSNNSNSPAKLIKEFKKDK
                     KNNGKPVLKAAYVQECFYVGEDQLESLITLKSKEQLLGEIVGLLQSPAKNVISALKSG
                     GNTLHGVLETLSNK"
     gene            4073..4453
                     /gene="rplL"
                     /locus_tag="PLJ82_06600"
     CDS             4073..4453
                     /gene="rplL"
                     /locus_tag="PLJ82_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOR41208.1"
                     /translation="MADLKVFAEQLVNLTVKEVNELAQILKDEYGIEPAAAAVAVAGP
                     AVAAEAAPAEEEKSSFDVILKSAGANKLAIVKLVKELTGLGLKEAKDIVDAAPSAVKE
                     GVGKDEAEGLKKQLTEAGAEVELK"
     gene            4562..>7976
                     /gene="rpoB"
                     /locus_tag="PLJ82_06605"
     CDS             4562..>7976
                     /gene="rpoB"
                     /locus_tag="PLJ82_06605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR41209.1"
                     /translation="MSYPMSNQRINFSSIKKSLDYPDFLEVQLKSFKDFFQLDTPPEK
                     RKNDGLYRVFAENFPITDTRNNFNLEFLDYYIDPPRYSIQECMERGLTYSVPLKAKLK
                     LYCTDPDHEDFDTVIQDVYLGPIPYMTEKGTFVINGAERVVVSQLHRSPGVFFGQSLH
                     TNGTKLYSARIIPFKGSWIEFTTDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFNLAEEIKATKTNLKKAVGRKLAARVLKTWIEDFVDEDTGEVVSIERNEVIIDRETI
                     LDNDHVNEILEANVQTVLLHREDLSSSDYSIIYNTLQKDPSNSNKEAILYIYRQLRNA
                     EPPDDASAREVIYNLFFSEKRYDLGDVGRFRINKKLDLDISPEIKVLTKEDIIAIIRY
                     LIELINSKADVDDIDHLSNRRVRTVGEQLCNQFSVGLARMARTIRERMNVRDNEVFTP
                     IDLINAKAISSVINSFFGTNALSQFMDQTNPLAEMTHKRRMSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIETPYRKVDDKKVNLNKED
                     IIYLSAEEEENIIIAQGNAPLDDNGNFTRTRVKARQDSDFPIVAPEEVQLMDVSPTQI
                     ASIAASLIPFLEHDDANRALMGSNMMRQAVPLLRCEAPIVGTGLEGQIIKDSRTQITA
                     ERDGVIEYVDATTIKVRYNYSEEELFVQFESSVKEYTLPKFQKTNQSTTIDLRPIVHK
                     GEKVVAGQILTEGYSTQQGELAIGKNLKVAFMPWKGYNFEDAIVINERIVREDVFTSI
                     HVDEYSLEVRETKRGLEELTADIPNVSEDATKNLDENGIVRIGALIEPGDILIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKAHPSLRGVVIEKRLFSKAQKAKKSKSAD
                     KAIMDKIEAEFEEKAHALKLKLVEKLLVLTNGKTSQGVKDFLGAEVISKGSKFTQKQL
                     LDVDYSTVQISKWTSDSAKNEQIKTLIMNYLKKYKEYDAQLKREKYNITIGDELPAGI
                     IQLAKVYIAKKRKVRVGDKMAGRHGNKGIVSRIVRQEDMPFLEDGSPVDIVLNPLGVP
                     SRMNLGQIFETVLGWAGKELNVKFSTPIFDGATLEDLDEWTTKANIPRYG"
BASE COUNT         2838 a         1245 c         1575 g         2318 t
ORIGIN      
        1 tagccttcca agctgagggt cgcgggtttg agtcccgttt tccgctcttt tttttgccga
       61 tgtagctcag ctggcagagt gcgtccttgg taaggacgag gtcacgggtt caagtcccgt
      121 cattggctct gacaaatgta aaaaatgttt aaaaactaat ataatctcta actaacaaaa
      181 aaattaacgt tatggctaaa gaaaaatttg acagatcgaa accacacgtt aacgtaggaa
      241 caattggcca cgttgaccat ggtaaaacta ctttaactgc tgctattaca acagtattag
      301 caaaaaaagg tctttcagaa ttgcgttcat tcgattctat cgacaatgct cctgaagaaa
      361 aagaaagagg tattacaatc aatacttctc acgtagaata ccaaacagca aatcgccact
      421 atgcacacgt tgactgtcca ggtcacgctg actacgttaa aaacatggta actggtgctg
      481 ctcaaatgga cggtgctatc atcgtagtag ctgcaaccga tggtcctatg cctcaaactc
      541 gcgagcacat ccttttggct cgtcaggtaa acgttcctcg tatcgtagta tttatgaata
      601 aatgtgatag tgttgacgac gaagaaatgt tggaacttgt tgaaatggaa atgcgtgaat
      661 tattatcatt ctacgatttt gatggcgaca atactcctat tatccgtggt tctgctcttg
      721 gtgcacttaa cggtgtagct caatgggaag acaaagtaat ggaattgatg gatgctgttg
      781 attcatggat tgaacttcct ccacgtgcta ttgacaaacc tttcctaatg ccagttgaag
      841 acgtattctc aattactgga cgtggtacag tagctacagg tcgtatcgaa acaggtgtta
      901 tcaaagtagg agaagaaatt caaattattg gtcttggtgc aggtgcaaaa aaatctgttg
      961 taacaggagt tgaaatgttc cgcaaattat tggatcaagg tgaagctgga gacaacgtag
     1021 gtctattgct tcgtggtgta gacaaagaag aaattaaacg tggtatggtt ataactcacc
     1081 caggtaaagt tactcctcat acttctttca aagcttctgt ttatatcatg aaaaaagaag
     1141 aaggaggccg tcacactcca ttccacaaca aataccgtcc tcaattcttc atccgtacgt
     1201 tagacgttac aggcgaaatc acattgccag atggtgttga aatggttatg cctggtgaca
     1261 acttagaaat tacagttaac ttaatttacc cagttgcttg tagcgtaggt ttacgttttg
     1321 caattcgcga aggtggtaga actgtaggtt caggacagat tactgaaatt attgcataat
     1381 aatcatcatt tagtagttgc tgtaaaaggt aactactaaa tatacacggg tctagctcag
     1441 ttggtagagc actggtctcc aaaaccaggt gtcgggagtt cgagtctctc gacccgtgca
     1501 aattaagaaa tcttatgaag ataacaaatt atatcaaaga atcttataac gaactcgttc
     1561 ataaagtatc atggccaacc accaaagaat tgactaatag tgcaattgtt gtattaattg
     1621 cttccattat tattgcatta atagtttggg tgattgactt tggctttgaa aaattcattc
     1681 agtttatcta caaattgata ggctaattta cggaggaaac aaaatgtctg aaatggataa
     1741 aaaatggtat gtattacgtg ccattagcgg taaagaaaat aaggtaagag agtatattga
     1801 gtcagaaatg aaaaatacaa ctcttagcca atatgtattt caggttttaa ttcctactga
     1861 aaaagtgtat caaattcgta acggaaaaaa aaccatcaaa gaaagaagtt atttgcctgg
     1921 ttatgtcctt gttgaagcaa cactgacggg agaagttgtt caccaattga aaaacttacc
     1981 aaacgttatt ggctttttgg gggataaagc aggtgatcct attcctttgc gtcaatctga
     2041 agtaaatcgt attttgggta cggtagattt gttgcaacaa agtgaagaag aaattaatat
     2101 tccttattat gttggtgaaa atgtgaaagt aatatctgga ccatttaacg atttttctgg
     2161 cgtaatcgaa gaagttaaca atgaaaagaa aaagctcaaa gtaatggtct taatttttgg
     2221 tcgtaaaaca ccattagaat taagctttat gcaagtagaa aaagaacaat aaggttacgc
     2281 tgttgttgct ttttcaaata ttttctaaat taaaaaaatg aattaatatg gctaaagaag
     2341 ttgctggact tattaagttg caagtaaaag gtggcgctgc caacccttct ccccctattg
     2401 gacctgcatt aggtgcgaaa gggattaata ttatggagtt ttgcaaacaa tttaatgcca
     2461 ggacccaaga caaggcaggt aaaatattac ctgttgtaat tacctattat tcagacaaat
     2521 catttgattt tatcatcaaa actcctcctg tagccatcca gttacttgag gtgtcgaagt
     2581 taaaaggtgg ctcagcagag cctaaccgta aaaagctagc tgaaattact tgggatcaag
     2641 taaaggctat tgctactgat aaaatggaag atttgaactg ttttactatt gactctgcaa
     2701 tgagaatggt agcaggtaca gcgagaagta tgggtataac cataaaaggt gaattcccta
     2761 gtagtaatta atctcttcaa ttaaaaacat gagtaaacta acaaaaaatc aaaagttggc
     2821 tttggaaaag attgaagtag gtaaaaacta ctctcttaaa gaagctgcta gcttggtaaa
     2881 agacgttacc aacaccaaat ttgatgcatc tgtagatata gatgttcgtt tgggtgtaga
     2941 tcctcgaaaa gcaaatcaaa tggttagagg tgttgtttct cttcctcacg gaacaggaaa
     3001 aacagtaagg gttcttgtat tatgtactcc cgatgcagaa gcagaagcaa aagaagctgg
     3061 tgcagaatat gtcggtcttg acgaatacat tgaaaaaatt aaaggtgggt ggacagatat
     3121 tgatgttatc atcactatgc cttctatcat gggtaaaatt ggagctttag gtcgggtttt
     3181 aggtcctcgt ggtttaatgc caaatcccaa aagtggaaca gtaaccaatg aagttggtaa
     3241 agccgttaaa gaaattaagc aagggaaaat agattttaaa gttgataaat ttggtattgt
     3301 acacacttct gtaggaaagg tttcttttac tgcagataaa ctagtagaca atgcaaaaga
     3361 atttatttca acgcttaata aacttaaacc tactgccgct aaaggtgtat atattaaaag
     3421 catttatctt tcaagtacaa tgagcccggg tattaaaatt gaccctaaat cagtggaata
     3481 attaaacgat tagaagaagt atgaaaaagg aagataaaag ccaaattata gcacaaatta
     3541 aatctactat tgaagcatat aattgtttct atgttacaga tatagccgat ttaaactcgg
     3601 aaaaaacaag taacttaaga aaactatgtt ttaatcaaaa cattaaattg cttgtagtaa
     3661 aaaatacgtt gttcaaaaaa gcccttgaag atatgggagg agatttttct cctttatttg
     3721 gaactctcaa agggtctacc tctattatgt tttcaaacaa cagtaattct cctgctaaac
     3781 ttatcaaaga atttaaaaaa gataagaaaa acaatggaaa acctgttttg aaagcagcat
     3841 atgttcaaga atgtttttat gtgggagaag accaactaga gtcgttaatc acacttaagt
     3901 caaaagaaca acttcttgga gaaatcgttg gattgttgca atctcctgcc aaaaatgtta
     3961 tttcagctct taaatcagga ggaaatacat tacatggagt gttggaaaca ttatctaaca
     4021 aataacagaa taaaagaatt taattacgaa cttaatttaa aacataagta acatggcaga
     4081 tttgaaagtt tttgcagagc aattagttaa tttgactgta aaagaagtaa acgagttagc
     4141 tcaaatttta aaagatgaat atggtatcga accagctgca gctgcagtag cagtagcagg
     4201 tccagcagta gccgcagaag cagctccagc agaggaagaa aaaagttcat ttgatgtaat
     4261 tttaaaatca gctggtgcaa acaaactagc gattgtaaaa ttagtaaaag aacttacagg
     4321 tttaggcttg aaagaagcta aagatatcgt agatgctgca ccatctgcag tgaaagaagg
     4381 tgtaggtaaa gacgaagctg aaggtttgaa aaaacaattg actgaagcag gagctgaagt
     4441 tgaattgaaa tagtaatatc ctggtaatca ggatatatgg tttagagcct ttattaaaat
     4501 aggttctaaa cctttttatg tttatgtata aaacatgttc gattaaccac ttcgttgata
     4561 gatgtcatac cctatgagta accaaagaat aaacttttca tccattaaaa aatcactgga
     4621 ttatccagat tttttagaag tacaattaaa gtcgtttaaa gactttttcc aacttgatac
     4681 acctcctgaa aaaagaaaaa atgatggatt atatcgagtt tttgcagaaa actttcccat
     4741 tacagataca agaaataatt ttaatttaga atttctagat tactatattg atcctcctcg
     4801 ttattcaata caagaatgta tggaaagagg tttaacgtat agtgtccctt taaaagccaa
     4861 attaaaatta tattgtaccg accctgacca cgaagatttt gatacagtaa tccaagatgt
     4921 ttatcttgga cctattccat atatgaccga aaaaggaact tttgttatca atggagcaga
     4981 gcgtgtagta gtatcacagt tacaccgttc acctggtgta ttttttgggc aaagtttaca
     5041 cacaaacgga acaaaattat attcagcacg tattatccca tttaaaggtt cgtggattga
     5101 atttacaaca gatatcaata acgtaatgta tgcgtatatt gacagaaaaa agaaacttcc
     5161 tgtaacaaca ttgctacgtg ccattggttt tgaaagcgac aaagatatac tagaaatttt
     5221 taatcttgca gaagaaatta aagcaactaa aaccaacctt aaaaaagctg ttgggagaaa
     5281 actagctgca agagtattaa aaacatggat tgaagatttt gtagacgaag atacgggcga
     5341 ggttgtttct attgaaagaa acgaagttat cattgatcgt gaaacaatac tagacaacga
     5401 ccatgtaaac gagatattag aagccaatgt acaaacagta cttttgcata gagaggactt
     5461 aagcagttca gactatagta ttatatacaa cacattacaa aaggatccaa gcaactctaa
     5521 caaagaggct attttatata tttacagaca acttcgtaac gccgaaccac cagatgatgc
     5581 aagtgctaga gaagttattt ataatctgtt tttttcggaa aaaagatatg atttagggga
     5641 tgttggtcgt tttagaatta acaagaaatt agatttagat atctctcctg aaattaaagt
     5701 attaacaaaa gaagatatta ttgccattat ccgttatctc attgaattaa taaattctaa
     5761 agctgacgta gatgacattg accatttaag taatcgtcgt gtacgtacgg ttggtgaaca
     5821 attatgcaat caattcagtg taggacttgc acgtatggca cgtactattc gtgagcggat
     5881 gaatgtacgc gacaatgaag tatttacgcc aattgattta atcaatgcga aagcaatttc
     5941 atctgtaatc aattcatttt ttggcaccaa tgcattgtct caatttatgg atcaaacgaa
     6001 tccgcttgca gaaatgacac ataaacgtcg tatgtctgca cttggaccag gaggactttc
     6061 acgagaaaga gctggttttg aggtgcgtga cgttcactat acgcattatg gacgtttgtg
     6121 tccaatcgaa acgccagaag gtccaaatat tggattgatc tcttctcttt gtgtatatgc
     6181 taaaatcaat gatttaggtt ttatagaaac gccataccgt aaagttgacg acaaaaaagt
     6241 aaatttaaac aaagaggata ttatttatct ttctgcggaa gaagaagaaa atattattat
     6301 cgcacagggt aatgctccac ttgatgataa tggaaacttc acacgtacac gtgttaaagc
     6361 tcgtcaggat agtgatttcc caattgttgc tccagaagaa gtccaattaa tggatgtatc
     6421 tcctactcaa atagcatcaa ttgcagcttc acttattcca tttttggaac atgatgatgc
     6481 aaaccgtgcg ttaatggggt caaacatgat gcgtcaagca gttccattgc tacgttgtga
     6541 agctcctatt gtaggaacag gattggaagg tcaaattata aaagattcaa gaactcaaat
     6601 tacggcagaa agagatggtg taattgagta tgtagatgct acaactataa aagtacgtta
     6661 caattactca gaagaagaat tattcgttca atttgaaagt tctgtaaaag aatacacatt
     6721 accaaaattt caaaagacaa accaaagtac tacaattgat cttagaccga ttgttcataa
     6781 aggagaaaaa gtagttgctg gacagatatt aacagaagga tattcaactc aacaaggtga
     6841 attagcaata gggaaaaact taaaagtggc atttatgcct tggaaaggat ataactttga
     6901 agatgcaatt gttattaacg aacgtattgt acgcgaagat gtatttactt ctattcacgt
     6961 ggatgagtac tctttagaag taagagaaac aaaacgagga ttagaagagc tcactgcaga
     7021 tattcccaac gtaagcgaag atgctacaaa aaatttagat gaaaatggta ttgttcgtat
     7081 tggagcttta attgaacctg gagatatttt aattggtaaa attactccta aaggagaatc
     7141 tgacccatcc ccggaagaaa aattacttcg tgctattttt ggagacaaag ctggagatgt
     7201 taaagatgct tctttaaagg ctcatccatc attaaggggt gttgttattg agaaaagatt
     7261 attttcaaaa gcacaaaaag caaaaaaatc caaatcagca gataaagcca ttatggacaa
     7321 aattgaagct gaatttgaag aaaaagcaca tgcattgaaa ttaaaattag ttgaaaaact
     7381 tttagtatta acaaatggga aaacctctca gggggtaaaa gactttttgg gagctgaagt
     7441 aatttctaaa ggtagtaaat ttactcaaaa acaattgtta gatgtagact actctactgt
     7501 acaaataagc aaatggacaa gtgatagtgc aaaaaatgaa caaatcaaaa cacttatcat
     7561 gaactatttg aaaaaataca aagagtatga tgcccaattg aagagagaaa aatataatat
     7621 tacaattggg gatgaactac ctgcaggtat tattcaatta gccaaagtat atatagcgaa
     7681 aaaacgtaaa gtacgggttg gtgataaaat ggcaggaaga catggtaata aaggtatcgt
     7741 gtctcgtatt gtacgtcaag aagatatgcc atttcttgaa gatggaagcc ctgtggatat
     7801 agtactgaac cctttgggtg tgccttcgcg tatgaactta ggtcaaattt ttgaaaccgt
     7861 attgggatgg gctggaaaag aattaaatgt aaaattttct acgccaattt ttgatggtgc
     7921 tacattggaa gatttagacg aatggactac aaaagcaaac attcctcgtt atggta
//
LOCUS       DAOSRP010000094         7777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_8820_length_7777_cov_5.268195, whole genome shotgun sequence.
ACCESSION   DAOSRP010000094 DAOSRP010000000
VERSION     DAOSRP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8820_length_7777_cov_5.268195"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLJ82_06610"
     CDS             complement(<1..282)
                     /locus_tag="PLJ82_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOR41210.1"
                     /translation="MLNLVIFGAPGSGKGTQSELIAKKYNLVHLSTGDLLRKEIAEQT
                     ELGKLAKGIIDQGKLVSDDVIINLLESNIAKYPNANGFIFDGFPRTVVQA"
     gene            complement(320..856)
                     /gene="hpt"
                     /locus_tag="PLJ82_06615"
     CDS             complement(320..856)
                     /gene="hpt"
                     /locus_tag="PLJ82_06615"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOR41211.1"
                     /translation="MDIVQIKDKRFGLTISEKEILNAIESVAQRINADLQDKNPLFIC
                     VLNGAFMFAADLMKFVTIPSEVTFVKLSSYEGLMSTGKVKEVFGLTENLSGRTVVIVE
                     DIIDTGKTMANLIESLGSKNVAEVYVATLLLKPEALEHTVPLDYVAIEIPNDFIVGYG
                     LDYDGYGRNLRAIYTLIP"
     gene            complement(975..1460)
                     /locus_tag="PLJ82_06620"
     CDS             complement(975..1460)
                     /locus_tag="PLJ82_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOR41212.1"
                     /translation="MKKKLRSISFYLLLWMLSSCVTEPSSSSIPYAWVSFEIDVSTGG
                     VHSSLRRDMLGNMLIFSVSNPSILSSSSNAGYGFGGVIVVRGMDNELYAFDATCPHEH
                     TSDVVLASDGYFVVCPLCKTSYDVASGWGMPNTSSGPGKERLKKYSVYKISENRYRVV
                     N"
     gene            1613..3043
                     /locus_tag="PLJ82_06625"
     CDS             1613..3043
                     /locus_tag="PLJ82_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR41213.1"
                     /translation="MKKLFVTLLSTTILGSLFIGCSTKASTQDNRDCTNIVQPDWSRN
                     AVIYEVNIRQFTEEGTFKAFQSHLPRLKELGVDILWLMPVHPVSEVNRKGELGSYYAV
                     QDYKAVNPEFGTLEDFKSLVDEAHQLGMKVILDWVANHTGCDNVWLQSHPEWYKRDSI
                     GNFIAPYDWTDVYELDYTKNEVREAMADALEFWVKDYNIDGYRCDVAFLVPLDFWEMA
                     RKRLDAIKPVFMLAEAEKPELTVNAFNMVYNWPLKDVMNQIAKGKNAEKANYIHESLL
                     SNASPIKNAQDIDTLVALQDSVFPCDAYQMNFITNHDENSWDGTEHERMGKGANTFAV
                     LTYTLNGMPLIYTGQEVGFDRRFEFFKKDTPPNWQDNETVLFYKKLNQLKKSQPALNA
                     GKSGGKFIKYRTSDDTSIYAFSRKLDEKNEVLVILNLTPEDKVCKFTNAPIGNYTNYF
                     TGKKESFDSKNVELSLKAWEYKVLTK"
     gene            3204..4364
                     /locus_tag="PLJ82_06630"
     CDS             3204..4364
                     /locus_tag="PLJ82_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR41214.1"
                     /translation="MKILIVGKSSPYKGGVAAFNERLAMQLMQEGHEVVIYTYSFIYS
                     FLAGSVGDRFNDNDKPIDDIIIEQKINNFNPLKWEKVIAEIKKEQFELIIYGYADIAS
                     TLCISYISNRLKKMAIQVAVMHNVEIDHPFEGIKKMAMTMMIKQLDGFISMSNKVTEE
                     LKPFNKKHRPVIFSPHPIYDLFGKGTDKKLALLQLGLNPNYQYVLFFGAIKESKGLDL
                     LIEAFADERVRQLPIKLLVVGDFNNNKEFYTDIMYENRLKNNIIFVDEYVPNHVVSLY
                     FSACSIVGQPYREVNQSGILQVAYHFDKPILITNIAQCGINIPNQKVGYVINPNTTEI
                     ADSLVDFFVNKREEEMANNVKIEKKKFMWNNMTEAIYSLYQTAKAYKNTRLI"
     gene            4441..5004
                     /gene="efp"
                     /locus_tag="PLJ82_06635"
     CDS             4441..5004
                     /gene="efp"
                     /locus_tag="PLJ82_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR41215.1"
                     /translation="MATTADIRNGMCIEFNNGLYTIVEFLHVKPGKGAAFVRTKMKNV
                     QTGRILENTFNAGVKLDEVRIERRPYQFLYKDDMGYNFMHNETFEQISIDKDKINGVD
                     FLKEGDIAEVTVHADTETILFADLATTTILEVTYTEPGLKGDTATNTLKPATVETGAT
                     VRVPLFINVGDKIKINTTEGSYVERAK"
     gene            complement(5136..5519)
                     /locus_tag="PLJ82_06640"
     CDS             complement(5136..5519)
                     /locus_tag="PLJ82_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41216.1"
                     /translation="MKKKMSVLIVSLMLTHVMLATVHIHYLQKSVYETGRQQWGDWQN
                     EANAFCIDSTILSIVNLSTKSETVYVRQGEAIGGYNERGNYYRLFEAQNEKGQKCLFF
                     LYSDRNVGLLFKCEEAGVIIQYAGY"
     gene            complement(5549..6721)
                     /locus_tag="PLJ82_06645"
     CDS             complement(5549..6721)
                     /locus_tag="PLJ82_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41217.1"
                     /translation="MNWPAESWSSAMNLTSKLTQTNVTELSGLHWNPATQFLYLVQDN
                     GRLRILQFNEGVDDFFEIANVEIAGNPEGIMQVDYEASMFYTVDEKNYEIRQYVPNQD
                     YTSITLMKKWRLLTPNTPMPDTGNDGIEGIVFVPDKHLQSIGFVSSQTGLPYTSTKGM
                     GGLIFIAHQTEGYVWVYDVNPTLSFDVAYVGKYKTNRKESCDIAFDRTTGLLYILHNV
                     GKNFLEVTNLSLEQVNDEYKFVVSKEYSLSNPTDGNENIEGFAITPMCEENGVVSVFV
                     CRDVNDTGTEIQKGDALRWFTDFPLLGSCFPITEDEQIQTDNARFTIEFTSLNGLYIH
                     GAQENVELVFMDELGRIRYKTHYEPMSYLPYDVLPQGYSILQIHTSNDIQVVLIRL"
     gene            7037..7642
                     /locus_tag="PLJ82_06650"
     CDS             7037..7642
                     /locus_tag="PLJ82_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOR41218.1"
                     /translation="MKKVMLLVLGFLLGLNIIAQNEKPFYLGLYASPSVLWLQSDNNT
                     VDASGSKLGFSYGLLADITLGDYYALASGLEITQLGGTLSDQTVDYKLQYLEIPLALK
                     MQTASINNLSFFGRFGGGFGVRLDAKKDVNNTIINQPASDEIHLLRAYFSVGGGVEYT
                     VAYNTKIFAGIFYKNGLTNAFKQEDWSVKSNALSLNVGVIF"
BASE COUNT         2763 a         1344 c         1240 g         2430 t
ORIGIN      
        1 tgcttgtact actgtgcgag gaaaaccatc gaaaataaag ccatttgcat tcggatattt
       61 agctatattg ctttctaaga ggttaataat tacatcatca gacactaatt taccttgatc
      121 aataatacct tttgccaatt taccaagctc tgtttgttca gcaatttctt tgcgtaacaa
      181 atcccctgtt gaaaggtgta ctaaattata tttctttgca attaactcac tctgagtacc
      241 tttgcctgag ccaggagctc caaaaataac gagatttaac atattctttt tcttatttat
      301 aaaattatat atagtttgtt tatggtatta aggtgtaaat ggcacgtaaa tttcgtccgt
      361 aaccatcgta atctaagcca tagccgacga taaaatcatt gggtatttca atagccacat
      421 aatctaatgg cactgtatgt tccaaagctt ctggtttcaa cagcaacgtg gcaacatata
      481 cttcagcaac gtttttcgag cctaaagatt ctatcaaatt agccattgtt ttgcctgtgt
      541 caatgatatc ttctacaatc acaacggttc ttcctgataa attttctgtc aagccaaaca
      601 cttcttttac ttttccagtt gacattagcc cttcgtacga tgataactta acaaaagtaa
      661 cttctgatgg aatagtaaca aatttcatca aatctgcggc aaacataaaa gccccattca
      721 acacacatat aaataatgga tttttgtctt gtaaatcggc attaatacgt tgagcaacgc
      781 tttctattgc atttaaaatt tctttttcag aaatagttaa accaaatctt ttatctttta
      841 tctgtacaat atccatacta agtgtttaaa aaaactgcac gaagttaatg aaaaaactaa
      901 taacctgaaa aaaaatagca ataacacgct gaaatattta tcactgaata taagttatac
      961 tccaccaaaa gtagctaatt tactactcta tacctatttt ctgaaatttt ataaacactg
     1021 tattttttta agcgttcttt tcctggtcct gagcttgtgt taggcattcc ccagccagaa
     1081 gcaacatcat aagaggtttt acacaatgga cataccacaa aataaccatc acttgccaaa
     1141 acaacatcag atgtgtgttc gtgaggacat gtagcatcaa atgcatataa ttcattatcc
     1201 atacctctta caactattac acccccaaaa ccataaccag cattgcttga tgaagacaat
     1261 attgaaggat ttgatacaga aaaaataagc atattaccaa gcatatctct acgtaaagaa
     1321 ctatgaacac ctcctgtaga aacatctatt tcaaaagaaa cccacgcata cgggatactt
     1381 gaactagaag gctctgttac acaagaagac agcatccata gtaatagata aaatgaaata
     1441 cttcgcaatt tttttttcat aacatgcaaa gatacatacg tttacatata aaacttatat
     1501 gtccgtataa aaaacaatga aaaattagat attttttggt ttcgatataa atatatcgca
     1561 tatttgtaac acaattgtca ttaatattaa ctaatcaaat gaatgtctac ttatgaaaaa
     1621 attatttgta acgttgttat ccaccacaat tttgggtagc ctatttattg gatgctctac
     1681 caaagcatct acgcaagata accgagactg taccaatatc gtgcagccag actggtcgag
     1741 aaatgctgta atttacgaag taaatatccg tcagtttaca gaagaaggta catttaaagc
     1801 ttttcaatct catttaccac gtttaaaaga attaggggtg gatattttgt ggcttatgcc
     1861 cgtgcatcca gtatctgaag taaacagaaa gggagaatta ggaagttact atgctgtaca
     1921 agattacaaa gctgtcaatc ctgaatttgg cacattagaa gattttaagt cgttggtcga
     1981 cgaagctcat caattgggaa tgaaagtaat tcttgactgg gtagcaaatc atactgggtg
     2041 tgataatgtt tggttacaat ctcatccaga atggtacaag cgagactcta taggtaattt
     2101 tatagctcca tacgattgga ctgacgtata tgaattagac tataccaaaa atgaagtgag
     2161 agaagcaatg gctgatgcat tagaattttg ggtcaaagat tacaatatag atggctatcg
     2221 ctgtgatgta gcgtttttgg ttcctcttga tttttgggaa atggctcgta aaagacttga
     2281 tgctattaaa ccagttttta tgctagccga agcagaaaaa cccgaactaa ccgtgaatgc
     2341 atttaatatg gtatataact ggccacttaa agatgttatg aaccaaatag ccaagggtaa
     2401 aaatgctgaa aaagcaaact acatacacga aagcttatta agcaatgcaa gtccaattaa
     2461 aaatgctcaa gacattgata cacttgtggc tcttcaggac tctgtttttc cttgtgatgc
     2521 atatcaaatg aattttatta ccaaccatga tgaaaattcg tgggatggaa ctgaacatga
     2581 acgcatgggt aaaggtgcga acacatttgc tgtattaacc tatacattaa atggaatgcc
     2641 tcttatttat acaggccaag aagtgggttt cgatcgtcgt tttgaatttt tcaaaaaaga
     2701 tactcctccc aattggcaag ataacgaaac tgtactattc tacaaaaagc taaatcaact
     2761 gaaaaaatct caaccggctt taaatgcagg gaaaagtgga ggcaagttta taaaatatag
     2821 aacttcagac gatactagta tttacgcctt ttcacgcaaa ttagatgaaa aaaatgaggt
     2881 acttgtcatt ctaaatctaa ctcccgaaga taaagtatgt aaatttacca acgctcctat
     2941 tggtaactat acaaattatt ttactggcaa aaaagaatca tttgattcaa aaaatgttga
     3001 gttatctctc aaagcctggg aatacaaagt gttaacaaaa taaagaacat aaaatcattc
     3061 aaacacatta aaacgaagaa tcttgtcgaa aaacaaaggt tcttcgtttt aattttggta
     3121 aagttaaatg aaagtagcat atttgcaacc tgaaatgaca caaactagtt atttagaaaa
     3181 atctaataac ctaacttacg accatgaaga ttcttattgt agggaaaagt tctccgtata
     3241 aggggggtgt ggcagccttt aacgaacgtt tggcaatgca actcatgcaa gaaggacacg
     3301 aagtggtaat ctatacctat tccttcattt attcattttt agcaggtagc gtaggcgacc
     3361 gctttaatga caacgataaa cccattgatg atattattat tgaacaaaaa atcaataatt
     3421 ttaatccatt gaaatgggaa aaagtcattg cagaaataaa aaaggagcaa tttgaactca
     3481 ttatatatgg ctacgctgat atagcttcta ctctttgtat aagctatata tccaatcgtt
     3541 taaagaaaat ggctatacaa gtagctgtaa tgcacaacgt agaaattgat catccatttg
     3601 aaggtataaa aaaaatggct atgactatga tgattaaaca attagatggg tttatttcta
     3661 tgtcaaataa agtaaccgaa gaacttaaac cttttaataa aaaacatcgc ccagtaattt
     3721 tttctcctca ccctatttat gacctttttg gcaagggtac tgacaaaaaa ttagctcttc
     3781 tgcaacttgg tttaaatcca aattatcaat atgtcttatt ttttggagct atcaaagaaa
     3841 gtaaagggtt ggatttactt attgaagctt ttgcagatga acgggtacgt caattaccta
     3901 taaagctttt ggtagtggga gattttaaca ataataagga gttttatacc gatatcatgt
     3961 acgaaaatag actcaaaaat aatatcattt ttgtagacga atatgttcct aatcatgttg
     4021 ttagtctcta tttttcagca tgtagcattg tcggacaacc ctatagagaa gtaaatcaaa
     4081 gcggaatttt acaagtagcc taccactttg acaaacccat tctaattaca aatattgctc
     4141 aatgtggcat taatattcct aatcaaaaag tgggctatgt gattaatcca aatactacag
     4201 aaatagcgga ttcgttagta gatttttttg tcaataaacg cgaagaagaa atggcaaaca
     4261 atgtaaaaat agagaaaaag aaatttatgt ggaacaatat gactgaagcc atttacagtt
     4321 tatatcaaac agctaaggct tataaaaata ctcgtttgat ataatttttt ttgatgttat
     4381 agaacaaata actatttttg caccctaatt attagcaaat tatatactta aattattcca
     4441 atggcaacta cagcagacat aagaaatggt atgtgtattg aatttaacaa tggactttac
     4501 acaattgtag aatttttaca cgtaaaacca ggtaaaggtg cagcatttgt acgcacaaaa
     4561 atgaaaaatg ttcaaacagg tcgtattctc gaaaatacat ttaatgcagg cgtaaaactt
     4621 gatgaagtac gtatcgaacg tcgtccatat caatttttat acaaagatga tatgggatac
     4681 aactttatgc acaacgaaac atttgaacaa atatcaattg ataaagacaa aatcaatgga
     4741 gtggacttct tgaaagaagg agatattgca gaagtaactg tacatgctga tacagaaaca
     4801 atattatttg ccgaccttgc taccaccacc atcctagaag taacatacac tgaaccagga
     4861 ttaaaaggag atactgcaac caacacacta aaacctgcta cagtagaaac aggtgctacg
     4921 gttcgtgttc ctttgtttat caatgttggg gataaaatta agataaatac aacagaagga
     4981 tcgtatgtag aacgtgcaaa ataactttcc ctatgagata ttaaaagagg ctggctcaaa
     5041 ataaaatgag ccagcctctt ttaatagtat aatgaaagta acaagaaata tgtagtaaat
     5101 atacttcaaa caactataat acctgattgt attcactaat atcccgcata ttgtatgatg
     5161 acacctgctt cttcgcactt gaataataag cctacatttc ggtctgaata caaaaaaaac
     5221 agacatttct gtcctttttc attttgagct tcaaataaac gatagtagtt tcctcgttca
     5281 ttatatccac ctattgcttc accttgacga acatatactg tttctgactt agtagaaagg
     5341 ttaacaatac tgagaattgt tgaatcaata caaaatgcat tggcttcatt ttgccaatca
     5401 ccccattgtt gcctaccggt ttcatagaca gatttttgta gataatgaat atgaacagtt
     5461 gccaacatta catgagtcaa cataagacta acaattaata cactcatttt ctttttcata
     5521 tatatcattt ttataattaa acaaacaact acaatcgaat caaaacaact tgaatatcat
     5581 ttgatgtatg tatttgcaat atactatacc cctggggtaa tacatcatat ggcaaataag
     5641 acataggttc atagtgagtt ttatatcgta ttcttcctaa ttcatccata aaaactaact
     5701 ctacattttc ttgggcacca tgtatgtata agccatttaa tgaagtaaat tcaatagtga
     5761 atctcgcatt atctgtttga atttgctcat cttctgttat tggaaaacaa cttcctaata
     5821 aaggaaaatc cgtaaaccat cgtaaggcat ctcccttttg gatttctgta cctgtatcat
     5881 tcacatctcg acatacaaac accgaaacca ctccattttc ttcacacatg ggagtaattg
     5941 caaaaccttc aatattttca ttcccatccg tggggttcga cagtgaatat tcctttgata
     6001 cgacaaattt atactcatcg tttacctgtt caagagataa attggtaact tccaaaaaat
     6061 tcttccctac attatgtaat atatataaca atccggtggt acgatcaaaa gcaatatcac
     6121 aactctcctt acggtttgtt ttgtatttgc ccacatacgc tacatcaaaa gacaaggttg
     6181 gattaacatc atatacccat acataacctt ccgtttgatg tgcaataaaa attaatccgc
     6241 ccataccttt agtagaagtg tacggcaagc ctgtttgaga gcttacaaat ccaatcgatt
     6301 gcaaatgctt atctggcaca aatacaattc cttctatacc atcgtttcca gtatcaggca
     6361 tgggcgtatt gggggttaac aatcgccatt ttttcattaa agtaatagaa gtataatctt
     6421 gattgggaac atattgtcgt atttcataat ttttttcatc aactgtataa aacatacttg
     6481 cctcataatc tacctgcatt atcccttcag gatttcctgc tatttcaaca ttagcaatct
     6541 caaaaaaatc atcaactcct tcgttgaatt gaagaatccg taagcgtcca ttatcttgta
     6601 ctagatacaa aaattgtgta gcaggattcc aatgtaagcc actcaactct gttacattcg
     6661 tttgtgttaa cttagatgtt aaattcatcg cactactcca tgactcagct ggccaattca
     6721 acgtttgaga aaacaaagga aatggtaaaa taagtatgtg cattaaggcg tagaaaacaa
     6781 ctttatggga ttgtctatat tgaggcaagg ataacatatc agattaattg aataagaata
     6841 gttaagataa tagcaaagat aagatataaa tatagaataa tccaagaaaa aaaatattcg
     6901 cctaataaag caagatatat cctatctaaa tagctttaaa aaaaatataa aaaaaagacc
     6961 acttatcctt gtattactgt attttttagt atatttgtgt attgataatt attgttttca
     7021 acaaaaagat acagatatga aaaaagtaat gctacttgta ctaggcttct tattaggatt
     7081 aaatatcata gctcaaaacg aaaaaccatt ttatttaggg ctatatgcat ccccttctgt
     7141 tttatggtta caatcagata ataacacggt ggatgctagt gggtcaaagc tcgggttttc
     7201 atacggattg cttgcagata tcaccttagg agattattat gcacttgcta gtggtttaga
     7261 aataactcaa ctaggaggaa ctctttcgga ccaaacagtt gattacaaat tacaatattt
     7321 ggaaataccc ttagccctaa aaatgcaaac tgcatccatc aataatttaa gtttttttgg
     7381 tcgttttggt ggtgggtttg gagtacgtct agatgccaaa aaagatgtaa ataacaccat
     7441 tatcaaccaa cctgcaagtg atgaaataca tctcttacgt gcctatttta gcgttggtgg
     7501 aggtgtagaa tatactgttg catacaacac taaaattttc gcaggtattt tctacaaaaa
     7561 tggattaaca aatgctttta aacaagaaga ttggagtgta aaaagcaatg ccttatcatt
     7621 gaatgttggt gttatttttt aacgaactct ttattataaa atggatacac ataagaacgg
     7681 tttaactaaa ttccgttctt atttatttgt agatgtgaaa aaagtaggta aacataaaaa
     7741 aagtccttct ttcaaaggac tttcgcggaa gcggggg
//
LOCUS       DAOSRP010000095         7457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9271_length_7457_cov_5.293434, whole genome shotgun sequence.
ACCESSION   DAOSRP010000095 DAOSRP010000000
VERSION     DAOSRP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9271_length_7457_cov_5.293434"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..715
                     /locus_tag="PLJ82_06655"
     CDS             173..715
                     /locus_tag="PLJ82_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOR41219.1"
                     /translation="MTYVFLANGFEEIEAISTIDILRRAGIETTTVSIEPQLAVIGAH
                     NIVVVADQLFAEGAYNNAECLLLPGGMPGTSNLNKHEKLKELISQHVHSGKLMGAICA
                     APLILGELNLLQGKIATCYPSFETYLHGATISDDKVADAGEIVTGKGPGFSMDFALTI
                     VKKLKGACAADEVAKGLLLQ"
     gene            complement(808..2652)
                     /gene="glmS"
                     /locus_tag="PLJ82_06660"
     CDS             complement(808..2652)
                     /gene="glmS"
                     /locus_tag="PLJ82_06660"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOR41220.1"
                     /translation="MCGIVGYIGKQDAYPILIKGLHRLEYRGYDSAGVALINEAGSLN
                     VYKSKGKVSDLEQSVTSKDISGTIGVAHTRWATHGEPNNENAHPHYSQSESLALIHNG
                     IIENYMVIKKELIRNGYTFKSSTDTEVLVQFIEYVKKTNQVDLCHAVQIALREVVGAY
                     AIAVLDKNDPKQIIAARKSSPLVVGIGDGEFFLASDATPIIEYTNKVVYVNDEEIAVI
                     HIDKGLKLLNLVDIEQTAEVKQLEMNLSQLEKGGYEHFMIKEINEQPHTLIDCIRGRI
                     NVEGTDVKLSGVIDHQDKFLKAKRIIIVACGTSWHAGLIGEYLIEDLCRIPVEVEYAS
                     EFRYRNPIIHPDDIVIAISQSGETADTLAAVEIAKSQGAFIYGVCNVVGSSIPRATHS
                     GSYIHVGPEIGVASTKAFTGQITVLSMLALTVAKLRKTIQPEEYRKLIGELQAIPNKM
                     QEVLKLDNQIKDLAKMFTYAHNFIYLGRGYNYPAALEGALKLKEISYIHAEGYPAAEM
                     KHGPIALIDAEMPVVAIATKDSIYDKTVSNIQEIKARKGRVIAVVTEGDDVVSEIADY
                     CIAIPESLEYFAPIVSAIPLQLFAYYIAVNKGRNVDQPRNLAKSVTVE"
     gene            3099..3641
                     /locus_tag="PLJ82_06665"
     CDS             3099..3641
                     /locus_tag="PLJ82_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41221.1"
                     /translation="MSKLIKFTKMHGAGNDYVYINGFEETITSPSAFSIRISDRHFGI
                     GSDGLVAVTAGCDVVSGWGAVAIGLLAGILLVFSLRLIENVFKIDDPVGAASVHGITG
                     AFGTLAVGLFSQSTGLFYGFGTQALLSQFIGVVSVAAWGLGTGFILFFILKKTVGLRI
                     DKRVEEEGLDIYEHGETAYN"
     gene            3732..4766
                     /gene="asnA"
                     /locus_tag="PLJ82_06670"
     CDS             3732..4766
                     /gene="asnA"
                     /locus_tag="PLJ82_06670"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOR41222.1"
                     /translation="MLLYIPKNYTPSLDSEKMELAIKQLKDDFQAELTHQLNLRRVTA
                     PLFVLSGTGVNDDLNGTERAVSFPIKDMGNRRAEVVHSLAKWKRMKLGAYNIPAGYGL
                     YTDMNAIRADEELDNLHSLYVDQWDWEQTITQEDRTIEFLKSTVKKIYQALKTIELKL
                     HGKYPHISPTLPSEIRFFHSEELLSLYPELSPRERETKVAEKYGAVFVIGIGAPLADG
                     KKHDGRAPDYDDWSTPTSKGLRGLNGDIILWNKVLDSTFEISSMGIRVDKKALLYQLK
                     EEGCMNRTELDFHSQLINEKIPLSIGGGIGQSRLCMYFLKCAHIGEVQASLWPEEMIE
                     SCKANKILLK"
     gene            4786..6975
                     /locus_tag="PLJ82_06675"
     CDS             4786..6975
                     /locus_tag="PLJ82_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004315725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOR41223.1"
                     /translation="MNTLRFKVLDEAFRRKAVQLPESKERLTDYYAKYVFDRNKMQRY
                     LSKNTYGILTNSIDKGEPLNRSIADGVATGMKQWAMEMGATHYTHWFQPLTGGTAEKH
                     DSFLDFNNIGGVIEDFSGKLLAQQEPDASSFPSGGIRNTFEARGYTAWDPSSPAFVVG
                     DTLCIPTIFISYTGEALDYKTPLLKSIGVLNKAAVDVCKYFDENVSKVTSFLGWEQEY
                     FLVDEALWAARPDLMLTGRTLMGHSSAKNQQLEDHYFGAIPSRVLAFMKDLEIESLKL
                     GIPAKTRHNEVAPNQFELAPIYEESNLANDHNLLLMSVMKKIARRHNFRILFHEKPFK
                     GINGSGKHNNWSIGTDTGVNLLSPGKTGIENLQFITFMVNVLMAVYKNNALLKASISS
                     ATNAHRLGANEAPPAVVSVFLGKQITNFLDKMEVASTDEAIIFDDKVGIRLGVAHIPE
                     ILLDNTDRNRTSPFAFTGNRFEFRAVGSSANCGGAMMALNTAVAGQLKQFKTEVDALI
                     ASGMLKEKAIVTVLKKYITISKPIRFDGNGYSDEWKIEAAKRGLDCETSVPKIFDAYL
                     SKQSIELFKKTNILSEVELHSRCEVKWETYTKKIQIEARVLGDLAMNHILPLASRYQS
                     LLLDNVSKISTIFSADEAKSMTEQDRTIIKTIANHISEIAKQTTELISARKVANKIES
                     EREKAIAYHDTVIPVMDELRSHIDDLEMIVDDEMWTLPKYRELLFIR"
     gene            7081..7457
                     /locus_tag="PLJ82_06680"
                     /pseudo
     CDS             7081..7457
                     /locus_tag="PLJ82_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778299.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2481 a         1318 c         1281 g         2377 t
ORIGIN      
        1 accggtcacc tcgacactgc cagtgtcgcg ctctagccag atgagctaaa cccccttttt
       61 tgcagtgcaa atatacaatc ttttccaaaa taattactat atttgtaaat gtttgttaga
      121 tagcaaacag tatttttcac acaattattc atacatttaa acacaaaata ctatgacgta
      181 cgttttttta gccaatggtt ttgaagaaat agaagcaatt tctaccatcg acatcttacg
      241 cagagcaggt atcgaaacta caactgtttc aatcgaacct caactagctg ttattggagc
      301 tcacaatatt gtggttgttg ctgaccaatt attcgcagaa ggagcatata ataatgctga
      361 gtgtctactg ttaccaggag gtatgcctgg tacttcgaac ttaaataaac atgaaaaact
      421 aaaagaatta atcagccagc atgtacatag tggtaaatta atgggagcca tttgtgctgc
      481 accacttatt ttgggcgagt tgaatttatt acaaggaaaa attgccactt gttacccaag
      541 ttttgaaaca tatttacacg gagctactat aagtgatgat aaagtagcag atgctggcga
      601 aatagttaca ggaaaaggtc caggatttag tatggatttt gctctaacaa tagtaaaaaa
      661 actaaaaggg gcttgtgctg ctgacgaagt agctaaggga ttgttgttgc aatagtttcg
      721 ttacacattg atttttaacc tctatctaac aaaagaatcg ccctattttg gagcgattct
      781 ttttaatata aacaaaataa atagctttta ctctaccgtt accgatttgg ctaaattacg
      841 tggttggtct acattgcgac ccttatttac agctatataa taagcaaata actgcaatgg
      901 aatagccgaa acaataggag caaaatattc taatgattct ggtatagcaa tacagtaatc
      961 agcaatctcg ctaaccacat cgtctccttc tgtaaccacg gcaataaccc ttcctttacg
     1021 ggcttttatt tcttgaatat tgctaacagt tttatcataa atactatctt tagtagctat
     1081 cgccactact ggcatttctg catcaatcaa cgctatagga ccatgtttca tttctgcagc
     1141 aggataccct tcggcatgga tatatgatat ttcttttagt tttaatgctc cctcaagggc
     1201 tgctggataa ttatagcctc gacccaaata tataaaatta tgggcatacg taaacatttt
     1261 tgccaaatct tttatttggt tatctagttt gagtacttct tgcattttat ttggaatagc
     1321 ttgcaattct ccaataagtt ttctgtactc ttctggttga atagttttac gcaatttggc
     1381 tacagtcaac gccaacattg acaatacagt tatttgtcca gtaaatgcct tagtagatgc
     1441 aacaccaatt tctggaccta catgtatata cgatcccgaa tgtgttgcac gaggtataga
     1501 cgatcctacc acattgcaga caccataaat aaatgctcct tgacttttag caatttctac
     1561 agcagctaag gtgtcggcag tttctcctga ttgagaaatg gctattacaa tatcatctgg
     1621 atgaataatt ggattgcgat acctaaattc cgatgcatat tcaacctcta cgggtatacg
     1681 acacaaatct tctatcaaat attcgcctat taatcctgca tgccaagaag taccacatgc
     1741 tacaataata atacgtttag ctttcaaaaa tttatcttga tgatcaatca caccagacaa
     1801 ttttacatca gtaccctcaa cattaattcg tcctctgata caatctatta aggtgtgtgg
     1861 ttgttcgttg atttccttaa tcataaaatg ctcataccca cccttttcaa gttgactaag
     1921 attcatttcc aactgtttta cttcagctgt ttgttcaata tcaactaaat tgagcaactt
     1981 taaaccctta tctatatgta tcacggctat ttcttcgtca ttcacataca ctactttatt
     2041 cgtatattca ataattggag tagcatcaga ggctaaaaaa aactcgccat ccccaatccc
     2101 tacaactaat ggactacttt tgcgagcagc aattatttgc tttggatcat tcttatcaag
     2161 cacagcaata gcatatgcac ctactacttc tctcaacgca atttgtacag catgacacaa
     2221 gtctacttga ttcgtttttt ttacatattc tataaattgt accaatacct cagtatctgt
     2281 agagctctta aatgtataac cattccgaat tagctctttt ttgattacca tataattttc
     2341 gataatccca ttatgaatta atgccagcga ttctgattga gaataatgag gatgggcatt
     2401 ttcattatta ggctcaccat gtgtagccca tcgagtatgt gccactccta tagtaccact
     2461 aatatctttt gaagtaactg attgttctaa atcagatacc tttcctttcg atttatacac
     2521 atttaagcta ccagcttcgt tgattaaagc cacaccagca ctatcataac cacggtattc
     2581 caaacgatgt aagcctttaa ttagtattgg ataagcatct tgcttcccta tatatcctac
     2641 aattccacac ataaattcgt tattttaaaa agtgtatacc ataacatatt ttgctacaaa
     2701 gatatttatt ttctccttat cctattccat attaagcagt tttttattta aaaatttaag
     2761 tgaaaataaa tctttttgta attattatcg aaatatttta atcaatttgc caatttatca
     2821 tccattttgt atcatattca acatattttt tcctattaaa agaatttttt acatacttaa
     2881 ttttagcatt ttattttaac aacaatttca ttatgtgaca ttttaaacgt attttgcttt
     2941 catattaaat tttttatagt taatttagtt tgtttttgat attattgaaa ataaaaatag
     3001 catttataca catttttctt gtctggaaat atcattattc atacttttgc attgaaaaca
     3061 aaacattttg tcattattta atcgatttac aatatattat gtcaaaacta atcaaattta
     3121 cgaagatgca tggagccgga aacgactacg tatatataaa tggttttgaa gaaacaataa
     3181 catctccttc agcattttct atacgtatta gcgatcgtca ttttggaatt ggttcagacg
     3241 gattagtagc tgtaaccgct ggttgcgatg tagtaagtgg atggggtgct gtagctatcg
     3301 gattattagc gggtatatta ttggtattct cacttcgttt gattgaaaac gtattcaaaa
     3361 ttgatgaccc cgttggtgca gcctctgtac atggtattac aggtgcattt ggcacattag
     3421 ctgttggctt attttctcaa tctactggtt tattctatgg atttggaaca caagctctat
     3481 tatcacaatt tattggggta gtttcagtag cagcgtgggg attaggcaca ggatttattc
     3541 tattctttat cttaaagaaa acagtaggtt tacgtattga caaacgagta gaagaagaag
     3601 gtttggatat atatgaacat ggtgaaactg cttataacta attaaacttt tatcaaagag
     3661 ggggctattg aaaaaacttt agtcctctct ttttcaatga tttctaaaac gcaaactaaa
     3721 ttgatgtaac tatgttactt tatattccaa aaaactatac ccctagcctt gattctgaaa
     3781 aaatggaatt ggctattaaa caattgaaag atgattttca agctgaactt acgcatcaac
     3841 taaacctacg tcgtgttacg gctccactgt ttgttttatc tggaactgga gttaatgatg
     3901 atcttaatgg aacagaacgt gcagtatcat ttccaataaa agatatgggc aacagacgtg
     3961 cagaagtagt acattcgtta gccaaatgga aaaggatgaa attaggtgct tacaacatac
     4021 ctgcaggata tggtctttat acagatatga atgccattag agctgatgag gaattggata
     4081 atttacattc attatacgtt gatcaatggg attgggaaca aacaattacc caagaagata
     4141 gaactattga attcttaaaa tcgacagtta aaaagattta tcaagctctc aaaacaattg
     4201 aattgaaact tcacggaaaa tatccacata tttcgcctac attacctagt gaaatacgct
     4261 tttttcattc agaagaatta ttatctttgt acccagaact tagccctaga gaacgagaaa
     4321 caaaagtggc tgaaaaatat ggtgctgttt ttgtaatagg aattggagct ccactagcag
     4381 atggaaaaaa acacgatgga cgagctcctg attacgacga ttggtctact ccaacaagca
     4441 aaggactaag agggttaaat ggagacatca ttttgtggaa taaagtatta gatagtacat
     4501 ttgaaatatc ctctatggga atacgggtag acaaaaaagc actcttgtat caactaaaag
     4561 aagaaggatg catgaatcgg acggaattag attttcatag tcaactaatt aatgaaaaaa
     4621 ttcctttatc tatcggagga ggaataggtc agtctagatt atgtatgtat tttctaaaat
     4681 gtgcacatat tggcgaagtc caagcatcat tatggcccga agaaatgatt gaatcatgta
     4741 aagcaaataa aattctatta aaataataac tattttttta cacgtatgaa cacactaaga
     4801 tttaaagtat tagatgaagc gtttagacgc aaagccgttc agttacctga aagcaaagaa
     4861 cgtttaactg actattatgc caagtatgtg tttgatcgta acaagatgca acgttacctt
     4921 tcaaaaaaca cttatggaat attaactaat tcaatagata aaggtgaacc attaaataga
     4981 agcattgctg atggtgtagc tacaggtatg aaacaatggg caatggaaat gggtgcaact
     5041 cattatactc actggtttca acctcttact ggcggaacag ccgaaaaaca cgattctttc
     5101 ttagatttca ataacatagg tggagtaatc gaagatttct caggaaaatt attagcacaa
     5161 caagaacctg atgcttcaag tttcccaagt ggaggtatcc gtaatacttt cgaagctaga
     5221 ggttatactg catgggatcc atcttctcct gcttttgtcg ttggcgatac actttgtatc
     5281 cctactattt ttatttcgta cacaggtgaa gctcttgact acaaaactcc tctactaaaa
     5341 tcaatcggcg tattgaacaa agctgccgtt gatgtatgca aatattttga tgagaacgtg
     5401 agcaaagtaa ctagcttttt gggttgggaa caagaatact tcttggttga tgaggctttg
     5461 tgggcagcac gtcctgactt aatgcttact ggacgtacct taatgggaca cagtagtgct
     5521 aaaaaccaac aattagaaga ccactacttt ggagcaattc caagtcgcgt gttagctttt
     5581 atgaaagact tagaaattga atcattaaaa ttgggtatcc ctgcaaaaac aagacacaat
     5641 gaggttgctc caaaccaatt tgagttagct ccaatttatg aagaaagcaa cttagcaaac
     5701 gaccacaatt tattgttgat gtcggtaatg aagaaaattg ctcgtcgtca taatttcaga
     5761 attttattcc acgaaaaacc ttttaaaggc attaatggtt caggtaaaca taataactgg
     5821 tctatcggaa ctgataccgg ggttaatttg ctttctccag gtaaaacagg aattgaaaac
     5881 ttgcaattta ttacctttat ggtaaacgta ttaatggctg tttacaaaaa caatgcttta
     5941 ttaaaagctt ctatcagctc ggcaaccaac gctcatcgtt taggtgccaa cgaagctcct
     6001 cctgctgtcg tatctgtatt cctaggaaaa caaattacca atttcttaga caaaatggaa
     6061 gtagcaagta ccgacgaagc tatcatattt gatgacaaag taggtataag attaggtgtg
     6121 gctcatattc ctgaaatttt acttgacaat acagaccgta accgtacttc accttttgca
     6181 tttactggaa accgtttcga gttccgtgca gttggttcat cagctaactg tggtggtgca
     6241 atgatggcat taaatactgc agtagcaggc caacttaaac aatttaaaac cgaagttgat
     6301 gcattaattg caagcggtat gttgaaagaa aaagctattg taactgtatt gaaaaaatac
     6361 ataactattt ctaaaccaat ccgttttgat ggcaacggat atagcgacga atggaaaata
     6421 gaagcagcta aacgtggatt agattgtgaa acaagcgttc caaaaatatt tgatgcatat
     6481 ttaagcaaac aatctattga attattcaaa aagacaaata ttttgagcga agtagaatta
     6541 cactctcgtt gcgaagtaaa atgggaaacc tatactaaaa aaattcaaat cgaagctcgt
     6601 gtactaggtg atttagcgat gaatcatatt ttaccattgg cttctcgtta tcaatcgtta
     6661 ttgttggata atgtatcaaa aatcagtaca attttctctg ctgacgaagc aaaatctatg
     6721 actgaacaag accgtacaat tattaaaaca attgctaacc atattagcga aattgctaaa
     6781 caaacaacag aactaataag tgctcgtaag gttgcaaaca aaattgaatc tgaaagagaa
     6841 aaagcaattg cataccacga taccgtaatt cctgtaatgg acgaactacg ttctcatatt
     6901 gatgatttag aaatgattgt tgatgatgaa atgtggacat tgccaaaata tagagaatta
     6961 ttgtttatcc gctaatttta agcagattat atctgcaaat aaactatttt tatgtcttat
     7021 tttggtcaag aattcttgac caaaacaggc atttacaact cgttttttaa caaaatttat
     7081 atgaaaaacg caacaattat acctgagcag caaggtctgt atgatccaat aaatgaacac
     7141 gatgcgtgtg gggttggttt agtcgttaat atcaacggcg aaaaatccca ctctattgtt
     7201 gtaaaaaagg gctacaagta ctcgaaaaca tggtacacag aggagctgaa ggggctgaga
     7261 aaaataccgg tgacggtgcc ggaatactag tacaaatacc tcacgagttt attcttttac
     7321 aaggtattgc cgttccagaa agaggaagat atggtaccgg acttgtgttt ttacctaaaa
     7381 acaatacagt acaaaaagat atactcaacc tattagaaac cattgcttta gaaaaaggat
     7441 tatctatact tgccata
//
LOCUS       DAOSRP010000096         7333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9461_length_7333_cov_4.323303, whole genome shotgun sequence.
ACCESSION   DAOSRP010000096 DAOSRP010000000
VERSION     DAOSRP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9461_length_7333_cov_4.323303"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLJ82_06685"
     CDS             <1..264
                     /locus_tag="PLJ82_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HOR41224.1"
                     /translation="SKVTTDLSKVDLEKTLISLNETLANLQLASSKFNEKSNTVGLLL
                     NDPSLYLNLNATMDSSNKLLIDLKENPKRYIHFSVFGAKKEKK"
     gene            556..1926
                     /gene="dnaA"
                     /locus_tag="PLJ82_06690"
     CDS             556..1926
                     /gene="dnaA"
                     /locus_tag="PLJ82_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOR41225.1"
                     /translation="MNSLYTTFWDKCLNIIRDNVNETSFSTWFEPIIPIKYENNVFTI
                     QVPSQFFYEYIEAQYADLLKSTLNRVIAPNTKLVYRVVVDNNNKKNGTTDVPGTEKAP
                     FQGKKKIGVPEFDSYLNFNYHFDNFVEGDCNRLPRTAGINIASQPGKTIFNPLFIYGK
                     SGVGKTHLANAIGLKTKELHPQKKVLYVSANLFQLQYTEASRNNQINDFLNFYQLIDV
                     LIIDDIHELGEGKKVSTQNTFFHIFNHLHQSGKQLIITCDKKPAELEGLEDRLLTRFK
                     WGLSAEIEKPDFETRKAILLSKTERDGLKIPDNVLSFIAEHITDSIRDLEGAIISLIA
                     QSTMNQKVINLDLAKKVVRNLVNVTEKKHTVKNIRDLVCEYFKIPVESLQSSSRKREV
                     VQARHIAMYFSKQLTQESLTTIGNSIGKRNHATVIHACKTVKDLMDTDKTFRGNVLEI
                     EKKLTF"
     gene            2042..2605
                     /locus_tag="PLJ82_06695"
     CDS             2042..2605
                     /locus_tag="PLJ82_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41226.1"
                     /translation="MLYKFIFISDEVDNFMREITIDSAATFLDLHKAILESVSYTEEG
                     MASFFICNDYWEKEQEITLIEMDTSSEYDNYVMEQTGLEEFISEEKQKILYVFDYFTD
                     RAFFIELKEIIPTKRQEKALCTKKMGEAPSQFVADIFEEDKVTKPAKVVTPLDENFYG
                     DEDFDMDELDEESFSDVDFSEESDPLK"
     gene            2602..3501
                     /gene="miaA"
                     /locus_tag="PLJ82_06700"
     CDS             2602..3501
                     /gene="miaA"
                     /locus_tag="PLJ82_06700"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR41227.1"
                     /translation="MKHLVVLLGPTGVGKTHASIQLAQTYNAPILSADSRQLFKGIEI
                     GTAAATKEEKDLVPHYFVGTKELDETYSAGAYEEDVLALLPTLFQTHDIVLLVGGSMM
                     YIDAICKGMDDIPEVDQSIRQNVHKLYQENGLESVRELLLKLDPAYYSTVDLKNTQRV
                     MHAVEVSLMIGKPYSSLLTKKTANRNFTITKIGFNLPREELYKRINMRVEQMFEKGLV
                     EEAQNLYTKKELNSLNTVGYKEIFDYIDGIYPSIEIAKEKIKQHSRNYAKRQLTWFRK
                     DKSIVWFHPDDVDGCIKHINACL"
     gene            3492..4175
                     /locus_tag="PLJ82_06705"
     CDS             3492..4175
                     /locus_tag="PLJ82_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41228.1"
                     /translation="MPLKALIISLLFLCSYATAQTSLLHRIGIGHNPVKRTYAEISTV
                     ASYTSTCWELEAGLNWQGNTYYGSSIQAFTLGALYNLPVSQPISVGIHYMYHPHNQLL
                     LSEQNLALMATYMPQHLDVYIGSYIRHFTSKKFSNQSVSELGYLLYQLNVYVFPKNYI
                     YNAMIGVRSYDTFEIEQAGNPMYVLQLSYRPSTIHYFLEGCVKPAGFYNIHVNPFHTQ
                     LRGGILWTL"
     gene            4163..5008
                     /locus_tag="PLJ82_06710"
     CDS             4163..5008
                     /locus_tag="PLJ82_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR41229.1"
                     /translation="MDFIKKRTLQSCIVILLIVTQSCNKLPEFDPLNFVRTNYEVDKR
                     FDDSQTLNDQSPLPDIQVMSETYRVVGASDLHITGNSEQVERFFSYTEEEDPSLVLIV
                     GDISNGLSHQMTSANNQFKAWNQLPSYFVAGNHDLFFSWSQYVQLFGSSTYTFTVHTP
                     TATDLYIGLESGSGTLGKKQTAWLVEQLQKRYEYRHCIVFTHTNFFARQFDTNGIYTK
                     EERMALLSLFAQYEVSMVISGHSHKENRTTFGNVHYITMPPLVDGGYMVFEVEESITT
                     RTMQL"
     gene            complement(5071..6276)
                     /locus_tag="PLJ82_06715"
     CDS             complement(5071..6276)
                     /locus_tag="PLJ82_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOR41230.1"
                     /translation="MNDVVKDIQISQYDYDLPDIRIAKYPLSQRDSSKLLVYKQGKID
                     QTVFSSISTHLPPRSLLVYNNTKVIHARLFFQKDTGAIIEIFCLEPIQPSDYQMVFQS
                     TKRCTWKCMVGNLKKWKQGALTLVCDVKGVSIELVAERDMINSQIHIHFSWNNVGVTF
                     GDILEAIGKLPIPPYLHRESEENDEITYQTVYSKEQGSVAAPTAGLHFTDEVLARLHQ
                     TGICTTEITLHVGAGTFLPVKSATIGEHTMHTEMVVISKQTIKHLQASIGNIIAVGTT
                     SVRSLESLYHIGVQVSKQQYHLSVSQWEPYSDHQLLSPFEALQAVIQYLNNNGLTEIR
                     FSTNIIILPSYQRKIVTGLITNFHQPKSTLLLLIAAFVGEDWKKMYEYALNNDFRFLS
                     YGDSSLLLP"
     gene            6404..6898
                     /locus_tag="PLJ82_06720"
     CDS             6404..6898
                     /locus_tag="PLJ82_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOR41231.1"
                     /translation="MTRIGLLSDTHSYWDDAYLRHFKDCDEIWHAGDIGSISIIEQLA
                     STGKKIRAVYGNIDGQDVRGQFPLHNRFTVEEVTVWMTHIGGYPAKYNPNIIAELTKK
                     PPMLFVCGHSHIAKVMYDKSLQMLHINPGAAGKQGFHAVRTLMRFVVDKEVMKDLEVI
                     ELSQ"
     gene            complement(6887..>7333)
                     /locus_tag="PLJ82_06725"
     CDS             complement(6887..>7333)
                     /locus_tag="PLJ82_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOR41232.1"
                     /translation="READFSTCELQYIDKDFRYSFSQQIRYYVRLNVYNADATCKADT
                     SYVIDIVDSFLEIPNVFTPNGDGRNDEFRVAYKSLIKFSGKVYDSWGRLVFSWTDPTK
                     GWDGTINGLKAPEGAYFYHIEATGVDRDEKGNLIEYKKTGDINLLR"
BASE COUNT         2628 a         1271 c         1237 g         2197 t
ORIGIN      
        1 tcaaaagtaa caaccgattt gagcaaggtt gacttagaga aaacacttat ttcgcttaat
       61 gaaacattag ctaatttgca attagcctcg tcaaaattta atgaaaagag caatactgta
      121 gggcttttgc tgaacgaccc atcgttgtat ctcaatttga atgcaaccat ggatagttct
      181 aataaattgc taattgattt aaaagaaaat ccgaaacgct atattcattt ttctgttttt
      241 ggggcaaaaa aagaaaaaaa ataagctttc aacaatctta acattaagtt aacattcaga
      301 tgaaacttct tactataaag gagtttcatc tttttgactc ataaattaac atatttttta
      361 aaagcctaac tgtcaataaa ttgatagttt ttgaaactat atatatatct gattatcaac
      421 aacttaaaaa caagtaactg aaagacaaat agatacaaat atttaaaaaa aacaagttta
      481 aaatgatttt tttttttgaa taatttttat gaaatttgca acccaacgat aaatttttta
      541 cataaaaaaa cgactatgaa ttctctttac actacttttt gggacaaatg cttaaacatt
      601 attcgagata atgtgaacga aacaagcttt agtacatggt ttgagccaat catacccatc
      661 aaatacgaaa ataacgtatt taccatccaa gtcccaagcc agttttttta cgaatacata
      721 gaggcacagt atgctgattt gttaaaatca acgctaaacc gtgtcattgc accaaataca
      781 aaactggtat acagagtagt cgtagacaac aacaataaaa aaaatggaac tacagatgta
      841 cctggcaccg aaaaggctcc ttttcaagga aaaaagaaaa ttggggttcc agaatttgat
      901 tcatatctaa actttaatta ccattttgat aattttgtag aaggtgattg caaccgtttg
      961 ccacgtacag caggtattaa tattgcaagt caaccaggta aaactatttt taatccgcta
     1021 tttatctatg gcaaatcagg tgtaggcaaa acacacttag ccaatgctat tggattaaaa
     1081 acaaaagaat tacaccctca aaagaaagta ttatacgtat cagctaattt atttcaatta
     1141 caatataccg aagcatccag aaacaatcaa atcaacgatt tccttaattt ctatcaacta
     1201 attgatgtgt tgataattga cgatatccac gaattaggcg aaggtaaaaa ggtaagtaca
     1261 caaaacacat tctttcatat attcaaccac ttacaccaat caggcaaaca acttattatt
     1321 acctgcgaca aaaagcctgc cgaattggaa ggattagaag accgtttgct aacacgtttc
     1381 aaatggggat tatcagctga aatagaaaaa cctgattttg aaactagaaa ggcaattttg
     1441 cttagcaaaa cagaaaggga cggattaaaa ataccagata atgtactttc atttattgcc
     1501 gaacatatta cagacagtat tcgtgattta gaaggagcta ttatttcgct tattgctcaa
     1561 tcaaccatga atcaaaaagt aataaactta gatttggcaa aaaaagtagt gcgtaatcta
     1621 gtcaatgtta cagaaaaaaa acacactgta aaaaatattc gtgatttggt atgtgagtat
     1681 tttaaaatac ctgttgagag tctgcaatca agctcacgca aacgtgaagt ggtacaagca
     1741 cgacacattg ctatgtattt ttctaaacaa cttacgcaag aatctctcac cacaattggt
     1801 aattcaattg gcaaacgcaa tcatgctaca gttattcatg cgtgcaaaac agtaaaagac
     1861 ctcatggaca cagacaaaac ctttagagga aatgtgttag aaatagaaaa aaaattgact
     1921 ttttaagcat ctcagcaaaa agcgaccaaa acggtcgctt tttttattga caatgtttct
     1981 caaaaagaaa aaaagctgta tttttacgcc tataattata tatatgcaaa attaaactag
     2041 tatgctatac aaatttattt ttatttcaga cgaagtggat aattttatgc gtgaaattac
     2101 aattgattcg gcagccactt tcttagattt acacaaagct attttggaat cagtatcgta
     2161 cacagaagaa gggatggctt ctttctttat ttgtaatgat tactgggaaa aagaacagga
     2221 aattacgctt attgaaatgg ataccagctc tgaatatgat aattatgtga tggaacaaac
     2281 aggtttggaa gaatttattt cagaagaaaa acaaaaaatt ttgtatgtgt ttgattattt
     2341 tacagacaga gcttttttta ttgaactaaa agaaataatt cctaccaaaa gacaagaaaa
     2401 ggcactatgt acaaaaaaaa tgggagaagc tccatcgcag tttgtagctg acatatttga
     2461 agaagacaaa gtaacaaaac cagcaaaagt cgttactccc cttgacgaaa atttctatgg
     2521 tgatgaagat tttgatatgg atgaacttga tgaagaaagt ttcagcgatg ttgatttttc
     2581 agaagaaagt gaccctctaa aatgaaacac ttagttgtac ttttgggtcc tacaggtgtt
     2641 ggaaagacac atgccagtat ccaattggca caaacataca atgcccctat tctgtcagca
     2701 gattcgcgtc aattatttaa aggcatagaa attgggacag cggctgccac caaagaagaa
     2761 aaagacttgg taccgcatta ttttgtagga accaaagaat tagacgaaac atatagtgct
     2821 ggagcatacg aggaagatgt actcgcttta ttaccaacat tatttcaaac gcatgatatt
     2881 gtgttacttg tgggtggatc tatgatgtat attgatgcaa tatgtaaggg catggatgat
     2941 atacccgaag tggatcaatc aatccgtcaa aatgtacaca aattatacca agaaaatggg
     3001 ttggaaagtg tacgggaatt attattaaaa ctggatccag catattattc aacagtagat
     3061 ttgaaaaata cacaacgtgt aatgcacgct gtagaagtta gcttaatgat aggcaaaccc
     3121 tactcgtctc tactaacaaa aaagactgca aacagaaact tcactattac taaaattgga
     3181 tttaacttgc ccagagaaga gttgtataaa cgaatcaata tgcgagtaga acaaatgttt
     3241 gaaaaaggat tggtagaaga agcacaaaac ctatatacca aaaaagagtt aaactctttg
     3301 aatacagttg ggtacaaaga aatatttgat tatattgatg gtatataccc aagtattgag
     3361 atagcaaaag aaaaaataaa acaacactca agaaactatg ctaaaagaca attaacctgg
     3421 tttcggaaag ataaaagcat tgtatggttt catccagatg atgtagatgg ttgtatcaaa
     3481 catatcaatg catgcctttg aaagctctta taatcagttt attattcctt tgttcatatg
     3541 ctacagctca aacaagctta ctgcatcgta taggtattgg acataaccct gtaaaaagaa
     3601 catatgcaga aattagcaca gtagcaagct atacaagtac ctgttgggaa ttggaagcgg
     3661 gactcaattg gcaaggaaat acatattatg gttcttccat acaagccttt acattgggag
     3721 cattgtataa tttacctgtc tcacaaccta tttcagtagg tatacattac atgtatcatc
     3781 cccataatca acttctatta agtgagcaaa accttgcttt gatggcaaca tatatgcctc
     3841 agcatcttga tgtgtacatt ggctcttata ttcgacattt cacatcaaaa aagtttagta
     3901 atcagtcagt tagtgaatta ggatatttac tttaccaatt gaatgtatac gtatttccaa
     3961 aaaattatat atacaatgct atgattgggg tacgtagtta tgatactttt gagatagagc
     4021 aagctggcaa tccaatgtat gtactacagc ttagttatcg ccccagtact attcattact
     4081 tcttagaagg ttgcgttaaa ccagcaggtt tttataatat tcatgtaaat ccatttcaca
     4141 cacaactaag aggaggtata ctatggactt tataaaaaaa cgaaccctac aaagttgcat
     4201 cgttatcttg cttatagtaa ctcaatcgtg taataagcta cccgaatttg accctttaaa
     4261 ttttgtaagg acaaattatg aagttgataa acgatttgac gactcacaaa ctctcaacga
     4321 ccaatcgcca cttccagaca ttcaggttat gagcgaaaca tatcgtgtag tcggagcatc
     4381 cgatttacat attacaggca attcagagca agtagaaagg ttcttttcct acaccgaaga
     4441 agaagatcct tcgctagtat taatcgttgg agatatttcc aatggattat cgcatcaaat
     4501 gacatctgct aacaatcaat ttaaggcgtg gaatcaactc ccctcttatt ttgtggcagg
     4561 caaccatgat ttatttttta gttggagcca atatgtacaa ctttttggaa gcagtacgta
     4621 cacctttacg gttcataccc ctacagccac tgatttgtat atcggcttgg aaagtggcag
     4681 tggtacttta ggaaaaaaac aaacagcctg gcttgtcgaa caactacaaa aacgttatga
     4741 atatcgtcat tgtattgtgt ttacacacac caactttttt gcacgacagt ttgacacaaa
     4801 cggcatatac accaaagaag aacgaatggc tttgctctca ttatttgctc aatacgaagt
     4861 atcaatggtt ataagcggtc attcacataa agaaaaccgt acaacatttg gcaatgtgca
     4921 ctatattact atgcctccat tggtagatgg tggatatatg gtgtttgaag ttgaagagtc
     4981 aataacaact cgcaccatgc aactttagac catattgata cgtgtattcg ttttatacat
     5041 tcaaaaaaaa tgaagcataa tcaggattaa ttatggcaaa agtaaagaac tatcaccata
     5101 actaagaaaa cgaaaatcat tattcagtgc atattcatac atttttttcc aatcctcacc
     5161 cacaaaagcg gcaataagca acaatagagt acttttgggc tgatgaaaat tagtgattaa
     5221 gccagtaact atttttcgtt ggtaagaagg taaaataata atattcgttg aaaaacgaat
     5281 ttctgttaat ccattattat tcagatactg tattactgct tgtaaagcct caaaaggaga
     5341 tagtaactga tgatcagaat aaggttccca ttgagaaaca gatagatgat actgttgttt
     5401 tgatacttgc acacctatat gatacaatga ctccaacgaa cggacactag ttgtaccaac
     5461 agcaataata ttaccaatag atgcttggag gtgtttgata gtttgtttgc taataaccac
     5521 catttcagta tgcatggtat gttctccaat agtagccgac ttgactggta aaaaagtgcc
     5581 agcacctaca tgcaacgtaa tttctgtagt acagatacct gtttgatgca agcgtgctag
     5641 tacctcatct gtaaaatgca aaccagccgt aggtgcagcc acagaacctt gctctttgga
     5701 atataccgtt tgatacgtta tttcatcatt ttcttccgat tcacgatgca aataaggagg
     5761 tataggcaat tttcctatgg cttcgagtat atcaccaaat gttacaccaa cattattcca
     5821 cgaaaaatgt atatgtattt gcgaattaat catatcacgt tcggcaacta gctctatgga
     5881 cacacctttc acatcacata ccaaggttaa agctccttgt ttccactttt ttaggtttcc
     5941 taccatacat ttccatgtac aacgttttgt tgattgaaaa accatttgat aatcagaagg
     6001 ttgtataggc tccaaacaaa aaatctcaat gatagctccc gtatctttct gaaaaaataa
     6061 acgagcatga attacctttg tattattata taccaacaaa ctacgtggag gtaaatgtgt
     6121 agagatagaa gaaaatactg tttgatcaat ttttccctgt ttgtatacta ataatttaga
     6181 agaatctcgt tggcttaacg gatatttagc tatacgtata tcaggtaaat cgtaatcata
     6241 ttgggaaatt tggatatctt ttacaacgtc attcatggat aaaattttgg tacaaaaata
     6301 caaaaatagc tagatatata ggaataataa cgcattttga aaaattaaaa acaagtcatt
     6361 tataaaattt acctatcttt acgtacttaa aattttgttc aacatgacac gtattggttt
     6421 actatcggac acgcatagtt attgggacga tgcataccta agacacttca aagattgtga
     6481 tgaaatatgg catgcgggag atattggtag tataagcatt attgaacaac tagcatcaac
     6541 gggaaagaaa ataagagccg tttatggaaa tatcgacgga caggatgtac gtggacaatt
     6601 tccattgcac aaccgtttta ctgtcgaaga agtaactgta tggatgacgc atattggcgg
     6661 atatcctgca aaatacaacc cgaacattat agctgaactt accaaaaaac ctcctatgct
     6721 atttgtatgt ggacattcgc acatagcgaa agtaatgtat gataaatcct tgcagatgct
     6781 acacattaat ccaggtgctg caggtaaaca agggtttcat gcagtacgta cccttatgcg
     6841 gtttgttgta gataaagaag tgatgaaaga tttagaagta attgaattat cgcaataaat
     6901 tgatatcccc ggtcttttta tattcaatca gattgccttt ttcatcccta tctactccag
     6961 ttgcttcgat atgatagaaa taagcccctt caggagcttt caatccattt atagtaccgt
     7021 cccagccttt agtaggatct gtccaagaaa aaactaaacg tccccaacta tcgtatactt
     7081 tgccactaaa ctttatcaat gatttgtaag ctactctaaa ttcatcattt cgtccatctc
     7141 cattaggcgt aaacacattg ggtatttcta aaaaagaatc cacaatatct atcacataac
     7201 tcgtatcagc tttacatgta gcatctgcat tgtatacatt taaccgtaca taataccgta
     7261 tttgttggga aaacgaatac cgaaaatctt tatcaatata ctgtaattcg caggtagaaa
     7321 aatctgcctc acg
//
LOCUS       DAOSRP010000097         7233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9619_length_7233_cov_4.663416, whole genome shotgun sequence.
ACCESSION   DAOSRP010000097 DAOSRP010000000
VERSION     DAOSRP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9619_length_7233_cov_4.663416"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLJ82_06730"
     CDS             complement(<1..650)
                     /locus_tag="PLJ82_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOR41233.1"
                     /translation="MKKFFTREAKIGLLVIVALGLLYFGINYLKGINLFKPTKYFYVQ
                     YDRIDGVVKTSPVLINGYKIGHVSNIDFDYTKNAPITLEISVDKHLIVPKGSYAELYE
                     TGMLGDKAIQLVLTDNPDIHQIGDTLPGKTSGGLIGEIVNSLLPPIQKVIPQVDSTLL
                     ALRKVIESGAVENSMKNIETTTQELASMSQSLKKIVHTDVPVIMNDVSTVTQNLSKV"
     gene            complement(661..1809)
                     /locus_tag="PLJ82_06735"
     CDS             complement(661..1809)
                     /locus_tag="PLJ82_06735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOR41234.1"
                     /translation="MNTLKKVIASFTFILFCTSSFAFTLVLDAGHGGQDAGAIGSFSK
                     EKNINLTYVLALGEKIAANHKDVKIIYTRTTDVFIPLNERAQIANKANADLFMSIHTN
                     SAKNKTANGTETFTLGHARSKENMELAMLENSVILLEEDYQKTYQGFDPNSTDSYIMF
                     QFMQDQYMEQSIKFADLIQQNFSKNTPLTDRGVRQAGFLVLRATTMPSVLVELGFISN
                     REEEKYLNRKENQDKMVDAIYEAFVNYKRVLEKKNGGQPIEHGKNTTIHTTPTPQQTT
                     TSTPTQKPTNTSTVVTESNILFKVQFLVSKKKYKEGNAIFKGVTNIDSYVENGFYKYT
                     TGSTSDIDAINQLKNDVAKKFPDAFVIAYKNGKRCNIHDAIKEVSDKK"
     gene            2015..4678
                     /locus_tag="PLJ82_06740"
     CDS             2015..4678
                     /locus_tag="PLJ82_06740"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOR41235.1"
                     /translation="MKKFCLFFFFLLFVGKSDVALFAQSNENVAMPTERTSTRISSKS
                     VIEDNIVYNATDSIIILKNGIIHLHGNASVTYQDIELKAAYIRLNIDSSLVFASGITD
                     TVGTYLGAPIFKEGSETYESKSLLYNFNTKKGLIFNTVTQQGEGFVSSGVTKKSTENV
                     FCLQHGKYTTCDHHDHPHFYLSLTKAKMKQGEYIVTGPAYLVIEDLPLPLFVPFGYFP
                     VPKTYSSGVLFPTFGDELQRGFYARNGGYYFAINDYVDLALTGDVYTKGTWSLNAASS
                     YVLRYKFRGNVNVSLMENIVGERDMPDYAKSNDFRIMWSHAQDAKANPFSTLSASVNF
                     STSSYERNNLNSYYNPNLLSQNTRSSTITYTQRFPESPFSLSASVYANQRTRDSIISL
                     SLPNLNVSMSRIYPLKRKQVVGTDRWYEKISMTYTGRFSNSITTKEDKLLTSSFTKDW
                     KNGVEHTLPISASFTVLNYFTVTPSFSSKLRWYFSQVNQTWQGDALTGSIVTDTLNGF
                     YNVFDYSASVSMQTKLYGFYKPMERIFGSRINMIRHVITPSISVSYKPDYGLPAWGYW
                     GTYQRPLTDGTFSQVFYDKYQSQLFGTPSRGEAGTVGFSIANNLEMKVKNKQDSQKEF
                     TKISIIDNLSVSSSYNLIADSLNWSNPNVSLRLKLHKQYTLNLSLLFDPYTYQLNEYG
                     NPKKVNITQLEKNGVLGRLMSTGTSFGYTFSNATFKKKEPSTNKGGLTPNEEPVVSVL
                     EKEINELNPIESKKLSKSTMDAEGYQKFSMPWSLNVNYSMRYAYASFNTASMEYNRAL
                     SHDINVSGNIQFTENWRFTASSYYNVNTNQWSYVNCSISRDLHCWSMSASFVPIGLYK
                     SYNFMIGIKSTLLRDIKYEKQSNPYDNQVWY"
     gene            4715..5317
                     /locus_tag="PLJ82_06745"
     CDS             4715..5317
                     /locus_tag="PLJ82_06745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOR41236.1"
                     /translation="MNEKKSFVALTYDLYVGEENEKELMEQATEEQPLTFYTGLGMML
                     DKFEAEIAPLKVGDSFDFVIPVDDAYGEYEDEKIIELPKNIFEVDGAIDEEMIAEGNM
                     VPLVDSEGNRINASIVSVGEDIVTVDINHPLAGEDLHFIGKVIEMREATEEELKAVFS
                     SGCGCGSGGCDSGDCGSGGCGCSSGECDSDDMYGCNCGCN"
     gene            5354..6436
                     /gene="aroC"
                     /locus_tag="PLJ82_06750"
     CDS             5354..6436
                     /gene="aroC"
                     /locus_tag="PLJ82_06750"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOR41237.1"
                     /translation="MINSFGTIFKLTSFGESHGKALGGVIDGCPAGVSIDEDFIQQEM
                     DRRRPGQSRLTTARNEPDSVEFLSGIFEGKTTGTPIGFVVYNQNQRSEDYDHLKDVYR
                     PSHADYVYQEKYHNRDYRGGGRSSARETVSRIVAGAIAKLALKQIMQGIDIKAYTAQV
                     GDIALDKPYQAYDLSTIENNVVRCPDAKVAEKMIELIAETKKQGDTIGGVITCVVKGV
                     PLGLGEPVFGKLHALLGSAMLSINAVKGFEYGMGFAGVSSNGSKVNDTFYVDNGVVKT
                     KTNNSGGIQGGISNGQDIYFNVAFKPVATILQEQEVLTRNLEVQVQKVRGRHDSCVLP
                     RAVPIVEAMAAMVIFDAYLMAKVRTL"
     gene            6446..>7233
                     /locus_tag="PLJ82_06755"
     CDS             6446..>7233
                     /locus_tag="PLJ82_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOR41238.1"
                     /translation="MTNETTNKQWAIVVNPHAGGKKAFQFWQKLSVALREQGIGYDIA
                     FTEGPFHAVQLAKKFRHQAYEQFLVIGGDGTLSEVVHGVFSCSVPPNPTITLSIVPIG
                     TGNDWARFWGITPTIERVVQSLLNHKTQTIDIGQISFVTYQGIPQTRYFINAMGEGFD
                     AKVAKLTTYLGSIFKGKSWLYSLSVFIVPFVYRAKPIHIEFDNGLLVQKKIYTLSCGN
                     GCYTGGGIKQTPHAVPTDGLFDVMMVESPSLWGIITGIHALFKG"
BASE COUNT         2199 a         1190 c         1474 g         2370 t
ORIGIN      
        1 acttttgata aattttgtgt aacggtactc acatcgttca taattacggg aacatccgta
       61 tgtactatct ttttgagcga ttgcgacatg gatgcaagtt cttgtgtggt agtttcaata
      121 tttttcatgc tattttctac tgcaccactt tcaattactt tgcgaagagc caataatgta
      181 gaatcaactt gtgggatgac tttttggata ggaggtaata acgagttaac aatttcacca
      241 attaatcctc cagatgtttt accaggtagg gtgtcgccaa tttggtgtat atcaggattg
      301 tctgtcaaaa cgagttgtat ggctttatcg cccaacatac ctgtttcata aagctcagca
      361 tacgaacctt tgggcactat taggtgttta tcaacagata tttcaagggt aattggagca
      421 ttttttgtgt aatcaaaatc aatattgctt acatgtccaa ttttatatcc gttaatcaaa
      481 acgggactcg tttttactac gccatctatt cggtcgtatt gaacgtaaaa atattttgtt
      541 ggtttaaata gatttattcc ttttaggtaa ttaataccaa aatacagcag acctagtgct
      601 acaattacta ataatccaat tttagcttct cgtgtaaaga attttttcat agtatattta
      661 ttatttttta tcagatactt cttttatagc atcgtggatg ttacatcttt ttccattttt
      721 atatgcgatt acaaatgcat caggaaattt ttttgctaca tcgtttttaa gttggtttat
      781 ggcatctata tccgatgttg atcctgtggt atatttataa aaaccatttt ccacatagct
      841 atcaatattt gttactcctt taaaaattgc atttccctct ttgtattttt tcttcgacac
      901 taaaaactga actttaaata gaatattact ttcggtaaca actgttgagg tgttggtcgg
      961 tttttgtgtg ggtgtgctgg tggtagtttg ttggggtgtt ggtgtggtat ggatggttgt
     1021 atttttaccg tgttctatcg gttgacctcc attttttttc tccaatactc ttttataatt
     1081 aacaaatgct tcgtatatag catctaccat tttatcttga ttttcctttc tgttaaggta
     1141 tttttcttct tctctattcg agataaaacc aagttctacc aatacggaag gcatcgtagt
     1201 agccctaagt accaagaaac ctgcctgacg tactcctcgg tctgttaacg gagtgttttt
     1261 tgagaaattt tgctgtatca agtctgcaaa tttgatgctt tgttccatat attggtcttg
     1321 cataaactga aacataatat acgaatcggt tgaatttggg tcaaatcctt gataggtttt
     1381 ttggtaatct tcctctaata gaataacgga gttttcaagc atagctaatt ccatgttttc
     1441 tttgctacgg gcatgaccaa gtgtaaatgt ttccgtacca ttcgctgttt tattttttgc
     1501 agaattggta tgaatagaca taaataaatc ggcattggct ttgttcgcta tttgtgcacg
     1561 ttcatttaaa gggataaaca catcagttgt gcgggtataa atgattttta catctttgtg
     1621 attagcagca attttttcac ccaaggctaa aacgtacgtt aagttaatgt ttttttcttt
     1681 tgagaaggag ccgatagcac cagcatcttg acctccatga cccgcatcca gtactagggt
     1741 gaaggcaaat gaagatgtgc aaaacagaat gaaggtaaaa ctagctatga cttttttcaa
     1801 tgtattcata ttcattgggc tataattaaa atcagtaaga aatcatcaat tgtttgcaaa
     1861 aatagtggtt ttttaccgaa attttgggtt tttatcgtaa atatctccta aaaaaagatt
     1921 aaaatttatt attatatcct acaggtaact ttaaaatgta taaatttgca caagtttttt
     1981 taagttagca tgctcactat atagatattg tcagttgaag aagttttgcc tctttttttt
     2041 cttccttcta ttcgttggta aatccgatgt agcactattt gctcagagca atgaaaatgt
     2101 tgctatgcct accgaacgta cgagtactcg aataagctca aagtctgtta tagaggataa
     2161 tatcgtatac aatgccaccg attctattat cattttgaaa aatggaatta ttcatttaca
     2221 tggcaatgcc tctgttacgt atcaagatat tgaattaaaa gcagcttata tcagattaaa
     2281 catagatagt agtttggtgt ttgcatcggg tataacagac acagtaggga cttatcttgg
     2341 tgctcctatt tttaaagaag gatcagaaac gtatgagtca aaatcattat tgtataattt
     2401 taatactaaa aaaggattaa tttttaatac agttacacaa caaggagaag gatttgtaag
     2461 tagtggtgtt accaaaaaga gtactgaaaa tgttttttgt ttgcagcatg gtaaatacac
     2521 tacctgcgat caccacgacc atccccattt ttacttgtct cttacaaaag cgaagatgaa
     2581 acagggagaa tacattgtta caggacccgc ttatctggtg atagaagatc ttccgttgcc
     2641 tctttttgtg ccttttggct attttccagt tcctaaaacg tattcatcgg gagtgctttt
     2701 ccctactttt ggagacgaac ttcaacgtgg tttttatgca cgtaacggag gatattattt
     2761 tgccattaat gattatgttg atttagctct tacgggcgat gtatacacca agggtacctg
     2821 gtcgctaaat gcagcatcta gctatgtgct tcgttataaa tttagaggga atgtgaatgt
     2881 aagtttgatg gaaaatattg tgggtgaacg tgatatgcca gattatgcta aaagtaacga
     2941 tttccgcatt atgtggtcgc atgcacaaga tgctaaagca aatccatttt ccaccttatc
     3001 agctagtgtt aatttttcta cgtcttctta cgaaagaaac aatttgaata gttattataa
     3061 ccctaattta ttatcacaaa atacacgtag ttctaccatt acctatacgc aacgttttcc
     3121 agaaagtcca ttttctttat cagcaagtgt atatgctaac caacgtacac gcgattcaat
     3181 tatcagtttg tcgttgccta acttaaatgt gagtatgagt cgtatttatc cattgaaacg
     3241 aaaacaagtg gtgggtacag ataggtggta cgaaaaaatt agtatgacct atacaggtcg
     3301 attttctaat agtataacta caaaagaaga caaattactc acttcctcct ttaccaaaga
     3361 ttggaaaaat ggggtagaac atacattacc catttctgcc tccttcacag tacttaatta
     3421 ttttactgtt acgccatcgt ttagttcaaa gttacgctgg tattttagtc aggtaaatca
     3481 aacttggcag ggagatgcgt tgactggatc tattgtaacg gataccttga atggttttta
     3541 taatgtattt gattatagtg cctctgtcag tatgcaaacc aaattgtatg gtttttataa
     3601 accaatggaa cgcatttttg gtagtcggat taacatgata cgccatgtaa taaccccgtc
     3661 aataagtgtg tcatacaaac cagattatgg cttgcctgca tgggggtatt ggggaacata
     3721 ccaacgtccg ttaactgatg gcactttttc gcaggtattt tacgataaat accaaagtca
     3781 attatttggc actcctagtc gtggagaggc tggtacagtg ggttttagca ttgcgaacaa
     3841 tttggaaatg aaggttaaaa ataaacaaga ttcgcaaaaa gaatttacta aaattagcat
     3901 tatcgacaat ttgagtgtat cttcgagcta taatttgatt gctgattcgc tcaattggtc
     3961 gaatccgaac gtgagtttaa ggctcaaatt gcacaaacaa tataccttga acttgagttt
     4021 gttattcgat ccgtacacgt atcagttaaa tgaatatggt aatccgaaga aggtaaatat
     4081 tacccaattg gaaaaaaatg gggtcttagg acgtttaatg agtacgggta catcgtttgg
     4141 gtacacgttt agtaatgcta cttttaagaa aaaagagcca tcaacaaata agggaggatt
     4201 aacgccaaac gaagaacctg tagtgtcagt tttagaaaaa gagataaatg agttaaatcc
     4261 tattgaatcc aaaaagctgt caaaatcgac tatggatgca gagggatatc aaaagttttc
     4321 gatgccatgg tcgctcaatg tcaattattc gatgcggtat gcgtatgcta gttttaatac
     4381 tgctagtatg gaatataata gggctttatc tcatgatatt aatgtaagtg ggaatattca
     4441 gtttactgaa aattggcgtt ttacagcatc gtcttattac aatgtgaaca ccaatcaatg
     4501 gagttacgtt aattgtagta tttcgcgcga tttgcattgt tggtcgatgt cggctagttt
     4561 tgtgcctata ggattgtaca aatcgtataa ttttatgata ggtattaaat ctaccttact
     4621 acgtgatatt aaatatgaaa aacaaagtaa tccgtacgat aatcaagtat ggtattaaat
     4681 aaaccaagta ttaatttaca aaaaacaata aacaatgaac gagaaaaaaa gttttgtagc
     4741 tttaacatac gatttatatg ttggagaaga aaatgaaaaa gagttaatgg aacaagccac
     4801 agaagaacaa cccttaactt tctatacagg attgggtatg atgttagata aatttgaggc
     4861 agaaattgct cctttaaaag taggagatag ctttgatttt gttattccag ttgatgatgc
     4921 ttatggcgaa tacgaagatg aaaaaatcat tgaattgcct aaaaatatat ttgaagtaga
     4981 tggtgctatt gacgaagaaa tgatagctga aggcaatatg gtaccattgg tagattcaga
     5041 gggtaatcgt atcaatgcat ctatcgtatc tgtaggagaa gatatcgtta ccgtagatat
     5101 caaccatcct ttagctggcg aagatttgca ttttattggc aaagtgattg aaatgcgtga
     5161 agctactgaa gaagaattga aagcggtatt ttcttcggga tgtggttgcg gtagcggagg
     5221 ttgtgatagt ggcgattgtg gtagtggtgg atgtggttgc tcatcaggag aatgcgatag
     5281 cgacgatatg tatggctgta attgcggttg taactaaccc ttttttataa tttttaccaa
     5341 aagaaaaata agaatgataa attcatttgg aaccattttt aaattaacat cgtttggcga
     5401 gtcgcacgga aaagcattgg gtggggtaat tgatggttgc cctgctggtg ttagtatcga
     5461 cgaggatttt atccaacaag agatggatag acgacgtcct ggacagtcgc gcttaactac
     5521 tgcacgcaac gaacccgata gtgtagaatt tttatcaggt atttttgaag gaaaaacaac
     5581 tggtacgcct ataggatttg tggtgtacaa ccaaaatcaa cgctcagaag attatgatca
     5641 tcttaaagat gtgtatcgtc cttcgcatgc cgattatgtg taccaagaaa aataccacaa
     5701 tagagactac cgtggaggtg gacgttcgtc ggctagggaa actgttagcc gaattgtagc
     5761 aggtgccatt gcaaaattag cactcaagca aattatgcaa ggtattgata tcaaagcata
     5821 caccgcacaa gttggtgata ttgccttaga caaaccttac caagcatatg atttatctac
     5881 tatcgaaaac aatgtagtac gttgtccgga tgccaaagtg gctgaaaaaa tgattgaatt
     5941 gatagccgaa accaaaaaac aaggcgatac cattggtggc gttattacgt gtgtagtaaa
     6001 aggtgtacca ttggggttgg gcgagcctgt atttggtaag ctgcatgctt tgctgggcag
     6061 tgccatgctg tctatcaatg ctgtaaaagg ctttgaatat ggtatgggat ttgctggcgt
     6121 atcgagcaat gggtcgaagg taaacgatac cttttatgta gataatgggg tagtgaaaac
     6181 caaaaccaac aactctggtg gcatacaagg tggtatttcc aacggacaag atatttattt
     6241 taatgtagcc tttaaacccg tagctactat tttgcaagaa caagaagtat tgacccgcaa
     6301 cttagaagta caagtacaaa aagtacgcgg cagacacgat tcgtgcgttt taccacgtgc
     6361 agtacccatt gtagaagcca tggcagccat ggtaattttt gatgcgtatt taatggctaa
     6421 agtgcgtacg ctatagtggt tttggatgac aaacgaaaca acaaataagc aatgggcaat
     6481 tgttgtcaat ccgcatgctg ggggcaaaaa agcttttcag ttttggcaaa aattgtctgt
     6541 tgctttacgt gagcagggca tagggtatga cattgcgttt accgaaggac catttcatgc
     6601 cgtacagttg gctaaaaagt tccgtcatca ggcgtacgaa caattcttag tcattggtgg
     6661 tgatggtacc ttgagcgagg ttgttcatgg tgtttttagt tgttctgttc ccccaaatcc
     6721 caccattact ttatctattg tacccattgg tacgggcaac gattgggcac gtttttgggg
     6781 cattacgcct actatcgaaa gggtggtaca gtccttactc aaccataaaa cccaaaccat
     6841 tgatatcggt caaatttcat ttgtaaccta tcagggcata ccacaaacac gttattttat
     6901 caatgccatg ggcgagggtt ttgatgccaa ggtagccaag ttaaccacct atttaggttc
     6961 catttttaaa ggaaaatcgt ggttgtatag cctgtcggta tttattgttc cttttgtgta
     7021 tagagccaaa cctatacata ttgagtttga taacggattg ctggtacaga aaaaaatcta
     7081 caccttatcc tgcggaaacg gttgctatac aggtggaggc attaagcaaa caccacatgc
     7141 tgtacctacc gatggattgt ttgatgtgat gatggtagaa agtccttcgt tgtggggtat
     7201 tattacaggt attcatgcct tattcaaagg caa
//
LOCUS       DAOSRP010000098         7177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9718_length_7177_cov_4.705701, whole genome shotgun sequence.
ACCESSION   DAOSRP010000098 DAOSRP010000000
VERSION     DAOSRP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9718_length_7177_cov_4.705701"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1579
                     /gene="miaB"
                     /locus_tag="PLJ82_06760"
     CDS             251..1579
                     /gene="miaB"
                     /locus_tag="PLJ82_06760"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOR41239.1"
                     /translation="MKNQKKLFIETYGCQMNVADSEVVASIMGMSGYELTDNINEANA
                     IFINTCSIRDNAEQKAFSRLDYFQSLKKKRKDIIIGVLGCMAERIKEDLLERKVIDMV
                     VGPDAYLDLPNLIGAVESGEKAINIELSTTETYQDIIPQRIGHNRISGFVSIMRGCNN
                     FCSYCIVPYTRGRERSRPATSIINEMKDLVAKGYKEITFLGQNVNSYLDSSEDNKYTF
                     PDLLAMAANTFPDTRIRFTTSHPKDISEELIRTIATYHNLCKHIHLPVQSGSNRILSL
                     MNRKYTREEYLQKIAMIKRIIPECGITTDVFTGFHSETEADHQETLSLMQEVGFDNAF
                     MFKYSERSGTYAAKHLEDNVPEAIKLSRLQEVVDLQNKLSKESNQKDLGKIVEVLIEG
                     YSKKSRNQLFGKSSENKVVVFNKENKKIGEYTKIRIKDATVATLLGEICE"
     gene            1685..3052
                     /locus_tag="PLJ82_06765"
     CDS             1685..3052
                     /locus_tag="PLJ82_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR41240.1"
                     /translation="MKKLFTFLIVSVLTITSLSAADYTHWSVYIDGGASQFDGDVSQR
                     YNQIHPGADIHWTAGLGVEYTFTPFWGLGVEYQYMPYSGYSTSTSQGWWSNYGEFDFE
                     GGSHQAHIFTSLNLTNLLNVYRKTWRFNVYANIGLGASFYNVSQSNIVPASIPPGGWT
                     FARAPKEVEDGRSLLVPMSLNFEYNISKPLAVGLNVHYRATNKDNYEGEAYVRGVQND
                     NFLLGTASLRYKFASGQKDGHIRNITADKFRQEETKEREILDKLDSLNNKVDDLDSVV
                     TNDLTPRMNEVERLLSDQPDADGDGVPDWRDREPNTPAGSFVNYWGESLPYEVLKATE
                     FYEELAKMQEEEVDYDMSVYFAFDKKNLTKRSIDNIAKAAQKLKSNPLLKVELRGYAD
                     YPGSHNYNLRLSTSRGEVVKQELVNKHGIDPSRITLNPQGRMTNPPNANQKNRRTDFV
                     FSKMK"
     gene            3132..3515
                     /locus_tag="PLJ82_06770"
     CDS             3132..3515
                     /locus_tag="PLJ82_06770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR41241.1"
                     /translation="MEEKEHIHYDQFAKNSGIKLVEAKNGEAIAKMEIRNMHLNGLNL
                     VHGGALFTLADFTLGAAVNSLGGAHVTMSASIHYVKSAKEGTLIGISKQVSAGRTIAT
                     YDVEIRLEETNDLIAIVRGNSFKKS"
     gene            3602..5068
                     /gene="cysS"
                     /locus_tag="PLJ82_06775"
     CDS             3602..5068
                     /gene="cysS"
                     /locus_tag="PLJ82_06775"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR41242.1"
                     /translation="MEHTLQIYNTLTRKKEVFIPIHSPNVGLYVCGPTVYGDAHLGHA
                     RPAITFDLLFRYLTHLGYKVRYVRNITDVGHLEHDADEGEDKVAKKARVEQKEPMEIV
                     QFYLNRYRKAMEQLNVLPPSIEPHASGHIMEQIEFTQKIIDAGFAYQSEGSVYFDVEK
                     YNKQYHYGKLSGRNIEELLETTRTLDGQSEKKKSVDFALWKKASPEHIMRWKSPWSDG
                     FPGWHMECSAMGTKYLGEEFDIHGGGMDLVFPHHECEIAQSTAALGKETVRYWMHNNM
                     ITINGQKMGKSLGNFITLDEFFGGSHPLLSQAFSAMTIRFFILQAHYRSTVDFSNEAL
                     QASEKGLNKLLETYARLQKITPSATSTIDVSGLRAKCEEAMKDDLNTPIVLSHLFEAS
                     KAINTVADGKASLSAKDVAELKSVFKLFVEDILGLQQTTKDNTQHEAYQKAIDLLLEL
                     RLEAKRNKDFALSDKIRNELTTLGFSIKDTKEGFEWSL"
     gene            5087..5557
                     /locus_tag="PLJ82_06780"
     CDS             5087..5557
                     /locus_tag="PLJ82_06780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOR41243.1"
                     /translation="MKKIQNPWTILDGYNCFACSPANEIGIKMEFFEEGEELICKWEP
                     SKHYEGFTNVLHGGIQATLLDEMGAWVVARKLQTVGFTSKLTIKYHKTVVINQPYIYI
                     KAKLTEVKRNLAFIEAHIYNYEEQLCASAEMCYFCTSQEAASSSFNFNGCSLEE"
     gene            complement(5559..6233)
                     /locus_tag="PLJ82_06785"
     CDS             complement(5559..6233)
                     /locus_tag="PLJ82_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOR41244.1"
                     /translation="MNLSVQISSSIASLLPHVVIGLLQCKVSNTSYNDELWTDIEDYS
                     KQFKQTYQLADINQRETIAATREAYRKTGKEPNRYRPSAEALSRRLVKGESLYRISTI
                     VDGINLISVKTGYSIGGFDRDSIIGNSLELGIGQAGEEFYAIGRGLLNIEGLPVYRDE
                     KGPIGTPTSDHERTKISLNTHHLVLIVNGFDGSITSVQQTLDFSRELLLKYTNASNIS
                     EKIVPA"
     gene            complement(6241..>7177)
                     /locus_tag="PLJ82_06790"
     CDS             complement(6241..>7177)
                     /locus_tag="PLJ82_06790"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HOR41245.1"
                     /translation="KDLGNLQQALNEAFEVKNNPYKWDTLGKNKTLLMVFFNSSLRTR
                     LSTQKAGMNLGMNTMVLDINQGAWKLETERGVIMDGDTTEHILEAIPVMGSYCDIIGV
                     RSFARFENKEFDCQDTILKQFIQYSGKPVFSMEGAIGHPLQAFADLITIEQYKKTDRP
                     KVVLTWAPHPKALPQAVPNSFADFMNEANVDFVITHPKGYELDEKFVRGARVEYDQRK
                     AFEGADFIYAKNWSAFQDPNYGKILSKDRDWTVDTEKMALTNNAYFMHCLPVRRNMIV
                     TDDVIESPQSIVIPQAANREVSAQTVIKRMLEALK"
BASE COUNT         2485 a         1255 c         1344 g         2093 t
ORIGIN      
        1 aggagagccg ctgcccccct tttaacctaa cctaaacctt aactatgaaa aaaaatcatc
       61 gtttcttttt cttgatccaa agaaaaacaa ttgttttgaa ttacgcaaac gtttgcatag
      121 tttctaacgc aaacgtttgc gtttttaacc ttatttaacg tttattcgtt gttttagcta
      181 aatattccaa gaatattcac tataaaaaat aaaaaacata cctttgtacc ctaattattt
      241 cggacataaa atgaaaaacc aaaaaaaatt atttatagaa acatacggat gtcaaatgaa
      301 cgttgcagac agcgaagttg tagcatcaat tatgggcatg agtggatacg agctcacaga
      361 caatataaac gaagcaaatg ccatcttcat taacacctgt tccatcagag acaatgcaga
      421 acaaaaagct ttttcacgcc ttgattactt tcaatcgtta aaaaagaaaa gaaaagatat
      481 cattattgga gtgttaggct gtatggctga aagaattaaa gaagatttac ttgaacgaaa
      541 agtcatagac atggttgttg gtccagatgc gtatcttgat ttgcctaatc tgatcggagc
      601 ggtagaatct ggagagaaag ctataaatat tgaactttcg acaaccgaaa catatcaaga
      661 tattatacca cagcgtattg gacataatcg aattagtggg tttgtgtcca tcatgcgagg
      721 atgtaataat ttttgttctt attgcattgt tccgtacaca cgaggcagag aaagaagtcg
      781 ccctgctaca agtattatta acgaaatgaa agatttagta gccaaaggtt acaaagaaat
      841 aacattctta ggtcaaaatg taaattcata ccttgattca tctgaagaca acaagtacac
      901 gtttccagat ttacttgcaa tggctgcaaa cacattccct gatacaagaa tccgcttcac
      961 cacttctcac ccaaaagata ttagcgaaga attaattcgc actattgcca cataccataa
     1021 tttatgcaaa cacattcatc tacctgtaca atcaggtagt aatcgtatat tgtccttaat
     1081 gaatagaaaa tatactcgtg aagaatattt gcaaaaaatt gctatgataa aacgcattat
     1141 cccagaatgt ggtataacca ctgatgtttt tacaggattc cattctgaaa cagaagccga
     1201 ccatcaagaa acgctttcac ttatgcaaga agttggattt gacaacgcct ttatgtttaa
     1261 atactccgaa cgaagtggca catacgcagc taaacacctc gaagataatg ttccagaggc
     1321 aatcaaatta tcgagactgc aagaggttgt tgatttacaa aacaaacttt ccaaagaaag
     1381 taaccaaaaa gatcttggaa aaattgttga agttttaatc gagggatatt caaaaaaatc
     1441 acgcaatcag ctttttggaa aatcctcgga aaataaagtc gtcgtattta ataaagaaaa
     1501 caaaaaaata ggcgaataca ccaaaatacg aattaaagat gccacggtag caacactttt
     1561 aggtgaaatt tgcgagtaat agtctaaaaa ttaggcaaaa atgaaaattt tattgaaaaa
     1621 aaagaaaaaa ataaaaatat tgtttttata tttgtcccct gaataattaa ctaaaacacg
     1681 ttatatgaaa aagttattta catttttgat agtttctgtg ctaacgataa cttcgttatc
     1741 agctgcagac tacactcatt ggtctgttta tatcgatgga ggagcttctc agtttgacgg
     1801 agacgtttca caaagataca atcaaataca ccctggagca gatatccatt ggacagctgg
     1861 attaggagta gaatatacat ttactccatt ttgggggtta ggcgtagaat accaatatat
     1921 gccttattct ggttactcaa cttcaacaag tcaaggatgg tggtctaatt acggagaatt
     1981 tgactttgaa ggaggatcgc atcaagcaca tatatttact tctttaaatc taactaattt
     2041 attaaatgtg tatagaaaaa catggaggtt taatgtctat gcaaacattg gtttaggtgc
     2101 ttctttctat aatgtgtctc aatccaatat agtaccagct agtatccctc caggtggttg
     2161 gacttttgca agagctccaa aagaagttga agatggacgt tcgttgttag ttcctatgtc
     2221 tttgaacttt gaatataata tttctaaacc attagcagtg ggtttaaatg ttcactatag
     2281 agcaacaaac aaagataatt atgaaggaga agcctacgtt agaggcgttc aaaacgataa
     2341 tttcttatta ggaactgcta gtttacgtta taaatttgct tctggacaaa aagatggtca
     2401 tatcagaaac ataactgctg ataaatttag acaagaagaa actaaggaga gagaaattct
     2461 tgacaagctt gattcattaa acaacaaggt agatgatctt gatagtgttg ttacaaatga
     2521 tttaactcca cgcatgaacg aggtagaaag attactttct gaccaaccag atgctgatgg
     2581 tgacggagtt ccagattgga gagatagaga accaaataca cctgcaggtt cgtttgtaaa
     2641 ctattgggga gaaagccttc cttatgaagt attgaaagct actgagtttt acgaagaatt
     2701 agctaaaatg caagaggaag aagttgatta cgacatgtct gtttactttg catttgataa
     2761 aaagaacttg acaaaacgtt ctattgacaa tattgcaaaa gcggctcaaa aattaaaatc
     2821 aaacccatta ttgaaagtag aacttagagg atatgctgac taccctggta gccacaacta
     2881 caacttacgt ttgagtactt ctcgtggtga agttgttaag caagagttag taaacaaaca
     2941 tggtattgat ccttctagaa tcaccttgaa tccacaagga agaatgacca atccaccaaa
     3001 tgctaaccaa aagaacagaa gaactgactt tgtgttcagc aaaatgaaat agaaagattg
     3061 tagtctttaa ttaatagaaa aagccctgag tcaacatgac gtcagggctt ttttaacaat
     3121 aacaacatat tatggaagaa aaagaacata ttcattacga ccaatttgca aaaaactcgg
     3181 gtattaaatt agtcgaagca aaaaatggag aagcaatcgc aaaaatggaa ataagaaaca
     3241 tgcatctaaa cggcttgaat ctggtgcatg gtggagcctt atttacctta gcagatttca
     3301 ctttaggggc agccgtcaac tctcttggag gagctcatgt aacgatgagt gcaagtattc
     3361 actatgtaaa atctgccaag gaaggaacac ttattggaat atctaaacag gtttctgcag
     3421 gtagaactat cgcaacatac gatgtagaaa tacgattaga agaaacaaat gacttaattg
     3481 caatcgttag aggaaattcg tttaagaaat cttagaaata tgaagaattc ttgtagaaaa
     3541 gcataattat agtagaataa aatatgtaat tttgcaggaa agaaaacaat cagttgaatt
     3601 tatggaacat acattgcaaa tttataacac actaactcga aagaaagaag tttttatacc
     3661 cattcatagc ccaaatgtgg gcttgtatgt atgtggacca actgtatatg gcgatgctca
     3721 tttaggtcat gcacgacccg ctattacttt tgatttactg tttcgttact taacccattt
     3781 aggctataag gtgcgttatg tacgcaacat aacagacgta ggacatcttg agcatgacgc
     3841 agatgaagga gaagataagg ttgcaaaaaa agctagagta gaacaaaaag agcccatgga
     3901 gattgttcaa ttctacctaa atagataccg caaagccatg gagcaattga atgtattgcc
     3961 accaagtatt gagccacatg cgtctggaca cattatggag caaattgaat ttacacaaaa
     4021 aattattgat gcaggttttg cctaccaaag tgagggttcg gtatattttg atgtagaaaa
     4081 atacaacaaa cagtaccact acggaaaatt gtcaggaaga aatatagaag aacttcttga
     4141 aacaacacga acacttgacg ggcaaagtga aaaaaagaaa agtgtggatt ttgccttgtg
     4201 gaaaaaagca tcgccagaac atattatgcg atggaaatcg ccatggagcg atggttttcc
     4261 gggctggcac atggagtgtt cggctatggg cacgaagtac ttaggagaag aatttgacat
     4321 acatggcgga ggtatggatt tagtttttcc tcatcatgag tgcgaaattg cacaatcgac
     4381 agccgcttta ggcaaagaaa ctgtacgtta ctggatgcac aacaatatga ttactattaa
     4441 tgggcagaaa atgggcaaat cgctaggtaa ttttattaca ttagatgagt tttttggagg
     4501 ttcgcatcca ctgctttcac aagcattttc agccatgacc attcgctttt ttattcttca
     4561 agcacattat cgtagcaccg tagattttag caacgaagca ttacaagctt ctgaaaaagg
     4621 acttaacaag ttgcttgaaa cgtatgcacg cttgcaaaaa ataactcctt cagccacatc
     4681 tactatcgat gtatctggtt tgcgagctaa atgcgaagag gctatgaaag atgatctaaa
     4741 tacaccgata gtattatcac atctttttga agcctcaaaa gccattaaca ctgtggcaga
     4801 tggaaaagcc agcttaagtg caaaggatgt agcagaacta aaatcagtat tcaagttatt
     4861 tgttgaagac atactgggat tacaacaaac gaccaaagac aatactcaac atgaagcata
     4921 ccaaaaagca attgacttgt tgcttgagct acgattagaa gctaaaagaa acaaagattt
     4981 tgccctatca gataaaatta gaaacgaatt aacaactctt ggttttagca tcaaagatac
     5041 aaaagaagga tttgaatggt cgctataaga ataaaaacct tgtaacatga aaaaaataca
     5101 aaatccatgg acaattctag atggctacaa ttgctttgct tgtagcccag caaatgagat
     5161 aggaataaaa atggaatttt ttgaagaagg agaagagttg atttgcaaat gggagccttc
     5221 aaaacactat gaaggattta ccaacgtatt gcatggaggt atacaagcca ccttgttaga
     5281 tgaaatggga gcttgggtgg tagctcgcaa attacaaacg gttggtttta cgagtaaatt
     5341 aactatcaaa taccataaaa cagtcgttat taaccagcct tatatttata tcaaagccaa
     5401 actaacagag gtaaaacgca atttagcatt tattgaagct catatttata attacgagga
     5461 gcaattgtgt gcctcagcag aaatgtgtta tttttgcacc tcacaagaag cagcatcttc
     5521 ttcttttaac tttaacggat gttcgttaga ggagtaagct atgccggtac tattttttct
     5581 gatatattgc tagcatttgt gtacttaaga agcaattctc tggaaaaatc gagtgtttgt
     5641 tgcacagaag taattgagcc atcaaaacca tttactatta gcacaagatg gtgtgtattg
     5701 agagaaattt ttgtacgttc gtgatcgcta gtaggagttc cgattggtcc tttttcatct
     5761 cgatatacgg gtaatccttc gatatttaac aaccctcttc ctattgcata aaattcttca
     5821 ccagcttgtc caatgccaag ttcaagagaa ttcccaataa tgctatctct atcgaatcca
     5881 ccgatggaat agcccgtttt aaccgagatt aaattaatac catccactat ggttgaaatg
     5941 cgatacaatg attctccttt cactaatcta cgagaaagag cttcggctga gggtctatat
     6001 ctattcggtt cttttcctgt ttttctatac gcttcacgtg tagcagcaat agtttctcgt
     6061 tggtttatgt cagctagttg gtatgtttgt ttgaattgct tagaatagtc ctcaatatct
     6121 gtccataatt catcattata agacgtatta gacaccttac attgcaacag accaataaca
     6181 acatgtggca ataaagatgc gatgctacta ctaatttgta ctgaaaggtt catactaagc
     6241 ttatttcaat gcctctaaca tacgcttaat aacagtttgt gcagacacct ccctattggc
     6301 ggcttgaggg attacaattg attgagggct ttcaattaca tcgtccgtaa caatcatatt
     6361 gcgtcttacg ggcagacagt gcataaaata agcattattg gtaagtgcca tcttttcggt
     6421 atctactgtc caatctctat ctttagaaag aattttacca taattagggt cttgaaaagc
     6481 cgaccagttt ttagcataga taaaatctgc cccttcaaac gctttgcgtt gatcgtattc
     6541 aacacgagca cctcttacaa acttttcatc aagctcatac cctttggggt gcgtaataac
     6601 aaaatcaaca ttggcttcgt tcataaaatc ggcaaacgaa ttgggtacag cttgaggcaa
     6661 agccttaggg tgaggagccc aagtgagcac aacttttggt ctatctgttt ttttgtattg
     6721 ttcaatggta attaaatcgg caaaagcctg caatggatgc ccgatagctc cctccatgct
     6781 aaatacgggt ttgccagaat attggataaa ttgttttaga atggtatctt gacaatcaaa
     6841 ctctttgttt tcaaaacgag caaacgaacg aacaccgata atatcgcagt aagaacccat
     6901 aacgggaata gcttctaaga tgtgctctgt agtatcgcca tccataatta caccgcgttc
     6961 tgtttctagt ttccaagctc cttgattaat gtcgagtacc atggtgttca tacctaaatt
     7021 catgcctgct ttttgtgtac ttaaacgggt acgtagacta gaattaaaaa ataccatcaa
     7081 caaagttttg ttttttccta gtgtgtccca tttataagga ttgtttttta cttcaaaagc
     7141 ctcatttaat gcttgttgca aatttcctaa atctttt
//
LOCUS       DAOSRP010000099         7118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9818_length_7118_cov_4.132805, whole genome shotgun sequence.
