LOCUS       DAOSSL010000001        62972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_499_length_62972_cov_16.534577, whole genome shotgun sequence.
ACCESSION   DAOSSL010000001 DAOSSL010000000
VERSION     DAOSSL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 62972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62972
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_499_length_62972_cov_16.534577"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..1225)
                     /gene="metK"
                     /locus_tag="PLH17_00005"
     CDS             complement(26..1225)
                     /gene="metK"
                     /locus_tag="PLH17_00005"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOR20202.1"
                     /translation="MNEKILFTSEAVSEGHPDKLCDRISDAILDFCLEHDENARVACE
                     CFATTEYVLIGGEITCKVTPDYEKIARKVIREVGYTSPELGIGADTCTIDVRVKNQSL
                     DIAGAITHGPSLLDIGAGDQGIMFGYATKESKGYMPLPISIAQKLVRKASALRKEGKF
                     VHARPDMKSQVTIDYSDPDHIRVETVLMSIQHDPDYDKEKFINYVHREIMVPVVASFG
                     LNSDFNFLINPSGRFTTGGPMGDTGLTGRKIIVDTYGGSAKHGGGAFSGKDATKVDRS
                     GAYMARYVAKNLVAAGVADRLEIQLGYAIGVSEPLSINLSTFHTAKYTDDFILKIIRQ
                     LFDFRPGAIIAHFALNKPTFKYQKLAAYGHFGRPDLKLPWEQLDRVDDIKNLIKKENP
                     ERIKRVY"
     gene            complement(1457..2038)
                     /gene="pduL"
                     /locus_tag="PLH17_00010"
     CDS             complement(1457..2038)
                     /gene="pduL"
                     /locus_tag="PLH17_00010"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOR20203.1"
                     /translation="MKVLIETSARHIHLSSEHLEILCGKGFALQEKKPLSQPGQYVST
                     TRLDVVGPKSTIKNVVVLGPVRPKTQVEVSMTDARTLGVIAPIRESGDVAKSAPITIV
                     GPVGSIQIEEGVIIAKRHLHITPADAALAGVKSGDIVMIEVNTPERGIIFKDVVVRVS
                     ERFSTAVHIDTDESNAANASADMNAPLYGEIIK"
     gene            2165..>2370
                     /locus_tag="PLH17_00015"
     CDS             2165..>2370
                     /locus_tag="PLH17_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR20204.1"
                     /translation="MEKENVSTALLEELIKNRQVAKLRELFETIPTIDLAEAANDIED
                     IRDIVFIFKVVKSDYTAEFFTDLSS"
     assembly_gap    2371..2470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2471..3184
                     /locus_tag="PLH17_00020"
     CDS             <2471..3184
                     /locus_tag="PLH17_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOR20205.1"
                     /translation="NEDQRLTGIITIDDIVDVMEQEATEDMSRMALVTPLEDSYKDTG
                     VLKMAIKTMPWLIVLMILGVFSSVVLSSFQDVLSSLAILSVFIPVLMDTGGNSGGQTI
                     ALMIRGIAVGEFGPKDYGHILWKEFRVALLVGIMVSIAAFIIFIIEMYLGIVFYAPEG
                     IVGTALLFERMKISGMVSITLFFTIVISKTLGVTIPMIVKALKKDPAIISAPFITTIV
                     DVSALLIYFFICTQIFQLV"
     gene            3237..3320
                     /locus_tag="PLH17_00025"
     tRNA            3237..3320
                     /locus_tag="PLH17_00025"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3270..3272,aa:Leu,seq:gag)
     gene            3412..4389
                     /locus_tag="PLH17_00030"
     CDS             3412..4389
                     /locus_tag="PLH17_00030"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOR20206.1"
                     /translation="MKTILLALKRNKLMTALAAFLLVAVILSAATNYLVANQEPTSVI
                     QSFQTLDSLPDGQGQKVKVILLNGQSNASGVGSVHYLEEKSQSADYASFEDGYDNVMI
                     NFFSENGNYSSHGEFVKAKVGQGCTPDYFGPELGLADSLSQVFADELVILLKYTWGGS
                     NLHTQWRAPSMPGPTGDLYTAFINFTSAHMKYLLGKNYDASIGAMCWMQGESDAYLPY
                     ANDYKDGLSGFVTDIRADLGVYSGDDGIHFIDAGIYDSPLVDQYETINQAKRHVAALS
                     SKNHYFDTIEAGLEYDKEPADNPDLAHFDALSTLELGRLFANEISSIYS"
     gene            4542..5039
                     /locus_tag="PLH17_00035"
     CDS             4542..5039
                     /locus_tag="PLH17_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR20207.1"
                     /translation="MEESLGQRIARLRRNKGLTQEDLGYKVGVSSQAVSKWETDSSVP
                     DVTLLVKIANIFDISVDELLGNDRIKETPQIVPPAERKPIDKMILKIIIDSDEGDKVR
                     VNLPISLIKIMIEGGAKMPEVNGKDVLKNINWDNIFALIEQGAIGKLVEIEGSDGVHV
                     DIVVE"
     gene            5039..5278
                     /locus_tag="PLH17_00040"
     CDS             5039..5278
                     /locus_tag="PLH17_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20208.1"
                     /translation="MKVLVKSQEDNIRVWIPNLLINPRILRFILRKANVAVDDNLKRV
                     LPKLCLTLKKFRRHHKRFVLVEVITLKKEHIKITL"
     gene            complement(5336..6934)
                     /gene="gpmI"
                     /locus_tag="PLH17_00045"
     CDS             complement(5336..6934)
                     /gene="gpmI"
                     /locus_tag="PLH17_00045"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOR20209.1"
                     /translation="MKKPVILCIMDGYGIRHAEAGNAIFAAKKPNLDKIFASYPYTEI
                     ETSGESVGLPDGQMGNSEVGHLNIGAGRIVYQSLTLINKAVREGTFFANEKYLKAMDH
                     VKKHNSALHILGLVSDGGVHSHIEHFIAMLQMAKKNGLKKVFVHAFMDGRDVDPQIGA
                     SYIQRLQDEMDRLRLGAIADISGRYWAMDRDKNLDRVDASYRVIVDHEGPSFGDYQQY
                     FKDQYAFLPSKGMDASDEFVMPHYNRNVDGRIKDDDAIIFMNFRPDRAIEISTVFTNP
                     HFYGNPPQKPDGTLAFKPYTPKHILKNLFYVCTMKYAESVKGEIAFHLPTLDNVLGPY
                     LADKGYSQLRIAETEKYAHVTFFFDGTVNYDGVEKPALANSRRVLINSPKVATYDLQP
                     EMAAYKVKDALIKELNKGDLDVVILNFANCDMVGHTAIFPAVVKAVETIDACVGEIYN
                     WTQDNGGVMLITADHGNAEEVFDDFGRPITSHTKNIVPFCITKPGLKLMPKGGKLANI
                     APTILDLLGLEKPSEMNEKSLIIE"
     gene            complement(6947..7477)
                     /locus_tag="PLH17_00050"
     CDS             complement(6947..7477)
                     /locus_tag="PLH17_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20210.1"
                     /translation="MQEWLQWTLIAVAGVALLYLLFFFIVLLFISNFNKRLKKRREAM
                     RLLLAQRKDITLALYRKIVDQKIKVSIAVTNRINELSNKEIGLFNHYEIFEYAPELEK
                     VTKTIFEIAEANKVIKKSEEFAQFAKELEELDEMKRQHIAIYNADINGYNYWIKLMSY
                     RRILKALGFHEKKRLE"
     gene            7647..8489
                     /locus_tag="PLH17_00055"
     CDS             7647..8489
                     /locus_tag="PLH17_00055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR20211.1"
                     /translation="MDGKPIMAIIYDFDKTLTPEDMQNYSFIPALGMTPQEFWGKTGE
                     FSAKTGVERILSYMYMMICMAKEKGIKMTSEWLRSLGKDIKYFEGVTTWFNRINAYGL
                     DNGVKVEHYLTSSGTKEIIDGCSIAKEFKMIYGCEFLFDDETGEPIWPKFAVNYTQKT
                     QHLFRISKGVIEATDDDRLNEKTPVRRIPYRNIVYLGDGMTDIPMMILVKQNGGRSIA
                     VYPRGKKEKVANLFDDGRVNYICVADYSSNSELEKVMKLIIDSIAVASMLEKKEKSII
                     TKKE"
     gene            8492..9184
                     /gene="ispD"
                     /locus_tag="PLH17_00060"
     CDS             8492..9184
                     /gene="ispD"
                     /locus_tag="PLH17_00060"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOR20212.1"
                     /translation="MNIAIILAAGSSHRFNAEMPKQYYEIDGHPLIWYTIHSFHRVEA
                     IDLIVVVAQKDHIDYLSKLVQDSNFTKVKHIILGGKTRQESSFNGVKFVKTLAKDDDI
                     VLIHDAARPLISERIITDCIEGAKKHNAVTTVLPSDDTTVISSDGEQIGFMPERKNIY
                     RVQTPQAFKVGMIYIAHHLLKNENDSTDDAGLIFRTFMPVHLVMGDKKAMKVTSLEDV
                     YYIKGLLHGEKI"
     gene            9184..9588
                     /locus_tag="PLH17_00065"
     CDS             9184..9588
                     /locus_tag="PLH17_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOR20213.1"
                     /translation="MPEAFKVGDIIEGTITELRKFGALMIFEHDATGLLHISEISDRF
                     VYDIARYVQVGRNFNVKILEIDPNNGFMKVSLRKVTIEDRIDFKNSSKKRTVIAAEEI
                     DFAPLQEQLEKWTDKQLENTTEEESDAQSKSR"
     gene            9566..10846
                     /locus_tag="PLH17_00070"
     CDS             9566..10846
                     /locus_tag="PLH17_00070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOR20214.1"
                     /translation="MLKVNLDNLISPVDFVSYKEKVATIDRAISEKTGAGNDFLGWVD
                     LPLNYDKDEFENIRKYAAEVRANYDVLVVCGIGGSYLGARAAIDALRGLKSTDSLEII
                     FMGQTFSAEYTHEILTYLNDKRFAVNVISKSGTTTETSIAFRLLRNLLEQKIGKAKAR
                     RAIFATTDKNKGALKSLSDIEGYRTFVLPSDVGGRFSVLTAVGLFPIACAGIDIQELM
                     NGAIEARKMYANPDLSNNGCYQYAVARDHLYQHGKKVELYVVYEPKLLQLGEWLKQLF
                     GESEGKNQRGLFPASVSFSTDLHSLGQFVQDGSPILFETILFADKPKNDVLIPSDESD
                     LDGLNYLAGKSLAYVNEKAFLGTLEAHVKTGEVNNILLSVSEITPWTLGHLFYFFERA
                     CAMSAYLLGVNPFDQPGVEIYKSNMFRLLGKKGY"
     gene            10975..11050
                     /locus_tag="PLH17_00075"
     tRNA            10975..11050
                     /locus_tag="PLH17_00075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11008..11010,aa:Val,seq:tac)
     gene            11072..11156
                     /locus_tag="PLH17_00080"
     tRNA            11072..11156
                     /locus_tag="PLH17_00080"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11106..11108,aa:Tyr,seq:gta)
     gene            11164..11239
                     /locus_tag="PLH17_00085"
     tRNA            11164..11239
                     /locus_tag="PLH17_00085"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11196..11198,aa:Gln,seq:ttg)
     gene            11249..11324
                     /locus_tag="PLH17_00090"
     tRNA            11249..11324
                     /locus_tag="PLH17_00090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11282..11284,aa:Lys,seq:ttt)
     gene            11338..11425
                     /locus_tag="PLH17_00095"
     tRNA            11338..11425
                     /locus_tag="PLH17_00095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11372..11374,aa:Leu,seq:taa)
     gene            11607..12104
                     /locus_tag="PLH17_00100"
     CDS             11607..12104
                     /locus_tag="PLH17_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20215.1"
                     /translation="MNLIKKYGPYISMGLIVMALGFLFLPYLRTGYNNTYYAIGYEAI
                     FNIKESSIPSTVSNKGGPSVMLILAFVLMVVSLGSLPFHHKDPVISFLAGIALSISGV
                     IFFLGQVVMFINLRGSAISGTFGLYLVATLVTLAAAISLFVGISNLRAVNQKNNDNRY
                     SYIRK"
     gene            complement(12151..12540)
                     /locus_tag="PLH17_00105"
     CDS             complement(12151..12540)
                     /locus_tag="PLH17_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20216.1"
                     /translation="MEKRSSSFRISITHLKVLETIANLNREQAFPSVEGIAKILNGIS
                     DGETRKFSATSTFATLLSYRGRKLTSLVTNLVRRGYLSYLFEENSDVKFLKITDLGMA
                     TLILPEYAKKRVYKHKNKSDRRTILFK"
     gene            12610..12882
                     /locus_tag="PLH17_00110"
     CDS             12610..12882
                     /locus_tag="PLH17_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOR20217.1"
                     /translation="MDKELRNQIEATLNKIRPFIQRDGGDVKFVGFEDGIIYIEMAGA
                     CADCAFIDNTISDGIEIILMEEVPGIIAVKQAFEMPESARLESAEK"
     gene            complement(12956..13732)
                     /locus_tag="PLH17_00115"
     CDS             complement(12956..13732)
                     /locus_tag="PLH17_00115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOR20218.1"
                     /translation="MWENELRVAIRAALQARDKIMEIYKKPFDVEIKEDNSPVTLADK
                     MADEIIRQVIAQAFPTHAFLTEESSDDKSRLQNDYVWIVDPVDGTKDFVDKNDEFTTN
                     IALAYKHEVVVGVVLVPATGEIYYASKGNGSFYQKKNRIKIIRVNSKTRGLTVLTSRF
                     HVNEKELATIERHKDKIKHRKTFGSAIKPCRIARGLAEITYRLSAGTKEWDTAASQIV
                     VEEAGGVFVKPSGARILYNRDDVYNREGYVVANRIENVLL"
     gene            complement(13719..14687)
                     /locus_tag="PLH17_00120"
     CDS             complement(13719..14687)
                     /locus_tag="PLH17_00120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR20219.1"
                     /translation="MNEAQAAVFSLIEKYDSIVIFGHSLPDGDCYGSQIALREVLQTA
                     YPHKKIYAIGSGLSALFGRLGEMDIVSDELIAKSLAILVDVSDFPLVEDQRVLLAPDY
                     CKIDHHHESNPFLYDKIMDHGVIATAELIARIAFQRNILLSKRAAEAIFLGIVTDSGR
                     FLYAPTRKETFDIISRLFDYGLEMKPIYDVIYQTQENQLKYKGFLLSHYQKTENKVVY
                     CYAEPKDYQAYGLEFSYASSQVNVLANIQGSPIFALFTQNEKGGLRVELRSSGVPIRD
                     IAVKYGGGGHANAAGVRMEKGGYQEAMAIVKDLDLLAKENNDYVGK"
     gene            complement(14739..15599)
                     /gene="fba"
                     /locus_tag="PLH17_00125"
     CDS             complement(14739..15599)
                     /gene="fba"
                     /locus_tag="PLH17_00125"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOR20220.1"
                     /translation="MALVSMKEMLLKALKEKYAVGQFNINNVEWTSAVLEEAQELKAP
                     VILGVSEGAAKYMGGFNTIVGMVKGYIKDRNITVPVALHLDHGSSYEMCIKAIDAGFT
                     SVMIDASHHPLEENIEMTKKVVDYAHARGVSVEAELGRVGGQEDDVVAETMYAHPFEC
                     VRMVKESGIDCLAPALGSVHGPYKGEPKLGFKEMAEIRDLLVTTPLVLHGGSGIPDFQ
                     IKMAIERGTSKINVNTECQQEWTKITREVIALDSKVYDPRKIIGPGMKGIRKVVRAKC
                     EVFGCIGKAE"
     gene            complement(15623..16024)
                     /gene="rpoE"
                     /locus_tag="PLH17_00130"
     CDS             complement(15623..16024)
                     /gene="rpoE"
                     /locus_tag="PLH17_00130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HOR20221.1"
                     /translation="MSEKSMLDLAYEVISQAKELPISFHELWEAVCIKAELTKEEMRA
                     QISRFYTNISLDGRFVALTDNFWDLRSHHTFDKVHIDMNDVYSEVEETEEIDEEEIEY
                     LKIEKDDDKDELEEEVEEVEDEEKVKIEDLY"
     gene            complement(16038..17660)
                     /gene="argS"
                     /locus_tag="PLH17_00135"
     CDS             complement(16038..17660)
                     /gene="argS"
                     /locus_tag="PLH17_00135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOR20222.1"
                     /translation="MNIETFLKNKILHAIKKIGLDIQEGEVVLEHPKDETRGDYATNA
                     ALQHAKRIGLKPRDLAAKIVEEIDCSGLVKIEIAGPGFLNFFIASNTLQAIVEDIISQ
                     DSNYGRGMPKKRKINVEFVSANPTGDLHLGHARCAAIGDSICRLYEFAGYEITREFYV
                     NDAGAQVNKMGASLRARYLNEFGIDASVPEDGYHGLDLVDIAKLIKEIHGDKLVADTE
                     ANMKTFIQEGIRLELEKIKKDLADFRVTFDLYSFETDVRKDGKVEKTLQDYREFTYLD
                     NGATFLRTSAFLDDKDRPVVKSDGSYTYLMPDITYHLEKLSRGYDLLVDVLGADHHGY
                     TNRMRSALMMKGYPAEIIDFELVQVVRLMKGDVEVKMSKRGGTTITLRELSEEVGVDA
                     TRYFFVARAATSHLDFDYDLALERSSSNPVYYAQYAHARLCKLLETGADIGLDISAKN
                     LRESSEINLLKHLQEFPITIESCAKTREPYKVATYIQKLAGLTHAFYTECRVIDRDNP
                     ELSQSRLALCKASQIVMRNALNVLGVTAPEQM"
     gene            complement(17704..19026)
                     /locus_tag="PLH17_00140"
     CDS             complement(17704..19026)
                     /locus_tag="PLH17_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOR20223.1"
                     /translation="MTRLRFAHQDGGHLNLSQLRKAFLTYLISRKLRGSFIFRLDNVM
                     RSEEDGMTTNLFALEDLDWLGLIADESPLNPNPGFSPYVQNERLDIYQRYIDALLAVG
                     AAYKRVPPKEKGSPLGAFRPATYFKMRALGETKINDLRIGKVTDDKLNLNDWIIAGSN
                     GLPSEEFAAIIDDHLMGISHVVFELTNLPNLGRYVALNRALNWQLPTFIHISIGKTNR
                     DFDEKILNLLKKNGYLPEAICSYLYRLDVDDNKADYRMNMEQIVNSFEIDNIRTDHQL
                     FDEKILRKINSVFIKTMKTSDYTAFMRPFIQPFISEEYSDDFLLKLALVFQNQISYGR
                     EIEEFIAPFLLQDPDFTPREQQIISNPQAQSVLSSFYERLANCDDDLTNVASLLDETQ
                     KKTGIAGKAFYQPLRLLLTRMEKGPELEEIIKFLGKAETLRRLSQDKD"
     gene            complement(19033..19509)
                     /gene="ispF"
                     /locus_tag="PLH17_00145"
     CDS             complement(19033..19509)
                     /gene="ispF"
                     /locus_tag="PLH17_00145"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOR20224.1"
                     /translation="MYRIGYGEDIHRLVAGRKLILGGVEIPFEKGLLGHSDADIVIHA
                     LMDALLGALSLGDIGTHFPDTDKQYKNVDSMELLNKVNRLIEENGYAVKNIDISIALE
                     KPRLSPYLSKMVANLARSLRIMDRNVSIKAMTNEGLDAVGRGEAARAVAVVMLQRK"
     gene            complement(19521..20627)
                     /gene="ychF"
                     /locus_tag="PLH17_00150"
     CDS             complement(19521..20627)
                     /gene="ychF"
                     /locus_tag="PLH17_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOR20225.1"
                     /translation="MPLKAGIVGLPNVGKSTLFNAITNSQVEAANYPFATIKPNTGVV
                     VVPDERLDFLSNLFKPLKTIHATFEFYDIAGLVRGASKGEGLGNQFLAHIRECDAIVQ
                     VVRCFESNEIIHVENSIDPQRDIETINFELALADLDTVDKRIAKVENKARLAKEKDAV
                     YEMAILAIIKENLNRGISARNAPLSSEQISYANKNFHLLTLKPLIYVANVSEEDYADL
                     SIAKHYQEVVRIAGQEKAQVIPISCEIEYQLSLLSKEDRREFLKEMGASESGLDKVIR
                     ATYRLLDLATFFTVGADECRAWTYKNGSSASQCAGIIHTDFQKGFIKAEVYSYEHLFH
                     LGSELAVKEAGKLRIEGRDYLVKDGDIMFFRFNV"
     gene            complement(20752..20949)
                     /locus_tag="PLH17_00155"
     CDS             complement(20752..20949)
                     /locus_tag="PLH17_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOR20226.1"
                     /translation="MKGTVKMFNKEKGYGFIHTDEGSDVFFHYSSLVMEDYKTAEVGE
                     KVEFDVEESARGLRAANIKKI"
     gene            complement(21026..23860)
                     /locus_tag="PLH17_00160"
     CDS             complement(21026..23860)
                     /locus_tag="PLH17_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20227.1"
                     /translation="MIRLNHIIKEYGTRRVLNRITLRFPKTGFLAIVGPSGCGKTTLL
                     NILSGIDEKYQGDYLFKNKSVDYLTREQKSAFRLLNVGYVFQDFRLFENDSVMNNLLL
                     PLDVLSNDEAEFKKRHVSSLLDLVGLSHKKDVAVNLLSGGEKQRVAIARAMVNNPAFI
                     LCDEPTGSLDEGNANKVMDILKSISAQRLVIVVSHDEELVTRYCDDKVTIVDGMITGH
                     QKFERTAIKKKITFQQMKKANNKLHMPFKTMLRRALSIIKTRKYRVILNNSMMSLGLL
                     GIGLSIIMSFSIQERIVTGFSQVIKDGMIVMSKKGDETPITAYSASFENVSQIAETYG
                     SWINGIGVSYAVNFESFFQDRDEMFISSTAYKIILPRFSTRQINDFLWLDEIGSNHQI
                     YPFQQASMDNDEIVIGLPHNEMVGVCYSLRIERNYASLGEYISGVRPLVTLGIANGDW
                     GYGDEQVFHLSGVVPSDAPAIYHTNHLWSEYVFQEKMRIPSIDDGKYEFPWQMAKSYF
                     VTTKENPETFLEVATLEKPLRDFVFEMAGFSFHPTHCPMSGPCLLKRLLVFHVDKDTI
                     DISALDGMMKKEHQIKNHLYATDGGYYYHPNSFLAGFAKNIFISLSDTQIEKAIDADS
                     LIASENDNMIFEPPPGVFSGNIQTSMDGGVSFSSDLREVIYGRKPINLDEIAISETLA
                     HKFLSPVEMVGKKLLIATNYANIAYDNDRIEKQYSLTETTIVGIIRGSGITLHHRPYW
                     SLGFFQLKGGVSAFELIPKHVIFEIDKNGDSELLVEKLNKQYGEFDFYDPMKTISDGL
                     DETMSFIRVVLFAFSSLAVLTSLFLFFIVMFVTIEENRRDIILLDYLGISKSAIRRSF
                     IVCGLVVSSLSFFLSGLEIIILDYFVTHVIAGFVGTNIPYVFDYRPLAAILAMSVVIA
                     VAASYAAFEKQYSSLKKQFIKVKNFAKNNR"
     gene            complement(23863..25245)
                     /locus_tag="PLH17_00165"
     CDS             complement(23863..25245)
                     /locus_tag="PLH17_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20228.1"
                     /translation="MIISNWKEKIARSYIWGDASKNIILFLSTLISFASLLLSVGFYY
                     GANQVVDTTQERIVDSALFKATKKERISSGDAPLSLIKVTRPRRDDLSFLSGIVSSAI
                     VTDDFTKVFPGNHDLFFHDKRIDDPVFSPVYSYLHLRQKSELLIEGMIPNQETMEEVL
                     INKTFATILSTDFHNLVGQKLTIYIKTKITIDDEYNSVIEDVFLWQKEMMISGIFEEL
                     SFLNTPKIFYSHVAFEELLENYYLEDISDHFNRDISCLDIFRIIADNHYLTNYDLNVF
                     VLSKDDLPVVSEISKKLESSGSNLALESSSLLIKNTYQELTKAAFYSMLVFLVIALVG
                     TCSIMAIGAYSNYVSKKKESAILTSIGARKNDILDIFLYKSLFIIFAAIIAAFAFAIF
                     AQKIANEYFYKHFMFKNLIDIPLAAFMGRPFFLIFIVLSSGYVITIVFTYLPLRLYKG
                     LSLADELREN"
     gene            complement(25242..25940)
                     /locus_tag="PLH17_00170"
     CDS             complement(25242..25940)
                     /locus_tag="PLH17_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20229.1"
                     /translation="METPILKIDNLSRVFSLGGQDYPVLQNIDLAFEERGFVLLSGKS
                     GSGKSTLLNLIAGILKPSHGHVLYKNKTIDGMNEKHICAYRNREIGFVFQQYNLFDKQ
                     SVLFNVVLPLLIDGRGISKSYETGRKLLGAYKLDSMHDVEVGKLSGGEKQRVAILRAI
                     INDPAIVLADEPTGALDRENAIKTLEMLKAISKERLVIVVSHNLDLALAYATRHIEVK
                     DGQIVSDVSFPEQS"
     gene            complement(25978..26196)
                     /locus_tag="PLH17_00175"
     CDS             complement(25978..26196)
                     /locus_tag="PLH17_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015638459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOR20230.1"
                     /translation="MLDYQLFDIVEMKKEHPCSARTKRFQIVRVGADIKMMCLGCGNV
                     IMLTRYDFNKRLRKVIAHEEKAIVIKNN"
     gene            complement(26204..27820)
                     /locus_tag="PLH17_00180"
     CDS             complement(26204..27820)
                     /locus_tag="PLH17_00180"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOR20231.1"
                     /translation="MPLLQIDEIAKIAGIPLTYIEPYGKFKAKIDTSIFDVLKDVPDG
                     KLVLVTAITPTKAGEGKTTTSIALAEGMAKINQKAILSLREPSLGPVFGIKGGATGGG
                     LASIDPRDDINLHFTGDMHALTSSINLIAAVIDNHLYQGNELRINPQRIVWRRAMDMN
                     DRALRDIIIGRGEKNGIERQDGFVITVASELMAILCLAKDEEDFRERVQKIIVAFNVD
                     EEPIRVRDLKISHAIMKLMKDALKPNLVQTIENNPVLIHGGPFANIAHGCNSLIALKL
                     SRKLAPIAITEAGFGSDLGAEKFFDIACRYGEMKPDGVVMVATLRALKMHGGQLLEKI
                     SEENVETLLKGVNNLRVHLENIKKFGVPSVVAINHFASDHQSEIEALEKWCEEKGYVV
                     SFLDGYLKGGEGAVDLAQKVVKMLDTEPSHYKPLYKLDLSIEEKIEKICKQIYRAGKV
                     EYSKFAREQIAKFNQLGYGALPVCMAKTPLSLSDNPEVIGAPEGYTITIRELGLSAGA
                     GFVVALTGNILTMPGLPKVPSAVLMEDLPY"
     gene            complement(27867..28550)
                     /locus_tag="PLH17_00185"
     CDS             complement(27867..28550)
                     /locus_tag="PLH17_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOR20232.1"
                     /translation="MEKRLERFAALVGEDKVKALRDKSVLVCGLGGVGGTALESLARS
                     GIGHFTIVDYDVVEISNLNRQILFTNKDVGKPKVEVAKERVQTIDESIIVTAFDCRVD
                     DNFLTRISGRKFDFVIDAIDAPEAKVQLIKHCLANNLLFISSMGMANRLDPTAVRIAN
                     LAKTDTDPLAKKMRMLMRREQIDLADIPVVYSLEKPLRKGSLLASVMNVPSAAGLALS
                     SYCLEKIQK"
     gene            complement(28534..29412)
                     /gene="miaA"
                     /locus_tag="PLH17_00190"
     CDS             complement(28534..29412)
                     /gene="miaA"
                     /locus_tag="PLH17_00190"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOR20233.1"
                     /translation="MIYVITGPTNTGKDRIAIELAKRLGGEIINADAFHVYRQLHIGT
                     NKPKEEDFQGVRHHLFAIVDVDENYSIARYQKEARVIIDDLLSKNIPVIMLGGSGLYI
                     KAALFDYRFKEEAEVDLSQYDSLDNQALHNALKVIDAKAAETIHFNNRRRVLRALQIY
                     RSQGIKKSEIPLKKDSEPLYETLFFGIEFERADLYLRIVERVSRMVENGLFEEVRNLI
                     NEYGVKQQAFHAIGYKEPISYLLGKTSREQSINDIITNTRHYVKRQMTFARHQFKMHW
                     IKSVDDIIEISHGKTS"
     gene            complement(29449..29760)
                     /locus_tag="PLH17_00195"
     CDS             complement(29449..29760)
                     /locus_tag="PLH17_00195"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOR20234.1"
                     /translation="MNNIENALDNLEREIMNSELIKEYFRLRALIGENEELKALKIKV
                     NAAQVALSLSMADEQEHALKKAEYENLLAVYDNHPLVANFTSIQSEVHNFLKNIADLL
                     D"
     gene            complement(29753..31195)
                     /gene="miaB"
                     /locus_tag="PLH17_00200"
     CDS             complement(29753..31195)
                     /gene="miaB"
                     /locus_tag="PLH17_00200"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOR20235.1"
                     /translation="MAKPKILSKPDLDELRIRQKLMPVHLYEPFVSDLVRDFAKNKRY
                     YIRTYGCQANIRDEEVISAILERAGFVRAASAKSADFIMLNTCAVRENAEDKVLGELG
                     TLKALKSANKDLIIAVSGCMSQQEHIIDLLLEKYPQVDLILGTHNIHKIIELIDIHLR
                     ESCRVVDVLARPQEIVEKLPSTRKAQHKAFVNISYGCDKFCTYCIVPYTRGQERSRPF
                     DEIVAECRHLVERGYQEITLLGQNVNSYGKDFKNRNVTFATLLEAVALLGISRLRFLT
                     SHPFDFSDDIIEVMKRHENLMKCVHLPVQSGSNEILRSMGRRYTREQYLEIVQKLRDQ
                     LPGIALSTDIIVGFPNESEEQFMETVSLIKEVKYESAFTFIYSPRKGTPAAKLIDNVS
                     KKEKAARFKILTKALEETIAESSYGMVGNIYEILVDGWSKKDKSVLSGYSETNKLVHF
                     RGEASHIGKIVRVRILESHTYSLIGELVDE"
     gene            complement(31188..31985)
                     /locus_tag="PLH17_00205"
     CDS             complement(31188..31985)
                     /locus_tag="PLH17_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOR20236.1"
                     /translation="MRILFFGDIVGRNGRQVVKRHLGQLIDKYRIDFVIANGENATHG
                     KGLIEKHYHELIDAGIDAITLGNHYATKSDIRRYIDEAECLIRPLNILKSMGGSGSIV
                     FDVKGVKIRVTNLLGTAFMKEEVNHPYYALKDLLAETPEDEIHIVDFHGEATAEKIAF
                     AFAFDGQVSAIIGTHTHVQTRDYRLLPNKTAFISDVGMCGAYNGVIGFKKEEVINRTL
                     FGKDDIFQLDEGDQSLLSAVVLDIDNFTRQAKEIFPIYIVESGRNDG"
     gene            complement(32012..>33445)
                     /gene="rny"
                     /locus_tag="PLH17_00210"
     CDS             complement(32012..>33445)
                     /gene="rny"
                     /locus_tag="PLH17_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOR20237.1"
                     /translation="IRDAEIKAEHTVKSAQIDAKQVAYEMKLETEKELKERKAEYLVL
                     DNKLSQREANIDRRDAQLITKENVLEEKTETLNRRLKEAERKEEVLQQKIDSIIQELE
                     KVAQLSINEAREEIFQRVESKLSKEISAFIKNKEDEAKETAQEKARELIGLAINKYAQ
                     EVTTERTVSAVSLPSDEMKGRIIGREGRNIRSLEQLLGVDLIIDDTPEVITVSCFDPI
                     RREIAKLTLERLIKDGRIQPGRIEEVVEKVKVEVDESIRKAGEDAAFKLGLPRINKEL
                     LTYVGRLKYRTSYGQNAYDHAMEVAYLAGIMAVELGLDQNLAKRAGLLHDIGKSMDFE
                     IDGSHVEIGVRLAKKYGEPEVVINSIESHHGDVEPKFVISHLVAAADTLSAARPGARS
                     ETVENYIKRIEKLESLCKSYDGVYQSYAMQSGREVRVMVIPDKIDDLAAYKLARDIRE
                     KIENELTYPGQIKVSVIREYRAVETAK"
     assembly_gap    33446..33545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33690..34688)
                     /gene="recA"
                     /locus_tag="PLH17_00215"
     CDS             complement(33690..34688)
                     /gene="recA"
                     /locus_tag="PLH17_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOR20238.1"
                     /translation="MAKETSKDKMSALDEALKAIEKTYGKGAVMRLGERPHVEVDAIP
                     SGSLLLDYSLGVGGYPKGRIIEIYGPESSGKTTLALHAIAEAQKMGGRAAFVDAEHSI
                     DPIYARNLGVDIDDLILSQPDSGEQALEIVEMLASSGAIDIIVVDSVAALVPQAELDG
                     LMGDNQVGLQARLMSKAMRKLVAVLNRSSCTIIFINQLREKVGIVYGNPETTSGGRAL
                     KFYASVRIDIRRSEAIKDGNDLVGNTVNIKVVKNKVAPPFKSCKVDIIYGKGISKEGE
                     VLDLAIDMGLIKKSGSWYEYSGERIGQGRETAKEFLKKNETVMHDLVTKLKTGIAQ"
     gene            complement(34739..35203)
                     /locus_tag="PLH17_00220"
     CDS             complement(34739..35203)
                     /locus_tag="PLH17_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOR20239.1"
                     /translation="MKAQNLVEILKSKNLTIGAVESMTGGLFASAVTDIPGASDVFCG
                     SIVAYTPQVKCNLVNVRKETIDQFGVVSWEVASEMAYNGRTLLNVDYCIAVTGNAGPT
                     ASDSKKEAGSVFLAVASKDNIWGVPLNLSGSRTEIKKQATALMISTLETILK"
     gene            complement(35181..35828)
                     /gene="pgsA"
                     /locus_tag="PLH17_00225"
     CDS             complement(35181..35828)
                     /gene="pgsA"
                     /locus_tag="PLH17_00225"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOR20240.1"
                     /translation="MNLPTKITISRIVIVVAMLITLFILSLLNIGVPNLGNTGINLVY
                     FITAIVFILASATDWLDGFLARRNNQITDLGKFLDPIADKLLIDGLLIFLIVAPHYAP
                     STLTFSLWCAIILIARDLIVDALRLIAVTKNKVIAANIFGKAKTVLQMVAIVAILLNG
                     WPFSYFDSGFPEGLRVAELLVYLATAVSLLSGIIYVVTNRQVLREENKDESSKSR"
     gene            complement(35851..36181)
                     /gene="rnpB"
                     /locus_tag="PLH17_00230"
     ncRNA           complement(35851..36181)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLH17_00230"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(36185..36547)
                     /locus_tag="PLH17_00235"
     CDS             complement(36185..36547)
                     /locus_tag="PLH17_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOR20241.1"
                     /translation="MDFKAKITSKTILNKPFSKNVKGYDALEVDKFLDQVALDYLAFE
                     KVLLERDDYIAKLEILIKKHRDQTSALEIENAKYRKRLENIKDEGKVSIQNVEYIRRI
                     AALEKELYRLGFDPSKIK"
     gene            36671..37396
                     /locus_tag="PLH17_00240"
     CDS             36671..37396
                     /locus_tag="PLH17_00240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOR20242.1"
                     /translation="MIELKKAIRGRKHLVFLDLEGTQFSHEMIAIGAVRAVLGKDGTI
                     RKLYPGFKRLVLAKNNIGKVVVQLTGITNEMLKKDGVTYRVAMEDFKKYVGRSFTKSL
                     FITFGNHDLRIIAQSVSHNLDANKNDAKFIKENYLDLAAFISKYIKDEKGNSLSLDNS
                     LKIFNIEFDGTKHDPLDDARNLARLYDAMHINPNLLAQEYKKVLAKNKVLPAPLPKLI
                     NKLTKGESVTPDEYEDMIREYVS"
     gene            37398..37979
                     /gene="recU"
                     /locus_tag="PLH17_00245"
     CDS             37398..37979
                     /gene="recU"
                     /locus_tag="PLH17_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOR20243.1"
                     /translation="MIRYPDGSSPQPKREKAIKRPRALSSANRGMNLEEDINISNQYY
                     IDKDIAVITKRPTPINVVKVDYSRGAKITDAYFEKQSTTDYNGVFRGKYLDFEAKSTK
                     LHTSFPLSNITAHQILHLQRVIKQGGIAFFIIRFEALDETYLLDASFVIEAFSGEKRK
                     SIPYPDVKKNGHLIKKGFIPRLFYLEVVESVYF"
     gene            complement(37965..38603)
                     /gene="nth"
                     /locus_tag="PLH17_00250"
     CDS             complement(37965..38603)
                     /gene="nth"
                     /locus_tag="PLH17_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOR20244.1"
                     /translation="MKNSEKILIFKKYLDDNFPHAQCELIYQKDYELAIAVVLSAQTT
                     DKAVNLVTPILFSKFNSLEKLKEASLEEIGEAIRSIGLYRNKAVSVKGIAAMLADEFG
                     GRVPADSASLLRLPGVGNKTRNVIQAELFNIPSMAVDTHVERISKRLGFAKQKDNPDQ
                     IETKLRKLLKPEDLVKTNHQIITFGREICHARKPKCDKCDLYSICNYFSKIN"
     gene            complement(38593..39252)
                     /locus_tag="PLH17_00255"
     CDS             complement(38593..39252)
                     /locus_tag="PLH17_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOR20245.1"
                     /translation="MDNISMEALDFRYLLIEHYKKIGLDENELSVILVIDHLLEQKNK
                     FITADLLGLRMTLKTEVIDKILVRLITRGLLEYETTNKNIKTSLKPLRTKLYKEFQLA
                     LAKEQETNSSAARNATLKNIYEVFEKELNRTLSPLEFSIINEWVNFGYSDEMIINALK
                     EAVSKNKRSLRSIDKILLQWQTRDDMEKEGYSAVSEKWNKDIEQTIKIAKTKWVDDDE
                     K"
     gene            complement(39303..39908)
                     /locus_tag="PLH17_00260"
     CDS             complement(39303..39908)
                     /locus_tag="PLH17_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20246.1"
                     /translation="MDKSIFINLARLFEKHGFSLFMIGGTSRDYLLEREVLDYDFVTD
                     ATPEEMKNFLDAASYTFAKFGTISLFYNKQKIDIVTLREEADYIDFRHPKTIRYVRDI
                     KRDYIRRDFTINAIYIDKDFKIYDFVDGLKDLKEGIIRFIGEPEKRVIEDPLRILRAE
                     RFARKLGFIIEEKSLAAMNKYRHLLAKLNPDKVREEENKGK"
     gene            complement(39982..40254)
                     /locus_tag="PLH17_00265"
     CDS             complement(39982..40254)
                     /locus_tag="PLH17_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOR20247.1"
                     /translation="MNKADLILAVAEEADLSKRQAEAAVESFLKLVEKALVKKQAVKL
                     SGFGVFENKKRAARTGTNPTDGKKIQIPASHTVSFKPSKSLKEKVN"
     gene            complement(40324..41325)
                     /locus_tag="PLH17_00270"
     CDS             complement(40324..41325)
                     /locus_tag="PLH17_00270"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOR20248.1"
                     /translation="MRIGIAGTGTWATALAQVLTDNGHKVILYGREQNQVDDINLNHR
                     NSFYFGDLINLSPSIVATNSIQDICRDSDLILLSVPTGAVSDVLIKMLPHLSKKTLFV
                     NTAKGFDPDTKERMSEVIRHHISKDKLAGVVSLLGPSHAEEVILRHLTLITAVSRNKK
                     YAELVQKLFSNRYFRVYILKDEIGAEYATAMKNCIAIAAGIIDGLGLGDNAKAALITR
                     GLNEMVKLGKHYNARLKTYLGLTGIGDLVVTCGSKHSRNYQAGFMIGSDDSSQRFHRE
                     NIKTVEGVRTAKVIHELASSSGLSLPIVEAIYKVLFEDAKPSKVITELMSRPLKKED"
     gene            complement(41328..42638)
                     /gene="der"
                     /locus_tag="PLH17_00275"
     CDS             complement(41328..42638)
                     /gene="der"
                     /locus_tag="PLH17_00275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOR20249.1"
                     /translation="MPLGVVAIIGSPNVGKSTIFNRIIGTRRSIIDDEPGVTRDRLYA
                     DATWLTKEFRVIDTGGIEIANRPFQEQIRAQAEMAIDEADVIVYIVDGQVGVTNDDQE
                     IAKMLYKSNKPIILAVNKIDDISKIADIYEFSTLGLGEPIAISGAHSIGVGDLLDQIV
                     KLLPESEPEKREENEIVFSIIGRPNVGKSSLSNAILGQNRVIVSAIEGTTRDAIDTKF
                     IRDGKQYVVIDTAGLKKRGKIYEAIDKYAALRALSAIERSEIVMLVIDAEQGITEQDK
                     HVVGYAVELNKAIILVVNKWDLVPRSQTAMSDFTKKIRKEFKFLEYAPVIYVSAKDKS
                     RIDTVFQALDIVYEAYGRRIQTSVLNDILQDAQIMNPAPLFEGGRLRIYFANQVDARP
                     PTFVLFVNRPKYAHFSYLRYIENRLRESFSFDGTPIKIILRERK"
     gene            complement(42643..43311)
                     /gene="cmk"
                     /locus_tag="PLH17_00280"
     CDS             complement(42643..43311)
                     /gene="cmk"
                     /locus_tag="PLH17_00280"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003183032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOR20250.1"
                     /translation="MKKVTIAIDGPAAAGKSTVAKKVSQALGYTYIDTGAMYRAFTYY
                     VMKKGLDPKDEAASISLVPEVDIELHSDGRVICCGEDVTKVIREPQVSANVSYIASYK
                     DIRLALVELQRKMAQRRSVVMDGRDIGTYVLPNADVKIFQIADVPTRAVRRYEENISK
                     GIKCTLAEVEEDVRRRDYIDSHRDFAPLKPAADSVLLDTSFLSIEEAVEAVLDIIKRK
                     IGEA"
     gene            complement(43326..43667)
                     /locus_tag="PLH17_00285"
     CDS             complement(43326..43667)
                     /locus_tag="PLH17_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20251.1"
                     /translation="MKALTRVEKFQKLRDEISRMPDDSELSSDQKPVGYEIAQTKKSQ
                     VSDIKSARSTIEFGIDDIIESVRASDENERKSVSPIVKQKRKELIQTIILISILILLI
                     VGLIIIGINAF"
     gene            43793..43978
                     /locus_tag="PLH17_00290"
     CDS             43793..43978
                     /locus_tag="PLH17_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOR20252.1"
                     /translation="MAKKVRIDRDACIGCGLCVSVMPEVFEFDDEGKARVHAQPAEGV
                     EVEVANDCPTGAIIVED"
     gene            complement(44005..44709)
                     /locus_tag="PLH17_00295"
     CDS             complement(44005..44709)
                     /locus_tag="PLH17_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOR20253.1"
                     /translation="MGAAVVGLLLIWIFLLIIVLREGKTFHFQKIDIIRFMSRVAIFG
                     AISAILYIVPFLKFPLPIFPSFLEFHFDEIPLFIAGFAYGPFSAFCAIIVKTIIKLPF
                     TSTLTVGEWADLIYSTAFVIPAAIIYQRKRNIKGVILGFAVGFIVQVATSLVFNVYIM
                     IPFYMFVMGFPEAAILAMSKAANPAVTDIGWSLGFLAIVPFNIIKNAMVVILTFLVYK
                     SIHRFMAQFEQKKLPG"
     regulatory      complement(44739..44852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(44905..45711)
                     /locus_tag="PLH17_00300"
     CDS             complement(44905..45711)
                     /locus_tag="PLH17_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOR20254.1"
                     /translation="MKKITKDELLPLPKRSKQTNKYDYGNIVIFGGSIGFFGAPALSA
                     LAAYRTGSGLVSIALEEDDYSLYVNLNPEVMIKRFIDIEEVEKTLENKNAVLFGPGLE
                     DNLMNERILKLLLRKNIPLVIDATGLTILKKVGLNKRLDHVILTPHMGEAKRLLDSHE
                     PHKEIHTLTSLGATVVLKDAVTTVYAKQYEGQLDFGNPGMAKAGSGDVLAGIIVSLLG
                     QNYTFLEAAKYGVYLHQIAGGIACERRGEHSLLASDIIDSISEALLRSGK"
     gene            complement(45726..46370)
                     /locus_tag="PLH17_00305"
     CDS             complement(45726..46370)
                     /locus_tag="PLH17_00305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOR20255.1"
                     /translation="MAIGQINPTRMELTKLKNQLAITRRGHHLLKDKQDELIRQFMEI
                     VYRTKDLRTEVETMTSKALSSFKKGRAKTREAELYELTSVPAFSWSLQTSKSMTMSVE
                     SPHLRFSLDGKTELPYALSSAPQEFDEALMLGLELLPKLIALAELEKTTETFAREIEK
                     TRRRVNAIEHIMIPEQVALIKDIKTKLADQEMAGTIRVMKSKEMIIKKIRNNQK"
     gene            complement(46373..47752)
                     /locus_tag="PLH17_00310"
     CDS             complement(46373..47752)
                     /locus_tag="PLH17_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOR20256.1"
                     /translation="MAIKQYKTIQEVVGPLMLVEKVQDVKYDELVEIKLANGETRLGK
                     VLEINEDKALVQLFESSQGMKIDDAKATFLGHGIELGLSPDILGRVFDGLGRPIDNKG
                     AVIADTRLDINGSALNPVARDYPSEFIQTGISAIDGLNTLVRGQKLPIFSGSGLPHSK
                     LAAQIARQARVLGKSEKFAVVFAAIGITFEEADYFIEDFRKSGAIERTVMFVNLANDP
                     AIERIATPRMAITAAEYLAYQLNMHVLVILTDITNYAEALREISSARKEVPGRRGYPG
                     YLYTDLASLFERAGRIKGIQGSITQLPILTMPEDDKTHPIPDLTGYITEGQIILSRSL
                     FMKGIFPPIDVLPSLSRLKDKGIGEGKTRGDHADTMNQLFASYARGKEAKELSTVLGD
                     SALSDIDKLYARFAEDFEAKYISQGFTTNRPIKETLDIGWELLRIFPTSELKRISDEN
                     IAEFYGTGE"
     gene            complement(47755..49518)
                     /locus_tag="PLH17_00315"
     CDS             complement(47755..49518)
                     /locus_tag="PLH17_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOR20257.1"
                     /translation="MEKIGTITKVAGPLVVASGMENVQVYDVVRVAHKKLIGEVIELR
                     GGLASIQVYEETAGIGPGEPVFYTNKPLSAELGPGLIESIFDGIQRPLTNIYDSFGPH
                     IPLGIDLPRLNREREWDFVPVHQLDVQVSGGVILGTVQETPVVNHKIMVPPSVSGKLV
                     WLHKGKAKVTDVIAKIVDNNGKTIELTMIQYWPVRKKRPYLNKIAPNKIMVTGQRVID
                     ALFPVSIGGIAAIPGPFGSGKTVVQHQLAKWADADIIIYVGCGERGNEMTDVLMEFPR
                     LKDPRTGESLMKRTVLIANTSNMPVAAREASIYTGITIAEYYRDMGYKVAIMADSTSR
                     WAEALREMSSRLEEMPGEEGYPAYLSSRLAEFYERAGYVACLGDKNRRGAVTAIGAVS
                     PPGGDTSEPVSQATLRIVKVFWGLSAQLAYRRHFPAIDWIKSYSLYEDVLHLDIDSHY
                     HKDWSKMIRRVQVLLQEEASLQEIVRLVGIDSLSKSDRIKLLASQMIREDFLHQNAYH
                     EIDTYASIAKQHRMLKTILRWYDLAKKALEGRKAFSEIEKMKTPERIGRIKYVREADV
                     EKECDSIEQAMEKEFSSLREE"
     gene            complement(49532..49840)
                     /locus_tag="PLH17_00320"
     CDS             complement(49532..49840)
                     /locus_tag="PLH17_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOR20258.1"
                     /translation="MNKRAVAIAKTENVFLFRSLGFETFVIDGEEKMKSVLESIAAEA
                     QIIIIDEDLQAMIGEYRKRFNEKAFPIIMALPIDHEASGQGLEKLRADVEKAIGLKLF
                     "
     gene            complement(49837..50781)
                     /locus_tag="PLH17_00325"
     CDS             complement(49837..50781)
                     /locus_tag="PLH17_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOR20259.1"
                     /translation="MMTDYTFASALLSTKSASLLNKAQFRELRNADDDGFILKLQSFG
                     YGVGEQPKAVEAIVGAEVLKLKRELSEIMPNDDLAPYFFTKYDLTNIRAYYKKKLFQA
                     ELGPFEPTGFLTEKELSQAILKNDYYNLQEPYKHLISEANNQTFSDSHSLVTFLEKTF
                     QKLMYEQIKSRQDASLEKYFVISTDINNLITLLRSRKLRSDAEQVKNNLLDCGSISIA
                     DIVHLVEASDREIASFYATAYLTRFSEPLDLFFEDGNFSSLEHALLRFLVDELTRDAV
                     DIKSSANIIIYVIRKQIELIDIRRIHLDRASAAMVDET"
     gene            complement(50778..51398)
                     /locus_tag="PLH17_00330"
     CDS             complement(50778..51398)
                     /locus_tag="PLH17_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20260.1"
                     /translation="MSIYDKIINKGRAESDSIRQDAERESKAIEHTIVAEAEREAALL
                     LAKADESKTTSIAQAEALSELEKRQSVAALKNKSIDEIFSAVLDHFKSLKGSDLLSFV
                     VKQIQDEKIKGDEIMRVNARDYERYRLALSSQDLAHNVVLDRLNKALGKDYHLVLEDV
                     SSNEEEGFLLLGDTYDLNFSVKPILDRLRKRKEKELFASLFENEGE"
     gene            complement(51409..51882)
                     /locus_tag="PLH17_00335"
     CDS             complement(51409..51882)
                     /locus_tag="PLH17_00335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOR20261.1"
                     /translation="MTYGLVLSIIGAALAAGLAGIGSAVGVSIAGRSATGVLAEKPNL
                     FGSVLVLQALPGTQGIYGFLMAVLILQKVGLLGGQAVPLDDWQGWAFLGAAIPIALVG
                     LLSGIAQGKVAATSILMTGKRPEMATRGITMTALVETYAILALLVSILMYAAVAV"
     gene            complement(51887..53821)
                     /locus_tag="PLH17_00340"
     CDS             complement(51887..53821)
                     /locus_tag="PLH17_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOR20262.1"
                     /translation="MIVPVKKARIFVLEERKDELVRALQKASLLMIVPNSSSSVDVTS
                     DTVLLQRINKAINDLSHYGKKKSLFKHHEVTYENFMLEDERQLRLLEDVETKIENLNS
                     LLSHIKKTEESFQRYAPFREIDINVSDLKKSSFSRYHVGHVQEDSLDNLKAFFAEKEI
                     PYQTFSSPIYGTALFFASFYEEDEQLLGEVNKRSFSEVDLPDVNLTMSDYVSNLENDL
                     SARKKQVGDLEIGLKDLAKSKSLLELMYDRIQAKIDRKSVAFAKTEKSFYLDGWVRAD
                     QVDELSKTVKAVTTDFDIELSDPTDGDLPPTAIKNNKFVTQFETITNMYSVPHHKELD
                     PNPAVAIWYWIIFGIMMGDIGYGLAMLTIFGLAIWLIKPKGTLKQLVSVFFYSGITSI
                     VAGILFGSFFGAPFDLGALIGSLFAQQWTTIILNPVNDPLIMLGFSLVFGVLHIINGL
                     GFKFALLIRRKDYVGAIADALSWILILVGLVLFAAQLVIWTSASLLSYIGLGFAGVGA
                     LLLLFLAGRKSKNIFGKVLGGLGALYKSTSYVSDILSYSRILALSLSSAVIASTMNLL
                     AGMIQGSVIGFILSLLVYLVGHVFNFVMSMLSAYVHDGRLQYIEFFGKFYEGGGYLFE
                     PFSIRLKQISEISDLREERR"
     gene            complement(53805..54125)
                     /locus_tag="PLH17_00345"
     CDS             complement(53805..54125)
                     /locus_tag="PLH17_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20263.1"
                     /translation="MEAMKKIIEAERQAASLVTEALVYKTMLINQTKKRAEEEAASLI
                     NEAEALGKKQEAEANAKIAEIKEKLANDLKEIEENIKKKTARKKDHLISQLIQEVTSS
                     DRSR"
     gene            complement(54233..54811)
                     /locus_tag="PLH17_00350"
     CDS             complement(54233..54811)
                     /locus_tag="PLH17_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR20264.1"
                     /translation="MVKYYFFTSQKDINAERFNAALVSSYKNIFSITFFDGRSGYVLS
                     DEHLYDYLESLIPVIMSDTDNSYQFLMSHDDSQVSRTAMKKMTKSRGVHLSTMADILL
                     NLALENDFELISLAKRQYAAISRPLMVTAEMFISCGLNASLAAKKLYVHRNTFAYRLN
                     QFIEATNLDIRDFHNALFFNIFTKLISNSRIQ"
     gene            complement(54853..55626)
                     /locus_tag="PLH17_00355"
     CDS             complement(54853..55626)
                     /locus_tag="PLH17_00355"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOR20265.1"
                     /translation="MTKIIGIANQKGGVGKTTTAINLSSALAHFKRRILLIDLDPQSD
                     ATRGLGFDSTLLSRSIYDVLANGYDINKVIKRTALKYLDLLPSSLKLASLDSTLSDKV
                     EDKTLVLRESLKNLKKEYDYIVIDCPPSLGILNLNSMAASDGILIPVQCEYFALEAVA
                     TTLAAISKVQRTSNPKLGIEGFLLTMYDSRTRLGTEITTQIRALFKENTFLATIPRNI
                     SIPESSSKGLPVTLYRPSSAGSLAYFSLAREVMDKEFIR"
     gene            complement(55667..56371)
                     /gene="rsmG"
                     /locus_tag="PLH17_00360"
     CDS             complement(55667..56371)
                     /gene="rsmG"
                     /locus_tag="PLH17_00360"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOR20266.1"
                     /translation="MNFEQFQDYCMNELNLTLNASQMGAFTSYLALLKEWNVMFNLTA
                     ITDEEKIIEKHFLDSLLPLKYVSIKEKSLIDIGSGAGFPGVVLAIANPQANIVLLEPN
                     NKKARFLQTVKDNLHLDNVTIVVGRAETQNALREEFDFAIARAVKQLNILLELAIPLL
                     KVKGLFIAMKSQGASEEIIASKKAMQILKAKIVVCHEDILPTDKDLRVNLLIVKNDTT
                     PLRYPRIYSQISSKPL"
     gene            complement(56368..58239)
                     /gene="mnmG"
                     /locus_tag="PLH17_00365"
     CDS             complement(56368..58239)
                     /gene="mnmG"
                     /locus_tag="PLH17_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOR20267.1"
                     /translation="MEFDVIVVGGGHAGVEAAHAAASMGCKTLLCTLNFKAVSRMSCN
                     PSIGGSAKGIVVREIDALGGIMGVAADYQYLQMKMLNTGKGPGVQCLRTQSDRNIYPG
                     RVQEILLNTSNLEIKEAMVTSLLHDNEKVFGVVMSDGAEIRAKAVVITTGTYMEAQIL
                     RGLEKTASGPDGEKASVGLSKNLTEMGIEIYRLKTGTPPRIRKSSIDFSKASVQYGTN
                     AKLAFSYTTKEFVAFENQAVCHLIYTSPKTHEIIRNNLERSAQFNGQIKGVGPRYCPS
                     IEGKITTFYDKPRHQLFLEPETKDGDSIYIQGFATSMPRDVQEQMVRSLPGFENAEIL
                     KYGYAIEYDAIVPTQFDATLRVKKYRGLYGAGQICGTSGYEEAAGLGIMAGINAVLAI
                     RGQAPLILKRNEAYIAVMIDDLVTKGAIEPYRLLSSRAEYRLLLRHDNADLRLTEYGY
                     QLGLIPEARYQSFLQKNESLNRVLSILRKTYLGANKKVNEYLLKIGCTPLKGGTLAYD
                     LLKRPDVAYSTMIELIPELIFHPLDETGIMQLEIMVKYEGYIAKQEKEAKSFAKFESL
                     LLPEDLDYLHIDGLALESRQKLQKIRPRTIGQASRVGGVNPSDISVLVLTLKRRGLL"
     gene            complement(58239..59642)
                     /gene="sufB"
                     /locus_tag="PLH17_00370"
     CDS             complement(58239..59642)
                     /gene="sufB"
                     /locus_tag="PLH17_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOR20268.1"
                     /translation="MTNMKNKLPQKEYEYGFKDEDVSILNTGKGLSEEVVRTISRLKN
                     EPEWMLEFRLKSYRAFLKLPMPKFGPDLAFLNFDVYTYFTRPSEKEESSWDEVPETIK
                     KTFQKLGIPEAEQKYLSGVATQYESEMVYHNMLKEVEEKGVVFLSTDQALKMMPDLFR
                     KYFNTVVPFADNKFSALNGAVWSGGSFIYVPKGVKLDKPLQSYFRINNERSGQFERTL
                     IIIDEGADVHYVEGCTAPIYSRESLHAAVVEIVVLRDGKCRYSTVQNWSKNIVNLVTK
                     RAICHDNAQMEWIDGNIGSQVNMKYPAVILSGDGSRGTCISIAVSDHGQYQDAGARMI
                     HLGKNTSSTIISKSIVRNGGVANYRGTVRHEKGALNSRSHVECDTLILDDISKSDTIP
                     TNQVFNDQSYIEHEATVSKINEDQLFYLMSRGMSEKDATQMIIMGFIEPFSRELPMEY
                     AVELNQLIKMDMEGSVG"
     gene            complement(59623..60069)
                     /locus_tag="PLH17_00375"
     CDS             complement(59623..60069)
                     /locus_tag="PLH17_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOR20269.1"
                     /translation="MPISLDPQMMRAIIMDHYEKPRNKRTPHDDRYQMMHIDSPNCID
                     DINVFLLEENGVISDCAFDGIACTISTASTDIMTELVIGKNVQDALYIIEQYQKMIHE
                     QPFDDTVLDEAIVFINTHRQAARIKCATIGWDALKELLDHDHKHEK"
     gene            complement(60053..61291)
                     /locus_tag="PLH17_00380"
     CDS             complement(60053..61291)
                     /locus_tag="PLH17_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOR20270.1"
                     /translation="MKKIDPYLIRDDFPMFKNKVMMQGKPLIFLDNASTTFKPYAVEK
                     AVNAYFQYETANSHRGDYDLCYNVDVKIDEARATLAKFINAEKREIVFTSGTSMSINL
                     IAYGYGTKYLTKDDEILITQAEHASNVLPWFKVGEMTGCKINYIPLNEDGKLVPENLR
                     KVISKKTKIIAVAHVTNVLGFIVDIKEITKIAHQYGAIVVCDGAQSVPHMPTDVKDLD
                     VDFLSFSGHKMVGPTGIGVLYGKYGLLEKTAPFMTGGGMNAKFDMCGDVTYLQPPSKF
                     EAGTQNIEGIYGLKAAADYLMSLGMENIRRYEDELRTYAIEKLKKNERVILYNEKAES
                     GIITFNTKGVFAQDEATYLNSQGIACRSGQHCAKVLLDYLGEVATVRASLYFYNTKED
                     IDAFVSALEKGGDFLDAYFA"
     gene            complement(61288..62013)
                     /locus_tag="PLH17_00385"
     CDS             complement(61288..62013)
                     /locus_tag="PLH17_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOR20271.1"
                     /translation="MANNIRKIILADCQKQELIVDFSEGFDGLEINVGKGANLALNLI
                     NFGESRNGKIVVKVEENAELFATMADFSNGNSRLDVAINLVGSNARGTWRLSTLASNQ
                     DDKKYAVHFHHLAQSTYGLMDNYGVARNSSRLAFSGANEIARGAKKSKTKQNAKIIVF
                     DEGAVAKADPRLNIDENEVEASHAAVVGKLSDDHLFYLMSRGLNLNQAKKLITYGYLL
                     PIANYFHDEAMKEKIAKIIEERV"
     gene            complement(62003..62830)
                     /gene="sufC"
                     /locus_tag="PLH17_00390"
     CDS             complement(62003..62830)
                     /gene="sufC"
                     /locus_tag="PLH17_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOR20272.1"
                     /translation="MDVLKIVDLHVNVEDKEILKGVSLTARSGETVALLGPNGHGKST
                     LLQAIMGNPRYRITSGQILLNDQDVLTMSVDERSKAGLFLGMQYPSEVSGVVNADFLR
                     ASINARRAKPLSLFQFYRLLDSTYKKMGIPFEMANRSLNVGFSGGEKKRNEILQMILL
                     EPRFALLDEIDSGLDIDAMTIVASAIREEQEKGRGFLVVSHYARLYNMINLNRTIVMV
                     NGKIAVEGGPEIIQKIDTKGYEWIKTELGIDIEKEEPAMSAVSIGVCATKEAIRDGK"
BASE COUNT        16491 a        14156 c        14378 g        17747 t
ORIGIN      
        1 cataacattc agcacctttt ttattttaat aaacgcgctt gattcgttcc ggattttctt
       61 ttttgatcaa gttcttgatg tcgtcgacgc ggtcgagctg ttcccacggc aatttcaaat
      121 ccgggcgccc gaaatggccg taagccgcta acttctgata tttaaaagtc ggcttgttga
      181 gcgcaaaatg ggcgatgatg gcgccgggcc taaaatcaaa cagctgacgt ataattttta
      241 aaatgaaatc gtcggtatat ttcgcggtgt gaaatgtcga gagattaatg ctcaatggct
      301 ctgatacgcc aatcgcatag ccgagttgaa tttccaggcg atcggcgacg ccggccgcga
      361 ctaagttctt ggcgacatag cgcgccatat aagcgccact gcgatcgact ttcgtcgcat
      421 ctttgcccga gaaggcgcca ccgccgtgtt tagcggatcc gccatacgtg tcgacgatga
      481 ttttgcgacc ggttaatccg gtatcgccca tcggccctcc tgtcgtgaag cgaccggaag
      541 gattaattaa aaaattaaaa tcgctattaa gaccaaatga tgcgacgacc ggcaccataa
      601 tttcccgatg gacatagttg atgaactttt ccttatcgta atccgggtcg tgctgaatgg
      661 acatcaagac cgtctccacg cgtatgtgat cgggatcgct atagtcgatt gtcacctgcg
      721 atttcatgtc cgggcgagca tggacaaatt tgccttcctt gcgaagcgcg ctagctttgc
      781 gaacgagctt ttgggctatc gaaataggga gtggcatata tcctttcgat tctttggtgg
      841 catagccaaa cataattccc tgatcgccgg cgccgatatc aagaaggctt ggtccatgcg
      901 taatcgcgcc agcgatatca agagactggt ttttgacgcg gacatcgatt gtgcaagtgt
      961 cggcgccgat acctagctcc gggctcgtat agccgacctc gcgaatgact ttgcgggcga
     1021 ttttttcata gtcgggagtg actttacatg taatttcgcc accgattaag acatactcgg
     1081 tcgtcgcgaa gcactcgcag gccaccctgg cgttttcatc gtgttccaga cagaaatcca
     1141 agatggcgtc gctgatgcgg tcgcacagtt tgtcgggatg gccttcggag acggcctcac
     1201 tcgtaaataa gattttttcg ttcatgatga gatcctttct aaataaaaga cttcccacta
     1261 aggaaagcct tctcaggaaa tcctttgact tatcgttcgg agcgtgggac tccgcttagg
     1321 aggagcactt actactttat cgtagagttg ctaccgtgat ttctcatcct tgtgtcacgg
     1381 ttcttgataa gacaaattat attgtcgtag ttattttaaa aaattcaact acccttcgtc
     1441 aagagcaatc cttaaattat tttataattt ccccgtaaag aggagcgttc atatcggccg
     1501 aggcgttagc cgcgttgctc tcatcggtat cgatgtggac ggcggtcgaa aaccgctcgc
     1561 tgacgcggac gacgacatct ttaaaaatga taccgcgttc gggagtgttg acttcaatca
     1621 tgacgatatc gccgcttttc acgccggcaa gagcggcatc ggcaggcgtg atgtggagat
     1681 ggcgtttggc gatgataacg ccttcttcga tctgaatgct tccgacgggt ccgacaatcg
     1741 tgatcggcgc gcttttggcg acatcgccgg attcgcgaat gggggcgatg actcctaatg
     1801 tgcgagcgtc ggtcatcgag acttcaactt gtgttttagg acgcaccggt ccgaggacga
     1861 cgacattttt aatcgtcgat ttgggtccga cgacatcaag tcgggttgta ctcacatatt
     1921 gaccgggttg tgacaaaggt tttttttctt ggagggcaaa tcctttgccg cataagattt
     1981 cgagatgttc gctggacagg tgaatatgcc gggcgcttgt ttcaattaat actttcattg
     2041 tctatttttc cttatataat gcttagttat tttaacataa ataataacga tttagcaaaa
     2101 aatcctttct ttctttattt taataataaa gtcggtttat aatggcgaag gggaggcatc
     2161 aaatatggaa aaagaaaatg tttcaacggc gttactcgaa gagttgatta aaaaccgcca
     2221 ggtcgccaaa ctccgagaac ttttcgagac gattccgacc atcgacctcg ccgaagccgc
     2281 caacgacatc gaagatattc gcgatattgt ctttatcttc aaagtggtca aaagcgatta
     2341 caccgccgaa ttcttcaccg atctctcatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn aatgaagatc agcgcctgac cggcatcatc acgattgatg atatcgtcga
     2521 cgtcatggag caagaagcga ctgaagatat gtcgaggatg gctttggtga cgccccttga
     2581 agactcttat aaagacaccg gcgtcttaaa gatggccatc aaaacaatgc cttggctcat
     2641 cgtcttgatg attctcggcg tcttctcgtc cgtcgtcctt tcttcttttc aggatgtctt
     2701 gtcatcgctt gccatccttt ctgtctttat acccgtcttg atggataccg gcggaaattc
     2761 cggaggacag acgattgcct tgatgattcg cggtatcgct gtcggcgagt ttggcccgaa
     2821 ggactatggc catattttat ggaaggaatt tcgcgtggct ttgctcgtcg gcataatggt
     2881 ttcgatcgca gctttcatca tctttatcat cgagatgtac ctcggtatcg tattttacgc
     2941 gcccgaagga attgtcggca cggctctttt attcgaaaga atgaagataa gcggtatggt
     3001 ttcgatcact ttatttttca ccatcgtcat ctctaaaacc ctcggtgtga ccattccgat
     3061 gatcgtcaaa gctcttaaaa aagaccccgc catcatcagt gcgcctttca tcacgaccat
     3121 cgtcgatgtt tcggctttgc taatctactt ctttatctgt acgcaaattt tccaattagt
     3181 gtaattgata tttgtaaagt atttatttat aattactctc gctgtcaaca aagagtgcgc
     3241 tgatggcgga atggtagacg cgtacgtttg aggggcgtat agggcaacct gtgtgagttc
     3301 aagtctcatt cagcgcacca atcaaccgaa aagatctcat gatatggggt ctttttattt
     3361 atgttattgc tattaagcaa aatgttggtg ctataatact gacgataaac gatgaaaaca
     3421 attcttcttg ccctcaaacg caataagttg atgacggcgc tggccgcctt tcttctcgtg
     3481 gccgtaattt taagcgccgc caccaattat ctcgtcgcta atcaggagcc cacttcagta
     3541 attcaaagtt ttcaaactct cgattctctt cctgatggtc aaggtcaaaa agtcaaagtt
     3601 attctcctca acggccaatc aaatgcctcg ggtgtcggaa gcgtccacta tctcgaagaa
     3661 aaatcgcaga gcgctgatta tgccagcttc gaggatggat atgacaacgt catgattaat
     3721 tttttcagtg aaaatggtaa ttattcttcg cacggggaat tcgtaaaagc gaaagtcggt
     3781 caaggttgca cacctgatta tttcggcccc gaactcggcc ttgccgattc gctcagtcaa
     3841 gttttcgccg atgaactcgt tatcctattg aaatacacat ggggtggttc caacttgcat
     3901 acgcagtggc gcgctccttc aatgccgggg ccaaccgggg acctctacac cgccttcatc
     3961 aatttcacat ccgcgcacat gaaatatctc ctcggcaaaa attacgatgc ctcaatcgga
     4021 gcgatgtgct ggatgcaggg tgaatcagat gcctatcttc cctatgcgaa tgactacaaa
     4081 gatggattaa gcggttttgt cactgacatc agagccgatc tcggggttta ttccggtgat
     4141 gacggaattc attttatcga tgccggcatt tacgatagtc ctctagttga tcaatacgaa
     4201 acgattaatc aggctaagcg gcatgtcgcg gctttatcat cgaaaaacca ctatttcgac
     4261 accattgaag ccgggctcga atacgacaag gaacccgctg ataatcccga tcttgctcac
     4321 tttgatgcgc tcagcacctt ggaactgggc cgtctgttcg ctaatgaaat cagcagcatt
     4381 tatagttaaa tctttgattt aaagagttaa tttctttacg ataatacaac tgttggttgg
     4441 tttcatttac aaccatctct ctagcaatgc aaccaacagt cgctgttttt ttcttatttt
     4501 tgcccctaca atgagggtgc taaacaagga ggacaaaaac catggaagaa agtttaggac
     4561 aaagaatcgc gagattaaga aggaacaaag gactcacgca ggaagacctc ggatataagg
     4621 tgggcgtctc ctcgcaggca gtctcaaaat gggaaaccga ttcttcggta cccgatgtga
     4681 ctctgcttgt aaagatcgcc aacatctttg acatcagcgt cgatgagctg ctcggcaacg
     4741 accgcatcaa agaaacgccg caaatcgttc ctccggcaga aagaaagcca attgataaga
     4801 tgattttaaa aataatcatc gattccgacg aaggagataa agtgcgcgtc aatctgccga
     4861 tcagcctcat caagattatg attgaaggcg gcgccaaaat gccggaagta aatggcaagg
     4921 atgtcttgaa aaacatcaac tgggataata tttttgcttt aatcgaacag ggagcgattg
     4981 gcaaattagt cgaaattgaa ggtagcgatg gcgttcacgt cgacatcgtc gtcgagtgat
     5041 gaaagtactt gtcaaaagcc aggaagataa catccgcgtc tggataccaa acttgttaat
     5101 caatccgcgc atcttgcgct tcatacttag aaaagcaaat gttgcggtcg atgataattt
     5161 aaagagagtc ctgccaaaac tttgcctgac gcttaaaaag tttcgccgcc atcacaaacg
     5221 ctttgttctc gtcgaagtta tcacccttaa aaaagaacac ataaaaataa ctctttagga
     5281 aaagtcatca agttcgtggt gataacaaaa agcgcgattt cctcgcgctt ttttattatt
     5341 cgataatcag cgatttctca ttcatctcgc tcggtttctc aagtccgaga aggtcgagaa
     5401 tcgtcggggc gatatttgcc agcttgccac ctttaggcat cagttttaag cctggtttcg
     5461 tgatgcagaa ggggacgata tttttcgtat gcgaagtgat gggacgaccg aaatcgtcaa
     5521 agacttcttc ggcgttgccg tgatcggcag tgatgagcat gacgccgcca ttatcttggg
     5581 tccagttata aatttcgccg acgcaggcat cgatggtttc gacagccttg acaaccgccg
     5641 ggaaaattgc cgtgtgaccg accatatcgc agtttgcgaa gttcaatatc acaacatcca
     5701 gatcaccctt gttcagctct ttgatcaaag catctttgac tttgtatgcc gccatctccg
     5761 gctgcagatc atatgtcgcg acttttggcg aattgattaa gacgcgccgt gaatttgcca
     5821 gggctggttt ttcgacgccg tcataattga cggttccgtc aaaaaagaaa gtgacatgcg
     5881 catatttttc ggtttcggcg atgcgcagct gcgaatagcc tttatcggcg agataaggcc
     5941 ccagaacatt gtcgagggtc ggaaggtgaa aagcgatttc tcctttgact gattcggcgt
     6001 acttcatcgt gcagacatag aagagatttt tgagaatgtg cttaggcgta tatggtttaa
     6061 aagccaaagt tccatctggt ttctgagggg ggtttccgta gaaatgggga ttggtgaata
     6121 cggtcgagat ttcaatcgcg cgatcggggc ggaagttcat gaaaataatg gcgtcgtcat
     6181 ctttgatgcg cccatcgaca ttgcgattat aatgcggcat gacgaattcg tcgctggcat
     6241 ccattccttt gcttggcaaa aaggcatatt gatctttaaa atactgttgg taatcgccaa
     6301 acgaaggtcc ttcgtgatcg acaataacgc gatagctggc gtcgacgcga tcgagattct
     6361 tgtcgcgatc catcgcccag tagcgaccgg aaatatcggc gatagcacca agtctcagcc
     6421 tatccatttc atcttggagt ctttgaatat atgaagctcc gatttgcggg tcgacatcgc
     6481 gaccgtccat aaaggcgtga acaaagactt ttttaagtcc gtttttcttc gccatctgga
     6541 gcattgcaat aaagtgttcg atgtgcgagt gaacgccgcc atcgctgacc aatccgagga
     6601 tatgaagggc cgagttatgc tttttgacgt gatccatcgc tttaagatat ttttcgttgg
     6661 cgaagaaagt tccttcgcgg actgccttat taatcaatgt cagcgattga tagacgatgc
     6721 ggccggcgcc gatgttgaga tggccgactt ctgaattgcc catctgtccg tcaggaaggc
     6781 cgacggactc gcccgaggtt tcaatttcgg tgtagggata tgatgcaaat attttgtcga
     6841 gattcggctt tttagcggca aagatggcgt tgccggcttc cgcgtggcga atgccgtatc
     6901 cgtccataat acagaggatg actggttttt tcataaatga acctttctat tcaaggcgct
     6961 ttttttcgtg aaagccaagc gcttttaaaa tgcggcgata agacattagt tttatccaat
     7021 aattataacc attaatgtcg gcgttgtaga tagcaatatg ctgtcttttc atttcgtcaa
     7081 gctcttcgag ttccttggcg aattgtgcga attcttcgct cttttttatc actttgttag
     7141 cttcggcaat ctcaaagatt gttttcgtta ccttttccag ttcgggagcg tattcaaaaa
     7201 tttcgtagtg attaaagagg ccgatttcct tattggataa ctcgttaatc cgatttgtca
     7261 cggcgattga aactttgatt ttctgatcga cgatcttacg ataaagagca agggtgatgt
     7321 cttttctttg agcgagtaaa agccgcatgg cctcccgcct ttttttaaga cgcttattaa
     7381 aattggagat aaaaagcaag acgataaaga aaaagaggag atagagcaac gccactcctg
     7441 cgacagctat taaagtccat tgcagccact cttgcatttt tttcttcgct aacaaataac
     7501 atttgttgct aaatcattct atcttactaa caaggaggtt gcaaggttaa gaggcataga
     7561 aaagtatcga cagcaacgcg gttttctcaa ggtggtaaca ttgacattta tattatccaa
     7621 tgataatatt ttgccgaggt gaaaatatgg acggaaaacc tatcatggcg atcatctacg
     7681 acttcgataa aaccctgact cccgaagaca tgcaaaatta cagttttatt cccgctctgg
     7741 gaatgactcc gcaagagttc tggggcaaga ccggtgagtt ctcggccaag accggagtcg
     7801 aacgcatttt gtcatatatg tatatgatga tttgcatggc caaagaaaaa ggcattaaga
     7861 tgacgagcga gtggctcaga tcgctcggca aggatatcaa gtattttgaa ggtgtgacga
     7921 catggtttaa ccgcatcaat gcttatggtc ttgataacgg cgtgaaagtt gaacattatc
     7981 tgacgtcctc tggcaccaaa gaaattatcg atggatgcag catcgccaag gaatttaaaa
     8041 tgatctatgg ctgcgaattc ctctttgatg acgaaacagg agagccgatc tggccgaagt
     8101 tcgccgtcaa ctatacgcaa aaaacacagc atttattccg catttccaaa ggggttatcg
     8161 aagcgaccga tgatgatcgt ttaaacgaaa agacaccggt ccgccgcatc ccttatcgca
     8221 atatcgtcta tttgggtgac ggaatgaccg acattccgat gatgattctc gtcaagcaaa
     8281 acggcgggcg ttcaatcgct gtctatccgc gcggcaaaaa agaaaaagtc gccaatcttt
     8341 ttgatgacgg tcgcgtcaac tacatatgcg tcgccgacta ttcttctaac agcgaactag
     8401 aaaaagtgat gaaacttatc atcgattcca tcgcggtggc ctcgatgctc gaaaaaaaag
     8461 aaaaatcaat tatcacgaaa aaggagtaat tatgaatatt gctatcattc tcgccgccgg
     8521 ctcgtcacac cgctttaatg ctgagatgcc caagcagtat tatgagatcg atggacatcc
     8581 attaatttgg tatacaattc atagttttca ccgcgtcgaa gcaattgatc tgattgttgt
     8641 cgtggcccaa aaagaccaca tcgactattt atcaaagctg gttcaggaca gcaactttac
     8701 taaagtaaaa cacataattt taggcggtaa aacgcgccag gaatcaagtt ttaacggcgt
     8761 caagttcgtc aaaactctag ctaaagatga cgatatcgtc ttaatccacg atgccgcccg
     8821 tccgctgatc agcgaacgga ttatcaccga ctgcatcgaa ggcgccaaaa aacataacgc
     8881 cgtaacgacc gtcttaccct ctgacgatac caccgttatt tcatctgacg gcgagcaaat
     8941 cggtttcatg cctgagcgca agaatatcta ccgcgtgcaa acgccacagg cctttaaggt
     9001 cgggatgata tacatcgccc accacttgtt aaaaaatgaa aacgacagca ccgatgatgc
     9061 cgggctcatc tttcgcacgt ttatgccggt ccatctcgtc atgggagata aaaaagcgat
     9121 gaaagtcaca tcactagagg atgtttacta cattaaaggt ttgctccacg gagaaaaaat
     9181 ctgatgcctg aagcatttaa agttggagat attatcgaag gcacgatcac cgagctgcga
     9241 aagttcggcg ctctgatgat ttttgaacac gatgcgacgg gattacttca catctcggaa
     9301 atcagcgacc gctttgtcta tgacatcgct cgctatgtcc aggtcggacg taattttaat
     9361 gttaaaattc tcgagatcga tccaaataac ggtttcatga aagtatcgct ccgcaaagtg
     9421 actattgaag atcgaatcga cttcaagaat tccagcaaaa aacggacggt tatcgccgcg
     9481 gaagaaatcg actttgcacc cctgcaggaa caacttgaaa aatggactga caaacaacta
     9541 gaaaatacaa ctgaggagga gtccgatgct caaagtaaat ctcgataatc tcatttcgcc
     9601 agtcgatttt gtttcttata aagaaaaagt tgctaccatc gatcgcgcca tcagcgaaaa
     9661 aaccggtgcc ggcaacgatt ttttaggatg ggttgacctc cctttgaact atgacaaaga
     9721 cgaatttgaa aacattcgaa aatatgccgc ggaagtccgc gccaattacg atgttttagt
     9781 cgtctgtggt atcggcggtt cctatctcgg cgcgcgagcg gcaatcgatg ctctgcgcgg
     9841 cttaaaaagc acggattctc tcgaaattat ttttatgggc caaactttct ccgccgaata
     9901 cactcatgaa attctcactt atcttaacga caagcgtttt gccgtcaatg tcatctccaa
     9961 aagtggcacg acgaccgaaa cctcgatcgc ttttcgcctc cttcgtaacc tgctcgagca
    10021 gaaaatcggc aaagcaaagg cccgccgcgc catatttgct acaaccgata aaaacaaggg
    10081 ggctcttaag tcactgagtg acatcgaggg ttaccgcact ttcgtccttc caagtgatgt
    10141 cggaggccgc ttttcggttc tcaccgctgt cggtttattt ccgatcgcct gcgccggaat
    10201 cgatattcaa gaattgatga atggagctat tgaagctcgc aaaatgtatg caaatcccga
    10261 tttatcaaat aatggatgct atcaatacgc cgtcgcccgc gatcatttgt accaacacgg
    10321 caaaaaggtc gaattatatg tcgtctacga gccaaaactt cttcaactcg gcgaatggct
    10381 caagcaactt tttggtgagt cagaaggtaa aaaccagcgc ggactctttc cggcttcggt
    10441 ctcgttttca accgatcttc acagcctcgg ccagttcgtt caagatggtt cgccgatttt
    10501 gtttgagaca attctctttg ccgataagcc caaaaacgat gtgctgattc cctctgatga
    10561 aagcgacctc gacggactta attatttggc gggcaaatcg ctcgcttacg tcaatgaaaa
    10621 agcattcttg gggacattag aggctcacgt gaaaactggg gaggttaata acatcctcct
    10681 gagcgtatca gagattacgc cctggaccct cggacacctc ttttatttct ttgagcgggc
    10741 ctgtgcgatg tcggcctatc ttttaggggt aaatcctttc gaccagcccg gggtggaaat
    10801 ctataaatcg aacatgttcc ggctcctcgg caagaaaggt tactaaggcg aataaacacc
    10861 actaagtggt gtttttttta tcgtttttac ctgatttgac aggagaaata tttattgact
    10921 ttacaatata tcaagtgata tattagttta gcacgcttat atggtgtcaa gtgtggatgt
    10981 ttagctcagt tgggagagca tcgcctttac acggcggagg tcgggggttc aagcccctca
    11041 acatccacca taatataatc gtcaggctcg tggaggaata gcgaagtggc caaacgcagc
    11101 tgactgtaaa tcagttccca cagggttcgg tggttcaaat ccgccttcct ccaccattaa
    11161 acttggggtg tagccaagtg gtaaggcaac agactttgac tctgtcatct cgctagttcg
    11221 atcctagcca ccccagccaa aaaaacttct cccgttagct cagttggtag agcacttgac
    11281 ttttaatcaa ggggtcggac gttcgaatcg tctacgggag accattctca aaatcatgcc
    11341 caggtggcga aacaggtaga cgcacaggac ttaaaatcct gcgggagatg atcccgtacc
    11401 ggttcaagtc cggtcccggg caccaaatta agccctttag ggctttttta tttgtgatga
    11461 attaaagtac ctcagaacaa gcacaagaaa caatgtagac aaattgtgtt cgttcattga
    11521 catctatttt ttagaaacga ggttgaaagc caagcactaa gattatataa tctttaaaga
    11581 atagttatat tttaaggagg acctcgatga acttgataaa aaaatatggc ccttacattt
    11641 cgatgggcct gattgtcatg gcacttggtt ttttatttct cccttatcta aggactggat
    11701 ataataacac gtattatgcc attggctacg aagccatttt taacattaaa gaaagttcta
    11761 taccttccac agtaagcaat aagggcggtc cgtcagtgat gctcatatta gcttttgtgc
    11821 tcatggttgt ttcgctcggc agtttgccgt ttcaccacaa ggaccctgtc atttcctttt
    11881 tagccggaat cgccctcagc atttcgggtg tcattttctt ccttggccag gttgtgatgt
    11941 ttattaatct acgcggatct gccatcagcg gcacattcgg tctttatctg gtggcaacgc
    12001 ttgtcactct cgccgccgcc atcagcctct tcgtgggcat ttcaaatctc agagcggtta
    12061 atcaaaagaa taacgataat cgctattcct atattcgcaa ataattaaaa aagattgccc
    12121 ttgacgataa tcttaaacat ggcaatcttt ttatttgaat aagatagttc gacgatcgct
    12181 tttgttctta tgtttataaa cgcgcttttt ggcgtattca ggaaggatca gcgtcgccat
    12241 ccctaagtcg gtgattttaa gaaacttgac atcgctattt tcttcaaata agtatgataa
    12301 atatcctcgt ctgactagat ttgtaaccag cgaagtaagc ttgcggccgc gatatgaaag
    12361 aagggtcgca aaggttgatg ttgctgaaaa tttgcgagtc tcgccatcgc ttattccatt
    12421 gaggatcttg gcgatgcctt cgacgcttgg aaaggcttgc tcccgattga gattggcgat
    12481 cgtttcaaga acttttaaat gggttatcga aatgcggaaa gaagaacttc ttttttccat
    12541 ataaaaaata ttatcacata acttgaaaag atgacctaat gtttctataa ttacggacgt
    12601 tataaaaata tggataaaga actgagaaac caaatcgagg ccaccctcaa caagatacga
    12661 ccattcattc aacgggatgg cggcgatgtt aaattcgtcg gttttgaaga tggcattatc
    12721 tacatcgaaa tggccggggc ctgcgccgat tgcgctttta tcgacaacac gatttccgat
    12781 ggcattgaga ttattctgat ggaagaagtg ccgggcatca tcgcggttaa acaagccttc
    12841 gagatgccgg aatcagcccg tcttgaatcc gctgaaaagt aaattactta attagcaaaa
    12901 gcccgcaaat cggcgggatt tttttcaatt agttggcttt ttgctcgtaa tttcgttata
    12961 aaagaacgtt ttcaatgcga ttggcgacga catagccttc gcggttataa acatcatcgc
    13021 ggttatagag gatgcgcgcg ccgctcggct tgacaaagac gcctccggcc tcctcgacga
    13081 caatctggct cgccgcggta tcccattcct ttgtcccagc cgacaaccga tatgtgatct
    13141 cggcaagacc gcgagcgatt cgacatggct tgatggcgct gccgaatgtc ttgcgatgtt
    13201 tgattttatc cttatgtctt tcgattgtcg caagttcctt ttcgttgaca tgaaagcgcg
    13261 acgtcagaac cgtcaacccc cgcgttttgc tattgacgcg aattatttta atgcgatttt
    13321 tcttctgata aaaactgcca ttgccttttg aagcatagta aatctcgccc gtggccggca
    13381 caaggacgac accgacgacg acctcatgct tataggctag ggcaatattg gtcgtaaact
    13441 catcgttttt atcaacaaag tcttttgttc cgtcaactgg atcgacaatc cacacataat
    13501 cattttgaag acgactttta tcgtcgcttg attcttcggt gagaaacgcg tgggtcggaa
    13561 aggcttgggc gataacctga cgaataattt cgtcggccat tttatcggcc agcgtcaccg
    13621 gcgaattatc ctctttgatt tcgacgtcaa aaggtttctt ataaatctcc attattttgt
    13681 cgcgagcctg aagagcggcg cgaatcgcga cacgaagttc attttcccac atagtcatta
    13741 ttctcctttg cgagcaaatc aagatctttg acgattgcca tcgcttcctg atagccgcct
    13801 ttttccattc tgactccggc cgcattggcg tgtccgccac caccgtattt tacggcgata
    13861 tcgcgaattg gaacgccgct gctgcggagt tcgacgcgca gcccgccttt ttcattttgg
    13921 gtgaaaaggg caaagatagg tgaaccctga atgttagcta gcacatttac ttgactggac
    13981 gcgtaagaaa attccaatcc gtaagcttga taatctttcg gctcggcata acaataaacc
    14041 actttattct ccgtcttttg ataatgcgac aaaagaaaac ccttgtattt caattggttc
    14101 tcctgcgttt gatagatgac atcatagatt ggcttcatct ccaaaccata atcaaaaaga
    14161 cggctgataa tatcaaatgt ttccttgcga gtcggagcat aaagaaagcg cccggaatcg
    14221 gtgacgatgc ccaaaaagat agcttcggcg gcccttttgc tgaggaggat gtttctttga
    14281 aaagcgatgc gggcgattaa ttcggccgtg gcaatcactc cgtgatccat aattttatcg
    14341 tagagaaagg gattgctttc gtgatggtga tcaatcttgc aataatcagg ggcgagtaaa
    14401 acgcgctggt cctcgactaa aggaaaatcg gaaacatcga cgagaatggc taaggattta
    14461 gcgattagtt catcgcttac tatgtccatt tcgccgaggc gcccaaaaag ggcggacagg
    14521 ccgcttccga tggcatagat ttttttatgc ggataggccg tttgaagaac ttcgcgtaag
    14581 gcgatctgac ttccgtagca atcgccatcg ggaagagagt ggccgaaaat aacgattgaa
    14641 tcgtattttt caatgaggga aaaaactgct gcttgcgctt cgttcattag ttcctccttt
    14701 tatcaaaaaa gcccctcgcg aggcttttta ttaagcactt attcggcttt gccgatacat
    14761 ccgaaaactt cgcatttggc gcgaacgact ttacgaattc ctttcatgcc ggggccgatg
    14821 attttccgcg ggtcataaac tttgctgtct aaagcgatga cttcgcgggt gattttggtc
    14881 cattcttgct gacattcggt attgacgttg attttgcttg tgccgcgttc gatagccatc
    14941 ttaatctgga aatcaggaat gcccgaaccg ccgtgcaaaa ctaagggagt tgtgacgagt
    15001 aagtcgcgaa tttcagccat ctctttaaaa ccgagtttcg gctcgccttt gtatggtccg
    15061 tggaccgagc caagggccgg agcgagacaa tcgatacccg attctttgac catgcgcacg
    15121 cattcgaagg gatgggcata catggtctcg gccacgacat cgtcttcctg accgccgacg
    15181 cggccgagtt cggcttcgac cgaaacaccg cgggcgtgag cgtaatcgac gacctttttg
    15241 gtcatctcga tgttttcttc aagcggatga tgcgatgcat ctatcatcac tgaagtaaaa
    15301 ccggcatcaa tcgctttgat gcacatctca tatgaacttc cgtggtcgag atgaagggcg
    15361 acggggacgg tgatgttgcg gtctttgata tagcctttga ccatgccgac aatcgtgtta
    15421 aatccgccca tgtatttggc ggcgccttcg ctgacgccga ggatgaccgg ggctttgagc
    15481 tcctgggcct cttcgaggac agctgatgtc cattcgacgt tattaatatt gaactgaccg
    15541 acggcgtact tctcttttaa tgcctttaaa agcatttctt tcatactgac taatgccata
    15601 attttttctc cttatttaga ttttaataca aatcttcgat ttttactttt tcttcgtctt
    15661 cgacttcttc aacttcttcc tcgagttcat ccttatcatc gtctttttcg attttcagat
    15721 actcgatttc ttcctcgtcg atttcttcgg tttcttcaac ttctgaatag acatcattca
    15781 tgtcgatgtg gactttgtca aaagtgtggt gtgagcgaag atcccaaaaa ttatcagtca
    15841 gggccacaaa gcgaccatca agtgagatgt ttgtataaaa acggctgatt tgggcgcgca
    15901 tctcctcttt agtcagttcg gctttgatgc agacggcttc ccacaactca tggaaactaa
    15961 tcggcaattc tttagcttgg gatatgacct cataggcgag gtcgagcatt gatttttcgg
    16021 acatgtaatt ctccttgtta catttgttcg ggggcagtga cgccgaggac gttaagagcg
    16081 tttctcataa cgatttgact agccttgcaa agggctaaac gcgattgcga caactctgga
    16141 ttgtcgcgat ctatcacacg acattcggtg taaaaagcgt gtgttaagcc cgctaatttc
    16201 tgaatgtacg ttgcaacctt atacggctcg cgagttttag cgcagctttc gatggtaatc
    16261 ggaaactctt gaagatgctt taacagattg atctcggaag actcgcgaag attcttcgcc
    16321 gagatgtcaa ggccgatatc ggctcccgtt tcaaggagct tacacagtcg cgcatgggcg
    16381 tattgagcat aataaacagg attcgaggaa ctgcgttcta acgctaaatc ataatcgaag
    16441 tcaagatggc tggtcgcggc gcgcgcaaca aagaaatagc gcgtcgcatc gactccgact
    16501 tcttcggata attcgcgaag agtgatggtc gtgccgcctc tttttgacat tttaacttcg
    16561 acatcgccct tcatcagacg gacgacttgt acaagctcaa aatcgataat ttcggcagga
    16621 taccctttca tcatcagggc gcttcgcatg cggtttgtgt agccgtggtg atcggctcct
    16681 aaaacatcga cgagcaaatc atacccgcgc gaaagttttt caagatgata ggtgatgtcc
    16741 ggcatcaaat aagtgtaaga cccgtcactt ttgacgacgg ggcggtcttt gtcatcgaga
    16801 aaggctgatg tccttaagaa agtcgcgccg ttatcaagat aggtgaattc gcgatagtct
    16861 tgtaatgttt tctcgacctt gccgtctttt ctgacatcgg tctcaaaact gtaaagatcg
    16921 aaagtcacgc gaaaatcagc gagatccttt ttaatttttt caagttcgag gcgaatgcct
    16981 tcttgtatga aagtcttcat gttggcttcg gtgtcggcca cgagtttgtc accgtggatt
    17041 tcttttatca gtttagcgat gtcgaccaaa tcaaggccgt gataaccatc ttctgggacg
    17101 ctggcatcaa tgccgaattc attgagataa cgggctctga gcgaagcccc catcttattt
    17161 acctgggcgc cggcatcgtt gacgtaaaac tctcgggtga tctcatatcc ggcaaattca
    17221 taaaggcggc agatcgaatc gccgatcgcc gcgcagcggg catggccgag atggagatct
    17281 ccggtcgggt tggcgctgac aaattcgaca ttaatctttc gtttttttgg cattccccga
    17341 ccgtaattac tatcctggga aataatatct tcaacgattg cctgcaaggt attcgaggcg
    17401 atgaaaaaat ttaaaaaccc cggtccggca atttcaattt ttactaaacc gctgcaatcg
    17461 atttcctcga caattttcgc ggccaaatcg cgtggtttga ggccgattct tttggcgtgt
    17521 tgcaaggcgg cattagtcgc gtagtcacct ctcgtctcat ctttagggtg ctccaaaacg
    17581 acttcaccct cttgaatatc aaggccgatt tttttaatgg catgtaagat tttattcttt
    17641 aagaaagttt cgatgttcat atgtttttgt caaagtaata ttatacttat acattagtat
    17701 aattcaatct ttatcttggc tgaggcgtct tagcgtttcc gctttgccga gaaatttaat
    17761 aatttcttcg agctccggcc ctttctccat gcgtgttaaa aggaggcgca ggggctgata
    17821 aaaagctttg ccggcgatgc cggtcttctt ttgcgtttcg tcgaggaggc ttgcgacatt
    17881 ggttaaatca tcatcgcaat ttgcgagtct ttcataaaat gaagacagca cactctgcgc
    17941 ctgcggattt gagatgattt gctgttcgcg gggcgtaaaa tcaggatctt gcagcaaaaa
    18001 aggtgcgatg aattcttcga tttcgcggcc gtagctgatt tggttttgaa agaccaatgc
    18061 cagttttaac aaaaaatcat cgctatattc ttcgctgata aaaggttgaa taaaagggcg
    18121 cataaaagcg gtatagtcag atgttttcat ggttttaata aaaacggagt taatttttct
    18181 aaggattttt tcatcaaaaa gctgatggtc agtccgtata ttgtcaattt cgaaagaatt
    18241 gacgatttgc tccatattca tccgataatc ggccttatta tcatcgacat caagacgata
    18301 taagtagctg caaatagctt ctggaagata accgtttttc ttaaggaggt tcaggatttt
    18361 ttcgtcaaag tcgcgatttg ttttgccgat cgatatatga ataaaagttg ggagctgcca
    18421 attcaaggcg cgatttaatg cgacatagcg accgagattg gggagattag tcaactcaaa
    18481 aacgacatgg gaaattccca tcaaatggtc atcgattatc gccgcgaatt cttcgctggg
    18541 caatccgtta ctgccggcga ttatccaatc gtttaaattc agcttgtcat cggtgacctt
    18601 tccgatgcgc aggtcgttga ttttagtttc gccaagagcc ctcattttga agtaggtggc
    18661 cggacgaaaa gcaccgagag gagagccttt ttctttgggt ggaactcttt tgtatgccgc
    18721 tcccacggcg agcagcgcat cgatgtatct ctgataaata tcgaggcgtt cattttgaac
    18781 atatggagaa aagccgggat tcggatttaa gggtgactcg tcggcgatta agccaagcca
    18841 gtccaaatcc tcgagagcaa aaagattggt ggtcatacca tcttcttctg atcgcatgac
    18901 gttatcgagg cgaaagataa aagagcccct cagttttcgg gatattaaat aagtgagaaa
    18961 tgcttttctc aactgactta aattgagatg cccaccatct tgatgagcaa acctcaagcg
    19021 cgtcatcaca aactatttcc tctgcagcat gacgacggca acggcccggg cggcttcgcc
    19081 acgccccacc gcatctaggc cttcattcgt catggcttta atcgagacat ttcgatccat
    19141 aattcgcaga gagcgcgcaa gattggcgac catcttcgag agataaggtg aaaggcgagg
    19201 cttttcaagg gcgattgaaa tgtcaatatt tttcacggca taaccgtttt cttcgataag
    19261 ccgattcact ttattgagga gctccatgct atcaacattc ttatactgct tatcggtgtc
    19321 gggaaaatgc gtgccgatgt caccgaggga gagggcgcct aaaagcgcat ccatcagagc
    19381 gtggatgaca atgtcggcat cgctatgtcc aagcagtcct ttttcaaaag gtatttcaac
    19441 tccgcctaaa atcaatttcc gacccgcgac gaggcggtga atatcttcac cataaccaat
    19501 tcgatacata gtataacctc ctaaacatta aatctaaaaa acatgatatc gccatctttg
    19561 acgagatagt cgcgaccttc gatgcgaagt tttccggctt ctttgaccgc caattcgctt
    19621 cctaaatgga aaagatgttc ataactatat acttcggctt tgataaaacc tttttgaaaa
    19681 tcggtatgaa tgatgccggc gcactgcgat gcactgctgc cgtttttata cgtccaggcg
    19741 cggcactcat cggcaccgac ggtaaaaaag gttgccaaat caagaaggcg ataagtggcg
    19801 cgaatgactt tatccaggcc ggattcgcta gctcccatct cctttaagaa ctcacgcctg
    19861 tcttctttgc taaggagcga aagctgatat tcgatttcac acgaaatcgg aatgacttga
    19921 gctttttcct gaccggcgat gcggacgact tcctgataat gtttggcgat actcaagtca
    19981 gcataatctt cttcgctgac attggcgaca tagatcagcg gtttcaaagt tagcaggtga
    20041 aagtttttgt tagcatagct aatttgctcg ctgctcaatg gggcattgcg cgccgaaatg
    20101 ccacgattaa ggttttcttt tataatcgcc agaatcgcca tttcatagac ggcgtctttt
    20161 tcttttgcca ggcgagcttt gttttcaact ttggcaatgc gcttgtcaac agtatctaaa
    20221 tcggcgagcg ccaattcgaa gtttatcgtt tcgatatcac gttggggatc aatgctgttt
    20281 tcgacgtgga taatctcgtt cgattcaaag cagcggacga cctgaacgat ggcgtcacac
    20341 tcacggatgt gggcgaggaa ttgattgccg agtccctctc ccttggaggc cccgcgaacc
    20401 aaaccggcga tatcataaaa ttcaaaagtc gcatgaatcg tcttcagtgg tttaaagaga
    20461 ttggatagaa aatcgagcct ctcatcggga acgaccacta caccagtatt tggtttgatg
    20521 gttgcaaagg gataattggc ggcttcgacc tgcgaattgg taatcgcatt aaaaagtgtt
    20581 gatttaccga cgttgggaag accgacgata ccggctttta aaggcatgga gaatcacccg
    20641 cattcataaa taagcattgt tattatataa caagcgtcat gagatagtaa gaagagaaag
    20701 aaaaagccct aatttattag ggcttacatg ttcaatgact taatttttaa cttaaatctt
    20761 cttgatgttc gcggctctta agccacgagc agattcctca acatcgaatt ctactttttc
    20821 gccgacttcg gcggtcttgt agtcctccat gactaaggat gaataatgga aaaagacgtc
    20881 tgagccttca tcggtgtgga tgaagccgta acctttttcc ttattgaaca ttttgactgt
    20941 tcccttcatg aaatcctaac ctttcttaca atagtatttg tgagtcattt aactcaccgc
    21001 catataatag cattcaaata agaatttatc tattattttt tgcaaaattc tttactttaa
    21061 taaattgctt tttaagcgat gaatactgtt tttcgaaggc ggcatagcta gcggcgacgg
    21121 cgatgacaac cgacatagcc aagatcgccg ccagcgggcg atagtcgaag acataaggga
    21181 tgttcgtgcc cacaaaaccg gcgatgacgt gagtcacgaa ataatcgaga atgatgattt
    21241 ccagacccga taagaaaaat gataaagaag acacaaccag tccgcagacg atgaaagatc
    21301 ttcgaatcgc cgatttcgaa attccaagat agtcgaggag aatgatgtcg cggcgatttt
    21361 cttcaatcgt gacgaacatg acgatgaaaa agagaaagag agaggttaaa accgcgagtg
    21421 aggaaaaagc aaaaagaacg actctgatga aggacatcgt ctcgtctagc ccatcgctta
    21481 tcgtcttcat cggatcatag aaatcgaact ctccatattg cttattaagc ttttcgacta
    21541 aaagctcgct atcgccgttc ttgtcaatct cgaaaataac gtgtttagga attaattcga
    21601 aagcgctgac tcctcctttt aattgaaaga agcccaggct ccaatacggc cgatgatgaa
    21661 gggtgatgcc gctcccgcgg ataatgccga caatcgttgt ttccgtcaat gaatactgtt
    21721 tctcgatgcg gtcattatca taggcgatat tggcataatt agtggcgatt agcagctttt
    21781 tgccgaccat ttcgacaggc gaaaggaact tatgtgctaa agtctccgat atcgcaattt
    21841 cgtcaagatt aatgggcttg cggccataaa ttacttctct taaatcgctg gaaaatgaca
    21901 ctcctccatc catgctcgtt tgaatgtttc cggagaaaac cccgggaggc ggctcaaaga
    21961 tcatattgtc attttccgag gcgatcagac tatcggcgtc gatggctttt tctatctgcg
    22021 tatcgctcag cgagatgaag atgtttttgg caaagcccgc cagaaaggaa tttggatgat
    22081 aataatatcc gccatccgtc gcatacaagt ggttttttat ctgatgctct tttttcatca
    22141 ttccatcaag agctgaaata tcaatcgtgt ctttatcgac atgaaagacg aggaggcgtt
    22201 ttaatagaca cgggccgctc atcgggcaat gggtcggatg gaaggaaaag cccgccattt
    22261 caaaaacaaa atcgcgcagt ggcttctcga gtgtggccac ttcaagaaat gtttccggat
    22321 tctctttcgt cgtcacaaaa tagcttttgg ccatctgcca cggaaattca tatttgccat
    22381 catcaatgga aggaatgcgc attttttcct gaaagacata ctcgctccaa aggtgatttg
    22441 tatgatagat ggcaggcgca tcgcttggaa caactccact taagtggaaa acttgctcgt
    22501 ctccatatcc ccagtcgcca ttggcgattc ctaaagtaac cagggggcgg acaccgctga
    22561 tgtattcgcc aagagacgca taatttcttt caattctcag cgaatagcaa acgccgacca
    22621 tttcgttatg cggaaggccg ataacaatct cgtcattgtc catgcttgcc tgctgaaaag
    22681 gataaatttg gtgatttgat ccaatttcat caagccataa aaaatcgtta atttgacgcg
    22741 ttgaaaagcg cggcagaatt atcttataag cagttgaaga gataaacatt tcgtcgcgat
    22801 cttgaaaaaa agattcgaaa ttgacggcat atgagacacc gataccatta atccatgatc
    22861 cgtaagtctc agctatttga ctgacgtttt caaagctcgc gctgtaggcg gtaatcggtg
    22921 tctcatcacc tttttttgac atgacaatca tgccatcttt gatgacttgc gaaaagccgg
    22981 tgacgattcg ctcctgaatc gaaaatgaca ttattatcga aaggccgatt cccagaagac
    23041 caagagacat catgctgttg ttgagtatga cgcgatattt gcgcgttttg ataatcgaaa
    23101 gcgctcttct aagcatcgtt ttaaaaggca tatgcagttt gttgttggct tttttcatct
    23161 gctgaaaagt tattttcttt ttgatggcgg tacgttcaaa tttttgatgt ccggtgatca
    23221 ttccatcgac aattgtgact ttgtcatcgc agtagcgagt cacgagttct tcatcgtgag
    23281 agacgacgat cacgagccgc tgggctgaaa ttgatttaag aatgtccatg actttgttgg
    23341 cattgccttc gtcgagcgaa ccggtcggtt catcgcagag gatgaaggcc ggattattga
    23401 ccattgcccg cgctatcgcc acccgctgtt tttcgccgcc ggagagcaag ttcaccgcca
    23461 catctttttt gtggcttaaa ccgacgaggt cgagaagcga cgatacgtgc cgttttttaa
    23521 actccgcttc atcatttgac agcacatcga gaggaagcag taaattattc atgaccgaat
    23581 cgttttcaaa gaggcgaaaa tcctgaaaga cataaccgac attgagcagt cggaatgcgc
    23641 ttttctgctc tcgggtcaga taatcgaccg acttgttttt gaacagataa tcgccctgat
    23701 acttttcatc gattccggag aggatattta gcaaggttgt cttgccgcat cccgatggac
    23761 cgacgattgc gagaaatccc gtcttgggaa agcgaagagt tatgcggttg agtactcttc
    23821 ttgttccgta ttctttgatg atgtgattta agcgaatcat cgtcagtttt ctctaagctc
    23881 gtccgcaagg gataaacctt tataaaggcg cagtggcaaa tacgtaaaaa caatcgtgat
    23941 gacatagccg gaggacaaca cgatgaatat caggaaaaag ggtcgaccca taaaagcggc
    24001 taatggaata tcgataagat tcttaaacat aaaatgttta tagaagtact catttgctat
    24061 tttctgagca aaaatcgcaa aggcaaaagc agcaattatg gcagcgaaaa tgatgaataa
    24121 actcttataa agaaagatgt cgagaatatc atttttccgc gctcctatcg aagttaaaat
    24181 cgctgattcc ttctttttcg aaacataatt ggagtaggcg cctatcgcca tgattgaaca
    24241 tgttccgacg agggcgataa caagaaagac gagcattgaa taaaaggcgg cttttgtcag
    24301 ttcctgatat gtatttttta tgagcagtga tgacgattcc aacgccaaat tggaaccgct
    24361 gctctcgagt ttttttgaaa tctcggaaac gacaggtaaa tcgtctttgg atagcacgaa
    24421 cacgtttaaa tcgtaattgg tgagatagtg attgtcggcg atgatgcgaa agatgtccag
    24481 acacgaaatg tctctgttaa agtggtcgct tatatcttct aaatagtaat tttcgagcag
    24541 ttcctcaaag gcgacatgag aatagaatat tttgggagtg ttaagaaagg atagttcttc
    24601 aaaaattccg cttatcatca tttccttctg ccacaggaaa acatcttcga taacgctgtt
    24661 gtattcatca tcgattgtta tcttcgtctt gatatagatt gttaattttt gtccgaccaa
    24721 attgtgaaaa tcagttgata agattgtggc aaacgtctta tttatcaaca cttcttccat
    24781 cgtctcttga ttgggaatca tgccttcgat caggagctcg cttttctggc gcagatgcaa
    24841 atagctatag actggcgaaa aaaccggatc gtcgattctt ttgtcgtgaa aaaacaaatc
    24901 gtggttaccg ggaaacactt tcgtgaagtc gtcggtcacg atcgccgagc tgacaatacc
    24961 gctaagaaag ctcaggtcat cgcgtcttgg cctagttact ttgatgagcg aaagcggagc
    25021 gtcaccactg cttatccgct cctttttcgt cgctttaaaa agagcgctat cgacgatgcg
    25081 ctcctgtgtc gtatccacga cttgattcgc cccgtaataa aacccgacag aaagcaataa
    25141 cgaagcaaaa ctaattaacg tagaaaggaa caatataata tttttgctgg catcgcccca
    25201 aatatacgag cgagctatct tctctttcca attcgaaata atcacgattg ttcaggaaag
    25261 gagacatcgg agacaatctg gccgtctttt acttcaatgt gccgcgtcgc ataggctaaa
    25321 gccaaatcga gattatgcga gacgacgatg acgaggcgct cttttgatat cgctttaagc
    25381 atctccaacg tctttatggc gttttcccga tcgagtgcgc cggtcggctc atcggccaag
    25441 acgatggcgg gatcattaat aatggccctt aaaatggcga cgcgttgctt ctcgccaccc
    25501 gagagttttc cgacttccac atcatgcatt gaatcgagct tatatgcacc cagaagtttt
    25561 cttcccgtct catacgattt actgattccg cgcccatcga ttagcagggg taaaacaaca
    25621 ttgaagagca cgctctgttt atcaaataaa ttgtactgtt gaaaaacgaa accgatttcg
    25681 cgattgcgat aagcgcagat gtgcttttca ttcatcccat caatcgtttt gttcttgtag
    25741 agcacgtgtc cgtgcgaagg cttcaaaatg ccggcgataa gatttaaaag ggtggacttg
    25801 cccgatcctg atttcccgga caaaaggaca aagccccttt cttcaaaggc taaatcaatg
    25861 ttttgcaaaa caggatagtc ctgccctcca agcgaaaaga cgcggcttaa gttgtcgatt
    25921 tttaaaattg gtgtttccat agttaccaat atataagggt agaaaacgcc aaaagtatca
    25981 gttatttttt atgacaatcg ccttttcttc gtgagcgatg actttgcgaa ggcgcttatt
    26041 gaagtcatag cgcgtcagca taatgacatt tccacaaccc agacacatca tcttgatatc
    26101 ggcgccgacc cgaacgatct ggaatcgttt cgtccgtgcc gagcaaggat gttctttctt
    26161 catttcaaca atgtcgaata attggtaatc gagcactttc atattaatat ggtaaatctt
    26221 ccattaagac cgccgacgga acttttggaa gtcccggcat cgtcaaaatg tttccggtca
    26281 gagcaacgac aaagcccgcc ccggccgata aacccaattc acggatggtg attgtgtatc
    26341 cttcgggcgc gccgatgact tcaggattgt cgcttagcga aagcggtgtt tttgccatac
    26401 agacaggcag agcgccatag cccagctgat tgaattttgc aatctgctcg cgtgcaaact
    26461 tcgaatattc gacttttccc gcgcggtaaa tctgtttaca aatcttttct attttctctt
    26521 caatcgataa atctaattta tataagggct tataatgcga aggctcggta tcgagcatct
    26581 tgacgacttt ttgcgctaag tcgacggcgc cttcgccacc ttttaaataa ccgtcgagga
    26641 aactgacgac atagcctttt tcttcacacc atttctccag agcttcgatt tcgctttgat
    26701 gatcgctggc aaagtggttg atggccacga cggacggaac gccgaatttt ttaatgtttt
    26761 caagatgaac gcgaagatta ttgacgcctt taagcaaagt ttcgacgttt tcttcactga
    26821 ttttttcaag gagctggccg ccatgcattt ttaaagcccg caaggtggcg accatcacga
    26881 cgccatccgg tttcatctcg ccgtagcgac aggcgatgtc aaagaacttc tcggctccca
    26941 gatcggagcc aaaaccagct tcggtaatag cgattggggc taatttgcgg gatagtttta
    27001 aagcgattaa agagttgcag ccatgagcaa tattggcaaa cggtccgcca tgtatcaaaa
    27061 ccggattgtt ttcaatcgtc tggacgagat ttggtttaag cgcatctttc atcagcttca
    27121 tgatcgcgtg cgaaatcttt aaatcccgca ctcggatcgg ttcttcgtca acattaaaag
    27181 cgacgatgat tttctgaacg cgttcgcgaa aatcctcttc atccttagca aggcaaagaa
    27241 tggccatcaa ttccgaagcg acggtaatca caaaaccgtc ctggcgctca atgccatttt
    27301 tttcaccgcg gccgatgatg atgtcgcgca aagcgcgatc attcatatcc atcgcccgtc
    27361 tccagacgat gcgctgagga ttaatcctta actcgtttcc ctgataaaga tggttatcga
    27421 tgacggcggc gataagattg atgctcgaag taagggcgtg catatcaccc gtaaaatgga
    27481 ggttgatatc atcgcgggga tcgatggaag caagtccgcc cccggtcgct ccgcctttga
    27541 tgccaaagac aggaccgagt gagggttctc ttaaagagag tatggctttt tgattgatct
    27601 tcgccattcc ttccgccaaa gcgatggatg ttgtcgtctt gccttcgccg gccttcgtcg
    27661 gcgtgatggc ggtgacgagg acgagtttgc catcgggaac atcctttaag acgtcaaaaa
    27721 tactagtgtc gatcttcgct ttaaatttgc cataaggttc gatataggta agcggtatgc
    27781 cggcaatctt ggcaatttca tcaatttgta aaaggggcat tttcttacct ccgttttctt
    27841 atatatagaa tataacttaa taaaccttac ttttgtatct tttctaagca gtaagatgat
    27901 agggcgagac ctgccgcaga aggaacattc atcaccgagg cgagcaacga acccttgcga
    27961 aggggctttt caagagaata gacgaccgga atatccgcta aatcaatctg ctctctgcgc
    28021 atcagcatcc gcattttctt ggcgagggga tcagtatcgg tttttgctag attagcgatg
    28081 cgaaccgcgg tgggatcaag gcggttggcc atacccatcg atgaaataaa aagcagattg
    28141 ttcgccaagc agtgtttaat caattggact ttcgcttcgg gggcatcgat tgcatcgatg
    28201 acaaaatcga atttgcggcc gctgattcga gtcaaaaaat tatcatccac tcggcagtca
    28261 aatgccgtga caataatgga ttcgtcgatt gtttgaacac gttctttagc cacttcaact
    28321 tttggcttac caacatcctt atttgtgaaa agaatctgcc gatttaaatt tgatatctcg
    28381 acgacatcgt aatcgacgat ggtaaaatgg ccgatgccag agcgagcgag gctttcgagc
    28441 gccgtgccac cgacgccgcc cagaccgcag acgaggacac ttttatccct taaagcctta
    28501 actttatctt cgccgaccag ggcggcaaat cgttcaagac gtttttccat ggctgatctc
    28561 gataatgtca tcgacacttt ttatccaatg cattttgaac tggtgacgcg caaacgtcat
    28621 ctgtcgcttt acataatgac gggtattcgt aatgatatca ttgattgact gctctcgact
    28681 cgttttacct aaaaggtaac tgatgggttc cttatatccg atggcgtgaa aagcttgctg
    28741 tttaactccg tattcattga ttaaattgcg gacttcttca aagagtccgt tttcaaccat
    28801 gcggctgacg cgctcgacaa tgcgaagata caaatcggca cgttcaaact caatcccgaa
    28861 aaagagcgtt tcatagagtg gctcagaatc ctttttaaga ggaatttcag atttttttat
    28921 gccttgcgac cgataaattt gtagagccct taaaacgcgg cgccgattat tgaagtgaat
    28981 cgtttcggcg gctttggcat caatgacttt taaggcgtta tggagcgcct gattatctaa
    29041 tgaatcatat tgtgataaat cgacctccgc ctcttcttta aatcgataat caaaaagggc
    29101 ggctttgatg taaaggccgc ttccaccaag cataatcacc ggtatatttt tcgataagag
    29161 atcgtcgatg atcacccgcg cttctttttg atagcgggca atagaataat tctcgtcgac
    29221 atcaacgatc gcaaaaaggt gatggcgaac gccttggaaa tcttcttctt taggcttgtt
    29281 ggtgccgatg tgaagctggc ggtaaacatg aaaagcatcg gcgttgatga tctccccgcc
    29341 caggcgcttg gcaagttcta tggcaatccg gtctttgccg gtattagtcg gaccggtaat
    29401 aacgtaaatc ataatttaat tatagggcag aacgatgaaa aaacatcgct aatcaagcaa
    29461 atcggcgata tttttcagaa aattgtgcac ttcagactgg atgcttgtga agttggccac
    29521 aagcggatga ttatcataaa cagctaacaa attctcatat tcagccttct taagagcgtg
    29581 ttcttgttca tcggccatac tcaatgacag ggcgacttgg gcggcattga ctttaatttt
    29641 gagcgctttc agttcttcat tttcgccaat taatgcgcgg aggcgaaaat attctttaat
    29701 aagttcgcta ttcatgattt cacgttcgag attatcaagg gcgttttcaa tattattcat
    29761 cgacgagttc tccaattaat gaataggtgt gggattcaag gatgcggacg cggacaattt
    29821 taccgatgtg cgaggcctcg ccgcgaaaat ggacgagttt gtttgtttca ctgtagccgg
    29881 agagtacaga tttgtctttt ttactccagc catcgacgag aatttcgtaa atattaccga
    29941 ccatgccata tgacgattct gcaatcgtct cttcgagagc ttttgtcaga attttaaagc
    30001 gcgccgcctt ttcttttttg ctcacgttgt caatgagttt ggcggctggc gtcccttttc
    30061 ggggtgagta gataaaagta aacgcgctct cgtatttcac ttctttaata agtgagacgg
    30121 tttccataaa ttgttcttcg gattcattag gaaaaccgac gatgatatcg gtgctgaggg
    30181 caatgcctgg aagttgatcg cgaagttttt ggacaatttc caaatattgt tcacgggtgt
    30241 atcttcgccc cattgagcga agaatctcgt tgctcccgct ttggacgggg agatgcacac
    30301 atttcattaa gttctcatgc cttttcatca cttcaatgat atcgtcgcta aaatcaaaag
    30361 gatgcgaggt caaaaagcgc aggcgcgaaa tgcctaaaag cgcaactgcc tcgagaagag
    30421 tcgcaaaggt gacattacga tttttaaagt ctttcccata agaattgaca ttttgaccga
    30481 gcaaggtgat ttcttgatag cctcgttcga cgagatgacg acattcggca acaatctcat
    30541 caaaggggcg gcttcgctcc tgaccgcgcg tgtaaggcac gatgcaatat gtgcaaaatt
    30601 tatcgcaacc ataggagatg ttgacaaatg ccttgtgttg cgccttgcgt gtcgatggca
    30661 gcttttcaac gatttcctgc ggtcgggcca aaacatcgac aacgcggcaa gactcgcgga
    30721 gatgaatgtc gattaactca attattttat gaatgttgtg cgtgccgaga ataaggtcga
    30781 cctgcggata cttttcaagg agcaagtcaa tgatgtgttc ctgctggctc atacagcccg
    30841 agacggcgat aataaggtcc ttgtttgcgc tttttaaagc tttaagcgtg ccgagttcac
    30901 cgaggacttt gtcttcggca ttctcgcgaa ccgcgcatgt gttgagcatg ataaaatccg
    30961 cggactttgc gcttgctgct ctgacaaatc cggctctttc aaggattgcg gaaataactt
    31021 cctcatcgcg gatgttggct tgacagccgt aggtgcgaat ataatagcgt ttgttttttg
    31081 cgaaatctcg aactaaatca ctgacaaacg gttcataaag atgaactggc atcaatttct
    31141 ggcgaattct taattcgtcg agatcgggct tcgaaagaat cttgggttta gccatcgtta
    31201 cgaccgcttt cgacaatata tatcgggaaa atttccttgg cctggcgcgt aaaattatca
    31261 atgtcgagga caaccgccga cagaagtgac tgatcgcctt cgtcaagttg gaaaatgtca
    31321 tctttaccaa aaagtgtgcg attgattact tcttcttttt taaaaccaat gacaccgtta
    31381 taagctccac acatgccgac atcacttata aaagccgtct tgttgggaag gagtcggtaa
    31441 tctcgcgtct ggacatgcgt gtgcgttccg ataatggcgc tgacttgtcc gtcaaaagca
    31501 aaggcaaagg cgattttttc agccgttgct tcgccatgaa aatcaacgat gtgaatttca
    31561 tcttcaggcg tttcggcgag gagatccttt aaagcgtaat atggatggtt tacttcttcc
    31621 ttcatgaaag ccgtacccaa gagattggtg acgcgaatct tgacgccttt gacgtcaaaa
    31681 acgatgcttc ctgaccctcc cattgatttt aaaatattca ggggtcgaat caggcactca
    31741 gcctcgtcaa tgtagcgacg aatatcgctt ttagtcgcgt agtgattacc taaggtaatc
    31801 gcatcgattc cggcatcgat cagttcatga taatgcttct cgattaagcc tttgccgtga
    31861 gtggcgtttt cgccgttggc aatgacaaaa tcaatccgat acttatcgat gagttggccg
    31921 agatgtcgtt tgacgacttg ccggccgttg cgaccgacaa tgtcgccgaa aaagagaatt
    31981 ctcatgagtt taggtgcttt ctgttccgcc tttattttgc ggtttcaaca gcccgatatt
    32041 cacggatgac cgaaacttta atctgacccg gataggtgag ctcgttttcg attttctcgc
    32101 gtatgtcgcg agcgagttta taagcggcta gatcatcaat tttgtcgggg atgaccatga
    32161 cgcggacttc gcggccggac tgcatggcgt atgattgata aacaccatcg tatgatttgc
    32221 aaagactttc gagtttttca atgcgcttaa tgtaattttc gaccgtttcg ctgcgcgcgc
    32281 cgggacgggc ggctgataaa gtgtcggcgg cggccacgag gtgggagatg acgaattttg
    32341 gttcgacgtc gccatggtgg ctttcaatcg aattgatgac gacttctggt tcgccgtact
    32401 ttttggcgag gcgaactccg atttcgacgt gggaaccatc gatttcaaag tccatcgatt
    32461 taccgatatc gtgaagtaac ccggcgcgtt tcgccaggtt ttgatcaagg cccagttcaa
    32521 ccgccatgat gcctgctaaa taggcgactt ccatggcgtg gtcgtaagcg ttctgaccgt
    32581 aactggtgcg gtatttcagt cttccgacat aagttagtaa ttctttatta atgcgcggca
    32641 gtccaagctt gaaggcggca tcttcgccgg ctttgcgaat tgactcatcg acttcgactt
    32701 taactttttc aacgacttcc tcgatgcgcc ccggctgaat gcgaccatct ttaatgagtc
    32761 gttcgagcgt caacttggcg atttcgcggc gaatcggatc aaagcatgaa acggtgatga
    32821 cttcaggcgt gtcgtcgatg atgaggtcga caccgaggag ctgctcgagc gagcgaatat
    32881 tgcgtccttc gcggccgatg atgcggcctt tcatttcatc gctgggaagg gagacggctg
    32941 agacggttct ctccgtcgtg acttcttgcg cgtatttgtt gatggcaagg ccgattaact
    33001 cgcgagcttt ctcctgcgcg gtttctttgg cttcgtcttc tttattttta ataaaagcgg
    33061 aaatttcttt tgataatttg ctttcaacgc gctggaaaat ttcttcgcgc gcttcattga
    33121 ttgaaagctg ggcaactttt tctaattcct gtatgatcga atcaattttc tgctgaagga
    33181 cttcttcctt gcgctcagcc tctttaaggc ggcgattcaa tgtttcggtt ttttcttcca
    33241 ggacgttttc cttggtgatt aactgcgcat cgcgacggtc gatattagct tcgcgctgcg
    33301 agagtttgtt gtctaagacg agatactcgg cctttctctc tttgagttct ttttcggttt
    33361 cgagtttcat ttcgtaggcg acttgtttgg cgtcgatctg agcagacttg acggtgtgct
    33421 cggctttaat ttcggcgtcc ctgatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33541 nnnnngaagt cacaattgat tacctcgtcg gtcttgaaga aaaaactctt gtcgaacaga
    33601 ttcgcgatcg aattcacgag ctgaatcgag aagagttagc ggatttactt ggcgattata
    33661 ttgaagtaat cgcaaaaatc aaatagatat tattgggcaa ttccggtttt tagcttagtg
    33721 acgagatcgt gcatcacggt ctcgtttttc tttaaaaatt ccttggcggt ctcgcggccc
    33781 tgaccgattc tttcgccgct atattcatac catgatcccg atttcttaat cagtcccata
    33841 tcgatggcaa ggtcgagaac ttcgccctct ttagagatgc ctttgccata gatgatatca
    33901 actttgcagg atttaaaagg tggtgcaact ttgttcttga caactttaat attgaccgta
    33961 tttccgacga gatcgttgcc atctttaatc gcttctgaac gacgaatgtc gatgcgaact
    34021 gaggcataga atttaagggc tcgtccaccg cttgtcgtct ccggatttcc atagacgatg
    34081 ccgactttct cacggagttg attgatgaag ataatcgtgc atgaagagcg gttgaggacg
    34141 gcgacgagct tgcgcatcgc cttcgacatc aagcgggctt gaagtccgac ctgattatcg
    34201 cccatcaaac catcgagttc cgcttgcgga acaagagcgg cgacgctgtc gacgacaatg
    34261 atgtcaatgg ctccgctcga agctagcatt tccacaattt ccagtgcctg ttcaccacta
    34321 tcgggctgcg aaagaattaa atcatcaatg tcgacgccta aattgcgggc atagatcggg
    34381 tcgattgaat gttcggcatc gacaaaggcg gcccgaccgc ccatcttctg agcttcggca
    34441 atcgcgtgaa gcgccagagt tgttttgccg ctgctttccg gtccatagat ctcgatgatg
    34501 cggcctttgg gatatccgcc gacaccgagt gaatagtcca ggagaagcga acctgaagga
    34561 atcgcgtcaa cttcgacgtg tggtctttcg ccaaggcgca tgaccgcgcc tttaccatat
    34621 gttttttcga tggcctttaa cgcctcatcg agggcggaca ttttgtcttt agatgtttct
    34681 tttgccattt tttatcctca ctagatatat aggaacgaaa acatgattcg ttgctaaatt
    34741 attttaaaat agtttccaga gtgctgatca tcagcgcggt tgcctgcttt ttaatttcgg
    34801 tgcgagaacc gcttaaattg agggggacac cccatatatt atctttgctg gctactgcca
    34861 aaaagacgga cccagcttct ttttttgaat cgctggcggt cggtccggca ttgccggtta
    34921 cggcgatgca gtagtcgaca ttcaagagcg tgcgaccatt atatgccatt tcgctcgcga
    34981 cttcccagga gacgactccg aattgatcga tcgtctcttt ccgaacgttg acgagattac
    35041 atttaacttg cggcgtataa gcgacgatgc ttccacaaaa aacatcgctg gccccgggaa
    35101 tgtcggtgac ggctgaggca aacaagccgc cggtcatcga ttcaacggcg ccgatggtga
    35161 ggttttttga cttaagaatt tcaacgagat tttgagcttt catctttatt ttcctccctt
    35221 aaaacttggc gattcgtaac gacatagata atacccgata gaagcgagac ggccgtcgct
    35281 aggtagacga gcagttccgc cactcttaat ccttctggaa agcctgaatc gaagtaggaa
    35341 aagggccacc cgttgagtaa aatagcgacg attgcgacca tttgcagtac ggtcttggct
    35401 ttgccaaaga tgttagcggc gatgacttta ttctttgtga cggcgattag gcgcagggca
    35461 tcgacgatta agtcgcgagc gatgagaata atcgcacacc ataaagaaaa agtgagcgtg
    35521 ctcggagcat aatgaggtgc gacgataaga aagatgagca aaccatcgat cagcagctta
    35581 tcggcgatcg gatctaaaaa ctttcctaag tcggtaatct gattgttgcg acgggccaga
    35641 aagccatcaa gccagtcggt tgccgaagcg agaataaaaa caatcgcggt gataaaatag
    35701 accagattaa tgccggtatt accaaggttc ggaacaccga tattaagcag agataaaata
    35761 aaaagagtaa tcagcattgc gacgacaatt acgattcgcg aaatagtaat cttcgttggt
    35821 aaattcatac ttctcctaag tgagtaccga ccctgtaagc catgttctgt caaggataac
    35881 aatttatcta tgcattatgt gtgcatgcgt accgcgattc gcttctctag gtacacttcc
    35941 cctaccaatt ttgggtttct cgcttgtggg gtttaccgcg ttccacctta gcgtttccgc
    36001 taagcttcgt cactgtggca ctttcaggga atccaccgta gccgaggctt taggtatttt
    36061 cccgccgtta tgcgctcccg catacctagc gttatttttt ccgctagcgc aatcactact
    36121 ggcatcacag ccagtgcgag catggacttt cctctggaat aaccagcagt tatcgaggtc
    36181 ggtgctattt aattttagat gggtcaaagc ctaaacgata caattctttt tccaaagcgg
    36241 cgatgcgccg aatgtattcg acgttctgaa ttgacacttt gccctcatct ttaatatttt
    36301 caagccgctt gcgatatttc gcgttttcaa tctccaaagc ggaagtctga tcgcgatgct
    36361 ttttaatgag aatctcgagc ttggcgatgt aatcatcgcg ctcaagcaac actttttcaa
    36421 aagcgagata gtccagagcg acttgatcga ggaatttatc gacttcgagc gcatcataac
    36481 ctttgacgtt tttggaaaac ggtttgttca ggatcgtttt cgatgttatt ttggctttga
    36541 aatccacgat atcttcctct tgttttaatt atattaaagg cacggctcaa ttacaattga
    36601 attacgaggc aaatcttcct ttttccccta tgctaatgaa ctccatgatg ctatactatt
    36661 tgcgaattat atgatcgagt taaaaaaggc aattcgcggt cggaaacatc tcgtctttct
    36721 ggatttagaa ggaacgcagt tttcgcatga gatgatcgcg atcggcgcag tccgtgccgt
    36781 gttaggcaag gatggaacga ttcgaaaact ctaccccgga tttaagcgtc ttgtcttggc
    36841 taagaataat atcggtaagg tcgtcgtcca gttgacgggc atcactaacg agatgctaaa
    36901 aaaagacggc gtcacctatc gcgtcgcgat ggaagatttt aagaaatatg tcggccgctc
    36961 tttcaccaaa tcgctattta ttacttttgg taaccatgat ctgcgcatca tcgcccagtc
    37021 ggtcagccac aaccttgacg ctaacaaaaa tgatgcaaaa ttcatcaaag agaattactt
    37081 agacctcgct gcttttatca gcaaatatat aaaagacgaa aaaggaaatt ccctctcttt