ACCESSION   DAOSRP010000099 DAOSRP010000000
VERSION     DAOSRP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9818_length_7118_cov_4.132805"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1281)
                     /gene="tgt"
                     /locus_tag="PLJ82_06795"
     CDS             complement(151..1281)
                     /gene="tgt"
                     /locus_tag="PLJ82_06795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR41246.1"
                     /translation="MTFELQHTDPKSNARAGLITTDHGQIETPIFMPVGTVASVKAVH
                     QHELKEDIKAQIILGNTYHLYLRPGIEILEKAGGLHKFNGWDKPILTDSGGFQVFSLS
                     HRRKMKEEGVTFHSHIDGSKHLFTPENVVDIQRIIGADIIMALDECTPGDADYAYAKK
                     SMELTHRWLARGIQHFDTTEPKYGYSQTFFPIVQGCVYPDLRKQSAEFVASQGREGNA
                     IGGLAVGEPTEKMYEMIEIVNEILPTDKPRYLMGVGTPANLLEAIERGVDMFDCVMPT
                     RNGRNGMLFTKNGIINMRNKKWADDFSPIEIDGASYVDTAYSKAYLRHLFISNELLGL
                     QIASIHNLAFYLWLVQEARKQILAGTFSVWKTNMIQQVSQRL"
     gene            complement(1286..2584)
                     /gene="mtaB"
                     /locus_tag="PLJ82_06800"
     CDS             complement(1286..2584)
                     /gene="mtaB"
                     /locus_tag="PLJ82_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOR41247.1"
                     /translation="MIDTSVFDNKTVAFYTLGCKLNFSETSAIGKSLIEQGYRKVAPH
                     EPASLCIINTCSVTDSADKKCRQTITKAIKENPGAFVIVTGCYAQLKPDDITSIPNVD
                     LVLGANDKFNIPDIIAKKTAMPNKLQASPTNRMTEFHHSYSSEDRTRHFLKVQDGCDY
                     FCSYCTIPLARGRSRNGSIENLVKDAQSIAALGGKEIVLTGVNIGDFGHSTNESFIDL
                     LKALEQVEGIERYRISSIEPNLLTDDIIALVAQSTKLMPHFHIPLQSGCNEVLNLMKR
                     RYTTELFAQKIATIKQLMPHAFIGVDVIVGVRGETTSYFETTYQFIESLPISQLHVFS
                     YSERAGTQALKIEHVVTPNEKKRRSHLLLSLSEQKTEAFYTNFKHTIRPVLWEGTKKG
                     GFMYGFTDNYIRVKTPYVIHKINTIESIILTAETISIEKS"
     gene            complement(2775..2918)
                     /locus_tag="PLJ82_06805"
     CDS             complement(2775..2918)
                     /locus_tag="PLJ82_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOR41248.1"
                     /translation="MDKHIPVAIYCRAGRRSKIAAEKMSAIGLQGYELDNGIVSWENE
                     LTR"
     gene            complement(2924..3148)
                     /locus_tag="PLJ82_06810"
     CDS             complement(2924..3148)
                     /locus_tag="PLJ82_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOR41249.1"
                     /translation="MTTRLLILSILIFTITSCTSQTSSFKSVDAKEFEQILRTDTLQI
                     VDVRTPEEFNQGHIQGAICMDVRNPDFDSK"
     gene            complement(3244..3963)
                     /locus_tag="PLJ82_06815"
     CDS             complement(3244..3963)
                     /locus_tag="PLJ82_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOR41250.1"
                     /translation="MARNSFFEFKQFRINQENCAMKVGTDGVLLGAWTPIPLSTTRIL
                     DIGTGSGLLALMLAQRTHAHIDAVEIDTLAAQQAKENFISSPWSNRLQIYETDFQSFA
                     KNELHKYDVIVSNPPYFHNSLHTPNRQRTIARHSDSLTLDELVDGVEKLLQPWGYFCV
                     ILPTHESETVAKKALLANLYCTQKTFVYPTPTSPAKRIMQLFSKKETTPLTQHLVIEQ
                     EQRHQYTKEYSELTKDYYLNF"
     gene            4174..5244
                     /gene="carA"
                     /locus_tag="PLJ82_06820"
     CDS             4174..5244
                     /gene="carA"
                     /locus_tag="PLJ82_06820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOR41251.1"
                     /translation="MQKSKAAKLVLENGVVFHGYSFGYEQSKAGEVVFNTAMMGYPES
                     LTDPSYSGLILAFTFPLIGNYGVPSSNVTHQIADFFESEKIHASGVVVSTYSFEYSHW
                     NAKQTLSDWLIENKVPGIYGVDTRELTKVLREKGAMKGKIVIEDADVDFIDSNAINQV
                     NIVSCKEVMHYGNGKKKVVLVDCGVKHNIIRCLLKRDVTLIRVPWNYDFNTLEFDGLF
                     LSNGPGDPSTCAETVEHIRKAMKRNVPIFGICMGNHLLAKAGGAETYKLKYGHRSHNQ
                     PVRMVGTNRCFVTSQNHGYAVDNTTLSEEWEPLFQNMNDDTNEGLKHKSKPFFSVQFH
                     PEAASGPTDTEFLFDEFIKMID"
     gene            5244..>7118
                     /gene="carB"
                     /locus_tag="PLJ82_06825"
     CDS             5244..>7118
                     /gene="carB"
                     /locus_tag="PLJ82_06825"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOR41252.1"
                     /translation="MNENLIPGIKKVIVLGSGALKIGEAGEFDYSGSQALKALKEEGI
                     YTVLVNPNIATIQTSEGVADQIYFLPITPFFVEKVIQKEKPDGILLAFGGQTALNCGV
                     QLHRLGILEKYNLRVLGTPVQAIMDTEDRDLFVKKLDQIDVKTIKSFAVDNMEDARKA
                     AKDLGYPIIIRAAYALGGLGSGFCDNEEELDAMAEKSLSFSPQILVEKSLKGWKEVEY
                     EVVRDRFDNCITVCNMENFDPLGIHTGESIVIAPSQTLTNAEYHKLRELAIRIIRHIG
                     IVGECNVQYALDPESEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFDV
                     KNSVTKTTPAFFEPALDYVVCKIPHWDLGKFQGVSRELGSSMKSVGEIMAIGRTFEEA
                     VQKGLRMIGQGMHGFVANKELVIADIDKSLNEPTDNRIFVISKAFRKGYTIDQIHDLT
                     KIDKWFLQRLYKIVETANELEAFNSVEDVDDELFRLAKQQGFSDFQIARLTSDSAESE
                     ESLIKVRNNRKARGITPVVKRIDTLAAEYPAQTNYLYLTYNGTANDMQYLGDKRSIIV
                     LGSGAYRIGSSVEFDWCSVNALETIRKEGWRSVMINYNPETVSTDYDTCDRLYFDELS
                     F"
BASE COUNT         2141 a         1251 c         1385 g         2341 t
ORIGIN      
        1 aaataagatg gaagtgtgtt ttcattcaat taataggtat tattgataaa tgttaaagat
       61 agctttgtat ctgtgtgttt aatcctctga aaatatactc tttttatagt gtcaaaagta
      121 ctatttagtc tttaaaataa caaaaatatt ttataaacgt tgtgatacct gttgtatcat
      181 gtttgttttc catactgaaa aagtgcctgc aagtatttgt ttgcgtgcct cttgcactaa
      241 ccataaataa aaagctagat tgtgaatgga tgcaatttgc aatcctaaca attcgttgga
      301 tataaacaaa tgacgcaaat aggctttaga gtaggcggta tctacatacg atgctccatc
      361 tatttcgata ggagaaaaat cgtctgccca ttttttatta cgcatattta taattccatt
      421 ttttgtaaaa agcataccgt ttctgccatt gcgtgtaggc attacacagt caaacatatc
      481 tacccctcgt tcaatagctt ccaataggtt ggcaggtgta ccaacaccca ttaggtaacg
      541 aggtttatct gtaggcagta tctcgtttac tatttcaatc atttcataca ttttttcggt
      601 aggttcgcct actgccaagc ctccaatagc atttccttct ctgccttgcg aggctacaaa
      661 ttctgccgac tgtttgcgta aatctggata tacgcatcct tgcacaatgg gaaaaaatgt
      721 ttggctatac ccatatttcg gttcagtagt gtcaaaatgt tgtatgccac gtgccaacca
      781 tcgatgcgta agttccattg atttttttgc atacgcataa tcggcatcac ctggggtaca
      841 ttcgtcaaga gccataataa tgtctgctcc gataatacgc tgaatatcta ccacattttc
      901 gggcgtaaac aaatgttttg aaccatcaat atgcgaatga aaggttacgc cctcttcttt
      961 catttttcgt ctatgcgaaa gtgaaaatac ctgaaaacca ccactatccg taagtatagg
     1021 cttgtcccat ccgttaaatt tatgtaagcc tcctgctttt tccagaatct cgatacctgg
     1081 tcggaggtat aaatggtagg tgtttcctaa gatgatttgt gcttttatat cctctttcaa
     1141 ctcgtgttga tgtactgctt ttactgatgc tactgtacct acgggcataa aaatgggtgt
     1201 ttctatttgt ccatggtcgg ttgttattaa gcctgctcga gcattacttt tggggtcggt
     1261 atgttgtagt tcaaatgtca tttcattaac ttttttctat gctaatcgtt tctgcggtta
     1321 atataataga ctcaatcgta ttaattttat gtattacgta aggcgttttt acacgaatat
     1381 agttatctgt aaatccatac ataaaaccgc cttttttagt gccttcccat aaaacgggtc
     1441 ggatggtatg cttaaagtta gtgtaaaaag cctctgtctt ttgctcggat aacgagagta
     1501 ataagtggct ccgtcttttt ttttcgttgg gtgttaccac atgctcaatt tttaatgctt
     1561 gtgtgcctgc tctttccgaa tacgaaaaca catgcaattg tgaaataggt aaagattcaa
     1621 tgaattggta agttgtttca aaataggatg tggtttctcc tcgtacgcct acaattacat
     1681 ctacaccaat aaatgcgtgt ggcatcagtt gttttatagt ggcaattttt tgagcaaaca
     1741 attcggttgt gtagcgtctt ttcattaagt tcaatacctc attacagccc gactgtagtg
     1801 gaatatgaaa atgaggcatt agtttggtag attgtgccac tagagcaata atatcatctg
     1861 ttaataaatt tggttcaata gaagatatcc tataacgttc tattccttct acttgttcaa
     1921 gtgcctttaa taaatcaata aatgattcgt ttgttgaatg tccaaaatca ccaatattga
     1981 cgcccgttaa tactatttct ttaccaccaa gtgctgcaat gctttgtgca tcttttacta
     2041 aattttcgat agagccattt ctacttctgc ctctagccaa tggaatagta cagtagctac
     2101 aaaaataatc acacccatct tgtactttta aaaaatgtct tgtcctgtct tcgctcgaat
     2161 atgaatgatg aaattcagtc attcggttgg tagggctagc ttgcaatttg ttgggcatag
     2221 cagtcttctt tgctataata tcaggaatat tgaatttatc gtttgctccc aacaccaaat
     2281 caacattggg tatagaagta atatcatcgg gtttgagttg agcataacaa cctgtaacaa
     2341 taacaaaagc tcctgggttt tctttaatgg ctttggtaat agtttgtcta catttcttat
     2401 cagcactatc tgttaccgag caggtgttaa taatacaaag cgaggctggc tcgtgagggg
     2461 ctactttgcg atacccttgc tcaatcaaac ttttacctat agcagatgtt tccgaaaaat
     2521 tcagtttgca acctagtgta taaaaagcta ccgttttatt gtcaaaaaca gatgtgtcaa
     2581 tcataattat atgtacatgg aataattcaa aatcaggatg caaaagtact atttttttgc
     2641 tgtattgcat ctatttgttg ttataaatga atattcctta acgttttata gctatgttat
     2701 ttaggtggat atataccaga tagaggtatt atccgtatag ggatgattcg ttttattgtc
     2761 cttttacaaa atagttaacg agttaattca ttttcccaag acacaatgcc gttatctagc
     2821 tcatatcctt gtagtccaat tgcactcatt ttttcagcag caattttact tcttctccca
     2881 gctctgcaat agatagccac agggatatgt ttatccaatt gtattatttt gagtcaaaat
     2941 ctggatttct aacatccatg caaatagccc cttgaatatg tccttgatta aattcttcag
     3001 gtgtgcgtac atccacaatt tgaagagtat ctgtacgtaa tatttgctca aactcttttg
     3061 catcaactga tttaaaagaa cttgtttgac tggtgcaact tgtgatggtg aaaataagta
     3121 tacttaaaat aagtaaacgg gttgtcatat ttttttatgt tactgtatgc ataattacca
     3181 caaatgtaca cataattaat aaacttggaa tgatattttt ttttgaatta gtgtttttcc
     3241 tcttcaaaaa tttagataat aatcttttgt aagttcacta tattctttgg tatactgatg
     3301 cctttgttct tgttcaatta ccaaatgctg tgtaagcggg gttgtttctt ttttcgaaaa
     3361 tagttgcatg atgcgtttgg ctgggctcgt aggtgttggg taaacaaacg ttttttgtgt
     3421 gcaataaaga tttgccagca aagccttttt agctactgtt tcactttcat gtgtaggtaa
     3481 tatgacacaa aaatatcccc aaggttgtag taatttttca acgccatcaa ctaattcgtc
     3541 tagcgtaagc gaatcactat gtcgagctat tgtacgttgt ctgtttggag tatgcaatga
     3601 attgtgaaaa taagggggat ttgatacaat aacatcatat ttatgtaatt cgtttttggc
     3661 aaatgattga aaatcggttt cataaatctg taatcgattg ctccaaggag aagatatgaa
     3721 attttctttg gcttgttgag ctgccaaggt gtctatttct acggcatcaa tatgggcatg
     3781 tgtgcgttga gcgagcatca gagcaagtaa tccacttcct gttccaatgt ccaatatacg
     3841 ggtagtgcta agtggtatgg gagtccatgc cccaagcagc acaccatctg tgccaacttt
     3901 catagcacaa ttttcttggt taattctaaa ttgtttaaac tcaaaaaacg aatttcttgc
     3961 cataaatatg ttaaaaatgc aaatatacag taaacttttc atatgctaaa cagtgtctaa
     4021 ctcccatcaa tatggctatc gaacaaatca atttattatt cgtgtaaaaa tgttgaaaaa
     4081 tagcaaaatc ctgtttgttt ggaatgctaa aatattgtat ctttgtgcaa tttttgagaa
     4141 ctactcattg taacatattt atattagtaa aacatgcaaa aaagcaaagc tgccaagttg
     4201 gtgttagaga atggagtcgt ttttcatggg tactccttcg gttatgaaca gtcgaaagca
     4261 ggagaggtgg tttttaacac tgccatgatg ggctatcccg aaagtttaac agacccttcc
     4321 tattcgggtc taatcttagc atttacattc cctttaatcg gcaattatgg tgttccttct
     4381 tccaatgtaa cccatcaaat tgccgatttt tttgagtccg aaaaaattca tgcttcgggc
     4441 gtagtagtgt caacatattc gtttgaatac agccactgga atgcaaaaca aaccctaagc
     4501 gattggttga ttgaaaacaa agtgccaggg atttatggcg ttgatacgcg tgaattgaca
     4561 aaagtattgc gcgaaaaagg tgcaatgaaa ggaaaaatcg ttatcgaaga tgctgatgtt
     4621 gattttatcg attcaaatgc aattaatcag gtaaacatcg tgtcgtgcaa agaggtaatg
     4681 cactacggaa atggaaagaa aaaagtagta ttggtcgatt gtggtgtaaa acacaatatc
     4741 attcgttgct tattaaaaag agatgtaacg ctcattcgtg tgccttggaa ttatgatttt
     4801 aataccctcg aatttgatgg attatttttg tctaatggtc ctggcgaccc atctacatgt
     4861 gctgaaactg tagaacatat acgcaaagca atgaaacgaa atgtacccat atttggtatt
     4921 tgcatgggca atcatttgtt ggcaaaagcg ggtggtgctg aaacatataa actaaaatat
     4981 ggtcatcgta gccacaacca acccgtgcgt atggtgggaa caaatcgttg ttttgtaact
     5041 tcacaaaatc atggatatgc tgttgataac actaccttga gtgaagaatg ggaacctctt
     5101 ttccaaaata tgaatgatga taccaacgaa gggctaaaac ataaatcaaa acctttcttt
     5161 tcagtccaat ttcaccccga agcagcaagt ggacctactg atacagaatt tttatttgac
     5221 gaatttatta aaatgattga ctaatgaacg agaatttaat accaggaata aaaaaagtta
     5281 ttgttttagg ctcaggagca ctcaaaattg gggaagctgg cgaatttgat tattcaggct
     5341 ctcaagctct gaaagcacta aaagaagaag gaatttacac cgttttggtc aatccaaata
     5401 ttgctaccat tcaaacatcc gaaggggtag ctgaccagat ttatttctta cccattacgc
     5461 cattttttgt cgaaaaagtc atccaaaaag aaaaacctga tggtattttg ttggcttttg
     5521 gtggtcaaac agctcttaac tgtggtgtac aattgcaccg attaggaatt ttagaaaaat
     5581 acaatttgcg tgtattggga actcccgtac aggcaattat ggatacagaa gaccgcgatt
     5641 tgttcgtgaa aaaactcgac caaattgatg taaaaacaat caaaagtttt gctgtagata
     5701 atatggaaga tgctcgcaaa gctgctaaag atttggggta tccaattatt atacgtgcgg
     5761 cttatgcctt aggtggttta ggtagtggat tttgtgataa tgaagaggaa ttagatgcaa
     5821 tggctgaaaa atcattgtct ttctctcccc aaatattagt cgaaaaatca ttaaaagggt
     5881 ggaaagaggt tgagtacgag gtagttcgcg accgttttga caactgtatt acggtatgta
     5941 atatggagaa tttcgacccg ctcggtattc atacgggcga aagtatcgtt attgctcctt
     6001 cgcaaacgct tacgaatgcc gaataccaca aattgcgtga attggcaatc cgaatcattc
     6061 gccatatcgg tatagtgggt gagtgtaatg tgcaatatgc tctcgaccca gaatcggaag
     6121 attatcgtgt aattgaggta aatgcccgtt taagtcgctc ttcggctttg gcttctaaag
     6181 ctacaggcta tccattggct tttgtagctg caaaattggg attggggtat gggttgttcg
     6241 atgtcaaaaa ttcagtaact aaaactactc ctgcgttttt tgaacctgca ctcgactatg
     6301 tggtgtgtaa aattcctcat tgggatttgg gcaagtttca aggcgtttcg cgcgagttag
     6361 gctcaagcat gaaatcagta ggcgaaatca tggctatcgg acgtacgttt gaagaagctg
     6421 tgcaaaaagg cttgcgtatg attggtcaag gaatgcacgg ttttgttgcc aataaagaac
     6481 tggtaattgc tgatattgac aaatcgctaa acgaaccaac agataaccgt atttttgtta
     6541 tctcaaaagc atttcgtaaa gggtatacca tcgaccaaat tcatgattta accaaaatag
     6601 ataaatggtt tttacaacga ttatataaaa ttgttgaaac agccaacgaa ttggaagcat
     6661 tcaattcggt agaagatgtt gatgatgaat tattccgttt ggctaaacaa caaggctttt
     6721 ctgacttcca aatagcacgt cttacaagcg atagtgctga gagtgaagaa agtttgataa
     6781 aagtaagaaa taaccgcaaa gctagaggga ttactcctgt agtgaaacgt attgatacct
     6841 tagccgctga gtatccagct caaacaaatt acttgtattt aacctacaac ggcacagcca
     6901 acgatatgca gtatttgggc gataaacgct ctattattgt acttggctct ggagcatacc
     6961 gcattggtag ctcggtagag ttcgactggt gtagtgtaaa tgccctagaa actatacgca
     7021 aagaaggttg gcgttcggta atgataaatt ataaccccga aacggtttct acggattatg
     7081 atacttgcga ccgtttatat ttcgacgaat tatcgttt
//
LOCUS       DAOSRP010000100         7036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9941_length_7036_cov_4.688440, whole genome shotgun sequence.
ACCESSION   DAOSRP010000100 DAOSRP010000000
VERSION     DAOSRP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_7036_cov_4.688440"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PLJ82_06830"
     CDS             <1..1371
                     /locus_tag="PLJ82_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOR41253.1"
                     /translation="FDELSFERVMDIVELENPHGLIVSTGGQIPNNLALRLNNMNVPL
                     LGTTATSIDRAEDRHKFSSMLDSLKIDQPRWKELSSLEDVQLFVDEVGFPVLVRPSYV
                     LSGAAMNVCSNKEELKEFLQLAANVSKKHPVVVSEFIESAKEIEMDAVADKGEIMMYA
                     ISEHIEFAGIHSGDATIQFPPQKLYLETMRRVKKIAHQIAKELCISGPFNIQFLAKNN
                     EIKVIECNLRASRSFPFVSKVLKINFIELATKIMLGIPVQKPNKNAFNLDYVGIKASQ
                     FSFSRLQKADPVLGVDMASTGEVGCIGDDADEAILTSMLSVGYTIPKKNILLSTGTPK
                     QKVDMLDAARLLLKKGFNLYATGGTHTFLQENDIPATLVHWPSEEMQPQALEMLKSKQ
                     IDLVVNIPKNLTQRELDNGYKLRRAAIDFNIPLLTNARLASAFIHAFCHLDINDVQIK
                     SWNEYK"
     gene            1400..3277
                     /locus_tag="PLJ82_06835"
     CDS             1400..3277
                     /locus_tag="PLJ82_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOR41254.1"
                     /translation="MEQLKHECGIAMIRLRKPLDFYQQKYGTWMYGLNKLYLLMEKQH
                     NRGQEGAGVACVKLDAHPGEEFMFRERAEGTSAISKIFADMHKQIREVANGDIDDVEY
                     ARQFVPFAGELYMGHLRYSTTGKAGISYVHPFMRRDNYRAKNLAICGNFNLTNVDEIF
                     NYISKKGQHPRKYADAFILLEQIGHRLDREVERVYALAKEEGKRGTDITYAIEEGIKI
                     ENVLQSSSWLWDGGYVMCGLTGSGESFVLRDPWGIRPAFYYADEEIVVTASERPVIQT
                     ALNLTASQVKELNPGEAMILDKAGNLRLVQVLQPKIKQACSFERIYFSRGSDCDIYNE
                     RKALGENVVPGILKSIDNDLKNTVFSFIPNTSEVAFYGMIQCLNERLNDIKNQQIRQY
                     GRRLTSEMLDEILSMSIRSEKVAIKDIKLRTFISEGNSRNELAAHVYDVTYGSIVPNQ
                     DNLVVIDDSIVRGTTLKQSIIKILARLQPKKIVIVSSSPQVRYPDFYGIDMSNMDEFV
                     AFRAAIALLKDNNMQHVIDDVYKKCKQQENQHKEEIENYVKQIYAPFTAEQVSEKIVE
                     LLKPEDVQCPVELVYQSLEGLLKSCPNHTGNWYFSGDYPTQGGKRLVNESFIRYYEKN
                     Y"
     gene            complement(3337..3810)
                     /locus_tag="PLJ82_06840"
     CDS             complement(3337..3810)
                     /locus_tag="PLJ82_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HOR41255.1"
                     /translation="MYTWFECKVRYEKIVEGKTKKVTEPYLIEAFSYTEAEARVMQEL
                     SPYISGDYSITNIKRAKIAELFPNDLGDRWYTCKINIILLDESRGIEKKVANYILVQA
                     SAVLDAWQKLEIGMKGTLSDYVVVSIGESPIIDIFPYKKEEMKQVISSTNVSIEE"
     gene            4062..5063
                     /gene="gap"
                     /locus_tag="PLJ82_06845"
     CDS             4062..5063
                     /gene="gap"
                     /locus_tag="PLJ82_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOR41256.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIQVVAVNDPFLDLDYLVYM
                     LQYDTVHGKFKGTAENKDGKLIVNGKEVKFYAEKDPAAIAWGAAGADYVVESTGVFTT
                     IDKAKAHLAGGAKKVVISAPSNDAPMFVCGVNLDAYKADMDIVSNASCTTNCLAPLAK
                     VINDNFGIVEGLMTTVHAATNTQKTVDAPSAKDWRGGRSILGNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMAFRVPTADVSVVDLTVRLEKAASYDEIKATIKKASENELKGILGYTE
                     DSVVSTDFTTDSRTSIFDAGAGIALNGNFVKLVSWYDNEWGYSNKVLDLIAHMDSVK"
     gene            5271..6269
                     /locus_tag="PLJ82_06850"
     CDS             5271..6269
                     /locus_tag="PLJ82_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41257.1"
                     /translation="MKKVLIILLSLIAMVWSVNAAPGELTTIPSNPTVNNSITLIYEP
                     LPNQSWMLSQDVFIYTCLEFDENGEWVKQKAEWSKTSLPNFKWVKEDDGKLYYIISNI
                     KTFFNLTDDEVSRVSGIFVILKNEKFQSTDKYVAIRGERKPVEKFTGTVSFEVKVPEG
                     TKSVYVAGTFGPEGTPNFWKHADEKYKLTRKDATTFVGVIKDVPEDLQYLYVYGSRVD
                     QAEFRMGHRPLGGKTKVHDVVEYWGDMTLNVSVPKGTKEVYVSGNFNNWGHSLMTDIG
                     NNTWIYHVAPVKIGADEQVEYKYYTKADKTASEPQQNNRKLKFVGFSTQYDEVTKW"
     gene            6353..>7036
                     /locus_tag="PLJ82_06855"
     CDS             6353..>7036
                     /locus_tag="PLJ82_06855"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HOR41258.1"
                     /translation="MKKILLSLVALFATVSLVSALTYNVTVPAGTNECYIAGEFNGWS
                     QAKMTQQTATTYTITIAEATATSKYKYCSGPSWAFVEQTAGGGEVTDRTYVPEGDIVE
                     KWAAVYDPNAGGSGDIVIKAKKPASWSAMSIHFWGDASSDWPGEAMTQEGDWYVKTFS
                     SVTAISIIFTDGVNTGTRQTGNINALTASTCYEVSDDGTTVSYQVVTCPVTGIQSTFD
                     AAVVAANGTI"
BASE COUNT         2322 a         1108 c         1400 g         2206 t
ORIGIN      
        1 ttcgacgaat tatcgttcga acgtgtaatg gatatcgtag aattagaaaa tccacatgga
       61 ttaatcgttt ctacaggtgg tcaaattcca aataacttag ctttacgttt aaataacatg
      121 aacgtgccat tgttgggtac aacagccacc agtattgata gagctgagga tagacataag
      181 ttctcgtcga tgttggatag tttaaaaatt gaccagccac gttggaaaga gttgtcttcg
      241 ctcgaagatg tacaactttt tgtcgacgaa gtaggtttcc ctgtgcttgt tcgtccatcg
      301 tatgtgcttt ctggagcagc aatgaatgtg tgttctaaca aagaagaact gaaagagttt
      361 ttgcaattag ctgctaatgt gtctaagaaa caccctgtgg tagtaagcga atttatcgaa
      421 agtgccaaag aaatcgagat ggatgcggta gccgataaag gggaaataat gatgtatgcc
      481 atttccgaac acatcgagtt tgctggtata cactctggtg atgctactat tcagtttcct
      541 cctcaaaaat tgtacttaga aacaatgaga agggtgaaaa aaatcgctca ccaaattgcg
      601 aaagaactat gtatctcagg tccatttaat attcaatttt tagctaaaaa taacgagatt
      661 aaggttatcg aatgtaattt gagggcttct cgtagttttc catttgtatc taaagtgcta
      721 aaaattaact ttattgaact agcaaccaaa attatgttgg gcatacctgt tcaaaaaccg
      781 aacaaaaatg cctttaactt agattacgta ggtatcaaag cttctcaatt ctctttctct
      841 cgtttgcaaa aagctgaccc agtattgggt gtggatatgg cttcaacagg cgaagtggga
      901 tgtattggcg atgatgctga tgaggcaata ttaacttcta tgctatctgt aggttatact
      961 attccgaaaa agaatatttt attatcaaca ggtacaccaa aacaaaaagt agatatgctt
     1021 gatgcagctc gtttgttgct taaaaaaggc tttaatctat atgcaacggg tggtacacac
     1081 accttcttgc aagaaaatga tatacctgct accttggtgc attggcccag cgaagaaatg
     1141 caaccacaag ctcttgaaat gctcaaaagc aaacagattg acttggtggt aaatatccct
     1201 aagaatttaa cgcaaagaga actagataac ggatataaac ttcgtcgtgc agccattgat
     1261 tttaatattc cactattaac aaacgctcgt ttagcctctg catttataca tgcattttgt
     1321 catttagata tcaatgatgt acaaattaaa agctggaacg aatacaaata attgatcttt
     1381 cgaccctaac ataaccacaa tggaacaact aaagcatgaa tgcggtatag cgatgattcg
     1441 cctgcgtaag cccctggatt tttaccaaca aaaatacggc acgtggatgt atggcttaaa
     1501 caagctttac ttattgatgg agaaacaaca caatcgcggg caagaaggtg ctggtgtagc
     1561 atgtgtcaaa ttagatgctc atcctggtga ggaatttatg tttcgcgaac gtgccgaggg
     1621 tacaagtgca ataagcaaga tatttgctga tatgcataag cagattaggg aagttgccaa
     1681 tggcgatatt gatgacgtag aatatgctcg tcagtttgtg ccttttgctg gcgaattata
     1741 tatggggcac ctccgttata gtaccacggg taaagctgga atttcgtatg tacatccttt
     1801 tatgagaaga gataattaca gagcaaagaa tttagctatt tgtggtaatt ttaacttgac
     1861 caatgtagat gaaattttta attacatatc taaaaaaggg caacatccgc gtaaatatgc
     1921 cgatgcgttt attctattgg agcaaattgg gcatcgcctt gatcgtgaag tagaacgtgt
     1981 atacgcctta gccaaagaag aaggtaaaag aggtactgat ataacatatg ctattgaaga
     2041 aggtattaag attgaaaatg ttttacagtc atcttcttgg ctgtgggatg gtggatatgt
     2101 tatgtgtggt ttaacaggaa gtggcgaaag ttttgtgctt cgggatccat ggggaattcg
     2161 ccccgccttc tattatgctg atgaggaaat tgtagttact gcctctgaaa gacctgtaat
     2221 acaaacagca ttaaatctta cggcaagtca ggtaaaggaa ctaaacccag gagaagcaat
     2281 gatcctcgat aaagcaggaa acttgcgact agtgcaagta ttgcaaccca aaatcaaaca
     2341 agcatgttcg ttcgaacgta tttatttttc gcgtggtagc gattgtgata tttacaacga
     2401 acgcaaagcg ttgggtgaaa atgtagtgcc aggtattctt aaaagtattg ataatgattt
     2461 aaaaaatact gttttttcat ttattcccaa cacttctgag gttgcttttt atggaatgat
     2521 tcaatgtctt aacgaacgtt tgaatgatat taaaaatcaa caaatacgtc agtacggacg
     2581 acggttgact tctgaaatgt tagatgaaat attatccatg tctattcgtt cagaaaaagt
     2641 agcaataaaa gatatcaagt tgcgtacgtt tatttccgaa ggcaatagtc gcaatgagtt
     2701 agctgctcac gtgtacgatg ttacgtatgg tagcattgtg cccaaccaag ataatttagt
     2761 cgttattgat gatagtattg tgcgtggtac tacccttaaa caaagtatca ttaaaatcct
     2821 agcacgattg caaccaaaaa agattgtaat tgtatcatca tcaccacaag ttcgttatcc
     2881 tgatttttat ggtatagaca tgtcgaatat ggacgagttt gtagcttttc gtgcagcgat
     2941 tgcattgctc aaagacaata acatgcaaca tgtcattgat gatgtgtaca aaaaatgtaa
     3001 gcaacaagaa aatcagcata aggaagaaat agagaattac gttaagcaaa tatatgcccc
     3061 ttttacagca gaacaagttt ctgaaaaaat tgtagaatta ctcaaacccg aagatgtgca
     3121 atgcccagtg gaattggtat accaaagttt agagggcttg ttgaaatcat gtccgaatca
     3181 tacaggtaat tggtattttt ctggagatta tcctacacaa ggaggtaaac gtttagtcaa
     3241 tgaatcgttt attcgatact acgaaaaaaa ttattaaata aaaatagtcc tcgatgttat
     3301 atcgaggact atttttatta ctattgtata gctatcttat tcttctattg atacattcgt
     3361 cgaactaatt acttgtttca tttcctcttt tttatacggg aaaatatcaa ttattggact
     3421 ctctcctatc gatacaacca cataatcaga taaggtgcct ttcatgccta tttctaattt
     3481 ttgccaagca tctaacacgg cagaggcttg taccaatatg taattagcta ctttcttttc
     3541 tatacctcta ctttcatcaa gcaaaatgat attaatttta cacgtatacc atctgtcgcc
     3601 caaatcgttt ggaaaaagtt ctgcgatttt ggcacgttta atgtttgtga ttgaataatc
     3661 cccacttata tatggcgata attcttgcat tacgcgagct tccgcttctg tgtacgaaaa
     3721 agcttcaatt aaataaggtt ctgttacttt tttagttttt ccttctacta ttttctcata
     3781 gcgtacttta cactcaaacc atgtatacat attattgttg tttttaatta gagtgcaaaa
     3841 atacagataa taatgagcct caaaagaaaa ttaggaaaat agttttccac aaaattgcag
     3901 ttacttgttt acaagcctaa tagcatttat taataaacca aatatgggtt aacaaatctc
     3961 tatttttttt gaaaaaaaac aaacaattct ttggttagta ataaaaaaaa aagtaatttt
     4021 gcatggttta aaaaaataac ttaatttata aataagaaaa aatgattaaa gtaggtatta
     4081 atggatttgg ccgtatcgga cgtttagtgt tccgtgcagc tcaagaaaga aacgacattc
     4141 aagttgttgc agtaaatgac ccatttttgg atcttgacta tttagtttat atgttgcagt
     4201 atgatactgt acacggtaaa tttaaaggta cagctgaaaa taaagatggt aagttaatcg
     4261 taaacggtaa agaagtaaaa ttctatgccg aaaaagatcc tgctgctatt gcttggggtg
     4321 ctgctggtgc tgattatgtt gttgaatcaa caggtgtttt cactactatc gacaaagcaa
     4381 aagctcactt agcaggtggt gctaaaaaag tagttatctc tgctccttcg aatgatgctc
     4441 ctatgtttgt gtgtggtgtt aacttagatg catacaaagc tgatatggat atcgtatcaa
     4501 atgcatcttg tactactaac tgtttggctc ctttggctaa agttattaat gataattttg
     4561 gtattgtaga aggtttgatg acaactgtac acgctgctac taatactcaa aaaacagttg
     4621 atgctccttc tgctaaagat tggagaggtg gtcgttcaat cttaggtaat attatcccat
     4681 cttctacagg tgctgcaaaa gcagtaggta aagttatccc atcattgaat ggtaaactta
     4741 caggtatggc tttccgtgta ccaacagctg acgtttctgt agttgactta actgtacgtt
     4801 tagaaaaagc tgcttcgtac gacgaaatta aagctactat caaaaaagct tctgaaaatg
     4861 aattgaaagg tattttaggg tatactgaag actcagtagt ttctactgac tttactaccg
     4921 actctcgtac ttcaattttt gatgcaggtg caggtattgc tcttaatggt aacttcgtga
     4981 aattagttag ctggtatgac aatgagtggg gttattctaa caaagtgtta gatttaattg
     5041 cacacatgga ttcagttaaa taacactaat ttttttagta aatgcaattg ggtagtaaat
     5101 taatttacca cccaattttt tttgctatag ggataaaaac atccataaaa tgttaaaaat
     5161 cttttttttg agtaaaaaaa agtgtgaaaa tagagttaat aacaaaaaaa gttgtatatt
     5221 tggaacgtct ttttgttgaa aagaaaaaca ccttaaaaaa aattaatgat atgaagaaag
     5281 ttttaattat tcttttaagc ctgatagcca tggtttggag cgtgaatgct gcacctggcg
     5341 aattaacgac gataccgtcg aatccgacag taaataactc aatcactctt atctacgagc
     5401 ctcttccaaa tcaaagttgg atgctatcac aagatgtttt tatttatacg tgtttagagt
     5461 ttgatgagaa tggggagtgg gtaaaacaaa aagctgagtg gagtaaaacg agtttgccta
     5521 attttaaatg ggtaaaagag gatgatggaa aattgtatta tattatatca aatatcaaaa
     5581 cattttttaa tttaacggat gatgaagttt ctagagtttc tggtattttt gtgattttga
     5641 aaaatgagaa atttcaatca acagacaagt atgtagccat aagaggagaa agaaaacctg
     5701 tagaaaaatt tactggaact gtttcttttg aggtaaaagt acctgaagga acaaaaagtg
     5761 tatacgtggc tggaacattt ggaccagaag gaacacccaa cttttggaaa catgctgatg
     5821 aaaaatataa gttaacaaga aaagatgcta ctacatttgt cggtgttatc aaggatgtgc
     5881 cagaggattt acaatatttg tatgtgtatg gttcaagagt agatcaagct gaatttcgta
     5941 tgggacaccg cccattagga ggcaaaacaa aagtacacga tgtggttgag tattggggag
     6001 atatgacatt gaatgtgtct gtgccaaaag gaactaaaga agtgtatgtt tctggtaatt
     6061 ttaataactg gggacatagt ttgatgactg atataggtaa taatacatgg atttatcatg
     6121 tagctcctgt aaaaataggt gctgacgagc aggtggaata caaatattat accaaagcag
     6181 ataaaacggc aagcgagcca caacaaaata accgtaaatt gaaatttgta gggttctcta
     6241 cacaatacga tgaggttacc aaatggtaag tgttaatgta catataattt aaaacctaat
     6301 tatatttatt cttttattct ttaactattt attaattaaa aattttacaa caatgaaaaa
     6361 gattttactt tctttagtag ctctatttgc tactgttagt ttggtaagtg cccttactta
     6421 caatgttact gtacctgctg gtacaaatga gtgttatatt gcaggagagt ttaatggttg
     6481 gtctcaagca aaaatgacac agcaaactgc aactacctat actattacaa ttgccgaagc
     6541 tactgcaaca tcaaaataca aatattgcag tggtccaagt tgggcttttg tagaacaaac
     6601 tgctggtggt ggtgaagtta ctgatcgtac ttatgttcct gaaggagaca tcgtagaaaa
     6661 atgggctgct gtttatgatc ccaatgctgg aggtagtggc gacattgtta tcaaagctaa
     6721 aaagccagct tcttggagtg caatgagtat tcacttttgg ggagatgcta gttctgattg
     6781 gccaggagaa gctatgactc aagaagggga ctggtatgtt aaaacattta gtagtgtgac
     6841 tgctatttca attatcttta ctgatggtgt aaatactggc acaagacaaa caggaaatat
     6901 taatgcttta actgcttcta catgttacga agtaagtgat gatggtacta ctgtatctta
     6961 tcaagttgtt acttgtccag ttacaggtat ccaatctaca tttgatgctg ctgtagtagc
     7021 tgcaaacgga acaatt
//
LOCUS       DAOSRP010000101         7023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_9967_length_7023_cov_3.412026, whole genome shotgun sequence.
ACCESSION   DAOSRP010000101 DAOSRP010000000
VERSION     DAOSRP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_7023_cov_3.412026"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLJ82_06860"
     CDS             <1..885
                     /locus_tag="PLJ82_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOR41259.1"
                     /translation="GLTPGINTMRWTLRGYGCTAYDEMQLRYAEEPIADFEPDVISGC
                     SPLTVGFANTTIGQATYNWDFGDGKTSTQRSPEHTFERSGLYEVTLTAVGLRKTDKIT
                     KLIEVLPSPKAAFLVANTELFMPYPMANFYSNTPEAIKYWWRFGDGATSTEKDPTHLY
                     QLEGIYDVWLKVEDEKGCQDSIMVYNHIHVGDYGMMVFPNAFTPNTSIPNGGVYQAEE
                     RRLDIFYPVSRNVDTYKLEIFNQWGAKVFVSNDIFVGWDGYYKGESAPQGVYMYKATG
                     SYKNGRKFTVSGEFILVR"
     gene            909..1895
                     /locus_tag="PLJ82_06865"
     CDS             909..1895
                     /locus_tag="PLJ82_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOR41260.1"
                     /translation="MKYQLIAIICFFVLFVSPKKVVAQDFLVSQPWSTNTTLGPSFAG
                     LTGSRAFMAYRDQWSFMRGSQTLLAGFDHYYHPIQSSFGAVVAHSSYGGNLLTQTELA
                     FQYNYVAKITDEWLFRPGIEFGPYYRAIDPSKFMFIDQIALDGTILPTSSFEPDETSV
                     LRFDAAVSFLLYNEFFFIGAGADRLMSNDVSFANTNTKTDLKFNAYMAGRIPMNRGMG
                     KYASYDDVTIAGLFQQQGLYQQMDLDVLYHRIPFELGIGYRGIPFVSPQGLSNTDALK
                     LIFGYSFGDIVVSYSYDVSISSLFNVSGGSHELVLSYRFNQKNPNDVSFFCY"
     gene            2574..3719
                     /locus_tag="PLJ82_06870"
     CDS             2574..3719
                     /locus_tag="PLJ82_06870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR41261.1"
                     /translation="MQNFIFQNTTKLIFGKGSIASLANAIPSTARLLLVFGGGSVRTN
                     GVYDQVITSLSLHTTTEFWGIEPNPTVETIRKAVLVGKDCRADYVLAIGGGSVLDAVK
                     LIACAIPSNDDAWQLVLNGKLIKNPLPFASVMTLPATGSEMNRGSVISNKATQEKFAF
                     YAQYPQFSILDPETTFSLPKVQLANGIADTFVHVMEQYLTTPNQSLLMDRWAEGILQV
                     LVEIAPSVLANNRDYDVMSQFMLAATMALNGFISMGVSQDWATHMIGHELTALHGIDH
                     AQSLAVVLPGTMKVLQTQKAEKILQYGARVWGILQGDDNQRITKSIEATETFFRSLGL
                     ATRLSQLQIGDATIMEIEKRFMQRNVHFGEARTVTAQVTTAILNEVR"
     gene            complement(3754..4527)
                     /gene="budA"
                     /locus_tag="PLJ82_06875"
     CDS             complement(3754..4527)
                     /gene="budA"
                     /locus_tag="PLJ82_06875"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOR41262.1"
                     /translation="MKRILTGIAFVVCISSATFATTPDTDVLFQTSTINALLEGVYDG
                     EVKCGELKHYGDFGIGTFQALDGEMIFVDGTIYQVKANGKVYNVADTVKTPFASVTFF
                     DTDVSYTLQKTTNFQQLCTYLDTLIPTKNIFYAFKIEGTFAYIKTRSVPAQNKPYPVL
                     TEVTKNQPVFEFQDIQGVIIGYRIPPFADKINVPGYHFHFLSSDKTVGGHLLEMETNK
                     ANIAIDYTHRFYLSLPEESAFYTTDLSTDKTKELEKAEK"
     gene            complement(4543..6591)
                     /locus_tag="PLJ82_06880"
     CDS             complement(4543..6591)
                     /locus_tag="PLJ82_06880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOR41263.1"
                     /translation="MTSYANPFLTDRYTTPYQTVPFNEIKEEHYKEAFLKGIEEQLTE
                     IDSLLANKTTPTFENTIVALEKTGDLISRTSAVFFNLLHAETNDTLENLAQELSPILT
                     EHSNKIYQNQALFERVKAVYAQKETLTLTAEQQQLLENTYQAFVRSGAELSEKDKQTY
                     TELSTKLSQLTLTFSQYVLKETNAYSYVITDEKELDGLPDYAKEAAAEKAKAKNQEGW
                     LIDLSMPSYLPIMQYATNRELRKKLYFAYNTKGNQGNEYDNKQTITDIVNTRLQLAQL
                     MGYSNYATYKLVRTMAQDTSKVYALIDQLYTAYQPVASQEFDEIQAFARTFENNATGE
                     LMPWDWAYYAEKVKEQKYQVNDELLKPYFELEKVKQGVFGLATALFDLQFTKNTSIQV
                     YHPDVDAYEVHDSKGNFIAILYTDFHPRESKKGGAWMTEFKEQWIENGENSRPHISIV
                     MNFTPPTATSPSLLTFNELTTLLHEFGHALHGMLSNTTYASLSGTSVYRDFVELPSQL
                     LENWATEKEFLDTFAFHYQTGEPIPSQLIEKIKKAEQFLVGYACLRQLSFCYLDMAWH
                     TLSTPFSGDVKSFETDAWKKTLLFEPIAETCMSTQFNHIFSGGYAAGYYSYKWAEVLD
                     ADAYEVFKQQGIFNKKTATSFAENILSKGGSEHPMILYKRFRGQEPTIDALLKRNGIK
                     "
     gene            complement(6657..>7023)
                     /locus_tag="PLJ82_06885"
     CDS             complement(6657..>7023)
                     /locus_tag="PLJ82_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HOR41264.1"
                     /translation="IGFINDKKEIIVHKRDCKEALKLKSQKGSSIITAEWANTQKKYY
                     ATTIEVQGVDRKRILLDILTEISDKLDANIEKLCVETNDGIFSAKMNVYVSHVDTINQ
                     IYKQLKIINGVQRVARIEE"
BASE COUNT         1938 a         1252 c         1447 g         2386 t
ORIGIN      
        1 ggattgacac caggtataaa tactatgcga tggacattgc gtgggtatgg atgtacggct
       61 tacgacgaaa tgcaattacg ttatgcagaa gaacccattg cagattttga accagatgtg
      121 atttcaggat gtagtccact tacagtaggg tttgccaata caaccattgg tcaggctacc
      181 tataattggg attttggtga tggtaaaaca tctacacaac gtagtccaga gcacacattt
      241 gaacgttctg gtttgtacga agttacatta acggcagtag gcttacgcaa aacagataag
      301 ataaccaagc tgatagaagt attaccatcg cccaaggcag cgtttttagt agcaaatacg
      361 gaattattca tgccttatcc gatggctaat ttctatagca atacccctga agctataaaa
      421 tattggtggc gttttggaga tggtgccact tcaactgaaa aagatccaac gcacttgtat
      481 caattagagg gtatttacga tgtatggtta aaggtggaag atgagaaagg ttgccaagac
      541 tctattatgg tttacaatca tattcatgtg ggtgattacg gtatgatggt attccctaat
      601 gcctttacac caaatacatc tatccctaat ggaggtgtat accaagcaga agagagaagg
      661 ttggatattt tctaccctgt ttcaagaaat gttgatacct ataaattaga gatttttaac
      721 caatggggag caaaagtctt tgtatctaat gatatctttg tgggctggga cggatactat
      781 aaaggtgagt ctgcacctca gggtgtatat atgtataaag caacaggttc atataaaaat
      841 ggtagaaaat ttacggtatc tggagagttt atattggtta gatgaaaaga gtgcgaagta
      901 taattacaat gaaataccaa ctaattgcaa taatttgttt tttcgtatta tttgttagcc
      961 ctaaaaaggt tgtggcacaa gatttcttgg tgtcgcaacc ttggtcaacg aatacaactt
     1021 taggtccatc atttgcgggg ctaactggtt cgcgtgcctt tatggcatat cgcgatcaat
     1081 ggtcgtttat gagaggctcg caaaccttgt tagcaggttt tgatcactac tatcacccta
     1141 tacagtcttc ttttggagct gttgttgcac attcttctta cgggggcaat ttattgactc
     1201 aaacagaatt ggcgtttcaa tataattatg tggcaaagat tactgatgaa tggctttttc
     1261 gtccaggcat tgaatttggt ccttattata gagcaattga cccttcgaag tttatgttta
     1321 ttgatcaaat agctcttgat ggcacaatat taccaacgag ttcgttcgaa cctgatgaaa
     1381 catctgtgtt acgatttgat gcagcagtgt cctttttact gtataatgaa ttctttttta
     1441 taggtgcagg tgctgatagg ttaatgagca atgatgtctc ttttgcgaat acgaacacta
     1501 agactgactt aaaatttaat gcgtatatgg ctggtcgtat tcctatgaac agaggcatgg
     1561 gcaaatatgc atcctatgat gatgtaacga ttgctggatt attccaacaa caaggcttat
     1621 accaacaaat ggatttagat gtattgtatc atcgtattcc atttgaattg ggtatcgggt
     1681 atcggggtat acctttcgtc tcccctcaag gcttgagtaa tactgatgct ttaaagttga
     1741 tatttggata ttcctttggt gatattgtgg tatcgtatag ttatgatgta agcatatcta
     1801 gtttatttaa tgtatcaggg ggttctcacg agttggtgtt aagttaccga tttaatcaaa
     1861 aaaaccctaa cgatgtttcg ttcttctgct attgattatc tttcttgaaa tgtaaacctt
     1921 gctaggcgta gtgtctttaa ctttgttgac tttcaattcg ctatgtgtgg gttaaaaaca
     1981 cagctcccgc gtgattgctc gtcgggtgtg aaccaacggt cttgaccttg cgaaaaattg
     2041 taatcacacc cttagttcgc cgtagtgtga agtttgacaa tctgggtgtt cggcatagcg
     2101 tctcgctatg tcgaagttat accgattgca caaacaatat agcccaattt cgacaaagtc
     2161 tcattgtcct atttgtttgt cccgctatgc gggatgttcc atacgcatct cgaaatactc
     2221 aatcggcatt aacaacagta aacatataaa ataattttgg gttatgttat atgtacagtt
     2281 ggtattaaaa ggaaagctcc agctttccat attcgaacca aaccaccgct accgcacgaa
     2341 ctacgctaaa cgctatacgc tacaccagcg ctactgcaag attaatgtca ttacccgcac
     2401 gattgtattg cttaggcagg aggtaggtat atatacatag ttgatagttt ttgacgaata
     2461 acaaaaaacg ttgtttcttt tctctctata cacaaaacat tcaacgccaa ccattcacca
     2521 ctttcaactt atttttttta tctttgtaag gtatcaattc atactttata tatatgcaaa
     2581 actttatatt tcagaatacc acaaaattaa tttttggcaa aggtagtatt gcttcgttag
     2641 caaatgcaat tccttctaca gcacgtttgt tgttagtatt tggaggtggt agtgtacgta
     2701 caaatggtgt gtacgatcag gtaatcacta gtttatcact gcatactact accgaatttt
     2761 ggggtattga acccaatcca acagtagaaa ccattcgcaa ggctgtgcta gttggcaagg
     2821 attgtagagc cgactatgtg ttggcaatag gtggtggttc tgtgttagat gcggttaaat
     2881 tgattgcttg tgccattccg agcaacgatg atgcttggca gctggtgctt aacggtaagc
     2941 tgataaaaaa tcccttgccc tttgcttcgg ttatgacctt acctgctacg ggttcggaaa
     3001 tgaaccgtgg ttcagttatt tccaataagg ctacgcaaga gaaatttgcc ttttatgcac
     3061 agtatcctca attttctatt ctcgacccag aaacgacctt ttccttaccc aaggtacaat
     3121 tggctaatgg catagcggat acctttgtgc atgtgatgga gcagtatctt accaccccca
     3181 accaatcgct tttgatggat agatgggcag aggggatatt gcaggtattg gtagagatag
     3241 ctcctagtgt gcttgccaac aaccgcgatt acgatgtgat gagccagttt atgttagctg
     3301 ctactatggc tctcaatggt tttataagca tgggtgtatc gcaagactgg gctacacaca
     3361 tgatagggca cgagcttaca gctttgcacg gtatcgatca tgcacagagt ttggctgtcg
     3421 tattgccagg tacgatgaaa gtattgcaaa cgcaaaaagc tgaaaaaatt ttgcaatacg
     3481 gtgctcgggt atggggcatt ttgcaaggag atgataacca gcgaattact aaatccatag
     3541 aagctaccga aacgtttttc cgctcattgg gattagccac tcgccttagt caactccaaa
     3601 ttggtgatgc tacgataatg gaaattgaaa aacgatttat gcagcgtaat gtgcattttg
     3661 gcgaagcacg taccgttaca gctcaagtta ctacagctat tttgaatgaa gtgaggtagg
     3721 ggcagaaatt gtgataatag ctatttgatg tatttatttc tctgcttttt ccagttcttt
     3781 tgttttatct gttgataaat ctgtggtata aaaagcactt tcttcaggca gggataaata
     3841 aaatctgtgt gtataatcaa ttgctatgtt tgccttattc gtttccattt ccagtaaatg
     3901 tcctccaact gttttgtcgg atgataaaaa atgaaaatga tagccaggta cgtttatttt
     3961 atcagcaaaa ggaggtattc tgtaaccaat tattacacct tgtatgtctt gaaactcgaa
     4021 tacaggctga ttttttgtaa cctctgttaa aaccggatag ggcttatttt gagcgggaac
     4081 actccgagtt ttaatgtacg caaaagttcc ttctatttta aaagcataaa agatgttttt
     4141 ggtaggtatc agggtgtcaa gataggtaca tagttgctga aaattagtcg ttttttgtaa
     4201 ggtataactc acatctgtat caaaaaatgt aactgatgca aagggtgttt ttactgtgtc
     4261 tgctacatta tacactttgc cgtttgcttt tacctgatag attgtgccat ctacaaaaat
     4321 catttctcca tcaagagcct gaaaagtgcc tatgccaaaa tctccatagt gttttaactc
     4381 tccacacttt acttctccgt catatactcc ttctaacaag gcattgatag ttgaggtctg
     4441 aaacagtaca tctgtgtcgg gtgttgtggc aaacgtcgca ctactaatac acacaacaaa
     4501 cgctattcct gttagtattc ttttcatggt aaacctcatt ttttatttaa ttccatttcg
     4561 tttgagtagg gcatcgatag tcggttcttg acctctaaat cgtttataca aaatcatagg
     4621 atgttcggag cctcctttgc ttaaaatatt ttctgcaaat gatgtagctg tttttttgtt
     4681 aaaaattcct tgttgtttaa atacctcata tgcatcagca tccaatactt ctgcccattt
     4741 gtagctgtaa tatcctgctg cgtatcctcc cgaaaatata tggttaaatt gtgtgctcat
     4801 acacgtttcg gcaatgggtt caaaaagcaa tgtttttttc caagcatctg tttcaaagct
     4861 ttttacatcg ccactaaaag gtgttgaaag cgtatgccat gccatgtcta agtagcaaaa
     4921 acttagttga cgcaaacaag cataccctac caaaaattgt tccgcttttt ttatcttttc
     4981 aatcaattga gatggaattg gttcgcctgt ttgataatga aaagcaaagg tatcaagaaa
     5041 ttctttttct gtagcccaat tttcgagcaa ttgagaggga agttctacaa agtcgcggta
     5101 aacgcttgtt ccagagaggc ttgcgtaggt ggtgttggat aacataccgt gcaaagcgtg
     5161 tccaaattcg tgtagcaagg tcgttaattc attaaaagtg agcaacgacg ggctagtagc
     5221 tgtgggtggt gtaaagttca ttacaatgga tatgtggggg cgactatttt ctccgttttc
     5281 aatccattgt tctttaaact cagtcatcca tgcacctcct tttttgcttt ctcttggatg
     5341 aaaatcggtg tataatatgg caataaaatt ccccttgcta tcatgcactt cataggcatc
     5401 tacatctgga tgatacactt gtatagaagt gttttttgta aactgtaaat caaaaagggc
     5461 agtagctaga ccaaacactc cttgttttac tttttcgagt tcaaaatagg gtttgagtaa
     5521 ttcgtcgttt acttggtatt tttgttcttt cactttttcg gcataatatg cccaatccca
     5581 aggcatgagt tcacccgtag cattgttttc aaacgtgcga gcaaaggctt gtatttcatc
     5641 aaattcttgc gaggcaactg gctgataagc agtatatagt tggtcgataa gtgcatatac
     5701 cttgctggtg tcttgagcca tggtacgtac tagcttgtag gtagcgtagt tgctgtaacc
     5761 cattagttga gcaagttgca gacgggtgtt aacaatatct gttatggtct gcttgttgtc
     5821 gtattcattt ccttgattcc ctttggtgtt gtaagcaaaa tatagttttt tgcgtaactc
     5881 gcggttggtt gcatattgca ttatgggcaa ataacttggc atcgaaaggt cgataagcca
     5941 accttcttga tttttggctt tggctttttc tgcagctgct tctttggcat aatcaggcaa
     6001 tccgtccagt tctttttcgt cggtgattac gtatgaataa gcgtttgttt ctttcagtac
     6061 atattggcta aacgtaagtg ttagttggct tagcttggtg cttaattcag tgtacgtttg
     6121 tttgtctttt tctgataatt cagcccctga gcgtacaaat gcttggtagg tattttcgag
     6181 taattgttgt tgttcggctg tcagagttaa ggtctctttt tgagcatata cggcttttac
     6241 tcgttcaaaa agggcttgat tttggtaaat cttattgctg tgttcagtta gtatcggaga
     6301 tagttcttga gccagatttt ctaaggtatc gtttgtttca gcatgcaata agttgaaaaa
     6361 tacagcactt gtgcggctta ttaaatctcc tgttttttct aatgctacta tggtattttc
     6421 aaatgttgga gtcgttttat tggctaagag agagtctatt tcagttagtt gctcttctat
     6481 tcctttcaga aaagcttctt tgtaatgttc ttcttttatc tcgttaaaag gtacagtttg
     6541 gtaaggggtt gtataacgat cggttaaaaa agggtttgcg taactagtca tagaaaaaag
     6601 tattagataa aataaaatgt gagatatata ggcattcata tgtctgtata aaggtgttat
     6661 tcttcgatac gtgctacacg ttgtacacca ttaataattt tcagttgctt gtatatttgg
     6721 ttaattgtat ctacgtggct tacatacacg ttcattttgg cagaaaaaat accatcgttt
     6781 gtttctacgc ataatttttc aatattagca tctagtttat cagatatttc agttaaaata
     6841 tccaacaaaa tacgttttct atctactcct tgtacttcga tagtggtagc ataatatttt
     6901 ttttgtgtat tagcccattc ggcagtaatg atgctcgacc ctttttgcga tttgagtttt
     6961 aacgcttctt tgcagtctcg tttgtgtacg attatttctt ttttgtcatt tataaaacca
     7021 ata
//
LOCUS       DAOSRP010000102         6872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_10228_length_6872_cov_5.082148, whole genome shotgun sequence.
ACCESSION   DAOSRP010000102 DAOSRP010000000
VERSION     DAOSRP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10228_length_6872_cov_5.082148"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLJ82_06890"
     CDS             <1..271
                     /locus_tag="PLJ82_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR41265.1"
                     /translation="PMNVSYLDTYAYILFKQKRYETALFFIEKAMEYTKDGEKNAVVY
                     ERYGDILYFLGQQTKALEMWQKALELTQDATDTIKQKVNTQKYVE"
     gene            261..1061
                     /locus_tag="PLJ82_06895"
     CDS             261..1061
                     /locus_tag="PLJ82_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOR41266.1"
                     /translation="MSNKSGIRLLLACMLLILVACKTTKIDTAQSVVPIQVPLKKIVA
                     NQQQIQTINASKVSFSIPMQNQQTPLRGSIKARIDSCMQISIQALFGIEVARIHLTQD
                     SLILLDRINQQYVAESFSSFGSTFPVSFRMLQAIILNRLVDVTTKDDFSDFLYQKDEN
                     LHIVSYENSNFAIQYLVNDALHLLRTTVRDAEKRHYMMAEYQQFDTYNTIVYPKKNAI
                     VMLFSQQQYAIDYSIEKVEFNQQIDLSIVIPPRYKQILLADIAKEYFN"
     gene            1067..2269
                     /locus_tag="PLJ82_06900"
     CDS             1067..2269
                     /locus_tag="PLJ82_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOR41267.1"
                     /translation="MKKILSILFIFASVVLPALSQTVAELEERRKKALKELEITSSLI
                     SKTAEQKKTSLSQINILRVEINARQSLINVMNQELRNIDRNIGQLRKEAQEKQAEMDK
                     LKGEYAKLMYHSYYKKTKYESLMFILSAKSFAESYRRYRYVQQYAEFAQNKAQEIEKV
                     KADLENKLAEIEKARLERVAVLNDRRKENAKLQTEQRKQSNFVSQLSRKEKDLRRELK
                     KQQDQANAINRRIDQIIAEEARKSQKAQAAGTQEFALTKEQKLLSGGFEKNQGRLPWP
                     VERGVIVGRFGVQAHAVLKYVTTDNKGIYIQTAPNSDARAVYDGEVTQRFSIPGSNYT
                     VIVRHGNYRTVYSNLTDIYVKVGDNIKARQAIGRIFVDNEDDNKSVLYFQVWKERDIQ
                     NPENWITR"
     gene            2469..3770
                     /locus_tag="PLJ82_06905"
     CDS             2469..3770
                     /locus_tag="PLJ82_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HOR41268.1"
                     /translation="MPKTFFSKYKIPTKLQIPVTSFLFKWLFFALLVGVLAGSAAALL
                     LVSLDFVTNIRESNVWLIGFLPLAGLGIGLMYHYLGSSVVKGNNQILEQIQSPTQTIP
                     FRMAPLIFIGTVLTHLFGGSAGREGTAVQMGAAISDQFAKWFKLSSNDKRILLIIGIS
                     AGFAGVFGTPLAGAIFALEVVVIGRMRYEAILPAFLAAAIAHFTCHAWGVAHTVYSIP
                     FVPELTPVSLVSAIAAGIIFGMVAMLFSKTGHWWTGLFSRFIPYPPLRPFAGGMIIAM
                     AVWMLGTTKYIGLGVPTIQASFDTEMQGYDFLLKLLFTTFTIGAGFKGGEVTPLFFIG
                     ATLGNVLVWFFPLPMPLLAGMGFVAVFAGATNTPLACTMMGIELFGAESGLYIGIACV
                     VAYFFSGHTGIYAAQKVGSPKHVAFLKQQGESEAETRSVRS"
     gene            complement(3773..4306)
                     /locus_tag="PLJ82_06910"
     CDS             complement(3773..4306)
                     /locus_tag="PLJ82_06910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOR41269.1"
                     /translation="MNILAFGASHSKSSINQQFASYVAQLFSAGNQVSVIKLTDFDAP
                     VYTVDREAESGFPEAVKQFLLQLQKADLIIISLAEHNGSYTASFKNLFDWTSRVEMNL
                     YANKKLFLLSTSPGPNGGKFVMEAAKVRFPIHGAAIVGSFSLPRFADNFDAQKGITNK
                     ELKEAFDTEIEKVTTAL"
     gene            complement(4395..6773)
                     /locus_tag="PLJ82_06915"
     CDS             complement(4395..6773)
                     /locus_tag="PLJ82_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41270.1"
                     /translation="MKKLFLLISTFFLIQTVLFSETGQTVRGVITDAFSGQPLIGASI
                     VVADSNPLQGTIADENGKFELNNVPLGRSTFIVSALGYHATSMSNIMVISGKETLLQI
                     ALEEKITSLKDVVVTANFSKNKPINDLALVSSRSFSVEETERYAGSLGDPARMVANYA
                     GVVVGNDSRNDIIIRGNSPSGLLWRMEGVAISNPNHFGAHGTTGGPVTMLNSNLLANS
                     DFMTGAFSAEYGNALSGVFDLNLRTGNTERFEGMGQVGFNGFELLLEGPIKIGNFIKN
                     GSFIADYRFSTLQLMSLLGFDIGTGGTVPEYNDLSVMIDLPTSKIGRFKLIGLLGNSF
                     ISMGRSFDIDEAITHNQMGYATDFGAGLNIAMLTHTYMFNEHSRLKSSLSFEHATSTT
                     LSDSINYTDKKYFNLYAANYKENKWMANTQFKRRINAKNNLTLGASASLFVSSFFDSV
                     FVNSLDKRITLTAIDKEPSILYNAYTNWQHKFTNELSLNTGLHYQLYNLTNESAIEPR
                     LALRWQAHQQHAFSLGYGLHSQIQARTIYFYEAYYKDTDTYARNNMTTGYTRSHHIVG
                     GYDWTISSYWRVKTEAYYQDLFNVPVSPISGSYSALNSGSGYYIDNIDSLQNTGVGRN
                     YGLEMTIEKFLHKGYYGLMTFSLYDSKYQGYDKIWRNTAFNSNFAVNLLGGYEWRVGT
                     KNFLAVDVRTIWAGGMRYVPIDLAASIAAGEQRLASTDIYTQRFNDYFRCDVRISFKQ
                     NLGKVSQEWGLDLQNVSNYKNIFAQQYNKYSESIATVYQQGFMPMMMYRIHF"
BASE COUNT         2140 a         1295 c         1363 g         2074 t
ORIGIN      
        1 gcctatgaat gtgtcctatt tagatacgta tgcatacatt ttatttaagc agaaacgcta
       61 cgaaacggct ctatttttta tcgaaaaagc catggaatat accaaagatg gagagaagaa
      121 tgcagtggtt tacgaacgct atggcgatat cttatatttt ttaggacaac aaaccaaagc
      181 actcgaaatg tggcaaaaag cccttgaatt aacccaagat gctacggata caataaaaca
      241 aaaagttaac acccaaaagt atgtcgaata aatcaggaat cagattgttg ttagcatgta
      301 tgttgctcat tttggtggcg tgtaaaacaa caaaaataga cactgctcag tctgtggtgc
      361 ctatacaagt accgttgaag aaaattgtgg caaaccaaca gcaaatacag acgataaatg
      421 cttcgaaagt aagcttttct attccaatgc aaaaccaaca aaccccacta cgaggctcta
      481 tcaaagcaag gattgatagt tgtatgcaaa tttccataca agccttgttt ggaatagaag
      541 ttgctcgcat acaccttacg caagatagtc ttattttact tgacagaata aatcagcaat
      601 atgttgctga atctttttcg tcgtttgggt caacgtttcc agtttctttt cgtatgttgc
      661 aagcaattat ccttaataga ttagtagatg taacgacaaa agatgatttt tcagattttt
      721 tataccaaaa agacgaaaat ctacacatag tatcgtacga aaatagtaat tttgcgattc
      781 aataccttgt aaatgatgct cttcatttgc tacgaacaac agtaagggat gcggaaaaaa
      841 ggcactatat gatggcagaa taccagcagt ttgacacata caatacgata gtatatccaa
      901 agaaaaacgc tattgtgatg ctcttttctc agcaacaata tgctatagat tatagtatcg
      961 aaaaagttga gtttaatcaa caaatagatt tatcaattgt tattcctcct agatacaaac
     1021 aaattttgct ggctgatatt gccaaagaat attttaacta ataattatga aaaaaatact
     1081 ttctattcta tttatttttg ccagtgtggt attacctgct ctttctcaaa ctgtagcaga
     1141 gcttgaagag cgtcgtaaaa aagcccttaa agaattggaa attactagtt cgttgattag
     1201 taaaactgct gaacaaaaaa agacctcttt gtctcaaatt aatattttgc gtgtagaaat
     1261 taatgcccgt cagagtttaa ttaatgtgat gaaccaagaa cttcgtaata ttgatagaaa
     1321 tattggtcag ttacgcaaag aggcacaaga aaaacaagca gaaatggaca agttaaaagg
     1381 cgagtatgct aaattaatgt accattcgta ttacaaaaaa accaaatatg aatccctgat
     1441 gtttattttg tcagctaaaa gttttgccga atcgtatcgc agataccgct atgtacaaca
     1501 gtatgctgaa tttgctcaaa ataaagccca agaaatagag aaagtaaaag cagatttaga
     1561 aaacaaatta gcagaaatag aaaaagcccg tttagagcgt gtggctgtat taaatgatag
     1621 acgaaaggag aatgctaaat tgcaaaccga acaacgtaag caaagcaatt ttgtttcgca
     1681 attaagtaga aaagaaaaag atttgcgtag agaacttaaa aagcaacaag atcaagccaa
     1741 tgccattaat cgtcgcatag atcagattat tgcagaagaa gcccgcaaat cacaaaaagc
     1801 acaagccgca ggcacacaag aatttgcttt aacgaaagaa cagaaactgc tttctggagg
     1861 ctttgaaaag aaccaaggtc gcctaccatg gcccgttgaa cgaggcgtaa ttgtagggcg
     1921 ttttggcgtg caagctcatg ctgtgctaaa gtatgtaaca actgataata aaggtattta
     1981 tattcaaact gctccaaatt cggatgcacg tgctgtatat gacggggaag taacccaacg
     2041 tttctccatt ccaggaagca attatacggt gattgtacgc cacggtaact accgtacggt
     2101 atattctaac ttaactgata tatacgtaaa ggtaggcgat aatatcaagg ctcgtcaagc
     2161 cattgggcgt atatttgtcg acaacgaaga cgacaataaa tccgtactct attttcaagt
     2221 atggaaagaa cgcgatatcc aaaaccccga aaactggatt actaggtaaa aaaggggatt
     2281 gtttgagggt ggttaatgct ttatatcgaa gttgattata aagacaatta cctgatatag
     2341 gttgcagact tgctacagcg atgaggattg aatcaaatag ataatccgtt ggataaatta
     2401 tatttaggat aagacaaata ccttactcaa tatgattact tttgcaacat agttttacaa
     2461 cagattgtat gcccaaaaca tttttttcga aatataaaat cccaacaaaa ctgcagattc
     2521 ctgtaacaag ctttttgttt aagtggttat tttttgcttt gttggtggga gttttggcgg
     2581 gttcggcggc tgctttgttg ttggtatcgt tagattttgt aaccaatatt agagaaagta
     2641 atgtttggct aatagggttt ttgcccttgg cgggtttggg tatcggtctg atgtatcatt
     2701 atttaggttc gtcggtagta aaaggcaata accaaatact ggagcaaata caatcaccta
     2761 cgcaaactat ccctttccgt atggcaccac tcatttttat tggtacggtg cttacgcatt
     2821 tgttcggtgg ttctgctggt cgagaaggta cagccgtaca aatgggagca gccatttccg
     2881 atcaatttgc taaatggttt aagctatctt ctaacgataa acgcattttg cttattattg
     2941 gtattagtgc tggttttgca ggggtgtttg gtactccctt ggctggtgct atttttgctt
     3001 tggaagttgt ggtgataggt cgtatgcgtt acgaagctat tttgcctgct tttttggcgg
     3061 ctgctattgc tcattttacc tgtcatgctt ggggtgtagc tcatacggtt tactccatac
     3121 cttttgtacc cgaacttacg cctgtcagct tggtatcggc aatagcggcg ggaataatct
     3181 ttgggatggt ggctatgcta ttttccaaaa ctggtcattg gtggacgggt ttgttctcgc
     3241 gttttattcc ttatccacct ttgcgtccgt ttgctggcgg tatgattatc gctatggcag
     3301 tttggatgtt ggggactacc aaatacattg gattgggggt tcctaccata caagcttcat
     3361 tcgacaccga gatgcaagga tatgattttc tactaaaatt gttatttacc acatttacca
     3421 ttggagctgg tttcaaagga ggagaagtaa ctcctttgtt ctttattggt gctacattgg
     3481 gtaatgtgtt ggtgtggttt tttcctttac ccatgccatt gcttgcagga atgggatttg
     3541 tggcggtttt tgcaggagcg actaatacgc cgcttgcctg caccatgatg ggaatagagt
     3601 tatttggagc agaaagcggt ttgtatatcg gtattgcttg cgtggtggct tatttctttt
     3661 cgggtcatac aggtatttat gctgcccaaa aagtgggaag cccaaagcat gtagcatttc
     3721 tcaagcaaca aggagaaagt gaggctgaaa cccgttcggt gcgatcgtag gtttacaaag
     3781 ctgttgttac tttttctatt tctgtgtcaa acgcttcttt tagttccttg ttcgtaatgc
     3841 ctttttgtgc atcaaaatta tccgcaaaac gaggcaatga aaagctacca acaattgctg
     3901 ccccatgaat gggaaagcgt actttggcag cttccattac aaattttccg ccatttggac
     3961 caggagaagt agaaagtaag aataattttt tgttggcata aagattcatt tctacacgag
     4021 aagtccaatc gaacaagttt ttgaacgatg cagtataact accattgtgt tcggctaagg
     4081 aaataataat caaatctgcc ttttgtaact gaagtaaaaa ctgttttact gcttcgggaa
     4141 aaccactttc tgcctcccta tctaccgtat atacaggagc atcaaagtca gtaagcttta
     4201 taacagacac ttggttccct gccgaaaata actgtgcaac atagcttgca aattgttggt
     4261 tgatggagct tttgctatgg ctagctccaa atgctagtat attcatggtg tgctattttt
     4321 tagtttatga agcaaaaata gcaatttaac aatgcgtttc caattctttt attaacattt
     4381 ctgcttcctt aacattaaaa atgaattcga tacatcatca taggcataaa gccttgttgg
     4441 taaacggtag ctatactttc tgaatatttg ttgtattgtt gtgcaaaaat gtttttataa
     4501 ttgctcacgt tttgcaaatc caatccccat tcttgcgata ctttgcctaa gttttgttta
     4561 aaactaattc gcacatcgca cctaaaataa tcgttgaacc gttgcgtata aatatcagta
     4621 gaagctaaac gttgttcgcc cgctgcaata gaggcagcca aatcgatagg cacataccgc
     4681 ataccgcctg cccaaatggt gcgaacgtca acagccaaaa agttttttgt accaacacgc
     4741 cattcgtaac cacccaataa gttgacagca aaattggagt taaaagctgt atttctccat
     4801 attttatcat acccttggta ttttgagtca taaagcgaga aggtcattaa accataatac
     4861 cccttgtgaa ggaatttttc tatggtcatt tccaatccat aatttcgtcc gacacccgta
     4921 ttttgcagac tatcaatatt gtcaatgtag tagccactac ccgaatttaa ggcagaataa
     4981 ctgccactaa tgggcgaaac aggcacattg aataaatctt ggtaatacgc ctccgttttt
     5041 acacgccaat acgaagaaat tgtccaatcg taaccgccca caatgtgatg cgatcgcgtg
     5101 taaccagtag tcatattatt gcgagcatac gtatcggtat ctttgtaata cgcctcataa
     5161 aaatatatgg tacgagcttg tatctgactg tgcaatccgt aacccaaaga gaaagcgtgt
     5221 tgctgatgag cttgccaacg tagagcaagg cgaggttcaa tggcactttc gtttgttaaa
     5281 ttatacaatt gataatgcaa gcctgtattg agcgacaatt cgttggtaaa cttatgttgc
     5341 caattggtat acgcattgta caagatgctt ggttctttgt caatagctgt tagggtaatg
     5401 cgtttatcca aactgttaac aaataccgaa tcaaaaaaac ttgatacaaa aagactagca
     5461 gaagcaccca aggtaaggtt gttttttgcg tttatcctgc gtttaaattg tgtgtttgcc
     5521 atccacttat tttctttgta attggcggca tataggttga aatatttttt gtcggtatag
     5581 tttatcgagt cagacagcgt ggtagaagta gcgtgctcga aagaaagact gctttttaaa
     5641 cgggaatgtt cgttgaacat atacgtgtgg gttaacatcg caatgttcaa tcctgcacca
     5701 aaatcggtag catatcccat ttgattgtgg gtaatggctt catctatatc aaaagagcgt
     5761 cccatcgaaa taaaactatt gcctagtaaa ccaatcagct taaagcgtcc aattttgctg
     5821 gttggcaaat caatcatcac cgataagtcg ttgtattcgg gcacagtacc tcctgtgcct
     5881 atatcaaaac ccaacaggct catcagttgc aaggtagaaa aacgataatc ggcaataaac
     5941 gaaccatttt taataaaatt accaattttt atgggaccct cgagtagcaa ctcaaaacca
     6001 ttaaagccta cttgtcccat gccctcaaaa cgttcggtat tacctgtgcg taggtttaaa
     6061 tcgaatacac ccgacaaggc attgccatat tcagcactaa acgccccagt cataaaatca
     6121 gaatttgcca ataagttgct attgagcatg gtaacaggtc cgccagtagt gccgtgtgcc
     6181 ccaaagtggt tggggttgga tatggctaca ccttccatac gccacaacaa acccgaaggc
     6241 gaattgcccc tgatgataat atcattgcgc gaatcgttgc caactaccac acccgcataa
     6301 ttggctacca tacgggcagg atcgcccaaa ctgccagcat aacgttctgt ttcttctaca
     6361 ctaaacgaac gtgaactcac caaagccaag tcgttaatag gtttgttttt agaaaaattg
     6421 gctgtaacca ctacatcttt gagcgaggta attttctctt ctaaggctat ttgtaggagg
     6481 gtttcttttc ccgatattac catgatattg ctcatactag tagcgtgata gcccaaggca
     6541 ctaacgataa aagtagaacg acccaaaggc acattgttca actcaaattt tccattttca
     6601 tcggcaatgg taccttgcaa tggattagaa tctgccacaa caatacttgc accaatcagt
     6661 ggttgaccag aaaaagcatc tgttatcacg cctcttacgg tttgacccgt ttcagagaat
     6721 aatactgttt gaattaaaaa aaacgtagaa ataagtaaga acaacttttt catgctaatt
     6781 gctttaattt tggcacaaaa ataatgaata aatgctattt taacgtattt ttaccattgc
     6841 tatgcatcta atttaagaaa aatttacatt aa
//
LOCUS       DAOSRP010000103         6349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_11281_length_6349_cov_4.880203, whole genome shotgun sequence.
ACCESSION   DAOSRP010000103 DAOSRP010000000
VERSION     DAOSRP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11281_length_6349_cov_4.880203"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="rodA"
                     /locus_tag="PLJ82_06920"
     CDS             <1..1024
                     /gene="rodA"
                     /locus_tag="PLJ82_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOR41271.1"
                     /translation="ILTPMLIIILQQETGSALVFTAFFLVLFREGMNGIFMVLAFLGA
                     LFFVLFLRYDYISSSSNSYILESFGFVIVFILSVISVGIMIAYYLKDNRQMLQFIGGN
                     AILYLLTGILHLFGIEAITYTITASISISLSILYLFIMALLQKQKKYFLFIIFLIGSI
                     ALCNVTDYFFNNVLQPHQQMRIKVVLGLEDDPLGAGYNVRQSKIGIGSGGLLGKGFLN
                     GTQTKLNYVPEQDTDFIFCTIGEEQGFVGSIVVLFLYIALIIRLVILAERQRSSFSRI
                     FGYSVLSIIFFHVAINIGMVIGLVPVIGIPLVFLSYGGSSLWSFTILLFTFIRLDANR
                     MEILHQ"
     gene            1202..1447
                     /locus_tag="PLJ82_06925"
     CDS             1202..1447
                     /locus_tag="PLJ82_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOR41272.1"
                     /translation="MNIYIGNLNYRVRENDLKGIFEEYGTLDSVKLVKDRETGKSKGF
                     AFAEMSNDSEAKKAIEELNGAEFEGRTLVVKEANPRN"
     gene            complement(1506..2477)
                     /locus_tag="PLJ82_06930"
     CDS             complement(1506..2477)
                     /locus_tag="PLJ82_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOR41273.1"
                     /translation="MNKIDEIKQPIIADLLAYEKELALALQTENPILNQLSSYVLSTR
                     GKQMRPILSLLVAQVCGGITESTIQGAIAIELLHIASLIHDDVLDSALERRNKPSVNA
                     VFENKIAVLGGDYLLSKALSKASNSRNLDIIFEISKVGSNLADGEILQLANSKKQFIT
                     EDIYFDIISKKTASLFSSCARIGALSANATNHEIDCLGIYGTSIGICFQLRDDIFDYF
                     SDDVGKPTGNDLREGKLTLPVLFALSKATNSEKEYLFSLLRKADLTEKEVQELQQFSI
                     QKGGVEYATKKMYEYRDKAIRQLQHFATSATKNALMEYATYVIERNK"
     gene            complement(2482..3438)
                     /locus_tag="PLJ82_06935"
     CDS             complement(2482..3438)
                     /locus_tag="PLJ82_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOR41274.1"
                     /translation="MMYAERSKEVFRQEIQALEKVASRIGAEMDKVVELLFASTGKIV
                     VTGIGKTGIIAHKIASSLASTGTPAFFMNAAEAMHGDLGMISANDVVLIVSNSGASQE
                     ILRIIPPIKHINCPIIALTGNVSSPLANEADYVLDVSVDKEACPLGLAPTTSTTATLV
                     MGDALIVSLMEKRHFKADDFALNHPGGALGKRLLVKVKEIMQTTIPYVYEKTPFKDVI
                     YEISNKRLGMTIVMNTNNTPIGIITDGDVRRAIQKYQDISNLTAYDFMTVGYKSITEN
                     ERIDDALRIMETNKITTLAVVDAATPTIIGIIHIHDIFAYRK"
     gene            complement(3458..4294)
                     /gene="kdsA"
                     /locus_tag="PLJ82_06940"
     CDS             complement(3458..4294)
                     /gene="kdsA"
                     /locus_tag="PLJ82_06940"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOR41275.1"
                     /translation="MIIPSTQQVVIPNHFVIGGTSRFVFIGGPCAIESEQMSIDVAGS
                     LKEICDKLGIHFVFKSSFDKANRSSVFSPRGVGMDKGLSILQKVTDTYGIPILTDVHE
                     SWQCDAVGSVVDVIQIPAFLARQTDLLVAAAKTGKVVNIKKAQFMAPWDMQQVVNKMI
                     ETGNDSIVLTERGSSFGYNNLVVDMTSLIEMRKIGYPIVFDATHAVQKPGGNGINSGG
                     NRSYVAPLMRAALAVGVDAIFAEVHPNPDMAFSDAANQLHLDSIHTILEQAVTIDDII
                     KK"
     gene            complement(4291..4836)
                     /locus_tag="PLJ82_06945"
     CDS             complement(4291..4836)
                     /locus_tag="PLJ82_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOR41276.1"
                     /translation="MNVCVFCSSSNEIAEVYREDARLLGEVIGKKSYTLVYGGANMGL
                     MQECAMAVKLSGGSVIGVIPDSFRKKVEQKVLDQVFFVGSLAERKELMMEYADVFVAL
                     PGGIGTLDEVFEVLALAMIDNHHKKVLFLNTHGYYTHLVAHIEHSIQEKCVHPAMKNS
                     YIVVNTVDECMAYIDKENVVK"
     gene            complement(4851..>6349)
                     /locus_tag="PLJ82_06950"
     CDS             complement(4851..>6349)
                     /locus_tag="PLJ82_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOR41277.1"
                     /translation="QFSVREIGVSTQKGTTVTFKPDGSIFMTTEYKYDILAGRLRELA
                     FLNAGIRLILTDKRERFEDGSYKSEEFYSTEGLAEFVRFIDQAREKLIDEVIHISTDK
                     YATPVEIAMLYNTSYNENIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEENKLLEK
                     LEKNKIEIDGNDFREGLTAVISIKVAEPQFEGQTKTKLGNNEVMGSVDMAVSEALGNY
                     LEEHPKEARTIVEKVILAATARHAARKARDLVQRKSPLSGAGLPGKLADCAEKDPAKC
                     ELFLVEGDSAGGTAKQGRDRATQAILPLRGKILNVEKAMPHKVFESEEIRNIYTALGV
                     TIGTEEDSKALNLSKLRYHKVVIMTDADVDGSHIATLILTFFFRHMKDLIENGYVYIA
                     TPPLYLCRKGKIEEYCWDEKQRLAFVEKYGKGSEGGIHIQRYKGLGEMNAQQLKQTTM
                     DPENRTLRQVTIENAAEADHIFSMLMGDDVAPRRDFIEKNATYARIDA"
BASE COUNT         1898 a         1264 c          984 g         2203 t
ORIGIN      
        1 tattttaact cctatgttaa tcatcatact ccaacaagaa actggatctg cattggtttt
       61 tacagcattc tttcttgtat tgtttaggga aggcatgaat ggaattttta tggtactagc
      121 cttcttagga gctttatttt ttgtcctttt tttacgatat gattatattt cgtcgagtag
      181 taactcctat atactcgaat cgtttggttt tgtcatcgta ttcattttgt ctgttatttc
      241 ggtgggcata atgattgctt attacctaaa agataatagg caaatgttac aatttattgg
      301 tggtaatgct atattatatt tacttacagg aatcttacat ctatttggaa tcgaagctat
      361 tacatatact ataactgctt ctataagtat atctttaagt attctctatt tattcataat
      421 ggctttatta caaaagcaaa agaagtattt cctttttatc atatttctga ttggaagtat
      481 agccctgtgc aatgttactg attatttttt caacaatgtg ttgcaacctc atcagcaaat
      541 gcgtattaaa gtagtattag gattagaaga cgacccattg ggtgctggat acaatgttcg
      601 tcaatcaaaa attggcattg gttcaggtgg gttgcttgga aaaggatttc taaatggaac
      661 acaaacaaaa ttaaattatg ttcccgaaca agatacagat ttcatttttt gcacaattgg
      721 ggaagaacaa ggatttgtgg gaagtatagt ggtcttattt ctatatattg cattaatcat
      781 acgacttgta attttggctg aacgacagcg atcttccttt agtcgtattt ttggctattc
      841 tgtgttaagt attattttct ttcatgttgc tataaatatc ggaatggtta ttggtttagt
      901 tcccgttatt ggtattccgt tagttttttt aagctatgga ggttcttctt tgtggagctt
      961 cacaatatta ttgttcacat ttatacgtct tgatgctaat agaatggaaa tattacatca
     1021 ataactcctg tgaggtatat aaaattataa ccgaaacgta acctaaataa tgaaatatac
     1081 aacaaaatcc acattttttt tcaaattatg gtattttatt aaaatattat gctttccttt
     1141 gtgtcgtaaa tcattaagtg aagatttacg acatatttat ttattttttt aatttttttt
     1201 tatgaacatt tacattggaa acttgaatta tcgagttaga gaaaacgacc taaaaggtat
     1261 ctttgaagaa tacggaacat tggattctgt aaaacttgta aaagacagag aaacaggaaa
     1321 atcgaaaggt tttgcatttg cagaaatgtc aaacgacagc gaagcaaaaa aagctatcga
     1381 agaactgaat ggtgctgaat ttgaaggaag aacattagtg gttaaagaag caaatcctag
     1441 aaattaattg atattatttt tatgggaata acaaaagagg ctattcaatt gaatagcctc
     1501 ttttgttatt tgtttcgttc tataacatag gttgcatatt ccatcaaggc atttttagtt
     1561 gcagaagtag caaaatgttg taattgacga attgctttat ctctgtattc atacattttc
     1621 ttggttgcat attccactcc ccctttttga atggaaaatt gttgcaattc ttgcacttct
     1681 ttttcagtca aatctgcttt ccgaagcaaa gaaaatagat attctttctc gctattcgtt
     1741 gctttactca gagcaaacag cacaggtaaa gtaagctttc cttctcgaag atcatttcct
     1801 gtaggcttgc ctacatcatc cgaaaaataa tcaaatatat cgtctcttaa ttgaaaacat
     1861 atcccaatgc tcgtaccata aatacctaaa caatcaattt cgtgattggt agcatttgct
     1921 gatagtgctc ctatgcgggc acaactagaa aaaagactag ctgtcttttt tgaaataata
     1981 tcaaaataga tatcctcggt tataaattgc tttttagaat ttgccagctg caaaatctct
     2041 ccatccgcca gatttgatcc tactttagat atttcaaaga taatgtctaa gtttctactg
     2101 tttgaagcct tggataaggc tttagataac aaataatctc cacccaatac ggcaatcttg
     2161 ttttcaaaca ccgcgtttac tgatggttta tttcttcgct ctaaggcact atcaagtaca
     2221 tcatcatgaa ttaaactagc tatatgtagc aactcaatag caatagcacc ttgtatagtt
     2281 gattcagtaa tgccgccaca gacttgagca actaacaacg acaaaatagg tcgcatttgt
     2341 ttacccctcg tgcttaatac gtagcttgac aattgattga gaattggatt ttctgtttga
     2401 agagctaagg ctagttcttt ttcatatgcc aacaaatctg caattattgg ttgtttaatt
     2461 tcgtctattt tgttcataaa cttattttct gtatgcgaaa atatcatgta tatgtataat
     2521 gccaataatg gttggcgtag cagcatctac tacagccaat gtggttattt tatttgtctc
     2581 cattattctt agtgcatcat caatacgttc attttctgta attgatttat agccaacagt
     2641 cataaaatca tatgctgtta aattagagat atcctgatat ttctgaatag cccttctcac
     2701 atctccatct gtaataatgc caataggtgt attgttcgta ttcatcacaa ttgtcatacc
     2761 caatcgttta ttactaatct catatataac atctttgaaa ggtgtttttt cgtacacata
     2821 gggtatggtg gtttgcataa tctcttttac ttttactagt aatcgtttcc ccaatgcacc
     2881 tccaggatga ttaagtgcaa aatcatcggc tttaaagtgt cgtttttcca tcaaactaac
     2941 aattaaagca tctcccatta ctaatgtggc tgtggtagaa gtagttggag ctaaccccaa
     3001 cggacaagct tccttatcaa cactcacatc gagaacataa tcagcctcgt tagcaagtgg
     3061 agaagaaaca ttacctgtta atgctataat cggacaattg atatgcttaa taggaggtat
     3121 gattctcaat atttcttgcg aagctccact gttagataca atcaacacta catcattggc
     3181 ggatatcata cctaaatctc catgcattgc ttctgccgca ttcataaaaa aagcaggagt
     3241 tcctgtagaa gccaacgaag aggcaatttt atgtgcaata atacccgttt taccaatacc
     3301 cgttaccaca atctttcctg tactagcaaa caataactca actactttat ccatttctgc
     3361 acctatgcgt gatgctacct tttctaaagc ttgtatctcc tgcctaaaaa cttctttgct
     3421 acgttcagcg tacatcatac gatcggttat tagtctacta tttttttata atatcatcta
     3481 ttgttaccgc ctgctcaagt attgtatgga tactatccaa gtgcaattgg ttggccgcat
     3541 ccgaaaaagc catatctgga ttaggatgta cttcagcaaa aattgcatca acacctactg
     3601 ccaatgctgc tcgcattaat ggagcaacat aggagcgatt gcctcctgaa tttattccat
     3661 ttccaccagg tttttgcaca gcgtgcgtag catcaaaaac gataggataa cctattttac
     3721 gcatttctat taatgatgtc atatctacta ccaaattatt atacccaaaa ctggatcctc
     3781 tttctgttag tacaatagaa tcatttcccg tttctatcat tttatttacc acttgttgca
     3841 tatcccacgg agccataaat tgggcttttt ttatattcac cactttgccc gttttagctg
     3901 ctgcaactaa taaatcggtt tgcctagcta aaaaagcagg tatttgtata acatctacta
     3961 cggaacccac tgcgtcacat tgccaacttt cgtgtacatc tgtcagtata ggtataccat
     4021 atgtatctgt aaccttttgt aaaattgata accctttgtc cattcctaca ccacggggag
     4081 aaaatacaga agagcgattt gctttatcaa aagatgattt aaaaacaaaa tgtataccta
     4141 atttatcaca aatctctttc aaactgcctg ccacatctat tgacatttgt tcgctttcta
     4201 tggcacaggg tccaccaata aaaacaaaac gactagttcc tccaataaca aaatgattgg
     4261 ggattactac ttgctgtgta cttggaataa tcattttaca acattttctt tatcaatgta
     4321 tgccatacat tcgtctactg tattaactac aatatacgag tttttcatcg caggatgcac
     4381 acatttctct tgtatactgt gttctatatg agcaactaaa tgtgtatagt agccatgggt
     4441 atttaaaaac aatactttct tgtgatggtt atctatcatt gctaaggcaa gaacttcaaa
     4501 tacctcatct aatgtaccaa tccccccagg caaagccaca aagacatctg catattccat
     4561 cattagttct tttctttcag ccaaactacc aacaaaaaag acttgatcaa gaactttttg
     4621 ctctactttt tttctaaaag aatctggaat aacgccaatc acactacctc ctgatagttt
     4681 taccgccatc gcacattctt gcatcaaacc catattggct cctccatata ccagagtata
     4741 tgatttttta ccaattactt ctcctagcaa acgagcatct tcacgataca cttcggctat
     4801 ttcattgctt gaagaacaaa agacacacac attcatcaca gttccatttt ttacgcatca
     4861 atgcgtgcat aggtagcatt tttttctata aaatcacgtc ttggagcaac atcatcaccc
     4921 attaacatag aaaaaatatg atctgcttca gctgcatttt ctatggttac ttgtcgcaat
     4981 gttctatttt ctgggtccat tgttgtttgt ttcaattgtt gagcattcat ctctcccaaa
     5041 cctttgtagc gttgtatatg gataccacct tcactcccct tgccatattt ttctacaaaa
     5101 gccaaacgtt gcttttcatc ccaacagtac tcttcaatct ttccttttct acacaaatat
     5161 aagggaggtg tagcaatata cacatatcca ttttctatca aatctttcat atgtcggaaa
     5221 aagaacgtta aaattagcgt agcaatatga ctaccatcaa catcagcatc ggtcatgata
     5281 accactttat gatagcgtaa ttttgataaa tttagtgctt tgctatcttc ttctgtccca
     5341 atggtaactc ccaaagcagt ataaatatta cgaatttctt cgctctcaaa caccttgtgg
     5401 ggcatggctt tttctacgtt taatattttt ccacgcaaag gcaaaatagc ctgtgtagca
     5461 cgatctctac cttgtttggc tgtaccacct gccgaatccc cctctactaa aaacaattcg
     5521 cattttgctg gatccttttc ggcgcaatcg gctaatttac caggcaatcc tgctcccgat
     5581 aatggcgatt ttcgttgtac taaatcgcga gccttacgag ctgcatgacg ggctgtggcg
     5641 gctaaaataa ctttttcgac tatggtgcgg gcttcttttg gatgttcctc taaataattt
     5701 cccaatgctt cgcttacagc catatccacc gaccccatca cctcattatt accaagcttg
     5761 gttttcgtct gaccctcaaa ctgtggctcc gctaccttaa ttgagataac cgccgttaag
     5821 ccctctctaa agtcatttcc gtctatttct attttatttt tttcaagctt ttcgagcaat
     5881 ttattctctt ctgcatattt ctttaatgta cgggtcaatc cacgtctaaa acctgccaaa
     5941 tgagttcccc cctcaatagt attgatatta tttacatatg aatggatatt ttcattgtaa
     6001 gaagtattgt atagcatagc tatttctact ggagttgcat atttatccgt tgatatatga
     6061 atgacttcat caattaactt ctcacgagct tgatctataa atctaacaaa ttctgccaat
     6121 ccttctgttg aataaaattc ttccgatttg tacgacccat cttcaaatct ctcacgttta
     6181 tctgtcaaaa tcaaacggat acctgcattt aaaaatgcta actcacgtaa acgaccagct
     6241 aaaatatcat atttatattc agtagtcata aaaatagatc catctggctt aaaggttaca
     6301 gtagttcctt tctgagtaga cacgccaatt tctctgacag aaaattggg
//
LOCUS       DAOSRP010000104         6348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_11285_length_6348_cov_5.197203, whole genome shotgun sequence.
ACCESSION   DAOSRP010000104 DAOSRP010000000
VERSION     DAOSRP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_6348_cov_5.197203"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..849)
                     /locus_tag="PLJ82_06955"
     CDS             complement(373..849)
                     /locus_tag="PLJ82_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOR41278.1"
                     /translation="MEKLDRIKSPIFLKTALYCSSAERCKSEVIERLKKETNSLDEIE
                     EMVAILEEEGYINELRYAISYVRDKFRFNKWGKIKISYMLQCKKIPSSIITEALAEID
                     DDTYLETLTALVLSKSKSIKSTNPSDKKAKIYRFAASRGFESESIRQALIQCRVDK"
     gene            complement(837..1700)
                     /gene="prmC"
                     /locus_tag="PLJ82_06960"
     CDS             complement(837..1700)
                     /gene="prmC"
                     /locus_tag="PLJ82_06960"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOR41279.1"
                     /translation="MDKIAISDCKLFFTQSLSSLYPPNEIESLFRWTISHVLGIPFSH
                     TIAYKDIKITNTQEIDIKTIVKKLQQTQPIQYIFGETEFFGLSFHVNSDVLIPRPETE
                     ELVEWILADHRQESTSKRILDIGTGSGCIAISLAKNIPTSRVTALDVSHNAIEVAKRN
                     AHNHELTIQFIEKDILHLKNGDLNEQFDVIVSNPPYVCENEKSTINNHVLLHEPHLAL
                     FVPNDNPLQFYEAIALFAHTNLAINGAIYVEINYLFGKETQQLFHQYGFSTHLKKDIA
                     GKDRMIKAWKN"
     gene            1773..2804
                     /gene="ribD"
                     /locus_tag="PLJ82_06965"
     CDS             1773..2804
                     /gene="ribD"
                     /locus_tag="PLJ82_06965"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOR41280.1"
                     /translation="MSIHETYMHRCLQLASLGKGRVAPNPMVGCVIVHNDVIIGEGYH
                     RTYGEAHAEVNAIESVKNKALLKESTLYVSLEPCSHYGKTPPCADLIIQYKIPRVYIG
                     TKDPNPKVSGNGIARLETAGVAVECGILAAQAMEINKYFFTFQLKNRPYITLKWAKSA
                     DGYIDALQPKPIQLSNEITKTLVHKLRSENMAILVGTNTALKDNPSLRTRRWFGKNPV
                     RIGIDKNLRIPKNYLLYNGQTTTLLFNSIKQEEGNPCMIQIDFTKDIIPQLLHELYKR
                     NIHSLIVEGGKILLDSFINTGLFDEIHEETADVLLYEGTCAPQFSESILCSMEIVGKN
                     TCATYVKKE"
     gene            2804..4237
                     /gene="cls"
                     /locus_tag="PLJ82_06970"
     CDS             2804..4237
                     /gene="cls"
                     /locus_tag="PLJ82_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOR41281.1"
                     /translation="MEFLSDILILIFLATYVYTVISSIVVVLLENKHPVRTSAWIMVL
                     IFVPFIGLLLYVVVGRSYHRQGNISKKLKTKILSFGTRQSIKEICAENNICSKHERLI
                     HLLKNSTDASIFTHNSVNIYTDGKQLFDDILSAMDNATAHIHAEYYAVETDETGNRFK
                     DMLIKKSKQGLEVRLIYDDVGSWRLKRSTIQEMKNAGVILQTFFELRFPYFTNKLNYR
                     NHRKILIIDGKIGFLGGVNIADRYTKGLPWGIWRDTHIKIQGNAVDGLQQLFLNDWLY
                     ITKTAIHHKKYFAIHDQYNKLPIQIVGSGPDSMWEGIMQGFCAAIQNASKYVYIQTPY
                     FLPNDSISNALESAALSGIDVRIMIPSRSDTRISLEASLSFVKTMLAAGVRVYQYQAG
                     FIHAKSITIDNELAIIGSANMDFRSFEQNFEVSAFMYDSDKSMELRNIFLHDMKQCKR
                     LQYTQWKHRPITQKLIQSSARLFSPLL"
     gene            4255..4716
                     /locus_tag="PLJ82_06975"
     CDS             4255..4716
                     /locus_tag="PLJ82_06975"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOR41282.1"
                     /translation="MFLVGFMGTGKTTFGKKLSQKIHFSFIDTDLYIENNFHKTINEI
                     FAEKGEEGFRTIETKILAEIAEIENVVIATGGGTPCFNNNMALMNSKGLTIYLETPTP
                     DIAQRLITGKTKRPLLKDKTSEEIVEFIEDILPKRSTYYKKADLTINVVEN"
     gene            complement(4770..5165)
                     /locus_tag="PLJ82_06980"
     CDS             complement(4770..5165)
                     /locus_tag="PLJ82_06980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOR41283.1"
                     /translation="MASIFSKIVQGEIPCHKIAETPDYLAFLDVRPLVRGHVLVIPKQ
                     EVSYIFDLEDNVLSGLHVFAKKIAKAIEKAIPCKRVGVAVIGLEVPHAHIHLIPLTKE
                     SDIYFSNTKLELTDDELASIATSIRTFLA"
     gene            complement(5177..5647)
                     /gene="greA"
                     /locus_tag="PLJ82_06985"
     CDS             complement(5177..5647)
                     /gene="greA"
                     /locus_tag="PLJ82_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOR41284.1"
                     /translation="MSITYMTKEGYKKMMDELNHLESVERPNISMQIAEARDKGDLSE
                     NAEYDAAKEAQGMLELKIAQLKETVKNARIINEKDLNTDYVQILNKVRIRNLKNNQEF
                     TYTLVSEQEASLKEGKIAINTPVAKALLGKKVGEIVEVTVPSGVLRLEIINISF"
     gene            complement(5818..6174)
                     /locus_tag="PLJ82_06990"
     CDS             complement(5818..6174)
                     /locus_tag="PLJ82_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41285.1"
                     /translation="MSISTIIFIVVFILFIIRKLGSKKEVVAQVPPIVHKPKPFLAEN
                     KNKQPHVNNTKSSQATKLNNTSFSDMSTISEKKLPRDEKLHNVVEISTDNDFTLHQED
                     LKKAIVYAEIINRKYE"
BASE COUNT         2142 a         1102 c         1054 g         2050 t
ORIGIN      
        1 cgctcatttc actgcatttt ttggctacat aaacgaggtc gcgtatttag tggaaagttg
       61 aaagtgaaca gttgtaagtt ttgctcgaga aacgctcaac gagcaacgtt caacactaaa
      121 cactttacac ttttaactgg caactatcaa ctaagatgtc agggctaacg cccctaatta
      181 tttgactatt ttatttttct acgaagatta cagagctacg ctcctttttt tagtggaaag
      241 ttgagaattg agaattgata gttcagctcg agtaacgaat aactattaac gataaacgtt
      301 caacactaaa cacttttaac tatcaattgt caactatcaa ttatcaacta tctaactttt
      361 cactttcaac tgtcacttgt caactctgca ctgaatgagg gcttgtcgga tactttcgct
      421 ttcaaaaccc ctgcttgctg caaatctgta tatttttgct tttttatccg atggattggt
      481 tgatttaatg gatttacttt tggagagaac caaggctgtt aaggtctcca gataagtatc
      541 gtcgtctatt tcggcaagag cttctgtaat aattgacgag ggtattttct tacattgtaa
      601 catataggaa atttttattt taccccattt attaaatctg aatttatccc gtacatagct
      661 tatggcataa cgtaattcat tgatatatcc ctcttcttcc agaatagcta ccatttcttc
      721 gatttcatct aaagaattgg tctctttctt taaacgttct attacctcgc ttttgcatct
      781 ttcagccgaa gaacaataga gagctgtttt taaaaaaatg ggggatttta ttctgtctag
      841 tttttccatg cctttatcat tctatccttg ccagcaatat ctttcttaag gtgtgtacta
      901 aagccatact ggtggaaaag ttgttgtgtt tcttttccaa agaggtaatt aatttccaca
      961 tatatagctc catttattgc taaattcgta tgggcaaata gagcaattgc ttcgtaaaac
     1021 tgcaaaggat tatcgttagg aacaaacaaa gccaaatgcg gttcgtgcaa caaaacatga
     1081 ttattgatag tggatttttc gttttcacaa acataaggcg gattactgac aataacatca
     1141 aattgttcgt ttaaatcgcc gttttttaga tgtaatatat ctttctcaat aaactggatt
     1201 gtaagctcat ggttgtgtgc attacgtttt gctacttcaa tggcattgtg cgacacatct
     1261 agagctgtta cacgagaagt tgggatattt tttgctaacg aaatggcgat acatccactt
     1321 ccagtaccta tatcaagtat tcttttggat gtgctttctt gcctatgatc ggctaaaatc
     1381 cattcgacca actcttccgt ttcgggacgt ggtatgagca catccgaatt aacatggaat
     1441 gataatccaa aaaactctgt ttcaccaaaa atatattgta tcggttgtgt ttgttgtaat
     1501 ttttttacaa ttgttttaat atcaatttct tgcgtgttag taatttttat atctttgtat
     1561 gcaatggtat gagagaaagg aatacccaat acatgcgaga ttgtccatcg aaataggctt
     1621 tctatttcat tgggaggata taaagaagaa agactttggg taaaaaacaa tttacaatca
     1681 gagatagcaa ttttatccat tgatatagta ttacaaacga caatacaaaa ctacaaaaat
     1741 ttaacgtaag gacatcattc aagccaaata aaatgagtat tcacgaaaca tatatgcacc
     1801 gttgcttgca attagcctcc ttaggcaaag gcagagtagc tcccaatcca atggttggat
     1861 gtgttattgt acataacgat gtaattatag gagaaggata ccatcgtacg tatggagaag
     1921 cacatgcaga agtaaacgcc attgaatcgg taaagaataa ggcattatta aaagaatcaa
     1981 cactttatgt tagcttagaa ccctgttcgc attacggtaa aactccgcct tgtgcagacc
     2041 ttatcatcca gtataaaatt cctcgtgtat atattggaac gaaagaccct aacccaaaag
     2101 tatcaggcaa tggtattgca cgtttggaga cagcaggtgt tgctgtggaa tgtgggatat
     2161 tagcagcaca ggcaatggaa atcaacaaat atttttttac atttcaacta aaaaaccgcc
     2221 catatattac gttgaaatgg gcaaaaagtg ccgatggata tattgatgcc ctacaaccaa
     2281 agccaataca actgtcaaat gaaataacaa aaacactggt tcataaatta cgttctgaaa
     2341 atatggcgat attggttggc acaaatacag cattaaaaga caatccatcg ctacgaacta
     2401 ggcggtggtt tggaaaaaac ccagtacgta taggtataga taaaaattta cgtattccta
     2461 aaaattactt gctttacaat gggcaaacaa caaccttact ttttaactca atcaagcaag
     2521 aagaaggtaa cccgtgtatg atacaaatag actttaccaa agatattata ccccaactgt
     2581 tacacgaatt gtataaacgg aacatccatt cgttgattgt agagggcggt aaaattttat
     2641 tagactcctt tattaacaca ggattatttg atgaaattca cgaagaaacc gcagatgttt
     2701 tattgtatga aggtacttgt gctccacaat tttccgaatc aattttatgt tctatggaaa
     2761 tagttggcaa aaatacatgt gcaacttacg taaaaaaaga ataatggaat ttttatccga
     2821 catactcata ttgatattcc ttgctacgta tgtgtatacg gtgatatcaa gcattgtggt
     2881 tgtattgtta gaaaacaagc atcctgttcg tacttcggcg tggattatgg tacttatttt
     2941 tgttccattt ataggactat tattatatgt tgttgtggga agaagttatc atagacaagg
     3001 aaatatatca aaaaagctaa aaaccaaaat tttatctttt gggacaaggc aatctattaa
     3061 agaaatttgt gctgaaaata acatttgtag taaacacgaa agattaattc atttattgaa
     3121 aaacagtaca gatgcttcca tatttacaca taatagtgtc aatatttata ccgatgggaa
     3181 acaattattt gacgatatac tcagtgctat ggataacgct accgcacata tccatgccga
     3241 atattatgcc gttgaaaccg atgaaacagg caatcgcttt aaagatatgc ttatcaaaaa
     3301 atcgaaacaa ggactggaag tgcgattaat atatgacgat gttggtagct ggcgattaaa
     3361 acgatccacc atacaagaga tgaaaaatgc aggcgtgata ttgcaaacat ttttcgaatt
     3421 acgattcccc tattttacaa ataaattaaa ttatcgtaac caccgaaaga tactaatcat
     3481 tgatggaaaa attggctttt taggaggtgt aaatattgca gatagatata caaaaggctt
     3541 gccttgggga atatggaggg atacgcatat caaaatacaa ggaaatgcag tagatggatt
     3601 acaacaatta tttctgaatg actggttata tattaccaaa acagcaatac atcacaaaaa
     3661 atattttgct atacacgatc aatacaataa actacccatt caaatagtag gaagtggtcc
     3721 agattcgatg tgggaaggga taatgcaagg attttgtgcc gctatacaaa acgcttccaa
     3781 atatgtgtat atacaaacac cttatttttt acctaatgac agcatatcca atgcattaga
     3841 atctgcagca ttatctggaa tagacgttag aattatgatt ccaagtcgtt ctgacacccg
     3901 tatatcatta gaagcttctt tatctttcgt taaaaccatg ctagctgctg gagtgcgtgt
     3961 atatcaatat caagcaggtt ttattcacgc aaaaagcatt actattgaca atgaacttgc
     4021 aattatcgga agtgcaaaca tggattttag gagttttgaa caaaacttcg aagtaagtgc
     4081 atttatgtat gactcagaca aaagcatgga actacgtaat atctttttac acgatatgaa
     4141 acaatgtaaa cgtttacaat atacacaatg gaaacatcgc ccaataactc aaaagttgat
     4201 acaatcatca gctcgattat tcagcccgct cttatagcaa aaaactgtac cagaattttt
     4261 ttagttggct ttatgggcac tggaaaaaca acatttggga aaaaactatc ccaaaaaatt
     4321 catttttcgt ttatcgatac agatttatat atcgaaaata attttcataa aaccattaat
     4381 gaaatttttg ctgaaaaagg agaagaaggg tttcgcacaa ttgagacgaa aatattagca
     4441 gaaattgcgg aaattgaaaa cgtagtaatt gcaacaggag gaggtactcc gtgctttaac
     4501 aataatatgg cattgatgaa tagtaaagga ttaaccatct atttagaaac acccactcct
     4561 gatattgcac aacgtttgat tacaggaaaa acaaaacgac ctttattaaa agataaaact
     4621 tctgaagaaa tagtggaatt tatagaagat atactaccta aaagaagtac gtattacaaa
     4681 aaagccgatc tcactattaa tgtggttgaa aattagaaga ggaaagtctt ataaactctc
     4741 ctcctctgtc aattatattt tagctccctt taggcaagaa atgttctaat ggaagtggca
     4801 atagaagcta attcatcatc tgtaagctct aattttgtat tagaaaaata gatatctgat
     4861 tcttttgtca acggaattaa atgaatatga gcatgtggca cttctaaacc aattacagca
     4921 acaccaaccc ttttgcatgg aatagccttt tcaatagctt ttgcaatttt ttttgcaaat
     4981 acatgtaacc cactcagcac attatcttcc aaatcaaaaa tatacgacac ttcctgtttt
     5041 ggaatcacca atacatgccc cctcactaac ggacgaacat ctaaaaatgc tagataatct
     5101 ggggtttctg ctatcttatg acaaggaatt tctccttgaa caattttact aaaaatacta
     5161 gccatagtat atctttttaa aaagaaatat ttattatttc caatcgtaat acaccagaag
     5221 gaactgttac ttcgactatc tctcctactt tttttcctaa taatgcctta gccactggag
     5281 tgttaatcgc tatctttcct tctttcaaac ttgcttcttg ctcagacaca agagtataag
     5341 taaactcctg gttatttttc aaattacgta tacgtacttt gtttaaaatc tgaacataat
     5401 ctgtatttaa atctttttca ttgataatac gagcattttt cactgtttct tttagctgag
     5461 caattttcaa ttccagcatt ccttgagcct cttttgccgc atcatattca gcattttcag
     5521 acaagtctcc cttgtcacga gcctctgcaa tttgcatcga aatgttaggg cgttccaccg
     5581 attccaaatg atttagttca tccatcattt tcttgtaccc ttctttggtc atgtacgtaa
     5641 tactcataat aaaataattt agattaaaaa aatagtcctt taacgtattt acataaataa
     5701 agagaatctc gaaattaatc gagactctct ggtgtgtttg taattacttc ggcaaagata
     5761 aagctttctt ttcaaagaaa aaaatatttc atcgaataaa tctttgagct aagaattcta
     5821 ttcgtacttc ctgttgataa tttcggcata cacaattgcc tttttcaaat cctcctgatg
     5881 taaggtaaaa tcattatcag tactaatttc gacaacatta tgtagttttt catctcgagg
     5941 cagttttttt tctgatatcg ttgacatatc agaaaaactt gtgttgttta atttagtcgc
     6001 ttgggaagat ttagtgttat taacatgtgg ttgtttgttt ttattttcag ccaaaaatgg
     6061 ttttggctta tgcacaatag gaggaacctg tgctactact tcttttttac tccccaactt
     6121 acgtattatg aatagaataa aaacaacgat aaaaataatg gtggatattg acattgtatt
     6181 tgtgtataat gaatgtgtac tacaaagata ataaaaaatt aactagatac caaaagttaa
     6241 aaggagagcc gctgcccccc ttttaaccta acctaaacct taactatgaa aaaaatcatt
     6301 gtttcttttt cttgatacaa agaaaagcat ttgttttgaa ttacgcaa
//
LOCUS       DAOSRP010000105         6225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_11579_length_6225_cov_5.712804, whole genome shotgun sequence.
ACCESSION   DAOSRP010000105 DAOSRP010000000
VERSION     DAOSRP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6225_cov_5.712804"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLJ82_06995"
     CDS             <1..474
                     /locus_tag="PLJ82_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOR41286.1"
                     /translation="DMVAAALKWNGGFVWACKNYDGDVQSDTVAQGFGSLGLMSSVLV
                     TPDGKTVEAEAAHGTVTRHYRQHQQGKETSTNPIASIFAWTRGLAHRGKLDNNQALVN
                     FCTTLEKVCVETVESGKMTKDLAILVSGDNVSRKDYLSTQEFLAAIKENLDKALA"
     gene            588..3380
                     /gene="polA"
                     /locus_tag="PLJ82_07000"
     CDS             588..3380
                     /gene="polA"
                     /locus_tag="PLJ82_07000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR41287.1"
                     /translation="MKKLFLLDAYALVYRAYYAFIKNPRINSKGLNTSAIYGFVNTLE
                     DILKREKPSHIAIAFDPSGKTFRHEAYEAYKAQRESTPEDIRTSLPIIKNIIKAYGIP
                     IIEVPGFEADDVIGTLAKKAEKAGFITYMVTPDKDYGQLVSERIFMYRPKHTGGYETL
                     GIEEIKEKYQLASHEQVIDLLGLMGDSSDNIPGCPGVGEKTAVKLLQEFGSIDNLLAH
                     TEQLKGALKTKVEENKDLIAFSKFLATIRTDVPVELHESELILSEPDENQLRTLYEEL
                     EFRTFIAKMQASQASKPETSVSKKAPQQGNLFDMFEENDAEEERTAMGSTSTMDTIDT
                     IKPTYILLNNAKDIDAFVADLLSQKTVCFDTETTGIDINESKLVGMSFCWKKNTAYYI
                     PFTSFDETALFLEKIQPLFNNEHTLLIGQNLKFDLLMLQNYGMSVRAKLFDTMVAHYV
                     LQPELRHGMDYLAEIYLKYRTIHYEDVVGVKGKNQQNIQDIDPAIVCNYAAEDADITF
                     QLKEILEKELHANRLEKLFFDIEMPLVKTLCTIERAGVRIDTQALSQSSLVLTEQMQE
                     IEHNVQQHVGFEFNLSSAKQVGEVLFDRLKITDKAKKTKTGQYATSEDILEELRGKHP
                     IVPLILEYRGLKKLLSTYIDALPLLVNKHSGKIHTSYNQTITATGRLSSSNPNLQNIP
                     IRDAQGKEIRKAFIPDEGCLFLSADYSQIELRIMAHLSQDENMVDAFLSGHDIHAATA
                     AKIYKIPIEEVSSDMRRKAKTANFGIIYGISVFGLSDRLSISRSEAKELIDGYFDTYP
                     GVKSYMEASIAKAKEQGYVETLLGRKRFLPDINSSNSFVRGFAERNAINAPIQGSAAD
                     IIKIAMIRITQRFEQENIQSQMTMQVHDELNFSVLKSELEIVKRIVIEEMEHAISLRV
                     PLVADCGVGDNWLEAH"
     gene            complement(3493..3909)
                     /gene="tsaE"
                     /locus_tag="PLJ82_07005"
     CDS             complement(3493..3909)
                     /gene="tsaE"
                     /locus_tag="PLJ82_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR41288.1"
                     /translation="MPQLQIPSLQELPVVATTFIRLLPKDTRIIAFEAGMGVGKTTFI
                     KAICQVLGVQDVVNSPTFSIVNEYACSNGESVYHFDFYRLQSPQEALDFGVYDYFESG
                     NWCFMEWAENIAPLLPTETLFVTLTENPDGSRDIRF"
     gene            complement(3923..>6225)
                     /locus_tag="PLJ82_07010"
     CDS             complement(3923..>6225)
                     /locus_tag="PLJ82_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOR41289.1"
                     /translation="TLTGDRAAAPRYIEARLSKFALEVVFNPKTTEWKLSYDGRNREP
                     VTLPVKFPLLLAQGIEGIAVGLSSKILPHNFNELIDASIAHLKGEDFEIFPDFIGGGY
                     IDVSRYNQGERGGSVKVRAKISKLDSKTLVIQELPFGETTSSLIDSIVKASEKGKIHI
                     KRVDDNTSANVEILVHLAPGKSSDKTIDALYAFTNCEVSISPNCCVIYEDKPHFLTVN
                     TVLKHSTDHTLNLLKEELLLQKAELQESIFFSSLEKIFIEQRIYKDKEFEESKNLAEA
                     VAHIDKRLVPFKKLFIREVETEDIERLMEIKMGRILKFNSDKADQHIANLNEQLEQVK
                     HHLAHIVEYTISWFVGLKEKYGHLYPRKTEIRNFDTIEVTKVVEANQKLYFNREEGFI
                     GTGLKKDEFVCNCSDIDDIILIYKDGKYKVTKVAEKLYVGKNVLYINVFKRNDNRTIY
                     NVVYRNGKDGVSYIKRFAITGVTRDKEYDITQGEAGTKIIYFSANPNGEAEVLKVTLK
                     PKPRLKSLVFERDFSDIAIKGRMSMGNIFTKNDIHKIVLKQKGGSTLGGREVWFDKDV
                     MRLNYDGRGVSLGEFDSDDSILVLSKDGCFYTTNFELSNHFEDNVLLLEKFDEDKVWS
                     AVLYDAEQQFYYVKRFTLEQTNKKTSFIGDNPESRLLLMSTQVYPRLQVLFGGKDAER
                     EPQEIDVEEFIGVKSFKAKGKRLTTFEIKDIVELEPTRFPEVADEEPENTDEIEENDG
                     NEKVDNEEVIDKKKIIEDITGQMSLF"
BASE COUNT         2037 a         1264 c         1091 g         1833 t
ORIGIN      
        1 gatatggtag cagctgcact aaaatggaac ggcggatttg tgtgggcttg taaaaattac
       61 gatggcgacg tgcaatcaga cactgtagca cagggttttg gttcgctagg tttgatgtcg
      121 tcggtattgg taactcccga cgggaaaacc gtagaagcag aggcagctca tggcaccgta
      181 acgcgccatt atcgtcagca ccaacaaggc aaagaaacat caacaaatcc tattgcatca
      241 atttttgcat ggacgcgtgg attagctcat agaggaaaac tagacaacaa tcaagccttg
      301 gtaaacttct gtactacact agaaaaagta tgtgttgaaa cagtagaatc aggcaaaatg
      361 acgaaggatt tagctatctt agtttctgga gataatgttt ctcgcaaaga ttatttaagc
      421 acacaagaat ttttggctgc aattaaagaa aacctagaca aagctttagc ataagcttta
      481 ctaaattttt atcatagata ttatgggagg ggctgtttgt ttctcccatt tttgtttgta
      541 tctttgtaag atatgaactc ataggatatt cacctgatac aattacaatg aagaaattat
      601 ttcttttaga cgcttatgcc cttgtttaca gagcgtatta tgcgtttatt aaaaaccctc
      661 gcatcaactc aaaaggactc aacacctcag caatttacgg ttttgttaac accttagaag
      721 acattctgaa aagagaaaaa ccaagccata tagcaatagc tttcgaccca tctggcaaga
      781 cttttagaca cgaggcttac gaggcataca aggctcaacg agaatcaact cccgaagata
      841 ttcgcacgtc gttgcccatt ataaaaaata taattaaagc gtatggtatt cctattattg
      901 aggtgcctgg ttttgaagca gacgacgtaa ttggtactct cgcaaaaaaa gcagaaaaag
      961 cgggttttat tacctatatg gtaactcccg acaaagatta tgggcaactt gtgtctgaac
     1021 gtatttttat gtaccgaccc aaacatacgg gagggtatga aactctagga atagaagaaa
     1081 taaaagaaaa ataccaatta gcttctcacg aacaagtaat agatttgctc ggattgatgg
     1141 gcgatagtag cgataatata ccgggctgcc ctggcgtggg cgaaaaaacc gccgtcaaac
     1201 tgttacaaga atttggtagt attgacaatc tgttggctca tactgaacag ctgaaaggtg
     1261 ccttaaaaac gaaagtggaa gaaaacaagg acttaatcgc tttctcaaaa tttttagcca
     1321 caatacgtac cgacgtacct gttgaattac atgaatcgga acttatactg agcgaaccag
     1381 acgaaaatca attgcgtaca ctatacgaag aacttgagtt tcgcaccttt attgcaaaaa
     1441 tgcaggcatc acaagccagt aagccagaaa caagtgtttc aaaaaaagct cctcagcaag
     1501 gcaatttgtt tgacatgttt gaggaaaatg atgctgaaga agaacgtaca gcaatgggat
     1561 caacttcaac aatggatacc atcgatacaa ttaaacctac ctatatttta ctgaataatg
     1621 cgaaagatat agatgccttt gtagcggatt tattgtctca aaaaacggtt tgttttgata
     1681 cagaaacaac aggcatagat atcaatgaga gcaaactagt aggcatgtcg ttttgttgga
     1741 aaaaaaatac tgcttactac atacctttta cttctttcga tgaaaccgca ctttttttag
     1801 aaaaaattca gccactattt aacaacgaac ataccctttt aataggacaa aacctaaaat
     1861 ttgacctatt aatgttgcaa aattacggca tgtctgtacg tgctaaacta ttcgacacga
     1921 tggtagctca ttatgtactc caacccgaat tgagacatgg gatggattac ttagccgaaa
     1981 tttacctcaa ataccgcaca attcactacg aagatgtagt aggagttaaa ggaaaaaacc
     2041 aacaaaatat acaagatatt gaccctgcta tagtatgtaa ttatgcagca gaagatgccg
     2101 acataacttt tcagctaaaa gaaatactcg aaaaagagct tcatgccaat cgcttagaaa
     2161 aacttttctt cgatatcgaa atgcctttag taaaaaccct atgtactatc gaacgagcag
     2221 gtgtacgtat tgatacccaa gcattgtctc aatcttcgtt ggtacttacc gaacaaatgc
     2281 aagaaattga acacaacgta caacaacacg tgggatttga atttaatctt agttcggcta
     2341 aacaagttgg agaagtattg ttcgaccgat tgaaaattac agataaagcg aagaaaacaa
     2401 agacagggca atatgccact tctgaagata ttttagaaga attacgaggc aaacatccta
     2461 ttgtccctct catactcgaa taccgaggat taaaaaaact ccttagcacg tatattgatg
     2521 ctttgcctct attagtaaac aaacattcag gaaaaataca cacttcttac aatcaaacaa
     2581 ttacagctac ggggcgacta agttcgagca atccgaacct gcaaaatata cccatacgag
     2641 atgctcaagg caaagaaata agaaaagcat ttatccccga tgaggggtgt ttattccttt
     2701 ctgcggatta ttcgcaaatt gaattgcgga ttatggcaca tttgagtcaa gatgaaaata
     2761 tggtagacgc atttctttca ggacacgaca tccatgcggc tacagcggcg aaaatttaca
     2821 aaattccaat cgaagaagtg agcagtgata tgcgacgcaa agccaaaaca gcaaattttg
     2881 gtatcattta cggtatctcg gtatttggac tcagcgaccg actcagcatc tcacgcagcg
     2941 aagccaaaga actcatagat gggtattttg atacttaccc aggagtaaaa tcatacatgg
     3001 aagcgagcat tgcgaaagcc aaagaacaag gatacgtaga aacactttta ggtcgtaaac
     3061 ggtttttacc tgatatcaat tcctccaata gttttgtacg tggctttgcc gaacgaaatg
     3121 ctatcaatgc acccattcaa ggctctgctg ccgatataat caaaatagcc atgatacgta
     3181 ttacacaacg atttgaacaa gaaaatatac aatcacaaat gaccatgcag gtgcatgatg
     3241 agctgaactt tagcgtgtta aaaagtgaac tggaaatcgt aaaacgtatc gtaattgaag
     3301 agatggaaca tgctatttca ctacgagtac ctttagtggc tgattgtggt gtaggtgata
     3361 attggttaga ggcacattaa taattttctc cccaccaaaa ctccagaggg gcgatataat
     3421 aatagaataa aaacacagca aacaaagtaa gctccatagg agcgacataa aaaaccccaa
     3481 tacatacatt tattaaaacc gaatatccct gcttccatcg ggattttccg ttagggttac
     3541 gaaaagagtt tccgtcggta ataagggggc aatattttct gcccattcca tgaaacacca
     3601 attgccactt tcaaaataat cgtacactcc aaaatccaat gcctcttggg gagattgtaa
     3661 tcggtaaaaa tcaaaatgat atacagattc gccattgcta caagcgtatt cattcacgat
     3721 agaaaacgta ggactattga caacatcttg cacacccaac acctgacaaa tagctttgat
     3781 aaaagtagtt ttacctaccc ccatacctgc ttcaaaagct atgatacggg tatctttggg
     3841 aagtaagcgt ataaaagtag tagctactac tggaagttcc tgcaacgatg gtatttgaag
     3901 ttgtggcata aaggagattt tattaaaaca aactcatctg accagtaata tcttcaatta
     3961 ttttcttttt gtctattact tcttcgttat ctactttttc atttccatca ttttcctcga
     4021 tttcatcagt attttcaggc tcttcatcag ctacttctgg aaaacgtgtt ggctccaact
     4081 ctacaatatc tttgatttca aatgtagtta aacgtttccc tttggcttta aaacttttca
     4141 cgccaatgaa ctcttctaca tcaatttctt gcggttcacg ctcggcatct ttcccaccaa
     4201 acaatacttg caaacgtgga tatacctgcg tagacatcag caacaaacgt gattcgggat
     4261 tatctccaat aaatgatgtt tttttgttgg tttgttctaa agtaaaacgt tttacataat
     4321 aaaactgttg ctcagcatcg tacagtacag ctgaccacac cttatcttca tcaaattttt
     4381 cgagtaacaa gacattatct tcaaaatgat tactcaattc aaaatttgta gtataaaaac
     4441 aaccgtcctt ggataatacc aaaatcgaat catcactatc aaattcgcca agcgatacgc
     4501 ctcttccatc ataatttaat cgcatcacat ctttatcaaa ccacacttcg cgacccccta
     4561 aggtagaacc gcctttttgt tttaatacaa tcttatgaat atcgtttttg gtaaaaatat
     4621 ttcccatcga catacgtcct ttgatggcaa tatcactaaa gtcgcgttca aaaaccaacg
     4681 atttcaacct aggttttggc tttaaggtaa cttttaatac ctccgcttct ccattcggat
     4741 tggcactaaa atagatgatt tttgtaccag cctctccttg ggtaatatcg tactctttgt
     4801 cgcgtgttac acccgtaatg gcaaaacgtt tgatgtaact cacaccatct tttccattgc
     4861 gatataccac attgtatata gtacggttgt cattccgttt aaacacattg atatacagca
     4921 catttttgcc cacatacaat ttttcagcta ctttagttac cttgtatttt ccatctttgt
     4981 aaatcaagat aatatcatca atatccgaac aattacacac aaactcatcc ttttttaagc
     5041 ctgttccgat aaaaccttct tcgcgattaa aatacaattt ctgattggct tcaaccactt
     5101 tggttacctc tatggtatcg aaattacgaa tttcagtttt acgaggatat aaatgaccat
     5161 atttctcttt taatccaaca aaccaactga ttgtgtattc cacaatatga gccaaatggt
     5221 gttttacttg ctccaattgt tcatttaggt tcgctatatg ctggtcagcc ttatcagaat
     5281 taaatttgag gatacgcccc atttttattt ccatcaaacg ctcaatatct tctgtttcta
     5341 cttcgcggat aaatagcttt ttaaaaggga ctaatcgttt atcaatatga gctactgcct
     5401 cagccaggtt tttactttct tcaaactctt tatctttata aatacgttgt tcgataaata
     5461 ttttttcgag cgaagagaaa aagatacttt cttgcagttc agctttttgt agcaacaact
     5521 cctctttcaa caagttgaga gtatgatctg tagaatgttt tagtacagta ttaacggtta
     5581 aaaaatgtgg tttatcttcg tatataacac aacaattggg cgatatactc acttcacaat
     5641 tcgtgaatgc gtacaaggca tcaatggttt tgtccgatga ttttcctgga gccaaatgca
     5701 ctaaaatttc cacattagca gaagtattat cgtccaccct tttgatatga attttgcctt
     5761 tttccgatgc ttttacaatc gagtcaatca acgaactagt cgtttcgcca aatggtaatt
     5821 cttgtattac cagtgtttta ctatctaatt tgctaatttt agcacgcact tttacacttc
     5881 cgccacgctc gccttggttg taccgactca catcaatata tccacccccg ataaaatcag
     5941 gaaaaatttc aaaatcctcg cctttgagat gggcaataga agcgtcaatt aactcattaa
     6001 aattgtgtgg caatattttt gaagacaatc ctactgcaat accctctatg ccttgagcta
     6061 ataacaaagg gaatttaacg ggtaaagtta caggctctct gtttcttcca tcatacgaca
     6121 atttccactc ggtagttttt gggttaaaca ccacttcgag ggcaaattta ctcaaacgag
     6181 cttcgatata acgaggagct gctgccctat cgccagtaag cgtat
//
LOCUS       DAOSRP010000106         6208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_11615_length_6208_cov_5.851455, whole genome shotgun sequence.