    37141 ggataattcg ctgaaaatct tcaacattga attcgacgga acaaagcacg atcctcttga
    37201 tgatgcgcgc aatctcgctc gcctttatga tgcgatgcac attaacccca atcttctcgc
    37261 tcaagaatat aaaaaagtac tcgctaaaaa taaagtgctc cccgcaccgc ttcccaagct
    37321 tattaataag ttgacgaagg gcgaatcggt tactcctgat gagtacgagg acatgattcg
    37381 cgaatacgtc agttaatatg attcgatatc cggatggctc aagccctcag ccaaaacgcg
    37441 aaaaggccat taaaagaccg cgcgctcttt cgagtgcaaa tcgcggcatg aatcttgagg
    37501 aagacatcaa catttctaat cagtattaca tcgataaaga cattgcggtc atcactaaac
    37561 gcccgacccc gatcaacgtc gtcaaggtcg attacagccg tggtgcaaaa attaccgacg
    37621 cctatttcga aaaacagtca actaccgact ataatggtgt attccgcgga aagtatctcg
    37681 attttgaagc aaaaagcact aaattgcata cctcttttcc attatctaac attactgccc
    37741 atcagatctt acatctgcaa agagtcataa aacaaggcgg aattgcattc tttatcatca
    37801 ggtttgaagc cctagatgag acttatctgc tcgatgcgag ttttgtcatc gaggctttta
    37861 gcggtgaaaa gcgcaaatca attccttatc cagacgttaa aaagaacggc catttaataa
    37921 aaaaaggctt tattccaaga cttttttatc tcgaagttgt cgaatcagtt tatttttgaa
    37981 aaataattgc aaatactgta taagtcgcat ttgtcgcatt ttggtttgcg ggcatggcat
    38041 atctctcttc caaaggtgat aatctggtga tttgttttga ctaagtcctc gggttttaat
    38101 agttttctaa gcttagtctc aatttgatca ggattgtctt tttgtttggc gaagccgagt
    38161 cttttcgaga tgcgctcgac gtgtgtatca accgccatcg atggaatatt gaatagttcg
    38221 gcttgaatga cattccgggt tttgttgccg acaccgggca agcgcaacaa gctggcgcta
    38281 tccgccggca ccctgccgcc gaattcatca gccaacatcg cggcaatgcc tttgacgctg
    38341 acggctttat tgcgatagag accgattgag cgaatggctt ctccgatttc ttctaaggag
    38401 gcctctttaa gcttctcgag gctattaaat ttggaaaaga gaatcggcgt gacgagattg
    38461 actgctttat cagtcgtctg ggcgcttaag acaacggcaa ttgccagctc gtaatctttt
    38521 tgatagatta actcacattg ggcatgagga aaattatcat ctagatattt cttaaaaatt
    38581 aagattttct cactattttt catcgtcatc gacccatttg gtcttggcga tttttatcgt
    38641 ctgttcgata tctttattcc atttttcact gacggcgcta taaccttctt tttccatgtc
    38701 atcgcgcgtc tgccactgca aaagaatttt atcaatcgat ctcagcgaac gcttattctt
    38761 gctaacggct tctttgaggg cgttgatgat catctcatcg ctataaccaa aattgaccca
    38821 ttcattaatg attgagaatt cgaggggaga caaggtgcgg tttaattctt tttcaaaaac
    38881 ctcatagata tttttcaaag tcgcattacg agcagcggac gaattagttt cctgctcttt
    38941 tgccaaagca agttgaaact ctttgtaaag ttttgtgcgc aatggtttaa gtgaagtttt
    39001 aatatttttg ttcgttgttt cgtattcgag gagccctcgt gtgatgagcc gcactaaaat
    39061 cttatcgata acttccgttt taagtgtcat gcgaaggcct aataaatcgg ccgtgataaa
    39121 tttatttttc tgctcgagga gatggtcgat gactaaaatg accgataact cattctcgtc
    39181 aaggcctatt tttttataat gttcgatcag cagatagcgg aagtcgagag cttccattga
    39241 gatattatcc atagtatcct ctacgtaaaa atatcatacc acgctaagcg tgtcggatga
    39301 catcactttc ctttgttttc ttcttcgcga actttgtcgg gattaagttt agcgagaaga
    39361 tggcgatatt tattcatggc cgccaatgat ttttcttcga tgataaagcc gagttttctg
    39421 gcgaaccgct cggcccgcaa aatgcgcaga ggatcttcga tgacacgctt ttccggttct
    39481 ccgataaagc ggatgattcc ttcttttaaa tctttcaggc cgtcgacaaa atcatagatt
    39541 ttaaagtcct tatctatgta aatggcatta atggtgaaat cgcgccgaat atagtcgcgc
    39601 ttgatatcgc ggacatagcg gatcgttttt ggatggcgaa aatcgatgta atcggcttct
    39661 tcgcgaaggg tgacaatgtc gattttctgt ttgttataga aaagagatat cgtgccgaat
    39721 ttagcaaagg tatagctggc cgcatcaaga aaatttttca tttcttcagg tgttgcatcg
    39781 gtgacaaaat catagtcgag aacttcgcgc tccaggagat agtcgcgtga tgttccgccg
    39841 atcatgaata aggaaaaacc atgtttctca aagagtctgg caagattaat aaaaatgctt
    39901 ttatccatag ataaattgta tacaaagatg acaaataaaa aaaggtcagc tttcgctaac
    39961 ctttggtgtg acaataaatc cttaattaac cttttctttt aatgattttg aaggcttgaa
    40021 ggatacggta tgacttgccg gaatttgaat cttcttaccg tcagtcggat ttgtaccggt
    40081 gcgggcagcg cgttttttgt tctcaaacac gccaaaaccg gatagtttga cggcttgttt
    40141 tttaacgagg gctttttcga ctagttttaa aaaactttcg acggcagcct cggcttgacg
    40201 tttggaaaga tcagcctctt cggcgacagc taaaatcaaa tcagctttgt tcatagattg
    40261 ttctcctttg ttttctgtct aaaaattatc acagcatcaa ataattatca agattattgc
    40321 ctactaatct tcttttttaa gaggtcggct cattagttcg gtaattactt ttgacggttt
    40381 ggcatcttca aacaatactt tgtaaatagc ctcgacaatc ggaagggata gaccgcttga
    40441 agaagctaat tcgtgaatta ctttcgccgt tctcacgccc tcgacagttt taatattttc
    40501 ccgatgaaag cgctgactcg aatcatcgct tccaatcata aatccggctt ggtagtttcg
    40561 tgagtgctta gagccgcatg tcactaccaa atctccgata ccggttaaac ctaaatacgt
    40621 tttcaaacgc gcattatagt gcttgcccag ttttaccatt tcatttaagc cgcgcgttat
    40681 taaggcggct ttggcgttgt ctcctaaacc taaaccatcg attattccgg ccgcgatggc
    40741 gatgcaattt ttcattgccg tcgcgtattc cgcaccgatc tcatccttta ggatgtagac
    40801 gcggaaatag cgattagaaa ataatttctg aacgagctcg gcgtattttt tgttacgcga
    40861 cacggcggtg atgagcgtga ggtgccgcaa gatgacttct tcggcatgcg aaggccctaa
    40921 tagcgagacg acgccagcga gtttatcttt gctaatatga tggcggatga cttcggacat
    40981 tcgctccttg gtgtccgggt caaagccttt cgcagtatta acaaaaagcg tctttttact
    41041 aagatgcggc aacattttaa tcaatacatc ggaaaccgct ccggtcggca cgctcaagag
    41101 aatcaaatcg ctatcgcggc agatgtcttg gatggagttt gtcgccacga tgctcgggga
    41161 aaggttgatc aaatcgccaa aataaaaaga gttgcgatga ttgagattga tgtcatcaac
    41221 ctggttttgc tcacgtccat agagaattac tttgtgacca ttatcggtca gaacctgcgc
    41281 taatgccgtc gcccatgtgc cggttccagc gatgccgatt cgcattttta cttgcgctcc
    41341 cggagaatga ttttgatcgg cgttccatca aaagaaaatg attcgcgtaa gcgattttcg
    41401 atgtagcgaa gatatgagaa gtgcgcatat tttggacgat tgacgaacaa gacaaaagtc
    41461 gggggtcggg catcgacctg attagcgaag taaattctta atcttccgcc ttcaaataaa
    41521 ggagccggat tcatgatttg tgcatcttgt aaaatgtcat ttaagacact cgtctgaatg
    41581 cgtcttccgt atgcttcata aacgatatca agggcttgaa agacggtgtc aatccggctt
    41641 ttatctttcg ccgagacata gatgacgggg gcatattcca gaaatttgaa ttccttgcga
    41701 attttcttgg taaaatcgct catcgccgtc tgcgagcgag gaactaaatc ccatttgttt
    41761 actacgagga tgatggcttt attgagctcg acggcataac cgacgacatg tttgtcttgt
    41821 tcggtaatgc cctgctcggc atcgatgaca agcatgacaa tttccgatct ttcgattgcc
    41881 gataaggcgc gcaaagcggc gtatttatca atggcttcat aaatctttcc gcgcttttta
    41941 aggccggccg tgtcgatgac gacatattgt tttccatcgc ggatgaattt agtatcgatg
    42001 gcgtctcggg tcgtaccttc aatcgcggag acaataacgc ggttttgacc taaaatcgcg
    42061 ttagataatg aagatttgcc gacatttggc ctgccgataa ttgaaaaaac gatttcgttt
    42121 tcttcgcgtt tttcaggttc cgattccgga agcaatttaa cgatttgatc taacaaatcg
    42181 ccgactccga tactgtgagc gccgctgata gcgatcggtt cgcccaagcc taaagtcgaa
    42241 aactcataga tatcggcgat tttgcttata tcatcaattt tattaaccgc aagaataatc
    42301 ggcttattgg atttatagag cattttggca atctcttgat cgtcgttggt gacgcccact
    42361 tgtccgtcga cgatgtagac gatgacatca gcctcatcga tcgccatttc agcttgcgcg
    42421 cgaatttgtt cttgaaaagg gcgattggca atttcgatgc cacccgtatc gatgacgcga
    42481 aattctttgg tcagccaagt tgcatcggca tatagccgat cgcgggtgac gccgggctca
    42541 tcatcgatga tggagcgccg cgtgccgatg atacgattaa aaatcgtcga tttcccgaca
    42601 ttcggactac cgataattgc gactacgcca agaggcatat tattatgcct ccccgatttt
    42661 gcgtttgatt atatcaagaa ctgcttcgac ggcttcttca atactaagaa acgaagtatc
    42721 gagaagaacg gaatcggcgg ctggttttaa aggggcaaaa tcacggtgcg aatcaatgta
    42781 atcgcggcgg cggacatctt cttcgacttc cgccagcgta catttgatgc ccttggaaat
    42841 attttcttca tagcgcctga ccgcacgagt ggggacatcg gctatctgaa atattttgac
    42901 atcggcatta ggtaagacat aagttccgat gtcgcgacca tccatgacga ccgaacggcg
    42961 ctgagccatc tttctctgaa gttcgacaag ggcgagccga atatctttgt aagaagcgat
    43021 gtaagagaca ttagcagaca cttgcggttc gcgaattact ttcgtaacgt cttcgccaca
    43081 gcagatgacg cggccatcgc tatgaagttc gatatcgacc tcgggaacaa gcgaaatact
    43141 ggcggcttca tccttaggat cgagaccttt tttcatgacg taatatgtaa aagcgcgata
    43201 catcgcgccg gtatcaatat atgtgtagcc gagagcctgg gaaacttttt tggcgacggt
    43261 tgattttccg gccgcggctg gcccatcgat ggcaatcgtg actttcttca tgaagttctc
    43321 cttttttaaa acgcgttaat tccgatgatg attaacccga caatcaagag tataagaatt
    43381 gagataagaa tgattgtctg aatcagttct ttgcgttttt gtttgacgat cggtgagacg
    43441 cttttgcgct cgttttcgtc tgatgcccgc acgctttcaa taatgtcgtc gatgccaaat
    43501 tcaatcgtcg agcgcgcgct cttgatatcg ctgacctgtg acttcttggt ttgggcgatt
    43561 tcgtaaccga ctggtttttg atcactcgaa agttcgctat catctggcat gcgagagatt
    43621 tcgtcgcgca gcttctggaa tttttcgacg cgggtgagag ctttcaatgg tcagtcctgc
    43681 ttactgatat attctaatat aaattagaaa tcatttatag ggtaatttga taaaccttgc
    43741 ataaactatt gtttaatatt ataattcccg ttgttggagg acactataaa aaatggcgaa
    43801 aaaagttaga attgaccgcg atgcttgcat cggatgtggt ctctgcgtct cagtgatgcc
    43861 agaagtcttt gagtttgacg acgagggaaa agcccgcgtt cacgctcagc ccgccgaagg
    43921 cgtcgaagtc gaagttgcga atgattgccc gaccggcgca attatcgtcg aagactaatc
    43981 gtcgattctt ttattaaaag ctgcctatcc gggcagcttt ttttgttcga actgagccat
    44041 gaagcgatgg atgctcttgt atacgagaaa agtcaggatg acgaccatcg cgtttttaat
    44101 aatattgaaa ggaacgatcg ctaaaaaccc aagtgaccag ccgatgtcgg ttaccgcggg
    44161 attcgcagcc ttggacatcg ccaaaatcgc cgcctcggga aaacccatca cgaacatata
    44221 aaaagggatc atgatataga cattaaacac gaggctggtc gccacttgca cgatgaagcc
    44281 gacggcaaaa ccaagaatga cgcctttgat attacgcttt ctttgataga tgatggccgc
    44341 gggaatgaca aaggctgtgc tgtagattaa atcagcccat tcacccactg tcagtgtcga
    44401 ggtgaaagga agttttataa tagtttttac tattattgcg caaaaagcac taaatggccc
    44461 gtaggcaaat ccggcgatga ataaaggtat ctcgtcgaag tgaaattcaa gaaaagaggg
    44521 aaaaatcggc aaagggaatt ttaagaaggg aacgatatac aaaatcgctg aaatagcacc
    44581 gaaaatcgcc acgcgactca tgaagcgaat gatgtcaatc ttttgaaaat gaaacgtctt
    44641 tccttccctt aaaacaatta tgagaagaaa gatccatata agtaataaac cgacgactgc
    44701 tgctcccata aaaaaggtcc ttctttcagg accaaaatga gatacttctc tcatccagac
    44761 tgcactgtcg cctctagaat ttaactagat cggcccgaag gctcgcgggg tttaccgccg
    44821 gttgggaatc tcaccctgtc ctgaagcttt ttctattata ctacaaaata caaaaacgga
    44881 agcaaaatag cgattgaaag gttgttattt gccgcttctt agaagcgctt cgctgatcga
    44941 gtcgatgatg tcggaagcca ggaggctgtg ttcgccacgt ctttcacaag cgattcctcc
    45001 ggctatctga tggagataga cgccatactt ggcggcttca agaaaagtat aattctggcc
    45061 gagaaggctg acgatgatgc cggccaaaac atcgccggaa ccggctttag ccataccggg
    45121 attcccaaaa tcgagttgac cttcatattg cttggcgtag acagttgtca ccgcgtcttt
    45181 taagacgacg gtcgctccga gagaagtgag cgtgtgtatt tccttatgcg gttcatgact
    45241 atcaagaagt cgttttgcct cgcccatatg gggggtcaaa atgacatggt caaggcgctt
    45301 atttaaaccg acttttttaa gaattgtgag tccggtggca tcgattacga ggggaatatt
    45361 cttgcggaga aggagtttta aaatgcgctc attcatcagg ttatcttcta ggcccgggcc
    45421 aaacaatacc gcattcttat tctcaagcgt tttttcgact tcttcaatgt cgataaagcg
    45481 cttaatcatc acctcgggat tgagattgac atacagtgaa taatcatctt cttcaagcgc
    45541 gatcgagacg agtcccgaac cggtccgata agcggcgagg gcgctgagcg ccggagcgcc
    45601 aaaaaagccg atgctgccgc caaatatcac gatgttccca tagtcgtact tatttgtttg
    45661 cttgcttctt tttggaagtg gcaagagctc atctttcgtg attttcttca taatttctcc
    45721 actaactatt tttgattatt gcgaattttt ttgatgatca tttcttttga tttcatcacg
    45781 cgaattgtgc cggccatctc ctgatcggca agcttggttt ttatgtcttt aatcaaggcc
    45841 acctgctcgg gaatcatgat gtgttcaatc gcattgacgc gccgcctcgt cttttctatt
    45901 tcgcgggcaa aagtctccgt cgtcttttcc agttcggcaa gggcgatcag ctttgggaga
    45961 agttcaagac cgagcatcaa agcctcatcg aactcctgag gagcagagct tagggcatag
    46021 gggagttcgg ttttgccatc aaggctaaat cgcagatgcg gcgattcgac gctcatcgtc
    46081 atcgacttgc tcgtttgtaa agaccaggaa aaagccggaa ccgaagtgag ttcatacaat
    46141 tcggcttcac gcgttttcgc tcgccctttt ttaaatgacg aaagggcttt ggaagtcatc
    46201 gtctcgacct cggttcttaa atccttggtg cgatagacta tttccatgaa ctggcgaatc
    46261 aattcatctt gtttatcttt taaaagatga tggccgcgcc tggtgatggc gagctgattc
    46321 ttgagctttg tgagctccat tctggtcgga ttaatttgac cgattgccac cgctattcac
    46381 ctgttccgta aaattcggcg atgttttcgt cgctgatgcg ctttagctca gaagtcggga
    46441 agatgcgaag taattcccag ccgatatcta aagtctcttt tatcgggcga ttggttgtaa
    46501 aaccctgaga gatgtatttt gcttcgaaat cctcggcaaa tcgcgcataa agtttatcga
    46561 tatccgatag cgcgctatcg cctaaaaccg ttgaaagttc cttggcctcc ttgccgcggg
    46621 catatgaggc aaacagctga ttcatcgtgt cggcatgatc gccgcgcgtc ttgccttcac
    46681 caatgccttt gtcctttaaa cgcgagaggg atggtaagac atcgatcgga ggaaagatac
    46741 ctttcataaa caagcttctt gaaaggatga tttgaccttc ggttatataa ccggttaaat
    46801 cgggaatcgg atgcgtttta tcatcttccg gcatcgtcaa tatcggcagt tgggtaatcg
    46861 atccttgaat gcctttgatg cggcccgctc tttcaaataa ggaagcgagg tcggtgtaga
    46921 gatatccggg atagccgcga cggccgggaa cttctttgcg ggctgacgaa atctcgcgaa
    46981 gagcctcggc gtaatttgtg atgtcggtca aaataactaa aacatgcata ttaagttgat
    47041 aagccaggta ttcggcggcg gttatcgcca tgcgcggagt ggcgatgcgc tcgatggccg
    47101 gatcgttggc gagattaacg aacatgacgg ttctttcgat cgcgcccgat ttacgaaaat
    47161 cttcgataaa ataatcggct tcttcaaaag tgatgccgat cgcggcaaag acgacggcga
    47221 atttttcgga ttttccgagg actcgcgcct gcctggcaat ctgcgctgcc agttttgaat
    47281 ggggcaaacc cgagccagaa aaaatcggca gtttttgtcc gcgaaccaga gtatttaacc
    47341 catcgattgc cgagatgccg gtctgaataa attcagaggg ataatcgcgg gcgacaggat
    47401 tgagggcgga gccgttaata tcgagtctgg tgtcggcgat aaccgctccc ttgttgtcga
    47461 tgggacggcc gagtccgtca aagacgcgac cgagaatgtc gggcgataag cctaattcga
    47521 tgccgtggcc caaaaaggtg gcttttgcgt catcgatttt catcccttga ctcgattcaa
    47581 atagctggac gagggcttta tcttcattaa tttccaaaac tttgccgagc cgcgtttcgc
    47641 cattcgcgag tttgatttcg acgagctcgt catatttgac atcttgaacc ttctcgacga
    47701 gcattaacgg cccgacaact tcttgaattg tcttatattg tttgatggcc ataattactc
    47761 ctctctcagg gatgaaaatt ctttttccat cgcttgttca atactgtcgc actccttttc
    47821 cacatcagct tcgcgaacat atttaattct tccgatgcgt tcgggtgttt tcatcttttc
    47881 aatctcgcta aaagccttgc gaccttcaag cgcttttttg gccagatcgt accaccgcaa
    47941 aatcgttttg agcatgcgat gctgtttagc gattgaagcg taagtatcga tttcatggta
    48001 cgcgttctgg tgcaggaagt cctcgcgaat catctgcgac gccaaaagtt tgatgcggtc
    48061 gctttttgat agcgagtcga taccgactaa acgcacgatt tcctgaagcg atgcttcttc
    48121 ttgaagaagg acttgcacgc ggcgaatcat tttgctccaa tctttgtgat aatgagaatc
    48181 gatatcgaga tgtaaaacat cttcatataa gctataactc ttaatccagt cgatggccgg
    48241 aaagtgccgg cgataagcga gctgagcaga gaggccccag aagactttaa caatccgaag
    48301 cgtcgcctgc gagacgggtt cggatgtgtc gccgcccggc ggagaaacgg ctccaatcgc
    48361 ggtgacggct cctcgacgat ttttatcgcc gagacaggcg acataaccgg cccgctcata
    48421 aaactcggcg agacggctcg atagataagc cggatagcct tcttcgcctg gcatctcttc
    48481 aaggcgcgag gacatctcgc gcaaggcttc cgcccaacgc gaagtagaat cagccataat
    48541 cgcgacttta tagcccatat cgcgatagta ttcggcgatg gtaatgccgg tataaatcga
    48601 agcctcgcgg gctgcgactg gcatattcga agtattagcg attaaaaccg tccgcttcat
    48661 caacgactcg ccggtgcggg gatcttttaa acgcgggaat tccatcaaga catccgtcat
    48721 ctcattgccg cgttcgccac accccacata gatgatgata tcggcatccg cccatttggc
    48781 taattgatgc tgaacaacgg tctttcctga tccaaaagga ccaggaattg cagcaattcc
    48841 gccgattgag acaggaaata gagcatcgat gacacgctgc ccggtaacca ttattttatt
    48901 cggcgcgatt ttgttgagat atggtctttt cttgcggacg ggccagtact gaatcatcgt
    48961 cagctcaatc gtcttgccgt tgttatcgac gatcttggcg atgacatcgg tgactttagc
    49021 cttgccttta tgaagccaga caagttttcc ggaaacgctg ggggggacca tgattttgtg
    49081 attgacgacc ggagtctcct gcacggttcc taaaataacg ccaccgctga cttgaacatc
    49141 aagttgatgg acgggaacaa aatcccattc gcgttcgcga tttagacgag gtaaatcaat
    49201 ccctaagggg atatgcggac caaaggagtc gtaaatattt gttaacggac gctgaatacc
    49261 atcaaaaatc gattcgatca aacccggacc taattcagct gacagaggtt tattcgtata
    49321 gaaaacgggc tcgcccggac cgatgccggc tgtctcttcg taaacttgaa ttgaggccag
    49381 accgccacgg agctcgatta cttcgccgat cagtttttta tgcgcgacgc gaaccacatc
    49441 gtaaacttgg acattttcca tgcccgaagc gacaaccaga ggtccggcaa cttttgtaat
    49501 tgtaccaatt ttttccatat gaactccttt attaaaataa ttttagacca atggcttttt
    49561 cgacatcggc gcgcagtttt tctaagcctt gcccgctcgc ttcatgatcg atggggaggg
    49621 ccatgatgat agggaaggct ttttcgttaa agcgcttgcg atactcgcca atcatcgcct
    49681 gtaaatcttc atcgatgatg atgatctgag cttcggccgc aatgctttcc agcacacttt
    49741 tcatcttctc ttctccatcg atgacaaacg tttcgaatcc taaacttcga aaaagaaaaa
    49801 cattctccgt tttagcgatt gcgacggcgc gcttattcat gtttcgtcca ccatcgccgc
    49861 cgatgctcga tcaagatgga tgcggcgaat atcaattagc tcaatctgct tgcgaatgac
    49921 atagatgatg atatttgcgc tggacttgat atcgacagca tcgcgagtta actcatcgac
    49981 gagaaagcgc agcaaagcgt gctcaagaga cgagaaattt ccgtcttcga aaaacaggtc
    50041 aagaggttcg ctaaagcgag ttaggtaagc cgtcgcatag aaggaggcga tttcgcggtc
    50101 ggaagcttcg acgaggtgga cgatgtcagc gatgctgatt gaaccgcaat ctaaaagatt
    50161 gtttttgact tgttccgcgt ccgatctcag ctttcgggat ctgagcaggg tgatgagatt
    50221 attgatatcg gttgaaataa caaagtactt ttctaaagag gcatcttggc gagacttgat
    50281 ttgttcatac attaatttct gaaaagtttt ttctaaaaaa gtcacaagcg aatgactgtc
    50341 gctaaatgtt tgattgttgg cttcggaaat caaatgcttg taaggttctt gaagattgta
    50401 atagtcattt ttcagaatag cttgagatag ttctttttcg gttagaaagc cagttggctc
    50461 aaaaggtccg agctcggcct gaaacaactt ctttttatag taagcacgaa tattcgtcag
    50521 atcgtacttg gtaaaaaaat acggcgccaa atcgtcgttt ggcatgatct cggaaagttc
    50581 tctttttagt ttcaaaactt ctgcgccgac aattgcttcc accgctttcg gctgctcgcc
    50641 cactccgtag ccaaacgact gcagttttaa aataaacccg tcatcatcgg catttcgcaa
    50701 ttcacgaaat tgagccttat taagaaggct ggcagacttc gtgctgagca aggcgctggc
    50761 gaaagtatag tcagtcatca ttctccttca ttttcaaaaa gcgatgcgaa taactctttt
    50821 tctttgcgtt tgcgcaagcg gtcaagaatc ggcttgacgc taaaattaag atcgtaagtg
    50881 tcgcccagca gcaggaaacc ttcttcttcg tttgaagaaa catcttccaa gacgagatga
    50941 tagtcttttc caagcgcttt atttaagcga tcgaggacga cattatgagc gagatcttgc
    51001 gatgaaagag cgaggcggta gcgttcataa tcgcgagcgt tgacacgcat tatttcatcg
    51061 ccttttatct tttcgtcctg aatttgtttg acgacaaaag acagaagatc cgaacctttc
    51121 agacttttga agtgatccaa gacagcacta aaaatctcat cgatggactt attttttaaa
    51181 gccgccaccg attgcctttt ctcgagttcg ctgagagctt cagcttgggc gattgaagtc
    51241 gttttactct catcagcttt tgcgagcaaa agtgccgctt cgcgttcggc ttcggcgacg
    51301 atggtgtgtt cgattgcctt ggattcgcgc tcggcatcct ggcgaattga atcgctttca
    51361 gctcgtcctt tattgatgat tttatcgtag attgacatgc atgtgctcct aaacggcgac
    51421 cgcagcgtac atgaggatgc tgaccaggag ggcgagaatg gcgtaagttt caaccaaggc
    51481 cgtcatcgtg atgccgcgcg ttgccatttc gggacgcttt ccggtcatca aaatcgacgt
    51541 cgcggccact ttgccctggg ctattcccga aagtaaaccg acgagggcga taggaatggc
    51601 ggcgccgaga aaagcccagc cctgccaatc atctaacggg acggcttgac cgcctaaaag
    51661 gccgaccttt tgcaaaatta aaaccgccat caaaaagccg tagatgccct gcgtgccagg
    51721 caaagcctgc aacaccaaaa cacttccgaa aagattcggt ttttcagcca ggactcctgt
    51781 cgctgaacgc ccggcgatgg aaacgccgac agccgagccg atgcccgcca aacctgcagc
    51841 cagagctgcg ccaataattg atagaactaa tccgtaagtc atgattctat ctcctttctt
    51901 ctcgtaaatc cgagatttca ctgatttgtt ttaaacgaat gctaaagggc tcaaataagt
    51961 atcctccgcc ctcatagaac tttccgaaaa actcgatgta ttgtaaacgg ccgtcatgga
    52021 catacgccga gagcatgctc atcacaaagt taaaaacatg gcccacgaga tagacgagaa
    52081 gagacaagat gaaaccgatg acactgcctt gaatcatgcc ggcgaggagg ttcatcgtcg
    52141 aagcgatgac cgccgaagag aggctgaggg ctaaaatgcg cgaataagac aggatatcgc
    52201 tgacataact cgtcgactta taaagggctc cgagcccgcc gaggactttt ccaaagatat
    52261 ttttgctttt gcgacccgcc aagaagagga ggaggagggc gccgacaccg gcaaagccga
    52321 gtccgatata gctaagcaaa gaagccgatg tccagatgac taattgcgcc gcaaagagga
    52381 cgaggccgac caggatgagt atccagctca aagcgtcggc gatggcaccc acataatctt
    52441 tgcgtctgat cagcaaagcg aatttaaaac cgaggccgtt gatgatgtga aggacgccaa
    52501 acaccaatga gaaaccgagc attatcaaag gatcattgac agggtttaga ataattgtcg
    52561 tccactgctg agcaaaaagg ctgccgatga gggcgcctaa atcaaaaggc gcaccgaaga
    52621 aactgccaaa taaaataccg gcgacaatcg aggtgatgcc agagtaaaag aaaacgctga
    52681 ccaattgttt taatgtgcct tttggcttaa taagccaaat agcaagtcca aagatggtta
    52741 acatcgccag tccataaccg atgtcaccca tcatgatgcc aaagatgatc cagtaccaga
    52801 tagcgacggc cggattagga tccagctctt tatgatgcgg cacagagtac atattcgtaa
    52861 tcgtttcaaa ctgggtaacg aatttattgt ttttgatagc cgtgggtgga agatccccat
    52921 ctgtcggatc actcaattcg atgtcaaagt cagttgtgac ggctttgacg gttttgctta
    52981 attcgtccac ttggtcagcg cgaacccagc cgtcgagata aaatgatttc tcggtcttag
    53041 caaaggcgac gcttttacga tcgattttgg cctggatgcg atcatacatt aactcaagca
    53101 ggcttttgct tttagcgaga tctttaagac cgatttcaag atcgccgact tgcttcttgc
    53161 gtgctgatag gtcgttttcg agattgctta catagtctga catcgtgagg ttgacatccg
    53221 gcaaatcaac ttcgctgaat gagcgcttat tgacttcgcc gagcagttgc tcatcctctt
    53281 cataaaatga agcaaaaaat agagccgttc catatattgg cgaggaaaaa gtttgatatg
    53341 gaatttcttt ttcggcaaag aaagccttta aattatcaag gctatcctcc tggacatgac
    53401 cgacatggta gcggctaaaa gatgatttct ttaaatcaga cacatttata tcgatttcgc
    53461 ggaaaggcgc atatcgttga aacgactctt cggttttctt aatgtgcgaa agaaggctgt
    53521 tgagattttc gattttagtt tcaacatcct ccaacagccg gagttgccgc tcatcttcaa
    53581 gcatgaaatt ttcataagtt acttcgtggt gtttaaagag agattttttc tttccatagt
    53641 gacttaaatc attgatagct ttattgattc tttgtaaaag cactgtatcg gacgtcacgt
    53701 cgacgctcga agaggaattg gggacaatca ttaaaagtga cgccttttgc aaggcgcgga
    53761 ccaattcgtc tttgcgctct tctaaaacga agatgcgcgc tttcttaacg ggaacgatca
    53821 ctggacgtta cctcttgaat caactgactg atcaaatgat ctttcttgcg ggcggtcttt
    53881 tttttgatat tctcttcaat ctcttttaaa tcgttggcta atttttcctt gatttcggcg
    53941 atttttgcgt tcgcctcagc ttcttgcttc ttgccgagcg cctcagcttc attaataagg
    54001 gacgcagctt cttcttccgc tcttttcttg gtttgattaa tgagcatcgt tttgtagacg
    54061 agcgcctccg tgacaaggct tgctgcttgc cgttcggctt cgattatttt cttcattgct
    54121 tccacaaaat tacctcactt atccatagat taaatatcga attagtattt tagcatattc
    54181 acaatgttat atctatataa cttcctagga agacaaatat ttaaaaagct agttattgaa
    54241 tccggctgtt agaaataagt ttggtaaaaa tattaaagaa taaggcattg tgaaaatccc
    54301 ggatatcgag atttgtcgcc tcgatgaatt gattgagccg gtaagcgaag gtattgcgat
    54361 gaacatatag ttttttagcg gccagggaag cattgagtcc gcacgagata aacatctcgg
    54421 cagtgaccat caaagggcgc gaaatcgccg catattgtct tttggcaagg cttatcaatt
    54481 caaagtcatt ttcaagtgcc aaatttaaca agatgtcggc catggtgctg agatggacgc
    54541 ctcgactttt agtcatcttt ttcatggcgg tccggctcac ttgactatcg tcgtggctca
    54601 tcaaaaattg atacgaatta tccgtgtcgc tcatgatgac gggaataagc gattccaaat
    54661 aatcatacaa gtgctcatcg gataaaacat aaccgcttcg accatcaaaa aaagtgattg
    54721 aaaagatgtt tttatacgag ctgacgagcg cggcgttaaa acgctcggca ttaatgtctt
    54781 tctgcgatgt gaaaaaatag tattttacca taaatcaatt attgtgcttt atgcacaaat
    54841 agtaaagatt agttaacgaa taaattcttt atccatcact tcgcgggcca aggaaaaata
    54901 ggcgaggctt cccgcggaac ttgggcgata aagcgttacc ggcagacctt ttgaggaaga
    54961 ctcgggaatc gaaatattgc gaggaatagt cgccaaaaag gtattttctt taaatagagc
    55021 cctgatttgc gtggttattt ccgttccaag tctcgttctt gaatcataca tcgtcaataa
    55081 gaaaccctcg attccaagct tcggattaga agttcgctgg acttttgaaa tggctgcaag
    55141 tgtcgtcgcg acagcctcga gggcgaaata ttcgcactgt accggaatca atatcccatc
    55201 ggaagcagcc atcgaattaa gatttaaaat gccgagtgaa ggagggcagt cgatgacaat
    55261 atagtcatat tcttttttga gattttttaa cgactcgcgg agaacgagcg tcttgtcttc
    55321 gactttgtca ctcagcgtcg aatcaaggga ggcaagcttt aaactgctcg ggaggaggtc
    55381 tagatattta agagccgtgc gcttaatcac tttattgatg tcatagccat ttgccaggac
    55441 atcataaatg ctccggctca ggagcgtcga atcaaagccg agtccgcggg tcgcgtcgct
    55501 ctgcggatct aaatcaatca ataaaatgcg ccttttgaaa tgggccaagg cgctggacaa
    55561 attgattgcg gttgtcgttt ttccaacgcc gcctttttga ttggcaatgc cgatgatttt
    55621 ggtcatgatg tattagttta ccttaaatgg taagaagcat aaacaattaa agcggcttag
    55681 aacttatctg actgtagatg cgcggatagc gaaggggagt cgtatcgttt ttaacgataa
    55741 gaagattgac tctcaaatct ttatccgtcg gcaaaatatc ttcatggcaa acgacaattt
    55801 tggctttcaa gatttgcatg gcttttttcg aggcgatgat ttcttcgctc gccccctggg
    55861 atttcatggc gataaaaagg cctttaacct tcaataaagg gattgctaat tccaggagga
    55921 tgttcagctg tttaaccgcc cgggcgattg caaaatcaaa ttcctcgcgc aatgcatttt
    55981 gcgtttcggc ccgtccgacg acgatggtga cattatcaag atgcaaattg tctttaacgg
    56041 tctgtaagaa acgggctttc ttattgtttg gttctagcaa gacaatgttg gcttgcggat
    56101 tcgcgattgc caaaacgact ccgggaaaac cggccccaga accgatgtca atcagcgatt
    56161 tttccttaat agaaacatat tttaacggca gcagactatc caaaaaatgc ttttcgataa
    56221 tcttttcttc gtcggtgatg gcggtgagat taaacatgac attccactct tttagcaaag
    56281 ctaagtagct tgtaaacgcc cccatctgcg atgcgttcaa ggtgagattc aactcattca
    56341 tgcaatagtc ttgaaattgt tcaaagttca taatagtcct cgccttttta atgttaaaac
    56401 gagcacggaa atatcgctag gattaactcc gccgacgcgg cttgcctgac cgatcgtcct
    56461 ggggcgaatt ttttgcagtt tctgacgcga ttccaaagcg agtccgtcaa tatggagata
    56521 atcgaggtct tcaggtagaa gcaggctttc aaatttcgca aaagattttg cttccttttc
    56581 ttgcttggcg atatacccct catatttaac cattatttca agttgcataa tacccgtttc
    56641 atcaagagga tgaaatatca gttcagggat gagttcaatc atggtcgaat aagcgacatc
    56701 ggggcgcttt aataaatcat aagccagagt accgcctttt aaaggtgtac acccaatttt
    56761 aagcagatat tcattcactt ttttgttggc gcctaaatac gtttttctta agattgacag
    56821 aacgcgattc aagctttcat ttttttgcaa aaaactttga tatctcgctt cgggaataag
    56881 gccgagttgg taaccgtatt cagtgagtct taagtcggca ttgtcgtgtc tgaggagcag
    56941 gcggtattcc gctcgcgaag acaggagtcg atatggttcg atggcccctt tggtgacgag
    57001 gtcatcaatc atcacggcaa tgtaggcttc attcctcttt aagataaggg gtgcttgacc
    57061 gcgaattgcc aaaacggcat ttatgccggc catgatgccg agcccggcgg cttcttcata
    57121 tccggatgtc ccgcaaatct gaccggctcc ataaagacct cgatactttt taacacgcaa
    57181 agtcgcatcg aactgggtcg gaacgattgc atcatactca atcgcatatc catatttgag
    57241 aatttcagca ttttcaaatc cgggtaatga gcggaccatc tgttcttgga cgtcgcgtgg
    57301 catggaagtc gcaaagccct gaatataaat cgagtcgcca tcttttgtct cgggctcaag
    57361 aaagagctgg tgacgcggct tatcatagaa agtcgttatt ttgccttcaa ttgaaggaca
    57421 ataacggggt ccaacacctt taatttggcc attaaactgg gcggagcgtt ctaagttatt
    57481 tcgaattatt tcatgcgttt taggagaagt ataaataaga tggcaaaccg cctgattttc
    57541 aaaggccacg aattcttttg tcgtgtaaga aaaagctaac ttggcattcg tgccgtattg
    57601 aacgctggcc ttcgaaaaat caatcgacga cttgcgaatg cgcggtggcg ttccggtttt
    57661 taggcggtaa atttcgatac ccatctcagt gagatttttg gacaatccaa ccgaagcctt
    57721 ttcgccgtcg ggaccgcttg cggttttttc aagaccgcgt aaaatctgcg cttccatata
    57781 tgttcctgtc gtaataacca cagcttttgc tcttatttca gcgccgtcgg acatcaccac
    57841 cccaaaaact ttttcgttat cgtgtagtag ggatgtgacc atggcttctt taatttcaag
    57901 gttagaagta tttaacagta tttcctgaac gcggcccgga taaatattgc ggtcgctttg
    57961 cgtccttaaa cactggacgc ccggtccttt gccggtattg agcattttca tctgcaagta
    58021 ttgataatcg gcagcaactc ccatgatgcc gcccaaggcg tcgatttcac ggacgacgat
    58081 gcctttggct gatccgccga ttgacgggtt gcacgacatg cgagagacag ctttaaaatt
    58141 cagggtgcaa agcaaagttt tacatcccat agaagccgcc gcgtgagcag cttcgacgcc
    58201 ggcgtgaccg ccgccgacga cgatgacatc gaattccact aaccgacgct tccttccatg
    58261 tccatcttaa tcaactgatt aagttcgacg gcatattcca tcggaagttc ccgcgaaaaa
    58321 ggctcgataa aacccatgat aatcatctgt gtggcgtctt tttcactcat gccgcgcgac
    58381 atcaggtaaa agagttgatc ttcattgatc ttactgaccg tcgcttcgtg ttcgatatac
    58441 gactgatcat tgaaaacttg attggtggga atcgtatcgc tttttgaaat atcgtcgaga
    58501 atcagcgtat cgcactcgac atggctgcgc gagttgagcg ctcctttttc gtggcggact
    58561 gtgccgcggt agttggcgac tccgccattt cgtacgattg atttagatat aatcgttgaa
    58621 ctcgtattct tgcccagatg aatcatacgc gcaccggcat cctgatattg accatgatca
    58681 gaaacggcga tcgaaatgca agttcctctt gatccgtcgc cggataaaat aacggccgga
    58741 tacttcatgt taacctggct tccgatgttg ccgtcaatcc actccatttg cgcattatca
    58801 tggcaaatcg cgcgttttgt aacgagattt acaatgtttt ttgaccagtt ctgaacggtt
    58861 gaataacggc atttaccatc gcgtaaaaca acgatttcga caacggcggc gtgcagtgat
    58921 tcgcgagaat aaataggcgc cgtgcagcct tcgacataat gaacatccgc tccttcatcg
    58981 atgatgatca gcgtcctttc aaactgtccg gagcgctcgt tgttgatgcg gaaataagat
    59041 tgtaacggct tatcgagttt gacgcccttg gggacataga taaacgaacc gccggaccag
    59101 accgcgccat taagcgccga aaatttattg tcggcaaaag gcacgacggt attgaaatat
    59161 tttctgaaca gatcgggcat catttttagc gcttggtcgg tcgaaagaaa aacgacgcct
    59221 ttttcctcaa cctctttcag catgttgtga tacaccatct cggattcata ttgcgtggca
    59281 acgccggaaa gatacttttg ttcagcttcg ggaattccca acttctgaaa cgtcttttta
    59341 atcgtttcgg gaacttcgtc ccaactcgat tcttcttttt cgctggggcg tgtgaaatag
    59401 gtgtaaacat cgaaattaag gaatgccaaa tcaggcccga attttggcat tggaagcttt
    59461 aaaaaagcgc gataggattt gaggcgaaat tcgagcatcc actccggctc gttttttaac
    59521 cgagaaatag tgcgaacaac ctcttcactt aaaccttttc cggtgtttaa aatcgagaca
    59581 tcttcgtctt taaatccata ttcgtattct ttttggggaa gcttattttt catgtttgtg
    59641 atcgtgatct aaaagctcct taagggcatc ccagccgatt gtcgcgcatt taatgcgggc
    59701 ggcctggcga tgcgtgttga taaaaacgat ggcttcgtcg agaaccgtat catcaaaggg
    59761 ttgctcgtga atcatcttct gatattgttc gattatatag agggcatctt gaacattttt
    59821 gccgattacc aactcggtca taatgtcggt cgaggcggtc gaaatcgtgc aagctatacc
    59881 atcaaaggcg cagtcggaaa tgaccccatt ttcttcaaga agaaaaacat tgatatcatc
    59941 gatgcaattc ggtgaatcga tgtgcatcat ctgatagcgg tcatcatgcg gtgtccgctt
    60001 attccgaggt ttctcataat ggtccataat aattgcccgc atcatctgcg gatcaagcga
    60061 aataggcatc gaggaaatcg cctccttttt ctaatgccga aacaaaggca tcaatatctt
    60121 ctttggtatt gtaaaaataa agagatgcgc gcaccgtcgc aacttcgccc aaatagtcga
    60181 gaagaacttt cgcacagtgc tgtcccgaac ggcaggcaat gccttgcgaa ttcaagtatg
    60241 tcgcctcatc ctgagcaaac acacctttag tattaaaggt gataatgcca gattccgctt
    60301 tttcgttata caaaataact ctttcattct tcttaagttt ttcaattgcg tatgtccgca
    60361 gttcatcttc atagcgacga atattttcca tgccgagtga catcagataa tcagcggcgg
    60421 ccttgagccc atatatgcct tcgatgttct gcgttccggc ttcaaatttt gaggggggtt
    60481 gcagataggt gacatcaccg cacatatcga atttggcatt catgcctcct cccgtcataa
    60541 acggcgccgt cttttcaaga agtccatatt ttccgtagag gacgccgatg ccggtcggac
    60601 cgaccatctt gtggccggaa aaagagagaa aatctacatc cagatctttg acatcggtcg
    60661 gcatgtgcgg caccgattgc gctccatcgc agacgacgat cgcgccatat tgatgagcga
    60721 tttttgtaat ctctttgata tcgacgataa aaccgaggac attcgtcaca tgagcgacgg
    60781 cgataatctt tgtcttcttg ctgatgacct tgcggagatt ttccgggacg agtttgccat
    60841 cttcattaag cggaatatag ttgattttgc acccggtcat ctcgccgact ttaaaccacg
    60901 gtaagacgtt gctggcgtgc tctgcctgag ttatcaaaat ctcatcatct ttggtcagat
    60961 atttcgtccc gtaaccataa gcaattaagt taatcgacat cgaggttccg gacgtgaaga
    61021 caatttctct tttttcggcg ttgataaact ttgctaaagt ggcgcgggcc tcatcaattt
    61081 taacatcgac gttatagcat aaatcatagt cgccgcgatg agagttagcg gtttcatatt
    61141 gaaaatatgc attgaccgct ttttcgactg catatggttt aaaagtcgtc gaagcattgt
    61201 cgagaaaaat gagcggtttt ccttgcatca tgactttatt tttaaacatc ggaaagtcat
    61261 ctcttattaa atacggatcg atttttttca tacgcgttcc tcaataattt tagcaatttt
    61321 ctctttcatc gcctcgtcat ggaagtaatt ggcaatcgga agcagatagc cataagtgat
    61381 gagcttcttg gcttgattta gattcaatcc gcgggacatc agataaaaga gatggtcgtc
    61441 gcttaatttc ccgacgacgg cagcgtggga ggcctcaact tcattttcat cgatgttaag
    61501 gcgtggatcg gctttggcaa ccgccccttc atcaaagacg ataatctttg cattctgttt
    61561 ggtcttgctc tttttcgcgc cgcgggcgat ttcgttagcg ccgctgaagg cgagacgcga
    61621 agaattgcgg gcaactccgt aattgtccat caagccatat gtactctgtg cgagatgatg
    61681 aaagtgaacc gcatatttct tatcatcctg attactggca agagtggaca gacgccatgt
    61741 tcctcgagcg ttgcttccga ctaaattaat cgcgacatca aggcgagaat ttccattgga
    61801 aaagtcggcc atggtcgcaa ataattcagc attttcctcg acttttacaa cgattttacc
    61861 atttcttgat tcgccgaaat taatgagatt aagagctaga ttagcccctt tgcccacgtt
    61921 tatttccaaa ccgtcaaagc cttcgctgaa gtcgacgata agctcttgtt tttggcagtc
    61981 ggcaagaatg attttccgga tgttatttgc catcccgaat cgcctcttta gtggcgcata
    62041 cgccaatcga caccgcgctc atggccggtt cttctttttc gatatcgata cccagttcgg
    62101 ttttaatcca ctcatatcct ttagtatcta ttttttgaat gatttccggt ccgccttcga
    62161 ccgcgatttt tccgtttacc ataacgatgg tgcgatttaa attaatcatg ttgtataagc
    62221 gcgcatagtg tgagacgacc aaaaagccgc gccctttttc ttgttcttcg cgaatggcgc
    62281 tggcgacgat ggtcatcgca tcgatgtcga gcccggaatc aatttcgtca agcaaggcaa
    62341 aacgcggttc caaaagaatc atctgcaaga tctcgttgcg ctttttttcg ccgcccgaaa
    62401 agccgacgtt taacgagcgg ttggccatct caaagggaat gcccatcttt ttatatgtcg
    62461 aatcgagcaa acggtaaaac tgaaataatg atagaggttt agctcgtcgc gcgttgatag
    62521 aggctctgag aaaatcggca ttgacgacac cggacacttc gcttggatac tgcatgccta
    62581 aaaagaggcc ggctttcgat cgctcatcga ccgacatggt taaaacatcc tgatcattga
    62641 gaaggatttg cccgctggtg attcgataac gggggttgcc cattatcgcc tgcaagagag
    62701 ttgacttgcc gtgtccgttg gggccgagca aagcaaccgt ctcaccgctt ctggcagtaa
    62761 ggctcacacc tttgagtatt tctttgtctt ccacgttaac gtggagatcg actattttaa
    62821 gaacatccat gctatcatct cctaaaatct ctcatattgt atattaatca atatacatat
    62881 tcaaacagaa cgataagaac ttgccttttg aaagactggc gataaaagct tttcggattt
    62941 aaaaaatcca aaaaaaggaa agattatttt tt
//
LOCUS       DAOSSL010000002        55720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_638_length_55720_cov_18.468840, whole genome shotgun sequence.
ACCESSION   DAOSSL010000002 DAOSSL010000000
VERSION     DAOSSL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 55720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55720
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_638_length_55720_cov_18.468840"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLH17_00395"
     CDS             <1..1143
                     /locus_tag="PLH17_00395"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOR20273.1"
                     /translation="MNAPLGYTRVDDENDGDGYRLYRNDNHIELGFSFDTLYYKRSCG
                     DGRYNDFHTTSNHSFDVIRNEEAYLKGAILNDEDVLDVVSDHPHFNLRYAPPLEADRL
                     SITRTLYDTDFYFQPRDPDRYLDYETFPFAEADEKVVSDKTQLVLAPSTGDYLTTEPA
                     YIMLKYPLKMPDTDYRGRVYLIGDGIDDEGNPNPTTSRVITTDYHSNNEAYQKYFRGF
                     YANEPVKKIIVVPAGNGIVYQYSDIYVETYDHILNKLANLNQYPLEDVVYDTNYFSFT
                     TDFATERFIVTQVTYGDGWKVFAKGEDGISEQLKTYNAQGGFVGFVSKAGATSYEMKY
                     VTPYFAEGLIVAFSGFVIFGGAIGLGAYLDLKKKSKLEDQDKEKRQ"
     gene            complement(1148..1996)
                     /gene="rsmI"
                     /locus_tag="PLH17_00400"
     CDS             complement(1148..1996)
                     /gene="rsmI"
                     /locus_tag="PLH17_00400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOR20274.1"
                     /translation="MRELNFKGNTPILYIIATPIGNLNEMTPRALDIIKEMDYIACED
                     TRVSGKLLAHFEIQKPLVSCREHNEISGAIRIISDLKNGQKIAYLSDAGYPSISDPGN
                     RLVSEALANGIKVSVISGANAMLNALVGSGLSTERFYFHGFLSPKEKVRQEELRTLLK
                     RRETLIFYEAPHRIVKTISDMLKIFGNRKACLARELTKKHEEFIRMNLTDLSMLDKET
                     LKGEMVLVIEGNNEEVAAHINDDEVRKMISNFIDIGMTPKDAIKQVSDLLKLAKNRVY
                     KIYHQN"
     gene            complement(1993..2823)
                     /gene="ricT"
                     /locus_tag="PLH17_00405"
     CDS             complement(1993..2823)
                     /gene="ricT"
                     /locus_tag="PLH17_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOR20275.1"
                     /translation="MPATINNYFIGVRYYSSSKAYYFGTENNDIKLGDHVVVESVRGL
                     EIAKVVIEPQDIAKYSSGLELKPIERKANETDLYVFEENKKRAAEALKYCLQEIQNLN
                     LDMHPLDAEYTLDGTKITITYVAEERVDFRELLKILAGRLRCRIELRQIGSRDKAKMV
                     GGIGICGLPLCCSTFLHSFDGISISRAKNQMLSLNIPKISGHCGKLICCLLYEDEQYS
                     ELKKDFPAVGERFTIDGLSYRIGSYNVLSRMVRVESSEDVKFLPLEDVKKYMRSKDKK
                     "
     gene            complement(2824..3804)
                     /locus_tag="PLH17_00410"
     CDS             complement(2824..3804)
                     /locus_tag="PLH17_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR20276.1"
                     /translation="MLASEYIKKYQSVLYKVFSNALENNKLTHAYLLSGEPGTPLKDV
                     ALFLAKSLVCDMPEPLACERCLTCIRIEDGSFADIMIYDGAETTIKKNDVSEITSNFE
                     KTALEAKGIMIYILHLVENMTTEAVNSLLKFLEEPGKNVFAFLTSENEAKVLPTIISR
                     TQVLRLKTIDKQQLIEESVGQGCPRQDAEILSFFLNDPTTIINTYQSQEYIDAKNALE
                     KQLEGLRRSREEAIFVSQSEIVPAIKSKEAARFYLDMLTIVFQDLLNIRVNNRITLTS
                     YDSILHELAARMGHLDETLIEIMTSRGKLDLNVNVALLLDHVIIAITKEK"
     gene            complement(3791..4426)
                     /gene="tmk"
                     /locus_tag="PLH17_00415"
     CDS             complement(3791..4426)
                     /gene="tmk"
                     /locus_tag="PLH17_00415"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOR20277.1"
                     /translation="MFITFEGPEGSGKTSAANEVIRILVARGYDVLYTREPGGTPIAE
                     EIRNIILDKKNTALDARAEALLYAASRRQHLIEKVWPALKEGKIVISDRFLDSSLAYQ
                     GAARKIGIENVLNINLFATEGTFPDLTLLFDITPEKGLERIAINKKREVNRLDLEALE
                     FHRQVRETFLELAKKYQERYVVIDASKTLPEVVGVALNVILDRLEQKHARL"
     gene            complement(4439..5035)
                     /gene="recR"
                     /locus_tag="PLH17_00420"
     CDS             complement(4439..5035)
                     /gene="recR"
                     /locus_tag="PLH17_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOR20278.1"
                     /translation="MKDLKSLTRLIDSFARLPSIGRKSAERMALAVLEMDEEDVKEFS
                     SALEGVKTAIHLCPVCGLYTEDEKCEICSDKERDEKTLIVVSSYRDVYAFEKLNTFRG
                     RYHILGGVLSAVAGIGIEDLNIDSLLERIKDDNVQEIILATNPTIEGETTALFIAKLL
                     EGKGVEITRLAYGLPMGGHVDYADSLTLNKALEGRRKL"
     gene            complement(5035..5340)
                     /locus_tag="PLH17_00425"
     CDS             complement(5035..5340)
                     /locus_tag="PLH17_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOR20279.1"
                     /translation="MNMQNLLAQAQKMQRELKKAHEDLAQEEFSVSKGGAVTVVFYGD
                     RKIKKVDIDAAALEADNKEMIEEMLKLAIDEALKNIKLAEDEINTRITGRAEGMMGF"
     gene            complement(5337..7151)
                     /gene="dnaX"
                     /locus_tag="PLH17_00430"
     CDS             complement(5337..7151)
                     /gene="dnaX"
                     /locus_tag="PLH17_00430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOR20280.1"
                     /translation="MAYKALYRTYRPKTFLEVAGQKHIVRTIENALANNKIAHAYLFS
                     GPRGTGKTSMAKLFAKALNCEEGFGHQCNQCGNCLSVNDGSHPDVIEIDAASNNGVDE
                     VRDLIEKVKYLPIKGKYKVYIIDEVHMMTSGAFNALLKTLEEPPAHVVFILATTEPHK
                     VLPTIVSRCQRYDFTKVEDDAIREKMVEILDLEKITYDIHAINAIIELADGGVRDALS
                     ILEHVLAYAGNSIKEKDVFDLFGLASNKEKLDLIRSISKGDVKDVLKRMESFIASGID
                     IKRLTSNLLDILKDALIYSRTEEHILLSVITEKDALSLKELIDIDRLNEMIEILLKAQ
                     NDFKVVSNIRSLFELVLLRLATSSKKATIPSEDNQTPEMRKAEPQQIIKPTEKKEEVL
                     KKAEVIAPQTVIPEREINAQQPTPIEAAPLIETNGSTVPSWLLEDEMAIDEKNRPAEE
                     VKTKGKDSPGQCTIDLAGDKNALDDETMIKIMVLGDKEERQNISKKWDDFIGLVAHPR
                     YGLVASLLKDGRPYIVAKDVLVLEYDFDCLAERINIVTNQSSIGELMKNIIGRDVFVY
                     AIVRSEALRLQKSYFNLRQLNRLPKAKDIQLNLKGATI"
     gene            7298..8371
                     /gene="prfA"
                     /locus_tag="PLH17_00435"
     CDS             7298..8371
                     /gene="prfA"
                     /locus_tag="PLH17_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOR20281.1"
                     /translation="MEDSMLKRLDATKKRLAEIDEELADESVMRDISHFKNLSKERSN
                     IADICEKYDEYSKYAAEREEALAMMSDSDSELAELGKQEFHSLNQKLEDIEQELHLML
                     VPKDPNDDKNIIVEIRGAVGGDEANIFAGDLFRMYTRYAERNNWKIQMIDENSSGTGG
                     FSLVSFMIKGDGVYSKLKFESGAHRVQRIPRTEASGRIHTSTSTVLVIPEADEIEVVI
                     NPGDLQVDTYRSQGAGGQNVNKTESAVRITHLPSGIVVSCQTEKSQIQNREIAMRMLR
                     AKILSDLQAAQEEKIGNERKLKVGTGERSEKIRTYNYPQNRVTDHRISLSVQKLDRIM
                     EGDLDEILDALVHYDQQQKMLGE"
     gene            8373..9218
                     /gene="prmC"
                     /locus_tag="PLH17_00440"
     CDS             8373..9218
                     /gene="prmC"
                     /locus_tag="PLH17_00440"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOR20282.1"
                     /translation="MPTLFELYQNIIRESPFAQANEVALRALLCEIQGLIEMSDFYLK
                     KDEEIKDLPTFLSYLARFLNGEPYQYIIQKARFINRDFFVDNRVLIPRMETEEVVYKA
                     IELIRDSNNSSSPSIADLGSGSGIIAITLKKTFPHSQVFASDVSKDAIEVAKINAEKF
                     GADISFFRGDVLEPFIFSGIKLDFLVSNPPYITNIQEIDSGVIKYEPYSALVDKDELG
                     FYKKVFSMYKAVAKYPFTMIFEIGHDMKDKITTLLGTMIPLAKWEIIKDINGKERILS
                     IYIEK"
     gene            9232..9837
                     /locus_tag="PLH17_00445"
     CDS             9232..9837
                     /locus_tag="PLH17_00445"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOR20283.1"
                     /translation="MKTISISDINLASQLLKEEYIVAFPTETVFGLGIIYDSERAFEH
                     LAEVKRRRPDKPFTLMCADISDIDNYAVINERAKRLIAHFLPGELTIVLNIKPGLPQW
                     VDFGTGHIGIRVPDDEIVRKMIRLVGKPLLVPSANRADQRPLTTSDEVISEFGEEIGG
                     VVLGRSKGATPSTVIAAYDKITMLREGKISLADITKVLGGD"
     gene            9839..10282
                     /gene="rpiB"
                     /locus_tag="PLH17_00450"
     CDS             9839..10282
                     /gene="rpiB"
                     /locus_tag="PLH17_00450"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOR20284.1"
                     /translation="MRIVVASDHGGFEYKNEIRRHLEKRGFQVLDVGTFSNESVNYPD
                     YAAAAAKKVANGDADRGILVCTSGEGVMIAANKVKGVRAGIGYNDTVSRLLREHNDAN
                     VISFGQKFMELDDVIRRVDIFLETPFNGGRHANRVEIIKNIEKDK"
     gene            10355..11503
                     /locus_tag="PLH17_00455"
     CDS             10355..11503
                     /locus_tag="PLH17_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOR20285.1"
                     /translation="MFKCSRCGNKDPRYIGYKNEQPYCRRCIIFSGSGAKENDGKTYQ
                     SKLELHYELSSEQTEASNRILNAIHNGRDVLINAVCGAGKTELVFAVIKEALIKGRQV
                     GFAIPRRDVVIEIHKRLKTVFPRNKIIAVYGGNNEELQGEVVVLTSHQLYRYESYFDL
                     LIMDETDAFPFHGNPVLEALFKRSCRGNYVMMSATLTETQIKKFASSGGEIISLDRRY
                     HGYPLPVPDVILLLGILKHFFLIRKLRSFLAENKPVFVFAPTIDKCEDLSVFVTKWVR
                     GGTYVHSKRLDRQEVIKDFRKGKYKYLVTTAVLERGVTVKNLQVVVFECDHEIYSASS
                     LIQIAGRVGRKKDAPQGEVIFLSDRKTRAMEEAIYHIRAKNKSMPNVL"
     gene            11493..12095
                     /locus_tag="PLH17_00460"
     CDS             11493..12095
                     /locus_tag="PLH17_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOR20286.1"
                     /translation="MCFEKIKEDTFHSLVHADLCICQKCQDQFVAIFENKIINDVKVC
                     ALYDYNEFIQSNLFKLKGCGDIELAPCFLNYHLPWLMARYRGHIIVPAPSYGDRDAKR
                     GFNQVEEIFRSSKLPLIKAIIKTEDRKQSDLSQDERNKIKEIIAWKEQADISGKKVLL
                     VDDVMTTGSTIRACIDLIKHHHPKSLDVLVISRVANSEQR"
     gene            12163..14580
                     /gene="secA"
                     /locus_tag="PLH17_00465"
     CDS             12163..14580
                     /gene="secA"
                     /locus_tag="PLH17_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOR20287.1"
                     /translation="MANFIEKMFRFDQRILKKYFRESEKVLAYEQEMAAKSDDDLRAK
                     TDEFKLRLKNGETTQSIKYEAFAVAREAARRTLGQFPFQVQVIGALVLDQGDVAEMKT
                     GEGKTLTATMAVYLNALKGSGVHVVTVNEYLAQRDADWMGQIYRFLGLTVGVNIREKT
                     NSEKKEAHNCDITYTTNSELGFDYLRDNMAQNANSRVLRGLNYAVIDEADSILIDESR
                     TPLIISGGAKASASQYTVADRFVKTLKKERDFEVDIKTKTCSLTEAGNTRAERMFGIR
                     NLYDPEHQDLVHRIVQALKANYIMTRDVEYLVDGENQIQLIDQFTGRILKGREYSDGL
                     QQAIQAKENVEIKQETITLATITYQNFFRLFKKLSGMTGTAKTEEEEFRKIYNMRVVA
                     IPTNRPVVRDDATDYIYAHKPAKLEALVQEAKERHEKGQPILIGTVSVESSEEVSRLL
                     TAAGLKHEVLNAKNHAREAEIIALAGNKGAITLATNMAGRGTDIKINDEVRALGGLCV
                     LGTERHEARRIDNQLRGRSGRQGDPGFSRFYVSFDDELMRRFGAENVQKRIESLGTEA
                     LESRLFSNFITSAQKQIEGKNFDIRKNLLDYDDVIAKQRTAIYEKRDSILFADNINDL
                     IYDFFQTAGLFLAKKAISSESRDGTISGESLRKIVEPRFMAEGTFRANAYDEAPADEA
                     GQDLGDTLYDRYVNRRKEWGEEIANQVERQIALQTIDQNWTRHIDTMSRLREGIYLRS
                     YANTNPLQDYVNEGHALFRDMLDKVSADVCLKLLNAIIQIKKPEETSVPDDKVIDTPA
                     EPVREKE"
     gene            14635..15702
                     /gene="prfB"
                     /locus_tag="PLH17_00470"
     CDS             14635..15702
                     /gene="prfB"
                     /locus_tag="PLH17_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOR20288.1"
                     /translation="MGSVILFDVASLEVKQKELEAIQNESGFWNDQKRAVSVVSEYNK
                     IKDIHDAYIKITKSHSDLDELIKVTSSTDEDMILLIEEMLNELSQDVKNLRSELYFTG
                     KFDRLNAIVTIHPGAGGTESHDWADMLYRMYVRWAEKRRYKMQVIDYQAGEEAGIKTV
                     TLAIRGKNIYGQLKGEKGVHRLVRISPFDSNARRHTSFASVNVVPEFDNEINIEINEN
                     DLKIDTYRSGGAGGQNVNKTESAVRITHLPSGIVVSCQVERSQISNREMAMNMLKGQL
                     YELEQSRQTSELKSIVGEQKAIEWGSQIRSYVFCPYTLVKDNRTNYSEGDVHAVMDGN
                     IDGFINAMLDMEARNKKDILS"
     gene            15745..17724
                     /gene="uvrB"
                     /locus_tag="PLH17_00475"
     CDS             15745..17724
                     /gene="uvrB"
                     /locus_tag="PLH17_00475"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOR20289.1"
                     /translation="MDTHKFNLISSYKPTGDQPLAIKQLVAGLAEGRPIQVLLGATGT
                     GKTFTASNVIQAVQRPTLVLVHNKTLAGQLYGEFKELFPENRVEYFVSNFDFYQPEAY
                     LPKSDTYIDKNAVMNEEIEMLRTSAINSVLERRDTIVVASVASIYGLTDPDEYRNLVF
                     EIRVGETIDRKALFHKLIDSQYQRNNMDLVPGSFRVQGDIIQIVPPHTAEYYIKIDTF
                     GDEIERIAMVDILTGEVKRTYLTYPIFPAYGHASTRQRIKEATVTILAELEERLAYFR
                     KEGKLLEAERLEMRTRQDVESMLEFGMCPGIENYSRHIDKRQPGERPFTLIDYFPKDF
                     MLIIDESHVTLPQVKGMYNGDRSRKLTLVEYGFRLPSALDNRPLNFSEFEAIMPQTIC
                     TSATPGDYELERANREVVEQIIRPTGLVDPRIEVRRTLGQIDDLIYEIKQRILAKERT
                     MVVTLTIRMAEDLTAYLKERGIKVAYLHNETKTLERTEIIYQLRKGKYDVLVGINLLR
                     EGLDIPEVSLIAILDADKEGFLRSSRSLIQVIGRAARNKHGLVIMYADRMTDSMRFAI
                     AETDRRRSIQASYNEENGIVPETIIKDIRPPLKNMEDEEEVLKVFTAKASRSEIEARI
                     KELEKQMREAAKGYDFEKAAEIRDIIFEIKATLNK"
     gene            17793..18029
                     /gene="secG"
                     /locus_tag="PLH17_00480"
     CDS             17793..18029
                     /gene="secG"
                     /locus_tag="PLH17_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOR20290.1"
                     /translation="MQWYDVIFLIVALIIIVLSLLQGGKSEGASGAITGGGTNIFVKT
                     KERGSELVITWLTFGFAIVFFFFAILISVLQNVA"
     gene            18114..19643
                     /gene="cls"
                     /locus_tag="PLH17_00485"
     CDS             18114..19643
                     /gene="cls"
                     /locus_tag="PLH17_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOR20291.1"
                     /translation="MKNFLKMHLWRLLIIFFLFLVQISIIVLGILYIRDYYPYVYLGF
                     LVVSIIIVLSIIANRSNPSYKIAWLALILIFPVFGGFMYLMFGNKKMNRRSKKMVSKM
                     NAMIEAATKKTESVLDDLARDHPDAHKQSTYIKNTTGFGPYQNCYLEYYRSGESFLAT
                     LKREIKKAKKFIFIEYFILAQGQVWDSLLKLLVQKAKEGVDVRVIYDDMGSISTVPHN
                     YDRKLQKMGIKAFAFNRFLPFLNARINNRDHRKMLVIDGSTAFTGGINLADEYFNLKS
                     RFGYWKDNVLLIRGDAVRSMTLMFLTFWSVLSGKIEEFEPYLAKSKELKIVDTGYVQP
                     YADTPLDDEAVGENVYLNMMGRAKKRIYITTPYLILDNEMVIAFSNAAKQGIDVRIVT
                     PGIPDKKMVFQVTRSYFRRLIEAGVKIYEYIPGFIHQKVFLADDDFLTIGTINLDFRS
                     LYLHFENGVWLYKSAIIQDVSADFDEILADSRLVTLADTKSGPFHRLWISFLRSFATL
                     L"
     gene            19714..20505
                     /locus_tag="PLH17_00490"
     CDS             19714..20505
                     /locus_tag="PLH17_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOR20292.1"
                     /translation="MEIKEYVAMRKQQIRDEVASMKVRPHLAIVQVNEDEGSNSYIRG
                     KLKDAEELGVLATHIKLDVNTSEAEVLKVVESLNQDSGVHGFIVQMPLPKHIDENKVK
                     VAIDPRKDVDGFHPLSSLKACTPRGILDYLKFNKVELLGKNAVVIGRSNIVGKPMASL
                     LTKESANVTVLHSKTSPQDMKFYLEHADIIVVAIGKKWLIDGYLFKPDAIIVDVGINR
                     VDGVLYGDVKPGQKVRLQTPVPGGVGPLTRLALYENLMEIMKHGI"
     gene            20495..21085
                     /locus_tag="PLH17_00495"
     CDS             20495..21085
                     /locus_tag="PLH17_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20293.1"