ACCESSION   DAOSRP010000106 DAOSRP010000000
VERSION     DAOSRP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11615_length_6208_cov_5.851455"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..314)
                     /locus_tag="PLJ82_07015"
     CDS             complement(24..314)
                     /locus_tag="PLJ82_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOR41290.1"
                     /translation="MAYGGQGTIILSDGTKVWLNAGSTLTYDDDFSNNRLVYLDGEAY
                     FEVTKHKGKQIFCQNQIGQNTLLFLLPQKMQTIYHFRLQMNAYGIIQKGMIL"
     gene            complement(462..677)
                     /locus_tag="PLJ82_07020"
     CDS             complement(462..677)
                     /locus_tag="PLJ82_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41291.1"
                     /translation="MSTHDNLKSLAEAYFEGKISLEEERQLFAFINDNDDKKKQFYEY
                     EENWKKHYASEDTIKAWQKVYNRMQVA"
     gene            complement(777..1343)
                     /locus_tag="PLJ82_07025"
     CDS             complement(777..1343)
                     /locus_tag="PLJ82_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOR41292.1"
                     /translation="MNFSDPHILLFEIQKGNHLAFEFLFKAYYPRLCNFAARFVDSTT
                     AEDIVQECFLKFWEKRFAIKQGNILSLLFTMVRNACLNHLKHKALVDISSIDFVKNLK
                     GEEALYALDFTAGADYVCLEHELAEQINKVMNDLPARCREVFVLSRMDGLKNKEIALQ
                     LNISTTMVEKHIAKAIAVFSAKFKDDIG"
     gene            1715..3973
                     /locus_tag="PLJ82_07030"
     CDS             1715..3973
                     /locus_tag="PLJ82_07030"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOR41293.1"
                     /translation="MVFDEKMIEEVYKNLSSKLETVQQTLQRPLTLTEKILYTHLSTE
                     EQLRLYERAVDYVNFSPDRVAMQDATAQMALLQLMNSGRKRVAVPSTVHCDHLILAHI
                     AAHVDLTTANESNKEVYDFLKAVSNKFGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTPNAGGLGMIAIGVGGADAVDVMAGMPWELKMPKIIGVKLTGRMSGWTSPKDVILK
                     LAGILTVKGGTNAIIEYFGEGTETISATGKGTICNMGAEVGATTSIFPFDEKMATYLQ
                     ATGRESVAALATKYKKHLQADTEVMANPENYYDRVIEINLNELEPYVNGPFTPDAAYP
                     LSEFAKIVKEKNYPQEMEVGLIGSCTNSSYEDLARAASVVAKAKETNIPVKAKLIINP
                     GSELVRYTAERDGLLETFESVGGVIMANACGPCIGQWKRESTTPDRPNSIVTSFNRNF
                     AKRNDGNPNTHSFVASPEIVAALTIAGDLCFNPLKDKLSTKNGELVKLPEPFGDELPK
                     KGFEVKDAGYIAPSENPDQAVVEIDPQSKRLQVLEAFSAWDGKDIIKQPLLIKVKGKC
                     TTDHISMAGPWLRYRGHLDNISDNMLIGAVNAFTDKTNAVLDTETGDYIAVPALARKY
                     KTAGIGSIVVAEDNYGEGSSREHAAMEPRFLNVKAIVVKSFARIHETNLKKQGLLALT
                     FVNKDDYSKIRENDTISILGVTNMKPGVPLTMVLHHVDGTEESFDVAHTYNLAQINWY
                     KSGSALNALNKI"
     gene            3988..5343
                     /locus_tag="PLJ82_07035"
     CDS             3988..5343
                     /locus_tag="PLJ82_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOR41294.1"
                     /translation="MEKEYLVYKLSETIKTTTRIDRELFTSYNVKRGLRNEDHSGVLV
                     GLTNIGDVVGYERLPEGGLKAIPGKLFYRGIDVEDLAKGIQKDNRVGFEETAFLLLSG
                     YLPDKEELDTFSSILNDSMVLEKKMKMNILELEGQNIMNILARSVLEMYTFDENPDDI
                     SRDNLIRQSIDLIAKFPTIIAYAYNMMRHSTHCRSLHIRHPKDKVSLAENFLYMMQGP
                     RSYSDLDVKILDLALILHAEHGGGNNSTFTVRVTSSSETDTYSSIAAGIGSLKGPLHG
                     GANLQVVDMLEHLKEAISDWTDAAELDAYLQKMLNKEVYNKTGKIYGIGHAIYTISDP
                     RALLLKEMARKLAEEKGLQKEFAFLELIENRAIETFMNFKGERINKQVCVNVDFYSGF
                     VYDAIGLPREVYTPLFAMSRIAGWTAHRIEELNFASKRIIRPAYKNVGGKKEFIPIQK
                     R"
     gene            5383..>6208
                     /locus_tag="PLJ82_07040"
     CDS             5383..>6208
                     /locus_tag="PLJ82_07040"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOR41295.1"
                     /translation="MQKITVQNPVVELDGDEMTRIIWSFIKEQLILPYLNVDIKYYDL
                     GMENRDATNDEVTVEAANAIKKYNVGIKCATITPDEARVVEFGLKKMWKSPNGTLRNI
                     IGGTVFREPIICSNVPRLVPNWTQPIVIGRHAFGDQYKATDVVIKGKGKLTMSFTNEN
                     GETTQWNVYDFEGDGVAMCMYNTDDSIYGFARSSFQMALNKKWPLYLSTKNTILKAYD
                     GRFKDIFQDVYEKEFKADFEANGLVYEHRLIDDMVAAALKWNGGFVWACKNYDGDVQ"
BASE COUNT         2120 a         1128 c         1140 g         1820 t
ORIGIN      
        1 agcttgaaat aagatatcat ctatcacaaa atcattccct tctgtattat tccgtacgcg
       61 ttcatttgta agcgaaagtg atatattgtc tgcatcttct gtggcagtaa aaaaagtaac
      121 gtattctgtc caatctgatt ctgacaaaat atttgtttcc ctttgtgctt tgtaacctca
      181 aagtaggctt cgccatctaa ataaactaat cggttattgc taaaatcatc gtcatacgtt
      241 aaggttgatc cagcatttaa ccacactttt gttccatcag agagaataat tgttccttga
      301 cctccgtaag ccaccctaaa ttcatatcgt tctttttctc cccaagtacc agaatgcaag
      361 aaaaatagtc ccgaaaagcc aattaacaat attagtactg ccgcagcaga caatagtttc
      421 caagaaaaac gagactgaga tactgaagga agaactactg tttatgcaac ttgcatacga
      481 ttatatactt tttgccaagc ctttattgta tcttcactag cgtagtgttt tttccaattc
      541 tcctcatatt cgtaaaattg ctttttctta tcatcgttgt cattgatgaa agcaaacaat
      601 tgacgttctt cttctaaact tatttttcct tcaaaatacg cttcagctaa actcttgagg
      661 ttgtcatgtg tactcataat tatactatct tgtatatata tagcaatcaa tgtcgtattt
      721 tacccaacct aaacaacgtt aaacaacgtt aaaacggcac tagtatctgc cattagctag
      781 ccaatatcat ctttaaactt tgcagaaaaa acggctattg ccttggcaat atgtttttca
      841 accattgttg tagaaatatt taattgaaga gctatctctt tattttttaa tccatccatt
      901 ctgcttaaaa caaacacctc tctacaccga gcaggtaaat cattcattac cttattaatc
      961 tgctctgcta gttcgtgttc caaacaaaca taatctgctc ctgccgtaaa atccaatgca
     1021 tacaaagcct cttctccctt caaatttttc acaaaatcta ttgaagatat atcaactaga
     1081 gctttatgtt ttaagtgatt taggcatgca ttgcgaacca tcgtaaataa taaagagagg
     1141 atattgcctt gttttatagc aaatctcttt tcccaaaatt tcagaaagca ctcttgaaca
     1201 atatcttctg cagtagtaga atctacaaat cgagctgcaa aattacacaa acgaggataa
     1261 tatgccttga ataaaaattc aaaagccaaa tgattgcctt tttgaatttc gaacaacaat
     1321 atgtgtggat ctgaaaaatt catagtgtga tttagatttt acaaaggtaa caattttaca
     1381 aaataaatga ttctttcaag ctattttttt cgcataacaa tggacttcaa cgaaaactat
     1441 gttctcctat actcattcta ttcatatttt tataccataa tattgattat aaaattgagc
     1501 tctcattaaa caataattaa tcgaaacaag tatctattat ttgttatcac atgactctca
     1561 ttttgtatat attccgcata tatacaaaat aaaacactaa aaactctaaa ttgctacatt
     1621 ttttcataca aaaaagcact tctttctatt ttctcatagt ttttttagta tatttgcaaa
     1681 aacgaaaaat ttaactacaa actgaaagaa taatatggtt tttgatgaga aaatgattga
     1741 agaagtatat aaaaacttgt catcaaagct tgaaacagta caacaaacac tacaacgtcc
     1801 tctcacctta acagaaaaaa ttttatatac tcacctctca accgaggaac aattacgact
     1861 atatgaaaga gccgtggatt atgtaaattt ctctcccgac cgtgtagcca tgcaagatgc
     1921 tactgcacaa atggcattgt tacaattaat gaattccgga cgcaaaagag tagccgttcc
     1981 gtctacagta cattgcgacc acttaatact cgctcacatt gctgctcatg tagatttaac
     2041 tacagccaat gagtcgaaca aagaagtata tgatttttta aaagctgtat ccaacaaatt
     2101 tggtattggc ttttggaaac ctggtgcagg cataatacac caagtggtat tagaaaacta
     2161 tgcgtttcca ggaggaatga tgataggaac agactcgcat acacccaatg caggtggttt
     2221 gggaatgatt gccattggcg tgggaggagc tgatgctgtt gacgtaatgg caggtatgcc
     2281 ttgggaactg aaaatgccta aaatcattgg agtgaaatta actggaagaa tgtctggctg
     2341 gacttcaccc aaagatgtaa tccttaaact tgctggtata cttactgtaa aaggaggaac
     2401 caatgccatc attgaatatt ttggcgaagg cacagaaacc atctctgcta cgggcaaagg
     2461 cacaatttgt aatatgggag cagaagtagg tgccacaacc tccattttcc cttttgatga
     2521 aaaaatggct acatacttac aagccacagg cagggaatct gttgctgcat tagctaccaa
     2581 atacaaaaag catttgcaag ctgatacaga agttatggct aatccagaaa attattacga
     2641 tagagtcatt gaaattaact taaacgaatt agaaccatat gttaatggtc cgtttactcc
     2701 agatgcagcc tatcccttgt ctgaatttgc aaaaattgta aaagaaaaaa attatccaca
     2761 agaaatggaa gttggactca tcggttcatg taccaattcg tcatacgagg atttagctag
     2821 ggcagcatcg gtagtagcaa aagcgaaaga aacaaacatt ccggtaaaag caaagttaat
     2881 cattaatcca ggttctgagt tggtgcgtta taccgccgaa agagatggac tattggaaac
     2941 attcgagagt gtaggtggtg ttattatggc aaacgcttgt ggtccatgta ttggtcagtg
     3001 gaaacgtgaa tcaacaactc ctgatagacc caattccatt gtaacttcat tcaacagaaa
     3061 tttcgcaaaa agaaatgatg ggaatcctaa tacacatagc tttgttgcat cacccgaaat
     3121 agtagcggca cttacaatag ctggtgattt gtgttttaat ccgttgaaag acaaactttc
     3181 tactaagaat ggagaacttg taaaacttcc cgaacctttt ggcgatgaac ttcctaagaa
     3241 agggtttgaa gtaaaagatg caggctatat agctccctct gaaaatcctg atcaagctgt
     3301 tgtagaaata gacccccaat caaaacgatt acaagtatta gaagcatttt cagcttggga
     3361 tggaaaagac attataaaac aacctttact catcaaagtg aaagggaaat gcaccaccga
     3421 ccacatctcg atggctggtc cttggttgcg ttacagaggt cacttagaca atatttcgga
     3481 taatatgtta atcggagcgg ttaatgcctt tacggacaaa acgaatgcag tactcgatac
     3541 cgaaacagga gattacattg ctgtaccagc gttagctcgc aaatacaaaa ctgctggaat
     3601 aggctcaatt gtggtggctg aagataatta tggagaaggc tcaagtcgcg aacatgctgc
     3661 tatggagcca cgtttcttga acgtaaaagc aattgtggtt aaatcttttg ctcgaataca
     3721 cgaaacaaac ttgaaaaagc aaggattact tgctcttaca tttgttaata aagatgatta
     3781 ttccaaaatt cgcgaaaatg atacaatcag tattttgggt gtaacgaaca tgaaaccagg
     3841 agtcccctta acaatggtgt tacatcatgt tgatggaaca gaagaatcat ttgatgtagc
     3901 acatacctat aacttagcac aaataaactg gtacaaatcg ggaagtgctc taaatgcgtt
     3961 aaacaaaatt taataataca aactagaatg gaaaaagaat atttggtata taaattgtca
     4021 gaaacaatta aaactaccac acgaattgat agagaattat ttacctccta taatgtaaaa
     4081 cgtggtttgc gtaacgaaga ccattcgggc gtattggttg gattaacgaa cattggcgat
     4141 gtggtgggct acgaaagact gcccgaagga ggattaaaag ctattccagg aaagttgttt
     4201 tatagaggaa tagatgttga ggatttagca aaaggaattc aaaaagacaa ccgtgtaggt
     4261 tttgaagaaa cagcattctt gttactttct ggctatttac ccgacaaaga agaattagat
     4321 actttttcta gtatcctgaa tgactcaatg gtgcttgaga aaaaaatgaa aatgaacatt
     4381 ttggaactcg aagggcaaaa tatcatgaat attttggctc gaagtgtgct cgaaatgtat
     4441 acgtttgatg aaaatccaga tgacatttct cgggataatc tcattcgcca atctatcgac
     4501 ctaatagcaa aatttccaac aattattgcc tatgcataca atatgatgcg tcatagtacg
     4561 cattgtcgat cgttgcatat tcgccaccct aaagacaagg tttctttagc cgaaaacttt
     4621 ttgtacatga tgcaaggtcc acgtagctat tcggatttgg atgtaaaaat tcttgactta
     4681 gccctcattt tgcacgccga acatggcggg ggtaataatt ccacctttac agtgcgtgtt
     4741 actagctcgt ctgaaacaga cacctactct tctatagcgg caggtattgg ttcactaaaa
     4801 ggacctcttc atggaggtgc taacttgcaa gtagtagata tgttggagca cctaaaagaa
     4861 gctatttccg attggacaga tgctgctgag ttagatgctt atttacaaaa aatgctaaat
     4921 aaagaagtgt acaacaaaac agggaaaatc tatggtatcg gtcatgctat ttataccatc
     4981 tctgacccaa gagctttatt gctaaaagaa atggcacgca aattagccga agagaaagga
     5041 ttacaaaaag agtttgcatt cttagaattg attgaaaata gagctattga aacatttatg
     5101 aatttcaagg gagaacgtat caataaacaa gtatgtgtaa atgttgactt ctattctgga
     5161 tttgtatacg atgctatcgg tttaccaaga gaagtataca cacctctatt tgctatgtcg
     5221 cgtatagcag gatggacggc tcatcgtata gaggagttga actttgctag caagcgtatc
     5281 attcgccctg catacaaaaa tgttggaggg aaaaaagaat ttattcctat tcaaaaacga
     5341 taataataaa aacagataat tatccataaa cgtactattc cgatgcaaaa aataactgta
     5401 caaaaccccg tagtagaatt agatggagat gaaatgacac gcattatttg gtcatttatc
     5461 aaagaacaac ttatacttcc ttatctgaac gtagatataa aatattacga tttgggtatg
     5521 gaaaaccgcg acgccaccaa tgatgaagta accgtagaag ctgctaacgc cataaaaaaa
     5581 tacaatgtag ggattaaatg tgctaccatc acgcccgacg aagcacgcgt tgtagaattt
     5641 ggtctaaaaa aaatgtggaa atctcctaat ggcacgctac gtaatattat tggtggaacc
     5701 gtatttcgcg aaccaattat ctgctccaac gtgccacgtt tagtacccaa ctggactcaa
     5761 ccaattgtta tcggacgtca tgcttttggc gaccaataca aggctaccga tgtagtaatt
     5821 aaaggtaaag gtaaattaac catgtcgttt actaacgaaa atggggaaac aacacaatgg
     5881 aatgtatatg attttgaagg ggatggagta gctatgtgta tgtacaatac agacgattct
     5941 atttatggat ttgcacgttc ttcttttcaa atggcattaa acaaaaaatg gccactgtat
     6001 ctgagcacta aaaacactat tttgaaagct tacgatggac gttttaaaga catttttcaa
     6061 gatgtatatg aaaaagaatt taaagctgat tttgaagcaa atggattggt gtatgaacac
     6121 cgattaatcg atgatatggt agcagctgca ctaaaatgga acggcggatt tgtttgggct
     6181 tgtaaaaact acgatggcga cgtgcaat
//
LOCUS       DAOSRP010000107         6060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_11944_length_6060_cov_6.882431, whole genome shotgun sequence.
ACCESSION   DAOSRP010000107 DAOSRP010000000
VERSION     DAOSRP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11944_length_6060_cov_6.882431"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1537
                     /locus_tag="PLJ82_07045"
     CDS             158..1537
                     /locus_tag="PLJ82_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOR41296.1"
                     /translation="MITKAYITELKHTGNGDIAKLIKSQRDVGTINNILESLGQLPAN
                     FESDFLYELLDHQHAQVRLNAVKNIGKLNGKSKTIKLHELYERETDTSVRREIISSIG
                     RQRKPENKIFLFETLHDDDPKIVCQAIRALLVFEKDIDVQEHLKPLINHTNEMVRNVI
                     YKEYFAKDNQKNGVLPHTETYDFLKNTVVNADVLEALKFVPDESVHLTFTSPPYYNAR
                     DYSIYPSYEAYLEFLENVFKETHRITKEGRFLIVNTSPIIIPRVSRSHSSKRYGIPFD
                     LHPLLVKNGWEFLDDIIWLKPEASVKNRIGGFMQHRKPLGYKPNCVTEYLMVYRKQTE
                     KLLDWNIRSYDYQTVEESKVADGYETTNVWKIDPTFDKVHSAIFPVELCKRVIQYYSY
                     KGDLIFDPFGGSGTVGKTAKSLNRSFFLTEQEPTYFEYMKAKAKPGALFKERETRFLT
                     LEQFKNIAK"
     gene            1534..2442
                     /locus_tag="PLJ82_07050"
     CDS             1534..2442
                     /locus_tag="PLJ82_07050"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="HOR41297.1"
                     /translation="MTLTDQVVKNIITRVIKSQDYRIEIVNLINAEFLQFAVEFFKKI
                     ANAKLNSEEITIDWYKKAFMDDSLSSDEIAINSGLNKKTISNMYGTATKTIVIEAANE
                     HFESLYNSIQSLVELEKEIDLTLTIKMKGVSVDLNVSESLIVINTLAVKRAALRGGLW
                     STAGKSAEKYLMLTLCKMYQVEEKYYDASHFVKDKGKKVDREIDFYLLNNGKEYLCEV
                     KLMGKGNPESADAIIARDTNVFVADTLSLQNKNQCEQLNINWIALRDENGFKRFKIAL
                     EKLGIPHVDYLGDLEIDLPKILDSLF"
     gene            complement(2762..3727)
                     /locus_tag="PLJ82_07055"
     CDS             complement(2762..3727)
                     /locus_tag="PLJ82_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOR41298.1"
                     /translation="MEKPYVVGIDIGGTNTVFGIVDARGSVLATNSIKTQKHTVLEDY
                     IQELYDELTKLIADNTHISKIKGIGIGAPNANYFNGTIEQAPNLPWKGIIPFADKIQK
                     KFGLPVAITNDANAAAIGEMIYGAARGMKNFIMITLGTGVGSGIVANGSLVYGSDGFA
                     GELGHTTMRRNNGRLCGCGLTGCLETYASATGVARSAREFLETQKDVPSRLRDIPADE
                     ITSKDVYEAAVAGDKLAQEVFAFTGQILGEAFADFIKFSSPEAIIMFGGLSKAGELLL
                     RPIRESMEKNVMPIFRNKVKLLFSELKEADAAVLGASALAWEIKD"
     gene            4117..5085
                     /locus_tag="PLJ82_07060"
     CDS             4117..5085
                     /locus_tag="PLJ82_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOR41299.1"
                     /translation="MTEQVIVLEHADPTIFCGVNNAHILVIKNLFPKVRIIARGNAIK
                     AMGDKDEIALFAEKITKIEQHITEYNNLSEEVIINIIKGETPLVCKPNNLILHGLNGK
                     PITARTENQQKLVELFDTHDMLFAEGPAGSGKTYTAIALAVRALKNKTAKKIILSRPA
                     VEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMIPAVKLKDYIENNIIQIAPLAFMRGR
                     TLNDAIIILDEAQNTSTQQIKMFLTRLGLHSKMIITGDITQIDLPSSQVSGMIHAMKI
                     LRHVEGVAHIHFTKQDIVRHKLVSRIIEAYEQHALEIKQQKEINTK"
     gene            5111..6058
                     /locus_tag="PLJ82_07065"
     CDS             5111..6058
                     /locus_tag="PLJ82_07065"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOR41300.1"
                     /translation="MNALTKTDFNFDKQKSVYHGKVRDVYNINDELLVMVVSDRISAF
                     DVVLPKGIPYKGQMLNQIAAKFLDATADIVPNWKLATPDPMVTVGVHCDSFPVEMIVR
                     GYLCGSAWRAYQKGVREICGVKIPDGMRENQRFAEPIITPTTKADQGFHDEDISKEEI
                     LKQGLVSAEDYALLEKYTMQLFLRGTEMALERGLILVDTKYEFGKKNGQIYLIDEIHT
                     PDSSRYFYASEYQARFEKGEQQKQLSKEFVREWLMENGFQGKEGQQVPEMTDEIVASI
                     SERYIELFENISGEKFIKAPTENILERIEKNVSKYLATR"
BASE COUNT         2141 a         1046 c         1109 g         1764 t
ORIGIN      
        1 gtttgacagt tgaatcgtcc gcaatccgcc actaggcata tgcgtaacgt tatgcccaat
       61 tttaaaaaaa agaaaacaaa ggtcatttga cggttgagaa ggatgatgaa aataggcaaa
      121 ttatgactat ttttgcaaca aaacaaaaaa gacaaagatg ataaccaaag cttacataac
      181 agaacttaaa cacactggaa atggcgacat tgctaaactt attaagagtc agcgagacgt
      241 cgggacaatt aataatatcc ttgaaagttt agggcaacta ccagctaatt ttgagagtga
      301 ttttttgtat gaacttcttg accaccaaca cgctcaagtt cgactaaatg ctgttaaaaa
      361 tataggaaaa ctaaacggca agtcaaaaac tataaaactt cacgaattat acgaaagaga
      421 gactgacaca agtgtcagac gagaaatcat ttcttcaatc gggagacagc gaaaacctga
      481 aaataaaatt ttcttatttg aaacattgca tgatgatgac cctaaaatag tttgtcaagc
      541 tatacgagca cttttggttt ttgaaaaaga tattgatgtt caagaacatt taaaaccatt
      601 aattaatcat acaaatgaaa tggttagaaa tgtgatttat aaggaatact ttgcaaaaga
      661 taatcaaaag aacggtgtgc ttccgcacac cgagacttat gattttctta agaatactgt
      721 cgtaaatgca gatgttttgg aagccttaaa gtttgttcca gatgaaagtg ttcatttaac
      781 ttttacttcg cctccttatt acaatgcaag agattattcg atatatccaa gctatgaggc
      841 atacttagaa tttttggaga atgtattcaa agaaacacat agaattacta aagaaggacg
      901 ttttttaata gtaaatactt cgccaatcat tattccacga gtaagccgtt cacattcaag
      961 taaacgttac ggaatacctt ttgacttgca cccgctttta gtgaaaaatg gatgggaatt
     1021 tttggatgat attatctggc ttaaacctga agcaagtgta aaaaacagaa ttggcggatt
     1081 tatgcaacac cgaaaaccgc ttggttataa acctaattgc gttactgaat atttaatggt
     1141 ttatagaaag caaaccgaaa aacttttaga ttggaatata agaagttatg attaccaaac
     1201 cgtagaggaa agcaaagtag cagatggtta cgaaactaca aatgtttgga aaattgaccc
     1261 aacttttgac aaagttcatt cagctatttt cccggttgag ttatgtaaaa gagttataca
     1321 atattattca tataagggtg acttaatatt tgacccattt ggtggtagtg gaacagtggg
     1381 caaaactgca aaatcattga ataggtcttt tttcttaaca gaacaagaac ctacatattt
     1441 tgaatacatg aaagcaaaag caaaaccagg agcgcttttt aaagaacgtg aaaccagatt
     1501 tctaacacta gaacaattta aaaatatagc aaaatgacac taacagacca agttgtaaaa
     1561 aatataatca cacgtgtaat caaatcacaa gattaccgta tcgaaattgt gaatttgata
     1621 aatgctgagt ttcttcaatt tgcggtcgaa tttttcaaaa aaattgcaaa tgcaaagtta
     1681 aactctgaag agattacgat tgattggtat aaaaaagcgt ttatggacga ttctttgtcg
     1741 tcggatgaaa tcgccataaa ttcagggctt aataaaaaga caatcagcaa catgtatggt
     1801 actgcaacaa agactattgt cattgaagca gcaaatgaac actttgaatc tctttataat
     1861 agtattcagt cgcttgtcga attggaaaag gaaattgact tgacattaac cataaaaatg
     1921 aaaggtgttt ctgttgattt gaatgttagt gaaagtctga ttgtcattaa tactttagcg
     1981 gttaaacgcg ctgcattaag aggtggctta tggagcacag caggaaaaag tgctgaaaaa
     2041 tatctaatgc ttactctatg taaaatgtat caagtagaag aaaaatatta tgatgctagc
     2101 cattttgtta aggataaagg aaagaaagtt gacagagaaa tagactttta tttgcttaat
     2161 aatgggaaag agtatttgtg tgaagttaaa ttaatgggaa aaggaaatcc tgaaagcgcc
     2221 gatgcaatta ttgcaagaga tacaaatgtt tttgttgccg acacactttc acttcaaaac
     2281 aagaatcaat gtgaacaact aaatataaat tggatagcat tacgtgatga aaatggtttc
     2341 aaacgattta aaatagctct tgaaaaatta ggtatacctc atgttgatta tttaggcgat
     2401 ttagaaatag acctacccaa aattttagat agtctatttt gaaagacata atttcatttt
     2461 gaaagacaaa aaaactgggc ataacacacg cctatgcgca ttggcggctg acgtgcattt
     2521 agcggcattt gctcccgcat tagctacgct gattttcaat tcactttgtc gcagtgcgac
     2581 tgtcttgtcc tgaaataggt ttacacaaaa aagtgtaaaa tggaaacaaa aaaacaaggt
     2641 attttaggat tacggagaaa gaaatccact aacaccaata cccaacaata ctctacaagt
     2701 ataaaaaata agcctaagca ttagcttagg cttatttgta aatagtgttg aaacgtgcgt
     2761 attaatcttt tatttcccaa gctaaggcac tggcacctaa tacggcagca tctgcttctt
     2821 tgagttcgga gaacaataat tttactttgt ttctaaaaat tggcattacg tttttctcca
     2881 tcgattcgcg tattggacgc aataataatt ctcctgcttt tgacaaaccg ccaaacataa
     2941 taatggcttc ggggctagag aatttaataa aatcggcaaa agcttcgcct aaaatctgcc
     3001 ctgtaaaggc aaatacttct tgtgctagtt tatctcctgc tacagcagct tcgtacacat
     3061 ctttggaagt aatttcatca gcgggaatat ctctcaatcg gcttggtacg tctttttgtg
     3121 tttctaaaaa ttcgcgagcc gaacgtgcca cacccgtagc cgaagcatag gtttctaggc
     3181 atcctgttag tccacaaccg cacaaacgtc cgttgttgcg acgcatagtg gtatgtccta
     3241 attcaccagc aaacccatcg ctaccataca caagcgagcc attggctact ataccactcc
     3301 ctactcctgt gcctaaggta atcataataa agtttttcat accacgagct gctccataaa
     3361 tcatttcacc tatagctgct gcattggcat cgttggtgat ggctacaggt aagccaaatt
     3421 tcttttgaat tttatcagca aacggaataa tacctttcca tggtaagttg ggggcttgtt
     3481 cgatagtacc attaaaataa tttgcattgg gagctccaat acctatgccc ttaattttag
     3541 aaatatgggt gttatcggca attaatttag ttaactcatc atacaattcc tgtatataat
     3601 cttctaacac agtgtgtttt tgtgttttaa tcgaattagt agccaataca cttccacgtg
     3661 catctacgat accaaaaact gtgtttgttc cgccaatatc aatacctact acgtaaggct
     3721 tttccataca tttattcttt ttaaggttaa tattcaagtg tttactattt gtttactata
     3781 aaaacaaaaa agcctcatca agcatacctt aacaaccgtg aaggttgata agactcatta
     3841 ctactaagta aataatgtct ccttttgcac acttactata gctaaagatg ggctttggtg
     3901 ctacaaaaat actatttttt tgataaatga tgctattggt agaaaatttt tatggtgtaa
     3961 aacagaaaga attcacatta cttgttttat atccctatct tttttctcta tgatagaaag
     4021 tttcctatcc taatattact taaaaaatta agtaatgata aatttgctgt aaaatataaa
     4081 agaaaatatg tacctttgca taccaaaatt ttaccaatga cagaacaagt aatcgtgttg
     4141 gaacatgctg accccactat cttttgtggt gtcaataatg ctcatatttt agtaataaaa
     4201 aatctgtttc caaaggtacg tattatagca cgaggaaatg ctattaaggc aatgggcgat
     4261 aaagacgaaa tagctctgtt tgccgaaaaa attacaaaaa tagaacagca tattaccgaa
     4321 tacaacaact tatcagaaga agttattatt aatatcatca aaggagaaac accccttgta
     4381 tgcaagccaa ataatttaat tttgcatggg ctgaatggaa aaccaataac agctcgcact
     4441 gaaaatcagc aaaagttggt agaattattt gatacacacg atatgctatt tgccgaagga
     4501 cctgccggat cgggaaaaac gtatacagcc attgctttag cagtgagagc attgaaaaat
     4561 aaaacagcaa aaaaaattat tttaagcaga ccagctgttg aagcgggcga aaagttgggt
     4621 ttcttgcctg gagatatgaa agaaaaaatt gacccttact tacaacctct gtacgatgcc
     4681 ttgcaagata tgattcctgc agtaaaatta aaggattaca tcgaaaacaa catcattcaa
     4741 atagctccgt tggcttttat gcgtggacgc acactcaacg atgccattat tattttggac
     4801 gaagctcaaa atacgagcac acaacaaatc aaaatgtttc ttacccgctt aggtttgcac
     4861 tctaaaatga ttatcactgg tgatattacg caaattgact taccctcatc gcaagtgtct
     4921 gggatgatac acgctatgaa aattttacgc cacgtagaag gtgtagctca tattcatttt
     4981 accaaacaag atattgtacg acacaagttg gtaagtcgca ttattgaggc atacgaacaa
     5041 catgctcttg aaataaaaca acaaaaagag ataaacacta aataaaaacc cactctaaac
     5101 cctaacgaca atgaatgcac taacaaaaac tgatttcaac ttcgacaagc aaaaaagcgt
     5161 gtatcatggc aaagtacgtg atgtgtataa cattaacgac gaattattgg taatggtagt
     5221 aagcgaccgt atttcggctt ttgatgtagt attgcccaaa ggtattcctt acaaaggaca
     5281 aatgctgaac caaattgctg ccaaattttt ggatgctact gctgatattg tgccaaactg
     5341 gaaattggct acacccgacc caatggtaac agtgggtgtg cattgcgatt cgtttcctgt
     5401 agaaatgatt gtgcgtggct acttgtgtgg cagtgcatgg agagcctacc aaaaaggtgt
     5461 acgtgagatt tgtggtgtaa aaatacctga tggcatgcgt gaaaatcaac gttttgctga
     5521 gccaattatt actcctacca caaaagcaga tcaaggtttt cacgacgaag atatatcgaa
     5581 agaagagatt ctaaaacaag gacttgtgtc ggctgaggac tatgctttgc tcgaaaaata
     5641 caccatgcaa ctatttctaa gaggtactga aatggcttta gagcgtggat taattttggt
     5701 agatacaaaa tatgagtttg gcaaaaaaaa tggtcaaatt tatttgattg acgaaattca
     5761 tacgcccgat tcttctcgct atttttatgc ctccgaatac caagcacgtt ttgaaaaagg
     5821 agaacaacaa aaacaattat cgaaagagtt tgtacgcgaa tggttgatgg aaaacggttt
     5881 tcaaggcaaa gaaggacaac aagtacctga aatgacagat gagattgtag ccagcatcag
     5941 cgagcgttat atcgagttat ttgaaaatat atctggcgaa aaatttatca aagctcccac
     6001 agagaatatc cttgaacgga ttgagaagaa tgtaagcaag tatttagcta ctagataatt
//
LOCUS       DAOSRP010000108         6028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_12017_length_6028_cov_5.482002, whole genome shotgun sequence.
ACCESSION   DAOSRP010000108 DAOSRP010000000
VERSION     DAOSRP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_6028_cov_5.482002"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /gene="asnB"
                     /locus_tag="PLJ82_07070"
     CDS             complement(<1..1441)
                     /gene="asnB"
                     /locus_tag="PLJ82_07070"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOR41301.1"
                     /translation="MCGFVGLFEIKEKESELRPQVLKMSKKIRHRGPDWSGIYCGEKA
                     VLSHERLSIVDPQSGSQPLYTKDKKVVLAVNGEIYNHQQIRERLSDQYEFLTRSDCEV
                     ILALYREKGINFLEDLSGIFAFALYDIEKDEYLIARDHIGIIPLYMGWDKNNQFYVAS
                     ELKALEGTCVRIEEFLPGHYLSSKDCKITPWYKRDWMKYDAVNNNSSSIDELREALEK
                     AVERQLMTDVPYGVLLSGGLDSSIISAVAKKYSTKRIESGSIQDAWWPQLHSFAVGLD
                     GSPDLIAARKVADYIGSVHHEIHFTIEEGLDAVSDVIYHLETYDVTTVRASTPMYLMA
                     RVIKSMGVKMVLSGEGADEIFGGYLYFHKAPNAKAFHEETVRKLSKLHSYDCLRANKS
                     LAAWGVEGRVPFLDKEFMDVAMRLNPTDKMAGNGKMEKWILRKAFESYLPEEVVWRQK
                     EQFSDGVGYSWIDNLKLIANERITDTMMKN"
     gene            1823..2470
                     /locus_tag="PLJ82_07075"
     CDS             1823..2470
                     /locus_tag="PLJ82_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOR41302.1"
                     /translation="MIWNNIDDLVNGKDDVFSDVTFSCTQYPKNELVFRQNDICDSLY
                     LLSKGSVKTEMITENGNLLGIEIIHAPRPLAPAFLFSEDNRFPVDVTSITDIEIIKIP
                     KKEVVRLMSLQPDFMHQFISHNSNRTQFLTHRLQLLTIKTIKGKIAYFLLEQMQEYGN
                     PFMLERNQTELADFFNIPRPSLARSLSELVQENAIRIERKKITILDENKLKELLV"
     gene            complement(2671..3627)
                     /locus_tag="PLJ82_07080"
     CDS             complement(2671..3627)
                     /locus_tag="PLJ82_07080"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR41303.1"
                     /translation="MIIGENIKQAALIIREGGLVAFPTETVYGLGANALNPMAVAKIF
                     EAKQRPSFDPLIVHIHHFDEIYALFSSPISHWVQKLAEAFWPGPLTIVHKKSTSVPHI
                     VTSGLETVAVRMPSHPTAIALLKASECAIAAPSANRFGQISPTEASHVTKQLQDIDYV
                     LLGEKVTLGIESTVVSVADEGCFILRPGVITAEDLEKVVPLCSHNPNETIGSLSSPGL
                     LHSHYAPTKPMYLLDNTMDILPPHSGIIVHTNKKNWNTAQALFITSQNGDKKEIASNL
                     FAAFHTMEDNKAIQQIFIEPVEENGIGIAIMDRIKKAVFKYK"
     gene            complement(3632..4108)
                     /locus_tag="PLJ82_07085"
     CDS             complement(3632..4108)
                     /locus_tag="PLJ82_07085"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOR41304.1"
                     /translation="MKVIILMGSKADLDWGTQIKIVLDRFEIPSVIRIASAHKVPLKC
                     YALIKEYEKENVIFITIAGMSNALSGFSDAQTYCPVIACPPYSDKFGGTDIFSSVRMP
                     SGVAPLTVLSPENAALAAAKIFGLSNPSLREKIAAFQEKKREELEEADALLQKSQA"
     gene            complement(4247..>6028)
                     /locus_tag="PLJ82_07090"
     CDS             complement(4247..>6028)
                     /locus_tag="PLJ82_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HOR41305.1"
                     /translation="DIADAAVGGKDKLEFEVKYDVPGKGEYTEAIVHRVTNGISANYT
                     EAYMRRRDPNTMAIGDNLPTDKKRFKDKYGYEFSNLRKETFDWLKTQELAIFFYFAGN
                     NGIGVPGIAIVPANAGFFAMGLAMLQHIVPINEMEDSFAIESVLYVAPVFRHTHFEGK
                     QVVVHNRLEQLHEIFAYNLYPGPSAKKGMYAALLDKGEKEGWVTTHCSSVQAISPYDN
                     TTAFMHEGASGGGKSEMMQHLARELNGQVMIGKNVVTGETRAISLPIFCSFNPITDDM
                     ALCHPSFQKTPGKLSISDAENGWFIRVDSVCDYGDDPHLEKQTIKVEKPLLFLNIQSH
                     PGGTALIWNHTEDEPGKPCPNPRVILPRETVPGSIAKPVAIDIRSFGVRTPPCSSENP
                     NYGIFGLFHILPPALAWLWRLVSPRGHANPSIIGGDGMGSEGVGSYWPFATGKRVTHA
                     NLLLGQIMQTPKMRYTLVPNQYIGVWKVGFKPQLLMREYLTRRGNGNLKPEQYQPARC
                     SLLGYELNFLSIEGAKIPSRFLQVYKQPEVGFEGYDKGADILREFFKKELPQYLTPEL
                     HPVGRKIIEACLADASVEDYNDIIPFI"
BASE COUNT         1838 a         1109 c          994 g         2087 t
ORIGIN      
        1 catttttcat catggtatcg gttatgcgtt cattggcaat cagttttaag ttgtctatcc
       61 agctatatcc tacaccatca gaaaattgtt ctttttgacg ccacacaacc tcttcgggta
      121 aatacgattc aaaggcttta cgcaatatcc atttttccat ttttccatta ccagccattt
      181 tatctgtagg atttaagcgc atagcgacat ccataaattc tttgtctaaa aatggaacgc
      241 gaccttctac accccaagct gctaaggatt tattcgctct caaacagtcg taagaatgca
      301 gtttggataa ttttcgcact gtttcttcat gaaaagcttt agcgtttgga gctttatgga
      361 aatacaaata tccgccaaaa atttcgtcag caccctcgcc agataatacc atttttactc
      421 ccattgattt gattacacgt gccatcagat acataggggt agaagcccta accgtggtta
      481 catcatatgt ttcgagatga taaataacgt cacttacagc atctaaacct tcttcgatgg
      541 taaagtgtat ttcgtggtgt actgaaccaa tgtagtcagc tactttacga gcagcaataa
      601 ggtcgggcga accatctaat cctacggcaa atgaatgtag ttgaggccac caagcatctt
      661 gaatactgcc agactcaata cgtttggttg aatatttttt tgctactgct gaaataatag
      721 atgaatctaa tcctccagac aataatacgc catatggtac atctgtcatt aattgtcttt
      781 caactgcttt ttctagagcc tctcgcaatt catcaatgga agaagagttg ttgtttaccg
      841 catcatattt catccaatcc cgtttgtacc atggcgtgat tttgcaatct ttactactta
      901 aatagtgccc aggtaaaaat tcctcaatac gcacacaggt tccttctaag gctttaagtt
      961 cagatgcaac ataaaattga ttgtttttat cccaacccat gtacaatggt ataattccaa
     1021 tatgatcgcg tgcaattaaa tattcatctt tctcaatatc atatagtgca aatgcaaata
     1081 taccactcaa gtcttctaaa aaattgatgc ctttttcgcg atataatgct aaaattactt
     1141 cgcaatcaga acgtgttaga aattcatact gatcacttaa cctttctcta atttgttgat
     1201 ggttataaat ttctccattt acagccaaaa caaccttttt atcttttgta tacaacggtt
     1261 gactcccaga ttgggggtcg acgatagata agcgttcgtg tgataaaact gctttttcac
     1321 cgcaatatat tccagaccaa tctggtcccc gatgtctaat ctttttagac atctttaata
     1381 cctgaggacg taactcgctc tctttttctt ttatttcaaa taatccaaca aatccgcaca
     1441 tatatcatca ttttaatgtg aatagaaatc tattttttgc aaaattaaag aatatatttt
     1501 aaaatagtat cattttgttc gattttattg atataaatat ctataatgct attaaatttt
     1561 tataaaaaat acagaaaatc catctctaat ttacttatta aaataaatag tattcattct
     1621 tttatgattt agaagttctt taattggaag taatactatt tcttgttgta aaaatactac
     1681 tttgtacgtg ctattttgca ttatttcttt gatttttttt tatttagctt tgatttttat
     1741 agtaatattt tttcatttcg tgtaatttta tgtacttttg atatcaacat aatggcaaca
     1801 aactataaag aaaacagcga atatgatttg gaataacata gatgatttag ttaatgggaa
     1861 agatgatgtt ttttctgatg ttacattttc atgtactcaa taccctaaaa atgaattagt
     1921 ttttaggcag aacgatattt gtgattcctt atacttattg tcaaaaggaa gtgtaaaaac
     1981 agaaatgata acggaaaatg gcaatttatt gggaattgaa attattcatg ccccgcgacc
     2041 attagctcca gcgtttttat tttcagaaga taatcgattt cctgtagatg ttacgagtat
     2101 tactgatatt gaaataatta aaatccccaa aaaagaagtt gtacgcttaa tgtcattaca
     2161 acccgatttt atgcaccaat tcattagtca taattcaaat cgtacacaat ttcttacaca
     2221 tcgattacaa ttacttacca tcaaaacaat aaaagggaag attgcttatt ttcttttaga
     2281 gcaaatgcaa gagtatggaa acccttttat gttagaacga aatcaaacag agttagccga
     2341 tttttttaat attcctcgac cgtcgttagc tcgaagttta tctgaattgg ttcaagaaaa
     2401 tgctatacgt atagagagaa agaaaatcac tattttagat gaaaataaac taaaggaatt
     2461 actggtttaa tgtatatttc aaggttattt cggtaaccaa caaatttggt taatcttgtt
     2521 tatacaaatg agggtaaatc cttactctta tccaacgaat aaagtagcct gtctgtatga
     2581 aaatatcaga tggaatttta gatgatacga ttaataaaaa gtttacactt taagaatgta
     2641 tatgtagcat aatagcaact ggatcgttgt ttatttgtat ttgaatacag cttttttaat
     2701 tctatccata atggcaatac ctatgccatt ttcttctact ggttcaataa aaatttgttg
     2761 tatcgcttta ttgtcttcca ttgtatgaaa tgctgcaaat aaattagatg ctatttcttt
     2821 tttatctcca ttttgtgatg ttataaagag tgcttgagcc gtattccagt tttttttgtt
     2881 agtatgtaca ataatacctg aatgcggggg caaaatgtcc attgtattat caagtaaata
     2941 cataggtttg gttggagcat aatgactatg tagcaatcct ggagaggaaa gagaaccgat
     3001 tgtttcgttt ggattatgag aacaaagtgg taccactttt tctaaatctt cagcagtaat
     3061 tacacccggt cgtagaataa agcatccttc gtctgctaca gatacaaccg tagattctat
     3121 acccagtgtt actttttcgc caagcaatac ataatctata tcttgtaatt gctttgttac
     3181 atgagatgct tctgttggac ttatttgacc aaatctattc gcactagggg ctgcaatagc
     3241 acactcactt gctttaagta aagcaattgc tgtagggtga gagggcatcc gtacggctac
     3301 tgtttctagt ccagaagtaa caatatgtgg aacactagtt gattttttgt gtacaattgt
     3361 tagcggtccg ggccaaaaag cctccgctaa cttttgtacc caatggctaa taggagatga
     3421 aaacaaggca taaatttcat cgaaatggtg aatatgcaca atgagagggt caaacgaagg
     3481 tcgttgtttg gcttcaaata tttttgctac tgccataggg ttgagtgcat tagcccccaa
     3541 tccatatact gtttcagtag gaaatgcaac taaccctcct tcgcgtatta tcagtgccgc
     3601 ttgtttaata ttttctccaa taatcatttt tttaggcttg acttttttgc aaaagtgcat
     3661 ctgcttcttc taattcttca cgtttttttt cttgaaaagc tgctatcttt tcacgcaatg
     3721 atggatttga taatccaaat attttggctg ctgctaacgc tgcattttct ggtgataaaa
     3781 cagtcagagg agcaacaccc gaaggcatac gaacactaga gaaaatatca gtaccaccaa
     3841 atttatcact atatggtggg catgctatta caggacaata ggtttgtgca tcgctaaatc
     3901 cactcaacgc attgctcatg cctgcaatag taataaagat tacattttct ttttcatatt
     3961 ctttaattaa agcatagcat ttaagtggta ctttatgtgc agatgctata cggattacag
     4021 atggaatttc aaatctatct aatactattt taatttgtgt tccccagtct aaatcggctt
     4081 ttgagcccat taaaataatt actttcatgt cgttatttga ttgtgttatg ttacgtttaa
     4141 aattattttt cttatataaa atcgcctatg cgttgaaata atgttttgtt tttcaaactc
     4201 atttcaggca taggcgatat gttatccata gatagatatt gctctattaa ataaacggaa
     4261 taatatcgtt gtagtcttct actgatgcat cagccaagca agcttcaata atttttcttc
     4321 ctacaggatg taattctgga gttaagtatt gaggtaattc ttttttaaag aattctctca
     4381 aaatatctgc tcctttatca tatccttcga atccaacctc aggttgtttg tatacttgca
     4441 agaaacgaga tggaattttt gctccttcaa tagaaaggaa atttaattca tatcctaaca
     4501 gagaacaacg agctggttga tattgctctg gttttaagtt tccgtttcct cttctggtca
     4561 agtattctct cattaataat tgaggtttga atcctacttt ccatacacca atgtattgat
     4621 tcggtaccaa tgtataacgc atttttggag tctgcattat ttgccctaac aaaagattag
     4681 catgtgttac tctcttacca gtggcaaagg gccaatatga acctacacct tcagaaccca
     4741 ttccatctcc acctataata cttgggttag catgtccacg tggagaaact aatctccata
     4801 accatgctaa tgcgggtgga agtatatgga ataatccaaa tataccgtag ttaggatttt
     4861 cgctagaaca tggtggagta cgtacaccaa aactacgaat atcaatagct actggtttgg
     4921 caatgctacc aggcacagtt tcgcgaggta aaataacacg aggatttgga caaggtttac
     4981 ccggttcatc ttctgtgtga ttccaaataa gagcggtacc tcctgggtgt gattgaatat
     5041 ttaaaaataa tagaggtttt tctactttaa tagtttgttt ttccaagtga ggatcatctc
     5101 catagtcgca tacactatct acacgaataa accagccatt ttcagcatcg ctaattgata
     5161 atttaccagg ggttttttgg aaagatgggt gacacaatgc catgtcatcg gtaattgggt
     5221 taaacgaaca gaatattggt aaactgatag cacgagtttc tcctgttacg acgttttttc
     5281 caatcattac ttgtccattc aattctctag ctaaatgctg catcatctca ctttttccac
     5341 caccacttgc cccttcgtgc ataaatgcag tagtattatc gtatgggcta attgcttgta
     5401 cacttgaaca gtgtgtagtt acccagcctt ctttttctcc tttgtctaat agagcggcat
     5461 acattccttt tttagcacta ggacctggat aaaggttata agcaaaaatt tcatgtaatt
     5521 gctctaaacg gttatgaaca actacttgtt ttccttcaaa atgggtatgt ctaaaaactg
     5581 gagctacata tagaacagac tcaattgcga atgagtcttc catttcatta attggtacaa
     5641 tatgttgtaa cattgccaac cccatagcaa agaaaccagc atttgcaggc acgatagcta
     5701 tacctggtac gccaatgcca ttatttcctg caaaataaaa gaaaatagcc aattcttgtg
     5761 tttttagcca atcaaaagtt tcttttctta aattagaaaa ttcatatccg tatttgtctt
     5821 taaaacgttt tttgtctgtt ggtaaattat cacctatagc cattgtgtta gggtcgcgac
     5881 gacgcatata ggcttcagtg taattggcag aaataccatt tgttacacga tgaacaatag
     5941 cttcagtgta ttcacctttg cctggcacgt cgtattttac ttcaaattct agcttgtctt
     6001 ttccacctac agcagcatct gctatgtc
//
LOCUS       DAOSRP010000109         6008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_12056_length_6008_cov_5.953805, whole genome shotgun sequence.
ACCESSION   DAOSRP010000109 DAOSRP010000000
VERSION     DAOSRP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12056_length_6008_cov_5.953805"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="PLJ82_07095"
     CDS             <1..1034
                     /locus_tag="PLJ82_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008663635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOR41306.1"
                     /translation="DAMTDNPLESKTITLSCGKLTTGVSVKPWTGIFMLRNSSSPETY
                     FQAAFRVQTPWVIKNPDSKSPNKEEILKEECYVFDFAPNRALRQIADYACRLNVNESN
                     PEKNVEDFINFLPVLAYDGSSMKQVDAAGILDIAMSGTTATLLARRWESALLVNVDNN
                     TLARLMANEEAMEALMNIEGFRNLNQDIETIINKSESVKKAKKEANDRELTKKEKKEL
                     SDEEKEYKSLRKQIQEKLIKFATRVPVFMYLTDYRERSLKDVITQLEPGLFKKVTGLS
                     VKDFELLVSLGVFNSALMNDAVYKFKRYEDASLEYIGINKHQGQDVGLYDTVLSRKDY
                     EESFVNEPE"
     gene            1372..2409
                     /locus_tag="PLJ82_07100"
     CDS             1372..2409
                     /locus_tag="PLJ82_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOR41307.1"
                     /translation="MPETRQIEILNFIRNKANCSSGEIHKEFATSISYATVKRILSQL
                     LNEKFIVSEGKGKGTRYFISPSYELLYSIDLDNYYEKEIDERQIKEEFNLEIFELLEK
                     VAIFSKPELKKLSELQQKYTENVSHLSEFEYRKELERLAIDLSWKSSQIEGNTYSLLE
                     TERLLKDKETAAGKTKEEAIMLLNHKDAIDFIVENPDYLTPLRISKIEDIHSLLIKEL
                     AVDKNLRKRRVGISGTNYRPLDNEFKIREALESACKLVNMKENIFEKALIILVLISYI
                     QPFMDGNKRTARIVSNAILMNYKHCPISFRTIDSVDYKKAMLLFYEQNNISQFKEIFI
                     NQFEFAVKTYF"
     gene            complement(2561..3055)
                     /locus_tag="PLJ82_07105"
     CDS             complement(2561..3055)
                     /locus_tag="PLJ82_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOR41308.1"
                     /translation="MNNITTTIIEKMIQYFGTDVRRINHALKVYGFARCIAQKEKLTE
                     NELFIVDIVAILHDIGIIEAEKKYNSSNGKYQEIEGPAIAKNMLSDQHIDTTTIDRIC
                     FIIGNHHSYQKIDNMDFQVIVEADFLVNIYEDEMEKHSIENIRNKYFKTQTGIAIIQS
                     MYAD"
     gene            3528..4139
                     /locus_tag="PLJ82_07110"
     CDS             3528..4139
                     /locus_tag="PLJ82_07110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR41309.1"
                     /translation="MEDTFAHKALTWDNPRKISMTNKFVKEIEKLCSFSSSLKILEIG
                     TGTGLVGLQLVSKASEMLLIDTSPSMLQILEKKLQDNPYPHVKCLHATIETINATDFD
                     LVISFMTWHHIADLSSAFTSVYTLLKKGGKLLIGDLLTEDGSFHGDEFVPHLGFDMQM
                     LRTIAENHGFKVLQVYPYAEVEKQVQNGDINRYGQFVLFAEKI"
     gene            complement(4218..5048)
                     /locus_tag="PLJ82_07115"
     CDS             complement(4218..5048)
                     /locus_tag="PLJ82_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOR41310.1"
                     /translation="MNEIQRRKNFYEYKSIEPEKFSHIVHTIYASLHQGFILSEWIDG
                     DIISRFDKEIIRDIFKTHIEIEKKGLFECDLSKNNLLIDKDKQIIFFDFGYMYPYNPL
                     IHYNSDGKQLPIFHLCERLESRSLMQYLMDIENDSSLMIETFENTKRLALEAYSEKLI
                     WLEKNNADTDVIQWQKNWINQWEYSLKSPANLLETYELESFRSYVLDVHDDIGGKSCT
                     PMTIKKLDKILEQIKHNYPTLKIRNGLFWGDEKLNNSSLYDKYTKLKEQACRYQLHET
                     "
     gene            complement(5150..5311)
                     /locus_tag="PLJ82_07120"
     CDS             complement(5150..5311)
                     /locus_tag="PLJ82_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41311.1"
                     /translation="MNIVDQRRTYQLIFLQSKQEIEVGNKDHLPLPIDELVKIVPILV
                     VSNKYFLQD"
     gene            complement(5311..>6008)
                     /locus_tag="PLJ82_07125"
     CDS             complement(5311..>6008)
                     /locus_tag="PLJ82_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOR41312.1"
                     /translation="SGTLRVVIEKNTMSYVVVNDTNVMGLQYKMVEQLAKDWGVTVQY
                     IDASDLGSAINMLNNNEVDIVVWHIPIHVEMEDKIAFTVPVFTSRQVLVQRKNTPHTL
                     IRNQLDLARKTIHIPKDSPYKQRIQNLAKEIGDSIFIVEINEISIDSLFYKVANAEID
                     FTVCEKVVASIEKKKYPTLDTETAIGFKQNYAWAIRQNSSELQDSVNNWLNYFLETKE
                     YNRIYQKYTHSRK"
BASE COUNT         1934 a          983 c         1062 g         2029 t
ORIGIN      
        1 tagatgcaat gaccgataat ccattggaat caaaaaccat aacgctttct tgtggcaaac
       61 ttaccacagg cgtttcggta aaaccgtgga caggaatttt tatgttgcgt aattcctcca
      121 gtcctgaaac ttattttcaa gcggctttcc gtgtgcaaac gccttgggtc atcaaaaacc
      181 cggacagtaa atctccgaac aaagaagaaa ttctaaaaga agaatgctat gtgtttgact
      241 ttgccccaaa cagagcattg cgacaaattg ctgattatgc ttgtcgtctg aacgtcaatg
      301 aatcaaaccc tgaaaagaac gtagaagatt ttatcaactt cctgccagta ttggcttatg
      361 acggcagttc gatgaaacaa gttgatgcag caggaatttt ggacattgct atgagtggca
      421 caacagccac acttttagcc agacgttggg aaagtgcttt gcttgtcaat gtggacaaca
      481 acacgcttgc ccgattgatg gcaaatgaag aagcaatgga agctttgatg aacatagaag
      541 gcttcaggaa tttgaatcaa gacattgaaa cgattatcaa taaatcggaa tccgttaaaa
      601 aagctaagaa agaagccaac gaccgagaac tgaccaagaa agagaaaaag gaactttcag
      661 acgaagaaaa agaatataag agcctgagaa agcaaatcca agaaaagctc atcaagttcg
      721 ctactcgtgt acctgtgttt atgtacctga cagactaccg tgagagaagt ctaaaagacg
      781 taatcacaca attagagccg ggacttttca aaaaagtaac agggctttcg gtgaaagact
      841 ttgaattatt ggtaagtctt ggcgtgttca actctgcctt gatgaatgat gccgtttaca
      901 aattcaaacg gtatgaagac gcaagtttag agtatattgg aatcaacaag catcaaggac
      961 aagacgttgg actttacgac actgtattga gcagaaaaga ttatgaagaa agttttgtaa
     1021 acgaacccga atagccaaat gccaacactc aacagccaca caattactgt cttgtcctga
     1081 aataggttta cacaaaaaag tgtaaaatgg aaacaaaaaa caaggcattt taggattact
     1141 ccccatgccc ctcgtttgta acagcagtaa tacgctaacc ccaagcatgg tagtcaaaaa
     1201 tcccgattat atgttgaaag ttcgttaaaa cccattacat ttgagcaaaa attaagctac
     1261 atggaggttt ttagacagaa tccctttatg gctcactata aaaaaaataa agctcgtttg
     1321 tggtgaattt tgagccgtat ttcgtatatt tgtgagctaa aacacaagat tatgccagaa
     1381 acgagacaaa tagaaatttt aaatttcatt cgaaacaaag caaattgttc ttcgggcgaa
     1441 attcacaaag agtttgcaac ttcgattagc tatgccacag ttaaacgaat tctatctcaa
     1501 ctgctaaacg agaagttcat agtgtcggaa ggaaaaggga aaggaacaag atattttatt
     1561 tcaccgtctt acgaattgtt gtattcgatt gacttagaca attattacga aaaagaaatt
     1621 gacgaaagac aaattaaaga agagttcaat ttagaaattt ttgaattgtt agaaaaagta
     1681 gctattttta gcaaacctga gctgaagaaa ctttcggagc tacaacaaaa atacactgag
     1741 aatgtttcgc atttatcaga atttgaatac agaaaagaac ttgaaaggct agccattgat
     1801 ttaagttgga aatcgtcaca aattgaagga aatacatact ctctgctaga aactgaaaga
     1861 ctgcttaagg acaaagaaac ggctgctgga aaaacgaaag aagaagcaat tatgcttctg
     1921 aatcacaaag atgccataga ttttatagtc gagaatcctg attatttaac ccccttgaga
     1981 atctccaaaa ttgaagatat tcatagcctt ttgataaaag aattggctgt tgacaaaaat
     2041 ctgagaaagc gtagggtggg tatttccgga actaactatc gacctcttga caatgaattt
     2101 aaaatacgag aggctttgga atcagcttgc aagttggtga atatgaaaga aaacatattt
     2161 gaaaaagcat tgattatatt ggtgttgatt tcttacattc aaccatttat ggacggaaac
     2221 aagagaacag caaggattgt aagcaacgca atattgatga attataaaca ttgtcccatt
     2281 tcatttagaa cgattgattc tgtagattac aaaaaagcga tgttactctt ttacgaacag
     2341 aacaatattt cgcaatttaa agagatattt atcaatcagt ttgaatttgc ggttaagact
     2401 tatttttagg atgtgacacc attttctcca agcagagaat atggttgaaa ttggctcgga
     2461 tcaatacaga taatgattca ggtatattac aatttagtaa ataaaaagtg ctattattct
     2521 ttttctttta acgttcgtat ttgcgtgaat catatttgtt tcaatcagcg tacatacttt
     2581 gtattattgc tatacctgtt tgagttttaa agtatttatt ccgaatattc tctattgaat
     2641 gtttttccat ttcatcttca tatatattta ctaaaaaatc tgcttcaaca ataacctgaa
     2701 aatccatatt gtctattttc tgataggaat gatgattgcc aataatgaaa caaattctat
     2761 caatagttgt agtatctata tgttggtctg acaacatatt tttagcgatg gcaggtcctt
     2821 ctatttcttg gtatttgccg ttactggaat tatatttttt ttcagcttca atgataccta
     2881 tatcgtgtag tatagccact atatccacaa tgaatagttc attttctgtt agtttttctt
     2941 tttgtgcaat acacctggca aagccatata ctttaagggc atgatttatt cttcgaacgt
     3001 ctgttccaaa atattgaatc attttttcga taatagtagt agttatatta ttcatatttg
     3061 gtatgttaac gattgttgtt acagtatgtt gtgtgtgtct actaaaaatg gtttttctgt
     3121 atctgtttgc ttatgatggt tcgatgataa gcctttgttt cgcttaatta ttgctcaaaa
     3181 gtaagatttt tttaaggata gtacaaaata gagggaaaga attttttagt tagagtgttg
     3241 cacttataca tatagcattg cttagtctgc attctgtcgt tgttttacat ggtatttgta
     3301 aaagactagg ataagttaaa aatataaaaa agataatcga cagtgaacat agccgttgtt
     3361 attgagcaaa aaatctaatt aaattcattt tcttttgatt atttcgtatt ttatagcgaa
     3421 atttgtgaag gatgataatg caggtgtgtc ttatttcaaa catggtcttt taaagctcta
     3481 tcctccatta ttttatgctt acacaggaca atactatacc taattttatg gaagatacat
     3541 ttgcccataa ggctttaact tgggataatc cccgaaaaat cagtatgacc aacaaatttg
     3601 tgaaggaaat agaaaaattg tgttctttct cttcttccct caaaatactt gaaattggaa
     3661 caggtacggg tttggttggt ttgcagttag tatccaaagc ttcagaaatg ttgcttatag
     3721 atacatcgcc atcaatgcta caaattctag aaaaaaaatt acaagacaat ccatatcctc
     3781 acgttaagtg tctacatgct actatcgaaa cgattaatgc tacagatttt gatttggtta
     3841 tttcttttat gacttggcat catatagcag atttgtctag tgcatttact tctgtgtata
     3901 cacttttaaa aaaaggaggg aaactcctta taggcgattt gctcacagaa gatgggagtt
     3961 ttcatggtga tgaatttgtt cctcatttag ggtttgatat gcaaatgttg cgtactattg
     4021 ccgaaaatca tggatttaag gtgttacagg tatatccgta tgccgaggtc gaaaaacaag
     4081 tacaaaacgg ggatatcaat cgttacggtc aatttgtgtt atttgctgaa aagatatagg
     4141 catcaatagt cgtttagata ccaacttgtt gtccgttgta gctggaaaca gggcatcagg
     4201 aagtagatct tatgtgatta tgtttcatgt agttgatatc tacatgcctg ctcttttaat
     4261 ttcgtatatt tgtcatataa tgaagaattg tttagttttt catctcccca aaacagccca
     4321 tttcttattt ttaatgtagg ataattatgt ttaatttgtt ccagtatttt atctaatttt
     4381 ttaattgtca ttggtgtaca actcttgccg ccaatatcat catgtacatc taatacataa
     4441 gaacgaaatg attccaattc gtacgtttcg agtaagttag ctggtgattt caaactatat
     4501 tcccactgat taatccaatt tttttgccat tgaatcacat ctgtatcggc attgtttttt
     4561 tctaaccaaa ttaatttctc gctatacgct tctagtgcta gtcttttagt gttttcaaag
     4621 gtttcaatca tcaggctcga atcattttct atatccatta aatattgcat taatgacctg
     4681 ctttccaatc gttcgcataa atggaatata ggtagttgtt ttccgtcaga gttgtaatgt
     4741 ataagtggat tatacggata catatagcca aaatcaaaaa aaataatctg tttatcttta
     4801 tcaattaaca gattattttt agacaaatcg cattcaaaca gccccttttt ttctatttca
     4861 atatgagttt taaaaatatc acgtatgatt tctttatcaa aacgagaaat tatatcccca
     4921 tcaatccatt cagaaagaat aaacccttga tgtaaacttg cataaattgt atgtactatg
     4981 tgactaaatt tttctggttc aatggattta tattcgtaga aattttttct tcgttgtatt
     5041 tcattcaaaa atgaggtttg tccatcagta tttttgacta agatatgttc ccgtttcttc
     5101 tttaaattat aacaatgatt gccaacacgg attttataca caatggctgt taatcctgca
     5161 gaaaatactt gttcgacaca actagaatgg gcacaatttt tactagctca tctattggta
     5221 gagggagatg gtctttgttg ccaacctcaa tttcttgttt actttgcaaa aaaattagtt
     5281 gatatgttcg gcgttggtca actatattca ttattttcta ctatgtgtat atttttggta
     5341 aatacggttg tattcttttg tctctaaaaa atagtttagc caattattta cactatcttg
     5401 tagctctgat gagttttgac gtatagccca tgcataattt tgcttaaatc cgatagctgt
     5461 ttcggtatcc agtgtggggt attttttttt ctcaatagat gcaactactt tttcgcatac
     5521 agtaaaatca atttccgcat tggctacttt ataaaacagc gaatcaatgc ttatttcatt
     5581 tatttctact ataaaaatgc tgtctccaat ttcttttgcc aaattttgaa tacgttgttt
     5641 atagggggaa tctttgggga tgtgtatggt ttttcttgct aagtctaatt ggttacgaat
     5701 aagtgtatgt ggcgtgtttt ttcgctgaac caatacttgt ctgcttgtaa aaacaggtac
     5761 tgtaaatgca attttatctt ccatttctac atgtattgga atatgccata ctacgatatc
     5821 tacttcgttg ttattgagca tattaatagc cgaacctaag tcagaggcgt ctatgtattg
     5881 cacagttacc ccccaatctt tggctaattg ttctaccatt ttgtattgca aacccattac
     5941 gttggtatcg tttaccacca catagctcat tgtgtttttt tctatcacta ccctcagggt
     6001 gcctgatg
//
LOCUS       DAOSRP010000110         5781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_12657_length_5781_cov_5.330597, whole genome shotgun sequence.
ACCESSION   DAOSRP010000110 DAOSRP010000000
VERSION     DAOSRP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12657_length_5781_cov_5.330597"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLJ82_07130"
     CDS             complement(<1..896)
                     /locus_tag="PLJ82_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOR41313.1"
                     /translation="MNNTLLLDVQSKDISIALMENNTLVEFSKEPRDMSFSVGDIFLA
                     KVKKIMPGLNATFVDVGYDKDAFLHYLDLGSNFHSIAQFTKTLVNDRKKIPSMQKNSG
                     LPDLEKDGFIADILKSGQEILVQITKEPISTKGPRLTAEISIAGRSLVLLPFSNKISI
                     SQKIKSEEERNRLRQLLVSIKPKNFGVIIRTVAEGKKVAELDAELKILEKRWEEAIEN
                     IQKAKQFPSLILKEMSRAETILRDSFNPNFQHIYVNEKTIFEEIQAYVGLIAPEKNDI
                     VKLYTDDIPIFDAFNITKQIKSP"
     gene            complement(1115..1399)
                     /locus_tag="PLJ82_07135"
     CDS             complement(1115..1399)
                     /locus_tag="PLJ82_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR41314.1"
                     /translation="MTKAEIVNEIANKSGIDKVTVLRVVESFMDEVKDSLASNENVYL
                     RGFGSFVVKQRAEKVARNISRNTTITIPAHSIPSFKPAKTFVEAVKNKII"
     gene            1562..2620
                     /gene="mutY"
                     /locus_tag="PLJ82_07140"
     CDS             1562..2620
                     /gene="mutY"
                     /locus_tag="PLJ82_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOR41315.1"
                     /translation="MTFSEIIIDWYEVNKRNLPWRNTKDAYTIWVSEVILQQTRVVQG
                     LAYFIRFIERFPTVIALAQADEEEVLKYWQGLGYYSRARNMHKAAQQVVELYDGIFPT
                     HYPHLLTLQGIGEYTAAAISSFAANEAHAVVDGNVYRVLSRVFGIFLAINSNEGKREF
                     TKLAQSLLPTHQASTYNQAIMEFGALQCVPARPLCSTCPLQVMCYAYTHQHVSSLPKK
                     EKKTTTKTRYFFYLDIRNGEYTYLQKRTQNDIWHNLFEYPLVECDKETSIENVINSED
                     FQEIVEKCEKTSIHSISSEIKHVLSHQIIRTIFIRIHIETDNSLLQSLYKINSRDIED
                     YPISRLMHKYIEKITNET"
     gene            complement(2625..2918)
                     /locus_tag="PLJ82_07145"
     CDS             complement(2625..2918)
                     /locus_tag="PLJ82_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41316.1"
                     /translation="MKSVFIVFNQANTERVEYMLDRLSIRGFTFWENVQGVGSMNGEP
                     RRGTHTWPEMNSAVMTVIPDEKVDDLLIAVKKLDQRNKEVGVRAFVWNIEQMV"
     gene            complement(2949..>5781)
                     /locus_tag="PLJ82_07150"
     CDS             complement(2949..>5781)
                     /locus_tag="PLJ82_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOR41317.1"
                     /translation="VRSSIDLVYDALPDGASRPMIIKLNASMMPVMQYAVTAEDSYPG
                     IKKIIEDNLITVLNRIDGIGNISLLGSPQRYVYVDIDPTKLNSFGLSLETVGSAIANN
                     NMNLASGTVKMGKEQYQLRVQGEYVASEDINNIVVSSTYDGKQIFVRDVAQVRDTIKD
                     VTLEEKINGKDGARLIIMKQSGANTVEIAKKVREQIAQVQKTLPPDIHFEIIYDSSEV
                     IQNAVFGLSETIMYALIFVVFVVLFFLGKWRATFIISLTIPISLIVSFIYLYYTGSSL
                     NIISLSSLSIAIGMVVDDAIVVLENINKHIERGSSPREAAIYATNEVWVSVIATTLVI
                     VAVFLPLTLIGGIAGVMFKELGWIVTIVVCVSTLVAITLTPSLSAMMLTIKKKTENSK
                     TNRFSYQNTVVKWLDKVDELYANILRWCLRNKAITLIVAVLIFIGSLVPVFVGVIGTD
                     FMPQNDEGRLTVTIELQRGTRLEEASKTARIIESRIAKIAPEIQLISTSTGSSDETGF
                     SSLFTSSTNNKIVMMVRTSKKYDRERTIFEIAEKIREDLATLPEVITYDVSTARFGGV
                     MGGNNVEVEIFGHDFDATNAVAAELKQRMEKLSGARNVDIDREEDRPELKIELDKEKI
                     SRHGINTVMMASYVRNRVNGMSAGNFKEQGDEYPILVRLTEEHRNSITSIEELNIATM
                     QGKLLKLKELADIKEYWGPPNIERKRRERIVTVSATPEGVSLGELAEQIKAEVKQISV
                     PRGVQVQVGGDYETQQESFADIATLFLLIVILVYIVMASQFESFSKPFIIMMSVPFAI
                     TGVILALLITGTSLNLIGAIGVVLLVGIVVKNGIVLVDYINLMRDRGNELNEAIALSG
                     QSRLRPVLMTAVTTILGMIPMTLSTSEGSETWVPMGIVVIGGLVVSTLVTLIVVPTLY
                     AVMSRRGERNKEEKLRKSFTFFDKPVSKQ"
BASE COUNT         1867 a         1098 c          863 g         1953 t
ORIGIN      
        1 ggggatttaa tttgcttggt gatattgaaa gcatcaaaaa ttggaatatc atcggtatat
       61 agctttacaa tatcattttt ttcgggagct atcaaaccta cataagcttg tatttcttcg
      121 aaaatagttt tctcattcac ataaatgtgt tgaaaattcg gattaaaact atcacgtaaa
      181 atagtttcag ccctactcat ttctttgagg attaaggatg gaaattgttt ggctttctga
      241 atattttcaa tagcttcttc ccatcttttt tctaaaatct tcagttcagc atctaattct
      301 gccacttttt tgccttcagc aacagtgcga ataataacac cgaaattttt gggttttatg
      361 ctaactaata attgacgaag tcgattgcgt tcttcttccg atttaatttt ttgagaaatg
      421 gaaatcttat ttgaaaaagg caataatact aatgatcgtc cagctataga tatttcggct
      481 gttagtcgag gacccttagt tgatatgggt tctttggtta tttgcaccaa aatttcctga
      541 ccagatttta aaatatcagc aataaagcca tctttctcta agtcaggtaa accagaattt
      601 ttttgcattg aaggtatttt tttcctgtca ttgaccagtg ttttggtaaa ctgtgcaata
      661 gaatggaaat tactacctaa atctaaatag tgcagaaatg catctttatc atatcctaca
      721 tctacgaacg tggcatttaa tccaggcata atttttttaa cttttgctaa aaaaatatct
      781 cctacggaaa aagacatatc acgtggctcc ttgctaaatt ctaccaaagt attattttcc
      841 atcaatgcga tagaaatatc tttagattga acatcaagaa gtaaagtgtt attcatatag
      901 tatataaatg tgatgtattg gtatgcagaa agaacaaact caaaacagat aatttaagtt
      961 tgttcttatt gtgcttagtt gttttagaaa gacaaaaaaa ttattttttc tttttatgtc
     1021 tattttttct tagtcttttt ttacgcttgt gagttgacat tttatgtctt tttctttttt
     1081 ttccgcttgg catatccgta ttcttttata acaattaaat aattttattt tttacagcct
     1141 caacaaaagt tttagcaggc ttaaatgatg ggattgaatg tgctggtata gtaatggtag
     1201 tattacgaga aatattacga gccactttct ctgctctttg ttttacaaca aagctaccaa
     1261 atcctcttaa atacacattt tcgttagaag ctaacgaatc cttcacttca tccataaatg
     1321 attcaactac tctcaacacg gtaaccttat caatacctga tttgtttgcg atttcgttaa
     1381 cgatttctgc tttagtcatt ttagtctatt ttatagttat acttttcttt taaaaacgga
     1441 ctgcaaatat aatactttta gtttcaataa tctacattat tcaaaaaaaa ataatctaaa
     1501 aagttgaatg tttgatttaa tttgaaaaaa tagctacttt tgctacatat aatatacgac
     1561 attgactttt agtgaaataa taatagattg gtacgaggta aataaacgga atttaccttg
     1621 gcgtaatacc aaagatgctt acactatttg ggtatcagaa gttattttac aacaaacgag
     1681 ggtagtgcaa ggtcttgctt attttatacg ttttatcgaa cggtttccaa ctgttattgc
     1741 cttagcacaa gctgatgaag aagaggtttt gaagtattgg caagggttag gatattattc
     1801 gagagctcgt aatatgcaca aagccgcaca gcaggttgtg gagttgtatg atggtatatt
     1861 ccctactcat tacccccact tacttacctt acaaggcatt ggcgaatata ccgcggcagc
     1921 tattagctct tttgcagcca acgaggcaca tgctgtagta gatggaaatg tgtacagggt
     1981 attaagcagg gtattcggta tctttttagc tattaatagc aacgagggca aaagagaatt
     2041 tacgaaatta gcacaatctt tactccccac acatcaagca agcacttata accaggctat
     2101 tatggagttt ggagcgttgc aatgtgttcc tgctcgtcca ttgtgttcaa cttgtccatt
     2161 gcaagttatg tgttatgcat atacccatca acacgtaagt tctttaccca aaaaagagaa
     2221 aaaaactaca acgaaaacac gttatttttt ttacttggat atacgtaatg gtgaatatac
     2281 gtatcttcaa aaaagaacac aaaatgatat ttggcataat ttatttgaat atccactagt
     2341 agaatgtgat aaggaaacat ctatagaaaa cgttatcaat agtgaagatt ttcaagaaat
     2401 agtagaaaaa tgtgagaaaa catctattca cagtatctca agtgaaataa aacatgttct
     2461 aagtcatcaa attatacgta ctatttttat ccgtatacac atagaaactg acaattcact
     2521 actacaatca ttatataaaa taaatagtcg agacatagaa gactatccta tatctcgact
     2581 gatgcataag tatattgaaa agattactaa cgaaacctaa gtaactatac catttgctca
     2641 atattccaaa caaatgctct aacgcctact tctttattac gttgatctag ttttttaaca
     2701 gcaatcaata aatcatctac tttttcgtca ggaatgactg tcataactgc agaattcatt
     2761 tctggccaag tatgtgtacc acgacgcggt tctccattca tactgcctac tccttgcaca
     2821 ttttcccaaa aggtaaagcc tctaatggat aatctatcaa gcatatattc tacacgttct
     2881 gtattagctt gattaaacac aataaatact gacttcatat ggtgtatttt attttctggt
     2941 aattttaact attgtttact cactggttta tcaaagaacg taaatgattt acgtaacttt
     3001 tcttctttat tgcgttctcc tctacgagac atcacagcat acaatgttgg aactacgata
     3061 agagttacta aggtagaaac aaccagacct ccaattacta caatccccat aggcacccat
     3121 gtttcagagc cctcgctagt gcttaatgtc atcggaatca tccccaaaat agtagttaca
     3181 gcggtcatta acactggtcg taaacgtgac tgccctgata aagcaatagc ttcatttagc
     3241 tcatttcctc tatctctcat taaattgata tagtcgacta acacaatacc attttttacc
     3301 acgataccta ccaatagtac cacacctatt gcccctatta aattaagtga tgtcccggta
     3361 atcagcaatg ccaaaataac acccgtaata gcaaaaggaa cggacatcat aattataaat
     3421 ggtttggaga aagattcaaa ctgcgatgcc attacaatgt atactaaaat tacaatcaat
     3481 aaaaacaagg tagcaatatc agcaaacgat tcctgttgtg tttcataatc accaccaact
     3541 tgcacttgta ctcctcgtgg aacactaatt tgttttacct ctgccttaat ttgttcagct
     3601 aattcaccga gcgacacacc ctctggcgta gcagatacgg ttacaatacg ttcacgacgt
     3661 ttacgttcaa tatttggtgg tccccaatat tctttaatat ctgccaactc ttttaatttc
     3721 aataatttac cttgcatcgt agcaatattt aactcttcga tagaagtaat actattacga
     3781 tgctcctctg tcaatctaac caaaatagga tactcatctc cctgctcttt aaaattaccg
     3841 gctgacatac catttactct attgcgaaca taagatgcca tcatcactgt attaatgccg
     3901 tggcgagata ttttttcttt atcaagctct atttttaatt ctggtctatc ttcttctcta
     3961 tcaatatcaa cattacgagc acctgataat ttttccattc gttgtttcaa ttctgccgcc
     4021 actgcattag ttgcatcaaa atcatgacca aaaatctcta cttcaacatt attaccaccc
     4081 ataacaccgc caaatcttgc agtagataca tcgtatgtta ttacttcggg taaggtagct
     4141 aagtcttctc gaattttttc agcaatttca aatatcgttc tttctctatc atatttttta
     4201 gatgtcctaa ccatcataac aattttatta ttggtagaag aagtaaatag agaagaaaaa
     4261 cctgtttcat cagacgaacc tgttgacgtc gatattaatt gtatttctgg agcaatcttg
     4321 gcaatcctgc tttctattat acgtgctgtt ttacttgctt cctccaagcg tgtacctcgt
     4381 tgtaattcaa ttgttacggt aagccttcct tcatcatttt gtggcataaa atcagtacca
     4441 ataactccca caaaaacagg taccaacgaa cctataaata tgagtacagc tacaattaag
     4501 gtaatagcct tatttcgtaa acaccagcgt aaaatatttg catatagttc atctacttta
     4561 tcaagccatt ttactactgt attttgataa ctaaaacgat ttgttttact attttcagtt
     4621 ttctttttga tggttaacat catagcagac aaactaggag ttagcgtaat agctaccaaa
     4681 gttgaaacac atacaacaat ggttacaatc caacctaatt ctttaaacat cacaccagca
     4741 atacctccta tcaaggttaa cggtaaaaat acggctacaa ttaccaaggt agtagcgata
     4801 acagaaaccc acacctcgtt ggtagcgtaa atagccgcct cacgagggct agaacctctt
     4861 tcaatatgtt tgtttatatt ttcgagcacc acaattgcat catctaccac cataccaata
     4921 gctatggata acgacgaaag cgaaataata tttaacgaac ttcctgtgta atacaaataa
     4981 ataaatgaaa cgattaatga tataggaata gtaagtgata taataaatgt tgctctccat
     5041 ttacctaaaa agaaaagtac aacaaatacc acaaaaataa gtgcatacat gattgtttct
     5101 gacaatccaa aaactgcatt ttgtataact tcagaagagt cataaataat ttcaaaatga
     5161 atatcaggag gcaatgtttt ttgtacttga gcaatttgtt cgcgtacttt tttagcaatt
     5221 tctaccgtat tggcaccaga ttgtttcata ataatcaatc gggctccatc ttttccgtta
     5281 atcttttctt ctaaggtaac atctttaata gtatctctaa cttgagctac atctcttaca
     5341 aatatttgct taccatcata cgttgaagaa acaacaatat tattaatatc ttcgcttgct
     5401 acatattctc cttgtacacg caattgatat tgttcttttc ccattttaac ggtacctgaa
     5461 gccaaattca tgttgttatt ggcaatagca gaacctaccg tttctaagct taatccgaat
     5521 gaatttagtt tcgttggatc tatatctaca tatacataac gctgagggga tcctaataaa
     5581 gagatatttc ctatcccatc tattctattt aatacggtta ttaagttatc ttcgattatt
     5641 ttttttatac caggatagct atcttcggca gttacagcat attgcataac aggcatcatg
     5701 ctggcattaa gcttgataat cataggtcgt gaagctccat ctggaagagc atcatacaca
     5761 aggtcgatag aagaccgcac a
//
LOCUS       DAOSRP010000111         5648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13046_length_5648_cov_3.917576, whole genome shotgun sequence.
ACCESSION   DAOSRP010000111 DAOSRP010000000
VERSION     DAOSRP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_5648_cov_3.917576"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1679)
                     /locus_tag="PLJ82_07155"
     CDS             complement(186..1679)
                     /locus_tag="PLJ82_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR41318.1"
                     /translation="MLEIQKKMSNSFFALLSLPATAMGFALSVQISALSWLLSTKYGL
                     DIHEIGIVWAAGPIAGIIAQPIVGALSDKMWFMGGRRRPWIVIGGILAALMMLALPNL
                     EIIQKIFINSEDPQAGLLTIAVIVALTFDLSINVSFNPTRSIIADCTTMKDRSKGYTW
                     MQTISGSFGVLAYLIGGVLGNLFLIYFGVGIALLATLIPAFLIEEPRELAIEIDENEP
                     ITTAKASKRDMIESLLPLSGFLLYGIYVIFEKLFGLHVTAGYTVFGYFLNVVELLCIL
                     LLVVSTIAVLFNLTKSNTKSNEFQRILLAHSFTWIGVQTMFVYAYFYLKEIILLEDKV
                     AGSVNSIAFFILSLVSAILPVLVLNKFAEKIGMVKTHLACIFIMALGYAGVVLFGTSV
                     IPYYVMLGVVGIGWASVVSLPFAIMSSKVDQTKMGLYMGIFNLSVVLPQLVASFKMGE
                     VISGSDDKSIIFVICAITLFISGVLWFFVKEAKVSKTANTPKGGGGH"
     gene            complement(1779..2375)
                     /gene="yihA"
                     /locus_tag="PLJ82_07160"
     CDS             complement(1779..2375)
                     /gene="yihA"
                     /locus_tag="PLJ82_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOR41319.1"
                     /translation="MIIQSATFVISNTDTSKCPNSQLPEYAFIGRSNVGKSSLINMLT
                     GVKNLAMTSSKPGKTLLINHFLINEQWHLVDLPGYGYARIGKQGRERLEEMIRVYITK
                     RVQLTNLFVLIDSRHEPQQIDIEFITWLGEEGIPFSLIFTKSDKLSKSALQLNISQYK
                     EKLYEVWEELPPIFVTSSEKGIGRDEVLQYIAEINASL"
     gene            complement(2372..3844)
                     /locus_tag="PLJ82_07165"
     CDS             complement(2372..3844)
                     /locus_tag="PLJ82_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOR41320.1"
                     /translation="MSAIGLLLLIIIYFGVLQCIAFVGRKEKSNTAFFLGNKTSPWYI
                     VSFGMIGASLSGVTFVSVPGWVLSSGFSYMQMVLGFSVGYIIVAFVLLPLYYRLQLTS
                     IYTYLYERFGKATHTTGSLFFLLSRTIGGATRLYIVALILQRFIFDTWNIPFFITVSL
                     LLALIYLYTYANGIKTIVWTDALQTLFLLIALILLIIQSMDFLSLDINTTVETIKNSS
                     YTRLWIVDDWKNPSHFIKLFISGVFITIAMTGLDQDMMQKNLSCSNTRSAQKNMLSYG
                     MAFIPVNILFLTLGFLLVMITETQGIALPAQTDSLLPNIIADGILGSWAMVFFVIGII
                     AATFSSADSALVSLTTSFAVDIIQIEHMEEQKATTMRRWIHLAFCFVFLVIILLFHLI
                     NNKSILDLLFTIASYTYGPLLGIFTYGIFTKWKISDTLVPYIAVISPVLAYGFFWVFS
                     TYFNYYIGYELLIINGTITFLGMLLFTKKKSLHPTKTIIL"
     gene            3930..4556
                     /gene="recR"
                     /locus_tag="PLJ82_07170"
     CDS             3930..4556
                     /gene="recR"
                     /locus_tag="PLJ82_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR41321.1"
                     /translation="MNNHHYPSILLENAVNQFAKLPGIGRKTALRLVLHLLKQEKSEV
                     GGFAQALVSLKNEIRYCAICKNISDTEVCSICNDTARIRDIVCVVETIKDVMHIENTG
                     QYRGLYHVLGGVISPMDGVGPADIEIESLIQRVQIGEIKEIILALNPTMEGDTTCFYL
                     YKKLLHYPITISTIARGISIGDELQYADEVTLGRSIVHRVPFASSLQL"
     gene            4571..5011
                     /locus_tag="PLJ82_07175"
     CDS             4571..5011
                     /locus_tag="PLJ82_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41322.1"
                     /translation="MEKEIKTIQKTLLKFYYLLYATLMLILIAGGFLLPKFDPLQAVT
                     VQILNTISIALVLVCIPLGLKYFSDKTKKLKNEVTDKQTRIVSYIRYAKQLMFVLGLP
                     GIFAGVSYYLIPEDKTSLFCFLMSFVALVLSKPTEVKLQKFFIE"
     gene            5049..5471
                     /locus_tag="PLJ82_07180"
     CDS             5049..5471
                     /locus_tag="PLJ82_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41323.1"
                     /translation="MIIAVDFDGTIVEHAYPSIGDEIPFAIETLKKIQDEGEHQLVLW
                     TVREGELLQEAVEYCRKKGLEFYAVNKDYPEEEYDIRANRKLKADLFIDDRNVGGLPD
                     WGVIYAMIKGKVKPKYDGGYEIPKRKNVFIHIGEWLQK"
     gene            5505..>5648
                     /locus_tag="PLJ82_07185"
     CDS             5505..>5648
                     /locus_tag="PLJ82_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR41324.1"
                     /translation="MKLSIVIVNYKVPHLLLQCLDAVEKAIQCMDAEVIVVDNNSNDQ
                     SRDL"
BASE COUNT         1989 a          958 c          986 g         1715 t
ORIGIN      
        1 aaggaaagct ccagctttcc ctattcaaat caaactaccg ctacctcacg aaggacgaac
       61 aacgctaaac accttgttaa gtgcatttta atattaagct ttctataact tgcaaatcgt
      121 aactataaaa aaacagcgta accaaatttc gttacgctgt tttcacatat agagtgttac
      181 tttaattaat gaccaccgcc acctttaggg gtattcgctg tttttgatac tttggcttct
      241 tttacaaaaa accataaaac accagagata aataaggtaa tagcacaaat aacaaagatg
      301 atagatttgt catcagaacc agaaataact tcacccattt tgaaactagc tactaactgt
      361 ggcaatacta ctgataggtt aaaaataccc atgtataagc ccatttttgt ttggtcaact
      421 tttgagctca taatagcaaa tggcaacgac actaccgatg cccatcctat acccacaaca
      481 cctaacatta catagtaagg aattaccgat gtgccaaaca acacaacacc agcataaccc
      541 aatgccataa taaatataca tgcaaggtgt gtttttacca taccaatttt ctcggcaaat
      601 ttgtttaata ccaacacagg caatatggca ctaaccaagc ttagtataaa gaaagcaatg
      661 gagttaacac ttcctgctac tttgtcttct aataagataa tttctttcag gtaaaagtaa
      721 gcatatacaa acattgtttg tactccaatc catgtaaagg agtgtgccaa caaaatacgt
      781 tgaaattcgt ttgatttagt attgcttttg gttaagttaa acaacacagc aatggtgctt
      841 acaaccaaca acaaaataca caatagttcc actacattca gaaaatagcc aaatacagta
      901 taaccagctg ttacatgcaa tccaaatagt ttttcaaaaa ttacataaat tccatacaac
      961 aaaaatccag ataaggggag taatgattct atcatatctc ttttggatgc tttggctgta
     1021 gtaataggtt cgttttcatc aatttctatt gccaattctc taggttcttc tatcagaaat
     1081 gctggaatta aagttgccaa taacgcaata ccaaccccaa aatagattaa aaataagttg
     1141 cctagtacac ctccaattaa ataagccaat acgccaaatg atcctgaaat agtttgcatc
     1201 caggtgtatc ctttggagcg atctttcatc gtagtacaat cagcaattat agagcgggta
     1261 gggttaaaac ttacgttgat acttaaatcg aaagttaaag ctacgattac ggctatagtt
     1321 aacaaaccag cttgagggtc ttcgctattg ataaatattt tttgaataat ttctaagttt
     1381 ggtaatgcaa gcatcatcaa tgctgctaaa atacctccaa tcacaatcca aggacgacga
     1441 cgaccaccca taaaccacat tttatcactc agtgcaccta caatgggttg tgcaataata
     1501 cctgcaatag gtcctgctgc ccatacaata ccaatttcgt gaatatctaa gccatacttg
     1561 gtggaaagta accaacttaa tgcagagatt tgtaccgata aggcaaatcc catagctgtg
     1621 gctggaagac tgagaagagc aaagaatgaa ttgctcattt tcttttgaat ttctaacata
     1681 gtatttattt ttaatttata gataatgatt tttttctgca aaatcggaac aaaaatacaa
     1741 ggaaattttg aatctcaaag agttttaact aaaaaaaact acaagctagc atttatctcc
     1801 gcaatatatt gtagcacttc gtcccgtcca atgccttttt ctgatgaagt aacaaaaata
     1861 ggtggaagct cttcccatac ctcgtatagt ttctctttgt attgactaat atttagttgt
     1921 agtgcagatt ttgatagctt gtctgatttc gtaaaaataa gactaaacgg aataccttct
     1981 tcgcctaacc atgtaataaa ttcaatatca atttgttgtg gttcgtgtcg agaatctatt
     2041 aatacaaata aattggtaag ttgcacgcgt tttgtaatgt acacacgtat catctcttct
     2101 aatcgttctc tgccttgttt gccaatacgg gcataaccat atcctggtaa gtcgaccaaa
     2161 tgccactgtt cattgataag aaaatggttg ataagcaatg tctttcctgg tttggagctc
     2221 gtcattgcca agtttttcac cccagtaagc atgttgataa gcgatgattt tcctacatta
     2281 gaacgaccaa taaaggcata ttcgggcagt tgactgttgg ggcatttact ggtgtcagta
     2341 ttacttatta cgaatgtagc tgattgtata atcataatat tattgtttta gtagggtgta
     2401 atgatttctt tttagtgaac aaaagcattc ctaaaaaagt aattgttcca tttattatca
     2461 agagttcgta tccaatataa tagttgaaat aggtggaaaa aacccaaaaa aatccatacg
     2521 ctaacaccgg agaaattaca gcaatatagg gaactaatgt gtcgcttatt ttccatttag
     2581 taaatattcc gtatgtaaat attcctaata acggaccata cgtataggag gcaatagtga
     2641 ataataaatc taagatactt ttattgttaa tgagatgaaa aagcaaaata ataacaagaa
     2701 aaacgaaaca aaaagcaagg tgaatccaac gacgcattgt ggttgctttt tgttcttcca
     2761 tatgttctat ctgtatgatg tctacagcaa aagaggtggt taatgatacc aacgctgaat
     2821 cggcactaga aaacgtggca gcaataatgc ctataacaaa aaacaccata gcccacgagc
     2881 ccaatatgcc atcggctatg atgtttggca gtaaagagtc tgtttgagct ggtagtgcaa
     2941 taccttgtgt ttcagttatc attaccaata aaaaacctaa agttaaaaac aatatattaa
     3001 ccggtataaa agccatacca taactgagca tatttttttg agcacttcta gtatttgagc
     3061 aagatagatt tttttgcatc atatcttggt caagtcctgt catggcaata gtaataaata
     3121 caccactgat aaataatttg ataaaatgag acggattttt ccaatcatca actatccata
     3181 agcgagtgta agagctattt ttaattgttt caactgttgt gtttatatcc aaagataaaa
     3241 aatccatcga ttgaataata agtaaaataa gggcaatgag taaaaataag gtttgtaaag
     3301 catctgtcca aacaatagtt ttaatgccgt ttgcgtaagt ataaagataa atcaaagcaa
     3361 gtaataaact tactgttata aaaaaaggta tattccatgt gtcaaaaata aaacgttgta
     3421 aaataagagc aacgatatac aaacgtgtag ctccaccaat agtgcgtgat aataagaaaa
     3481 ataatgagcc tgttgtgtgt gtcgctttgc caaaacgttc gtataagtat gtatagatac
     3541 ttgtaagttg caagcggtaa tataaaggaa gtaaaacaaa agccacaata atatagccca
     3601 ccgaaaagcc aagtaccatt tgcatataac taaatcctga tgatagtacc caaccgggca
     3661 cagatacaaa cgtaaccccc gaaagggatg ccccaatcat gccaaatgaa acaatatacc
     3721 aaggcgaagt tttgttgcct aaaaaaaatg cagtattgct cttctctttc cttccaacaa
     3781 aagcaataca ttgcaatacc ccaaagtata taataattaa aagaagtaat ccaattgctg
     3841 acatgttttt actaaaatag accacaaagg tattaaaaaa taacaaaaaa gtgatatctt
     3901 tgtagtttga ttagtaagta aatgcttgta tgaataatca tcattaccca tctatattgc
     3961 tcgaaaatgc ggttaatcag tttgctaaat tacctggcat aggtaggaaa acagctctcc
     4021 gattagtatt acatttatta aagcaagaaa aatccgaagt gggtggtttt gcacaggcat
     4081 tagtttcttt gaaaaatgaa atacggtatt gtgccatatg caaaaatata tcagatacag
     4141 aggtatgtag tatctgtaat gatacggctc gtatacgtga tattgtgtgt gtggtagaaa
     4201 caattaaaga cgtgatgcat atcgaaaata caggtcaata tagaggcttg tatcacgtgt
     4261 tgggaggagt tatttccccg atggatggcg ttggtcctgc tgatattgaa atagagagtt
     4321 tgattcaacg ggtacaaata ggcgaaatta aagaaattat tttagcgtta aatccaacca
     4381 tggaagggga tactacgtgt ttttatttat acaagaaatt actccattat cctatcacta
     4441 ttagcactat tgcacgtggt atttcaatag gagacgaatt gcaatatgcc gatgaagtaa
     4501 cattgggcag atctattgtg catcgtgttc catttgcatc ttcattacaa ctataatttt
     4561 aatacaatat atggaaaaag aaataaaaac aatacagaaa acgttgctaa agttttatta
     4621 tctactgtat gccacattga tgcttatatt gatagctggt ggatttttgc tacccaagtt
     4681 tgacccatta caggctgtta cagtacaaat actaaacaca atttctattg ccttggtgtt
     4741 ggtatgtata cccttaggat taaagtattt ttcggataaa acaaaaaagt taaaaaacga
     4801 agtaactgat aagcaaacac gtattgtgag ctatattcgc tatgctaaac agcttatgtt
     4861 tgtgctaggt ttaccaggca tatttgctgg tgtatcgtat tatctgatac cagaagataa
     4921 aacttccttg ttttgttttt taatgtcgtt tgtagcatta gtcctttcta aacctactga
     4981 agttaaattg caaaagtttt ttatagaata aatataagtt tggtacctag tatatataac
     5041 aaatacctat gataatagct gttgattttg atggaactat tgttgagcat gcctatccaa
     5101 gcattggtga tgagatacca tttgccatcg aaaccctaaa aaaaattcaa gacgaaggag
     5161 agcaccagtt ggtgctttgg acagtacgag aaggagaatt attgcaagag gctgtggaat
     5221 attgtcgaaa aaaaggctta gaattttatg ccgttaataa agattatcca gaagaagaat
     5281 acgacataag agccaatcgt aagctaaagg ctgatttatt tattgatgat agaaatgtgg
     5341 gaggactacc tgattggggg gtaatatatg ccatgattaa agggaaagta aaacctaaat
     5401 acgatggtgg ttatgaaatt ccaaaacgaa aaaacgtatt tatccatatc ggagagtggt
     5461 tacagaaata gaagtagtga gttgttgata tttattttta atgcatgaag ttgtctattg
     5521 tcattgttaa ttataaggtg ccccatttgc tgttgcaatg tttagatgct gtagaaaaag
     5581 caattcaatg tatggatgct gaggtaattg tggtggacaa taattccaat gaccaatcac
     5641 gcgattta
//
LOCUS       DAOSRP010000112         5586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13239_length_5586_cov_4.605496, whole genome shotgun sequence.
ACCESSION   DAOSRP010000112 DAOSRP010000000
VERSION     DAOSRP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13239_length_5586_cov_4.605496"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLJ82_07190"
     CDS             <1..410
                     /locus_tag="PLJ82_07190"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOR41325.1"
                     /translation="DYTYNSSGYFAKFGINYNFLKTSTLVPNNIFYIGARYAIASTTY
                     ELATQVAESYWNEQGEVSLMPTKSLATWGEIVLGVRAELFPSVLLGTSVRYGFAPSLS
                     NSLLGYPTFIPNYGLFLPNKWLFTYSITYKLPF"
     gene            493..2535
                     /locus_tag="PLJ82_07195"
     CDS             493..2535
                     /locus_tag="PLJ82_07195"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR41326.1"
                     /translation="MSDIHLVNKAADNIRILSASMVEKAKSGHPGGAMGGADFINILY
                     SEFLVFDPENPRWENRDRFFLDPGHMSPMLYAVLSLMGNYSMDDLKAFRQWGSITPGH
                     PELDVERGVENTSGPLGQGHTMAVGAAIAERFLAARFGDWTSHKIYTFISDGGIQEEI
                     SQGAGRIAGSLGLSNLIMFYDANNVQLSTYVDDITDEDTAKKYEAWGWSVISINGNNA
                     HEIRMALKKANEETQKPTLIIGKTLMGKGSMDANGNSFENKVSTHGQPLTAAGACIRK
                     TIENLGGNADEPFQIFDDVAQLYASRKESLKKIVAQRKLVQAQWEKENPALALKFHSF
                     YANELPNIDFASIVQKPDQATRVASATILGHFSEKIENMIVSSADLSNSDKTDGFLKH
                     TKPIQKGDFTGAFLHAGVSELTMACIMNGLALHGGVIAVCGTFFVFSDYMKPALRMSA
                     LMELPVKYVWTHDAFRVGEDGPTHQPIEQEMQVRLLESLKNHSGKNSMLVLRPADVEE
                     TTVAWKMALENIDSPTGLILSRQNIKNIPRSANPYQEAAQVAKGAYIVKKAMSTPDVV
                     LIASGSEVATLFEAAQLLETRKKLAIQIVSAPSEGLYMNQDASYKESVIPSSTPVFGL
                     TAGLSITLQNLVGYNGRVFGLNHFGYSAPFNVLDEKFGFTPENVYKQVCEYLHIQP"
     gene            2546..2618
                     /locus_tag="PLJ82_07200"
     tRNA            2546..2618
                     /locus_tag="PLJ82_07200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:Lys,seq:ctt)
     gene            complement(2686..3654)
                     /locus_tag="PLJ82_07205"
     CDS             complement(2686..3654)
                     /locus_tag="PLJ82_07205"
                     /inference="COORDINATES: protein motif:HMM:NF039745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6383 domain-containing protein"
                     /protein_id="HOR41327.1"
                     /translation="MKKIFTQKMTILFASVLFSGATAFATVDIEANETYTQSFDGIGV
                     ETTATLPTGWKADKSTSVRTVGTYAAALTATEKAGGNSLSSTAGAGIYNFGAGKLDEA
                     TDRSIGGLTNASAIKNINFYVKLTNNGTTPISSFTISYDIEQYRAGNNGADGTIGAGF
                     SFQMYYSDNDQTYTSAGSDFLTSFAADATTGGYESAPGNTKSVLSKTLNQPLAAGESI
                     YLAWNYSVTSGTYTSYSQALGLDNVSITANAATGIANITNNATVVANNGSIVVTATEG
                     AEIEVYTTAGQKIASAIASGNQDIIAVSAKGIVIVKVGTETIKVVL"
     gene            3860..>5586
                     /locus_tag="PLJ82_07210"
     CDS             3860..>5586
                     /locus_tag="PLJ82_07210"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOR41328.1"
                     /translation="MYNYVKVAAAVPNLEVANCYFNQESICAKIMEARHLGIEVLVFP
                     ELCITGYTCGDLFFQQTLQKATLDALQRILSATESIDMLVAVGAPLCVDNQLFNCAIV
                     LSKGKIVGIVPKTYIPNYNEFYEQRWFSSAFDLKINTINICSQVIPIGTDLLFSLHNG
                     TKIGVEICEDLWVPIPPSAGLCVSGADIILNLSASNETVAKRQYRRNLVVQQSARCMC
                     AYVYASAGGTESTTDLVFSGHSMIAENGVLLKENAQLIDSDYLLSSHVDIDKLRVDRC
                     KYTTYKETHRAYFKEIRCIPSDVQSKHNVSLDYPISRKPFVPSNLHLRDNRCQEIVAM
                     QVAGLVKRLVHTNAPTVVIGVSGGLDSTLALLVCVQAFDELKKDRKGILAVTMPGFGT
                     TNRTYDNALTLIQELGVTRRVIDIKKSCYQHFEDIEHDETIHDITYENTQARERTKLL
                     MNLANKHRGFVIGTGDLSELALGWCTYNGDHMSMYAVNVSIPKTLIRSLVLNIAHTQE
                     EKIKQVLLDILDTPVSPELLPIGKNGEMIQKTEDSVGPYELHDFFLFYVMRYGFSPSK
                     ILFLAKHIFS"
BASE COUNT         1785 a          895 c         1085 g         1821 t
ORIGIN      
        1 ccgattatac ctataattca tcaggatatt ttgctaagtt tgggattaat tataattttt
       61 tgaaaacttc tacacttgtt cccaataata tattttacat aggagcacgc tatgctatag
      121 cctctactac ttacgaattg gcaactcagg ttgctgaaag ttattggaat gaacaaggag
      181 aggtttctct tatgccaacg aagagccttg caacttgggg agaaatagta ttaggagtga
      241 gagcagaatt gtttccatca gtattattag gtacaagtgt tcgttacgga tttgctcctt
      301 cgctctcaaa ttctttgctg ggatatccaa cttttatacc aaattatgga ttatttttac
      361 ccaataagtg gttatttaca tattctataa catataaatt accattttga tatttttttt
      421 tgtagaaagt gattattctt gtaactttgc actttctaat tttttttgaa tcgaaattaa
      481 tatataaatg atatgtctga tatacatcta gtaaataaag cagccgataa catacgtatt
      541 ttatcagcat ctatggtaga aaaagccaaa tctggtcatc caggaggtgc tatgggtggg
      601 gcggatttta ttaatatttt atattctgaa ttcttagttt ttgatccaga gaatccacgt
      661 tgggaaaata gagatagatt ctttttagat cctggacata tgtcgccaat gttgtatgca
      721 gtattgtcat taatgggtaa ctactcaatg gatgatttga aagcatttag acaatggggt
      781 agtattacac caggacatcc cgaattagac gtagaaagag gagtggaaaa tacctcaggt
      841 ccattaggac aaggacatac catggctgta ggtgcagcta ttgcagaacg ttttttagcc
      901 gctcgttttg gcgattggac atctcataaa atttacacct ttatttcaga tgggggaatt
      961 caagaagaga tatcccaagg agcaggtcgt atagcaggtt cgctggggtt gagtaatcta
     1021 atcatgtttt atgatgcaaa taatgtgcaa ctatctacct atgtagatga tataacagac
     1081 gaagatactg ctaaaaaata tgaggcttgg ggttggagtg ttatttctat taatggaaat
     1141 aatgctcatg aaattagaat ggctttgaaa aaagcaaacg aagaaacaca aaaacctact
     1201 cttattatag gaaaaacctt aatgggtaaa ggctccatgg atgctaatgg taatagcttt
     1261 gaaaacaaag tgtcgacaca tggtcaacct ttaactgccg ctggtgcatg tataaggaaa
     1321 acaattgaaa atttgggtgg aaatgctgat gagccatttc agatttttga tgacgttgcc
     1381 caattatatg cttctcgcaa agagagttta aagaaaatag tagctcaacg taagctcgtg
     1441 caagcacaat gggaaaaaga aaaccccgca ttagctctta agtttcattc gttttatgca
     1501 aatgaattac ctaatattga ttttgcttcc atcgttcaaa agccagacca agcaactcga
     1561 gttgcatcag ctaccatatt gggacatttt tctgaaaaga tagagaatat gattgtttct
     1621 tcagctgatt tatccaattc cgataaaaca gatggattcc taaaacacac gaaacctata
     1681 caaaaaggag attttactgg agctttttta catgctggtg taagcgaatt aaccatggca
     1741 tgtattatga atgggttggc tttgcatggt ggtgttattg ctgtgtgtgg cacattcttt
     1801 gtgttttcgg attatatgaa accagccttg cgtatgtctg cattgatgga attgcctgta
     1861 aagtatgttt ggacacacga tgcgtttaga gtgggtgagg atggtcctac gcatcaacct
     1921 attgaacaag aaatgcaagt gcgtttgttg gaatcgctaa aaaatcatag tggaaaaaat
     1981 tcgatgttgg tactaagacc tgctgatgta gaagaaacaa cagtggcttg gaaaatggca
     2041 ttagaaaata tcgattcgcc tacaggactt attttgtcaa gacaaaacat aaaaaacata
     2101 ccaagaagtg ctaatccgta tcaagaagct gctcaggtag caaaaggagc atatatagtt
     2161 aaaaaagcga tgtcaacccc tgatgttgtg ttgattgcaa gtggttcgga agtagctact
     2221 ttgtttgaag cagctcaatt actcgaaaca agaaaaaaac tagcgataca gattgtatct
     2281 gcaccttccg aagggttgta catgaatcaa gatgcatctt ataaagaatc tgttattccg
     2341 tcatcaacgc ctgtgtttgg attaacggct ggtttatcta ttacattaca aaatcttgtt
     2401 ggatataatg gtcgtgtatt tggattaaat cattttggat actctgcacc ttttaatgta
     2461 ttagatgaga agtttgggtt tactcctgaa aatgtttaca aacaagtatg tgagtattta
     2521 catatacagc cttaatgctg atgtagactt cgtagctcag ctggtagagc aattgactct
     2581 taatcaatgg gtcgtgggtt cgagccccac cgaggtcact aaattagggc ataaaaaaag
     2641 agatttgctt ttgcaaatct ctttttttat ttaagcagta ttagattata acactacttt
     2701 tatagtttca gtaccaactt ttacaattac aataccttta gctgaaacag caataatatc
     2761 ttggttgcca ctagctatgg cagatgcaat tttttgacca gcagtagtat atacttcaat
     2821 ttctgctcct tctgttgcag ttacaacaat agaaccattg tttgcaacaa cagttgcatt
     2881 gtttgtaata tttgctatac cagtagctgc attggctgta atagaaacat tatcaagacc
     2941 aagagcttga gaataagatg tatatgttcc gcttgtaact gaatagttcc acgcaagata
     3001 gattgattca cctgcagcta atggttggtt taatgttttg cttagtaccg atttagtatt
     3061 tcctggagct gattcataac ctccagttgt agcatctgct gcaaaactag ttaaaaagtc
     3121 agaacctgca gatgtataag tttgatcgtt atctgaataa tacatttgga aagaaaaacc
     3181 tgctccaatt gtaccatctg ctccattatt tcctgctcta tattgttcta tatcataact
     3241 tattgtaaaa gaagaaatag gagtagttcc attattagta agttttacat agaagtttat
     3301 attttttatc gcactagcat tggttaatcc accaattgat ctatcggtag cttcatctaa
     3361 ttttccggca ccaaaattgt agataccagc accagcagta ctacttaagg aatttcctcc
     3421 agctttttca gttgcagtaa gtgcagcagc gtacgttcct acagttctaa ccgaagttga
     3481 tttatcagct ttccatccag ttggtaaagt agccgtagtt tcaactccaa taccatcaaa
     3541 gctttgcgta tacgtttcat ttgcttcaat atcgactgtt gcaaatgctg ttgctccgct
     3601 aaataagaca gaagcaaaca aaatggtcat tttttgtgta aaaattttct tcataatgtt
     3661 ttaaatttaa tgtgtgaata atttatgtgt ttcctattaa tcacaaatat agggttattt
     3721 ttttttaata aaaaataaat ctccactaaa aacctaacaa ttcatcagaa cttaacaagc
     3781 ttctgttgta attggtactt aaattatcaa caatattaga tattttttat acttttgcaa
     3841 caactaaata tgtcgctata tgtataatta tgtaaaggta gcagccgcag tacccaatct
     3901 cgaagttgca aattgttatt ttaatcaaga atcaatttgt gcaaaaatta tggaagcaag
     3961 acatcttggg attgaggtat tggtttttcc agaattatgc ataacaggat atacttgtgg
     4021 tgatttattt tttcaacaga cactacaaaa agccacttta gacgctttac aacgaatact
     4081 ttctgcaaca gaaagtatag atatgcttgt tgctgtagga gctccattgt gtgttgataa
     4141 tcaactcttt aattgtgcta ttgttttgtc aaaaggtaag atagtaggaa tagtacctaa
     4201 aacgtatatc ccaaattata atgaatttta cgaacagcgt tggttttcat ctgcatttga
     4261 tttgaaaata aacacaataa acatatgtag tcaggtaata ccaataggta ctgatttgtt
     4321 attttcgttg cacaatggca ctaaaattgg tgttgaaatt tgtgaagatt tgtgggttcc
     4381 aattcctcct agtgcaggtc tttgtgtgtc tggagcagat attattctta atttatcggc
     4441 tagtaacgaa actgttgcca aaagacaata tagaagaaat ttagtcgttc agcagtcagc
     4501 acgttgtatg tgtgcctatg tatatgcgag tgctggaggg actgaatcaa caactgattt
     4561 agtgtttagt gggcactcta tgattgcaga aaatggcgtt ttgctaaaag agaacgcaca
     4621 attaatagat tcagattatc tactttcctc acatgtagat atagataagt tacgtgtaga
     4681 tagatgcaag tatacaacct acaaagaaac ccatcgagcc tattttaaag aaattaggtg
     4741 cataccatct gatgttcaaa gtaaacataa tgtatcgctt gactatccta ttagccgaaa
     4801 gccttttgtt cctagtaatt tacacctacg cgataatcgg tgtcaagaaa tagttgctat
     4861 gcaagtagca ggtttagtca aacgtttagt tcatacaaat gctccaactg ttgtaatagg
     4921 tgtatcagga ggattggatt ctactttagc tcttttggtg tgtgtgcaag cttttgatga
     4981 actgaaaaaa gacagaaaag gtatacttgc tgtaactatg ccagggtttg gtactacgaa
     5041 tagaacgtat gataatgctc ttaccttgat tcaggaactt ggagttacac gtagagttat
     5101 tgatattaaa aaatcttgtt atcagcattt tgaagacata gagcatgatg agactatcca
     5161 tgatattact tacgaaaata cacaagcacg ggagcgaact aaactgttaa tgaatttagc
     5221 gaataagcat agaggttttg ttatcggaac tggcgatttg agcgaacttg ctttgggttg
     5281 gtgcacgtac aatggtgatc atatgagtat gtatgctgtg aatgtaagta tacccaaaac
     5341 gttaattcgt agtttagtgt tgaatatagc acatacacaa gaggaaaaga ttaaacaggt
     5401 tttgttagac atattagaca ctccagtaag tccagaatta ttaccaatag gaaaaaatgg
     5461 cgaaatgata caaaaaaccg aagatagcgt aggtccttac gaattgcatg atttcttttt
     5521 gttttatgtg atgcgttatg ggtttagtcc aagtaaaatt ttatttcttg caaagcacat
     5581 attttc
//
LOCUS       DAOSRP010000113         5480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13580_length_5480_cov_4.619908, whole genome shotgun sequence.
ACCESSION   DAOSRP010000113 DAOSRP010000000
VERSION     DAOSRP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13580_length_5480_cov_4.619908"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLJ82_07215"
     CDS             <1..478
                     /locus_tag="PLJ82_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOR41329.1"
                     /translation="KCAIEMPETRLFDGKYFGVQRFDRVQSDKIHVVSAAGLLNADYR
                     TPCLDYTTLFQLCLALTRNAQEMWKLYRLMVFNYVIGNKDDHAKNFSFLYQNNEWKLS
                     PAFDILPSEGINGFHTTTINNTLYPTKQDIIDVAVKAGLNKQKALYIFDEIVATVG"
     gene            1041..1325
                     /locus_tag="PLJ82_07220"
     CDS             1041..1325
                     /locus_tag="PLJ82_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR41330.1"
                     /translation="MTDYLHRIAEKEINETLLAMGAVLIQGARAVGKSTIAKKLAKSD
                     ISLDSSVALIELAKISPNVILTGQTPRFIDEWQLAPSIWNAVRHFVAQVS"
     gene            1574..4162
                     /gene="clpB"
                     /locus_tag="PLJ82_07225"
     CDS             1574..4162
                     /gene="clpB"
                     /locus_tag="PLJ82_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOR41331.1"
                     /translation="MNFEKFTIKSQEAIQKAIELAQTNGQQAIETAHLLKAILTVADA
                     VSQFIVQKLNINIAQLNQSVDGLIRSLPKVSGGQPYVSNETNKVLQTAQSLSEKAGNQ
                     FVSVEYIVLALVKEKSNISQLFKELGVNEQNVQVAINELTKNQKVTSPSAEDTYQALS
                     KYAVNLNEKARSGKLDPVIGRDEEIRRVLQILTRRTKNNPILIGEPGTGKTAIAEGLA
                     HRIVRGDVPENLKSKQLYSLDMGALIAGAKYKGEFEERLKSVVNEVVNADGEIILFID
                     EIHTLVGAGKSDGAMDAANILKPALARGELRSIGATTLDEYQKYFEKDKALERRFQIV
                     MVNEPDEASTISILRGLKERYENHHKVRIKDDAIIAAVELSNRYISDRFLPDKAIDLI
                     DEAAAKLRLEVDSVPEELDKIERTIKQLEIEREAIKRENNEQKLTQLSTEIANLKEEE
                     KEQKAKWKAEKELIESIQQNKIDIEQLKFEADKAEREGDYGKVAEIRYGKIKQKEEEI
                     ANFQQKLVSLQADHPIIKEEVDSEDIADIVSRWTGIPVSKMVQSEKDKLLHLEAELHK
                     RVIGQHEAITAIADAVRRSRAGLKDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRIVNFKNTIIIMTSNIGSSLIRENFEHITASNRDNVIE
                     NTKNEIFELLKKSIKPEFLNRIDDLIMFTPLSEEEIHQIVGLQIKLIANMLHKNDIDF
                     SVTDKAIDLIAKIGYDPQFGARPIKRVLQRYVLNELSKQMIAENIDKSKPIIVDVEAG
                     ELIFKN"
     gene            complement(4253..5332)
                     /locus_tag="PLJ82_07230"
     CDS             complement(4253..5332)
                     /locus_tag="PLJ82_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOR41332.1"
                     /translation="MKDKLVTLAIHTIEQALQLQHRLEKEGIPSFIKKIRSISTGVRV
                     RVREVDLQKAIYLVERIKSTSNEHIEFKSPFILVPIDFSEHAIHAAKLAFYLAENLHS
                     SIVFLHVETYVNVATFPTLLSSADEKSRLVTKKIKERNEAIIDVENRFKSLIVNQLIP
                     NIHFNFELKNGIPEEEIISFAMLYKPLLIVMGTRSKANKEQELIGSVTAEVIERSLVP
                     VFVLPQHTHLQTIQQIKHIGFATNFDKKELVAFEKLMYFVQKLQFKVSFIHVDDKEDS
                     QNKNKLDELKSYFLTVYPHLQLQFSMLISEKSTLKELENCIRDQKIDILAIRNHKRGM
                     FVRLFAPSLARKLVYQAKNPMIVFY"
BASE COUNT         1970 a          860 c          980 g         1670 t
ORIGIN      
        1 aaaatgtgct atagaaatgc cagaaacgcg tcttttcgat ggaaaatatt ttggagttca
       61 acgatttgat agagtacaat ccgataaaat acatgtggta agtgctgccg gattgctgaa
      121 tgctgactat cgtacaccat gtttagatta tacaaccctt ttccaactat gtctggcttt
      181 gacccgtaat gctcaagaaa tgtggaaatt gtaccgatta atggtattca attatgttat
      241 agggaataaa gatgatcatg caaaaaactt ttcattttta taccaaaata atgaatggaa
      301 attgtctcct gcatttgata tcttaccaag tgaaggaata aatggatttc ataccacaac
      361 aattaacaat acattatatc caacaaaaca agatatcatt gatgtggcag taaaagcagg
      421 tttaaataag cagaaagccc tatatatatt tgatgaaata gtagccactg tgggatagct
      481 taaaaaaaag ctcttccttt cctttatctt cccctcgctt tcagcgaggg gatttttgtt
      541 ttagcgtttt gaacgcattc cactaatgat atattgtttt caacgagcga gatagtgagc
      601 atttcaaaat agtaaatact tcgcaaagtg cataacacaa aaagcgatgt tgaaaataat
      661 tcaccattaa ctcctcgttg aaagcagggt tgaggagtat aagccatagc aactaggata
      721 tcatttgtta taatatgaaa agcaattaag taaggtacat aatgaatctt aagtaataga
      781 ttcttatgta caaatataaa gcaattcaat agtatgtaat tacaaattta gtaaagcaca
      841 aatgcagtaa ttgtgagagt tgtgatgaga cataatcata atagataaat aatgttgtga
      901 attattttca ggcaataatg atataatctg atgcaaaatc aatatcattg taaacgtaaa
      961 agtgatattg atatgaacgc aaaagtgata ttaatatgaa cgcaaaagtg atattaagtt
     1021 gaatataata ctgatatttc atgactgatt atttacacag gatagccgaa aaggagataa
     1081 atgaaacctt attggcaatg ggtgctgtac ttatacaagg agcacgtgct gtaggtaaat
     1141 ctaccattgc aaaaaagtta gcaaaaagtg acatttcact cgactcttca gtagccttga
     1201 ttgagcttgc aaagattagt cccaatgtga ttttaactgg gcaaacaccc cgttttatcg
     1261 acgagtggca attagctcct tcgatttgga acgcagtacg acactttgtg gcgcaagttt
     1321 cataacttgt gccttcgttt caatctctct tctcctcgtt agctataaag atattattgt
     1381 gtgcaactgg tgtaagtgta tttaggtata tatctttgtc taaaatattg aaatacaact
     1441 atttataatg aaaaattagc tataaataaa cccttatcaa cgtttaataa tgtatatttg
     1501 ttctgtcaaa ttgtcagtta tatattctcg gcaaactttt tgaaggttga tagtaaaaaa
     1561 ggaggatata cctatgaatt tcgaaaaatt tactatcaaa tcacaagaag ctattcaaaa
     1621 agccattgaa ttagctcaaa caaatggtca acaagccatt gaaacagctc atttactcaa
     1681 agctatactg actgttgcag atgctgtaag ccaatttatt gttcaaaaac tgaatatcaa
     1741 catcgcacaa ctcaatcagt ctgttgatgg tcttatacgc tcgttaccca aagtttctgg
     1801 cgggcaacca tatgtaagca acgaaaccaa caaagtactt caaacagcac aatcattatc
     1861 cgaaaaagca ggaaatcaat ttgtttcggt agagtacatt gtcttggcac tggtgaaaga
     1921 aaaatccaat atttctcagc tttttaaaga attgggtgta aacgaacaaa atgtgcaagt
     1981 agctataaat gagttgacta aaaaccaaaa agtaacttca ccttctgccg aagataccta
     2041 tcaggctctt tctaagtatg cagtaaactt aaatgaaaag gcacgttcag gtaaacttga
     2101 cccagtaatt ggtagggatg aagaaattcg ccgagtattg caaattctta cccgtagaac
     2161 aaaaaacaac ccaattttaa ttggagaacc tggaacgggt aaaactgcca tagccgaagg
     2221 tttagctcac cgcatagtaa gaggagacgt acctgaaaac ttaaagtcaa aacaactcta
     2281 ttctttggat atgggagctc ttattgctgg agctaaatac aaaggagaat ttgaagaacg
     2341 tttaaaatcg gtggtgaatg aggtagtaaa tgccgatgga gagattattc tatttattga
     2401 tgaaattcac accttggttg gtgcaggtaa aagtgatgga gcaatggatg ctgccaatat
     2461 tctaaaaccc gctttggcaa gaggagaatt acgctctata ggtgctacaa cgctagatga
     2521 ataccaaaaa tattttgaaa aagataaggc attagaacgc cgttttcaga tagtaatggt
     2581 aaacgaacca gatgaagcta gtaccatttc tattttacgt ggattaaaag agcgttatga
     2641 aaatcatcat aaagtacgta tcaaagatga tgccatcatt gctgctgttg agctatctaa
     2701 taggtatatt agcgatcgct ttttgcccga caaagccatt gatttaattg atgaagctgc
     2761 tgcaaaattg cgtttggagg ttgattctgt acccgaagaa ttagataaaa ttgagcgaac
     2821 aatcaagcaa ttagaaattg aacgtgaggc tattaaacgt gaaaacaatg aacaaaaact
     2881 cacacaatta tccaccgaaa tagcgaacct gaaagaagaa gaaaaagaac aaaaagctaa
     2941 atggaaagcc gaaaaagagc ttatcgaatc tattcaacag aataagattg atattgaaca
     3001 attgaaattt gaagcggata aagctgaacg tgaaggcgac tatggcaaag tagcagaaat
     3061 taggtacggg aaaattaaac aaaaagaaga agaaatagcc aattttcaac aaaagttggt
     3121 atcactacaa gctgatcatc caataataaa agaagaagtt gatagtgagg atattgctga
     3181 tattgtgagc cgctggacag gtattcctgt aagcaaaatg gtacaaagtg aaaaggataa
     3241 actattgcat ttggaagcag agttgcacaa acgagttatt ggacaacacg aagccattac
     3301 agccatagca gatgctgtgc gaaggagtag ggcaggtctc aaagacccaa aacgccccat
     3361 aggctcattt attttcttag gtactactgg cgttggtaaa acagagttag ccaaggcttt
     3421 agctgagttt ttgtttgatg atgaaaatat gatgactcgc attgatatga gtgaatacca
     3481 agaaaaattc tcggtaacac gattaattgg ttcacctcca ggatatgtag gctacgacga
     3541 aggtggtcag ctaacagaag ccattcgccg caagccatat tctgtcgtat tattcgacga
     3601 aattgagaaa gcacatcctg atgtatttaa catattgttg caagtattag atgatggtcg
     3661 cttaactgat aacaaaggac gtatcgttaa ttttaaaaac acaattatca ttatgacatc
     3721 aaatataggt tcgtccttaa tacgtgagaa ttttgaacat attactgcat ctaatcgtga
     3781 taatgtgata gaaaatacga agaatgagat ttttgagcta ttaaaaaaga gtatcaaacc
     3841 cgaattcttg aatcgtatag atgatttaat tatgtttacg ccattgtcag aagaggaaat
     3901 acatcagatt gttggcttac aaataaaatt aattgccaat atgcttcaca aaaatgatat
     3961 agatttttct gtaacagata aagcgataga tttaatagca aaaattggtt acgatccaca
     4021 atttggagca cgaccaataa aaagagtatt acaacgatat gtactcaacg aattgtcgaa
     4081 acaaatgatt gccgaaaata ttgataaatc taaaccaatt attgtggatg tagaagcagg
     4141 ggagctaatt tttaaaaact aatagtacta ataaataaag cgacagaaat acaactgtcg
     4201 ctttatttgg atatatccta tttaacataa tattaattat gaaacaattt aattaataaa
     4261 atacaatcat cggatttttc gcctgataaa ctagtttgcg agccaaacta ggtgcaaaca
     4321 aacgtacaaa catccccctc ttatgattgc gtattgccaa tatatctatt ttttggtcac
     4381 ggatacaatt ctctagttct ttaagggtcg atttttcact tataagcatt gaaaactgaa
     4441 gttgtaaatg aggataaaca gtaagaaaat aggattttaa ttcatccaat ttatttttat
     4501 tttggctatc ttctttatca tctacatgaa taaagctaac tttaaattgt aatttttgca
     4561 caaaatacat caatttttca aaagcaacca actctttttt atcaaaatta gtggcaaatc
     4621 ctatatgttt gatctgttgt atcgtttgta aatgtgtatg ctgtggcaat acaaaaactg
     4681 gtaccaaact acgttctatt acctctgctg ttacactccc gataagctct tgttctttat
     4741 tagctttgct tcgtgttccc ataacaatga gtaacggctt ataaagcatt gcaaaagaga
     4801 tgatttcttc ttcaggaatt ccattcttaa gttcaaaatt aaaatgaata tttggtatta
     4861 attgatttac aatcaaagat ttaaaacgat tttccacatc tattatagct tcgtttcgtt
     4921 ctttaatttt ttttgttacc aaccgtgatt tttcatctgc tgaggataat aaagttggaa
     4981 aagttgctac atttacgtat gtttctacat gcaaaaaaac gatagatgaa tgtaagtttt
     5041 ccgctaaata aaatgctagt ttagcggcat gaatagcatg ttcactaaaa tcgatgggaa
     5101 ctaaaataaa tggagattta aattcaatat gttcatttga ggttgatttg attctttcta
     5161 ctaaataaat tgctttttgc aaatcaactt cacgtactcg tacacgtact cccgtagaaa
     5221 tactccgtat tttctttata aaagatggaa taccttcttt ttcgagtcta tgttgcaatt
     5281 gtaatgcttg ttctattgta tgaattgcta gtgtaactaa tttatctttc atatatagta
     5341 atagttagtc agtaaaagta atggatattt tttaaaacag caaaaaataa aatatttttt
     5401 ctaaatttgt agcattaaaa aagaacataa tccttagagt aaatttgtat gcaacacgct
     5461 cccattgatc ctgcaatagt
//
LOCUS       DAOSRP010000114         5425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13749_length_5425_cov_4.529981, whole genome shotgun sequence.
ACCESSION   DAOSRP010000114 DAOSRP010000000
VERSION     DAOSRP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13749_length_5425_cov_4.529981"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..882
                     /locus_tag="PLJ82_07235"
     CDS             121..882
                     /locus_tag="PLJ82_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOR41333.1"
                     /translation="MIRVGLFGFGKTGKMVAKELLQDDLIELVWVVRRNVISEEKYAS
                     RLIGINENKGMIISKERLTHSFYEEYPVDVIIDFSDASAYKNYAIPASKGIKIISAIS
                     HYSPKALEYIKDLSHQTAIIYSPNITLGVNFLMVISQILQKIAPNADIEIVEEHFREK
                     KGISGTACKMAQILGLDEERHVNSIRVGGIVGKHQVIFGLQNQTIRLIHESISGTAFG
                     KGAIFAAKWIVNKQKGYFSMEQIFSDMIQQNIPVY"
     gene            complement(886..1551)
                     /locus_tag="PLJ82_07240"
     CDS             complement(886..1551)
                     /locus_tag="PLJ82_07240"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOR41334.1"
                     /translation="MDVKIEPSWKEILSAEFEEKYFVELVNFVKHEYGINQVYPKAKN
                     LFNAFNLCPFDSTKVVIIGQDPYHGEGQAHGLCFSVPDHTPIPPSLQNIFKEIKNDLE
                     ITPFNTGNLERWAKQGVLLLNATLTVRANVAGSHQGKGWEIFTDKVIQLLSKKKKNLV
                     FILWGSYAQQKKELIDTHNHCIFTSTHPSPLSAYRGFFGNKHFSQTNTYLIDHGKEPI
                     DWR"
     gene            complement(1558..2436)
                     /locus_tag="PLJ82_07245"
     CDS             complement(1558..2436)
                     /locus_tag="PLJ82_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOR41335.1"
                     /translation="MNKEKDSKIKGLIGTVLFHAILLFILLFVVLSREIPTEEEGLTV
                     NFGVTKDGSGYFEPTAQQEIQEQLEELSPPPPTPSQPSVSQEALQTQNFEESLAMKEA
                     KKKQEEEKRKLDEQKRIIAEQKRIEEEKKKEEQRRLEEERKKQAEKIQRATSAGKSAF
                     GNAGANTANTSQSEGTGTGTGNQGNPFGEVDGAHRDGAGRGNNPSFSLSGRSILGQIP
                     LPNYNNNEQGIIVVDIEVDKNGNVISARAGGKGTTIGNITMRKEAEVAAKRAKFNGIT
                     DNINQTGTITYRYVLR"
     gene            complement(2475..2888)
                     /locus_tag="PLJ82_07250"
     CDS             complement(2475..2888)
                     /locus_tag="PLJ82_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOR41336.1"
                     /translation="MALKKRNKVNASFSMSSMTDLVFLLLIFFMITSTVVTPSAIKIL
                     LPQSNNQTVAKPITRVIIDENLNYYIATGREKERAVDFSEIEPYLQSIVLDEPDMYIA
                     LHADESVPHREIVRVLNIANENKFKLVIATRPVKK"
     gene            complement(2929..3630)
                     /locus_tag="PLJ82_07255"
     CDS             complement(2929..3630)
                     /locus_tag="PLJ82_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOR41337.1"
                     /translation="MNLTSILLQVVNNVANTPEVLAETPQVAEMNLFEMALKGGWIMV
                     PIVLLSILAGYIFIERLLIIKKASEEDKTFMNRIKDYILEGKIESATKLCQQNDTPSA
                     RMIEKGISRLGKPMNDVLVAIENVGNIETNKLEKGFAMLATISGGAPMLGFLGTVMGM
                     VRAFFDMANAGSNVDITILSNGIYTAMITTVAGLIVGILAYFAYNYLVSRLDSVVQKM
                     ELRTMEFMDLLNEPI"
     gene            complement(3664..4377)
                     /locus_tag="PLJ82_07260"
     CDS             complement(3664..4377)
                     /locus_tag="PLJ82_07260"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOR41338.1"
                     /translation="MTKLSVNINKVATIRNSRGGNLPNVLQVALDCERYGAEGITVHP
                     RPDERHIRYSDVLELKPALKTEFNIEGYPQQSFMDLVLRIKPAQVTLVPDPPQALTSS
                     EGWDTIEYQQFLTDIVSTLQKEGIRTSIFVGTNPAHSEYAAKTGTDRVEFYTGPYAHD
                     FIIDKRKAIAPFIKAAEVANKNGLDINAGHDLNLENLHYFFTHIPNLKEVSIGHALIS
                     DALYLGLEKTIRYYKNLLQ"
     gene            4524..5411
                     /locus_tag="PLJ82_07265"
     CDS             4524..5411
                     /locus_tag="PLJ82_07265"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOR41339.1"
                     /translation="MKVIVLGNVDKTDFLYTTNELLCALEKYKVEILVGEIEYNYLFA
                     NKQYLPNSVSVYSPNQDVDADLAFSLGGDGTFLTSASIIGNKLIPIMGINMGRLGFLA
                     DVPNNSIAEMVDEIFSKGYKTENRTLLKLSTSSKDIFQDDTALNEIAVLKLDSSSMIM
                     IHATINKEYFCSYQADGLILSTPTGSTAYALSVGASILDPTNQSFILAPVAPHSLNLR
                     PFIFPDTWEVDLEIESRNNSFLVSIDGRSYMMDQATKLNIKKAPYSIIIAKRLEDTYF
                     QTLRNKLMWGADTRHKKPF"
BASE COUNT         1626 a          892 c          950 g         1957 t
ORIGIN      
        1 caaggggttt cttttacttt tgaagaatct tcggaaatct ctttcattaa tttagtggat
       61 tcttttaaca tgatcttcta caacatcgag ttgtgcataa ctagttgtgt ataaacaacc
      121 atgattagag taggactttt tggatttgga aaaacaggaa aaatggtggc taaagaatta
      181 ttacaagacg atttaattga gctagtatgg gttgtgcgaa gaaatgttat atcggaagaa
      241 aaatacgcca gtcgcctaat tggtataaat gaaaataagg gaatgattat ttcgaaagaa
      301 agactaacac attctttcta tgaggagtat cctgtagatg ttataataga tttttctgat
      361 gcaagtgcgt ataaaaatta tgcgatacca gcttcaaagg gtataaaaat aatatcagct
      421 atatctcatt actcccccaa agcccttgag tatattaaag atttgtcaca tcaaacagct
      481 attatatatt ctcccaatat tactttaggt gtaaattttt tgatggtaat atctcagata
      541 ttgcaaaaaa tagcacctaa tgcagatatc gaaattgtag aagaacattt tagggagaag
      601 aaagggattt caggaactgc ttgcaaaatg gcacaaatct tgggtttaga tgaagaacgt
      661 catgtaaatt ctattcgagt aggaggtatt gttgggaaac atcaagttat atttggctta
      721 caaaatcaaa ctattcgtct tattcacgaa agtattagtg gtacggcttt tggtaaaggt
      781 gcaatttttg ctgcaaaatg gattgtgaat aaacaaaaag gttatttttc gatggagcaa
      841 attttttctg acatgataca acaaaatatt cctgtttatt aattattatc tccaatctat
      901 aggttctttt ccatgatcta taagatatgt attggtttga gaaaagtgtt tattgccaaa
      961 aaatcctctg taggctgaca agggagaagg atgtgtggat gtgaaaatac aatggttgtg
     1021 agtatcaatt agttcttttt tttgttgtgc gtaactgccc cataatataa agactagatt
     1081 ttttttcttc tttgaaagta gttgaattac tttatcagta aagatttccc agccttttcc
     1141 ttgatgagaa ccagctacat tggcacgtac tgttagagtg gcatttaata ataatactcc
     1201 ttgttttgcc catctttcga ggttgccagt gttaaagggt gtaatctcta agtcattttt
     1261 aatttctttg aagatatttt gtagtgaggg tggtatggga gtgtggtctg gaactgaaaa
     1321 acataaacca tgagcttgtc cttctccatg ataaggatct tgacctatta ttaccacttt
     1381 tgttgaatca aatggacata aattaaaggc attaaataaa tttttagctt tgggataaac
     1441 ttgattgatg ccatattcgt gtttcacaaa gtttactaat tctacaaaat atttttcttc
     1501 gaattcagca gacaaaattt ctttccaact aggttcaatt ttgacatcca tgacttatta
     1561 tcttaatacg tatcgataag ttatcgttcc tgtttggttt atattatcgg taataccgtt
     1621 aaattttgcc ctttttgctg ccacctcagc ttcttttcgc atagtaatat tcccaattgt
     1681 tgttccttta cccccagctc ttgcactaat aacattacca ttcttatcta cttctatgtc
     1741 aactacaatt ataccttgtt cgttattgtt gtaattaggt aaaggtattt gtcctaatat
     1801 gcttctacca gataaactaa acgaagggtt attacctcga ccagcaccat cacgatgtgc
     1861 tccatctact tctccaaaag ggtttccttg attgcctgtt cccgtacctg ttccttctga
     1921 ttgggatgtg tttgctgtat tagcacctgc attgccaaaa gcactttttc ctgcagatgt
     1981 agctcgttgt attttttctg cttgtttttt tcgttcttct tctaaacgtc tttgctcttc
     2041 ttttttcttt tcctcttcaa ttcgcttttg ttctgcgatg attcgttttt gttcgtctaa
     2101 ttttcgtttt tcttcttctt gtttcttttt tgcttccttc atcgctaatg attcttcgaa
     2161 attttgtgtt tgcaaggctt cttggctaac actgggttga gaaggagtag gaggtggagg
     2221 cgaaagttct tctagttgtt cttggatttc ttgttgtgct gtaggttcaa aataccctga
     2281 cccatcttta gtaacgccaa aattaacggt taatccttcc tcttcggtag gtatttcacg
     2341 tgagagtact acaaataata agatgaacaa cagaatagca tgaaacaaaa ctgttccaat
     2401 tagacctttt attttgctat ctttttcttt attcactgat ggtttatctt aatattaaca
     2461 attagttttt cctattattt ttttacaggt ctcgtagcaa tcaccaactt aaatttattt
     2521 tcgtttgcga tattgagtac ccttacaatt tctcggtgag gcacagattc atcagcatgt
     2581 aatgcaatat acatatcagg ttcatcaaga acaatagatt gcagatatgg ctcaatctct
     2641 gaaaaatcaa cagcacgttc tttttctcta cccgttgcta tgtagtaatt taagttttca
     2701 tcaataatta ctcgtgtaat tggttttgca actgtttgat tattactttg tggtagcaat
     2761 atcttaattg cactaggtgt aacaaccgtt gaagtaatca tgaaaaatat gagtaataaa
     2821 aacaccaaat ctgtcattga agacatgcta aatgatgcat ttactttatt ccgttttttt
     2881 aatgccataa cctatatgat ttatgcaaat ggatgtatat attttttttt aaataggctc
     2941 atttaacaaa tccataaatt ccatggtacg taactccatc ttttgaacaa cagagtctaa
     3001 tctagaaact aagtagttgt atgcaaaata agctaagata ccaacaatta atcctgcaac
     3061 agtcgtaatc atagcggtat agataccatt agatagaata gtaatatcaa cattgctacc
     3121 agcatttgcc atatcgaaaa aggcacgaac catacccatt acagttccca aaaaacctaa
     3181 cataggagcc cctccagaga tggtagccag catagcaaat cctttttcta gtttgtttgt
     3241 ttcaatgttt ccaacgtttt caatggctac taacacgtca ttcattggct tgcctaaacg
     3301 cgagatcccc ttttcaatca ttcgagcaga aggagtgtca ttttgttggc aaagttttgt
     3361 tgccgattca attttgcctt ccaagatata gtctttaatt ctattcataa aggttttatc
     3421 ttcctctgat gcttttttta ttataagtaa tctttcaata aaaatgtaac ctgctaatat
     3481 agaaaggagt acaataggta ccattatcca tccacctttc aaagccattt caaataaatt
     3541 catttcggct acttgaggag tttcagcgag gacttctgga gtattggcaa cattgttaac
     3601 aacttgaagt aaaatacttg taaggttcat ggtgttgagg attttaaatt gttagtagtc
     3661 tatttattgt aagaggtttt tatagtagcg aattgttttt tctaaaccta aatataaggc
     3721 gtcagaaatc aatgcgtgcc caatggatac ttcctttagg tttggtatat gtgtgaagaa
     3781 atagtgtaaa ttttctaaat ttaaatcatg accagcatta atatccaagc catttttgtt
     3841 tgctacttct gccgctttta taaatggtgc tattgctttg cgtttgtcga ttataaaatc
     3901 atgtgcatac ggacctgtat agaactctac tctgtctgtt cccgttttag ctgcatattc
     3961 tgagtgagct gggttagtcc ctacgaaaat agatgttcta atgccttctt tttgtagtgt
     4021 tgatactata tccgttaaaa attgctggta ttctattgtg tcccatcctt cgctagaagt
     4081 aagggcttgg gggggatctg gcactaatgt aacttgtgct ggttttatac gcagtactaa
     4141 gtccataaat gattgttgtg gatatccttc aatattaaac tcagttttaa gagcaggttt
     4201 aagctctaac acatcactat atcgtatatg acgttcatca gggcgtggat gaacggtaat
     4261 accctcggct ccatatcgtt cgcagtccaa tgctacttgt aacacatttg gcaaattacc
     4321 accacgggag ttacgtatgg tagctacttt gtttatattt acgctaagtt ttgtcattta
     4381 cttgtaaaaa atcatttttt ttgctgatat ctcaaaacaa atgtgttatt ttgcatacca
     4441 gtctggtatt aaacgcacaa aattacgatt ttattgcgta aattcatact gtgaatgatt
     4501 aaaaaaataa aatttcctgc aaaatgaaag taattgtatt agggaacgtt gataaaacag
     4561 actttctata cacaactaat gagttattgt gtgctttaga aaaatataaa gtggaaatac
     4621 ttgtcggtga aatcgagtat aattatttgt tcgccaacaa acaatattta ccaaattcag
     4681 tttcagtata ttcgcctaat caagatgttg atgctgattt ggcatttagt ttgggtgggg
     4741 atggtacatt cttaacttca gcttctatta ttggaaataa attgatacct ataatgggta
     4801 ttaatatggg tcgtttgggc tttttggctg atgtccctaa taattcgatt gctgaaatgg
     4861 tggatgaaat ttttagtaag ggatataaaa ctgagaatag aacgttgtta aaattgtcaa
     4921 cttcatctaa agatattttt caggatgata ccgcattaaa tgaaattgct gtattaaaat
     4981 tagactcatc atcaatgatt atgatacatg caacgattaa taaagaatat ttttgttcgt
     5041 atcaagcaga tgggttaatt ctttctacgc caactggatc gacagcctat gcattgagtg
     5101 taggagcttc tattttagac cctactaatc agagttttat actagcacca gtggctcctc
     5161 atagtttaaa tttacgtccc tttattttcc ctgatacatg ggaggtagat ttggaaatag
     5221 aaagtagaaa taatagtttt ttagtatcta ttgatggtcg ttcgtatatg atggatcaag
     5281 ctactaagct gaatattaaa aaagctcctt attctattat aatagcaaaa agattggaag
     5341 atacttattt tcaaactttg cgaaataaat taatgtgggg agcagatact cgtcataaaa
     5401 aaccttttta gcatgttagc ctata
//
LOCUS       DAOSRP010000115         5368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13941_length_5368_cov_4.748918, whole genome shotgun sequence.
ACCESSION   DAOSRP010000115 DAOSRP010000000
VERSION     DAOSRP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_5368_cov_4.748918"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="nspC"
                     /locus_tag="PLJ82_07270"
     CDS             complement(<1..1055)
                     /gene="nspC"
                     /locus_tag="PLJ82_07270"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOR41340.1"
                     /translation="MNLSQIPSPCFVLDEKLLRANLLLIQSVKEQAEVEIILAFKAFA
                     MWKVFPIIREYIPYSTASSLSEARLAYEEMGSKAHTYAPVYKPEEFDAIAACSSHITF
                     NSVAQYNQYAAVCKSKSISCGLRINPEYSSVETDLYNPCAVGSRLGVSVANLDDNLPQ
                     GIEGLHFHTLCESSSFDLEATLEVVEQKFGHFFSQIKWLNMGGGHLMTRKGYDTEHLI
                     NLLKSFKSKYPHIQLILEPGSAFLWQTGVLVSTVLDIVENRGVSTAMLDVSFACHMPD
                     CLEMPYKPRIVGATDVIEGKPTYRMGGNSCLSGDFVGEWSFEQPLKVGDKIVFEDMIH
                     YTMVKTTMFNGVSHPSIG"
     gene            1304..>5368
                     /locus_tag="PLJ82_07275"
     CDS             1304..>5368
                     /locus_tag="PLJ82_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HOR41341.1"
                     /translation="MRKFTTTHQLRFGLIVLSLFSFLASHAITIYFKDTNNSWGGVNI
                     HVWNATISSDKTSGWPGKAMIWKATNGSDTWYKIELQSTTASFKFNRIADNNTQTSNF
                     ENITIDGWYDSGSSGPQNISGVPSGLNFELSLGAVTMSSDALDGWGVRTATTNAGGGI
                     FTLVKLYTQNLGIDIWYGTSKHAHIATTQIIGHSSSMVGKLATFTWNANTNVLSIAAG
                     ITITSNLPSPIDAGTNVTLTASATNEPSNYVWEYSINNGGTWLPYTGEKTGEKDRSIT
                     VTPSTTTIYRAIMGSDNETLELLVKKACAHETRTLFIDDFGTLVDQKDRTTSPYVPAT
                     NYTYKPSGKEIRDSHYAVVTTPRFAGCGTQTGDNVAGCNVAGDYWYVDLKDRSNRTTT
                     PFGGMLFVNCRNKDADVAKNVVYTRQTINLPPNVELTFSAWFANAQNPTRGTKVDINM
                     RFQILNASNTVVSGSIIDVPAIAAGTGWFYGETTINTGSGGLFTVQILNNGASGVGND
                     VLIDDIELKVCVPQVSLNVDGQTNVTVTGLHATSPLTVPESVSSLFSSPYYLWVEYNP
                     TTQTWEEVTAFSGVNKPTNNTSFTSYTPSTKQFKVYVASDRQSALDGFNLGTGLYIVT
                     NPVSLDLCDIITQPSASGEEVCEGTPATALSVVATSGSGTLSYQWYSNTTASNVVGGT
                     LITGAESSTFTPPTTSAGSTYYYVVVSSTNGCSMTSNVSGEIKVNPKIMPTFNAVGPY
                     CSGDIIPALSTSSTNSPAITGTWSPAISNTATQTYTFTPTAGACANTTILEIVVNEKP
                     NTPSIDVDCTLGFGKGVLTVTAPLDVSFEYALNGGTYQDSPTFTGIANGNNSIVVRNK
                     TTQCTSTATNLNVDCGCASLPTVSFGTANGSTCHTNAITITGNTFGGSTTEVTITHNG
                     SGILTDATFTSSPFNVTYTPDPSDAGKTIEITAITNNPLGLPCIASSAIYELTIESLI
                     TPTFTAIDAICQLDNLNPLPTTSLNGINGSWSPALDNTQTTEYTFTPDAGQCASEVKM
                     TIIVHDKPAQPTATVTQPTCATLRGAIDVNNHNHVDGLTYSINGVDFVNETSFANLLP
                     TNYTIIARNANQCLSEASSTLTVDETSCLVMNKTVNNASPNIGDNVIFTLSVTNNGPS
                     DATRVVVTDVLPDGYQFVSAPVNYDAINGIWTIGTIAAGASASIQLTASVNTTGNYTN
                     IATITGGDENDLDPSDNTDDATTSPLHPLPVANDDVAYTNENTAITIDVTKNDSFGAP
                     NAPKTGTVVITTHPDASKGTATVNNNGTPTNSTDDKIVFTPKPGYFGDVTMEYEITNY
                     NGDKDRATVTITVSGMRIPDGFSPNGDAINDTFVI"
BASE COUNT         1794 a         1063 c          976 g         1535 t
ORIGIN      
        1 ccaatacttg gatgcgacac accattaaac atggttgttt tgaccattgt ataatgaatc
       61 atatcttcaa acacaatttt atccccaact tttagtggtt gttcaaacga ccactcgcct
      121 acgaaatcgc ccgacagaca actattaccc cccattcgat acgttggttt accttcaata
      181 acatctgtag ctccaacaat acgcggtttg taaggcattt ctaaacaatc gggcatatga
      241 caagcaaacg acacatctag catagccgta gaaacacccc tattctctac aatatccaac
      301 acggtagaaa ccaacacccc cgtttgccac aagaaagcac tccctggctc taaaattaac
      361 tgaatatgag gatacttgct tttgaatgat tttaatagat tgattaaatg ctcggtatcg
      421 taacctttgc gagtcattaa atgtcctcca cccatattca accatttaat ttgagaaaag
      481 aaatgaccaa acttttgttc tacaacttct aaagtagctt ctaagtcaaa cgaacttgat
      541 tcgcataaag tgtgaaaatg caagccttca attccctgag gcaaattatc atccaaatta
      601 gcaacggaaa cacctaagcg ggaaccaact gcacagggat tgtataaatc cgtttcgaca
      661 gaagaatact caggattgat gcgtaagcca cacgaaattg acttgctctt acaaacagca
      721 gcatactgat tatactgtgc tactgaatta aacgtgatat gactactaca cgcagcaata
      781 gcatcaaact cttctggctt atatacgggt gcatacgtat gagctttgct ccccatctct
      841 tcgtaagcca aacgtgcctc actcaaagaa cttgccgttg aatatggaat atattcacga
      901 ataatcggaa ataccttcca cattgcaaaa gctttaaacg caagaataat ttcaacttcc
      961 gcttgctctt taacagactg aataaggagt aaattagcac gcaacaactt ttcgtcaagc
     1021 acaaaacaag gagaaggaat ctgtgatagg ttcatacgga taattttttt tacaaaagta
     1081 cagaaattta gtttatttac gaagtgaaac cgaaaaatac catgtaaaac cgaagtatat
     1141 gctacaaccg ataagtctta actaagtttt cttgtatatt ttattttttt tgtgtttatt
     1201 tgcaaattat taaataatgt gccgtttttt gcatagaaaa cacaaaacta ttacaaaaat
     1261 aaaaaaatat ctaaaccata accacctact ataccagaat actatgagaa agtttacaac
     1321 cacacatcaa ctacgttttg gattaatcgt attgagtttg ttttcttttt tggcttcaca
     1381 cgccatcact atttatttta aagataccaa taattcttgg ggaggagtaa atatacacgt
     1441 atggaacgct actattagct ctgataaaac atcaggatgg cctggaaaag ctatgatttg
     1501 gaaagccact aatggttcag atacttggta taaaattgaa ttacaaagta cgacagcaag
     1561 ctttaaattc aatcgtatag cagataacaa tacgcaaaca tcaaactttg aaaacattac
     1621 aattgatggt tggtatgatt cgggaagtag tggacctcag aatatttcag gcgttccttc
     1681 tggattaaat tttgaattat ccttaggtgc tgtaacgatg tcttctgacg cattagatgg
     1741 ttggggtgta agaacagcca caaccaatgc aggaggagga atttttacat tagtgaaatt
     1801 atatacacaa aatttaggga ttgatatatg gtatggtact agtaagcatg cccatatagc
     1861 aaccactcaa attataggac actcttcatc catggtaggg aaactagcta cttttacgtg
     1921 gaatgccaat accaatgtat tatcaatagc agctggaata acaataacat ccaatctacc
     1981 aagtccgata gatgctggca caaatgtaac gctaacagca agtgcaacca acgagccttc
     2041 taattatgta tgggaatatt caatcaataa cggcggaaca tggcttccat atactggcga
     2101 aaaaacagga gaaaaagaca gatctattac agttactcct tctactacta ctatttaccg
     2161 tgctataatg ggaagtgata atgaaacact cgaattatta gttaaaaaag cttgtgccca
     2221 cgaaacacgt acactattta tagatgattt tggcacatta gttgatcaaa aagatagaac
     2281 gacaagccca tatgttccag ctactaatta cacatacaaa cctagtggaa aagaaattag
     2341 agatagtcac tatgctgttg ttactactcc tcgctttgct gggtgtggaa ctcaaactgg
     2401 agacaatgtt gctggatgta acgttgctgg cgattattgg tatgtagact taaaagatag
     2461 atcgaataga acaactacgc catttggagg aatgctattt gtaaattgca gaaacaagga
     2521 tgcagatgtt gctaaaaatg tggtatatac acgccaaacg attaatttac ctcccaatgt
     2581 tgaacttact ttttcagcat ggtttgccaa tgctcaaaat ccaacgagag gtacaaaggt
     2641 tgatataaat atgcgtttcc aaattttaaa tgcttctaat actgtagtta gtggttcaat
     2701 aattgacgtt cctgccattg ccgcaggtac aggatggttt tatggcgaaa cgaccattaa
     2761 tacaggaagt ggtggattat ttaccgtcca aatattaaat aatggagcat ctggcgttgg
     2821 taatgatgta ctgattgatg atattgaact aaaagtatgt gttcctcaag tatcactgaa
     2881 tgtggatgga caaacgaacg ttaccgttac aggattacat gccacatcac cgttaaccgt
     2941 tccagagagt gtttcttctt tgttttcttc cccttattat ctatgggtag aatacaatcc
     3001 aactactcaa acatgggagg aagtaacagc attttctgga gtaaataaac caacaaataa
     3061 tacctcgttt acatcgtata caccttcgac caaacaattt aaagtgtatg ttgcaagtga
     3121 tagacaatca gctttggatg gatttaattt aggtacaggt ttgtatattg ttacgaatcc
     3181 agttagctta gatttgtgtg acattattac ccagccctct gcaagtggag aagaagtttg
     3241 tgaggggaca ccagcaacag ccttatctgt agtagcaaca agtggtagtg gcacactaag
     3301 ctatcagtgg tatagtaata ctaccgctag caatgtagta ggtggcacct tgataacagg
     3361 agccgaatcg agcaccttta cacctccaac tacatcagcg ggttctacgt attattatgt
     3421 agttgtttct agcaccaatg gttgtagtat gacaagtaac gtatctggag aaattaaagt
     3481 taatccaaaa attatgccta cctttaatgc tgtgggtcca tattgctcag gagatattat
     3541 tcctgccctt tcaacaagtt cgacaaactc gcctgctata acaggaacat ggagtcctgc
     3601 tatttcaaat accgcaaccc aaacatacac ctttacgcca accgctggag cttgtgctaa
     3661 cacaactata ttagaaattg tcgtaaatga aaaaccaaac acaccaagta ttgatgttga
     3721 ttgtacactt ggttttggaa aaggcgtttt gacagttact gctcctttag atgttagctt
     3781 tgaatacgca ctcaacggag gaacttacca agatagtcct acgtttacag gcatagcaaa
     3841 tggaaataat tcaattgtgg ttcgaaataa aactacgcaa tgtacatcaa cagctacaaa
     3901 tttgaatgta gattgtggat gtgcaagtct ccctacagta agttttggaa ctgccaacgg
     3961 tagtacttgt catacaaatg ccataacaat tactggtaat acttttggag gcagtacaac
     4021 cgaagttacc attacgcata atggtagtgg tatactaaca gatgctacat ttacatcttc
     4081 tccatttaac gtaacttaca ctcctgatcc aagtgatgcg gggaaaacaa tagaaattac
     4141 agcgataaca aacaatccat taggtttacc ttgtatagct tcatcagcta tttatgaact
     4201 aactatagag tcattaatta ctcctacgtt tacggctatt gatgcgatat gtcagttaga
     4261 caatttaaat ccactgccca caacatcact caacggtata aatggttctt ggagtcctgc
     4321 attggataat acgcaaacaa ctgaatatac atttacacct gatgcgggtc aatgtgctag
     4381 tgaagtaaaa atgacaatta ttgttcatga taaacctgct caaccaacgg caacagtcac
     4441 acaacctaca tgtgcaacat tacgcggtgc aattgatgtt aataatcata atcatgtgga
     4501 tggacttact tatagcataa atggagtaga ttttgtaaat gaaaccagct tcgccaattt
     4561 gctgcccact aattatacaa tcattgcacg caatgcaaac caatgtctat ctgaggcttc
     4621 aagtacactt accgttgatg aaacaagctg tttggtgatg aataaaaccg ttaacaacgc
     4681 gagtcctaat attggagata atgttatctt tactttatca gttactaata atggtccaag
     4741 cgacgctact agagtagttg ttaccgatgt acttcctgat ggatatcaat ttgtaagcgc
     4801 tcctgtaaac tacgatgcta ttaatggcat ttggactata ggaactattg ctgctggagc
     4861 tagtgcttct atccaactta cagcctcagt aaacacaact ggcaattaca ccaacatagc
     4921 cactattaca ggtggagatg aaaatgatct ggacccatca gacaatacag atgatgccac
     4981 aactagccca ttgcatccac taccagtggc aaatgatgat gtagcatata ctaacgaaaa
     5041 tacggctatt accattgatg ttactaaaaa tgatagcttc ggagctccta atgctccaaa
     5101 aacaggaact gttgttatta caactcaccc tgatgcaagc aaaggaactg caacagttaa
     5161 caataacgga acacctacga actctactga tgataaaatt gtatttaccc caaaaccagg
     5221 ttattttggc gacgtaacaa tggagtacga aattacgaat tacaatggag ataaggatag
     5281 ggcgactgta acgattactg tctctggcat gcgtatcccc gatggttttt cacccaatgg
     5341 cgatgctatc aacgatacgt ttgtgata
//
LOCUS       DAOSRP010000116         5352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_13991_length_5352_cov_4.565226, whole genome shotgun sequence.
ACCESSION   DAOSRP010000116 DAOSRP010000000
VERSION     DAOSRP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_5352_cov_4.565226"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..137)
                     /locus_tag="PLJ82_07280"
     tRNA            complement(62..137)
                     /locus_tag="PLJ82_07280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Lys,seq:ttt)
     gene            complement(359..862)
                     /locus_tag="PLJ82_07285"
     CDS             complement(359..862)
                     /locus_tag="PLJ82_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41342.1"
                     /translation="MKKTVVACLMLLGGITMIVAQTDNRAEMRAAKMQEMQAQKIAFI
                     KEKVSFTDKESAEFFPLYQEFEQQRMELNRKSRPQGKGFKAKNTELSEQEKEAVMDNF
                     IQNRVETANLEKAYYEKFKKILPASKLFEVYQADREFKQELLQDFRDRGAQNQDKKSR
                     ENKSPKK"
     gene            complement(953..1297)
                     /locus_tag="PLJ82_07290"
     CDS             complement(953..1297)
                     /locus_tag="PLJ82_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41343.1"
                     /translation="MEINDNSRNEVGNKVPFTTPDNYFEEFSAKIEQLLDKQEESNQV
                     INLSLWQRVQPYVYLAAMFIGLYVSITTFVKPSIQQKQKEQELVELAIQKELLLDEID
                     DYALYELLSYNN"
     gene            complement(1341..1889)
                     /locus_tag="PLJ82_07295"
     CDS             complement(1341..1889)
                     /locus_tag="PLJ82_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOR41344.1"
                     /translation="MKEIIEESEILAMLASEQTKRKAFSWIMKTYQQKMYYHIRKMLI
                     NHDDTDDVLQNTFLKAWLHIASFRGESSLSTWLYRIATNETLDFLRSKKQTNEYVDIE
                     DTLALSLQDTSYFSGNEIELKLQKAILLLPEKQRLVFNMKYFDDITYEQMSEILETSV
                     GALKASYHLATKKIEKLILLMD"
     gene            2047..2652
                     /locus_tag="PLJ82_07300"
                     /pseudo
     CDS             2047..2652
                     /locus_tag="PLJ82_07300"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
     gene            2681..3265
                     /locus_tag="PLJ82_07305"
     CDS             2681..3265
                     /locus_tag="PLJ82_07305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR41345.1"
                     /translation="MKHPTHISAELMATLPSLSYPEKKIIIVDTLEKLKEAVNKLNTQ
                     KLIGFDTETKPSFTKGVINKVALLQLASEDVCYLFRLHLIGLPHSLMAILANPSIIKI
                     GIALRDDFSGLRKWNNAKPQGFIDIQQEIHKVGIQELSLQKIYAILFARRISKNQRLS
                     NWESEELSESQQHYAAIDAWACLDIYHEIKTYIE"
     gene            3276..4457
                     /locus_tag="PLJ82_07310"
     CDS             3276..4457
                     /locus_tag="PLJ82_07310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOR41346.1"
                     /translation="MYKQIFLKPKKEESLKRFHPWVFSGAISHTDKPVEEGEIVEIYD
                     SAKNFIALGHSQIGSIAVRVLSFHKEEINEIFWVKRIRESASARTSLGVSHSTTHSIY
                     RLVHGEGDLLPGLIVDVYGKTAVMQAHSIGMHVHRHAIAEAIIHVLSPNVSAVYYKSS
                     GTLPYKADIDVEDGFLVGNSTEGTALENGLAFYIDWLKGQKTGFFIDQRDNRSLLEKY
                     ANGKSILNMFCYTGGFSVYALRGGANMVHSVDSSAKAIDLTNKNVTLNFPNDTRHTAF
                     ATDAFKFLAEMDNAYDIIVLDPPAFAKHRDALKNALQGYKRLNAKAIEKIKPGGILFT
                     FSCSQIVSKEQFRLAVFSAAAITGRHVRILHQLTQPADHPISIYHPEGEYLKGLVLMV
                     E"
     gene            complement(4462..5040)
                     /gene="ruvC"
                     /locus_tag="PLJ82_07315"
     CDS             complement(4462..5040)
                     /gene="ruvC"
                     /locus_tag="PLJ82_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOR41347.1"
                     /translation="MSDSLTYKDAIILGIDPGTTIMGYGLLQIKGGKPTMLAIGVLDL
                     KKYPDHYSKLQRIFARVLSLVDEFKPTALAIEAPFFGKNVQSMLKLGRAQGAAIAAAL
                     YRDIPVHEYAPLKIKMAITGSGAASKEQVARMLQRFLNLSEQDMSLQLDATDGLAAAY
                     CHFLQLNKPFSSSKKTSWKAFAQQNSHRVKGL"
     gene            complement(5041..>5352)
                     /locus_tag="PLJ82_07320"
     CDS             complement(5041..>5352)
                     /locus_tag="PLJ82_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOR41348.1"
                     /translation="FSMLAYNITFTMDYNVVNFWEQWIVQKFFPMMLETSLHSPKIFK
                     VYTNATEQRIFTVQFLVESFSDYDSFQKDNEAAIIKQLAKTFGNDVVCFTSFIQEISL
                     E"
BASE COUNT         1741 a          911 c          869 g         1831 t
ORIGIN      
        1 acccaggacc ccatccttaa aagggatgtg ctctacctgc tgagctagcg aatctggctt
       61 ttggtgatcc gcctggggct cgaacccagg accccatcct taaaagggat gtgctctacc
      121 tgctgagcta gcgaatctta tctttttcta caaaagcggt gcaaagataa agttatattt
      181 tttatttcgc aagtaaacaa ctgctttttt agattttgat atcactatat ttatgtccta
      241 ctattgtcaa tcacttaata atgttggatt tatgataaat aaataattaa aaaaaaatgg
      301 aaaaggaaag attttacttc ccttttccat acacacattt tcagaacatt cttgattatt
      361 attttttagg ggatttattc tctcttgatt ttttatcttg gttttgtgct cccctatccc
      421 taaaatcttg tagcaattct tgtttaaatt ctctatctgc ttgatatact tcaaacaatt
      481 ttgatgccgg taagattttt ttaaacttct cataatatgc tttttcaaga ttagctgttt
      541 ctactctatt ttggataaaa ttatccataa cagcctcctt ctcttgctca ctcagctcag
      601 tattttttgc tttaaagcct ttcccctgag gtcttgattt gcgattaagc tccattcttt
      661 gctgttcaaa ctcttggtac aacggaaaaa attcagctga ttctttatcc gtaaaactta
      721 ccttttcttt gataaaagca atcttttgtg cttgcatttc ttgcattttg gctgctctca
      781 tttctgctct attgtctgtt tgggcaacta tcatagttat tccgcccaaa agcattaaac
      841 atgctactac tgtttttttc atattcattt tcattttttg gtttacactg gagatgtatt
      901 ttgtgcacaa atatttctag gttctatatt acgtattatg cgatattcta tactaattat
      961 tatacgataa aagttcatat aaagcataat catcaatttc atctaataat aactctttct
     1021 gaatagcaag ctccactaat tcttgttctt tttgtttttg ttggatggag ggcttgacga
     1081 aggtagtgat agatacatac aatccaataa acatagctgc taaatacaca taaggttgta
     1141 cacgttgcca aaggcttaag tttattactt gattactttc ctcttgctta tctagtaatt
     1201 gttctatctt agcagaaaat tcttcaaaat aattatctgg cgttgtaaat ggaactttat
     1261 tccctacctc atttctactg ttatcattta tttccatact attgttttct gttactttga
     1321 cgattgatat ttcaaatggt ttaatccatt agtaaaatta atttttctat tttttttgta
     1381 gctaaatgat aagaagcttt taaagcccca acagaagtct ctaaaatttc agacatttgt
     1441 tcatatgtta tatcatcgaa atatttcata ttaaatacca aacgttgttt ttctggcaac
     1501 aataaaattg ctttttgtag ttttaattca atttcatttc ccgaaaaata agaagtatct
     1561 tgtagcgata aggctaatgt atcttcaata tcaacgtatt cattcgtctg tttttttgaa
     1621 cgtaaaaaat ccaatgtttc gttggttgct atgcgataaa gccaagttga cagagaagac
     1681 tctcctctaa aagaggctat atgcaaccat gcttttaaaa atgtattttg aagtacatca
     1741 tcggtatcat catgatttat caacattttt cgaatgtgat aatacatttt ttgctggtat
     1801 gttttcatta tccacgaaaa agcttttcgt tttgtttgtt cagaggctaa catggctaaa
     1861 atctctgatt cctcaataat ttctttcatt caaaataaat ttgtgtgtgt ttattcatga
     1921 ataatgcatt ctattttttt attattcgta tctttgtttc ccaaaatatt tataaagagg
     1981 ctactaaaat acgctttttt attggactta taaaaatata aaaggtttaa ttacttatta
     2041 atctttatgc atactactca tttatcaaca caagctatcg aatttattac tgttagttta
     2101 gcctactgta ctgcattaga agacgatatg tctgaacata caacgatgta ctttgacaaa
     2161 ctttcaaaaa ttctaccttt attatatcta aaagcatcac ttgtagacac aacaaacagt
     2221 aacgaagtat acgaggatgt tgagaaattt gttacagaac atgaatatga atttatacaa
     2281 gcaaaaacag cctctttatt tggagcctat gataactatt tagaggtatg agaagaagtt
     2341 gatacaccta tcatgggaag catatctgag gatttagcag acgtatatca ggatataaaa
     2401 gatttagtaa tgaatgctaa agtaggaaat cctgaagtag taagtattgc tgtaaaaata
     2461 tgtatagaaa actttcagca atactggggt caaaagttgc ttagtgcact gaaacagatt
     2521 cacagtattt tatacaataa tgaattagaa aatgtaacag atgagtctat attacaaaaa
     2581 aatacggtaa aaaaacagac ttttagcgat tttttgcgtg atgaagaaga tgactacagc
     2641 aatttattgt aagtttttat ccctaataag tgaatgtaag atgaaacatc cgacacatat
     2701 ttcagccgaa ttaatggcaa cccttccctc actttcttac cccgaaaaaa aaattatcat
     2761 cgttgatacg ctggaaaaat taaaagaagc tgtaaataaa ttaaatactc agaaattaat
     2821 aggttttgac acagaaacga aaccatcctt tacaaaagga gtaatcaata aagtagcatt
     2881 actacaatta gcctctgaag atgtttgtta tttatttcgc ttacacctca ttggattacc
     2941 ccactcttta atggctattt tagctaatcc atctattata aaaattggga tagcattacg
     3001 tgatgatttt agtggattac gcaaatggaa caacgctaaa cctcaaggtt ttattgatat
     3061 tcaacaagag attcataagg tgggtatcca agaacttagt ttgcaaaaaa tatatgccat
     3121 cttatttgct cgtagaattt cgaaaaatca acgactatcc aattgggagt cggaagaatt
     3181 gtcggaatca caacaacact atgcagccat agacgcatgg gcatgcttgg atatttatca
     3241 cgaaattaaa acttatatcg aataaaatag agcttatgta caaacagata tttttaaaac
     3301 caaagaaaga agaatcatta aaacgttttc atccttgggt attttcaggg gctatttctc
     3361 ataccgacaa gcctgttgaa gaaggagaga ttgtagaaat ttatgattca gcgaaaaact
     3421 ttattgcgtt aggtcatagc caaattggaa gcattgcagt tcgtgtactg tcttttcata
     3481 aagaagaaat taatgaaatt ttttgggtaa aacgcattcg tgaaagtgct tctgcacgta
     3541 cctcgttggg tgtttcgcac agcacaactc actcaatata tcgactagtg catggagaag
     3601 gggatttgct tccaggctta atagtagatg tttacggaaa aacagccgta atgcaggctc
     3661 attctatagg aatgcatgta catagacatg ccatagcaga agccattatc catgtattgt
     3721 ctccaaacgt cagtgctgtt tattataaat cgtcaggaac attaccctac aaagctgata
     3781 ttgatgtaga agatggattt ttggttggca attcaacaga aggcactgca ttagaaaacg
     3841 gattagcatt ttatattgat tggctgaaag gtcaaaaaac aggctttttt atcgatcagc
     3901 gagataatcg ctctttgctt gaaaaatacg caaatggcaa atctatttta aacatgtttt
     3961 gctatacagg gggtttttct gtatatgctt taagaggagg tgccaacatg gtacattcgg
     4021 ttgatagttc agctaaggca atagacttaa caaataaaaa cgtaacgcta aatttcccaa
     4081 acgatactcg tcatacagct tttgccacag atgcatttaa gtttctagct gaaatggata
     4141 atgcgtatga tatcattgtt cttgaccccc cagcgtttgc caaacaccga gatgcattaa
     4201 aaaatgcctt acaaggctat aaacgcctaa atgcaaaagc aattgaaaaa attaaaccag
     4261 gaggcatatt gtttactttt tcttgttcac aaattgtgag caaagaacaa tttagattag
     4321 cggtatttag tgcagccgct attacaggta gacatgtgcg tattttacat caattaaccc
     4381 aaccagctga ccaccccatt agcatctatc atcctgaagg agaatatctt aaaggattgg
     4441 ttttgatggt agaatagaca gttataagcc ttttactcta tgtgaatttt gttgagcaaa
     4501 agccttccaa gatgtttttt tagaagaaga aaagggtttg ttgagttgta aaaaatgaca
     4561 ataagctgca gctaaaccat cagttgcgtc taattgtagc gacatatctt gttctgacaa
     4621 gtttaaaaaa cgctgtaaca ttctagcgac ttgctcttta gatgctgctc ccgaacctgt
     4681 tatagccatt tttattttta atggggcata ttcatgtact ggaatgtcgc gatacaaagc
     4741 tgctgctatt gcggctcctt gggctctacc taatttgagc attgattgca catttttacc
     4801 aaaaaaagga gcctctatag ctaatgcagt tggtttaaac tcatccacta aagataatac
     4861 acgagcaaaa atacgttgca atttagaata atgatctggg tattttttta aatctaacac
     4921 accaatagca agcatcgttg gtttaccacc tttaatttgt aacaaaccat accccataat
     4981 agtagtacca gggtcaattc ctaaaataat ggcatcttta tatgttaaac tgtctgacat
     5041 ttactctaat gatatttcct gaataaaaga cgtaaaacat actacatcat taccaaatgt
     5101 ttttgccaat tgtttgatta tcgcagcctc attatctttt tggaaactat cataatcaga
     5161 aaaagactct acaaggaatt gcacggtaaa aatgcgttgt tctgttgcat tggtataaac
     5221 tttgaaaatt tttggtgaat gtaaagacgt ttcaagcatc ataggaaaaa atttttggac
     5281 aatccattgt tcccaaaaat taacaacgtt gtaatccatt gtaaatgtaa tattataggc
     5341 taacatacta aa
//
LOCUS       DAOSRP010000117         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_15493_length_4942_cov_4.476161, whole genome shotgun sequence.
ACCESSION   DAOSRP010000117 DAOSRP010000000
VERSION     DAOSRP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15493_length_4942_cov_4.476161"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PLJ82_07325"
     CDS             complement(<1..1015)
                     /locus_tag="PLJ82_07325"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOR41349.1"
                     /translation="MKRCIMFIFYTSLLCTLFGQTKIHVHRSDAVDIRLFTSLLDSIN
                     FQALPEKPSEYSTLTLHKNNQTTQVVPLQAVDSIEFGVDQSEYIFDITALPEIKLTVK
                     LKDWNALLTYYDINNSNEECVPASIEFKKNGVATQLDSIGIRIRGNSSRRRPEGGKGD
                     MHNATNPSWHHAHFSLKFAEYRKGQSMYSTDRFNLKWFKDDAMYCREIYCYDLFKRFG
                     VWTAPRASYARLTIYVEGDAQPAYFGVYSIIEAVNDDYLKYRVLQGKFTDDNGNLWKA
                     NWGADLSSMDDSKMGVENITLNPSTMQTYVYDLKTNRKNGLAAAKEQLKNFVNEMSRL
                     PSGS"
     gene            complement(1085..2500)
                     /locus_tag="PLJ82_07330"
     CDS             complement(1085..2500)
                     /locus_tag="PLJ82_07330"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOR41350.1"
                     /translation="MKKILLFLALMLLLGCATKPPYVPPGGDIAGSSDYVFDITSLPE
                     ITITVPLEEWNLLLSNYDINRDNEEKVVAHFAFKKNGKVDEIANVGLRLRGNTSRKRP
                     EGYEGQPHNPINPDWRHAHFSVKFDEYVSKRTFYSIDHLILKWFKDDGMYCREIYCYD
                     LFKRFGVWTAPRASYAKLTINIEGGAEPAYFGVYAMIEGVNDSYLDYRKEQGKWASSK
                     GNLWKAKFSQADLSNMDDWRMGISNVTLDPAETVEYVYDLKTNKKTGLDAAKSELKDF
                     INNMNPLKSGSVALKSYLEEHMDVDLFLRAYAVNVMVGMWDDYWMSANNYYFYFDTNG
                     KFYFIPFDYDNTLGTSLYVNAGTHDMLNWGPRDGTRVLMRKVLSIQEYEDRFKEYIKE
                     LADANNDLFYTTKSIARIQSWHAMIEPYIANDTGQDMNLVDVPGSWGNCEFYRILSGN
                     DEGGENGDANFFKTKIKSIYW"
     gene            complement(2564..2959)
                     /locus_tag="PLJ82_07335"
     CDS             complement(2564..2959)
                     /locus_tag="PLJ82_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HOR41351.1"
                     /translation="MVIQQLSIFLENKAGRLNDVAALLGKAGVNMTAFSLADNSDFGI
                     LRVIVSDPHKAQEVLKAQKFAVSLTDVIHLQIANTPGSLSNVLAVLADAGMYIEYMYA
                     FSEGNGANVIIKPDNVPNAVEALQAHKIM"
     gene            complement(2981..4282)
                     /locus_tag="PLJ82_07340"
     CDS             complement(2981..4282)
                     /locus_tag="PLJ82_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOR41352.1"
                     /translation="MIWNEHIECMSREQLRDLQTARLKETVERVYHNTPFYRKKMQEL
                     GILPSDIKDIDDIQKLPFTTKEDLRDNYPFGLMAVPMSEVVRIHASSGTTGKPTVVAY
                     TRKDVSQWTECVSRCFAAFGATKQDIFQISYGYGLFTGGLGAHYGTENIGASVIPMSS
                     GNTQKQLQIMQDFGATVLCCTPSYALFIADKLADMGISKDDLKVRIGVFGAEPWTENM
                     RKELEEKLGIKAYDIYGLSEMCGPGVGYECQNQSGTHLCEDQFYPEIVDPKTLKPVKQ
                     ATEGELVFTHLTKEAMPLLRYRTKDLTSLNYDTCACGRTAVRMGKILGRSDDMLIIRG
                     VNVFPSQIESVILQMPEFEPHYMIYVNRENNLDTMELHVEARPDLYSDNVGTMISLTK
                     KLTAQLNSILGIAVKVKIVEPRSLARSEGKAKRVFDNRMLK"
     gene            4413..4595
                     /locus_tag="PLJ82_07345"
     CDS             4413..4595
                     /locus_tag="PLJ82_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOR41353.1"
                     /translation="MKTNKLTRSDERVIGGVCGGIAEYFDLDVSLIRVVWALVALIGG
                     TGLLAYLIAWLIIPQK"
     gene            4616..>4942
                     /locus_tag="PLJ82_07350"
     CDS             4616..>4942
                     /locus_tag="PLJ82_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOR41354.1"
                     /translation="MKELITKTIDLQSGNPEEKREEIRAYFLKTWALDELLYTQLRDD
                     SIFYHRGDSLRHILLFYLGHTAVFYINKLFLAKIIHQRINPEFESLFAIGVDEMSWDD
                     LNDKNYN"
BASE COUNT         1539 a          995 c          782 g         1626 t
ORIGIN      
        1 cactaccact tggcaaacga gacatttcat taacaaagtt ttttaattgt tctttggctg
       61 cagctaaacc attctttcta tttgttttca agtcatacac atatgtttgc atcgtggatg
      121 gatttaaggt tatattctcc acccccatct tagaatcatc cattgagctt aaatctgctc
      181 cccaatttgc tttccataaa ttaccattat catcggtaaa tttaccttgt aaaactcggt
      241 atttcaaata gtcatcgttt accgcttcaa tgatggaata cacaccaaaa tacgcaggtt
      301 gtgcatcgcc ttctacatag attgtaagtc gggcatagct tgctcgtgga gctgtccata
      361 cgccaaatcg cttaaataaa tcgtagcaat aaatctctcg acaatacatg gcgtcgtctt
      421 taaaccactt taaattaaag cggtcggtac tatacataga ttgcccttta cggtattcgg
      481 caaatttaag agaaaaatgt gcatgatgcc agcttggatt tgtggcatta tgcatatcac
      541 ctttacctcc ttcgggacgt cttcggctcg aattacccct tatacgtata ccaatactgt
      601 ctaattgggt agctacacca tttttcttaa actcgatact agcaggcaca cattcctcat
      661 tactattatt gatatcgtaa taagtgagca aagcattcca atctttcaat ttcacagtca
      721 acttaatctc tggaagagct gttatatcaa aaatatattc actttggtca acaccaaatt
      781 ctatgctgtc tactgcttgc aatggaacaa cttgtgttgt ttggttgttt ttatgtaatg
      841 ttaaagttga atattcagag ggtttttctg gtagtgcttg aaagtttatg ctatcaagta
      901 gcgaagtaaa caatctaata tccacagcat cagaacgatg tacatgaatt ttggtctgac
      961 caaaaagcgt acaaagcaaa cttgtgtaaa aaataaacat aatacatctt ttcatagtaa
     1021 tcagatttta aggttttgaa atattcagcc attttatata tgaaagtact aacacttatc
     1081 tcatttacca ataaatgctt ttaatctttg ttttaaagaa attagcatca ccattctctc
     1141 ctccttcatc atttccagaa agaatacggt aaaattcgca attcccccat gaaccaggta
     1201 catctactaa gttcatatct tgtccagtat cattggcaat atatggttca atcatagcat
     1261 gccacgattg aatacgagca atactttttg ttgtataaaa aaggtcgttg ttagcatcag
     1321 ccaattcttt gatgtactct ttaaaacgat cttcatattc ttgtattgac aacactttac
     1381 gcatcaatac acgcgttcca tctctcggtc cccaatttag catatcatgt gtacctgcat
     1441 ttacatacaa tgatgttcct aaggtattat cataatcaaa cggaataaaa taaaatttgc
     1501 cgttcgtatc aaaataaaaa taatagttat tagcactcat ccaataatca tcccacatac
     1561 cgaccattac atttacggca taagcacgta aaaaaagatc tacatccata tgttcttcta
     1621 aataactttt tagagcgaca ctgccacttt ttaagggatt catgttattg ataaaatctt
     1681 tcaattctga tttagcagca tctaatcctg tttttttatt tgtttttaaa tcatacacat
     1741 attcaacagt ttcagcggga tcaagcgtaa cattgctaat acccatacgc caatcatcca
     1801 tatttgagag gtctgcttga gaaaattttg ctttccacaa gttaccttta ctacttgccc
     1861 atttaccttg ttctttacga taatctaaat aagaatcgtt caccccttca atcatagcgt
     1921 acaccccaaa ataggctggt tctgctcctc cttctatatt gattgttagt tttgcataac
     1981 tggcacgtgg agccgtccat acaccaaaac gtttaaataa atcgtagcaa taaatctcac
     2041 gacaatacat gccatcatct ttaaaccatt ttaaaattaa atggtcgatg ctataaaatg
     2101 tacgtttact aacatattcg tcaaatttaa ctgagaaatg agcatgacgc caatcaggat
     2161 taatcgggtt gtgtggttga ccctcatacc cttcgggtct tttcctactg gtatttcctc
     2221 taagacgtaa accaacgttg gctatttcat ctactttgcc attttttttg aatgcaaaat
     2281 gtgctactac cttttcctca ttatcacggt taatatcata attagatagc aataaattcc
     2341 attcttctaa aggaaccgtt attgttattt caggcaatga ggtaatatca aatacataat
     2401 cactacttcc agcaatatct cctccaggag gtacataggg aggttttgtg gcacatccta
     2461 gtaaaagcat tagagccaaa aaaagaagta tttttttcat gtataagatt aaggtctgta
     2521 aacgaaaaca ataaataaaa agacagatta ttgtctaatt attttacatt attttatgtg
     2581 cttgtaatgc ttcaacagca tttggcacat tatctggttt gattattaca ttagctccat
     2641 ttccttcgga aaaagcatac atatactcaa tatacatacc agcatctgcc aatactgcaa
     2701 gtacattgga taatgagcca ggcgtatttg ctatctgtaa atgaataaca tcggtaagcg
     2761 aaacagcaaa tttttgtgcc ttgagtacct cctgagcctt gtgtggatca gatacaataa
     2821 cacgcaaaat accaaaatca gaattatcag ctaaactaaa tgctgtcata ttaacacctg
     2881 cttttcccaa caaggctgct acatcattta atctacctgc tttattttct aaaaaaattg
     2941 acaattgttg aatgaccata tcattatctg tttttaaagg ttattttaac atccgattat
     3001 caaacacacg ttttgctttt ccttcgctac gtgccaacga gcgcggctcc acaattttca
     3061 cttttacagc tatgcctaaa atactattta attgtgcagt taattttttg gtcaacgaaa
     3121 tcatagtgcc cacattgtcc gaatacaaat cgggacgtgc ttcaacatgc aattccattg
     3181 tatccaaatt attttctctg tttacataaa tcatgtagtg aggttcaaat tcgggcattt
     3241 gcaaaataac cgattcaatt tgagaaggaa acacatttac gcctctaata atcaacatat
     3301 catcgcttct gcctaatatt ttacccatac gcacagctgt acgtccacac gcacaagtat
     3361 cataattcaa cgaagttaaa tctttggtac gataacgcaa caatggcata gcctctttgg
     3421 ttaaatgtgt aaataccaat tcgccctcag tagcttgctt tacaggtttt aaagtcttgg
     3481 gatctacaat ttcaggataa aattggtctt cgcataaatg tgtccccgat tggttttggc
     3541 attcgtaccc aactccaggt ccgcacattt cgctcaatcc atatatgtca taggctttta
     3601 tacccaattt ttcttctaat tctttacgca tgttttctgt ccacggttca gcaccaaaca
     3661 cgcctatgcg tacctttagg tcatctttag aaatacccat atcggctagc ttatcggcaa
     3721 taaacaaggc atacgatggc gtacaacaca gtacggtagc tccaaaatct tgcattattt
     3781 gcaactgttt ttgggtattt ccactcgaca tggggattac agatgctcca atattttcgg
     3841 taccataatg agcacccaaa cctcctgtaa acaatccata gccatacgaa atctgaaaaa
     3901 tatcttgttt ggtggcacca aaagcggcaa aacagcgaga aacacactca gtccattgcg
     3961 acacatcttt gcgtgtgtac gctaccacag taggtttacc tgtagtaccc gaagaggcat
     4021 gaatgcgtac tacctctgac ataggtactg ccattaatcc aaaaggataa ttatcacgta
     4081 aatcttcttt ggtggtaaaa ggtaatttct ggatatcatc aatatcctta atatcagagg
     4141 gtaagatacc caattcttgc attttcttgc gataaaaagg tgtattgtgg tacactcttt
     4201 caactgtttc ttttaggcga gctgtttgta aatctcttaa ctgctcgcga ctcatacatt
     4261 caatgtgttc gttccagatc atatagtatt agattaaaag gcaaatatac atatttaatt
     4321 tatcttaaca caaatatttg aaacaattta tttcttgatt ggtttctaaa agatattgat
     4381 tatttttgca agtaaataat taacgaaacg atatgaaaac aaataagctt acacgttctg
     4441 atgagcgagt tattggtgga gtttgcggtg gaatagccga gtattttgat ttggatgtta
     4501 gtttaattcg cgttgtatgg gctttggtag ctctaatagg tggcacaggg cttctggctt
     4561 accttatcgc ttggctaata atcccccaaa aataaccaat caggagtagt aatacatgaa
     4621 agagttaatc acaaaaacca ttgacctgca atcgggtaat cctgaagaaa aacgcgaaga
     4681 aatacgtgcg tattttttaa aaacctgggc tctcgacgaa ctgttatata cacaattgcg
     4741 tgatgatagt attttttatc acagaggcga ttctttgcgg catattttgc ttttttattt
     4801 ggggcacact gctgtatttt atatcaataa gctgttttta gcaaaaatta ttcaccaacg
     4861 aattaatcca gagtttgaat cattatttgc cataggtgtt gatgaaatga gttgggacga
     4921 cttaaacgat aaaaactata at
//
LOCUS       DAOSRP010000118         4856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_15874_length_4856_cov_4.912935, whole genome shotgun sequence.
ACCESSION   DAOSRP010000118 DAOSRP010000000
VERSION     DAOSRP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15874_length_4856_cov_4.912935"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLJ82_07355"
     CDS             complement(<1..258)
                     /locus_tag="PLJ82_07355"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOR41355.1"
                     /translation="MVKQQYITLYQQYAHIIKEKSAPLLNSIRDAAFKRFEELGFPVK
                     RDKDYEYIDIQSSYDHDFGLNLNRVSLDVDPYKVFTCDVPNI"
     gene            complement(260..1012)
                     /gene="sufC"
                     /locus_tag="PLJ82_07360"
     CDS             complement(260..1012)
                     /gene="sufC"
                     /locus_tag="PLJ82_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOR41356.1"
                     /translation="MLVIKDLHANVNGKEILSGINLTVNPGEIHAIMGPNGSGKSTLS
                     AVLAGNPVFEVTKGEVYFQGKNLLEMAPEDRAREGVFLSFQYPVEIPGVSMVNFMRTA
                     INEHRKYNNLEPISASDFLKLMREKKEIVELDTKLTNRSVNEGFSGGEKKRNEIFQMA
                     MLNPKLSILDETDSGLDIDALRIVANGVNQLKNSDNASIVITHYQRLLEYIVPDFVHI
                     LYKGRIVKTAGKELALILEEKGYDWIKQEIEE"
     gene            complement(1060..2499)
                     /gene="sufB"
                     /locus_tag="PLJ82_07365"
     CDS             complement(1060..2499)
                     /gene="sufB"
                     /locus_tag="PLJ82_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOR41357.1"
                     /translation="MDEKNIIEDVTSSEYKYGFTTEIETDVIPIGLNEDVVRIISAKK
                     NEPDWMLDFRLKAYRYWITQKMPSWANLSLPEIDYQAISYYAAPRSTMPQSIDDVDPE
                     VIKTFDKLGIPLHERDKLLGIAVDAVMDSVSVKTTYKESLAELGIIFCSFSEAVTNYP
                     DLVKKYLGSVVPYTDNFFAALNCAVFSDGSFVYIPKGVRCPMELSTYFRINAANTGQF
                     ERTLIVAEEDSYVSYLEGCTAPMRDENQLHAAIVEIVILDRAEVKYSTVQNWYPGDKN
                     GVGGIYNFVTKRAICKGDSAKVCWTQVETGSAITWKYPSCILKGDNSIGEFYSVAVTN
                     NYQQADTGAKMIHLGKNTRSRIVSKGISAGKSQNSYRGMVKVQKGAENARNFSQCDSL
                     LLSDRCGAHTFPYIEVNNDTAIVEHEATTSKISEDQIFYCNQRGINTEDAVGLIVNGF
                     AKEVLNKLPMEFAVEAQKLLQISLEGSVG"
     gene            complement(2502..3002)
                     /locus_tag="PLJ82_07370"
     CDS             complement(2502..3002)
                     /locus_tag="PLJ82_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOR41358.1"
                     /translation="MENYIDIVLIIPLIWGLIRGLFKGFIMTVGSIIGFILGFYVSNN
                     YALSLSSYLQSWFSLSTQIAYVFAYLMIFLVVILSIFSISLLLEKTIKHISLGWLNKV
                     IGAIVGCLKYALIVSLLLNLVEIIDEKVDIVAEETKQSSYMYKPLLQIVPAILPSITY
                     YEDKKL"
     gene            complement(3093..>4856)
                     /gene="infB"
                     /locus_tag="PLJ82_07375"
     CDS             complement(3093..>4856)
                     /gene="infB"
                     /locus_tag="PLJ82_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR41359.1"
                     /translation="ERLESRVIKITEFVTANALATMMDVPVTQVIGTCMSIGIMVSIN
                     QRLDAETINIVAEEFGFKTEYVSAEVAQAIHVEEDAEEDLLPRAPIVTVMGHVDHGKT
                     SLLDYVRRANVIAGEAGGITQHIGAYNVTLDSGQKITFLDTPGHEAFTAMRARGAKVT
                     DIAIIIVAADDDVMPQTIEAINHASAANVPMVFAINKVDKPNANPEKIKEALAGMNYL
                     VEEWGGKYQSHDISAKQGMGVKELLEKVLLEAELLDLKANPNKKAIGSIIESSLDKGR
                     GYVSTVLVQTGTLKMGDVIIAGTYFGKIKAMFNERNQRINEAKPSEPVLILGLNGAPQ
                     AGDEFHVLETEQEARDIANKREQLQREQSIRTKKHLTLDELGRRIALGNFQELNVIVK
                     GDVDGSIEALSDSLIKLSTPEIQVNVIHKAVGAISESDVMLATASNAIVVGFQVRPSI
                     AARRLAENEGIDIRLYSIIYDAIEEIKSAMEGMLSPEIKEVVTATLEIMEVFKITKVG
                     NVAGCLVREGKIKRTNKIRLIRDGIVIYSGQLGSLKRMKEDVKEVALGYECGLNIGNY
                     NDLRVGDIIESFEEVEVKKTL"
BASE COUNT         1502 a          993 c          702 g         1659 t
ORIGIN      
        1 tatattggga acatcgcaag taaacacttt atatggatca acatctaaac tgactctatt
       61 tagatttaat ccaaaatcat gatcgtatga agactgaata tcaatgtatt cgtaatcttt
      121 atctcgtttg acgggaaaac ccagttcttc gaaacgctta aaagcagcat cacgaatact
      181 attcaacaat ggagcacttt tttctttaat gatatgtgcg tattgttggt ataacgttat
      241 atattgttgc tttaccatac tattcctcaa tttcttgttt aatccaatcg tatccttttt
      301 cttcgaggat aagagctaac tccttgccgg ccgtttttac aattcgacct ttgtataaaa
      361 tatgaacaaa atcggggacg atatattcaa gtaatcgttg ataatgagta atcacaatgg
      421 aagcattatc gctatttttc aattgattaa ctccattagc tacaatacgt aaggcatcta
      481 tatctaatcc agaatccgtt tcatctaaaa tagacaattt tggatttagc atagccattt
      541 gaaaaatttc attccttttc ttttcgccac cagagaaacc ttcatttacc gaacgattcg
      601 ttaatttggt atcaagttct actatttcct ttttttctcg catcaatttt aagaaatcgc
      661 ttgcagaaat tggttcaagg ttattgtatt tacgatgttc gttgatagca gtacgcataa
      721 aattaaccat actaacacca ggtatttcta ctgggtattg aaaactaaga aaaacccctt
      781 ctcttgcacg gtcttcggga gccatttcga gcaggttttt cccttggaaa taaacctctc
      841 cttttgttac ttcaaatact ggatttcctg ctaaaacggc agataaagta ctttttcctg
      901 aaccgttcgg acccataata gcatgaattt ctcctggatt aacagttaaa tttattcccg
      961 acaatatttc tttgccattt acgttggcat gtaaatcttt tattactagc atattgatat
     1021 tatttataaa atttataaag tataagatta tattaaaatt tatcctacac ttccttctaa
     1081 agatatttgc aataattttt gagcttcaac ggcaaactcc ataggtaatt tatttaacac
     1141 ctctttggca aatccgttga caatcagacc aaccgcatct tcggtattga taccacgttg
     1201 attgcaataa aatatttggt cttcgctaat ttttgatgtt gtagcttcgt gttcaacaat
     1261 cgctgtatca ttatttacct caatataggg aaatgtatgt gctccacatc tatcgctaag
     1321 caataagcta tcgcattgcg aaaaattacg tgcattttct gccccttttt gcacttttac
     1381 cattcctcta tacgagtttt gactttttcc tgcagatatt cctttagaca caatacgact
     1441 tctggtattt ttgcctagat gtatcatttt ggcaccggta tccgcttgtt ggtaattgtt
     1501 agttacagct acagaataga attctccgat agaattatct cctttaagaa tacaacttgg
     1561 gtatttccac gtaattgcag aacctgtttc cacttgggtc caacaaactt tagcactatc
     1621 tcctttgcaa atagcacgtt tcgtaacaaa attatatata ccacctacgc catttttatc
     1681 acctggatac caattttgga ctgttgaata ttttacttca gccctatcta atatcacaat
     1741 ctctacgatg gctgcatgca gttgattttc atctctcatt ggagctgtac atccctctag
     1801 ataagaaacg tacgaatctt cttctgcaac aataagggta cgttcaaatt gacctgtatt
     1861 agctgcatta attctaaaat aggttgataa ctccatggga caacgcactc cttttggtat
     1921 atacacaaaa gagccatcac taaaaacggc acaattaaga gcagcaaaga aattatctgt
     1981 ataaggtact acagagccta ggtatttttt caccaaatct ggataattgg taacagcttc
     2041 gctaaaagag caaaaaataa tacccaactc agctaaactc tccttatagg tagtttttac
     2101 agatacactg tccattactg catccacagc aattccaagt agcttatccc tctcatgtaa
     2161 cggaatgccg agtttatcaa acgtttttat tacttcggga tctacatcat caatactttg
     2221 gggcatggta gaacgtggtg cagcataata ggaaattgct tgataatcaa tttctggcaa
     2281 ggataaattt gcccagcttg gcattttttg tgttatccaa taacgatacg cttttagtct
     2341 gaaatccaac atccagtcgg gttcattctt tttggcagaa ataattcgta ctacatcttc
     2401 gtttaagcca ataggaataa catctgtttc aatttctgta gtgaatccat atttatattc
     2461 actagatgtt acgtcttcaa ttatattttt ttcgtccatt gttataattt tttatcttcg
     2521 taataggtaa ttgaaggcaa tattgcaggt acaatctgta ataaaggttt gtacatatat
     2581 gaagactgct tagtttcttc tgctacaata tcgacctttt catctataat ttcaactaaa
     2641 ttaagtaaaa ggcttactat aagtgcatat ttcaagcacc ctactatcgc tcctattact
     2701 ttatttaacc aaccaagcga aatatgtttg attgtttttt cgagcaataa agatatggaa
     2761 aaaattgaga gaataactac taaaaaaatc ataagatatg caaaaacata tgcaatctga
     2821 gtagacagag aaaaccaaga ttgcaaatat gagcttaaag acaaagcata gttgtttgac
     2881 acataaaaac ctagtataaa accaattata gagccaacgg tcattataaa acccttaaat
     2941 aaacctcgaa ttaaccccca aatcaacgga ataatcaata caatatctat gtaattttcc
     3001 atcttaaata atattaaaaa aaatctgcat ttacattaat ccagaaaaaa aatcagtttt
     3061 aatgtgaatg cagataataa taataaaaaa tattacaatg tttttttaac ttctacttct
     3121 tcaaacgatt ctataatatc tcctacacgt aaatcattgt aattaccaat attcaatcca
     3181 cactcatatc ctaatgccac ttcttttaca tcttctttca tccgtttcaa agaacctaat
     3241 tgacctgaat atatcactat accatcacga attaggcgga ttttgtttgt tcttttgatt
     3301 tttccttcac gaactaaaca ccctgccaca ttccctactt tcgtaatttt aaacacctcc
     3361 attatttcca acgtagcagt tacaacttcc ttgatctcag gcgacagcat accctccatt
     3421 gccgatttaa tttcttcaat agcatcgtat ataatagaat ataaacgaat gtcaattcct
     3481 tcattttctg ctaaacgacg agcagcaatt gatggtctta cttggaaacc aacaacaata
     3541 gcgttggatg ctgtagccaa cataacatct gattcggaaa ttgcacctac tgctttatgt
     3601 ataacattta cctgaatttc aggagtagat aatttaatta acgaatcgga aagagcttcg
     3661 atagagccat ccacatctcc ttttacaata acatttaatt cttggaaatt gcctaatgca
     3721 atacgtcttc caagttcatc aagcgtaaga tgctttttag tacgtatact ttgctcgcgt
     3781 tgcaattgct ctcttttgtt ggcaatatca cgagcctcct gttctgtttc taatacatga
     3841 aattcatccc ctgcttgtgg tgcaccattt aatccaagta ttaaaactgg ttcagaaggt
     3901 ttagcttcat ttattcgttg gttcctctcg ttaaacattg ccttaatttt accaaaataa
     3961 gtaccagcaa ttatcacatc acccattttg agtgtgcctg tttgtacgag cactgtagag
     4021 acataccctc tacctttatc caaagatgat tcgataattg aacctattgc ttttttattt
     4081 gggttcgctt ttaagtctaa taattctgct tcgagcaata ctttttcgag caattctttt
     4141 acccccatcc cttgtttggc agatatatca tgcgattgat atttacctcc ccattcctct
     4201 acaaggtaat tcataccagc tagggcttct ttaatttttt cagggttagc atttggttta
     4261 tctactttat ttatagcaaa aaccataggt acattggcgg cagaagcatg attaattgct
     4321 tcaatagttt gaggcataac atcatcatca gcagctacaa taataatagc aatatctgtt
     4381 actttagcac ctctagcacg catagctgta aaagcttcgt gaccaggagt gtctaagaat
     4441 gtaatttttt gtccactatc aagagttaca ttatatgccc caatatgttg ggtaatacct
     4501 ccagcctctc ctgcaatcac attagctcga cgcacatagt ctaacaaaga tgttttacca
     4561 tggtctacgt gacccataac agttacaatt ggcgcacgtg gtaataaatc ctcttctgca
     4621 tcctcttcta catgaattgc ttgtgcaact tcggcactca catattctgt tttaaatcca
     4681 aattcttcag ccactatatt aatagtttca gcatcaagac gttgattgat tgaaaccata
     4741 attccaatac tcatacaagt accaataacc tgtgttacag gcacatccat cattgtcgcc
     4801 aacgcattgg ctgtaacaaa ctctgtgatt tttattacgc gactttctaa tctttc
//
LOCUS       DAOSRP010000119         4844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_15933_length_4844_cov_4.872207, whole genome shotgun sequence.
ACCESSION   DAOSRP010000119 DAOSRP010000000
VERSION     DAOSRP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15933_length_4844_cov_4.872207"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLJ82_07380"
     CDS             <1..358
                     /locus_tag="PLJ82_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOR41360.1"
                     /translation="DYDDWTTIGENGLAGLNGDILVWDSVLQLPLELSSMGIRVDKDA
                     LIKQLAITHTEERLQFYYHKRLVEGSLPLSIGGGIGQSRLCMFMLRKAHVGEIQASIW
                     PDSMRKECKELGIFLL"
     gene            376..1053
                     /gene="mtgA"
                     /locus_tag="PLJ82_07385"
     CDS             376..1053
                     /gene="mtgA"
                     /locus_tag="PLJ82_07385"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOR41361.1"
                     /translation="MKVVRILRNIVLGLFLSSILLVILYRFVPVYITPLMIIRVAEQV
                     ADGKPIRLERGWVPIDEISPKMVRAVIASEDNLFLDHYGFDIEGMKKAFENNKKGEKL
                     KGGSTISQQTAKNVFLTPSRTYVRKALEAYFTVLIEIFWSKERIMEVYLNVIEMGNGI
                     YGIEKASEIYFNKPPQKLTSFQAALVAASLPNPRKYSITHPGPYMRKRQAHILWLMPK
                     LGKIEFE"
     gene            complement(1064..1645)
                     /locus_tag="PLJ82_07390"
     CDS             complement(1064..1645)
                     /locus_tag="PLJ82_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41362.1"
                     /translation="MKKVWMYIVLSTVLFTGCIADKRNLQFVESLVETELSNDALQLR
                     YRAPKGFHLVDQHYTDSIANVQLLQDGFSPVVLSIHVDTLKGASMMLSDIRYLPEDKL
                     EKEIKNYKKTYNQHNTWNKVYRMTYSHHNFDIVEINCENDTYLLTKLFFYEDKHPTFV
                     VDYFIPIVEKETYTPLINSSIASFEKGYTLIFH"
     gene            complement(1775..4240)
                     /locus_tag="PLJ82_07395"
     CDS             complement(1775..4240)
                     /locus_tag="PLJ82_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOR41363.1"
                     /translation="MNYTIEVIANIIQANYDKEDSKHIRYILTDSRTVYYPSESLFFA
                     LETSTNDGHKYIEELVQKGVQYFVVHKENPSWKHLHATFLVVKNTLDALQQLAAFHRV
                     VYNIPIVGITGSNGKTIVKEWLHQLLQSDYHLTRSPKSYNSQVGVPLSICLLDETTEI
                     GIFEAGISQPHEMQKLQHIIQPNIGIFTTLGQAHQENFSSMEEKLREKLYLFSSSKVL
                     ICKYNHAIEEILRPINPTISYVSWSFSIDDNPTILIQSVIKKHTESSIRFCYQQQEFS
                     YTIPFVDDASIENSIHCLAFCLWLSVPIYTIIDRMKRLEPIAMRLEKKQGTNNCLLIN
                     DSYNSDINSLHIALDLVDYSAENTYDKRTVILSDIIQSGEEEHILYSKVAKAIQQKNI
                     SRFIGIGKHISQYAHLFEGKTYFYHTTKEFLFSNIIDSFHNEAILVKGARSFEFEKII
                     NKLELKMHETVLEINLNALVNNFNYLRSYLLPTTKTMCMIKANAYGCGAIEVAKTLQY
                     HRCDYFGVAVADEGVELRKAGIHTPIIVMNPEPSSFDLLCEYRLEPEIYSFKLLKAFA
                     EHLNKYGIINYPIHIKIDTGMKRLGFEPTDMNTLIHFIKAQTSCQVQSIFTHLVGADD
                     AIFDDFTQHQLKLFTQCCDVLQSHCNHPIIRHALNSAGVERFPNFQFDMVRLGIGLYG
                     IATSNQHAIQPLVTLKTIVLQVKNIAAHETVGYSRKGSLHRNSRIATIPIGYADGLKR
                     NLGNGNGYVLIDGKKAPFVGNICMDISMVDITDIPHVEEGDTVVVIGNQIHINEIAEK
                     AGTIPYEILTGISQRVKRIYFQE"
     gene            complement(4278..>4844)
                     /locus_tag="PLJ82_07400"
     CDS             complement(4278..>4844)
                     /locus_tag="PLJ82_07400"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HOR41364.1"
                     /translation="PLVLALYNLIVRKKAGFIAILGFISIFVTGIIGVFEFPSEWIAY
                     KEASVPLIIGIMVLISLKTPFPLVKKFLYNEEILNVEKIDMILVETNNKQAFEKTLVR
                     GTYLLAASFLVSAILNFALAKILIQSPSGTAEFNAELGKMTALSFPVIALPSTIIMLV
                     ALFYIMARLKKLTHLEVEELLSEKMHNK"
BASE COUNT         1490 a          776 c          884 g         1694 t
ORIGIN      
        1 agactacgat gattggacta ccataggcga aaatgggtta gcaggattaa atggtgatat
       61 tttggtgtgg gactctgttt tgcaactgcc tttagaatta tcatctatgg gtatccgcgt
      121 agataaagat gcgttgatca aacaattagc tataacacat acagaagaac gtttacaatt
      181 ttattaccac aaacgcttag tagaaggttc attacccttg tctattggtg gtggtatagg
      241 tcagtctcgc ttgtgtatgt ttatgttacg taaggctcac gtaggtgaga tacaagcaag
      301 tatttggccc gatagtatgc gtaaagaatg taaagagtta ggtatctttt tgctataaga
      361 aatttaccaa tttgaatgaa agtagtgcgt attcttcgta acatcgtact tggattattt
      421 ttatccagta tcttgctcgt tatattgtat cgttttgtgc ctgtttatat tactccttta
      481 atgattatta gggtggcaga acaagtggct gatggtaaac ccattcgatt agaaagaggc
      541 tgggtgccaa tagacgaaat ttcacccaaa atggtacgtg ctgttattgc ctcagaagat
      601 aatctttttt tagaccatta cggttttgat atcgaaggaa tgaaaaaagc ctttgaaaat
      661 aacaagaagg gggaaaaatt aaaagggggg agcactattt cacaacaaac agccaaaaat
      721 gtttttttaa ccccgagtcg tacgtatgtt cgtaaagctt tggaagctta ttttacggtt
      781 ttgattgaga ttttttggtc caaagagcgt attatggagg tgtatctcaa tgtgattgaa
      841 atgggaaatg gtatttacgg tatagaaaaa gcctcagaaa tttattttaa taaaccgcct
      901 cagaaattaa cttcttttca agccgcattg gttgcagcat cgttgcctaa tccacgtaag
      961 tatagcataa cacatccagg tccttatatg cgtaaaagac aagcacatat actatggtta
     1021 atgcccaagt taggaaaaat tgagtttgaa taacacttag gtattaatga aagataagtg
     1081 tatatccttt ttcaaacgat gcaatggatg aatttataag tggggtatat gtttcttttt
     1141 ctacgattgg aataaaataa tcaacaacaa aagtcggatg cttatcttcg taaaagaata
     1201 attttgtgag caagtaggta tcgttttcgc agtttatttc tactatatca aaattatgat
     1261 ggctatacgt catgcgatat actttattcc aagtattgtg ttgattgtat gtttttttgt
     1321 agtttttaat ttctttctcc aatttatctt cgggtagata tcgaatatcc gaaagcatca
     1381 tagaagctcc ttttagagtg tctacatgta tactcaaaac aacgggagaa aaaccatctt
     1441 gtagcaattg tacattggca atagaatctg tataatgttg gtcaactaaa tgaaaaccct
     1501 ttggagcacg atagcgaagc tgtaatgcgt cgttagataa ttctgtttct accaacgatt
     1561 ccacaaattg taggttgcgt ttatctgcta tgcaacctgt aaacaacacg gtactcaaaa
     1621 caatatacat ccacactttt ttcatctgtt attcagttat tatctagtta tttttcttac
     1681 acagagtttt atactacatt ctctgcaatt ttgttctgtt atttcttttg tgctttttgt
     1741 gcacaacgct tacacagcaa atcctttcat cttattactc ttgaaaatat atacgtttta
     1801 ctcgttgaga tatacctgtt aatatttcat aaggaatagt gcctgctttt tctgctattt
     1861 cattgatatg tatttgattt ccaatcacca caaccgtatc tccctcttct acatgtggta
     1921 tatctgtaat atctaccata ctgatatcca tacaaatatt gcctacaaaa ggagcttttt
     1981 taccgtcaat gagtacatat ccatttccat ttcctaggtt acgttttagc ccatcagcat
     2041 atccaattgg gatggttgcg atacgggagt ttcggtgcaa gctacctttt ctgctataac
     2101 caactgtttc gtgtgcagct atgtttttta cttgcaatac gatagttttt agggttacta
     2161 aaggctgaat agcatgttgg ttggatgttg caattccata caaacctatt cccaaacgaa
     2221 ccatatcgaa ctgaaagtta ggaaatcttt ctactccagc actatttagt gcatgacgta
     2281 taatggggtg gttacaatgg gattgtagta catcacaaca ttgggtaaaa agttttagtt
     2341 gatgttgcgt aaaatcatca aatatagcat catcagcccc taccaaatgc gtgaaaatag
     2401 actgtacctg acacgatgtt tgtgctttga taaaatgaat taatgtattc atatcggtag
     2461 gctcaaaacc caatcgtttc atgccagtat ctattttgat atgaataggg tagttgataa
     2521 taccatattt attaaggtgt tcggcaaaag ctttcagtaa tttaaaacta tatatttcgg
     2581 gttctaaacg gtattcacac agcaaatcga acgagcttgg ttctggattc attacaataa
     2641 taggagtatg gatacccgct ttgcgtaatt caacaccttc atctgctaca gctaccccaa
     2701 aataatcaca tcggtggtat tgtagtgttt tagccacttc tattgcacca caaccatatg
     2761 cattggcttt tatcatacac attgttttgg tagtgggtaa taggtaagag cgtaaataat
     2821 taaagttgtt taccagtgca ttgaggttaa tttctagcac cgtttcgtgc attttaagtt
     2881 ctaatttgtt gatgattttt tcaaattcaa aagatcgagc tccttttaca agaatggctt
     2941 cgttatgaaa actgtcaatt atattggaaa ataaaaactc ttttgttgtg tgataaaaat
     3001 aggtttttcc ttcaaataaa tgagcatatt gggatatgtg ttttccaatg ccaataaaac
     3061 gagaaatatt tttttgttgt attgctttag ctactttact atacaatatg tgttcttctt
     3121 cgcctgattg aatgatgtct gacagaatga cggtgcgttt atcgtaggtg ttttccgctg
     3181 aataatcgac caaatctaat gctatatgca aggagttaat gtccgaatta tagctatcgt
     3241 tgattagtaa acaattgttt gttccttgtt ttttttcaag tcgcatagca atgggttcta
     3301 accgtttcat tctatctatg atagtgtata taggaacaga aagccatagg cagaaagcta
     3361 aacagtgtat actattttca atagatgcat catctacaaa tggtatggtg tacgaaaatt
     3421 cttgttgttg atagcaaaaa cgaatactac tttctgtatg tttttttata acagattgaa
     3481 tgagtatcgt tgggttgtca tctatagaaa atgaccaaga aacatatgaa atagttggat
     3541 taattgggcg taatatttct tcaatagcat ggttatattt acagattaat acctttgatg
     3601 aagaaaataa atatagtttt tcgcgtaatt tttcttccat agaggaaaaa ttttcttggt
     3661 gagcttgtcc aagagtggta aatataccaa tgttaggttg gataatgtgt tgcagttttt
     3721 gcatttcgtg tggttgagaa atgcccgctt caaaaatacc tatttccgtt gtttcatcaa
     3781 gtaaacagat agataaaggt actcctactt gcgaattata gcttttagga gagcgtgtta
     3841 ggtggtaatc tgattgcaag agttgatgga gccattcttt tacaattgtt ttcccattgc
     3901 taccagtaat acctacgatt ggaatattgt atactacacg atgaaaagca gctaattgtt
     3961 gtaaggcatc tagtgtattt ttaacaacta aaaatgtggc atgtagatgt ttccacgatg
     4021 gattttcttt atgtacaaca aaatattgaa cgcctttttg cactaattct tcaatgtatt
     4081 tgtgtccatc gttggtgctt gtttctaagg cgaaaaataa actttcactt ggataataca
     4141 ccgttcgact gtcggttaaa atatagcgaa tgtgtttgct atcttctttg tcatagttag
     4201 cttggataat gtttgctatt acttcaatcg tatagttcat cgttgtatgt tattaaataa
     4261 aaaggaagaa agaatttcta tttattgtgc attttttccg acaataactc ttctacttcc
     4321 aaatgtgtca atttttttaa tctagccata atataaaata aggctactaa cataataata
     4381 gtagagggta atgcaattac tggaaaacta agagctgtca tttttcctaa ttctgcattg
     4441 aattcagccg ttccggatgg actttgaatc aatattttag ccaatgcaaa gtttaagatt
     4501 gctgatacta aaaacgaagc agctaataaa tatgttcctc taaccaaggt tttttcaaat
     4561 gcttgtttat tgtttgtttc tactaaaatc atatcaatct tttctacgtt gaggatttct
     4621 tcattgtata aaaatttttt taccaaaggg aagggcgttt ttaatgaaat aagcaccatg
     4681 ataccaataa ttagtggaac agaggcttct ttataggcta tccattctga tggaaactcg
     4741 aacacaccaa tgatgccagt aacaaaaata ctaataaacc caagaatagc aataaagcca
     4801 gctttttttc ttacaattag gttgtacaat gctaatacta aagg
//
LOCUS       DAOSRP010000120         4816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_16048_length_4816_cov_5.825457, whole genome shotgun sequence.
ACCESSION   DAOSRP010000120 DAOSRP010000000
VERSION     DAOSRP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16048_length_4816_cov_5.825457"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2433
                     /locus_tag="PLJ82_07405"
     CDS             <1..2433
                     /locus_tag="PLJ82_07405"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR41365.1"
                     /translation="QDNWRSNGTDKTFYSSAFTESFCARKQQTSFIPIDSPKTVRFSI
                     HVPRIDSSLCLAIVGNQPALGNWDNKKALVMQDTHYPEWTAEMDASNVNNPIEYKYVL
                     YNRTKKTIVSWEKGANRTLEIDAVIANQLVLKNDETFRYDIPNFKAAGIALPIFSLRT
                     ENSFGIGEFADLKPLVDWSAKTGQKVIQTLPINDTTRSRTNADSYPYSSITVLALHPA
                     YINLIGIGKLKSKKEMDKFAQIQESLKQFKTVKYQEVIEAKWEYFRLIYKQEKEKTFS
                     SADYKQFFAENKQWLLPYAAFCYLRDKHSDSNFRNWKNFSVFDWDKIDDFFKPEFADF
                     DEAMIHIFLQYHAHKQLLEASNYAKSKGIVLKGDIPIGISPDSVEAWMEPHLFNLDSQ
                     AGAPPDPFSATGQNWGFPTYNWERMEKDGYQWWKTRFQKMAAYFQAYRIDHVLGFFRI
                     WRMSADDVQGLLGHFDPALPLTADEIKGFGTWFDYDRMVKPYIREHVLNGLFGDYTNE
                     VKERFLDIEQYGVYRFKKAYNTQKKIEAFFIKNQDKMEEHKIAILQNGLFALFCEVLF
                     IEDSKKQGTFHPRIAFHSTYSYRDLDNGAKNALNNLYNHFYYQKHNDFWKEQAMKKLP
                     ALLQATNMLVCGEDLGMIPECVPSVMHDLYIMSLEIERMPKDPKEEFIHMPNVPYLSV
                     CTTSTHDMNPIRGWWEEDRGTTQRYFNNILNEWGEAPQFCEPWVCEKIVARHLHSPAM
                     LVVLPFQDWLSINGKYRRENILEERINVPSDPKHFWCYRMHFTIEELLENDEVNDKIL
                     ALTQQAGRNSTM"
     gene            complement(2507..4264)
                     /gene="aspS"
                     /locus_tag="PLJ82_07410"
     CDS             complement(2507..4264)
                     /gene="aspS"
                     /locus_tag="PLJ82_07410"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR41366.1"
                     /translation="MYRTHTCGELRLSHIGQQVTLAGWVQRVRRMGGMIFVDLRDRFG
                     ITQLVFNQETNADLCAQANELGREFVIQVQADVAERSNKNKHIPTGEIELLVSTMRVL
                     NTSETPPFTIEDNTDGGDDLRMKYRYLDLRRNTVRENMILRHKVAFETRRYLDQMGFL
                     EVETPILIASTPEGARDFVVPSRMNQGQFYALPQSPQLFKQLLMVSGFDKYFQIAKCF
                     RDEDLRADRQPEFTQIDCEMSFVKQEDVLNLFEGMMKHLFRFVKNIDMTDAFPRMTWA
                     DAMKYYGSDKPDIRFDMQFVEIKELVSGKNFVVFDEADYVGAICASNCATYTRKQLDE
                     LTDFVKRPQIGAKGLVYIRCEADGTFKSSVDKFYTNDDLAKIATACKAEKGDLILILA
                     GATEKTQKALCELRLEMGTRLGLRDKNVFAPLWVIDFPLFQWDEETQRFYAMHHPFTS
                     PNEDDIALLDTNPGKVRATAYDMVINGVELGGGSIRIHESALQKKMFELLGFTPEQAE
                     AQFGFLMSAFKYGAPPHGGLAFGLDRLVSLFAGLDSIRDCIAFPKNNSGRDVMIDAPS
                     VIDNAQLDELGLRIELKKS"
     gene            4442..4516
                     /locus_tag="PLJ82_07415"
     tRNA            4442..4516
                     /locus_tag="PLJ82_07415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4477..4479,aa:Pro,seq:tgg)
BASE COUNT         1567 a          989 c          834 g         1426 t
ORIGIN      
        1 caagataatt ggcgttcgaa tggcaccgat aaaacctttt attcctctgc ttttacggaa
       61 agtttttgtg ctcgtaaaca acagacatct tttattccta tcgattcgcc caaaacagtt
      121 cgattctcta tccatgtacc tcgtatagac tcctcgcttt gccttgctat tgtgggcaat
      181 caacctgctt taggtaattg ggacaacaaa aaagccttag taatgcaaga tacccattac
      241 cctgaatgga cagcagaaat ggatgcgtca aacgtaaaca acccaattga atataaatac
      301 gtgctatata atcgcacaaa aaaaacaatc gtatcatggg aaaaaggtgc caatagaaca
      361 ttggaaattg acgctgtaat tgctaatcaa ttagtactaa aaaatgacga aacatttcgt
      421 tacgatatac ccaactttaa agcggctggt atcgcactac ccattttctc gttacgcaca
      481 gaaaatagct ttggaattgg cgaatttgct gatttaaaac cccttgtcga ttggtcagct
      541 aaaacaggac aaaaagtcat tcaaacgctc ccaattaatg atacaacacg ttcgcgtacc
      601 aatgctgatt cgtatccata tagtagtatt acggtattgg ctttacatcc tgcttacatc
      661 aaccttatag gtattggcaa gctgaaaagc aaaaaagaaa tggataagtt tgcacaaata
      721 caagaatctt taaagcaatt taaaaccgtt aaataccaag aagttataga agctaaatgg
      781 gaatattttc gtttaatata caaacaagaa aaagaaaaaa catttagctc agctgactat
      841 aaacaatttt ttgccgaaaa taaacaatgg ttgctaccat atgcagcatt ttgttattta
      901 agagataaac acagtgattc gaatttcaga aactggaaaa acttttctgt gtttgattgg
      961 gacaaaatag atgatttctt taaaccagaa tttgccgatt ttgacgaggc aatgattcat
     1021 atctttttgc aatatcatgc tcacaaacaa ttacttgaag caagtaatta cgctaaatca
     1081 aaaggcattg tgctgaaagg tgatattcct ataggtatta gtccagatag cgtagaagcg
     1141 tggatggaac cacatttatt taacttagat agccaagctg gtgcaccccc cgaccctttt
     1201 tcagccactg gtcaaaactg gggtttccca acctataact gggaaagaat ggaaaaagac
     1261 ggataccaat ggtggaaaac tcgttttcaa aaaatggcag cttattttca agcatatcgt
     1321 atcgaccacg tgttaggttt tttccgcatt tggcgaatgt cagctgacga tgtgcaaggt
     1381 ttattaggtc attttgaccc agcattgcct ttaacagctg atgaaatcaa aggttttgga
     1441 acttggttcg actacgacag aatggtaaaa ccttacattc gcgaacacgt attgaatggt
     1501 ttatttggcg attataccaa cgaagtaaaa gagcgttttt tagacataga acaatatggt
     1561 gtgtatcgtt tcaaaaaagc atataataca caaaagaaaa ttgaagcatt ttttatcaaa
     1621 aatcaggata aaatggagga gcataaaata gctattttgc aaaatggctt atttgctttg
     1681 ttttgcgaag tattgtttat cgaagatagc aagaagcagg gaacatttca tcctcgcatt
     1741 gccttccatt caacgtattc gtatcgcgac ttagacaatg gggcgaaaaa tgccttgaat
     1801 aatttgtaca accattttta ttaccaaaaa cacaacgatt tttggaaaga acaagctatg
     1861 aaaaaacttc cagctttgct tcaggcgacc aatatgttgg tttgtggcga agatttagga
     1921 atgattccag aatgtgtgcc tagcgtaatg catgatttat acattatgag cttggaaata
     1981 gagcgtatgc caaaagatcc caaagaagaa tttattcaca tgccgaatgt accctattta
     2041 tcggtttgta ccacatctac acacgacatg aaccctattc ggggctggtg ggaagaagac
     2101 agaggtacta cgcaacgtta ttttaataac atactcaacg aatggggaga agctccgcaa
     2161 ttttgcgaac catgggtatg cgaaaaaatt gtagctcgtc acttgcattc acctgccatg
     2221 ttggttgtat tgccatttca agattggtta tctattaacg gtaaataccg ccgagaaaat
     2281 attttagagg aacgtatcaa tgtcccctct gatccaaaac atttttggtg ttaccgtatg
     2341 cattttacaa ttgaagaatt gcttgaaaat gacgaagtca acgacaaaat acttgcttta
     2401 acacaacaag ctggacgcaa ctcaacaatg taattttctc ccaaatagta aaaaagcgtt
     2461 ttgacatcca tcaaaacgct ttttttatcg cattatatcc aactatctag ctctttttca
     2521 attcaatacg caagcctaac tcatctaact gagcgttatc aattaccgaa ggtgcatcta
     2581 tcatcacatc tcttcccgag ttatttttgg ggaaagcaat gcaatcgcgg atgctatcca
     2641 atccagcaaa caacgacacc aatctgtcca acccaaaagc caaaccacca tgaggaggtg
     2701 ctccatactt gaaagcactc atcaaaaatc caaattgtgc ctctgcttgt tcgggtgtaa
     2761 atcctaataa ttcaaacatt ttcttttgca acgcactttc atgaatacgg atagaacctc
     2821 cgcccaactc tacgccattt atcaccatat cataggctgt agcacgtact ttgcctgggt
     2881 ttgtatccaa caatgcgata tcatcttcgt taggagaagt aaatggatgg tgcatagcat
     2941 aaaaacgttg tgtctcttcg tcccactgaa acaaaggaaa atcaattacc cacaatggtg
     3001 caaacacatt tttatctctc aaacccaaac gagtacccat ttctaaacgc aattcgcaca
     3061 aggctttttg tgttttttct gtggcacctg ccaaaattag tatcaaatcg cctttttcag
     3121 ccttacatgc tgttgcaatt ttagccaaat catcgttggt ataaaactta tctactgacg
     3181 acttaaacgt accatctgct tcgcagcgta tatacaccaa gcctttagct ccaatttgtg
     3241 gtcgttttac aaaatcagtc agttcatcta attgtttgcg ggtatatgtt gcacaattgc
     3301 tagcacaaat ggctcctaca taatcagcct cgtcaaatac aacaaaattt ttgcccgata
     3361 caagttcttt tatttctaca aattgcatat caaaacgtat gtctggtttg tcagaaccat
     3421 aatatttcat agcatcagcc catgtcatac gaggaaaagc atctgtcatg tcaatatttt
     3481 tcacaaaacg gaataaatgt ttcatcattc cttcaaacaa attcaataca tcttcttgtt
     3541 ttacaaacga catttcgcag tcaatttgtg taaactctgg ctgacgatcg gcacgtaaat
     3601 cctcgtctct aaaacatttt gctatctgaa aatacttatc aaaacccgac accatcaaca
     3661 attgtttaaa caactgcgga gactgtggca aggcataaaa ctgcccttgg ttcatacgcg
     3721 atggaaccac aaaatccctt gctccttcgg gtgtcgaagc gattaatatc ggtgtttcaa
     3781 cctcaagaaa tcccatttgg tcgagatagc gtcttgtctc aaacgcaacc ttatgacgca
     3841 atatcatgtt ttcacgcact gtattacgac gcaaatccaa gtagcgatac ttcatacgca
     3901 aatcatctcc accgtctgtg ttatcttcta tggtaaaagg aggtgtttcc gaagtattca
     3961 gcactcgcat agtcgataca agcaattcaa tttcacccgt agggatatgc ttatttttat
     4021 tgctacgttc agccacgtca gcttgcacct gtatcacaaa ctcacgtccc aattcatttg
     4081 cctgagcaca caaatcagca tttgtttctt ggttaaaaac taactgcgta ataccaaatc
     4141 tatcgcgtaa atcaacaaaa atcattccac ccattctacg cacccgttgt acccagccag
     4201 caagtgtaac ctgttgtcct atgtgtgata gtcgtaattc tccacaagta tgtgttctgt
     4261 acataataaa actctcttta tataattatg atgttgtgaa aaagaaaaca caaaagtaca
     4321 taaaattgcg atgttataaa aattttataa aattatttca aaatcttttg ctaaaataaa
     4381 aaagatagtt atctttgcat cccgttaagc gagacataat acctcttatt aacgagattt
     4441 tcggggtgta gcgcagcccg gctagcgcac ctgctttggg agcagggggt cccaggttcg
     4501 aatcctggta ccccgacaaa ttaaaaaaag cacttacatt ttattatgca ggtgcttttt
     4561 ggtttttagg aataaacata actttgtaag gctagcacat cccgattgtg tatcgggagg
     4621 gtcccaggtt cgaatcctgg taccccgaca attaaaaaaa gcacttacat tttattatgc
     4681 aggtgctttt tggttattat aacttcaagt ttatttgaac agacaatgtt ttatatatac
     4741 atactttatt cccaattaaa taataaatac tatattggtt caacccaaaa tactgaagaa
     4801 cgactaatta gacata
//
LOCUS       DAOSRP010000121         4693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_16573_length_4693_cov_3.677016, whole genome shotgun sequence.
ACCESSION   DAOSRP010000121 DAOSRP010000000
VERSION     DAOSRP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16573_length_4693_cov_3.677016"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..676
                     /locus_tag="PLJ82_07420"
     CDS             74..676
                     /locus_tag="PLJ82_07420"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOR41367.1"
                     /translation="MTTNKELMQQSLQVLQGRWGTPIAVAVLYCVIVMGVSMIPYVGT
                     VLSIVVGAPLVLGFYEFFLKLSRGEEVSTSILWTGFSFFDKSVLTYLLMFVYVLLWSL
                     LLIVPGIIASISYGMAFFILAEDKNLTPAEVLRRSKQMMYGYKWKYFCLGLRFIGWIL
                     LCIVTFGIGFLFLMPYMQTAYAKFYDDIKSASIPQESNEL"
     gene            complement(755..1360)
                     /locus_tag="PLJ82_07425"
     CDS             complement(755..1360)
                     /locus_tag="PLJ82_07425"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR41368.1"
                     /translation="MIINIHPQNPQEKEIKRVVDILENGGIIIYPTDTLYAFGCSIFD
                     QKAVNKICQLKQIDPKKVPLSFVCSNISHISEYATLHNNTFSLLKKNLPGAFTFLLKG
                     NHNLPRLFKEKKTVGVRIPDNKIVRSIVEILNHPIVSTSLFIDDNEPEYATDPDLIHE
                     KYGHIVDCVIDGGYGTLDVSTIVDCTEDSFEIIRQGKGILK"
     gene            complement(1402..>4693)
                     /locus_tag="PLJ82_07430"
     CDS             complement(1402..>4693)
                     /locus_tag="PLJ82_07430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOR41369.1"
                     /translation="HSTPQEGQAVTVTSTLNNSISTTSGNGAQYVYLAYSVNGGTYTS
                     TPMTVTATNTVYTGTIPSNVHVADNTISYYTYITTVDGLTPSTTGFDFLCLSQSSVNT
                     YNLNGGTSNPITIKAKRPSSWSNDMYLYFWGGEIASPSWDEASKMNSLGEGWYSFTLP
                     SDITASNILFRNGITSGLNNQSDDINEVSQSTCYNLSQSGSSKATASQVTCPVIVVGA
                     PTISAHKATVCENNFTDFASLYVSDYGILGSETGYAGGITFLWERSTDNVTWTTFTDG
                     RKATINNIRPTRTGYYRCKMTRFSTSTNSNEDIYSNVIQVTAGSNCTSRTVCSKLPVI
                     VVQTDVESFPTGYFLASNAAFQNAKKKISVDVKIIWDDTKDDTDNTMTEADFTNPNRL
                     YYDKKARMNYRGSSSLNYSKKNYAFVVGDDSCTLDGKWIKDTKKMFNINEVNKERDFI
                     LYAAYSDPTMMRNKLALDLYSDMTGSWNSKSRYVELYVDGEYKGVYIFMEKVKEDKGR
                     VQVQEDGYIFKFDKTDVWDRTTSTEGDRSTFVSNITGRQGITTYGLTVDHGFEIAYPE
                     WKEKDFTPEQWTTKWTYLRNRINDFENALKDGNYTAVNQYIDYESWADYFIMQEFAKN
                     IDGYRVSVWFTMETATSPIKMTPLWDNELAFNIKNITGKGNTSTTGLMVENSETRTDD
                     FAIPFWWTGEGKTNVGKGLLDDNCFKAKVKERWLKHTTTGGALSQTNIRAKIDDMQTS
                     LLASSCSSTPVSREFAANARWNTSWAADTTNINTWINPTTGRRKGLDDIISTWPTILS
                     SATINTVNNVNLGDNVTFTATVTGTGNYTYAWYFCATNSMDKTTWTLLNANGTSTYEI
                     TNIQSQHDGYYHCIVTSTTCSCISETLTSHLSLDTKTLTVISANTTQGTVTGGGSYTT
                     GSNATITALPKPGYRFLRWSDNNTNASRDITVSANATYIANFTLQTYTITLQGDNGTF
                     TGAGTYMYNNVATITAIPDTGYDFVKWSDNDTNASRSITVTEDKLFIAYFVEKIHTEI
                     VKQDTTDYIVYSNNQTIYIEAPENSHILIQDMMGRLMAKNATSLYVRNPGVYFIHINN
                     VIKKLIVN"
BASE COUNT         1346 a          806 c          831 g         1710 t
ORIGIN      
        1 ccaatttatt ctcaggtgct tacttttgtg cgttcggctt acagaaatta ttaatttatt
       61 caatttttat attatgacaa caaataaaga attaatgcaa cagtcattgc aagtattgca
      121 aggtagatgg ggaacgccta ttgccgttgc tgtattatat tgtgtaatag ttatgggagt
      181 atcaatgatt ccttatgtgg gaactgttct ttctatagta gtaggggctc cgttagtact
      241 cggattttat gaattctttt taaaattatc tcgaggagaa gaggtttcaa caagcatttt
      301 atggacaggt ttttcttttt ttgataaatc ggtgttaacc tatttattaa tgtttgtata
      361 tgtccttttg tggagtttgt tattaattgt acctggtatt atagcttcaa tttcgtatgg
      421 gatggctttt tttattttgg cagaagataa aaatttaaca ccagcagagg tgttgagaag
      481 gagtaaacaa atgatgtacg gctataaatg gaaatatttt tgtttaggtc ttcgctttat
      541 tggatggatt ttgctgtgta ttgtaacttt cggtattgga tttctatttt taatgcctta
      601 tatgcaaaca gcttatgcaa agttttatga tgacattaaa tcagcatcta ttccacaaga
      661 atcaaacgaa ttgtagcgtt cttgatgagt tagatagata aataaacaaa agaggattgt
      721 aatgcagtat gaacaatcct ctttttgctt tcctttattt tagtatgcct ttcccttgac
      781 gaataatctc aaagctatcc tcggtgcaat ctacgatggt tgaaacgtct agtgttccgt
      841 atccaccgtc aataacacaa tctactatgt gtccgtattt ttcgtgaatt aaatctgggt
      901 ctgtagcata ttcgggctca ttatcgtcaa taaatagtga tgttgatact attggatgat
      961 ttaaaatttc tacgatagaa cgtacaattt tattatcggg tatacgcacc cctacagttt
     1021 tcttttcttt aaataaacgt ggtaagttgt ggtttccctt tagcaaaaat gtaaatgccc
     1081 caggtaagtt ttttttcaga agactaaatg tattgttatg taaggtagcg tattcactaa
     1141 tatgactaat attactacat acaaatgata gtggaacttt tttagggtca atttgtttta
     1201 attgacaaat tttatttact gctttttgat caaaaatact acatccaaag gcatataagg
     1261 tatctgtagg ataaatgatg attccgccat tttctaaaat gtccacaacc cgttttattt
     1321 ctttttcttg tggattttgt ggatgtatat tgattatcat aaaatcgaat tttagtcaat
     1381 taatggaata ttgcaaaata tttaattcac aataagtttt ttaataacat tattgatatg
     1441 tataaaatat actcctggat tacgtacata taaagatgtt gcgttttttg ccatcagacg
     1501 tcccatcata tcttgtataa gaatatgact attttcaggt gcttcaatat agatcgtttg
     1561 gttattacta tacacaatat agtctgtagt gtcttgtttt acaatctctg tatgtatttt
     1621 ctctacaaaa taagcaataa ataatttatc ttctgttaca gtaatagaac gagacgcatt
     1681 ggtatcatta tcactccact tcacaaaatc gtaccctgta tcgggaatag cagtaatagt
     1741 tgccacatta ttatacatat atgtacctgc tccagtaaat gttccattat caccctgtag
     1801 agttatggta tatgtttgca gtgtaaaatt ggcaatatag gttgcatttg cggaaacagt
     1861 aatatctctt gacgcatttg tattgttatc cgaccagcgt aaaaaacgat atcctggttt
     1921 gggtaaagct gtaatagttg cattcgaccc agtagtataa cttccgcctc cagttaccgt
     1981 tccttgtgtt gtatttgctg aaattacggt caatgttttt gtgtctaatg aaagatgtga
     2041 tgtaagtgtc tcagaaatac acgagcaagt agtagatgtt acaatgcaat gataataacc
     2101 atcatgttgt gattgaatat ttgtaatttc ataggttgac gtaccatttg catttaatag
     2161 cgtccatgta gttttatcca ttgagttggt tgcacaaaaa taccaagcat atgtgtaatt
     2221 gcctgttccc gttactgttg cagtaaatgt aacattatca ccaagattta cattattgac
     2281 agtattaatt gtagcagatg ataaaattgt aggccaagta gatataatat catctaatcc
     2341 ttttcttctt cctgttgttg gatttatcca cgtatttata tttgttgtgt cagcagccca
     2401 agatgtgttc cagcgagcat tcgctgcaaa ttcgcgacta acaggtgtag atgaacaact
     2461 cgatgctagt aaagaggttt gcatatcatc aattttagca cggatatttg tttgagacaa
     2521 cgcacctcct gtggtggtgt gttttaacca acgttctttt acttttgctt taaagcagtt
     2581 gtcgtcaagc agtccctttc caacgtttgt ttttccttca cctgtccacc aaaaaggtat
     2641 agcaaaatcg tctgtccgag tttcagagtt ttcaaccatc aaacctgtag tggatgtatt
     2701 tcctttacct gtaatatttt ttatattaaa agctaattca ttgtcccaca gcggagtcat
     2761 tttgatagga gatgtggctg tttccatcgt aaaccataca gaaacgcgat aaccatcaat
     2821 gttttttgca aattcttgca taataaagta atcagcccac gattcataat caatgtattg
     2881 gttgacggca gtataattgc catcttttaa ggcgttttca aaatcattta tgcggttacg
     2941 cagataagtc cattttgtgg tccattgttc aggtgtaaaa tctttttctt tccattctgg
     3001 gtaggcaatt tcaaagccat gatcaactgt tagtccgtaa gtcgttatac cttgccttcc
     3061 tgtaatattc gacacaaaag tgcttctatc tccttctgtt gaagttgttc tatcccaaac
     3121 gtctgtctta tcaaacttaa atatatatcc atcttcttgt acctgtacac gtcctttgtc
     3181 ttcttttact ttttccatga atatatatac gcccttgtat tctccatcta cgtataattc
     3241 tacatagcga gatttactat tccaagaacc cgtcatgtcg ctatataaat ccaaggctag
     3301 tttgtttcgc atcatagttg ggtcagaata ggctgcatac aatataaaat cgcgttcctt
     3361 gttgacttca ttgatgttaa acattttttt tgtgtctttt atccattttc catctaatgt
     3421 acaactatca tctcctacaa caaatgcgta gttctttttt gaatagttta aactagaaga
     3481 acctcggtaa ttcattctag cttttttatc ataatacaaa cgatttggat tggtgaaatc
     3541 ggcttctgtc atagtattat ccgtatcatc ttttgtatcg tcccaaatta ttttaacatc
     3601 aacacttatt ttctttttag cattctgaaa agcggcattt gatgctaaaa agtagccagt
     3661 tggaaaactc tctacatctg tttgaacaac gattacaggc aatttactac aaacagtacg
     3721 tgatgtacag tttgatcctg ctgtaacttg tatgacattg ctataaatat cctcattgct
     3781 atttgtggag gtagaaaaac gtgtcatttt acaacggtaa tagcctgttc tagttggtct
     3841 aatgttgttt atcgtagctt ttcttccatc ggtaaacgtc gtccaagtaa cgttgtctgt
     3901 tgaacgttcc cataaaaacg taatcccgcc agcatatcct gtttctgaac ctagtatgcc
     3961 ataatcactt acatataaag atgcaaaatc cgtaaaatta ttttcacata ctgtagcttt
     4021 atgtgctgaa atggtaggtg ctcccacaac aataacagga catgttactt gcgaagcagt
     4081 tgcttttgaa gatccacttt gactcagatt ataacatgtt gattgtgata cttcattgat
     4141 atcatctgat tggttattta accctgaggt tatcccattt ctaaataaaa tattactagc
     4201 tgtaatatca gagggcaaag taaatgaata ccacccctca ccgaggctat tcattttgct
     4261 cgcttcatcc caacttggag aagctatttc acctccccag aaatataaat acatgtcatt
     4321 gctccaagag cttggacgtt tggctttgat agttattggg ttagatgttc ctccattaag
     4381 gttgtatgta tttacagaag attggctaag gcaaagaaaa tcaaagcctg tagtagatgg
     4441 ggttaatcca tccacagtgg taatataggt gtaatacgaa atggtgttat ctgctacgtg
     4501 aacatttgag gggattgttc ctgtatatac cgtgtttgtt gcagttactg tcatcggtgt
     4561 agatgtgtat gttccaccat taacactgta tgctagatat acatattgtg ctccattacc
     4621 cgaagtagtg gatatggaat tattgagtgt gctggtaact gttactgctt gtccttcttg
     4681 tggagtagag tgt
//
LOCUS       DAOSRP010000122         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_17518_length_4495_cov_4.802928, whole genome shotgun sequence.
ACCESSION   DAOSRP010000122 DAOSRP010000000
VERSION     DAOSRP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17518_length_4495_cov_4.802928"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PLJ82_07435"
     CDS             <1..932
                     /locus_tag="PLJ82_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOR41370.1"
                     /translation="EVISQEELGGARIHAEITGNAHFYAQSEFDCFDQIKKLISLLPA
                     SNTKRAAKVEPKVPSLKKQIGQIIPQDPKKPYDVRDVIRAVVDDSDFFEVQELFAPNI
                     VIGFARLNGDTVGFVANQPLYLAGVLDCDSSDKAARFIRFCDSFNIPLITLEDMPGYL
                     PGVDQEHAGVIRHGAKVLYAYSEATVPKVTVILRKAYGGGYIAMNSRHLGADFVFALP
                     SAEIAVMGPEGAANIIFAKEIKEAADPDAMRAAKVQEYIDKFANPFVAAAKGYIDAVI
                     EPNETRNLLIHTLSVSKDKDDYRPEKKHGIPPF"
     gene            996..1322
                     /locus_tag="PLJ82_07440"
     CDS             996..1322
                     /locus_tag="PLJ82_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOR41371.1"
                     /translation="MTEATKDSEFVDFAVTARKYKTLLTKKFINRKEWETPNPNNIKS
                     YIPGTIIELNVKPGQKVKQGELLLRLEAMKMQNRIDMPFDGIIKSVNVNVGERIPKNF
                     LMIEIE"
     gene            1452..2396
                     /locus_tag="PLJ82_07445"
     CDS             1452..2396
                     /locus_tag="PLJ82_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR41372.1"
                     /translation="MLSVLIPIYNQNCSKLIYALQQQIELLHVPVEIVAIDDASTVYK
                     EENKNALNHTRHRYIELQNNIGRAAIRNTLASMAKYDYLLFMDCDTYPASDYYLHNYI
                     QCLQSNSYQNGVICGGYKYSLQPPVDTQQLFRWKYGRNREQNLAVERNKFPYKSFSSF
                     NFCIPSKVFASIQFDETIKGYGHEDTWFGIALKENKVPVIHIDNELFQVSIDKTDIYL
                     EKSRNAVKNLYTLYTTSDKMSMFEQIKLIRAFEKIKKMRLCAMGAFLYDVSKENLEKN
                     FHSHHPLLVLFDFYRLGLLCKESLLARNNSLLPNKDEV"
     gene            2386..3615
                     /locus_tag="PLJ82_07450"
     CDS             2386..3615
                     /locus_tag="PLJ82_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOR41373.1"
                     /translation="MKYKICILFFYVSFTLFSQVVKTGIDVLQQRNFDVLSGKRVGLI
                     TNPTGVNKNLKSTVDILHEATNVKLIALYAPEHGVRGDIPAGEKVSNLIDTKTGVKVY
                     SLYGNTRKPTQEMLQGIDALVYDIQDIGCRSYTFISTLGLAMQAAAEKKIPVYVLDRP
                     NPLGGLKIEGNTTEDSFISFVSQFKIPYIYGLTVGELAYMLNEENMLGAKCELHIVPM
                     EGWKRSMRFNETELPWVLTSPHIPYMQSAVYYPASGILGELGIMSIGVGYTVPFQTFA
                     HEIFDAEKLSSSLNALRLHGVVFRPIHYKPYYGYGKDKNLKGVQVFFTNYTTARLSEV
                     QFYVLQVVKQLYPEFEVFTNENSSRWNMFDKVCGSDFIRIELQKNNYSFDKIKHYWRK
                     DEEDFKKKSTKYWIYKE"
     gene            3619..3921
                     /locus_tag="PLJ82_07455"
     CDS             3619..3921
                     /locus_tag="PLJ82_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HOR41374.1"
                     /translation="MDFLMHNNLIGVLIGISTFCIIGLFHPIVIKCEYYFGTRCWWLF
                     LLIGVVGICMSIIVKHILFSSLLAVFAFSSFWSILEIFEQKKRVDKGWFPKNPKKK"
     gene            complement(3954..4367)
                     /locus_tag="PLJ82_07460"
     CDS             complement(3954..4367)
                     /locus_tag="PLJ82_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOR41375.1"
                     /translation="MAKQEEINQGISHNTIAAGTVIKGEIRSEGDVRIDGSVEGTISC
                     KGKIIIGTKGEFKGTLTAVNIDVMGLIQGEVISENLLSIKSTGKIEGEVTTKTLIIEQ
                     EAILHGNCTMISNNENKPKLDVTISPIINKESKTN"
BASE COUNT         1542 a          635 c          828 g         1490 t
ORIGIN      
        1 gtgaagttat ttcacaagaa gaacttggtg gagcacgtat tcatgccgaa attactggaa
       61 atgctcattt ttatgctcaa agtgaatttg attgctttga tcaaataaaa aaattaatca
      121 gtttattacc agcaagcaat acaaaacgtg cggcaaaagt tgagcctaaa gttccatcat
      181 taaaaaaaca aataggacaa attataccac aagaccctaa aaaaccatac gatgtaagag
      241 atgtaatacg tgcggtagta gatgattcag atttttttga agtacaagaa ttatttgctc
      301 cgaatattgt tattggattt gcacgcttaa atggtgatac agtaggtttt gtagcaaatc
      361 aaccattgta tcttgcagga gtactagact gtgatagttc ggataaagcg gctcgtttta
      421 ttcgtttttg cgattcgttt aatataccat tgattacatt agaagatatg ccaggatatt
      481 tacctggagt agatcaagag catgctggtg ttattcgtca tggtgcaaag gttttatatg
      541 cttattcaga agctactgtg ccaaaagtaa ccgttattct acgtaaggct tatggtggtg
      601 gttatattgc tatgaattct cgtcacttag gcgctgactt tgtttttgca ttgccatcag
      661 cagaaattgc tgtaatgggt ccagaaggtg ctgcgaatat tatttttgca aaagaaatta
      721 aagaagctgc tgatccagat gcaatgcgtg ctgcgaaagt gcaagaatat attgataaat
      781 ttgcaaatcc attcgttgca gctgctaaag gatatataga tgctgtaatt gagcctaatg
      841 aaacacgtaa cttattaata catacacttt cggtatccaa agataaagat gattatcgtc
      901 ctgaaaagaa acatggaata cctccatttt aatgtgtact catattatat ttcatagctt
      961 aagtagtatt atttagtaac aaaaaatagt tagttatgac agaagcaact aaggacagtg
     1021 aatttgtaga ttttgcggta actgcacgta aatataaaac tttgctgaca aaaaaattta
     1081 taaacagaaa agaatgggaa actcctaatc ccaataatat aaaatcatat attcctggaa
     1141 caattattga gttgaatgta aaacctggtc aaaaggtaaa acaaggcgaa ttattgttac
     1201 gtttggaggc aatgaaaatg caaaacagaa tagacatgcc atttgatggt atcataaaat
     1261 ctgtaaacgt aaatgtagga gagaggatac caaaaaactt tttaatgatt gaaatagaat
     1321 agaaaaagtt gagtcagtta aaaaagtgaa gtctccaaat aaaggtagct tcactttttt
     1381 tttattcatg tatacaaata gtaattttct tcttatcttt atgaaccatt tttagtaata
     1441 ctcacttttg tatgctttct gtattaatac caatttataa ccaaaattgt agcaaattaa
     1501 tttacgcttt gcaacaacaa atagaactgc tacatgttcc tgttgagata gttgcaattg
     1561 atgatgcatc aacagtgtat aaagaagaaa ataaaaatgc tctaaaccat acaaggcaca
     1621 ggtatattga attacaaaat aatataggtc gtgcagctat tcgcaataca ttagctagta
     1681 tggcaaagta tgactatctt ctatttatgg actgcgatac atatccagca agcgattatt
     1741 atttacataa ttatattcaa tgtcttcaat caaattccta tcaaaacggc gtaatttgtg
     1801 gcggatataa atattcgctt caaccgccag tggatacaca gcagttattt cgttggaaat
     1861 atggaagaaa tagagaacaa aatctggctg ttgaaagaaa taagtttcct tataaatcgt
     1921 ttagttcgtt taatttctgt atacctagca aggtatttgc gagtatacaa ttcgatgaaa
     1981 caattaaagg atatggtcat gaagatacat ggtttggtat tgcactaaaa gagaataagg
     2041 tacctgttat acatattgat aacgaattat ttcaagtatc tattgataag actgatatct
     2101 atctagaaaa atctagaaat gctgttaaaa atctatatac attatacact acatcagata
     2161 aaatgagtat gtttgaacaa attaaattga taagagcatt tgaaaagata aaaaagatgc
     2221 gattgtgtgc tatgggagct tttttatatg atgtgagtaa agaaaatctt gaaaaaaatt
     2281 ttcattctca tcatccatta ttagttttat ttgattttta tcgtttagga ttattgtgta
     2341 aagagagttt attagcaaga aacaacagtt tattacccaa taaagatgaa gtataaaatc
     2401 tgtatattgt ttttttatgt atcgtttaca ctattttcgc aagtagtaaa aacaggtatt
     2461 gatgtattgc agcagagaaa ctttgatgtt ttaagtggta aaagagttgg gttgataacg
     2521 aatcccactg gagtaaataa aaatcttaaa tccactgtgg atatattaca tgaggcaact
     2581 aatgttaagc taattgcatt gtatgcccca gaacatggcg taaggggaga tattcctgca
     2641 ggcgaaaagg taagtaattt gatagataca aaaacgggtg taaaagtata ttctttgtat
     2701 ggcaatacac gcaaaccaac gcaagaaatg ctacagggaa tagatgcatt agtatatgat
     2761 attcaggata ttggatgccg ttcatatact tttattagca cactagggct tgctatgcaa
     2821 gctgcagctg aaaagaagat acctgtatat gtgttagacc gacctaatcc gttaggagga
     2881 ttaaaaattg aaggaaatac tacggaagac tcgtttattt cttttgtgag tcaatttaaa
     2941 attccataca tctatggatt aacagtagga gagttggcgt atatgttgaa tgaagaaaat
     3001 atgttagggg ctaaatgtga attacatatt gtaccaatgg agggctggaa acgatctatg
     3061 cgttttaatg aaacagaatt accttgggta ttgacttctc cgcatattcc gtacatgcag
     3121 tctgctgtat attatcctgc ttcaggcatt ttaggcgagt taggaataat gtctattgga
     3181 gtaggatata ctgtgccatt tcagaccttt gctcatgaaa tatttgatgc agaaaaacta
     3241 tcatcaagtc taaatgctct tcgtttacat ggagttgtat ttcgcccaat tcattataag
     3301 ccgtattatg gatatggtaa agataaaaat ctaaaaggag tacaagtgtt ttttacaaat
     3361 tacacaacgg cacgtctgtc agaggttcag ttttatgtat tgcaggttgt aaaacagtta
     3421 tatccagaat ttgaggtttt tacgaatgaa aatagcagta gatggaatat gtttgataag
     3481 gtatgtggta gtgattttat tcgtatcgaa ttacaaaaaa ataactattc ttttgataaa
     3541 atcaaacact attggagaaa agatgaggag gattttaaga agaaatcaac caaatattgg
     3601 atttataaag agtaaattat ggacttttta atgcacaata atttaattgg tgtactgata
     3661 ggtataagta cattttgtat tattggatta tttcatccca tcgtaattaa atgtgagtat
     3721 tattttggaa cacgttgttg gtggttattt ttgttaatag gagttgtagg tatttgtatg
     3781 tctattatag taaaacatat tttattttcc tccttattag ctgtttttgc tttttcttct
     3841 ttttggagta tattggaaat tttcgaacaa aaaaaacgcg tggacaaagg gtggtttcca
     3901 aagaatccta aaaaaaaata gcattgttaa aaataaaata aagaaagcaa aacttagtta
     3961 gttttgcttt ctttgtttat aataggggaa atggtaacat ccaattttgg tttgttctca
     4021 ttattactta tcattgtgca gtttccatgt aagatagctt cttgctcaat aataagagtt
     4081 ttggtggtaa cttctccttc tatttttcca gtagatttaa tcgataataa attttctgat
     4141 attacttctc cttgaattag gcccatcaca tcaatattta ctgcggtaag tgttccttta
     4201 aactcccctt ttgtgccaat aataattttc cctttgcaac taatagttcc ttcaacagaa
     4261 ccatcaattc ttacatcgcc ttcagaacgg atttctcctt taattacggt acctgcagca
     4321 atggtattgt gcgaaatacc ttggttgatt tcttcttgtt ttgccatgat tttaggtttt
     4381 aaaatgcatt aaatttaaaa aaaaatccca cctaaatggg attttgagcc ggcagaggga
     4441 ttcgaaccca cgaccccgag attacaaatc acgtgctctg gccaactgag ctatg
//
LOCUS       DAOSRP010000123         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_17776_length_4440_cov_6.425998, whole genome shotgun sequence.