                     /translation="MEFKITNTQVYGLERAIKASGNPMRVEIDTSEINDKDFVRATKL
                     GIAKSGEGHDNFLKGILVQMDVTAPLFWWKQAQRYHWFDFISSQSTMHCLLKFDVTKQ
                     CVSDTNEEVVRIVSEMIKEYLSLSEDDNRKKTLWREIVASLPSGFCLGATMTTNYQQL
                     KTMYMQRRYHKLNEWRVFVEWCDTLPHFKELTGVSR"
     gene            complement(21129..21797)
                     /locus_tag="PLH17_00500"
     CDS             complement(21129..21797)
                     /locus_tag="PLH17_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20294.1"
                     /translation="MKKMYKVWAIWQICEAVILLAAGVLTIIFSDDPDLYKWIFLAIG
                     AFIILDGVLRVLMPFFNKDPGDNSLFTGIFEVTFGIVIVLRSAAITEILMIFIATLLL
                     VISPVAIADGIIRIKKKQETLFFPVLEFIAALLFITIGVIILVSIQNAQNVVLIVIGA
                     ILAILAIVEIAFTIKTLMKVKERDKKAQKKAEAKTKGKVVKKEVVEPVIVEEGEAFPV
                     DKVK"
     gene            21889..23118
                     /locus_tag="PLH17_00505"
     CDS             21889..23118
                     /locus_tag="PLH17_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOR20295.1"
                     /translation="MEVQVLRKYAELIVKVGANVQKGQDVIVYAELDQPRFVEFVVSE
                     AYKAGAKSVTVRWSHNPVDKIRYKKESLKSLSEVPNWIIEREKHYVKTLPATIYLVSD
                     DPDAYKGVNQAKLAKVGQAVYPILKPFRDERENKYQWTIAGLPGPAWAKKVFPNLPKK
                     KAMEKLWGAILASSRADHGDPVANWRAHNADLINRCAFLNSLHIKSLHYKSKNGTDIK
                     IGMLPNVNWLGGGDKTLGSDIFFNPNIPTEECFTTPERGKAEGIVYSTAPLSYQGELI
                     ENFHIVFKDGKAVEVHAEKNEALLKQMVSMDEFAGYLGEVALVPFDSPIRNSGILFWN
                     TLYDENASCHFALGMGFTNTVQDYGKYSKEELTKMGINDSMIHVDFMIGSNDLSIVAT
                     TQDDKKVQIFKNGNWAF"
     gene            complement(23182..24453)
                     /locus_tag="PLH17_00510"
     CDS             complement(23182..24453)
                     /locus_tag="PLH17_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20296.1"
                     /translation="MKRSKKEILKKLRQEGSIITPDILNNVYKAIGVNPITISEKERI
                     IEQRLKSEAEVFVPQKKIAVSATEEKPRLSLKALFQRPRFVSAFATAMVAVILSVTLI
                     GLRIGGFFTTDPTDTSTGTDTGSHPVTVPQPIQNDQQVFSVGAVTANVLFDFVEDSGT
                     SGDFRALVNDTDVDVVIGQFPPYLEMIEQLISSDGELETISDISEREGYDYKETVIAY
                     DLLGSHLNYAIYYNVETLIEDGAESTYYFSGIIIYGDDTNEYVIEGRRIVEEDETKVV
                     MTIHYDEINYLESTYKSEEDGYKYNFKIFENDNLLNNTKLKIETSEGKTKLKLDFIDT
                     DEKNSYSYDISIEDDTPDMISVRYHIKLDKEIYNGTIEIQIVTNSETGEYEYEMVIRP
                     NGQGQGTTHTEGRGNNHGGNGNGGGNGGGGH"
     gene            complement(24440..24979)
                     /locus_tag="PLH17_00515"
     CDS             complement(24440..24979)
                     /locus_tag="PLH17_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOR20297.1"
                     /translation="MASELERAMICLQNGDVKCLDAIYRLTSKGIFTFVLPIVKAYDV
                     AEDVMQNTYIRAYENISSYTPGTNPRNWLLTIAKNLALGEVKKRSREISYDFNDENVE
                     TQGVYDLNPNLDTPTIDLANRVLNEGEMNIVLLYAIGGYKHREIAEMLNLPLGTVTSK
                     YNAALKKIRIAYEKENEKK"
     gene            complement(25146..25457)
                     /locus_tag="PLH17_00520"
     CDS             complement(25146..25457)
                     /locus_tag="PLH17_00520"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HOR20298.1"
                     /translation="MSGRGMKKWRPFSSLEEQKPSLQQMKYQKGKCPKPQISRDQAEK
                     IDGYLHDYKGKCFQITFYEDGYVLTLEEAIQKVSCESCTIKTESRTISFNNLINLEIL
                     K"
     gene            complement(25454..26698)
                     /locus_tag="PLH17_00525"
     CDS             complement(25454..26698)
                     /locus_tag="PLH17_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HOR20299.1"
                     /translation="MNPVIAAIDLKAFYSFVECLDRKLDPFATPLVVCDESRGPGTIV
                     LSVSPFLKALGVPSRLRKRDLPKRDDIIFAVPRMARYIEMSAKVVSIFLDFVGEDDLH
                     VYSIDESFLNIGPYLKLYKSTPRQLVRKILDKIKKDTGLFATAGISENLFLAKSALEF
                     EGKKAKDGIGEWTEDDIKTKLWPVSPLSEMWGISSRLEKRLNEIGIVTIGELANAPEA
                     LLIKYFGAIGRDLREHANGIDDSDIREKYIPKETSLSNGQSLFRDYAKNEMPTIIREM
                     SDDLALRLRLEGKKTGLVALGIGYSYSFGGGFSRQLTLVKSTSENDLIFDGLITLFRK
                     FCEDKPIRRVDISFGKLSFDDFEQLDLFDEKNKQTNKRELRKALDEIVLRFGRDAVLR
                     GSALLEESNAIKRHAQIGGHRK"
     gene            26876..27760
                     /locus_tag="PLH17_00530"
     CDS             26876..27760
                     /locus_tag="PLH17_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20300.1"
                     /translation="MNGEQIAIIIVNILSFPFFIDGIIILMIILSFLLGLAKKGWHSL
                     WRFVFVIFLLAGAALFAVKPLATWLASDAFFTLIGYQPVVDYGGSHTINITSFTELIV
                     VMGRLSTDRAKFTAAYSAELAFNIAKAVAWFAIVLVVHTISWVVSALLWPLVRLIIPA
                     RIRKKKMRVLGGLIGIAQTLVIVASYMFATSAISPGFTYLYSAGNYGAFGINDYLVGI
                     TAGLNRENSWVFSWLALDAKVFQYKIGETHYFVNLEFFYLSESLINDDLTDEEKEAII
                     SAFEEECFNEEGEFVCTV"
     gene            27809..27884
                     /locus_tag="PLH17_00535"
     tRNA            27809..27884
                     /locus_tag="PLH17_00535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27842..27844,aa:Ala,seq:cgc)
     gene            28045..28326
                     /locus_tag="PLH17_00540"
     CDS             28045..28326
                     /locus_tag="PLH17_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR20301.1"
                     /translation="MTGANAGIGKACSKQLAQLGHRVIMACRNQQRGEAARQEIISQT
                     GNHKVMLKIVDLSSLSSLKKFVAEIAQEYERLDVLINNGADFDISRRKK"
     gene            28326..28397
                     /locus_tag="PLH17_00545"
                     /pseudo
     CDS             28326..28397
                     /locus_tag="PLH17_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28404..28691
                     /locus_tag="PLH17_00550"
     CDS             28404..28691
                     /locus_tag="PLH17_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20302.1"
                     /translation="MLEKSSAGKIINISSQGLVMYPFIKLNISDFNCDKKYNPATQYY
                     QNKLALLMLSLYMKENFAGKTKIVAIRVTNVKVDVNRYPNLTDFQKKALQF"
     gene            28720..28890
                     /locus_tag="PLH17_00555"
     CDS             28720..28890
                     /locus_tag="PLH17_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20303.1"
                     /translation="MAKAYVSMAEDDSFTGFYVDEKLREVKVNRGATDHKRQILLVDY
                     LQKIAGLAVAKS"
     gene            29018..29587
                     /locus_tag="PLH17_00560"
     CDS             29018..29587
                     /locus_tag="PLH17_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR20304.1"
                     /translation="MRKIILIVSGALAILAIVIGGSWISAYNGAIVREAEVVEARGNV
                     HAALEARYVKVDVLIGAIEDANATIEGYLTIIADAREAFAAAIENGNAAAADEAAALA
                     DGTFVTLVAYMEENPDSYNTVSLYSGFISEFNASTNVVTFMIIEYNGTVTDYNTHIKT
                     FPNIIFVGGRAVYNSYELTNYNLELPTFK"
     gene            29599..30414
                     /locus_tag="PLH17_00565"
     CDS             29599..30414
                     /locus_tag="PLH17_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20305.1"
                     /translation="MTNRQTTARKWFFKGLAFFLIAFLTACQGNPKPHIRPTEEFYIN
                     DRSNVLLNSTRWTIYAYGEELYEDSQAQEYKDQEISGAQFVVATYVGQPDDFYPPDLF
                     NSWGPGENDMGLLLILYFQQGAEEYEFDYQVIDYVFGARMMGFISAFRMDELISEYFN
                     DPEISPSDYDQRLISLYFGIMQELYLSVYDYDSYNYQSFMDEYALNKYEEFDLLPSDY
                     ERDPLPAWAWVLIAVGILLLGVFPGRYILPYVFSAFTGGKGGGGRTIGYWFRR"
     gene            30441..31538
                     /locus_tag="PLH17_00570"
     CDS             30441..31538
                     /locus_tag="PLH17_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20306.1"
                     /translation="MKINLPPDSLPKLKKLISVKDENILVSELLLRYLLNSEKYPLKA
                     EFKRRSPNASTKEQLYAAMLAEKISPKHRGKQDVLLRHLNGRCALLDGDYYRDNPFFK
                     TIKISEWSIGKWRLAFDRYAPYQAFAFRDIDVSSQGYEEKTHLGFFEEPFSFPVIMQE
                     DRVWMSVTPHEIETMRQAVIEARGVVAVMGLGLGYYPYMIAQKDAVKKVIVFEKDTEV
                     ISLFTQHLLSQFPNREKIIIIEADAVKDFAKCVQQTYPDYVFTDVWQGVDDGLPLFVE
                     MKKEEKHFPGARFRYWIEKSLLAMVRRIIITIFEEAIEGFTSEDYENADTPLDVIFRD
                     MFNKLGNAKFSSFDDILNVLTDEGISALLSR"
     gene            31625..32845
                     /locus_tag="PLH17_00575"
     CDS             31625..32845
                     /locus_tag="PLH17_00575"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009169432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOR20307.1"
                     /translation="MKSIRELYRIGQGPSSSHTMGPQKAAAFIRDKYPRATSYDVVLY
                     GSLAFTGRGHLTDKAIINTLAPRPCIVTFDYKSKVAHANTFDITIHQPDQTDIKHRIL
                     SLGGGAISIDGETFFEVGEKYAEKTFDEIKKLCLANNWRFYDYVADREGPEIWNFLAK
                     VWKQMKLTIENGLNNRGFLPGSLKIYRRAGDLYNVFDENETLAARSYRMMSAYAFATG
                     EENASGGIVVTAPTCGSSGTIPALLKYMAEQYHHSDQEVLEALATAALIGNVIKHNAS
                     ISGAEAGCQAEIGTACSMAAAAYAELLKLDLNQIESAAEIALEHNLGLTCDPIGGYVQ
                     IPCIERNAVAASKAITATLLAKYIAGTHAISFDSVVATMLKTGKDMRKAYRETAKGGL
                     ANLYKNLKKSRAKR"
     gene            32889..34055
                     /locus_tag="PLH17_00580"
     CDS             32889..34055
                     /locus_tag="PLH17_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOR20308.1"
                     /translation="MKIAIIGAGASGLVAAIKCKEKFPEYEVFLFDRMDAVARKVKAT
                     GNGKCNIGNLDSSFERYLHSSFVSRILKDYDQETTLLSLGIPTKTMYGSGLYPVSESA
                     SNVVTLLEERARLLGVNIILNTRLSEYCLGDGKINLFFDKYQAKFDKVIFSVGGKSNP
                     NLGSDGSLFEVFRRHSYLVADLTPGLCPIRVKEDVKALFGQRFHTMASLFIDGQFVHE
                     EYGEVMFKKDGLSGIVIMNLSSMIAHKKTNDAKIYLAALNQKNEIVTAKNLFEISKKN
                     HNPLLAYVGEELAAYICKTADISADHELSYEECQKLANVAAAIPFTYLDRYDFSFSQV
                     TIGGIAIENLSADLSSKVEDGVYFIGEVIDVDGPCGGYNLRWAIGSALHLVNHF"
     gene            complement(34114..34413)
                     /locus_tag="PLH17_00585"
     CDS             complement(34114..34413)
                     /locus_tag="PLH17_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR20309.1"
                     /translation="MCDISIAYDEDLERVEKVILNNIEELKSKIPDIVEGPFYRGVQS
                     LSDSSVVIRMYARTDELKKYQVTRDLNKEMKLLFDRNGIQIPFNQIVVHYEDKKE"
     gene            34545..34706
                     /locus_tag="PLH17_00590"
     CDS             34545..34706
                     /locus_tag="PLH17_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20310.1"
                     /translation="MRLNNIADPELLFKDDKQSGDDVFDEALSAKTERQTEDSGTGEE
                     GWRLNTPGA"
     gene            complement(34703..35071)
                     /locus_tag="PLH17_00595"
     CDS             complement(34703..35071)
                     /locus_tag="PLH17_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20311.1"
                     /translation="MASIKKIRENEKRRRLIAKMLASFAVTATVVVTVVPALSTTPFG
                     VFVNNTVGKFFNVANLFVNNGMALLETAVIIFFVWALNEIVKIVIDLLIRRFSKKSTV
                     GLSFAASSNMEASSSASFWS"
     gene            complement(35159..35575)
                     /gene="msrB"
                     /locus_tag="PLH17_00600"
     CDS             complement(35159..35575)
                     /gene="msrB"
                     /locus_tag="PLH17_00600"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOR20312.1"
                     /translation="MKKIPELSPFQKKVIFENATEPPFDNEYWDNHREGIYVDAVDGT
                     PLFASYDKFDSGTGWPSFTRPLQDGLLVNCDDFSLPRHRIEVRTKKSNIHLGHVFTDG
                     PKKEGGLRYCINSASLRFIPKEELEKEGYQALWKKK"
     gene            complement(35678..37204)
                     /locus_tag="PLH17_00605"
     CDS             complement(35678..37204)
                     /locus_tag="PLH17_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20313.1"
                     /translation="MKLNVKRTFLVGLAFLLISMFWQIYDGVMSIVLVNNFGLNQTWS
                     GILLALDNLLALALLPLFGAWSDKTSTKMGKRKPYVLIGTIVAAIVFVSLAVVDFYQF
                     QAVRAEEISPMLSILNGNGDVIGYYFTVGGNIKDIILLEGNSYGFVYITQVADKVAEL
                     RAAYIFAEITQPNWIYLASFIVILFIVLIAMCSFRTPAVSLMPDVTIKPLRSKANAII
                     NAMGTIGGGISLVLIMVVGTVKVSNYDTFNYLYLFGIVAGLMLAFLAIFMFLVKEVKW
                     TDEMRRESLRLGLETEEDEKVAKGTKGEKMEPAVRRSFILILLSVFLWFFAYNAASSK
                     LSLYAQNILQIENYTFPAMIGFGAALIAFYPIGILSTKFGRRNMILVGIGVLILGFIL
                     AFIASIDLSFGWVIYAAMVVVGFGWAAINVNSYPMVVEMSKGPNIGVFTGYYYAASML
                     AQALTPVISGLLMDAFGMTLTLFPYAIFFSIAAAVTMFFVKHGESKRLETKEIQPKTE
                     "
     gene            complement(37289..38020)
                     /locus_tag="PLH17_00610"
     CDS             complement(37289..38020)
                     /locus_tag="PLH17_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOR20314.1"
                     /translation="MHDLTNFLKIPLAHRGLHDNNYDENSLGAFKAAVESGYGIELDV
                     HLMKDGTLAVVHDDDLRRVAGLPRRISEMTKEDLSKTSLLLSKEKIPTLNQVLEVVDG
                     RVPLLIEMKVDGPFNPELPASILALLKDYPQTNNIAIESFNPYAIRWLSQNHPKLYPY
                     GQLISLSLEGLDKFAAWMFRSLNIRLISKPAFIAFDINCVPYQKLRRLRRRGMPVITW
                     TVDSEQKRELALQESSNYIFESIRP"
     gene            complement(38068..38562)
                     /gene="coaD"
                     /locus_tag="PLH17_00615"
     CDS             complement(38068..38562)
                     /gene="coaD"
                     /locus_tag="PLH17_00615"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR20315.1"
                     /translation="MRQAVYPGSFDPVSNGHLDIIRRAAKIFARLYVLVSVNPNKQYL
                     FTAEERVDMLKAATADIANVVIEASEELVVSYAQKKGNASIIRGVRNTNDYQAEITLY
                     QFNHTIDPEIETLVMFPSHNNLFVTSSAIKELALFGNDISPYVPKEISEFILHTIKHR
                     LKKS"
     gene            complement(38559..39452)
                     /locus_tag="PLH17_00620"
     CDS             complement(38559..39452)
                     /locus_tag="PLH17_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR20316.1"
                     /translation="MERFVKKHLTLKNGEQYAYIEQGQGEKVFLLIHGNMSSSAYFYE
                     LLKRIPHDIRVLAPDLRGFGDSSYNARFDSLDELADDVIDFLSILGVHKVDVLGWSTG
                     AGVAYKLAAKKPSLVKKIISLSGASYRGYPIFKKGDHNMPLIGQIYSSKEEMAKDPVQ
                     VAPALYATATQNKAFYDYIYSLTIYNVKKPEPELLSYLLDETLKQRNLIDIDWSLANL
                     NMSNFSNLYNGGTNDIAQVACPVLLLWGDKDLVVLEYMVDETLAALKNATKKVYSGVS
                     HTAITDIPDQFAADVLQFFKG"
     gene            complement(39463..40281)
                     /locus_tag="PLH17_00625"
     CDS             complement(39463..40281)
                     /locus_tag="PLH17_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR20317.1"
                     /translation="MPFFTFHNKQIFYEDDGSGEPILFLNGIMMSTVSWKTFVPALSK
                     GNRFLRIDLLDMGQSARMDEHYTQDIQVEVVKAFLDHLQIAKINIMGISYGGEVAIKF
                     ALKYQNLLRRLMLFNTTSRTNEWLRDIGRGWNKVGATLDGEAYYNMTIPIIYSSNFYV
                     ANYGWMEHRRKLLIPLFSTPEFQERMKRLVTSAESLDEAANIHRIEVPTLIVSSEFDQ
                     LTPSSEQAYLAEHIKNSTHIIVPKAGHAFMYEKPHIFVSLVLGFTNSSTDKYFI"
     gene            complement(40283..41602)
                     /locus_tag="PLH17_00630"
     CDS             complement(40283..41602)
                     /locus_tag="PLH17_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOR20318.1"
                     /translation="MKHDLSKKIITVEEALKLVKSNDHIFTGLGASEGREFLSKLHTL
                     HGLVKNVTVTNCLPMQDYEFYMNPAYKDTFKLAGWFYNAGMRKAHANGNVSFIPNVLH
                     YAGTKRLDHIKPNIYVGIASPQDEHGYVSLSLGNTYEKQVIKEADLVILEINPNAPRT
                     FGDLEVHVSDVDYFIEVNYPIPEIPDTEPNEKDLLIGKYISELINDGDCIQLGIGGIP
                     NAVAKSLYGKKDLGVHTEMLTNEMAKLAKAGVITGKHKQLFPGKMVATFAMGSRDLYN
                     FIDNNPAVMLLAGSYVNDPYVIANNDNQVSINTTLEVDVTGQCCSESIGSKQFSGTGG
                     QSDTAIGAQNSKNGRSIIALYSTANVRNPETGEREETSKIVCQLKPGAAVSLSRNDLH
                     YLCTEYGIVNLRGTTIEERVEKIISIAHPKFRDSLRQEAIALGIIGA"
     gene            complement(41690..43036)
                     /locus_tag="PLH17_00635"
     CDS             complement(41690..43036)
                     /locus_tag="PLH17_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR20319.1"
                     /translation="MKKINLFVLSAVSAVILAACNPAVEVVKQYDGTEVQGVTAEKIL
                     IGNTAATSGAFATVGVPFNAGINAVLKNFNDAGGFDGATVELVHYDDSFDGAIGLAAT
                     QKLVEQDKVFALVGHFGTNTVAATVDYIKDQGIPMVYAATGISDLYQEAAEGFNKAVM
                     PVQPIYNSEGRMMLARALADDAVSGLNATKIGVISTTDDAGEGMLYGVQKQAELLSVA
                     AKENLVYVTTPAEQGTNHSAPVNTLKTAGCDVVIVAANQVPFTEILNYMRDANFNTKV
                     ITSYVTANTDILRAAAAAGSITADRPVFTNSWLDVTDLAYYDATLNPYALTPEYVAYL
                     TEMNTGGQVAYSGSSYAIAGYVAAKIFLEGLARVKAADKELTWLNYINAMEEEPVDLP
                     MGSEIDYADGKRLGVTDLGFSKLYLADPVNTSALAEVDTLRTLDYVWALVPAAKKA"
     gene            complement(43312..44094)
                     /locus_tag="PLH17_00640"
     CDS             complement(43312..44094)
                     /locus_tag="PLH17_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20320.1"
                     /translation="MKLLEIRNLVVRYGVIEALHGINIDVEQGAMVALLGANGAGKST
                     ILKAVSGLVKPVSGSTIMLNGANVAGKRAHAVSKMGLSLSPEGRQIFTGLTVEENLLA
                     GAYGLKRHTQLIEDEHGIKRKVSISVKAQLKENFDRVYKLFPVLAERKRQQASTLSGG
                     EQQMLAVARALMNSPKILVLDEPSLGLAPLIIQDIFNTLVEIKKQGTTILIVEQNALA
                     TLKIADFAYVLELGNVSISGKASELINDQRLIDAYLGGGEKK"
     gene            complement(44091..45584)
                     /locus_tag="PLH17_00645"
     CDS             complement(44091..45584)
                     /locus_tag="PLH17_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20321.1"
                     /translation="MALKNKVMAYLLGKYVDFLTQRVTFYKSRTSLYADFEVEHLKDI
                     DDAANRKIELRIAKNARLYATADYEASDKKEVFEGTSKLEIAAYQKKLRMRYRIEREK
                     ARRAIAKLAEEDKSGEQIKLIDQNLASFEASLAEELEAYKAKRHIAFEKSLERDLSKA
                     KPTAQELEALKSRLTADNETYTAAVNAKREAKRAKFVRSNKARIAKLEGKLNNFQAKL
                     KVQLERIQQDSYALPEGVILRAENLCMFFGGVKAVNDLTFDVKQGEIFGLIGPNGAGK
                     TTVFNCITQFNKPTSGHLYLRNRLDEVVNLNKLAVHNVILQGIVRTFQNLEVVKEVSV
                     LDNLLIAAHRQYQSNFFIHMLHLPILRLEEKVIRARADKVLAFMGLSLYRDWYAWGLP
                     YGVLKKIEIARTLMADPHLIILDEPAAGLNDSETVELANLIRRIRDEYKCTILLVEHD
                     MGLVMDVCDNILAISFGKKLAQGSAKEIQSNKDVQAAYLGAPEEVER"
     gene            complement(45568..46656)
                     /locus_tag="PLH17_00650"
     CDS             complement(45568..46656)
                     /locus_tag="PLH17_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR20322.1"
                     /translation="MNNRFKNEYLAHTSPRPESGWKAHAKRIMSNPLGQFGLLGLLLA
                     ITQLLNIAGIVPRSIMTTIAFVSIYSIAAIGFCLLLGYSGLASLGTAGFMGLGIYLCH
                     YGLQEWQISILPAFLIAGIASIVIGFVVGFVSLRIEGIYLAIMTLALSEIIVKLLKTI
                     KGSTLIIRGVNLNFFGIPLSNNAVYFTIIVVLVMLLVGTANLINSPTGRAMVAMKSSV
                     AAAQAMGISLLKYRLLAFIISTLFAGIAGFFYHAYLPGISPTTATILTLSMSLNILGA
                     VIIGGAKSLWGTLVGTFVIYGFQSIFLQNIAFFNENPAFMMMVTGVLIILVVMFYPGG
                     FAQMLFTFRLWLKKMTKKWRIYRYGIEE"
     gene            complement(46667..47581)
                     /locus_tag="PLH17_00655"
     CDS             complement(46667..47581)
                     /locus_tag="PLH17_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOR20323.1"
                     /translation="MAQLLSLLNTITGSLLTICVTMVATMGITLIFRTSNTTNFAQGA
                     IAAAGTYVVAYLAMQYSVHYILAILIGLVFGIAFGLFIDIMIFRRGKNINIVGKQIIT
                     MGLVSIIFGAVPLIFGAQIPPQIPAFIDGNFSISMLGGELSITKHSVVCIAITAAVLT
                     VLFVLLFKSKWGLGVRVTASSEYTAGIVGVNTYVITATSWAIAAALAVLAAVMLNAGN
                     ASLTPSFMTKIQVSSFLAGILGGFSTFLGPILGAIIIPLAISVVGWLGFINPFFQSWA
                     EVIVYILLLIAILIKPQGLFGKVVAKKV"
     gene            complement(47585..48604)
                     /locus_tag="PLH17_00660"
     CDS             complement(47585..48604)
                     /locus_tag="PLH17_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HOR20324.1"
                     /translation="MEKVKVGIVGVGTYLPSQVMTAKEIAAKTNGVWSEEAVINKLGI
                     RQKYIPGPDDGTQEMGAKAALNCLKNTGIDPLDIDVILCIGEEWKEYPLTTSALYIQD
                     RIGAINAWGIDVQNRCCTCVSAMKIAHDMLVADKSIDTIMIVGGYRNGDFVDYADKDM
                     SMMYNLGAGGGAIILKRDHDKNLLLGSKIIADGSLSRTAGVEIGGIAKPFNADNIEEG
                     YKSLRLLDAKKMKDRLNEVSMSNWYRCIDESLQQAKLTRKDITFLDILHIKRSGHAAM
                     IADLGLREDQTIYLEDYGHIGQIDQILSLELALKEGKVKDGDVVCMIAAGIGYVWSAN
                     IIRWG"
     gene            complement(48615..49334)
                     /gene="fabG"
                     /locus_tag="PLH17_00665"
     CDS             complement(48615..49334)
                     /gene="fabG"
                     /locus_tag="PLH17_00665"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOR20325.1"
                     /translation="MRLQNKVAIVTGGAKGLGQAMSELFVKHGAKVIAVDMMDLTYSH
                     PDVEFYKLNVANSDDVARFGEYVFAKYGKADILVNNAGITRDAMTRKMTDEQFDLVIS
                     VNLKGVFNMTRVFGPKMEEAGYGSIINISSVVGVYGNIGQANYAASKAGVIGMTKSWA
                     KEFARKGANVRCNAIAPGYIMTDMMKTVPEDLLKKFAELTMLKRLGQPHEIADAALYL
                     ASDEASYVTGQVLEVNGGMRL"
     gene            complement(49366..50571)
                     /locus_tag="PLH17_00670"
     CDS             complement(49366..50571)
                     /locus_tag="PLH17_00670"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005898377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOR20326.1"
                     /translation="MKKVYVVAAKRTATGSMLGSLKTIDATDFGAEVVKALLSATKVA
                     GTDVSEVIVGNVLSAGLGQGPGRQVSIKAGIPVEVPAYSINMLCGSGMKSVLTGYTAI
                     RDGFDEIVIAGGIESMTRAPHLVPSAVRGGIKMGDVKLTDHMIYDALTDAYSQVHMGV
                     TAENIAVKFALSRQEQDQFAYASQVKAIKAVDAGLFDDEIVPITIKDRKGDIIFNRDE
                     YPNRTTNLEKLAALRPAFVKDGSVTAGNSSGINDGAAFLMLMSEEAVQKHHLKPLAEI
                     VGIGQGGVDPQYMGLGPVAAIRDVMKRSGYKLKDMDVIELNEAFAAQSLGVLKSLAEE
                     HDIGFDELLAKTNLQGGAIALGHPVGASGARISVTLLHIMQRHDFKLGLASLCIGGGM
                     GTAIIFKKI"
     gene            complement(50587..51570)
                     /locus_tag="PLH17_00675"
     CDS             complement(50587..51570)
                     /locus_tag="PLH17_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOR20327.1"
                     /translation="MNKDILTFIEVRDGKIQKVSLELLGAARDLADVSGGHVVAGIVG
                     SNLSGLIEDLKTFDIDLIYSVDNSNLKYYLTDAYARAFEKIITTVNPNVILLGATSIG
                     RDLAPRLSARLGTGLTADCTKLEIDVNGSLFMTRPAFGGNLFATIICPEHRPQMSTVR
                     PGVMARIGANKGRLVNVIHLDVAVDKPRVEVLEEVVATQEIANIEEAKILISIGRGIG
                     GDENLKTAQYLAGLIKGTYSCSRSLVDCGKINPDRQVGQTGKTVRPELYLALGISGAV
                     QHLAGMEESDYIIAVNKDRTASIFSVADLGIVGDINQILPLLKEEIEKNAL"
     gene            complement(51583..52365)
                     /locus_tag="PLH17_00680"
     CDS             complement(51583..52365)
                     /locus_tag="PLH17_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOR20328.1"
                     /translation="MKIIVCIKQVPNTTEIKIDPVTGTLNREGAVSIMNPDDKVGLEF
                     ALCLKDAHQAHITVVSMGPPQAKKVLYEALAMGADRAMLLTDRLFAGSDSLATSLTLA
                     GLLKTLEYDLIITGRQAIDGDTAQVGPQIAEHLDIPQISYVNSLKFEDGYFIAVRQFE
                     DRYQTLKATAPLLVTILGDAAKPRSLHVGRLIEATETESVELKGASDIIVDTTKLGLK
                     GSPTKVKSTWNKVVDKQKNLLEVEPREAVKAIIDTMKKRHII"
     gene            complement(52371..53507)
                     /locus_tag="PLH17_00685"
     CDS             complement(52371..53507)
                     /locus_tag="PLH17_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOR20329.1"
                     /translation="MDTILRYELFNDFIKEFALKQVKPLATQIDEEERFPSETIEKMG
                     KAGLLGIIIPKAYGGAGGDYKMYINAVREISSYCATTGAILSAHVSLCTAPIAELGSE
                     EQKQKYLPKLANGEWLGAFGLTEPNAGTDASNQQTVAIEDGDSYILNGTKIFITNAGY
                     AHVYLIFAMTDKSKGIKGITAFIVEADMPGFSVGKKEAKMGIRGSSTCELIMQNVRVP
                     KANILGPVGEGFKVAMKTLDGGRIGIAAQALGIAEGALRETVNYVQERKQFGRPLSAF
                     QNTQFQIADMATHIDAARLLVERAITSKEQKAKTLSKDAAMAKLFSSETAMMVTNKAV
                     QLHGGYGYTREYAVERMMRDAKITEIYEGTSEVQRMVISAAVLR"
     gene            complement(53511..53915)
                     /locus_tag="PLH17_00690"
     CDS             complement(53511..53915)
                     /locus_tag="PLH17_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOR20330.1"
                     /translation="MRFEELKRGMKASISKRITAEQVDAFAQLSTDYNPLHINEEVAQ
                     KSVFHHRVAHGMLVASLFSAVLGTRLPGEGSIYLGQDLRFVAPVFLNDTITAEVEVIE
                     LREDKRIVKLATTAINQDGKSVITGTATILVR"
     gene            54195..54797
                     /locus_tag="PLH17_00695"
     CDS             54195..54797
                     /locus_tag="PLH17_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR20331.1"
                     /translation="MESSVFYPKAKRGKATFRRIITAATKVFYERGYANSTIADIAAE
                     AEVAVGTIYIYFKDKYTIYYQVLKDFQNQIREHLNRNIKEAHTRREKERAGIRAWLEF
                     VLEHRRAYEMIWESLYIDRRLFDDYYLSFSEAYRKGLEHDSKELVNVDFETVSYILMG
                     IANFVGIQAMLTADIDDQKLDAIVDSVMLILANGLFKKQS"
     gene            54926..>55720
                     /locus_tag="PLH17_00700"
     CDS             54926..>55720
                     /locus_tag="PLH17_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOR20332.1"
                     /translation="MSKEMWAYLIALAVSLLAIGVAVWMARWVKKLKSENAKIAYVSS
                     LIKSGANTFLKREYTILAVFASILFVFILIFLPKPIWQVGDEVGVNRVLPNLAMALSY
                     LIGTIFSGIAGKIGIDIATIANVKSAEAATKGIKPSFMAGFRGGAVMGMAVVASCLLG
                     VTLVFWATYGWTGNARVLIGFSFGASSLALFAKAGGGIFTKTADISADLVGKVELSIP
                     EDDPRNPAVIADNVGDNVGDVAGMGADLFDSNVASLAAALVLAIALD"
BASE COUNT        14664 a        12724 c        13391 g        14941 t
ORIGIN      
        1 atgaacgcgc cgcttggtta cacccgcgtc gatgatgaaa acgatggtga tggttaccgt
       61 ctttatcgca atgataatca catcgaatta ggctttagtt ttgacacgct ttattataag
      121 cgaagctgcg gggatggccg ttataacgat ttccatacaa caagcaatca ctcctttgat
      181 gtcatccgca atgaggaagc ttatcttaaa ggcgctattt taaatgatga agatgtcctg
      241 gatgtcgttt ccgaccaccc ccattttaat cttcgctatg caccgccatt agaggctgac
      301 cgtctaagca tcacccgcac tctctacgac accgactttt acttccaacc ccgcgaccca
      361 gaccgctatc tcgattacga gacgttcccg tttgcggaag ccgatgaaaa agtcgtcagc
      421 gacaaaacgc aacttgtctt agcgccatcc acaggcgatt atctgacgac cgaaccagcc
      481 tatatcatgt tgaaatatcc acttaaaatg ccggataccg actatcgcgg tcgcgtctat
      541 ttaatcggcg acggcatcga cgacgagggc aatcccaatc cgacgacgag tcgcgtcatc
      601 acgactgatt atcatagtaa caacgaagcc tatcagaagt atttccgcgg tttctacgcg
      661 aatgaaccgg tcaaaaagat catcgtcgtg cccgctggca atggtatcgt ttatcagtat
      721 agcgacatat atgtcgaaac ctatgatcac attctcaata aactcgccaa cctcaatcaa
      781 tatccgcttg aagatgtcgt ttatgacaca aactatttct cttttacgac agattttgcg
      841 accgagcgct tcatcgtcac ccaagtgact tatggcgacg gctggaaagt gtttgccaaa
      901 ggcgaagacg gcataagcga acagctaaag acatacaatg cccagggtgg tttcgtcggc
      961 ttcgtttcca aagccggagc gacatcatac gaaatgaaat atgtcacacc ctattttgcc
     1021 gagggcctaa tcgtcgcttt tagcggcttt gtcatcttcg gcggcgcgat tggcctgggc
     1081 gcttatttag atttaaaaaa gaagtctaaa ctagaagacc aagataaaga aaaacgccaa
     1141 taaggcgtta gttttgatga tagattttgt agacgcggtt tttggcgagt ttgagaagat
     1201 cgctgacttg tttgatggcg tccttgggcg tcatgccgat atcgatgaaa tttgagatca
     1261 tcttgcggac ttcgtcgtcg ttgatgtggg ccgccacctc ttcattatta ccttcgatga
     1321 caagaaccat ttcgcctttg agcgtctcct tgtcaagcat cgacaaatcc gtcaaattca
     1381 tgcggatgaa ttcctcgtgt ttttttgtca gttcacgcgc caggcaggct ttgcggttgc
     1441 cgaatatttt tagcatatcg gaaatcgtct ttacaatgcg gtgcggagct tcatagaaga
     1501 tgagtgtttc gcggcgttta agcagagtgc gaagctcttc ctgacgcact ttttctttcg
     1561 gcgagagaaa gccgtgaaag tagaagcgtt cagtgctgag gccagagccg acaagggcat
     1621 tgagcattgc gttcgcaccc gagatgaccg agaccttgat gccgttggcg agagcttcgc
     1681 tgacgaggcg gttgcccgga tcggaaatcg aagggtaacc ggcatcggaa aggtaagcga
     1741 ttttctgacc gttttttaaa tcgctgatga tgcggatggc gccggatatt tcgttgtgtt
     1801 cgcggcacga gactaaaggc ttctgaatct caaaatgggc gagcagtttg cccgagacac
     1861 gcgtatcttc acaggcgatg taatccattt ctttaataat atctaaagcc cgcggcgtca
     1921 tctcgttgag attgccgatc ggtgtggcga tgatatagag aatcggagta tttcctttga
     1981 agtttaactc tctcattttt tatctttgct cctcatatac tttttgacat cttcaagggg
     2041 cagaaatttg acatcttcgg atgactcgac gcggaccatc cgcgagagga cattatacga
     2101 gccgatgcga tatgataaac catcgatggt aaagcgctct ccaaccgcgg gaaaatcctt
     2161 cttcagctca gaatattgtt catcttcata aaggaggcag cagatgagct ttccgcagtg
     2221 gccgctgatt ttcggaatgt ttaaagacag catttggttt ttggcgcggc ttatcgaaat
     2281 tccgtcaaag gaatgaagaa aagttgagca gcaaagcggc aagccgcaga tgccgatacc
     2341 tccgaccatt ttcgccttat cccgcgaacc gatctggcgg agttcgatgc ggcagcggag
     2401 gcggccggcc aaaatcttga ggagttcacg gaaatcaacg cgctcttcag cgacataagt
     2461 aatcgtaatt ttggtgccgt cgagtgtgta ttcagcatcc aaaggatgca tgtcgaggtt
     2521 gaggttttga atctcctgaa ggcaatattt taacgcttcc gcagcccgct ttttgttttc
     2581 ttcaaaaaca taaaggtcgg tctcattagc tttgcgttcg atgggtttca gttcgaggcc
     2641 ggaactgtat ttagcgatat cttgcggttc gatgacgact ttggcgattt ccaaaccgcg
     2701 gacggactcg acgacgacat gatccccgag tttgatatca ttattttcgg tgccgaaata
     2761 ataagccttg gatgagctgt aataacggac accgataaaa taattgttga ttgtggcagg
     2821 catttatttc tccttcgtaa tggcgatgat gacatggtcg agcaagaggg cgacattgac
     2881 attcaaatcg agtttgccgc gcgaagtcat aatttcgatg agtgtttcat caaggtgacc
     2941 catgcgggcg gctaactcat gtaatatact atcataacta gttaaagtga tgcgattgtt
     3001 aacgcggata ttgagcaagt cctgaaaaac gatcgtcagc atatcaagat aaaagcgagc
     3061 ggcctcttta cttttgatgg cgggcacaat ttcgctttgt gaaacaaaaa tagcctcttc
     3121 tcttgagcga cgaagtccct ctaattgctt ctcaagagca ttcttggcat cgatgtattc
     3181 ttgactctga taggtgttga tgatcgtcgt cggatcattt aaaaagaagc tgagaatctc
     3241 ggcgtcttga cgcggacagc cttgcccgac ggattcttca atcaactgct gtttgtcaat
     3301 cgtctttaaa cggaggacct gcgttcgcga gatgatggtc ggcaacacct tagcctcatt
     3361 ttcgctcgtc aagaaagcaa aaacattctt gcccggttct tcgaggaatt taaggaggga
     3421 attgacggcc tccgtcgtca tgttttcgac gaggtgcaag atgtaaatca tgatgccttt
     3481 tgcctcaaga gccgtctttt cgaagttcga tgtaatttcg ctgacatcgt tctttttaat
     3541 cgtggtttcc gccccgtcgt agatcataat gtccgcaaaa ctgccgtctt cgatgcgaat
     3601 acacgttaaa cagcgctcgc aggcaagggg ctcaggcata tcgcaaacca aagatttggc
     3661 caaaaataat gcgacatctt ttaaaggcgt tccgggctct ccggaaagaa gataagcatg
     3721 ggtcaattta ttgttttcaa gggcgttaga aaaaacttta tacaaaacgc tttggtattt
     3781 tttaatgtat tcagaggcga gcatgctttt gctccaagcg atcaagaatc acattgagag
     3841 ccaccccgac aacttccgga agggtttttg aagcatcgat tacaacatag cgctcttgat
     3901 atttcttggc cagttcgaga aaggtttcgc gaacctgccg atgaaattca agagcctcaa
     3961 gatcgaggcg attgacttct cttttcttat tgatggcaat gcgttccaaa cctttttcgg
     4021 gagttatgtc aaagagcaag gtcagatcgg gaaaggtgcc ttcggtggcg aataaattaa
     4081 tatttaaaac attttcgatg ccgattttac gggctgcgcc ttgataggcc agcgatgaat
     4141 caagaaagcg gtcgctgatg acgatttttc cttctttgag ggcgggccac actttttcga
     4201 tgaggtgttg gcgccggctc gcggcataga gcaaagcctc ggcgcgtgca tctaaagcgg
     4261 tattcttttt atcgaggatg atgttgcgaa tctcttcggc aatcggcgtt ccgcccggtt
     4321 cgcgggtata taagacatca tagccgcggg cgacaaggat gcggattact tcattggcgg
     4381 cgctggtctt gccgctgccc tcggggcctt caaaggtgat aaacatagtg ttttcctcct
     4441 acaatttgcg gcgcccttca agcgccttat taagagtcag agaatcggca taatcaacat
     4501 gtcctcccat agggagtcca taagcaagtc tggtgatttc aacgcctttt ccttcgagca
     4561 acttggcgat gaaaagagcg gtggtctcac cttcaatggt cgggttggtg gccaaaatta
     4621 tttcctgaac attgtcgtct ttgattctct ccaagaggga atcgatgttt agatcctcga
     4681 tgccgatgcc ggcgacagcg cttaaaacac cgccgagaat gtgatatctt ccgcgaaagg
     4741 tattcagttt ttcaaaagcg tagacatcgc ggtatgaaga gacgacaatc aacgtctttt
     4801 catcgcgttc tttgtcgctg catatctcgc atttttcatc ctcggtataa agaccgcaga
     4861 cggggcatag atgaatcgcg gttttgacgc cttcgagggc tgacgaaaac tctttgacat
     4921 cctcctcgtc catctctaaa acggctaaag ccattctttc cgccgatttt cttccgatgc
     4981 tcggcaggcg ggcaaaagag tcaatcaatc gtgttagaga ctttaaatcc ttcattaaaa
     5041 acccatcatc ccttcggcgc ggccggtgat gcgcgtgttg atttcatctt cggccagttt
     5101 gatattcttc aaagcttcgt caatcgccag tttcagcatc tcttcgatca tctctttgtt
     5161 gtcggcttca agagcggcgg catcgatgtc gactttttta atttttctat caccataaaa
     5221 gacgacggtt acggctccgc ctttcgagac ggaaaattct tcctgggcca aatcttcatg
     5281 ggctttttta agttcgcgct gcatcttctg agcctgcgct aatagattct gcatgttcat
     5341 atagttgctc ctttaaggtt tagctgaata tcctttgctt taggcaaacg attgagctgc
     5401 ctcagattaa aataagattt ttgcaatcgt aaagcttcgc ttctgacaat ggcatagaca
     5461 aagacatcgc ggccgatgat gtttttcatc agctcgccga ttgaactttg gttggtaacg
     5521 atgttgattc gttcggccaa acagtcaaaa tcatattcga ggacgaggac gtctttggcg
     5581 acgatgtaag gtcttccgtc tttgagcagg gaggcgacga gtccgtagcg gggatgagcg
     5641 acgaggccga taaaatcatc ccattttttg ctgatgtttt gcctctcttc tttatccccc
     5701 aagaccatga tttttatcat cgtctcatca tctaaggcgt ttttatctcc ggcgagatca
     5761 atcgtgcact gacccggtga atctttgcct ttagttttga cttcttcggc cggcctgttc
     5821 ttctcatcga tggccatctc atcttctaaa agccatgatg ggacggtcga gccattcgtt
     5881 tcgataaggg gagccgcctc aatcggtgtc ggctgctgag cgtttatttc gcgttcaggt
     5941 attactgttt gaggcgcgat aacttcagct tttttcaaaa cttcttcctt cttttcagtc
     6001 ggtttaatga tttgttgtgg ctcagccttg cgcatttccg gcgtctgatt gtcctcgctg
     6061 gggatggtcg cttttttcga agatgtcgct aagcgaagga gcaccaattc gaaaagggag
     6121 cgaatgtttg aaacaacctt gaaatcattt tgagctttca gcaagatttc aatcatctca
     6181 ttaaggcgat cgatatcgat taattccttg agcgataagg cgtctttttc ggtaataacc
     6241 gagaggagga tgtgctcttc ggtccggcta tagatcaggg cgtcttttaa gatgtccaat
     6301 agattagaag tcaggcgctt gatgtcgatg cccgaggcga taaaagattc catgcgcttt
     6361 aagacatcct tgacatcgcc tttggaaata gaacggatta aatccagttt ttctttattg
     6421 gaagcaaggc caaaaagatc gaacacatct ttctctttta tcgaatttcc agcgtaggcg
     6481 aggacgtgct cgaggattga aagagcatcg cgaacaccgc catcggcgag ctcgatgatg
     6541 gcattgattg cgtgaatgtc atatgttatt ttttcgagat cgagaatttc gaccatcttt
     6601 tcgcgaatcg cgtcatcctc aacctttgtg aagtcgtatc gctgacagcg tgaaacgatg
     6661 gtcggcaaaa ctttgtgcgg ctcggtggtc gccaggataa aaacaacatg tgccggaggt
     6721 tcttcaagtg tttttaacaa agcgttgaaa gcgccgctgg tcatcatgtg aacttcgtcg
     6781 atgatatata ctttgtattt tcccttgatc ggcaaatatt taactttttc gattaaatct
     6841 cggacttcat cgacgccgtt attgctggcg gcgtcaattt cgatgacatc gggatgcgag
     6901 ccgtcattga cgctcaaaca gttgccacat tgattgcact ggtggccaaa tccctcttcg
     6961 cagttcagcg ctttggcaaa gagttttgcc atcgaagttt taccggtccc gcgcggtccg
     7021 gaaaaaagat aggcatgggc aatcttgttg ttcgccaatg cgttttcaat cgttctaacg
     7081 atgtgttttt gacccgccac ctctaaaaag gttttggggc gataagtcct atatagagct
     7141 ttatatgcca tttctttatc ctattcgtaa ttatacatat ttatgcgttt tgattaaaga
     7201 caaaaatcct cttttaatat taatattaag gcaaaaaaca aacactttat agaaaagacc
     7261 ggatgtggta taatctcacc gctgttgagg agatcatatg gaagatagca tgctgaaacg
     7321 cctggatgca acgaagaagc gcctggccga aattgatgaa gaacttgctg acgaaagcgt
     7381 catgcgagac atttctcatt tcaagaatct ttccaaagaa cggtcgaata tcgccgatat
     7441 ttgcgagaaa tatgatgagt acagcaaata tgcagccgaa cgcgaagaag cgttagcgat
     7501 gatgagcgac agcgatagcg aattagctga actcggcaag caggaatttc actccctcaa
     7561 tcagaagtta gaagatatcg aacaagaatt gcacttgatg ctcgtcccca aagaccccaa
     7621 tgacgacaag aacattatcg ttgaaattcg cggcgcggtc ggcggtgatg aagccaatat
     7681 ttttgccggt gatttatttc gcatgtatac gcgatatgcc gagcgcaaca actggaaaat
     7741 tcaaatgatt gatgaaaatt catcaggcac cggcggcttt tcccttgtct ctttcatgat
     7801 taaaggtgac ggagtttatt caaaactcaa atttgaaagt ggcgcgcatc gcgtgcagag
     7861 aattccccgc accgaggcct ctggccgcat tcacacctcg acgtcgaccg tcctcgtcat
     7921 tcccgaagcc gatgaaatcg aagtcgtcat caatcctggc gatttacaag ttgacactta
     7981 tcgatcgcaa ggggccggcg gccaaaacgt caataaaacc gaaagcgctg tccgcataac
     8041 ccaccttccc agcgggattg ttgtgtcctg tcaaaccgaa aaatcgcaaa tccaaaatcg
     8101 cgaaatcgcc atgagaatgc tccgcgctaa aattctcagc gacttacagg cggcacagga
     8161 agaaaaaatc ggcaatgaaa gaaagctaaa ggtcggcact ggcgaacgca gcgaaaaaat
     8221 cagaacttat aactatccgc agaatcgcgt gaccgatcat cgcatttctc tctccgtcca
     8281 aaaattagac cggatcatgg aaggcgatct cgatgagatt ctcgatgcct tagtccatta
     8341 cgaccaacag caaaaaatgt tgggcgaata atatgccgac tctttttgaa ctatatcaaa
     8401 acatcatccg cgagtcgcca tttgcacaag cgaatgaagt tgctttgcgg gcgctccttt
     8461 gtgagattca gggccttata gagatgtctg atttttatct taaaaaggat gaagaaatta
     8521 aggatttgcc tacttttctt tcatatttag ctcgtttttt gaatggcgag ccgtatcagt
     8581 atattataca aaaagctcgt tttatcaatc gtgatttctt cgtcgataat cgcgttctta
     8641 ttccgagaat ggaaaccgaa gaagtcgttt ataaagccat tgaacttatt cgtgatagca
     8701 acaattcgtc aagtccttca attgctgatc tgggcagcgg atcgggaatc atcgcaataa
     8761 cattaaaaaa gacatttcca cactctcaag tttttgccag tgatgtttca aaagacgcta
     8821 tcgaagtcgc caaaatcaat gctgaaaagt ttggcgccga catctctttt tttcgcggtg
     8881 atgtcctgga accatttatc ttttccggaa tcaaactcga ttttcttgtc agcaatcctc
     8941 cttatataac aaatattcag gaaattgatt ccggcgttat caaatatgaa ccttattccg
     9001 cgctagttga taaagatgaa ctcggcttct ataaaaaagt cttttcaatg tataaagcgg
     9061 tcgctaaata cccattcacg atgatttttg aaatcggcca cgacatgaaa gataaaatca
     9121 cgaccttact tggaaccatg attccgctcg caaagtggga gattatcaag gacattaatg
     9181 gtaaagagcg gattctttct atttatatag aaaagtagta atattaaaaa catgaaaact
     9241 atttcaatca gcgatatcaa tcttgcttct cagttgctaa aagaagaata cattgtcgcc
     9301 tttcccaccg agacggtctt cgggttgggc attatctatg attcagaacg cgcttttgaa
     9361 catctcgccg aagtgaaaag acgcaggcca gacaagccct ttactcttat gtgcgccgat
     9421 atttctgata tcgacaatta cgcggttatc aatgaacggg caaagcgcct tatcgcccat
     9481 tttttaccag gagaactaac gattgtttta aacattaaac ccggccttcc tcaatgggtg
     9541 gattttggca caggacacat cggcatccgc gttcccgatg acgagattgt gcgaaagatg
     9601 attcgcctcg tcggcaagcc tttgctcgtg ccgagcgcaa accgagccga tcagcggccg
     9661 ctgacgacga gtgatgaggt aatatccgaa tttggagaag aaatcggcgg tgtcgtcctc
     9721 gggcgatcaa aaggtgcaac gccttcgaca gttatcgccg cttatgataa aattactatg
     9781 ttaagggaag gaaaaatttc cttggccgac attacaaaag tattgggtgg tgattaatat
     9841 gcgcattgta gttgcttcag accacggcgg attcgaatat aaaaatgaga ttcgccgaca
     9901 tcttgaaaaa cggggctttc aagtcctcga tgtcgggaca ttttctaatg aatccgtcaa
     9961 ctatcccgat tatgccgcag ctgcggcaaa aaaagtcgcc aatggcgatg ctgatcgcgg
    10021 tattttagta tgcacgagcg gtgagggagt gatgattgcc gccaataagg ttaaaggcgt
    10081 tcgcgccggc attggttata acgataccgt ctcgcgcctc ttaagagagc acaatgatgc
    10141 gaacgtcata tctttcggcc agaaatttat ggagctcgat gacgtgattc gccgcgtcga
    10201 tatttttcta gagacgccat ttaacggcgg ccggcatgcc aaccgcgtcg aaatcatcaa
    10261 gaacatcgaa aaagacaaat aaacaacaaa cattgataag gctccaatat taattggggc
    10321 tttttcattt ttgatcactt acatatcagg tggtatgttt aagtgttcaa gatgcggaaa
    10381 taaagatcct cgctacatcg gctataaaaa cgagcagccg tattgccgtc gctgtatcat
    10441 tttttctggt tcaggcgcca aggaaaacga tggtaaaaca taccagtcaa aactcgagct
    10501 tcactacgaa ttgtccagcg aacaaacgga agcttcaaac cgcatattaa acgctataca
    10561 taacggtcga gatgtcttaa tcaacgccgt gtgcggagcg ggaaaaaccg aactcgtttt
    10621 tgccgtgatt aaagaggcgc tcatcaaagg acggcaagtc ggttttgcca ttccgcgccg
    10681 cgatgttgta atcgagattc acaagcgtct taaaaccgtt tttcctcgca acaaaataat
    10741 agctgtttat ggaggaaata acgaagaatt gcagggagaa gtcgttgttt taacctctca
    10801 tcaactatat cggtacgaat catattttga tcttttaatc atggatgaaa ccgatgcctt
    10861 cccttttcac ggaaaccctg ttcttgaggc gttgtttaag cgcagttgtc gcggaaatta
    10921 tgttatgatg tcagccacat taaccgaaac ccaaatcaag aaattcgcgt caagcggtgg
    10981 cgagattatt tctcttgatc gacgctatca cggctacccg cttccggttc ctgacgtgat
    11041 tctcctgctc ggcattctca agcacttttt tctgattcga aaactaagat cctttctcgc
    11101 cgagaacaaa ccggttttcg tcttcgctcc gacaattgac aagtgcgaag atttatctgt
    11161 ttttgtcaca aagtgggtgc gaggaggtac ctatgtccat tcaaaacgtt tggaccggca
    11221 agaagtgatc aaagattttc gcaaaggaaa atataaatac ttggtgacaa ctgctgtttt
    11281 agaacgcggc gtcaccgtca aaaaccttca ggtcgtcgtc tttgaatgcg atcacgaaat
    11341 atattcagcg agttctttga tacagattgc gggaagggtc gggaggaaga aagatgctcc
    11401 acaaggcgaa gttatttttt tgtcagatcg gaaaactcgt gcgatggaag aagccattta
    11461 ccacatacgc gccaaaaaca agagtatgcc gaatgtgctt tgaaaaaatc aaagaagata
    11521 catttcatag ccttgtccac gccgatcttt gcatatgcca aaaatgtcag gatcagttcg
    11581 tggccatctt tgaaaacaag atcattaacg acgtcaaagt ctgcgccctc tacgactata
    11641 acgaattcat tcagtcgaat ctctttaaac tcaaaggatg cggcgatatt gagctcgctc
    11701 cctgctttct gaattatcat ctaccctggc ttatggctcg gtatcgcggg cacatcatcg
    11761 ttcccgctcc gagttatggc gatcgcgatg ccaagcgagg ttttaatcaa gtcgaagaga
    11821 tttttcggag ttcaaaactg ccgcttatca aggccataat taagactgaa gatcgcaagc
    11881 aatcggactt atcgcaagac gagcgaaata aaataaagga gataattgct tggaaagagc
    11941 aagctgacat ctcgggcaaa aaagttctcc tcgtcgatga tgtcatgacg acggggtcaa
    12001 caattcgggc ctgcatcgat ttgattaaac atcaccatcc aaagtcacta gatgtcctcg
    12061 tgatttctag ggtggcaaat agcgagcaaa gataagtttg gcataccatt atgtttaatg
    12121 gtaaaataac atggctaaat aaatagagtc ggagtgtaga aaatggcaaa ttttattgaa
    12181 aaaatgttcc gttttgatca aagaatatta aagaaatatt ttcgtgagag cgaaaaagtt
    12241 cttgcttatg agcaagaaat ggccgcgaaa agcgatgacg atctgcgagc taaaaccgat
    12301 gagtttaaac tgagattaaa aaatggtgaa acaacccagt ccatcaaata tgaagccttt
    12361 gcggtcgctc gcgaagcggc gcgcagaacg cttggtcagt ttccctttca ggtccaagtt
    12421 atcggtgctt tagttctcga tcaaggcgat gtggcggaga tgaaaaccgg tgaaggcaag
    12481 actctcaccg cgaccatggc cgtctatctt aacgctctta aaggtagcgg tgtccatgtc
    12541 gtcaccgtca acgaatacct cgcccagcgc gatgcggact ggatggggca gatttatcgt
    12601 ttcctaggtc ttaccgtcgg ggtcaatatc cgcgagaaga cgaacagtga gaaaaaagaa
    12661 gcccataact gcgatatcac ttatacaact aattcggagt taggctttga ttacttgcgc
    12721 gacaatatgg cccaaaacgc caattcacgc gttttgcgcg gactcaacta tgccgttatc
    12781 gacgaagccg actcgatttt aatcgatgaa agccgcaccc cgttaatcat ttcaggcggg
    12841 gcgaaagcca gcgcctcgca atatacggtg gcggaccgct ttgtcaaaac attgaaaaaa
    12901 gaacgcgact ttgaagtcga catcaagacg aagacgtgca gcctgaccga agccggtaac
    12961 acccgcgccg agcggatgtt tggtattcgt aacctctatg atcccgaaca ccaagacctc
    13021 gttcaccgca tcgtccaggc tctcaaagcg aactatatca tgacccgcga tgttgaatat
    13081 ttagtcgacg gcgagaatca aattcagtta atcgatcagt ttactggtcg tattttaaaa
    13141 ggccgcgagt attccgacgg gttgcaacaa gccattcaag ccaaagaaaa cgtcgagata
    13201 aagcaagaga cgataacgct tgcgaccatc acttatcaaa atttcttccg cttgttcaaa
    13261 aagctttcgg gcatgaccgg aacggccaag accgaagagg aagaatttcg caaaatttac
    13321 aatatgagag tggtggccat tccgacgaat cgcccggttg ttcgcgatga tgcaaccgat
    13381 tatatctacg ctcataaacc cgctaaactc gaagctctcg tccaagaagc taaagaacgc
    13441 catgagaaag ggcagcccat cctcatcggc accgtttccg tcgaatcttc tgaagaggtg
    13501 agtcgcctct tgactgccgc cggtttaaaa cacgaagtcc tcaacgctaa aaaccacgct
    13561 cgcgaagcgg aaattatcgc tttggccggc aacaaaggag ccattacgct ggcgacgaac
    13621 atggccggtc gtggcaccga cattaaaatc aacgatgaag ttcgagccct tggcggacta
    13681 tgtgtcctcg gcaccgagcg ccatgaggcg cgccgtatcg acaaccaact tcgcggtcgc
    13741 agcggacgtc aaggcgatcc cggtttctcc cgcttttatg tctcgtttga tgatgaactg
    13801 atgcgtcgat ttggagcgga aaatgttcaa aagcgcatcg agagtctcgg caccgaggct
    13861 cttgaaagcc gcctcttctc caactttatc actagtgctc aaaagcaaat tgaaggcaag
    13921 aacttcgata ttcggaaaaa tctgctcgac tatgatgatg tcatcgccaa gcaaagaacc
    13981 gctatttatg aaaagcgcga ttcgattctc ttcgctgaca atatcaatga tttaatttat
    14041 gatttctttc agacggccgg attgttctta gccaagaaag caatctcttc ggaatcaaga
    14101 gacgggacga tcagcggcga aagcctgcgt aaaatcgtcg agccgcgctt tatggctgaa
    14161 ggcactttta gagccaatgc ctatgacgaa gctcccgccg acgaagcggg gcaagattta
    14221 ggcgataccc tctatgaccg ctatgttaat cggcgcaaag agtggggaga agaaattgcc
    14281 aaccaggtcg aacgccaaat cgccctgcag acaatcgatc agaactggac gcgccacatc
    14341 gatacgatgt cgcgtcttcg cgagggcatt tacttgcgct catatgctaa cacgaacccc
    14401 ctgcaagact atgttaatga aggtcacgcc ttgtttcgcg acatgctcga taaagtgtca
    14461 gccgatgttt gtttaaagct tttaaatgcc atcatacaaa taaaaaagcc ggaagaaact
    14521 agtgtacccg atgacaaagt catcgataca ccagcagaac cggtccggga aaaggaataa
    14581 tgaaagagtt gcaagaactg attttcgttt tcagcgaatt gaccaaagca gttaatcggc
    14641 tcggtgattc tctttgacgt cgccagttta gaagtcaaac agaaagagct ggaagccatt
    14701 caaaacgaat ccggtttttg gaatgaccaa aaacgtgccg ttagtgttgt cagcgaatac
    14761 aataaaatta aagacatcca tgatgcttat ataaaaataa cgaaaagtca tagtgattta
    14821 gatgaattga ttaaagttac tagcagcacc gatgaggata tgattctact catcgaagaa
    14881 atgctcaatg agctttcgca agatgtaaaa aacctccgtt cagaattgta tttcacggga
    14941 aaattcgatc gtctcaacgc catcgtgaca atacatcccg gcgccggcgg caccgagtcg
    15001 cacgactggg ccgacatgct ttatcgcatg tatgttagat gggctgaaaa acgccgctat
    15061 aaaatgcaag tcatcgacta ccaagccggt gaagaagccg gcatcaaaac ggtgaccttg
    15121 gcaattcggg gaaaaaatat ctatggacaa ctgaaaggtg aaaaaggggt tcatcgtttg
    15181 gtgcgcattt ctccttttga ttcaaatgcc cgccggcaca cttcttttgc ctcagtcaat
    15241 gtcgttccgg aattcgacaa tgaaatcaac atcgaaatta atgaaaacga tttaaaaatt
    15301 gatacttatc gcagcggcgg cgccggtggc caaaacgtca acaagaccga aagcgccgtc
    15361 cgcatcacgc acctgccatc aggaatcgtc gtttcctgcc aagtcgagcg ctcgcaaata
    15421 tcaaatcgcg agatggctat gaacatgctc aaaggccagc tttatgaatt ggaacagagc
    15481 cggcaaacta gcgaattaaa gagtattgtc ggcgagcaaa aagccatcga atggggcagc
    15541 cagattcgct cctatgtctt ctgtccctat actttggtaa aagataatcg cacgaattat
    15601 tctgagggcg atgtccatgc tgtcatggat ggaaacatcg acggttttat caacgctatg
    15661 ctcgatatgg aagcgcggaa taaaaaggat attttgtcat aaaaatgcgc tttttgcatc
    15721 gaaattactg gtaattgcat acttatggac acacataaat ttaatttaat ctcttcgtat
    15781 aaaccgactg gagatcagcc gttggccatc aagcagctcg tggctggact tgccgaaggg
    15841 cgccccattc aggttctttt gggagcgacc ggaaccggaa aaacatttac ggcttccaat
    15901 gttattcaag ccgttcaaag accaaccttg gtactcgtcc acaataagac tttagccggt
    15961 cagctttatg gcgagtttaa ggaactcttt cctgaaaacc gcgtcgaata ttttgtttcg
    16021 aacttcgact tctaccaacc ggaagcgtat cttccgaaat ctgacacata catcgataaa
    16081 aatgccgtga tgaatgaaga gattgagatg ctcagaacca gcgccattaa ctcggtgctg
    16141 gaaagaagag acacgatagt cgtggcttcc gtcgcctcga tctatggtct caccgatccc
    16201 gatgaatacc gcaatctcgt ctttgaaatt cgcgtcggtg agacaatcga tcgcaaagcc
    16261 ctttttcaca aattgatcga ttctcagtat cagcgtaata atatggatct cgtccctggt
    16321 tcttttcggg tgcagggaga tattattcaa attgttcctc cccacactgc tgaatactat
    16381 ataaaaattg atacatttgg tgacgagata gagcgaattg ccatggtgga tattttgacc
    16441 ggagaagtaa aaagaactta tcttacctat ccgatattcc ccgcttacgg ccatgcctcg
    16501 acaaggcaga gaatcaaaga agcgaccgtc acgattctcg ccgaactcga agagaggctc
    16561 gcatattttc gcaaggaagg aaaacttctt gaagcggaaa ggcttgagat gcggacgagg
    16621 caagacgtgg agagcatgct cgaattcggc atgtgtcccg ggattgaaaa ttactcgcgc
    16681 cacatcgata agcgccagcc tggtgaaagg ccttttaccc tcatcgatta ttttcccaag
    16741 gacttcatgc ttataattga tgagtcacac gttaccctcc cgcaggtcaa gggaatgtat
    16801 aatggcgatc gttcacggaa attaacgctt gtcgagtatg gttttcgcct tccgagcgcg
    16861 ctcgataacc gccctttgaa tttcagcgaa ttcgaagcga taatgccgca gaccatctgc
    16921 acctccgcga cacctggaga ttatgagtta gagcgcgcta atcgcgaagt cgtcgaacaa
    16981 attattcgtc cgacaggact cgtcgatccc cgcatcgaag tgcgccgcac actcggtcag
    17041 atcgatgact taatctatga aatcaagcag cgcattcttg ctaaagaaag aacgatggtt
    17101 gttactctca cgattcgcat ggcggaggat ctgaccgctt atcttaaaga aagaggcatt
    17161 aaagtcgctt atctccataa tgagaccaag actctcgagc ggaccgaaat catctatcaa
    17221 ctgcgcaagg gaaaatatga tgttttagtc ggaatcaacc tcctccgtga aggccttgat
    17281 attcctgagg tctctttaat tgccatcctc gacgccgaca aggaaggatt tctccgctcg
    17341 tcgcgatctt tgattcaggt gattggccgc gccgcccgca acaaacacgg attggtcatc
    17401 atgtatgctg atcgcatgac cgactcgatg cgtttcgcca tcgctgaaac cgaccgccgg
    17461 cgcagcattc aggcttcata taatgaagaa aacgggattg ttcccgaaac aattattaaa
    17521 gatattcgtc cgccgctaaa aaacatggag gatgaagaag aagtcctcaa ggtgttcacc