ACCESSION   DAOSRP010000123 DAOSRP010000000
VERSION     DAOSRP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17776_length_4440_cov_6.425998"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..994
                     /locus_tag="PLJ82_07465"
     CDS             77..994
                     /locus_tag="PLJ82_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOR41376.1"
                     /translation="MALVYGADKLVEAAASFAKKLQIPSIVIGLTIVSFGTSAPEFVV
                     NLFASLENNSDIVLGNILGSNIANILLILGTSAIIYPLTVKKQTTWIEIPLSVMAAIA
                     VFFLASDQLIDHATQSAMTHIDGMMLLLFFLVFIAYNISLTIKSKNDEDIETKDYSIL
                     VSTLLIVLGLTLLGAGGKAIVYGATNLAETFGISQRIIGIIIVGIGTSLPELATSVVA
                     ARKQKTDIAIGNIVGSNIFNTFFVLGTSATISSVQVSSAAMYDIYVNIACSVLLFGLL
                     FIGKSHTISRKEGFIMLGLFITYIASITL"
     gene            complement(1001..3250)
                     /locus_tag="PLJ82_07470"
     CDS             complement(1001..3250)
                     /locus_tag="PLJ82_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR41377.1"
                     /translation="MGEIHLVSDLAIILIAAGIVTLLFKRLKQPMVLGYIVAGFLVGP
                     YFDVLPTIIEKESVHQWSEIGVIFLLFALGLEFSFKKLMHVGSSALITAGTEVVTMFS
                     LGTLVGYLLHWTMIESFMLGGMLSMSSTTIIIKAFDDLGIRKHKFTSIVFGTLVVEDI
                     VAIVMMVLVSTAALSKEFAGIDMLYSIVKLAFFLVLWFLVGIYVFPSFFQKARKWMND
                     ENLLIISIGFCFGMVAMATYTGFSAALGAFIMGSIFAETVEGEHIEKLSKGIKDLFGA
                     IFFVSVGMMVDPSVLVEYWFPVLLLTLSTIFGKAFFSFLGVLLSGQDLRTAIQSGMSL
                     AQIGEFAFIIATLGYTLGIMSPHIYPIIVAVSVISTFTTPYMIRLANPLYEWIYPRLS
                     SNTKETLAKYVTYSNRVTTSSDWKQLLRAFAVQVIGYTVICVGIILFMFHFVHPFLLE
                     KLNVWMPNVLINLFQALLTIAFLFPFLKGLMVNRNRKQDIFLKLWNENKVTRGGLMSL
                     LLLRVFIAVVLVTFVLYSCFTHSYLFVILVALAIVLFILFSRVSFLHYAKIEKRFIAN
                     LHTKEELDKQQKPLRTSFYSHFAHQNIHLAVVETSVDSPYIGQKISETNIKEAFGVSI
                     IKIIRGKNFIYLPTGEERIYPYDQLLVLGNDEQIEQLNTVMEQKEDIDIPKANIDVTL
                     TSFIVQEGSSLIGKSIIQSGIRNVGCMIIGIERHNLTIMNPDSYEIIQLADIIWIVGD
                     KQNIQKTMC"
     gene            complement(3334..4089)
                     /locus_tag="PLJ82_07475"
     CDS             complement(3334..4089)
                     /locus_tag="PLJ82_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOR41378.1"
                     /translation="MAEKKTEIEISIEQKLRALYDLQTIYSDIDRIKTLRGELPLEVQ
                     DLEDEIIGLQTRITKYDADVEELTNRIVNRKVDIQNATALITKYQEQINNVRNNREFD
                     HLTKEVEYQTLEIEHCEKNIREFTVAIDVKKEEKAKALAQLEDMNANLKEKKEELEEI
                     VSETRQEEEKLRDDAKQLEKLIEDRLLQAFKRIRKNARNGLAVVYIQRDACGGCFNKI
                     PPQRQLDIRLRKKIIVCEHCGRILVDSELANAE"
     gene            complement(4137..>4440)
                     /locus_tag="PLJ82_07480"
     CDS             complement(4137..>4440)
                     /locus_tag="PLJ82_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR41379.1"
                     /translation="CKMLRTSTLTNKMIKKVAVCGGSGRSLIQQAINHKADAYVTADI
                     SYHDFFLPENRLLLVDIGHFESEHFIKDIFFDLISKKMPTFAIHFSENEHNPVLYF"
BASE COUNT         1506 a          841 c          631 g         1462 t
ORIGIN      
        1 tgcagttctt cgattgaact tttgtgctaa aaatccgcca ctacgccaag ccgccaatcc
       61 gttacttggt ggttttgtgg cattggtcta tggtgcagat aagcttgttg aagcagcagc
      121 tagttttgcc aaaaaattac aaataccaag tatcgttatt ggtcttacaa ttgtttcttt
      181 tggcacctct gctcccgaat ttgtcgtcaa tctgtttgcc tcgcttgaaa ataacagcga
      241 tatcgtttta ggaaatatct tggggagtaa tattgctaat attctactca tactcggaac
      301 atcagctatt atataccctt taacagtaaa aaaacaaact acttggatag aaatcccact
      361 aagtgttatg gctgctattg ctgttttctt tttagcttca gatcaattga ttgatcacgc
      421 tactcaatca gcaatgactc atattgatgg aatgatgtta ttattgttct tcttagtttt
      481 tatagcgtat aatatctctc ttaccattaa aagtaaaaat gacgaagata ttgaaacaaa
      541 agattactct attttagttt caactctact cattgtactt ggattaacat tgttaggggc
      601 aggaggaaaa gctatagtgt atggagctac taatttggca gaaacctttg gtatcagtca
      661 acgcatcatt ggtattatta ttgttggaat aggaacatca ttacccgaat tagcaacaag
      721 tgtggtagct gctcgtaaac agaaaacaga tatagcaatt ggcaatattg tcggttctaa
      781 tattttcaat actttctttg tattaggaac ttcagctacc attagttcgg tacaagtatc
      841 ttcggctgca atgtacgata tctatgttaa tatagcatgt agtgtcttgt tatttgggct
      901 tttatttatt ggaaagtcac atactatatc tcgtaaagaa gggtttataa tgcttggctt
      961 gtttattaca tatatcgcat ccattacatt atagaaaacc tcaacacatc gtcttttgta
     1021 tgttttgttt atcccctaca atccaaataa tatcagctag ttgaataatc tcatacgaat
     1081 ctggattcat tatggtaagg ttgtgtctct ctattccaat aatcatacac cccacgtttc
     1141 tgataccgga ttgaataata cttttaccga ttaaagacga gccctcttgt actataaatg
     1201 acgttaacgt aacatcaata tttgcttttg ggatatctat gtcttctttt tgttccatca
     1261 ctgtattcaa ctgttctatt tgttcatcat tgcccaatac caataactga tcatatgggt
     1321 aaatacgctc ctctcctgtc ggcaaataaa taaaattttt tcctcttata attttaataa
     1381 tggatacacc aaaagcctcc ttaatattag tttcactaat cttttgtcca atatatggcg
     1441 aatcaacaga tgtttcaaca accgctaaat gtatattttg atgtgcaaaa tgtgaataaa
     1501 aacttgttcg taaaggtttt tgctgcttgt ctaattcttc tttcgtgtgt aaatttgcaa
     1561 taaatctctt ttctattttc gcataatgta aaaacgaaac tctagaaaac aatataaaca
     1621 agacaatagc caatgctact aaaataacga acaaataact atgtgtaaaa caagaatata
     1681 atacaaaagt tactaataca acagctataa aaacacgcaa caataataat gacatcaatc
     1741 ctcctcgtgt aactttattt tcattccata gttttaaaaa tatatcttgt tttctatttc
     1801 tgtttaccat taatcctttt aaaaaaggaa ataaaaatgc aatagtcaac aaagcctgaa
     1861 aaagattaat caatacatta ggcatccaga catttaattt ttctagtaga aaaggatgaa
     1921 caaaatgaaa cataaataaa atgataccaa cacaaatcac ggtatagcca attacttgca
     1981 cagcaaatgc tctcaataat tgtttccaat cgctactagt cgtcacccta tttgaataag
     2041 ttacatattt ggctaatgtt tctttggtat tgcttgataa acgcggatat atccactcat
     2101 ataaaggatt agctaatcgt atcatgtaag gagttgtaaa ggtagaaata accgatactg
     2161 ctactataat aggatatata tgcgggctca ttatgcctaa agtataaccc aatgttgcta
     2221 taataaacgc aaactctccg atttgagcta aactcatacc tgactgaatg gctgtacgta
     2281 aatcttgacc agaaagtaac acccccaaaa aggagaaaaa ggctttgcca aagatcgtcg
     2341 ataacgtaag caataacaca ggaaaccaat attcaactaa tacagatggg tcaaccatca
     2401 taccaacaga tacaaaaaat atagcaccaa ataaatcttt aatgccttta gacaattttt
     2461 caatatgctc tccttccact gtttcggcaa aaattgaccc cattataaat gctcccaatg
     2521 cggctgagaa acctgtataa gtagccattg ctaccatccc aaaacaaaac ccaatggaaa
     2581 taatcaacaa attttcatcg ttcatccact ttcgagcttt ttgaaaaaaa gagggaaaaa
     2641 cataaatccc aaccaaaaac cacaatacca agaaaaaggc taatttaaca atgctgtata
     2701 acatgtcaat acctgcaaat tctttactta aagcagctgt tgatactaaa accatcataa
     2761 caatggcaac tatatcttca acgactaagg taccaaaaac aatagaggta aatttatgct
     2821 tgcgaattcc taaatcgtca aacgccttga taatgatggt agttgatgac attgaaagca
     2881 taccaccaag cataaaactt tcaatcattg tccaatgaag taaataccct accaatgtgc
     2941 ccaacgaaaa catcgttaca acttcagttc ctgctgtaat aagagctgaa ctgccaacat
     3001 gcattagttt tttaaaacta aactcaagcc ccaatgcaaa caaaagaaaa atcacaccaa
     3061 tttcagacca ctgatgcaca ctctcctttt ctataattgt gggtaaaaca tcaaaataag
     3121 gtcccactaa aaatccagcc acaatatatc ccaaaaccat aggttgctta agtcgcttaa
     3181 aaagcaacgt aacaatacca gcagcaatta agataatggc taaatcagaa actaaatgta
     3241 tttcccccat acaaaaataa tgacattaag taggataaaa ataaaaaaaa tagcctgaca
     3301 catatatcag gctattaact ataaaaatag aatttattca gcgttagcta gttcggaatc
     3361 aactaaaata cgtccgcaat gttcgcatac aataattttt ttacgtaggc gaatatccaa
     3421 ctgtctttga ggtggaattt tattaaaaca accgccacat gcatcacgtt gaatatatac
     3481 cacagctaaa ccattacgtg catttttacg aatacgttta aatgcttgca ataaacgatc
     3541 ttctatcaat ttttccaact gcttagcatc atctcgaagc ttttcttctt cttgtctagt
     3601 ttctgataca atttcttcta attcttcttt tttctctttc aaattagcat tcatatcttc
     3661 tagctgagca agagcttttg ctttttcttc tttttttaca tcaatggcaa ctgtgaattc
     3721 acgtatattt ttttcacaat gttcaatctc aagtgtttga tattcaacct cttttgtcaa
     3781 atggtcaaac tctcgattat ttcttacatt attaatttgt tcttggtact ttgttatcaa
     3841 agcagttgca ttctgaatat ctacttttct atttacaatt ctgtttgtca actcttctac
     3901 atcagcatcg tatttggtaa tacgagtttg caaaccaatg atttcatctt ctaaatcttg
     3961 tacttctaaa ggaagttcgc ctcttaacgt ttttattctg tctatatcag aataaatggt
     4021 ttgtaaatca tacaaagctc tgagtttttg ttctattgaa atttcaattt cagtcttttt
     4081 ttcagccata ctacaggtta ttttgtgggt attatctcaa ataaaatgct tatttatcaa
     4141 aaatagagaa caggattatg ctcattctct gaaaaatgga ttgcaaatgt aggcattttt
     4201 tttgaaatta aatcaaaaaa aatgtctttt ataaaatgtt cagactcaaa atgtccaata
     4261 tcgaccaaaa gtaaacgatt ttcgggtaaa aaaaagtcat gatacgaaat atctgctgtt
     4321 acataagcat ctgccttgtg atttattgct tgttgtatca acgaacgccc actacctcca
     4381 cacacagcaa ctttttttat catcttattt gttaaggttg acgtacgtag catcttgcat
//
LOCUS       DAOSRP010000124         4381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_18054_length_4381_cov_4.475728, whole genome shotgun sequence.
ACCESSION   DAOSRP010000124 DAOSRP010000000
VERSION     DAOSRP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18054_length_4381_cov_4.475728"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLJ82_07485"
     CDS             <1..314
                     /locus_tag="PLJ82_07485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOR41380.1"
                     /translation="HNGISRQFKNANFDRGKLDDLWLIKRGKKITDTHSAVFPEELVK
                     TIIENFSNEGDLIYDPFMGTGTTAIVAKKLNRNYLGSELTEKYFKTIQERLCVANNEL
                     F"
     gene            335..556
                     /locus_tag="PLJ82_07490"
     CDS             335..556
                     /locus_tag="PLJ82_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41381.1"
                     /translation="MNKQRVDSAVIKSVSLRLKQLREAKGISQETLYIDTDIHIARIE
                     AGKSNITISTLSALCKYLDISLADFFEGI"
     gene            complement(645..2141)
                     /locus_tag="PLJ82_07495"
     CDS             complement(645..2141)
                     /locus_tag="PLJ82_07495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOR41382.1"
                     /translation="MNTKNVKWLWVAVATFVVSVAVATLISFFIVKNHGLGNQAFLSS
                     KQHAPSTFVAMSEDSPIASHGFVKAAEATVNTVVHVRTKSPAKQVAFSRDPFFEFFFG
                     YPQMERQEPRMQEGAGSGVIISSDGYIVTNSHVVASSTNIEVTLNDKRTFEATLIGSD
                     PNTDIALIKIDAKDLPVSVFGDSDALSVGEWVLAVGNPFNLTSTVTAGIVSAKARSIN
                     ILSGDMKIESFIQTDAAINPGNSGGALVNLQGELVGINTAIATTTGAYAGYGFAVPSS
                     IVQKVVVDLKQYGTVQRAVLGVQIADITSELAKEKGLKTLDGVYIHSVVENGAADNAG
                     LKSEDIIQQINGIRVNTVAQLQEQLSRFRPGDQVTIQVLRKNKELSMRATLKNKLGNT
                     DVVGSQVNAEEYLSIRVKEIDNQTKSQLRLSYGLQITEIKKGKLFDQGIKNNYIIVSV
                     NNQAIRTKDDLQQALDTALNAADRNKVLFITGVYPNGVRAYYAINLDE"
     gene            complement(2328..3065)
                     /locus_tag="PLJ82_07500"
     CDS             complement(2328..3065)
                     /locus_tag="PLJ82_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOR41383.1"
                     /translation="MKAMIFAAGLGTRLKPLTDSMPKALVPINGKPLLEHVLLKLKQE
                     GFTDIIINVHHFADQIVQFLAQNQHFGLNIAISDETQQLLETGGGIKKASWFFGNEPF
                     LIHNVDILSNLPLAELVRFHSSKPNAAATLVVSKRTTTRYLLFDNDMQMKGWTNIQTN
                     EIKSSYPQTIIQACSEFAFSGIHVFSPALFPYFEAYPAKFSVIDFYIDVCGKAAVYGY
                     VPTNLQLLDVGKIDSIQQAELFVQTMY"
     gene            complement(3078..>4381)
                     /locus_tag="PLJ82_07505"
     CDS             complement(3078..>4381)
                     /locus_tag="PLJ82_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOR41384.1"
                     /translation="AGSITAFTLTNLTAWSGNQFVSKGLPVPRVVGVSDDEMCYLVED
                     LGNVSLFDSIKKGRETGEFSAQDIELLKKTIAYLPSLQIEGANGFDFSICFPQAAFNY
                     RTVMWDLNYFKYCFLKPAGIECNEEWLENDFEHLASFLLQDKSNTFMYRDFQSRNVMI
                     RNGNPYFIDFQGGRKGPIQYDVASFVYQAKANFSAEVRETLIASYLEALSKYMPVDEV
                     AFRKQLAFFVLFRNLQVLGAYGFRGLVEKKKHFVESIPFAIQNLNEILKNNYFDTHFP
                     YLVSVLQQLIVCYTHQDKPTSPSNLVVKVSSFSYKKGIPEDTTGNGGGYVFDCRAIHN
                     PGKYEQYKQLTGLDKPVIDFLEQDGEILAFLEHIYALADSSVSRYVERGFSSIMFSFG
                     CTGGQHRSVYAAQHVAEYIATRYSVKVELVHREQGIKQQFN"
BASE COUNT         1354 a          881 c          787 g         1359 t
ORIGIN      
        1 gtcataatgg aattagtcgt caatttaaaa atgcaaactt tgatagaggg aaattggatg
       61 acttatggct tattaaacga gggaaaaaga ttactgatac gcattctgcc gttttcccag
      121 aagaattagt taaaactata attgagaact tttctaatga gggtgattta atttatgatc
      181 cttttatggg aacaggaaca actgcaattg ttgcaaaaaa attaaatcgt aattatttgg
      241 ggagtgaatt aaccgaaaaa tattttaaga ctattcaaga gagattatgt gtagccaata
      301 atgaactatt ttaattacta cacttttgta ggttatgaat aaacaacggg ttgatagtgc
      361 tgtaattaaa tcggtatcac tgcgtttaaa gcaattgaga gaggcgaaag gaatttctca
      421 agaaacttta tacattgata cggatataca tatcgctcgt atagaagctg gcaaaagcaa
      481 cattaccatt agtaccctat ccgctttgtg taagtattta gatatttcat tagctgattt
      541 ttttgaagga atataatggg cttctactat ttactccgaa acaataaaag cgaataaccc
      601 acataggtta ctcgctttat atattcaatc attcagactg gttattactc gtccaaatta
      661 atggcatagt acgctcttac gccattggga tatacgccag taataaataa taccttattg
      721 cgatcggcag catttagggc tgtatctagg gcttgttgca aatcgtcttt ggtgcgaata
      781 gcttggttgt ttaccgacac aatgatgtag ttgtttttta taccttggtc gaataattta
      841 ccttttttga tttcggtaat ttgcaaacca tacgacaacc ttaattgaga tttagtttga
      901 ttatcaattt cctttacccg tatcgaaaga tattcttcgg cattcacttg cgaacccact
      961 acgtctgtgt tgccaagttt atttttaaga gtagccctca tacttaattc tttattcttt
     1021 ctaagtacct gtatagtaac ctgatcgcct ggtctaaaac gacttagttg ttcttgcaat
     1081 tgagccaccg tatttaccct tatgccgtta atttgctgaa taatgtcttc ggatttaagt
     1141 cctgcattat cggctgctcc attctctact accgaatgga tatatacacc atctagggtt
     1201 tttaagcctt tttctttggc taattcgctg gtaatatcag caatttgaac acccaacaca
     1261 gcacgttgca cagtgccata ttgttttaaa tctacgacta ctttttgcac aatactggat
     1321 ggcacagcaa acccataacc agcatatgct ccagtcgttg tagcaatagc ggtattaata
     1381 cctaccaatt ccccttgtaa attcaccaac gcacctccgc tattccctgg attgatggct
     1441 gcgtctgttt ggataaatga ttcaattttc atatcgcccg acaagatatt gatgctgcgt
     1501 gccttggcac taacaatacc tgctgttacg gtagaagtga ggttaaatgg attacccaca
     1561 gccaataccc actcacctac actaagtgca tcggagtcgc caaatacgga caccggcaaa
     1621 tctttggcat caatttttat cagggcaata tcggtattgg gatcagagcc aattaaggtc
     1681 gcctcaaaag tacgtttatc attcaacgta acttctatat tggtagaact tgctaccaca
     1741 tggctattgg ttacaatata cccatcagaa gagataataa cccccgaacc tgctccttct
     1801 tgcatacgtg gctcttggcg ttccatttgg ggatatccaa aaaagaattc aaaaaatggg
     1861 tcgcgcgaaa aagcaacttg cttggcaggc gattttgttc gtacgtgcac tactgtattt
     1921 acggtagcct cggctgcttt tacaaaacca tgcgaggcaa tgggactatc ctccgacatg
     1981 gcgacaaagg tggacggagc atgctgtttg cttgataaaa aagcttgatt gcccaatcca
     2041 tgatttttga caataaaaaa actgatgagg gttgctactg ctacactgac tacaaaagta
     2101 gctaccgcaa cccatagcca ttttacattt tttgtgttca tacttgttat tattaaattg
     2161 ttttttattg ggtttgttta tgctcaaaaa taaacaaaaa atacgccaat aagaccttgt
     2221 atcactaggt tttatcaatt tttaatactt ggattttggg cattaacggg tattaaccca
     2281 gcaggcagaa atcgtaaaaa ttttttaata aaaaagctga caaaaaatca gtacatagtt
     2341 tgtacaaaca attcagcttg ctgaatactg tcaatttttc ctacatctaa caactgcaaa
     2401 ttggtaggca cgtagccata cacagcagcc ttgccacata catcaatgta aaaatcaatt
     2461 acagaaaatt tagcggggta agcctcaaaa tagggaaata atgcgggcga aaacacatga
     2521 atgccactaa atgcaaactc actacaggct tgtataatag tttgtggata agaagatttt
     2581 atttcgttgg tttgtatatt cgtccaccct ttcatctgca tatcattatc gaacaacaag
     2641 taacgcgttg tagtacgttt acttaccacc aaagtggctg ccgcattagg tttactggaa
     2701 tgaaatcgta caagttcagc taagggaaga ttcgacaaga tatctacgtt gtgtatcaaa
     2761 aaaggttcgt tgccaaaaaa ccaagatgct tttttgatgc ctcctcctgt ttcgagtaat
     2821 tgttgtgttt cgtctgagat ggcaatgttg agtccgaaat gttggttttg agccaaaaac
     2881 tgaacaatct gatcagcaaa atgatgcacg ttgatgatga tatccgtaaa accctcttgt
     2941 ttgagtttaa gcaaaacatg ctcaagtagc ggttttccat taatgggtac aagagctttg
     3001 ggcatactat cagtaagcgg tttcaaccgt gtacccaatc ctgctgcaaa aatcatcgct
     3061 ttcatacgta tggtattcta attaaactgt tgtttaatgc cttgttcccg atgtactaat
     3121 tccactttta cagaatagcg tgtagctata tattcggcta catgttgtgc tgcatataca
     3181 gagcgatgtt gtcctccggt gcaaccaaaa gaaaacataa ttgaagaaaa acctctttct
     3241 acatacctgc taaccgaact atcagctaat gcataaatat gctcaagaaa ggctaaaatt
     3301 tcgccatctt gttccaaaaa atcaatcacg ggtttgtcta atcctgttag ttgcttatat
     3361 tgctcgtact taccaggatt atgaatagcc ctacaatcaa agacgtaacc acctccgttg
     3421 cccgttgtat cttcgggtat acctttttta tacgaaaaac tacttacctt taccactaag
     3481 ttcgatgggc tggttggctt atcttggtgt gtatagcaaa cgattaattg ctgtaatacg
     3541 ctaacgagat acggaaaatg agtatcaaaa taattatttt tgaggatttc atttaaattc
     3601 tgaatagcaa aagggatgct ttctacaaaa tgctttttct tctccactaa tcccctaaaa
     3661 ccgtaggctc caagtacttg caaattccta aataacacaa agaatgcaag ttgtttgcga
     3721 aaagccacct catctacagg catatacttc gacaatgcct ccaaatagct tgcaatcaag
     3781 gtttcacgaa cttctgccga aaaattggct ttggcttgat acacaaaaga agctacatcg
     3841 tattgaattg gaccttttcg tcctccctga aaatcaataa aataaggatt tccgttgcgt
     3901 atcatcacat tgcgactctg aaaatcgcga tacataaacg tgttactttt atcttgaagc
     3961 aagaatgatg ccagatgttc aaaatcattt tctaaccatt cttcgttaca ttcgatacca
     4021 gcaggtttta aaaaacaata tttaaagtaa ttcaaatccc acatcaccgt acggtaatta
     4081 aaggctgctt gcggaaaaca gatggaaaaa tcgaaaccat ttgccccttc aatttgcaac
     4141 gacggtaaat aagcaattgt tttttttagt agctcaatgt cttgtgcact aaactctcct
     4201 gtttctctgc ctttcttaat cgaatcgaat aacgatacat tacccaaatc ttcaacaaga
     4261 tagcacatct catcatctga cacacccact acacgtggca cgggtaagcc tttggaaaca
     4321 aactgattcc ctgaccatgc agtcaggttc gtcaaggtga aggcagttat agaacctgca
     4381 c
//
LOCUS       DAOSRP010000125         4180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_19169_length_4180_cov_4.437576, whole genome shotgun sequence.
ACCESSION   DAOSRP010000125 DAOSRP010000000
VERSION     DAOSRP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19169_length_4180_cov_4.437576"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="PLJ82_07510"
     CDS             <1..1836
                     /locus_tag="PLJ82_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOR41385.1"
                     /translation="MLNLACDFPSYVVFYNGPACGASAPDHLHFQMGNKGFLPIEKDY
                     DTLPKNPVNSFSYLQYQSIETDFYHAIVIESDTKQEVEKRFSFLYKQLQKTSAEPMMN
                     VVCWHTGTNFVVIVLPRTKHRPTQFFATDDTKIVLSPASVDLGGVLITPIEKDFTTLQ
                     LADIVDISSQVCASVEDISNKLQHPCVSVGILSSPSIAFSFAGSPYFCTTINEQVQGE
                     CIVSCVNGKILFKNQWYNELCFEPNDANVDTFLVYDVVIGVDFHWQRKENQTFKGTLR
                     FVVEQDNIVLINTLPVEEYLLSVISSEMSATSSISLLKAHAVISRSWLFAQIQQVCSS
                     QAETLSIEGNTMIKWYDHHNHLLFDVCADDHCQRYQGVAKVTTNQVQKAIEETYGEVL
                     VYQNNLCDARFSKCCGGVTEEYATCWENNQVAYLQSIVDEKQEEKKLDLRTESAITSW
                     IKSSPTVFCNTSDAHILSQILPHFDQETTDFFRWKKVYSQHELSTLVHKRSGIDFGVI
                     HDIRPIKRGKSGRLEIIEIVGTTRTMRIGKELEIRKWLSDTHLYSSAFIVERDDERNF
                     IFYGAGWGHGVGLCQIGAAVMSEQGYSYKEILQHYYTGVDILSVY"
     gene            2020..3285
                     /locus_tag="PLJ82_07515"
     CDS             2020..3285
                     /locus_tag="PLJ82_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR41386.1"
                     /translation="MKNKPISPWAWIPSLYFGQGIPYVVAATTFSLIMYKNMGISNAD
                     AALYTSWLYLPWVLKPFWSPFVDLIKTKRWWILLMQFVIGIALAGVAFAIPLPYFLQS
                     TLAILWLIAFSSATHDIAADGFYMLALDTHEQSLFVGIRSTFYRVAMIVGQGALVMLA
                     GYLETTMPSIAIAWSITFYGIAVFFFALVLYHWFVLPKPSTDIVRNDKRVVTDFFATF
                     GAFFTKKGIWLSIVFILLYRLGEAQLVKIASPFLLDPRSVGGLGLTTSEVGFVYGTIG
                     VIALTLGGILGGIVAAKDGLKQWLWIMVIAMNLPNLVYVYFAYMQPDNFYIIASGVAI
                     EQFGYGFGFTAFMLYLIYIAKGEFQTAHYAIATGFMALGMMLPGMFAGILQEYMGYFP
                     FFVWVCICTIPGFLLIPFLKIDPSFGKKE"
     gene            3292..4107
                     /locus_tag="PLJ82_07520"
     CDS             3292..4107
                     /locus_tag="PLJ82_07520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOR41387.1"
                     /translation="MNTTKYKMLVLDLDDTLLRDDYSISERTINALLTTQQIGVKVVL
                     ASGRPTLAMTQYAKMLQQHDFDSYMISFNGAVVSSTRTNEVIFETSLTQSEIHSLYDF
                     SVEHGVHIITYSDKGIVSETHSEYIDVELKLTGIPHYKVPSFKDEVSYSAIKCILLEH
                     PDYLKQVELVLKSQRTDLSVSRSKPFFLEVMPNGIDKAASLDMLAKKLGYAPEQIIAV
                     GNAGNDLSMIQYAGVGVWVDNVTPELRHFGDYIVASNNNDGVAEVVERFILIK"
BASE COUNT         1252 a          670 c          873 g         1385 t
ORIGIN      
        1 atgctcaatt tggcttgtga ttttccgtcg tatgtagttt tttataacgg acccgcatgt
       61 ggagcttctg ctcctgatca tttgcatttt caaatgggta ataaagggtt tttacccata
      121 gaaaaagact atgatacctt gcctaaaaac ccagttaact ctttttctta tcttcaatat
      181 caatcaatag aaacagattt ttatcatgct attgttatag aatcggatac caagcaggag
      241 gttgaaaaac gtttctcttt tttatataaa caactacaaa aaacaagtgc agagccaatg
      301 atgaatgtag tatgttggca tacaggcaca aattttgtgg taatcgtatt gccacgcacc
      361 aaacaccgac caacacagtt ttttgctacg gatgacacaa aaatagtgct aagtccagct
      421 tcggtagatt taggtggggt attgattaca cccatagaaa aagattttac tacattgcaa
      481 ttagctgata ttgtggatat ttcgtcgcag gtatgtgcat ctgtagagga tataagcaac
      541 aaactacaac acccttgtgt aagtgttggt attctttctt ctccttctat agcgttttct
      601 tttgcgggct ccccttattt ctgtactact atcaacgagc aagtgcaagg cgaatgcata
      661 gtttcgtgtg tcaatggtaa aattttgttt aagaatcaat ggtataatga attgtgtttt
      721 gagccgaacg atgctaatgt agatacattt ttggtttatg atgtagtaat tggtgtggat
      781 tttcattggc aacgaaaaga aaaccaaacg tttaagggta cattacgttt tgttgttgaa
      841 caagacaaca ttgtattaat taatacctta cctgttgaag agtatttgct gagtgttatt
      901 tcatctgaaa tgagtgctac atcttctatt tccttattaa aagctcatgc tgttatatct
      961 cgtagttggc tttttgcaca aatacaacaa gtctgttcaa gtcaggctga aactttgagt
     1021 attgagggga atacgatgat aaaatggtat gaccatcaca atcatttact atttgatgtg
     1081 tgtgctgacg atcattgcca acggtatcag ggcgtagcga aggtaactac aaatcaggtg
     1141 caaaaagcta ttgaagaaac atatggagag gtgttagtat atcaaaataa cttgtgtgat
     1201 gcacgctttt caaaatgttg tggaggcgta accgaagagt atgctacgtg ttgggaaaac
     1261 aatcaagttg cttatttaca atcaatagta gatgaaaaac aggaagagaa aaagttggac
     1321 ttacgtactg agtcagcaat aacatcttgg attaaatcat caccaactgt attttgtaat
     1381 acttctgatg ctcatatttt gtctcaaatt ctgcctcatt tcgatcagga aactacagac
     1441 ttttttaggt ggaagaaagt gtatagtcaa cacgaattat cgacattagt acacaaacga
     1501 tcgggtattg attttggagt tatacacgat atacgtccca ttaaacgtgg aaaatcgggt
     1561 cgattagaaa ttattgagat tgtaggaaca accagaacta tgcgaattgg aaaagaatta
     1621 gagattcgta agtggttatc agatacgcat ttatatagtt cagcattcat cgttgagcga
     1681 gatgatgaaa ggaattttat tttttatggg gcaggctggg ggcatggtgt aggtctttgc
     1741 cagataggag cagctgtaat gagtgaacaa ggatattcgt ataaagaaat tttgcagcat
     1801 tattacacag gagtggatat actttccgta tattagtctt tttgttacac tttgtatact
     1861 attatttcac gaagaacacg aaggaaacac gaagttacac tgagagaaaa acatattact
     1921 ccgtgtccct ctgtgaaacc tctgtgtacc tctgtgaaag aatcttacac gaagaagaaa
     1981 attaattaac gccaattact agtagataaa aaaatatata tgaaaaataa acctatttca
     2041 ccatgggcat ggattcctag cctgtatttt ggacaaggaa ttccttatgt agtagctgcc
     2101 actacttttt ctctcattat gtacaaaaat atgggtattt caaatgccga tgcagcctta
     2161 tatactagct ggttgtattt gccttgggta cttaaaccct tttggagccc gtttgtcgat
     2221 ttaatcaaaa caaaacgctg gtggatattg cttatgcagt ttgtgatagg aatagctttg
     2281 gctggcgttg ccttcgccat tcccttgccc tattttttgc aaagtacatt ggctatatta
     2341 tggttaatag cctttagttc agctacgcac gatatagcag ctgatggatt ttatatgtta
     2401 gccttagata cgcacgagca atcccttttt gtcggcattc gaagtacctt ttatcgtgtt
     2461 gcgatgattg tggggcaagg agccttggta atgcttgctg gttatttaga aacaaccatg
     2521 ccctctatcg ccattgcatg gagcattaca ttttatggaa tagcagtttt tttctttgca
     2581 ttggttcttt atcattggtt tgtattacca aaaccatcaa cggatatcgt tagaaatgat
     2641 aaacgtgtag ttacagattt tttcgctaca tttggtgctt ttttcactaa aaaaggcatt
     2701 tggttgtcca ttgtgtttat attgctctat cgcttgggag aagctcaatt ggttaaaatt
     2761 gcatctccat ttttacttga cccacgtagt gttgggggct taggattaac tacctctgaa
     2821 gtgggttttg tatatggtac cataggtgta attgcgctta cattaggggg tattttggga
     2881 ggtattgtgg cagctaaaga tggactaaaa cagtggttgt ggataatggt tatagccatg
     2941 aacttaccca acctagtgta tgtgtatttt gcttatatgc aacctgacaa tttttatatc
     3001 atagcttcag gtgtggctat agaacaattt ggctatggat ttggtttcac tgcctttatg
     3061 ctgtacctta tatatattgc caaaggagaa tttcaaacgg cacattatgc tatagctact
     3121 ggttttatgg ctttaggaat gatgttgccg ggcatgtttg caggaatttt gcaagagtat
     3181 atgggttatt tcccattttt tgtatgggtg tgtatttgta ccattcctgg gtttttatta
     3241 ataccatttt tgaaaattga tccatcattt gggaagaaag aataacaaga aatgaacaca
     3301 actaagtata aaatgttggt gctcgactta gatgatacct tacttcgaga cgattatagt
     3361 atttcagaga gaaccatcaa tgctctttta acgacgcaac agattggggt aaaggttgta
     3421 ttagcctctg gcagaccaac cttagctatg actcaatatg ctaagatgtt acaacaacac
     3481 gattttgact cctatatgat ttcgtttaat ggagcagtcg ttagttctac cagaacgaat
     3541 gaagtaattt ttgaaacaag tttaacccaa tcggaaatcc acagtttata cgattttagc
     3601 gttgagcatg gtgtacatat tattacgtat tcagataaag gcattgtgag cgaaactcac
     3661 tctgagtata ttgatgtaga actaaaactg acaggaatac ctcattacaa agtgcctagc
     3721 tttaaagatg aagttagcta ttcggctata aaatgtattt tgttagagca tccagattat
     3781 ttaaaacaag tggaattggt acttaaatcg caacgtacag atttgagtgt gtctcgttct
     3841 aagccgtttt ttttagaggt gatgcccaat ggaatagata aagctgctag tttggatatg
     3901 ttagccaaaa aacttggcta tgctccagag caaattattg cagtaggcaa tgcaggcaac
     3961 gatttatcga tgattcagta tgcaggggta ggcgtgtggg tggataatgt aacgcccgaa
     4021 ttaaggcatt ttggcgatta tattgttgct tctaataaca atgatggtgt agcagaggtg
     4081 gtagagcggt ttattctgat aaagtaaaga cgtatgcctt tatacgattg atacatcagt
     4141 aattttacat ttaaaaattc ttgaggtaat tattttagcc
//
LOCUS       DAOSRP010000126         4044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_20002_length_4044_cov_5.677864, whole genome shotgun sequence.
ACCESSION   DAOSRP010000126 DAOSRP010000000
VERSION     DAOSRP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20002_length_4044_cov_5.677864"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..734)
                     /locus_tag="PLJ82_07525"
     CDS             complement(93..734)
                     /locus_tag="PLJ82_07525"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOR41388.1"
                     /translation="MKFSITDEHEVKKFFIIFYVVGIIGMAIPYTLPLFKILTPYALV
                     LSFGYLAYFHPTKKNTTSNILFFFIFLAGLVVEIIGVKTGVIFGGYEYGNSLGMKVFD
                     TPLLIGINWLFLVYTTACMVDKTKWHAVLKIVVAASGMLVYDIVLEQVAPKMDMWSWQ
                     DNVVPIQNYIAWFVIAAFFHSLLKIFKVKLSNPLAAHIIACQFVFFVVLLFLL"
     gene            complement(747..1586)
                     /locus_tag="PLJ82_07530"
     CDS             complement(747..1586)
                     /locus_tag="PLJ82_07530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOR41389.1"
                     /translation="MMELYTNIAFKTSELVTKTYSTSFSIAISCLPYETKKAIYAIYG
                     FVRFADEIVDTFEKTQQKNLIRDFEADYQKALETGISMNPILHAFVLTIQKYNIPLNL
                     VDSFLHSMKLDLDKQIYTTDAELREYIYGSADVVGLMCLHIFVNGDKKEYDNLLLPAK
                     RLGSAFQKVNFLRDLKADYEYLGRAYFPDFSIETFNEAKKRSIINDMETDFIEARKGI
                     SELPGKTKLAVYIAYLYFYELLKKIKKTPVHGILEARIRVSDTKKIALFAKAYIDFLL
                     HKI"
     gene            complement(1591..2130)
                     /locus_tag="PLJ82_07535"
     CDS             complement(1591..2130)
                     /locus_tag="PLJ82_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOR41390.1"
                     /translation="MKRIKTFSIICSTMAFFSLTACSTIPKGVEAVTAFEQERYLGKW
                     YEIARFDFRFERNLNNTTAFYELNSDNSIKVVNRGYNYVTNEWKEATGKAKFVEKNDV
                     AMLKVSFFGPFYAGYNVIAIDKNYQYALVSGSSLKYLWILSRTPQLPQNIKEEYLQLA
                     HEKGFKVENLIWVEHNQQE"
     gene            complement(2274..2846)
                     /locus_tag="PLJ82_07540"
     CDS             complement(2274..2846)
                     /locus_tag="PLJ82_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41391.1"
                     /translation="METNEKKNVTEKVNEISTKSKLTFWGFIKKHPTFTTIIVALLSI
                     GAVILYKDASHAKWQKEMTEKASNQLSNTQEDLIKLVAKPLVWNIRAEMLRNNMEQVS
                     ILMTDMVRDNTIQYVHLIDTNGLVLLSTNKKLEGQIITNEFDSSLLKTTEPTTLVIEN
                     IVQVAAPVMGVDRQFGVLILGYQPTMLVLD"
     gene            complement(2945..3787)
                     /locus_tag="PLJ82_07545"
     CDS             complement(2945..3787)
                     /locus_tag="PLJ82_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOR41392.1"
                     /translation="MQTHYTKYVTKASGEKQEFSAKKLVQSLMNAGANEDTAKNILNE
                     IEKVMVDGMHTKKIYTIAFGLLKKERIQLALKYKLKKAIMELGPTGHPFENFIGQLFE
                     KRGYKTEVGVVVHGACIQHEMDVIATKPPFQYILECKYSQDQSKQVAIQVPLYVHSRV
                     EDIIDYRKKLPIYEGVEFITGVVTNTRFTTDSIQYGKCKGMYLLSWDYPANENLKQLV
                     DSLKLYPITILNSLSKKEKETLINAGYITCNQLLSNHEWDKELYLTPQKTLLIQKELD
                     LITS"
     gene            complement(3884..>4044)
                     /locus_tag="PLJ82_07550"
     CDS             complement(3884..>4044)
                     /locus_tag="PLJ82_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOR41393.1"
                     /translation="SAIRQGQVCANGIAAIGSGHDLAGKTLILAVFPELYAIISLAAA
                     YMIGSGVI"
BASE COUNT         1304 a          693 c          625 g         1422 t
ORIGIN      
        1 aaagtgcctt tttattttcc aaaaggagta gaaaataaaa aatgggtaaa taaaccaatg
       61 atgctttgct tttatcatct ttttatccac atttaaagta aaaataacaa tacaacaaaa
      121 aatacaaatt ggcaagctat aatatgagca gccagcggat tgctcagttt tactttgaaa
      181 attttaagca aactatgaaa aaaagcagca attacaaacc atgctatgta gttttgtatt
      241 ggcaccacgt tatcttgcca cgaccacata tccattttgg gagccacttg ttctaacacg
      301 atatcataca ccaacattcc agatgctgca actactattt tgagtactgc atgccatttc
      361 gttttatcga ccatgcaagc ggtagtatac actaagaata accaatttat accaatcaat
      421 aggggtgtgt caaaaacctt catacccaaa ctattaccgt attcgtatcc accaaaaatt
      481 acacctgttt taacgccaat aatttcaaca actaaaccag caagaaaaat aaagaagaat
      541 aaaatattgc tcgtagtgtt ttttttagtt ggatgaaaat aagccaaata cccaaaactt
      601 aatacaagtg catagggagt taaaatttta aataaaggaa gtgtataggg gatagccatg
      661 ccaattattc ctacaacata aaatataata aagaactttt ttacttcatg ttcgtctgtt
      721 atcgaaaatt tcatatatga tatgggttat attttgtgta aaaggaaatc aatgtatgct
      781 ttggcaaata gtgcgatttt tttagtgtcc gaaactcgga tacgtgcttc taaaatgcca
      841 tgtacgggtg ttttttttat ttttttcaac agttcgtaaa aatataaata ggcaatataa
      901 acagctaatt ttgtttttcc aggcaattcg cttattccct tgcgagcttc tataaaatcg
      961 gtttccatat cgttgataat actacgtttt ttagcctcat taaatgtttc aatgctaaag
     1021 tctggaaaat aagctcgacc taaatattca taatctgctt ttaagtcgcg taaaaagtta
     1081 accttctgaa aagcggagcc taatctttta gctggaagta atagattatc atattctttt
     1141 ttatctccat ttacaaatat gtgcaagcac atcaatccaa caacatctgc tgaaccataa
     1201 atatattctc tcaattctgc atcggtcgta tatatttgct tgtcaaggtc aagtttcatg
     1261 ctatgtaaaa acgaatctac taagtttaga gggatattgt atttttgaat agttaataca
     1321 aaagcatgta aaatagggtt catgctaatg cccgtttcca aagctttttg gtaatctgct
     1381 tcaaaatcgc gaatcaggtt tttttgttga gttttttcaa aagtatccac aatttcgtcg
     1441 gcaaaccgta caaacccata aatagcataa atggcttttt ttgtttcgta tggcaagcaa
     1501 ctaatagcaa tagaaaaaga ggtactatag gttttagtaa ccaattcact tgttttaaat
     1561 gcaatgtttg tgtatagttc catcatacaa ttattcctgt tgattatgtt ctacccaaat
     1621 taaattttct actttaaagc ctttttcgtg agcaagttgt aggtattctt ctttgatgtt
     1681 ttgtggtagc tgaggtgtgc gagataatat ccacaagtat ttaagactac taccagatac
     1741 caatgcatat tgataatttt tgtcgatagc aatcacatta taacctgcat aaaaaggacc
     1801 aaaaaaagaa accttgagca tagcaacatc atttttttct acaaactttg ctttccccgt
     1861 tgcttctttc cattcgttgg ttacataatt ataaccgcgg ttaacgacct taatgctatt
     1921 gtcggaattt aattcgtaaa aagcagttgt attgttgaga ttacgctcaa atcggaagtc
     1981 aaaacgtgct atctcatacc actttcctag gtatcgttct tgctcaaaag ctgttactgc
     2041 ttctactcct ttgggtatag tactacaagc tgttaagcta aaaaaagcca ttgtactaca
     2101 aataatgctg aaggtcttta ttcttttcat atccgataat tatttacaca aagataaggg
     2161 aaaaatgaat actatttcaa aagaaattct ttgaaatgcg acagctttat ggtgtactat
     2221 gtactaaaaa atgcgagaag caattctcac cttttttaga gtatatcttt ttgctagtct
     2281 aaaaccagca tggtaggttg ataaccgagt attaaaacac cgaattgcct atcaaccccc
     2341 attacaggag ctgctacttg tactatattt tctatcacca aagttgttgg ttctgtggtt
     2401 ttaagcaaag aactgtcaaa ttcgttggta ataatttgtc cctctagttt tttattggtt
     2461 gataaaagaa ctaatccgtt cgtgtcaatt aaatggacgt attgaatagt attatctctc
     2521 accatatctg tcattaatat tgaaacttgc tccatattat tgcgtagcat ttctgcccga
     2581 atattccaaa ctaaaggttt cgctactagt tttattaaat cttcttgtgt gttagataat
     2641 tgattactag ctttctcagt catttctttt tgccacttag catggcttgc atctttatac
     2701 aaaatcacag ctccaatgct tagtaatgca acaatgatag tagtaaacgt agggtgtttt
     2761 tttataaatc cccaaaaagt aagtttggat tttgtggata tttcatttac tttttcagta
     2821 acattttttt tctcgtttgt ttccataatt gataagtata tatacacaca aatataacaa
     2881 agaaaataca attatgcaat aatagaggaa taaaaaattt ttttctataa aaacaagttc
     2941 ctttttatga agttattaaa tctaattctt tttgaatgag tagtgttttt tgaggtgtta
     3001 gatagagctc tttgtcccat tcatgattag ataataattg attgcatgta atgtatccag
     3061 catttattaa tgtttctttc tctttttttg atagagaatt aagtatggtt atagggtata
     3121 actttaaaga atcaaccaat tgtttcaaat tctcgttggc tggataatcc cacgataata
     3181 aatacatccc tttacacttt ccatactgga tactatccgt agtaaagcgg gtatttgtaa
     3241 ccacacctgt aataaattct actccctcat aaatgggtaa tttttttcta taatcaataa
     3301 tatcttctac acgcgagtgt acatataagg gtacttgtat ggctacttgt tttgattggt
     3361 cttgcgagta tttacattct aagatatatt gaaagggagg tttcgtggct attacatcca
     3421 tttcgtgttg aatgcaggct ccatgtacaa caaccccaac ctcagtttta tatcctcttt
     3481 tttcaaataa ctgaccaata aaattttcaa atggatgtcc agtaggacca agctccatga
     3541 tggctttttt caacttatac tttaatgcta gttggatacg ttcttttttt aatagtccaa
     3601 atgcaatagt atatattttt ttagtatgca tgccatctac cattactttt tctatttcat
     3661 tcaaaatatt tttggctgta tcttcattgg cacctgcatt cataagcgac tgtactaatt
     3721 ttttggctga aaattcttgt ttttcgccag atgctttagt aacatacttt gtataatgtg
     3781 tttgcataga atagttacat gagggttgaa taacaaataa aggcagaaac agagttctac
     3841 ctttatgaaa aaataataga gcaatacctt tgcattaatg tttttatatc acgccagacc
     3901 caatcatata cgcagcagct aatgaaatga tggcatataa ttcggggaat acagccaaga
     3961 ttaatgtttt tcctgccaaa tcatgacctg aaccaatagc agcaataccg ttggcacata
     4021 cttgaccttg gcgtatagct gata
//
LOCUS       DAOSRP010000127         4012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_20210_length_4012_cov_4.065201, whole genome shotgun sequence.
ACCESSION   DAOSRP010000127 DAOSRP010000000
VERSION     DAOSRP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20210_length_4012_cov_4.065201"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1896
                     /locus_tag="PLJ82_07555"
     CDS             163..1896
                     /locus_tag="PLJ82_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOR41394.1"
                     /translation="MTKKTETVRLITFFKEIFYPKLFSSLRLYSKEKFLSDTMAGVIV
                     GIVAIPLAIAFGIASGVGPTEGLITAVIAGFIISFFGGSKVQIGGPTGAFIVIIYGII
                     QQHGLTGLLVATIMAGVMLVLMGVFKLGSIIKFVPYPVIVGFTGGIALTIFSTQINDF
                     FGLGISNLPADFIHKWISYFSHFTAIHWWSFIIALISLFIILVMPLFSKKIPGSLVAI
                     ILATILVYFLKLTSFAGVDGIKTIQDIYILPEGIPMPHLPSIALAENQTLWGLIQTLF
                     PAAFTIAMLGAIESLLSAMVADGVIRDKHNSNTELIAQGMANIVVPFFGGIPATGAIA
                     RTMTNINNGAKTPIAGIIHAIVLLLVLLFLGKFVGYIPMACLAAVLIIVAYHMSGWRS
                     FISLSKNPKSDFVVLMTTFLLTVIFDLTIAIEIGLLLAVVLFLKRTNEATIIRVFQHK
                     IDPAENSDILLQDDPLYIPKEVDVYEIDGPYFFGIATKFDEVMETVGEKPKVRIIRMR
                     KVSFIDSTGIHHLQNLCEQSHREKIVIILSGVNKHVRGALMKANFFSILPEERVCSTI
                     HEALELSEQYI"
     gene            2207..>4012
                     /locus_tag="PLJ82_07560"
     CDS             2207..>4012
                     /locus_tag="PLJ82_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOR41395.1"
                     /translation="MWKSHTSTKWSVLKPQASEIAESFTLVWLPPSAVAEGGGTSNVG
                     YHPYKWSNQSSSWGTRTQLEALITDLKAGGAKVLADIVVNHRAGNGWCDGFTTDNFGT
                     YGVFTLKASHITKDDEASGQSACSGKLSDHNDWNFDKPIEYWGGCQAARDLAHSLTEV
                     RDAVKAYLIWMKNEIGYDGFRWDLVKGFNPAYIKEYTQAAGAYLSVGEYYSPNYNDLK
                     GWVEATDKTALVFDFGFKNAIFQWGGGSDYSKLGWKDGSILRPAGLVHNPGMRQYAVT
                     FVDNHDTSSPHPDGAGWEYKGDKAKANAIMLAAPGIPCVFWRDWVAVKADIKKMIAAR
                     KKVGLNSNSDVRVTNTNGYYESHGVGTTGELICRVGSWSGAPSGFTLECSGTGWAYYT
                     KGGSTAPTITVSPQGGYVGVNGKVTITASTGSTIYYTTNGTNPTSSSTQYTGQITITT
                     NNTRIKAIAIKGAESSAIADYTFLTEQPAGVDLQFKAPVAWSTVKAYVWTGEGAGATP
                     VNGAWPGGSINKVGDYYTYTYTGTLPVNIVFNNGVDGGPQTIDLTANAATCWDGSAGG
                     AMITPVACGGSVETGGDYYVAGNPELCGVEWGVSAA"
BASE COUNT         1192 a          584 c          846 g         1390 t
ORIGIN      
        1 ccaatagcgt cgatggtact gcacttgtgt gggagagtag atagccgccg ttttttacca
       61 aaggaggaga tgtttttata cgtctcctcc tttgtcgttt tagaatttat taggtctatt
      121 ctttactcta taatttgtct gattttaacg ttttatacat ttatgactaa aaaaactgaa
      181 actgtaagat taatcacttt ttttaaagaa atcttttatc caaaattatt ttcttcttta
      241 cgtctttatt ctaaggaaaa gtttttgtct gacactatgg ctggtgtaat agtgggtatt
      301 gttgctatac cactggctat tgcatttggt attgcatcag gagtcggtcc tactgaggga
      361 cttattactg cagttattgc tgggtttatt atatcatttt ttggaggttc aaaagtgcag
      421 attggaggtc caacgggagc atttattgta attatttatg gtataattca acaacatggt
      481 cttacgggat tattggtagc taccattatg gctggtgtaa tgttggtatt gatgggggtt
      541 tttaaattag gttctattat taagtttgta ccttatcctg ttattgttgg ttttactggt
      601 ggtattgcac ttacaatttt ttctacccag attaatgatt tttttggttt aggaatatct
      661 aatttacctg cagattttat tcataaatgg atttcttatt tttcccattt tacagcaata
      721 cattggtggt cttttataat tgcattaatt agtttgttca ttattcttgt tatgccttta
      781 ttttcaaaaa agatacctgg ttctttggtt gcaatcatat tagctactat ccttgtttat
      841 ttcttaaaat taacatcatt tgcaggagta gacggaatta aaacaatcca agatatctat
      901 attttaccag aaggtattcc aatgcctcat ttgccatcaa ttgcattagc agagaatcaa
      961 acgttatggg gattgataca aacgttattc ccagcagctt ttacgatagc tatgttaggg
     1021 gctattgaat cgttactttc tgcaatggtt gcagatggag ttatacgtga taaacataat
     1081 tctaatacgg agttgattgc acaagggatg gcgaatattg tagttccttt ttttggaggt
     1141 atacctgcta caggagctat tgctagaacg atgactaaca tcaataatgg tgctaaaacc
     1201 cctattgcag gaattataca tgctattgtt ttattgttag ttcttttatt tttaggaaaa
     1261 tttgttggat atattccaat ggcatgttta gctgctgtct taattattgt ggcatatcat
     1321 atgagtggat ggagatcgtt tatatcttta tcaaaaaatc caaaatcaga ttttgtggtt
     1381 ttaatgacaa cttttttact aacggtaatt tttgatttaa ctatagcgat tgaaatagga
     1441 ttattattag cagtagtatt attccttaaa agaaccaacg aagcgactat aatacgagtt
     1501 tttcaacata aaattgatcc tgctgagaat tctgatattc ttcttcaaga cgacccatta
     1561 tatattccca aagaggttga tgtttatgaa attgatggac cttatttttt tggtatagct
     1621 accaaatttg atgaagtaat ggaaacagtg ggagaaaaac ccaaagttcg tataattcgt
     1681 atgcgtaaag tttcttttat tgattctacg ggcattcatc atttacaaaa tttatgtgaa
     1741 caatcgcata gggaaaaaat agtaatcatt ttgtctggtg tgaataaaca tgtacgagga
     1801 gcattaatga aagccaattt ttttagtata ttaccagaag aaagagtttg ttccacaatt
     1861 catgaagctt tagaattatc tgaacaatac atttgataga tgttttataa ggtagacaga
     1921 tatataaagt ctcacaacgt tatcatgtag aaaaagaaaa ttacatagaa gtgctaatat
     1981 tgtttattcc aagaatgtta aatctcatca aaatgtggag aagatatggg ataattcatt
     2041 tttttagcta aatttttagt ctaaaagtta attaaacctt aatattttat acaatgaaaa
     2101 aacaaaatca cttttactgt atgtcttttt gtctcttttt ggcatatttt ctcaggcata
     2161 cgctcaggtt cctttggctt ctcatgatgt tatgcttcag ggatttatgt ggaagtcaca
     2221 tacgtctact aaatggagtg ttctaaaacc tcaagcgtct gaaatagctg agtcgtttac
     2281 tttagtatgg ttaccaccgt cagcggttgc cgaaggtggc gggacgtcta atgtaggata
     2341 tcacccttac aaatggtcta atcagtctag ttcgtggggt acacgtactc agttagaagc
     2401 cttaattaca gatttaaaag caggtggagc taaagtatta gctgatattg tggtaaatca
     2461 tcgtgcaggt aatggttggt gtgatggttt tacaacagat aattttggta cctatggtgt
     2521 ttttacttta aaagcttcac atattacgaa agatgacgaa gcttcaggac aatcagcatg
     2581 ttctggtaag ttaagtgatc ataatgattg gaattttgat aaaccaatag aatattgggg
     2641 aggatgtcag gcggctcgcg atttagctca tagtcttacc gaagtgagag atgccgtaaa
     2701 agcatattta atatggatga aaaatgaaat tgggtatgat ggttttcgtt gggatctggt
     2761 aaagggtttc aatcctgctt atattaaaga atatacacaa gcagcaggag cttatttgtc
     2821 tgtaggtgag tattatagtc ctaattataa tgatttaaaa ggttgggtag aagcaactga
     2881 taaaacggcc ttagtgtttg actttggatt taagaatgct atttttcaat ggggtggagg
     2941 ctctgattac agtaaattag gttggaagga tgggagtatc ttacgtcctg cgggattggt
     3001 acataatcct ggtatgcgtc aatatgctgt tacatttgta gataaccatg atacgtcatc
     3061 tcctcatcca gatggtgctg gatgggaata caaaggagat aaagccaaag ccaatgctat
     3121 tatgttagca gcacctggga ttccttgtgt attttggaga gattgggtag ccgtaaaagc
     3181 tgatatcaaa aaaatgattg ccgctagaaa aaaagtcggt ttaaatagta actctgatgt
     3241 gcgagtgact aatactaatg gatattacga atcgcatgga gtaggtacta ctggcgaatt
     3301 gatttgtcgt gttggtagtt ggagtggtgc tccaagtgga tttactcttg agtgtagtgg
     3361 aacaggttgg gcttattata ccaaaggagg aagtactgct cctactatta cagtttctcc
     3421 tcaaggaggt tatgtgggag taaacggaaa agttactata actgcttcta ctggaagtac
     3481 gatttattat acgactaatg gaactaatcc tacgtcttct tctactcaat acactggtca
     3541 aattactatt acgaccaata atactcgaat taaagcaata gctattaaag gggctgagtc
     3601 ttctgctatt gctgattaca catttttaac agaacaacca gcaggggtag atttacaatt
     3661 caaagcccca gtagcttgga gtactgtaaa agcatatgta tggacaggag aaggtgcagg
     3721 tgcaacgcca gtaaacggtg catggccagg cggttctatt aacaaagtag gtgattatta
     3781 tacatataca tataccggaa ccttacctgt gaatattgta tttaataatg gtgtagatgg
     3841 tggaccacaa actatcgacc ttactgcaaa tgctgcaact tgttgggatg gaagtgctgg
     3901 tggtgctatg attacgcccg tagcttgtgg tggttcagtg gagactgggg gagattatta
     3961 cgttgctggt aatcccgaat tatgtggtgt tgaatggggt gtaagtgctg ct
//
LOCUS       DAOSRP010000128         3960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_20556_length_3960_cov_5.634571, whole genome shotgun sequence.
ACCESSION   DAOSRP010000128 DAOSRP010000000
VERSION     DAOSRP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20556_length_3960_cov_5.634571"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLJ82_07565"
     CDS             <1..255
                     /locus_tag="PLJ82_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41396.1"
                     /translation="SEGLLLLLVGLLSVFVVLLVVMGISRWLILLVNRYFPETTSRVD
                     NKLIQEPKEHVSSSDSMVIDEVIKKLSQGTFVVKKIEKIK"
     gene            261..2093
                     /locus_tag="PLJ82_07570"
     CDS             261..2093
                     /locus_tag="PLJ82_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOR41397.1"
                     /translation="MKKEVKFSLVYRDMWQSAGKYMPRVDQLVRVAPEIIRMGCFARV
                     ETNGGGFEQINLLFGENPNVAVRQWCKPFNDAGIQTHMLDRSLNALRMYPVSEDVRRL
                     FYKVKKAQGTDIARSFCGLNDTRNIIASIRYAKEAGMIAQAALSLTVSPVHTVDYYCA
                     MADELIANGADEICLKDMAGIGRPVSLGKIVAYIKKKYPTIIVQYHGQTGPGFTPASI
                     LEVCKAGCDIIDVGMEPLSWGTGHADVIMVREMLKDAGFSVPEINMSAYMKVRALTQE
                     FMDDFLGYFIPETNRHLQSLLIAPGLPGGMMGSLMNDLKDNLVSINKAYQKQGKTTIN
                     TDELLIQLFDEVAYVWPRVGYPPLVTPYSQYVKNLAMFNIIQMAKGKARWTMISDDTW
                     DMILGKAGRLPGELAPEIKELATQQGRTFYEGDPQILYPNVLENFRKEMIDNGWDMGK
                     DEEELLELAMHPEQYRTYKSGKAKHDFEIELAQAKKESQQRGDGSFVLPHVLEMTVNG
                     EKYKVELAEFANSGLDSNVLLKENAEGEKVLAPLEGRFFLTKSASDIPLKIGSQVKKG
                     DIVCYVEAMKTYNAIQAETDGVIVDFVVANGDNVSEDDVLLIIQ"
     gene            2106..3338
                     /locus_tag="PLJ82_07575"
     CDS             2106..3338
                     /locus_tag="PLJ82_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOR41398.1"
                     /translation="MEILGNLYEMTAFSNIIAQPQFLLMYAIAFLLLYLGIKKEFEPL
                     LLIPIAFGVLIANFPGGQMGIIQANSEGLVEVGGEMKSIWQMPLHDIAHEMGIMNFLY
                     YMLIKSGFLPPIIFMGVGALTDFGPMLRNIRLSIFGAAAQLGIFTVLIGAILLGFTPK
                     EAAALGIIGGADGPTAIFTTIKLAPHLLGPIAIAAYSYMALVPVIIPLVVKLLCTEKE
                     LRINMREEEKKYPSKTQIKNMRVTKIIFPIAVTTIVALFVPSSVPLVGLLMFGNLLKE
                     VGTNTLRLSDAASNAILNTATIFLGLCVGATMTTEAFLNWQTIGIIIGGFLAFALSIS
                     GGILFVKLINLFSVKKINPLIGATGLSAVPMASRVANEIALRYDKKNHLLQYCMSSNI
                     AGVIGSTVAAGVLISFLS"
     gene            complement(3415..>3960)
                     /gene="eno"
                     /locus_tag="PLJ82_07580"
     CDS             complement(3415..>3960)
                     /gene="eno"
                     /locus_tag="PLJ82_07580"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842055.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOR41399.1"
                     /translation="ASEFYKNGIYDYSKFEGPNGKKRTSAEQADYLAELIANYPIDSI
                     EDGMGEGDWDGWKLLTDKIGSKCQLVGDDLFVTNVEYLKKGIDLGCANSILIKVNQIG
                     SLSETLDAIEMAHRAGYTSVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAK
                     YNQLLRIEEELGDLAVYGYKK"
BASE COUNT         1191 a          579 c          879 g         1311 t
ORIGIN      
        1 agtgaaggat tgttgttgct cttagttggg ttattgtctg tttttgttgt attattagta
       61 gtaatgggta ttagtagatg gcttatttta ttagtaaatc gttattttcc agaaacaaca
      121 tcccgagtgg ataataaatt aattcaagag ccaaaagaac atgtgtcttc atcagattct
      181 atggttattg atgaggttat taaaaaactg tcacaaggaa cttttgttgt caagaaaatt
      241 gaaaaaatca agtaaatagt atgaaaaaag aagttaagtt ttcgttggtt tatcgagata
      301 tgtggcaaag tgcaggaaag tatatgccac gtgtggacca gttggtacga gtagctcctg
      361 aaataattcg tatgggttgt tttgcacgtg tcgaaacgaa tggaggtgga ttcgagcaaa
      421 ttaatttgtt gtttggggaa aatccaaatg tggctgttag gcagtggtgc aagccattta
      481 atgatgcagg tattcaaact cacatgcttg ataggtcttt aaatgcgttg cgtatgtatc
      541 cagtgtcgga agatgtgaga agattattct ataaggttaa aaaagcacaa ggtacagata
      601 tagcacgttc attttgtggg ttaaacgata cccgaaatat tatagcatct attcgatatg
      661 ccaaagaagc aggtatgata gcacaagctg cattgtcgct tactgtgtct cctgtgcata
      721 cggttgatta ttattgtgca atggcagatg aacttatagc taatggtgcc gatgaaattt
      781 gtttaaaaga tatggcgggc attggtcgcc ctgtatcatt aggaaaaata gtagcatata
      841 taaagaaaaa atacccaact attattgtgc aatatcatgg acagactggt cctggtttta
      901 ctccggcttc tattttggag gtttgtaaag ctggttgtga tattattgac gttgggatgg
      961 agcctttatc gtggggtact ggtcatgcag atgtaattat ggtgcgagaa atgttgaaag
     1021 atgctggttt tagtgtgcca gaaatcaata tgtcagctta tatgaaagtg cgtgcattga
     1081 ctcaagagtt tatggatgat tttttaggat attttattcc cgaaacgaat agacatttgc
     1141 aatcgctgtt gatagcacct ggattgccag gcggtatgat gggtagtttg atgaatgatt
     1201 tgaaagataa tttagtgtca ataaacaaag catatcaaaa gcaaggtaaa actaccatta
     1261 atacagatga attgttgatt caactatttg atgaagtggc ttatgtgtgg cctcgcgttg
     1321 gctatcctcc tttggtaacg ccatattctc agtatgttaa aaatttagcg atgtttaata
     1381 tcattcaaat ggctaaaggg aaagctcgtt ggacaatgat atctgatgat acttgggata
     1441 tgatacttgg taaagctgga cgattgccag gagaattggc tcctgagatt aaagagttag
     1501 ctacacagca aggtcgtaca ttttatgaag gagaccctca aattttgtat ccgaatgtgt
     1561 tagaaaattt tcgcaaggaa atgattgata atggatggga tatgggaaaa gatgaggaag
     1621 aattgttaga acttgccatg catccagaac aatatagaac gtataaatcg ggtaaagcta
     1681 aacatgattt tgaaatagag ttagcacaag caaaaaaaga atcgcaacaa cgaggagatg
     1741 gttctttcgt attgccacat gttcttgaaa tgaccgtaaa tggagaaaaa tataaagtag
     1801 aacttgcaga atttgcgaat agtggattag attcgaatgt tcttctaaaa gaaaatgcag
     1861 aaggagaaaa ggtgttggct cctttagaag ggaggttctt tttgacaaaa tcagcctcag
     1921 atattccctt gaaaattgga tcacaagtta agaaagggga tattgtttgt tatgtagagg
     1981 caatgaaaac atataatgct attcaagctg aaactgatgg tgtaattgtt gactttgtag
     2041 ttgctaatgg agataatgtg tcagaggacg atgtgttgct tattattcaa taatcttaat
     2101 gatatatgga aattttagga aatttgtatg aaatgacagc gtttagtaat attattgctc
     2161 aaccgcaatt tttactcatg tatgccattg catttttatt gttgtattta ggtataaaaa
     2221 aggaatttga gccattattg ttgattccga tagcctttgg ggtattaata gccaatttcc
     2281 caggtgggca aatggggatt atacaggcta actcagaagg attggtagaa gtgggtggtg
     2341 aaatgaaaag tatttggcaa atgccgttgc atgatattgc acacgagatg ggaattatga
     2401 attttttata ctatatgctt attaaatcag ggttccttcc tccaataatt tttatgggag
     2461 taggagcttt aacggatttt ggtccgatgt tgcgaaatat ccgattatcc atttttggag
     2521 ctgctgcaca attgggtatt tttacggtat tgataggggc tattttgctt ggttttacgc
     2581 ccaaagaggc tgctgcattg gggataattg gtggagcaga tggacctaca gcgattttta
     2641 caactataaa gttggctcca catttattgg gtcccattgc cattgctgcc tattcgtata
     2701 tggcgttagt ccctgttatt atacctcttg ttgttaagtt gctgtgtact gaaaaagaat
     2761 tgcgtattaa tatgcgtgaa gaagaaaaaa aatatccttc aaaaacgcag attaaaaata
     2821 tgagggtaac aaagattatt ttcccgatag ctgtaactac tattgtagca ttgtttgtgc
     2881 cttcttcggt tccgttagtt ggattgctaa tgtttggtaa cttactaaaa gaagttggta
     2941 caaatacttt gcgactgtca gacgcagctt caaatgctat tttaaataca gcaactattt
     3001 ttttgggctt gtgtgtaggt gctacaatga caacagaagc ttttcttaat tggcaaacaa
     3061 tagggattat tattggcggt ttcttggcgt ttgcgttgtc tatttcaggt ggtattttgt
     3121 ttgttaagct tataaatctt ttttctgtca aaaagattaa cccccttatt ggagctactg
     3181 gcttaagtgc tgtgccaatg gcttcgcgtg ttgccaacga gattgcgtta cgttatgata
     3241 agaaaaacca ccttttacaa tattgtatgt cgagtaatat tgccggtgta ataggttcta
     3301 cagtagctgc tggagttctt atttcatttt tgtcgtaatc tacacttaat gtgtataaaa
     3361 ataataaagc ctagcgaatt tcgctaggct ttatttagta tactgtaagt aggtttactt
     3421 tttgtagcca tatacagcta agtcacctaa ttcttcttcg atacgaagta gttggttgta
     3481 tttagccata cggtcagatc ggctcaacga acctgttttg atttgaccag aatttgtagc
     3541 taccgcaata tctgcaatag ttgcatcttc agtttcgcca gagcggtgag aagtaacgga
     3601 agtatagcca gcacggtgag ccatttcgat agcgtctaat gtttcagaaa gagatccgat
     3661 ttggttaact ttaataagga tagagttagc acaacccaaa tcgatacctt ttttcaagta
     3721 ttctacgtta gttacaaata agtcatcacc tactaattga catttactac caattttgtc
     3781 tgttaatagt ttccatccgt cccagtcgcc ttcacccata ccatcttcga ttgagtcaat
     3841 aggatagtta gctattaatt ctgctaaata gtctgcttgt tcagcagagg tacgtttttt
     3901 tccattagga ccttcaaatt ttgaatagtc atagatgcca tttttgtaaa attcagaagc
//
LOCUS       DAOSRP010000129         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_21210_length_3864_cov_5.272250, whole genome shotgun sequence.
ACCESSION   DAOSRP010000129 DAOSRP010000000
VERSION     DAOSRP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21210_length_3864_cov_5.272250"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLJ82_07585"
     CDS             <1..363
                     /locus_tag="PLJ82_07585"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOR41400.1"
                     /translation="FISSAKASESWLISPSIDLSDATAPKLTFEHTGKYFTSNKLTEQ
                     TLWVSTNYTEGAPSTATWTQLTIPTWPSGSDWTFVSSGAIDLSAYTANSNVRLAFKYA
                     STTAGAATWEFKNLVVKE"
     gene            complement(471..1142)
                     /locus_tag="PLJ82_07590"
     CDS             complement(471..1142)
                     /locus_tag="PLJ82_07590"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOR41401.1"
                     /translation="MKKILFLTSVLVVWQLSLTAQNFYEFKELPYSYSALEPYIDSTT
                     METHYAKHHKGYYNNFVKAMQEGNFDNKPLEQVIQNISSYSVFIRNNGGGYWNHEFFW
                     NCMSPNKTQASNALTKAITDNFGSFENFKKEFSSAASTVFGSGWAWLIVTDSGKLKIV
                     KTANQDNPLMDAVADKGTPILAIDVWEHAYYLKHKNLRAAYVENFWQVINWQFVSEKY
                     ADAVK"
     gene            complement(1368..3275)
                     /gene="dnaK"
                     /locus_tag="PLJ82_07595"
     CDS             complement(1368..3275)
                     /gene="dnaK"
                     /locus_tag="PLJ82_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOR41402.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIANSEGKRTTPSIVAFID
                     GGERKVGDPAKRQAITNPQKTIFSIKRFMGETYAEVSKEIGRMPYKVVKGDNNTPRVD
                     IDGRLYTPQEISAMVLQKMKKTAEDYLGSEVTDAVITVPAYFSDAQRQATKEAGEIAG
                     LNVKRIVNEPTAAALAYGLDKSSKDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDQVIIDWLAEEFTREEGIDLRKDPMALQRLKEAAEKAKIELSSSASTEIN
                     LPYIMPVNGIPKHLVKTLSRAKFEQLADKLIQATVEPCQKAMKDAGLSTSDIDEVILV
                     GGSTRIPAIQDKVQQIFGKTPSKGVNPDEVVALGAAIQGGVLTGEVKDILLLDVTPLS
                     LGIETMGGVMTKLIESNTTIPTKKSETFTTAADNQPSVQIHVLQGERPMASQNKSIGQ
                     FNLDGIPAAQRGVPQIEVTFDIDANGILKVSAKDKATGKEQSIRIEASSGLSDDEIKR
                     MKAEAEANAEADKKEKERIDKLNQADSLIFQTEKQLKDFGDKLPADKKAPIEAALNKL
                     KEAHKTQNLDGIDAATKELNDVFQAASQEMYNAQAQQGGNAQPNAEPQANNQGNDGKD
                     TVTDVDFEEVK"
     gene            3519..>3864
                     /locus_tag="PLJ82_07600"
     CDS             3519..>3864
                     /locus_tag="PLJ82_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR41403.1"
                     /translation="MQKCEPEESLWSKSFVLLLIGSFLLFFAFYLLMPILPMFLEEDL
                     LADKASVGFALFVYSVTALVVRPFAGYMVDNFARRQLLLVSYAAFVSIFVFYVLVQSL
                     SGFVWVRALHGLV"
BASE COUNT         1015 a          704 c          723 g         1422 t
ORIGIN      
        1 tttatcagtt ctgctaaagc atcagagtca tggttaattt cgccaagtat tgatttgtca
       61 gatgctacag ctcctaaatt gacatttgaa cacactggaa aatattttac atcgaacaaa
      121 cttacagaac aaaccctatg ggtatctacc aattacacag aaggagctcc ttctacagct
      181 acttggacac agctaacaat accaacatgg ccaagtggta gtgattggac gtttgtatct
      241 tctggagcaa ttgatttgtc agcttatact gccaatagca atgtgcgttt agcctttaag
      301 tatgcaagta caacagctgg tgctgctacg tgggagttta aaaatttggt tgtaaaagaa
      361 taatagaact atttgttaga tagtacaaaa agagggtgaa tcgtgcgatt catcctcttt
      421 ttattgagta attaatattc agtttatcaa tttgcggtta tatagatgtg ctattttact
      481 gcatccgcat atttttctga tacaaattgc caattgataa cttgccagaa attttcaaca
      541 tacgctgcac gtaggttttt atgtttcaaa taataggcgt gctcccatac atcaatggct
      601 aagatgggtg ttcctttatc tgcaacagca tccatcaatg gattgtcttg gttagcggtt
      661 tttacaattt tcagtttgcc actatctgtt acgataagcc atgcccatcc cgaaccaaaa
      721 acggtagatg ctgccgaaga gaattctttt ttgaaattct caaacgaacc gaaattgtct
      781 gtaatggctt ttgtgagtgc attgctggct tgtgttttgt ttggcgacat acaattccag
      841 aaaaactcat gattccaata tccccctccg ttgttacgaa taaatacact gtaagaggaa
      901 atgttttgta ttacttgttc gagaggttta ttgtcaaaat tgccctcttg catcgctttt
      961 acaaagttgt tgtagtatcc tttgtggtgt ttagcataat gcgtttccat ggtggtggag
     1021 tcgatataag gctctaatgc actatacgaa tagggtagtt ctttaaattc atagaaattt
     1081 tgagctgtaa gtgatagttg ccatacaact agtacactcg taaggaatag tatttttttc
     1141 atatttttat tgtttaattt tacaaatata aacccaaaat gccctttcgt aattttagtt
     1201 aacaagtttt agtgatatgc aatgaatata aacagaagag tttcttgtta atcgtagaga
     1261 aaaagctgct tgtgggattt ttcaattcat tattttcttt ggtggtagat ataaaaaaag
     1321 ctgtaaataa attttaattt acagcttttt taggatgaaa gtttagctta ctttacttct
     1381 tcaaagtcaa catcagtaac agtgtctttg ccatcgttac cttggttgtt agcttgaggc
     1441 tcagcgttag gttgtgcatt tcccccttgt tgtgcttgag cattgtacat ttcttggcta
     1501 gcagcttgga acacgtcgtt aagttctttt gtagctgcat caataccgtc taagttttgg
     1561 gttttgtgag cttctttaag tttattaaga gctgcttcga taggtgcttt tttatcagca
     1621 ggcaatttat ctccaaagtc ttttagctgt ttttctgttt gaaaaatcaa actatctgct
     1681 tggttcagtt tatcgatgcg ttctttctct tttttatccg cttcggcatt ggcttctgct
     1741 tctgctttca tgcgtttgat ttcgtcgtcg ctcaaaccgc tagatgcctc aatacgaatg
     1801 ctttgttcct tgccagttgc tttgtctttt gccgacactt ttaaaatacc atttgcgtcg
     1861 atatcaaaag taacttcaat ttgtggtacg ccacgttgag ctgctggtat gccatcaaga
     1921 ttaaactgtc cgatagattt attttgcgaa gccattggtc gttcgccttg aagcacatgg
     1981 atttgtacag aaggttggtt gtcagcagct gttgtaaatg tttctgattt cttggttgga
     2041 attgttgtgt tcgactcaat caattttgtc attacaccac ccatggtttc gatacctaat
     2101 gataatgggg taacatccaa taatagaata tcttttactt cgccagttaa tacacctcct
     2161 tgaatagccg cacccaatgc tactacttca tctggattta cacctttact tggtgttttg
     2221 ccaaaaattt gttgtacttt atcttgaata gcaggtatac gagtagaacc acccactaaa
     2281 ataacttcat caatatcaga cgtgctcaat ccagcatctt tcattgcttt ttggcaaggc
     2341 tctacagtag cttgaattaa tttatcggct agttgttcaa attttgccct gctcaatgtt
     2401 ttaactaagt gttttggaat accatttaca ggcataatgt atggcaaatt gatttctgtt
     2461 gatgctgagc ttgataattc aattttagct ttctctgcgg cttcttttaa gcgttgtaat
     2521 gccataggat ctttacgtaa atcaatacct tcctcacgcg taaattcttc agccaaccag
     2581 tcgataatta cttggtcaaa atcatctcca cccaagtgtg tatctccgtt agtagatttt
     2641 acttcgaata caccatcgcc caattcaaga atagaaatat caaatgtacc gccacctaag
     2701 tcgaatactg caattttcat gtccttgctt gatttgtcca atccataagc taaggctgct
     2761 gctgttggtt cgttgacaat acgttttacg tttaagcctg caatttcgcc agcttcttta
     2821 gtagcttgac gctgtgcatc gctaaagtag gcaggtaccg taattacagc gtctgtaact
     2881 tcagagccaa gataatcttc ggctgttttt ttcatttttt gcaataccat tgccgaaatt
     2941 tcttgtggcg tatacagtct gccatcaata tctacacggg gcgtgttatt gtctcctttt
     3001 actactttat agggcatacg tccaatttct ttgcttactt cagcgtatgt ttcacccata
     3061 aaacgtttaa tcgaaaagat tgttttttga gggtttgtaa tagcttgacg tttagcggga
     3121 tcgcccactt tacgttcgcc tccatcgata aatgctacaa tcgaaggagt ggtacgtttt
     3181 ccttcgctat ttgcaattac aactggttcg tttccttcca ttacagatac gcacgagttg
     3241 gttgtaccta agtcaattcc aataattttt cccataatgt ttttttgtta aatatgttat
     3301 cgtgttactg attctttttt atattacatc aatgttataa caaaacgtat gccaacattc
     3361 gtttttactg tataaacttt atttatgcat tacaaaactg acaaaagggc aggtttttgt
     3421 taattattct ttacatacaa tacaataaaa ggggagcaag ttgtgcaatg tttaattctt
     3481 ttataccttt gctctctatc caattagtaa tccattatat gcaaaagtgt gaaccagagg
     3541 aatcattatg gagcaaaagt tttgttctat tgttgatagg tagttttttg ttgttttttg
     3601 ctttctattt gttaatgcca attttaccga tgtttttgga ggaagattta ttggcagata
     3661 aagcgagtgt tgggtttgct ttgtttgtat attctgttac agcattggta gtacgaccgt
     3721 ttgctgggta tatggttgat aattttgctc gtagacaatt attgctggta tcgtatgctg
     3781 cttttgtcag tatttttgtt ttctatgtat tggtacaatc gctaagcggt tttgtgtggg
     3841 tacgagcttt gcatggattg gttt
//
LOCUS       DAOSRP010000130         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_21826_length_3779_cov_4.923469, whole genome shotgun sequence.
ACCESSION   DAOSRP010000130 DAOSRP010000000
VERSION     DAOSRP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21826_length_3779_cov_4.923469"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2634
                     /locus_tag="PLJ82_07605"
     CDS             <1..2634
                     /locus_tag="PLJ82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HOR41404.1"
                     /translation="NMMVIASETGVLPFEASEIKEKGRLQPGKMILIDTEKKEIYRDK
                     EIKTELASMFPYGEWLSKNRIKLDQISSGRHPKNDVPNFQQLLRLFGYHKEDVEAILT
                     PMAQDGNEPVSSMGNDTPLAPFSSKQQLFYNYFRQQFAQVTNPPIDPIREELVMATSS
                     YMGVINHNLLQPTPELCKMVQISSPILTNQHIDILRNLSYKGFRTITLDAIYTLQNGV
                     DGLEQAINNLCQAAEKAVDEDYNYIILSDKAVNGTQAPIPSLLSVAAVHNYLIDKRKR
                     VQTALVVETADAREVMHMALLIGYGASSINPYMAYAVINDLVKSKTIQLDYHTAEKNY
                     IKSLNKGLKKVISKMGISTIRSYRGAQLFEALGISSELLSKYLHGTITKLEGIGLEDI
                     HRDIVACHQQAFASELELLSNSGQYAYRRNGEYHAWNPETIAKLQIATRTGDYAKFKE
                     YTRHVDEKEQTVFIRDLIDYKRNPIDISLVEPIENITSGYRMDISHGFCGSDCSNRSD
                     IMFDYYNNGVKAGNEQAYFILEHWEFGDGERENYNQWGMNCHLNNTNSYMQTAMGWLK
                     DGDGLDASNQDGFISYCESHDEERTMFKAKSYGNGATLRTLAGYCNRVPLNTAFNVLL
                     NGSHMMWMFQELGYDYSIFSNAQGGTGDRVDPKPIPESLNWYTNTTRMNAYQKVGQLI
                     QLRTRILPHVFAGDPTNSDLGSGKAARSVIWGSGNDRVFVVGNFNAPSDGETYTGSVN
                     INLPSGNSWYDYLANGSTAMNAGTSVTLQPGDLKIYTAARQTLPTVPNSYSSFPTGGI
                     DIVRDNTVECNVYPTIATDFISIDSAEDIQDVYVVNMKGYTYPRTLMNNSIRVSDLEQ
                     GQYLLIVTFAKEQQAYRFVKK"
     gene            complement(2691..3320)
                     /locus_tag="PLJ82_07610"
     CDS             complement(2691..3320)
                     /locus_tag="PLJ82_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOR41405.1"
                     /translation="MGKNKFYVVWRGRETGVFATWELCKRQVHGFEGAQYKSFDTEKE
                     AQLAFQQPFITQIIRKKIVAKPINAKQHGGVPLQDSLAVDAACSGNPGDMEYRGVYVQ
                     TGQEVFRKGVYSNATNNIGEFLALVHGLAFLKLHNSSIPIYSDSVNAIKWVEKKQCKT
                     KLVKTKNNEEVFELIARAEKWLQENTYITPILKWETDVWGEIPADFGRK"
     gene            3477..>3779
                     /locus_tag="PLJ82_07615"
     CDS             3477..>3779
                     /locus_tag="PLJ82_07615"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOR41406.1"
                     /translation="MVKRIILSIAFITFIQFANANEGMWILPSIPDSIAKNMDKLGYE
                     LKMRDLYNEKKSSLKDVVVSFSNGYTGVMISSDGMLLTNYTAIMPFIEQLDESSQYK"
BASE COUNT         1279 a          669 c          677 g         1154 t
ORIGIN      
        1 aatatgatgg taattgcatc agaaacaggc gtattaccat ttgaagctag tgaaattaaa
       61 gaaaaaggac gtttgcaacc aggcaaaatg attctgatag atacagaaaa gaaagaaatt
      121 taccgagaca aagaaattaa aactgaattg gcatctatgt ttccatatgg tgaatggcta
      181 tctaaaaata gaataaaact agaccaaata agttctgggc gtcatccaaa aaatgatgta
      241 ccaaattttc aacaattatt gcgtctattt ggctatcaca aagaagatgt ggaagcaata
      301 ttaactccta tggcacaaga tggaaatgaa cctgtcagtt ctatgggaaa tgatacgcca
      361 ctagctcctt tttcgtctaa gcaacaactt ttttacaatt atttcagaca acaatttgca
      421 caagtaacca accctcctat tgaccctatt agggaagaac tggtaatggc tactagcagt
      481 tatatgggag taattaacca taacttattg caaccaacac ctgaattatg taaaatggta
      541 caaatcagta gtccaattct aacgaatcaa catattgaca ttttacgtaa tttatcgtat
      601 aaaggtttta gaaccattac gttagatgcc atatacacct tgcaaaatgg agtagatgga
      661 ctagaacagg ctattaacaa tttgtgtcaa gcagccgaaa aagctgttga tgaagattac
      721 aattatatta tactttctga taaagcagta aacggtacgc aagcacctat accatcatta
      781 ttatcagtag ctgctgtaca taattattta atagacaaac gcaaacgcgt acaaacagct
      841 ttagttgtag aaacagcaga tgcacgtgaa gttatgcaca tggctttatt aattggctat
      901 ggagcaagct ctattaatcc atatatggct tatgccgtaa taaacgattt ggtaaagagc
      961 aaaactattc aattagatta ccatacagct gaaaaaaatt acataaagtc tttaaataaa
     1021 gggctgaaaa aagtaatttc taaaatgggt atttctacaa ttcgtagtta ccgtggagca
     1081 caattgtttg aagctttagg tatttcgtct gagttgctta gcaaatatct acatggaact
     1141 atcacaaaat tagaaggcat tggattagaa gatattcatc gagatatcgt agcgtgtcat
     1201 caacaagctt ttgcatctga attagaatta ctttctaata gtggtcaata cgcatacaga
     1261 cgcaatggag aataccacgc ttggaatcct gaaaccattg ccaagttaca aatagcaacc
     1321 cgcacaggtg attatgctaa atttaaggaa tatacgcgtc atgtggacga aaaagaacaa
     1381 acagtgttta ttcgtgactt gattgattat aaacgtaatc ctatagatat ttctttggta
     1441 gaacctatcg aaaatattac tagtggttat cgtatggata tttcgcatgg tttttgtggc
     1501 tctgattgta gcaaccgttc agatattatg tttgattatt acaacaatgg tgtgaaagca
     1561 ggaaacgaac aagcttattt tattcttgaa cattgggaat ttggagatgg agaacgcgaa
     1621 aactacaacc aatggggcat gaattgccat ctaaacaata cgaatagtta tatgcaaaca
     1681 gctatgggtt ggttgaaaga tggagatgga ctagacgcat caaaccaaga cggatttatt
     1741 tcttattgcg agagccatga cgaagaacgc actatgttta aagcaaaatc gtatggtaat
     1801 ggtgccactt tacgtacttt agcgggctat tgcaatcgtg tacccttaaa tacagcattt
     1861 aatgtcctat taaatggttc gcatatgatg tggatgttcc aagaattagg ctatgattat
     1921 agtatattta gcaatgctca aggagggaca ggagatcgtg ttgaccctaa accaataccc
     1981 gaaagtttaa attggtatac gaacactacc cgtatgaatg cataccaaaa agtgggacaa
     2041 ttaattcaac tacgtacgcg tattttacca cacgtatttg ctggcgaccc tactaactcc
     2101 gatttaggaa gtggcaaggc tgctcgtagt gtcatttggg gatctggcaa tgaccgtgta
     2161 tttgtggtag gaaactttaa tgctcctagc gatggtgaaa cgtatactgg atcggttaac
     2221 atcaatttac cttctggaaa tagttggtat gactacctag ccaatggttc tactgctatg
     2281 aatgctggta cttctgtaac attacaacca ggagacttaa aaatttatac tgcagctcgt
     2341 cagactttgc caactgtacc aaactcatat agtagttttc caacaggagg tattgatatc
     2401 gtacgggata atacagtaga atgcaatgtt tatcctacaa ttgctaccga ttttattagc
     2461 attgattcag cagaagatat acaagacgta tatgttgtaa atatgaaagg atacacctat
     2521 ccacgtacct taatgaacaa tagtatcaga gtatctgacc ttgaacaagg acaatacttg
     2581 ctaattgtaa cgtttgctaa agaacaacaa gcataccgtt ttgtaaaaaa ataaacaaag
     2641 agaaatactc taaacctaaa agaagtgcct tgaaaatcga ggcacttctt ttattttcgt
     2701 ccaaaatcag caggaatttc accccacaca tccgtttccc acttcaggat tggggtaata
     2761 taggtatttt cttgaagcca tttttctgcc cgtgctatta actcgaagac ttcttcgttg
     2821 tttttggttt taactaattt agttttacat tgtttctttt ccacccattt aatagcattg
     2881 acactatcag aataaatagg aatagaacta ttgtgcagtt ttagaaaagc taatccatgt
     2941 actaaagcca gaaactcgcc aatattattg gtcgcattac tgtaaacacc ttttctaaac
     3001 acttcttgac ctgtctgcac atacacgcca cgatactcca tatcgcctgg attaccactg
     3061 catgctgcat ctacagccaa actatcttgt agagggacac ctccatgttg tttggcatta
     3121 attggtttag caactatttt tttccttata atctgtgtaa taaaaggttg ttgaaatgct
     3181 aattgagctt ctttttctgt atcaaatgat ttgtactgag ctccctcaaa accatgtacc
     3241 tgccgtttac acaattccca agtagcaaac accccagttt ctctacctct ccatactacg
     3301 taaaatttat tttttcccat atgtgcaata tactcctgca aaaatacatt ttttagatga
     3361 atatgcaatg cgtgtgtagt gtattagatt gagctatacg ataacttatt ttatctatac
     3421 taaattaatt ttgtatattt gcaggtaatt agcaacctta tagcaataaa aataatatgg
     3481 taaaaagaat aatcttaagt atagctttta ttacattcat acagtttgcc aatgccaacg
     3541 aaggtatgtg gatattacca tccataccag actccattgc taagaacatg gataaactgg
     3601 gatatgaatt aaaaatgcgt gatttatata atgaaaaaaa atcttcttta aaagatgtag
     3661 tcgtttcatt ttcaaatgga tacacaggcg taatgatttc ttcggacgga atgttgctta
     3721 ctaattacac agcgattatg ccatttattg agcagttaga tgaatcctct caatacaaa
//
LOCUS       DAOSRP010000131         3742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_22088_length_3742_cov_5.094386, whole genome shotgun sequence.
ACCESSION   DAOSRP010000131 DAOSRP010000000
VERSION     DAOSRP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22088_length_3742_cov_5.094386"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLJ82_07620"
     CDS             <1..1464
                     /locus_tag="PLJ82_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR41407.1"
                     /translation="ILNHAEITFIFLEGEELLRKIEHIVPEIPTLKGIYTFVNRGRFH
                     YFEQLINLGKENLEIDKINAIKATITPNDVACMIYTSGTTGNPKGVMLSHQNITMQLK
                     MLQNTPAKWSDTAFSFLPICHAYEKMIVYLYQYLGMSVYYAESLATISENIKEVHPTM
                     MTCVPRMLEKIFDKLESAGRKLPFFQQKLYFWAINVAFDYNIENRTLWYTVKHKIADK
                     LIYSKWRQAIGGNFDIVVSGGAAIQKRLASFFSAIGMPVFEGYGLTETSPVIAVSSRA
                     KYGRCAGTVGFPLEGVEVKILPVTNEIVCRGHNVMLGYYKDEALTKEVIDEDGWFHTG
                     DTGKFTEKGQLVITGRLKNIFKTSFGKYVNSFLIEEKFVQSPFIENIVVVGENQKFAA
                     AIIIPDFTFLKLWCERYHIPYSTNEEMLQNEDVKNRFKKEIKKYNSHFGDYEQIKKFE
                     LIADEWTQQNGILTPTLKVKRSIILERYKPIIDKLFL"
     gene            1632..2222
                     /locus_tag="PLJ82_07625"
     CDS             1632..2222
                     /locus_tag="PLJ82_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOR41408.1"
                     /translation="MKTLKKLTDDELVELYVSGNNPAFEVLLLRHKNKVYAYIYNMVR
                     DHDMSDDIFQETFIKVITCIRTESYTANGKFCSWVTRIAHNLCIDALRCNQKNNTVSA
                     DNTEFNVLNSISLCDKNVEDNLIEEQTFNEIERLVNMLPTNQQEIIKMRFYNDCSFKD
                     IAEKTGVSINTSLGRMRYALLNMRKLAEENNIILSF"
     gene            2275..2460
                     /locus_tag="PLJ82_07630"
     CDS             2275..2460
                     /locus_tag="PLJ82_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41409.1"
                     /translation="MNNLRRVPMNHFTSHLQKKEVSQENNLMQPKAETVNKILMFAAC
                     YHVQKIDNGSFFEIILN"
     gene            2523..3173
                     /gene="rpe"
                     /locus_tag="PLJ82_07635"
     CDS             2523..3173
                     /gene="rpe"
                     /locus_tag="PLJ82_07635"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOR41410.1"
                     /translation="MATLIAPSLLAANFAHLQKDIEMINQSTADWLHLDIMDGVFVPN
                     ISFGFPVIESIKDICQKPLDVHLMIVEPDKFIADFATVGTYMLNVHYEACTHLHRTIQ
                     KIKAANMKAAVTLNPHTPICVLQDIIQDVDMVLLMSVNPGYGGQKFITNTLQKTADLK
                     ELILKKNAKALIQIDGGVNLTTGRQLVEQGADVLVAGSFVFASTNPLQTIAELKKL"
     gene            3183..>3742
                     /locus_tag="PLJ82_07640"
     CDS             3183..>3742
                     /locus_tag="PLJ82_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOR41411.1"
                     /translation="MSSTPLFHQIPFFRLTSALVIGIFISSVVPLSLTIGYILLFLSI
                     IGVVISNVIDKRKTLSYSFRWIYGVSIIGIFMSLGMLLYQHKIQELSLELPDNKKTFI
                     ATISESPIEKPKSVMTVVTIAVPEINKTHRAILYLEKDSASKTVQYGDYLLVNTLLQK
                     PRNTGNPYEFDYSTFLLHKGITATSY"
BASE COUNT         1364 a          584 c          621 g         1173 t
ORIGIN      
        1 attttaaacc atgccgaaat aacgtttata tttttagaag gagaagaatt attacgcaaa
       61 atagaacata ttgttcctga aatacctacg ctaaaaggaa tttatacatt tgtaaacaga
      121 gggcgttttc attattttga gcaattaata aacttaggaa aggaaaatct cgaaattgat
      181 aaaatcaatg caataaaagc aacaattacc ccgaatgacg tagcctgtat gatatacact
      241 tctggtacta caggaaatcc taaaggagta atgctaagcc accaaaatat tacaatgcaa
      301 ctcaaaatgt tgcaaaatac accagcgaaa tggagcgata cagcatttag ttttttgcct
      361 atttgtcatg catacgaaaa aatgatagtt tatctctatc aatacttggg aatgtctgta
      421 tattatgccg aaagtttagc taccatatcc gaaaatatta aggaagtaca tcctaccatg
      481 atgacttgcg ttcctcgtat gttggagaaa atatttgaca aactcgaaag tgctggacgt
      541 aaattacctt ttttccaaca aaaactatat ttctgggcaa taaatgttgc ctttgattac
      601 aatattgaaa atcgtacact ttggtataca gtaaaacaca aaattgctga caaattaatt
      661 tatagcaaat ggagacaagc cattggagga aactttgaca ttgtagtgtc tggaggagct
      721 gccatacaaa aacgattggc aagttttttt tcagctatag gtatgcccgt atttgaaggt
      781 tatggattaa cagaaacctc tccggtaata gcagtaagtt cgagagcaaa atatggcaga
      841 tgtgctggaa cagtaggatt tccgttagaa ggagtagaag taaaaatact ccctgtaaca
      901 aatgaaattg tatgtagagg acacaacgtt atgctcggtt attataaaga tgaagctctt
      961 acaaaagaag taattgacga agatggttgg tttcatactg gagatacagg taaatttaca
     1021 gaaaaagggc aattggtaat tactggaaga ttaaaaaaca tctttaaaac ctcttttggt
     1081 aaatatgtaa actccttttt aatagaagaa aaatttgtac aatctccctt tatagaaaat
     1141 attgtcgtag taggcgaaaa tcaaaaattt gctgctgcca ttattattcc cgattttaca
     1201 tttttaaaat tatggtgcga aaggtaccat atcccctatt ctactaatga agaaatgctt
     1261 caaaatgaag atgtaaaaaa tagatttaaa aaagaaataa aaaaatacaa ctctcatttt
     1321 ggtgactatg aacaaattaa aaaatttgaa cttattgcag atgagtggac tcaacaaaat
     1381 ggcattttaa ctcctacact aaaggtaaaa cgttccatta ttcttgaaag atataaaccg
     1441 attattgata aattattttt ataaaggaat aattgtatta cataacaaga tacttattgt
     1501 gtagaaaatt atgttaaata ttgaataaaa ttttgttgtt ttaaatttta ttgttttatt
     1561 tgcagttgta taatccttaa aatcgaaagc ctattgccct attttactct agaacactaa
     1621 aataaagagt aatgaaaaca ctaaagaaac ttactgacga tgagttggtt gagttgtacg
     1681 taagtggcaa taacccagct ttcgaggtgc ttttacttcg tcacaaaaat aaagtttacg
     1741 cttatatata caatatggtg cgtgaccatg atatgagtga tgacattttt caggaaactt
     1801 ttattaaagt aattacttgt atacgtacgg aaagttatac agcaaacggt aaattctgta
     1861 gttgggttac tcgtatagcc cacaacttgt gtatagatgc actacgttgc aatcaaaaaa
     1921 acaataccgt atcagcagat aatacagaat ttaacgtatt aaattctatc tctctttgtg
     1981 ataaaaatgt agaagacaac ctaattgagg agcaaacatt taacgaaata gaacgccttg
     2041 ttaatatgtt acctactaat caacaagaaa taatcaaaat gcgtttttac aacgattgta
     2101 gttttaaaga tattgctgag aaaacaggtg taagcatcaa tacgtcatta ggtagaatga
     2161 gatatgcact acttaatatg cgaaaattag ctgaagaaaa caatattata ctttcatttt
     2221 gaaaaattaa gattattctg tacaataaac tagagattgg tacgttctaa gattatgaac
     2281 aatctaagac gcgtgcctat gaatcatttt acctcccatc tacaaaaaaa agaagtatct
     2341 caagaaaaca accttatgca acccaaggca gaaacggtaa acaaaatact tatgtttgca
     2401 gcatgttacc atgttcaaaa aatagataat ggcagttttt ttgaaattat tctaaactga
     2461 ttggaaaaat cagtgagctt atcatttatt ttatttaatt ttgtagtgta aaaatttaca
     2521 ttatggctac acttattgct ccatcattat tagctgccaa ttttgctcat ttgcaaaaag
     2581 acattgaaat gattaaccaa agtactgctg attggttaca cttggatatt atggatgggg
     2641 tatttgttcc caatatttct tttggctttc ctgttataga aagcattaaa gacatctgcc
     2701 aaaaaccatt agatgtgcat ttaatgattg ttgagccaga taaatttatt gccgattttg
     2761 ctactgtggg cacatatatg ctaaatgttc attatgaagc atgtacacat cttcatcgca
     2821 cgatacaaaa aattaaagca gccaacatga aagcagctgt aacacttaat cctcatactc
     2881 ctatttgtgt tttacaagat attattcaag atgtagatat ggtactattg atgtcagtaa
     2941 atccaggata tggtgggcaa aaatttatta ctaatacctt acagaaaacg gctgatttaa
     3001 aagagttgat actgaaaaaa aacgctaaag ctttgattca aattgatgga ggagtaaatc
     3061 ttactacggg taggcaactc gttgaacaag gtgcggatgt gttagttgct ggaagttttg
     3121 tttttgcctc cactaatcct ttacaaacta ttgctgaatt aaaaaaactt tgatgctagg
     3181 taatgtcatc tactcctttg tttcatcaaa tccctttctt tagacttaca tcagctctcg
     3241 ttatcggcat atttataagt tctgtagttc ccttaagcct cactattggt tacatattgc
     3301 tttttttgtc aatcatcgga gtggttattt ccaacgtaat agacaaaaga aaaacgttga
     3361 gctattcttt tcggtggata tatggtgtat ccattatagg aatatttatg agccttggaa
     3421 tgctcttata ccagcataaa atacaagaat taagccttga acttcctgac aacaaaaaga
     3481 catttattgc cacaattagt gaatctccta tcgaaaagcc aaaatcagta atgacggttg
     3541 ttacaatagc agttccagaa ataaataaaa cacatagagc tatactatac ctagaaaaag
     3601 acagtgcatc gaaaacagta caatatggtg attatctgct agtgaacaca ctactccaaa
     3661 aaccgcgaaa tacaggaaac ccatacgaat ttgattattc aacattttta ttacacaaag
     3721 gcattactgc cacatcatac at
//
LOCUS       DAOSRP010000132         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_22478_length_3694_cov_3.061006, whole genome shotgun sequence.
ACCESSION   DAOSRP010000132 DAOSRP010000000
VERSION     DAOSRP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22478_length_3694_cov_3.061006"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..3328)
                     /locus_tag="PLJ82_07645"
     CDS             complement(278..3328)
                     /locus_tag="PLJ82_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41412.1"
                     /translation="MRNRQHTPTLPYNKSKLKERHSFILYLIPFFVFLFCNSLGLYAQ
                     QASKNEANDVLTAIESSQRYIDKLSINDLRVLPVGMQKTIGNIKVIIAVYDITYHSEY
                     ASMSVYAKMLIPQGDGTELFFGMEDVKLSYDGGIIGDAKLKLLGDITIPFDSNTKLVL
                     KGSFQPKTEIKEDLTYAVLTCDGISEIGVSGEVQFATSFITKVNNETATNEAVIGAFK
                     TVITDWNDLLVNINLPSFAIVGLDDYIFSCNNVVLDFSDTRNAQGMVFPETYKELYLP
                     TGNETLWRGVYATAISMKLPEQFTRSDKPKERISIDAQHLIFDHNGVSGLFATNYAIL
                     PYDKGTASGWNFSVERFRLGMLANRVSEASFGGAIGIPLGEKCKLEYNATIDASNKYH
                     LVVNPANELSFDVFKAKATIEKNSYVSLSLDNKKFKPQAVLHGKMGIQLRDDKSGSET
                     AQFTGIKFQGLKLQTESPYFTIDEFGYEGKSVLANLPLSISNIGLKATDNQAILSFNF
                     GIKLAEVVQANSSLSIVAESTGETNKQTWKYKELIVQDIVVSANIAEVMQLNGRLQLL
                     RNDPIYGNAVGGSLLFALSEGLHGLSIEASAMFGKKDFSYWYVDAKAAFATGIPAGGV
                     TLYGFSGGASYAMSKLPGSTNLQKTTCKYEPSSSMGLGLKAGVLFGIPTKQTLNCEAS
                     FEILFNEHGGLNFIGFYGQATMMGNVPSTGSLDKVSQVQTTLLQKEKAYADKYGIDAL
                     LKLKESNPSKVANEFTTLPSPIEGIMARVSIEYGFTRHTLDAKFDTYVNVAGGVIKGG
                     LSGYKAGEGVLHISPTKWYVYVGRPDRPISLSIGIGGIRANSQSYFMLGDVIPPMPPP
                     PKKVVELLKIPTTNLASNRNGASLATGKGIAFGSNFGFDTGDLKCLLFYARLDTQIGA
                     DVMLTHNNSIKCKGRQGIRGVNGWYGSGQAYTYLYGVFGVEARVFGKSYRLPIVSGSS
                     AVLLQAQLPNPIWLKGDVALQYNVLRGLVRGRGNFELEFGEQCK"
     gene            complement(3331..>3694)
                     /locus_tag="PLJ82_07650"
     CDS             complement(3331..>3694)
                     /locus_tag="PLJ82_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41413.1"
                     /translation="NIIKSATDDTLNKTNWQVSSSFNYTYSDMQQATNKMFGPTVAVS
                     ARFLNKTLQSGVSYSCNWSYTEAEYDQFVTNIRIHSSYTFLKKHTLQASGTYQLLAKN
                     TTPNIYRTNLTIAYRYTF"
BASE COUNT         1070 a          794 c          606 g         1224 t
ORIGIN      
        1 tcagggctaa cgcccctaca ttcgtttttt cgtgatattt tgctaccaag acaacagggc
       61 tacgcccctt tctgtactct caacttttca tcgcaacgaa gatttcgggg ctacgctcct
      121 tatttgacta tcaagcattc aactctaatc attaaacact aaacattaaa cattaaacac
      181 tcatcgctac caagacaaca gggctacgcc cctttctgta cttttaactt tcaacttttc
      241 actatcatct gtcaattatc aactatcaac taatcaatca cttgcactgt tctccaaact
      301 ctaattcaaa attgccacgt cctcttacta aacctcttaa tacattatac tgtagagcca
      361 catctccttt taaccatatt ggatttggta actgtgcttg tagcaatact gccgacgatc
      421 cactcactat gggtaatcga taggacttac cgaatactct tgcttctact ccaaacactc
      481 cataaagata ggtatatgcc tgtccgctgc cataccaacc attgactcct cttatccctt
      541 gtctaccttt gcactttatc gaattattgt gtgttaacat aacatcagca cctatttgcg
      601 tatctaatct tgcataaaac agtaaacatt ttaaatcgcc tgtatcaaat ccaaaattcg
      661 acccaaaagc aatgcctttt cctgtcgcca atgaagcccc atttcgattg gacgcaagat
      721 ttgttgttgg gatttttaat aactccacta ctttcttagg tggtggtggc ataggtggga
      781 taacatctcc taacataaaa taactttgtg aattagcacg gatgcctcct atacctatcg
      841 aaagactgat aggtctatcg ggtcgaccca catacacata ccacttggtt gggctaatat
      901 gcaatacacc ttcacctgct ttatatccag ataatccgcc ttttattacc cctcctgcta
      961 cattgacgta ggtatcgaac ttggcatcta gggtatgtct agtaaatcca tactcaatag
     1021 atacacgtgc cattataccc tcaatgggag atggaagagt tgtaaattca tttgcgactt
     1081 tcgacggatt tgattctttc aattttaaca aagcatctat tccatactta tcggcatacg
     1141 ctttttcttt ttgtaataat gttgtttgta cttggcttac tttatccaat gagcctgttg
     1201 atggaacatt ccccatcatc gtagcttgtc cgtaaaaacc tataaaatta agtcccccat
     1261 gttcattaaa taatatttca aagcttgcct cacaatttaa tgtttgctta gtagggatac
     1321 caaataacac tcccgctttt agtcccaatc ccatgctaga ggatggttcg tatttgcaag
     1381 ttgttttttg tagattagta gaaccaggca atttactcat tgcatacgat gcccctccag
     1441 agaatccgta taaggttact ccaccggctg gtattcctgt agcaaaagcg gctttcgcat
     1501 ctacgtacca atatgaaaaa tcttttttcc cgaacatagc acttgcttca atagataaac
     1561 catgtaatcc ttctgacaag gcaaatagca gactacctcc tacagcattg ccataaattg
     1621 ggtcgtttct aagaagttgg agtcttccgt ttaattgcat cacttcggct atattggcag
     1681 agacaacgat atcctgtact atcaattctt tatatttcca tgtttgcttg ttggtttctc
     1741 ctgtactttc agccactatg ctcaaacttg aattggcttg tactacttcg gctaatttta
     1801 taccaaaatt aaaagataag atagcctgat tatcagttgc tttcaagcct atattgctga
     1861 tagaaagagg taaattagca agtactgatt tcccttcgta gccaaattca tcaatagtaa
     1921 agtaggggct ttctgtttgt agttttaagc cctgaaactt aatgccagta aactgggctg
     1981 tttcacttcc tgatttgtca tctcgcaatt gaatacccat ttttccatgc aaaacggctt
     2041 ggggtttaaa ctttttattg tctaatgaaa ggctaacata cgaattcttc tctattgtag
     2101 ctttcgcttt aaatacatca aagcttagtt cattggcagg gttaaccact aaatgatatt
     2161 tattggaggc atcgatagta gcattatatt ccaacttaca tttttcgcca aggggaattc
     2221 caatagctcc gccaaacgag gcttcggaaa ctctattggc taacatccct aacctaaacc
     2281 gttcgacaga aaaattccag cctgacgccg ttcccttatc atacggcagt atggcatagt
     2341 tagtggcaaa aagaccagat accccattat ggtcaaaaat aagatgttga gcatcaatcg
     2401 aaatacgttc tttgggttta tctgagcgag taaattgttc aggcagtttc atgcttatgg
     2461 cagtagcata cacgcctcgc cataatgttt cattgcctgt aggtaaatat agttctttat
     2521 aggtttcagg gaaaaccata ccttgtgcat tgcgtgtatc gctaaaatct aacactacat
     2581 tattgcaaga aaatatataa tcgtcaagcc caacaattgc aaaacttggc aaattaatat
     2641 tcaccaacaa atcattccaa tcagtaatta cggttttaaa tgcaccaatt actgcctcat
     2701 ttgtagcggt ttcattattc actttggtta taaatgatgt tgcaaactgc acttctccgc
     2761 ttaccccaat ttctgaaata ccatcgcatg ttaaaaccgc gtaagtaagg tcttctttta
     2821 tttctgtttt gggttggaat gagcccttca ataccaattt tgtattacta tcgaacggaa
     2881 tagtaatatc gcccaatagt tttaatttgg catcacctat aattcctcca tcgtatgaca
     2941 atttcacatc ttccatacca aaaaacaact cagtgccatc gccttgtgga ataagcattt
     3001 ttgcatacac actcatcgaa gcatattctg agtggtacgt gatatcatac accgctatga
     3061 ttacttttat gtttccaatg gttttttgca taccaacagg caatactctt aaatcattta
     3121 tggataattt gtcaatatac cgttgtgatg attctattgc cgttagcacg tcattagctt
     3181 cattcttgct agcttgttga gcatataaac ctagcgagtt acaaaaaaga aatacaaaaa
     3241 atggtatcaa atacaatata aaagagtgtc tctctttcaa cttactttta ttgtatggca
     3301 atgtaggggt gtgttgtcta tttctcatag ttaaaatgta tatctataag ctatggttaa
     3361 attggttcta taaatatttg gtgttgtatt tttggctaat aattgatatg tgccacttgc
     3421 ttgtaaagtg tgtttcttga gaaatgtata actcgaatgt atccgaatat tggttacaaa
     3481 ctggtcatac tcagcttctg tgtacgacca attgcacgaa tatgatacac ccgattgaag
     3541 tgttttgttt agaaatcttg ccgaaacagc tacagtagga ccaaacatct tgtttgtagc
     3601 ctgttgcata tctgaatagg tataattaaa cgaactgctt acctgccagt tggttttgtt
     3661 aagcgtgtca tcagttgcag acttgatgat attg
//
LOCUS       DAOSRP010000133         3680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_22597_length_3680_cov_5.420138, whole genome shotgun sequence.
ACCESSION   DAOSRP010000133 DAOSRP010000000
VERSION     DAOSRP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22597_length_3680_cov_5.420138"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PLJ82_07655"
     CDS             <1..960
                     /locus_tag="PLJ82_07655"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOR41414.1"
                     /translation="GCSYAFMEVSSHASDQKRTAGLHFAGGIFTNLTRDHIDYHQTME
                     AYLKAKKTFFDLLPKTAFALTNTDDKNGLVMLQNTAANKYTYSTQTLADFKGKIIELS
                     FEGMLLSINNKELSVAFVGKFNVSNLLAVYGTAVLLGIHPDEALIALSTLKPVSGRFE
                     TLQSPTGFTAIVDYAHTPDALNNVLETINDIRSEGIAENMGKLYTVVGCGGNRDKGKR
                     PIMAKEAVKASNMAIFTSDNPRNENPKDILNDMVAGINEEEKKSTLVIEDRYQAIKTA
                     CMLAQRGDIILVAGKGHETYQEIAGVKHHFDDKEVLAELFAQL"
     gene            974..2200
                     /gene="mraY"
                     /locus_tag="PLJ82_07660"
     CDS             974..2200
                     /gene="mraY"
                     /locus_tag="PLJ82_07660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOR41415.1"
                     /translation="MLYYLFRFLEENFNLPGAGLFSYVSFRAGMALIFSLFISMVIGK
                     NIINYLRKKQIGESIRDLGLDGQMSKKGTPTMGGIIIIIAILVPSLLFGRIRNIYLLL
                     MIATTVWLGVIGFLDDYIKVFRKHKEGLNGKFKIVGQIGIGLLVGMTMFLSPEVVMIE
                     NTNHVKVQHIETTELDSKSIAVKSTQTTIPFIKNTNFDYADFLPFLKEHTQTGAWILF
                     IIITIFIVTAVSNGANLTDGLDGLAAGSSSIIGITLGVLAYVSSHVGFAGYFDIMFVP
                     GSEELVVFASAFVGATVGFLWYNSYPAQVFMGDTGSLTIGGIIGVFAILIHKEFLLPI
                     LCGIFLIENLSVMMQVSYFKYTKKKYGEGRRIFLMSPLHHHYQKKGIPEAKIVTRFWI
                     IGIALAIFTLITLKIR"
     gene            2203..3537
                     /gene="murD"
                     /locus_tag="PLJ82_07665"
     CDS             2203..3537
                     /gene="murD"
                     /locus_tag="PLJ82_07665"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOR41416.1"
                     /translation="MKKIVILGGGESGVGAAVLAKKQGFDVFVSDASGIKQKYREVLQ
                     KYAIRWEEGTHTYSEIMDATEVIKSPGIPDKAPLIKEIKARHIPIISEIEFAGRYTKA
                     KTICITGSNGKTTTTMLTYHILQQAGLNVGLAGNVGKSFALQVAENQFDYYVIELSSF
                     QLDNMYTFRADVAVLLNITPDHLDRYDYNFQNYVNAKMRITQNQTENDVFIYWQDDPS
                     INQELTKHTIKAKLYPFSMKKKDNTKSFVDKNNLIINTEETDMSINIDNLAIKGTHNV
                     YNSMAAALSASVMNIKKEQIRNSLADFEGVEHRLEPVAKVRNVMFINDSKATNVNSCW
                     YALESMKTPVVLILGGTDKGNDYNEIKSLVEQKVHTLVFLGIDNSKLEAFFGKLNKKI
                     ISTSSMDDAVKAAYLAANKGDSVLLSPCCASFDLFTNYEDRGTQFKNCVKNL"
BASE COUNT         1311 a          601 c          704 g         1064 t
ORIGIN      
        1 ggatgttcgt atgcatttat ggaagtaagt tcgcacgcta gcgatcaaaa acgcactgct
       61 ggattacatt ttgcaggagg tatttttaca aatcttacac gcgaccatat cgattatcat
      121 caaacaatgg aagcctattt aaaagccaaa aaaacatttt ttgacttgtt gccaaaaacg
      181 gcttttgcac ttacaaatac tgatgataaa aatggattgg tcatgttgca aaatactgct
      241 gcaaacaaat acacctattc aacccaaaca ttagctgact ttaaaggaaa aatcattgag
      301 ctaagctttg agggcatgtt actttctatc aataacaaag agctatcagt agcatttgta
      361 ggcaaattta atgtttcaaa tttactagct gtatacggaa cagcagtatt acttggcata
      421 caccccgacg aagctctcat cgctttgagt acactaaaac ctgtaagcgg tcgttttgaa
      481 acgctacaat ctcctactgg atttacagct attgtagatt atgcacacac acctgatgca
      541 ttgaacaacg tattagaaac aatcaatgat atccgatcag aaggaatcgc tgaaaatatg
      601 ggaaaactat acacagttgt tggttgtggt ggtaatagag ataaaggtaa aagacccata
      661 atggcaaaag aagcagtaaa ggcaagcaac atggcaatct ttacttcaga taaccctcgc
      721 aatgaaaacc ccaaggatat tctgaatgat atggtagcag gaataaatga agaagaaaaa
      781 aaatcaacac tagtaattga agataggtat caagccataa aaacagcctg tatgttagct
      841 caacgtggag atattattct ggtggcagga aaaggacacg aaacgtatca agaaatagca
      901 ggcgtaaaac atcattttga cgacaaagag gtattagcgg aactatttgc tcaactataa
      961 aacaaagtag cgtatgttgt actatttatt tagattttta gaagaaaact ttaacttacc
     1021 tggtgcaggt ttgtttagtt atgtatcatt ccgtgcaggc atggcactca ttttctcgct
     1081 ctttatttcg atggttatcg gaaaaaatat catcaactat ttgcgtaaaa aacaaatagg
     1141 agaatccatt cgtgatttag ggcttgatgg acaaatgagc aaaaaaggaa cacccactat
     1201 gggagggatt attatcatta tagccatctt ggttcctagt ttattgtttg gtcgcatacg
     1261 taatatatat ttacttttga tgatagcaac tacagtatgg ttgggagtta tcggttttct
     1321 cgacgactat attaaagtgt tcagaaaaca caaagaggga ttaaacggaa aattcaaaat
     1381 tgttgggcaa attggcattg gactattagt gggtatgacc atgtttttaa gtcctgaagt
     1441 agtaatgata gaaaatacca atcacgttaa agtacaacat atagaaacaa ccgaattaga
     1501 ctcaaaaagc atagctgtaa aatcgacaca aaccactatt ccatttatca aaaatacaaa
     1561 ctttgattat gctgattttt taccattctt aaaagaacac acacaaacag gagcatggat
     1621 tttatttatt ataattacta tatttattgt aacagcagtg tcaaatggag caaacctaac
     1681 ggatggatta gatggacttg ctgctggttc gtctagtatc attggtatta cactgggcgt
     1741 attagcatat gtatctagcc atgtggggtt tgctgggtat tttgacatca tgtttgtgcc
     1801 tggaagcgag gaattagtag tatttgcaag tgcttttgtg ggagccacag ttggtttttt
     1861 gtggtacaac tcctaccctg ctcaggtatt tatgggcgac acaggaagtt taacaattgg
     1921 aggcattatt ggcgtgtttg cgatactcat acacaaagaa tttttactac caatcttatg
     1981 tggcattttc ttaatagaaa acctgtcggt aatgatgcaa gtatcgtact ttaaatatac
     2041 aaaaaagaaa tatggagaag ggagacgcat atttttaatg tcacctttac atcatcatta
     2101 tcagaaaaaa ggcattcctg aagctaaaat tgtaactcgt ttttggatta ttggtattgc
     2161 actggcaata tttacactta taacacttaa aattcgctaa atatgaaaaa gatagtgata
     2221 cttggtggag gagaaagcgg agtaggtgct gctgtattag caaaaaaaca aggctttgat
     2281 gtatttgttt cagacgcttc tggtatcaaa caaaagtatc gagaagtatt acaaaaatat
     2341 gctattagat gggaagaagg aacacataca tactcagaaa taatggatgc tacagaggtg
     2401 attaaaagcc ctggcatacc agacaaagcc cctcttatta aagaaataaa agctagacac
     2461 atacccatta tttcggagat agagtttgct ggtcgttata caaaagctaa aaccatttgt
     2521 atcacaggaa gcaatggaaa aactaccacc acaatgctaa cctatcacat tttgcaacaa
     2581 gcaggattga atgtgggact agcaggcaac gtgggtaaaa gctttgcact acaagtagca
     2641 gaaaatcagt ttgattatta tgtaattgaa ctaagcagtt ttcaactcga caatatgtat
     2701 actttcagag ccgacgtagc tgtattgtta aacattacac ccgatcattt ggacaggtat
     2761 gactacaatt ttcaaaacta tgtgaatgct aaaatgcgta ttacgcaaaa tcaaacagaa
     2821 aacgatgtat ttatctattg gcaagatgat ccatctatta accaagaact aacgaaacat
     2881 actataaaag caaaactata tccattttct atgaaaaaga aagataacac caaatcattt
     2941 gttgacaaaa ataaccttat aatcaataca gaagaaaccg atatgagcat caacatcgac
     3001 aacttggcaa tcaaaggcac tcacaatgtg tacaattcaa tggcagcggc actatctgct
     3061 agcgtaatga acattaaaaa agagcagata agaaactctt tggcagattt tgaaggagtt
     3121 gaacatcgtc tagaacctgt agcaaaggta cgaaatgtca tgtttattaa cgattcaaaa
     3181 gccaccaatg ttaactcgtg ttggtacgca cttgaaagta tgaaaacacc agtggtatta
     3241 attttaggtg gtacagacaa aggcaatgac tataacgaaa taaaaagctt ggtagagcaa
     3301 aaagtacaca ccctggtttt tttaggtatt gacaatagta aattggaagc gttttttggg
     3361 aaactaaata aaaaaataat tagcaccagt tcgatggatg atgctgtaaa agctgcttat
     3421 ttagcagcca acaaaggaga ttctgtttta ttatcaccct gctgtgccag ttttgattta
     3481 tttactaact acgaagatag aggaacacaa tttaaaaact gtgttaaaaa tttataacaa
     3541 tttgatgtaa gaataattat gggcgaacta tttaaaaaag tatttaaagg agattttatc
     3601 ttatggatta ccataggact tctccttttg gtttcgtcta tcgaaatgtt tagtgctatc
     3661 agtcaagagg cttttagacg
//
LOCUS       DAOSRP010000134         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_22688_length_3669_cov_5.709463, whole genome shotgun sequence.