    17581 gccaaggctt ctcgcagcga aatcgaagcc cgcatcaagg aactcgaaaa acaaatgcgc
    17641 gaagcggcaa aaggctatga ctttgaaaaa gccgccgaaa ttcgcgatat tatcttcgaa
    17701 ataaaagcca ccctcaataa ataaagcatt tgctttaaga aaagctttgt gcgaatatat
    17761 taaaagcata ggtgcaaaag gagatttgaa caatgcaatg gtatgatgtt atattcctaa
    17821 tcgttgcctt aattatcatt gtcttgtcac tattacaagg cggtaaatca gaaggcgctt
    17881 ccggagctat caccggcggc ggaaccaata tttttgtgaa aacaaaagaa agaggttccg
    17941 agcttgttat cacatggcta actttcggtt ttgctatcgt cttctttttc tttgccatct
    18001 taatcagcgt gctgcaaaac gtcgcttagt ttgatcttga atttatcgag ccccctttgg
    18061 ggctttttta tttttagtgg cgatgtcgta taatatgagg cggtgattgt taaatgaaaa
    18121 attttttgaa gatgcatctc tggcgactgt tgattatctt tttccttttt cttgttcaga
    18181 tttccatcat cgtcctcggc atcttatata ttcgcgatta ttatccgtat gtctatcttg
    18241 gctttcttgt cgtcagcatc atcatcgttc tttcgatcat cgccaaccgc agcaatccga
    18301 gctataaaat tgcctggctc gcgctgatat taatctttcc ggttttcggc ggattcatgt
    18361 atctgatgtt cggcaacaaa aaaatgaatc gccgcagcaa gaaaatggtc agcaaaatga
    18421 acgccatgat cgaagcggcc accaagaaga ctgaaagcgt cctcgacgat ttggcacgcg
    18481 atcatcccga tgcccataaa cagagcacat atatcaaaaa tacgaccggc tttggccctt
    18541 atcaaaattg ttatcttgaa tactatcgct ccggcgaatc atttttggcg acgctaaaaa
    18601 gggagataaa aaaggccaaa aaattcattt ttattgaata tttcattctc gcccaaggtc
    18661 aggtgtggga tagcctttta aaactgctcg tgcaaaaagc caaagaaggc gtcgatgtcc
    18721 gtgtcatcta tgatgatatg ggttcgattt caactgtgcc gcacaattat gatcgcaaac
    18781 tgcaaaaaat gggtatcaaa gccttcgcct ttaatcgctt tctgcctttt ttaaatgccc
    18841 gtatcaacaa ccgcgaccat cgaaagatgc tcgtcatcga tggatcgacg gcctttaccg
    18901 gaggcattaa tctcgccgat gagtatttta atctcaaatc gcggttcggt tattggaaag
    18961 ataatgtctt gctaattcgg ggcgacgcgg ttcgaagcat gactttaatg tttctgacgt
    19021 tttggagtgt tctgagcgga aagattgaag aattcgaacc ttatttagca aagagtaagg
    19081 agcttaaaat tgtcgacacc ggctatgttc agccgtatgc cgatacgcca cttgacgacg
    19141 aagcggtcgg agaaaatgtc tatctaaaca tgatgggaag agccaaaaag cgcatctata
    19201 tcacgactcc atatttgata cttgataacg agatggtcat cgcattttct aatgccgcca
    19261 agcagggaat cgatgtgcgc attgtcaccc ccggaattcc ggataagaaa atggtttttc
    19321 aagtcacccg ttcctatttc cggaggctga ttgaagctgg cgtgaaaatc tatgaataca
    19381 tccccgggtt cattcatcaa aaagtgtttt tggccgatga tgatttttta acgatcggaa
    19441 cgattaattt ggactttcga agcctctatc ttcactttga aaacggcgtg tggctctata
    19501 aaagcgccat cattcaggac gtcagcgccg attttgatga gattttggct gattcccgcc
    19561 tggtcacgtt ggccgacacc aaaagcgggc ccttccatcg cctgtggatc agcttcctgc
    19621 gcagttttgc cacgctcttg taatttcctt ctttgttata cataagagcc gtgataaatg
    19681 ttaaaatatg gcatattaag tgagggttat cgcatggaaa ttaaagaata tgtcgcaatg
    19741 cgcaaacagc aaatccgcga cgaagtcgca tcgatgaaag tcagacctca tttagccatc
    19801 gtccaagtta acgaagacga aggatctaac tcttatatcc gcggcaagct aaaagatgcc
    19861 gaggaactcg gcgttttagc cacccacatc aaactggatg tcaacactag cgaagctgaa
    19921 gttttaaaag tggtggaatc gctaaatcaa gatagcggcg tccacggttt tatcgtccag
    19981 atgccgcttc ctaagcatat cgacgagaat aaagtaaaag tggccatcga tccgcgcaag
    20041 gatgtcgatg gctttcatcc gctgtcatct ttaaaagcct gcaccccgcg cggaattctc
    20101 gattatctga aatttaataa agttgagctc ttgggtaaaa acgccgtcgt catcggacgg
    20161 agcaatatcg tcggcaaacc gatggccagc ctcttaacaa aagagagcgc caatgtcact
    20221 gttttgcatt caaagacctc accccaggat atgaaatttt acttggaaca cgccgatata
    20281 atcgtagtgg ccatcggtaa gaaatggctt atcgacggtt acctttttaa accggacgcc
    20341 atcattgtcg atgtcggcat caatcgtgtg gacggagtcc tttatggcga tgtcaaaccc
    20401 ggccagaagg tacgcctgca aacacctgtt cccggcggag tcggcccttt gaccagactg
    20461 gccctctatg aaaatttaat ggagatcatg aaacatggaa tttaaaatca cgaatacgca
    20521 agtctatgga cttgaacgcg ccattaaggc ctcgggaaat ccgatgcgcg tcgaaattga
    20581 tacgagcgaa atcaatgaca aggattttgt ccgcgccacg aaactcggca tagccaagtc
    20641 gggcgagggc cacgacaatt ttctaaaggg aattcttgtc caaatggatg tgacggcccc
    20701 cctcttctgg tggaaacagg ctcagcgtta tcattggttt gactttatct catcgcaatc
    20761 gacgatgcac tgccttttaa agtttgatgt taccaaacaa tgcgttagtg acacaaatga
    20821 agaagtcgtt cgcatcgtca gcgaaatgat taaagaatac ctttcgctta gcgaagatga
    20881 taatcgcaag aaaacgctgt ggcgggaaat cgtcgcctct ctcccctctg gtttttgcct
    20941 cggggctact atgaccacaa attatcagca actcaagacg atgtatatgc aacgccgcta
    21001 tcacaagctc aacgagtggc gcgtgtttgt cgagtggtgt gatacccttc cccactttaa
    21061 agaactgacc ggcgtcagca ggtagaaaaa agaaccgatt cgcgtcggtt cttttaactt
    21121 gttgcttgct atttaacttt gtcgactggg aaggcctcgc cctcttcgac gatgaccggc
    21181 tcaacaactt cctttttgac aactttgccc ttggttttcg cctcggcctt tttttgtgct
    21241 tttttatcgc gttccttaac cttcatcaac gttttgatgg tgaaagcaat ctcgacgatg
    21301 gcgaggatgg cgaggatggc cccgatgacg atgaggacga cattctgggc attttgaatc
    21361 gacacgagga tgatgacgcc gatggtgatg aacaacaggg cggcgatgaa ttcaagaacc
    21421 ggaaagaaga gcgtctcctg ctttttcttg atgcggataa ttccgtcggc gattgcgaca
    21481 ggcgatataa cgagtaatag agtcgcgata aaaatcatga gaatttcggt gatggccgcc
    21541 gagcgaagaa cgatgacaat accgaatgtt acttcgaaaa ttcccgtaaa caacgaatta
    21601 tcgcctggat ctttgttaaa aaagggcatt agaactctca ggacgccgtc gaggatgatg
    21661 aaggctccga tggctaggaa aatccattta tagagatcgg gatcatcgga aaagataatt
    21721 gtcaaaacgc ctgccgccaa aagaataacg gcttcgcata tttgccaaat ggcccatact
    21781 ttgtacattt ttttcatgat ttcggactcc ttgcttatta atttattata ataaattgac
    21841 actctctatg atacaataac gtacgtataa aatacgagag gtataaatat ggaagttcaa
    21901 gttttacgga agtacgccga gttaattgtc aaagtcgggg cgaatgttca gaaagggcaa
    21961 gatgtcatcg tctatgctga gctcgatcag cctcgcttcg ttgagtttgt cgtctccgag
    22021 gcttataagg ccggcgctaa atccgtcacc gtccgctggt cgcacaatcc cgtcgacaaa
    22081 attcgttata aaaaggaatc gctaaaatct ttatctgaag ttccaaactg gattatcgag
    22141 cgggaaaaac attatgtcaa aacccttccg gcgaccatct atctcgtcag cgatgaccct
    22201 gatgcttaca aaggcgttaa ccaagccaag ctcgctaaag tcggtcaagc cgtttatccg
    22261 attttgaagc ctttccgcga tgaacgcgag aataaatatc aatggacgat cgccggcttg
    22321 ccaggaccgg cttgggccaa aaaggttttc cctaatcttc ctaaaaagaa agccatggaa
    22381 aaattatggg gagcaattct tgcatcatcc cgcgctgacc acggcgatcc agtcgccaac
    22441 tggcgcgccc acaacgccga tctcataaat agatgcgctt ttttaaattc tcttcacatt
    22501 aaatcgcttc attacaaaag caagaacggc accgacatca aaattggcat gctccccaat
    22561 gttaattggc tcggaggcgg tgataagacg ctcggatctg acatcttctt taaccccaac
    22621 attccgactg aggaatgctt cacgactccg gaaagaggca aggccgaagg gattgtttat
    22681 tcaaccgcgc ccctttcata tcagggtgag ctgatcgaaa acttccacat tgtcttcaaa
    22741 gatggtaaag cggtggaagt tcatgccgag aaaaatgaag ctttgcttaa acaaatggtg
    22801 tcgatggatg aattcgccgg ttaccttggc gaagttgctt tagtgccctt tgactcgccg
    22861 atccgcaact cgggtattct tttctggaat actctttatg acgagaacgc ttcgtgtcac
    22921 tttgccttag gcatgggttt taccaatacc gttcaggatt atggtaaata cagcaaagaa
    22981 gagctgacta aaatgggcat taacgattcg atgattcacg tcgacttcat gattggtagc
    23041 aatgatcttt cgatcgtcgc aacgactcaa gacgacaaaa aagtccaaat ctttaaaaac
    23101 ggaaattggg ctttttaacc ataattatcg cgtaaaaaga aaactcacga ttcaatcgat
    23161 tcgtgagttt ttttgttttt gctaatggcc gcccccgcca ttgcctccgc cgttaccatt
    23221 tccgccgtgg ttattgccgc gaccctcagt gtgggttgtg ccttggcctt gtccgtttgg
    23281 tctaataacc atttcgtatt cgtattcacc agtttcgcta ttggttacga tttgaatctc
    23341 tatcgttccg ttgtatattt ctttgtctaa cttaatatga tagcgaacgg aaatcatatc
    23401 gggagtatca tcttcaatag agatgtcgta tgaataagag tttttctcgt cggtgtcgat
    23461 gaaatccaat ttgagttttg tctttccttc ggaggtttca atttttagtt ttgtgttgtt
    23521 gagcaaatta tcattttcaa agattttaaa gttgtatttg tagccatctt cttcactttt
    23581 ataagtggat tcaaggtaat ttatctcatc gtagtgaata gtcattacca ctttggtttc
    23641 gtcttcctcg acgatgcggc ggccttcgat gacgtactcg tttgtgtcat cgccatagat
    23701 gatgatgccg gaaaaataat aagtcgactc cgcaccatct tctataagag tttcgacatt
    23761 gtagtaaatt gcataattta aatggcttcc aaggagatca taggcaatca cggtctcttt
    23821 ataatcatat ccttctcgtt ccgagatatc gctgatcgtt tctagttcgc catctgaaga
    23881 aataagttgt tcaatcattt caaggtaagg cgggaattga ccaatcacaa catcgacatc
    23941 tgtgtcgttc accagagctc tgaaatcacc gcttgtgccg gagtcttcga caaagtcgaa
    24001 taaaacattg gcagtcacgg ctccaacaga aaacacctgt tggtcgtttt gaatcggctg
    24061 cggcaccgta accggatgac tcccggtatc agtcccagtg ctcgtatcgg tcggatcagt
    24121 ggtgaagaag ccgccgattc tcaatccgat taaagtgacg gaaagaataa cggcgaccat
    24181 cgccgtggca aacgccgaaa caaagcgagg acgctgaaaa agagctttaa gagagaggcg
    24241 tggtttttct tcggtcgcag agacggcgat tttcttttgc ggaacaaaaa cttcggcctc
    24301 gctttttaaa cgctgctcga taattctctc cttctcgcta atggtaatcg gattgacgcc
    24361 gatcgcctta tagacattgt tcaaaatatc aggagtgatg atactgcctt cttggcggag
    24421 cttttttaag atttctttct tacttctttt cattttcttt ctcgtaagca atcctgattt
    24481 ttttaagcgc cgcgttatac ttacttgtca cggtgcccaa agggagattc agcatttctg
    24541 cgatttcccg gtgtttgtag cccccaatgg catataagag aacgatattc atttcgcctt
    24601 cattaagaac gcgattagct aagtcgatgg tcggagtgtc taagttcgga tttagatcat
    24661 aaacaccttg tgtctcgacg ttttcgtcat tgaagtcgta gcttatttcg cgcgagcgtt
    24721 tttttacttc gcctaaagcc agatttttgg cgatggtcag aagccagttg cgcggattgg
    24781 tccccggcgt atacgatgag atattttcgt aggcgcgaat gtaggtgttt tgcatcacat
    24841 cttcagcgac gtcataagcc ttgacgatcg gcaagacaaa cgtgaagatt ccttttgatg
    24901 tcaatcgata gatagcgtca agacatttga catcaccatt ttgcaaacag atcattgccc
    24961 gttctaattc gcttgccatc tcttttttcc ttgttttgaa ttgtaaactt ttatgaagtt
    25021 tatttcaatc gaggaagaag ggctcagaac gatttaatgt tttgggggca accgagccct
    25081 tctagaggtg tagtcacggg ggatgactac gtaatataaa tgatttatgt ttgagtttta
    25141 ttgaattatt ttaaaatttc taaatttatc agattgttaa aagaaatagt ccttgattcc
    25201 gttttaattg tacaggactc acacgagact ttttgaatcg cctcctcaag cgtcagaaca
    25261 tagccatctt catagaaggt aatttgaaaa cacttgcctt tgtaatcatg gagatatccg
    25321 tcgatttttt cggcctgatc acggctgatc tgcggcttgg ggcacttgcc tttttgatat
    25381 ttcatctgtt gcaacgaggg cttctgttct tctaaagaag aaaaaggccg ccatttcttc
    25441 attccgcggc cactcattta cggtgtcctc cgatttgggc atggcgtttg atcgcatttg
    25501 actcctcgag caaggcgctg ccgcgcagga cggcatctct gccaaaacgc aggacgattt
    25561 catcaagggc cttgcgcagc tcccttttat tcgtttgctt attcttctcg tcgaatagat
    25621 caagctgttc gaagtcatca aaactcaatt ttccaaaaga gatatcgacc cggcgaatcg
    25681 gcttatcttc gcaaaattta cgaaacagag ttatcagacc gtcaaaaatc aaatcatttt
    25741 cgctcgttga tttaactaac gtcaactgac gggagaatcc accaccgaac gaataagagt
    25801 agccgattcc caacgcgacc aagcccgtct tttttccttc gaggcgaagg cgaagagcta
    25861 agtcatcgct catctcgcgg atgatggtcg gcatttcatt tttcgcataa tcgcggaaaa
    25921 gcgattggcc atttgaaagc gatgtttctt taggaatata tttttcgcgg atatcggaat
    25981 catcgattcc gttggcgtgt tcgcgcaagt ctcttccgat ggctccaaaa tatttgatta
    26041 aaagtgcctc gggagcattg gcgagttccc cgattgttac gatgccgatt tcgtttaggc
    26101 gtttttccag gcggctggaa ataccccaca tttccgatag cggagagacg ggccacaact
    26161 tagttttaat atcgtcttca gtccattcgc ctatgccgtc tttagctttt ttgccctcga
    26221 attcaagggc tgatttggcc aggaataaat tctcgctgat gccagcggtg gcaaacagtc
    26281 cggtatcctt cttgattttg tcgagaatct tgcgaacgag ttgccgcgga gttgatttat
    26341 aaagtttgag ataagggccg atgtttaaaa acgactcatc aattgaatag acgtgcaaat
    26401 cgtcttcccc gacaaaatcc aggaagattg agacgacttt tgccgacatt tcgatatagc
    26461 gggccattct cgggacggca aaaatgatgt catcacgttt tggcaaatcg cgtttgcgga
    26521 ggcgcgaagg aacgccgaga gcttttaaaa aaggactgac cgacaagacg atagtgcctg
    26581 gccctcgcga ctcatcgcag acgacaagag gggtggcaaa agggtctagt tttcggtcga
    26641 ggcattcgac gaaagaatag aaggctttta aatcgatggc ggcgatgacg ggattcataa
    26701 ttatttggct tttatattat aaagcagaat atttttcatt atcagtcttg acataaaatg
    26761 tgatttgact tctcagaaat gttgaggaaa tatggttttc aaatgtagta taatgattct
    26821 ataaaaatac gcaggcggct tctttgtttt attcaacaag cgtaagatca aaaacatgaa
    26881 cggcgaacaa atagcaatta ttatcgtaaa tattctcagc ttcccctttt tcattgacgg
    26941 aataataatt ttgatgatta ttctatcgtt tctcctcggt ctggccaaaa agggatggca
    27001 ttcgctgtgg cgttttgttt ttgtgatttt ccttttggcg ggcgcagctc tctttgccgt
    27061 caaaccgctg gcgacctggc tggcgagcga tgcctttttc actttaatcg gctatcagcc
    27121 ggtcgtcgat tacggtggaa gtcacacgat aaacatcact tcatttaccg aactgattgt
    27181 ggtgatgggc cgcttgtcga ccgaccgcgc gaaattcacg gccgcttaca gtgctgaact
    27241 ggcattcaat attgccaaag ccgtcgcctg gtttgcaatt gtcctcgtcg tccataccat
    27301 ttcatgggtt gtctccgctc tcctttggcc gctcgtccgc ttaattattc ctgcgcgcat
    27361 ccgcaaaaag aaaatgcgcg ttctcggcgg tttgatcggc attgctcaaa cattagttat
    27421 cgtcgcttcg tatatgttcg ccacaagcgc catctcgcct ggttttacct atctttatag
    27481 cgctggtaat tatggtgcgt ttggcatcaa tgattatctc gtcggtatca ccgccggcct
    27541 caatcgcgaa aactcgtggg tctttagctg gctcgccctc gacgccaaag tctttcagta
    27601 caagattggc gaaacccact actttgtaaa ccttgagttt ttttatttaa gcgaaagttt
    27661 gatcaacgat gacttaaccg atgaggagaa agaagccatc atctccgctt ttgaagaaga
    27721 gtgcttcaat gaagaaggag aattcgtctg cacggtttga ttgtctcttg ataagagcat
    27781 tcattaaata tatactgatt tagcgtttgg ggttgtagct catctgggag agcgcgtcgt
    27841 tcgcaacgac gaggtagcgg gttcgatccc cgtcagctcc accactccgt tgaaattccc
    27901 cccatttatt ggaccaaaat ccggttgcaa taaaaggggg tttttatttt ttaacccatc
    27961 atatcgcgtt attattgata tatagactaa gtgagtaata tatatttgcg ctttttgaag
    28021 gaggagaata ggaagacaat tatcgtgacc ggtgcaaatg ccggaatagg aaaagcctgt
    28081 tctaagcagc tggctcaact gggtcaccgc gtcatcatgg cgtgccggaa tcagcaaaga
    28141 ggagaagcgg cgcgccaaga gataatatcg caaaccggga atcataaagt gatgctaaag
    28201 attgttgatt tgtcttctct ttcaagcctt aaaaaattcg ttgcagaaat cgctcaagaa
    28261 tacgagcgcc ttgacgttct aattaataat ggtgccgatt ttgatatttc gcgaagaaaa
    28321 aaatagtgac agaagatggc ctggaatcac aattcgcgac caatgttgtc gcaccttttt
    28381 ttctaagtct tggcttattg ccgatgctgg aaaaaagctc cgccggaaaa attatcaaca
    28441 tctcttcgca agggctcgtc atgtacccgt ttattaaact aaacatcagt gattttaact
    28501 gcgacaaaaa atacaacccg gccacgcaat attaccaaaa taaactggcg cttttaatgc
    28561 tctcacttta tatgaaagaa aacttcgccg gaaagacaaa aatagtcgcc attcgcgtca
    28621 ccaatgtcaa agttgacgtt aatcgctatc ccaatttgac tgattttcaa aaaaaggctc
    28681 tacaatttta aaagcaaatt cagtatatcg gctgaagaaa tggcaaaagc atatgtttcg
    28741 atggctgaag atgatagttt tactgggttc tatgtcgacg aaaaactacg cgaagtaaaa
    28801 gtgaacagag gcgccacaga ccataaaaga caaattttac tagttgatta tttgcaaaaa
    28861 atagccggac tggccgttgc taaatcataa agaaattctc tcaaaatttg acacaattaa
    28921 taatatttcg ttttttgatg caaaatatat ttcactttca agtgcttaga atataataaa
    28981 aatatctgtt acaaatttgc tgaaaggaaa caagacaatg aggaagatta ttctaatcgt
    29041 ttctggggct ctagctattc tagctattgt cattggtggt tcctggatat cggcttataa
    29101 cggcgcgatt gtccgcgaag ctgaagtcgt ggaagcacgc ggcaacgtcc acgccgcttt
    29161 agaggcacga tatgttaaag tcgacgtctt gattggcgcg attgaagacg cgaatgcaac
    29221 tattgaagga tatctgacca ttatagccga tgcgcgtgaa gcttttgctg ccgcgatcga
    29281 gaacggcaat gccgccgctg ccgatgaagc tgcggccctc gctgacggaa ccttcgtcac
    29341 tctcgtcgcc tacatggaag aaaatcctga tagttataat acagtcagtc tttattccgg
    29401 gtttatctct gagttcaatg cctcaacgaa tgttgtcaca ttcatgatta tcgagtataa
    29461 cggaactgtc accgactaca atacgcacat taaaacattc cccaatatca tcttcgttgg
    29521 cgggcgcgcc gtttataatt catacgaact gacaaactat aatcttgaat tgcctacttt
    29581 taaataataa ggtgggttat gactaaccgc caaacaaccg ccaggaaatg gttttttaaa
    29641 ggtctcgcct tttttctcat cgcctttcta acggcttgcc aaggcaaccc caagccgcat
    29701 attcgcccca ccgaggagtt ttatattaac gatcgttcca atgtccttct caattcgaca
    29761 aggtggacga tttatgctta tggcgaagag ctttatgaag acagccaagc gcaagagtat
    29821 aaagatcagg aaattagcgg cgctcagttt gtcgtcgcaa cttatgtggg acagcctgat
    29881 gacttttatc cacccgattt gtttaatagt tggggccccg gcgaaaacga catggggctc
    29941 ctcctcattc tttactttca acaaggagcc gaagaatacg aatttgacta tcaggtaatt
    30001 gattatgtgt ttggcgcgag aatgatgggc tttatttcgg cctttcgtat ggatgaactc
    30061 atctccgaat atttcaacga ccccgaaata tccccatccg attacgatca gcgcctgatt
    30121 tctttatatt tcggaataat gcaggaactt tatcttagtg tgtatgacta tgactcctac
    30181 aattatcaaa gtttcatgga tgaatatgct cttaataaat acgaagaatt cgatctattg
    30241 ccaagcgatt atgaaagaga tcctctccct gcctgggcct gggttttaat cgccgtcggc
    30301 atcctcttgc tcggcgtttt tccgggtcgc tacatccttc cctacgtctt ctcggcattt
    30361 accgggggaa aaggtggcgg cggacgcacg atcggctact ggtttcgtcg ttagaaggca
    30421 ccatctcgcc caggttctaa atgaaaatta atcttccccc tgattcgtta ccaaaactta
    30481 aaaaactaat ttccgttaaa gacgagaata tactggtttc cgagcttctt ttgcgttatt
    30541 tgctcaatag cgagaagtat ccgcttaaag cggaatttaa aaggcgctcg ccaaacgcat
    30601 cgacaaaaga gcagctatat gccgcaatgc ttgccgaaaa aatttcgccc aaacatcgcg
    30661 gaaagcagga tgttttgctt cgccatctca acggtcgatg cgcgcttttg gatggtgatt
    30721 actaccgcga taatcccttc tttaaaacaa ttaaaatttc agaatggagc atcggaaaat
    30781 ggcgcctggc ttttgatcgg tatgcgcctt atcaggcctt tgcgtttcgc gatatagatg
    30841 tctcctctca gggatatgaa gaaaagacgc acctcggctt ctttgaagag cctttttctt
    30901 ttcccgtgat catgcaagag gaccgggtgt ggatgagcgt cactcctcac gaaatcgaga
    30961 cgatgagaca agccgtcatc gaagctcgcg gcgtcgtcgc cgtgatgggt ttaggccttg
    31021 gttactatcc gtatatgatc gcccaaaaag atgcggtaaa aaaagtgatt gtctttgaaa
    31081 aagacaccga agtaatttcg ttattcacgc agcatctttt atcacagttc ccaaaccgcg
    31141 agaaaatcat tatcatcgaa gctgatgcgg tcaaggattt tgccaaatgt gttcaacaaa
    31201 cttatcccga ttatgtcttt accgatgtgt ggcagggtgt cgatgatggt ctgccgcttt
    31261 ttgtcgagat gaaaaaagag gagaaacact ttcccggcgc gcgctttcgc tactggatcg
    31321 aaaaatccct tttagccatg gttcgccgca tcatcatcac catttttgag gaagccatcg
    31381 aaggttttac gtccgaagat tatgagaatg ccgatactcc tctcgatgtt attttcagag
    31441 atatgtttaa caaattaggc aacgcgaagt tttcttcgtt tgatgacatt ttaaatgtat
    31501 tgaccgatga aggaatcagc gctttgctga gccgataatt tatagttata tgtatgccct
    31561 ttcaatgtag ttttaattat gtaaataagt aataattagt aaggcctaag ggatagacaa
    31621 aaacatgaag agcattcgcg aattatatcg aatcggacag ggaccatcca gctcccatac
    31681 gatgggaccg caaaaagcgg ccgcttttat tcgcgacaaa tatccgcgcg ccacttctta
    31741 cgatgtcgtt ttgtatggtt cgcttgcctt cactggacgc gggcacttaa ccgacaaagc
    31801 gatcatcaat acgctcgctc cgcgcccgtg catagtcacc tttgactata agagcaaagt
    31861 ggcccatgcc aacacctttg acatcaccat tcaccagccg gaccagacgg atatcaaaca
    31921 cagaattcta tctttgggcg gcggggccat ttcaatcgac ggcgaaacct tctttgaagt
    31981 cggagaaaaa tacgccgaaa aaacatttga cgaaataaaa aaactttgtc tcgccaacaa
    32041 ctggcgtttt tatgattatg tcgctgatcg cgaaggaccg gagatttgga attttcttgc
    32101 caaggtttgg aaacagatga aactgacgat tgaaaacggc ctcaacaacc gtggattttt
    32161 gccgggttca cttaaaattt atcggcgcgc cggcgatttg tataatgtct ttgatgaaaa
    32221 tgaaacattg gccgctcgtt cttatcggat gatgagcgcc tatgcctttg ccacgggcga
    32281 agaaaatgcc agcggtggca tcgtcgtgac agctccgaca tgtggctctt cgggaacgat
    32341 acctgctcta ctaaaataca tggcggaaca ataccaccat tccgatcagg aagttttaga
    32401 ggctcttgcc acggcggctt tgattggcaa cgtcatcaag cacaatgcct cgatcagcgg
    32461 ggcggaagct ggctgccagg ccgaaatcgg cacggcttgt tcgatggcgg ccgcggctta
    32521 tgccgagtta ttaaaactcg acttaaacca gattgaatca gcggccgaaa ttgctctgga
    32581 acataatctt ggcctcactt gtgaccccat cggaggctat gtccagattc cctgcatcga
    32641 aagaaacgcc gtagctgcct caaaggccat caccgcgacc cttttagcta aatacatcgc
    32701 cggaacgcac gccatctcgt ttgattccgt tgtcgcgaca atgctgaaaa ccggcaagga
    32761 catgcgcaag gcttatcgtg aaaccgccaa aggcggactt gctaatttat acaaaaattt
    32821 gaaaaagagc cgcgcgaagc gctaattgct caaagtcaac tcttgatgcg tgtgttaaaa
    32881 ttgaaaatat gaagattgcc atcatcggcg cgggagcctc ggggctcgtc gccgccatca
    32941 agtgcaaaga gaaattccct gaatacgagg tttttttgtt tgaccgcatg gatgccgtcg
    33001 cccgcaaagt aaaagcgacc ggaaacggta aatgcaatat cggcaacctc gattcttctt
    33061 ttgaacggta tcttcattca tcatttgtct ccagaatttt aaaagactac gaccaggaaa
    33121 caactttgct gtcgctagga attccaacga agacgatgta tggcagtggt ctatatcccg
    33181 tcagcgaatc ggcgagcaat gtcgtgactt tgctggaaga acgcgcccgc cttctgggcg
    33241 tcaacatcat tttgaatacg cggctgagcg aatattgcct cggagatgga aaaataaatc
    33301 ttttctttga taaatatcaa gccaaattcg ataaagtaat tttttccgtc ggtggtaagt
    33361 ccaatcccaa cctcggctca gacggaagtc tctttgaggt tttcagacgt cacagttacc
    33421 tagttgctga cctgacaccc ggcctttgcc caatacgcgt taaagaagat gttaaagcgt
    33481 tgtttggtca gcgctttcac acgatggcca gcctttttat cgacgggcag ttcgtccatg
    33541 aagaatatgg cgaagtgatg tttaaaaaag atggtctttc cggtattgtg atcatgaatt
    33601 tatcttcgat gatcgctcac aagaagacta atgatgccaa aatatatctc gctgctctga
    33661 atcagaaaaa tgagattgta accgccaaaa atctctttga gatcagtaaa aaaaaccata
    33721 accctctcct cgcctatgtc ggcgaagaac tcgccgccta tatatgcaaa accgccgata
    33781 tcagcgcaga tcacgaactt tcttatgaag aatgtcagaa attagcgaat gtcgccgccg
    33841 ctatcccatt tacttattta gaccgctatg acttctcttt ctcgcaagtc acaattggtg
    33901 gcatcgctat cgaaaactta agtgccgatt tatcatcgaa agtcgaagat ggtgtctatt
    33961 ttatcggcga agtcatcgat gtcgacggtc cctgtggagg ctacaatttg cggtgggcca
    34021 tcggatctgc attacacctc gttaatcatt tttagtctta attagaaaat aaaagccccg
    34081 catcaacggg gcgaggaaag aaaagttaaa gtcttactct tttttgtctt cgtaatgcac
    34141 gacgatttga ttgaacggaa tttggatacc gttacggtca aacaagagtt tcatctcttt
    34201 attcaaatcg cgggtgacct gatatttttt cagttcgtca gtccgggcat acatccgaat
    34261 gacgactgaa gaatcggata gggactggac tccgcgataa aaaggacctt cgacgatgtc
    34321 gggaattttg ctttttagtt cttcaatgtt gtttaaaata accttttcaa cacgttcgag
    34381 atcctcgtca taagcgatcg aaatatcgca gatggccggc gataagttgg ccgaggtatt
    34441 tatcgtctgc gaaggtcgga gttattgatg aaaaagacat cgccgttaac atcgacgagc
    34501 gtcgttgtgc gaacgccgat ttccgtcacc ttaccgcgga aactgtgcgc ttgaataata
    34561 tcgccgaccc ggaattgctt ttcaaagatg ataaacaatc cggcgatgac gtcttcgatg
    34621 aggctctgag cgccaaaact gagcgccaga ccgaggattc cggcaccggc gaggagggtt
    34681 ggcgtttgaa caccccaggc gcttaagacc agaaagacgc cgacgatgac gcttccatat
    34741 ttgaagatgc tgcgaatgat aatccaaccg tcgatttttt actgaagcgc cgaatgagca
    34801 aatcaatgac aattttaacg atttcgttga gcgcccacac aaagaagatg ataaccgccg
    34861 tctctaacag tgccatgccg ttattgacga acagattggc gacgttaaaa aacttgccga
    34921 ccgtgttgtt gacaaagacg ccaaatggtg tggttgataa ggcggggacg acggtgacga
    34981 ccacggtggc ggtcacggca aaggaagcca acatcttggc gatgagccga cgtctttttt
    35041 cgttttcgcg gattttttta atgctagcca tatttttcta taaagaaaag gccttctcat
    35101 ctcattttag atttgcgata ggcccatagc aataaaatat tgattagata aacgcttatt
    35161 atttcttttt ccagagcgcc tgatagcctt ctttttccag ttcttctttc ggaataaagc
    35221 gcaaggacgc cgagttaatg cagtagcgaa gaccgccttc cttcttcgga ccatcggtga
    35281 agacgtggcc aagatgaata ttgctcttct tcgttcggac ttcaatgcgg tggcggggca
    35341 aagaaaagtc gtcgcaatta actagaaggc catcttgaag cggccttgta aaactgggcc
    35401 agccggttcc ggagtcgaat ttatcatagg aggcaaaaag cggcgtgccg tcgacagcat
    35461 cgacataaat cccctcccta tgattgtccc agtattcatt gtcaaaaggc ggttcggtcg
    35521 cgttttcaaa gatgactttt ttctgaaagg ggctcagttc cggaattttt ttcataattt
    35581 tatcctcttg ataataatac cataaatcat aataaattat gataacgcta taaaaaaccc
    35641 acgaagacca tcgtgggtta gatatgtcaa aacgatatta ttcggttttt ggctgaattt
    35701 ctttcgtttc caaacgcttg gactcgccgt gtttgacaaa gaacatcgtg accgcggcgg
    35761 cgatggagaa aaagatggcg taagggaaga gcgtcagagt catgccaaaa gcatccatca
    35821 aaagaccgga gatgactggg gtaagcgcct gggccagcat gcttgcggcg tagtagtaac
    35881 cggtaaagac gccgatattt ggccctttcg acatttcgac gaccatcgga taggagttga
    35941 cgttaatcgc cgcccagcca aagccgacga cgaccatcgc ggcgtagata acccatccga
    36001 atgacaaatc gatgctggcg ataaaggcga ggatgaaacc aagaattaaa acgccgatac
    36061 cgacgagaat catgttgcgg cgtccaaact tggtggagag aatgccgatc ggataaaaag
    36121 cgatcaaggc ggcgccgaaa ccgatcatgg ccgggaatgt gtagttttcg atttgtaaaa
    36181 tattttgggc atatagcgac aatttgctcg aagccgcatt gtaggcgaag aaccagagga
    36241 agacggaaag aagaattaaa atgaaacttc tcctgacggc aggttccatc ttttcgccct
    36301 tggtgccttt ggcaaccttt tcgtcttctt cggtttcaag gccgagacga agcgattcgc
    36361 gacgcatctc atcggtccat ttgacttcct tgaccaagaa catgaagatg gccaagaaag
    36421 caagcatcaa accggcgaca atgccgaata agtagaggta attgaaagtg tcgtaatttg
    36481 acactttgac cgttccaaca accatgatta agacaaggga gataccgcct ccgatcgttc
    36541 ccatggcgtt gatgatggcg ttcgccttgg agcgaagtgg tttgatggtg acatcaggca
    36601 ttaaagaaac agccggcgtc ctgaacgaac acatcgcgat taaaacgatg aacaagatga
    36661 cgataaacga agcgaggtaa atccagtttg gctgcgtgat ttcggcgaaa atgtaggccg
    36721 ctcgaagttc ggccactttg tcggcgacct gagtgatata gacaaagcca taactattgc
    36781 cttcaagcag aatgatatct ttgatgttgc cacccaccgt aaagtaatag ccgatgacat
    36841 ccccgtttcc gttaagaatg gaaagcatcg gagagatctc ctcggcccgc acagcctgaa
    36901 attgatagaa atcgacgacg gccagcgaaa caaaaacgat ggcggccacg attgttccaa
    36961 ttaagacata aggtttccgc ttgcccattt ttgtgctggt tttatcagac cacgctccaa
    37021 aaagcggcag caaagccaga gccagcaaat tgtcgagagc cagcaggatg cctgaccaag
    37081 tctgattgag accaaagttg ttgacgagca cgatggacat gacgccatca taaatttgcc
    37141 agaacatgct gatgagtaaa aaagccaaac ccaccaaaaa cgtccgctta acgtttaatt
    37201 tcatgaatat ctccccgtgt gtttaacaca tacctaagta aaatattagc ataaacaaat
    37261 aatatttgta gaagaaagaa atggagtttt acggtcttat tgactcaaag atgtagtttg
    37321 agctctcttg gagggccaat tccctctttt gctcgctatc aaccgtccag gtgatgacgg
    37381 gcattccccg gcggcgaagg cggcgaagtt tttgataagg cacgcaatta atatcaaagg
    37441 cgatgaacgc aggtttcgag attaagcgga tattaagcga ccggaacatc caggcggcaa
    37501 acttatccaa accttccagg ctgagtgaga tcagctggcc ataaggatac aatttgggat
    37561 gattttggct cagccagcga atggcatagg gattaaacga ttcgatggcg atattgtttg
    37621 tctgcggata atcttttaaa agcgcgagaa tagatgctgg aagctcagga ttaaaggggc
    37681 catcaacttt catttcgatt aaaagcggca cgcgtccgtc aacaacttct aaaacctgat
    37741 tgagagtcgg aattttttcc ttggagagga gaagagatgt ctttgacaaa tcttctttcg
    37801 tcatctcgct gatgcggcgg ggaagaccag ccacgcgccg cagatcatca tcgtggacga
    37861 cggcgagcgt tccatccttc atcagatgca catcgagttc aattccgtag ccgctttcaa
    37921 ccgcggcttt aaaagcgcct agactgtttt cgtcataatt attatcgtgg agcccgcgat
    37981 gggcgagggg aatttttaga aagtttgtta aatcgtgcat atcatcattt cctttaacaa
    38041 ctattttacc tgttaaaagc ggcaatgtta ggactttttt agccgatgtt ttatcgtatg
    38101 caaaataaac tcgctgatct ctttgggcac atacggggaa atatcgttac caaagagggc
    38161 taattctttt attgccgaag atgtaacaaa taagttgtta tgcgaaggaa acatgaccag
    38221 cgtctcaatc tccgggtcga tcgtgtgatt aaactgataa agggtaattt cggcttggta
    38281 gtcattcgta ttacgcacgc cgcggataat agaggcgttg ccttttttct gagcgtaact
    38341 gacgactaat tcttcgctag cttcaatgac gacattggcg atatccgcgg tcgccgcttt
    38401 aagcatatcg acccgctcct cggccgtgaa taggtattgc ttgttgggat tgacggagac
    38461 aaggacataa aggcgggcaa aaatcttcgc ggcgcggcga atgatatcga gatgcccatt
    38521 gcttaccgga tcaaaagaac ccggatatac agcctgtctc atcctttaaa aaactgcagg
    38581 acatcggcgg cgaattggtc ggggatgtcg gtgatcgcgg tatgcgaaac gcctgaataa
    38641 acttttttgg tggcattctt aagggcggcg agagtctcat cgaccatata ttcgaggacg
    38701 accaagtctt tgtcgcccca taaaaggagg acggggcatg ccacttgtgc gatatcgttt
    38761 gtgcctccat tataaagatt cgaaaagttg gacatattta aattggcgag gctccagtcg
    38821 atgtcgatga gattgcgttg ttttaatgtt tcatcaagca ggtaagaaag gagttcgggc
    38881 tcgggtttct tgacgttgta gatcgttaag gaatagatgt aatcataaaa ggctttgttt
    38941 tgcgtcgccg tggcatagag ggccggagcg acttgaacgg gatctttggc catctcttcc
    39001 ttgctggaat atatttggcc gattaacggc atgttgtgat cacccttttt aaaaatcgga
    39061 tagccgcggt aagaggctcc cgaaagggag atgatcttct tgacaagcga gggttttttg
    39121 gccgctaatt tgtaggcgac tccggctccg gttgaccacc ccaaaacatc gactttatga
    39181 acgccgagaa tgctgagaaa gtctatgaca tcatcggcca gttcatcgag tgaatcaaag
    39241 cgcgcgttgt atgaggaatc gccaaaaccg cgcagatcgg gggctaaaac gcggatatcg
    39301 tgaggaatcc gcttcaataa ttcgtaaaaa taggcgctgc tcgacatgtt tccgtgaatg
    39361 agcaaaaaga ctttttcgcc ttgaccttgc tcgatgtaag cgtattgctc accgtttttt
    39421 agggtgaggt gttttttgac aaatcgttcc atatttttcc tcctaaataa agtatttgtc
    39481 ggtgctagaa ttcgtgaatc cgagaacaag agagacaaag atgtgcggct tttcatacat
    39541 aaaagcgtgg ccggctttcg gaacgatgat gtgcgtggaa tttttaatgt gctcggcaag
    39601 ataagcctgt tcgcttgacg gcgtcagttg atcgaattca gacgagacga taagtgtcgg
    39661 cacttcaatc cgatgaatgt tggcggcctc gtcgagcgat tcggcggagg tgaccaaacg
    39721 cttcattctc tcctgaaatt cgggagtcga aaaaagagga atgagaagct tgcggcgatg
    39781 ctccatccat ccatagttgg cgacgtagaa gttcgaggaa tagatgatgg gtatcgtcat
    39841 attataatag gcctcgccgt cgagagtcgc tccgacctta ttccagcccc ggccgatgtc
    39901 gcggagccat tcatttgttc gcgatgtcgt gttaaagagc attaagcgcc ggagcaaatt
    39961 ctgatatttg agagcgaatt taatcgccac ctcgccacca tagctaatcc ccatgatgtt
    40021 tatctttgcg atttgcagat ggtccaaaaa agccttgacg acctcgacct gaatgtcctg
    40081 agtataatgc tcatccatgc gagctgattg gcccatgtcg agaagatcga tgcgcaaaaa
    40141 gcgattgccc ttgcttaatg ccgggacaaa agtcttccac gaaacggtcg acatcatgat
    40201 gccgtttaaa aagagaatcg gctcgcctga gccatcatct tcatagaaga tttgcttgtt
    40261 atgaaaagta aagaaaggca ttttacgctc cgatgatgcc gagggcaatg gcttcctggc
    40321 ggaggctatc gcggaatttg ggatgggcga tcgaaataat cttttcgacg cgctcctcaa
    40381 tcgttgtgcc gcggagattg acgatgccgt attcggtgca gagatagtgc aaatcgttgc
    40441 gcgataaaga gacggccgcg cccggtttga gctggcagac aatcttggat gtttcttcgc
    40501 gttctccggt ttccggattg cggacattgg ccgtcgaata taaagcgatg atcgagcgcc
    40561 cgtttttact gttttgggcg ccgatcgcgg tgtcgctctg cccgcctgtt ccgctgaatt
    40621 gcttcgaacc gatcgattcc gagcagcact gcccggtgac atcgacttca agcgtcgtgt
    40681 tgatcgaaac ctgattgtca ttgttggcga tgacataggg atcgttgacg taagagcccg
    40741 ccagcagcat gacggccgga ttgttatcga taaagttgta taaatcgcgg cttcccatcg
    40801 caaaggtggc gaccatctta cctgggaata gttgtttgtg ttttccggtg atgactccgg
    40861 ctttggctaa ttttgccatc tcgttggtca gcatttcggt gtgaacgccg agatcctttt
    40921 tgccgtaaag cgactttgca acggcatttg gaatgccgcc gatacctagt tgaatgcaat
    40981 cgccatcatt gattaattcg gaaatatatt ttccgatcaa gaggtctttt tcgttgggtt
    41041 cggtatcggg tatttcggga atcggatagt tcacctcgat gaaataatcg acatcgctga
    41101 cgtggacttc caaatcgccg aaagtgcgcg gggcattagg atttatttcc aagatgacaa
    41161 gatcggcttc cttgataact tgtttttcgt aggtgttgcc aagcgaaagg gaaacatagc
    41221 cgtgctcgtc ctgaggcgag gcaatgccga cataaatgtt aggtttaatg tgatccaggc
    41281 gctttgtgcc ggcatagtgg agcacgttgg gaataaagga gacgttgccg ttagcgtggg
    41341 ctttgcgcat tccggcattg tagaaccagc cagccagttt gaaagtatcc ttgtaggccg
    41401 gattcatata aaattcgtag tcttgcatcg gcaggcagtt ggtgacggtg acatttttga
    41461 ccaatccgtg caaagtgtgg agtttgctta aaaactcgcg tccttcgctg gcgcccaagc
    41521 cggtaaaaat atgatcattg ctcttgacga gtttcaacgc ctcttcaacg gtaatgattt
    41581 ttttggaaag atcgtgcttc ataggtttct cctttatgtt ttaacgtgcc ttgcggcccg
    41641 ccgcaacgcg caattgcggc ggggaacaca aggtatctga atgaattgac taagcctttt
    41701 tggcggccgg gacgagagcc cagacatagt ctaaggttct cagggtgtcg acttcggcca
    41761 aagcgctcgt gttgactgga tcagcgagat agagcttgct gaaaccaaga tcggtgacgc
    41821 ctaggcgttt gccatcagcg taatcgattt cactgcccat tggaagatcg actggttctt
    41881 cttccatggc attgatgtaa ttgagccatg tgagttcttt gtcggcggcc ttgactctgg
    41941 ccagaccttc taagaagatt ttggcagcga cataaccggc gatggcgtat gaactacctg
    42001 aataagcgac ttgtccgccg gtgttcatct cggtcagata agcgacatat tccggagtca
    42061 aagcataggg gttaagtgtc gcatcatagt aagcgaggtc ggtgacgtca agccatgaat
    42121 tcgtgaagac cgggcgatcg gcggtgattg aaccggcggc ggcggcagct ctgagaatgt
    42181 cagtgttggc cgtgacataa gaggtgatga ctttggtatt gaagttcgcg tctctcatgt
    42241 aattgagaat ttcggtgaac ggaacttggt tggcggcgac gatgacgaca tcacatccgg
    42301 cggtctttaa agtgttgacc ggtgcggagt ggttcgtgcc ttgttcggcc ggggtggtga
    42361 catagacgag gttttctttg gcggcgacag agaggagttc agcttgtttc tgaacgccgt
    42421 agagcatgcc ttcgccggca tcgtcggtcg tcgaaataac gccgattttg gtggcattta
    42481 agccgctgac agcgtcatcg gcgagagctc tggcgagcat catacgacct tcggagttgt
    42541 agatgggctg aacgggcatg acggccttat taaagccttc ggctgcttct tgatataaat
    42601 cggaaatgcc ggtagcggca tagaccatcg gaatgccttg atctttgata tagtcgacag
    42661 tggcggcgac ggtgttggtg ccgaagtgtc cgacgagagc aaagactttg tcttgttcga
    42721 ctaatttttg cgtggccgct aagccgatcg cgccgtcaaa tgagtcatca tagtgaacca
    42781 gttcgacagt ggcgccatcg aatccgccgg cgtcgttaaa gtttttcaag acggcgttga
    42841 tgccggcatt gaagggaacg ccgactgtcg caaaagcgcc agaggtggcg gcggtgttgc
    42901 cgatgagaat tttttcagcc gtgacacctt ggacttcagt gccatcgtat tgcttgacga
    42961 cttcgacggc aggattacag gcggctaaaa tgacggctga aacggcagag agaacaaata
    43021 aattgatttt tttcattaat gaaactcctt tcattcattt gtctattgag aaccgtgatg
    43081 attgctgatc gactcgcaga cgtaagaatc gatgagttct tgcatccgat aggaaggagc
    43141 tatcttgatg cctatgcggg aggagattcg gatggaggcg aaggttaagc taatttggtt
    43201 ctccggaatc gctatcttat cgcttctttt ccatagcctt gtatttcgat taagaatatt
    43261 tgaggcgtgg tgttgatttg tcggtgtaca cccgttgcgc gcttacaaat cttacttttt
    43321 ttctccacct cctaaataag catcgatgag cctctgatcg ttgattaatt cgctcgcttt
    43381 cccggagatt gatacattgc ccagttcgag aacataggcg aagtcggcaa tcttcagcgt
    43441 agccagagcg ttttgctcga cgatgaggat ggtagttcct tgttttttaa tttcgacaag
    43501 ggtattaaag atgtcctgga tgatgagcgg ggccaagccg agcgatggtt cgtcgaggac
    43561 gagtatcttc ggcgaattca tcagggcccg ggcgaccgcg agcatttgct gctcaccgcc
    43621 actcagggtg ctggcctgct gtctctttct ttccgcaagc actgggaaga gcttgtagac
    43681 ccgatcaaag ttttccttca actgcgcttt gacgctgatt gagactttcc gctttattcc
    43741 atgttcatct tcaattaatt gggtatggcg tttcaagcca tatgcaccgg cgagcaggtt
    43801 ttcttcgacg gttaatccgg taaagatctg ccgcccctcc ggcgacagag acaagcccat
    43861 ctttgaaacg gcgtgggccc gtttgccggc gacgttagcg ccgttgagca taatcgtcga
    43921 tccggaaacc ggtttaacga gtcccgagac ggccttgaga atagttgatt tgccggcacc
    43981 gttagcaccc agcaaagcga ccatcgcccc ctgttcgaca tcgatgttga tgccgtggag
    44041 ggcctcgatg acgccatagc ggacgacgag gttgcgaatt tctagtaatt tcatctttca
    44101 acctcctcgg gggcgcctag ataggcggcc tgaacatcct tgttggactg aatctctttg
    44161 gcgcttcctt gagccagttt tttgccgaag gaaatggcca ggatattgtc gcagacatcc
    44221 atcaccagtc ccatgtcgtg ttcgacgagc agaatggtgc atttgtattc atcgcggatg
    44281 cggcgaatga gattggcgag ttcgacggtt tcgctgtcgt ttaagccggc ggccggttca
    44341 tcgaggatga tgaggtgggg atcagccatc agcgtcctgg cgatctcaat cttttttaag
    44401 acgccataag gaagacccca tgcgtaccaa tcgcgataaa gagataggcc cataaaggct
    44461 aaaactttat cagctcgggc gcgaatcact ttttcctcga ggcgcaggat cggcaaatgc
    44521 agcatgtgaa taaagaaatt agactgatac tggcggtgag cggcgatcag gaggttgtcg
    44581 agaacgctga cttctttcac cacttcaagg ttttgaaaag tccgcacgat gccttgaaga
    44641 atgacattgt ggacggccag tttattaagg ttgacgactt catcgaggcg gttgcgcaaa
    44701 tagaggtgtc cgctcgtcgg tttattaaat tgcgtgatgc agttaaaaac agttgtcttg
    44761 cccgcgccgt tagggccgat gagcccgaat atttcgcctt gcttgacatc aaaagtcaag
    44821 tcgttgacgg ctttgacgcc gccgaagaac atgcacaggt tttcggctct gaggatgacc
    44881 ccctcgggta gcgcgtaact gtcctgctga attctttcca gttgaacttt aagcttagct
    44941 tggaagttat tgagttttcc ttccaattta gcgattcgcg ctttgtttga acgcacgaac
    45001 ttcgctcgtt tagcctcgcg ttttgcattt acggccgcgg tatatgtttc gttatcagcg
    45061 gtgaggcgcg actttaatgc ctcgagttct tgggcggttg gtttggcttt gctaaggtcg
    45121 cgctcaagcg atttttcaaa cgcgatgtgg cgctttgcct tatatgcttc cagctcttcc
    45181 gccaaggaag cctcaaaaga ggcaagattt tgatcgatga gtttaatttg ttcgccgctc
    45241 ttgtcttcct ctgccagctt ggcgatggct cgcctcgctt tttcgcgttc gatacgatag
    45301 cgcatgcgaa gtttcttctg ataagcggcg atttcgagtt tcgaagttcc ctcaaaaacc
    45361 tctttcttat cggatgcctc ataatcagcg gttgcataga ggcgcgcatt tttggcgatg
    45421 cgcagttcaa ttttgcggtt cgcggcatcg tcgatgtctt taagatgttc gacttcgaag
    45481 tcggcatata atgaggtccg cgacttataa aaggtgacgc gctgagtcaa aaagtcgaca
    45541 tacttgccca gaagataagc cattacttta ttcttcaatg ccataacggt atatcctcca
    45601 tttcttggtc atttttttaa gccataagcg gaaggtaaaa agcatttggg caaagccgcc
    45661 cggataaaac atgacgacaa ggataatcaa aacgccggtc accatcatca tgaaagcggg
    45721 gttttcgttg aaaaaagcga tgttttgaag gaatatcgac tggaatccgt atatgacaaa
    45781 ggttccgact aatgtccccc acagcgattt tgcgccgccg atgatgaccg cgccgagaat
    45841 attaagcgac atgctaagag tcagaatcgt cgccgtcgtc ggtgagattc ccgggagata
    45901 ggcgtgatag aaaaagccgg caatcccggc gaaaagagtc gagatgatga aagccagaag
    45961 gcgatatttg agcaaggaga tacccatggc ttgggcggcg gcgaccgacg atttcatggc
    46021 caccatggcc cgtccggtcg gcgaattgat gaggttggca gtcccgacca aaagcatgac
    46081 caagacgacg ataatcgtga agtaaacggc attatttgaa agcggtattc cgaagaaatt
    46141 aaggttgacg ccgcgaatta tcaaggtgct gcccttaatc gtcttaagca gtttgacgat
    46201 gatttccgaa agagccaggg tcatgatggc cagataaatg ccttcgatgc gcagggacac
    46261 aaagccgacg acaaagccga tgacaatcga agcgattccg gcgattaaaa aggcgggcag
    46321 aatactgatc tgccattcct ggaggccgta gtggcaaaga tagatgccga ggcccataaa
    46381 gcctgccgtc cccagcgagg cgaggccgga gtagccgagc aacaggcaga agccgatggc
    46441 ggcgatggaa tagatgctga cgaacgcgat tgtcgtcatg atgctgcgcg ggacgatgcc
    46501 ggcgatatta agaagctgcg tgattgctaa aagcaaaccg aggaggccga attggcccag
    46561 cggatttgac atgatgcgct tggcgtgggc tttccagccg ctttcagggc gcggcgaggt
    46621 gtgggccaga tattcgtttt tgaagcgatt gttcatggta tacacctcat actttcttgg
    46681 caaccacttt gccaaataaa ccttgtggtt taataagaat cgcaattaga agtaaaatgt
    46741 agacgatgac ttccgcccag ctctggaaaa agggatttat gaatccgagc cagccgacga
    46801 cggaaattgc caaaggaatg atgatggcgc cgagaatcgg ccccaagaag gtcgagaatc
    46861 cgccgagaat tccggctaaa aaggaactga cctgaatctt cgtcataaac gatggcgtta
    46921 acgaggcgtt gccggcatta agcatgacgg ctgccaagac ggctaaagcc gcggcgatcg
    46981 cccacgaggt ggcggtgatg acataagtat tgaccccgac gatgccggcc gtatattcgc
    47041 ttgaagccgt aacgcggacg ccgagacccc atttactttt aaaaagtaag acgaataaaa
    47101 cggttaagac ggccgctgtt atggcgatgc ataccaccga atgtttggtg atggacagtt
    47161 cgccaccgag catgctgatt gaaaaattac catcgataaa agccggtatt tgcggcggaa
    47221 tttgagctcc gaaaataagc ggaaccgctc cgaagattat cgagaccaag cccatcgtga
    47281 taatttgttt tccgacgatg ttgatatttt tgccgcgccg aaaaatcatg atatcgatga
    47341 atagtccaaa ggcgataccg aagaccaagc cgatcaagat ggcgaggatg taatggacgc
    47401 tgtactgcat ggcgagatag gcgacgacat aagttccggc ggcggcgatg gcaccctgcg
    47461 caaagttggt cgtattactc gtgcgaaaaa tcagggtgat acccatcgtc gcgaccattg
    47521 tcacgcagat ggtcaaaagc gatccggtta tcgtatttaa taaggacagg agttgggcca
    47581 tagtttatcc ccagcggatg atgttggcac tccacacata gccgataccg gccgcgatca
    47641 tgcaaacaac atccccatct ttcacttttc cttctttaag cgccaattcc agcgagagaa
    47701 tttggtcaat ttggccgatg tggccatagt cttcaagata gatcgtctga tcttcgcgaa
    47761 gaccgagatc ggcaatcatt gccgcgtgac ctgagcgctt gatgtggagg atatccagga
    47821 atgtgatgtc ttttcttgtc agttttgctt gctgtaacga ttcatcgatg caacgatacc
    47881 agtttgacat tgagacttcg ttgagtcgat ctttcatctt tttggcgtcc aggaggcgaa
    47941 gagatttata accttcttcg atattatcgg cgttaaacgg cttggcaatg ccgccgattt
    48001 cgacgccggc tgttcttgaa agcgaaccat cggcgatgat tttactgccg agaagcaggt
    48061 ttttgtcatg gtcgcgcttt aagatgatgg cgcctccgcc agcgcccaaa ttatacatca
    48121 tcgacatatc cttatcggca tagtcgacaa agtcgccgtt gcggtatccg ccgacgatca
    48181 taatcgtgtc gatgctttta tcggccacga gcatatcatg cgcgattttc atcgccgaaa
    48241 cacatgtgca gcagcggttt tgaacatcga tgccccacgc gttaatggcg ccgatgcggt
    48301 cttgaatata aagggccgag gtagttaaag ggtattcctt ccactcctct ccgatgcata
    48361 agataacatc gatatcgaga ggatcgatgc cggtattttt taagcagttg agagcggctt
    48421 tagcacccat ttcctgcgtg ccgtcatcgg gtccgggaat gtatttctgg cggatgccga
    48481 gcttattgat aactgcctct tcgctccaga cgccattcgt cttagcggcg atttctttgg
    48541 ctgtcatcac ttggctagga agatacgttc cgacgccgac gatgccgact ttgacttttt
    48601 ccatagcctc ttccttacag tctcatgccg ccgttaactt caaggacctg gccggtcaca
    48661 taacttgctt catcgctggc caagtagaga gccgcatcgg cgatttcatg aggctgaccc
    48721 aggcgtttta acatagttag ctcggcaaat ttcttcaata aatcttcagg gacggttttc
    48781 atcatgtcgg tcatgatgta gccgggagcg atggcattgc agcgcacatt ggcccctttg
    48841 cgggcgaatt ctttggccca gcttttggtc atgccgatga cgccggcttt gctggctgca
    48901 tagttggctt gtccgatgtt gccgtagacg ccgacgactg acgagatatt gataatgctt
    48961 ccatatccgg cttcttccat cttcggtcca aaaacgcgcg tcatgttaaa gacacccttg
    49021 aggttaaccg agatgacgag gtcaaattgc tcatcggtca tttttctggt catggcgtcg
    49081 cgagtgatgc cggcattgtt gacgagaatg tcggcttttc catatttggc aaacacgtat
    49141 tcgccaaatc ttgcgacatc atcgctatta gcgacgttta atttgtaaaa ctcgacatca
    49201 ggatggctgt aggtgagatc catcatatcg acggcgatga ccttcgctcc gtgtttgacg
    49261 aagagttcgc tcatcgcctg tcctaatcct ttagccccgc cggtgacgat ggccacttta
    49321 ttctgcagtc tcatttttca ttcctccttt attgataaaa aatgattata ttttcttaaa
    49381 gatgatggct gtgcccatcc cgccaccgat gcaaagagag gcgagcccga gtttgaaatc
    49441 gtgtctttgc atgatatgga gcagcgtgac gctgattcgg gctccggaag cgccgacggg
    49501 gtggccgagg gcaatcgcac cgccctgaag attggtcttg gccagcaact cgtcaaaacc
    49561 gatgtcgtgt tcttcagcaa gactcttgag gacgccgaga ctctgcgcgg caaatgcctc
    49621 gttcagctcg atgacatcca tgtcttttaa tttatatccg cttcttttca taacatcgcg
    49681 aatggcggcg acaggcccga ggcccatata ttggggatcg acgccgccct ggccgatgcc
    49741 gacgatttca gctagtggtt taagatgatg cttttgaaca gcttcctcac tcatcagcat
    49801 taaaaatgct gctccgtcgt tgatacccga tgagtttccg gccgtgacgc ttccgtcttt
    49861 aacaaaagcg gggcgaagag cggccagttt ttccaaattc gtcgtccgat taggatactc
    49921 atcgcggtta aagatgatgt cgcctttacg gtctttgatg gtgataggaa cgatttcgtc
    49981 atcgaacaag cccgcatcga cagctttgat ggccttgact tgcgaggcat aggcaaactg
    50041 atcttgttcc tgacggctca aagcgaattt tacggcgatg ttttcagccg tgacgcccat
    50101 gtggacttgc gaataggcat cggtcagagc atcgtagatc atatgatcgg taagtttaac
    50161 atcgcccatt ttaatgccac cgcgaacggc agacgggact aagtgcggag cgcgagtcat
    50221 agactcgatg ccgccggcga tgacgatttc atcaaagccg tcgcgaatgg cggtgtagcc
    50281 cgtcaggacg gatttcatcc ccgaaccgca gagcatgtta atcgaataag ccggtacttc
    50341 aaccggtatg ccggctttga ttgagacttg acgtccggga ccttggccga ggccggccga
    50401 aaggacattg ccgacgatta cttcgctgac atccgtcccg gcaactttgg tcgcgctgag
    50461 caaggccttg acgacttcgg cgccgaaatc ggtcgcatca attgttttga gcgatccgag
    50521 cattgaaccg gtggcagttc ttttcgcggc aactacatag acttttttca tacttttctg
    50581 ttctccttaa agtgcattct tttcaatttc ttcttttaga agcggcaaaa tttgattgat
    50641 gtcgccgacg atgccgagat cagctactga gaagatcgaa gcggtccggt ctttattgac
    50701 ggcgatgatg tagtcgcttt cttccatgcc ggccaggtgc tgaacggcac cggagatgcc
    50761 gagtgccaga tagagttcag ggcgcaccgt tttgccggtc tgccccactt gccgatcggg
    50821 attaattttt ccgcagtcaa ctagcgatcg tgagcaggaa tatgttccct tgattagtcc
    50881 ggcgagatat tgggcggttt tgagattctc gtcaccgccg attccgcggc cgattgagat
    50941 gagaattttt gcttcttcga tattggcaat ctcttgggtg gccaccactt cttcaagtac
    51001 ctcaacgcgc ggcttatcaa ctgcgacatc gagatggatg acatttacca gtcggccctt
    51061 gttagcgccg atgcgcgcca tgacgcccgg tcggacagtc gacatctgcg ggcggtgttc
    51121 ggggcagatg atggtagcaa aaagattccc gccaaaagcc ggacgcgtca tgaaaagaga
    51181 accgtttacg tcgatttcga gtttggtgca gtcggcggtt aagccggtgc cgaggcgggc
    51241 agagaggcgc ggggctaaat cgcgaccgat ggaggtcgcc cctaaaagga tgacattcgg
    51301 attcacggtg gtgatgattt tttcaaaagc cctggcatag gcatcagtta aatagtattt
    51361 aagatttgaa ttatcgaccg aatagataag atcgatgtcg aaagttttca agtcctcgat
    51421 gagcccggac aaattgctgc cgacgatgcc ggcgacgaca tgccctccgg agacatcggc
    51481 taaatcgcgg gcggccccga ggagttccag cgatactttt tgaatcttgc catcgcggac
    51541 ttcaataaaa gttagaatgt ctttgttcac cgtgtggccc tcctagatga tgtggcgctt
    51601 cttcatcgta tcgatgatgg ccttaacggc ctcgcgtggt tcgacttcga gaagattctt
    51661 ttgcttatcg acaactttgt tccaagtcga tttgactttg gtcggcgatc cttttaaacc
    51721 gagttttgtg gtgtcgacga tgatgtcgga tgctcctttt aattcgacgc tttcggtctc
    51781 cgtggcttcg atgagtcggc cgacatgaag cgagcgcggt ttggcggcat cgcccaagat
    51841 tgtcaccagc aagggagcgg tggcttttaa ggtttgatag cgatcttcaa attggcggac
    51901 ggcgataaaa taaccgtcct cgaacttgag actattaaca taagagattt gcggaatatc
    51961 aagatgctcg gcaatctgcg gcccgacttg agcagtatca ccatcgatgg cctggcggcc
    52021 ggtgatgatt aagtcgtatt cgagcgtctt taataaaccg gcaagagtca gggaagtggc
    52081 tagggaatcg cttccggcaa aaagccgatc ggtcaggagc atggcgcggt cagcccccat
    52141 tgccagggct tcataaagta cttttttagc ctggggtgga cccatcgaaa cgacggtgat
    52201 gtgagcttga tgggcgtctt tgaggcaaag ggcgaattcg agtccgactt tgtcatcggg
    52261 attcatgatt gaaacggcgc cttcgcgatt gagggtaccg gtgacgggat cgattttgat
    52321 ctcggtcgtg ttcggaactt gtttgataca gacgattatt ttcatcttta ttacctcaaa
    52381 accgctgcgg aaataaccat tctttgcact tccgaggttc cttcgtagat ttccgtgatt
    52441 ttagcatcgc gcatcatgcg ctcaacagcg tattctcgag tgtaaccgta ccccccatgt
    52501 aactgaacgg ccttattggt aaccatcatc gccgtttcag aggagaagag tttggccatg
    52561 gccgcgtctt ttgataaggt tttggctttt tgctcttttg aggtaatcgc ccgctcgact
    52621 aatagacggg cggcgtcgat atgggtggcc atgtcggcga tctgaaattg cgtattttga
    52681 aaagcggaga gcggacgccc gaattgcttt cgctcctgaa catagttcac cgtctcacgg
    52741 agggcgcctt cagcgatgcc gagggcttgg gcagcgatgc cgatgcggcc gccgtcaagc
    52801 gttttcatcg ccactttaaa accttcgccg acgggaccga ggatattcgc ctttggcacg
    52861 cggacatttt gcatgatcaa ttcgcaagtc gaagaaccgc gaatgcccat cttagcttct
    52921 tttttaccga cgctgaagcc gggcatgtcg gcttcgacga taaaagcggt gatgcctttg
    52981 atacccttgc tcttgtcggt catggcaaag atgagataga catgggcata accggcgttg
    53041 gtgataaata tttttgttcc gttgagaatg taggagtcgc catcttcaat ggctaccgtc
    53101 tgctgattag aagcatccgt gccggcgttt ggttcggtga gaccaaaggc gccgagccat
    53161 tctccattag ctaactttgg gagatatttt tgcttttgct cttcgcttcc gagctcggct
    53221 atgggagcgg tgcaaagcga gacgtgcgcc gacaagatgg cgccggtcgt cgcacaatac
    53281 gaggatattt cgcgaacggc gttaatatac attttataat cgccgccggc accgccatag
    53341 gctttgggga tgatgatgcc gaggagaccg gctttaccca tcttttcaat ggtttcgctc
    53401 ggaaagcgct cttcttcatc gatttgcgtg gctaaaggtt tgacttgctt gagggcaaat
    53461 tctttgatga agtcattaaa tagttcgtat ctcaaaattg tgtccataaa ttacctcacg
    53521 aggatggtgg cggtgccggt gattacgctc ttgccgtctt gattgatggc ggtcgtcgcc
    53581 agtttaacga ttcttttatc ttcgcgaagt tcgataactt caacttcggc ggtgatcgtg
    53641 tcgtttaaga agaccggggc gacaaaccgc aaatcctgac cgagatagat gctgccttca
    53701 ccggggaggc gggtccccaa aacggccgag aataaactcg ccactaacat accgtgggcc
    53761 acgcgatgat gaaaaacgct tttttgcgcg acttcttcat taatatgaag cggattataa
    53821 tcagttgata attgggcaaa agcatcgact tgctcagcgg tgattcgctt gctgatcgaa
    53881 gctttcattc ctcgttttaa ttcttcaaat ctcacgggat atcctccaag taatgatggg
    53941 tagtggcgca gggttatcgg aaaacggagc aaatggcaaa caatgattat atgttgaaaa
    54001 ctggatgagc caattttata aattgagaca aatatgaatg ggtattcatg tttatattgt
    54061 aagcacttac ataataattg tcaattagtt tgatgctcct ttttgacaaa tgtcgcattc
    54121 aaaaatgctg tgggataaaa aatcaggcga tggtagaata tgaataggcg ttcaagaaca
    54181 gatatagaaa agatatggaa tcatcggtct tttatcccaa agcaaagcgt ggaaaagcaa
    54241 cttttcggcg cattatcacg gctgccacca aagtctttta tgaacgcggc tatgcaaatt
    54301 caacaattgc tgatatcgcc gcggaagccg aagtcgccgt cggcacgatt tatatttatt
    54361 tcaaagacaa atacacaatt tattaccaag ttttaaaaga ttttcaaaat cagattcgcg
    54421 aacatttaaa tcgcaatatc aaagaagctc atacgcggcg cgaaaaagaa agagccggca
    54481 ttcgcgcctg gcttgaattc gtcctggaac accgtcgggc ctatgagatg atttgggaat
    54541 cactatatat cgatcgccgc ctctttgacg actactattt gagtttctcc gaggcttatc
    54601 gcaaaggctt ggaacacgac tccaaagaat tggtaaatgt cgattttgag acggtttcgt
    54661 atatcctgat gggcatcgcc aacttcgtcg gcattcaagc gatgctgaca gccgatatcg
    54721 atgatcagaa actcgatgcg attgtcgatt ccgtcatgct gatcctggcc aacggcctct
    54781 ttaaaaagca atcttgagat tatttgtctc cctttctaca gaaaaaaacc aagattatca
    54841 attcgacaaa agaagtccta ttcttggatt tattgccttg cattataatt attaatacgc
    54901 gaacaagtat ataaaggaga aacatatgtc aaaagaaatg tgggcttatc tcatcgccct
    54961 cgccgtctct ttgctcgcca tcggcgtcgc cgtctggatg gctcgctggg tgaagaaact
    55021 aaaatccgaa aatgcaaaaa ttgcctatgt ttcaagcttg attaagagcg gtgctaacac
    55081 cttcttaaag cgggagtata ccatcttggc cgtctttgcc agtatcctct tcgttttcat
    55141 tctcatcttt ttacccaagc cgatttggca agtcggcgat gaagtcggcg tcaatcgcgt
    55201 cctccctaat ttggcgatgg ctctttctta tctcatcgga accattttct ccggtatcgc
    55261 cggaaaaatc ggcatcgaca tcgcaaccat cgccaacgtc aagtcagccg aagccgccac
    55321 caaaggaata aaaccatctt ttatggccgg ttttcgcggc ggagccgtca tgggcatggc
    55381 cgttgtcgcc agctgtttgc tcggcgtcac attagtcttc tgggccactt acggttggac
    55441 cggcaatgcc cgcgtcttaa tcgggtttag ttttggcgcc agctcactcg ccttatttgc
    55501 caaagccgga ggcggtattt tcaccaagac cgccgatatc agtgccgatc tcgtcggcaa
    55561 agtcgaattg agcatccccg aagatgatcc tcgcaatccg gccgtcatcg ctgataatgt
    55621 cggcgataac gtcggtgatg tcgccggaat gggtgccgac ctctttgatt ccaatgtcgc
    55681 ctcactggcg gcggctttag ttttggccat cgccttagat
//
LOCUS       DAOSSL010000003        54477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_665_length_54477_cov_23.006835, whole genome shotgun sequence.
ACCESSION   DAOSSL010000003 DAOSSL010000000
VERSION     DAOSSL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 54477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54477
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_665_length_54477_cov_23.006835"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..292)
                     /gene="rpsR"
                     /locus_tag="PLH17_00705"
     CDS             complement(65..292)
                     /gene="rpsR"
                     /locus_tag="PLH17_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOR20333.1"
                     /translation="MGYKKVRPRKKVCYFTKNKITHIDYKDVELLSRFITPNGKISPR
                     RSTGTAARYQRELAKAIKNARFMALLPYVID"
     gene            complement(305..730)
                     /gene="ssb"
                     /locus_tag="PLH17_00710"
     CDS             complement(305..730)
                     /gene="ssb"
                     /locus_tag="PLH17_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOR20334.1"
                     /translation="MINRVVLVGRLTRDPELRRTSNETAVASFTIAVDNRMKGPDGQK
                     TASFINCTVWGAAADNTAKYTRKGSLVGVEGRLNQRSYDRRDGTKASVLEVVCDSVQF
                     LEPKGEPRPEATGFTPDEQPAEDSKNLDAINIVDDDLPF"
     gene            complement(749..1036)
                     /gene="rpsF"
                     /locus_tag="PLH17_00715"
     CDS             complement(749..1036)
                     /gene="rpsF"
                     /locus_tag="PLH17_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOR20335.1"
                     /translation="MQKYEIMYILKADLEEAARQDEISKLHAILTNHGASVTDIKEWG
                     LKDFAYPINDLTKGYYVVIKVTADQPALLEFGRLARIDANVIRHLITVDAN"
     gene            complement(1261..2745)
                     /gene="lysS"
                     /locus_tag="PLH17_00720"
     CDS             complement(1261..2745)
                     /gene="lysS"
                     /locus_tag="PLH17_00720"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOR20336.1"
                     /translation="MIDETLTDQEQVRREKLDKYRELGVDPFGQKYEKSHDVSEILGL
                     TKGKTNEELETLNLRVKVAGRIMAIRRMGKASFVNIKDRSGSIQGYIGIDIVGDRAYE
                     VFRLADLGDIIGLSGRIMLTRTGEITIRVEEYVHLTKALRPLPEKFHGLTDVEERYRR
                     RYVDLIVNDESRRIALLRPRIIRAMQNYFDSQGFVEVETSVLSPILGGANARPFVTHH
                     NALDKDFYLRIATELPLKKLMVGGIERVYEIGRLFRNEGMDTRHNPEFTTVELYQAFG
                     DLQDMRHLAENVLRETALKVLGTTQVPWGDKIIDLGSPFRWVSMVELVKEKAGVDFNQ
                     EMTFEEAKAIAKKHDIHVEKHMTGIGHIINEFFDKYCEDDLIQPTFVHSYPIEISPLT
                     KMSPDPRFVERFELFVNGTEFANAYTELNDPLDQKERFLAQLKAKELGDEEANEMDTD
                     FLEALEYGMPPAGGIGMGIDRIIMLMANQTSIREVILFPCMRDK"
     gene            complement(2812..3777)
                     /locus_tag="PLH17_00725"
     CDS             complement(2812..3777)
                     /locus_tag="PLH17_00725"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOR20337.1"
                     /translation="MWKIANLEIESHVVLGPMAGVTSEAYRKFLKPFGVGLSFTEMVS
                     DCGLFYGNENTLAYLPTQNTDRPVGIQLFGSKIENMQKAIEIIQKANIPYDFIDLNFG
                     CPVPKVTKTGAGSSWLKKPQKIGEYVSQIVALSSKPVTAKIRLGWNDELVNFTQVVEQ
                     LEKAGVSAITIHARTTEQMYTGKADWNALAGLKMQMKIPLIISGDIFSLDDAIKALEV
                     TKADAVMVARGAMGKPRLVEQIDKYLRTGVRPPDATLFEQSEYLKKYAQLLIEEKGER
                     QAMMILRGIAPRFFNGYPGMKGLKSILSTNLVTKADLDTILSQIN"
     gene            complement(3802..5817)
                     /gene="ftsH"
                     /locus_tag="PLH17_00730"
     CDS             complement(3802..5817)
                     /gene="ftsH"
                     /locus_tag="PLH17_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOR20338.1"
                     /translation="MNEAKPRKSIFGYILPYLLMAVAIFAFVWLIISMQGKPEVWYLS
                     GNPAAGNDLDTNLNSYVIKTATSSSGYQTTKVSGIYHDPAKNKEGSYEVMIPNELFHN
                     SFAVVGETDDHPSYSSMFKAEIEEYNTLFPNADNGAFFVDTDAFEVSWWQNWGPTILL
                     LGGVVIVSLFFFSRLNNSVNSSNSKAMDFNRSRARREDSTKVRFSDVAGCDEEKAEMV
                     EIVQYLKEPRKFAKFGAKLPKGVLLVGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVE
                     MFVGVGAGRVRDMFRKAKQTAPCLVFIDELDAVGRQRGAGLGGGNDEREQTLNQLLVE
                     MDGFSDNSGIIVIAATNREDVLDPALLRAGRFDRQITVNLPDRRGREEIFKVHARGKT
                     IDETIDFGQLARRTVGFSGADIANILNEAAILAVRGGKDKVTIKEIDEAIDRRIAGPA
                     KSSRALNPHERRAIAFHEAGHAIIGLNLKHSDKVQKITIIPRGRTGGHVMMTPEEDRF
                     LLTKKELEARIIGYLGGRSSEEIFFDDISTGAHNDIEVATRIARLMVTEYGMSSLGPI
                     QYEKDSGSVFLGRDYTSTQKNFSTQIAFEIDTEVRHIIADAHARAKAIITEHKDDVIL
                     IAETLLEKETITAEEIEYLLKNRSLPTEEPKPGAPLTVAPAAEGESK"
     gene            complement(5989..7215)
                     /gene="tilS"
                     /locus_tag="PLH17_00735"
     CDS             complement(5989..7215)
                     /gene="tilS"
                     /locus_tag="PLH17_00735"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOR20339.1"
                     /translation="MIINYSFDTNATYLVACSYGPDSMALLHLLMQKTAKIVVCHVNY
                     HKRNISDFEEESLRAYCLEHNLGFEKFDAPKTATGNFQSWARELRYQFFADMYEKHRA
                     SALFVAHQQDDLIETYLIQKQRNSRVNYYGIEPVILMKNMTIVRPLLSFSKQDLLDYC
                     HENRVPFSIDVSNFETKYLRNKIRLQVVARLTEIERYQILKEINENNDMLLAQKSEVA
                     KKVQAKKELSIKSLLELSEKDFARAIDQFLKNRRKFIPISSAQIAEIKKACQSQTPNI
                     EIKLSNEISLFKEYDVLSLSGANQASYSFTLEKPGSLETDYFALDFSHGAEDRNVHDE
                     DYPLTIRTYKPGDKYEVGGHLCAVRRLFIDWKMPTRLRATWPLVVNKGGTIIYVPRYR
                     RDFAGDKKTKFEIKVV"
     gene            complement(7216..7485)
                     /locus_tag="PLH17_00740"
     CDS             complement(7216..7485)
                     /locus_tag="PLH17_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOR20340.1"
                     /translation="MRLDRYLKVSRLIKRRSVAKDVADGGRILINGKTAKPSSEVKVG
                     DEIEMHLGRNTIGVRVLMVNEHASLQEAEDMYEILYQENILERRS"
     gene            complement(7504..10875)
                     /gene="mfd"
                     /locus_tag="PLH17_00745"
     CDS             complement(7504..10875)
                     /gene="mfd"
                     /locus_tag="PLH17_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOR20341.1"
                     /translation="MLFAGLFHKKPANYAIITSNLYNAQSVYELLGALIGDDNVLLFP
                     NDDLLRAETISSSKELMAQRLYVMSELRKSKNKILIAHTSSLTRFMPSAELFESLSFN
                     LRVGMKVNLVELRQKLASGGYARVGKIDQSMQFASRGDILDIFSVNNPFPVRIEFFDD
                     EIESIRTFDIATQTSNERLSEISLTPASDVIFSEEEISRLEVNIEQQALKDATLLSND
                     EKEHLMSVTKEDFERIKSGQIHPRNYKYYGYAQREHYSILDYANFDIIFAVNEEAIMA
                     SLEISENEARAFLDELVDEGKIISHLAIYRPWNDIFKKLPKPLFSKKYVESIDDIEFK
                     VRPILSSSTSYMNILPTVQSYLSTNEKVVLALSNKQQFDTAKSLVKDADIVYETVEAL
                     ELPKGKLGMTLFDINEGFELVDERICYLSSKELLGYKTHRSRFMNRFKESTILKSFDE
                     LESGDYVVHEYNGIGQFIDIVTLEIDGNKRDFLHIAYAGTDVLYVPLSQFRLIRKYAG
                     KEGATPKLNRLGGNEWEKTKSRIKKRVNELAERLVVLYKERIRIPGYPFKKDDEIQKL
                     FEDEFPFELTADQSEALEEIKADMEKSESMDRLLCGDVGFGKTEVAFRAAFKAISNGK
                     QAAILCPTTLLARQHYERAIERFANFGVRIAVFSRLISEAQQRKYLKQVESGEVNLVI
                     GTHRLLSKDIVFKDLGLLIVDEEQRFGVEQKERIKEIKINVDVLTLSATPIPRTLQIS
                     LLGIRQLSQINTAPINRMPIQTYVMQYREDVIKELIERELSRDGQVFYVHNKVSSIYQ
                     VANRLQRKISAAKIGIVHGQMGREEIEDVMMNFYAGNLNILVATSIIENGIDIANANL
                     IIIEDADHFGLSQLYQIKGRVGRGSRIAYAYLFYREYKTINDKAVKRLKAIQDFTELG
                     SGYKIAQRDLMIRGAGDILGPEQAGFIDSVGIDLYLKMLSEAIEEKKTGVPIQEPRAA
                     HLFNVEAYIPANFAEKKDKIELYQEIESAKNLETLSKVSARMRDVYGRIPREVETLLR
                     QRRIDMLASGKAFSEILEYDDEINIVLSKEFSSINGIGNALFERIVPYLTKVKVSYIQ
                     RILKIKMPKNGDWMNDLEELLTIIGELYNVSVKKH"
     gene            complement(10986..11537)
                     /gene="pth"
                     /locus_tag="PLH17_00750"
     CDS             complement(10986..11537)
                     /gene="pth"
                     /locus_tag="PLH17_00750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOR20342.1"
                     /translation="MKLIVGLGNPGKKYEKTRHNLGYRVVDRLADSLGFDIDKEAFNG
                     FYAKESIFGEQVLLFKPTTYMNLSGTAVQEIASFFKIELPDILIIYDDMALEPGRIRL
                     RPAGSSGGHNGIQNIIEVFKTEDIKRMRIGIGEPENNGIDYVLGKPTKAQAELIDIAI
                     GEASEAIKDYLRNGFTSAMAKYN"
     gene            11647..12561
                     /locus_tag="PLH17_00755"
     CDS             11647..12561
                     /locus_tag="PLH17_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOR20343.1"
                     /translation="MKKYIKEKGIDLIKGLAIGVAFIIPGVSGGTMAVLLNLYDKIIN
                     SINNLTKRFVDSFKTLLPIALGAVLAILICWYPFKLAFEHIMIVMVTLFAGFILGGMP
                     GIFDEVKGIKIKPVHIVAMIISIVFAVALGALSVLFELNIQSLYDSRPWWLYILILLA
                     GIISSFALVMPGISGSMILIVLGFYTPTLNLIDNFLAWTDVGASISILATMAVGVVIG
                     VLLASKIIAFFLSRFRTGTFYAIIGIIIGSLIAIYYNYEIYDYYLTVGVRWWEILIAG
                     VALVAGIAVSYMIVRYSRNHKTIKEVSN"
     gene            12561..>13139
                     /locus_tag="PLH17_00760"
     CDS             12561..>13139
                     /locus_tag="PLH17_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOR20344.1"
                     /translation="MSLLSIVLSEAIEGLENVGRFWDLGTWEMTFLLWLNSLHGTFID
                     KVFLVITMLGDKGIVWITSGIVMLFFKKTRRIGIFVILTYGLIGGMNNFVIKKIAVRA
                     RPFYDTTIAVYPSAHVLQEYAVATFFGDGLFLGFGEIPDDYSFFSGHTVASIACATMM
                     ILHSRKIGIPAVILAVLIAFSRLWHGVHWPTDV"
     assembly_gap    13140..13239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13240..13426
                     /locus_tag="PLH17_00765"
     CDS             <13240..13426
                     /locus_tag="PLH17_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOR20345.1"
                     /translation="VLIAFSRLWHGVHWPTDVLFGTIFAVAITIGLFYLLRFLEPKVM
                     NLLTKIFKKNDRSAIEQ"
     gene            13555..14175
                     /locus_tag="PLH17_00770"
     CDS             13555..14175
                     /locus_tag="PLH17_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR20346.1"
                     /translation="MPKTPEQNQLIKDERKTRILHTALRLFALRGYDSVVINDITKEA
                     ECSHGLFYHYFKNKAEVFLELMKIAEERASKKREEEKDFNKVTAITAIRAIVSRMLDE
                     IYREDESPYFLYMFVNMHLQRTLPLPPKSKIPGPNKPFFHFFVDLIARGQKEGDVSGG
                     DPKEFAIIYYSIIKGLCYTRLSMINKSPTKVSPDIIMNLFTRKGNY"
     gene            14175..15968
                     /locus_tag="PLH17_00775"
     CDS             14175..15968
                     /locus_tag="PLH17_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20347.1"
                     /translation="MLKLFRFLKPKFWLIIIIFLMIGAQSFSQLMLPSLMGEIVANIT
                     GPTGADNSFIIRKGLEMIAYAGATIIIAFATSIGISYVGAYFGKKLRAEVFKKVLSFS
                     PGDYDRIGTASLMTRTTNDVEMVQTVVVMGLRIMVMSPIIMAIAIVQVLRTDVQLAII
                     LAFSIPLIIITIIILFAIAMPLFKKLQIAIDEVTKVLRASLTGVRVVRAFNQEEKEKE
                     RFAKVNRYQANLIIKVGRTMSFASPLISIFFDLTYFAIFLFGFAIIDGTANDARLDNI
                     LVTSQYAMHIMMSFLMFSMLLINIPRAGVSARRINEVLETKVDIVDPEVPHSENKGEV
                     GHVRFEDVTFTFPDSKIPTLQNITFDAKPGQVTAIIGSTGSGKSSIINLIPRFYDVSK
                     GRVLVDNVDVRDYTQKDLRDKIGFVPQQALLFSGTIRDNMHFGKPDGSDEEIRAALEV
                     AQAARFVARKENGYDAEVSQGGKNFSGGQKQRLAIARALVKKPEIYIFDDSFSALDFK
                     TDIRLRTALKEYTKTASVIIVAQRVSSIMDADNILVLNEGKIVGQGKHQELLKKCQVY
                     QEIVFSQMDESEIKRTLEIKKQVLLSEGGDE"
     gene            15968..17812
                     /locus_tag="PLH17_00780"
     CDS             15968..17812
                     /locus_tag="PLH17_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20348.1"
                     /translation="MPRSTDAMMGAKPKNLKKAASRILADFRPLLGVFIFVIIILLAS
                     AALSVVIPVLLGAFVNNFKNLATKVEGVYQVDWPEVFKQVIIMLSFYVGSALFIWLAD
                     WIVVKISADYAFDIRAKIKAKLDRLPLSYFDKNTYGEILSRGTNDVDNISRHMSSVIN
                     QTIIAVSMFLGTVIAMFITSWQLALVAIAILPFTILFTVLIAVNAQKEFKKYRSHLGK
                     LNAITEENFSGFKVVKLFNKEKDVAAAFEGVNEIMMKADWKSQWLSGFIFPTMRFIGN
                     LGFIGVSVVGGLLQDPGNMVAFFLFLQMFQQPFQQIGQISSIIQSVLASGERIYNLLD
                     EEEMESEEAGALSTEDAIKGEIVFDNVEFSYSPDKPLIQNFNLKVNIGDSIAIVGPTG
                     AGKTTIVNLIMRFYEVNSGRILLDGVNIRDYQRATLRGAIGMVLQDTWLFADSIRENI
                     RYGRSDATDAEIEEAAKQAHADHFIKTLPGGYNFVLNEDGTNISQGQRQLITIARAIV
                     SKPKILILDEATSSVDTRTELAIQDAMNKMMIGKTSFIIAHRLSTIKNAKVIIVMQKG
                     QIVEIGNHQQLLANNGFYAELYNAQFSGRNPLAEPEQESDNSEAKAAV"
     gene            17906..18850
                     /locus_tag="PLH17_00785"
     CDS             17906..18850
                     /locus_tag="PLH17_00785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR20349.1"
                     /translation="MAHSIAVSIIIPVRNSEKYLSSCLFSAVSQDFLLPYEVIIINNG
                     STDNSREIIDKYAKKYKFVRQIITDGLSVGFARKRGIEEAKGQYICFLDSDDMYKLSF
                     VKKMYETITANNVDIVNCSYVKINPNGRISRNVLARKRSLDCIHGVRSLLRDFNIRGY
                     MPMKMYRADLIKNTKLPISSKLILFEDFLINFALYSKASKTTTITEPLYFYRRTNESA
                     TTNLSSNRTDLHLKCFAAVRFLAEQTGDKAVIDEFFKRGFRYWLSIFADVFLSIKVNE
                     RPFFVELHRAEKIKRVILSRKPLPIKGMLWEQLIKEIS"
     gene            18973..21609
                     /gene="ppdK"
                     /locus_tag="PLH17_00790"
     CDS             18973..21609
                     /gene="ppdK"
                     /locus_tag="PLH17_00790"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOR20350.1"
                     /translation="MAKKYVYNFKEAHGLGKELLGGKGAGLAEMTHIGISIPQGFTVS
                     TEACTLYYNSGKILPDYLVKEIYDAIHGIEKETGKNFGGSHNPLLVSVRSGARASMPG
                     MMDTILNLGLNDNTVQAMANETSNERFAYDCYRRFIVMFANIAKGHPRTEMDKMLEEL
                     KEHRGYRLDTEITADELKELVKKYKAYYKKVFGEDFPSDPYQQAIEAITAVFRSWDNP
                     RANVYRKMNNIPYDWGTAVNVQSMVFGNKNENSGTGVAFSRSPSTGEKKVFAEYLPNA
                     QGEDVVAGLRTPLHIEELNTRMPEVYKEFVSTIGRMENHYHDMQDMEFTVEDGKLYFL
                     QTRSGKRTAQAALKIACDLVDEGIISEDEAVLRVDPNILDTLLHPSFDKKELAKTEPI
                     AVGLPASPGAGTGKLAFTAQEAKARHEAGEKVILVRIETSPEDIEGMVAAEGILTMRG
                     GMTSHAAVVARGMGKTCVAGASGIIVDEEKKTAKIGKKIYGVNDVFSLDGSTGNVYEG
                     AIKTVEAQLTGYFGRIMKWADERRTLHVRANAETPRDAQTAHNFGAEGIGLARTEHMF
                     FDKDRIFFMRKMILEETVEGREEALAELLPYQRDDFYKLFTIMGELNVNVRLLDPPLH
                     EFLPQEEDKIEELAKELDKTVDEIKARIDDLHEFNPMMGHRGCRLDVSYPEIGRMQTR
                     AIIEAALQAQKDTGKKITPEIMIPLTLDAKEFEFVKKIITETADEIIAASGQKLVYYV
                     GTMIEIPRAALLAGEIAKGSAFFSFGTNDLTQMTYGFSRDDAGKFLNDYYAKKIFEQD
                     PFRTVDQRGVGQLIKMSVENGRKANPDLHIGVCGEHGGDPVSVEFFHNAGLDYVSCSP
                     FRVPVARLAAAQAAIKAKAAKK"
     gene            complement(21678..23087)
                     /gene="glmU"
                     /locus_tag="PLH17_00795"
     CDS             complement(21678..23087)
                     /gene="glmU"
                     /locus_tag="PLH17_00795"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOR20351.1"
                     /translation="MKTYAIILAAGKGTRMKSLLDDKSKVSYPILGVPLVKHVLNALR
                     PLNIEKKVAIVGFGGPATEEIVRDEAETVYQHEQKGTGHAIMQAAPLLAGEQGRTLIV
                     CGDTPLLKTTTLQKLIEQHDQENNKLTVLSAIVPNPRGYGRIIRGSDGSVSEIVEQAD
                     VNEKQNAINEVNAGVYVFDNKLLFEYLDKLSPTNAQGEYYLTDLIKMFKNDGLKIGAS
                     TAGDYREMLGINDRRQLEEAAAIMRREINERHMLAGVTIEDSSSTYIGPDVVIQPDTV
                     IRPGTHIYGKTAIGRQNVIGPSTFLDNVVIGDENHIIFSHVADSNIGSANEVGPYARI
                     RGHSTIGNHTRVGNFVEFKACILHDGAKAAHLSYLGDVDIGEKTNIGCGTIVANYDGY
                     NKTKTVIGKDVFVGSGSTLISPIVVEDNAFIAAGSTINRSVPKDDMAIARARQENKPG
                     YGIVVRERALQKKLASQKK"
     gene            complement(23089..23943)
                     /gene="ispE"
                     /locus_tag="PLH17_00800"
     CDS             complement(23089..23943)
                     /gene="ispE"
                     /locus_tag="PLH17_00800"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOR20352.1"
                     /translation="MYIKAYAKINIALNVLGKDDNGYHQLDMVNLPLELHDSIEISVL
                     PDSSDSFITCDDIEIEETKYNLITIAINKAREKYKFKQQFTIHVYKDIPISAGLAGGS
                     ANAAAVLNALFQLLKIKPSRDETIELGKSIGADVPYCLFNIPSRVRGIGEIIEPIKIK
                     KGYHVLIVKPLKGLSTKNVFDRADGMELENGNIDNVIEALAKGDDDLLAKSVHNALEK
                     PSFSMLPEIAKIKDSLLKDGIKIVLMSGSGSSVIALEEDAKKLTKLEKKYAKMGYDTR
                     LTRTLRSK"
     gene            complement(23985..24800)
                     /gene="rsmA"
                     /locus_tag="PLH17_00805"
     CDS             complement(23985..24800)
                     /gene="rsmA"
                     /locus_tag="PLH17_00805"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOR20353.1"
                     /translation="MKFASQQAFFDFIKTLRFIASKELGQNFLVNEYIAENIVAKAKI
                     HKDDRVLEIGAGFGSLSYFISLGEGEITLLDVDPKIISFLTEQFDQKTNVKIVQESIL
                     KHDVSQYHKIIGNLPYYITSDTIQYCLLNARQAKTMIFMIQKEVLPRLIARFGEDGYG
                     PLALLLSFLGKTKRVFNVAKNNFVPAPKVDSVVIEIVLHENNDYETAEKLVKITNSLF
                     HHRRKTVRNNLRIYLDDIEKADKVLSELGVDGSKRPEELNLDFYLTLIKQLKF"
     gene            complement(24787..25329)
                     /gene="rnmV"
                     /locus_tag="PLH17_00810"
     CDS             complement(24787..25329)
                     /gene="rnmV"
                     /locus_tag="PLH17_00810"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HOR20354.1"
                     /translation="MKKKIDAIIVVEGITDIAFLNSFIDAEFVSTNGSDVPDTTIAYL
                     KEQSHIKPIVVLTDPDTPGQMIRTKLDANIPDLRHCFINKKDAVKNGKVGVAEAERKT
                     ILKALDFQLVTSHSMKSDLREIDLFKLGLVGTPNSSKIRIRISDRFHLGYVNGKQLLK
                     RARSLNLSYQQLEEAVREIR"
     gene            complement(25326..26099)
                     /locus_tag="PLH17_00815"
     CDS             complement(25326..26099)
                     /locus_tag="PLH17_00815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOR20355.1"
                     /translation="MIDSHCHLNHNSFYANPQKYIEEAMAKGVEAFLVIGYDLESSRR
                     AYALAQRFENVYAAVGIHPTDIKKRGPADLGQIEAMLSDKKVIGYGEIGLDYYWDKSE
                     EDQKEQKEYFIEQIKIANEHQKPIIVHTREAAYDTFKILSDNPPQYGGIMHCYSGSAE
                     MVGDYVDLGLYISLGGPVTFLNAKTPKEVAKITPLNRLLVETDSPYLSPHPLRGKTNS
                     PANLPLILEEIARLRGVEAKIIDEATTANFRRLFRLEKQ"
     gene            complement(26102..27421)
                     /gene="mnmE"
                     /locus_tag="PLH17_00820"
     CDS             complement(26102..27421)
                     /gene="mnmE"
                     /locus_tag="PLH17_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOR20356.1"
                     /translation="MHETIVALATPPLKSALAIIRLSGDDCFEVVSKVFSHDLRNTTK
                     RNVLVGHILKNDEIIDEVVLIAYVNPKSYTGENAVEIISHGSMLIVDEIIELLISSGA
                     RLALNGEFSSRAFLNGKIDLLQAEAIDDLINAKTHEAKKISLYSLKGETSNLVAPIKK
                     EIADLLSQIEVNIDFPEYEDIEEVSRAQVILVAERIIDNVTKLIAEGKQGQIIKEGIK
                     VAIIGKPNVGKSSLLNAMIGQEKAIVTNIPGTTRDIVEGEINLKGITLHILDTAGIRE
                     DSNEIEAIGIKKAKAASADADLVILVRDASQSDDDSFLALVKNKKHIIVYNKSDLAKD
                     KKQNTLYISALNKDIDPLLKEITDALSISQEAYDQPSLNNTRQLGLLGAIRESLYQAI
                     EDAKDNQPIDLVSVNLLSAYNATLEILGEGNKNDISEEIFSRFCVGK"
     gene            complement(27462..28058)
                     /gene="jag"
                     /locus_tag="PLH17_00825"
     CDS             complement(27462..28058)
                     /gene="jag"
                     /locus_tag="PLH17_00825"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOR20357.1"
                     /translation="MKKYQAKTIDEALEMAAQELGILPSDVNFAVQEEKKGLFKKVTI
                     EVYELSDAIEFAEEYLKNVTAALGIQSETTTVLKDDIIHISLNSDHNPILIGKNGKTL
                     QALNELARLAVSSKFKKRFRILLDINDYKDGKYSRVAFAARKAAKEVQKTKMDATLEP
                     MPADERRVVHNALANFSHIKTESAGEGHHRAVVIKYVD"
     gene            complement(28070..29518)
                     /gene="yidC"
                     /locus_tag="PLH17_00830"
     CDS             complement(28070..29518)
                     /gene="yidC"
                     /locus_tag="PLH17_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOR20358.1"
                     /translation="MKKSTKRILTFGGLFMAVVLISGCTASFCSNNDRANILYTYEKG
                     VSVYEDNAVGGVEVFAGNATLTRRVPKEEDKYQNSALLQTVIDEAGTNAINVPTEAYF
                     VAIDQEVLEVALTSQFGLSVDFTTVNAEQANLALTRFGYLKFAGYSEDNNLFETWDSW
                     TAELRVSLGAEQVPNQDFTKLYKEKLNTTISTYRACIAVNNGNYGKYGSDGNTEAFIE
                     GKSWAYAWSKGPIEGLLVYPVAFLVDYFSFTFGMNGWGQIGAVALVTLIVRSLILLTT
                     IKSTMGQQKMQALQPELAKIQAKYPNSNTNQAEKQRLAQEQMALYKKNKINPLGQILV
                     LIVQFPVFIAVWGAMTGAAVLSSDAVLGLNLSAGLGTSILNIAGWPNAAGWWTAVVLF
                     VLMGISQFISMKLPQWMQKARTKNVKKLSKNPAQDKQSKQMKWFSNIMLVMIVVMGFS
                     LPAAMGVYWFIGAIISVIQTLIMQTIMARSKK"
     gene            complement(29502..29849)
                     /gene="rnpA"
                     /locus_tag="PLH17_00835"
     CDS             complement(29502..29849)
                     /gene="rnpA"
                     /locus_tag="PLH17_00835"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOR20359.1"
                     /translation="MKRQYRLRKSEDFHRIIHASRFVKNQLFVVYFQPNNLNAVRVGI
                     SIPKKIGIAVTRNKIKRQIKAILRETLDVSTSIDYVIVPKHNYNVEEFNKSHDALKGL
                     LEKIGDHNFEKIN"
     gene            complement(29884..30018)
                     /gene="rpmH"
                     /locus_tag="PLH17_00840"
     CDS             complement(29884..30018)
                     /gene="rpmH"
                     /locus_tag="PLH17_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOR20360.1"
                     /translation="MKRTYQPSKKKHSAVHGFRERMKTPNGRKTLARRREKGRAVLSA
                     "
     gene            30497..31852
                     /gene="dnaA"
                     /locus_tag="PLH17_00845"
     CDS             30497..31852
                     /gene="dnaA"
                     /locus_tag="PLH17_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOR20361.1"
                     /translation="MTSSISEISKLWDRILDSTKSRINDPRIFDTFLNSSYINSIDGD
                     TMVVVVNSGLAANILSTKYLDMITGVVRECTETDFKLKFVQASEVQTVAKQAATKPTF
                     FANSYINSKLTFSNFVVGSSNLEAYQASIAIASSLGKLFNPLFIYSQSGLGKTHLLHA
                     IGNYIKENAPVSKVLYLSTEDFFEEFVKYVTGDKEGENLKDFFKGIDVLLIDDIQFLA
                     NKIKTEEMFFYIFSSMINAGKQIVLTSDKHPSELQGVEARLVSRFNAGLTVNIATPDV
                     KTCVDILKLKISANNLDIANFDEDVLTFFAEKFSKNVRELEGAFNRLLFYTINIKPTA
                     HIDMGVAIESVQSLIDVKDTKTKLSETKIINVVADYYNLTPSQLTGKIRTSQIAMARH
                     IAMYLIRSMLDVSLTKVGMVFGGKDHTTVMNAVEKVDKVLKTDTFLQTAIDDLKKRLK
                     K"
     gene            31989..33110
                     /gene="dnaN"
                     /locus_tag="PLH17_00850"
     CDS             31989..33110
                     /gene="dnaN"
                     /locus_tag="PLH17_00850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOR20362.1"
                     /translation="MRLTITREHLLKGLTTAAKAIGSKVPVPLLMNLKLVLNEKGLTV
                     VGGNNELTISTLVPNMIGEKEIIRNAKYGATLVASKIITEIARRMEGEELAIEVVDET
                     IVKIDDGKSSFKLNSIRADEYPEVDLDPSGVVFDISCHDFTRLIEQTAFAASNKEQRP
                     ILTAVNLEAAGGKLIATATDSARLARKEVPIDDVVSFVSNVPARTLIEITKMFEGAEK
                     VTLAVSDKKILFSFDNTLVSSRLINGDYPNTKNIIPKTFNYFLEVNSAELISAMERVS
                     LLSVERENVVKITMTEDALEVASKSAQVGSANEKLSTFQFSGDRLEMSFNSDYVVSAI
                     RAVKAEDVTIAFIGEMKPFVIKNNKDDSVVQLVTPVRTY"
     gene            33219..33443
                     /gene="yaaA"
                     /locus_tag="PLH17_00855"
     CDS             33219..33443
                     /gene="yaaA"
                     /locus_tag="PLH17_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HOR20363.1"
                     /translation="MIKKTVEITISTDFVTLGQLLKIADLIGSGGEAKSFLASHEILV
                     DGVSDNRRGRKLRDEMVVEIDQVGYRIVKK"
     gene            33440..34513
                     /gene="recF"
                     /locus_tag="PLH17_00860"
     CDS             33440..34513
                     /gene="recF"
                     /locus_tag="PLH17_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOR20364.1"
                     /translation="MIIRNLVLRNFRSYDFAELSFDKGLNVIVGENAEGKTNIVEAIH
                     YLSLARSFRTAEDANLIREKSDFAAIEAEVSEGSTKKKINVVLTSSGKKVSCNGKAIN
                     RLSELSELVNVVVFEPPRVLLFKESPKARRDFLDISLSKQSSIYLEYVTKYERALRER
                     NELLKLDKPNEVQLEIVTDLLIEVAEKVVRYREMYVEQINQVLSKIVTTIKGEQENAR
                     IRYQPFARPDASFKKNAKSAFSKALESDLRKKVTSIGPHREDFKMSLNGKDVARYGSQ
                     GENRILALALTLSPYFLIEDKNKRPIVVLDDVMSELDDENKERLISFLTKFEQVFITS
                     TSLEVKNASVYEVKNHKIARRNS"
     gene            34513..36507
                     /gene="gyrB"
                     /locus_tag="PLH17_00865"
     CDS             34513..36507
                     /gene="gyrB"
                     /locus_tag="PLH17_00865"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOR20365.1"
                     /translation="MEEKEKVTTFPVDDEDEKGSADYGARDIQVLEGLAAVRKRPGMY
                     IGTTSSAGLHHLVWEIVDNSIDEALAGYCDRVALTINKGDTITVCDNGRGIPVDIVEK
                     TGLSGVETVFTILHAGGKFGEGGGYKVSGGLHGVGGSVVNALSEWMDINVHKGGKNYF
                     LRFERGGKPVGHLQVVGDASDRGTIVTFKPDPTIFIESVEFNYEIIRDRMRQMAYLNR
                     GVRMIVTDDRGETPVTEEFYYEGGIKEYVTHINRNKEPLFEDVIYCEGKDNYAFPDGK
                     IAHIHAEIAMQYNTSYNPTIFTFCNNINTHEGGMHEAGFTLAINRVVNDYAKANGYLK
                     DVEENLATDDIKEGLTAIVSVKHPNPQYEGQTKTKLGNSEVRKIVSGIVGDGLNRFLL
                     ENPKLAKIMMDKIVNAAMARIASRKAREQVRRKTVLDITTLPGKLADCSSRNPEECEL
                     YIVEGNSAGGSAKNGRDRVTQAILPLRGKILNVEKAQPHRVFENAEIGNMITAIGGGY
                     SAEFDVSKIRYHKIVIMTDADVDGSHIRILLLTFFYRFMRPLIEHGFVYIAQPPLFKV
                     EFRGKQYYCYNDEQLEVLKKKLDLKPGYPFQRYKGLGEMDAQQLWETTMDPRARKMMR
                     VSLTDAIEAEAVFTDLMGDAVLPRKEFIHKNAKFVKNLDI"
     gene            36526..39093
                     /gene="gyrA"
                     /locus_tag="PLH17_00870"
     CDS             36526..39093
                     /gene="gyrA"
                     /locus_tag="PLH17_00870"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOR20366.1"
                     /translation="MEEKEKIVTPDEEKEIEEIESGIVPGLRDVDISKEVRSSFLDYS
                     MSVIVSRAIPDVRDGLKPVHRRIIYGLNESGMQPDKPYKKCARIVGDVMGKYHPHGDA
                     AIYSTLVRLAQPFSMRYTLVDGHGNFGSVDGDEAAAMRYTEARMTKLAMEMVRDINDD
                     TVPFVDNYDGTEKEPSVLPSRFPNLLVNGSAGIAVGMATNIPPHNLNETIDATIAVAR
                     NPLLTPLEIMEKYLPGPDFPTGGTILGRGGIKDAYETGTGSIALRSKALIEEGENGKK
                     RIIIYEIPYQVNKARMIESIANLVKEKIIDGITDVRDESNKEGIRVVIEVRRDTIPEV
                     ILNQLYKNTQLQVSYGIIMLCLVEGAPKVLPINEILLGYIDHQVVVIEKRTKFRLAKD
                     EARDHVVLGLLTAIDNIDEIVEMIKKAATPEEASQKMMIRFDLSEIQTQAILAMTLRR
                     LTGLEKQKLEAERAQLEINIAKYRDILSSREKVVDVIVEELLEIKGRFGDERKTEISN
                     ELATIEDEKLIPEEDIVIALTVNNYVKRMTTDTFRTQNRGGVGVKGINTNENDIVSIL
                     VQTKTHTDVLFFSNLGKVYRMRGYQVPESGRVSKGTPIQNLIFNLEKEERIMAIISLD
                     DYHEEHFLFFITVNGIVKRTALSEFASIRQNGKIAISLKEGDALLDVKRTDGNALIGL
                     ASSNGKMVKFNESDVRSMGRTAAGVKGMSTDGAEVVSATTSIEGELVLAITQKGYGKM
                     SPAESYRLTKRGAKGVLTINTTDKNGKLVALRAVNGDEDLMIITANGTIIRIPLEQVK
                     VAGRNTQGVRVIKLDGRQKVVSIAIVPHEENGVAEEKSNGSEEEENVAIPEENDADI"
     gene            39099..40118
                     /locus_tag="PLH17_00875"
     CDS             39099..40118
                     /locus_tag="PLH17_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20367.1"
                     /translation="MKVFVHFQPRYLKDVYEGMRLRKNIKGALELTNIEIAKNSIDYY
                     DVAHFISVADEMKINDAIELGKPVVFSALMCESDPAAKITEITRDGEVRLSNRALRIL
                     NKVSAVIVPNEAGVKFLSEQGVKTKIIVLSPGVNIARFEKSDQLEADLFYRYFRLSKS
                     AKTVVAFGQYDSREEIRNIIEIAKLCPEYKFLYFGQAKRLFFLPSKIRRLLRRSPKNL
                     RFNPIIPEDVYRSVMMNANVFLLINRRKYNAITMLDAIAAKCQIIGLGPIDYDAKVLA
                     KGYAYIANTLPAVAASINQFFIGELPSKAVKAYNIAKAESLTEIGKQLEKIYTGLIAI
                     DKGGR"
     gene            40120..41400
                     /gene="serS"
                     /locus_tag="PLH17_00880"
     CDS             40120..41400
                     /gene="serS"
                     /locus_tag="PLH17_00880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOR20368.1"
                     /translation="MLDIKLITENPQKIQEGLKKKLFEVDFEPLLSLVEERKKLLIKV
                     ETSKAEQNRLSGSVPHIKKSGGDVSAIFAKVKELSAQNAADEAHLKVVEEQIFDFVAG
                     LPNIPDEDLLPGGKENNRVVYEYGKMPTFDFKIKDHVELAESLGLVDYERAAKISGSG
                     TWIYTGQGAMLEWGLINYFIANHLQDGYQFILPPHILNYESGFVAGQFPKFNDAVFTL
                     EGVTPKKFILPTAETALVNLHRNEILDANSLPRKYFAYTPCYRSEAGSYRTEERGMIR
                     GYQFNKVEIVQYTTEEGSDAAFEEMVGKARRLVEQLGLHFHVSKLAAGDCSHAMARTY
                     DIEVYIPSMATYKEVSSVSNARDYQARRGMMRYRDKVTGKTKFLHTLNASGLATSRLV
                     PAILEQFQQADGSVIVPEVLRPFMGGISKLEAKK"
     gene            41426..41522
                     /gene="ffs"
                     /locus_tag="PLH17_00885"
     ncRNA           41426..41522
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLH17_00885"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            41519..41986
                     /gene="tadA"
                     /locus_tag="PLH17_00890"
     CDS             41519..41986
                     /gene="tadA"
                     /locus_tag="PLH17_00890"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOR20369.1"
                     /translation="MKKNDEYYMLLALKEAMKAQAIDEVPIGAIIVKNDKVISRGHNQ
                     REKRNDPTAHAEIIAIRKACKKLGVWRLDGCQLYVTIEPCSMCAGTLLWTRIDRIVFG
                     AKDPKGGALGSSYHLFEQKNLNHHPVICGGVLENECGQLVSAFFQNKRLLQSK"
     gene            42063..43076
                     /locus_tag="PLH17_00895"
     CDS             42063..43076
                     /locus_tag="PLH17_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20370.1"
                     /translation="MEKKTLNFILVTLAFFLAGCAGTPDSTSDSESGGNFTFCEDDSA
                     FFDFSEDDTLNAAIEELPLGLQTDEVRDDILEILGSNDGYANHVSYSLEIMTDYDVLS
                     TLDDDRDFFGEYAKEVSRYDAEKVLSGTYDVDENYYDEAESEEVTVDLTADYQVFRNG
                     FCPSISRYYEIYNFAGEDNDVALEKVYGTENFIRKLYLIDGQDEALKLNNHNDDYEED
                     ENVEDLTFTATKFLSKADGEIDADPGLNTSTSLEISVIASYDPAGAALGGKEIHSILI
                     VDGMIKKVRDFSGTFEVVGIDEIYRTSEEKIATFSVQDNGAFTGTLVNPDDFTFSNTA
                     PKL"
     gene            43296..44225
                     /locus_tag="PLH17_00900"
     CDS             43296..44225
                     /locus_tag="PLH17_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOR20371.1"
                     /translation="MFIRPDVERAIKLNRPVVALESTIISHGMPYPENVKTALMLENI
                     ISEAGAVPATIGIIDGDIVVGMSPEQIEEFGKRRDIRKVSRRDLPVVVAKKEWGATTV
                     AATMIIAEKAGIEVFVTGGIGGVHRNAATTFDISADLEEFAMTDVTVISSGAKAILDL
                     PLTLEYLETKGVTVLSYQSDEFASFYSRDSGLKIEDRIESADEAAAIIKNKRDLKLRG
                     GILISNPVPREAEMPKAEIDQAIDEALGKAEKNGIKGKEVTPFLLREIATITKGKSLA
                     ANIRLIKNNALLGAAVAKALAQLNTKGNKHEKT"
     gene            44212..44808
                     /locus_tag="PLH17_00905"
     CDS             44212..44808
                     /locus_tag="PLH17_00905"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR20372.1"
                     /translation="MKKLNSFRGALFSLAQEKPLAKISIEELCDRVGVKRQTFYYHFK
                     DMGDFIDAILVDICEKRSDKEGDLNQDARFIADLYANNSEILKNFLTSPYAINIINFV
                     HNYLHVRSYNRLAANAKYKSLPGEQIKSVSRIVASIIVSELSYWINTGMEEDKNHLLA
                     RFDAILKNVEKMMVENALRGTIEKRKHPELAEDEKNKH"
     gene            44823..45995
                     /locus_tag="PLH17_00910"
     CDS             44823..45995
                     /locus_tag="PLH17_00910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR20373.1"
                     /translation="MIDFDFVAPTKLFFGRGKEKDVGAIIKSYGFKKVLFHYGTGSIK
                     SSGLYQTIVSSLKNNDVDFIEFGGVRVNPTHSMVRAGVYLARKEDIDFILAVGGGSVI
                     DSAKAIAAGFFYDGDPFDFNLHLAKPKQALSVGVVLTIAAAGSELSASCVISNDETGL
                     KRGFNSDLNRPLFAICNPELTYSVSKYQTGCGIVDIISHSFERYFSPSEKKELADEFA
                     LGVIRHVVEVGKAAIDNPGDYDARASLMLASSFSHNGITGLGKKVNMPIHAIAHGLSG
                     HDPRIAHGAALSVMIPAWMAFVKDAEIDKFSAFAREVFKIHFVSREESANIGVRELRR
                     FFEEIGMPVTLRELGIDKQDIPRLVAMITENGTRTVGHSVRPLDAKDIEALLLSCL"
     gene            46011..47114
                     /locus_tag="PLH17_00915"
     CDS             46011..47114
                     /locus_tag="PLH17_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20374.1"
                     /translation="MKKEDAASLIVYAVMIGLALFVGLGIIQPVFAANASVGGSQYAF
                     AIISVIIGVFINAILLEVGHILGGLIGKYRIHSVNIFGFCFHKKSGKTHFAFRMFDGL
                     TGETKFLPKSEKSNPRPATLLPVLLYLIELVAAIILYALISTNVDVDLVWLSIGAIIV
                     ASIGGMMTLYNIVPFKLDAITDGYRLTLFAKKINIVAYNELMLAENGDEPFTPRIFDE
                     ITDFTAEVNLISVYRNIKEKKFAEAETILTNIIANEAKTSNSTHNRAIAQLLFLKIMN
                     EPLEDAKAYYATIPTSIHRFIANDLSMPSLRAYVLIAGLMDDAQGEIMYCLDRKKKAI
                     KYTAPGIAEIENSLFKEALDKVKVAHPDWKLEQ"
     gene            complement(47165..47665)
                     /gene="mscL"
                     /locus_tag="PLH17_00920"
     CDS             complement(47165..47665)
                     /gene="mscL"
                     /locus_tag="PLH17_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008794452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOR20375.1"
                     /translation="MKIFKEFKDFISRGNVMQLAVGVIIGGAFSAIITALVAHVFMPL
                     IGLLVNGGLNNFYTVLPNSIKATTAEILAGAAVLANNGIYYSVLSKLDWGLVLTAIIN
                     FLLIALILFAVIKIFASIQKAKEAALAKLHKKEGEPEPEPAPTPAPAADIVLLTEIRD
                     LLKKDK"
     gene            47795..48646
                     /locus_tag="PLH17_00925"
     CDS             47795..48646
                     /locus_tag="PLH17_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOR20376.1"
                     /translation="MNKVAIFVDSTADLGKEQYNKLDIHVVPLLVNIGGNAYKDGEEI
                     DSTKLYQLVAEHGELPTTAAVPLYTFVEAFKPFIDKGYDIFYVGIGGKLSATLQSVVL
                     AAQEFPEGRVFYSDSNNLSSGSGILALKAVKYRDEGKSAADIKALIDAHAPKVNSLFA
                     IETLDYLHKGGRCSGTAKVLGKLFHIHPIIKVTDGKLIVYKKPRGRMSSALDELAGEL
                     KSHLPHVDSDHIMITHAGVEQANLDYLVKKVGALVNPNSVMITNAGAVISSHCGYGTI
                     GVLYLKK"
     gene            complement(48710..49450)
                     /locus_tag="PLH17_00930"
     CDS             complement(48710..49450)
                     /locus_tag="PLH17_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOR20377.1"
                     /translation="MRHSLSFLILSTLILSSCSSFGDSSVSPSDVDTSSSDTSISSEH
                     ISSDTATDTSTGASPSESTGEVPVGEYRLNPVPGTGNIGFVYDEYGQVLKEIYKDEYY
                     TDVEDVAAYIVAFNDVPDNYYFTTERNGYGESKDVCYGLYGNECRIYPGPYESNYSFL
                     PYSLDDVYNEADIGGDGYAESSSWNRGALRVVFTLGGIYEYGNNIPVVFYTDNHYDTF
                     YEYKNYAGGWGPEFGKNGYDWSPIETWF"
     gene            complement(49467..>49816)
                     /locus_tag="PLH17_00935"
     CDS             complement(49467..>49816)
                     /locus_tag="PLH17_00935"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOR20378.1"
                     /translation="RGGSKIGVGFVNPSIKPGRKSEADLLAKIKTGVYISSVAGLHAG
                     LNPQSGNFSLQAEGFMIRDGKLAEPLSLITVAGNLLDLFMNVREVANNAELQLSGISA
                     PSLLIKNLAISGK"
     assembly_gap    49817..49916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<49917..50779)
                     /locus_tag="PLH17_00940"
     CDS             complement(<49917..50779)
                     /locus_tag="PLH17_00940"
                     /inference="COORDINATES: protein motif:HMM:NF040244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOR20379.1"
                     /translation="MNYKKFFELAKSKGIEASELYSAKKYSLSFELFHSEVSSYSTSE
                     TFSMSARGIYNDKFGVVRTEKVDAKTPEHLVDEIISNAKVIEKADKALLFKGSDKYQK
                     RNLYNKDIPAISIDQKMKNLFEIEAKLKKADPRIVEIEGVQYSEDAIEVNLVNSYGLN
                     LKSKSNYYVYAASVVVKQGEEVKTGYKVLLSNDPKELDIDKFVDQVVENATSKLGGTQ
                     CDSKKYPVILNPKTAASFLDPYLESASAEEVQKHSSLFIGKLNQQVASKKVTILEQPL
                     LKNCFFRYFDD"
     gene            complement(50776..52173)
                     /locus_tag="PLH17_00945"
     CDS             complement(50776..52173)
                     /locus_tag="PLH17_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOR20380.1"
                     /translation="MIGKSLAIDVLNAGLVTGADFAEIYLEQNDSSFLSLENGKTESS
                     LSSTTYGAGIRLLNKLQSVYGYTNDISRKGLMNLATSLAKSFQGERLITVEKINKVRV
                     KNAHVYERPLSEVSREEKIALMKEAHEVISSYDKRIVRIQCSFMNNRKVVTIFNSDGK
                     QFADFKERGRMAFVAIASENGKIETSFEGPGATAGMEFFTAKISVRDTAKRVAELAIK
                     MLTAAECPSGKMPVVIGNGFGGVVFHEACGHSLEATAVAKKLSVFSDSLGKQIASPLV
                     TAIDDGTIANGWGSNNIDDEGNPTQRNVLIKDGICTSFLVDSFNGRRMNAKANGACRR
                     ESYKYEPTSRMSNTFIAAGNSTHEEIIAATKLGLYAASMGGGSVNPATGEFNFSVSEA
                     YIIRDGKIAEPVRGATLIGSGADILMKIDMVGNNLQRAQGMCGSVSGSIPADVGQPTI
                     RVSEILVGGRGGVLK"
     gene            52250..52858
                     /locus_tag="PLH17_00950"
     CDS             52250..52858
                     /locus_tag="PLH17_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20381.1"
                     /translation="MYNNFMFDAELLENLEKHFQEKKVLFKKEDFAYIAGKDTFKGKM
                     LGFLTTDYYRREKLIRGGSLVYGYVFRTWTNEVTFTRPYPMWILFSPSQAFKNDPDLF
                     VSILSALQAIELPKRGQSELRKLFTMLNAELSEPKYFLIPEPYAQGHLVYLSMAYHRP
                     CHNNNLKLGINPFIMGASISKEILYLPTKYWDESFKKAYYEF"
     gene            52962..53171
                     /gene="rpmE"
                     /locus_tag="PLH17_00955"
     CDS             52962..53171
                     /gene="rpmE"
                     /locus_tag="PLH17_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOR20382.1"
                     /translation="MKKDIHPKYNRVTVTCVTCGSVFETGSTMKEIRVDTCSNCHSYY
                     TGKQRFTQADGRVDTFNKKYGLKNK"
     gene            53207..>54477
                     /gene="metG"
                     /locus_tag="PLH17_00960"
     CDS             53207..>54477
                     /gene="metG"
                     /locus_tag="PLH17_00960"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOR20383.1"
                     /translation="MMKTKFYITTPIYYPSAKAHIGHAYTTVVADVLARYKRMRGFAT
                     YYLTGTDEHGEKIQNNAKLAGKSEQQFVDDIANDFKKLWKTLEISNDDFIRTTEERHT
                     KVVKSIFTKLYNQGDIYLGKYEGWYCTPCESFWTETQVGPERLCPDCHRPVHTASEDA
                     YFFKTRKYVDQLLKIMDDNPKFLLPVNRKNEMINTFIKPGLEDLCVSRTSFSWGIPTN
                     EDPRHVVYVWLDALSNYITALGYDSDNPELFNKFWADENSEIVHLMGADISRFHAIYW
                     PMFLMGLGVRVPDRLFVHGLLMMKDGKMSKSKGNVVSPYPLIERYGVDAVRYYLTREV
                     VFGSDGQFTPEQFVERINNDLANDFGNLLNRTLAMINKYFEGVVPTYDGDVTPFDKDL
                     RTLAEETVKGYEEDLDDLKITDAFSKVNNLVA"
BASE COUNT        14345 a        12762 c        12477 g        14693 t
ORIGIN      
        1 ggtgagaacg gattccgccg tcccatttcg gaagaacgac ttcgtcctgt cacatctctt
       61 cagcttagtc gattacgtaa gggagaaggg ccatgaaacg tgcgtttttg atggctttgg
      121 cgagctctct ttgatatctg gcggccgttc cggtagagcg gcgcggcgag attttgccgt
      181 tcggcgtaat aaatctggag agcagttcga catctttgta atcgatgtgc gtgattttat
      241 tttttgtaaa atagcaaacc tttttgcgag gtcttacttt tttgtaaccc attgtttgtc
      301 ctttctagaa tggtaggtcg tcatcaacaa tattgatggc gtctaagttt ttgctgtctt
      361 cggccggctg ttcatcgggt gtaaatcctg ttgcttcggg gcgaggttcg cctttcggtt
      421 caaggaattg aacggaatcg cagacaactt cgagaacaga agctttggtg ccgtcgcggc
      481 gatcatagct ccgttgattg agacggcctt cgacaccgac cagtgaaccc ttgcgggtgt
      541 atttggcggt attgtcggcg gcggcgcccc acaccgtgca attgatgaag ctggcagtct
      601 tttgaccatc aggtcctttc atgcgattgt cgacggcaat tgtgaaggaa gcaacggctg
      661 tttcgttgga tgtccttctg agttcgggat cgcgtgtcag gcggccgact aaaacgactc
      721 gattaatcat tatttaatcc tccttgcctt agtttgcgtc aactgtaatc agatgacgaa
      781 ttacgttggc atcgattctg gctaagcgac cgaattcgag caaggccggt tgatccgctg
      841 tcactttgat gacaacgtaa tagcctttcg ttaaatcgtt gatcggatag gcaaaatctt
      901 ttaatcccca ctcctttatg tcagtgactg acgcgccatg attagttaaa atggcgtgga
      961 gtttggagat ttcatcttgg cgggcggcct cttcgaggtc ggctttcaag atgtacatga
     1021 tttcgtactt ttgcattagt acacctccct tttggtcatt cggctactat ctgctagtag
     1081 cggagagaac gcgataaaaa tatcgcaaaa caatcatagt atatactata actaaagtaa
     1141 agtattaatg cgcccggacg ccagtgcgac cgagctcaaa cgcaagcgat tgggtcagat
     1201 cgcttttccg tctgtctctc ctcccttgac tgatatataa gggcgagcga tgaaaaatgt
     1261 ttatttatcc ctcatgcaag ggaaaagaat tacttcgcgg atggaggttt gattggccat
     1321 cagcatgatg atgcgatcga tgcccatgcc gataccgccg gcgggaggca ttccgtattc
     1381 gagggcttcg agaaaatcgg tgtccatctc attagcttct tcgtcgccga gctctttggc
     1441 tttcagctgg gccaggaagc gctctttctg atcgagggga tcattgagtt cggtgtaggc
     1501 gttagcaaat tcggtaccgt taacaaataa ttcaaaacgt tcgacgaaac gcggatcagg
     1561 actcattttt gtgaggggcg aaatctcgat cggataggaa tggacaaacg tcggctgaat
     1621 caaatcatct tcgcaatact tatcaaagaa ttcgttgata atatggccga tgccggtcat
     1681 gtgtttttca acatgtatat cgtgtttttt agcgatggct ttcgcttctt caaatgtcat
     1741 ctcttgatta aaatcaacac ccgctttttc cttgacgagt tcgaccatgg aaacccacct
     1801 aaaaggtgag ccgagatcaa ttatcttatc gccccacggc acttgcgttg tgcccaatac
     1861 tttaagagct gtttcgcgca ggacgttttc ggctagatgc ctcatgtctt gaagatcgcc
     1921 aaaagcctga tagagctcga cggtcgtgaa ttctgggtta tggcgggtat ccatgccctc
     1981 gttgcggaaa agacggccga tttcatagac gcgttcgatg ccgccgacca tcagtttttt
     2041 aagtggtaac tcagtggcaa tgcgcaggta aaaatccttg tctagcgcgt tgtggtgagt
     2101 gacgaaaggc cgggcgttgg cgccaccgag aatcggtgag agaacgctcg tttccacttc
     2161 gacaaaacct tggctgtcga aatagttttg catcgccctg ataatgcgcg gtctgagcaa
     2221 agcgatgcgg cgtgattcgt cgttgacgat taagtcgaca taacgacggc gatagcgttc
     2281 ttcgacatcg gtcaggccgt ggaacttttc aggaaggggc cgaagggctt tcgtcaaatg
     2341 gacatactct tcaacgcgaa tggtaatttc gccggtgcgg gtaagcatga tgcgaccgga
     2401 gagtccgatg atgtcgccca gatcggcgag gcgaaaaacc tcataagctc tatcgccgac
     2461 gatatcgatg ccgatataac cctgaatcga gccgctgcga tctttaatgt taacaaagga
     2521 ggctttaccc attctacgaa tggccataat gcgtcctgcg actttaacgc ggagatttaa
     2581 agtttccagt tcctcgttgg tcttgccttt ggtaagcccg agaatctctg aaacgtcatg
     2641 acttttttca tatttttggc caaatggatc aacgcctaat tcgcggtatt tatcgagctt
     2701 ttcgcggcgt acttgttcct ggtcggttag tgtctcgtct atcatagtat ttcctcttgt
     2761 tgcttatata agcatatcat ttactctttc aatttcaaaa cgaacaatgc attagttaat
     2821 ttgcgacaaa atggtgtcca agtcggcctt cgttacaaga tttgtggaaa ggatactttt
     2881 tagccccttc atgccgggat agccattaaa gaagcgcggg gcgattcccc ttaaaatcat
     2941 catcgcctgc ctctctcctt tttcctcaat gagaagctgg gcatattttt ttaaatactc
     3001 gctttgctca aaaagcgtcg catcgggagg gcgaacccca gttcgcaaat atttgtcaat
     3061 ttgctcgaca agacgaggtt ttcccatcgc tccgcgagcg accatcaccg catcggcttt
     3121 tgtgacttca agggctttga tagcatcatc aagactgaaa atgtcaccgc ttattatcaa
     3181 cggaattttc atctgcattt taagtccggc aagggcattc cagtcggctt ttccggtata
     3241 catctgctcg gttgttcggg catggatggt aatggcggaa actccggctt tctccagctg
     3301 ttccaccact tgagtaaaat taacgagctc atcattccag cccaagcgga ttttggcggt
     3361 caccggcttt gatgaaaggg cgacaatttg agacacgtat tcgccaattt tttgcggctt
     3421 ctttagccat gaagaacccg cgcctgtctt cgtgactttc ggaaccggac agccgaaatt
     3481 aagatcgata aaatcgtagg gaatattggc tttttgaata atttcgatgg ctttttgcat
     3541 gttttcaatc ttgcttccga agagttgaat tccgacgggc ctatcggtgt tctgggtagg
     3601 caaatatgca agtgtatttt cgtttccgta gaagagtccg cagtcgctta ccatctccgt
     3661 aaaactgagg ccgacgccaa aaggtttaag aaacttgcgg taggcttcgg aagttactcc
     3721 ggccatcggg ccaagcacga catgcgattc aatttccaga ttggcaatct tccacatacg
     3781 aaaaaaggaa ggttgccctt cctatttgct ttctccttcg gcggccggcg ccacagtgag
     3841 cggcgctccc ggttttggct cttccgtcgg aagcgagcga ttctttagaa gatattcgat
     3901 ttcttcggcc gtaatcgtct ctttttcaag gagcgtttca gcgattaaaa tgacatcgtc
     3961 tttatgctcg gtaatgattg ctttggctct cgcgtgggca tcggcgatga tgtggcgcac
     4021 ttcagtgtcg atttcaaagg cgatctgcgt cgagaagttt ttctgagtcg acgtatagtc
     4081 gcggcccagg aagacggaac cggaatcctt ttcatattga atcggcccaa gcgaactcat
     4141 tccgtattcg gtcaccatca atcgggcgat gcgcgtcgcg acttcgatgt cgttgtgggc
     4201 accggtcgag atgtcatcga agaatatttc ttcagaggag cgaccgccga gatagccgat
     4261 gatgcgtgct tcgagttctt ttttagttaa taagaaacgg tcctcttcgg gggtcatcat
     4321 gacgtgcccg ccagttcggc cgcgaggaat aatcgttatt ttttggactt tgtccgaatg
     4381 ttttaaattg agaccgataa tcgcgtgacc ggcttcgtga aaggcgattg cgcgccgttc
     4441 atgagggttg agagcgcgtg agctcttggc ggggccggcg atgcggcgat cgatggcttc
     4501 atcgatttct ttaatggtta ctttatcttt gccgccacgg acggcaagaa ttgccgcttc
     4561 gtttaaaatg ttggcgatat cggcgcctga aaaaccgacg gtgcggcggg cgagttgacc
     4621 aaaatcaatc gtctcgtcga tcgtcttgcc acgggcatgg actttgaaga tttcttcgcg
     4681 tcctctgcgg tcgggaaggt ttactgtaat ctggcgatca aaacgtccgg cgcgaagcaa
     4741 cgcggggtca aggacatctt cgcggttggt cgcggcgatg acgatgatac cggagttatc
     4801 cgagaagcca tccatttcga cgagaagttg gttgagagtc tgctcgcgtt cgtcgttgcc
     4861 gccgccgagg ccggcgcctc tttgacgacc gacggcatcg agttcgtcga tgaagacgag
     4921 gcagggggcg gtctgtttgg ctttgcggaa catatcgcgg acgcggccgg cgccgacgcc
     4981 gacgaacatc tcgacaaagt cagaacctga aatcgagtag aaaggcacac cggcttcgcc
     5041 ggctacggcc tttgctaaaa gggttttacc ggttccgggt ggcccgacaa ggagcacacc
     5101 tttgggcagt ttagcaccga atttggcaaa tttgcgaggt tctttaagat attgaacaat
     5161 ctcgaccatc tccgcttttt cttcatcgca accagcgaca tcgctaaagc gaactttagt
     5221 gctgtcctct cgccgtgctc gtgagcgatt gaaatccatg gccttgctgt ttgagctgtt
     5281 aaccgaatta ttcagcctcg agaaaaagaa gagcgaaacg attactacgc caccgagaag
     5341 taaaatcgtc ggaccccaat tctgccacca gctgacttca aaggcatcgg tgtcaacaaa
     5401 gaaggcgccg ttatcggcgt tggggaaaag cgtattgtat tcttcgattt cggccttgaa
     5461 catagacgaa tagctggggt gatcatcggt ttcaccgaca acggcaaacg aattatgaaa
     5521 taactcgtta ggaatcatga cctcgtatga cccttctttg tttttggcgg ggtcatggta
     5581 aatgccactg actttcgtgg tttgatagcc cgaactgctc gtcgctgttt ttatgacata
     5641 tgagttaaga tttgtgtcta agtcgttgcc ggcggctgga ttgccggaca agtaccagac
     5701 ttcgggtttg ccttgcatgg atatgatgag ccacacaaaa gcaaagatgg ctacagccat
     5761 taacaagtat ggcagtatgt atccaaagat tgattttcta ggttttgctt cgttcataag
     5821 acctcctgag gcgatattcg ttttggtgat ttattttgac tgattggaca gtagtcggac
     5881 cgcaagtagc ataaattata aacaacgaac gtaattaatc ataacaaaat aattaaatat
     5941 tttgactggc atattttgaa taaacgcaag ccaaatgaag aaaggtagtt atactacctt
     6001 aatctcgaat ttggtcttct tatcaccggc aaaatctctc cgatatctcg ggacatatat
     6061 aattgtcccg cccttgttga cgacgagcgg ccaggtggcg cgcaagcgag ttggcatttt
     6121 ccagtcgata aaaaggcgcc tgacggcaca taaatgaccg ccgacttcgt atttgtcgcc
     6181 cggtttataa gttctgatcg tcagcggata atcttcatca tgaacatttc ggtcttcggc
     6241 gccgtggcta aagtcaaggg caaaataatc ggtttcgagg cttcccggtt tctctaaagt
     6301 aaaggaatac gaagcctgat ttgcgccact tagagataaa acatcgtatt ctttgaataa
     6361 agatatctca ttgcttaact ttatttcgat gttcggcgtt tgtgactgac aggctttttt
     6421 gatctcggcg atttgagcgg agctgatcgg aataaatttg cgtctgtttt tcaaaaactg
     6481 atcaattgct cgtgcaaaat ctttttcaga aagttcgagc agtgacttaa ttgacaattc
     6541 tttcttggct tgaacttttt tggcgacctc acttttttga gcgagaagca tatcgttatt
     6601 ttcgttgatt tccttgagga tttgatagcg ttcgatttcg gttaacctag cgacgacctg
     6661 aagtcttatt ttattgcgca aatacttggt ttcgaaattg ctcacgtcga ttgaaaaagg
     6721 cactctgttt tcatggcagt agtcgagcag gtcttgtttg gaaaaagaga gcaagggacg