ACCESSION   DAOSRP010000134 DAOSRP010000000
VERSION     DAOSRP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22688_length_3669_cov_5.709463"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLJ82_07670"
     CDS             complement(<1..318)
                     /locus_tag="PLJ82_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOR41417.1"
                     /translation="MEPIVLAYIGIGLMVGLAGTGSAFGCSIAGSASVGAMKKNADGF
                     GSYLLLTALPGSQGLYGFAGYFLMSNFFGLLTTDITWLQAGAVFGSGLAVGLVGLLSA
                     IRQG"
     gene            complement(351..2183)
                     /locus_tag="PLJ82_07675"
     CDS             complement(351..2183)
                     /locus_tag="PLJ82_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOR41418.1"
                     /translation="MIVPMMKYSFLVYHADYATFLSEIKKMGVLHIQTKQNEQSAEIQ
                     DKYRSLTDLFKTIRSLEERKQADVDGKAFFDESISGEEVLEKLRAIEKEIEHKNHQLS
                     SLEKEIQQLYPWGDFSALLIKEIASIGVTIRFLICKEKKYNPEWEQVYCIEHISNHAG
                     FSHFIVCQQHDEDISELLSLVDVDEITIPTITLSEAEAKQRVVQEEINSLQQQITAIA
                     THHLMALKNLQNTWESAIADSNAYLQTEKTVDNYVMLIEGYIPEPKTEELHAYIAMHD
                     IVYTAEKPLDEEKVPVLLKNNRFTKVFEFIGSLYELPNHKELDLTPFFAPFYMLFFGF
                     ALGDAGYGLLIVIAATVLKFKKPAMKGILTLAQFLGISTVIFGALTGTLFGINLMNVD
                     IAWLQNAKQYMINTDKMFTLALALGTIQILFGMVLKVVNLSMVKGFAYAYSTIGWLIL
                     IIGLGANYFLTQLQAYTPQSSTYIQYGVLSISGALILLFNNPKRNILMNFLAGLWDVY
                     GMLTGLIGDLLSYIRLFALGVSSAILGFVFNELAMSLRPDNIILGPIVMIIILVLGHG
                     MTIFMSALGAFVHPIRLTFVEFYKNAGFVGGGKAYKPFAEKKEN"
     gene            complement(2180..2779)
                     /locus_tag="PLJ82_07680"
     CDS             complement(2180..2779)
                     /locus_tag="PLJ82_07680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOR41419.1"
                     /translation="MAIKFQYNKTSLQHLDKQLKVRKRALPTLKNKESALRSEVKRAK
                     DKAATLEEEFDKEWNKYTYMVKLWGEFDQSLIRVEDVVLSSKKIAGVLTPLLDEVKFS
                     IAPYNPFTAPMWFADGIEILRSLATIGINRAVWLRKMELLEYARKKTTQKVNLYEKVQ
                     IPGFEEAMKKIKRFLEDEENLSKSAQKIVKTRQEEEAQV"
     gene            complement(2795..>3669)
                     /locus_tag="PLJ82_07685"
     CDS             complement(2795..>3669)
                     /locus_tag="PLJ82_07685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR41420.1"
                     /translation="PDQPYNQVMASVALRAKTDKIILGGMGLTNDDYLYFKNVFENAG
                     ALEKIISFVNTTDNPPVERLLIPDMALTAAEYFAVDKNESVLVLLTDMTLFADALSIV
                     SNRMDQIPSKDSMPGSLYSDLARIYEKAVQFPSGGSITIIAVTTLSGGDITHAIPDNT
                     GYITEGQLFLRRDTEIGKVIVDPFRSLSRLKQLVIGKQTREDHPQVMNAAIRLYADAS
                     NARTKMENGFDLTDYDERTLEYAKAYSEKLLAIDVNVDTETMLSISWDLFSTHFSKAE
                     VGIKAAITEKYWKN"
BASE COUNT         1085 a          731 c          620 g         1233 t
ORIGIN      
        1 accttggcgt atagctgata aaagacccac taaacctact gccaaacctg acccaaatac
       61 agcacctgct tgcaaccatg taatgtctgt tgtaagtaat ccaaagaaat tactcatcaa
      121 gaaatatcct gcaaagccat ataatccctg actaccaggc aatgcagtta ataataaata
      181 cgaaccaaat ccgtccgcat tttttttcat tgcccccaca gaggcactac ctgctattga
      241 acagccaaac gcacttcctg tgccagctaa tccaaccatt aatccaattc cgatgtaagc
      301 taaaacaata ggttccattt tttttctttt tttaattgtt gttattctta ttaattttct
      361 tttttctcgg caaacggttt gtaggctttt cctccaccta caaatcctgc atttttataa
      421 aattctacaa atgttaatcg aataggatga acaaaagcac ccaatgcaga cataaaaatt
      481 gtcattccgt gacctaatac caagataatt atcattacta tgggacctaa tatgatatta
      541 tctggacgta aactcatagc caattcatta aacacaaatc ctaaaatagc actagatacc
      601 cccaatgcaa atagtcgtat ataggataat aagtcgccaa tcaagcctgt aagcatgcca
      661 tatacatccc acaacccagc taaaaagttc attaaaatat tccgtttagg attgttgaat
      721 aataaaatca aggctccaga aatgcttaat actccatatt gtatgtatgt tgacgattgg
      781 ggtgtgtaag cctgtaattg ggttaaaaaa taatttgcac ccaaaccaat aattagtatc
      841 aaccatccaa tagtagagta ggcataagca aaacctttta ccatcgacaa atttaccact
      901 tttagcacca tgccaaagag aatttgaata gttcctaaag ccaatgctaa agtgaacatt
      961 ttgtccgtat ttatcatgta ttgcttcgca ttttgtaacc atgcaatatc cacattcatt
     1021 agattgatac caaacaatgt tccagtaagt gctccgaaaa taaccgtaga aatgcctaag
     1081 aactgtgcaa gagtaagtat gcctttcata gcaggtttct taaactttag tactgtagcg
     1141 gctattacta tcaataatcc ataacccgca tcgcccaaag caaaaccaaa aaacaacata
     1201 taaaatggcg caaagaaagg cgttaaatct aattctttat gattcggtaa ttcgtacaaa
     1261 ctgccaataa actcaaaaac ttttgtaaaa cgattatttt taagtagcac aggaactttt
     1321 tcttcgtcaa gcggtttttc tgctgtgtac actatgtcgt gcatggcaat gtatgcgtgt
     1381 agctcttctg ttttaggttc aggaatatac ccttctatca acatgacata attatcaaca
     1441 gttttttccg tttgcaaata agcatttgag tcagcaatag ccgattccca tgtattttgt
     1501 agatttttta aagccataag atggtgtgta gcaatagccg tgatttgttg ctgaagactg
     1561 tttatttctt cttgaaccac acgctgttta gcttctgcct ctgacaaggt tatggtgggt
     1621 atagtaattt catctacatc taccaaggat agtaattcgc tgatgtcttc gtcgtgttgt
     1681 tggcaaacga taaagtgaga aaagcccgca tgattggata tgtgttcgat gcaatacact
     1741 tgttcccatt caggattgta ttttttttct ttacaaatta aaaagcgaat tgtaacacca
     1801 atggaagcaa tttcttttat caatagtgca gaaaaatctc cccatggata cagttgttgt
     1861 atttctttct ccaaagaact aagttgatga tttttgtgtt caatttcttt ctcaatagct
     1921 ctaagttttt ccagcacttc ttcgcccgaa attgactcat caaagaatgc tttaccatca
     1981 acatcggctt gttttcgttc ttccaaactc cgtatagttt tgaataaatc agtaagagaa
     2041 cggtatttat cttgtatttc agccgattgt tcgttttgtt tagtttgtat atgcagtaca
     2101 cccattttct ttatttcaga aagaaatgta gcataatcgg catgatacac caaaaacgaa
     2161 tatttcatca taggtactat catacttgtg cctcctcttc ttgtcgtgtt ttcacaattt
     2221 tttgagccga tttcgataga ttttcttcgt cttctaaaaa tcgtttaatt tttttcatgg
     2281 cttcttcaaa accaggtatt tgcacttttt cgtataagtt tactttttgc gtagtttttt
     2341 tgcgtgcata ttccaataac tccattttac gtagccatac agcacggtta attccaatag
     2401 ttgctagtga tcgaagtatc tcgatgccat cagcaaacca cattggggcg gtaaaaggat
     2461 tgtatggagc aatagaaaat ttcacttcat ctaacaaggg cgtgagcaca cctgcaattt
     2521 tttttgaaga taatactaca tcctctacgc gtattaacga ttggtcgaat tctccccata
     2581 gctttaccat ataggtgtat ttattccatt ctttgtcaaa ctcttcctct aacgttgctg
     2641 ctttatcttt ggcgcgtttt acttcgctac gcaaggctga ttctttgttt ttcagcgttg
     2701 gtagtgctct tttccttacc ttgagttgtt tgtcgaggtg ctgtaaagag gttttattgt
     2761 attgaaattt tatcgccata aactactagg gtgcttaatt tttccaatat ttttcagtaa
     2821 ttgctgcttt aatacctact tctgctttcg agaaatgtgt actaaataaa tcccaagaga
     2881 tagaaagcat ggtttcggta tctacattta catcaattgc caatagtttt tctgaatagg
     2941 ctttggcata ttctagcgta cgttcgtcat agtctgttag gtcaaatccg ttttccattt
     3001 tggtgcgtgc atttgatgca tctgcgtaca aacgaatggc tgcattcatc acttgcggat
     3061 ggtcttcgcg tgtttgtttg ccaataacca attgttttaa acgtgataat gagcggaatg
     3121 ggtcaacaat tacttttcca atttcggtat cgcgacgcaa gaataattgt ccttcggtaa
     3181 tataccctgt gttatctgga atagcatgtg taatatcgcc accagatagg gtggtaactg
     3241 ctataatggt aatggaacct cccgatggaa attgtactgc tttttcgtat atacgagcta
     3301 agtctgaata caacgaaccg ggcatactgt ctttagatgg aatttgatcc attctatttg
     3361 acacaatact taaggcatct gcaaacaagg tcatgtcggt aagtaacacc aatacgcttt
     3421 cgtttttatc aactgcaaaa tactcggctg cagtaagagc catatctggt attaataagc
     3481 gttctacagg agggttgtcg gtagtattta caaagctgat gattttttct aatgctcctg
     3541 cattttcaaa tacgttttta aaatacaaat agtcatcgtt agtaagtccc atacctccta
     3601 aaataatctt atcggttttg gcacgcaacg ctacgctagc cattacttga ttgtaaggtt
     3661 ggtcagggt
//
LOCUS       DAOSRP010000135         3634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_22985_length_3634_cov_4.186085, whole genome shotgun sequence.
ACCESSION   DAOSRP010000135 DAOSRP010000000
VERSION     DAOSRP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22985_length_3634_cov_4.186085"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLJ82_07690"
     CDS             <1..1130
                     /locus_tag="PLJ82_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR41421.1"
                     /translation="TELAVTMENAIGGIILTASHNPTQWNALKLLNEKGEFLDDKAGK
                     AILEIANDESFQFADVLELGKLTENNTFNQKHIDSVLSLKLVDVDAIRNAKFRVAIDC
                     VNSVGGIIIPQLLSALGVTEIVPLYCEPNGKFPHNPEPLPEHLTDIASILTNGKADVG
                     FVVDPDVDRLAIISENGEMFGEEYTLVAVADYVLSHTPGNTVSNLSSTRALRDVTQQY
                     GATYTAAAVGEVNVVSKMKDTHAIIGGEGNGGVIYPESHYGRDALVGIALFLSHLAHK
                     KMKVSALKATYPPYFISKQKVQLTPSIDVDAILNKVKVAFSTEKITDIDGVKIDFPDK
                     WVHLRKSNTEPIIRIYAEAATMEQADKLGAEIIAIIQQFAQ"
     gene            complement(1195..1683)
                     /locus_tag="PLJ82_07695"
     CDS             complement(1195..1683)
                     /locus_tag="PLJ82_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41422.1"
                     /translation="MKKLLLFILSSFMLVSCSLQSEADMSPQISFYDITINGESTTGQ
                     SGILVEDTLRMTVALQGYYHELTELVIVSEDDFVEFSFDESFQSSDWFASEITTSGTR
                     KFIFKSGIKTCVIPIQVVGIQKKQQAVELNFSLQSQSSAKGDYNPRKIDFAFTVSEQA
                     EE"
     gene            1816..2547
                     /locus_tag="PLJ82_07700"
     CDS             1816..2547
                     /locus_tag="PLJ82_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOR41423.1"
                     /translation="MIQEAITNYKLQHTITTLSPLKAILFDMDGVIFDSMPYHALSWV
                     NAFKQVGITFSEYEVYLNEGRTGASTIHNEFLSQKHRQASDAEIKELYALKSQHFEAC
                     GKPIAMNYATETLNRVKHLGLDIFLVTGSGQVSLLNTLNTYFPAVFSKQNMVTAYDVT
                     HGKPHPEPYLIALEKGNLQAQQAFVVENAPLGIQAAVAANIFTVAVNTGILEDKVLLE
                     AGANIVVPSLADFYHLLPSLVENWK"
     gene            complement(2549..3169)
                     /locus_tag="PLJ82_07705"
     CDS             complement(2549..3169)
                     /locus_tag="PLJ82_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOR41424.1"
                     /translation="MKKFLFILSLLLFTSALYAQNIKLPTPNKSGGKPLMTVLNERKS
                     SRDFSEKILPNQQLSNLLWAAWGYNRADKRTAPSSQNKQEMDIYVALASGCYLYDARK
                     NELVLVVKQDLREKTGKQDFVKNAPVNIIFVADKRKMAQQDEQSMLQTAYINTGFISQ
                     NIYLYCASEGLATVIRAWVDKAALALAMKLHDQQEIIVCQTVGYPK"
     gene            complement(3212..3460)
                     /locus_tag="PLJ82_07710"
     CDS             complement(3212..3460)
                     /locus_tag="PLJ82_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41425.1"
                     /translation="MLSDKDLFELVGIRIRNIRKSQGMSQQILAYEVDMEKTNISRIE
                     AGRTNVTVKNLYKIAQALGVTMKDIVDIEREHKETKDM"
BASE COUNT         1223 a          656 c          593 g         1162 t
ORIGIN      
        1 ctacagaatt agcagtaacc atggaaaatg ctattggcgg tattatactt actgctagcc
       61 ataatccaac ccaatggaat gcactaaaac tgctaaacga aaagggagaa tttttagacg
      121 ataaagcagg taaagcaatt ttagaaattg ctaacgatga aagctttcaa ttcgcagatg
      181 tattggagtt aggtaaatta actgaaaata atacattcaa tcaaaaacat atcgacagcg
      241 ttctttcact caaactagtg gatgtagatg ctatacgcaa tgcaaaattc cgtgttgcca
      301 ttgactgtgt caattcagtt ggaggtatta taattcctca acttttatct gcattagggg
      361 taacagaaat tgtaccttta tattgcgaac ctaatggtaa gtttccacac aaccctgaac
      421 ccttacccga acacttaact gacatagctt ctattctcac aaacggaaaa gcagatgtag
      481 gttttgtagt agacccagac gttgatcgtt tagcaattat ctctgaaaat ggtgaaatgt
      541 ttggagaaga atataccttg gtggctgtag ctgattatgt attgagccac acacctggta
      601 atacagtatc caatttaagt tcaactagag ccctacgtga tgttacccaa caatatggag
      661 ctacctatac cgctgcagct gttggtgaag taaatgtggt aagcaaaatg aaagacacac
      721 atgccattat tggaggcgaa ggtaatggtg gagttatcta ccctgagagc cactatggaa
      781 gagatgcttt agtgggaata gctctgtttc ttagtcattt agctcataaa aaaatgaaag
      841 tatctgcact aaaagcaact taccctcctt attttatttc caaacaaaaa gttcaactga
      901 caccatcaat tgatgttgat gctattttaa acaaagtgaa agtagcattt tctacagaaa
      961 aaattactga tattgatgga gtaaaaattg attttccaga taaatgggtt catttgcgta
     1021 aatcaaatac agaacccatc attcgcattt atgccgaagc agccactatg gaacaggctg
     1081 acaaattggg agctgaaatt atagccatta tacaacaatt tgcccaataa aatacctaaa
     1141 aataaattta gtcttataca atcaaatcta cattgaggct atataagact aaatttattc
     1201 ctctgcttgt tcagacacag taaaagcaaa atcaattttt cgtggattat aatctccctt
     1261 tgccgaagat tgtgattgta atgagaaatt taattccaca gcttgttgtt tcttttgtat
     1321 cccgactacc tgaatgggta tcacgcatgt tttgatgcca gatttaaaaa tgaactttct
     1381 tgttcctgag gtagttattt cagaagcaaa ccaatcactt gattgaaatg attcgtcaaa
     1441 cgaaaattct acaaaatcat cttctgacac aattactaac tccgtaagtt cgtgataata
     1501 accttgcaat gcaacagtca ttcgcaaagt atcttctact aaaataccgc tctgtccagt
     1561 agtcgattct ccattgatag ttatatcata aaaactaatt tgtggactca tgtctgcttc
     1621 tgactgtaag ctacacgaca ccagcataaa actagacaga ataaataaaa gtaatttttt
     1681 catgtttatt taactttagg tatgttagaa tgcctgcaaa tatacagaag gcgaataaca
     1741 tatacgtact tttttattta aatagtgtaa aaaggtaggt aaaattgtgt aattttgtgt
     1801 gcaaataaaa aatacatgat acaagaagct attaccaact ataaactaca acacactatt
     1861 actacgctta gtccactcaa agctattttg tttgatatgg acggagtaat ttttgattca
     1921 atgccctatc atgccctttc gtgggtaaat gcatttaaac aagttggtat cactttttct
     1981 gaatacgaag tatatctcaa cgaggggcga acaggggctt ctaccattca taatgaattt
     2041 ctttctcaaa aacacagaca agcttctgat gctgaaataa aagaattata tgccttgaaa
     2101 agtcaacatt ttgaagcatg tggtaagcca attgcgatga actatgctac cgaaactctc
     2161 aatcgagtta aacatcttgg attagatata tttttggtta caggttctgg tcaagtaagt
     2221 ttattaaaca ccctaaatac ctatttccca gcagtatttt caaagcaaaa tatggttaca
     2281 gcgtacgatg taacacatgg caaaccacat cctgaaccct atctcatagc tttggaaaaa
     2341 ggaaacttgc aagctcaaca agcttttgta gtagaaaatg ccccattagg cattcaagct
     2401 gctgtggcgg ctaacatatt tacagttgcc gttaatactg gaattttgga agataaagtg
     2461 ttgttagaag caggtgctaa tattgtcgtt cctagtttag cagattttta tcacttacta
     2521 ccctcgcttg tggaaaactg gaaataaatc actttggata tcctactgtt tgacaaacaa
     2581 tgatttcttg ctgatcgtgc aatttcattg ctaaagccaa tgctgcttta tcgacccatg
     2641 ccctgatgac agttgctaat ccttcggagg cacaatataa gtaaatattt tggctaataa
     2701 aaccagtatt aatgtatgct gtttgcaaca tactttgctc atcttgttga gccattttcc
     2761 ttttatcggc tacaaaaatg atatttacag gagcattttt cacaaaatct tgtttgcctg
     2821 ttttttcgcg taaatcttgt ttaacaacta gtactaattc gtttttacgt gcatcataca
     2881 aataacagcc acttgctaaa gccacgtaaa tatccatttc ttgcttgttt tgagaagaag
     2941 gtgctgtacg tttatctgcc ctattataac cccaagccgc ccataataaa ttggataact
     3001 gctgattagg caaaattttt tctgaaaaat cacggctact tttacgttcg tttaaaactg
     3061 tcattaaggg cttaccacca cttttatttg gagttggcaa cttgatattt tgtgcatata
     3121 aggcactggt aaataaaagt aatgacaata taaacagaaa ttttttcata caactttgta
     3181 ttttataaaa acacctacaa atataagaaa actacatatc ttttgtctct ttatgctctc
     3241 tttctatatc cacaatgtcc ttcatggtta cacctaaggc ttgagcaatt ttatataaat
     3301 tcttgacagt aacatttgtt ctccccgctt caatacgaga gatattcgtt ttttccatat
     3361 ctacctcata cgccaagatt tgctgcgaca taccctgtga ttttcgaata tttcttatcc
     3421 gaataccaac cagttcaaac aaatctttat ctgacagcat aaaatatagt tatcatattt
     3481 ggttacaaat taaatttaat ttatctttgc aaaagttatc atatatgata ttttttgaaa
     3541 taattttaat ataatcaaaa gtatatttaa aataaataaa aggagaatgc aaaaatgaag
     3601 ggtcaatata gaaataaaag gtaattacac aaaa
//
LOCUS       DAOSRP010000136         3606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_23206_length_3606_cov_3.332019, whole genome shotgun sequence.
ACCESSION   DAOSRP010000136 DAOSRP010000000
VERSION     DAOSRP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23206_length_3606_cov_3.332019"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3606
                     /locus_tag="PLJ82_07715"
     CDS             <1..>3606
                     /locus_tag="PLJ82_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOR41426.1"
                     /translation="LGTARLNNVTPGSYYIQYKIADINGCQAEKTKMLTVIDEKQGPL
                     DFGVFDIRPNPMCINSTEAQIIGQRSDITYVGLSVPAMQISQNQGVLYLDPSRGRIGN
                     HSVIAQRIDVNGCVAKQRVPFQIVAPTRVFPFNIAKTYCTYDEDVQLVVNSASPTTGT
                     ISIDNITDPLHPVNVLSTLDPASGNVYFQPGWGAGKYEIRYEYSDGNCDNIQRDTVDV
                     YAPSVIDMNLKSDYCIKDVITLAATPLGGIYSSDAVANALVNNRFYTERAGLGLHKIR
                     YIQTNEYGCISKDSTTIQVRGASVLTIADLKDQYCEPKGTVNIYGFPNNPADGSVWFT
                     GPTFLTSLPNGYASLDLSQATYNANYNVTYHYQENYVNAQGTPESCTTTVTKGFRVLN
                     EAADFWGYNHMDVICGTQDSVVITGNKAPLGRFVFTGPAANFKDRGNGTAVIYPPTLP
                     EGYYQVTYHYEHKIAGVTVCATEQAKTFEIAQIAPIEDISVFCSSGDNAIRIPNTEVG
                     VEYELQVNGVSVEKKMGLGAGLYLEFSPITDDVAQIKVFARLGQCVIKASQEFTVRKL
                     RVLTNKTDITCHGANDGKMLSTISGGGYPYSYSFEKIIGTPVMPTLFDSVAVNLTPGT
                     YRFTVTDTIGCELQRQIAIIEPQLLSAIVESESVKCPTTRLGNASVIPSGGRPPYKYK
                     WYNSAGLLIDSVASISNLTSDTHRVEVFDRSGCKVERFATITTPTPIKIDVTKLTHVV
                     ITGQNTGEIELNVTGGTLPYTNEWAGVGINPANVTNLNQYNLIAGNYQFTVTDNFDCK
                     KDTVITITQPETIKVQSLIQHVSCFGSNDALIQLTITGGVRPFTYSWTGPNGFTSSDE
                     DIQNLAPGEYKFRLEDSNGNTFGYQSYFVKEPDLLEMHTLNTSLLAVSCHGDKTANID
                     VEVLGGTTPYNYTWTGPDVPSTITNPTSLVNLGAGTYQVQVRDKNNCITNLSAIIISP
                     AKLNLAHTLVDNKCHNESAASITLTPSGGTPHVAPAEPYLFFWTGDGANPYDQNQTNL
                     TAGETYTVTVQDANYCEVTKEFILQNPTELLIDVVITPISCAGRHDGSITVNVQGGVP
                     TYSVEIRKDDNTVIINGSSANTLYSGTYIITVIDALGCEKTTSATLAPKDPLTVSITK
                     EDVLCHGANSGIARATPSGGTEPYSYNWTKQPSNDFVDDLRAISYLMPGNYKVDVS"
BASE COUNT         1188 a          638 c          723 g         1057 t
ORIGIN      
        1 cttaggaact gctcgtttaa ataatgtaac tccaggttcg tattatatac aatataaaat
       61 agcagatatt aatggttgtc aagcagaaaa aacgaagatg ctgactgtta ttgatgaaaa
      121 acaaggacct ctagattttg gtgtttttga tattagaccc aatcctatgt gtatcaattc
      181 tactgaagct caaattattg gtcagcgatc agatatcacg tatgtaggct tatccgtacc
      241 tgcaatgcaa atttctcaga atcaaggcgt tttatatctt gatccgtcac gtggcaggat
      301 tgggaatcat agcgttattg cacaacggat agatgtgaat ggttgtgttg ccaaacagcg
      361 agttccattt cagatagtag cacctacgag ggtatttcct ttcaatattg ctaaaacata
      421 ttgtacctac gacgaagatg tgcaattagt agtaaattct gcgtcaccta ctacaggaac
      481 tatttctatc gataatataa cagacccatt acatcctgtg aatgtactct ctacgcttga
      541 tccggctagt ggaaatgtat atttccaacc agggtggggt gcaggtaagt acgaaatccg
      601 ttacgaatat tctgatggaa attgtgataa tatacaaaga gatactgtag atgtatatgc
      661 tccttcggta attgatatga atttgaaatc ggattactgt atcaaagatg taattacttt
      721 agcagcaact ccattaggtg gtatttattc atctgatgcg gttgcgaatg ctcttgttaa
      781 caatcgtttt tatacagaaa gagcaggatt gggtttacat aaaattaggt atattcaaac
      841 caatgagtat ggatgtattt ctaaagatag tactacaata caagttagag gtgctagtgt
      901 tttgactatt gccgatttaa aagaccaata ttgtgaaccc aaaggaactg tcaatattta
      961 tggtttccca aataacccag ctgatggtag cgtttggttt actggaccaa catttctaac
     1021 aagcttgcca aacggatatg caagtttaga tttatcacaa gctacctata atgcgaatta
     1081 caacgttaca taccactatc aagagaatta tgtaaatgca caaggtacgc cagaatcatg
     1141 tacaactact gtaaccaaag gctttagggt gttaaatgaa gcagccgatt tttggggata
     1201 caaccacatg gatgtaatat gtggtacaca agattctgtg gtaataacag gtaataaagc
     1261 cccacttggc aggtttgtat ttactggacc agcagccaat tttaaagata gaggaaacgg
     1321 aacagccgtg atttaccctc caaccttgcc tgaagggtat tatcaggtaa cataccacta
     1381 cgaacacaaa attgcaggtg ttacagtgtg tgctacggaa caagccaaaa catttgaaat
     1441 agctcaaata gcacctatcg aagatatttc tgtgttttgt agtagcggag ataatgccat
     1501 tcgtattccc aatacagaag taggagtaga atatgagttg caagtaaatg gggtatctgt
     1561 tgaaaagaaa atgggattag gagcaggtct gtatttggag ttttctccta ttactgatga
     1621 tgtagcacaa attaaagtat ttgctcgtct tggacaatgt gtaatcaagg cttctcaaga
     1681 gtttacagta cgaaaattaa gagttctcac gaacaaaaca gatatcactt gccatggagc
     1741 aaatgatggt aaaatgctct caacaatatc aggtggtggc tatccttatt catattcatt
     1801 tgaaaagata ataggcactc cagtaatgcc gacattattt gattctgttg cagtaaatct
     1861 tacacccgga acctaccgtt ttactgttac tgataccatt ggctgtgaat tgcaacgtca
     1921 gatcgcgatt attgaacctc aattactttc tgctattgtg gaatccgagt ccgtaaaatg
     1981 ccctactaca cgtttaggta atgcaagtgt tattccttct ggtggcagac caccctataa
     2041 gtataaatgg tataattcgg caggtctgct tatagattca gtagcatcaa ttagtaattt
     2101 aacctcagac acacatcgtg tagaagtgtt tgatagaagt ggatgcaagg tagaaaggtt
     2161 tgcaactatt acgactccca ctccgattaa aattgatgtt acgaaactta cccatgtggt
     2221 aattacagga caaaatacag gagaaataga attgaatgta actggcggaa cacttcccta
     2281 tacaaatgaa tgggcaggtg ttggtataaa tcctgcaaat gttacaaatc ttaaccaata
     2341 taatttaata gctggcaatt atcaatttac tgtaacagat aattttgatt gtaaaaaaga
     2401 tacagtcatt accattacgc aaccagaaac gataaaggta caatcactta ttcaacacgt
     2461 atcatgcttt ggtagtaatg atgctcttat tcaattaact attacgggag gtgtacgtcc
     2521 atttacctat agttggactg gacctaatgg gtttacttca tcagacgaag atattcaaaa
     2581 tttggctcca ggagaatata aattcagatt agaagatagt aatggcaata catttggtta
     2641 tcaaagttat tttgttaaag aaccagattt actcgaaatg cacacgctaa atacatcatt
     2701 actagcagta tcgtgtcatg gagataaaac ggcaaatatt gatgttgaag ttcttggggg
     2761 tactacgcca tataattaca catggacagg acccgatgtg ccaagtacaa taacgaaccc
     2821 aactagttta gtgaatttag gagcaggaac gtatcaagtg caggtgcgtg ataagaataa
     2881 ttgtattaca aatttatcag ccattattat atcaccagcc aagttgaatc ttgctcatac
     2941 tttagtggac aacaaatgtc ataatgaatc ggctgcttct attacgttaa caccaagcgg
     3001 tggaactcca catgtagcac cagcagagcc atatctgttt ttctggacag gagatggtgc
     3061 aaatccgtat gaccaaaatc aaactaattt aactgcaggg gaaacctata cagttactgt
     3121 gcaagacgca aattactgtg aagtaacaaa agagtttatt ttgcagaatc caacagaatt
     3181 gctaatagat gttgttatca ctcctataag ctgtgcgggc agacacgatg gctctattac
     3241 agtaaatgta caagggggcg tgccaacata ctctgttgaa attcgtaaag atgataatac
     3301 ggtgataatt aatggtagta gtgctaacac cttgtattct ggtacctata tcattacagt
     3361 gattgacgca ttaggatgtg aaaaaaccac atcagcaacc ttagctccta aagatccatt
     3421 gactgtatcc ataaccaaag aagatgtttt gtgtcatgga gctaattcag gaatagcaag
     3481 agcaacaccg tcgggaggta cagagccata tagctataat tggaccaaac aaccttcaaa
     3541 cgattttgtg gacgacctac gagctatcag ttacctaatg cctggtaatt ataaggttga
     3601 tgtttc
//
LOCUS       DAOSRP010000137         3574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_23489_length_3574_cov_4.649332, whole genome shotgun sequence.
ACCESSION   DAOSRP010000137 DAOSRP010000000
VERSION     DAOSRP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23489_length_3574_cov_4.649332"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLJ82_07720"
     CDS             <1..290
                     /locus_tag="PLJ82_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41427.1"
                     /translation="RTHDPEITNHVLWPTELCWQVGKLSTSHRSTNYPCCGQTLGDSK
                     GADRIGLTHRKSNAFCLIDQESALKNDTKNHFFVNQYRYSFFLVLQLIIIC"
     gene            303..857
                     /locus_tag="PLJ82_07725"
     CDS             303..857
                     /locus_tag="PLJ82_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOR41428.1"
                     /translation="MWIFILFMMKDSSSSSTLVQSAMFYGMYLGAFLLFRFGLNVLAT
                     DTVILSFVVFFLTLFIPILAYILLVRFKKSLPLMEINFRQAYMFTFFLFLFGSLILAI
                     VQYFYYQYINTDFLYQQYQVVLQNSTLLLQEFPQMKPFIQELKQSGVPSAIAVASQNI
                     WIYSFTGIIIGIVLGLIVKTKQKT"
     gene            862..1827
                     /locus_tag="PLJ82_07730"
     CDS             862..1827
                     /locus_tag="PLJ82_07730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR41429.1"
                     /translation="MDISVVVPLYNEEESISELFSWIKRVMDDHGFSYEVIFISDGST
                     DKSWQVIEQLGKENVAVRGIKFRRNYGKSPALYCGFKESQGDVVITMDADLQDSPEEI
                     PALYQMIMKDRYDLVSGWKKKRFDPVFTKNLPSKLFNATARKISGIKLHDFNCGLKAY
                     KNEVVKNIEVYGEMHRYIPFLAKNAGYTRIGEKVVSHQARKYGNSKFGFARFINGYLD
                     LLSLWFISRFGVKPMHFFGVAGSIMFLLGLLSVVAVGANKLFAIIQGVRAPLVTDSPY
                     FYLAMLAMVLGTQFFVTGFLGELISRNAAERNNYHIERTHNIEHI"
     gene            1839..2366
                     /locus_tag="PLJ82_07735"
     CDS             1839..2366
                     /locus_tag="PLJ82_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41430.1"
                     /translation="MDILWQILSYTIPSLFVLIVAYFTLKMVFQNEEKRRRYELFKAN
                     LSLITPVRLTAYERLVLFVERISPDSLMVRVQDSSLTSAQFHSFLLATIRAEYEHNLS
                     QQVYVSPEAWTFVKNAKESMIQLINTSASNVPPQVPSFELAKVILDTYQNTSNETPTM
                     FAINFLKSEIKQYFG"
     gene            2386..2892
                     /locus_tag="PLJ82_07740"
     CDS             2386..2892
                     /locus_tag="PLJ82_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR41431.1"
                     /translation="MKLLERIQTRYSVRSFLPKQVEKEKIDYLLECARWAPSAVNAQP
                     WHIIVVQQEKNKRALHQCYQREWFKQAPMYMLVCGNHQESWKRKYDNNDHCSIDIAIA
                     VEHIALAVQEVGLGTCWVCNFDAVLCAELFELPNHIEPIVILPIGYPNDSLNEKKRKL
                     IQDIVEWK"
     gene            2883..3362
                     /locus_tag="PLJ82_07745"
     CDS             2883..3362
                     /locus_tag="PLJ82_07745"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOR41432.1"
                     /translation="MEITSLVKNIYNRLIVFICLACIFSACVEKECITYKHVLVGIEL
                     IQDTSNTVYTADSITVKGVGSDEIIYDNAKNISSIYLPLKSVSNTTSFDIVLNETPFV
                     LSITHQNIPQLLSIECGTVMFHKIEDATIDGEIQAVIQVENTHVQNRENEKHIYIRL"
BASE COUNT         1171 a          520 c          651 g         1232 t
ORIGIN      
        1 ttcgaaccca cgaccccgag attacaaatc acgtgctctg gccaactgag ctatgctggc
       61 aagtgggtaa gctatctaca tcgcatcgct caactaacta cccttgctgc ggtcaaaccc
      121 tgggggattc gaaaggagca gatcgtatag gacttaccca ccgcaaaagt aatgcttttt
      181 gcttaattga ccaagaaagt gctttaaaaa atgacacaaa aaaccatttt tttgtaaatc
      241 aatatcgtta ttcatttttt ttagtattac aacttataat aatatgctaa atagttgtac
      301 ctttgtggat atttattttg tttatgatga aagattcttc ttcttcctct acattggttc
      361 agtctgccat gttttatggt atgtatctag gtgcattttt attgtttaga tttggtttga
      421 atgtgcttgc tactgataca gttatacttt cttttgttgt tttttttctc actcttttta
      481 tcccaatatt ggcttacata ttactagtaa gatttaaaaa aagtttacct ttaatggaga
      541 taaattttag gcaagcttat atgtttacat tttttctttt tctatttggt agtttaattt
      601 tagctattgt gcaatatttt tattaccaat atataaatac agatttttta tatcaacaat
      661 accaagtagt attgcaaaat tccacattgc ttttgcaaga atttccacag atgaaacctt
      721 ttatacaaga attaaaacaa tcgggggtac catcagctat tgctgttgct agtcaaaata
      781 tctggatata ttcgtttact ggcattatca tagggattgt acttggattg atagtaaaaa
      841 caaaacaaaa aacatagagt aatggatata tcagttgtag tgcctttata caatgaagaa
      901 gagtctattt cagaattatt ttcatggatt aaacgtgtga tggatgatca tggctttagt
      961 tatgaagtaa tatttatttc tgacggaagt accgataaat catggcaagt aattgaacaa
     1021 ttgggaaaag agaatgtagc agtacgtggt ataaaatttc gacgaaatta tggtaaatca
     1081 ccagccttgt attgtggatt taaagaatct caaggagatg ttgttattac tatggatgct
     1141 gatttacaag atagtcctga agaaataccc gctttgtatc agatgattat gaaagataga
     1201 tatgacttag tttcgggttg gaaaaagaaa cgttttgatc ctgtctttac aaaaaatcta
     1261 cccagtaaat tatttaatgc tactgcacgt aaaatttcgg gtattaagtt gcatgacttc
     1321 aattgtggct tgaaagcata taaaaatgaa gtagtgaaaa atattgaggt gtatggagaa
     1381 atgcatcgtt atatcccatt tttagcaaaa aatgctggct atacacgtat tggagaaaaa
     1441 gttgtaagtc atcaagcacg taaatatgga aatagcaaat ttgggtttgc tcgatttata
     1501 aacggatatt tagatttact atcattgtgg tttatttcgc gatttggtgt aaaacctatg
     1561 cacttttttg gtgtagcagg tagtattatg tttttacttg ggttgttatc tgttgtagcg
     1621 gtaggtgcaa ataagttgtt tgctattata cagggtgtac gagctccttt ggttaccgat
     1681 agtccatatt tttatcttgc aatgcttgct atggtacttg gtacacaatt tttcgtaaca
     1741 gggtttttag gcgaattaat ttcacgtaat gctgctgagc gtaataatta tcatatagaa
     1801 agaacacata atattgaaca tatttaattt aagaacaaat ggatatactt tggcaaatac
     1861 tctcatacac tattccgtct ttatttgttt tgattgtggc atattttact ctaaagatgg
     1921 tgtttcaaaa tgaagaaaaa agacgtagat acgaactttt taaagcaaac ttatctttaa
     1981 ttacaccagt tagattaaca gcatacgaaa gattagtgct gtttgttgag aggattagtc
     2041 ctgattcatt aatggtaaga gtacaagata gttcgcttac ctcagcacaa tttcattcat
     2101 ttttgttagc aactatacgt gctgaatacg aacacaattt atctcaacaa gtatatgtaa
     2161 gccccgaagc atggactttt gtgaagaatg caaaagaaag tatgattcag cttatcaata
     2221 catcggcatc taacgttcca ccccaagtgc cttcgtttga attggcaaag gttattttag
     2281 atacatatca aaacacatcg aatgaaactc ccacaatgtt tgcaataaat ttcttaaaat
     2341 cagaaattaa acaatatttt ggttgataat gagataaaaa taactatgaa attgttagaa
     2401 cgtatacaaa caagatattc tgtaaggtct ttcttgccaa aacaggtaga aaaagaaaaa
     2461 attgattatt tgttagaatg tgctagatgg gctccttcgg cggttaacgc tcaaccatgg
     2521 catataatag ttgtgcaaca agaaaagaat aaacgagccc tccaccaatg ttatcaaaga
     2581 gaatggttca aacaagctcc tatgtatatg cttgtgtgtg gtaatcatca agaatcgtgg
     2641 aaacgaaaat atgataataa cgaccattgt tccatagata ttgctattgc agttgaacat
     2701 attgctttag cagttcaaga agttggtttg gggacatgtt gggtgtgtaa ttttgatgct
     2761 gtattgtgtg ccgagttatt tgaattacct aatcacatag agcctattgt cattttgccg
     2821 atagggtatc ctaacgattc tctgaatgaa aagaaacgaa aattgataca agatattgtt
     2881 gaatggaaat aactagtttg gtgaaaaaca tatataatag attgattgta tttatttgtt
     2941 tggcatgtat tttttctgca tgtgtagaaa aggaatgcat tacttataaa catgttttag
     3001 tgggcattga attgatacaa gatacatcca atactgtgta tactgcggat agtattacgg
     3061 taaaaggagt aggatcggat gaaattattt acgataatgc aaaaaacatt tcatccattt
     3121 atttaccatt aaaatcagtt agtaacacca ctagttttga tattgtattg aatgaaactc
     3181 cctttgtttt atctattaca catcagaata tccctcagct tttgagcatc gaatgtggaa
     3241 cagtaatgtt tcataaaata gaggatgcta ctattgatgg agaaatacaa gcagttattc
     3301 aagttgagaa tacacatgta caaaatcgag agaatgaaaa acatatttat atacgtttgt
     3361 agttgttttc tgtgcatagt ttctgtactc aatacgaatg cacaggaact ttctaatcca
     3421 gagaaacttt ccatgccatt gtatcaaggc tcttttattg gattaaatct tgcaccgtct
     3481 gtagagagtt tctttgtacc atcctatttt tcaacagaag tttcgataga tgttagttta
     3541 aaacaaaaat attttcctat agttgaggca ggta
//
LOCUS       DAOSRP010000138         3542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_23755_length_3542_cov_3.975624, whole genome shotgun sequence.
ACCESSION   DAOSRP010000138 DAOSRP010000000
VERSION     DAOSRP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23755_length_3542_cov_3.975624"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLJ82_07750"
     CDS             complement(<1..312)
                     /locus_tag="PLJ82_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41433.1"
                     /translation="MNKPIISIISSIAGICTLLFIVLHLAVNLLLVLDAIANTQGYYY
                     NSMHYFMQHNSVAYTLEKTFALGFVLHILLANILAFNKIRNKQGSKDMIIMGFLLLIF
                     TV"
     gene            complement(380..2593)
                     /locus_tag="PLJ82_07755"
     CDS             complement(380..2593)
                     /locus_tag="PLJ82_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOR41434.1"
                     /translation="MKIISTACLLMLVSFFVLADDKSTVELNEFVVNSLPKESAFTTQ
                     LATTNYTIGMQQVEKLGVQSIKNLSAHIPNYYLPDYGSKITSSMYIRGIGSRMNEPAI
                     GMYVDNVPYIHRSAFDIMLHDIQQFQFLQGPQGTLYGRNTIGGLMNIYTISPLVYQGT
                     RLNVSYAKYNDANVSVSHYQKLNDSLGVSISGYVQHQDGHFTNMGRMEKNKSDEFGIR
                     GKLEWKMKNNWQSMFQISYDYTDQIAYPYAKFDTISKQIYPINYNEQGAYRRKMLSSG
                     FTLQKISDNYAFTSATGFQNITDRMDIDQDFTSDSIMVLQQNQHLKSITQEFVFRSNN
                     SNNWQWVNGLFLFGASNTIDSPMTFQKGGIKMIQNFMDMAKMFNPNMPTITILNEEML
                     VAGLFSKPSIGAALYHQSSYSFTNKFSVTAGIRLDYEKAFIDYDSEATLQTSIKLPSP
                     FVPAFRVDSTYAVKGFKSKDFIEVLPKVALHYKIDNQHIVYTSLTKGYKAGGYNYSMF
                     SDILQAQMQGNKDVTIDDKIYYKPEESWNVEVGSNGTFGNNRFNYQTAIFYIHHYNQQ
                     LSTTTSNGSRMISNAEVSQSIGGELSVKAYLSKQIAGYIHYGHTNASFIKYNANNINY
                     SKKSIPFAPKNTLSLGIETKVPITEEWLDEIVLSAHYTGIGPLYWNAQNTLSQEYYGI
                     ANGDITFSKNNTHLKFWVKNALNQSYNVFYFESLGNSFVQKGIPISFGIHVKHIL"
     gene            complement(2697..3071)
                     /locus_tag="PLJ82_07760"
     CDS             complement(2697..3071)
                     /locus_tag="PLJ82_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41435.1"
                     /translation="MFNFGLLASTTPYIVMAFLCAMSYVWMLFDKKPADQIEYISDQQ
                     QLNFDIEHTNKNKATTYVVANDEFKTSPCKYETVDCINTKTNNIVCLSFLTAKLYSKT
                     CLTQLSSIYQHFYFSRPPPFSC"
     gene            complement(3098..>3542)
                     /locus_tag="PLJ82_07765"
     CDS             complement(3098..>3542)
                     /locus_tag="PLJ82_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOR41436.1"
                     /translation="VAFVMTISTIITYLLQKYVLEQFDILFLQTIVFILIISALVQLV
                     EIILKKVSPPLYQALGVFLPLITTNCTILGVAIMTIQKDYTLLEGVVFAISTALGFGL
                     ALVIFAGIREQLVFAKIPKAMQGAPIALLVAGLLAMAFMGFTGIV"
BASE COUNT         1179 a          564 c          577 g         1222 t
ORIGIN      
        1 taccgtaaat atcaataaaa gaaatcccat aattatcatg tctttagaac cttgcttgtt
       61 tcgtattttg ttaaaggcta gtatatttgc caataaaata tgtaacacga aacccaatgc
      121 aaaagtcttt tctagggtat atgctacact attatgttgc ataaaatagt gcattgaatt
      181 gtaataatat ccctgtgtgt tagcaatagc atctagtaca agtaataagt taacagctaa
      241 atgtaataca ataaataaga gcgtgcaaat acctgcaata ctagatatga ttgaaataat
      301 tggtttattc atttttaaaa tctaaaaaaa tacaaagatg caaatttaat caaaaagatg
      361 catctttgta taaatggggt tacaaaatgt gtttcacatg aatgccaaaa gatatcggaa
      421 tacctttttg tacaaaacta tttccaagtg attcaaaata aaacacattg tacgattgat
      481 tgagtgcatt tttgacccaa aatttcagat gtgtattatt ttttgagaat gttatatcgc
      541 catttgcaat accataatat tcttgagaaa gcgtattttg tgcattccag tatagtggac
      601 caatgcctgt gtaatgtgca gaaagaacga tttcatcgag ccattcttcg gttattggta
      661 ccttagtttc aatacctaaa gagagtgtgt ttttaggtgc gaaaggtatt gattttttgc
      721 tgtaattaat gttattcgca ttgtatttaa taaagcttgc atttgtatgt ccgtaatgga
      781 tataccccgc tatttgttta cttaaatatg cctttacact tagttctcca cctatacttt
      841 ggcttacttc tgcattgcta atcatacgac ttccattgct agtggttgta ctcaattgtt
      901 ggttatagtg atgaatataa aaaattgcag tttggtaatt aaaacgattg ttaccgaatg
      961 taccattact accaacctca acattccatg attcttcggg cttataatat attttgtcat
     1021 caatcgttac atctttgtta ccttgcattt gtgcttgtaa aatatcagag aacatagaat
     1081 aattatatcc tccagcttta tatcctttgg ttaatgaagt atatactatg tgttgattat
     1141 ctattttata gtgtaatgca actttcggta atacttctat aaagtctttg cttttaaagc
     1201 cttttacggc atatgttgag tccacccgaa aagcaggaac aaatggggaa ggtaatttta
     1261 tacttgtctg caaggttgct tccgaatcgt agtcgataaa tgctttttca taatccaatc
     1321 gaattccagc ggttacactg aatttattgg tgaatgaata gcttgattgg tggtacaaag
     1381 ctgcaccaat agatggttta ctaaataatc cagcaacaag catttcttca tttaaaatag
     1441 taattgtagg catattggga ttaaacattt ttgccatatc cataaagttt tgtatcatct
     1501 ttataccacc tttttggaat gtcatcggag aatcaatggt attacttgct ccaaataaaa
     1561 ataagccatt tacccattgc caattatttg aattatttga acgaaataca aattcctgcg
     1621 tgatgctctt taaatgttga ttttgttgta ataccataat agagtcggaa gtaaaatctt
     1681 gatctatatc cattctatcc gtaatgtttt gaaaaccagt tgcagaagta aatgcataat
     1741 tatccgatat tttttgaagt gtgaatccag aagataacat tttacgtcta tatgcacctt
     1801 gttcgttgta gttaatagga tatatttgct tgcttattgt gtcaaatttg gcatacgggt
     1861 aggctatttg gtcggtgtaa tcgtaagaaa tttgaaacat tgattgccaa ttgtttttca
     1921 ttttccattc cagttttcct ctaataccaa attcatctga tttatttttc tccattcgtc
     1981 ccatattggt aaaatgtcca tcctggtgct gaacataccc agagatactt acgcccaatg
     2041 aatcgttcaa tttttggtaa tgagatacag acacattggc atcattgtat ttggcatacg
     2101 atacgtttaa ccttgttcct tggtaaacaa gagggcttat tgtatatata ttcatcaatc
     2161 ctccaatcgt atttctgcca tataaggttc cttgcggacc ttgaagaaac tgaaactgct
     2221 gtatatcatg caacataata tcaaaagcac tacgatgaat ataaggcaca ttgtccacat
     2281 acattccaat agcaggttca ttcattctac tccctatacc tctaatatac atggatgacg
     2341 tgatttttga gccataatct ggtaagtaat aattgggaat atgggcagat agatttttta
     2401 ttgattgcac cccaagtttt tcaacttgtt gcattccaat ggtataattt gtagtagcca
     2461 attgtgttgt aaatgctgat tcttttggca aactgtttac aacaaattcg ttgagttcta
     2521 cggtactttt atcgtcagct aaaacgaaaa aggatactag catgagaaga catgcagtac
     2581 taataatttt cataaaaaaa tagtatgtta atgttaataa aatggttgat tgacaaatag
     2641 ttaaatgtca acaaaaaaat aacaactcgt ttcacagagt gtcttttata aatacattaa
     2701 caagaaaagg gaggaggtct agagaaataa aaatgttggt aaatggagga tagttgagta
     2761 agacaagttt tactatataa ttttgcagtt aaaaaagaca aacaaacaat gttattcgtt
     2821 ttagtattga tacaatcaac agtttcatat ttgcaaggcg aagtcttgaa ctcatcatta
     2881 gctactacat acgtagttgc cttatttttg ttagtatgtt caatatcaaa gttaagctgt
     2941 tgttggtcag aaatatattc tatttgatct gcaggttttt tgtcaaataa catccataca
     3001 taagacatgg cacataaaaa tgccataaca atatagggtg tagtagatgc taaaagtcca
     3061 aaattaaaca taatacatac tctttttttt atagaaatta cacaatacct gtaaatccca
     3121 taaatgccat agccaataag cctgctacta gtaaagctat aggtgctcct tgcatcgctt
     3181 tgggtatttt tgcaaataca agttgttctc taataccagc aaatatcact aaagcaagtc
     3241 caaaaccgag agctgtagat atcgcaaaaa caaccccctc aagcaaagtg taatcttttt
     3301 gaattgtcat aattgcaact cctaaaatag tacaattggt ggttattaat ggcaaaaata
     3361 cccccaatgc ttggtataga ggaggagaga ctttttttaa tataatttct actaattgta
     3421 ccagagcaga aataattaag ataaatacaa tggtctgtaa aaataaaata tcaaattgtt
     3481 ctagaacgta tttttgtagc aaatacgtaa taatggttga aattgtcatt acaaaggcta
     3541 cc
//
LOCUS       DAOSRP010000139         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_24230_length_3488_cov_5.748325, whole genome shotgun sequence.
ACCESSION   DAOSRP010000139 DAOSRP010000000
VERSION     DAOSRP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_3488_cov_5.748325"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PLJ82_07770"
     CDS             complement(<1..1391)
                     /locus_tag="PLJ82_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41437.1"
                     /translation="MTDKEIVLTKENLFYYLSKSQLGRAIELIDNLLTQVQDGYYGDE
                     VYELKTNYKRMLQYMFYGVNDPEQHLVYQHLLKETYEIGEHLADKLLQLNSTQYTYRT
                     KRLFAVQPVISDELVLSGFQRYASLGNRKTESDIREYEMLISYSFDKYWLTSLYSETM
                     LQQVKLLAEDETIGQIELCSLASAITLSSMAFFDEKKCLALVELTKSRYPEVVLRALT
                     GVVLFLYFHHQRISFYPVIRTQITLLFDKEEFIVASQLIIKQFIRSKETEKITKDITE
                     NILPELSKIAPHVQNDLADIDKQEELEDKKNQWQDMIEETGIGEKMQQYAELQSQGAD
                     IQMSTFSMLKNYPFFNVVSNWFLPFYAEHSSVSNLFKEEKENSFLSFLAKSPILCHSD
                     KYSFCLNLTMVPEQYRKAMMNGVQMESEQLKEMTNDKLSLSNTVVAEEIMNKYIQDLY
                     RFYKLFPKNNKNS"
     gene            1602..1847
                     /locus_tag="PLJ82_07775"
     CDS             1602..1847
                     /locus_tag="PLJ82_07775"
                     /inference="COORDINATES: protein motif:HMM:NF026249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOR41438.1"
                     /translation="MIQQPSSSHFSFIKDGILYIKRSKMAKILLIIILIKFLIFYGFL
                     KGFLYPRYLKPKWESEQHRIESVTHDLIQPLNTISYD"
     gene            1840..3396
                     /locus_tag="PLJ82_07780"
     CDS             1840..3396
                     /locus_tag="PLJ82_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOR41439.1"
                     /translation="MIENIDLTLVNWSRAQFAMTAFFHWLFIPLTIGMTYIIAFAQTL
                     HVRTNDPEWEKLAKFWAKLFGINFAIGVGTGIILGLEFGTNWSNYSWISGDIFGAPLA
                     AEGIMAFFLESTFVAVMFFGWGRISKKFHLVACYLVAFGASLSALWILTANAWMNYPV
                     GMSFNPDIARHEMVDFWAILFSPFAVNKFLHTISSGFVIGSFFIIAVSSWYLLKGRSV
                     LFAKRSIFLASVFGLVGTGLVILTGDGSAYNVAQHQPAKLAAMEGLYDGKEGAGLVTV
                     GILTPGKKIGDNKDPFIFKQEIPYVLSFLGYRDINAFVPGINDLVYGYKSDKLEIVSA
                     SKKMEYGRIAMQALSLYKKTDNSEEKTMALQLFTEYQPFMGYAHITSPEKVIPNVPMI
                     FYSFHIMVMAGFFLIAVVAGLLFFSIKNKVENHKWLLRLGILGFPMAFIASMTGWIVA
                     EVGRQPWTIQDLLTTSVSTSNINSMAVILTFWMFFALLSVLVIAEVRIMLAAIKKGPE
                     STNTKGGENA"
BASE COUNT         1067 a          615 c          597 g         1209 t
ORIGIN      
        1 aaactgtttt tgttattttt agggaacagt ttgtaaaaac gatataaatc ttgaatgtat
       61 ttattcatta tttcttcggc aacaaccgta ttagatagag atagcttatc gttcgtcatt
      121 tcttttaatt gttccgattc catttgtaca ccgttcatca ttgctttgcg atattgctct
      181 ggcaccatcg ttaaatttag gcaaaacgaa tatttatccg aatgacaaag tataggactt
      241 ttggctaaaa aagataaaaa actattttcc ttttcttctt taaataagtt gctcacagaa
      301 gaatgttcgg cataaaaagg caaaaaccaa ttggaaacaa cattaaaaaa tggataattt
      361 ttgagcatcg aaaacgtact catctgaata tctgcacctt gactttgtag ttcggcatat
      421 tgttgcattt tttcgccaat acccgtttct tctatcatat cttgccattg atttttttta
      481 tcttccaact cttcttgctt atcaatatcg gctaaatcat tttgcacatg tggagctatt
      541 ttggataatt ctggcagaat attttcagta atatcttttg ttattttttc agtttcttta
      601 cttctaataa attgttttat aattaattgt gaggctacga taaactcttc tttatcaaac
      661 aacaaggtaa tttgggtacg aataacggga taaaaagaaa tgcgttgatg atgaaaatac
      721 aaaaataaca ctacgcctgt caatgccctt aatacaactt caggataacg cgattttgtt
      781 aattcaacta acgccaaaca ttttttttcg tcaaaaaaag ccatgctact caatgtaatt
      841 gctgaagcaa gtgaacacaa ttcgatttga ccaatagtct catcctctgc caacagtttt
      901 acctgttgta gcatagtttc gctatacaga gatgttaacc aatacttatc aaaactataa
      961 gaaatcaaca tttcatattc acgaatatca ctttccgttt tacgattccc tagtgatgca
     1021 tagcgttgaa aaccagacaa taccaactca tctgagataa caggttgtac ggcaaataaa
     1081 cgtttggtac gatatgtata ttgggtacta ttaagttgca aaagtttgtc agctaaatgc
     1141 tctcctatct cgtacgtttc tttcagtaaa tgctgataaa caagatgttg ttcaggatca
     1201 ttaaccccgt aaaacatata ctgcaacata cgtttatagt ttgttttaag ttcgtaaact
     1261 tcatcgccat aataaccatc ttgcacttgt gtcaacaaat tatcaatcag ctctatagct
     1321 cttcccaact gggatttact caaataataa aacaaatttt ctttcgttaa aacaatttct
     1381 ttgtctgtca tatggttgat gtaaattctg taaatcaaat gtaagtgaac aaagatacac
     1441 aatttgcaaa aaaaaatgtc atacgatagg attgaatata cagtatattt cgcaaattat
     1501 tgttttttag ttaaaaatca acttcaagac acccattttt gtaataacta tttgattttt
     1561 ttatcatttc ttaatatatt tgctacctat tgaacactta tatgatacaa caaccaagtt
     1621 ctagccattt ttcttttatc aaagatggca ttctatacat caaacggtca aaaatggcta
     1681 aaatattgtt gataatcatt cttatcaaat ttttaatttt ttatggattt ctaaaaggtt
     1741 ttttgtatcc tcgttatctt aaacccaaat gggaaagtga acaacaccgc atcgaatcag
     1801 ttactcatga tttaatacaa ccattaaaca ccatttcgta tgattgaaaa cattgactta
     1861 acactggtaa actggtcgcg agcccaattt gccatgacag ccttttttca ttggcttttt
     1921 atacctctta ccattggtat gacgtatatc attgcctttg cacaaaccct ccacgtaaga
     1981 accaatgacc ctgaatggga aaaactcgca aaattttggg caaaattatt tggtatcaac
     2041 tttgctattg gtgtgggcac aggcatcatt ttgggtttag agtttggcac caactggtct
     2101 aattattcgt ggatttcagg cgatattttt ggtgcaccct tagcggctga aggtattatg
     2161 gcattctttc tcgaatctac ttttgttgct gttatgtttt ttggttgggg gcgtattagc
     2221 aaaaagttcc atttagtagc ctgttatctg gttgcctttg gtgccagttt atctgcccta
     2281 tggatactaa cagcaaacgc atggatgaat tacccagtgg gcatgagctt taacccagat
     2341 atcgctcgcc acgaaatggt agatttttgg gcaattttat tttcaccttt tgctgtaaat
     2401 aaatttttac acaccatctc ttcgggtttt gtaattggct ctttctttat tattgctgta
     2461 agtagttggt atttgctaaa aggacgcagc gtactgtttg caaaacgtag tatttttcta
     2521 gccagcgtat ttggattagt gggcacaggc ttggttatct taacaggcga tggttcagca
     2581 tataatgtag cacaacatca acctgcaaaa cttgccgcca tggaaggatt gtatgatggc
     2641 aaagaaggag caggattggt aactgtagga atacttacac ctggtaaaaa aattggagat
     2701 aacaaagacc cgtttatttt caaacaagaa ataccttatg tcctttcatt tttgggctat
     2761 agagatatca atgcatttgt gcctggcatc aatgatttgg tatatggata taaatcagac
     2821 aaattagaaa tagtatctgc ttctaagaaa atggaatatg gaaggatagc aatgcaagcc
     2881 ctctcgttgt ataaaaaaac ggataatagc gaagagaaaa ctatggcttt gcaattattt
     2941 accgaatacc aaccttttat ggggtatgct catattacaa gtcctgaaaa agttattcca
     3001 aatgtaccaa tgatttttta tagtttccat attatggtga tggcgggctt tttcttgatt
     3061 gctgttgtag ctggattgtt gtttttcagc atcaaaaata aagtagaaaa ccacaaatgg
     3121 ttgttgcgat tgggtatcct tggtttcccg atggcgttta ttgcctctat gacaggatgg
     3181 atagttgccg aagtgggtcg ccaaccatgg acaatacaag atttgctgac cacatctgtt
     3241 tctacatcta acatcaattc aatggctgta attcttacgt tttggatgtt ttttgcactg
     3301 ctttctgtgc ttgtaatagc cgaagttcgc atcatgttag ctgctatcaa aaaaggtcct
     3361 gaatcaacta acacgaaagg aggggaaaat gcttaattca ttatcacact ttgatttaca
     3421 acaatactgg tggcttataa tgtcgcttgt aggttcaata ctcgtttttc ttctttttgt
     3481 acaaggag
//
LOCUS       DAOSRP010000140         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_24407_length_3468_cov_9.229710, whole genome shotgun sequence.
ACCESSION   DAOSRP010000140 DAOSRP010000000
VERSION     DAOSRP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24407_length_3468_cov_9.229710"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLJ82_07785"
     CDS             <1..268
                     /locus_tag="PLJ82_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOR41440.1"
                     /translation="IPDGFSPNGDAINDTFVIQRSSNLTINLIVFNRWGNKVFEQANY
                     ANDWEGTGINNQALPEGTYYRIVEAINNTTGQTQKLSGVITLKR"
     gene            292..1215
                     /locus_tag="PLJ82_07790"
     CDS             292..1215
                     /locus_tag="PLJ82_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOR41441.1"
                     /translation="MKKILFIFFACIAGYVVAFAQQAPMYSQYMFNMLNVNPAYAGNR
                     AVQNVTLLHRHQWVGFEGNPRTSSLSWDMRAEDSNNGFGAQIYNDQLGVENTTGLQGF
                     YSYKIPLKNASFALGLSAGLLNFSAAYTEVTTIDPNDPAFAENANTFLPTVGVGLLYA
                     SEKWYLGLSVPALLRTKDFLAEAEALVNKFGATNHYFITGGYVVEVNPNLALKPSLLF
                     KAVSGAPLQTDININAWWNQKWGAGASFRTGDAVVAMLEYQITNTIRLGYAFDYSISQ
                     LNAFNQGTHEIMLRYEFDTSKAIRVLSPRYY"
     gene            1263..3467
                     /locus_tag="PLJ82_07795"
     CDS             1263..3467
                     /locus_tag="PLJ82_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOR41442.1"
                     /translation="MMKNISYKIRLTLACVLLSATSVQLIAQTNNDILKQADRHYQYA
                     NYARAVQLYEQVVAQTKLLVTQEATPEQTEKLQQEKIDLFTKIADSYWQMRQYNKAKV
                     ALDEIKNISLVKLSNKEKNRLAQLYALTGDYKKAADIYDQIPGAKNKAELYRQDDFFG
                     KLKADSADWTIQLLNINSAYNDFSPVIVHQNILFSSNKPQGKEKRAFGWDGNDYTSLW
                     LFPIKDLKNIQEKPTQKSDKKTTTLQTKKIAGIYTPGDVRPMEQRSSLKNEMPLLPTT
                     QSGKGVRVSGAEKFQFNIATASVDSLGNIFLSVNNQKVKNTQTSSISIMQGSWANQAI
                     SQVKALPLDGFFATMHPTVNKQSTLLIFTAKIAESDSYDLYMSKRNDPNQAWSVPVKL
                     AENINSTGNEVFPYIAPDGYLYFSSDGHPGLGGLDLFKIDLQQAINKTGTIESLGYPV
                     NSAADDFGWAQDTTKRAGFFSSNRYANTDNIFSYAYLPIPKSMFVSGLVKEKKSLDLL
                     PGATVFLVDKEKQIVYVDKADKDGRFTFEIKNHGDFTLKGLEENYFEDCIDINVPAPA
                     YKQNQYVAKDILLELQVPKKWVLENLLYDLDKWNIRKDAQAPLDSVVAIMKKYPIFVE
                     LGSHTDSRGSDAYNERLSQRRAQSAVDYIVSKGISRSRITAKGYGETQPTNECKNGVN
                     CSEEQHQANRRTEIKVTHNPEPAHTLDPNKFKKGDILKTSDFPEGFFGKCRE"
BASE COUNT         1125 a          720 c          691 g          932 t
ORIGIN      
        1 tatccccgat ggtttttcac ccaatggcga tgctatcaac gatacgtttg tgatacaacg
       61 tagctccaac ttaaccatca accttattgt attcaaccgc tggggcaata aagtgtttga
      121 gcaagctaac tatgccaacg attgggaggg taccggtatc aataaccaag cactacccga
      181 aggaacttac taccgtattg tagaagctat aaacaatact actggacaaa cacaaaaact
      241 atctggtgtt attactctta aaagataaga atcgactgaa atttactaat tatgaaaaaa
      301 atactattca tcttctttgc ttgtatcgct ggttatgttg tggcttttgc tcagcaagct
      361 cccatgtact cgcagtatat gtttaatatg ctcaacgtga accctgccta tgccggcaac
      421 agggcggtgc aaaacgtaac gcttttacac aggcaccaat gggtgggatt tgaagggaat
      481 cctcgcacat catccctttc gtgggacatg agggctgaag attctaataa tgggttcgga
      541 gctcaaattt ataacgacca actaggtgtg gaaaacacca ccgggttgca gggattttat
      601 tcctacaaaa tacctttaaa aaacgcttct tttgcccttg ggttaagtgc tggattgctt
      661 aatttctctg ccgcttacac agaggtaact accatagacc cgaatgaccc tgctttcgct
      721 gaaaatgcaa atacattcct tcctaccgta ggtgtggggc tattgtatgc ttccgaaaaa
      781 tggtatctag gactctctgt tcctgccctg ctcagaacaa aagatttctt ggctgaagca
      841 gaagctctgg taaataagtt tggggctact aatcactatt ttattacggg ggggtatgtt
      901 gttgaggtaa atccaaattt agctctcaaa ccatcgctat tattcaaagc tgtttcgggg
      961 gctcctttgc aaacagacat taacatcaat gcttggtgga atcaaaaatg gggggctggg
     1021 gctagtttcc gtactgggga tgccgttgta gcaatgctcg aataccaaat aacaaacacc
     1081 attcgtttgg ggtatgcttt cgattactct atttcccaac taaatgcttt taaccaaggt
     1141 acacacgaga ttatgctccg ttacgaattc gatactagca aagccataag ggtattatcg
     1201 cctcggtatt actaacaggg tgcttactta cattgtataa cgtctttaat tatttactaa
     1261 tcatgatgaa aaatatatcc tacaaaataa gactaacgct agcctgtgta ttgctttctg
     1321 ctactagtgt acagcttata gctcaaacaa acaacgacat tctcaaacaa gctgacaggc
     1381 attaccaata tgctaattat gctagagcgg tgcaactata cgaacaggtg gtggcacaaa
     1441 cgaagctgct ggtaacccaa gaggctacgc ccgaacaaac agaaaaattg caacaagaaa
     1501 aaattgacct gtttaccaaa attgctgata gctactggca aatgagacaa tacaacaagg
     1561 ctaaagttgc tctcgatgaa ataaaaaata taagccttgt gaaactatcg aacaaagaaa
     1621 aaaacaggct cgctcaactc tatgctctta caggtgatta caaaaaagct gccgatattt
     1681 acgaccaaat tccaggggcg aaaaacaaag ctgaactcta tagacaagat gatttctttg
     1741 gcaaactaaa agccgactct gccgattgga ctatccagct ccttaatatt aacagtgcat
     1801 acaacgattt ctcccctgtc attgtacatc aaaacatcct tttctcttct aacaaacccc
     1861 aaggtaaaga aaaaagggca ttcggatggg atggtaatga ttacacctct ttatggttat
     1921 tccctataaa agacctaaaa aatatacaag aaaaacccac tcaaaaaagt gataaaaaga
     1981 caacaacgct acaaacaaaa aaaatagctg gcatatatac ccctggtgat gtgcgtccta
     2041 tggagcaacg cagttccctc aaaaatgaaa tgcctttgct tcctactact caatcgggga
     2101 aaggtgtgcg tgtgagtggt gccgaaaaat tccaattcaa tatagcaact gcaagtgtag
     2161 attctttagg aaatattttc ttatctgtta acaaccaaaa agtaaaaaat acacaaacca
     2221 gttctatttc cattatgcaa ggttcgtggg ctaaccaggc tatatcgcag gtaaaagctc
     2281 tgcctttgga tgggtttttt gctaccatgc accctaccgt taacaaacaa agtaccctcc
     2341 ttatttttac tgctaaaata gcagaatcag actcatacga cttgtatatg agcaagcgta
     2401 atgaccctaa tcaagcatgg agtgtgcctg taaaattggc tgaaaacatc aactctacgg
     2461 gaaatgaggt gtttccttat atagctcctg acggatactt gtattttagc tcagacgggc
     2521 atcctggatt aggggggttg gatttattta aaatagactt acaacaagct atcaataaaa
     2581 ccggtactat tgaatccctc ggatatcctg ttaactctgc tgctgatgat tttgggtggg
     2641 ctcaagatac cactaaaagg gctgggttct tctcctctaa taggtatgct aatacagata
     2701 atattttttc gtacgcctac ttacctattc ctaaaagtat gtttgtgtca ggattagtga
     2761 aagagaaaaa atccctcgat ttattgcctg gggctactgt atttttggtg gacaaagaaa
     2821 aacagattgt ctacgtggac aaagcggaca aggatggtcg tttcacgttt gagataaaaa
     2881 atcatggcga ttttacgcta aagggattag aagaaaacta ttttgaagat tgtatcgaca
     2941 ttaacgtgcc ggctcctgct tacaaacaaa atcaatatgt ggcaaaagat attttgctcg
     3001 agttgcaagt acctaaaaaa tgggtgttag aaaatctatt gtacgacctc gataaatgga
     3061 acatacgcaa agatgcacaa gctcctttgg acagtgtggt ggcgattatg aaaaaatacc
     3121 ccatttttgt agaactgggt tcgcatactg acagtcgtgg gtccgatgcg tacaacgaac
     3181 gtttatccca acgcagggct caatcggcgg tcgattatat cgtgagcaaa ggaatttctc
     3241 gctcacgtat tacagccaaa gggtatggcg aaacacaacc aaccaacgaa tgtaaaaacg
     3301 gcgtaaactg ttccgaagag caacaccaag caaacagacg tacagaaata aaggttacac
     3361 ataacccaga gcctgctcat acgcttgacc cgaacaaatt taaaaaagga gacatactca
     3421 aaacatccga cttccctgaa gggttcttcg gaaaatgtag ggaataaa
//
LOCUS       DAOSRP010000141         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_24733_length_3433_cov_4.966252, whole genome shotgun sequence.
ACCESSION   DAOSRP010000141 DAOSRP010000000
VERSION     DAOSRP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24733_length_3433_cov_4.966252"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..449)
                     /locus_tag="PLJ82_07800"
     CDS             complement(147..449)
                     /locus_tag="PLJ82_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41443.1"
                     /translation="MKTTGEILRESREKKGMLLRHLSATLEIDTAILSKIERGERKAT
                     REQINKLAEILEIDKEYLMIQFLSERILYELRNEDLGIKALKVAENAIKYGGIKNK"
     gene            694..1035
                     /locus_tag="PLJ82_07805"
     CDS             694..1035
                     /locus_tag="PLJ82_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOR41444.1"
                     /translation="MKKTEKFDVELQEIAEFARVISHPARLAILKYLAETKTCISGDI
                     SDQLPLSRTTVSQHLKELREIGLIHGEIDGIKINYCLCSATIQQQLQRFEAFFSPIKE
                     SASSCEMSSCK"
     gene            1068..1493
                     /locus_tag="PLJ82_07810"
     CDS             1068..1493
                     /locus_tag="PLJ82_07810"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOR41445.1"
                     /translation="MRVLILCTGNSCRSQMAHGFLQSFDKSITVCSAGTEASGKLNQK
                     AVKAMAEIGIDISHHTSDSVSKYLGEEWDYVITVCGGANEACPAFLGKVKNRLHIGFD
                     DPSHAVGSEEFIWSEFIRVRDEIKEGFYAFYMEKIKPQL"
     gene            1501..2550
                     /gene="arsB"
                     /locus_tag="PLJ82_07815"
     CDS             1501..2550
                     /gene="arsB"
                     /locus_tag="PLJ82_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOR41446.1"
                     /translation="MNKEAEKMSFFDRYLSIWVAICIVVGIAMGKLLPIVPETLGQLE
                     YANVSIPIAVLIWIMIFPMMLKIDFKSVVDAVKMPKGLTITLVVNWLIKPFTMFGIAW
                     LFFMVIYSLWIPEDLAKEYLAGAVLLGAAPCTAMVFVWSHLTKGNPAYTLVQVAINDL
                     IIIVAFIPIVTLLLGVSGIVIPWNTLIMSVLLFVVIPLVLAYITRNWVIRKKGIAYFT
                     DVFIKKFSATTISGLLLTLVIIFTFQGDVILQNPLYILLIAVPLIIQTFFIFFVAYRW
                     AKAWKLPHDIAAPASLIGASNFFELSVAVAIVLFGLQSGATLVTVVGVLVEVPVMLAL
                     VKIANKTKHQFPSKQ"
     gene            2625..>3433
                     /locus_tag="PLJ82_07820"
     CDS             2625..>3433
                     /locus_tag="PLJ82_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOR41447.1"
                     /translation="MTLINKINIGIDAGSTTVKIVVTNQHHALLYKEYVRHKADVAGT
                     IVTSLLPLLHTIGNKDVSVTITGSAGMGIAERCSLPFVQEVVASCELIQTLYPIVKTL
                     VDIGGEDSKMIFFSPNHAPDMRMNGNCAGGTGSFIDQIATLLGVGVHELDELAQAATS
                     VYPMASRCGVFSKTDVQNLLSRNVSKQDIALSIFHAVSLQVISSLSKGNTIEAPLFLS
                     GGPFAFIPTLKKVFMQQLSINEAQIIDSENRQFIPAWGAAIESNQQTSTLA"
BASE COUNT          990 a          607 c          654 g         1182 t
ORIGIN      
        1 taacaacttg aatagaacct ttgtctccca ttttttttac cctaccatat tgtataaggt
       61 atgagatatt tgatgttgta acattttttt ccaaatactt tgatgcccat tggcttgcct
      121 cttttattga taataattct gccattttat ttatttttta ttcctccata tttaatagca
      181 ttctcggcaa cttttagagc ctttattcct aaatcttcat ttctaagctc atacaaaatt
      241 ctttcgctta aaaattgaat catcaaatat tctttatcta tttcgagaat ttcagctagc
      301 ttatttattt gctctcttgt tgcctttctt tctcctcttt ctattttact tagtatagct
      361 gtatcaatct caagagtggc agataggtgc ctcaataaca tgcctttctt ttctcgactt
      421 tctcttaata tttctccagt tgttttcaat atgaaacacc ttaatttgac aatttttgtc
      481 aaatgtagca acattttttg atttatcaca atgaatgtca aattttttat aaacaatatt
      541 tgtaactgtg atatgtaaat caaaatcaca tcctcttaac ccgtttttta caaattgttc
      601 ttacctaaac acccattctt cacaaaaacg taacacccat aattttgata tctaagattt
      661 atgtcgtatg tttgcgatat aaaacataat taaatgaaaa agacagaaaa atttgatgtt
      721 gaattgcaag agattgctga gtttgcacgc gtaatatcac atcccgctcg cttggctata
      781 ttaaaatatt tggcagagac gaaaacatgt atttcggggg atatttcaga tcaactgccg
      841 ttgagcagaa caaccgtatc gcaacactta aaagaattac gcgaaatagg cttgatacat
      901 ggcgaaattg atgggataaa aattaattat tgtttgtgta gtgctactat ccaacaacaa
      961 ttgcaacgtt tcgaggcttt cttttctcct atcaaagaga gtgctagcag ttgcgaaatg
     1021 tctagttgta aataaatata ttcacttaaa taataacaga aaaacacatg agagtattga
     1081 ttctttgtac aggaaatagt tgccgtagcc aaatggctca tggctttttg caatcgttcg
     1141 acaagtcaat tacagtttgc tcagcaggaa cagaagccag tggaaaactc aatcaaaaag
     1201 ctgtaaaagc aatggctgaa attggcattg acatcagcca ccatacttcc gattcggtaa
     1261 gtaagtattt gggagaagaa tgggattatg tcattactgt atgcggtgga gccaacgagg
     1321 cttgtcctgc ttttttagga aaggtgaaaa atcgtttgca tattggtttc gacgatccaa
     1381 gtcatgctgt agggagcgag gagtttattt ggagcgaatt tatacgtgtt cgcgatgaaa
     1441 taaaagaggg attttacgct ttttatatgg aaaaaattaa accccaattg taaggttgtc
     1501 atgaacaaag aagctgaaaa aatgtctttt ttcgaccgtt acctctccat ttgggtagca
     1561 atctgtattg tggttggtat tgctatgggc aaattgttac caatagtacc cgaaacctta
     1621 ggtcaattag agtacgccaa tgtgtctatt cccattgctg tgcttatttg gataatgatt
     1681 tttccgatga tgctcaaaat tgactttaaa agtgtggtag atgcagtcaa aatgcccaaa
     1741 ggtcttacca ttaccttggt ggtaaactgg ctcatcaaac catttactat gtttggtatt
     1801 gcctggttgt tttttatggt tatttattcg ctgtggattc ccgaagacct tgcaaaagag
     1861 taccttgcag gtgcggtatt gcttggtgcg gctccgtgta cggcaatggt gtttgtgtgg
     1921 agtcatttaa ccaaagggaa tcctgcttac accttggtac aagtagccat caacgattta
     1981 atcattattg tagcttttat tcctattgta acgcttttat taggcgtttc tggtatcgtt
     2041 attccgtgga atacgctcat tatgtcggtt ttgttgtttg tagtcattcc cttggtgctt
     2101 gcttatatca cacgcaattg ggttatccgc aaaaaaggta tcgcctattt tactgatgtg
     2161 tttatcaaaa agttttctgc cactaccata agtggtttat tgcttacttt ggtaatcatt
     2221 tttacatttc aaggggatgt gattttgcaa aatcctttgt atattttatt gattgcagta
     2281 cctcttatta tccaaacatt ctttattttc tttgtggcat atcgctgggc taaagcatgg
     2341 aaactaccgc acgatattgc tgctccagca tcgcttattg gtgctagtaa tttttttgaa
     2401 ctatcggtgg cagtagccat tgtgttgttc ggattgcagt cgggagctac tttggtaaca
     2461 gtagtaggcg tattggtaga agttcctgta atgctggcat tggttaaaat tgctaataaa
     2521 acaaaacatc agtttccaag caagcaataa aaagaatatc ttgctatttt tttgtacatt
     2581 tgtggcgatt tttggaaaat atgccatatc cacaaaaaag gaagatgacg ttaatcaaca
     2641 aaattaatat aggtattgat gctggttcaa ctacagtaaa gattgttgta accaaccagc
     2701 atcatgcttt gttgtataaa gaatatgtgc ggcataaagc cgatgttgct ggaacaattg
     2761 taacttcatt attgccacta ttgcatacca ttggcaacaa agatgtttct gtaaccatta
     2821 ccggttcggc tggtatgggt atagctgagc gttgtagttt gccttttgtg caagaagtag
     2881 tggcatcttg cgaactgatt caaaccttat accctatcgt aaaaacattg gttgatatag
     2941 gcggagaaga ttctaaaatg atattttttt cgcctaatca tgctcctgat atgcgtatga
     3001 atggcaattg tgctggagga acgggttcgt ttatcgacca gatagcaacg ctgcttggtg
     3061 taggtgtaca cgaattagat gaattggcac aagctgctac atcagtctat cctatggctt
     3121 ctcggtgtgg tgttttttcc aaaacagatg ttcaaaacct gctaagtcgc aatgtgagca
     3181 aacaagatat tgccttatcc atatttcatg cggtatcgtt gcaagtaata tcgtctttat
     3241 ccaaaggaaa taccatagag gctccgttat ttttatctgg tggtccgttt gcttttattc
     3301 ctaccttgaa aaaggtgttt atgcaacagc tgtcaatcaa tgaggcacaa ataattgatt
     3361 cagaaaacag gcaattcata cctgcatggg gtgctgcaat tgaatcaaat caacaaacat
     3421 ccacactcgc att
//
LOCUS       DAOSRP010000142         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_24880_length_3418_cov_4.618793, whole genome shotgun sequence.
ACCESSION   DAOSRP010000142 DAOSRP010000000
VERSION     DAOSRP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24880_length_3418_cov_4.618793"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3418)
                     /locus_tag="PLJ82_07825"
     CDS             complement(<1..>3418)
                     /locus_tag="PLJ82_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOR41448.1"
                     /translation="NQQTSTLALSALIKKIEQSKQQVYTYNPTALPPLFSDNEHYLRW
                     KKEKESSCVIETIDIADATEGCYIGIDSGSTTTKIVALDEKKRVFYRFYERNSGSSLD
                     TVQKGLNSLLQQANEANKNLMVLGSCVTGYGEDLVKNAFPIGLGMVETLAHFTASVHF
                     NPHVSFILDIGGQDMKACFIENGAIKRLDINEACSSGCGSFIETFAHSLGHTAASFSD
                     LCLQSKLPYDLGTRCTVFMNSKVKQALREGAPMADIAAGLGYAVIKNCLNKVLKINDF
                     STLGKHIMVQGGTFRNIAVLRSLEIETGSNVMITNMPELMGAFGAALFALKNREKQQP
                     TNLQDCVQIRTYTCQTLLCKGCENNCLVTRYQFGKESKFYAGNKCEKVFSNRGNADIK
                     GKNAFDYKYQLLFNRPHAKNPVLKLGIPRALGMYENYPFWHGLFHACGIEVVLSDTST
                     LKLYEKGAHTVMADNICFPAKLTNGHIVNLMEKKPDRIFLPFVVYENQSDTKAPNSYN
                     CPIVTAYSEVIRSAINPMSSSGIPFDSPSVNFKDKKLLKQACDTYLLSILPHLTKKDL
                     KNAFEYAFKQQVIFEQTITDYCQTIATKADTEKRFLVVLGSRPYHTDPLVQHKIADVI
                     ADFGVDVITEDIARMAAKKLPKVHMVMQWAYTNRILKAATWVANKGEHVQYMQLTSFG
                     CGPDAFIIDEVNTLLQQVSKNPTILKIDDINNIGSTRLRIRSVIESLGLSSQAVQSTS
                     TENSSVPFFEKKDRKRKILLPWFADFYSPFIPPIFHRLGYEAENLPPSDMESVAYGLK
                     YSNNEICYPATLVVGDCMKALASGKYKREEIALGISQTGGQCRATNYISLMKKAMLSA
                     GYDDIPVISVGVGGTGVLNEQPGFEVKWHKVLKLIIATMGYADCLSQLYYSTAPRERV
                     QGAAERLKNKYISLAVEAVEKHQTRVLNELLVQAVKEFSDSNSFRDIPRMGIVGEIYV
                     KYNNFGHKNITHWLISQGIEPVLPPLSAFFTETFASKKARIVGNIEHKNNKQFIMNWA
                     ERYVFSILHKMERCMVDYPYHIPLTNPHHDALKASKIINLNTQFGEGWRIPAEFAHFA
                     EHGINHVISLQPFGCIANHIISKGIEKRTRELYPKLQLLQLDFDSSMGEAN"
BASE COUNT          996 a          708 c          620 g         1094 t
ORIGIN      
        1 attggcttcg cccatgctac tgtcgaaatc gagttgcaat aattgtagtt taggatacaa
       61 ttcgcgggta cgtttttcga tacctttgga aatgatgtga ttggcaatgc atccaaaagg
      121 ttgcaaacta ataacatggt tgataccgtg ctctgcaaaa tgagcaaatt cggcaggaat
      181 acgccatcct tcgccaaatt gggtattcaa attgataatt ttcgaagctt ttagggcatc
      241 gtgatgagga ttagttaatg ggatatggta tggataatca accatacaac gttccatttt
      301 atgcaaaata ctaaacacgt atctttcagc ccaattcatt atgaattgct tgttgttttt
      361 atgctcaata tttcctacga tacgagcttt tttgctagca aaggtttcgg taaagaatgc
      421 acttaatgga ggcaatacag gttctatccc ttgactaata agccaatgtg taatattttt
      481 atgaccaaag ttattgtatt taacataaat ctcacctaca atacccatgc gtggaatatc
      541 cctaaaagag ttgctatctg aaaattcttt tactgcttgt accaacaact cattcaacac
      601 cctagtttga tgtttctcca ctgcctctac ggctaatgaa atgtatttgt ttttaaggcg
      661 ttcagctgct ccttgcacgc gttctctggg ggctgttgaa tagtataatt gcgacaaaca
      721 atcggcatac cccatggttg caataatcaa cttgagcact ttgtgccatt taacttcaaa
      781 accaggttgt tcgttgagca cccccgtacc tccaacgcca acagatataa ctggaatatc
      841 atcatatcca gctgataaca tggctttttt cattagcgaa atatagttgg ttgccctaca
      901 ctgaccacct gtttgtgata tccccaaggc aatttcttcc cttttgtatt tgccactagc
      961 caaagccttc atgcaatcgc ccacaaccaa ggtagcagga taacaaattt cgttgttgga
     1021 atattttaat ccatacgcaa ccgactccat atcgcttggc ggtaggtttt ctgcctcgta
     1081 gcctaagcga tgaaaaatgg gcggaataaa tggcgaataa aaatcggcaa accaagggag
     1141 taagatttta cgttttctat ctttcttttc aaaaaatgga acacttgagt tttcagtact
     1201 tgtgctttga acagcttgac ttgacagacc caaactttct atcacagagc gaatacgcaa
     1261 acgcgtagaa ccgatattgt tgatatcatc aattttcaga atagttgggt ttttgcttac
     1321 ttgttgcaat aatgtattta cctcatcaat gataaacgca tcgggtccgc aaccaaaaga
     1381 tgtaagttgc atatactgca catgttcacc tttattggct acccaggttg cagcttttaa
     1441 tattctattc gtataagccc attgcattac catgtgcact ttgggcagtt ttttggctgc
     1501 catcctagca atatcttctg taatcacatc cacaccaaaa tcggcaatca catcagcaat
     1561 tttgtgctgc accaatgggt cggtatggta gggacgcgag cctaatacca caagaaaacg
     1621 cttctcagta tcagcttttg ttgctattgt ttggcaataa tcagtaatgg tttgttcaaa
     1681 aattacttgt tgcttaaacg catattcaaa ggcgtttttc aagtcttttt ttgtaagatg
     1741 aggcaatatc gacaataagt aggtatcaca agcttgtttc agtagttttt tatccttaaa
     1801 atttacactt ggcgaatcaa atggaatacc gctactagac ataggattga tagcactacg
     1861 tatgacctcc gaataggctg ttacaatggg gcaattgtag ctgtttggag ctttcgtatc
     1921 cgattggttt tcgtacacca caaatggcaa gaaaatacga tcaggctttt tctccatcaa
     1981 gttaacgata tgtccgttgg ttaactttgc agggaaacaa atattatcag ccattactgt
     2041 gtgagcccct ttttcatata attttaaggt agatgtatcc gacaatacca cctcaatacc
     2101 acaggcatga aacaagccat gccaaaaagg ataattttcg tacataccca aggcacgcgg
     2161 aatacctagt ttcaacacag gatttttagc atgaggacga ttaaacaaaa gttggtattt
     2221 ataatcaaac gcattcttcc cttttatatc agcattacct ctgtttgaaa acactttttc
     2281 gcatttattt ccagcataga acttactttc tttgccaaat tgataccgag taaccaaaca
     2341 attattttcg caccctttac acagtaatgt ttgacaggta taggtgcgaa tttgtacgca
     2401 atcttgtaag ttagttggtt gttgtttttc tctgtttttc agggcaaaca aagctgcacc
     2461 aaatgccccc attaattcag gcatattggt aatcatcaca ttgctgccag tttctatttc
     2521 tagcgaacgc aatacggcga tatttctaaa cgtgccacct tgcaccatga tgtgtttgcc
     2581 aagtgttgag aaatcattga tttttaacac cttgtttagg cagtttttaa taacagcata
     2641 tcctaaacca gcggctatgt cagccattgg agctccctcg cgtaaagctt gtttcacttt
     2701 agagttcata aaaacggtac accgtgtacc caaatcataa gggagtttgg attgcaagca
     2761 caagtcagaa aaagaggcag cggtatgtcc caacgaatgt gcaaatgttt cgataaacga
     2821 accacacccc gaagaacaag cctcattgat gtccaaacgt tttatggcac cattttctat
     2881 aaaacaggct ttcatgtctt gcccaccgat atctaaaata aaacttacgt ggggattaaa
     2941 atgtactgaa gctgtaaaat gagccaaggt ttctaccatt cccaatccga tgggaaaagc
     3001 attttttact aaatcttcgc catagcctgt tacacaactt cccaagacca ttaaattttt
     3061 attggcttcg ttggcttgtt gcaacaatga gtttaagcct ttttgtaccg tatccaacga
     3121 acttccacta ttgcgttcgt aaaaccgata aaatacccgt tttttttcat ccagggcaac
     3181 aatcttggtt gtggttgaac cgctatcaat tccaatataa cacccttcag ttgcatcggc
     3241 tatatcaatg gtttcaatta cacaactact ttctttttct tttttccacc tcaaataatg
     3301 ctcattatct gaaaataaag gaggtagtgc tgttggattg taagtataaa cttgttgttt
     3361 cgattgttca atttttttta ttaatgcaga taatgcgagt gtagatgttt gttggttt
//
LOCUS       DAOSRP010000143         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_25443_length_3362_cov_3.695797, whole genome shotgun sequence.
ACCESSION   DAOSRP010000143 DAOSRP010000000
VERSION     DAOSRP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25443_length_3362_cov_3.695797"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="rsxC"
                     /locus_tag="PLJ82_07830"
     CDS             <1..983
                     /gene="rsxC"
                     /locus_tag="PLJ82_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOR41449.1"
                     /translation="TKREDIIQKIADAGIVGLGGACFPTHVKLSPPPNTKAELLLINA
                     VECEPYLTCDHQLMLEKAEEIIVGIQILMTVLQVNKAIIGIENNKKDAISLLQQITSS
                     YSTIEVCPLAMCYPQGGEKQLIEAITKKQVPSGQLPINIGIVVQNVATTYAIYEAVQK
                     NKPLIERVITVTGKKVAKPGNFLVRIGTPLRVPLDMAGGMPEDTGKIIGGGPMMGRAF
                     MNADVPVTKRSAGLLVMPTLESARKEMQNCIRCAKCVTACPMGLEPYLLMSQAEKSMW
                     KEMEETYITDCIECGCCLFSCPSNRPLLDYLRMGKSTVLEQIRNRVQKVN"
     gene            1002..1976
                     /locus_tag="PLJ82_07835"
     CDS             1002..1976
                     /locus_tag="PLJ82_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOR41450.1"
                     /translation="MMNKLIISPAPHIHSGDSISKNMYAVLFALVPAYLVGLYFFGIG
                     AFVVSITAVLSCIIFEFLIQKFIFKTTTTIHDGSAMLTGLLLAFNLPSNLPIWIIIIG
                     ALVAIGIGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTTWPKPIPFNTSYLDAETAA
                     TPLAMIKYNFGNLPEKMDLLIGNMGGSIGEVSAIALILGGIYLLIKRVITWHIPVSML
                     VTTFLFTGILHLINPSLYVSPDIHLLTGGLLLGAIFMATDYATSPMNTVAQLVYGIGI
                     GLITVTIRLFGAYPEGVSFAILIMNGVTPLLNLYVKPKRFGAPKTKKN"
     assembly_gap    2019..2028
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2029..2635
                     /locus_tag="PLJ82_07840"
     CDS             <2029..2635
                     /locus_tag="PLJ82_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOR41451.1"
                     /translation="NLPNMVMVLTAITLIASFLLASVYGVTKAPIELAKEKKKEVAIK
                     NVLPPFNRIENDTIEGIVVYKGYKDANWVGTAVESVSKNGFGGEIKIIVGFDAKGCIF
                     NYTVLEQKETPGLGTKMIDWFKTSKNKQSILGKNIAQEELKVSKDGGNIDAMTASTIS
                     SRAFLEAVNNAYSTISHYDTHTSATQVSDAATAATPWVENK"
     gene            2650..3237
                     /locus_tag="PLJ82_07845"
     CDS             2650..3237
                     /locus_tag="PLJ82_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOR41452.1"
                     /translation="MNAIKIVLNGILKENPVFVLLLGMCPTLGTTSSAINGLGMGIAT
                     MFVLIGSNTVISLIKNVIPDKIRIPAFVVVIAAFVSIIQMLMEAYLPELYASLGLFIP
                     LIVVNCIVLGRAEAFAAKNGVFHSALDGLGMGLGFSLALTLLGSTRELLGTGKIFNMF
                     IYPEHYGMLIFVLAPGAFIVLGFLIVFVTKLQTSK"
     gene            3245..>3362
                     /locus_tag="PLJ82_07850"
     CDS             3245..>3362
                     /locus_tag="PLJ82_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOR41453.1"
                     /translation="MSYIIIFIAAVFVNNIVLSQFLGICPFLGVSKKINTALG"
BASE COUNT         1091 a          516 c          649 g         1096 t
ORIGIN      
        1 caacgaaaag agaagatatt atacagaaaa tagcagatgc tggtattgtg ggtttaggtg
       61 gagcttgttt tcctacacat gttaagctat ctcctccccc caatacaaag gcagaattac
      121 tactaattaa tgcagtagaa tgtgaaccat atttaacttg cgaccatcaa ttaatgctcg
      181 aaaaagcaga agaaataatt gtaggtattc aaattttaat gactgtattg caagtaaaca
      241 aagccattat tggcatagaa aataacaaaa aagatgccat ttctctatta cagcaaatca
      301 caagttcata ttcaactatt gaagtttgcc ccttagcaat gtgttaccca cagggaggcg
      361 aaaagcaact aattgaggca atcacaaaaa aacaagtgcc aagtggtcaa ttacctatta
      421 atattggtat tgtcgttcag aatgtggcaa caacatatgc catttacgaa gcagttcaaa
      481 aaaacaaacc tttaatagaa cgtgtaataa cagttacggg gaaaaaagtt gcaaaaccag
      541 gtaatttttt agtacgtatt ggaactcctt tacgcgttcc attagatatg gctgggggaa
      601 tgcccgaaga tactggaaaa attataggtg gaggtccaat gatgggacga gcttttatga
      661 acgcagatgt acctgttacg aaacgtagtg ctggtttatt agtaatgcct acccttgagt
      721 ctgcccgtaa agaaatgcaa aattgcattc gttgtgccaa atgtgtaaca gcttgtccaa
      781 tgggtctaga gccctactta ttgatgtctc aggctgaaaa aagcatgtgg aaagaaatgg
      841 aagaaacgta cattactgat tgcattgaat gtggttgctg tttgttttct tgtcctagta
      901 atcgaccact attagactat ctacgtatgg ggaaaagcac tgttttggaa caaatacgta
      961 atcgcgttca aaaagttaac taattgaaat ctacacaaag tattatgaat aaactaatta
     1021 tttcgccagc tccacatata catagtggcg attctatttc taaaaatatg tatgcagtac
     1081 tatttgcatt agtacctgcc tatttagttg gtttgtattt ttttggaatt ggtgcttttg
     1141 ttgtcagtat tactgccgtt ttatcttgta ttatttttga atttttaatt caaaaattta
     1201 ttttcaaaac taccactact atccatgatg gttctgccat gcttacgggc ttattattag
     1261 cttttaacct cccatctaac ctacccatat ggattattat tattggagca ttagtagcga
     1321 taggtattgg taaaatgagt tttggaggat taggtaacaa tccatttaat ccagctttag
     1381 ttggtcgtgt atttttatta atatcttttc ctgtacaaat gacaacttgg cccaaaccaa
     1441 taccatttaa tacaagttat ttggatgctg agactgctgc tacacctttg gctatgatta
     1501 aatataactt tggtaattta ccagaaaaaa tggacttact cattggtaat atgggtggta
     1561 gtattggaga ggtatctgct atcgcattaa ttctaggagg aatctatttg ctgatcaaac
     1621 gtgttattac atggcatatc ccagtaagta tgttagttac tacatttttg tttacaggga
     1681 tattgcattt gattaatcct tctttatatg tatctcccga cattcacctg ttaactggag
     1741 gattgctatt gggagctatt tttatggcta ctgattatgc cacatcgcct atgaatacag
     1801 tagcacagtt ggtatacgga ataggtatcg gactgattac agtaacaata cgtttatttg
     1861 gagcctatcc agagggagta tcttttgcta tattgattat gaatggtgtt acaccattat
     1921 tgaatctata tgttaaaccc aaacgatttg gggcaccaaa aacgaaaaaa aactaaagta
     1981 ggtattcaaa ataacatact aaatgaaaaa attagaatnn nnnnnnnnaa atttgcctaa
     2041 tatggtcatg gttttgactg ctattacctt aatagcatca tttttgttgg caagtgttta
     2101 tggtgttact aaagcaccaa ttgagttggc taaagaaaaa aagaaagaag tcgcaattaa
     2161 aaatgtactg cctcctttta atcggataga aaatgacaca attgagggaa ttgttgtata
     2221 caaaggttat aaagatgcta actgggtagg aacagctgta gaaagtgtat caaaaaatgg
     2281 gtttggtgga gaaataaaaa taattgtagg ttttgatgca aagggatgta ttttcaatta
     2341 tacagtttta gaacaaaagg aaacgccagg actaggtact aaaatgatag attggtttaa
     2401 aacttcaaag aataagcaaa gtattcttgg taagaatata gctcaggaag agttaaaggt
     2461 ttcaaaagat ggtggaaata ttgatgcaat gacagcctca acgattagtt caagggcgtt
     2521 tttagaagcg gtgaataatg cctattccac catatcacat tacgacacac atactagtgc
     2581 gacacaagta tcagatgctg ctacagcagc tacaccatgg gtagaaaata aataaaaaca
     2641 ataggataca tgaatgcaat taaaatcgtt ctaaatggga tattaaaaga aaatcctgtt
     2701 tttgttttgt tactggggat gtgccccact ttaggaacta caagttctgc tattaatggg
     2761 ctcggaatgg gcatagctac tatgtttgtt cttattggtt caaatacggt aatttctctg
     2821 attaagaatg taatacctga taaaattcgc attcctgcat ttgttgttgt cattgctgca
     2881 tttgtaagta ttattcaaat gttaatggag gcttatttac cagaattata tgccagttta
     2941 ggcttgttta ttcctcttat tgttgtaaac tgtatcgtat taggtagagc cgaagctttt
     3001 gctgccaaaa atggcgtatt tcattcagcc ttagatggat tgggtatggg attggggttt
     3061 tcgttggcat taaccctatt aggatcaaca agagaattac taggaacagg taaaatattt
     3121 aatatgttta tttatccaga acattatgga atgctaattt ttgttttggc acctggagcg
     3181 tttattgttt tgggattctt aatcgtcttt gttacaaagc tacaaacatc aaaataaata
     3241 catcatgagt tatatcatta tttttatcgc agctgttttt gtaaacaaca ttgtattatc
     3301 acaattttta ggtatttgtc cgttcttggg ggtatctaaa aaaattaaca ctgcccttgg
     3361 aa
//
LOCUS       DAOSRP010000144         3344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_25655_length_3344_cov_4.401034, whole genome shotgun sequence.
ACCESSION   DAOSRP010000144 DAOSRP010000000
VERSION     DAOSRP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25655_length_3344_cov_4.401034"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1388)
                     /locus_tag="PLJ82_07855"
     CDS             complement(261..1388)
                     /locus_tag="PLJ82_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HOR41454.1"
                     /translation="MNYVERYTELNRLFSINNSTSVIYTPYATIHFASFESIPEMCHH
                     TSSNHIIDVDALISENMHFVYPIFMPHSTVRSGKAILLLHGLNERSWNKYLAWAEYLC
                     YTTNKPVILFPLAYHINRCPSSWSNPREIATIFHLRRQLNGNDRYISVANVVLSNRIS
                     EKPHRFYCSGRQSIADICSLISTIYKGVHPMLPDVSTVDIFAYSIGAFVAEIALLANP
                     LNLFDDTKLFLFCGGSVFSAMQGKSRGIMDTNAFDMLHTYYLQERNNHLKQFQKNDAI
                     DAAFETMISAQYNKQVREKFFLSMGNKIKGIALSKDVVIPYAGIVEALGSVCANNQIT
                     QLDFDYQYSHENPFPTNHIHQTNEVNEGFNQVFSYARDFFS"
     gene            1613..2470
                     /locus_tag="PLJ82_07860"
     CDS             1613..2470
                     /locus_tag="PLJ82_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOR41455.1"
                     /translation="MNNLIPSYLYEKTNLTRLVIFTAIFSLFFINLFQPFGSREWYPN
                     ISSMSYFFFSSLIILTGMLVVVLSRIIMYIHSKKHPILYWHYGVWILVEIVSMSLFYA
                     LFSMYVPKQGSVRAFEVILQNSIINTSLVLLLPYSILWLYFSWKEKNLQLLKLSQDET
                     GSDGQKKAILPFYDEKGELRISIAQENVLYIESSDNYITIHYLNKSKITGFLLRNSLK
                     WMDEHLSSDSPMVRCHRSYIVNLDRVKVLRKTKDGIFLELDALQTPDIPVSKTYYEKV
                     MKKFSHYSV"
     gene            complement(2745..>3344)
                     /locus_tag="PLJ82_07865"
     CDS             complement(2745..>3344)
                     /locus_tag="PLJ82_07865"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOR41456.1"
                     /translation="FFTNIGAWKASQYNKSSQYFTLAPTYRINDKLALTYQFISSFLQ
                     NAIGYVDKNNTNDSIFFGRRDISNIENIAQVKYSLSNNSLLNLRLRHYWSNVDHHAYY
                     LLQHDGTFQSTIREANYNTNVSALNADISYTWRFLPGSELSLVWKRYFYLYNQDVASS
                     YVKNLQNVLGYSQVNSVSLKLIYYIDYQTLKTAKKAIFL"
BASE COUNT         1172 a          516 c          551 g         1105 t
ORIGIN      
        1 ttaataccgc ttttctctca aagcgggtgc aaagataacg cctttattcc cgattttcca
       61 aataattaag aaactatttt taatctattt ttatctaaat tacttaacta cattaaaatc
      121 aatgggataa tctagtaact tttttttcta aaattagaga ttggatggaa taatagagaa
      181 ttgtagtggg agtataacca atcttaagca ggaattatac tgcaaaaaga gtaaaaaaag
      241 catgtatata tatgtgttaa tcaactaaaa aaatctcttg cgtaagaaaa aacctgatta
      301 aaaccctcat ttacttcgtt tgtttgatga atatgattag taggaaatgg attttcgtgt
      361 gaatactggt agtcaaaatc taattgtgta atttgattgt tagcacaaac actacctaat
      421 gcttccacaa tcccagcata tggtataacc acgtctttag ataacgctat cccttttatc
      481 ttattcccca ttgataaaaa gaatttttca cgcacttgtt tattatattg tgccgaaatc
      541 atggtttcaa aagcggcatc aatagcatcg tttttttgaa attgtttcag atggttattc
      601 cgttcttgta aataataagt atgtaacata tcaaaagcat tggtatccat aataccacga
      661 gatttacctt gcattgcact aaaaacagaa ccaccgcaaa ataagaataa cttggtatca
      721 tcaaataggt ttaacggatt ggcaagcaat gctatttctg caacaaacgc tccaattgaa
      781 tatgcaaaaa tatctaccgt agatacatct ggaagcattg gatgaacacc cttgtatatg
      841 gtgcttatta gcgaacaaat atcagcaata gattgcctcc cagaacaata aaaacgatga
      901 ggtttttcgg atatacgatt actcaataca acatttgcca cacttatgta cctatcattc
      961 ccattaagtt gacgccttaa atgaaaaata gttgctatct ctctaggatt tgaccaacta
     1021 ctagggcaac ggttgatatg ataggctaat ggaaataaaa ttactggttt gttggtggta
     1081 taacacaagt attctgccca tgcaagatat ttattccacg aacgctcgtt taacccatgc
     1141 agtagtaaaa ttgctttgcc tgatcgcaca gttgaatgtg gcataaatat agggtataca
     1201 aaatgcatat tttcactaat caaagcatcg acatcaataa tgtgattaga agaagtatga
     1261 tgacacatct ctggaatact ttcgaaagaa gcgaagtgta tagtagcata tggagtataa
     1321 ataaccgagg tactattatt tattgaaaat agtcggttta gttctgtata cctttcaacg
     1381 tagttcataa tggttatata taaatatgtt tattgtttat gacacaaaat aatgtgataa
     1441 aaattgagtc tcaaaatgaa agggaagaag agtattggaa tgggataaga gacgttaata
     1501 tgggataaaa attgacatag aggggtaaaa ataaaaagta attagataaa aaagtttgta
     1561 tgtatctgtt agaaaatgta cttttgcatc tacctctaaa tagaaaaaaa taatgaataa
     1621 tcttattcca tcttatttgt acgaaaaaac caatctcaca cggttggtga tttttactgc
     1681 tatattttcg ttgtttttta tcaacttatt tcagcccttt ggctcaagag agtggtatcc
     1741 aaatatctca agcatgtcgt atttcttttt ttctagcctt attattctta ctggtatgct
     1801 ggtggtagtt cttagtagga taataatgta tatacattct aaaaaacacc ctatccttta
     1861 ctggcattat ggagtttgga ttttagttga aattgtgagt atgtctttgt tttatgcatt
     1921 attttccatg tatgttccca aacaaggatc cgtaagagca tttgaagtaa tattacaaaa
     1981 ctctattatc aatactagtt tagttttgtt attgccttat tccatattat ggctgtattt
     2041 ttcgtggaaa gaaaaaaatt tgcaattact aaaactttcg caagatgaaa ctggtagcga
     2101 tgggcaaaaa aaagctattc ttccttttta tgatgaaaaa ggagagttgc ggatttcaat
     2161 agcacaagaa aatgttctgt acattgaatc atctgataat tacataacta ttcattatct
     2221 caataaatca aaaataacgg gtttccttct tcgtaattcg ttaaaatgga tggacgaaca
     2281 tctttcatct gactctccca tggtacgttg tcatcggtca tatattgtta atttagatag
     2341 agtaaaggtg ttgagaaaaa ccaaggatgg tatttttcta gaattagatg ccctgcaaac
     2401 gccagacata cctgtgtcta aaacgtatta cgaaaaggta atgaaaaaat tctcacatta
     2461 ttcagtataa taatgtaggt atatatgcca caaaaaaaga tgaaagcaac cctttacgaa
     2521 aattctgtga ttagcatctt tcatagaatc atttacacat aataaacaag tattacagga
     2581 agttggagaa aaagcctccc aaactttatc ccgtttttgg gaagatgttg taaaaaaagt
     2641 cgctgtacat tacgaacgta ttttagtgaa taaacaaaca cgtgtaaaaa ataagaattg
     2701 cttgaggcaa aaataagatc cttccattat attagagata ataatcacag aaaaattgct
     2761 ttctttgctg tttttagagt ttgataatcg atatagtaaa tcaattttag tgaaacacta
     2821 tttacttgag agtaacctaa tacgttttgc aaatttttga cataactact agcaacatct
     2881 tggttgtaca gataaaaata tcttttccaa accaatgata gttcgcttcc gggcaaaaaa
     2941 cgccacgtgt aacttatgtc agcatttaat gcactaacat tggtattata attggcctcc
     3001 ctgatagttg actgaaatgt gccatcgtgc tgtaaaagat aatatgcatg atggtctaca
     3061 tttgaccaat aatgacgtaa tcgaagattt agcaaactat tgtttgacaa agaatatttg
     3121 acttgtgcaa tattctcaat atttgaaata tctcttctgc caaaaaagat tgaatcattg
     3181 gtgttatttt tatcaacata ccctatagca ttttgcaaaa aactacttat aaactgatac
     3241 gtgagtgcaa gcttatcatt aatgcgatac gtaggagcca atgtaaaata ttgggaactt
     3301 ttattgtatt gagaggcttt ccaagcacct atatttgtaa aaaa
//
LOCUS       DAOSRP010000145         3267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_26474_length_3267_cov_5.231320, whole genome shotgun sequence.
ACCESSION   DAOSRP010000145 DAOSRP010000000
VERSION     DAOSRP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26474_length_3267_cov_5.231320"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1131)
                     /locus_tag="PLJ82_07870"
     CDS             complement(43..1131)
                     /locus_tag="PLJ82_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOR41457.1"
                     /translation="MKLKWLTNAFLLLLNSIVLVILLVINYVSVLSPDSVWSVLAPTN
                     ILFTAFVSINLLFVLWWIIHLKWYAIFSVLALLLCYTNISNTFIIWSEKPFPEENKPI
                     YSVVSYNIHHFKFFPKEKETKTGNHILDYLVELDADIICLQEFAYSDYEPFTLSDIKN
                     QLSDYPYNHIEILYKDRHISKGIATFSKHKITNKQHISIPAEYHGAISSDVLLDGKKI
                     RIINFHFESNRLTSKDKDIKRLTDRNKIPSFAKRLQSKLHTASRKRGKQAEALMTFAT
                     NSNMPTIICGDMNDVPSSYTYRTITQHYTDNFKQVGKGFGNTFHENFYRYRIDYTFTN
                     KQINPLKIKTHPYDISDHFPLQLDFQTP"
     gene            complement(1236..2030)
                     /locus_tag="PLJ82_07875"
     CDS             complement(1236..2030)
                     /locus_tag="PLJ82_07875"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR41458.1"
                     /translation="MWSQIKTNFKQGSYLTRLLYINLGMFVLVRLVLLIFTLFKHDIS
                     FAIRYIEMPAYIGNWITQPWGIITYMFLHVDFIHFIFNVLVLYWYGTIFLQFFNQKQL
                     VGVYILGGIGGAFLYVFGYSVFPYFQEVLKHTYLLGASASVMALIFTTVIYAPNYEVQ
                     LALIGRVKLKYIGLVLFAVDVLGVTSVNAGGSIAHIGGAITGILFASLYVRQRVDLTT
                     GISWLLDKLATLVQPKPKITVSYGRPKTDAEWNVEKKLKMQLLMKF"
     gene            complement(2061..2816)
                     /locus_tag="PLJ82_07880"
     CDS             complement(2061..2816)
                     /locus_tag="PLJ82_07880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOR41459.1"
                     /translation="MNNSFLGGIPPVVKNLLIINILFWLASLTLPGVIGIDVVDWLGL
                     HYWQSDKFVVSQLVTYMFLHDTSSLFHLFFNMFALYMFGKNIEYIWGTKKFLLFYFVT
                     GIGAAIVQQITWSFDFVPIANAFGEYVNNRDISVLMPYLNVNESTVLSTTEVLQLKNM
                     VLNQPITVGASGAVFGILLAFAMLFPDARIMLLFLPVPIKARYFVIFYGAMELFLGVS
                     NFQGDSIAHFAHLGGMLFGLVLILLWRKKGTLY"
     gene            3023..>3267
                     /locus_tag="PLJ82_07885"
     CDS             3023..>3267
                     /locus_tag="PLJ82_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR41460.1"
                     /translation="MNKAELISAIADNSGLSKVDSKKALDGFIAAVSNALKSGDKVAL
                     VGFGSFDVAERSARTGVNPATGAKIKIDAKKVVKFKAG"
BASE COUNT         1154 a          550 c          528 g         1035 t
ORIGIN      
        1 gacaaattag ggtaatttca ggatgaaaag atactgtaaa agttaaggtg tctgaaagtc
       61 tagttgtaag gggaaatggt cagaaatatc ataaggatga gtttttattt ttaatgggtt
      121 gatttgctta tttgtaaaag tataatcaat acgatatcgg taaaagtttt cgtgaaacgt
      181 attgccaaaa cctttgccta cttgtttaaa attgtctgta taatgttgag taatcgttct
      241 atatgtatac gaagaaggga catcattcat atctccacat ataatagtag gcatgttaga
      301 gtttgttgcg aatgtcatta atgcttctgc ttgtttgcct cttttgcgtg acgcagtatg
      361 aagtttcgat tgcaatcttt tagcaaacga tggtatttta tttcgatcag ttaagcgttt
      421 tatatctttg tctttgctgg ttaatctgtt cgattcaaag tgaaaattaa taatgcggat
      481 ttttttgcca tctagcagaa catcagagga aatggctcca tgatattctg cagggataga
      541 aatgtgttgt ttattcgtta ttttgtgttt tgaaaaagtg gcaatacctt ttgagatatg
      601 tctgtcttta tataatattt ctatatgatt gtatggataa tcagacagtt gattctttat
      661 gtcagataat gtaaacggct cgtaatcact atatgcaaat tcttgcaagc agattatatc
      721 ggcatcaagt tctaccaaat aatccagaat atgattgcct gttttcgttt ctttttcttt
      781 agggaaaaat ttaaaatggt gtatattgta cgaaaccacg gagtatatag gtttattttc
      841 ctctggaaaa ggcttttcag accaaataat aaacgtatta ctaatatttg tataacaaag
      901 gagtagggca agaacagaaa aaatagcata ccacttcaaa tggataatcc accatagtac
      961 aaacaataaa ttgatactaa caaaggctgt gaaaagaatg ttagtaggag ccaatactga
     1021 ccataccgag tcgggtgaca atacagaaac ataattgatt actaacaaaa tcactaatac
     1081 aatgctattg agcaataaaa gaaatgcatt tgtcaaccac ttgagtttca tgttggtcgg
     1141 tattttttag ttattgtttt ttttattgct aatgtcaaac agcatttttt tttgttcagc
     1201 actcaaatta tcgtaacctg atttttttat ttgttctaaa atttcatcaa gagctgcatt
     1261 tttagctttt tttctacatt ccattcggca tctgttttcg gtctgccata gcttacggta
     1321 atttttggtt ttggttgcac taaggtcgct agtttatcaa gtaaccaact aatgcccgta
     1381 gttaaatcga ctcgttggcg aacatataaa ctagcaaaca atatgcccgt aatggcacct
     1441 ccaatgtgag caatactccc tccagcattt actgaggtta cacctaatac atctactgca
     1501 aataaaacta aaccaatgta tttcaattta acacgcccaa ttaaagcaag ttgcacttca
     1561 taattaggag catatatcac cgtagtaaaa atcagagcca tcaccgatgc cgaagcacct
     1621 agcaaatacg tgtgtttcaa tacttcttgg aaataaggaa acacagaata cccaaaaaca
     1681 tataggaatg caccaccaat acctcccaat atatacacac ctactaactg tttttggttg
     1741 aaaaattgca aaaaaatggt gccataccag tacaatacca aaacattaaa tataaagtga
     1801 ataaaatcta catgtaaaaa catataggta atgatgcccc atggttgtgt gatccaattt
     1861 cctatatatg caggcatttc tatatatcgt atagcaaaac tgatatcatg tttgaacaag
     1921 gtaaaaatca ataataccaa acgaaccaat acaaacatgc ccaagttgat atacaacaag
     1981 cgtgtaagat aagagccttg tttaaaatta gtttttattt gagaccacat agtcattttt
     2041 tttctttagt gtttaaacca ttagtataag gtgccttttt tacgccaaag cagaattaac
     2101 actaagccga ataacatacc ccccaaatga gcaaaatgag ctatactatc tccttgaaaa
     2161 ttacttacac ctaaaaacaa ttccatagca ccataaaaaa taacaaagta tcttgcctta
     2221 atgggcacag gtaaaaataa gagcataata cgtgcatcag gaaataacat agcaaacgca
     2281 agcaatatac caaacactgc acctgaagct ccaactgtaa taggctggtt cagtaccata
     2341 ttttttagtt gcaacacttc agtggttgac aatacggtag attcatttac atttaagtaa
     2401 ggcattaata cactaatatc acgattattt acatattcgc caaaggcatt agcaatagga
     2461 acaaaatcaa acgaccatgt tatttgttgc acaatggcag ctccaatacc tgttacaaaa
     2521 taaaaaagta gaaacttttt tgttccccaa atatattcaa tgttttttcc aaacatatac
     2581 agggcaaaca tattgaaaaa taaatgaaac aacgagctag tatcatgtag aaacatatac
     2641 gtaactagct gagataccac aaacttatct gattgccaat aatgcaagcc taaccagtca
     2701 acgacatcaa tacctattac accaggtagg gtgagagaag ccaaccagaa aagaatattg
     2761 ataataagta aattttttac aacaggaggt attcctccta gaaaagaatt attcataact
     2821 tcctacttaa ttgatgtaca aaagtagtta tttttttatg gaaaaaccct gaaaaatcca
     2881 cagtttgggg aaattttcac caaaaagtat attttttttt aaaaaagtat tgcaattaat
     2941 taattttcaa ttacatttgc caaagtatga aattttattt ttaattaatt tattttttta
     3001 accactctaa atcaaaaaca ttatgaacaa agcagaactt attagtgcaa ttgctgacaa
     3061 ttcaggtctt agcaaagttg attcaaaaaa agcgcttgat ggtttcatcg cagcagtatc
     3121 taacgcatta aaatccggag ataaagttgc ccttgttggt ttcggttctt tcgacgtagc
     3181 tgaaagaagt gctcgtacag gtgttaatcc agctacaggt gccaaaatca aaattgatgc
     3241 taaaaaagtt gttaaattta aagcagg
//
LOCUS       DAOSRP010000146         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_26581_length_3258_cov_4.797690, whole genome shotgun sequence.
ACCESSION   DAOSRP010000146 DAOSRP010000000
VERSION     DAOSRP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26581_length_3258_cov_4.797690"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="dnaX"
                     /locus_tag="PLJ82_07890"
     CDS             complement(<1..733)
                     /gene="dnaX"
                     /locus_tag="PLJ82_07890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR41461.1"
                     /translation="MDNYIVSARKYRPITFDSVIGQHALVTTLKNAIKNKQLAHAYLF
                     CGPRGVGKTTTARIFAKTINCLQPLPNHEACNQCESCMAFNEQRSFNIHESDAASNNS
                     VDDIRSLIDQVRIPPQIGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPAHAIFILATTE
                     KHKILPTILSRCQIYDFNRISVQDTVTHLQHIASLEGITAEPEALHVIAHKSDGGMRD
                     ALSTFDQVVSFCGNTITYKHVIENLN"
     gene            1005..1301
                     /locus_tag="PLJ82_07895"
     CDS             1005..1301
                     /locus_tag="PLJ82_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOR41462.1"
                     /translation="MKKIVDFFITYILNKYVIASAVFVFVMFTNEDYNIKTRYANAQK
                     IKKLEAEIRYYQEETKKNKEKLDLLQSDKENLERFAREQFIMKKDSEDVFVVVD"
     gene            1312..1626
                     /locus_tag="PLJ82_07900"
     CDS             1312..1626
                     /locus_tag="PLJ82_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41463.1"
                     /translation="MKLLYIISFLAVLVATILYMFFPQVAPFVFSVGVVGIIVFRLRT
                     LYAGSDFRVKRLYIMQNLATLLYIAVAYFMFIQSDIWVFILLVAALVETMVVLRMPNK
                     RE"
     gene            complement(1910..2413)
                     /locus_tag="PLJ82_07905"
     CDS             complement(1910..2413)
                     /locus_tag="PLJ82_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOR41464.1"
                     /translation="MILIKKLHPDSVVPTSKNYIVGVGAIVLNKGNLLVIKDKLSVGY
                     KLPGGYIDKNESIKKALKREVYEETGITIEFESIVNIGHFIHGQFGESNLYIVCTAKA
                     LSQKIKIHDSAEIAEAKWIDPVEFLHSDNVTMYNKSVVEAIINNKGVKLLEQKITLKV
                     PNGEVFF"
BASE COUNT         1018 a          496 c          589 g         1155 t
ORIGIN      
        1 tatttaggtt ttcaatcacg tgtttgtatg taattgtgtt tccacaaaaa ctgacaactt
       61 gatcaaaagt agaaagtgca tcacgcatac caccgtccga tttgtgtgca ataacgtgta
      121 aagcttcagg ctcggcagta atcccttcta aagaggctat gtgttgcaaa tgagttacgg
      181 tatcttgtac gctaatgcga ttaaaatcat aaatctgaca acgagataaa atcgttggta
      241 aaattttgtg tttttctgtc gtagccaaga taaatatggc atgagcagga ggttcttcta
      301 aggttttcag aaaagcgttg aacgcacttt gcgaaagcat gtgtacctcg tcgatgatat
      361 atacactgta tttgccaatt tgtggtggaa tgcgtacttg gtctattaat gaccgaatat
      421 catctaccga gttattagag gcagcatccg attcgtggat attaaacgag cgttgttcgt
      481 taaatgccat acacgattcg cattggttgc aagcctcgtg gttgggtagg ggttgcaagc
      541 agttaatcgt tttggcaaaa attcgagctg ttgttgtttt tcctacacct ctaggtccac
      601 aaaacaaata agcatgagcc aattgcttgt ttttaatagc atttttgagt gtagtaacca
      661 atgcatgctg accaataacc gaatcgaatg ttatggggcg gtattttcgt gccgaaacaa
      721 tgtaattatc catttgcttt tttttccttt gcacaaagat aaaaaaatgt tcataaatag
      781 aacgtaaaat ttagcttttt aaagaaaaaa aactacattt gtaggttgat attaaacttc
      841 agtctgctat aattggttta cctgttgtct tgttgtgatt gggttctttt agaggaatcc
      901 tttgtttgca gggataaatg aaagtagccc attagtggga acagataaag aagcatatat
      961 gtatctcaat tttatttaat atagttgcca tttaacaatc tagtatgaaa aaaatagtag
     1021 atttttttat cacatatatt cttaacaaat acgttatagc atctgctgtt tttgtttttg
     1081 tgatgtttac caacgaagat tataatatca aaacaaggta tgcgaatgca caaaaaatca
     1141 aaaaactaga ggcagaaata cgctattacc aagaagaaac aaagaaaaat aaagaaaaac
     1201 tagatttatt gcagtcagat aaagaaaatt tggaacgatt tgcccgtgag caatttataa
     1261 tgaagaaaga tagtgaagat gtttttgtgg tggtagatta aaatttgtag tatgaagttg
     1321 ttgtatatta tttctttttt agcagtactt gtagctacta ttttgtatat gttttttcca
     1381 caagtagctc catttgtttt ttcggtagga gttgtaggta ttatcgtttt tcgtttgcga
     1441 acgctctatg ctggatccga ttttagagta aaacgattat atataatgca aaatttagca
     1501 accttactat atatagcggt tgcgtatttt atgtttattc aaagtgatat ttgggtattc
     1561 atattgctcg tagcagcctt ggtagaaacg atggttgtat tgcgtatgcc caataagagg
     1621 gagtaagagc agggtagaca agtttgcgta tgcattgatt tagtaaacag tgtgcaataa
     1681 aaactacccc gttttttaca atagattgtt acaaaacagt ggagtacggg aaagttaaca
     1741 tgtatacgcg ttacagcata acgatataag taaattttat atagtgtaat tgatgggagt
     1801 acttaccaca aggtttcgtt aagggatttt ttttgtttta tcgatgtttt gcaaaacgta
     1861 cagattcatg gcaattaacc cgatgcttgc gttgtcaatc agattctttt taaaaaaaca
     1921 cttctccatt aggtactttt aatgtaattt tttgttctaa aagcttgact cctttgttat
     1981 tgatgatagc ttcaacaacg cttttgttat acatagtaac gttgtcggaa tgtaaaaatt
     2041 ctacagggtc aatccatttt gcttcggcta tttcagccga atcgtgtatc ttaatttttt
     2101 gggataaagc ctttgcagta cacacaatat aaagattaga ttctccaaat tgtccgtgta
     2161 taaaatgtcc aatattaaca attgattcaa attcaatagt aattcctgtt tcttcgtaaa
     2221 cttctctttt tagagccttt tttattgatt catttttgtc aatatagcca cctggaagtt
     2281 tatatccaac cgataattta tctttaatca ctaataaatt ccctttattc aacacaatag
     2341 ccccaacacc tacgatgtag tttttagatg tagggacaac agagtcagga tgtaattttt
     2401 tgataagtat caaatttttc tcatcgcaat gatgaaattc aaaatctaaa tctgtaagta
     2461 ttgggataaa atccgatttc tcaattggga tactaatcta cagaagtttt ttatttgtta
     2521 aagattgaat tagtcgtact atttcttttt tacattcatc tttgtttggt ggaagtgttg
     2581 cattgttaac tattactcca tcatatgcat tgttacttat ttctatcata gatgattatt
     2641 ttttttcgat atgattgcta atgctatcta cattcataga atcaattctt tctactatag
     2701 ttgaaagcga acataaaaag aatactgacc tgtatcttgt gtatcgtttc ctactgtttg
     2761 gatttttgct atatgttcta actaacccat tttcagtttt tttacatcac ttttttatcc
     2821 ccaaaaaata gaggaaaaat tatgaggtac tttggcaaag atatacattt gtatacagat
     2881 aaaagttctt atttgcttta aaatatctca gaaacgcttt tagctaacta tgtgaccaaa
     2941 ttgtgtgaaa aatagaaaga tgatgattct ttacaaataa aaataggtgt ttttgtttcc
     3001 attttacact tttttgtgta aacctatttc aggacaagac actatctatc gttacaaacg
     3061 acaaaccata aactcctcgt tgaaaacgag gggtaaggga tttcttatgt tttttttgtt
     3121 tagttcgaat aaggaaagct ggagctttcc ttttaacttc gacatagcga gacgctatgc
     3181 cgaacacgca gattgtcaaa attcacacta gggcgaacag gggggaggag tagcttgtag
     3241 ccccatatta ctaactac
//
LOCUS       DAOSRP010000147         3219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_27008_length_3219_cov_4.521176, whole genome shotgun sequence.