     6781 gacaatcgtc atatttttca tcagaataac gggttcgatt ccgtaataat tcacacggct
     6841 gttgcgctgt ttttgaatca ggtaggtctc gattaaatcg tcttgctgat gagcgacaaa
     6901 caaggcgctg gccctgtgct tttcatacat atcggcaaag aattgataac ggagttcgcg
     6961 agcccaggat tgaaaattgc ccgtcgccgt tttcggagca tcgaatttct cgaaaccaag
     7021 gttgtgttca aggcagtaag cgcgcagaga ttcttcctca aagtcgctga tattgcgctt
     7081 gtgataattg acgtggcaaa cgacaatttt tgctgttttc tgcatcagca gatgaaggag
     7141 agccatcgaa tcgggaccat atgagcaggc cacgagatag gtagcgtttg tgtcaaacga
     7201 ataattgata atcatctatg atctcctttc caaaatattc tcttgataga gaatttcata
     7261 catatcttcg gcttcttgca aactggcgtg ttcgttgacc atcaggactc ttactccgat
     7321 cgtgttgcga ccgaggtgca tttcaatttc atcgccgact ttaacttcac ttgacggttt
     7381 ggcggttttt ccgttgatta aaattcttcc gccgtcggcg acatctttag cgacggaccg
     7441 tcgctttatt aaccgcgata cttttaggta acgatctaac ctcatatttt tattttacaa
     7501 cccctagtgt tttttaacac taacattata gagttcgccg atgatggtca aaagttcttc
     7561 caaatcattc atccagtcgc cgttcttggg catctttatc ttaagaattc tttgaatata
     7621 agagactttt acttttgtaa gatacggaac gattctctca aagagggcgt ttccaatacc
     7681 attaattgat gagaattctt ttgatagaac gatgttaatt tcatcgtcgt attccaaaat
     7741 ttctgaaaaa gcttttcctg atgccaacat atctatccgc ctctgcctga gcagcgtttc
     7801 gacttcgcgg ggaatgcgtc cataaacgtc gcgcattctc gcgctgactt tactcaaagt
     7861 ttcaagattt ttggctgact caatctcttg ataaagttca atcttgtctt tcttttcggc
     7921 gaaattggcc ggaatataag cttcgacatt aaaaagatgg gcggcgcgcg gctcttgaat
     7981 cggaacgccg gtcttcttct cttcgattgc ttccgagagc atttttaaat acaaatcgat
     8041 accgaccgaa tcaatgaagc cggcttgttc cggaccgaga atatcgcccg caccgcgaat
     8101 catcaaatcg cgttgggcaa tcttgtatcc gctgcccagt tccgtaaaat cctgaatcgc
     8161 ctttaggcgt ttcacggctt tatcgttgat cgttttatat tctcgataaa aaagataggc
     8221 ataggcgatg cgactgcccc gaccgacacg acctttaatt tgatagagtt gcgataaacc
     8281 gaaatgatcg gcatcctcga taattattaa gttagcgttg gcgatatcaa tgccgttttc
     8341 gataatcgat gtcgcgacga gaatgttcaa gtttccggca tagaaattca tcatcacatc
     8401 ttcgatttct tctcgtccca tctggccgtg gacaattccg attttcgccg cggaaatctt
     8461 cctttgcaaa cggttggcga cttgataaat cgatgagact ttgttgtgaa catagaagac
     8521 ttgaccatcg cggctgagtt cacgctcaat caactcttta atcacatcct cgcggtattg
     8581 catcacatag gtttgaatgg gcatgcggtt aatcggcgcg gtattaatct gcgaaagctg
     8641 gcgaattccc agcagtgata tttgcaatgt ccgcggaatg ggagtcgcgc ttaaagtcag
     8701 aacatcgaca tttatcttga tctcttttat gcgctctttt tgctcgacgc caaaacgctg
     8761 ttcttcatcg acaatcagaa gaccgagatc cttaaagacg atatctttgg acaaaaggcg
     8821 gtgcgtgccg atgacaagat tgacttctcc actctcaact tgtttgagat atttgcgctg
     8881 ctgcgcttcg gagatgagtc gagaaaaaac tgcaattcgc acaccaaaat ttgcaaaacg
     8941 ctcgatggcg cgttcataat gctgacgagc taataaggtt gtcggacaca aaatagccgc
     9001 ttgtttcccg tttgaaatag ccttaaatgc cgcccggaaa gcaacctcgg ttttgccaaa
     9061 cccgacatct ccgcagagga gccgatccat cgattcggac ttctccatgt cggccttaat
     9121 ttcttcgagc gcttcgcttt gatcagcggt aagttcaaag gggaattcgt cttcaaaaag
     9181 tttttggatt tcatcgtctt tcttaaaagg atatcccgga attcggattc tttccttata
     9241 caagacgacc agcctttctg ccagttcgtt aacacgtttt ttaatgcgcg atttggtttt
     9301 ttcccattcg tttccgccga gtcgatttaa ctttggcgtc gccccttctt ttccggcgta
     9361 tttacgtatt aaacggaact gcgaaagcgg tacgtacaag acatcggtac ccgcataagc
     9421 gatgtgcaaa aagtcgcgct tgttgccatc gatttccaaa gtgacgatgt cgatgaactg
     9481 accgatgcca ttatattcgt ggacgacata gtctccgctt tctagttcgt caaacgattt
     9541 taaaattgtc gattctttaa agcgattcat gaagcgcgaa cgatgcgttt tataccccag
     9601 taattctttg cttgataaat aacaaatcct ctcatcaacg agttcaaaac cttcgttgat
     9661 atcaaagagg gtcatcccca gcttgccttt gggaagctcc agggcttcga ccgtttcata
     9721 aactatgtcg gcatctttca cgaggctttt tgcggtatca aactgctgct tattagatag
     9781 ggcaagaacg accttttcat tggtggaaag ataagattga acggtaggca gtatattcat
     9841 ataagaggtg ctgctggaaa gaatcggtcg gactttaaat tcgatgtcat cgatactttc
     9901 cacatacttt ttactaaaaa gtggttttgg cagtttctta aaaatatcgt tccatgggcg
     9961 atagatggca aggtgcgaga taatctttcc ttcatcgacg agttcgtcta aaaatgcgcg
    10021 ggcttcattc tccgatattt ctaacgacgc cataatcgcc tcttcattga cagcaaagat
    10081 aatatcgaaa ttagcataat ccaagattga atagtgctcg cgttgagcat acccgtaata
    10141 cttataattg cgcgggtgaa tttgtccgct tttaattctt tcgaaatcct cttttgtcac
    10201 gctcattaaa tgttcttttt cgtcgtttga taaaagagtg gcgtctttta gcgcttgttg
    10261 ttcaatgttg acctcgagac gagatatttc ctcttccgag aagatgacat cgcttgccgg
    10321 agtaagtgaa atctcgctca gtctttcgtt tgatgtctgc gttgcgatat caaaggtgcg
    10381 aattgattca atttcatcat cgaaaaattc gatgcggacc ggaaaggggt tgttgacgct
    10441 aaaaatatct aggatgtcgc cgcgcgaggc aaattgcatg ctctgatcaa ttttaccgac
    10501 gcgggcgtaa ccgccgctgg ctaatttttg gcggagttca acgagattta ctttcattcc
    10561 aacccgtaaa ttaaagctca gactttcaaa gagctcagca gacggcataa accttgtaag
    10621 cgatgaagtg tgggcaatta atattttgtt cttgcttttc cttagttcgc tcatgacata
    10681 aaggcgctgc gccatcaact ctttcgaaga agaaattgtt tccgcccgca ataaatcgtc
    10741 gttagggaaa agcaaaacat tatcatctcc gatgagcgct cccaacagct cgtagacgct
    10801 ttgcgcatta taaagattgg atgtaataat cgcgtaattc gcgggttttt tgtggaaaag
    10861 tccagcaaaa agcaaggaaa cgccaattgt gtcatcaacg acaaaagacc ctcttttttc
    10921 aaggagggag gtggcggctt tggttttggc gattcgtttc agtaattcgc tcagatgagt
    10981 ttcctctagt tatattttgc catggccgat gtaaatccgt ttcgcagata atctttgatt
    11041 gcttcgctgg cttcaccgat ggcaatatcg attaattccg cttgtgcctt tgtcggtttg
    11101 ccaaggacat agtcaatgcc attgttttct ggctctccga ttcctattcg catgcgttta
    11161 atatcttcgg ttttgaaaac ttcgatgatg ttttgaatcc cgttatgacc accgcttgaa
    11221 cccgccggac gcaggcgaat tcttcccggt tccaatgcca tatcatcgta gatgattaga
    11281 atgtctggaa gttcaatttt aaaaaaagag gcgatttcct gaacggccgt tcccgacaaa
    11341 ttcatatagg ttgtaggctt gaacaaaaga acttgttctc caaagatgct ttctttagca
    11401 tagaaaccgt taaaagcctc tttatcgatg tcaaaaccga gcgaatcggc aaggcgatcg
    11461 acaacgcgat agccgaggtt gtggcgcgtc ttttcgtatt ttttgccggg atttcccagc
    11521 ccaacaatta atttcatatc gttagttcca ctcaaattat tataccaatc cttcgcgggc
    11581 ctgacatgca aaaatacttt gtttatacct tatcgctaac gattgttgta atatttattt
    11641 tgaaatatga agaaatacat taaagaaaaa ggcatcgacc tcatcaaagg tctggcgatt
    11701 ggcgtcgctt ttattatccc tggtgtaagc ggcggaacaa tggctgtttt attaaacctt
    11761 tacgataaaa tcatcaactc catcaataat ttgactaaga gatttgtcga ttcttttaaa
    11821 actttgttgc ccatcgctct tggagcggta ctggccatcc tcatatgctg gtatcctttt
    11881 aagctggcct ttgaacatat catgattgtc atggtgactt tgttcgccgg tttcatttta
    11941 ggcggtatgc ccggcatttt tgacgaagta aagggcatta aaattaaacc cgttcacatc
    12001 gtcgcaatga taatatcgat tgtttttgct gtcgcactag gagccttatc tgttctcttt
    12061 gagctcaaca ttcaatcact ttatgactct cgcccgtggt ggctctatat ccttatttta
    12121 ttagctggca tcatatcttc tttcgctttg gtcatgcccg gcatatccgg ctcgatgatt
    12181 ttaatcgtcc ttggtttcta cactccgaca ttaaatttaa ttgataactt tttggcctgg
    12241 actgatgtcg gagcttcaat ctcgattctc gccacgatgg ccgtcggcgt cgttatcggc
    12301 gttcttctgg cttcgaaaat catcgccttt tttctcagcc gtttccggac cggcaccttt
    12361 tacgccatca tcggcattat catcggatcc ttaatcgcaa tttactacaa ttacgaaatc
    12421 tatgattatt acttaaccgt cggcgtccgc tggtgggaaa ttctgattgc cggcgtcgcc
    12481 cttgtcgccg gaattgccgt ttcatacatg attgtccgct acagccgcaa ccataaaaca
    12541 attaaggagg ttagtaatta atgagtttgc tatccattgt tctaagcgaa gctattgaag
    12601 gcttagaaaa cgtcggccga ttctgggatt taggaacttg ggaaatgacc tttctcctct
    12661 ggctcaacag cctgcatggt actttcatcg acaaagtctt tctagtcatt accatgctgg
    12721 gcgacaaagg catcgtctgg atcacctccg gaatcgtgat gctcttcttt aaaaagacga
    12781 gaagaatcgg catatttgtc attttgacat acggcttaat cggcggcatg aacaacttcg
    12841 tcattaagaa aatcgccgtt cgcgcccgcc ctttttatga tacgacaatc gccgtttatc
    12901 ccagcgccca cgtgctgcaa gagtacgctg ttgccacttt ctttggcgat ggtctctttc
    12961 tcggatttgg cgaaattccc gatgattatt ccttcttctc gggccacacc gtcgcttcaa
    13021 ttgcctgtgc gactatgatg attcttcaca gtcgcaaaat cggcattccc gccgtcatcc
    13081 tggctgtatt aattgccttt agccggctct ggcacggcgt ccactggccc actgatgtgn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtattaattg cctttagccg
    13261 gctctggcac ggcgtccact ggcccactga tgtgcttttt ggaactatct tcgcggtcgc
    13321 catcaccatc ggcttgttct acttgcttcg tttcctcgaa ccaaaagtaa tgaatctgct
    13381 gaccaaaatt ttcaaaaaga acgaccggtc agccatcgaa caataacatt aataagcaaa
    13441 ttagagcaca tagtactctt ttttgtttag gtaattttgc tttcaaagac cttgaaaagg
    13501 gttgttaaca agtctatact ttttacattg acaacaatgt caatcaaaag aaatatgcca
    13561 aaaaccccag aacaaaatca actgattaaa gacgagagaa agacgcgcat tctccatacc
    13621 gccttgcgcc tttttgcctt gcggggctat gattcggtcg taattaacga catcacgaaa
    13681 gaagcggaat gttcgcatgg ccttttctat cactatttta aaaacaaagc cgaagtattt
    13741 cttgaattga tgaaaatcgc tgaagagcgg gcctcaaaaa agcgcgaaga agaaaaagac
    13801 tttaataaag tcaccgccat caccgccatt cgcgccatcg tctcccgcat gcttgacgaa
    13861 atctatcgcg aagatgaatc accgtacttc ttatatatgt ttgtgaatat gcatttgcag
    13921 cgaactctgc ccctcccccc taaaagcaaa ataccgggac cgaataaacc gttttttcac
    13981 ttcttcgtcg acctgattgc ccgcgggcaa aaagaaggcg acgtctccgg gggcgatccc
    14041 aaagaatttg ccatcatcta ctactccatc atcaaaggtt tatgctacac gcggctaagc
    14101 atgattaaca agtcgccgac taaagtcagc ccggacatca tcatgaattt gtttaccaga
    14161 aaggggaact actaatgctc aaattattcc gttttttaaa gccaaaattc tggctcataa
    14221 taataatttt cctaatgatt ggggctcagt ctttctcgca gttgatgctt ccgagcctga
    14281 tgggtgaaat cgtcgccaat atcaccggcc cgacgggagc cgataacagt tttattatcc
    14341 gcaaagggct tgagatgata gcctatgccg gggcgacgat catcatcgct tttgcaacct
    14401 cgatcggcat ctcttatgtc ggggcgtatt ttggcaaaaa actgcgtgcc gaagtgttca
    14461 aaaaagtctt gagcttttcg cctggtgact atgaccgcat cggcaccgca tctttgatga
    14521 caagaacgac aaatgatgtc gagatggtcc agaccgtcgt cgtcatgggt ttaagaatca
    14581 tggtaatgtc acccatcatc atggccatcg ccatcgtcca agtgctgaga accgatgtcc
    14641 agctggctat catcctcgca ttttctattc ccttgattat tattaccatc attattctct
    14701 tcgcgattgc catgccgcta tttaagaagt tacaaatcgc catcgatgaa gtgacaaagg
    14761 ttttgcgcgc ctcccttacc ggcgtccgcg tcgtccgcgc ttttaaccag gaagaaaaag
    14821 aaaaagagcg tttcgccaaa gttaaccgct atcaggccaa tttgatcatc aaagtgggtc
    14881 ggacgatgtc gtttgccagt cccctcatct ccattttctt tgatttaacc tacttcgcga
    14941 tatttttgtt cggctttgcc attatcgacg gaacggcaaa tgatgcgcgg cttgataaca
    15001 ttctcgtcac ttcccaatat gctatgcata ttatgatgtc atttttaatg ttttcgatgc
    15061 ttttaatcaa cattccgcgc gccggcgtca gcgctcgccg catcaatgaa gtactcgaaa
    15121 ccaaggtcga tatcgtcgat ccagaagttc ctcattccga aaataaaggt gaagtcggtc
    15181 atgttcgatt cgaagatgtg acttttactt tcccggattc gaagataccc acactccaaa
    15241 acataacttt tgacgccaaa cccggccagg tgaccgccat catcggttcg acggggagcg
    15301 gcaagtcgtc aatcatcaat ctcatcccgc gcttttatga tgtgtccaaa ggacgcgtgt
    15361 tagtcgataa tgtcgatgtc cgcgattata cccaaaagga tttgcgcgat aaaattggct
    15421 ttgtacctca gcaggccctt cttttttcgg gaacgatccg cgataacatg catttcggaa
    15481 aaccagatgg tagcgacgag gaaatcaggg ctgctctcga agtcgctcag gcggctcgtt
    15541 tcgtggctcg caaagaaaat ggctatgacg ccgaagtttc ccaaggtggc aaaaactttt
    15601 ccggaggcca gaaacagcgg ctcgccatcg cccgcgccct ggtcaaaaaa ccggagatct
    15661 acatctttga cgattcgttc tcggcacttg atttcaagac cgacatccgc cttcgcacgg
    15721 ctttaaaaga atatacgaaa accgcttcgg ttatcatcgt cgcgcaacgc gtctcatcca
    15781 ttatggatgc cgacaatatc ttagtcctca atgaaggcaa aattgtcggg cagggcaagc
    15841 atcaggaact attaaaaaaa tgccaagtat atcaggaaat tgtcttctcg cagatggatg
    15901 agagcgaaat taaacgaact cttgaaatca agaagcaagt tcttctttcg gaaggaggtg
    15961 acgaataatg cctcgttcaa ccgatgccat gatgggcgct aaacccaaaa acctcaaaaa
    16021 ggccgcatcg cgcatcctcg ccgattttcg accgctctta ggagtcttca tcttcgtcat
    16081 tattatcctt ctagcttcgg ccgctctttc ggttgtaatt ccggttctct tgggcgcttt
    16141 cgtcaacaat tttaaaaacc tcgccaccaa agtggaaggc gtttatcaag tcgattggcc
    16201 ggaagtgttc aaacaagtca taatcatgct gtctttctat gtcggctcgg ccttattcat
    16261 atggctcgcc gactggatcg tcgtcaaaat ctccgctgat tacgcctttg atatccgagc
    16321 aaaaatcaag gctaagctcg atcgtctacc gctgagctat tttgataaaa atacttacgg
    16381 cgagatttta tctcgaggca ccaatgacgt cgacaatatt tcccgtcaca tgagcagcgt
    16441 aataaaccag acgatcatcg ccgtctcgat gtttctcgga acagttatcg ccatgttcat
    16501 cacttcctgg caactcgctc tcgtggccat cgccatcttg cctttcacga ttctcttcac
    16561 tgtcttaatc gctgtcaatg cccaaaaaga gtttaagaaa tatcgctcgc atctcggcaa
    16621 gttaaacgcg atcaccgaag aaaacttttc gggttttaaa gtcgtcaaac tctttaacaa
    16681 agaaaaagat gtggccgcgg cttttgaagg cgtcaatgaa atcatgatga aagctgactg
    16741 gaaatcgcag tggctgtcgg gctttatctt tccgacgatg cgctttatcg gcaatctcgg
    16801 cttcatcggc gtcagcgtcg tcggcggtct tttgcaagat cccggcaaca tggttgcttt
    16861 cttcctgttc ctgcagatgt tccagcagcc ctttcaacaa atcggtcaga tctcgagcat
    16921 cattcaatcg gtgctggctt ccggcgagcg catttacaac cttctcgatg aagaggagat
    16981 ggaatctgaa gaagcgggtg cgctttcgac cgaagatgcc atcaagggcg aaattgtctt
    17041 tgataatgtc gaattctctt atagcccgga taaaccatta attcaaaact ttaacttaaa
    17101 agttaacatt ggcgacagca tcgcgattgt cggcccgacg ggcgccggca aaacgacgat
    17161 cgtcaacctc atcatgcgct tctatgaagt caatagcggg cgcatcttgc tcgatggcgt
    17221 taatattcgc gactatcagc gcgcgacatt gcgcggagcg attggcatgg tcttacaaga
    17281 cacctggctt ttcgcggatt caattcgtga aaatatccgc tacggacgca gtgatgccac
    17341 cgatgcggaa atcgaagagg cggccaagca agcgcatgcc gaccatttca ttaagactct
    17401 gccgggaggt tacaattttg tcctcaatga ggacggaaca aatatttcgc aaggccagcg
    17461 tcaactaatt accattgctc gcgcgattgt ctcaaagccc aaaatactca ttctcgatga
    17521 ggcgacatca agcgtcgata cgcgcaccga actagcgatt caggacgcga tgaataagat
    17581 gatgatcggc aagaccagct ttatcatcgc ccaccgcctt tcgacgatta aaaacgccaa
    17641 ggtgatcatc gtgatgcaaa aggggcagat tgtcgagatt ggaaatcatc aacaactctt
    17701 ggccaataac ggattctacg ctgaacttta caacgctcag ttctccggcc gcaatcccct
    17761 cgctgaaccc gaacaagaaa gcgataatag cgaggcaaaa gcggcagtct aagaaaacaa
    17821 aacgccttct ctgcggggag gcgtttttat taacagcgca aaaacacatt ccgcaattta
    17881 tgataaaatt gctttacgag aaatcatggc ccattccatc gcagtaagta ttattattcc
    17941 cgttcgaaat tccgaaaaat atctttcgtc ttgtcttttc agcgccgttt cgcaggattt
    18001 tttgttgccc tatgaagtaa ttatcatcaa caacggatcg accgataaca gccgcgaaat
    18061 catcgataaa tacgcgaaaa aatataagtt cgtccgccag ataatcaccg atggcctttc
    18121 cgtcggtttt gcccgcaagc gcggcattga agaagccaaa ggccaataca tttgctttct
    18181 tgatagcgac gacatgtata agctttcgtt tgtcaaaaaa atgtatgaga cgataaccgc
    18241 aaacaatgtt gatatcgtca attgctcgta tgtcaaaatc aatcctaatg gccgcatcag
    18301 ccgcaatgtt ctcgctcgca agcgctcatt agattgcata cacggcgtgc gctcgctgtt
    18361 gcgcgatttc aatatccgcg gatatatgcc gatgaagatg taccgagcgg acctgatcaa
    18421 aaataccaaa ctacccatct cgagcaaatt gattttgttc gaagattttt taatcaactt
    18481 tgctctctac agcaaagcca gtaagacaac aacaataact gaaccgcttt atttctaccg
    18541 tcgcacgaat gagtcggcga caacgaacct ttccagcaat cgtaccgacc tgcacctcaa
    18601 atgtttcgcg gccgtccgct ttctggccga acaaacgggc gataaagccg tcatcgacga
    18661 gttctttaaa cgcggtttcc gctactggct gtcgatcttt gccgatgtct ttttatcgat
    18721 caaagttaat gagagaccgt ttttcgtcga gcttcaccgc gccgaaaaaa ttaagcgtgt
    18781 catcctgtcc cgcaaacccc ttccgatcaa aggcatgctt tgggaacaat taatcaaaga
    18841 aatcagttga ggccgtttta tctaagataa aaacgcttac ataagcgatt ttagttattt
    18901 ttgtccaata tctatgatat tcttgtatcg gtgagcgaag acagctcata aattataaaa
    18961 aaggagaata aaatggcaaa aaaatacgtc tacaatttta aagaagccca tggtcttgga
    19021 aaagaactcc ttggaggtaa aggtgccggt ctcgcagaga tgactcatat cggcatttcc
    19081 atcccccaag gttttactgt ttcgaccgaa gcttgcacgc tttactacaa cagcggcaaa
    19141 atccttcctg actacttagt taaggaaatc tacgatgcga ttcacggcat cgaaaaagaa
    19201 accggcaaga atttcggcgg ttcccataat ccgttgctcg tctccgtccg ttccggcgct
    19261 cgcgccagca tgcccggcat gatggatacc atcttaaacc ttggtttaaa tgataacacc
    19321 gtccaggcga tggccaatga aaccagcaat gaaagatttg cttatgactg ctaccgccgc
    19381 ttcatcgtaa tgtttgccaa tatcgcgaag ggccaccccc ggaccgagat ggataagatg
    19441 ctcgaggagt taaaagagca cagaggctat cgtcttgaca ccgagatcac cgctgatgag
    19501 ctcaaagaac tcgtcaagaa atataaagcc tattataaga aggtttttgg cgaagatttc
    19561 ccgagcgatc cttatcagca ggcgatcgaa gccatcaccg ccgtcttccg ttcctgggac
    19621 aatccgcgcg ccaatgtcta tcgcaagatg aataatattc cttacgattg gggcacagcc
    19681 gtcaacgtcc agtcgatggt cttcggcaat aaaaacgaaa acagcggcac cggcgtcgcc
    19741 ttctcgcgtt cgccatccac aggcgaaaag aaagtttttg ccgaatattt gcccaatgct
    19801 cagggtgaag acgtcgtcgc gggtctccgc actccgcttc acatcgaaga gctcaatacc
    19861 agaatgcctg aagtctacaa ggaattcgtt tcgacgattg gcagaatgga aaaccattat
    19921 cacgatatgc aggatatgga gttcaccgtc gaagacggca aactatattt cctccaaacc
    19981 cgcagcggga agagaacggc ccaggccgcg ctcaaaatcg cttgcgattt agtcgatgaa
    20041 ggcatcatct ccgaagacga agccgtctta agagttgatc ccaacattct cgatactctt
    20101 ctccacccga gtttcgataa aaaagaactc gccaaaaccg aaccgatcgc cgttggctta
    20161 cccgcctctc caggtgccgg tacgggtaaa ttagccttta ccgcccaaga agcaaaagcc
    20221 cgccacgaag ctggcgaaaa agtcattctc gtgcgcattg aaacgtctcc tgaagatatc
    20281 gaaggcatgg tcgcagccga aggcattctc acgatgcgcg gcggcatgac ctcgcacgcg
    20341 gccgtcgtcg ctcgcggtat gggtaaaacc tgcgtcgccg gcgcttccgg catcatcgtc
    20401 gatgaagaga agaaaacggc taaaatcggc aaaaagattt acggcgtcaa cgatgttttc
    20461 tccctcgatg gctcgaccgg taatgtctac gaaggcgcaa tcaagactgt cgaagcccaa
    20521 ctgactggct attttggccg gattatgaaa tgggccgacg aacgccgcac gctccacgtt
    20581 cgcgccaatg cagaaacacc gcgcgatgcc cagaccgctc acaatttcgg ggccgaaggc
    20641 atcggcttag cccgcaccga acatatgttc tttgataaag accgcatctt cttcatgaga
    20701 aagatgattc tcgaagaaac cgtcgaaggt cgtgaagagg ctctggccga acttctcccc
    20761 tatcaacgcg acgacttcta taaattattc acgattatgg gcgaactaaa cgttaacgtc
    20821 cgccttcttg atcctccgct tcacgagttc cttccacagg aagaagacaa gattgaagaa
    20881 ttggccaaag aacttgataa gactgtcgac gaaatcaaag cccgcatcga tgatctccac
    20941 gagttcaacc cgatgatggg acaccgtggc tgccgccttg acgtctccta tccggaaatc
    21001 ggcagaatgc agaccagagc gattatcgaa gctgctttac aagcccaaaa agataccggt
    21061 aagaagatca ctcccgaaat catgattccg ttgacgctcg acgccaagga atttgaattc
    21121 gtcaaaaaga tcatcaccga aacggccgat gagatcatcg ccgcttccgg tcagaagctc
    21181 gtctattacg tcggcacgat gatcgaaatc ccgcgcgctg ccttgctcgc cggcgaaatc
    21241 gccaaaggct cagctttctt cagctttggc accaacgacc tcacccagat gacttacggt
    21301 ttctcaagag atgatgccgg caagttctta aatgattact atgcgaagaa aattttcgaa
    21361 caagatccct tccgcaccgt cgaccaacgc ggcgttggac aattgattaa gatgtcggta
    21421 gaaaacggcc gcaaagccaa tcccgacctc cacatcggtg tctgcggtga acacggcgga
    21481 gaccccgtct cagtcgaatt cttccacaac gccggtcttg attacgtcag ctgctcgccc
    21541 ttccgcgttc ctgtcgcccg cctggctgcc gcgcaagccg ctatcaaagc caaagccgcc
    21601 aagaaataat tcttgacgcg cgaataagca aaacaaaaac gccattcaca tttaatcgtg
    21661 atggcgtttt tttaatctta cttcttttgg ctggcgagct ttttctgaag agctctttct
    21721 cttacaacaa taccataacc cggtttattt tcctgacggg cgcgggcgat agccatatca
    21781 tctttgggaa cgctgcgatt aatcgttgag ccggcggcga taaaagcatt atcctcaacg
    21841 acaatcggcg agatcaacgt gcttccggag ccgacaaaaa catcttttcc gataaccgtc
    21901 ttggttttat tgtaaccgtc gtaattcgca acgattgtgc cgcagccgat attggttttt
    21961 tcgccgatat cgacatctcc gagatagctg aggtgcgccg ctttggctcc atcgtgaagg
    22021 atgcaggctt taaattcgac gaaattaccg acgcgggtat ggttgccgat ggtggaatgg
    22081 ccgcggatgc gggcatatgg accaacttcg ttcgcgcttc cgatgttcga atcggcaacg
    22141 tgggagaaaa tgatgtgatt ttcatcaccg atgacgacat tatcaaggaa agtggaggga
    22201 ccgatgacgt tttgccggcc gatcgccgtc tttccataga tgtgagtgcc tgggcgaatg
    22261 acggtgtcgg gttggatgac gacatcaggt ccgatatacg ttgaagatga atcctcaatc
    22321 gtcacaccgg cgagcatgtg ccgctcattg atttcgcggc gcatgatggc ggccgcttct
    22381 tcgagttggc ggcgatcatt aatgccgagc atctcccgat aatctccagc ggtagaggcg
    22441 ccgattttta agccgtcgtt tttaaacatt tttatcagat cagtgagata gtattcgcct
    22501 tgagcgttgg ttgggcttaa tttgtccaaa tattcaaata agagtttatt gtcaaagaca
    22561 taaacgccgg cgttaacttc gttaatggcg ttttgctttt cattgacatc agcttgctcg
    22621 acgatttctg aaacgctgcc gtcgcttccg cggataatac gtccgtaacc gcgagggtta
    22681 gggacgatgg cggacaggac tgtcaatttg ttgttctctt gatcgtgctg ttcaatcagt
    22741 ttttgaagag ttgtcgtttt taatagcggc gtgtcgccgc agacgatgag ggtgcggcct
    22801 tgctccccgg ccagcaaggg tgcggcttgc atgatggcgt ggcctgtccc tttttgttcg
    22861 tgctgataga cggtttccgc ttcatcgcgg acgatttctt cggtcgcggg tccgccgaaa
    22921 ccgacaatcg cgactttctt ttcgatattc aaaggccgaa gagcgttaag gacgtgcttg
    22981 accaaaggta cgccgaggat gggataacta actttggatt tgtcatcgag aagagatttc
    23041 attctcgtgc ccttgccggc ggccagaata atcgcgtaag ttttcatact atttgctcct
    23101 taaagtgcgg gttaatcgcg tatcataacc catttttgcg tacttttttt ctaatttagt
    23161 aagtttttta gcatcttctt cgagggcgat aacagaacta cctgatccgc tcattaacac
    23221 aatttttatg ccatctttta acagcgaatc tttaatttta gcaatttctg gaagcattga
    23281 aaaagatggt ttttctaagg cattatgtac tgatttggcg agcaaatcgt cgtcgccttt
    23341 agctaaggct tcaatgacat tatcgatgtt tccgttttcg agttccatac cgtcggcccg
    23401 atcaaagaca tttttggtag aaagaccttt aaggggcttg acgataagca cgtgatagcc
    23461 ttttttgatt ttgatcggct cgataatttc accgatgccg cggacgcgag acggaatgtt
    23521 aaaaaggcag taaggaacgt cggcgccgat cgacttgccg agctcgatcg tttcatcgcg
    23581 ggagggcttg atctttagaa gctggaaaag agcgttcaaa acggcagccg cgtttgcgct
    23641 tcctccggct aggccggccg aaatcgggat gtctttatag acatgaattg taaattgttg
    23701 tttaaattta tatttctcac gagctttgtt aatcgcgatg gtaatcagat tatattttgt
    23761 ttcttcaatt tcaatatcgt cacaagtgat gaaggaatcg gaagaatcag gcaagacgga
    23821 gatttcgatg gagtcgtgta gctccagcgg caaattgacc atatcgagtt ggtgataccc
    23881 attatcgtcc ttgccaagga catttaaggc aatgttgatt tttgcgtaag ctttgatata
    23941 catttgggct tccttttggc gatatacatt gtatctttga agtattagaa cttcaattgt
    24001 tttatcaggg ttaagtagaa gtctaaattg agctcttccg gcctttttga gccgtcgacg
    24061 cccagctcac tgagaacttt gtcggctttt tcgatgtcat ctagataaat tcgcaggttg
    24121 ttgcgaacgg ttttgcgacg gtgatggaaa aggctgttgg ttatctttac aagtttctcg
    24181 gctgtttcat aatcgttgtt ttcatgaagg acgatttcga tgacgaccga atccactttg
    24241 ggagcgggaa caaaattgtt tttggcgaca ttaaagacgc gttttgtttt tccaaggaac
    24301 gacaaaagaa gagctaaggg gccatacccg tcttcgccaa aacgagcaat caaccgcggc
    24361 aaaacttctt tttgaatcat gaaaatcatg gtcttggcct ggcgggcatt gagcaggcaa
    24421 tattgaattg tgtcgctggt gatatagtag gggagattac cgataatttt gtggtattga
    24481 gaaacgtcat gttttagaat gctttcctgg actattttca cgtttgtttt ctgatcgaat
    24541 tgttcggtga gaaagctgat tattttcgga tcgacatcaa gcagcgttat ttcgccctca
    24601 cccaaagaaa taaaatacga aagagagcca aatccagcgc ctatttccag gaccctatca
    24661 tctttatgaa tctttgcttt ggcgacaatg ttttcggcga tatattcgtt aaccagaaaa
    24721 ttctgaccga gttcctttga ggcaataaag cgcagagttt tgataaaatc aaaaaacgct
    24781 tgctgactag cgaatttcac gaactgcctc ctctaattgc tgatagctaa ggttaagcga
    24841 ccgcgctctt ttaagcaact gcttgccgtt cacataaccg aggtgaaagc ggtcggagat
    24901 ccggatgcgt atcttcgacg aattgggggt gccgaccaag cccaatttga agaggtcaat
    24961 ttcccgcaaa tcgcttttca tcgaatgact ggtcaccaat tgaaaatcaa gcgccttgag
    25021 aatagttttt cgctcggctt cagcaacgcc gactttgcca ttttttacgg cgtctttttt
    25081 gttaatgaaa caatgccgca ggtcaggtat gttcgcgtct aatttagtcc tgatcatctg
    25141 gcccggggtg tcgggatcgg tgagaacgac aatcggttta atatggcttt gttctttgag
    25201 gtaggcaatc gttgtatcgg ggacatcgga accgtttgtc gaaacaaact cggcatcgat
    25261 aaatgaattc agaaaagcaa tatcggttat accttcaacg acgataatgg cgtcaatttt
    25321 tttcttcatt gtttttcgag cctgaataaa cgacggaaat tcgccgttgt cgcctcatca
    25381 ataatcttgg cctcaacacc tctgaggcgc gcgatttctt caaggattag cggcagattt
    25441 gcgggactat tagtctttcc gcgaagagga tgcggggata aataaggcga gtcggtctcg
    25501 actaaaagtc gattcaaggg ggtgattttt gcaacttctt taggtgtttt cgcgttcaaa
    25561 aaagtaaccg gaccgcccaa agaaatatat aatccgagat caacataatc gccaaccatc
    25621 tcggcacttc cggagtaaca atgcatgatg ccgccatatt gaggtggatt atcgcttaaa
    25681 atcttgaaag tatcataagc ggcttcacgc gtgtggacga taatcggctt ttggtgttcg
    25741 ttggctattt tgatttgttc gataaaatac tctttttgtt ccttttgatc ttcttcgctt
    25801 ttatcccaat aataatcaag accaatttca ccatatccaa taactttttt gtcgctgagc
    25861 atcgcctcga tttggccgag atcagcgggc ccgcgttttt ttatatctgt cggatgaatc
    25921 cccaccgcgg catagacgtt ttcgaagcgc tgagctagag catatgctct tcttgaagat
    25981 tcgaggtcgt aaccgatgac aagaaaggct tcgacgcctt ttgccatcgc ctcttcgatg
    26041 tatttttgcg gatttgcgta aaaagaatta tgattaaggt ggcagtgcga gtcaatcatg
    26101 gttactttcc gacacaaaaa cgcgaaaaaa tctcttcgct gatgtcgttt ttgttccctt
    26161 ccccaagaat ttccaaagtg gcgttatagg cgctgagcaa attgacgctg accaaatcga
    26221 tcggctgatt atctttggcg tcttcaatcg cttgatatag actttcgcga atcgcgccca
    26281 gtaaaccgag ctggcgggtg ttatttaagc ttggctgatc ataggcttct tggctaattg
    26341 acagagcgtc cgtgatttcc tttaagagtg gatcgatatc cttgttcaag gccgagatat
    26401 ataaagtgtt ttgttttttg tctttggcta agtcgctttt attgtaaaca ataatgtgtt
    26461 ttttgttttt gacaagcgcc agaaacgaat cgtcgtcgct ttgactcgcg tcgcgtacga
    26521 gaataaccaa gtcggcgtcc gctgaggcgg ctttagcttt tttaatgccg atggcttcga
    26581 tttcattgct gtcttcacga attccggcgg tgtcgagaat atgaagtgtg attcctttta
    26641 aattaatctc gccttcgacg atgtctcgcg tcgttccggg aatgttggtc acaatcgctt
    26701 tttcttgtcc gatcatggcg ttgaggagcg aggattttcc gacattaggc ttgccgataa
    26761 ttgcgacttt tataccttct ttgataatct gtccttgttt gccttcggct attaatttgg
    26821 taacgttgtc gatgatccgc tctgccacta aaatgacttg cgcgcggctc acttcttcaa
    26881 tgtcttcgta ttcaggaaaa tcaatattta cttcaatttg acttaataag tccgctattt
    26941 cttttttaat tggagcgacc aaattactag tttcaccttt tagtgaatag agagaaattt
    27001 tcttggcttc atgggttttg gcattgatca aatcatcgat ggcctccgct tgtagcaggt
    27061 caattttccc gttcaaaaag gcgcgggagg aaaactcgcc gtttaatgcc aagcgtgccc
    27121 cggaagagat gagcagttcg atgatttcat cgacgatcaa catcgagcca tggctgataa
    27181 tttccacggc gttttcgccg gtatatgatt taggatttac ataagcaatt aaaacgacct
    27241 catcgatgat ttcatcgttt ttcaaaatat ggccgacaag gacgttccgc ttggtggtat
    27301 ttcgcaaatc gtggctaaag actttggaga caacctcaaa acaatcatcg cccgacaagc
    27361 gaatgatagc taatgcggat ttgagcggtg gtgtcgccaa agcgacaatt gtttcgtgca
    27421 tctttttcct taatggcaaa aaagagccat aaggctctta attaatcgac gtatttaatg
    27481 acgacggcgc ggtgatgacc ttcgccggcg gattcggtct tgatgtgaga gaagttggcc
    27541 agggcattat gaacgactct tctttcgtcg gccggcatcg gctctaatgt tgcgtccatc
    27601 ttcgttttct gaacttcctt ggcagccttg cgggcggcaa aggcgacgcg ggagtattta
    27661 ccgtctttat aatcgttgat atcaaggaga attctgaatc tcttcttgaa ttttgatgag
    27721 actgcgagtc ttgctagttc attgagagcc tgcagggttt tgccgttttt accgatcaaa
    27781 atcgggttgt ggtctgagtt tagcgagatg tgaatgatgt catcttttaa gacggtggtc
    27841 gtctcggact gaatgccgag ggcggccgtg acgtttttta gatactcttc ggcaaattca
    27901 atcgcgtcgc ttaattcata aacttcaatc gtcacttttt tgaagaggcc ctttttctct
    27961 tcctgaaccg cgaagttgac atcactaggc aatatgccta attcttgagc cgccatttcg
    28021 agggcttcat caattgtttt ggcttgatat tttttcatag aaaactcctt tattttttgc
    28081 tgcgcgccat aattgtctgc atgattaagg tttgaatgac ggagatgatg gcgccgataa
    28141 accagtaaac acccattgcg gccggcaagc taaagcccat cacgacaatc atgaccagca
    28201 tgatattgct gaaccacttc atttgcttag actgcttatc ttgcgccggg ttcttcgaaa
    28261 gctttttgac gtttttggtt cgggctttct gcatccattg cggcagtttc atcgaaatga
    28321 attgcgagat acccatcaaa acaaataaga cgacggccgt ccaccacccg gcggcattgg
    28381 gccaaccggc gatattgaga atcgaggttc ccaaaccagc gcttaggttt aaacctaaaa
    28441 ccgcatcaga agaaagaacg gcggcaccgg tcatggcacc ccagacggcg ataaagaccg
    28501 ggaactgaac aatcaaaaca agaatttgac cgagtgggtt aatcttgttt ttcttgtaaa
    28561 gcgccatctg ctcctgggcg aggcgctgct tctcggcctg attggtgttg gagttcggat
    28621 atttggcctg aatcttggcc agttcgggtt gcagggcctg cattttttgc tgacccatgg
    28681 ttgacttaat ggttgtcaga agaatcaggg atctgacgat gagtgtcacg agggcgaccg
    28741 ccccgatttg accccagccg ttcattccga aagtaaaaga gaaataatcg accaagaaag
    28801 cgacgggata aaccaataat ccttcaattg gtcctttaga ccaggcatag gcccaactct
    28861 tgccttcaat aaaggcttcg gtgttaccgt cgcttccgta ttttccgtag ttgccgttat
    28921 taacagctat acaggcgcga taggtggaga tggtggtgtt cagtttctct ttatagagtt
    28981 tagtaaagtc ctggttgggc acttgttcgg cgccgaggct gacgcgaagt tcggcagtcc
    29041 agctgtccca ggtttcaaaa agattgttgt cctcggaata gccggcaaat ttcaaatagc
    29101 caaatctggt caaagcgaga ttggcttgtt cggcgttcac ggttgtgaaa tcaacactta
    29161 gtccaaattg tgaagttaaa gcaacctcca aaacttcctg atcgatggcg acaaaatacg
    29221 cctctgtggg aacattaatg gcgttcgtgc ccgcttcatc gatcacggtt tgcagaaggg
    29281 cgctgttttg atacttatct tcttctttcg gcacgcgtct tgttaaagtg gcgttgccgg
    29341 caaacacttc gactccgccg acggcgttat cttcatagac agaaacgcct ttttcatagg
    29401 tgtagaggat gttggcgcgg tcgttgttcg agcagaagct ggcggtacag ccgctgatga
    29461 ggacaacagc cataaaaagg ccgccaaagg taaggatgcg cttagttgat tttttcaaaa
    29521 ttatggtctc cgatcttttc aagaagaccc tttaaggcgt cgtgactctt gttgaattct
    29581 tcgacgttat aattgtgttt aggaacaatt acatagtcga ttgatgtact gacgtcgagg
    29641 gtctctcgaa gaatggcttt gatttgtcgc ttgattttgt tgcgggtgac ggcgatgccg
    29701 atctttttag gaatcgagat gccgacccgg acagcgttta agttgttggg ctgaaaataa
    29761 actacaaaga gctggttttt gacaaaacga gaagcgtgga tgatgcggtg gaagtcctcc
    29821 gatttgcgca atcggtactg ccttttcaca aatctctccg atgtctggtc aaaaccaaaa
    29881 cacttaggcg gataggaccg ctcttccttt ttcgcgacgt ctggcaaggg ttttacggcc
    29941 gtttggggtt ttcattcttt cccgaaaacc atggacggct gagtgctttt tcttacttgg
    30001 ttgatatgtt cttttcataa tctaatctcc tcgtgtgtga ttacgcgaaa tgattatata
    30061 gtttaatgta tgctaagtca aataaataag ggcgcctgcg catacaactt aaatgctcgc
    30121 gcaaagcctt ctccacaaaa gtcgataaat tcttgactga tataaagaat ggcgatgtcg
    30181 ccctgaagcg ttaaccacag gctgtgaatt aatagtatta ttacggtggt aaattacgaa
    30241 atatcaagaa ttagtcggtg ttttccagtg tgataaaaag gcgtgatccg tggatttcaa
    30301 tcaacaaaaa ccacagtcag atatccacac ttcgatgtcc acaagttttc cacaatccaa
    30361 ttttgtggag agagaaaatt aagcgtctta tcgggtgttt ttatgctcaa aatgtggaaa
    30421 actaaatatt cacttctttt cgggtgtttt taacctgtgg atatgttata tttttatgta
    30481 cttggagggt tcaagtatga ccagctcaat atcagaaatc agcaaattat gggatcgaat
    30541 cctcgacagc accaaaagtc gtattaacga cccgcgtatc tttgatacct ttttaaatag
    30601 ttcctacatc aacagcatag acggagacac gatggtcgtc gtcgtcaact cgggattagc
    30661 ggctaatata ttgtcgacta aatatttaga tatgattacc ggtgtcgtcc gcgaatgtac
    30721 agaaaccgat ttcaagctca agttcgtcca ggccagcgag gttcagacgg tggccaagca
    30781 agcggcaact aagccgactt tttttgccaa ttcatacatt aattccaaac tcactttctc
    30841 aaattttgtg gttggctctt cgaatttaga ggcttatcag gcatcgatcg ccatcgcttc
    30901 atccctcggc aaattattta acccgctttt catttattcg caatccggac ttggcaaaac
    30961 ccacctgctt cacgccattg gtaattatat aaaagaaaac gcgcccgtaa gtaaagttct
    31021 ctatctttca accgaggatt ttttcgaaga attcgtcaaa tatgtcaccg gcgacaaaga
    31081 aggagaaaac cttaaggatt tctttaaagg aattgatgtt ttattaattg atgacattca
    31141 gtttttagcc aacaaaatca agaccgaaga gatgttcttt tatatcttct cctcgatgat
    31201 taatgccggc aaacaaatcg ttcttaccag cgataaacac ccgagcgaac tgcagggagt
    31261 cgaagctcgt ctggtgtcgc gctttaacgc cggtcttacc gtcaacatcg ccacccccga
    31321 tgtgaagacc tgcgttgata tattgaaatt aaaaatctcc gcaaataatc ttgatattgc
    31381 taatttcgac gaagatgtgc tgactttttt cgctgagaag ttttccaaaa atgtccgtga
    31441 actcgagggt gcctttaacc gtttgctgtt ctacacaatc aatataaaac caaccgccca
    31501 catcgacatg ggggtcgcta ttgaatcggt ccaatcgcta atcgacgtga aagacaccaa
    31561 aactaaactc agcgagacca aaatcatcaa tgttgtcgcc gactactata acctcacgcc
    31621 gtcacagctg accggcaaga tcagaaccag tcagatcgcg atggccagac atattgccat
    31681 gtatctcatc cgctcgatgc tcgacgtgtc gctgacaaag gtcggaatgg tgttcggagg
    31741 taaggaccat acgactgtta tgaacgccgt cgaaaaagtg gacaaggtgt tgaaaaccga
    31801 caccttctta caaaccgcaa tcgatgattt aaaaaagcgt ttaaagaagt aatgtttata
    31861 aaataaaagg tattcatacc actcgcttgt tgtggtacaa ttactaagaa ggcgtgcgat
    31921 atctaaacag agttatccac acaatccaca gcgctaatta ttattcttta ttcttaagga
    31981 gaagaaaaat gagattaact ataactcgtg aacacctgct taagggctta acgaccgccg
    32041 caaaagcgat tggttcaaaa gtccctgtcc cccttttaat gaatttaaaa ttagtgctca
    32101 acgaaaaggg attgactgtt gtcggcggaa ataacgaatt gacgatcagt acactcgttc
    32161 cgaatatgat tggcgaaaaa gaaataattc gcaacgccaa atacggcgcc acccttgtcg
    32221 ccagcaaaat catcaccgaa atcgccagga ggatggaagg tgaagagttg gcgattgaag
    32281 ttgtcgacga aaccattgtc aaaatcgatg atggcaaatc aagttttaaa ttgaattcaa
    32341 tacgcgccga tgaatatccc gaagttgact tagatccaag cggcgtggtc tttgacatct
    32401 cgtgtcacga cttcacccgc ctgattgaac agaccgcctt tgccgcttca aacaaagagc
    32461 aaagaccgat cctcacggcg gttaatcttg aggcagcggg aggaaaattg atcgcgacag
    32521 cgaccgactc cgcacggctg gcgcgcaaag aagttccgat tgatgatgtc gtttcgtttg
    32581 tctcaaatgt tccggccaga acactgatcg aaatcacgaa aatgtttgaa ggcgccgaaa
    32641 aagtcaccct ggctgtcagc gataaaaaaa tcttgttctc gttcgacaac accctcgtct
    32701 cttctcgcct gatcaacggc gattatccaa acaccaaaaa cataattccc aagactttta
    32761 attacttctt agaagtaaat tctgctgaat taatatctgc catggagagg gtttcgctcc
    32821 tttccgtcga acgcgaaaac gttgtcaaaa tcaccatgac tgaagacgct ttagaggtcg
    32881 ccagcaaatc ggcccaagtt ggttcggcca acgagaaatt atcgaccttc cagttcagcg
    32941 gcgaccgtct ggaaatgagt tttaatagcg actatgttgt gtctgccatc cgcgccgtca
    33001 aagctgagga tgtgacaatc gcgtttatag gcgaaatgaa gccctttgtc atcaagaaca
    33061 acaaagatga ctcggtcgtc cagttagtaa ccccggtgcg cacttactaa gccaaacaga
    33121 cacacaaaag ccacatacga acaataaggg aatttatatt cccttttttg tcgcgctcgt
    33181 gtataatggt ggtgtgagta aaaaagaagg gtttttttat gattaaaaaa acggtggaaa
    33241 ttacgatttc taccgatttt gttactcttg gccagctgct caaaatcgcc gatttaatcg
    33301 gttcgggcgg agaggccaag tcctttcttg cttctcacga aattctcgtt gacggagtca
    33361 gcgacaaccg gcgcggacga aaattgcgcg atgagatggt ggtggagatc gaccaggtcg
    33421 gttaccgaat cgtcaaaaaa tgattatccg caacctcgtg cttcgcaatt tccgaagcta
    33481 cgacttcgcc gaattaagtt ttgacaaagg cctcaacgtc atcgttggcg aaaacgccga
    33541 aggcaagacg aacattgtcg aagccatcca ctacttgtct ctcgcccgtt catttcggac
    33601 cgccgaagac gctaatctca ttcgcgaaaa aagcgatttc gccgcgatcg aggccgaggt
    33661 cagcgaagga tcgacaaaaa agaaaattaa tgttgttctt acgtcctctg gtaaaaaagt
    33721 ttcgtgcaat ggcaaagcca ttaatcgcct gtcagaactc agtgaactcg taaatgttgt
    33781 tgtcttcgag ccgccccgcg tccttctgtt taaggaatcg ccaaaggcgc ggcgggactt
    33841 ccttgacata agtctttcca agcaatcgtc aatttacttg gaatatgtca caaaatacga
    33901 gcgcgcgctg cgcgagcgta atgagctcct aaaactcgac aaaccaaacg aagtccagct
    33961 tgaaatcgtc accgatttgc tgattgaagt cgctgaaaaa gttgtccgct accgcgagat
    34021 gtatgtcgag caaatcaatc aggtgctctc aaaaatcgtc accaccatca aaggagagca
    34081 agaaaacgcg cgaattcgtt atcagccgtt tgcaagacct gatgcgagct tcaaaaagaa
    34141 tgccaaaagc gcgtttagca aagcgctgga aagcgattta cgcaaaaagg tgacttctat
    34201 cggaccccac cgcgaagact ttaaaatgag cctgaacggc aaagatgtcg ccagatacgg
    34261 gtcccagggc gaaaaccgca ttctcgcctt agccctgacg ctgagcccct acttcctgat
    34321 cgaagacaag aacaagcgcc cgatcgtcgt cctcgatgat gtgatgtcag agttagatga
    34381 tgaaaacaaa gaaagattaa tatctttctt aacaaagttc gaacaagtgt ttattacctc
    34441 gacttcgctc gaagtaaaaa acgcgagtgt atacgaagta aaaaaccata agatcgcaag
    34501 gaggaattct taatggaaga aaaagaaaaa gttacaacct tcccggttga tgacgaagac
    34561 gaaaaaggga gtgccgatta cggcgctcgc gatattcagg tactcgaggg tttggccgcc
    34621 gtccggaaaa gaccgggcat gtacatcggc acaacctcct cggcgggact tcaccatctc
    34681 gtctgggaaa tcgtcgacaa ctctatcgac gaagctctcg ccggatattg tgaccgtgtc
    34741 gctttgacaa tcaataaagg cgataccatc accgtctgcg ataacgggcg cggaattccg
    34801 gtcgacatcg tcgagaaaac cggtttgtcg ggtgtcgaga ccgtctttac gatcttacac
    34861 gcaggcggca agttcggcga aggcggtgga tacaaagtat ccggcggtct tcatggcgtc
    34921 ggtggctcgg tcgttaatgc cctctcggaa tggatggata tcaacgtcca taaaggcggc
    34981 aaaaattact tcttgcgctt tgagcgcggc ggaaaaccgg tcggtcactt gcaggtggtc
    35041 ggcgacgctt ccgatcgcgg caccatcgtc acctttaaac ccgatccgac tatttttatc
    35101 gagtccgtcg agttcaacta tgaaatcatc cgcgacagaa tgcggcagat ggcatatttg
    35161 aaccgcggtg tcagaatgat cgtcaccgat gatcgcggcg aaaccccggt taccgaagaa
    35221 ttctattacg aaggcggcat taaagaatat gtcacccaca tcaaccgcaa caaagaacct
    35281 ctttttgagg atgtgattta ttgcgaaggc aaggataatt atgctttccc cgatggcaaa
    35341 atcgcgcata ttcatgccga aattgctatg caatacaaca cttcttataa tccgaccatc
    35401 ttcaccttct gcaataacat aaacacgcac gaaggcggca tgcacgaagc cggctttacg
    35461 cttgccatca accgtgtcgt caatgattat gcgaaagcaa acggctatct taaagatgtt
    35521 gaagaaaact tggcgaccga cgatatcaaa gaaggtttaa ccgccatcgt ctctgtcaag
    35581 caccccaacc ctcagtatga aggtcagacg aaaacgaaat tgggcaactc ggaagttcgc
    35641 aaaatcgtct ccggcatcgt cggcgacggc ttgaatcgct tcctgcttga aaaccccaaa
    35701 ctcgccaaga tcatgatgga taaaatcgtc aatgccgcga tggcgcgcat cgcctccaga
    35761 aaggcccgcg agcaagtccg ccgcaagacg gttctcgaca tcacgacctt gccgggtaaa
    35821 ttagctgact gctcttcgcg aaatcccgaa gagtgcgagc tctacatcgt cgaaggaaac
    35881 tcggctggag gctcggccaa aaacggacgt gaccgcgtta cccaagccat tctaccactt
    35941 cgtggtaaaa tacttaatgt tgaaaaagcg cagccccatc gcgtgtttga aaatgccgaa
    36001 attggtaaca tgattactgc gatcggaggc ggctatagtg ccgaattcga tgtcagcaaa
    36061 atccgctatc acaaaatcgt catcatgacc gatgccgacg ttgatggtag ccatatccgc
    36121 attcttttat taactttctt ctatcgcttt atgagaccgc tcatcgaaca cggctttgtc
    36181 tatatcgccc aaccgccgct ttttaaggtg gaattccgcg gcaagcaata ttattgctat
    36241 aacgacgagc aactcgaagt gctcaagaaa aaactggacc tcaagcccgg atacccgttc
    36301 cagcgctata aaggtctcgg cgagatggat gcccagcagc tctgggaaac aaccatggac
    36361 cccagagccc gcaagatgat gcgcgtctcc ttgactgacg ccatcgaggc cgaagccgtc
    36421 ttcaccgatt tgatgggtga tgccgttctg ccgcgcaaag aattcattca caaaaacgcc
    36481 aagttcgtta aaaacctgga tatttagtaa aggagctcac ccacaatgga agaaaaagaa
    36541 aaaatcgtca cccctgacga agaaaaagaa attgaagaga tcgaatcggg aattgttccc
    36601 ggacttcgcg atgtcgatat ttccaaagaa gttcgcagtt cctttcttga ttattcgatg
    36661 tcggtcatcg tcagccgcgc catccccgat gtccgcgacg gactgaaacc cgttcaccgc
    36721 cgcattatat acggattgaa tgaatcggga atgcaacccg acaaacctta taagaaatgt
    36781 gctcgtatcg tcggtgacgt catgggtaaa tatcacccgc acggcgatgc cgccatctat
    36841 tcgacgctgg ttcgcctggc gcaacctttc tcgatgcgct acaccctcgt tgacggccac
    36901 ggcaactttg gcagcgtcga cggcgacgaa gccgccgcca tgcgttacac cgaagccaga
    36961 atgaccaaac tcgccatgga gatggtgcgc gatatcaacg acgacaccgt tccgtttgtc
    37021 gataactacg acggcaccga aaaagaaccg agcgttttac cttctcgttt ccccaatttg
    37081 ctcgtcaatg gcagcgccgg catcgccgtc ggcatggcca ccaatattcc gcctcataat
    37141 ttaaatgaga cgatcgatgc gacgatcgcc gtcgcccgca atccgctttt gacgccgctt
    37201 gaaatcatgg aaaaatatct tcctggtccc gactttccga ccggaggtac catccttggc
    37261 cgcggtggca ttaaagacgc ttatgaaaca ggcaccggaa gcatcgcttt aagaagcaaa
    37321 gctttgattg aagaaggcga aaatggcaaa aaacgcatca tcatttacga aattccgtat
    37381 caagtcaaca aagcgcggat gatcgagtcg attgccaacc tcgtcaaaga aaagattatc
    37441 gacggcatca ccgatgttcg cgatgaatca aacaaggaag gtattcgcgt cgtcatcgaa
    37501 gttcgccgcg atacgattcc ggaagtaatc ctcaatcagt tatataaaaa cacgcagctg
    37561 caagtcagct atggcatcat catgctctgc cttgtggaag gcgccccgaa ggttttgccg
    37621 atcaatgaaa ttcttctcgg atacatcgac caccaagtcg tcgtcattga aaagcggact
    37681 aaatttcgcc tggccaaaga tgaagcccgc gaccacgtcg tgctcggtct tttgaccgcc
    37741 atcgacaaca ttgacgaaat cgttgaaatg atcaaaaaag ccgcaactcc tgaagaagct
    37801 tcgcaaaaaa tgatgattcg cttcgatctc agcgaaattc agacgcaggc catcctcgcc
    37861 atgacccttc gccggctcac cggccttgaa aagcaaaaac tcgaagctga gagagctcag
    37921 ctggaaatta acatcgctaa gtatcgcgac attctcagct cgcgcgaaaa agtagttgat
    37981 gtcatcgttg aagagctgct cgaaatcaaa gggcgttttg gcgatgagcg caagacggaa
    38041 atcagcaacg aactcgccac aatcgaagac gaaaaactca ttcccgagga agatattgtc
    38101 atcgcgctta ccgttaataa ttatgttaaa cgcatgacaa ccgacacctt cagaacgcaa
    38161 aatcgcggcg gcgtcggtgt caaaggcatt aatactaacg agaacgacat cgtctcaatt
    38221 ctcgtccaga ccaagaccca caccgatgtc ttattcttct caaacctcgg taaagtctac
    38281 cggatgagag gctatcaagt tcccgaaagc ggacgcgtct ctaaaggcac gccgattcaa
    38341 aacttaattt tcaacctcga aaaagaagaa cgcatcatgg cgatcatctc gcttgatgat
    38401 tatcacgaag aacatttctt gttcttcatc acagtcaatg gcatcgtcaa acgcacggca
    38461 ttgtccgaat tcgcctcaat tcgccagaac gggaaaattg ctatttctct taaggagggt
    38521 gacgctcttc tcgatgtcaa acggacggac ggaaacgcct tgattggcct tgcttccagc
    38581 aatggcaaga tggtcaaatt caacgagagc gatgtccgct caatgggccg caccgcggcc
    38641 ggcgtcaaag gtatgagcac ggatggtgct gaagtcgtca gcgccaccac ctcaatcgaa
    38701 ggcgaactcg ttttggctat cacccaaaaa ggttacggaa agatgtcgcc ggccgaaagt
    38761 taccgcctca ccaaacgcgg agcaaaaggc gtcctcacga tcaacacgac tgacaagaac
    38821 ggcaagctcg ttgctttacg cgctgtcaac ggcgacgaag atctgatgat tatcaccgcc
    38881 aacggaacca tcattcggat tcccctcgaa caggtaaagg tggctggacg caacacacaa
    38941 ggcgttcgcg tcatcaagct cgacgggcgc cagaaagtcg tctccatcgc cattgttccg
    39001 catgaagaaa atggtgtcgc cgaagaaaaa agcaacggct ctgaggaaga agaaaacgtc
    39061 gcaattcctg aggaaaacga cgcagatatc taatatttat gaaggttttt gtccactttc
    39121 aacctcgtta tcttaaagac gtttacgaag ggatgcgcct tagaaaaaat atcaaaggcg
    39181 cccttgaatt aacgaatatt gagattgcca aaaacagcat cgactactac gatgtggccc
    39241 attttatttc ggtcgccgat gagatgaaaa tcaacgacgc gatcgagctc ggaaagcccg
    39301 tcgtcttctc ggccttgatg tgcgagtctg atccggcggc caaaataaca gaaattaccc
    39361 gtgatggtga agtgcgccta agcaaccgcg ctctgcgcat tctcaacaaa gtcagcgccg
    39421 tcatcgttcc caacgaagcc ggcgtcaaat ttttgagcga gcaaggcgtt aagacaaaaa
    39481 taatcgtgct ctctccaggt gtcaatattg cccgttttga gaaaagcgat cagttggaag
    39541 ccgacctttt ctatcgctac ttccgcctca gcaagtctgc taaaacagtc gtcgcctttg
    39601 gccagtacga cagccgcgaa gaaatacgca acatcatcga aatcgccaag ctgtgccccg
    39661 agtataaatt tctatatttc ggacaagcca agcgcttatt tttcttgccg agcaagattc
    39721 gccgcctttt aaggcgatct cccaagaacc tgcgttttaa tcccatcatt cccgaggacg
    39781 tctaccggtc cgtgatgatg aacgcaaacg tttttctttt gattaaccgc cgtaaatata
    39841 acgccatcac aatgctcgat gccatcgccg ccaaatgcca aatcatcggc ctcggcccga
    39901 ttgattatga tgctaaggtt ctggcaaaag gctatgctta tattgccaat accttgccgg
    39961 cggtcgccgc cagcatcaac caattcttca tcggtgaact gccgtcaaaa gccgtcaaag
    40021 cttacaatat cgccaaagcc gaaagcttga cggaaatcgg caagcaatta gaaaaaatat
    40081 atacaggact tatcgctatc gataaaggag gccgatgata tgctggatat caaattgatt
    40141 acggagaatc cccaaaaaat tcaggaaggt ttaaaaaaga agttgttcga agtggatttt
    40201 gaacctttac tttctttggt tgaggaacga aaaaaactcc tcattaaagt cgagacttcc
    40261 aaagccgaac aaaatcgcct ctcgggttcc gttccgcaca ttaaaaaaag cggtggcgac
    40321 gtctcggcta tctttgccaa agtcaaagaa ttgtccgcgc aaaatgccgc tgatgaagcg
    40381 catttaaaag tcgtcgaaga gcagattttt gatttcgttg ccgggcttcc caacatcccg
    40441 gacgaagatc ttttacccgg cgggaaagaa aacaatcgcg tcgtctatga atacgggaaa
    40501 atgccaactt ttgattttaa aatcaaagat catgtcgaat tagctgaatc tctcggcctc
    40561 gtcgattatg aaagagcggc gaaaataagc ggttccggaa cgtggattta taccggtcag
    40621 ggcgcaatgc ttgaatgggg gctgatcaat tatttcatcg ccaaccacct tcaagacggc
    40681 taccagttta ttcttccacc ccatatcctc aactacgaat cgggttttgt cgccggacaa
    40741 tttcctaaat ttaatgatgc cgtcttcact cttgaaggcg tgacgcctaa gaagtttatt
    40801 cttcctaccg ccgaaacagc acttgtcaat ttgcatcgca acgagattct cgatgcgaac
    40861 agcttgccgc gcaaatactt cgcctatacg ccctgctatc gctccgaagc cggatcatac
    40921 cgcaccgaag aaagaggcat gattcgcggc tatcagttca acaaagttga aatcgttcaa
    40981 tacacgaccg aggaaggttc cgatgccgcc tttgaagaga tggtcggcaa agccagacgc
    41041 ctcgtcgaac aattgggctt gcattttcac gtcagcaaac ttgccgccgg cgactgttca
    41101 cacgcgatgg cacgcaccta tgatattgaa gtatacattc ccagcatggc gacatataaa
    41161 gaagtatcaa gcgtctccaa cgcccgcgat tatcaagcgc gtcgcggcat gatgcgctat
    41221 cgcgataaag ttactggtaa aacaaagttt cttcacacat taaacgccag cggtctggcc
    41281 acctcacggc tcgtcccggc catcctcgag caatttcagc aagccgatgg aagcgtaatt
    41341 gttcccgaag ttctccgtcc gtttatgggc ggaatcagca aactcgaagc caaaaaataa
    41401 gatatttatt aaattgttgt tataatattt atgccatcgc gatgtattac gatgaaccag
    41461 gtcagggcgg agacgcagca gccttaagtt gctggtacat gtgcggtggt ttatttatat
    41521 gaaaaaaaac gacgaatatt atatgcttct cgccttgaaa gaggccatga aggctcaagc
    41581 gattgacgag gtgccaatcg gcgcgattat cgttaaaaac gataaagtta tttctcgtgg
    41641 ccataaccag cgcgaaaaac gtaatgatcc gaccgcccac gccgaaatta ttgccattcg
    41701 caaagcgtgc aaaaaactcg gcgtctggcg cctcgacggc tgtcagttat atgtcacgat
    41761 tgaaccgtgc tcaatgtgcg cgggaacttt gctctggacg cggattgacc ggattgtttt
    41821 tggggcaaaa gatccgaagg gcggagctct cggaagttcc taccatcttt ttgagcagaa
    41881 aaacctgaat catcatcccg tcatttgtgg aggcgtctta gaaaacgagt gcggtcaatt
    41941 ggtttctgct ttttttcaaa acaaacggct gctgcaaagc aaataattat aagaaatata
    42001 caaaacatag tagaattcac ttatacttgt tagtagaaaa gtgatacttt tcacctatgg
    42061 atatggaaaa gaaaactcta aactttattc ttgtcaccct tgcttttttt cttgctggct
    42121 gcgccggaac ccccgactcc accagcgata gcgagagtgg cggtaacttt actttttgcg
    42181 aagatgattc cgcctttttt gactttagtg aagacgacac tttgaatgcc gctattgaag
    42241 aattgcccct cggcttacaa accgatgaag tacgcgatga catcttagaa atattaggca
    42301 gcaacgacgg atacgccaat cacgtcagct atagtttaga aatcatgacc gattacgacg
    42361 ttctcagtac tctcgatgac gatcgcgatt tcttcggaga atacgccaaa gaagtctcgc
    42421 gttacgatgc cgaaaaagtt ttgtctggca cctatgatgt cgacgaaaac tattacgacg
    42481 aggccgaaag tgaagaagtt acggtcgatt taaccgccga ctatcaagtg tttcgcaatg
    42541 gtttctgccc ctcgatttct cgctattacg aaatttataa tttcgccggc gaagataacg
    42601 atgttgctct cgaaaaagtt tatggcacgg aaaacttcat tcgcaaacta tatttaattg
    42661 acgggcagga cgaagccctt aaactcaata atcacaatga cgattatgag gaagacgaaa
    42721 acgtcgaaga tttgactttt acggccacca aatttttaag caaagccgat ggtgaaattg
    42781 atgccgatcc aggtttaaat actagcacca gtttagaaat cagcgtcata gcttcttatg
    42841 atccggccgg cgcagccctc ggaggtaagg agattcactc aattttaatc gtcgatggga
    42901 tgattaaaaa agtccgcgac ttcagcggga cgtttgaagt tgtcggaatc gatgaaatct
    42961 atcgcacgag cgaagaaaaa atcgctacct tcagcgtcca agataacggt gcttttaccg
    43021 gcacattagt aaatcctgac gattttacct tttccaatac cgcacccaag ctataaaaaa
    43081 tttctaaatt aaaacttgtc atttgtcagt ttaatttatt aaactaataa tagtcttgtg
    43141 agtattggct cacgcccttc ttttttccag ttgtgcgcgc tggaggaaaa atccgttttc
    43201 agccgtttta aaaagaattg taatcattta tcccgctatt tgattagtta gcgacgacag
    43261 gtgttacaac cgaccagcga aaggtggtgg ctcatatgtt tattcgcccc gatgtcgaaa
    43321 gggccataaa actcaaccgt cccgtcgtcg ccttggagtc gacgattatt tcgcacggaa
    43381 tgccatatcc tgaaaatgtc aaaacagctc tcatgctcga aaatatcatt tctgaggccg
    43441 gcgccgtgcc ggccacaatc ggcatcatcg acggcgacat tgtcgtcggg atgagccccg
    43501 aacaaattga agaattcgga aagcggagag atattcgcaa agtgtcgcgt cgtgatttgc
    43561 cggttgtcgt cgccaagaaa gaatggggcg cgacgacggt ggcggcgacg atgatcattg
    43621 ccgaaaaagc tggtattgaa gtgtttgtca ccggtggaat tgggggtgtg cacagaaacg
    43681 ccgcgacaac atttgacatc tcggctgatt tagaggagtt tgcgatgacc gatgtcaccg
    43741 ttatctcttc tggagcaaaa gccattttag acttgccgct gacgcttgaa taccttgaga
    43801 ctaaaggcgt gactgttttg tcttatcaaa gcgacgaatt tgccagcttc tattcgcgtg
    43861 attcaggctt aaaaatcgaa gatcgaatcg aaagcgccga cgaagccgcc gccatcatca
    43921 aaaacaaacg cgatttaaag ttaagaggcg gtattttgat ttccaatccg gttccgcgcg
    43981 aagcagaaat gcccaaagcc gaaattgatc aagccattga cgaggctctg ggcaaagctg
    44041 aaaaaaatgg cattaaggga aaagaagtta cgccatttct gctgcgagaa atcgcgacta
    44101 ttaccaaagg taaatcttta gcagcaaaca ttcgactaat caaaaataac gcattattgg
    44161 gggccgcagt cgcaaaagcg ctcgcgcaac ttaataccaa ggggaacaaa catgaaaaaa
    44221 cttaattcgt tcagaggggc tctcttcagt ctcgcgcagg aaaaaccgct cgccaaaatc
    44281 agcattgaag aattgtgcga tcgcgtcggc gtcaaacgcc aaactttcta ttatcacttt
    44341 aaagacatgg gtgattttat cgatgccatc ttggttgata tctgcgaaaa acgcagtgat
    44401 aaggaaggcg acctgaatca agacgcacgc tttatcgccg atctttacgc gaataacagc
    44461 gagatcttaa aaaactttct gacgtcgcct tacgcaatta atataattaa tttcgttcac
    44521 aactatttgc acgtgagatc ttataaccgc ctcgccgcga atgctaaata taaaagtttg
    44581 cctggcgaac agataaaaag tgtttcgcgc atcgtcgcca gcattattgt ttcggaactt
    44641 tcctattgga ttaataccgg aatggaagaa gataagaatc atttgctggc acgctttgat
    44701 gccattttga aaaacgtcga gaagatgatg gttgaaaatg ccttgcgcgg cacgatcgaa
    44761 aaaaggaaac acccagaact tgccgaagac gaaaaaaata agcactaaac aaggagaaag
    44821 acatgattga ttttgatttt gtggcaccga caaaattatt ttttggacgg ggcaaagaaa
    44881 aagatgtagg agccataatt aaatcttatg gctttaagaa agttttattt cactatggta
    44941 caggatcgat taaatcatca ggtctttatc agacaattgt ttcttctttg aagaacaatg