ACCESSION   DAOSRP010000147 DAOSRP010000000
VERSION     DAOSRP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27008_length_3219_cov_4.521176"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2054
                     /gene="argS"
                     /locus_tag="PLJ82_07910"
     CDS             261..2054
                     /gene="argS"
                     /locus_tag="PLJ82_07910"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOR41465.1"
                     /translation="MNLTQQVENFILQAIPQLYHQEIDISLVQIQKTKKDFDGELTVV
                     TFPFVKLARKSPEAIGVELGEWLVANAPIIKSFNVIKGFLNLSINPRYWLQMLQMITQ
                     DPYYGSKLPKPDAPVMMIEYSSPNTNKPLHLGHIRNNLLGYSIAEIQKANGWNVVKTN
                     IVNDRGIHICKSMLAWQKFGNGETPESSGMKGDHLVGKYYVIFDKEYKKQVADLLQQG
                     MSEDEAKKKAPLMIEAQAMLRKWENDDVTVRQLWEMMNKWVYAGFDQTYKQLGVDFDK
                     IYYESETYLEGKNKVEEGLSKGVFYKKEDGSVWADLTQDGLDEKLLLRADGTSVYMTQ
                     DIGTAKLRYDDYPIQKMVYVVGNEQNYHFQVLALLLDKVGFSWGKDLVHFSYGMVELP
                     EGKMKSREGTVVDADDLMQEMVQTALDTANELGKLDGLSVDEVQSIANMVGLGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTHARIQSVLRKAIDQGISISGEVQLQQD
                     LAEKEISLLRLLADFPAVIEQAGNDFSPALIANYAYDLVKEYNQFYHDFSILKETNVA
                     VRHFRLVLSREIARVIKSSMRLLGIDVPDRM"
     gene            2073..3218
                     /locus_tag="PLJ82_07915"
     CDS             2073..3218
                     /locus_tag="PLJ82_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41466.1"
                     /translation="MKKFNSFVAIGLIALLATTACNTAQKAYRKGDYYKATIEAIARL
                     RSKPDDSKAQTVLLNAYPMAQKTALREIENANVRDDLDKYDIMVYQYSRLNNIANQIY
                     ACPKAFELIPRPAEYQAEISEAKKKAAELLYNLGETALSYRTLEQSRIAYNHFNRANE
                     YVYGYKDVFNKIEESLYYATLRVLVERPITAGKYQLSAEFFSNNLIAEMTRQSQHKFI
                     RFYTIEEAQSLKMNNPHQFLIFDFTDYTIGNVRETINTQELKRDSVVVGKVNVDGKSY
                     NAYNTVYAKFTTNRREILSAGVLSVRIIDAPTNRILQHQNFVGEYVWYTEWSSYSGDE
                     RALSDKQKRMTQLQPQLPPPHQDLFIEFTKPIYSQVLTFVRSAYRNY"
BASE COUNT         1129 a          469 c          640 g          981 t
ORIGIN      
        1 ggcattagcg gtggcagttg ctgcttgggg taacaaaatt gatgctaaaa aagctgttaa
       61 atttaaagca ggtgctgaat tggctgactt agttaaatag gatagcaaat agaaaacact
      121 aaatattcta aaaaaatggg gtttcaaaag tattgagctc catttttttt atttactact
      181 attttcttcc caaaaaaaat agtatttttg ttcagtttaa gtacttatat aattccgtat
      241 cattttagct atatatatcc atgaatctta cccaacaagt agaaaatttt atattacaag
      301 caattcctca actttatcat caagaaattg atatttcttt ggtgcaaata caaaaaacaa
      361 aaaaagattt tgatggagaa cttacggtgg taacgttccc ttttgttaaa ttagcacgta
      421 aatcccccga agctattggg gtagagttag gagaatggtt agtagcaaat gctcctataa
      481 taaaaagttt taatgtgatt aaaggctttt taaacttgag tataaaccca cgctattggt
      541 tgcaaatgct ccaaatgatt acacaagatc catattatgg aagtaaattg ccaaagccag
      601 atgctcctgt aatgatgatt gaatattcgt cgcccaatac aaacaaacct ctccatttag
      661 gtcatatacg caataatcta ttaggatata gtattgccga aatccaaaaa gcgaatggtt
      721 ggaacgtagt taaaaccaat attgtaaatg atagagggat tcatatttgt aaatccatgt
      781 tggcttggca aaaatttggt aatggtgaaa cgccagagtc ttctgggatg aagggcgacc
      841 atttagtcgg aaaatattat gtaatttttg acaaagagta taaaaagcaa gttgccgatt
      901 tgttgcaaca aggcatgtct gaagatgaag ctaaaaagaa agctccatta atgatagaag
      961 cccaagcaat gttgagaaaa tgggaaaatg atgatgtaac agtacgtcaa ctttgggaaa
     1021 tgatgaataa atgggtgtat gcaggttttg accaaacata caaacaactc ggtgttgatt
     1081 ttgataaaat atactacgaa tctgaaacgt atcttgaggg aaaaaataaa gtagaagaag
     1141 gtttatcaaa aggcgttttt tataaaaaag aagatggttc ggtgtgggcg gatttaacac
     1201 aagacgggct agacgaaaaa ttgctattgc gtgcagatgg cacatccgta tatatgaccc
     1261 aagatattgg cacagctaaa ttgcgttacg acgattatcc cattcaaaaa atggtgtatg
     1321 tggttgggaa tgaacaaaat tatcattttc aggtattagc acttttgttg gataaagtgg
     1381 gcttttcgtg gggtaaagat ttagtgcact tttcgtatgg tatggtagaa ctgcccgaag
     1441 ggaaaatgaa aagtcgtgaa ggtactgttg tagatgctga tgatttgatg caagaaatgg
     1501 tacaaacagc attagacaca gctaatgaac tagggaaatt agatggtttg tccgtggatg
     1561 aagtgcaatc catagccaat atggtaggtt taggtgcgtt aaagtatttt atactaaaag
     1621 ttgaccctcg caaaaatatg acgtttaacc caaaagaatc aattgatttt aatggaaata
     1681 cgggaccatt tattcagtat acacatgctc gtattcaatc cgtattgcgt aaagctatag
     1741 atcaaggtat ttctatttcg ggagaagttc aattacaaca agatttagcc gaaaaagaaa
     1801 ttagtttatt gcgtctattg gctgatttcc ctgctgtaat agaacaagca ggaaatgatt
     1861 ttagtcctgc attgattgca aattatgcgt atgatttggt aaaagaatat aaccagtttt
     1921 accatgattt ttcaatttta aaagaaacaa atgtagctgt tcgtcatttt cgcttggtat
     1981 tatcacgtga aatagcacgt gttataaaat cgagtatgag gctactaggt attgatgtgc
     2041 cagatagaat gtaacactta aaatgaatgc atatgaaaaa atttaactca tttgtcgcaa
     2101 ttggtttgat agcattactt gctactactg cttgtaatac ggctcaaaaa gcatacagaa
     2161 aaggagatta ttacaaggca acgatagagg caattgctcg tttgcggagt aaacccgatg
     2221 atagtaaggc acaaacagta ttgcttaatg cctatccgat ggcacagaaa acagcattgc
     2281 gtgaaattga aaatgccaat gtacgtgatg acttggataa atatgatatt atggtatatc
     2341 agtattcacg tcttaataat atcgcaaatc agatttatgc atgtcctaaa gcatttgagc
     2401 ttattcctcg tcctgctgaa tatcaggctg aaatttcgga agcaaaaaag aaagcagctg
     2461 aattattata caatttaggt gaaacagctt tgtcgtatcg tacactagaa caatctcgca
     2521 ttgcatataa ccatttcaat cgagctaacg aatatgtata tggttataaa gatgttttta
     2581 ataaaataga agagtcattg tattatgcaa cattacgagt attggtagaa agaccaatta
     2641 ctgcagggaa ataccagctt tcggcagaat tttttagtaa taatttgatt gcagaaatga
     2701 cgcgtcaaag tcaacataag tttattcgtt tttatactat cgaagaggct caaagtttga
     2761 aaatgaataa tccgcatcaa tttttaattt ttgattttac tgattatacg attggtaatg
     2821 tgcgtgaaac tatcaataca caagaactaa aacgagatag tgtggtagta ggaaaagtaa
     2881 atgtagatgg gaaaagttat aacgcatata ataccgtata tgcaaaattt actaccaata
     2941 gaagagaaat tttatcagct ggagtgttga gtgttcgtat cattgatgca ccaactaatc
     3001 gtattttgca acatcaaaat tttgttggag aatatgtatg gtataccgaa tggtcaagtt
     3061 atagtgggga tgaaagagct ctttcggata aacaaaaacg tatgacacag ttgcaaccac
     3121 aactccctcc acctcatcaa gatttgttta tagaatttac aaaaccaatt tattctcagg
     3181 tgcttacttt tgtgcgttcg gcttacagaa attattaat
//
LOCUS       DAOSRP010000148         3136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_27992_length_3136_cov_3.487179, whole genome shotgun sequence.
ACCESSION   DAOSRP010000148 DAOSRP010000000
VERSION     DAOSRP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27992_length_3136_cov_3.487179"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLJ82_07920"
     CDS             complement(<1..733)
                     /locus_tag="PLJ82_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR41467.1"
                     /translation="MSLFVKIIVDNHSQQDNFLTEHGICVYVETLTHKCLVDVGASDK
                     FIRNAEQMGIDIAQIDYLFLSHGHVDHVGGLPFFLKKNAKAKIIFSDKLFKQQYFSKR
                     HSTRSISLAYTFTEEELNRFMPIQQTAHIEADIKVIAQIQQNYPLPQGNRFLYTQAFD
                     DNEFVQDDFSHEIVVCFGSTSLLVCTGCAHNGLLNMLHTVQNQTSLPIHTVLGGFHLL
                     DADGTYAYETPKEITDIALTLKQTYP"
     gene            complement(730..1140)
                     /locus_tag="PLJ82_07925"
     CDS             complement(730..1140)
                     /locus_tag="PLJ82_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOR41468.1"
                     /translation="MPRPQQHRTLLTPPKMKGFKPFGIPTKSLSSVVLLYDEYESIRL
                     LDYEGLTQEQAAERMGVSRPTLTRIYGKARQTMAQAMVEGKMILIEGGNVSFNSQWYR
                     CKKCYKLIQGKENHLHCDNCPCYGDDELEKIGQE"
     gene            complement(1143..1502)
                     /locus_tag="PLJ82_07930"
     CDS             complement(1143..1502)
                     /locus_tag="PLJ82_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOR41469.1"
                     /translation="MKKTLVIGASENPERYAYKAITALVAHGHEVKALGLKKGNVAGI
                     EFDTEQLPYQGIDTVSLYVGPQNQPSYYEFIEKLQPKRVLFNPGTENSEFQARLIAKG
                     IEVEEACTLVLLSTNQY"
     gene            complement(1613..2353)
                     /locus_tag="PLJ82_07935"
     CDS             complement(1613..2353)
                     /locus_tag="PLJ82_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOR41470.1"
                     /translation="MIHSILIAILIGNLGSVLVVAFLLRFDMDVLLKWLKHVNAFAGG
                     TLLGAAFLGMLPKSVALGGPEMSYIVLVGIFFFFVLEKFLLWRICVDENCDRHSQTGA
                     RMLMVGDSFHNFLDGVVIAAAFIHSTSFGLIVTFSVFAHEIPQEIADFGVMIKSGFSK
                     KKAIWYNVISGLTALIGGFLSWYFGSIIQGLLPYVLAFSAAGFMYVSLADLIPELHKK
                     LHFKESVHQIVFLLLGILFIVFSIILKP"
     gene            complement(2403..>3136)
                     /locus_tag="PLJ82_07940"
     CDS             complement(2403..>3136)
                     /locus_tag="PLJ82_07940"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOR41471.1"
                     /translation="VGKSTIASALAILLSQQGHRVGLLDADLFGPSVPLLFGLQDEKL
                     QVKQVHDKQFLVPFTKNGLKLMSIGFLVEPHQAAVWRGPRVSAVVSQLLNDTDWGELD
                     YLLIDTPPGTGDIHITLLQNFSLSGVVIVTTPQEMSLVDVKKSINLFQSDKLSIPILG
                     IVENMSWFTPIKHPDEKYFLFGKGGGEAIAKEFHVPLLSQIPMDESICTNCDEGRLEK
                     MLTSKGIAQAFDVLIQQMLHRIING"
BASE COUNT         1022 a          640 c          523 g          951 t
ORIGIN      
        1 agggatatgt ttgttttaat gtaagagcaa tatcggttat ttcttttggt gtttcgtaag
       61 catacgttcc atcggcatct aacagatgaa atcctcctaa cacagtatgt atgggcaacg
      121 atgtttgatt ttgtactgta tgcaacatat tcagcaaacc attgtgggca catcctgtac
      181 acacaagcaa cgaagttgac ccaaaacaca ccactatctc gtgcgagaaa tcgtcctgta
      241 caaattcatt atcatcaaag gcttgagtat ataaaaaacg attcccttgt ggcaatgggt
      301 agttctgttg tatctgtgca attactttta tatccgcctc aatgtgagca gtttgttgaa
      361 ttggcataaa acgattcagc tcctcttcgg taaaagtata tgctagacta atagagcgtg
      421 tggaatgacg tttagaaaaa tattgttgct taaagagctt gtcagaaaag atgatctttg
      481 ctttcgcgtt ctttttcagg aaaaacggaa gaccaccaac atgatcgaca tgtccatgag
      541 aaagaaacag ataatcaatt tgagcgatat caatgcccat ttgttcggca ttgcgaataa
      601 acttatcaga ggcaccaaca tcgactaaac atttgtgtgt aagcgtttct acatacacac
      661 agatgccatg ctccgtaaga aagttatcct gttggctatg gttatccact attattttta
      721 caaacagact cattcttgcc caattttttc taattcgtcg tcgccataac atggacaatt
      781 atcgcaatgc aaatgatttt ctttgccttg tatgagtttg tagcattttt tacatcgata
      841 ccattgcgaa ttaaaagaaa cattccctcc ctcaatcaaa atcatcttac cctctaccat
      901 agcttgtgcc atagtttgac gagcttttcc gtaaatacgc gttaatgtag gtctagaaac
      961 ccccattctt tctgccgctt gttcttgggt gagtccttcg taatccaaca aacgaataga
     1021 ttcgtattcg tcgtacaaca acaccacaga agacaatgat ttagtgggga taccaaaggg
     1081 tttaaacccc ttcatttttg gcggggtcaa taaggttcta tgttgttgtg gtcgtggcat
     1141 acttaatact gattggttga aagcaatacc aatgtacaag cttcttctac ttcaattcct
     1201 ttggctatta atcgtgcttg aaattcggaa ttttcggtac ctggattaaa caatacgcgt
     1261 tttggttgaa gtttttcaat aaattcgtaa taacttggtt ggttttgtgg accaacatac
     1321 aagcttactg tatctatgcc ttgataaggt agctgctcgg tatcgaactc aataccagct
     1381 acgtttcctt tttttagtcc taaggctttt acctcatgac catgtgctac taaagccgta
     1441 atggctttgt atgcgtatct ttctggattt tctgatgctc caattacgag tgtttttttc
     1501 atattactta tttttatatt ttacaattaa ggagtacaaa attacaactt tattgcgaac
     1561 atatgttcac tgaacacgaa tatttgaaat aaaaaaactt aaaaaagtga tgctatggtt
     1621 ttaagataat agaaaatact ataaacaaaa taccgagtag caaaaacact atttgatgaa
     1681 ccgattcttt gaaatgcaac tttttatgaa gttcagggat taaatcggct aacgatacat
     1741 acataaaacc agctgccgaa aaagccaaca cgtagggcaa taacccttga ataattgacc
     1801 caaaatacca cgataaaaaa cctcctatta atgcagttaa cccagaaata acattgtacc
     1861 aaatagcttt tttcttacta aaaccagact taatcattac cccaaaatca gctatttctt
     1921 gaggaatttc gtgtgcaaaa acagaaaacg taacaattaa cccaaatgaa gtagagtgta
     1981 taaaagcagc agctattacc acgccatcta aaaagttatg aaacgaatcg ccaaccatta
     2041 acatacgtgc acccgtttgc gaatgtctat cacaattttc gtcaacacaa atacgccata
     2101 gtaaaaattt ttctaacaca aagaagaaaa aaatgcctac caacacaata taggacattt
     2161 ctggtccgcc taaggcaaca gatttgggca acataccaag aaaggcagcc ccaagcaatg
     2221 tccctccagc aaaagcattt acatgtttta gccatttcag caacacatcc atgtcaaaac
     2281 gaagcaaaaa agcgacgaca agcacacttc ccaagttgcc aattaaaatg gcaattaata
     2341 tactatgtat cataattaat tcgatgtatt acagattatt atacatttag caatagcatc
     2401 atttatccgt tgataatacg atggagcatc tgttgtatca acacatcaaa agcttgtgct
     2461 attcctttac ttgtaagcat tttttcgagg cgaccttcat cacaattcgt acaaatactt
     2521 tcatccattg gaatttgcga gagcaatgga acatgaaatt cttttgcaat cgcttctcct
     2581 cccccttttc caaataaaaa atacttctca tcaggatgct taatcggcgt aaaccaagac
     2641 atattttcta caattccgag tatcggtatc gacaacttat ctgattgaaa taaattaatt
     2701 gattttttaa catcaaccaa cgacatttct tgtggtgtag taacgataac gacaccagac
     2761 aatgaaaagt tttgtaacaa ggtaatatga atatcccctg taccaggagg tgtatcaata
     2821 agtaaataat ctaattcgcc ccagtcagta tcgtttagca actgagacac cacagccgaa
     2881 acccttggtc ctcgccaaac tgcagcttga tgtggttcca ctaaaaaacc aattgacatt
     2941 agttttaatc catttttagt aaacggaacg agaaactgtt tatcgtgaac ttgcttaact
     3001 tgtaattttt catcttgtaa tccaaaaagc aatggaactg atggtccaaa caaatcggca
     3061 tctaataagc cgacacgatg cccctgttgg cttaacagaa tagctaatgc cgatgcaatg
     3121 gtagattttc ccacgc
//
LOCUS       DAOSRP010000149         3055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_29006_length_3055_cov_5.576333, whole genome shotgun sequence.
ACCESSION   DAOSRP010000149 DAOSRP010000000
VERSION     DAOSRP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29006_length_3055_cov_5.576333"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1166)
                     /locus_tag="PLJ82_07945"
     CDS             complement(21..1166)
                     /locus_tag="PLJ82_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR41472.1"
                     /translation="MKNIYFVIIISVISVIYLLLNAYVYHRANVALPNKMARNTFTVL
                     FWLCSVSFLIGQFLERTPWVNLTTVISGIGSVWLALFFYALLFVLVIDIFALLHRLTS
                     LLPVSFLEIATPFRLFVVVAVSTISLVTAGYINARYPTVNEYEVRINKQVAGKKQLTV
                     ALATDIHMGYIIGNKRLSDLVASLNAIEADVIIFGGDLIDHNMVPVTYHNMGEILSQL
                     QAPLGVWAVTGNHEYIGNAESAVDYLSRYGIQFVRDTVVNVQGIQLVGREDAQKINFT
                     QVNRKSLETIFEQVDSKLPIIVIDHQPLEQDRVVALGADLMVSGHTHQGQLWPFKILT
                     KLIYKIEDGLVQKGNTHLYVSPGYGTWGPPVRIGNRPEIAVFRLIFD"
     gene            complement(1271..2086)
                     /locus_tag="PLJ82_07950"
     CDS             complement(1271..2086)
                     /locus_tag="PLJ82_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOR41473.1"
                     /translation="MLHPALRNYGYAIVYVLLFIVVTSAMALSLYPFYLHISLGWLLL
                     DALVFAAIYAVIGIPLWMVIQFVDFSKYTVIQQVIYMVSLCLLTLFVWLAISYVLLYL
                     LVPIDIFDVLVNAVFVRIVVGVLLYICVWQLYKSLLFGVNNEDSIIEKTSETPIDSSP
                     KPMLEKVVIKEGSTIRVISLQDIVYLQAEGDYVMIRTLDGSFIKEQTMKSFEQQLPPQ
                     QFVRVHRSYIVALAALKGVELYEKQQYLLHLKNGEKIKMSINGYKLLKQQLAL"
     gene            complement(2099..2881)
                     /locus_tag="PLJ82_07955"
     CDS             complement(2099..2881)
                     /locus_tag="PLJ82_07955"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOR41474.1"
                     /translation="MKTTEKQTNKMSSNGFGFALLLIVIGAVLLLSKLQIIPAAYNDV
                     LFKWPMLLLILGIIAFVKKHITTGLVLWIVGGFFIIPIIGAVPNSFIGAVPANFTSVY
                     WPVLLILGGVLFVFHRLFGTSCCSHKHKANFHTEFKTFSQTSSSSAGNPDYMEKNCAF
                     GSIEHIVLEPEFKGGKINNAFGETIIDLRKTTLKEGETLLDIQCMFGAVVIYVPEHWQ
                     VKIKTNAMFGAFEDKRISKGTDSASTLVILGSVMFGGAELKN"
BASE COUNT         1045 a          600 c          482 g          928 t
ORIGIN      
        1 gttataataa aaaaaattta tcaatcaaaa attaacctaa acacagcaat ttcgggtctg
       61 ttgcctatgc gtacaggagg accccaagtg ccgtatcctg gcgaaacata caaatgggta
      121 ttgccttttt gtactaaccc atcttcaatt ttataaataa gtttagtcaa gattttaaag
      181 ggccaaagtt gcccctgatg ggtatgtcct gaaaccatca aatcagcccc caaagccact
      241 actctatcct gctctaaggg ttggtggtca attactatga tgggcaactt gctatctact
      301 tgttcaaaga tggtttctag cgattttctg ttgacttgcg taaaattaat cttctgtgca
      361 tcttccctac ctactaattg aataccctgc acattgacta cagtgtcgcg tacaaactga
      421 atgccataac gagataaata atctaccgca ctctctgcat taccaatata ttcgtgattg
      481 cctgtaacag cccatacgcc caaaggagct tgtagttggc tcaatatctc tcccatattg
      541 tggtatgtaa caggcaccat attatgatca atcaaatctc ctccaaaaat aatcacatcg
      601 gcttcaattg catttagtga tgccactaaa tcactaagtc tcttgttacc gataatatat
      661 cccatatgaa tatctgtagc caaagcaaca gtaagttgtt tttttcctgc tacttgtttg
      721 ttgatacgca cctcatattc attaacggtg ggataacgag cattgatata ccctgcggtt
      781 actaaagaaa tggtgcttac ggcaactacc acaaacaacc gaaatggtgt ggctatttct
      841 aaaaacgata caggcaatag cgatgtaagc ctgtgtagca aggcaaatat atcaatcaca
      901 agtacgaaca acaaggcata aaaaaacaaa gcaagccata cagaacctat gccactaatc
      961 accgtagtga gattgaccca aggtgtacgt tctaaaaact gaccgattaa aaacgatacg
     1021 ctacacaacc aaaataacac cgtaaaagta tttctcgcca ttttattggg taaagctaca
     1081 ttagccctgt gatacacgta agcatttagt aataaataaa ttacactaat taccgatatg
     1141 ataatgacaa aatagatatt tttcatccgt aaataatttt tagtttaagg ttggatggaa
     1201 ctccatgttt gctattttca ttggtgtttg tgttttttcg caaacatgtt cgtttcataa
     1261 ttggtactta ttagagtgca agttgctgtt ttaataattt gtatccattg atactcattt
     1321 taattttctc tccatttttt agatgcagca aatattgttg tttttcatac aattcaaccc
     1381 cttttaaagc tgctaaagct actatatacg aacgatgtac acgcacaaat tgttgtgggg
     1441 gtaattgttg ttcaaaagat ttcatggttt gctctttgat aaaactccca tcaagagttc
     1501 ttatcattac ataatcgcct tcagcttgca aataaacaat atcttgaagc gaaattacac
     1561 gaattgtgct accttctttt atcacaactt tttcaagcat aggttttggg ctagagtcaa
     1621 tgggagtttc agatgttttt tcgataatcg aatcttcgtt gtttacccca aaaagtaagc
     1681 ttttatacaa ctgccataca cagatatata gtaatactcc caccacaata cgcacaaaaa
     1741 cagcgtttac caatacatca aatatatcta ttggaaccaa taaatacaaa agaacatacg
     1801 aaatagcaag ccatacaaat agggtgagca aacacaacga aaccatatag ataacttgct
     1861 gaataactgt atattttgag aaatctacaa actgaataac catccaaaga ggtatgccaa
     1921 ttacagcata gattgcagca aacacaaggg catcaagcaa caaccatcct agcgaaatat
     1981 gtaaataaaa gggatatagg gataatgcca tagcactcgt tactacaata aatagtaaca
     2041 catacactat ggcataaccg taatttctga gtgctggatg aagcatacgt ataaattatt
     2101 agtttttaag ttcagctccg ccaaacatta ccgagcctaa aatcaccaat gtacttgcac
     2161 tatctgttcc cttactgatg cgtttatctt caaaagctcc aaacattgca ttggttttaa
     2221 tttttacttg ccaatgttct ggtacataaa taactaccgc accaaacata cattgaatat
     2281 ctaataaggt ttcgccttct tttaaggtag ttttacgcaa atcaatgatc gtttcaccaa
     2341 aagcattatt aatttttcct cccttaaatt cgggttccaa cacaatgtgt tcaatactac
     2401 caaatgcaca atttttttcc atataatcag gatttcctgc cgaagaagag cttgtttgcg
     2461 aaaaggtttt aaactctgtg tgaaaattgg ctttgtgttt gtgagaacaa caactagtgc
     2521 caaacaaacg atgaaacacg aataatacac caccaagaat taagagaaca ggccaataca
     2581 ccgaggtaaa gtttgctggc acagctccta taaaactatt gggtacagct cctatgatgg
     2641 ggataataaa aaaaccaccc acaatccata gtaccaatcc agttgtaata tgttttttta
     2701 caaaagcaat aatacctaaa ataagcaata acatgggcca tttaaagagc acatcgttat
     2761 atgccgcagg aattatttgc agtttagata atagtaaaac agctcctatt acaattaata
     2821 atagagcaaa gccaaaacca ttgcttgaca tcttgtttgt ttgtttttct gttgttttca
     2881 tgttaccttg tttttaatga ataatgtgtt gtgtattttg tacaatacaa aagtagatgt
     2941 atctacaagt gttaaaaaca caaaaccgat taacagcaca aaattcccga taaaaatacc
     3001 gattttttcg acaaaggagt aaaatataaa agaaattaat gttttttatg gcgaa
//
LOCUS       DAOSRP010000150         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_29442_length_3021_cov_3.991908, whole genome shotgun sequence.
ACCESSION   DAOSRP010000150 DAOSRP010000000
VERSION     DAOSRP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29442_length_3021_cov_3.991908"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLJ82_07960"
     CDS             <1..262
                     /locus_tag="PLJ82_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41475.1"
                     /translation="IVNTTPVGTFPEVKHCPPIPYHFLTDKHLVYDLIYNPIRTLFLH
                     QAETRGATTCNGWQMFLNQAHKAYVIWEGKDLITNKKHVEKI"
     gene            354..1136
                     /locus_tag="PLJ82_07965"
                     /pseudo
     CDS             354..1136
                     /locus_tag="PLJ82_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1337..2389
                     /locus_tag="PLJ82_07970"
     CDS             1337..2389
                     /locus_tag="PLJ82_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOR41476.1"
                     /translation="MKKLHKILIMSMLLISLSNCVTKIPEPPKDIDSDFVEDHTFDTS
                     ILIVFDDNSVTATPSNLSDITFTINNGHILVDSHRKNVEYILSGTTNNGSFKLYSTSK
                     CKLTLDNVTIVSSNSPAINIQTSKSIFVHLADNSENALSDKDPYSIVELEDAKGTLFS
                     EGQLIFSGNGSLQVTSKQAHGIITDDYIHIRSGEIRVNEAVKDGISAKDHILIDGGVI
                     DLSVSDDGISCRQGYITIEDGNITVKSKDEGIVAGYDPETDLVIEGVVPDITINGGAI
                     TITTYGEKGHGIKSVGMITFNGGNTDITTYGIKSDGIDCDGGTITINGGTLSITASDD
                     CMNNEPVITAGEYTCL"
     gene            2415..2885
                     /locus_tag="PLJ82_07975"
     CDS             2415..2885
                     /locus_tag="PLJ82_07975"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41477.1"
                     /translation="MKKIISSILLAYIFVSSGFAMTHMRVEKNDASEEVIQLNTIRTI
                     RFQGENVQFNYKNGNSDLFAANTLKSLQFSSIPSTIEEKTSAELFFYPNPATDYIYLK
                     NLATEEAMISIYQIDGVIVMKGKQSLHTGIYVGNLASGLYLLEVNKQTYKFRKL"
BASE COUNT         1074 a          480 c          549 g          918 t
ORIGIN      
        1 tattgtgaac accacccccg taggaacatt ccccgaagta aaacattgtc ctcctatacc
       61 ctatcatttt cttacagata aacacttagt atatgattta atctacaacc caatacgtac
      121 gctttttcta caccaagccg aaacacgagg agcaacgacc tgtaacggat ggcaaatgtt
      181 tttaaatcaa gcacataagg cttatgtaat ttgggagggc aaagacctta ttacaaacaa
      241 aaagcatgtt gaaaaaatat gagattgata gaaaaaaata aaaaaagagg gatttagttt
      301 ggaacttatt gaacaaaagg agtaattttg tgcaatttta ataaacagaa aacatgggaa
      361 ataacttact aaaaggaaaa agaggaatta tcttcggtgc attaaacgat atgtctattg
      421 catggaaagt agctgaaaga gtagtagaag aaggtgctac cattactctt accaacacac
      481 ctttagctgt acgtatgggt acaactaacg aattaggcaa gaaattaaat gctcaaatta
      541 tcccagccga tgctacaaac gtagccgatt tggaaaatgt atttgctgaa tcaataaaaa
      601 tcttaggtgg taaaattgat tttgtcttgc attcaattgg tatgtcacca aatgttcgca
      661 aaggtcgtgc ttacgatgat ttagactatg gaatgcttag tactaccctt gatatatctg
      721 ctatttcttt tcacaaagta ttgcaagtag caaaaaagat ggatgctatc aatgaatatg
      781 gttctgtaat agctttatcg tatatggctg ctcaacgcac tgtatgtgga tacaacgaca
      841 tggctgatgc aaaagcattg ctcgaatcca ttgcacgaag ttttggttat atatatggtc
      901 gtgataaaaa agtacgtatc aatactatct ctcaatcacc aacaccaact accgctggga
      961 atggcgtaaa aggattagac accttgcttg attttgcaga aggattatct cctcttggca
     1021 acgcaacagc tgcagattgt gctgattatt gcgtagtaat gtttagcgac ttgactaaaa
     1081 aagtaactat gcagaatcta cttcacgatg gaggtttttc gagcatgagt atgagtgaaa
     1141 ccgtaattga agattacaga aaaggtttag cagccaacga ataaactgaa aagagactat
     1201 tttagataga acactatttt tgttgaaaaa cgaaaatagt gttttttttt acgttaaatt
     1261 ctattttttt tgtataaaaa tgagaatatt agctaaaaag gtgtattttt gtattttgtt
     1321 aatcttaaat aattgtatga aaaaattaca caaaatactt ataatgagta tgctacttat
     1381 ctcgcttagt aattgcgtta ccaaaattcc tgagccacca aaagacattg attctgattt
     1441 tgttgaagat catacgtttg atacttctat tttgattgta tttgatgaca attctgtgac
     1501 tgcgacgcct tcaaacctca gcgatatcac ttttactatc aataacggac acatacttgt
     1561 agattctcat cgtaaaaatg tagaatatat cctctctgga acaaccaaca atggatcatt
     1621 caagttgtat agcacatcta aatgcaaact cacccttgac aatgttacta tcgtgagttc
     1681 gaattctcct gcaataaaca ttcaaacgag taaaagtatt tttgtacatt tagccgacaa
     1741 ctcggaaaac gcattatcag ataaagaccc atattctatt gtagaactag aagatgccaa
     1801 aggtacttta tttagcgaag gtcagttaat ttttagtggc aatggaagtt tgcaagtaac
     1861 tagtaaacaa gcacatggta ttataaccga cgattatatc cacatacgaa gtggggaaat
     1921 tagggtgaac gaagctgtaa aagacggcat tagtgcaaaa gaccacatct taattgatgg
     1981 aggagttatt gatcttagcg tgtctgatga tggcatttcg tgcagacaag gctatattac
     2041 gattgaagat ggcaatataa cagttaaatc aaaggatgaa ggcattgttg cagggtatga
     2101 tcctgaaaca gacttggtaa tagaaggagt agttccagac attaccataa atgggggtgc
     2161 tattacgata acaacatatg gagaaaaagg acacggaata aagtcggtag gaatgattac
     2221 ttttaacgga ggaaatacag atataacaac ttatggaata aaatcggacg gaattgattg
     2281 tgatggtggc acaataacaa tcaatggagg aactctctct atcacagcaa gtgatgattg
     2341 catgaataat gaacccgtta taacagcagg ggaatatact tgtttataaa aattaaccac
     2401 aaaattatac atctatgaaa aaaattattt ctagtattct gcttgcatac atttttgtat
     2461 ctagtggatt tgcaatgaca catatgcgtg ttgagaaaaa tgatgcatct gaagaagtta
     2521 ttcagttaaa caccattcgc acaattcgtt ttcaaggaga aaatgttcaa tttaactata
     2581 aaaatggaaa ttctgattta tttgcagcga acacattaaa aagcttacaa ttttcttcta
     2641 ttccgtctac tatagaagaa aaaacttccg ctgaattatt tttttatccc aatccagcta
     2701 ccgactatat ttatttaaaa aacttggcaa cagaagaagc tatgatttct atctatcaaa
     2761 ttgatggagt tatcgtgatg aaagggaaac aatctcttca tacaggtatt tatgttggca
     2821 atttagcaag tggactttac cttttagaag taaataaaca aacttataaa tttagaaaat
     2881 tatgaaaaag ctacttatac tattttatac actcattttt actttaaaca tatcggctca
     2941 aatgagtatt tactttcagc aaggagaagt ggatgtaaaa gtaccatttg ttgatattga
     3001 tagcattacg ttcaatcctg t
//
LOCUS       DAOSRP010000151         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_29823_length_2991_cov_4.440054, whole genome shotgun sequence.
ACCESSION   DAOSRP010000151 DAOSRP010000000
VERSION     DAOSRP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29823_length_2991_cov_4.440054"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLJ82_07980"
     CDS             complement(<1..798)
                     /locus_tag="PLJ82_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR41478.1"
                     /translation="MKTNELADILNEFAPVSLQEKWDNAGLLIDNENSYITGVLLCLD
                     VTEKVVDEAIQKKCNFILAHHPLIFKGLTKITQHTYTERCVRKAIQHNIAIYAAHTNM
                     DVVMNGVSSMMCKQLGATDLHVLMPTENGLAKLVCYVPNTYADKIKEALFMVGVGHIG
                     NYDCCSFSSTGIGSFRANNEAHPFVGECNNLHHENECRIEMILPTRLIKKAEETLKMV
                     HPYEEPAYDFIPLVNRDYSVGLGMVATLASPMNEMDFMDMLKQKFGCK"
     gene            complement(869..2875)
                     /gene="pulA"
                     /locus_tag="PLJ82_07985"
     CDS             complement(869..2875)
                     /gene="pulA"
                     /locus_tag="PLJ82_07985"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOR41479.1"
                     /translation="MRKINLFLVSVIALLLMSCVNLPKEYASYDDYPVYEGNDLEVVY
                     SPLHTKFRLWSPAAKEAKLNLYHEGLGGEPFDVVKLKYDIDGTWVATVDGDLKGKFYT
                     FQIFQQDRWWDETPGIWAKAVGVNGNRGAIIDMSETNPSGWEDVKRPAMKNFTDAVLY
                     ELHVRDFSTSEQSGMQYKGKFLAFTEMGTVNAEGKKTGVEHLKELGITHVQLLPIYDY
                     ASVDETKLDENQYNWGYDPKNYNVPEGGYSTDPYNPTARIIELKKAIQSLKNNGIRVI
                     MDVVYNHTFWGVQSHLNLTAPGYFYRFKEDGSWSNASGCGNETASERAMVRKYIIESC
                     KYWVNEYKVDGFRFDLMGIHDIETLNLLRAELDKIDPSITIHGEGWTAEWDFPLPFEK
                     RAIKQHAKQLNNIAVFSDDIRDGIRGTWDNNKVPGFMAGVHGYEESIKFGVVGATQHN
                     QLNYDSVKYAKAPYANHPTQVINYVSCHDDLCLHDKIIYSAPEGASDKEKHRFNKLAQ
                     TIVFTAQGVPFMYAGEELKRDKQRVHNTYQSPDSINQIDWTFKTTNYDIFEYYRNLIS
                     LRKQHPAFRMPTQEMVQKHLRFINVKEPALVAYTIDDYANGDSWRRILVIYNGNRYAI
                     NMAIPVENWVVVANGEEINLNGIFKHNKQEITVPASSALIMYAD"
BASE COUNT          942 a          620 c          421 g         1008 t
ORIGIN      
        1 cttgcatcca aatttttgtt tcaacatatc cataaagtcc atttcattca ttggagatgc
       61 caaagtggct accatcccta atcctacaga atagtctcta tttactaacg gaataaaatc
      121 ataagcaggc tcttcataag gatgaaccat tttgagagtt tcttcagctt tttttatcaa
      181 tcgagtgggc aaaatcatct ctatgcgaca ctcattctcg tggtgcaagt tattacattc
      241 tcccacaaat ggatgtgctt cattatttgc tcgaaaagaa ccaattcctg tagaagaaaa
      301 actacaacaa tcataattac caatatggcc tactcctacc ataaacaaag cttcctttat
      361 tttatcagca tatgtgtttg gcacataaca gactaactta gcaagtccat tttctgttgg
      421 cattaacaca tgcaaatcag ttgcccccaa ttgcttacac atcatactac tcacgccatt
      481 catcaccaca tccatattgg tatgtgcagc ataaatagca atattatgtt ggatggcttt
      541 ccgcacacat ctctctgtat atgtatgttg cgtaatttta gttaaccctt taaaaattaa
      601 cggatgatgt gctaaaataa agttgcattt tttttgaata gcctcatcta caactttttc
      661 tgtaacatct aaacaaagta acacacctgt aatataacta ttttcgttat caatcagtaa
      721 acccgcatta tcccattttt cttgtaacga aactggagca aattcattta gtatatcagc
      781 caattcgttt gttttcatag gtaaacaaaa tataagtcta tgcgtttctt ttctaagttt
      841 aacatgctaa ttaatcaact accagtattt aatcagcata cataatcagt gctgacgagg
      901 caggtactgt aatttcttgt ttgttatgct taaaaattcc attcaagttt atttcttccc
      961 cattagctac aacaacccaa ttttctacag gaattgccat gttgatagca tatctatttc
     1021 cgttataaat aactaaaata cgcctccaag aatccccatt tgcataatca tcaattgtat
     1081 aagcaacaag agctggttct ttcacattaa taaaacgtaa atgtttttgc accatttctt
     1141 gagtgggcat tctaaaagca ggatgctgtt tgcgtaagct aattaaatta cgataatatt
     1201 caaaaatatc atagtttgtc gttttaaacg tccaatcaat ttgatttatg ctatctggcg
     1261 attgataagt gttatgaacc ctttgtttat cacgtttcaa ttcttctcca gcatacataa
     1321 aaggcacgcc ttgtgcagta aatacgatgg tttgagccaa tttattaaaa cgatgtttct
     1381 ctttgtctga tgcaccttca ggagctgaat aaataatttt gtcatgcaaa cacaaatcat
     1441 catgacaaga aacataattt attacttgag tcggatgatt agcatagggt gcttttgcat
     1501 acttaacaga atcgtaatta agctgattat gctgggtagc tcctactaca ccaaacttta
     1561 tactttcttc gtatccatgc acacctgcca taaaaccagg caccttattg ttatcccaag
     1621 ttcctcttat gccatcacga atatcatcac taaatacagc aatattattt aattgtttgg
     1681 catgttgttt aatagcacgt ttctcaaaag gtaaggggaa atcccactct gctgtccatc
     1741 cttcaccatg tatagtaata gaggggtcaa ttttatctaa ttctgcacgt agtaaattta
     1801 atgtttcaat atcatggata cccatcaaat caaatcgaaa accatctact ttgtattcat
     1861 ttacccaata tttacacgat tcaataatat atttacgaac cattgctctt tctgaggctg
     1921 tttcgttacc gcatcctgac gcattagacc aacttccatc ttctttaaaa cgatagaaat
     1981 acccaggagc agttaaattt aaatgcgatt gaactcccca aaaggtatga ttataaacaa
     2041 catccataat cacacggata ccattatttt tcaatgattg aatggctttc tttaattcaa
     2101 taattcgagc cgttggatta taagggtcgg tagaataacc tccttcagga acattataat
     2161 ttttaggatc gtacccccag ttgtactgat tttcatctaa ttttgtttca tccacagagg
     2221 cataatcata tattggtaaa agttgtacat gtgttatacc taattccttt agatgttcaa
     2281 cgcccgtttt tttaccttca gcatttacag tccccatctc ggtaaaagct aaaaacttac
     2341 ctttatattg catacccgat tgttcagatg tagaaaaatc acgcacatgc aactcgtaaa
     2401 gcacagcatc agtaaaattt ttcattgctg gtcttttcac atcttcccaa ccagagggat
     2461 tggtttccga catatcaatg atagctcccc tatttccatt tacaccaaca gcttttgccc
     2521 aaattccagg agtttcgtcc caccatctat cttgctgaaa tatttgaaaa gtataaaact
     2581 taccctttaa gtcgccatca acagtagcta cccaagtacc atctatatca tattttaatt
     2641 ttacaacatc aaaaggttct cctcccaaac cttcatgata tagatttaat ttggcttctt
     2701 ttgcagcagg tgaccatagc ctaaatttag tatgtaaagg ggagtatacg acctctaaat
     2761 cattgccttc gtatactgga tagtcatcat acgaagcgta ctccttaggc aaattaacac
     2821 acgacataag caataacgca attacggaaa ccaaaaatag atttattttt ctcatgctct
     2881 tttttcttat tctgatataa aaatattgtt cctaatacta gtagaattgc tttcactttg
     2941 ctggggtttt agatattcaa aaaatcaaac gtaattcgca aaattagttt t
//
LOCUS       DAOSRP010000152         2931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_30633_length_2931_cov_12.275730, whole genome shotgun
            sequence.
ACCESSION   DAOSRP010000152 DAOSRP010000000
VERSION     DAOSRP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30633_length_2931_cov_12.275730"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..>2931)
                     /locus_tag="PLJ82_07990"
     CDS             complement(373..>2931)
                     /locus_tag="PLJ82_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41480.1"
                     /translation="KYEYALDANGNEIMEAYYSWSSGSWVGNYKYEYAYDANGNKIME
                     AYYYWSSGKWVGNYKYTEEYNNDIKITIFYDWDAVKSQWQNDEKYEYAEDDYGNIVHD
                     VYYTWNSTTNKWEGNYKELYTYNSKGWILLEEYYYDWNDTTDTWIGYEKYVYAYNDNG
                     NYILSEYYDAWNDTTNTWIGSYKEVYDYNTKGWTILYEYYNDWNDTTNTWIGNYKEVY
                     AYDSDNNLLLEEFYNWSDSTGTWIGADKFTRTYAHGNELNFYHYEWNTVSNNWQEQEI
                     EKTIGTTHPNGGISLRMAYQWNGSTWDTVAKWETMAYDTLGNMLETEVIFFTYTSPIW
                     DELQKIIYTWDSNGKWTEIIIYNYNSGTWEENFKVEQNHDIYGNLSMEAYYYWSSGAW
                     VGNYKYEYAYDANGKQTMEAYYYWSSGKWKGNYKYERVYDVNGNQIMYASYNWNDSVW
                     VGNYKYEGAYDANGYETLDAYYTWGDSVWVGYYKYEYGYDANGVQIMKAYYDDWSNGK
                     WIGNYRNEWANDANGNLLFSQYQDGWDSTWKYGYKYVNAYDINENQILSEYSVWDTIS
                     KTWVKVNRQERAFKDTYIYFTTDFPLYNRYYEWDSTAWELQKSTEWIYNTVGDIIEVV
                     YSELFNDSLKVKTRMLTTTDTTHLRASLVFDEQWEIMFSQHLRTDYYKYDSVQTIIKH
                     DSMLYYWSSINVWSPPTYTISGTVTVDGDPLKGVQITYATSKSVTTNSSGEYTVSVDS
                     GTTVVLVPSKAGYVFTPTSITCSNVSSNLYNKDFQATDVGIADVDNTNTELVVYPNPT
                     KGIVYISSECDIKLYDIQGKKLQEIRGNQIDISSYPQGMYFLKVEGKTLKIVKE"
BASE COUNT          888 a          590 c          395 g         1058 t
ORIGIN      
        1 gacacttatc attagactaa gcgtcgctat cataaaaaat tgactgtttt tcataattgt
       61 tattatttta aatttataag caaaaataca taaatatttg atacaatata acattttttt
      121 caatttagct attaaattta tttagataaa ctaaaagtac tatacataaa atatttacat
      181 tttttagctt aaaaactttg cactgatttt acgttagcat atcacttaat ttaaacaaaa
      241 gacaagctac taatatgaca aaagcggtaa aattttctaa aaaaaccact ctttcggaag
      301 aatacaaaaa aataagaagt ctccgtgaag acttcttatt attaacaatt attgaataga
      361 attaactaaa ttttattctt tcactatctt tagtgtttta ccttccactt tcaagaaata
      421 cattccttga ggataagaag aaatatcaat ttgattacct cgaatttctt gtagcttttt
      481 accttgtata tcatatagtt tgatatcaca ctcactgcta atgtacacta ttcctttggt
      541 cggattagga taaacaacta attcggtatt cgtattgtcg acatctgcaa tacctacatc
      601 agtggcttgg aaatccttat tgtaaagatt actagataca ttcgagcaag taatagaagt
      661 aggtgtaaat acatagcctg ctttagaggg taccaaaaca accgtagttc cgctatctac
      721 agacacagta tattctccgg atgaattagt agttaccgat ttactcgttg cataagtaat
      781 ttgcacacct tttaaaggat caccgtcaac agtaactgtg cctgaaatgg tataagtagg
      841 tggcgaccat acattaatgc ttgaccaata gtaaagcata gaatcatgct taataattgt
      901 ctgaacagag tcatacttat aataatccgt ccttaagtgt tgactaaaca ttatttccca
      961 ttgttcatca aatactaaac ttgcgcgtaa atgagtggta tcggtagtgg ttagcattct
     1021 cgtttttacc tttaaagaat cattgaatag ctcactataa accacctcta taatgtctcc
     1081 tacagtatta taaatccatt ccgtagattt ttgcagttcc catgccgtac tatcccattc
     1141 gtaataccta ttgtataaag gaaaatcagt cgtaaaataa atatacgtat ctttaaaagc
     1201 tctttcctgt ctattcactt ttacccatgt tttgcttata gtatcccata cggaatattc
     1261 acttagtatt tggttttcat tgatatcata ggcatttaca tacttatacc catatttcca
     1321 agtgctatcc caaccatctt gatattgact gaatagtagg ttaccattgg catcgttagc
     1381 ccattcgttt ctataatttc ctatccactt gccattactc caatcgtcgt agtaggcttt
     1441 catgatttgg acaccattgg catcatagcc atattcgtat ttataatatc ctacccaaac
     1501 actgtctccc caggtgtagt aggcatccaa ggtttcgtag ccattggcat cgtaagcccc
     1561 ttcgtattta taatttccta cccaaacact gtcgttccag ttgtagctgg catacataat
     1621 ttggttgcca ttgacatcgt aaaccctttc gtatttataa tttcctttcc atttgccgct
     1681 gctccagtaa tagtaggctt ccatggtttg cttgccattg gcatcgtagg catattcgta
     1741 tttataattt cctacccaag caccgctact ccagtaatag taggcttcca tgcttaaatt
     1801 tccgtaaata tcatgatttt gttcaacttt aaaattctct tcccaagtac cactattgta
     1861 attataaata ataatttccg tccatttacc attgctatcc caagtataaa ttattttttg
     1921 tagttcatcc catattgggg aagtataagt aaaaaatatt acttcggttt ccaacatatt
     1981 acccaaagtg tcgtaagcca tagtctccca tttagcaact gtatcccaag tacttccatt
     2041 ccactgataa gccatacgca aactgatgcc tccattggga tgggtagtgc ctattgtttt
     2101 ttctatttct tgttcttgcc aattattact tactgtattc cattcataat gataaaagtt
     2161 taattcattt ccatgtgcat aagtacgggt aaatttgtca gctcctatcc atgtaccggt
     2221 actgtcactc cagttataga attcttctaa caataaattg ttatcgctat cgtaagcata
     2281 cacctcctta taatttccta tccaagtgtt tgtagtatcg ttccaatcat tatagtattc
     2341 atacaatata gtccaacctt tggtattata atcatatacc tctttataac ttcctatcca
     2401 agtgtttgta gtatcgttcc aagcatcata gtattcagac aatatgtaat tgccattatc
     2461 attgtaagca tatacatatt tttcatatcc tatccaagtg tccgtagtat cattccaatc
     2521 ataatagtat tcttctaata aaatccaacc tttgctatta taagtatata actctttata
     2581 attaccttcc catttattgg tggtactatt ccaagtgtaa taaacatcat gcactatatt
     2641 tccgtaatca tcttcggcat attcgtattt ttcatcgttt tgccattgtg atttaacagc
     2701 gtcccaatcg taaaaaatag ttattttaat atcattattg tactcttcgg tgtatttata
     2761 atttcccacc cacttgccgc tgctccaata atagtaggct tccatgattt tattgccatt
     2821 ggcatcgtag gcatattcgt atttataatt tcctacccaa ctgccgctgc tccagctata
     2881 gtaagcttcc ataatttcgt taccgttagc atcaagagcg tattcgtatt t
//
LOCUS       DAOSRP010000153         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_30729_length_2924_cov_5.519693, whole genome shotgun sequence.
ACCESSION   DAOSRP010000153 DAOSRP010000000
VERSION     DAOSRP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30729_length_2924_cov_5.519693"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /gene="sufD"
                     /locus_tag="PLJ82_07995"
     CDS             <1..1103
                     /gene="sufD"
                     /locus_tag="PLJ82_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOR41481.1"
                     /translation="CDVPNIQSYLCFIVNDTFYANPEPKISLPKGVIVASLNEICTTH
                     PDLVKPYLFKNMDWDNDSNQAFNATFAQDGLFIYVPKNVVVDVPIQLVNLMHGNDDLL
                     AVNHNLIIVEEGASVKLLVCDHASNEKKYLANRLTEIFVGKNASYEHYKIENTHNQTM
                     NLGTVLVHQDDDSRFLSNTLTLHNGITRNNITVHVDGQNAEAIVGGLAIGDKTQKIDN
                     YVSMNHLKPNSTSLQLFKYILDDQAKGVFTGKIIIKEDAQKIAAYQTCKTICLTNTAT
                     INTKPQLEIFADDVKCSHGATVGQLDETALFYLRSRGIPINEAKLLLMYAFVHDVLEH
                     ISVDAVKDRIRLLVEKRLRGELSKCNGCVICK"
     gene            1122..2345
                     /locus_tag="PLJ82_08000"
     CDS             1122..2345
                     /locus_tag="PLJ82_08000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOR41482.1"
                     /translation="MSINNQLIRQDFPILLDKIYNKPLVYFDNAATTQKPMCVIDRIV
                     HGYTHGNANIHRGVHFLSQKATEEHENARTTVQHFIHAARSSEIIFTRGTTESINLVA
                     FSFGETFCQPGDEIIISAIEHHANIVPWQMLKDRKGIVLKVIPMLPDGTLDMPAFSKL
                     LNNKTKLVAVTHVSNVLGTINPIQEIIQLAHNSNVPVLIDGAQAVPHIAIDVQQLDVD
                     FYVFSAHKVYGPTGIGVLYGKEEWLNKMQPYQGGGEMIAKVTFEKTTYNELPFKFEAG
                     TPDYIGSTALATALEYIQQIGLNAIATYENDLLQYATAKLLQVPGLKIYGEASHKSSV
                     ISFLVQGIHHYDMGTLLDKLGIAVRTGHHCAQPIMDYYGIEGTVRASFAFYNTKEEVD
                     KLIEGVNRVVSMFKI"
     gene            2357..2782
                     /locus_tag="PLJ82_08005"
     CDS             2357..2782
                     /locus_tag="PLJ82_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOR41483.1"
                     /translation="MTIQEKQDIIIEEFSVFDDWFDKYALIIEYANKLKPLDETKKTP
                     QNIIEGCQSRVWIDASINEKGQVVFVGDSDAIIVRGILALLLYVLSEHTPQEIIESEL
                     YFIEKIGLKEHLSPTRSNGLVAMMKQLKLYSIAFSSKKG"
BASE COUNT         1028 a          469 c          555 g          872 t
ORIGIN      
        1 cttgcgatgt tcccaatata caatcgtatt tgtgttttat tgtcaatgat actttttatg
       61 ccaatcctga accaaaaatt tccttaccta agggcgtaat tgtagctagt ttaaatgaaa
      121 tttgcaccac tcaccccgat ttagtaaaac cctatctatt taaaaatatg gattgggaca
      181 atgattcaaa ccaagctttt aacgcaactt ttgctcaaga tggattgttt atttatgtgc
      241 ctaaaaatgt ggttgttgat gtgccaattc agctagtaaa cttgatgcat ggaaatgatg
      301 atttattggc agtgaatcac aatcttatta ttgtggaaga aggtgcaagt gttaaattgt
      361 tagtatgcga tcatgcttca aatgagaaaa aataccttgc caatagatta acagagattt
      421 ttgttggcaa aaatgcttct tacgagcact ataaaattga aaacacccac aatcaaacaa
      481 tgaatcttgg taccgtgtta gtgcatcaag acgatgatag tagattttta tcaaataccc
      541 ttactttgca taacggaata acccgtaata atatcacagt acatgtagac ggacaaaatg
      601 ccgaagcaat agtaggaggg ctagccattg gagataaaac tcagaagata gataactatg
      661 tgtcgatgaa tcacttaaaa ccaaactcta ctagtttgca attgtttaag tatatactgg
      721 atgatcaggc aaaaggtgtg tttacaggca aaattattat aaaagaagat gctcaaaaaa
      781 ttgctgcata tcaaacgtgc aaaactatat gtttaacaaa tacggcaaca attaacacaa
      841 aaccacaatt ggaaattttt gctgacgatg tgaaatgtag tcatggtgca accgtaggtc
      901 agctcgatga aacagctctt ttctatctcc gttcaagagg tattcctatt aatgaggcta
      961 aactgttact tatgtacgca tttgtacacg atgtgttaga acacatttca gtcgatgcag
     1021 tcaaagaccg tattagattg ttagtagaaa aaagattacg gggagaattg tctaaatgta
     1081 atggatgtgt aatttgtaaa taaggatgat aaataagaaa aatgagcata aataatcaac
     1141 ttatccgaca ggatttccca attctgttag ataaaattta caataaacca ttagtttact
     1201 tcgataatgc tgctaccaca caaaaaccaa tgtgtgtgat tgatagaatt gtacatggat
     1261 atacgcatgg gaatgcaaat atccacagag gtgttcattt tttaagccaa aaagcaaccg
     1321 aagaacatga aaacgcacgt acaactgtgc aacattttat tcatgccgca aggtcgagtg
     1381 aaataatttt tactcgaggt actactgagt ctattaattt agtggcattt tcttttggcg
     1441 aaacgttttg ccaaccaggg gatgaaatta taatttctgc tatagaacac catgcaaata
     1501 ttgttccttg gcaaatgctt aaagatagaa aagggattgt gcttaaagtt attccaatgc
     1561 taccagatgg aactttggat atgcctgctt tttcaaaact attgaataac aaaacaaaat
     1621 tagttgcagt tactcatgtt tcaaacgtgc taggaacaat aaacccaatt caagaaatta
     1681 ttcaactcgc tcataattcc aatgttccag tgttgattga tggagcacaa gctgtaccac
     1741 atattgctat tgatgtccaa caattggatg ttgattttta cgtgttttct gcacacaagg
     1801 tgtacggtcc tactggcatt ggcgtattgt atggaaaaga ggaatggtta aataaaatgc
     1861 aaccatacca aggaggagga gaaatgattg caaaggttac cttcgagaaa actacttata
     1921 acgaacttcc gttcaagttc gaagcaggta cgcccgatta tattggttct acagcccttg
     1981 ctacagcctt agaatatatc caacaaatcg gattaaacgc tattgccaca tacgaaaacg
     2041 acttactaca atatgcaaca gcgaaactgt tgcaagtacc agggttaaaa atttatggcg
     2101 aagcatcgca taaaagcagt gtgatttctt ttttagtaca aggcattcat cattacgata
     2161 tgggaactct gctcgacaag ctcggtattg cggtccgaac aggtcatcat tgtgcacagc
     2221 caattatgga ttattatggc atagagggaa cggtaagggc ttcatttgcc ttctataata
     2281 ccaaagaaga ggtagataaa ctcatagaag gagtaaatag ggtggtatct atgtttaaaa
     2341 tttaatttgt aagggtatga caattcaaga aaaacaagat ataataatag aggaattttc
     2401 agtttttgac gattggtttg ataaatatgc actcattatt gagtatgcca ataaattgaa
     2461 acctcttgat gagacaaaaa aaacaccaca aaacatcatc gaaggatgcc aaagtagggt
     2521 atggatagat gcttctatta acgaaaaagg acaagttgtg ttcgttggag atagtgatgc
     2581 aattattgta agaggtattt tagcattact tttatacgta ttatctgaac atactccgca
     2641 agaaattatc gaatcagaat tatattttat agaaaaaatt ggactaaaag aacacctttc
     2701 gcccaccaga tccaatggtt tagtggcaat gatgaagcaa ctcaagctct acagtatagc
     2761 ttttagcagt aaaaaaggat aaaataatgt tgtattatat ccttgatttt aatgcagtta
     2821 agtaatttgg ataaaaatag attaaaaata gtttcttaat tatttggaga atcgggaata
     2881 aaggcgttat ctttgcaccc gctttgagag aaaagcggta ttaa
//
LOCUS       DAOSRP010000154         2852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_31801_length_2852_cov_5.607794, whole genome shotgun sequence.
ACCESSION   DAOSRP010000154 DAOSRP010000000
VERSION     DAOSRP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31801_length_2852_cov_5.607794"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(462..>2852)
                     /locus_tag="PLJ82_08010"
     CDS             complement(462..>2852)
                     /locus_tag="PLJ82_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41484.1"
                     /translation="QHKDVLLFSYQSDSLYMAFENSIFICTASRELLAKSLIQSDKKT
                     SIFANDKAFQTVKKISSRTIPANLFVNVKQLPFVTKKWFHPDAQRIFQFTDMYADWCG
                     FDISFMEERIVIAGFSSTSQNNKQIQLFNQQEAGVNTLVQSMPQHTYFFNHSHITQLG
                     VYKKNVQKLLSFPQQQAAKQQHDQLTTSTGENPSLFFDRHFDKEIAFGYAPYTSKFDQ
                     NAFVLIKIKDEPEAIARLQRMANESSPVATSTKNGNISIIQWQSNGFAGSVFGSQYTF
                     PEEYIAIYKGILYIAPSRNMLVYLLQQKERNRTLHRSSTYASNTQSMYQFANNTLYID
                     IPSILRRMQSVFTPEKIVWINSTRALWNNFETLILQAENKRNDLQFQQLVVNYTKKTE
                     IDILEEKQADMLVENEQPNIEDTSTIHTTQSEKTETTTPESASPTPAKKTRITALKTW
                     ELALTKPIATSPQLVINQTTGLHEIMVQDTDNFLYLITSEGKLVWRIRIAGRIISDIH
                     QVDMYRNKRLQMVFNTAEKLYIIDRSGKNVNNFPIIFATKATAGVAVFDYNNNGDYRF
                     FIPLANKRIALYKKDGSQPSDWKFKQSTELVNKPIQYFRSNGMDYLVASTNKKSYFLH
                     RRGNERLIPEKPVISSSNNNFSLIYTGGIEKFVTTDKNGNIVLINPNKKVDKVQLRTW
                     SSQHAFTTFTHQKTNYYVFVDNKRIEIYDEEFNSVMLYEDIQTKKPSIMVWENLLATY
                     NEKSKEIVLVDLIARQVVANDIKTDKNLFYIGQLKPYKKTCVVVSEKNKVIHYVLN"
BASE COUNT          777 a          482 c          561 g         1032 t
ORIGIN      
        1 gggaatagaa catataaaca ttgtagattg gttgttgaaa aaagaatgaa cacaaccccc
       61 tgttcgccgt agcgtgaagt ttgataatct gggtgttcag catagcgttt cgctatgtcg
      121 tagttatacc gattgcacaa acaatatagc ccaatttcga caaagtctca ttgccccatt
      181 tgtttgtccc gctatgcggg atgttccata cgcatttcga aatactcaat tggtattaaa
      241 aggaaagctc cagctttcca tattcgaacc aaaatcaaag ctgtgccttg cctgaacaac
      301 aaaagcatca gaaaagaaac gccatgcact tagcgtgtag tgcgaggtta agagctacgg
      361 tgaaccggtg attcataatt ggatagcgaa ggtcatattt gatccacaaa tttattccgt
      421 tatacatcga atatacaaga agaataatta aaaaagcctt gttagttcaa tacataatgt
      481 attaccttat ttttttcgct tactactaca caggtttttt tgtatggttt taattgtccg
      541 atgtagaata gatttttgtc cgtttttata tcgttcgcta ctacttgtcg agctattaaa
      601 tctactaata cgatttcttt tgatttttcg ttgtaggtag caagtaaatt ttcccacacc
      661 ataatgcttg gtttttttgt ctgtatgtct tcgtacagca ttacactgtt aaactcttcg
      721 tcgtatattt cgatgcgttt attatctaca aatacataat agtttgtttt ttggtgtgta
      781 aatgttgtaa aggcatgttg gctactccaa gtgcgtagtt gcaccttatc cactttctta
      841 tttggattaa taagtactat atttccattt ttatctgtag taacaaattt ttctattcct
      901 ccagtatata tgagtgagaa attattgttt gaggagctta ttacaggttt ctccggaatt
      961 aatcgttcat tgcctctgcg gtgtaagaaa tagctttttt tattcgtact tgccactaaa
     1021 taatccatgc cattacttct aaaatattgt ataggtttgt ttacgagttc tgtgctttgc
     1081 ttaaatttcc agtcgcttgg ttgtgagcca tcttttttgt acagggcaat tcttttgttg
     1141 gcaagtggaa taaaaaagcg ataatcgcca ttgttgttat agtcgaatac agccactcca
     1201 gcagtagctt ttgtcgcaaa aattataggg aaattgttta cattcttacc actacggtcg
     1261 atgatgtata gtttttcggc ggtattaaac accatttgca accttttgtt tctgtacata
     1321 tccacttgat ggatatcgct aataatgcgt ccagcaatgc gaatacgcca tacgagtttt
     1381 ccttcactgg taattaaata taagaagtta tcagtgtctt gcaccattat ttcgtgtagt
     1441 ccagttgttt ggtttatgac cagttgaggg ctagtagcta ttggtttagt aagagctaac
     1501 tcccatgttt tgagagctgt tatccttgtt tttttagcag gggtagggct tgccgattcg
     1561 ggggttgttg tttcagtctt ttctgattgg gtggtatgta tagtactagt gtcttctata
     1621 tttggttgct cattctctac taacatatcc gcttgttttt cttccaatat gtctatttct
     1681 gtttttttcg tgtagttgac cactagttgt tgaaattgca aatcatttcg tttattttct
     1741 gcttgcagga tgagtgtttc aaaattattc cacagtgcac gtgtactgtt tatccacaca
     1801 attttttcgg gcgtaaatac cgattgcata cgtcttagta tagaaggaat gtcgatgtaa
     1861 agggtattgt tggcaaactg gtacattgat tgtgtattgc tagcataggt agacgaacga
     1921 tgcaaggttc tgttacgttc tttttgttgt aataagtaca caagcatatt acgcgatgga
     1981 gcaatgtata aaattccttt gtaaatggcg atatattctt cgggaaaagt atactgagag
     2041 ccaaacacac ttccagcaaa tccattgctt tgccattgta taattgaaat atttccattt
     2101 tttgtagagg tggcaacggg agaactttca ttcgccattc gttgtaatcg ggcaattgct
     2161 tcgggttcat cttttatttt aatcagcaca aatgcgtttt ggtcaaattt gctcgtatag
     2221 ggagcatagc cgaaagcgat ctctttgtca aaatgacgat caaaaaacaa actcggattt
     2281 tcacccgtac ttgtggtgag ttgatcatgt tgttgttttg ctgcttgctg ttgaggaaac
     2341 gagagtaatt tttgtacgtt ttttttgtat acccctaatt gggtgatatg tgaatgatta
     2401 aaaaaatagg tgtgttgagg catgctttgt accaaggtat taactccagc ttcttgttgg
     2461 ttaaataatt gtatctgttt gttgttttgg ctagtgctcg agaaaccagc aattacgata
     2521 cgttcctcca taaaactaat atcgaatcca caccagtcag catacatatc cgtaaactga
     2581 aaaatacgtt gagcatcggg gtgaaaccat ttctttgtta cgaagggtaa ttgttttaca
     2641 ttgacaaaca aattagctgg aatggttcta ctcgaaattt tttttacagt ctgaaaagct
     2701 ttatcatttg caaaaatgga tgtttttttg tcgctttgaa tcagagattt ggcaagtaat
     2761 tcgcgtgaag ctgtacatat aaaaatgcta ttttcaaatg ccatatatag gctatctgat
     2821 tgatatgaaa agagaaggac atctttgtgt tg
//
LOCUS       DAOSRP010000155         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_32029_length_2835_cov_4.951439, whole genome shotgun sequence.
ACCESSION   DAOSRP010000155 DAOSRP010000000
VERSION     DAOSRP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32029_length_2835_cov_4.951439"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLJ82_08015"
     CDS             complement(<1..183)
                     /locus_tag="PLJ82_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41485.1"
                     /translation="MNKILHKLLYFTLLLLIFQLHTACNNKKQYDGRIEKEYTWTTLH
                     RFSVTNGYLVKAFINDT"
     gene            complement(193..423)
                     /locus_tag="PLJ82_08020"
     CDS             complement(193..423)
                     /locus_tag="PLJ82_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR41486.1"
                     /translation="MAIIVNLDVMMAKRKISLNDLSEKVGITTANLSILKTGKAKAIR
                     FQTLEAICHVLDCQPGDILEFVDDDMSTNNEK"
     gene            complement(455..1045)
                     /locus_tag="PLJ82_08025"
     CDS             complement(455..1045)
                     /locus_tag="PLJ82_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41487.1"
                     /translation="MKNKKIFLLIASISLLAGVLIYLINNTSQWQLIGSIICLTAILL
                     TDIYYFWLFFNKSNFYIPSSQDSVRWKKWTLLALYCANVLFIIAILDTIYSQSYNFDA
                     YQIVLLLLIAHVGIHTFIHNKCGIVIGKYKIYVISKGGRINSVSYDAIKACVVNTKEV
                     AILTNKNKSLYVEINSNTHADLIKEKLSTKIDIKIV"
     gene            complement(1140..1808)
                     /locus_tag="PLJ82_08030"
     CDS             complement(1140..1808)
                     /locus_tag="PLJ82_08030"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOR41488.1"
                     /translation="MIRFKILCALLFSISLYYFAIQPVITMLSVYDITELKNVIPEKP
                     RLTISQLVLAPTSIDFEGEIIGENTKEPGEIRLIKDPYGEQVIYFTPNDITFFKDNST
                     LKIINILSIVSSIILLALSVSIFVLFIKVINAFRKSEIFDRRIITKISWIGICLLSIG
                     VVLPLTSYVYYLLLKDIIAVEGYRLTTPFVEWGVILFGIAVLVINEILRKATEIKEEQ
                     DLTI"
     gene            complement(1810..2421)
                     /locus_tag="PLJ82_08035"
     CDS             complement(1810..2421)
                     /locus_tag="PLJ82_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41489.1"
                     /translation="MPTLTIKRNYDLNFSALPISINSQHYKIKNGGELMVDVPLGKCS
                     VVAKDSFGLQGNTLLEITENKPIKLYLQPRVSLLTSILLMVGMIYFYMLYIINISNTP
                     WWFILSISFPVIYQASCMLYKSNRYYSFSQANTKRVPSIIGALYLVFMLLSYYSYIFF
                     TNWLIQEHLFRAFAAFIGILGFVGLFSIGAYHLLTHKQQNKKQ"
BASE COUNT          946 a          426 c          418 g         1045 t
ORIGIN      
        1 tgtgtcgtta ataaaagctt ttaccaaata tccattagta acagaaaaac gatgtaatgt
       61 tgtccatgta tattcttttt ctattcttcc atcatattgc tttttgttgt tgcatgcagt
      121 atgtagctga aatattagca acagtaaggt aaagtagagt aatttgtgca aaatcttgtt
      181 cattattttt tattattttt cattgttggt actcatatca tcatctacaa actcgagtat
      241 atcaccaggc tggcaatcca atacatggca tatcgcttct aaggtttgaa agcgtatagc
      301 ttttgctttt cctgttttta aaatcgataa attagccgta gtaatgccca ctttttccga
      361 taggtcgttg agcgatattt ttcgctttgc catcattacg tctaaattta caattattgc
      421 catattaaaa aaaacaaatt tatgtattag ccaattatac aatttttata tcaatttttg
      481 tacttagctt ttcttttata aggtcagcat gtgtgttgct gttaatttct acatacagcg
      541 atttattttt gtttgttaaa atcgctactt cctttgtatt aaccacacac gccttaatag
      601 catcgtacga aacactatta attctacccc cttttgatat tacatatatt ttatattttc
      661 caataacgat accacattta ttatgaataa aagtatgtat tcccacatga gcgattaaaa
      721 gcaacaaaac tatttgatag gcatcaaaat tataactttg tgaatagatg gtgtccaaaa
      781 tagctataat aaacagtaca tttgcacaat ataaagccag caaagtccat tttttccatc
      841 gtacagaatc ttgagagctt ggtatataga agtttgattt gttgaaaaat aaccaaaaat
      901 aatatatatc agtcagtagt atcgctgtta agcatataat gctacctatc aattgccatt
      961 gagatgtgtt attgataagg taaattagga caccagctag taagctaata ctagctatta
     1021 atagaaatat ttttttattt ttcatattta tatatgtagc gttttattaa tattatagca
     1081 tcccaaaggt tattatttta aacatcattc ctgtgtattg atatataatg ccttgacaat
     1141 taaatggtta aatcttgttc ttcttttatt tctgttgctt tgcgtaatat ttcgttgatt
     1201 accaatacag ctattccgaa taaaataact ccccattcaa caaaaggtgt agttaatctg
     1261 tagccttcaa ctgcaataat atcttttagt aaaaggtaat atacatagct cgttaatgga
     1321 agtacgactc caatagataa taaacaaata ccaatccatg atatttttgt tataattctt
     1381 ctatcgaata tttcgctttt tctgaaagca ttaattactt tgataaacaa cacaaaaatg
     1441 gatacagaaa gtgctaataa aattatacta gacacgatgg agagaatatt tattattttt
     1501 aatgtactgt tatctttaaa gaatgtaata tcatttggcg taaaataaat gacttgctcg
     1561 ccatatgggt cttttattag tcgaatttct ccaggctctt ttgtattctc accaattatt
     1621 tcaccttcaa aatcaattga agtaggggct aatactaact ggctaatggt tagtctgggt
     1681 ttctcaggaa ttacattttt caattcagtg atatcataca cagataacat tgtaataaca
     1741 ggctgtatag caaagtaata caaactaatg ctgaaaagaa gagcacataa aattttaaac
     1801 cgtatcatat tattgttttt tattttgttg tttgtgtgtt aatagatgat aagctcctat
     1861 tgagaataat ccaacaaaac ctaggattcc tataaatgca gcaaatgctc tgaaaagatg
     1921 ctcttggata agccagttgg taaaaaatat atacgaatag taagacaaaa gcatgaatac
     1981 taaatacaag gctccaatta tactgggtac tctcttcgtg ttggcttgtg aaaatgaata
     2041 atatctgttg cttttatata acatacaact agcttgataa ataacaggaa acgaaataga
     2101 cagaataaac caccatggcg tgttagatat atttataata tagagcatat aaaaatagat
     2161 cattcccacc atcaataaga tgcttgttaa aagagacacc ctgggttgta ggtagagttt
     2221 tattggtttg ttttccgtta tttctagtag ggtgtttcct tgtaatccaa aactatcttt
     2281 ggctactaca gaacacttcc ctaacggcac atctaccatt aactcgcctc catttttaat
     2341 tttgtaatgt tggctattaa tactaattgg tagtgctgaa aagtttaagt cataattccg
     2401 cttaatagta agtgttggca tatatctttg tgattaatat agttaatatt tcggcacaaa
     2461 gatatgtata ttttattata aatcaaaata aatttatcga aaaacgataa ataattattg
     2521 atataatgaa tttcatttat tatttattgt tattgtagtt tcaatttcaa ttttcctttt
     2581 gctatgtaat tataatacga gtgccactag gtcaattctg caaagtttgc tattcaataa
     2641 tggataaatc ttttttagtg ttaagaaaag ggagtattga atattgcatt gactcaatga
     2701 taagttcaca ttgtgtaatc gtacggaatt ttagtacatt atcaaggtct tccctaattc
     2761 ttggtttaca ttgtcgctga tggaagggat attatattta tctaaattct cccaatagtt
     2821 cgttttgtaa gaatg
//
LOCUS       DAOSRP010000156         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_32123_length_2829_cov_4.571738, whole genome shotgun sequence.
ACCESSION   DAOSRP010000156 DAOSRP010000000
VERSION     DAOSRP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32123_length_2829_cov_4.571738"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLJ82_08040"
     CDS             complement(<1..797)
                     /locus_tag="PLJ82_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR41490.1"
                     /translation="MNVALFIARKLQFGNKEKKKDANPSIRIAIIGIALGLAIMLLAV
                     AIVTGFKHEIQGKVIGFGSHIQVTSLSNNTTYETPPIIFPDSLIQSIKQIDGVKHVQR
                     FATKPAIIKTDDEFQGIVIKGIDNQFNWEFFSKNLIEGETIVFNDSATSNDVIVSQDI
                     AKTLQLKVNDRFLCYFVKDGIRVRRFTVKGIYNSNFSEFDKLFILADLRHIQRLNGWE
                     ENEITGVEILVTDYRKLDDITDAVFMQTANRFEEKGNTYYTRSIKQL"
     gene            complement(826..2025)
                     /locus_tag="PLJ82_08045"
     CDS             complement(826..2025)
                     /locus_tag="PLJ82_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOR41491.1"
                     /translation="MPQTSQRGQIMPASPIRKLVPFSDKAKERGVKVYHLNIGQPDLH
                     TPEKALDAVRNIDRKILEYSPSDGLKSLRVKMLEYYQRFNIQVNLSDIIVTTGGSEAV
                     NFAFMSCLDPGDEILVPEPAYANYTAFAISAGAVVKPIVSSIEDGFALPDIARFEELI
                     TPKTKGILICNPNNPTGYLYTQEEMNQIRDLVKKHDLFLFSDEVYREFCYTGAPYISA
                     FHLEGIEENVVLFDSFSKRYSECGIRVGALVCKNKAVKESVMKFCQARLSPPLLGQIV
                     AEASIDTPKEYLEEMYNEYIERRNFLIEGLNKIPGVYSPLPMGAFYTVARLPVDDADA
                     FCSWILDEFNYKGQTVMMAPASGFYTGTTAGKNEVRLAYVLKKEELAKALVVLAKALE
                     AYPGRTI"
     gene            2422..>2829
                     /locus_tag="PLJ82_08050"
     CDS             2422..>2829
                     /locus_tag="PLJ82_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOR41492.1"
                     /translation="MKYAVDNNGYYGKFGGAYVPEILHKNVSELQERYLAIMQDSAFQ
                     KELNQLLHDYVGRPTPLYFATRLSQFVGAKVYLKREDLCHTGAHKLNNALGQILLAKR
                     MGKTRIVAETGAGQHGVATATVCALMNMECVVYM"
BASE COUNT          899 a          527 c          494 g          909 t
ORIGIN      
        1 tgcaattgtt taatgctacg agtgtagtag gtattccctt tttcctcaaa acgattcgcc
       61 gtttgcataa atacagcatc cgtaatatca tcgagctttc gataatccgt aaccaatatt
      121 tctacgccag taatctcatt ttcttcccaa ccatttaatc gctgtatatg acgtaaatca
      181 gctagtataa acagtttatc aaattcgcta aaattagaat tatatattcc ttttaccgta
      241 aaacgcctta cccgaatacc atctttcaca aaataacata ggaatcgatc atttaccttc
      301 aattgtaatg ttttggcaat atcttgcgat acaattacat catttgatgt agcactgtcg
      361 ttaaaaacaa ttgtttctcc ctcaattaag tttttagaaa aaaactccca attaaactgg
      421 ttgtcaatac cttttataac aataccttgg aattcgtcat cggttttaat aatagcaggt
      481 ttggtagcaa aacgttgcac atgttttact ccatctattt gtttaattga ttggataaga
      541 gaatcgggaa agataatcgg aggtgtttcg taggtggtgt tattgcttaa actcgttact
      601 tggatatgag aaccaaatcc aataactttg ccttgtattt cgtgtttaaa tccagtaaca
      661 atagctacag ctaagagcat gattgccaag cccaatgcaa tgccaataat ggcaatacgg
      721 atagatggat ttgcgtcttt tttcttttcc ttattgccaa attgtaattt gcgagctatg
      781 aataatgcta cattcataca ctattgtttc gtttcatata ccttgttaaa ttgttctacc
      841 tggatatgcc tctaacgctt tagccaatac cacaagagct tttgctaatt cttctttttt
      901 cagtacatat gccaaacgaa cttcattttt gccagccgtc gtgcctgtat aaaaacctga
      961 agcaggtgcc atcattacgg tttgtccttt gtagttgaac tcatctaaaa tccatgagca
     1021 aaaagcgtct gcatcatcaa ctggcaacct ggctactgta tagaaagcac ccatcggaag
     1081 cggagaatat acgcctggaa ttttatttaa tccttcaatc aagaaatttc tgcgttcgat
     1141 gtactcgtta tacatttcct ctaagtattc ttttggcgta tcaatagaag cttcggctac
     1201 tatctgtcct aataaaggtg gacttaaacg tgcttgacaa aatttcatca cactttcttt
     1261 caccgcttta tttttacaaa ctaatgcacc tacacgaata ccacattcgc tatagcgttt
     1321 tgagaaagaa tcgaataata ccacattttc ttcaatccct tctaaatgaa aagcagaaat
     1381 atagggtgct cctgtgtaac aaaactcgcg gtacacttcg tcagaaaata agaacaaatc
     1441 atgtttctta accaaatccc taatttgatt catctcctct tgcgtgtata aatagcctgt
     1501 agggttgttg ggattgcaaa ttaaaatgcc ttttgttttg ggcgtaatta gctcttcaaa
     1561 acgggcaata tcaggcaatg caaacccatc ttcaatcgaa gatacaattg gtttaaccac
     1621 agcccctgca ctaatagcaa acgcagtata gttagcgtat gcaggctctg gaactaatat
     1681 ctcatcccca gggtcaaggc aggacataaa tgcgaaattt actgcctcag agcctccagt
     1741 cgttacaata atatccgaca agtttacttg aatgttaaac cgttggtagt attccaacat
     1801 ttttacccgt aatgatttta gaccatcact ggggctatat tcaagaattt ttctatcaat
     1861 gttgcgtacc gcatctaaag ccttttcggg ggtatgcaaa tcgggctgac caatatttaa
     1921 atggtacact ttaactcctc tttcttttgc tttatcagaa aaagggacta gtttgcgaat
     1981 aggagaagca ggcattattt gcccacgttg tgacgtttga ggcatagcgt ttttatatta
     2041 tcataaagtc tgcaaagtta gtataaaatt attatttctg aaagttgtag gttttaatat
     2101 ttgtgtagaa gataaaaatt gaggttatgt cccttttata ttgaagataa aaataagcac
     2161 tattgtaaaa aaaaacatca aaaaacttgt ttatctaaaa aaaaagcaac aaatttgcat
     2221 cgtaattcaa taatgactat gaaactagta gtaaatacaa attggtggtg gcgcttacga
     2281 tacaatcatt cgtgagtggc aagcctattg tgtgtactac caacatatta ataagcatat
     2341 accgaaaggt ctgtcgaact cacgacagac cttttttgtt gataaaaatg aatgtaaact
     2401 attaaaatat ataacgaaca gatgaaatac gcagtagata acaatggata ttatggtaaa
     2461 tttggtggtg cttatgtgcc agagatatta cacaaaaatg tttctgaact tcaagagcgt
     2521 taccttgcta ttatgcaaga tagtgctttt caaaaggaac tcaaccagct gttgcatgac
     2581 tatgtaggca gacctactcc tctttatttt gctacgagat tatctcaatt tgttggggca
     2641 aaagtgtatt taaaaaggga agatttatgt catacaggtg cacataaatt aaacaatgca
     2701 cttggacaaa ttttattggc aaaacgtatg gggaaaactc gtattgtagc tgaaacaggt
     2761 gcaggacaac atggcgtagc aacagcaacg gtatgtgcgt tgatgaatat ggagtgcgta
     2821 gtgtatatg
//
LOCUS       DAOSRP010000157         2748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_33391_length_2748_cov_5.562198, whole genome shotgun sequence.
ACCESSION   DAOSRP010000157 DAOSRP010000000
VERSION     DAOSRP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33391_length_2748_cov_5.562198"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="hcp"
                     /locus_tag="PLJ82_08055"
                     /gene_synonym="priS"
     CDS             complement(<1..1126)
                     /gene="hcp"
                     /locus_tag="PLJ82_08055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOR41493.1"
                     /translation="MSMFCNQCQESAHNTGCTIKGVCGKTEDTAGIQDLVVYVCQGLA
                     YVNIEARKQGVNTLPESRKIAQSLFATITNANFDNSLLIKQIKEVIELRNSLHNKLNT
                     PLKHHTVRWNGTTDIEFVAKAALVSVQTYDADIDIRSLKQYVLYGLKGMAAYAEHAAN
                     LGQEDGDIYDFIEKALVMITKETSLDDMLSWVMKTGEYGVKVMSLLDAANTNTYGNPE
                     ITKVNIGVGKKPGILVSGHDLKDLEELLEQTKNSGIDIYTHSEMLPSHAYPMFKKYPH
                     LVGNYGNAWHKQLEEFESFNGPILFTTNCLVPPRKSTTYNNRVFTSGATGMPEWKHID
                     THLPNGQKDFTEIISLAKTCPPPTEIETGELTIGFAHNQVL"
     gene            complement(1158..2012)
                     /locus_tag="PLJ82_08060"
     CDS             complement(1158..2012)
                     /locus_tag="PLJ82_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOR41494.1"
                     /translation="MLRTIIKIDEELCNECEACVQGCHEGALQMIDGKARMVSELFCD
                     GLGACIGECPVGAITLEEREAEPYDEIATIERMIPKGENTILAHLKHLKEHGEFDYLK
                     QAVSYLRENGVKVDFAQVHNMPIHGKEESKGCPGSREVSFANTTPSFRVDTNTPATSA
                     LRQWPVQLHLLNPQAGYFKSADVVLAADCVAFSYANFHQKFLAGKTLAIACPKLDSNK
                     EIYVEKLQSMIEESLINTLTVIIMEVPCCGGLLSLASKAVSQSNRKIPMKLIVVSIKG
                     EILKEEWV"
     gene            complement(2018..2347)
                     /locus_tag="PLJ82_08065"
     CDS             complement(2018..2347)
                     /locus_tag="PLJ82_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOR41495.1"
                     /translation="MEKKEFPKAQVFTFVDSVDYAAGSIVSKILLRNEKGNLTLFAFD
                     KGEFLSEHTAPFDAIVQILDGTAEVIIDKKPHLLTTGQSIIMPANVSHALTATERFKM
                     LLIMIKG"
     gene            complement(2502..>2748)
                     /gene="asnB"
                     /locus_tag="PLJ82_08070"
     CDS             complement(2502..>2748)
                     /gene="asnB"
                     /locus_tag="PLJ82_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643963.1"
                     /note="functions in asparagine biosynthesis; converts
                     glutamine, aspartate, ATP, and water to glutamate,
                     asparagine, pyrophosphate and AMP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOR41496.1"
                     /translation="DTMMKNATHTFPINTPETKEAYLYRTLFTEHFPSRTAAETVPYE
                     RSVACSTAIALEWDAEFKKMADPSGRAVKSVHTDAYK"
BASE COUNT          739 a          520 c          503 g          986 t
ORIGIN      
        1 ccaatacctg attatgggca aagccgatgg ttaattctcc tgtttctatc tcggtaggtg
       61 gtgggcaggt ttttgctaaa gaaattattt cagtaaaatc tttttgcccg tttggtaaat
      121 gagtgtcgat atgtttccac tctggcatac ctgtagcacc agatgtaaac accctattgt
      181 tgtatgtggt agattttcgg ggaggaacta aacaattggt tgtgaatagt ataggaccat
      241 taaacgattc gaattcttct aattgcttgt gccaagcgtt tccataatta cctactaagt
      301 gaggatattt tttgaacatt ggataggcat gtgagggtaa catctcagag tgagtgtaaa
      361 tgtctattcc gctgtttttt gtttgttcca atagctcttc taaatctttt aaatcatgtc
      421 cgcttactaa gatgcctggt ttttttccta caccaatatt taccttggtt atttctggat
      481 tgccataggt gtttgtattt gctgcatcta ataatgacat tacttttaca ccatattcgc
      541 ctgttttcat tacccaagaa agcatatcgt cgaggcttgt ttcttttgta atcattacga
      601 gagctttttc aataaaatca taaatgtcgc catcctcttg tcccaaatta gcggcatgtt
      661 cggcatacgc tgccatacct tttagtccgt acaatacata ttgttttagc gaacgtatat
      721 caatatctgc atcgtaggtt tgtacactta ctagagcagc tttcgcaaca aattcaatgt
      781 cagttgtgcc attccacctt acagtatgat gtttgagagg agtgtttaat ttattatgaa
      841 gcgaatttcg aagttcaatt acttctttta tttgctttat tagtaagcta ttatcgaaat
      901 tcgcgtttgt aattgttgca aacaaacttt gtgctatttt tctactttcg ggtagggtat
      961 ttactccttg ttttcgtgcc tctatgttta cgtatgctaa accttgacat acatatacta
     1021 ctaaatcttg gataccagca gtatcctcag ttttaccaca tacacctttt atagtacagc
     1081 cagtgttgtg tgctgattct tgacattgat tacaaaacat gctcatatta ttatcgtgtt
     1141 aaatgtttat ttttgagtta tacccattct tcttttagta tttctccttt gatactcact
     1201 acaattaatt tcattggtat ttttctgttt gattgcgata ccgctttgct tgctaaagaa
     1261 agcaatccac cgcaacaagg aacttccata atgataactg taagggtatt gattaacgat
     1321 tcttctatca tcgattgtag tttttctacg tatatttcct tattgctgtc taattttgga
     1381 caggctatgg ctaaggtttt tcctgctaaa aatttttgat gaaaattggc gtaactaaat
     1441 gcaacgcaat cagcagcgag cacaacgtca gctgatttaa aatatccagc ttgtgggttt
     1501 agaaggtgca attgcacggg ccattgcctt agtgccgaag tggcaggggt gttggtgtca
     1561 acacgaaaac ttggcgttgt gttggcaaaa gatacttctc gactgcctgg acaccctttg
     1621 ctttcttctt tgccatgtat gggcatgttg tgtacttgtg caaagtctac ttttacacca
     1681 ttttcgcgta aataggaaac agcttgtttt aagtagtcaa attccccatg ctcttttaga
     1741 tgctttaagt gagctagaat agtgttttcg ccttttggaa tcatacgttc aatggttgca
     1801 atttcatcat aaggttcagc ttctctttct tctagtgtaa tggcacctac aggacattcg
     1861 ccaatacaag ctcctagccc gtcgcaaaat aattcgctta ccatacgtgc ttttccgtca
     1921 atcatttgca aggcaccttc atggcaacct tgcacacagg cttcgcattc gttacacaat
     1981 tcctcgtcaa ttttaataat tgttcttaac atagtgctta tccttttatc atgatgagta
     2041 acattttaaa acgctcggta gcagttaagg catggctcac atttgcaggc attataatac
     2101 tttgtcctgt tgttagtaaa tgtggctttt tatcaataat aacttcagct gttccatcaa
     2161 gtatttgtac aatggcgtca aatggtgcag tgtgctcact taaaaactcg cctttatcaa
     2221 aagcaaatag ggtaaggttt cctttttcgt tgcgtagaag tattttacta acaatagagc
     2281 ctgctgcata atcaacacta tctacaaatg tgaacacttg tgcttttggg aattctttct
     2341 tttccatact atcatctttt tattttgtag cacaaagata gggtgtatgg cacagataaa
     2401 gtgtaacatt tgttacactt acaaaataaa tggttgtgcc tagatttaga cacaaccatt
     2461 tagaaaataa aaactataaa aatgaaaaac taacagatac tttatttgta ggcatcagta
     2521 tgaactgatt ttacagctct tcctgatggg tctgccattt tcttaaattc agcatcccac
     2581 tccaatgcta tagcggtact acaggctaca gaccgttcgt atggaacagt ttcggctgca
     2641 gtacggcttg gaaaatgttc tgtaaatagt gtgcgatata aatatgcctc ttttgtttcg
     2701 ggtgtattaa ttggaaaagt atgagtagca tttttcatca tggtatca
//
LOCUS       DAOSRP010000158         2715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_33971_length_2715_cov_6.113158, whole genome shotgun sequence.
ACCESSION   DAOSRP010000158 DAOSRP010000000
VERSION     DAOSRP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33971_length_2715_cov_6.113158"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLJ82_08075"
     CDS             <1..901
                     /locus_tag="PLJ82_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOR41497.1"
                     /translation="KQTPAVIKGVEENYTQLNAMDEILLDGEFKLYDGVFDMGVAGVG
                     LAQTLGCGVRFVEPLQLFAPKRNTTVSLARPDNAFTSEFVFLSGIFMVQQAKYDANLL
                     FVRLPLARKLFGYDNEVTSLELRLTDGANELQVQKKIQTVLGDTFSVKNKYEQQADFF
                     RMMEVEKWITYLILSFIVLIAIFNVVGSLSMLIIEKKNDTKVLMNLGANRKLIFKIFL
                     FEGWLISILGVVVGILLGLVVCWIQIQFGILSLNGTDGAFVVEAYPVLVKWTDIVLIF
                     GTVSLLGFLAAIYPAHKLTREVK"
     gene            934..1470
                     /locus_tag="PLJ82_08080"
     CDS             934..1470
                     /locus_tag="PLJ82_08080"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOR41498.1"
                     /translation="MKKLVFIFVSALFYSVSIVAQDDSVLEYVHLFPNCVDNEESTIY
                     GGTYGEYPPEIEDAIFPGGGFVKMSQYIYTNTEMQPAYTGEINENGDSLLVTGIVRVE
                     FVVDRCGKVGRVRVVESVSELQDAEALRIVEGFPIFKPASLKDYRVKIAYIAPVYFSK
                     KVMPRKAPEIDYDSYYYW"
     gene            1473..1958
                     /locus_tag="PLJ82_08085"
     CDS             1473..1958
                     /locus_tag="PLJ82_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOR41499.1"
                     /translation="MIDIKELSLLAKKAEASSKHLFRKHKKIVEKLDDHVAVLHEKYT
                     AQYDCLACGNCCRSLGPFLTDRDIDKIAKALHLKPSAVVEKYIRIDEDGDYVFKTMPC
                     PFLMPDNYCQIYENRPKACKEYPHTDRKKFYQITNLSIKNSHVCPIVYEVLEDLKKEL
                     K"
     gene            1955..2545
                     /locus_tag="PLJ82_08090"
     CDS             1955..2545
                     /locus_tag="PLJ82_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOR41500.1"
                     /translation="MIAIGDTIVSFDVINEQFLCDIASCKGACCIEGDCGAPLTLDEV
                     GILEEILPVVWNDLSEAAKKVIDKQGVAYADIEGEMVTSIVGNKDCVFTYQEDGVCKC
                     AIEKAYNEGKTTFKKPISCHLYPIRIQKYKQFTAVNYHKWDICSAACVLGKRQKLRMY
                     QFLKEPLIRRFGSDWYNELVVVASELDKQGVKLRRK"
BASE COUNT          884 a          397 c          595 g          839 t
ORIGIN      
        1 taaacaaaca ccagctgtaa tcaaaggggt ggaagaaaat tacactcaac tcaatgctat
       61 ggatgaaatc ctgttagatg gtgagtttaa attgtatgat ggcgtatttg atatgggcgt
      121 ggcgggtgtt ggattagcac aaactttggg ttgtggagtg cgttttgtag aaccattaca
      181 gttgtttgcc cccaaacgaa atactactgt cagcttagct cgaccagata atgcgtttac
      241 gtctgaattt gtttttttat ctggtatttt tatggtgcaa caagccaaat atgatgctaa
      301 tttattgttt gtaaggttgc cattggcacg taaattattc ggttatgata atgaggttac
      361 ctctttagaa cttaggctaa ctgatggtgc aaatgagtta caagtacaaa aaaaaataca
      421 aactgtattg ggtgatactt tttcagtaaa aaataaatac gagcagcaag ctgatttttt
      481 cagaatgatg gaagtagaaa aatggattac ctatttaatt ttatctttta ttgtgctaat
      541 agctattttt aatgtagttg ggtctctctc gatgttaatt atcgaaaaga aaaatgatac
      601 caaagtatta atgaatttag gagcaaatcg aaagttgatt tttaaaatat ttttatttga
      661 aggatggctt atatctatac ttggggtagt tgtgggtatt ctactcggat tagttgtatg
      721 ttggattcag atacagtttg gcatcttatc gttgaatggt acagatggag catttgttgt
      781 agaagcatat cctgtgttgg taaagtggac agatatcgtg ttaatatttg gtacagttag
      841 ccttttaggt tttttggctg ccatttatcc tgcacataag ttgacaagag aagtaaaata
      901 aaggattaat atgtgtaaac atgagtataa aatatgaaaa agcttgtttt tatttttgtt
      961 agtgcactat tctattctgt ctcaatagta gcacaagatg attctgtgtt ggagtatgta
     1021 catttattcc ccaattgtgt agataatgaa gaatctacca tttatggtgg tacgtatggc
     1081 gagtatcctc ctgaaataga agacgctatt tttccaggag gtgggtttgt aaagatgtcg
     1141 cagtatattt atacaaacac cgaaatgcaa cctgcatata ccggcgaaat aaacgaaaat
     1201 ggagactccc tgttggttac gggtatcgtg agagtagaat ttgtagtaga taggtgtggt
     1261 aaggtagggc gtgtgcgtgt ggtagagtct gtatccgaac tgcaagatgc agaagctttg
     1321 cgaatagtcg aaggttttcc gatttttaag cctgcaagtt taaaagatta ccgagttaaa
     1381 attgcgtata tagcccctgt gtattttagc aagaaagtaa tgccacgcaa agctcctgaa
     1441 atagattatg atagttatta ttattggtaa gcatgattga tataaaagaa ttatccttat
     1501 tggcaaaaaa agccgaagct tcgtcgaaac atctttttag aaaacacaaa aagattgtag
     1561 aaaaacttga tgatcatgtg gcagttttgc acgaaaaata tactgcacag tacgattgtt
     1621 tagcttgtgg taattgctgt cgtagtttag gaccttttct tacggatagg gatattgaca
     1681 agatagccaa agccttgcac ttaaaaccat cggcagtggt agaaaagtat atccgtattg
     1741 atgaagatgg cgactatgtg tttaaaacca tgccttgtcc atttttgatg ccagataatt
     1801 attgtcagat ttacgaaaac agacccaaag cttgtaagga atatccacat actgacagaa
     1861 aaaagtttta ccaaataacc aatcttagta ttaaaaatag ccacgtttgc cccattgtgt
     1921 acgaagtgtt ggaagactta aagaaagaat taaaatgata gcgataggag ataccattgt
     1981 tagttttgat gtcataaacg aacaattttt gtgcgatata gcctcttgca aaggagcttg
     2041 ttgtatcgaa ggtgattgcg gtgcacccct tactctcgac gaagtaggta tcttagagga
     2101 aatattgcct gtggtgtgga acgatttatc agaagcagcc aaaaaagtaa ttgacaaaca
     2161 aggtgtagcg tatgctgaca tagaaggcga aatggtaaca tctattgtag gcaacaaaga
     2221 ctgcgtattt acctaccaag aagatggggt atgtaagtgt gccatcgaaa aagcttataa
     2281 cgaaggcaaa acaactttta aaaagcccat atcgtgtcat ttatatccca ttcgcataca
     2341 aaagtacaag caatttaccg ccgttaatta ccataaatgg gatatatgca gtgccgcttg
     2401 tgtattgggc aagagacaga aattgcgtat gtatcaattc ttaaaagaac cgcttatacg
     2461 ccgttttggt agcgattggt acaacgaatt ggtagtagta gcttcagaac tcgataaaca
     2521 aggggtgaaa ttacgtagaa aataagcaaa aagtggcatt ttttttgttt cgatgttgca
     2581 caaaacaaaa aatggcagta tatttgcacg ctcaattaca aaagcaacta ttggaaatgc
     2641 gattccgata aaacaaaaat gcgaaaatag ctcagttggt agagcataac cttgccaagg
     2701 ttagggtcgc gggtt
//
LOCUS       DAOSRP010000159         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_35538_length_2626_cov_5.114741, whole genome shotgun sequence.
ACCESSION   DAOSRP010000159 DAOSRP010000000
VERSION     DAOSRP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35538_length_2626_cov_5.114741"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PLJ82_08095"
     CDS             <1..1046
                     /locus_tag="PLJ82_08095"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOR41501.1"
                     /translation="MIEGEILEHTNAIEYILGILISPKYGCLKSLEEIDAVGHRVVHG
                     GERFNASVRITQEVKTQIEQCIQMAPLHNPPNLSGIEAIELLIPNVPQVAVFDTAFHQ
                     TMPKHAYIYGIPYSLYKKHGVRRYGFHGTSHRYVSKRVCEFLGVDIQSQKIITCHIGN
                     GGSITAIRNGESVDTSMGFTPLEGLLMGTRCGDLDPGVIQFLMDKEMIGTMSVSSLLN
                     KYSGVLGVSGISSDMRDLETAAISGDKRAILALKIYSYRIKKYIGAYAAALGGVDIVL
                     FTGGVGENQISMRAQICEGLEYMGLEVDLERNKVQGQETLISKDSSRVKVVVIPTDEE
                     FMIASDTEEIVSK"
     gene            complement(1082..1849)
                     /locus_tag="PLJ82_08100"
     CDS             complement(1082..1849)
                     /locus_tag="PLJ82_08100"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOR41502.1"
                     /translation="MNKKIYFVSDAHLGSLAYTNDTYREKKLVAWLDAIKHDAAAIYL
                     VGDIFDFWYEYKYVIPKGFVRLFGKIAELCDSGIPIHFFIGNHDIWTYGYLEKELGMQ
                     VHKQPLLTEFNGKRFYVAHGNGLGKNPFVVQMIEKIFHSTSVRFFYNMIHPSINMRLG
                     LAWSKKNREKKRQQEITFYGENKENLIVFAKEYVKHTPVDFFVFGHRHILLNFMIQKS
                     TQVIFLGDWIEHFSYGVFDGENFSLEIFDVEPKNPNE"
     gene            1989..>2626
                     /locus_tag="PLJ82_08105"
     CDS             1989..>2626
                     /locus_tag="PLJ82_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOR41503.1"
                     /translation="MFDILVKGFLIGILTSIPVGPIAMLCIQKTLNKGHMHGFFSGLG
                     AATSDTTYAMVALFGLSFVMPFIEQNQLLIQIIGSIVILLFGFFIFSKNPVAVLNKNI
                     ATKESYLQDYLASLLLTLSNPLIIFLFLGLFARFSFLSNTDNTVYSLSGIIAVFAGAS
                     TWWFVLTFVASIFRKKINIRGLGVINKLTGSVIIVLALGGLILSILGKSVNV"
BASE COUNT          817 a          397 c          506 g          906 t
ORIGIN      
        1 ttatgataga aggagaaatt ttggagcaca ccaatgcaat tgaatatata ttaggtattc
       61 ttataagtcc aaaatatggt tgcttaaaat ctttagaaga aattgatgct gttgggcatc
      121 gtgtagtaca tggtggcgaa agatttaatg ccagtgtacg tataacacaa gaagtaaaaa
      181 ctcaaattga gcagtgtatt cagatggctc cattgcataa tccaccaaat ttgagtggta
      241 ttgaggcaat tgaattgctc ataccgaatg ttccacaagt ggctgtcttt gatactgcat
      301 ttcaccaaac tatgcctaaa catgcgtata tttatggaat tccttattct ttatacaaaa
      361 aacacggtgt acgtcgttat ggatttcatg gaactagcca ccgctatgta tccaaaagag
      421 tatgcgaatt tttaggtgtt gatatacaat cgcaaaaaat aatcacttgt catataggta
      481 atggaggctc tattacagct ataagaaatg gcgagtcagt tgatacatcc atgggtttta
      541 ccccgttgga aggattactt atggggacgc gatgtgggga tttagatcca ggtgtgatcc
      601 agtttttgat ggataaagaa atgattggga ctatgtctgt ctcttcttta ttgaataaat
      661 atagtggtgt tttgggggtt tcaggtatct cgtctgatat gagagatctg gaaacagcag
      721 ctattagtgg agataaaagg gcaatactgg ctctcaaaat atattcgtat cgtatcaaaa
      781 agtatattgg agcatatgcc gcagccttgg ggggtgtaga tattgtatta tttactggtg
      841 gagtaggaga aaatcaaatc tcaatgcgtg ctcaaatatg tgaaggactg gagtatatgg
      901 gattggaagt tgacttagag cgaaacaagg tgcaggggca agaaactctc ataagtaagg
      961 atagtagccg tgtaaaagta gtagtaatac ctaccgatga agaatttatg atagcatcag
     1021 atacggagga aattgtatct aaataaaatg tttttattca tttgtatcat accccaacac
     1081 tttactcgtt ggggtttttg ggctctacgt cgaaaatttc taaactgaaa ttttctccat
     1141 caaatactcc atacgaaaaa tgctcaatcc aatcaccaag aaaaatgact tgagtagatt
     1201 tttgtatcat aaagtttagt aatatatgcc tatgtccgaa tacaaaaaaa tctacgggtg
     1261 tatgttttac gtattctttt gcaaatacaa tgaggttttc tttattttca ccataaaaag
     1321 tgatttcttg ttgacgtttt ttctctctat tttttttcga ccaagctaac cccaatcgca
     1381 tgttaatgga aggatgtatc atattataaa aaaaacggac agaagtagag tgaaatattt
     1441 tctcaatcat ttgcactacg aaagggtttt tccctaaccc attgccatgg gcaacgtaaa
     1501 atcgtttgcc attgaattcc gttaataatg gttgtttatg gacttgcatg cctaattctt
     1561 tttccaaata cccatatgtc cagatatcgt gattgccaat aaaaaaatga ataggaatgc
     1621 cgctatcaca caattcggca attttgccaa acaagcgaac aaatcctttc ggtatcacat
     1681 atttgtattc gtaccaaaaa tcaaaaatgt ctccaaccaa gtatatggca gcagcatcat
     1741 gtttgattgc atctaaccat gcaacaagtt ttttttcccg atacgtatca tttgtatatg
     1801 ctaatgagcc taaatgagca tctgatacaa aatatatttt tttattcatt gctaaatatg
     1861 taatgaggaa gtataattta tgtgtttctt gacaaaagta acagaatttt tcctactttt
     1921 agcctcttaa attaaaaaaa acttaatgat agtcagattt ctgttttttt ttatagatat
     1981 ttgcaggtat gtttgatatt ttagtaaaag ggtttttgat tggaatactg acctccatac
     2041 ctgtaggtcc tatagccatg ttgtgtattc aaaaaacatt gaataagggg catatgcatg
     2101 gttttttttc tgggttggga gcagctactt ctgataccac ctatgctatg gtggcattgt
     2161 tcggattatc atttgtaatg ccctttattg aacaaaatca attgttaatt caaataatag
     2221 gtagtattgt aattttatta tttggatttt ttattttttc aaaaaatccc gttgccgttc
     2281 ttaacaaaaa tattgctaca aaagaaagtt atttgcaaga ttatttggca tcattattat
     2341 taacgttaag taaccctttg attatttttt tatttttggg gttgtttgca cgttttagtt
     2401 ttttgagcaa taccgacaat acagtttatt ccctttctgg tattatagct gtttttgctg
     2461 gagccagtac ttggtggttt gtgctgacat ttgttgctag tattttccgc aaaaaaatca
     2521 acataagagg acttggggtt attaacaaac ttaccggaag tgtaattatt gtattagctt
     2581 taggggggtt gattttgtcg atattaggta aatctgttaa tgtgta
//
LOCUS       DAOSRP010000160         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_36454_length_2576_cov_5.524792, whole genome shotgun sequence.
ACCESSION   DAOSRP010000160 DAOSRP010000000
VERSION     DAOSRP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36454_length_2576_cov_5.524792"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..>2576)
                     /locus_tag="PLJ82_08110"
     CDS             complement(206..>2576)
                     /locus_tag="PLJ82_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41504.1"
                     /translation="RKEAEAKRSEMQEKMQPKYRQMHKTERNVMSTSNSSNTVRRKIT
                     DNFQQKDADKLALDSIVQSENTKETYRYDHNGNVIECIYYNWGINDWKPDYKYEYAYE
                     GDITTEIEYYWDGSDWEPDYKYEYAYDANGDLSMYAEYYWWKSDYKWVGDWKASVEYN
                     NDTIIVTMYVWDDFNLKWENDEKYEIADDVYGNTVYDVYYVWNDIGNKWEGVYKEVYA
                     YDSNDNLLLEEYYEWSNIAGTWKGVYKEICVYDSDDNILLEEYYEWMNNTWIEDEKYT
                     YTYSHGIILNFYYYVWNIVSKDWEKMGKIIGTTHPDGGISLRMAYEWNGSSWDTVFKW
                     ETIAYDTLGNMLETEVIYYEYNLVWEEQEKAIYAWDTNDKWTEMIIYYYNNGTWEANW
                     KAEQNYDIYGNLSMEAYYEWDGVTWVGDHKLEYAYDANGTQTMDAHYYWLADSWVGNY
                     KKEYVYDANDNQLLEQYQNDWDSTWKYGYMYIYAYDANGNRIMEEYSVWDSISRTWEK
                     VFKREMIFKNTYIYNADDFPLYEKYYEWDVAAWELQNSIEWIYNTIGDKIEVIYSELF
                     GDSLKVKLRIVATHDTTRLRTSLVFDEQPWHIMFSQCLRMDCYEYDSVQAIIVHDSTL
                     YYWSSGWLLPTYTISGKVTLDGDPLKGVKITYGTNQRVTTDSEGEYTVSYVDSGSTLV
                     LVPSKEGYTFTPTSITCSNVSSNLYDKDFQANGVGIADADNTNTELVVYPNPTKGVVY
                     ISSECDIKLYDIQGKKLQEIRGNQIDISSYPQGMYFLKVEGKILKIVKE"
BASE COUNT          754 a          560 c          345 g          917 t
ORIGIN      
        1 aaaatattta cattttttag cttaaaaact tttcactgat tttgcactca cacatcagta
       61 aattgagcta aacacaaact accgacaaag caaaaatggt aagaattttc taaaaaaacc
      121 actctttcga aagaatacaa aaaaataaga agcctccgta aagacttctt atgattaaca
      181 attattgaat agtattaact aaattttatt cttttactat ctttagtatt ttaccctcca
      241 ctttcaagaa atacattccc tgaggataag aagaaatatc aatttgattg cctcggattt
      301 cttgtaactt tttaccttgt atatcatata gtttgatatc acactcacta ctaatatata
      361 ctaccccttt ggtcggatta ggataaacaa ctaattcggt attcgtattg tcggcatctg
      421 caatacctac accattagct tggaaatctt tatcgtaaag attactagac acattcgagc
      481 aagtaataga agtcggtgta aatgtatagc cttctttaga gggtactaaa acaagcgtac
      541 ttccgctatc tacataagaa acagtgtatt ctccttctga atcagtagtt acccgttgat
      601 tagttccata agtgattttt acacctttta aggggtcgcc atcaagagta actttgcctg
      661 agatagtata agtaggcaac aaccatccgc ttgaccaata gtaaagtgta gaatcatgca
      721 caataattgc ctgaacagag tcatactcat aacagtccat tcttaagcat tgactaaaca
      781 ttatatgcca tggttgttca tcaaacacta aacttgtacg taaacgagtt gtatcatgag
      841 tagctactat tctcaatttt accttcaaag aatcaccgaa tagttcacta taaatcacct
      901 ctatcttatc tcctatagtg ttataaatcc attctataga attttgcaac tcccatgctg
      961 caacatccca ctcataatac ttttcatata aaggaaaatc atccgcattg taaatatagg
     1021 tatttttaaa aatcatttct cgtttgaaaa ctttttccca tgttctgctt atactgtccc
     1081 aaaccgaata ttcctccatt attcggttgc cattggcatc ataggcatat atatacatat
     1141 acccatattt ccaagtgcta tcccagtcat tttgatattg ctctaatagt tggttgtcgt
     1201 tggcatcata gacatattct tttttataat ttcccaccca agagtcagca agccagtaat
     1261 agtgggcatc catagtttgg gtgccattgg catcataggc gtattccaat ttatgatctc
     1321 ctacccaagt aacaccgtcc cattcgtagt aagcttccat gcttaagttt ccgtaaatat
     1381 cataattttg ttcagctttc caattcgctt cccaagtacc attattataa taatagataa
     1441 tcatttctgt ccatttatca ttggtatccc aagcataaat tgccttttct tgttcttccc
     1501 ataccaagtt atattcataa tatattactt cggtttccaa catatttccc aaagtgtcgt
     1561 aagctatggt ttcccacttg aaaaccgtat cccaactact tccattccac tcataagcca
     1621 tacgcaaact gatgcctcca tcaggatggg tagtacctat tattttcccc atcttttccc
     1681 aatccttact cactatattc catacatagt agtaaaagtt taatattatt ccatgagagt
     1741 aagtataggt gtatttttca tcttctatcc atgtattgtt catccattca tagtattctt
     1801 ccaataatat attgtcatcg ctatcgtaaa cacatatctc tttataaaca cctttccaag
     1861 ttcctgcgat attgctccat tcatagtatt cttctaataa cagattgtca ttgctatcgt
     1921 aagcatatac ctctttataa acaccttccc acttattacc gatgtcattc catacgtaat
     1981 aaacatcata cactgtattt ccataaacat cgtcggcaat ttcgtatttt tcgtcgtttt
     2041 cccattttaa attaaaatca tcccacacat acatagtgac tattatcgtg tcattgttgt
     2101 attctacgga ggctttccaa tctcccaccc acttgtagtc gcttttccac caataatatt
     2161 cggcatacat acttaggtcg ccattggcat cataggcgta ttcgtatttg tagtcaggct
     2221 cccaatcgct accatcccaa taatattcaa tctctgttgt aatgtctccc tcataggcgt
     2281 attcgtattt gtagtcaggt ttccaatcgt taatacccca attgtaataa atgcattcga
     2341 ttacattacc attatggtca taacgatatg tttctttagt attttccgac tgcactatgc
     2401 tgtctaatgc gagtttgtca gcatcttttt gttggaaatt gtcggttatt tttctcctta
     2461 cggtattact gctgtttgat gttgacatta cattcctttc cgttttatgc atttgccgat
     2521 atttaggctg cattttttct tgcatttcgg aacgctttgc ttcggcttct tttcgc
//
LOCUS       DAOSRP010000161         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_38114_length_2494_cov_3.708897, whole genome shotgun sequence.
ACCESSION   DAOSRP010000161 DAOSRP010000000
VERSION     DAOSRP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38114_length_2494_cov_3.708897"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..388
                     /locus_tag="PLJ82_08115"
     CDS             44..388
                     /locus_tag="PLJ82_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOR41505.1"
                     /translation="MSEIFPKQGELVGQGAPIMNIVDLQNCWITFNVREDMLSQIRIG
                     TELTVEIPALNKKQVKVKVNYIKALGSYATWRATKTSGEFDVKTFEVRAKPMDVIEDL
                     RPGMSVLLLDEL"
     gene            415..1596
                     /locus_tag="PLJ82_08120"
     CDS             415..1596
                     /locus_tag="PLJ82_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR41506.1"
                     /translation="MRLKQKNKGFKAVFYREWLRMATHPIYLIILVAIPLGSCLFFVT
                     LMPEGLPEKLPIGIIDHDNSMISRNTIRKIDATAQNRIVGYYLNFSEARSDMQQGKIY
                     GFIEIPVDFEKDIILGNQPKVWFYYNQSFFIAGSLTLKNLSRILATISGGVNLQSRLA
                     HGQTEKSSIAQIQAIVPDIHPIGNPHINYSVYLTDIILPGILELIVLLTSVYIVGIEL
                     KQKTSRKWLAIADNSLFKALLGKFLPYTISFVIIGLFYNVVLFKFLQYPLNTSFLWML
                     LNTGLMIVASQCVGILMIGIFPVLRDGLSFASLYGVLAFSFSGFSFPIEGMHPLLQGL
                     SVLFPLRYHFKIYQTLALNGFGWQYVAFDFVSLVLFLSLPFLICIRLKKAMIFQCYPK
                     K"
     gene            1617..>2494
                     /locus_tag="PLJ82_08125"
     CDS             1617..>2494
                     /locus_tag="PLJ82_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR41507.1"
                     /translation="MVDKLNYKSIKKAIQFTWQLTCQELKNVFTDSGVIIIFFAAGLL
                     YPLLYGFVYYNENLTNVPVAVVDNSQSAMSRELIRKIDATPDLEVSFHSPSLEEAKLL
                     FAKNKVKGIIFIPSSFSKDIQTGIQTNISAYASMASMLYYRAMYAAVNYVCLDMGQHI
                     QMNNLASTGLTQRQTQVYATPVLSEGKSLYNPKGGYASFLVPTILILVIQQTLVLGIG
                     ILAGTAREENSFHQLIPIKKKYHGTLRIVLGKSFAYLILYVFISCYNLIVIPRIFNLP
                     QLDDILTLVIFMFPLL"
BASE COUNT          798 a          358 c          448 g          890 t
ORIGIN      
        1 gcgatttgct aaaaattaat caattatttc ctaatctggt gaaatttctg aaatatttcc
       61 aaaacaagga gaattggtag ggcagggtgc cccgattatg aatatagtgg atttacagaa
      121 ttgttggata acatttaatg taagggagga tatgctctca caaatacgta ttggtacaga
      181 gctaacggta gagattccag ctttaaataa aaaacaagta aaagttaaag tgaattatat
      241 taaagcattg ggcagttatg ctacttggcg agcaactaaa acttctggag aatttgatgt
      301 gaaaacattt gaggttaggg ctaaaccaat ggacgtaatt gaagatttac gaccaggaat
      361 gagtgttctt ttgctagatg aactataatt ttaggtattc atatacatta tattatgcgg
      421 ttaaaacaaa agaataaggg attcaaggcg gtattttatc gtgaatggtt acgaatggca
      481 acacatccga tatatcttat tatccttgtt gccatacctt tgggtagctg tctatttttt
      541 gttaccttaa tgcccgaagg attgccagaa aaactaccaa ttgggattat cgatcatgat
      601 aattccatga tatctcgtaa tacaattaga aaaattgatg caactgcaca aaatcggatt
      661 gttggttact atctcaattt ttcagaagca cgatcagata tgcaacaagg taagatatat
      721 ggttttattg aaatacctgt tgattttgaa aaggatatta ttcttggcaa tcaacctaaa
      781 gtatggtttt attataacca atcatttttt attgcagggt cattaacatt aaaaaatctt
      841 agtagaatat tggcaactat ttcgggaggt gttaatttac aatcaagatt ggctcacgga
      901 caaacagaaa aatcaagtat agcccaaata caagcaattg taccagatat acaccctatt
      961 ggaaatccac acattaatta ttcggtatat ttaacagata tcattttgcc tggaattttg
     1021 gagttgatag ttttgttaac ctcggtgtat atcgtgggga tagaattgaa acaaaaaaca
     1081 agccgaaaat ggcttgccat agctgataat tctctattta aagcattatt aggtaaattt
     1141 cttccatata caatttcttt tgttattatt ggattatttt ataatgtggt tttatttaaa
     1201 tttcttcaat atccattgaa tactagtttt ctatggatgt tgctgaacac tggtttgatg
     1261 attgtggctt ctcaatgtgt gggaatttta atgattggaa tatttccagt attacgagat
     1321 ggtttaagtt ttgcgtcttt gtatggagtt ctagcttttt cattttctgg attttctttt
     1381 cctatagagg gtatgcatcc gcttttacaa gggttaagtg ttcttttccc attacgatat
     1441 cattttaaga tatatcaaac actggcttta aatggatttg gatggcagta tgtagcattt
     1501 gattttgttt ctttagtact gtttttatcg ttgccttttc ttatttgtat aagactaaaa
     1561 aaagcaatga tttttcaatg ttatcctaaa aaataaataa tagaagaatt agcacaatgg
     1621 ttgacaaact taattataaa tcaataaaaa aagcaattca attcacctgg caacttacct
     1681 gtcaggagtt gaaaaatgtt ttcacagatt cgggtgttat tattatattt tttgctgctg
     1741 gattattata tcctttgcta tatggattcg tttattataa tgagaattta acgaatgttc
     1801 ctgtagctgt agtcgataac tctcaatcag ctatgagtcg tgagttaata cgcaagatag
     1861 atgcaacacc tgatttggaa gtaagttttc attctccctc gttggaagag gcgaaattac
     1921 tttttgcaaa aaacaaagta aaaggaatta tttttattcc ttcttcattt tctaaagata
     1981 ttcagacagg aatacaaaca aatatatcag cttatgccag tatggctagt atgctgtatt
     2041 atagggcaat gtatgctgct gtaaattatg tttgcttgga catgggacaa catattcaga
     2101 tgaataatct tgcatcaaca ggattaactc agaggcaaac tcaggtatat gctactcctg
     2161 tgctgagtga gggaaagtcg ctttataatc caaaaggagg atatgcttct tttctcgttc
     2221 ctactatttt aattttagta attcagcaaa cattagtact cggtattgga atacttgctg
     2281 ggacagctcg agaagaaaat tcctttcatc aattaatccc aataaaaaag aaataccatg
     2341 gtactttgcg tattgtgcta gggaaatcat ttgcctattt gatattgtat gtatttattt
     2401 cttgttacaa tttaattgta attccccgaa tttttaattt gcctcaactt gatgatatcc
     2461 taacattggt aatttttatg tttccattat tatt
//
LOCUS       DAOSRP010000162         2471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_38629_length_2471_cov_3.630381, whole genome shotgun sequence.
ACCESSION   DAOSRP010000162 DAOSRP010000000
VERSION     DAOSRP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38629_length_2471_cov_3.630381"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..668
                     /locus_tag="PLJ82_08130"
     CDS             21..668
                     /locus_tag="PLJ82_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR41508.1"
                     /translation="MPHIIIADNQAISRFGIYQLLSKQAIQYQTTECNTKDQLVLMLR
                     QHTNAIIMLDYTLFDFLGIDDLLVLAERYPLAHWLLLSDELSPDFLQKALLSANNISV
                     VMKDATQEELLFAMQHVVRKERFVCNYVSNLLLSAKTIDANYAIQLTDTEKIILKEIA
                     LGYTTKEIAAKKFISFHTVNTHRKNIFRKLGINNLHEATKYAMRAGIIDMAEYCI"
     gene            867..2051
                     /locus_tag="PLJ82_08135"
     CDS             867..2051
                     /locus_tag="PLJ82_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41509.1"
                     /translation="MMHSKTYKWGLFIFLMLVLGIGIFVWFNTKHFYTNFEIKDELGG
                     NIFPSSILSVATTDTQVIMPSDSVYVGNPKSGIPISIRSGKAFSRVRIEIAETRFFAR
                     SVSEFVLEKENTEYLIYPDVIWNYDALRNNSQAEPISVVITAELNGKPLGQRVRTYSV
                     RSINECLLGYVSNGTKYHDTGIFFAAYVNEDHPMIDQILREALDTRIVTRFLGYQART
                     PEDVDKQVYALWNVLQRRNFQYSSVANTSLSSNVVFSQRIRTFDDALNSSQINCVDGS
                     VLFASLLRAINMEPILVRMPGHMFVGYYTNSKHSNINFLETTMIGDVNLDDFFPDEQL
                     DSTMVGKSKNQMSRITFEKSKEYANKKYEEVKENVKAEKPNYMFLVISKDVRRKIQPI
                     GK"
BASE COUNT          826 a          483 c          441 g          721 t
ORIGIN      
        1 tataagaaaa catagcaact atgccacaca tcatcatagc cgataaccaa gccataagcc
       61 gttttggaat ataccaattg ctaagcaagc aagctataca gtaccaaaca accgaatgca
      121 ataccaaaga ccaattggta ttgatgttac gccaacatac caacgcaata atcatgttgg
      181 attatacctt gtttgacttt ttaggtattg acgatttatt ggtgttagca gaacgatatc
      241 cgctagcaca ttggttgcta ttatcagacg aattaagtcc tgatttttta caaaaagcct
      301 tgttaagtgc taacaatata agcgttgtga tgaaagacgc tacgcaagaa gaactactat
      361 ttgctatgca acatgtagtg cgaaaagagc gttttgtatg caattatgta agtaatttat
      421 tgctttctgc caaaacgata gatgctaatt acgccataca gttaacagat accgaaaaaa
      481 taatactgaa agaaatagct ttgggttaca ccaccaaaga aatagctgca aaaaagttta
      541 tcagttttca taccgtaaat acacaccgaa aaaatatttt ccgcaaacta ggaattaaca
      601 acctacacga agccaccaaa tatgctatgc gagcaggcat tattgatatg gcggaatatt
      661 gtatctaaaa aggcataaca aacacaatct acatcctgta tcactgctga taaaacaatg
      721 aaaattgtac acttacaaaa aaaacttatt tttagaatct gaattttaaa cattttaaaa
      781 gattaattac taaaaattaa tccatctatt tgtgttaaaa tagactatct ttgtacattc
      841 gtatatatcg tttgaacatt ctatctatga tgcatagtaa aacttacaag tggggtctat
      901 ttatttttct gatgcttgtg ttaggaattg gaatttttgt ttggttcaac acaaaacact
      961 tttacacaaa ttttgaaata aaagatgaac taggcggaaa tatctttcct tctagcattt
     1021 tatctgtggc aactaccgat acacaagtaa ttatgcctag cgactctgtc tatgtaggga
     1081 atcctaaatc aggcattccc atttctatcc gttctggcaa ggctttcagt agagtacgta
     1141 ttgaaattgc cgaaacacgt ttctttgctc gttcggtatc tgagtttgta ctggaaaaag
     1201 aaaacactga atacctcatt tacccagatg taatttggaa ttatgatgcc ctacgcaaca
     1261 acagccaagc agaacccatc agcgtggtaa ttaccgctga actcaacgga aaaccacttg
     1321 ggcaacgcgt acgcacgtac tctgtgcgaa gcatcaacga atgcttgctt ggatatgtaa
     1381 gcaatggaac aaaataccac gatacaggaa tcttttttgc tgcttacgta aacgaagatc
     1441 atcccatgat agaccaaata ctgcgtgaag ctcttgacac acgaatcgtt actcgctttt
     1501 tgggttatca agcacggact cccgaagatg ttgacaaaca ggtatatgcc ctgtggaatg
     1561 tattacaaag gagaaatttc caatacagtt ctgtagccaa tacaagccta tcaagcaatg
     1621 tggtgttttc gcaacgaata cgtacgtttg atgatgccct aaattcatca caaattaatt
     1681 gtgtagatgg tagcgtactt tttgcctcct tactgcgagc tattaatatg gaacccattt
     1741 tggtacgcat gcctgggcac atgtttgttg ggtactatac caactccaaa cactccaaca
     1801 taaatttttt ggaaacaacc atgattggcg atgtaaacct cgacgatttt ttccccgacg
     1861 aacaactaga ttctaccatg gtaggaaaat cgaaaaacca aatgtcgcgt atcacatttg
     1921 aaaaatcaaa agaatacgcc aacaaaaaat acgaagaggt aaaagaaaac gtaaaagcag
     1981 agaaacccaa ctacatgttt ttggttatct ccaaagatgt gcgtagaaaa atacaaccta
     2041 taggaaagta aatttcttgc tccgtaaccc aataatgcct tgttgttttg tttacatgtt
     2101 acttcacaga gttacacaga gtatttaata ccccgtaggg gctacatcat aatagaaaat
     2161 acataccgat ttgtttgtta gctccagcgg agcggaatag atatctttac gtcttcttcg
     2221 tgtaagaaat agtacacaga gagacacgga ggtttcacag agtggcacgg aggtttatgt
     2281 tgctttggtt ttgggatagc aaaaatttca ctctacattg taaccctatc cgcatgttat
     2341 tatgtcgctc ctacggagct tacattacac aattcgtact tatgctatta ctatttcgct
     2401 cctacggagc ttgggtttta ccaattttta ggggaaaatg ctatttcgct tgccccatag
     2461 gggctacatc a
//
LOCUS       DAOSRP010000163         2421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_39706_length_2421_cov_4.437870, whole genome shotgun sequence.
ACCESSION   DAOSRP010000163 DAOSRP010000000
VERSION     DAOSRP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39706_length_2421_cov_4.437870"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PLJ82_08140"
     CDS             complement(<1..1279)
                     /locus_tag="PLJ82_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41510.1"
                     /translation="MEETAKNFSKQEWFKMNGGVTLNSMYMQGNVPPSSTLSYLLAGN
                     VHFQLFNTIQVPLSISLTNTGANVAYPTLPNRFSLHPSYKWATLHLGDITLPFSPYTL
                     SDHQFTGVGLELSPDKWKIAAMYGQLQRKLEYDTANVLVPAAYQRMGVGTQVRYQHEI
                     FTIGSSIFLAKDNETSLLNQPDSVGIFPQQNLASQVDISANVAPNIRLMGEYAFSLLD
                     RDMRIPSNGSRVYYQSYNMGIAYTFLKNELQLKYERIDPQYSTLGTYYSNSDYENITV
                     NVTTQLLKDKVQLATNVGVQNDDLDGTKGSRTSRFVGAVNVAYTPSQRVSINAGYSNF
                     QSHKNVKSQFNYINQYDIVQNLDTLDFVQLSQNANFMCSYVLQQTDSQNQNVQFTTNW
                     QQAKDTYIGISNPSNFMSMLNSYLMHTIQFNKTN"
     gene            complement(1511..>2421)
                     /locus_tag="PLJ82_08145"
     CDS             complement(1511..>2421)
                     /locus_tag="PLJ82_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41511.1"
                     /translation="KKGNEKVSFPVMVQPVDSFPPAMPVNLKAKIDTMGIVTLTWSPN
                     TEADLLGYRVFRSFTENGELFSLNDVAWKSTTFMDTVDVKNLNRAVYYSIAALDYRYN
                     QSEKTPILKLVKPNLIPPSSPVFNAYKATPSGIELTWVNSSSEDVESLLLYKTSMLTQ
                     KPELVGEFLPTVDTYIDKQTEFDIVYRYELVAKNSYGVRSKENPSIDVRSLTQQKTSI
                     QVSSEIQTQALLIKWDVKAKEPIRSVSIYRQSGTEPLTLWKELQPWQLSCVDKDVRIG
                     TAYGYMVKCVLVSGQTLFSTVHQVNF"
BASE COUNT          727 a          471 c          416 g          807 t
ORIGIN      
        1 agttggtttt gttaaattgt atcgtatgca tcaggtaaga attgagcatc gacataaaat
       61 ttgatggatt gcttatgcca atatacgtat cttttgcttg ctgccaattg gtagtaaatt
      121 gcacattttg gttctgactg tcagtttgtt gcaacacata cgaacacata aaattggcat
      181 tttgagacaa ctgtacaaaa tctaaggtat ctaagttttg gacaatatca tattggttga
      241 tataattaaa ttgagatttg acatttttat gcgattgaaa atttgaatac ccagcattta
      301 ttgatacacg ttggctcgga gtatacgcta cattcaccgc acctacaaat cgagaagttc
      361 tacttccttt tgtgccatct aaatcgtcgt tttgcacacc aacatttgtg gctaattgta
      421 ctttatcttt taacaactga gtagtaacat tcaccgttat attttcgtaa tcgctattgc
      481 tataatacgt gcccaaggta ctgtattgag gatcaatacg ttcatattta agttgcagct
      541 catttttcaa gaatgtatag gctataccca tattataaga ttgatagtaa accctgctcc
      601 catttgaagg aatgcgcata tctctatcga gtaaactaaa cgcatactca cccatcaatc
      661 gtatattggg agctacattg gcagagatat ctacttgtga agccaaattt tgttgtggaa
      721 aaatacctac gctatctggt tgattgagca aagaggtttc attgtctttg gctaaaaaaa
      781 tactcgaacc aatcgtaaat atttcgtgtt gataacgtac ctgtgtgcca acccccattc
      841 gctgataagc tgctggtact agtacattgg ctgtatcgta ctctaactta cgctgtaact
      901 gaccatacat tgcagcaatt ttccatttat caggactcaa ctccaagcct acccctgtaa
      961 attgatgatc ggataaggta taaggagaaa acggcaatgt aatatcgcca agatgcaagg
     1021 tagcccattt atacgatggg tgcaacgaaa aacgattggg taaggttgga tacgcgacat
     1081 tagctcctgt atttgttaat gatatactta acggaacttg aatggtatta aataattgaa
     1141 aatgaacatt gccagccaat aggtaactta gcgtactact agggggtaca ttcccctgca
     1201 tatacatact attgagggtt acgcctccgt tcattttaaa ccactcttgt ttggaaaaat
     1261 ttttggctgt ttcctccacc cgataggttt gacaatgcaa cgctataacc acgaaagaat
     1321 acacaattgt tagggccaat ttttgggctt cttgttttat gctcgttata atcacctttt
     1381 ttgcttgctt ttttatatgt attttatata tatttttacc aagatatcag ggctacgccc
     1441 ctaatttatg cttcaacact aaacattcaa cactaaacac taatcattaa acattttctc
     1501 cttttttact ttaaaaattc acttgatgaa ccgtactgaa caatgtctga cctgaaacca
     1561 atacgcattt taccatatac ccataagctg taccgattct aacatctttg tccacacacg
     1621 atagttgcca aggttgtagc tctttccata acgttaatgg ttctgttcct gattgtctat
     1681 aaatagatac tgaccgaatg ggttcttttg cctttacatc ccatttaatt agtaaggctt
     1741 gcgtttgtat ttcgctcgaa acttgaatgg atgttttttg ttgtgttagc gaacgtacat
     1801 caatgcttgg attttccttc gacctaactc catacgaatt cttagcaacc aactcatacc
     1861 gatatactat atcaaattcc gtttgtttat cgatatatgt atcaacagtc ggcaaaaatt
     1921 ctcctaccaa ttcaggcttt tgagttaaca tcgaagtttt atataacagc aaagactcca
     1981 catcttcact tgagctattt acccatgtca attcaatacc cgatggggta gctttatatg
     2041 cattaaatac aggtgaagat ggtggtataa ggttgggttt tactagcttt agtatgggag
     2101 ttttttcgga ctgattatac cgataatcaa gggctgcaat agaataataa acagctctat
     2161 ttaaattttt cacatcaaca gtatccataa atgtagtgct tttccaagcc acatcattca
     2221 aactaaataa ttcaccattt tcagtaaagg aacgaaacac acgatatccc aataaatcgg
     2281 cttcggtatt tggagaccac gtaagcgtaa cgattcccat agtatcaatt ttcgctttta
     2341 ggttaactgg catagctgga ggaaaagaat ctactggctg aaccataaca gggaaagata
     2401 ctttttcatt cccttttttg t
//
LOCUS       DAOSRP010000164         2387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_40516_length_2387_cov_4.584477, whole genome shotgun sequence.
ACCESSION   DAOSRP010000164 DAOSRP010000000
VERSION     DAOSRP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40516_length_2387_cov_4.584477"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="PLJ82_08150"
     CDS             complement(<1..1568)
                     /locus_tag="PLJ82_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOR41512.1"
                     /translation="MKKSSFLLFFLLFSLCVFGFKSTEMRLLMQSAPVFSFFSDGSYV
                     YVSADGKSIVRKDAKTDVETLVFSIEGRSQLSSIGGFILSPDQATMLVYEKTERSSSV
                     EPLNYFLVDRQRNKLEPLSEYGKQQIPLYSPNGKMIAFVRNGDVYIKRLEFGTELRVT
                     ENGLINTLSNGLPDKLYQQGFGINSSIVWSPDSKMIAFVQYDETDVPLFTHTETTTTY
                     PQLFQQRYSKPGFPITKARLFVYTIQFRKHLEMKLPDEGDNYITAVAWSSNPELMAIN
                     VLNREQNERKTIFYNPLSGVARMANQENASPAFSPLAARFLQFLPDNSFTLLSEKTGY
                     TQLYHYANNGILIRQLTNGKMAITDVYGYDTIGKQFFYQSFTKGLLNKGIFAVNLQGK
                     IRTLFDEEGVHTAYFSPQFSHFVHQYSNVNTPPYYTLYDVKMKKLGRVFDNAAMADKM
                     MKRVFPTKELLPMGWMVKPSDFDERKVYPVVVVLQETLNQWDISYAQDIANQGYIVCS
                     FSPTMYRYAGNEQE"
     gene            complement(1596..>2387)
                     /locus_tag="PLJ82_08155"
     CDS             complement(1596..>2387)
                     /locus_tag="PLJ82_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR41513.1"
                     /translation="RRGEGVGYYGATTSLAMAIGPMVSLHLYELYGNYDALFLLSFAV
                     GMVGLAIMLFIKFRCVEHIETQKEPISLDRFYLLKGTPIAFSLLTVAFSYGLIVTYVA
                     VYGITEVGLKMGASLFFVLFAMGIMFSRFSVGKLLDKGYFSGIILVGLVLAISGLVLL
                     VVIKNVYGFYISAIMLGVAYGILGPAFQLLFINLAQHNQRGTANSSYFIMWDLGIGLG
                     VLFGGNVASMASYGDSYLMAAVVLLVGLVWFRWYALSYYNQHKLR"
BASE COUNT          837 a          488 c          350 g          712 t
ORIGIN      
        1 tgttcctgct cattaccagc atatctatac atggtaggac taaaagaaca gactatatat
       61 ccctgatttg caatgtcttg tgcatacgaa atatcccact gattaagggt ttcttgtagt
      121 acaacaacaa ctggatagac ctttcgttca tcaaaatcag aaggttttac catccacccc
      181 attggcaata gttctttagt aggaaacaca cgtttcatca tcttgtctgc catcgctgca
      241 ttatcaaata cacgacctag ttttttcatt ttaacatcat acaatgtata gtatggagga
      301 gtattcacat tactatattg atgtacaaaa tgtgaaaact gtggtgaaaa ataagcggta
      361 tgtacgcctt cttcatcaaa caaggttcgt attttccctt gtaaattcac agcaaatatt
      421 cctttgttta acaatccttt agtgaatgat tgataaaaaa attgtttccc tattgtgtca
      481 tatccataca catcagtaat agccatctta ccatttgtaa gttgacgtat aagtatacca
      541 ttgtttgcat aatggtaaag ctgtgtatat cctgtttttt cacttaataa agtaaaagaa
      601 ttatcgggta aaaactgtaa aaaccgtgct gccaaaggag aaaaagctgg agaggcgttt
      661 tcttggtttg ccattctagc tacacctgac aatggattat aaaaaattgt ttttcgttca
      721 ttctgctctc tatttaaaac gttaattgcc attaattctg gattagacga ccatgctact
      781 gctgtaatat aattatctcc ttcatcaggt aatttcatct caagatgctt acgaaattgt
      841 atggtataaa caaataatct ggcttttgtt attgggaaac ctggttttga atacctttgc
      901 tgaaataact gaggatacgt agtggtagtt tccgtatgag tgaacaaagg cacatccgtc
      961 tcatcgtatt gaacaaaggc tatcatctta ctatcaggag accatacaat ggagctgttt
     1021 atcccaaaac cttgttggta taacttgtct ggcagaccat ttgataaggt attaatcaat
     1081 ccattttctg taacacgcaa ttcggttcca aattccaaac gtttgatata cacatctcca
     1141 ttacgtacaa aagcaatcat ttttccatta ggcgaataca acggtatttg ttgttttcca
     1201 tattcagaaa gaggttcaag cttattgcgt tgcctatcta ctaaaaaata attcaatggt
     1261 tcaacagaag agctacgttc tgttttttca tacaccaaca tcgttgcttg atctggacta
     1321 agaataaatc cgccaataga tgataattga gaacgtcctt ctattgaaaa cacaagcgtt
     1381 tcaacatccg ttttagcatc cttacgtaca atacttttgc catctgcaga cacatacaca
     1441 taactaccat ccgagaaaaa agaaaagaca ggtgccgatt gcatcaataa tcgcatttcg
     1501 gtacttttaa aaccaaaaac acaaagtgaa aacaaaagaa aaaacaataa aaaactagat
     1561 tttttcatac tatatctcca tttaactata taacattaac gcaatttgtg ttggttataa
     1621 tatgacaatg cataccacct aaaccatact aatccaacaa gtaaaacaac agctgccatt
     1681 aaataactat ctccataact tgccatacta gctacatttc ctccaaataa tacacccaat
     1741 ccaataccta aatcccacat aataaaataa gacgaattgg cagtacctcg ttgattgtgc
     1801 tgtgccaaat taataaaaag caactgaaac gcaggtccta ggatgccgta tgctactcct
     1861 agcataattg ctgaaatata aaacccatac acatttttta ttactaccaa taaaacaagt
     1921 ccacttattg ctaaaacaag acctactaaa ataataccag aaaaatagcc tttgtcaagc
     1981 aatttaccca ccgaaaaacg tgaaaacata atacccatag caaagagaac aaagaataaa
     2041 cttgccccca ttttcagacc cacctcagta ataccatata cagctacgta tgttacaatt
     2101 aatccatacg aaaaagctac cgttagtaat gagaaagcga taggagtacc ttttaataag
     2161 taaaagcgat cgagcgaaat tggttctttt tgagtttcta tgtgttctac acagcgaaat
     2221 ttaataaaaa gcataatagc taaccctacc attccaacag caaatgaaag caaaaataag
     2281 gcatcataat tgccatataa ttcatataaa tgcaaagaga ccatgggtcc aatagccatt
     2341 gccaaactcg ttgtagctcc ataataaccc acaccttcac cacgcct
//
LOCUS       DAOSRP010000165         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_41231_length_2357_cov_5.080799, whole genome shotgun sequence.
ACCESSION   DAOSRP010000165 DAOSRP010000000
VERSION     DAOSRP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41231_length_2357_cov_5.080799"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLJ82_08160"
     CDS             <1..450
                     /locus_tag="PLJ82_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOR41514.1"
                     /translation="QPQIFGWLDLLDMNVWVILILMLTVAGFSMISGLLILILERTNM
                     IGILKALGGKNWFIRKIFLYQAFFLVGKGMLWGNILGISLALLQQQFNIITLDPLTYY
                     VNTVPINLNLWYIVFVNALCLFFSLLVLLAPSHMIAKIEPSKAIRFE"
     gene            complement(463..843)
                     /locus_tag="PLJ82_08165"
     CDS             complement(463..843)
                     /locus_tag="PLJ82_08165"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOR41515.1"
                     /translation="MAQSYLGVWEQINNDGQKGALVRIEKSSRANTYVGTIIHVFRYP
                     QDILCTHCKGELHNKPVVGMQFMYDMEYKKGELVNGKIIDVKTGKTYYTKLSYNKQDD
                     ALLMRASVDASGFIGETQVWKRYK"
     gene            1033..>2357
                     /locus_tag="PLJ82_08170"
     CDS             1033..>2357
                     /locus_tag="PLJ82_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41516.1"
                     /translation="MKFFTIFICSIMYCVSVLADASISIATLRQSNDNNPYLQSEVVV
                     EGVVTGVFQQSNQIGGFFIQDEKGIFVEHTTKKVNIGDSVRITATLTNNNNRLQLTNV
                     SALDIVNSNNQIPITKLIFPDDFDSLEAWEGTLVELDQTMYVTNNYNLFRYGQLTLSA
                     NRLYIPTHQALPLSDVYFQTISSNEINQLILDDASSVSYPTNKPFTDSNGTRRMGEKI
                     DNLQVIIDQVGTNYVVYAPKDIDFYGNPRTATHPSIGDYSIKVCGTNLEMFKTSYWGD
                     GFGADNSTEFARQRTKILAALHAINADIYAITEIQQGSNALENLVNGLNALRGNTDFT
                     YINDKDNVSEYIKSAFIYNKNKVTPHLNVINNNTISSTRFRKKAQAFIENSSKEKFIV
                     VVNHYKAKSGCPSSGADADKQDGQGCYNATRTQEAVQSLTFINYMINYA"
BASE COUNT          787 a          409 c          393 g          768 t
ORIGIN      
        1 caacctcaga tatttggttg gctcgatttg ctagatatga acgtgtgggt aattttaata
       61 ctgatgctta cggtggctgg ttttagtatg atatcgggct tgttaattct aatcttggaa
      121 cgtaccaata tgattggcat attgaaagct ttgggaggta aaaactggtt tattcgtaaa
      181 atatttttat atcaagcgtt cttcttagta ggtaaaggca tgctttgggg aaatatattg
      241 gggattagtt tggctttgct acaacaacaa ttcaatatca ttaccctcga tcctcttact
      301 tattatgtaa ataccgtccc tatcaatctc aatctgtggt acatagtatt cgtgaatgct
      361 ctttgcttat tcttttctct tttggtgtta ttagctccat cgcatatgat tgccaaaatt
      421 gagccatcaa aagctattcg gttcgaataa tgaatatgaa gactatttat agcgtttcca
      481 aacctgtgtt tcgccaataa aaccagatgc atcgaccgaa gctctcatta gcagagcatc
      541 atcttgttta ttgtaagata gtttagtgta atatgttttt cctgttttta catcaataat
      601 cttaccattt acgagctctc cttttttata ttccatatcg tacataaatt gcatgcctac
      661 aactggtttg ttatgcaatt cgcctttaca atgggtacat aaaatatctt gtggatagcg
      721 aaaaacatga ataatagttc caacataggt gtttgctcga gatgattttt ctatacgcac
      781 caaagctcct ttttgtccat cattatttat ttgttcccaa acaccaagat aagattgagc
      841 catagttgat acactgcata gtgtaaacac gaaaaggaca tatattttgt ttaaacctac
      901 aaatggatac gacatacact actagttaga aattttacta gttacaaaac tataaattat
      961 taagaacatt atcaatttct actcaaaaaa agtatctttg tagttcgcat ttttttatta
     1021 attatcattt taatgaaatt tttcacaatc tttatttgtt ctatcatgta ttgcgtttca
     1081 gtcttggctg atgcaagtat atccattgct acattaagac aatcgaatga taataatccc
     1141 tatttacaat ctgaagtagt ggtggaaggg gttgttacgg gtgtgtttca acaaagtaat
     1201 caaattggag gtttttttat acaagatgaa aaaggaattt ttgttgaaca taccacaaaa
     1261 aaagtaaaca ttggagatag tgtacgtata actgccacac tcactaacaa caataacaga
     1321 ttacaattaa cgaatgtatc tgctttagat attgtaaact caaacaacca aatacccatt
     1381 acaaaattaa tttttcctga tgattttgat tcactggaag catgggaagg aactctcgtt
     1441 gaattagacc aaaccatgta tgttaccaac aactacaacc tttttcgata cggacagctt
     1501 accttatctg ctaaccgttt atacattcca acgcatcaag cactcccatt aagtgatgtg
     1561 tattttcaaa ctatttcgag taacgaaatt aaccaactaa tactagatga tgcatcttct
     1621 gtatcatatc ctacaaataa gccttttaca gatagcaatg gcacacgacg aatgggtgaa
     1681 aaaattgata acttgcaagt cattatcgac caagttggaa caaattatgt agtgtatgct
     1741 ccgaaagaca ttgattttta tggcaatcca cgtacagcaa cgcatccatc aattggagat
     1801 tattcaataa aagtttgtgg cacaaattta gaaatgttta aaacgtctta ttggggagat
     1861 gggtttggag ctgataatag taccgagttt gcacgccaac gaacaaaaat tttagcagct
     1921 ttgcatgcaa taaatgcaga tatttatgcc ataacagaaa ttcagcaagg gtcaaacgca
     1981 ttggaaaacc tagtgaatgg acttaatgca ctacgtggaa acactgattt tacctatatt
     2041 aatgataagg acaatgtgtc tgaatacata aaatctgctt ttatttataa caaaaataag
     2101 gtaacaccac acttaaatgt aatcaataac aatacaatat caagtacccg ttttagaaaa
     2161 aaagcacaag cttttatcga aaacagctcc aaagaaaaat ttatcgttgt tgttaatcac
     2221 tataaagcca aaagcggatg cccatcatcg ggggcagatg ctgataagca agacggtcaa
     2281 gggtgctaca atgctactcg tacacaagaa gcggttcagt ctttaacgtt tatcaattat
     2341 atgattaatt atgccaa
//
LOCUS       DAOSRP010000166         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_43284_length_2275_cov_3.591892, whole genome shotgun sequence.
ACCESSION   DAOSRP010000166 DAOSRP010000000
VERSION     DAOSRP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43284_length_2275_cov_3.591892"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2207
                     /locus_tag="PLJ82_08175"
     CDS             <1..2207
                     /locus_tag="PLJ82_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOR41517.1"
                     /translation="KQFLVVILAFFSIGVGTNYAQIQSGGFSPLPFTYSNEQNTIVGK
                     RNTQPLFYKIPYNGEQDNKEEKTSRFVVGKSFAVDYSPLNAGQQFTTEDGTTIWRIGF
                     YSPNAASISVVFDEFEIPKGASIFVYNPSQTNVAGAFTSINNNSNKSLPIAPFASDSI
                     IIEYQQPATVTFNGKLRIGRVTHNAIDFINRSTGSFNSAEKCSPHAAFVEGVSNIKQA
                     TCLLYVTDNSESWLGSGVMINNSEGKPYLLTAAHNYEGTNLEQTTIFYFNYEVPKVDS
                     LIRGSQEFTLGGSVLKAKAYDIDFALVELNQMPPADYRPYLAGWNRASSDLAPMVCVQ
                     HPQGDVKRVSTDNDNPLAVTYPYNDLLETKLADAHWKITRWEKGTTEPGSSGSGLFDN
                     NYRIIGGLTGGNSTCNSPVEDYFFRLNKAWSYYSSESKQLKRWLDPTNTNVMYMDGRN
                     PYEATPCIRYSNVQSADVMGKVSVGNGKTGRLSGHNELQYTEFAEKFVTDTNNYLYGV
                     YIVPFIGKYNVNTPVNVHVYNGTSSPETLLTTVLFNPKTLAYQATSQTFAQTTKTQFL
                     NRENYVRFPNPVLVDEEFFVSYSITYQPVDSFAVYMVNNRAIDALNTAYYKDNGIWKS
                     FTEHPYKPMASSLWIEPVFQEANESLIFYPHDEDANAILFPNPCDSEIQVFRKETNTA
                     QFSLFSIQGTLLFTISSIENPLTINTSGLQQGIYFLQITRDDTSNTYKLIKK"
BASE COUNT          769 a          444 c          414 g          648 t
ORIGIN      
        1 tgaaacagtt tttagtcgta atattggcgt ttttttctat tggcgttggc acaaactacg
       61 ctcagataca aagtggagga ttttcgccac taccttttac gtattcgaac gaacaaaata
      121 ccattgttgg aaagagaaat acgcagccgt tattttacaa aatcccctat aatggagaac
      181 aagacaataa ggaagaaaaa acatctcgct ttgttgttgg caaatcgttt gctgtagatt
      241 attctccgtt aaacgctgga caacaattta caaccgaaga tggcacaaca atctggcgta
      301 ttgggttcta ctctccaaat gcagcctcta ttagcgtagt atttgatgag tttgaaatac
      361 ccaaaggggc ttcgattttt gtttacaatc catctcaaac caatgtagct ggtgcattta
      421 ccagtataaa taacaacagt aacaagagcc ttcctattgc accttttgcg tctgatagta
      481 ttattataga ataccaacag cctgccacag ttacctttaa cggcaaacta cgtattggca
      541 gagtaacaca caatgcaata gattttatta accgaagtac tggatcgttt aatagtgctg
      601 agaaatgcag tccgcatgca gcatttgttg aaggagtaag caacatcaaa caagccacat
      661 gtttgctgta tgtaaccgac aattccgaat cgtggttagg ctctggcgta atgattaaca
      721 atagcgaggg gaaaccttat ttgcttacag cagcacataa ttacgaaggg actaatttag
      781 aacaaacaac cattttttat tttaattatg aggtgccaaa agttgactca ctcattcgtg
      841 gttcgcaaga atttacttta ggaggaagtg tattaaaagc gaaagcgtat gatattgatt
      901 ttgcactggt tgagctaaac caaatgccac cagcagatta ccgtccttat ttagctggat
      961 ggaatagagc aagctctgat ttagctccta tggtatgcgt acagcatcca caaggagatg
     1021 ttaaacgagt gtcaaccgac aatgacaatc ctcttgcagt tacttacccc tacaatgatt
     1081 tgctagaaac caaattagca gatgctcact ggaaaataac tagatgggaa aaaggaacga
     1141 cagaaccggg ctcttctggt tcgggtttgt ttgacaacaa ctaccgtatt attggcggac
     1201 taacaggagg taattcaacc tgtaatagtc ccgtagaaga ttatttcttt cgtttaaaca
     1261 aagcttggag ttattatagc agcgaaagta aacaattaaa acgctggtta gaccctacca
     1321 ataccaatgt tatgtatatg gatggaagaa atccatacga agcaacccct tgcatacgtt
     1381 attcaaacgt acaaagtgca gacgtaatgg gcaaggtctc tgtaggaaat ggtaaaactg
     1441 gacgtttgtc gggacataat gaactgcaat atacagaatt tgcagagaaa tttgtaacag
     1501 ataccaacaa ctatctatat ggcgtgtaca ttgtgccatt tattggcaaa tacaatgtga
     1561 atactcctgt aaacgtacat gtttataatg gaacatcaag ccccgaaaca ctattaacga
     1621 cagttctctt taaccctaaa acactagcat atcaagctac ttcgcaaaca tttgcacaaa
     1681 ctactaaaac tcaattttta aatagagaaa attatgtacg ctttccaaat cctgttttag
     1741 ttgatgaaga attttttgtt tcctattcca ttacttatca accagtagat tcgtttgctg
     1801 tgtatatggt aaataacaga gcgatagatg cactcaatac cgcctattac aaggacaatg
     1861 gaatatggaa atcgtttacc gaacatcctt acaaaccaat ggcaagttcg ctatggattg
     1921 aacctgtatt tcaagaagca aacgaaagtt taatctttta cccacacgat gaagacgcta
     1981 atgcaatttt atttcccaat ccatgcgatt ctgaaattca agtatttaga aaagagacaa
     2041 atacagccca attctctctg ttttctatac aaggaacact cctctttaca ataagtagca
     2101 tcgaaaatcc acttaccatc aatacgagtg ggttacaaca aggcatctac tttttgcaaa
     2161 taacaagaga cgatacatca aacacctata aattgataaa aaaatagcta tgcttgctaa
     2221 aacacgggct attgttattc acactacaaa gttcaacgat aaagatctta ttctg
//
LOCUS       DAOSRP010000167         2229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_44485_length_2229_cov_4.088776, whole genome shotgun sequence.
ACCESSION   DAOSRP010000167 DAOSRP010000000
VERSION     DAOSRP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44485_length_2229_cov_4.088776"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1338
                     /gene="obgE"
                     /locus_tag="PLJ82_08180"
     CDS             238..1338
                     /gene="obgE"
                     /locus_tag="PLJ82_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOR41518.1"
                     /translation="MSNSNFVDYVKIYCRSGKGGAGSMHLHREKFITKGGPDGGDGGR
                     GGHIILRANANYWTLLHLKYARHVFAGHGEKGSGSRSFGKDGEDQIIEVPCGTVAYDA
                     ETGEFVCDVTDHGQEVILLKGGRGGLGNWHFRSATNQTPRYAQPGEPMQEKTIILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIDNYAFTTLEPNLGIVSYHDHKSFVMADIP
                     GIIEGAHEGRGLGLRFLRHIERNSVLLFMVPADSADIHKEYRVLLNEIEQYNPELLDK
                     RRVLAISKSDMIDDELELAMKKDLPSIPCVFISSITGKGIAELKDKLWDEIMAEPIKD
                     IVHKHKFVLIADDEVVDDEEPFYEEDSEEDDE"
     gene            1427..1729
                     /locus_tag="PLJ82_08185"
     CDS             1427..1729
                     /locus_tag="PLJ82_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOR41519.1"
                     /translation="MNIFVGSLSFNVKDDQLKAAFENFGEVSSARVLTDKFTGRSKGF
                     GFVDMPDNAAAQKAIDALNGTELEGRAMVVNEARERTEDSRRPSTGGYQKRDNSGF"
     gene            1883..>2229
                     /locus_tag="PLJ82_08190"
     CDS             1883..>2229
                     /locus_tag="PLJ82_08190"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOR41520.1"
                     /translation="MPGTLVNLSSFLQQQLSQWDVAYTNYQALQSVKKRVIEAQTGKL
                     IVQYNPARIVSSAAKTDAKSIEARPCFLCATNRPQEQLQYSYHNTYDILVNPFPIFPE
                     HSTVPHKQHIPQQ"
BASE COUNT          752 a          334 c          453 g          690 t
ORIGIN      
        1 gaactatcaa aaaacgtatt caaacttacg aagatcaaac aatcccagtt atacagtttt
       61 atcacaaaca aggtaaacat gtgaagattc agggtattgg tactattgac gaaatttttg
      121 aacgtgtgtc tactgctatt gatgcggtaa aataatacat actatgttta tttatacaga
      181 tagtatcgat actatttcgg actatctgtt ttttttcttt ttatacaaaa ccaatctatg
      241 tctaattcaa attttgtaga ttacgtaaaa atctattgtc gttcgggtaa aggtggtgca
      301 ggttcgatgc acttacacag ggaaaaattt attaccaaag gaggacctga tggaggtgat
      361 ggaggtagag gggggcatat tatattaaga gcgaatgcca attattggac tttgttgcac
      421 ttaaaatatg ctcgtcacgt atttgctgga cacggcgaaa aaggcagtgg tagtcgaagt
      481 tttggtaaag atggtgaaga ccaaataatt gaagttcctt gtggcactgt agcttatgat
      541 gccgaaacgg gagaatttgt atgtgatgta actgaccatg gtcaagaagt cattttgctc
      601 aaaggaggaa gaggaggttt aggtaattgg cattttcgct cggcaacgaa tcaaactcct
      661 cgttacgctc aaccaggaga gccaatgcag gaaaaaacca ttattttgga acttaaaatt
      721 cttgctgatg tagggctagt tggtttcccg aatgcaggaa aatctacctt actttcagta
      781 gtatccgcag ccaaacccaa aattgataat tatgctttta caactttaga accgaattta
      841 ggcattgtaa gttatcatga ccataaatcg tttgtcatgg cagatattcc tggtattatt
      901 gaaggagcac acgagggtag aggtttaggt ttacgatttt tacgccatat cgaacgtaat
      961 tccgtgttat tatttatggt tccagctgat agtgcggata ttcataaaga ataccgtgta
     1021 ttacttaatg aaatagaaca atataatcct gaactattag acaaacgtag ggtattggca
     1081 attagtaagt cagatatgat tgatgatgaa ttagagttgg cgatgaaaaa ggacttacca
     1141 agcattcctt gtgtatttat atcttcaatt actggaaaag gtattgctga attgaaagat
     1201 aaattgtggg atgaaattat ggctgaacca attaaagaca ttgtacacaa acataaattt
     1261 gtcttaatag ctgatgatga ggtggttgat gatgaagaac ctttctacga agaagatagc
     1321 gaggaagatg atgaataagc ttaaaacaat cttatttttt agataatagt ttgtaaacaa
     1381 gaaaaataca ttatctttgt cgaattattt ttaatacata taacgaatga acatttttgt
     1441 aggaagtcta tcttttaacg taaaagatga ccaattgaaa gcagcctttg agaattttgg
     1501 agaagtatct tcagcacgag ttttaactga taaatttact ggaagaagta aaggttttgg
     1561 atttgttgat atgccggata atgcagcagc acaaaaagct atcgacgcat taaatggtac
     1621 agaactagaa ggtcgtgcaa tggtagtaaa cgaggctaga gaaagaactg aggactctcg
     1681 cagaccaagt actggtggtt accaaaagag agataacagc ggtttttaaa agagatatta
     1741 ataaggtgta aatctttatt atgagaaaaa gagcgtttta ttatttaaaa tgctcttttt
     1801 gtttgtaatt agctttgttc aaaggataat ttacaaagaa aaagattgta tttttgtgtt
     1861 ttgatgtaat acaacttgaa acatgccagg tacactagtt aatttatcct cttttttaca
     1921 gcaacaactt tctcagtggg atgtagcata tacgaattac caagccttgc aatcggttaa
     1981 aaaacgtgta attgaggcac aaacaggtaa gttgattgtt cagtataatc cagcacgtat
     2041 tgtatcatcg gcggctaaaa ctgatgccaa aagtatagaa gcacgccctt gctttttgtg
     2101 tgctaccaat agacctcaag agcaattgca atattcgtat cataatacat acgatatatt
     2161 ggttaaccca tttccaattt tccctgaaca ttctactgtc cctcacaaac aacatattcc
     2221 acagcaaat
//
LOCUS       DAOSRP010000168         2228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_44526_length_2228_cov_3.823286, whole genome shotgun sequence.
ACCESSION   DAOSRP010000168 DAOSRP010000000
VERSION     DAOSRP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44526_length_2228_cov_3.823286"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLJ82_08195"
     CDS             complement(<1..432)
                     /locus_tag="PLJ82_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR41521.1"
                     /translation="MNNKIRVIADCHIPFLQGTIDNIATVTYLAASEISNRTVKDADA
                     LLIRTRTHCNESLLNSSKVKFIGTATIGYDHIDTQYCLQKGIVWKNAPGCNAASVAQY
                     IVAALLYMARKHSLSLTESTIGIIGVGHVGKQVAKMAETLGM"
     gene            complement(425..>2228)
                     /locus_tag="PLJ82_08200"
     CDS             complement(425..>2228)
                     /locus_tag="PLJ82_08200"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOR41522.1"
                     /translation="EEGFWASSYTREIPLHHLSVSIVKTTENVTNRILANIPEFETQE
                     KKQHLIDSISQLLIKEKQATTSYIVEFKASNKGTQFHLYTYEKFNDIRLVYAPPTSIA
                     LFGEEKDKHSTPKHNANFAVLRVYVSQENQPAPYDPYNKPYNTKQYAKISTRGYEMDD
                     FVVTVGYPSFTNRQITSFALKEQVYAINQAELNVALVKDKVWEHAMRESNYVKNLYEK
                     KYTQNNERLHYLIQLQESVKKQNIIAEKERTEKNFMQWSIKSDFNTMLKYEHVVPTLE
                     NKYTSRKQEMYHVHFLTESFVKLDILTVAYMLLTISPQNEREIYSEIAKFYERYDEQT
                     EKKMLIAMLELYANEATNEFMPDIYTTINSKKYKGNIEKYVNDTFKKSFITNEVRFNK
                     YLDKPSDKQFNNDPIILLSKSILKKRAEIARIAFADEKEIEHLTALLIEGYQRKNKEN
                     TLLADANYTLRMSYGTITTYSPSDGVFFGYTSNFEGIIEKNNTRHAAYKIDKRLVDIY
                     QSKHNKNEKPFAVSFLTSIDYTIDKMGAPVFDAEGNLIGILSDGNNETISNIYHYNER
                     FQRTIALDIRYMLYILEHYAGAKRVIEKLNLVDE"
BASE COUNT          586 a          364 c          421 g          857 t
ORIGIN      
        1 cataccaaga gtttctgcca tttttgctac ttgcttgccc acatgtccta ctccaataat
       61 gccaatagta ctttctgtaa gcgatagaga atgtttgcgt gccatataca gcaatgcagc
      121 aacgatatat tgagctaccg aagcagcgtt gcaccctgga gcatttttcc atacaatccc
      181 tttttgaagg cagtattggg tatcgatatg gtcgtagcca atggtagcag tgccaataaa
      241 ttttacctta ctagagttta aaagcgattc gttgcagtga gtgcgtgtgc gaataagtag
      301 tgcatcggca tctttcactg ttctattgct tatttcactc gcagctaagt aagtaacagt
      361 agcaatattg tcaatagtcc cctgtaaaaa tgggatatga cagtcagcta ttacacgtat
      421 tttgttattc atcaacgagg tttaattttt caatcacacg ttttgctcca gcataatgtt
      481 caagaatata caacatatat cgtatatcaa gggcaatagt tcgctgaaaa cgttcgttgt
      541 agtggtatat attgctgatg gtttcgttat ttccatcact tagtatgcca attaaatttc
      601 cttctgcatc aaatacggga gctcccattt tatctatagt ataatcaata gatgtaagaa
      661 aggataccgc aaaaggtttt tcattcttat tgtgtttgct ttggtaaata tctactaagc
      721 gtttgtctat cttataggcg gcgtggcgtg tattattttt ttcgataatt ccttcaaaat
      781 tagatgtata gccaaaaaat actccgtctg aggggctata cgtagtaatg gtgccataac
      841 tcatacgtaa ggtgtaatta gcatccgcta acaaggtgtt ttctttgttt tttcgttggt
      901 agccttctat taataatgcg gttaaatgtt caatttcttt ttcgtcagca aaagcaatac
      961 gggctatttc ggcacgtttt tttaatatac ttttgctcaa tagaatgatt gggtcgttgt
     1021 taaattgctt gtcagagggt ttgtcaaggt atttgttgaa gcgtacttcg ttggtaataa
     1081 atgatttttt aaatgtatcg tttacgtatt tttcaatatt tcctttgtat tttttactat
     1141 taatagtagt atatatgtcg ggcataaact cgtttgttgc ttcgttggca tagagttcga
     1201 gcatggcaat taacatcttt ttctcggttt gttcatcata acgttcatag aactttgcta
     1261 tttcgctgta tatttccctt tcgttttgag gagaaatcgt caataacata taggctactg
     1321 taagtatatc aagcttaaca aaagattctg ttaagaaatg gacatggtac atttcttgtt
     1381 tgcggcttgt atatttattt tctaacgtag gtactacgtg ttcatattta agcatggtgt
     1441 taaaatcgga ttttatggac cactgcataa aattcttttc tgtacgttct ttttcagcta
     1501 tgatgttttg tttttttact gattcttgta attggattaa ataatgtagt cgttcgttgt
     1561 tttgagtata ttttttttcg tacaagtttt ttacataatt actttctctc atagcatgtt
     1621 cccatacttt atctttcact agggcaacat tcagttcggc ttgattgata gcatacacct
     1681 gttctttcag tgcaaatgat gtaatctgtc tgttggtaaa acttggatat cctaccgtta
     1741 caacaaaatc atccatttcg tatccacgtg tgcttatttt tgcatattgt ttggtattgt
     1801 aaggcttgtt atatgggtcg tatggggctg gttgattttc ttgcgaaaca tacactctga
     1861 gtactgcaaa atttgcattg tgttttgggg tagagtgttt atctttttcc tccccaaata
     1921 gtgcaatgga ggtaggaggg gcatatacca agcgaatatc attaaatttt tcgtatgtgt
     1981 ataaatggaa ttgggtacct ttattacttg ctttaaactc aacaatatat gaggttgtag
     2041 cttgtttttc ttttattaat agttgactta tagaatctat caagtgttgt tttttttctt
     2101 gagtttcaaa ttctggaatg ttggcaagaa ttctattggt aacgttttct gttgttttta
     2161 ctatgctcac agatagatga tgcaatggaa tttcgcgtgt ataagagctt gcccaaaaac
     2221 cttcttct
//
LOCUS       DAOSRP010000169         2130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_47367_length_2130_cov_4.781205, whole genome shotgun sequence.
ACCESSION   DAOSRP010000169 DAOSRP010000000
VERSION     DAOSRP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47367_length_2130_cov_4.781205"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..2035)
                     /locus_tag="PLJ82_08205"
     CDS             complement(170..2035)
                     /locus_tag="PLJ82_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOR41523.1"
                     /translation="MKTHFHLLLFFFVCLHFGVHAENAKQGVSVWKIDPHSGDKEFIE
                     VDTIALNYHSKTNPVYNHTIATSWLGNLGLPAESKIYFDKNQGYVTHSFLFKNTYASY
                     FLSPENISFYNTKTPFSNVSYHTGGFTYREEDNISGLFTVNATPKLNFSLLANHIYGR
                     GAYQNQATDGLSGALYGSYLGDKYGIHFITALNNLKHFENGGIADISQLTSTIESYNL
                     PVNLSNAWSIFHSFYLYASQQYHLGFHTNDNKFVPVTTFGHSISFEQNQKKYYEKSIS
                     DFYQNSYYSTTSTNDTVAFNVLKNTAYISLNEGFHKWALMGVRAFIDFDIEQNTDLTA
                     DWSLRKKTTSLVYAGGDISKTKGNTTYHALGKMMLLGDARGEYELNGKLNTQLKLGSQ
                     NIQLQGKALLVSSNPYHHLEHYYSNHFMWERDYENIVRTQCSGSISLPNKVANITLKA
                     GVENTTNYIYFDTNALPQQYSNAIQIVHGEATLNLHLGNIHFENTAIFQQSSNEAILP
                     LPSLSSYHNLYVTHTLLNVLKFQIGADCRYHTAYYANAYMPATGQFYRQTETLIGNYP
                     FINLYANFHLKRTRFFVMYYNLSEAIFTDKNYTLVPNYPLNPGMVKFGLSWNFYD"
BASE COUNT          654 a          322 c          408 g          746 t
ORIGIN      
        1 atgctatgat atctttatag tctgttgaga atagcggaga tgagtgtcgc aagaaagcag
       61 aaagtactgt aaaagaaata actagaatga cgaataccaa tgatattttt gaacataatt
      121 tcataggaca tttgtttttt taagtaagtt atacacaaag atactcaaat taatcataaa
      181 aattccacga taagccaaat ttaaccattc cagggtttaa tgggtaatta ggtactaagg
      241 tgtaattttt gtccgtaaaa attgcttccg aaagattgta gtacattaca aaaaagcgtg
      301 ttcgtttgag atgaaagttg gcatataggt ttataaatgg gtagttacct ataagggtct
      361 ctgtttggcg gtaaaattgc ccagtagcag gcatatatgc attggcataa taagctgtgt
      421 gataacgaca atctgctcct atttgaaatt tgagtacgtt gagtaatgta tgcgtaacat
      481 acagattatg ataagaagat aggcttggta agggtaaaat agcctcattt gaactttgtt
      541 gaaaaatagc agtattttcg aaatgaatat tgcctagatg caggtttagt gttgcctcgc
      601 catgcacaat ttgtatggca ttgctgtatt gttgaggcaa agcattggtg tcaaaataga
      661 tgtaattagt ggtattttct actcccgctt tgagtgttat atttgctact ttgtttggca
      721 aactaatgct accactacat tgggtgcgaa caatattttc gtaatccctt tcccacataa
      781 aatgattgga ataatagtgc tcaagatgat ggtacggatt actgcttact aaaagagcct
      841 tgccttgtaa ctgtatgttt tgcgaaccca atttcaattg tgtattcaat ttgccattta
      901 attcatattc tccacgtgca tcgcctaata gcatcatttt gcctaaagca tgataggttg
      961 tgttgccttt tgtttttgaa atgtctcctc ctgcatatac taggctcgta gtttttttgc
     1021 gtaaactcca atcagctgtt aagtcagtat tttgttctat atcgaaatca ataaaagcac
     1081 gtactcccat taatgcccac ttatgaaaac cctcatttag agatatatat gctgtatttt
     1141 ttaatacatt gaaagccaca gtgtcgttgg tgctagtggt actgtagtat gagttttggt
     1201 aaaaatcaga tatgcttttt tcgtagtatt tcttttggtt ttgttcaaac gatatactat
     1261 gaccaaaggt agtaacagga acaaatttgt tgtcgttagt gtgaaatcca agatggtatt
     1321 gctgtgatgc atacaaataa aatgaatgga aaatagacca tgcattggat aaattgacag
     1381 gtagattata actttctatc gtactcgtta gttggcttat gtcagcaata cctccatttt
     1441 caaaatgttt taggttgtta agtgctgtga taaaatgtat gccatatttg tctcctaaat
     1501 agctaccata caatgctccg ctaagtccgt ctgttgcttg gttttgatag gctcctcgtc
     1561 cgtatatatg gttggctagt aacgaaaaat taagtttagg tgtagcgttt actgtaaata
     1621 atcccgaaat attgtcttct tctctgtaag taaaacctcc tgtatgatag cttacattac
     1681 taaaaggtgt ttttgtattg taaaatgaaa tattttcggg cgaaaggaaa taggatgcat
     1741 acgtattttt aaacaaaaat gaatgggtta cgtagccttg atttttgtca aaataaattt
     1801 ttgattcagc aggtagtcct aagtttccta gccacgatgt tgctatagtg tggttatata
     1861 ctggatttgt ttttgaatga taattaagtg ctattgtgtc tacctctatg aattctttat
     1921 ctccactatg tggatcaatt ttccaaacag aaactccttg ttttgcattt tctgcatgca
     1981 ctccaaaatg caagcacacg aaaaaaaaca aaagaagatg gaagtgtgtt ttcatgcaat
     2041 aaataggtat tattgatcaa tgttaaagat agcttggtat ctgtgtgttt aatcctctga
     2101 aaatatactc tttttatagt gtcaaaagta
//
LOCUS       DAOSRP010000170         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_49299_length_2071_cov_6.479167, whole genome shotgun sequence.
ACCESSION   DAOSRP010000170 DAOSRP010000000
VERSION     DAOSRP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49299_length_2071_cov_6.479167"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2071)
                     /locus_tag="PLJ82_08210"
     CDS             complement(<1..>2071)
                     /locus_tag="PLJ82_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOR41524.1"
                     /translation="PMYLSAYSGVETDKLSVTLLNRDTQQSSLQVKLRMRIYCSQGQE
                     ISSRAESLYTPITLEGGIPLRLSMSDLAAYFRTENLRIAGSLPQGKLPVGHTRFCFEV
                     LEYYTGRLLSSQGCGSAFIVLQTPPLLQQPSHGSLLTDDPTGGILFRWSPQHSVGLER
                     VQYEFILKEMFDTQQSAELGFAHGVEVYRTHTTATSLLYGMMQPPLIAGKRYVWQVRA
                     FTDPISGTPLAFENNGQSRVFTFDIDYPCQAPQHIIATAHTNGIHLTWDASPHPSASY
                     HLSYRRKDFSSTWHQAVTTANSYTISNLTPGANYEYKVGISCTNKTNDIHYSTVAHIA
                     YNPNESETPPTSIQTKVKECGKSRQNNISNTEPKETLWAGDVIYAGDFTITLSTVQGS
                     NGRFTGEGKVHVPYLSGIGMSVYFSDISINTDNQLIEGKIHTVYDESRGTIMNLDAFD
                     HGGTTTPAQGVLVPNYEFDFALTNVTRIHNDPLTNSLIFYDATGSRVGNYELTGTAPS
                     FPVLVSDKEGNMYSINETNKDNPPANYTLVAVGKQIGELAGNAFDRNVVDSHKGTVTF
                     FDAGVGTYSFDEFLPQYDNINYIRYSSENGNERLYPQLGDNYYVPWKFVPLNTHDKIQ
                     AQISLDPNSELQPERVVFITESGTQVPASYNASNWTYQVSVTGTSNSGVHTLYALYPK
                     GDGTSYFH"
BASE COUNT          447 a          446 c          534 g          644 t
ORIGIN      
        1 aatggaaata acttgtgcca tcgcctttcg ggtacaaggc atacagcgta tgtacaccac
       61 tgttggatgt gcctgttacg cttacttggt aggtccagtt gctcgcatta tagcttgctg
      121 gtacttgcgt gcccgattcg gttataaaca caactcgctc gggttgtaat tcgctattcg
      181 ggtctaagct tatctgggct tgtatcttgt cgtgggtatt gagtggaaca aacttccacg
      241 gcacataata gttgtctccg agttgtgggt acagtctttc gttaccattt tcggaagaat
      301 agcgtatgta atttatattg tcgtactgcg gtaaaaactc atcgaaacta tacgttccta
      361 cacctgcatc gaaaaatgtg acagtgcctt tgtggctatc cactacattg cggtcgaatg
      421 cattgcctgc taactcgcct atttgtttgc ctaccgccac taaggtatag tttgctgggg
      481 ggttatcttt gttcgtttcg ttaatgctat acatattccc ctctttgtcg cttaccagta
      541 ctgggaagct tggggctgta ccagttagtt cgtagttgcc tacgcgtgag cctgtggcat
      601 cgtagaagat taggctattg gtaagggggt cgttgtggat acgggtaaca ttggtcagtg
      661 caaaatcgaa ctcgtagttt ggcactaata cgccttgtgc tggtgtggta gtaccgccat
      721 ggtcgaaggc atcgaggttc ataatagtac ctctggactc atcgtacacg gtatgaatct
      781 taccttctat cagttggtta tcggtattga tgctaatatc gctaaaatac accgacatgc
      841 ctatgcctga taaataaggt acatgtactt ttccttcgcc tgtaaagcgt ccgttactgc
      901 cttgtacggt gcttagggta atggtaaaat cgcctgcata aatcacatcg cctgcccata
      961 aggtttcttt gggttcggta ttgctgatgt tgttttgcct gcttttgcca cattctttta
     1021 ccttggtctg tatgcttgtt gggggcgttt cgctctcgtt ggggttgtag gctatatggg
     1081 ctactgtgct gtagtgtatg tcgttggttt tgttggtaca acttatcccc actttgtatt
     1141 cgtaattagc tcctggggtc aggttgctaa tggtatagct attggcggta gtaactgcct
     1201 ggtgccatgt gctggaaaaa tctttccgtc tgtagctcag gtggtagctc gctgatgggt
     1261 gtgggctagc atcccatgta aggtgaatgc cattggtgtg ggctgtagcg atgatgtgtt
     1321 gtggggcttg gcatgggtaa tcgatatcga aggtaaacac acgactctgt ccgttattct
     1381 caaaggctaa gggggttccg ctgattgggt cggtaaaggc tcttacctgc cacacatagc
     1441 gtttgcctgc tatcaagggc ggttgcatca tgccatacag taagctggta gcagtggtgt
     1501 gggtacggta cacttctacg ccatgggcaa atccaagttc ggcactctgt tgggtatcga
     1561 acatttcttt taggataaat tcgtactgta ctcgttctag tcctacgctg tgttgcggac
     1621 tccaacgaaa caagatgcct cctgtggggt cgtctgtcaa taggctgcca tggcttggtt
     1681 gctggagcaa tggtggggtc tgtagtacaa taaaggcact cccacagcct tgactcgaca
     1741 gtaacctgcc agtatagtat tctaacactt caaaacaaaa gcgggtatgc cctactggga
     1801 gtttgccttg tggtaagcta cctgctatac gcaggttttc tgttctgaaa taggctgcca
     1861 aatcggacat gcttaaccgc aacgggatgc ctccctctaa ggtgataggg gtatagaggc
     1921 tctcggctcg gctactgatt tcttgtccct gagaacagta aatacgcata cgcaacttga
     1981 cttgcaaact gctctgttgc gtatctctat tgagcaaggt aacggatagt ttatcggtct
     2041 ctactccact gtatgcggat aaatacatcg g
//
LOCUS       DAOSRP010000171         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_50203_length_2045_cov_4.917085, whole genome shotgun sequence.
ACCESSION   DAOSRP010000171 DAOSRP010000000
VERSION     DAOSRP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50203_length_2045_cov_4.917085"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLJ82_08215"
     CDS             complement(<1..1166)
                     /locus_tag="PLJ82_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOR41525.1"
                     /translation="MFKVFTKKMLGDIQTPVSIYLRVRDLYSQSALLESSDYHGGENS
                     FSFIAFNPLASFEVANNTMKASCIGNETIEKNIVDKFSVPTFFNEFVQSFSIAYQDEK
                     PVVNGFFGYTSFESVRYFDDIRVANKPLHEYDMPDMHYIFYRYIIAVNHFKNELLLIE
                     NVPDGEQSTLETIETLLYNLNLSTYSFKGIGAETSDITDAEYKAMVTEGKKACFRGDV
                     FQIVLSRRFSQAYEGDDFTLYRHLRSINPSPYLFYFDYGSFRIFGSSPEAHLVVNESK
                     AYINPIAGTFKRTGDDAKDLLLAEQLRMDPKENAEHVMLVDLARNDLSRNTHHVKVDI
                     FREVQYFSHVLHLVSQVSGEILPDTNVIQLFSDTFPAGTLSGAPKVRAIEIINQ"
     gene            complement(1191..>2045)
                     /gene="trpB"
                     /locus_tag="PLJ82_08220"
     CDS             complement(1191..>2045)
                     /gene="trpB"
                     /locus_tag="PLJ82_08220"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR41526.1"
                     /translation="AETGAGQHGVATATVCALMNMECVVYMGETDVKRQYLNVQRMYM
                     LGAKVVPVTSGNKTLKDATNEAIRDWTCNPKDTFYIIGSVVGPHPYPDMVARFQAVIS
                     EEIMWQLQQHENTSYPDYVIACVGGGSNAAGSFYHFLEHDSVKLIAAEAAGLGVDTNE
                     SAATIHLGKEGILHGSKTLIMQTDDGQIVEPYSISAGLDYPGIGPMHAFLAESGRAQV
                     YAITDDEALQAALQLTRLEGIIPAIESSHALAVLAKHSFKPNDVIVLTVSGRGDKDME
                     TYCNRLTF"
BASE COUNT          622 a          443 c          349 g          631 t
ORIGIN      
        1 aactgattga ttatttcgat ggctcgcacc ttgggtgcac ccgatagtgt gccagctgga
       61 aaggtatctg agaatagctg aataacattt gtatctggca aaatttcgcc acttacttga
      121 gatacaagat gtaatacatg cgaaaaatat tgcacctctc taaatatatc tacctttacg
      181 tggtgtgtgt ttctactcaa atcattacgt gccaaatcta ctaacatcac atgctcggca
      241 ttctctttcg gatccatacg caattgctct gctaataaca aatctttggc atcatcgcca
      301 gttcgcttaa atgtacctgc aataggattg atgtatgctt ttgattcatt tacaaccaag
      361 tgagcttcgg gcgaagaccc aaaaatacga aatgagccat aatcaaaata aaacaaatac
      421 ggagatggat tgatagaacg caaatgccta tatagggtaa aatcatcgcc ttcgtaggct
      481 tgcgaaaaac gccttgataa tacaatttgg aaaacatcgc ctctaaaaca ggctttttta
      541 ccttctgtaa ccattgcctt atattctgca tccgtaatgt cagacgtttc tgccccaatg
      601 cctttaaaag agtatgtaga taagttgaga ttatacaaca acgtttcaat ggtttcaagc
      661 gtagattgct ctccatcagg cacattttct atcaagagca actcattttt aaaatgattg
      721 acagcaataa tataccgata aaagatatag tgcatatctg gcatatcgta ttcgtgtagt
      781 ggtttgttag caacacgaat atcatcaaaa taacgaacag attcaaagct tgtataacca
      841 aaaaatccat ttacgacagg tttttcatct tgatatgcaa tggaaaatga ctgcacaaat
      901 tcattaaaaa atgtgggtac agagaattta tccactatat ttttttcaat cgtttcgttg
      961 ccaatacagc ttgctttcat tgtattgttg gctacttcaa aagatgccaa gggattgaat
     1021 gctataaacg aaaagctatt ttcgccacca tggtagtcgg aactctctaa caatgctgat
     1081 tgtgaataca aatctcgcac ccttagatat atacttacag gagtttgtat atctcctagc
     1141 attttttttg tgaatacttt aaacattgta ttttaaaatt taataagtaa ttaaaacgtt
     1201 aatcgattgc aataggtttc catatcttta tcccctctac ccgatacggt aagcactatt
     1261 acatcatttg gtttaaagga atgttttgcc aatactgcca atgcatgaga actttcgatg
     1321 gcaggtataa ttccctccaa acgtgtaagt tgaagtgctg cttgcaatgc ctcgtcatcg
     1381 gttatggcat acacttgtgc tctccccgac tcggctaaaa acgcatgcat cggaccaata
     1441 cctgggtaat ctaatccagc cgaaatagaa taaggctcaa caatttgtcc atcatccgtt
     1501 tgcataatca aggttttact accatgcaaa ataccctctt taccaagatg gattgtagct
     1561 gctgattcat tggtatccac tcccaaacca gctgcttctg ctgcaattaa tttcaccgaa
     1621 tcatgctcca aaaaatgata aaaagaacct gcggcattgc tacctccacc cacacatgct
     1681 attacataat cggggtacga tgtattttcg tgttgttgta actgccacat tatttcttcg
     1741 ctaataaccg cctgaaatcg agctaccata tccgggtaag gatgaggtcc taccacagaa
     1801 ccaataatat aaaacgtatc tttcggattg catgtccaat cgcgaatagc ctcgttagta
     1861 gcatctttta aggttttatt acctgatgtt actggcacaa cttttgcacc taacatatac
     1921 attcgttgca cgttaaggta ctgacgtttt acatccgttt cgcccatata cactacgcac
     1981 tccatattca tcaacgcaca taccgttgct gttgctacac catgttgtcc tgcacctgtt
     2041 tcggc
//
LOCUS       DAOSRP010000172         2032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_50683_length_2032_cov_2.711179, whole genome shotgun sequence.
ACCESSION   DAOSRP010000172 DAOSRP010000000
VERSION     DAOSRP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50683_length_2032_cov_2.711179"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..375
                     /locus_tag="PLJ82_08225"
     CDS             64..375
                     /locus_tag="PLJ82_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOR41527.1"
                     /translation="MKIAVSSTGNLLESPMDSRFGRCKYIALYDTENSVLEFISNPNA
                     EADSGAGPATAQLIASKDVGMVVSGDFGGKVKHILDDLKIEMVTVDTTMKTVKEIIKN
                     L"
     gene            393..608
                     /locus_tag="PLJ82_08230"
     CDS             393..608
                     /locus_tag="PLJ82_08230"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOR41528.1"
                     /translation="MPNLNRKGPNGNGAMSGRGLGKCNVENKGKSDQEILSSRNATSF
                     SQVANASMRQRNGMGNGKCKSGGRGNK"
     gene            630..1511
                     /locus_tag="PLJ82_08235"
     CDS             630..1511
                     /locus_tag="PLJ82_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR41529.1"
                     /translation="MKIAIASGKGGTGKTFVSTNLFYASVYDGNQITLVDCDAEAPNT
                     TLFLKGVCESIEHVYSYFPSIDVDACAFCGKCVEACHFNAIFYLSQKKRIHVLNELCH
                     SCGACSYVCKHGAITEHAKSIGTIKRIAYRNTSPIAECRMEIGVASSVPIIKQAIRNV
                     DNDAMVLLDSPPGVSCPFIATVETADYVILVTEPTPFGLNDLRLSIQTLQQMGIAFGV
                     IINRDGIGNQKMENWLNEENIPILMKIPYDSLIAETYAEGKIVSEINSMYLSKFRELM
                     KILEENSTEQRVALCKK"
     gene            1499..>2032
                     /locus_tag="PLJ82_08240"
     CDS             1499..>2032
                     /locus_tag="PLJ82_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR41530.1"
                     /translation="MQEIVVLSGKGGTGKTSITAALATIGKNLLAVDCDVDAANLYLL
                     LNPQNYREEKYTSGKTAFVHADVCTVCGKCVDACRFEAIRYFNDSIEVQETACEGCQL
                     CAAICPAHAIEMIPRDKSRWYEATYNNGYMVYARLFPGEENSGKLVSVIREKAKALAQ
                     KHQLDTILLDGPPGVGCP"
BASE COUNT          688 a          293 c          437 g          614 t
ORIGIN      
        1 caaatgttca ttatatttgc actgtaaata taaacatatg ttcataatta atacaaaatt
       61 tatatgaaaa tagccgtatc atcaacaggt aacttgttag aatcgccaat ggatagtcgt
      121 tttggacgtt gtaaatacat tgctctatac gatacagaaa attctgtact tgagtttatt
      181 tctaatccca atgcagaagc agatagtgga gcaggaccag caactgcaca attaatagcg
      241 tctaaggatg tagggatggt tgtgtctgga gattttggcg gtaaagtgaa gcatatatta
      301 gatgatttga aaattgaaat ggtaacagtt gataccacta tgaagactgt aaaagagata
      361 ataaaaaatt tataaaccta ataaagacaa ttatgccaaa tttaaacaga aaaggaccca
      421 atggtaatgg agcaatgagt ggaagaggct taggtaaatg caatgttgaa aataaaggaa
      481 aatcggatca agagattctt tcgtcaagaa atgcaacttc tttttctcaa gtagcaaatg
      541 catctatgcg tcaacgcaat ggcatgggaa atggaaaatg taagtctggt ggaagaggga
      601 ataaatagta ccaatcagac tttatttgta tgaaaatcgc tatagcaagt ggcaagggtg
      661 gaacaggtaa aacttttgtc tcgacaaact tattttatgc atcagtctat gatggaaatc
      721 aaataacttt ggtcgattgt gatgctgaag ctccaaatac aaccttgttt cttaaaggag
      781 tatgcgaatc aattgagcat gtttacagtt attttccttc tattgatgta gatgcgtgtg
      841 cattttgtgg aaaatgtgtt gaggcttgtc attttaatgc aattttttat ttatctcaaa
      901 aaaaacgtat ccacgtgctt aatgaattgt gtcacagttg cggagcatgt tcttatgtct
      961 gtaaacatgg agctataaca gaacacgcca aaagtattgg caccattaaa cgcattgctt
     1021 atcggaatac ttctcctatt gcagaatgtc gaatggaaat tggagtggct tcttcagtgc
     1081 caattattaa gcaagcaata cgtaatgtag ataatgatgc tatggtattg ttagattcac
     1141 ctccgggtgt gtcgtgtcca tttattgcca cagtcgaaac agctgattat gttatacttg
     1201 taacagaacc aactccgttt ggattaaatg atttacgttt gtctattcaa accttgcaac
     1261 aaatgggtat tgcatttggg gtaattataa atcgagatgg gattggtaac caaaaaatgg
     1321 aaaattggtt gaatgaagaa aacataccta ttttaatgaa aataccctat gattcgttga
     1381 ttgctgaaac gtatgctgaa ggtaagattg taagtgaaat taacagtatg tatctttcaa
     1441 aatttagaga attaatgaag atattggagg aaaatagtac agaacaaaga gtagccttat
     1501 gcaagaaata gttgtattaa gtggcaaggg tggtactggt aaaacttcca ttactgcagc
     1561 tttagctacc attggaaaaa atttattggc agtagattgt gatgtggatg cagctaattt
     1621 gtatttattg ctcaatcccc aaaattatag agaagagaaa tatacatcgg gaaaaacggc
     1681 ttttgtgcat gcagatgtat gtactgtatg tggcaagtgt gtagatgcgt gtcgttttga
     1741 agccatacga tactttaacg atagtattga ggtacaagaa acggcatgcg agggttgtca
     1801 gttatgtgct gctatatgcc ccgctcatgc tatagaaatg attccaagag ataaaagccg
     1861 ttggtatgaa gcaacgtata acaatggtta catggtatat gctcgcctat tccctggcga
     1921 agaaaattct ggaaaattag tgagtgtaat tagagaaaag gcaaaagcct tagctcagaa
     1981 acatcaacta gatacgattc ttttagatgg acctcctgga gtaggttgtc ct
//
LOCUS       DAOSRP010000173         2010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_51513_length_2010_cov_10.713555, whole genome shotgun
            sequence.
ACCESSION   DAOSRP010000173 DAOSRP010000000
VERSION     DAOSRP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51513_length_2010_cov_10.713555"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2010)
                     /locus_tag="PLJ82_08245"
     CDS             complement(<1..>2010)
                     /locus_tag="PLJ82_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41531.1"
                     /translation="AQRKEAEAKRSEMQEKMQPERRQMYQPERNAMPKATVRKKTTNN
                     IRQKEEVSKLALDSIVYPEFWKETYRYDYYGRTTEYIDYNWGSSDWEPSQKYEYVYEG
                     DIIREIRYNWNVSDWQPNSKYEYATDANDNQILNAVYNWDSVKWVGTDKYEYAYDAND
                     NQIMEAHYDWISDKWVGEYKYTIEYNNDIKITINYAWDTVKSQWQNDEKHEYAENDYG
                     WPLHNVRYTWDSTTNKWEGDYKYLHAYNSYGFSLLDERYDDWNDTTDTWIGSYKQVWA
                     YDDNGNDTLWEHYSAWNYTTNTWIGNAKHVTAYDDNGNNILHEYYTWNDTAGTWIGNN
                     KKVSAYDSDNNPLLEEFYNWSDSTGTWIKTDKYTYTYAHGMEFDKYYYVWNTDSNDWQ
                     EMEKTIGTIHTDGGISLRMVYQWNGSDWDTIEKWETMVYDTMGNMLETEVIFFTYTSP
                     IWDKQQKIVYTWDTNDKWTEKIIYNYNSGTWEEYEKYEQNHDIYGNQTMEADYSWNGS
                     AWVGNGYKCEYAYDTNGNRTMYADYLWSSDKWKGEDKFEWAYDANGNHTMEVDYNWND
                     SVWVENKKSEYDYDANGYQTLYAYYTWGDSVWVGYSKSEYGYDANGNQTMYAEYFWNS
                     GKWEGNYRDEWVYDANGKLLFGQFQYDWDSTWKYGDKYVYAYDIN"
BASE COUNT          500 a          449 c          309 g          752 t
ORIGIN      
        1 cattgatatc ataggcatat acatacttat ccccatattt ccaagtgcta tcccagtcat
       61 attgaaattg accgaataat agcttaccat tggcatcgta aacccattcg tctctataat
      121 ttccttccca tttaccactg ttccaaaaat attcggcata catggtttgg ttgccattgg
      181 catcatagcc atattcgctt ttagaatatc ctacccaaac actgtctccc caggtgtagt
      241 aggcatacaa ggtttgatag ccattggcat cgtagtcata ttcggatttt ttattttcta
      301 cccaaacact gtcgttccag ttgtagtcga cttccatagt atggttgcca ttggcatcgt
      361 aagcccattc gaatttatct tctcctttcc atttatcact gctccataaa taatcggcat
      421 acatggttcg gttgccattg gtatcatagg cgtattcgca cttataacca tttccaaccc
      481 aagcactacc gttccaagaa taatcggctt ccatggtttg gttgccgtaa atatcatgat
      541 tttgttcata tttctcatac tcttcccaag taccactatt gtaattatag ataatctttt
      601 cagtccattt atcattggtg tcccaagtat aaactatttt ttgttgttta tcccatattg
      661 gggaagtata agtaaaaaat atcacttcgg tttccaacat attacccata gtgtcgtaaa
      721 ccatggtttc ccatttttca attgtatccc aatcacttcc attccactga taaaccatac
      781 gcaaactaat acctccgtca gtatgaatgg tgcctattgt cttttccatc tcttgccaat
      841 cgttactatc cgtattccat acataatagt atttgtcaaa ctccattcca tgagcataag
      901 tataggtata tttgtcagtt tttatccatg tgccggtgct gtcactccag ttatagaatt
      961 cttctaataa tggattgtta tcgctatcgt aagcagatac cttcttatta tttcctatcc
     1021 aagtgcctgc agtatcgttc caagtatagt attcatgcaa tatgttattg ccgttatcat
     1081 cgtaagccgt tacatgtttt gcatttccta tccaagtatt tgtagtatag ttccaagcac
     1141 tatagtgttc ccacaatgta tcattgccat tatcatcgta agcccatact tgtttataac
     1201 ttcctatcca tgtgtccgta gtatcattcc aatcatcata acgttcatct aataatgaaa
     1261 aaccataact attgtaagca tgtagatatt tataatcccc ttcccattta ttagtggtgc
     1321 tgtcccaagt gtaacgaaca ttatgcaacg gccatccata atcattttcg gcatattcgt
     1381 gtttttcatc attttgccat tgtgatttaa cagtatccca agcgtaatta atggttattt
     1441 taatatcatt attgtattct atggtgtatt tatattctcc tacccacttg tcgctaatcc
     1501 aatcatagtg ggcttccatg atttggttat cattggcatc gtaggcgtat tcgtatttat
     1561 cagttcctac ccatttaaca ctatcccagt tatatacggc gttcaagatt tggttatcat
     1621 tggcatcagt agcgtattcg tatttgctgt taggctgcca atcgctaaca ttccaattat
     1681 atctaatctc tcttataatg tctccctcat aaacgtattc gtatttttgg cttggttccc
     1741 aatcgctact accccaattg taatcaatgt attcggttgt cctaccatag tagtcgtagc
     1801 ggtatgtttc tttccagaat tccggataca ctatgctgtc taatgcgagt ttgctaactt
     1861 cttctttttg tcggatgttg ttagttgttt ttttccttac ggtagctttt ggcattgcat
     1921 tcctttccgg ttgatacatt tgtcggcgtt caggttgcat tttttcttgc atctcggaac
     1981 gctttgcttc ggcttctttt cgctgtgctt
//
LOCUS       DAOSRP010000174         1845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_58308_length_1845_cov_4.988827, whole genome shotgun sequence.
ACCESSION   DAOSRP010000174 DAOSRP010000000
VERSION     DAOSRP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58308_length_1845_cov_4.988827"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1845
                     /locus_tag="PLJ82_08250"
     CDS             255..>1845
                     /locus_tag="PLJ82_08250"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOR41532.1"
                     /translation="MTKIKRVYSFGNKKAEGDAKMKELLGGKGANLAEMALMGLPVPA
                     GFTITTDTCTEYYNNNHELPEGLMDEVWAAMKKVEADMGLKYDDPDNALLVSCRSGAR
                     SSMPGMMDTVLNIGLSSNTIPGLIKKTNNPRFVYDSYRRLIMMYADVVMDKAEDLDKN
                     IRRQLDDMFEEYKVQRNYKSDTELTAEDLMIISDLFKKKIKAVLGTEFPDDAKKQLEG
                     SIKAVFKSWNGKKAVSYRRIEHIPDDWGTAVNVQAMVFGNMGETSATGVAFTRNPATG
                     ENLFYGEWLVNAQGEDVVAGIRTPNPLNNDTKNSQNEHLMSMQEQFPAIYKELDDIRT
                     TLEDTFKDMLDIEFTVQEGKLYMLQCRVGKRTGTAALNMAMEMLEEGRIDEKTAILRV
                     EPSQLDELLHPIVDPAAEKNHEPIVKGLPAGPGAATGKVVFTAEDAVEWTKRGEKVLL
                     VRKETNPEDVEGMRVADGLLTTLGGMTSHAALVARGWGKCCIVGAGNLEVREDEKIAI
                     VKGTDIVIKEGDILTLNGTTGN"
BASE COUNT          677 a          281 c          371 g          516 t
ORIGIN      
        1 aaaagaatta ttactaatgt acaacgccca agaaatatag aactttcaaa aaaaatacct
       61 atcatgtgtg taaatataag aactgacata caaaaactag gtggttttac ttattaatta
      121 agttttaact tttaacagca aattttatac ttttataaac atttaatatt ttaaatgttg
      181 taaaaaaaat atatatttgt gggcatgaaa aataaagaaa ttaattttaa taaaacttaa
      241 taacaaattt taaaatgaca aaaattaaaa gagtttacag ctttggaaat aaaaaagctg
      301 aaggagatgc aaaaatgaag gagcttcttg gaggtaaagg agcaaactta gccgaaatgg
      361 ctttaatggg gttaccagtt cctgctggtt ttactattac aacagacact tgcaccgaat
      421 attataacaa caatcacgaa cttcctgaag gattgatgga cgaggtgtgg gctgctatga
      481 aaaaagtaga agcagatatg ggattaaaat acgacgaccc tgataatgcc ctattggttt
      541 cttgccgttc tggtgcacgt agctcaatgc ctggtatgat ggatacagtt ttaaacatcg
      601 gtttaagtag caatacaatc cctggattaa tcaaaaaaac taacaatcca cgctttgttt
      661 acgattcgta tcgccgctta attatgatgt atgctgacgt tgtaatggat aaagcagaag
      721 accttgacaa aaatattcgt cgccaattag acgatatgtt tgaagaatat aaagttcaaa
      781 gaaattataa atcagataca gaattgactg ctgaagactt aatgattatt tctgatttat
      841 tcaagaaaaa aattaaagct gttttaggta ctgaattccc tgatgacgca aaaaaacaat
      901 tagaaggtag tattaaagca gttttcaaaa gctggaatgg caaaaaagct gtttcgtatc
      961 gtagaattga gcatattcca gatgattggg gtacagcggt taacgttcaa gctatggttt
     1021 ttggaaatat gggcgaaact tcggctacag gtgttgcatt tactagaaat ccagctaccg
     1081 gtgaaaactt attctatgga gaatggttag ttaatgctca aggagaagac gtagttgcag
     1141 gtattcgtac tccaaaccca cttaacaacg atactaaaaa ctctcaaaac gagcatttaa
     1201 tgtcaatgca agagcaattc ccagcaatat ataaagaact tgacgacatt agaactactc
     1261 ttgaagatac atttaaagac atgctcgata ttgagtttac agttcaagaa ggtaaacttt
     1321 atatgctaca atgccgtgta ggtaaaagaa ctggaacagc tgctctaaac atggcaatgg
     1381 aaatgttaga agaaggcaga attgatgaaa aaactgcaat tttacgcgtt gagccaagtc
     1441 aattagacga acttcttcac ccaatagttg accctgcggc agagaaaaac cacgaaccaa
     1501 tagttaaagg tttgcctgct ggaccaggtg cagctactgg aaaagtagta tttactgccg
     1561 aagatgctgt tgaatggaca aaaagaggag aaaaagttct tttagttcgt aaagaaacaa
     1621 atcctgaaga cgttgaaggt atgagagttg ctgacggact tttaactaca cttggtggaa
     1681 tgacttcaca cgcagctctt gttgcacgtg gctggggaaa atgctgtatt gttggtgctg
     1741 gtaacttaga agtaagagaa gacgaaaaaa tagcaatcgt taaaggaact gatattgtta
     1801 ttaaagaagg cgatatcctt actcttaatg gtactacagg taacg
//
LOCUS       DAOSRP010000175         1812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_59860_length_1812_cov_4.382470, whole genome shotgun sequence.
ACCESSION   DAOSRP010000175 DAOSRP010000000
VERSION     DAOSRP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59860_length_1812_cov_4.382470"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="PLJ82_08255"
     CDS             complement(<1..1046)
                     /locus_tag="PLJ82_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOR41533.1"
                     /translation="MAIKEILTTFIQNKKKKKVGENILALTLMNAVNLLSPLLLIPYL
                     IRVLGAEYYGVYIFAWTFISYFIMLVNYGYDLYATKQVAIHKNDKLYLSAIFYSITAS
                     RVLLAVVSSIIVVVCVWLIPEFNAHSILILYGIGVFVGQSLHPTWLFQGMEEMKFITI
                     ITLVTRLIPIVFIYIFVKGIADYYYITLFQSVGYLFGAALAFFVAMKRYQLIVKIPTY
                     SEILLQLKSSWTLFLSTLGITLYRETNVLILGFMTGNYTLVGYYALADKFLRLFQMMI
                     TPIGQALFPHFGNTFKENKELAFAQFHKIGRFFLLFLFSLAIFVFAFSPIMTEMYLGD
                     VFPSINKDIRVLSLV"
     gene            complement(1060..>1812)
                     /locus_tag="PLJ82_08260"
     CDS             complement(1060..>1812)
                     /locus_tag="PLJ82_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOR41534.1"
                     /translation="SHFLGQNFAKAFDVQFITKENKQEYVWATSWGVSTRLMGALIMT
                     HSDDNGLVLPPKLAPIQVVIVPIYKGEEQLQAINEKVDGIVKKLKALGISVKYDNADN
                     KRPGFKFAEYELKGVPVRLVMGGRDLESNTIEVTRRDTFEKQTRSCDDIDTFVKQLLD
                     DMQQTILQKALDLRTSLTCTVETYDEFKEKIEEGGFILAHWDGTSETEEKIKEETKAT
                     IRCIPLEGDKTPGVCMVTGKPSAQRVLFARSY"
BASE COUNT          625 a          351 c          283 g          553 t
ORIGIN      
        1 accaatgata acactcgaat atctttatta atacttggga acacatctcc aagatacatt
       61 tcggtcataa taggagaaaa agcaaaaaca aaaatggcga gagaaaaaag aaaaaggaga
      121 aagaagcgac caattttgtg aaattgagca aatgcaagtt ctttattttc cttaaaagtg
      181 ttgccaaaat gagggaaaag agcttgtcca atgggagtga tcatcatttg aaacagtcgc
      241 aaaaatttat ctgccaaggc ataatagcca actaaagtat aattgcccgt cataaaaccc
      301 aatataagca cattcgtttc tctgtataaa gttataccta atgtagataa aaacagagtc
      361 caactgcttt tcaattgcag tagaatttcg ctatatgtag ggatctttac aataagctga
      421 tagcgtttca tagcaacaaa aaatgccaaa gctgcaccaa acaaataacc aacagattga
      481 aaaagagtaa tgtaataata atctgctata cctttgacaa aaatataaat aaaaacaata
      541 ggaataagac gagtgacgag ggtaataata gttataaact tcatctcctc catgccttga
      601 aaaagccaag tgggatgaag agattgccct acaaaaacgc ctataccgta taaaataaga
      661 attgaatggg cattaaactc aggaattagc cacacacaca ctaccacgat aatactactg
      721 actacagcaa gcaataccct actagcagta atactataaa agatagcaga aagataaagt
      781 ttatcgtttt tatggatggc tacttgtttg gtagcataca aatcgtatcc ataattgacc
      841 aacataatga aataactaat aaaagtccat gcaaaaatat aaacaccgta atattcagct
      901 cctaaaaccc tgattaaata gggtattaac agcaaaggag aaagaagatt aactgcattc
      961 atcagcgtga gagctaatat attttctcca actttcttct ttttcttgtt ctgaataaat
     1021 gttgttaata tttcttttat cgccattatg atattttgat taatacgaac gtgcgaacaa
     1081 taccctttgt gcagaaggtt ttcctgtaac catacacact ccaggagttt tatctccctc
     1141 taacggaata caacgaattg ttgctttggt ttcttcttta attttttctt cagtttcgga
     1201 agtaccatcc caatgggcaa gtataaaacc tccttcttca attttttctt taaactcgtc
     1261 gtacgtttcc acagtgcaag ttagagatgt tctcaaatca agtgcttttt gcaaaatagt
     1321 ttgctgcata tcatccaaca attgtttcac aaaagtatct atatcatcac aactacgtgt
     1381 ttgtttttca aacgtatctc tacgagtaac ttctatggta ttgctttcta aatcgcgacc
     1441 acccattacc aatcgtacag gcactccttt gagttcgtat tcggcaaact taaatcctgg
     1501 tcgtttgttg tcagcattgt cgtattttac acttatgccc agtgctttca atttttttac
     1561 tattccatcc actttttcgt taatggcttg taattgttct tctcctttat aaataggtac
     1621 aattaccacc tgaatagggg ctaattttgg aggcaatacc aatccattat catcgctgtg
     1681 tgtcattatc aatgctccca tcaaacgggt agaaacaccc cacgaagtag cccacacata
     1741 ttcttgtttg ttttctttgg taataaactg cacatcaaaa gctttcgcaa aattttgtcc
     1801 taaaaagtgc ga
//
LOCUS       DAOSRP010000176         1764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_62291_length_1764_cov_3.289058, whole genome shotgun sequence.
ACCESSION   DAOSRP010000176 DAOSRP010000000
VERSION     DAOSRP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62291_length_1764_cov_3.289058"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..>1764
                     /locus_tag="PLJ82_08265"
     CDS             105..>1764
                     /locus_tag="PLJ82_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41535.1"
                     /translation="MNTSRTSTSFLYPLLSWNFVLRLLFIGLFLVVSLANTFAIKVRN
                     DNAWASVRIHYWNGGTGSDWGSRPTMTAATTPAWDTSGKSWVEWNINTCTSIKLTGAN
                     EPPSTNDINGVSNDGCYTMSSDATPTLTRVDCATGGAYVPPVPVINFAVTGGVVSGTT
                     EWNTSPPSSCIFEETGTNTYSLCFIYDSKPFKLLRKENGLPNTNWDWSSAVAIVAGSN
                     VSGSSGDLTITGFTTGDPVCLQLVVSGTTYTLSAEQSVYITSDLPNPINQGADITLTA
                     NNVSSACTWEYSTDGGTTWQPYTGATSGTYNEEISPSPLTNMTYRVTSDGKTATYMVQ
                     VVINCQGSIQTHFTETFGTLPTATARTTSAHIHSSVYQYSPEGKEVHDGWYAVVANPR
                     NCGRGTRQSTIVDGVVTVLGDVRTSGDYWYNDRTDHTGDTHGGMLFVNCKDKGEIIYQ
                     RTITDLCANIYMNFSAWFANAAVPSSTAPINTQFRILDQNGDEIQSARINVNNIKASD
                     GWVHGSTAFFSGNNTQLTLQIINNGEAGVGNDILIDDISFTSCVP"
BASE COUNT          534 a          351 c          357 g          522 t
ORIGIN      
        1 actattcaaa cgataaactt tttctttcaa gaaattttgt atttcattta ttttttttta
       61 actttggggc atatactgtt acgctaacct taactcaaca tagtatgaat acatcacgta
      121 ctagcacgtc ctttttgtac ccactcctct cgtggaattt tgtattacgc ctattattta
      181 ttggactatt tttagtagtc agtcttgcaa atacctttgc aataaaagtt agaaacgata
      241 acgcgtgggc ttccgtccgc atacattatt ggaacggagg aactggttct gattggggtt
      301 ctcgcccaac gatgacagct gccactacac ctgcttggga tacaagtggt aaaagctggg
      361 tggaatggaa tattaatacg tgtacgagta ttaaactaac aggggcaaac gaacctccca
      421 gtacaaatga catcaatggc gtaagtaatg atggttgcta caccatgagt tctgatgcta
      481 cccctacatt aacacgagtt gattgtgcta ctggtggagc gtatgtaccg cctgtacctg
      541 taataaattt tgcagtaact ggtggtgtag taagtggcac taccgaatgg aacacatctc
      601 ctccttcatc atgtattttc gaagaaacag gaaccaacac ctattctctt tgctttattt
      661 acgactccaa accatttaaa ctgttacgaa aagaaaacgg gctgccaaat acgaattggg
      721 attggtcaag tgcagttgcg attgtagcag gaagtaatgt gagtggtagt tctggagatt
      781 taactattac cgggtttact acgggagatc ctgtctgctt acaactagtc gtatctggaa
      841 ctacctacac attatcagca gagcaaagtg tatatattac gtctgacctt cctaatccaa
      901 taaaccaagg agcagatatt acattaacgg cgaataatgt ttcttctgct tgtacatggg
      961 aatattcaac cgatggtggt acaacatggc aaccttatac tggagcaacc agtggcacat
     1021 acaatgaaga aatatctcca agccctctta ccaatatgac atatagagtt acatctgatg
     1081 ggaagactgc tacgtatatg gtacaagttg ttattaactg ccaaggaagt attcaaactc
     1141 attttacaga aacttttggc actttgccta cagctactgc acgaacaact tctgctcata
     1201 tacattcatc agtttatcaa tatagccctg agggaaaaga agtacacgat ggttggtatg
     1261 ccgtagtggc taaccctcgt aattgcggta gaggaacaag acaatcaact attgtagatg
     1321 gcgtagttac tgtattagga gatgtacgta ccagtggtga ttattggtac aacgacagaa
     1381 ccgaccatac aggggacaca catggtggta tgctatttgt gaactgcaaa gacaaaggtg
     1441 agattattta tcaaagaacc attactgact tatgtgccaa tatttatatg aatttctctg
     1501 cttggtttgc caatgcagct gttccgtcgt ccacggcacc tatcaataca caattccgta
     1561 ttctcgacca gaatggagat gaaatacaat cggctcgtat caatgtaaat aatatcaaag
     1621 ccagtgatgg ttgggtacat gggtctacgg cttttttctc aggcaacaat acacaactta
     1681 ccttacagat aatcaacaat ggagaagctg gagtaggtaa tgacatttta attgatgata
     1741 tttcctttac atcttgtgtc ccaa
//
LOCUS       DAOSRP010000177         1648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_68849_length_1648_cov_4.839925, whole genome shotgun sequence.
ACCESSION   DAOSRP010000177 DAOSRP010000000
VERSION     DAOSRP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68849_length_1648_cov_4.839925"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1050)
                     /locus_tag="PLJ82_08270"
     CDS             complement(199..1050)
                     /locus_tag="PLJ82_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOR41536.1"
                     /translation="MAWVNFHSHTSYCDGIAQPEAYVLQAIELNMPAYGFSGHAPMPY
                     STNWAIKQDQLTSYINEIQRLKEVYTSQIDIYCAVEADYIPQITGPHLFINYPLDYII
                     GAIHFVGYFADGKPFSVAGKALDFDKGLKEIYGNDIKRLVKAYFSLNCELMLEDTPTI
                     LAHADLIKNGFQDSGIQHEKWYQQALFDMIDVAAERDVIIEINTRGLYKQRTSELYPC
                     EQALRYMKQKGVRMMLSADTHLLTELTSGFEYAVGVLQNNGINEITVFRKGQFVEIPF
                     SSTGFDY"
     gene            complement(1130..1492)
                     /locus_tag="PLJ82_08275"
     CDS             complement(1130..1492)
                     /locus_tag="PLJ82_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOR41537.1"
                     /translation="MKIAVPVKSTNQIDAHFGHCEGYKVFTIENNNVIAEENVPSPKG
                     CGCKSDIASVLHQKGVTVMLAGGIGDGAVQVLSSNGIQVVRNCKGDANAVLVEYLQGN
                     IVDGGVNCSHDHNHQCSH"
BASE COUNT          520 a          325 c          277 g          526 t
ORIGIN      
        1 gaggtctaca tcacgtcaaa ttgtaacagc tttgtaaaat ctctataaaa atctgttaat
       61 ttatagacaa agagtaagct cgtattgcca gaaactacaa aatcatagta tttttgctta
      121 tgaatttttc atagtatttg atttttaagg ttagcaagta ggcagtcagg cgagactgcc
      181 ttttgtgttt ttataccatc aataatcaaa cccggtagaa gaaaaaggaa tttccacaaa
      241 ctgcccttta cgaaatacag taatctcgtt gatcccatta ttttgtagta ctcctacggc
      301 atattcaaaa cccgaagtta gctctgtaag taaatgagta tcggcactga gcatcattct
      361 cacccctttt tgtttcatat accgcaatgc ctgttcgcag gggtatagtt ctgaagtacg
      421 ttgtttatat aatcctcgtg tattaatttc gataattaca tctcgttcag ccgccacatc
      481 aatcatgtcg aacaaagcct gttgatacca tttttcgtgt tgaataccac tgtcttgaaa
      541 cccattctta atcaagtcgg catgtgctaa aattgttggt gtatcctcga gcattagttc
      601 acaattgagc gaaaaatagg cttttaccaa gcgttttata tcgttgccat aaatttcttt
      661 tagtccttta tcaaaatcta aagctttgcc agctacgcta aaaggttttc catcagcaaa
      721 ataaccaaca aaatggatag ctccaatgat ataatctaag ggataattta taaacagatg
      781 cggacctgtt atttgtggaa tataatcagc ctctactgca caataaatat ctatttggga
      841 cgtatatact tctttaagtc gctgtatttc attaatatac gatgttaatt ggtcttgttt
      901 tatcgcccaa tttgtactat atggcatagg agcatgacca gaaaatccat aggcaggcat
      961 gtttagctca atagcttgca acacatacgc ctctggctgt gctatcccat cgcaataaga
     1021 agtgtgtgaa tgaaaattga cccaagccat actaaaacta ttaactacaa agatacggta
     1081 attttccctt tacaaaaagt gaacacatat caaaataaaa aatatattct taatgagagc
     1141 attgatggtt gtgatcgtgg ctacaattca ctccaccatc cacaatattg ccttgtaaat
     1201 actctaccaa aacagcattg gcatctccct tgcaattacg caccacttgt ataccattgc
     1261 ttgataacac ctgtacagca ccatcgccaa taccaccagc tagcattact gtaacgcctt
     1321 tttggtgaag aacactggca atgtccgatt tgcatccaca gcctttgggg gaaggaacat
     1381 tttcttcagc aataacattg ttattctcaa tagtaaatac tttgtagcct tcgcaatgac
     1441 caaaatgagc atcaatttga ttagttgatt ttacaggaac ggcaattttc atacgattat
     1501 agttttaaaa ttagtaattt agttgttttt gccaaccaca ataaaaggct tccaactgac
     1561 tcgaaagtgt ttgttggagt aattcaatag aattggctcc cgtacaatgt ccagtaaaaa
     1621 actgggtgga gggatatgtt tgttttaa
//
LOCUS       DAOSRP010000178         1600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_71954_length_1600_cov_4.387702, whole genome shotgun sequence.
ACCESSION   DAOSRP010000178 DAOSRP010000000
VERSION     DAOSRP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71954_length_1600_cov_4.387702"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLJ82_08280"
     CDS             complement(<1..755)
                     /locus_tag="PLJ82_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR41538.1"
                     /translation="MKLALKNPIVFFDLETTGTNIVSDRIVEIAYLKIYPNGNEESKR
                     YLVNPEMPIPQEVTAIHGISDEDVKNEPVFKELAKIIAKDIEGCDLAGYNSARFDLPL
                     LAEEFLRANVDIDLAKRKMIDVQVIFHKMEQRTLSAAYKFYCNSELTNAHTAEADTRA
                     TYEVLKAQLDRYDNLKNDVAFLADFSAQHKNADLAGRIIFNDKNEEVFNFGKYKGRRV
                     EDVLNEDMGYYGWIMKGDFSLYTKKVLTEVKLRG"
     gene            complement(782..>1600)
                     /locus_tag="PLJ82_08285"
     CDS             complement(782..>1600)
                     /locus_tag="PLJ82_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOR41539.1"
                     /translation="KNNKNSFDDIFTYKFDFHKTWMFRLLNPTNEFLFEIANFYFAKE
                     MYENALSALLELEEHTEPSADLYRKIGFSYQKTYNYSLAIEYYLKAELIETENIWTIR
                     KIAYCYRNLKQTDKALEYYLLADSLQPNTIRLLFTIGVCYVEMQDYDTALRYFYKIEF
                     LESNHKKSENYIALCSFLQKKYSQATKYYHKQPDKNTFYELLYIGHAYWCLGNKKEAL
                     HYYLQSAYTVKKETKQFKNFYEALEQDTDILLKHGFSLKDIVFIGDAVRMKIES"
BASE COUNT          490 a          291 c          250 g          569 t
ORIGIN      
        1 cctcttagtt ttacttcggt aagcactttt ttggtataga gcgaaaaatc gcctttcatt
       61 atccagccat agtagcccat atcttcgttc aacacatctt ctaccctacg ccctttgtat
      121 ttaccaaaat taaaaacttc ctcatttttg tcgttgaaaa taattctccc tgccaaatca
      181 gcatttttgt gttgggccga aaagtcagct aaaaaagcca catcgttttt caaattatca
      241 tacctatcta attgagcttt taatacttcg taggtagcac gtgtatcagc ttcggctgtg
      301 tgtgcattgg ttaattcgct attgcaataa aatttgtatg ctgccgacaa ggtacgttgt
      361 tccatcttgt gaaaaatcac ttgcacatct atcattttgc gtttcgccaa atcaatatct
      421 acgtttgctc tcaaaaattc ttcggccaat aagggcaaat caaaacgcgc cgaattgtat
      481 ccagccaaat cgcacccttc aatatctttg gcaattattt tagcaagttc tttaaaaacg
      541 ggttcatttt ttacatcctc atcactgata ccatgaatag cggtaacctc ttgggggata
      601 ggcatttctg gattaaccaa atatcgtttt gattcttcgt tgccatttgg ataaattttc
      661 aaatacgcga tttcaacgat tctatcagat acaatattgg tgcccgtggt ttctaaatca
      721 aaaaatacaa tggggttttt taatgctaat ttcatgtgta taaggttaaa tgatttgtgt
      781 gttatgattc aattttcatg cgtacagcgt cgcctataaa aacaatgtcc ttgagcgaaa
      841 aaccatgttt caacaaaata tcggtatctt gctccaacgc ttcataaaag tttttaaatt
      901 gtttagtctc tttttttact gtataagcac tttgtagata atagtgtaaa gcctcttttt
      961 tatttcctaa acaccaataa gcatgaccaa tgtaaagtaa ttcataaaag gtatttttgt
     1021 ctggctgttt atggtaatat ttggtagcct gagaatattt tttttgtaaa aatgaacaca
     1081 aagcaatata attttcgctc tttttatggt tcgattctaa aaactcaatt ttataaaaat
     1141 aacgcaaagc agtgtcataa tcttgcattt ctacataaca aacaccaatg gtaaacaaca
     1201 accgaatagt attgggttgc aaactatcag ccagcaaata atactctaag gctttgtccg
     1261 tttgttttaa attgcgataa caatatgcta ttttgcggat tgtccatata ttttcagttt
     1321 caatcagttc tgcttttagg taatactcaa tggctaacga ataattgtag gttttttgat
     1381 agctaaatcc aattttacga tacaagtcag cagagggctc tgtatgttcc tctaattcca
     1441 ataaggcaga aagggcattt tcatacattt cttttgcaaa atagaagttt gcaatttcaa
     1501 acaaaaattc gtttgttggg tttagtaaac gaaacatcca tgttttgtga aaatcaaact
     1561 tataggtaaa aatatcgtca aaactgtttt tgttattttt
//
LOCUS       DAOSRP010000179         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_73175_length_1582_cov_4.932547, whole genome shotgun sequence.
ACCESSION   DAOSRP010000179 DAOSRP010000000
VERSION     DAOSRP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73175_length_1582_cov_4.932547"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLJ82_08290"
     CDS             <1..189
                     /locus_tag="PLJ82_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41540.1"
                     /translation="LFWLTVGGCHYGIRFNQCANVNHLFSTIFPASFIIIIPFSICPN
                     THALFWQQMGMEYNPDCT"
     gene            complement(283..942)
                     /locus_tag="PLJ82_08295"
     CDS             complement(283..942)
                     /locus_tag="PLJ82_08295"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOR41541.1"
                     /translation="MKQPTRRFAYVAIALISFVTNTYSMGIIEAFYKFPVEIAFQLPS
                     SQKTAILTSYFSNQSAMATSRYNTPVQLMMVDTLGNYMQYQTSSASTTELKTITATNG
                     KTYIFHNAITCGPVCDSHITIFDENWYYVPIQQIAPALIKLQVSDFFDVTTATSEGID
                     IADLLTSIRPVFYELNFAAQHNDLLVTLHLNALPENTRQQLQPYYHQTIRLSWNGERF
                     E"
     gene            complement(964..>1582)
                     /locus_tag="PLJ82_08300"
     CDS             complement(964..>1582)
                     /locus_tag="PLJ82_08300"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOR41542.1"
                     /translation="TGAGRTDTGVHANMMVAHFDTHTAIDDCTRLVGKLNAFLPQSIA
                     IFAIKQVQPTAHARFDALSRTYQYHITTQKNPFIHQVSCKVPPTIDVNKMNTAAEKLL
                     QYADFTSFSKLHTDVKTNICTIHQAQWTQVSDTEWVFTIQADRFLRNMVRAIVGTLFD
                     VGNGKITIEEFCAIIEQKNRCAAGTSVPAHALFLVDVAYPTTIFV"
BASE COUNT          395 a          277 c          387 g          523 t
ORIGIN      
        1 ctattttggt tgacggttgg gggttgccat tatgggattc gtttcaatca atgtgctaat
       61 gttaaccatt tgttttcaac cattttcccg gcatcgttca ttatcattat cccattttca
      121 atatgcccaa atacgcatgc cctgttttgg caacaaatgg gtatggaata caatcctgat
      181 tgtacataat tatatccttt caggcgtata ctggggcggt gatgtttggc agggttatag
      241 gtctttaatt gctttttctt ttgcgttgag agattcttct ccttattcaa atcgctcgcc
      301 attccatgat aggcgtatgg tttggtggta atagggctgt agttgctggc gtgtgttttc
      361 tggcaaggcg ttaaggtgca gggttacgag caaatcgttg tgctgagctg caaaattgag
      421 ttcgtaaaat acgggacgta tgctggtaag taagtcagca atatctatac cctctgatgt
      481 ggctgtagta acgtcaaaaa aatcgcttac ttgcaatttt atcaaggctg gtgcaatttg
      541 ctgaatgggc acatagtacc aattctcgtc aaaaatggta atgtgcgagt cgcatacggg
      601 tccgcaggta atggcattat gaaaaatgta ggttttgcca ttggtggcgg taatggtttt
      661 gagttcggta gtgcttgctg atgaagtttg gtactgcatg tagttgccaa gggtgtctac
      721 catcatcagt tgcacaggtg tgttgtacct acttgtagcc atagcagatt ggttagaaaa
      781 atagctggta aggatagctg ttttttgtga actgggtagt tgaaacgcta tttctactgg
      841 aaatttgtaa aaggcttcga taatgcccat gctataagtg tttgttacaa agcttatcaa
      901 tgctattgct acgtatgcaa atcgacgagt aggttgtttc atgtaggtta tttttgcaca
      961 aatttacaca aaaattgtgg tagggtatgc tacatcaact aaaaaaaggg catgagctgg
     1021 cacagaagtc cccgctgcac agcggttttt ctgttcgata atagcacaaa actcctcgat
     1081 ggttattttt ccgtttccca catcaaacag agtgcccaca atggctcgca ccatgttgcg
     1141 taaaaatcgg tcggcttgta tggtaaatac ccattcagta tcgcttactt gtgtccattg
     1201 ggcttggtgt atggtgcaaa tattggtttt tacgtcagta tgtaatttgc taaaactcgt
     1261 aaaatcagca tattgcagta gtttttctgc tgcagtgttc attttattta catcaatggt
     1321 tggtggtact ttgcaactta cttggtggat aaaaggattt ttttgtgtag taatgtggta
     1381 ttgataggta cgtgagagtg catcgaaccg agcgtgagca gtgggttgta cttgtttgat
     1441 agcaaaaatg gcaatggatt gtggtaaaaa tgcgtttaac ttgcctacca gccgtgtgca
     1501 atcgtctata gccgtgtgcg tgtcgaaatg ggcaaccatc atgttggcgt gtacacccgt
     1561 atctgtgcgt ccagctccag ta
//
LOCUS       DAOSRP010000180         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_75167_length_1553_cov_3.848465, whole genome shotgun sequence.
ACCESSION   DAOSRP010000180 DAOSRP010000000
VERSION     DAOSRP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75167_length_1553_cov_3.848465"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..834)
                     /locus_tag="PLJ82_08305"
     CDS             complement(145..834)
                     /locus_tag="PLJ82_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOR41543.1"
                     /translation="MIISAFKILFFTDKKIGNENVEHVEKMFQSSYATKITCVSSIQQ
                     AMEELESTCIHILISDTTENTPKFASLIKKCKSSISCSHLYIMYITSSTEEDFILAMY
                     KHGVSEVVNLHTPEAILQARANKLAEHIIRCTNAHHKEELSLDPLLLSVSKGDITLQF
                     TKKEFDILHLLSNHPNKTFSRSEIYQKVWGNGVIVSDRTLDVHISKIRTKIGEQFIQT
                     YKGVGYRFMPR"
     gene            complement(831..>1553)
                     /locus_tag="PLJ82_08310"
     CDS             complement(831..>1553)
                     /locus_tag="PLJ82_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HOR41544.1"
                     /translation="AETLGMRVLLNDPPRQEKEDLPHFVSIHEIAEKANFISYHTPYT
                     NSGVYPTHHLFDQVYCKQLKKKPIIINAARGEIVDTTTLLNALHTRTITDCVLDCWEN
                     EPHISAPLAQQAGIATPHIAGYSADGKATATRMMITALTDYFGINIQKINPFSIPLPI
                     KSSIFANSNQMQDALYELVSQTHPILQESCQLKNDVSAFEYLRNHYPIRREFFAYTVH
                     VKKQQLHVVPILQQLGFNIQYT"
BASE COUNT          443 a          249 c          329 g          532 t
ORIGIN      
        1 gttgttttag tagggtagaa aaagattttt cgagcacttc ttgcacactt attccattgg
       61 gttgtatttg ccaaccgtga tacgagctac cattgtaggc tagatacaca aaatagcgaa
      121 aataaggctg ttcgttcata gagctcaacg tggcataaaa cggtatccta cacccttgta
      181 tgtttgaata aactgttccc caatttttgt gcgaatctta ctaatgtgta catctaaggt
      241 tctgtcgctt acaataacgc cattacccca tactttttgg taaatctcgg agcgagaaaa
      301 ggttttgttg ggatgattgg ataataagtg taagatatca aattcttttt ttgtaaattg
      361 cagtgtaata tcacctttgc ttacggataa tagtaaaggg tcgagcgata attcttcttt
      421 gtggtgggca ttggtacacc gtataatatg ttcggcaagc ttgttcgctc ttgcttgcag
      481 aatagcttct ggggtatgca gatttaccac ttcgcttaca ccgtgtttgt acattgcaag
      541 gataaagtct tcctctgtag aggaggtaat atacataata tacagatgac tacaggatat
      601 cgaagatttg cattttttta ttaaactagc aaatttgggc gtattttccg tagtatctga
      661 gattaaaata tgaatgcagg tagattctaa ttcttccatc gcttgttgta tcgacgatac
      721 acaggtaatt tttgtggcat agctagattg aaacatcttt tcaacatgct ctacattttc
      781 gttaccaatc tttttgtcgg taaaaaataa aattttaaat gcagaaataa tcatgtgtat
      841 tgaatattaa aaccaagctg ttgtaaaatg ggcactacgt gtaattgttg tttttttaca
      901 tgaacggtgt aagcaaaaaa ttctctacga atagggtaat gattacgcaa gtattcaaaa
      961 gcagacacat cattctttaa ttggcaactt tcttgcaaga taggatgtgt ttgactaaca
     1021 agttcatata aagcatcttg catttgattg ctgttggcaa aaatactact ttttattgga
     1081 agtggtatcg aaaatgggtt aattttttgt atatttattc caaaataatc tgtaagtgca
     1141 gtaatcatca tacgagtagc agttgcctta ccatcagcag agtaacctgc tatatgtggg
     1201 gtggcaatgc ctgcttgttg tgctaaggga gcagaaatat ggggttcatt ttcccaacaa
     1261 tcaagtacac agtcggtaat ggtgcgtgtg tgtaatgcgt tgagtagggt agttgtatct
     1321 acaatttcgc cacgagctgc attaatgatg atgggttttt ttttgagttg tttgcaatat
     1381 acttggtcga aaagatgatg cgtagggtac acgccactgt tcgtataggg tgtatgatag
     1441 ctgataaagt tggctttttc ggctatttcg tgtatgctca caaaatgagg taaatcttct
     1501 ttttcttgtc gtggtgggtc gtttagcaac acacgcatac caagagtttc tgc
//
LOCUS       DAOSRP010000181         1419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_86078_length_1419_cov_3.191349, whole genome shotgun sequence.
ACCESSION   DAOSRP010000181 DAOSRP010000000
VERSION     DAOSRP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86078_length_1419_cov_3.191349"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLJ82_08315"
     CDS             complement(<1..1019)
                     /locus_tag="PLJ82_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BspA family leucine-rich repeat surface protein"
                     /protein_id="HOR41545.1"
                     /translation="MKKETLLFLKEQNNYKFEQIKPKVLFSHPLKEAKNRLLQKISAL
                     LLFLSLALSINAEITKGSPNNFITVWDTENTTIIVIPTRGGGYNYHVYWEQENNPAIN
                     GTLLNQTGNATISDLQPNTRYRVEIAGIFPQFYMNYNNTQPTQLRTITQWGIIPWRSM
                     EYAFLGCSNLDITATDVPNLSNLTSLEYMFVDCSSLTGIDANWNWNTANVTNMRGMFY
                     NASNFNQPIGNWNTSNVTDMSNMFSYSSIFNQPIGNWNTAKVTNMSNMFYNASSFNQP
                     IGDWNTSNVTDMNRMFVRASSFNQPIGNWNTSNVTKMYSMFEYAISFNQSIYSWNTSN
                     VTNMR"
BASE COUNT          390 a          246 c          274 g          509 t
ORIGIN      
        1 catctcatat ttgttacatt ggaagtattc caactatata ttgattgatt gaagctaata
       61 gcatattcaa acatcgaata cattttcgtt acgttggaag tgttccagtt gccgatgggt
      121 tggttgaaac tgctagctct aacgaacata cgattcatat ctgtaacatt ggaagtgttc
      181 caatcgccaa tgggttgatt gaaactactg gcattataga acatattgct catatttgtt
      241 acctttgcgg tattccaatt accaatgggt tgattgaaaa tgcttgaata agagaacata
      301 ttgctcatat ctgttacgtt agaagtattc cagttaccaa taggttgatt gaaattactg
      361 gcattataaa acatacctct catatttgta acatttgcag tattccaatt ccaattggca
      421 tctataccag ttaggctaga gcaatcaaca aacatatatt caagactcgt aagattgctt
      481 aaattaggta catctgtagc agtaatatct aaattactgc accccaaaaa agcatattcc
      541 atacttcgcc atgggataat accccattgg gtaatagtgc gtagttgagt aggttgagta
      601 ttattataat tcatataaaa ctgagggaaa atacctgcaa tttctacccg atagcgtgta
      661 ttgggttgta agtccgatat cgtggcattg cctgtttggt ttagtaacgt gccattgata
      721 gctgggttat tttcttgctc ccaatatacg tggtagttgt aaccaccacc tctggttgga
      781 attacgataa ttgtagtatt ttcggtatcc cacacggtga tgaaattgtt gggggagcct
      841 tttgtaattt cagcattgat agataatgcc aatgataaaa agaggagaag ggctgagatt
      901 ttttgtaata acctgttttt tgcttctttt aatgggtgcg agaatagtac cttgggcttg
      961 atttgttcaa acttgtaatt gttttgttct tttaaaaaga gaagggtttc ttttttcatt
     1021 ggaatatatt ttttaatggt ctgtatgttt ttatttttat cggggttaat gcccgttatt
     1081 ttgtacacat ttattgttaa ttaagatgtc agggctacgc ccctacattt gttaaattta
     1141 tttgtttgtt acgaagattt cagggctacg cccctagatc gttgtttcgt gatattttgc
     1201 tatgaagaca acagggctaa cgcccctttc tttactctca acttttcatc gcaacgaaga
     1261 tttcggggct acgctcctag attcgttatt tcgtcatatt ttgctacgaa gatatcaggg
     1321 ctacgcccct acatcgttgt ttgttcattt tttctacgaa gatatcaggg ctaacgcccc
     1381 tacattcgtt ttttcgtgat attttgctac caagacaac
//
LOCUS       DAOSRP010000182         1383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_89411_length_1383_cov_11.159639, whole genome shotgun
            sequence.
ACCESSION   DAOSRP010000182 DAOSRP010000000
VERSION     DAOSRP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89411_length_1383_cov_11.159639"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1383)
                     /locus_tag="PLJ82_08320"
     CDS             complement(<1..>1383)
                     /locus_tag="PLJ82_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41546.1"
                     /translation="GNNILYEYYNAWNDTANTWIGNYKEVSAYGSDNNPLSVESYNWS
                     DSTGTWMENEKFTYTYARGIQLDYYHYVWDTDSNDWRAIEKTIGTIHPNGGISLRINY
                     QWNVSVWDTISKWETMAYDTLGHWLETEVIYYEYNSTWEEQEKTTYTWDSNDKCTEKI
                     IYRYISNNWEKYRKYERNHDIYGNETMYACYNWSSGAWVGSGNKFEYAYDANGIQTMY
                     ARYSWSSGKWEGNYKKENAYDANGYETLAAYYNWNDSVWVENYKYEWAYDANGYQTLN
                     AYYTWGDSVWVGNYKYERAYDANGNQTMIAYYNNWSSGKWIGNYRNEWAKDANGNSLL
                     DVYQNGWDSTWKYGYKYVYAYDINENQTLSEYSVWDTISRIWVKANKQEWAYKDTYIY
                     FTYDFPLYDRYYEWDSTAWELQKSTEWIYNTVGDIIEVIYSELFNDSLKVKTRDVTTT
                     DTTHLRASLVF"
BASE COUNT          376 a          306 c          193 g          508 t
ORIGIN      
        1 aaatactaaa cttgcacgta aatgagtggt atcggtagtg gttacatctc tcgtttttac
       61 tttcaaagaa tcattgaata gttcgctata aatcacctct ataatgtctc ctacagtatt
      121 ataaatccat tctgtagatt tttgcaattc ccatgccgta ctatcccatt cgtaatacct
      181 atcgtataaa ggaaaatcat acgtaaagta aatatacgta tctttataag cccattcctg
      241 tttatttgct tttacccata ttctgcttat agtatcccat acggaatatt cacttagtgt
      301 ttggttttca ttgatatcat aggcatatac atacttatac ccatatttcc aagtgctatc
      361 ccaaccattt tgatatacat ctaatagtga gttaccattg gcatccttag cccattcgtt
      421 tctataattt cctatccatt taccactact ccaattgttg tagtaggcaa tcatggtttg
      481 gttgccattg gcatcgtaag ccctttcgta tttataattt cctacccaaa cactgtctcc
      541 ccaggtgtag taggcattca aggtttgata accattggca tcgtaagccc attcgtattt
      601 ataattttct acccaaacac tgtcgttcca gttgtagtag gcagccaagg tttcatagcc
      661 attggcatca taagcgtttt cttttttata atttccttcc catttgccac tgctccaact
      721 gtatctggca tacatggttt gaataccatt ggcatcatag gcgtattcga acttattacc
      781 acttcctacc caagcaccgc tactccagtt ataacaggca tacatagttt cgttgccata
      841 aatatcatgg tttcgttcat atttcctata cttttcccaa ttattactaa tgtatcgata
      901 aataattttt tcagtacatt tatcgttact atcccaagta taagttgttt tttcttgttc
      961 ttcccatgtc gagttatatt cataatatat tacttcggtt tccaaccagt gtcctaaagt
     1021 atcgtaagcc atagtttccc atttactaat tgtatcccat acacttacat tccactgata
     1081 attaatacgc aaactaatgc ctccgttagg atgaatagtg cctattgtct tttctattgc
     1141 tcgccaatcg ttactatccg tatcccacac atagtgataa taatccaatt gtattccacg
     1201 agcataggta taggtgaatt tttcattttc catccatgta ccggtgctgt cactccagtt
     1261 ataggattct actgataatg gattgttatc gctaccgtaa gcagatacct ctttataatt
     1321 tcctatccaa gtgtttgcag tatcgttcca agcattatag tattcataca atatgttatt
     1381 gcc
//
LOCUS       DAOSRP010000183         1381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_89603_length_1381_cov_23.212670, whole genome shotgun
            sequence.
ACCESSION   DAOSRP010000183 DAOSRP010000000
VERSION     DAOSRP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89603_length_1381_cov_23.212670"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLJ82_08325"
     CDS             complement(<1..1216)
                     /locus_tag="PLJ82_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR41547.1"
                     /translation="MKNSQFFMIATLSLMISVSAFSQNNGRERIKSPTSSMEVKQAMQ
                     ERQNKAKEQKAIRLQEREQQKEQRATRLREREAQRKEAEAKRSEMQEKMQPKQRQMHQ
                     QERNAMPKVAVRKKTTDKLQQKEVSKLALDSVVYPELWKETYRYDYYGRITEHINYNW
                     NVSKWEPSQKYEYVYEGDIIKEIYYYWDGSDWEPNSKYEYALDANGNEIMEAYYTWSS
                     GNWVGNYKYEYAYDANGDQIMEAYYNWSSGKWVGNYKYSIEYNNDIEIITYYIWDVVK
                     SQWQNDEKYEYAEDDYGNTLHDAGYSWNSTTNKWEGVYKQLYAYNSDNKKVLDEYYDD
                     WNDTTNTWIGYEKKVWAYNDNGKYILYEHYNAWNDTTNTWIGYEKEVWAYDDNGNNIL
                     YEYYNAWNDTANT"
BASE COUNT          337 a          283 c          201 g          560 t
ORIGIN      
        1 aagtgtttgc agtatcgttc caagcattat agtattcata caatatgtta ttgccgttat
       61 catcgtaagc ccacacctct ttttcatatc ctatccaagt gtttgtagta tcgttccaag
      121 cattatagtg ttcatacaat atgtatttgc cattatcatt gtaagcccat actttttttt
      181 catatcctat ccaagtgttc gtagtatcat tccagtcatc ataatattca tctaatactt
      241 tcttgttatc gctattgtaa gcatatagct gtttataaac cccttcccat ttattagtgg
      301 tgctgttcca actataacca gcatcatgca acgtatttcc ataatcatct tcggcatatt
      361 cgtatttttc atcgttttgc cattgtgatt taacaacgtc ccaaatgtaa taagtgatta
      421 tttcaatatc attattgtat tctatggagt atttataatt tcccacccac ttgccgctgc
      481 tccaattata gtaggcttcc atgatttggt cgccattggc atcgtaggca tattcgtatt
      541 tataatttcc cacccaattg ccgctgctcc aggtatagta agcttccata atttcgttac
      601 cgttagcatc aagagcgtat tcgtatttgc tgttaggctc ccaatcgcta ccatcccaat
      661 agtaataaat ttcttttatt atgtctccct cataaacgta ttcgtatttt tggcttggtt
      721 cccacttgct aacattccaa ttgtaattaa tgtgttcggt tatcctacca tagtagtcgt
      781 agcggtatgt ttctttccac aattccggat acactacgct gtctaacgcg agtttgctaa
      841 cttctttttg ttgaagtttg tcggttgttt ttttccttac ggcaactttt ggcattgcat
      901 ttctttcctg ttgatgcatt tgtcgctgtt taggctgcat tttttcttgc atttcggaac
      961 gctttgcttc ggcttctttt cgctgtgctt ctcgttcacg taagcgtgtt gctctttgtt
     1021 ctttttgctg ctctcgttct tgcaagcgta ttgctttttg ctctttggct ttattttgcc
     1081 tttcctgcat tgcttgtttc acttccatag aggaagtagg acttttgatt cgttctctac
     1141 cattattttg agaaaacgca ctgacactta tcattagact aagcgtcgct atcataaaaa
     1201 attgactgtt tttcatattc attattattt taaattttta agcaaaaata caaaaatatt
     1261 ttatacaata tggcactttt ttttatttag ctataaaatt tatgataaac taaaagtact
     1321 ataaataaaa tatttacatt ttttagctta aaaacttttc actgattttg cactcacaca
     1381 t
//
LOCUS       DAOSRP010000184         1050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_2264
            NODE_135311_length_1050_cov_5.370854, whole genome shotgun
            sequence.
ACCESSION   DAOSRP010000184 DAOSRP010000000
VERSION     DAOSRP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864331
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:01:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,666
            CDSs (total)                      :: 1,637
            Genes (coding)                    :: 1,619
            CDSs (with protein)               :: 1,619
            Genes (RNA)                       :: 29
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 3 of 18
            Pseudo Genes (incomplete)         :: 14 of 18
            Pseudo Genes (internal stop)      :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135311_length_1050_cov_5.370854"
                     /isolate="AMR_MDS_2264"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLJ82_08330"
     CDS             <1..889
                     /locus_tag="PLJ82_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOR41548.1"
                     /translation="ESKNSRMITMLATPFMSCSARLPVYVLFAGAFFPNSASLVMLGL
                     YLIGMLMAIISARLFKRFLFNTEDVPFVMELPPYRMPTARATLRHMWEKSQQYLRKMG
                     TIILFASIIIWALGYFPLAETRTPQLDDKINQLTALPTTEETNTQLQKLQWERNTQQQ
                     KTSYIGKIGNTILPIIKPLGFNWEMGIGLIAGLPAKELVVSTLGVLYTNNATDENSAE
                     LSYKLQHVTDASGKQQITPLIAFGYMIFILLSFPCIATIIAIKNESGSWKWGLFSIVY
                     STSLAWVCAFLIFQLGSLL"
BASE COUNT          330 a          223 c          194 g          303 t
ORIGIN      
        1 cgaaagcaaa aatagccgca tgataaccat gctagctaca ccctttatgt cgtgtagtgc
       61 tcggctaccg gtgtatgtgt tgtttgcggg ggcatttttc cctaacagtg cctcactcgt
      121 aatgcttgga ttatacctca ttggcatgct catggcaatt atatcggctc gtttgttcaa
      181 acgcttttta ttcaacaccg aagatgtgcc ttttgtaatg gaattgccac cataccgaat
      241 gcctacggct agggctaccc ttcgccatat gtgggaaaaa tcgcaacaat acttgcgtaa
      301 aatgggtacc atcatcttgt ttgcctctat cattatctgg gctttaggct attttccctt
      361 agcagaaacg cgtacaccgc aactagatga taaaatcaac caactaacag cacttcccac
      421 caccgaggaa acgaacacgc aactgcaaaa actgcaatgg gaacgtaaca cgcaacaaca
      481 aaaaacgagt tatattggca aaattggcaa tacgattctc cctattatca aacccctggg
      541 ttttaattgg gaaatgggaa ttggtcttat tgctggacta cctgccaaag agctagttgt
      601 tagcacattg ggcgtattat ataccaacaa tgcaactgac gaaaattcag ccgaactatc
      661 gtataaacta caacatgtaa ccgatgcctc gggcaagcaa caaattacac cgcttatcgc
      721 ttttggctac atgattttta ttctgttgag ttttccgtgc atcgccacta tcattgccat
      781 taagaacgaa tcgggctcgt ggaaatgggg attattctct attgtgtaca gcacatcttt
      841 ggcatgggta tgtgcatttc tcatttttca attagggagt ttgttataga aaaaaaataa
      901 tcttaaaaaa agtagtaatt tctaaaactt tattaccttt gcatagtaaa ttaacttagt
      961 ataaactact tatgcgtttg caaatacaat aagcgtgtaa gacaaaaaaa aacaaaaaaa
     1021 ctgtatgaaa aaaatcacta cactttgcat
//