    45001 atgtcgattt tattgaattt ggtggcgtcc gcgtcaatcc cacccactcg atggtgcgcg
    45061 ctggcgtcta tttagccaga aaagaagaca tcgattttat cctcgctgtc ggcggcggat
    45121 cggtcatcga ttccgcgaaa gcaattgcgg ccggtttctt ttacgatggc gatccctttg
    45181 attttaacct tcatctcgcc aagcccaaac aagctctgag cgtcggcgtc gtcctgacaa
    45241 tcgcggccgc tggatcggag ctttcggctt cctgtgttat ctctaacgac gagacaggtt
    45301 taaaacgtgg ctttaacagc gacttaaatc gccccctctt cgccatctgc aatcccgaac
    45361 tgacttattc ggtcagcaaa tatcaaaccg gatgcggcat agtcgacatc atctcgcatt
    45421 cttttgagcg gtatttctca ccgagcgaaa aaaaagaatt agctgacgaa ttcgctttag
    45481 gcgtcattcg ccacgtcgtt gaagtcggga aagcagcgat tgataatccc ggtgattacg
    45541 atgcgcgagc atctttgatg ctagccagtt ccttttccca caatggcatc accggactcg
    45601 gcaagaaagt caacatgccc attcatgcga ttgcccacgg cctgagcggc cacgatccgc
    45661 gcattgccca tggcgcagcc ttgtcggtca tgattccggc ctggatggct tttgtcaaag
    45721 atgccgagat cgataagttt tctgcctttg cccgcgaagt gtttaaaatc cattttgtaa
    45781 gcagagaaga aagtgctaat attggtgtac gcgaactccg tcgtttcttt gaagaaatcg
    45841 gcatgccggt gacgcttcgc gaattaggaa ttgataaaca agacatcccg cgtctagtcg
    45901 cgatgatcac ggaaaacggt acacgtacgg ttggccattc cgtccgaccg ctagatgcta
    45961 aggacattga agcgcttttg ctatcgtgtc tctagggagg ttttattttt atgaaaaaag
    46021 aagatgccgc gagtttaatt gtctatgccg tgatgattgg cctggccctt tttgtcgggc
    46081 tgggaattat tcagccggtc tttgccgcca atgcttcggt gggcggaagt caatacgcct
    46141 ttgccattat ctctgttatt atcggcgtct ttatcaacgc tatcttactc gaagttggcc
    46201 acatcttagg cggcctcatc gggaaatatc gcattcactc cgtcaatatc tttggctttt
    46261 gctttcacaa aaagagtggc aaaacgcact ttgcttttag aatgttcgac ggcttgaccg
    46321 gcgagaccaa gtttttgcct aaaagcgaga aatcaaatcc gcggccggcg actcttttgc
    46381 cggttcttct ctaccttatt gaactcgttg ccgcgattat actctacgcc ctcatttcga
    46441 caaatgtcga tgtcgatctc gtctggctct caatcggagc gattatcgtt gcctccattg
    46501 gtggcatgat gactctttat aacatcgtcc cctttaaact tgatgccatc accgacggtt
    46561 atcgcctcac gcttttcgca aagaaaataa acatcgtcgc ctataacgaa ttgatgctcg
    46621 cggaaaacgg cgatgagcca tttacgcccc gcatctttga tgaaatcacc gattttaccg
    46681 cagaagtcaa tctcatctcc gtataccgca atattaaaga gaaaaaattt gcggaggccg
    46741 agacgatact gactaatatc atcgccaatg aagcaaagac tagcaactcg acgcataacc
    46801 gcgccatcgc gcaacttctc ttcttgaaaa taatgaacga acctctggaa gacgcgaaag
    46861 cctactatgc gacaattccc acctcgattc accgttttat cgccaacgat ttatcgatgc
    46921 catcgcttcg cgcttatgtg ctcatcgccg gattgatgga tgacgcgcaa ggcgagatca
    46981 tgtattgcct ggaccgcaaa aagaaagcga tcaaatacac cgcccccggc attgccgaaa
    47041 ttgaaaactc cttatttaag gaggcccttg ataaagtcaa ggtcgctcac cctgattgga
    47101 agttggaaca gtaacacaaa aagacctcct cggaggtctt tgttttagat tgatgatgac
    47161 tgagttattt gtcttttttt agaagatcgc ggatttcggt cagcaagacg atgtcggcag
    47221 ccggtgccgg cgtgggtgcg ggttctggtt ctggctcgcc ttcttttttg tgtagtttgg
    47281 ccaaagcggc ttctttagct ttttgaatgc tggcgaagat tttgatgaca gcaaagagaa
    47341 ttaaggcgat taagaggaag ttgatgattg cagttaatac taagccccaa tcaagtttgc
    47401 tgagcaccga gtagtaaatg ccgttattgg caagaacggc tgccccggcc agtatttcag
    47461 cggtcgtcgc cttaatgctg ttaggcaaga cggtgtagaa attgttgaga ccgccattga
    47521 ctaacaagcc gattaggggc ataaagacat gggcgacgag cgcggtgatg atcgccgaaa
    47581 aagctccgcc gataatgaca ccgaccgcga gttgcatgac attgccgcgg ctgataaagt
    47641 ctttgaattc tttaaaaatt ttcattttcg atttctccta accactatct taaaagataa
    47701 ataaaatatt acaagtgtaa aacaagcatt ttctttctct ttgacaaaat gagcaaagat
    47761 tgggataatg tgggcaattc taaggagaaa acacatgaat aaagtagcca tcttcgtcga
    47821 ctcgacagcc gatctaggca aagaacaata caataaactt gatatacacg tcgtccctct
    47881 cctcgtcaac atcggcggaa acgcttataa agatggagag gaaattgatt caaccaaact
    47941 ttatcaatta gtcgccgaac acggcgagct cccgacgacg gccgctgtcc ctctttatac
    48001 ttttgttgaa gctttcaaac catttatcga taagggttat gatatttttt acgtcggtat
    48061 cggcgggaaa ttatcggcga cccttcagtc cgtcgtcttg gcggcccagg aatttccgga
    48121 gggacgggtt ttctattcgg attcgaataa tctctcaagc ggttcgggta tcctcgcctt
    48181 aaaagctgtt aaataccgcg atgaaggtaa gtcggcagcc gacatcaaag cattgatcga
    48241 tgctcacgcc cccaaagtaa attctctttt cgctatcgag accctcgatt atcttcacaa
    48301 agggggtcgc tgcagcggaa ccgccaaagt attaggaaaa ttgtttcaca tccatcccat
    48361 catcaaagtt actgacggta agctgatcgt ctataagaaa ccgcggggac ggatgagttc
    48421 ggctctcgat gaattagcgg gtgaactaaa gagtcacctt cctcatgttg attctgatca
    48481 catcatgatt acccatgccg gagtagaaca ggccaacctc gattatttag tcaaaaaagt
    48541 tggcgcactc gtcaacccga atagtgttat gatcaccaac gccggcgctg tcatctcctc
    48601 tcactgcgga tacggaacaa tcggcgttct ctaccttaaa aagtagaaaa atagtcgcgt
    48661 tttgcactaa aaaacgtcgg ttttgccgac gttttaattt tacatttttc tagaaccagg
    48721 tctcgatggg cgaccaatcg taaccgtttt tgccgaactc cggaccccat cctcccgcat
    48781 agtttttgta ctcgtaaaaa gtgtcatagt gattgtctgt ataaaaaaca acaggaatat
    48841 tatttccgta ttcgtagatg ccgccgagag taaagacaac gcgcagggca ccgcgattcc
    48901 aactagatga ttcggcatat ccgtctccgc cgatatcggc ctcgttatag acatcgtcta
    48961 atgagtaagg caaaaatgaa tagttgcttt catatgggcc gggataaatg cggcattcgt
    49021 ttccgtataa tccgtagcag acatcttttg attcgccata tccatttctt tcggtcgtga
    49081 aatagtagtt atccggaaca tcattgaaag ccacgatata agccgcgaca tcttcgacat
    49141 ccgtgtagta ttcatcctta tagatttctt ttaaaacctg accgtattca tcataaacaa
    49201 aaccgatgtt tcctgtaccg gggacaggat taagacgata ctctccgacg ggcacttcgc
    49261 cagttgactc gctaggactc gctccagtcg aagtatcggt ggcggtatct gatgaaatat
    49321 gttcgcttga gattgatgta tcagaagaag aggtgtcgac atctgaaggg gaaacgcttg
    49381 aatccccaaa agaggaacaa gacgaaagaa taagcgtcga caagattaaa aatgacagtg
    49441 agtggcgcat tgctttgaac ttatcgttac tttccggaaa tcgctaaatt tttgatcaaa
    49501 aggctcggcg cactgatgcc ggacaattgc agttcggcat tgttggcgac ctcgcgaaca
    49561 ttcatgaata aatcgagcaa gttgccggcg acagtgataa ggctgagcgg ttctgccaac
    49621 ttgccatcgc gaatcataaa gccttcggct tgcaatgaga aattacctga ttgcggattt
    49681 aacccggcat gaagtccggc gacgctggaa atatagacac cagtctttat ctttgccaat
    49741 aaatcggctt ctgatttacg acctggcttg attgaaggat tcacgaaacc gacgccgatt
    49801 tttgaaccgc cgcgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcat
    49921 cgtcaaagta gcggaagaag caattcttta aaagaggttg ttcgagaatt gtgactttct
    49981 tgctggccac ttgctgattg agtttgccaa tgaagagcga agaatgcttt tgtacttctt
    50041 cagcgcttgc gctttccaga tacggatcaa ggaaacttgc agccgtcttc ggattgagga
    50101 tgacggggta ctttttgctg tcgcattgtg taccgcctaa tttgctggtc gcgttttcga
    50161 cgacctggtc gacgaatttg tcaatatcaa gctcttttgg atcatttgat aagaggactt
    50221 tgtagccggt ttttacttct tcgccttgtt tgacgacgac tgaggcggca taaacatagt
    50281 agtttgattt gctcttcaga ttaaggccat acgagttgac caaattaacc tcgattgcgt
    50341 cttcggaata ctgcacaccc tcaatttcga cgatgcgcgg atcagccttt ttgagcttgg
    50401 cctcaatctc aaagagattt ttcatctttt gatcgataga aatggccgga atatctttgt
    50461 tgtataaatt gcgcttttga tatttgtcgc tgcccttgaa taaaagagct ttatcagcct
    50521 tttcgattac cttggcgttg ctgatgattt cgtcgacgag atgctcgggt gtcttggcat
    50581 cgactttttc ggttcttacg actccgaact tatcgttata gattccgcgg gctgacattg
    50641 aaaatgtttc gctggtggaa taagacgata cttcggaatg aaatagttca aaggaaagcg
    50701 aatacttttt cgcggagtat agctcgctgg cttcaatccc ttttgatttt gctaactcaa
    50761 agaatttttt atagttcatt ttaaaactcc tcctctgccc ccgacgagaa tctcgcttac
    50821 gcggatcgtc ggttgcccga catcggcggg aatgctgcca ctgaccgagc cgcacatgcc
    50881 ctgggctctc tgcaggttgt tgccgaccat gtcgattttc atcaggatat cggctcccga
    50941 accaatcaga gtcgcgccgc gaacgggctc agcaattttg ccatcgcgaa tgatgtaggc
    51001 ttcgctgacc gagaagttaa attcaccggt ggcggggttg acgcttccgc cccccattga
    51061 agcggcatag aggccgagct tggtggcggc gattatttct tcgtgtgtgg aatttccggc
    51121 ggcgataaag gtattggaca tccgtgacgt cggctcgtat ttatacgatt cacggcggca
    51181 ggcaccgtta gctttggcgt tcatccgccg gccattgaat gagtcgacga ggaaagaagt
    51241 gcagattccg tctttaatca gaacattgcg ctgagtggga tttccttcat cgtcgatatt
    51301 gtttgaaccc catccgttgg cgatggtgcc atcatcgatg gcagtcacta gcggcgaagc
    51361 gatttgttta cctaaactgt cggagaaaac cgatagtttt ttggcgactg cggtggcctc
    51421 cagagagtgt ccgcaggctt cgtgaaagac gacgccgccg aaaccattgc cgatgacgac
    51481 cggcatcttg ccgctggggc attcggccgc ggtcagcatc ttaatcgcta attcggccac
    51541 tctttttgcc gtatcgcgaa cgctgatttt ggcagtgaaa aattccatgc cggccgttgc
    51601 gcccggacct tcaaaactag tttctatttt gccgttttcg ctggcgatgg ccacgaaagc
    51661 catgcggccg cgctctttaa aatcagcgaa ttgtttgccg tctgaattaa agatggtgac
    51721 gactttgcgg ttattcataa atgagcattg aatgcggacg atgcgcttat cataagatga
    51781 gatgacctca tgggcttctt tcatcagagc gattttttct tcgcgcgaga cttcgctaag
    51841 cggcctttca tagacatggg cgtttttgac gcgcacttta ttaatctttt cgacggtgat
    51901 cagccgttcg ccttgaaatg atttagcaag ggaagtcgct aaattcatta gtcctttgcg
    51961 ggaaatgtcg ttcgtgtatc cataaacgct ttgcagttta tttaaaagac gaatgccggc
    52021 gccgtaagtc gtgctgctga gcgacgattc ggtcttgccg ttttccaggc ttaaaaagga
    52081 cgagtcgttt tgttctaagt atatttcagc gaaatccgca ccggtgacaa gaccggcgtt
    52141 taaaacatca atcgctaaag attttccaat catactagat cctcctttat tttggtggga
    52201 acaattttag cattattcgt ttgaataata caccctaata cttgtaaaaa tgtataataa
    52261 ctttatgttc gatgccgaat tgctggaaaa tttagaaaag cactttcagg aaaagaaggt
    52321 cctttttaaa aaagaagatt ttgcctatat cgccgggaaa gacacgttta agggcaagat
    52381 gctcggcttc ttaacaactg attattatcg gcgcgaaaaa cttattcgcg ggggcagtct
    52441 cgtctatggt tatgtctttc ggacatggac gaatgaagtg actttcacca gaccctatcc
    52501 gatgtggatt cttttttcgc cttcccaggc cttcaaaaac gatcccgatc tatttgtttc
    52561 aatcctgtcc gctcttcagg caattgaact tcccaagcgc ggtcaaagcg aactccgtaa
    52621 actatttacg atgctgaatg ccgagctgag cgaaccgaaa tatttcttaa ttcccgagcc
    52681 ttatgctcaa ggtcacctcg tctatctttc gatggcctat caccggccgt gtcacaataa
    52741 caatttaaag ttgggaatta atccctttat tatgggagcc tcaatttcta aagaaatttt
    52801 atatttgcca accaaatact gggatgaatc attcaaaaaa gcctattacg aattttagtc
    52861 gatttggcaa ggaatctgtt tgctttatga aataagtaat ggtaatatta catggcaact
    52921 ttctttggaa ccaccaaggt ccaaggcgga aggagaaact tatgaagaaa gacattcacc
    52981 ctaagtacaa tcgcgtcact gtcacctgtg tcacctgcgg atcggtattc gagaccggct
    53041 caaccatgaa ggaaattcgt gttgatacct gctcaaactg ccactcctac tacaccggca
    53101 agcagagatt cactcaggcc gatggccgcg tcgatacttt caataagaaa tacggcttaa
    53161 aaaacaagta acacaagaaa gaccgccgta tcggcggttt ttactaatca tgaaaactaa
    53221 gttttacatc acaaccccca tctactatcc cagcgcgaag gcccacatcg gccacgccta
    53281 tacgaccgtt gtcgccgatg ttttagcgcg ctacaaaaga atgcgcgggt ttgcgactta
    53341 ttacctgacc ggcaccgacg aacacggcga aaaaattcag aataatgcca agctggccgg
    53401 caaaagcgag cagcaattcg tcgatgacat tgccaacgac tttaagaagc tttggaagac
    53461 gttagaaatc agcaatgatg atttcatccg cacgaccgaa gaacgacaca ccaaggtcgt
    53521 gaagagcatt tttacaaaac tttataatca aggcgatatt tatctcggca aatacgaggg
    53581 ttggtattgc acaccgtgcg agtctttttg gacggaaacg caagtcggac ctgaaaggct
    53641 ttgtcccgac tgtcatcgcc cggttcacac cgccagcgaa gacgcttact ttttcaagac
    53701 gcgaaagtat gttgatcaac ttttaaaaat catggacgat aatccgaagt ttttactgcc
    53761 ggtaaatcgt aaaaacgaga tgatcaatac gtttatcaaa cctggtcttg aagacttatg
    53821 tgtatcacgc acatccttca gctggggcat tccgacaaac gaagatcccc gccatgtcgt
    53881 ctacgtttgg ctcgatgcct taagcaatta tattacggcc cttggctacg attcggataa
    53941 tccggaattg tttaataaat tttgggctga tgaaaacagc gaaatcgtcc acctcatggg
    54001 cgcggacatc tcgcgttttc acgcgattta ctggccgatg tttttgatgg gtctcggcgt
    54061 ccgtgttccc gaccgtctct ttgttcatgg tttactgatg atgaaagacg gaaagatgtc
    54121 caaatccaaa ggtaatgtcg tctcccctta tcctttaatc gaacgttacg gcgtcgatgc
    54181 cgtccgctat tatctgacca gagaagtcgt ctttggaagc gacggacaat ttacgcctga
    54241 gcagtttgtc gagcgcatca ataacgacct cgccaacgac tttggaaacc tcctcaatcg
    54301 cactttggcg atgattaata agtactttga aggtgtcgtt ccgacttatg atggtgatgt
    54361 cacgccgttt gacaaggatt tgcgcactct tgcggaagaa accgttaaag gatacgaaga
    54421 agatctcgat gaccttaaaa ttacggatgc ctttagcaaa gtaaataacc ttgtggc
//
LOCUS       DAOSSL010000004        36557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1275_length_36557_cov_16.743137, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000004 DAOSSL010000000
VERSION     DAOSSL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 36557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36557
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_36557_cov_16.743137"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..901
                     /gene="rpsD"
                     /locus_tag="PLH17_00965"
     CDS             296..901
                     /gene="rpsD"
                     /locus_tag="PLH17_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOR20384.1"
                     /translation="MRFTGSDYKRSRRLGFSTLETGEELRKRSYGPGQHGNARKRKPS
                     EYGKQLVEKQKLRHMYGVNERQFRRLFAIATKSSEVTGIAFFRILESRLDNLVFRMGF
                     SRTRRGAKQLVNHGHVTVNGKKVDISSYLCSVNDIIAIRESSKNLKVVKEALESKPSA
                     VGYVKVDAVKLEGTFLRAPERNELPQEIVEAQIIEYYNRLL"
     gene            complement(963..2216)
                     /gene="tyrS"
                     /locus_tag="PLH17_00970"
     CDS             complement(963..2216)
                     /gene="tyrS"
                     /locus_tag="PLH17_00970"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOR20385.1"
                     /translation="MVNIIDELNYRGLIKDYSDEKEIRELLSTPQTIYCGFDPSAASM
                     HIGNFVMISLLMRLQRAGHRVIPVVGGATGMIGDPSGKSKERNLLEGNELKKNTEAIR
                     AQLARFINLEDSKRGLIVNNYDWLSKISMLDYLRDYGKYFPINYLLNKEIISSRLENG
                     ISYTEFSYNLLQAIDFLTLYRNHGCRIQVGGSDQWGNLTSGLELIRKVEGGDAKVGVM
                     TSHLIVDSTGRKFGKSEGGALYLDSEITSPYKLYQYFINVSDADAIRYLKVFTFLSRE
                     EIETAAKAHEANPGLRQAQKTLAFEVVKIIHGQARAEEAIKMSESLFSGDFVTMSEKN
                     LIDVLGDLEVKVDSGIILEDVLIAAKAASSKREAREFLKGGSIALNGQKAIDGTIQLT
                     GADALHGKYYVLRRGKKNYYLINVR"
     gene            complement(2220..3221)
                     /locus_tag="PLH17_00975"
     CDS             complement(2220..3221)
                     /locus_tag="PLH17_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR20386.1"
                     /translation="MDYLKDRVTIYEVAKVSGVSLATVSRVINKQGNVTEATRLKVEA
                     TIQKLGYKPSGLARALATNVTTNIGIVIPSANYVYISNMLNGITEVAKEKGFVLTLFV
                     TSYSRSDAIAMVEKVITSHVDGVIIFDDQLDKEDIEKINSYSVPTIVINNKITGNRIG
                     CINFGYEHAVKRMLKARYEKNDMKPAVFLHVHNAGRLLARIEKAFIDFHNEIGVPYRI
                     FNVDDSSERTYLDFMEYFKKHRKGFFIAYRDSIGAAIVNAATDSGLKVPDDVEVISII
                     GTKYAEIIRPTLSSFYINMQEVGKRAMYMLIDLSNGELNEKAYKFESTYTARNSTKE"
     gene            complement(3302..4402)
                     /locus_tag="PLH17_00980"
     CDS             complement(3302..4402)
                     /locus_tag="PLH17_00980"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOR20387.1"
                     /translation="MELEIVLKKQGKISRLTNRTFAFDERLRSGFYAANYFLKARDIV
                     KEHNPHHIVTMQFFQRTEEAMVCGLDEVIALVHTFAVEPENLVIEALNDGDIIAANEP
                     VLKITGKYENFGFLESMIDGILARRSSVATNVHRVVAKLNGTPCFSMADRQDDYLTQV
                     GDGYATYVAGIDKVSTDAQGYWWGGRGMGTMPHALIQVCGGDIIQAATLYQKTFPHEK
                     VTALIDYNNNVVHDSLVLARHLKNDLKAVRVDTSKALIDHYFDGKDTSGFNAHGVCKE
                     LIFALRKALDSEGFNFVKIIVSSSFDVTKITEWVKLGVPVDMYGVGTAFVNNTTVGFT
                     GDLVRLDGRPEAKEGRRDIPSRRLQKVPYPIY"
     gene            complement(4406..5008)
                     /locus_tag="PLH17_00985"
     CDS             complement(4406..5008)
                     /locus_tag="PLH17_00985"
                     /inference="COORDINATES: protein motif:HMM:NF026144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HOR20388.1"
                     /translation="MYRLFYNYHTLGDVLMIVFNNQKRATHLDKRNDVTLIYSDYELI
                     GINLFNISKIVRIKAQGMIVSPAKEFLEVINHILKNEGLEPLSEQSESGFVAGQILEL
                     IECEDHPFKIAKVDIGLRVIYTKSENEEIAPYSMVVIALADTLLFNGETVVVETHDGM
                     YFEGRICTYKDLNLESPKGDEKTYIIEEFMEIGQDFFAKE"
     gene            complement(5010..6098)
                     /locus_tag="PLH17_00990"
     CDS             complement(5010..6098)
                     /locus_tag="PLH17_00990"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR20389.1"
                     /translation="MKLTNRQLSLLESVTQFISVSGDENEVRNFLKQEFLDKGLELVS
                     DNLGSLYGLKKSKIINAPKVMVSAHMDEIGFMLLYIQDNGLIKTSALGGHSKETLSAH
                     RVLLKTKSGKKYYGAIASIPPHLKSEKDQDEDRYIEELLFDFGFTSKEEALAAGLSIG
                     DSIVVLGDFKILNNGQRLLAKAFDNRYGIALALDVLDELIGEDLPYDLYIGANVQEEV
                     GLRGAQTAAHLINPDFAVVLDCSPARDIGAKKDEYGRLGEGVLIRYLDRAMIAFPELL
                     DMQIQACRETGVKYQYFDSPGGTDAGSIHKANSGTLTLTHCICARGIHSPSSIIDVDD
                     YLAARKSLLHILRHFDASRLANLKGSRR"
     gene            complement(6101..6721)
                     /gene="trmB"
                     /locus_tag="PLH17_00995"
     CDS             complement(6101..6721)
                     /gene="trmB"
                     /locus_tag="PLH17_00995"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOR20390.1"
                     /translation="MRTRYKPWAKPYLDDHQEIVIKSLDDDPSFFADGPLEMEIGCGK
                     GDFIVALATNNPNTKYLAIEVSAMVAAMAARKAVENKLQNIRFIVDDVAKILPLFLDE
                     TLGAIYLNFSDPWPKKKHEKRRLTFPAKLLEYTRILKKGGYLFFKSDNDALYDYSLAT
                     IKESPLELIAYTDNYSALAEGDAMSEYEKQFRNLSKNINRIVARRK"
     gene            complement(6718..8082)
                     /locus_tag="PLH17_01000"
     CDS             complement(6718..8082)
                     /locus_tag="PLH17_01000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HOR20391.1"
                     /translation="MKQDLDFIELTNPYRANAVRALQELIKINSVFDEKSASSTAPYG
                     AGVEKALKYVGRLARQFGFDVDYCDNRATEMTFGQGERLISIFAHADVVPVGSGWKHE
                     PFGAQIEKGKVFGRGVSDDKGPLIAAFYAIKALKDNNLINNYRVRLVVGGDEERGSSC
                     LKYYFKDLKKEHPTYGFTPDANFPVIYGEKGISNFRSRINLVLPGIMKINGGEAVNSV
                     IDSTLVVMKSDAKFETFLKGLKNKTTIENINGVSKITFHGKSAHGSTPQAGVNAALFA
                     IEALGTFYQNDILLKIFAQFRHFDGKPFKGYAKSKDLGETTYNLGVIAYDGKALELKV
                     NFRYPEDVDDADFVRRFDEASGLTSEIYSRSPSLLFPLKSPLIKTLLNAYREETGDKR
                     SKPLTMGGGTYAKEAPSTVAFGAAFPDDNPHMHEADEHLILNNFYISMAIYARAVHML
                     GNLK"
     gene            complement(8066..8707)
                     /locus_tag="PLH17_01005"
     CDS             complement(8066..8707)
                     /locus_tag="PLH17_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOR20392.1"
                     /translation="MRNKIIRTIVFDAMFIAIIAIMAFVPNIGLITVSPLISFTLLHI
                     PVLIGAALFGWKRGLIYGIAFGLLTLIQAATQPRGAFDPLFINPLISVLPRALFGLVA
                     GFSLELLRKMPRLYQKGLFIAATSLVMTLFHTFLVITSIWLVYKADVLRILQIQDAQS
                     FHWINFIGLLAIVFGALIEAGIAAVITPAIAVPIYHKYPFLLNKKEQNNETRP"
     gene            complement(8697..9476)
                     /locus_tag="PLH17_01010"
     CDS             complement(8697..9476)
                     /locus_tag="PLH17_01010"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOR20393.1"
                     /translation="MLLTVDVGNTLIAFGVFKQSRLVASFKTATDIKKSLDEYVSTIE
                     TFIQFRKIDKSAFRGAIISSVVPFLTEVMREALRLSFKLESIVLTPGLKTGLPILIDH
                     PNELGSDLVADAVGAIAKYGSPLIIVDLGTAIKLLAIDHKGAFVGATFYPGLYVAVDA
                     LIGRAAQLSRVSLSRPKKVIGKNTKDSTNAGAIYGTASMIDGLVKKFEEELGYPTRHI
                     LTGGDAIYVKDLLADFIYDEILLLDGLRVIYERNEARKNEK"
     gene            complement(9561..11963)
                     /gene="leuS"
                     /locus_tag="PLH17_01015"
     CDS             complement(9561..11963)
                     /gene="leuS"
                     /locus_tag="PLH17_01015"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOR20394.1"
                     /translation="MKFNHLEIEKKWAQKWKDGNVFKTDTSDFSKPKYYILDMFPYPS
                     ADGLHVGHPKGYTASDTIARMKRMQGFNVLHPIGWDAFGLPAEQFAIKKNRHPEDFTI
                     ANINNFRRQIQAIGLSYDFSREVNTTDPSYYRWTQWIFLKLFEKDLAYVDSIPVNYCP
                     ELGTVLANEEVINGKSERGGYDVIRLPMRQWLLRITKYADRLLSGLSQVDWPKSTIDM
                     QTNWIGRSEGAKITFKVKGQNTSFDVFTTRADTLYGCTYCVLAPEHELVKKIANPDKL
                     PAIEAYLSQIAAKSDLERTELNKEKSGVFIGAYAINPINEKEVPIYIADYVLATYGTG
                     AVMAVPAHDQRDYEFAHKHKLPIVQVLEGDISKRAMEEDAKHINSAFADGLNNQEATK
                     VIVAHLNKKGLGQFQISYRLRDWLFSRQRYWGEPIPVALMDDGKIEPLQEGELPLILP
                     KLSEYKPSGTGESPLVHAGEWLEVTIDGRKGKRETNTMPQWAGSCWYYLRYIDPHNDQ
                     AIGDSELLAHWLPVDLYIGGAEHAVLHLLYARFWHKFLYDLGIVKCEEPFKKLFHQGM
                     ILGENNEKMSKSRGNVVNPDDVIAKYGADTLRLYEMFMGPLEASLPWSENGLDGARRF
                     LERAFRLFSEDEFLAKHSDTNDGSLDYVYNFTVKKVSEDYQNLQFNTAIAQLMIFVNE
                     AYKADKLYRPYLEGFVKMLSCVAPFIGEEIWESLGHQEGITYAPWPTFDESKLVRSQV
                     TMAVSVNGKLRGTFEADVEAEEEELRKRALTLEGVQRNIEGKEIVKIIIVKGRIVNIV
                     VR"
     gene            complement(11968..13401)
                     /locus_tag="PLH17_01020"
     CDS             complement(11968..13401)
                     /locus_tag="PLH17_01020"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOR20395.1"
                     /translation="MKIAMVTSEANPFVKSGGLADVTYSLSRELVMLGEEVALFMPLY
                     QSIIAQFGDKLQHVAEFDVPMSWRRQYAKVYRTYVDGITFYFIQNDYYFGRDNLYGYN
                     DDLERFAFFTIAVRNAFKALGFKADIVHLHDWQPGMLPVIIREQNQNDPFFQNFKFVL
                     TIHNPAFKGMFDKYFLNNFYELSDSLYDSGKVRFENVVSTLKAAIVYSDKIATVSYTH
                     RDELLSFDSEHGLSSVLELRRQDFCGILNGIDYAEFNPKADRAIAYLFNGVNVTKGKD
                     ENKAALLKQFNLPNRGQPVFGLVSRLTWQKGIRLIIAKARHIVANGGSIIILGSGERE
                     LEQEIEWMRAQMSENVGIYIGYSNELAHLIYAGSDFFLMPSLFEPCGIGQMIAQRYGT
                     LPIVRFTGGLKDTVCGYNGQNLGEANGFGFEHFDEYGMQYGLDLALSLYQDKEALLKV
                     RRNAMKLDRTWKNSALEYVKLYRDIKE"
     gene            complement(13402..14517)
                     /gene="glgD"
                     /locus_tag="PLH17_01025"
     CDS             complement(13402..14517)
                     /gene="glgD"
                     /locus_tag="PLH17_01025"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOR20396.1"
                     /translation="MASKVLGLLDLHNSPDLGPLTSSRVLASTSFLGRFAFMDFALSN
                     FSNSGIDEVGILVKSHLRSVLKHMGSMQSWNINTKIGRQTIMFNERGNMNRKKNTDIN
                     NFLVNDWVLHESKAKYIIIQPAHVICKIDFEPILKEHIARDEKVTIIYKHITNAHKSF
                     ATHNLVNVRNGYVRSFKRNRQLEKDACVSMETYIINRDVLLNLVSEALKTNPMYSFKD
                     AIKKYANTLFKVHAVEHLGYARSFDSLQHFVDYSFELLDYRYAHLLFIKDWPIYTITR
                     DTTPTLYGTKANIHNCFIANGAIVEGRVENSIISRNVKVGRGAIIRNSIILSDTVVGE
                     NAIIANAVIDKYCLISDSVHVEGVKGSPKYINQGKRL"
     gene            complement(14507..15646)
                     /locus_tag="PLH17_01030"
     CDS             complement(14507..15646)
                     /locus_tag="PLH17_01030"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOR20397.1"
                     /translation="MNKRIVAMILAGGKGTRLESLTRKIAKPAVSYGGKYRIIDFPLS
                     NCANSGIDTVGVLTQYESVALDTYIGNGEKWGLNGVRSLTTTLHPRQTEEGLSWYRGT
                     ADAIYANLDFLDSVSPQYVLILSGDHIYSTTYNQMLKQHIERGADCTISVIEVDPKEA
                     SRFGILVTDEKGAIVKFQEKPKNPISNLASMGVYIFTYSVLRRMLVGDARKKDSSHDF
                     GKDIIPTMLAKKMKLFAYVFKGYWKDVGTISSLHEANMDLLLSSKGQNIYTIKGGNTI
                     FSEDTHSHPQYIGRKARVKDCLINQGAVILGDVERSVISNEVVVAEGAVVKNSVLMPG
                     ARIRAGAKVFSAIVGPEAIVSHGEVVNEKQEDIVLITTRRKNNGK"
     gene            15779..16249
                     /locus_tag="PLH17_01035"
     CDS             15779..16249
                     /locus_tag="PLH17_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20398.1"
                     /translation="MNNRKLKISSFRLTFLFAGVYLFLQILFMSTFNFLDVTVWPPKI
                     VNLTLFYVYTPILLAVTIFFYVLSLTQTFYYVDKKKITRHKMNKIFEYYFSDMTYIDE
                     KWSKKHRTLLFYDKSGRRNFLTFDREGIIFQHALENARPLSREDFAIRFPDVRL"
     gene            16340..16528
                     /gene="rpmB"
                     /locus_tag="PLH17_01040"
     CDS             16340..16528
                     /gene="rpmB"
                     /locus_tag="PLH17_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOR20399.1"
                     /translation="MSRVCPVLGKGPLSGNNRSHSLRATRRRWNVNMQPYQVEIDGKV
                     QTVRMSARAHRSLMKSGK"
     gene            complement(16577..17251)
                     /locus_tag="PLH17_01045"
     CDS             complement(16577..17251)
                     /locus_tag="PLH17_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20400.1"
                     /translation="MGLWIATFVVLLFAFLGILIFGFSNYQETFKTKFSILNMFPYEL
                     SYHNQGMMIVFYRFFLYLYAAFSIAPALLMISKYYHFPGYVSYLIMIGILFVISAVSF
                     LTLHIIQAKYVRLHTLVATAYFAVSALAAGAVSIILLNLYISNNRVDFKFLLIGILEA
                     MLGFATLLIMLNPRLRNWANMNAKTEEDGTVVIVRPRFFILAFSEWLVIFINLATQTL
                     LLIAYL"
     gene            complement(17254..17907)
                     /gene="rpe"
                     /locus_tag="PLH17_01050"
     CDS             complement(17254..17907)
                     /gene="rpe"
                     /locus_tag="PLH17_01050"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOR20401.1"
                     /translation="MKNYVAPSLLAADKQRLSEEIKLAEESGAEYLHFDVMDGKFVPN
                     VSFGLEELEKISKLHKMVNDVHIMIENPDEEAIKYAKAGADIVTFHIEALPCMGCLAK
                     TIKALHDYGTKVGISIKPGTKVNALDPFLQDIDLVLIMSVEPGKGGQPFMKSALRKIK
                     YLRRMIDRHHYDVLIEVDGGINDVTGALAREAGADILVAGTYLFGHEDFKERVNKLR"
     gene            complement(17904..18773)
                     /gene="rsgA"
                     /locus_tag="PLH17_01055"
     CDS             complement(17904..18773)
                     /gene="rsgA"
                     /locus_tag="PLH17_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOR20402.1"
                     /translation="MKQGIIISAYGGKFVASADDESYDLSPRGAVRYKSGEVLPGDRV
                     LFDEGTSTIEKILARENKLPRPRVSNISQIMVMASAVEPEFSPLLIYKTLAYALMYDI
                     KPFVVISKIDRLDNLLEMSRFQHQLAQIGFEVILYSKKSKAGLLELKAKLAGQVTLAI
                     GQTGVGKSSLINLLDVNFKRAIGDYSKALGRGKHKTKEVIFLPFSDGFIVDSPGFSSL
                     ELDLYKEDLATFFPGFSQAVGKCFYRDCMHINEKGCEVKKMIALGTLSQEAYLIYQKL
                     LDELQYKNRRFQK"
     gene            complement(18770..19648)
                     /locus_tag="PLH17_01060"
     CDS             complement(18770..19648)
                     /locus_tag="PLH17_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOR20403.1"
                     /translation="MIKIGDKIDDRYRITSRLGTGGMSEVYEATDVISKRVVAIKIMK
                     EELMNDYENIRRFKHEVAAVASLQHPNIIKVYNNGEVNGRPYMANEYVKGQTLHEKLL
                     FLTKMQPYEACEIMLQILSAAIYIHNHGVIHRDIKPHNVYYMADGTIKLGDFGIATNE
                     EMSNANPDEHILGSVHYLAPELCQGKKPTPLSDIYALGITFFELVTGHVPFDEALPVD
                     IAVAHIKRPFPMPSKVDPSISREVEKIIIKACRKNPIDRYQSAQAMADDIENVMKNRN
                     NFKEKKSFLSRIFGFK"
     gene            complement(19632..20393)
                     /locus_tag="PLH17_01065"
     CDS             complement(19632..20393)
                     /locus_tag="PLH17_01065"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOR20404.1"
                     /translation="MMRFKGNFAYRIDIGRVRVTNEDQASVLINADGDVLMVVCDGMG
                     GHNKGDYASRTAVSVLAEAFKNKPKFIHPFFARRWLSSQIHRANNEIYDAAFSNDAYK
                     DMGTTLVAALILEDHIIIANIGDSRAYSVRHEGMKRLTSDQTYVDYLYRTGKISKDEI
                     ATHPKRHVLMNALGIYPSLTLDITSAPYIGFSILLCSDGLYNNISEKEMHAILTTEER
                     PEQKVETLIKVANNNGGSDNIAIAYWEVIKNDQNR"
     gene            complement(20390..21424)
                     /gene="rlmN"
                     /locus_tag="PLH17_01070"
     CDS             complement(20390..21424)
                     /gene="rlmN"
                     /locus_tag="PLH17_01070"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004027752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOR20405.1"
                     /translation="MNNIYGISHEKLVQAMLAKGQKSYRATQLFSWIYEKKAQSFDEM
                     SDVSLSFREVLKGEYYFEVPKIYKTQKSEDGTIKLLLELGDGHKIETALLRYNYGNAL
                     CVSSQVGCNMGCSFCASGLLKKRRNLEVDEMVGQILIMNALLNEEGKGEVVTHIVVMG
                     TGEPFDNFENVMDFVRIANHPKAFAIGARRITVSTCGIVPRIYEYADVGMQVNLAISL
                     HASNDALRSKLMRVNQAYPLDKLIEAVKYYVFKTGRRVTFEYILLSGVNDAVAYADEL
                     SDLIRGIEAYVNLIPYNAIDEKGFRRSDDIAVKAFQNQLSKRGITSTVRKEFGADIDA
                     ACGQLRAKEK"
     gene            complement(21417..22685)
                     /locus_tag="PLH17_01075"
     CDS             complement(21417..22685)
                     /locus_tag="PLH17_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20406.1"
                     /translation="MKALEIVKNVLNQILYQDVEFQHALRQAFKDHPEDKEHSSLVSA
                     IVGCELRHHYLFQRIVSDLGTDYTDEQKCLLYASLANNLFLKRIEHDEMIDYVRQAFN
                     EEQMIIIEKLLAFQESPRELIPESIPVESYEYLSLRFNTPEWLVKMWQKHFGRGVTYK
                     LLKKNNKPQLQSCRVNTLATSTKEVLDDNPEFREAPLVDMVIYQGKAPIRKNTFFQSF
                     DVFLLKMGHKELMDKVCLNNVTEAFLYSAEDNSVIREFFLKYQKTIGLNLGVPNVDER
                     LDISKIIRYNQLTNVNFFGAEPTAMKAAISRPQDLVIVHPRSSDFDLIRAYPDYLLHF
                     KRESIDELVAKQKETLENCATFVGDDGILVYLVSTINRKEGHSLIMDFLDRHRDFALL
                     EEKQRFPFDPLDTALFYAIMKRKVEALPSE"
     gene            complement(22773..23213)
                     /locus_tag="PLH17_01080"
     CDS             complement(22773..23213)
                     /locus_tag="PLH17_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20407.1"
                     /translation="MITISILGLDQYLVGTYSEENTKKLADLYEVKPSEISFYSPNAF
                     YFHEGFDQTSWNALVRIHAPQECEVLEDKVTAYFFKTLKNIAIHISVEYFYYDKKHRH
                     TYINFDYPRFITEQNAVVVDEPLEDEEEDIYDGNIFAELDDRNK"
     gene            complement(23270..25465)
                     /gene="priA"
                     /locus_tag="PLH17_01085"
     CDS             complement(23270..25465)
                     /gene="priA"
                     /locus_tag="PLH17_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOR20408.1"
                     /translation="MRILEVLTEHRVNALNRPFSYVYFGRKEVRVGFRVLINFNRREL
                     VGYVIKVEESDLSLADYETEKGFTLSEIIDVIDDSELLNDELMNLVERICDHYLVTKI
                     SVLQTMLPKSLKPRLSALSGPKAYYEKYLVAIDDNEAGLTARQIDLLRLVKKNGKVLK
                     RDAGSPSIVNKLIATKHLKIIEIERSRLEIPEYQKETVKILNDEQKSAVKSILETDKT
                     VSLLQGVTGSGKTEVYLALSEAIIASGKNVLMLVPEIALTPIMVEYFQRRFKGDVAIL
                     HSELTEAEKYDEYRRIAKGETRVVVGARSAIFAPLENIGLFILDEEHVESYKQDTTPF
                     YHAREIAIMRAEYHHAKVVLGSATPSLESKARALKGVYHYAELPHRINDQVLPKVTII
                     DLSNPRSLTRQSVLFSELLIQKINDRLDKKEQIVLLINRRGFSGYVTCRSCGHIFKCP
                     DCGITLTYHREDEMLKCHHCSHVEFAPTSCPECGGTYFSKSGFGTERITSEIAKLFPR
                     AKTLRLDSDIGRVRNTIAKTIEAFRNKEADILVGTQMIAKGHDFPDVTLVGIVLADIG
                     LSLPSFRSSERAFELITQAVGRSGRAAKVGEAIIQTYNPTHYAIRLAAAQDYETFFKK
                     EMLARRAQQYPPYTFLTSVQISSKNEALAIDTIHAIAEDVQKSNYPDVTILGPVSPFI
                     HFEKEVHHRVLLLKYKSDVEIKNHLRKLLVALQSKGQINVAINVDPYNF"
     gene            complement(25478..26077)
                     /gene="gmk"
                     /locus_tag="PLH17_01090"
     CDS             complement(25478..26077)
                     /gene="gmk"
                     /locus_tag="PLH17_01090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOR20409.1"
                     /translation="METATPKEGLLIIISGPSGVGKGTIRRNVMGDDSLNLVYSVSMT
                     TRSRRETEVDGVDYFFVSREEFDRNIKAGNFLEWAEFVGNRYGTPKHYVDELRAQGKN
                     VVLEIEVEGTKQVLSRCKGSNVVSIFVIPPSYEDLEKRIRHRSTEPEDVIQKRLQKAR
                     KEMHLKYHYEYVVLNDRPDRAGKEIREIIRNKINAKIIK"
     gene            26143..27546
                     /locus_tag="PLH17_01095"
     CDS             26143..27546
                     /locus_tag="PLH17_01095"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOR20410.1"
                     /translation="MRISLQDFSQVVDIIAKEATGKRIRKVIFYSSRIFYLQISSGRK
                     RYLVINLDNTNPSIFLGELSNTPPTLVNPLYLFLKKELNNALIKKVEQQNNDRVVRIE
                     LEITNSAYKTFTRNLYVELIATRPNLIFVDEDEKILNAFRKNTIDSPRIVARGFKYEK
                     PLNGAKSKNGSAEPFDHTKFLNFELSRLNQSAMTRNQEKFVKIYKFINTRIRTASRKI
                     DNIKADVQRAEEQLINKEYGDAVLAHPADKKWRTDKIEANGIIIRLDGLITLAENAQH
                     FYKLYKKAKNTIAISDKLIEKAEKELNESLFIKDFLSSSTEEEIERFVAQNALISPRD
                     KIRLPVTKKYAPYHLENNGTTFYFGKTADQNDYLSFAYAARQHIWFHLLNQPGAHVVI
                     KKSNPTDEDLIVAAEIALLCSSLDRGEVMYASKKDISRGESKGEAHVKKYETIYISFI
                     RPATEALYKAATRLEVK"
     assembly_gap    27684..27783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27784..28188
                     /locus_tag="PLH17_01100"
     CDS             <27784..28188
                     /locus_tag="PLH17_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOR20411.1"
                     /translation="YERKTIASVRRFYDEDPFVLCHNDVVNGNLLFRANKVFIIDFEY
                     AADNNPLFDLASFLSENDIEDERLRRLFLKTYFGRRCEERIYRRLTRIIAVQDILWYY
                     WAQMHYVSGKELIYKRIAHHKWKAIMKSASSH"
     gene            complement(28216..28764)
                     /locus_tag="PLH17_01105"
     CDS             complement(28216..28764)
                     /locus_tag="PLH17_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOR20412.1"
                     /translation="MNVWHMAPRERIKPEQFLACIEISKGSKNKYELEKQTGALILDR
                     ILYTSTHYPQNYGFIPLTYAEDLDPLDVLVLSSESIVPLALVDCYPIGVLEMIDGGYL
                     DEKIIAVCKNDPIYNIYKDIRELPPHISNEIRHFFRVYKQLENKKTIVNNMKGAKHAR
                     EVIAKSLKAYVDKFVEYEPFAY"
     gene            28905..29747
                     /locus_tag="PLH17_01110"
     CDS             28905..29747
                     /locus_tag="PLH17_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20413.1"
                     /translation="MKMFKASLLVLIPLLLSSCGNRPRGLVLISQNGSLYDIQEPTAT
                     SELSDYVRHTDSPEILSYLQNDEPFVLYVGMAGCLGCNMFKPNLQRYVYETQALIHYL
                     NVGDNDDYLEYSKIWTGYQDIFMADLEVPYLMIVESDEAYVKGAVSKMTATTVEPFIN
                     MMNSLVDVANVASHIQNDSATNYLASSEEGLFFFYDRDDEDALEIYRTLIWPYAAKSE
                     RSLQVVDYTNFTEEELIVLRATFSLGEDIGPIAKYYEGEAMIEAHYFGIDEASDRQFL
                     EAYL"
     gene            complement(29777..30982)
                     /locus_tag="PLH17_01115"
     CDS             complement(29777..30982)
                     /locus_tag="PLH17_01115"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOR20414.1"
                     /translation="MKKAMVYIQSGGPTAVINTSLYGAVVEAKKYPDKIDKIYGSLYG
                     VEGLIIDDLIDLGQEDDETIELLKQTPGAALGSTRYKLPKDLHDKQYMSIIETLKKHN
                     IGYVFVNGGNDSMDTCHKLSLLCREIGYDCMVMGVPKTVDNDLALTDHSLGFASAAKH
                     VVNTVKSICVDARVYKRGKVYIIEVMGRNAGWLTASVDLLPEETRPDLIYLPENPFSV
                     DDFLRDVKKLFDKKGNAIVVISEGISFERDMTKARVDAFNHIQLGGVGDELADMVEVR
                     LDLPTRSIELSLPQRADPLLVSKVDRQEAIACGTIAVQLALQGHTGEMVCIKRLSDEP
                     YRVEYVPEDIAKIANAERVVPPSMMAGPSHMDESFRRYCLPLIQGSVDIKFENGMYKS
                     AVLKRIKVK"
     gene            31336..32985
                     /locus_tag="PLH17_01120"
     CDS             31336..32985
                     /locus_tag="PLH17_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOR20415.1"
                     /translation="MAFTLKFHDQEKSFDKKVALLDLVDDPQKEFVCAKVNNRIRELT
                     YEVYYDADVEFLTVRDADAIPIYETSLRYLVAMAFKRAYPELEVRFSYNVSRSIFVQI
                     LNDSRHSDMSMVRRIEDEMQTIVAADFPLNRMIVTKEEAKNIYEDKKFTDKLELLKYR
                     PEKTVHLYDCDGYLNYMYGRMVPSTGYLKKYKIRLYSPGIIIQYPRAEAGGDIPKFED
                     APTFGKTLKASHAWGKIAGADSVSGINEHIKKNGVIDFINMCETRHNRMLVELGNMIE
                     SDIDTIRLICIAGPSSSGKTTFSNRLRIELLSRGIKPIRLSIDDYYLPKSEVPKDENG
                     DPDLESINALDIELFNQNMLDLISGKEVQLPRFDFKLGHRVPGRKLKVSFSQPIIIEG
                     IHALNDMLTINIPKHQKFKIYISPQAQINFDNHNPMSLTDLRLLRRIVRDKKYRNASA
                     EETMSMWPSVRKGEFTWIYNTQEGADFVFNSLLSYELSVMKKYAMPLLSNIDTNSVYF
                     PVAERLIRMLKYFDDMPDEWVPSNSLLREFIGGSCYANVDV"
     gene            33091..33786
                     /locus_tag="PLH17_01125"
     CDS             33091..33786
                     /locus_tag="PLH17_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOR20416.1"
                     /translation="MKDFHEFDARLEKNFGIFSPAELSKIAKTRVLIVGLGGIGGYLA
                     NALARLGVRQLTLVDFDKFKVTNLNRQLFSSLKTINKFKVDIIKDELLKIDPTLKIDS
                     QPLSIEALDEDVVSKVDVVFDAVDVIKTKIYLEKMCSKYNLPLIHGALGGWFGQVGIS
                     LPKSSLLENFYGQDVSGLEETMGAPTFIPPLVAHYMVVQFILLCLGKKEALINQILNI
                     DALHTELSVVYKK"
     gene            33795..34043
                     /locus_tag="PLH17_01130"
     CDS             33795..34043
                     /locus_tag="PLH17_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOR20417.1"
                     /translation="MVKIIFFNLLRSKYHWHELDVAPGTFQNVIDELKKNNPSLDEKD
                     FRDCVMFVNDVRITHLARFDIQFKDGDKVVLTHFLGGG"
     gene            complement(34052..35179)
                     /locus_tag="PLH17_01135"
     CDS             complement(34052..35179)
                     /locus_tag="PLH17_01135"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOR20418.1"
                     /translation="MSKQEVISFEQFQDIYKALGVKESLDSQFLYDVYQNAMEGAKSI
                     DEANLLGRVVPVNPIALILYIVNEHGYYLDIHPDAAQEAVIKDEKYMQMIVSIALDKY
                     YTNEHLSYKSKTILSRFSPGISTLNTYLNFVLGVLGRYSRNQPNETLIVDIMSKGFSM
                     ARAISDLLVSGFETEAFSTWRTLHEAECILLVLTKYGKPVIDKYLLHMNYAMAFRGLA
                     GDKEKTDAIFTQIKGEMRTFDLKSKDMKRFIEYGWLLKVSALDKAEPVKLNFRDGLEK
                     TAGLKRYAPIYEMSSEIAHSSPLLIYSRREYFMHLALVNVYESFFRLEKIFAEIYVRS
                     ISESEKQRFLLMRKIYYGQLVSLHAQERAKMRKMAGHSKKQ"
     gene            complement(35182..>36557)
                     /gene="glpK"
                     /locus_tag="PLH17_01140"
     CDS             complement(35182..>36557)
                     /gene="glpK"
                     /locus_tag="PLH17_01140"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOR20419.1"
                     /translation="YPKPGWVEQDALQLWVSVIDVVNELLVISHKTMDDIAAIGITNQ
                     RETTIIWDRKTGRPIYNAIVWQSRQSLPYCQKIAKYRDLIHKKTGLLINPYFSASKVR
                     YILDKVAGAQKRAEAGELMFGTVDTWIIYNMTKGKVHATDPSNASRTMFFNIFDMKWD
                     DELLTLFNIPKSLLPEVKPSSADYGIASFFSKNIHIYGVCGDQQAALFGQACFIAGDL
                     KNTYGTGCFALMNTGKEAVLSQNGLLTTVAWQIGDEVNYALEGSVFVGGAVVQWLRES
                     MNIITTSDESERVARKVDDSQGVYIVPAFVGLGTPYWDNEARGAVFGITRGTNKSHFV
                     RASLEAIAYQSKDVIEVMKQEAHADVSALRVDGGATKNKLLLQFQADILRCEIRLPQC
                     LETTSLGVAYMAGLGSGFWKNMKEIEKYHCLQATYQPKMDEKVAIKKYEGWKQAVQAT
                     MMFKLKD"
BASE COUNT         9532 a         8459 c         8573 g         9893 t
ORIGIN      
        1 aaagactaaa tgcgactttc gattataaag ttgcgtttag tttttcaatt ggggcttttt
       61 aattaaatat taaggttgcg tccgccacca tttctttttg ttgactaatc ggcgtctttc
      121 ctctatagtt atcgttgcat gtaatgcagc atgtaaatac gccttgtccg atgtcgggaa
      181 caaatgtgat agagtagcca aagctcaacg gtgaaataat atcccggaca tccgaaggat
      241 ggaatttgca cctcttgctt tatattgcaa aatataaggg aaaggagaat agaacatgag
      301 attcactggt tcggattaca aacgctcacg ccggctcggt ttttcgacgc ttgaaacagg
      361 cgaagaatta cgcaagagat cttacggtcc cggccaacac ggcaatgccc gcaaaagaaa
      421 accttctgaa tacggaaagc aattagtcga aaaacaaaaa cttcgccata tgtacggagt
      481 taatgaacgc caattccgcc gcctcttcgc catcgccacc aagtcgagcg aagtcaccgg
      541 catcgctttc ttccgcattt tggaatcgcg acttgataac ctcgtcttta gaatgggctt
      601 ttcaagaacg cgccgcggcg ccaaacagct cgtcaaccat ggtcacgtca ccgtcaacgg
      661 taaaaaagtc gacatctcgt catatctttg ctcggtcaat gatatcatcg ccattcgcga
      721 atcaagcaaa aacttgaaag tcgtcaaaga agcgcttgaa agcaaaccgt cagccgtcgg
      781 atatgtcaag gtcgatgccg tcaaacttga aggcaccttc ttacgcgctc cagagcgcaa
      841 cgaattaccg caagaaatag tcgaagcgca gatcatcgag tactacaacc gcttgctgta
      901 acgctgcaaa gctaatagtc aatataaaaa gacccttatg ggtctttttt tcaagaaatc
      961 aactagcgaa cattgatgag atagtaattt ttcttgccgc gacgcaagac gtaatacttg
     1021 ccatgcaagg catcagcgcc ggtcaattgg atagtgccat cgattgcttt ttgaccattg
     1081 agggcgatgc ttccgccttt taaaaattcg cgggcttcgc gcttcgaact ggcggcttta
     1141 gctgcaatca ggacatcttc aagaatgatg ccggagtcga ctttaacttc caaatcgccg
     1201 agaacatcga ttagattttt ttcgctcatc gtcacgaaat cgcccgaaaa gagtgactca
     1261 ctcatcttga tggcttcttc ggcacgcgct tgtccatgaa tgattttgac aacttcaaaa
     1321 gccagcgttt tctgggcttg gcgaagacct ggatttgctt cgtgagcttt cgccgcagtt
     1381 tcaatctctt cgcggcttaa aaacgtgaat actttcaaat agcgaatcgc gtcagcatcg
     1441 ctgacattga taaagtattg gtagagttta taagggctgg taatttctga atcgagatag
     1501 agagcaccgc cttcggactt gccgaatttg cggccggtcg aatcgacgat gaggtggctt
     1561 gtcatcacgc cgactttggc atctccgccc tcgactttgc gaatgagttc gagcccgctt
     1621 gtcaaattgc cccactggtc gctgccgccg acttgaatgc ggcagccgtg attacggtag
     1681 agggtaagaa agtcgatagc ctgcaaaagg ttatatgaga attcggtata tgatattccg
     1741 ttttcaaggc ggctggaaat aatttcttta tttaacaaat agttgatggg aaaatatttg
     1801 ccgtaatcgc gcagatagtc gagcatgctg attttgctca gccagtcata gttattgacg
     1861 atgaggccgc gctttgaatc ctcgagatta ataaatctcg ccagctgagc gcgaatggct
     1921 tcggtgttct tttttaactc gtttccttcc aagaggttgc gctctttgga ttttccggag
     1981 ggatcgccga tcatgccggt ggcaccgccg acgacaggga taacgcgatg accggctctt
     2041 tgcaggcgca tcagaaggga gatcatgaca aagttgccga tgtgcatcga agccgcgctc
     2101 ggatcaaaac cgcaataaat cgtctgcggg gttgagagca actcgcggat ttctttttca
     2161 tccgaataat ctttgattaa accccggtaa ttgagttcat cgataatatt gaccattgtt
     2221 tactccttag tgctgttgcg cgcggtataa gtcgattcaa acttataagc tttttcgttg
     2281 agttcgccgt ttgataaatc aatcagcata tacatcgctc tcttgccgac ttcctgcatg
     2341 ttaatataga aactcgacaa agtcggacgg atgatttcgg catattttgt cccgatgatc
     2401 gagatgactt caacatcgtc tggaactttt aagccggaat cggtcgcggc attgacgatg
     2461 gcggcgccga tcgaatcgcg ataggcgata aaaaagcctt tgcgatgctt cttgaaatat
     2521 tccatgaaat cgagatatgt ccgctcagag gaatcgtcga cgttaaaaat gcggtacgga
     2581 acgccgattt cattatgaaa atcgatgaaa gctttttcaa tgcgagccaa aagacggccg
     2641 gcgttgtgaa cgtggagaaa gacggccggc ttcatatcgt ttttctcgta gcgagctttg
     2701 agcatacgct tgacggcgtg ctcgtagccg aaattgatgc agccgatgcg attgccggtg
     2761 atcttattgt tgatgacgat ggtcggaacc gaataggaat tgattttctc gatgtcttcc
     2821 ttatcgagct gatcatcaaa aatgatgacg ccgtcgacgt gggaggtgat gactttttca
     2881 accatcgcga tcgcatcgct tcttgaataa gaagtgacaa aaagcgtcag gacaaagcct
     2941 ttttccttgg ctacctcagt aatgccgtta agcatgttgg atatgtagac atagttggcc
     3001 gaagggatga cgatgccgat attggtcgtc acatttgtcg ccaaagcgcg agccaacccg
     3061 cttggtttat agccgagttt ctgaatggtg gcttcgactt ttaaacgcgt cgcctcggtg
     3121 acatttccct gtttgttgat gacgcgggaa acggtcgcca gcgacactcc gctgacttta
     3181 gcgacctcat agatcgtgac gcggtctttt aagtaatcca tatctttttc tcctatagcc
     3241 ccttcatagt atatgaaaat gttttcataa tcaacagcga cattttcata aaggaggccg
     3301 gttaatagat gggataagga actttttgca aacgccgcga agggatgtca cggcgacctt
     3361 ccttggcttc gggacggccg tcgagacgga ctaaatcgcc ggtgaagcca acggttgtgt
     3421 tgttgacaaa agccgtgccg acgccataca tgtcgacggg cacgccgagt ttaacccact
     3481 cggtaatctt ggtgacgtca aaactgctcg aaacaattat tttaacaaag ttaaaacctt
     3541 cactgtcaag cgcttttctc agcgcaaaaa tcaattcttt gcacactccg tgagcattga
     3601 aacccgacgt atctttgcca tcaaaatagt ggtcgatgag agcttttgaa gtatcgacgc
     3661 ggacggcctt caaatcattt tttagatggc gggccaagac gagcgaatca tggacgacat
     3721 tgttgttgta atcgatgagg gcggtaactt tttcgtgcgg aaaggttttt tgatagaggg
     3781 tggcggcttg aatgatatcg ccgccgcaca cttgaatgag ggcatgaggc attgtgccca
     3841 tgccgcgacc gccccaccag tagccctggg cgtcggtgct gactttatcg atgccggcga
     3901 cataggtggc atagccgtcg ccgacctgcg tcaggtaatc atcttggcgg tcggccatcg
     3961 aaaagcacgg cgttccgttt aacttggcga cgacgcggtg aacattggtc gcgacgctgc
     4021 tgcgccgggc gagaatgcca tcgatcatac tttcgagaaa accgaagttt tcatatttgc
     4081 cagtaatctt caaaacgggc tcgttggcgg cgatgatatc accgtcgttc aaggcttcga
     4141 tcaccaggtt ttctggttcg acggcaaagg tgtgaacgag ggcgatgact tcatccagtc
     4201 cgcacaccat cgcttcttcc gtcctttgaa aaaactgcat ggtgacgatg tgatggggat
     4261 tatgctcttt gacgatatcg cgggctttca gaaaataatt cgcggcataa aatccgcttc
     4321 taaggcgctc atcaaaagca aatgtcctgt tggtcaaacg cgagatctta ccttgttttt
     4381 ttaaaacgat ttccagttcc ataaatcact ccttggcaaa gaaatcttga ccgatttcca
     4441 taaattcttc aataatatac gttttctcat cgccttttgg cgattcaaga tttaaatctt
     4501 tatatgtgca gatgcggcct tcaaaataca tgccgtcatg agtctccacc accaccgtct
     4561 caccattaaa aagaagcgtg tcggcaaggg ctataacaac catggaatac ggcgcgattt
     4621 cttcgttttc agattttgta taaatgacgc gcaatccgat gtcgacttta gcgattttga
     4681 agggatgatc ttcgcattca ataagttcca aaatctgacc ggcgacaaaa cccgactcgc
     4741 tttgttcact caagggttcg agaccttcat tttttaagat atgattaatg acttcgagaa
     4801 actcttttgc cgggctcaca atcattcctt gcgctttgat gcggacgatt ttggagatgt
     4861 taaaaagatt gatgccaatg agctcataat cggaataaat taatgtgaca tcgtttcttt
     4921 tatcaaggtg agtcgcgcgc ttttgattgt taaaaacaat catcaagaca tcaccgagcg
     4981 tgtgataatt ataaaagagg cgatacatat cagcgcctgc ttccttttag attagcgagt
     5041 cgggaagcat caaaatggcg aaggatgtga agcagggact ttctggccgc taagtagtca
     5101 tcgacatcga tgattgaact cggcgaatga atgccccttg cgcagatgca atgcgtcagg
     5161 gtcagagttc cggaattagc tttgtgaatg ctgccggcat cggtgccgcc cggagaatca
     5221 aaatattggt atttgacgcc cgtttcgcgg catgcttgaa tctgcatgtc taaaagctcg
     5281 ggaaaggcga tcatcgcgcg gtcgagatag cgtatcaata ccccttcgcc taagcggccg
     5341 tattcatctt ttttcgcgcc gatatcgcga gccggcgaac aatctaagac gacggcgaaa
     5401 tcaggattga ttaagtgggc ggccgtctgc gctccgcgca atccgacttc ttcctgaaca
     5461 tttgccccga tataaaggtc ataaggcaaa tcttcgccga ttaactcatc gagcacatca
     5521 agagctaatg ctatgccgta gcggttgtcg aaagctttgg ctaataacct ttggccgtta
     5581 tttaggattt taaaatcgcc gaggacgacg attgaatcgc cgattgataa accggcggcg
     5641 agggcttctt ctttggatgt aaacccgaaa tcaaagagta actcttcgat gtatcggtct
     5701 tcgtcttgat ctttttcgct tttgaggtgg ggcggaatcg aagcgatggc gccatagtat
     5761 ttttttccgc tcttcgtctt taataagacg cgatgggcag ataacgtttc tttgctgtgc
     5821 ccgccgagag ctgaagtttt aattaaaccg ttatcttgaa tatataaaag catgaagccg
     5881 atttcatcca tgtgagcgga aaccatgact ttcggggcat ttataatctt tgatttttta
     5941 agcccgtaaa ggctcccgag gttatcgctg actagttcta aacccttgtc caaaaattct
     6001 tgcttaagaa aatttcgcac ttcgttttca tcacccgata ccgagatgaa ctgtgtcaca
     6061 ctttctaaaa gcgatagttg acgattggtt agtttcataa ttattttctc cttgcgacga
     6121 tgcggttgat atttttgctg aggtttctaa actgcttttc atactcgctc atcgcgtcgc
     6181 cttccgccag cgcggaataa ttgtccgtat aggcgataag ttccagcggg ctctctttga
     6241 tagtagctaa agagtagtca taaagggcgt cattatcgct tttaaaaaag agatatccgc
     6301 cctttttgag gatgcgagta tattctagca actttgcggg aaaagtgagc cgccttttct
     6361 cgtgcttttt cttcggccac ggatcggaga aattaagata gatggcacct aatgtctcgt
     6421 cgagaaataa ggggagaatc tttgcgacat catcgacgat aaagcgaatg ttctgcaact
     6481 tgttttccac cgcttttctg gccgccatcg cggcaaccat cgccgaaact tctatcgcca
     6541 aatactttgt attggggtta ttggtggcca aagcgacgat aaaatcgcct ttcccgcagc
     6601 cgatttccat ctcaagagga ccatccgcga aaaaggaagg atcgtcatca agtgatttaa
     6661 tcactatttc ttgatgatca tcgaggtagg gttttgccca aggtttgtag cgggtcctca
     6721 tttaagattt cccagcatgt ggacagcccg cgcataaatg gccatcgaga tgtaaaaatt
     6781 gtttaaaatc agatgctcat cagcttcgtg catgtgcgga ttgtcatcgg gaaaagcggc
     6841 cccgaaagcc acggtgctag gagcttcttt ggcatacgtt ccgccgccca tcgtcagcgg
     6901 tttgctgcgc ttatcgcccg tctcttcgcg ataggcgttt aggagtgttt taatcagcgg
     6961 cgatttaaga ggaaaaagaa gcgatggact ccgcgagtag atttcgctcg tcaggccgct
     7021 tgcctcatcg aaacggcgca caaaatcagc atcgtcaaca tcttctggat accggaaatt
     7081 gactttgagc tccaaagcct tcccgtcata ggcgatgacg ccgagattat aggtggtctc
     7141 gcccaagtct ttgctcttcg cataaccctt aaaaggtttg ccatcgaaat ggcgaaactg
     7201 agcaaagatt tttaagagga tatcgttttg ataaaaggtt cccagtgctt caatcgcaaa
     7261 aagagcggca ttgactccgg cttgcggagt cgaaccgtgc gcggacttgc catgaaaagt
     7321 aatctttgat acgccgttaa tgttttcgat agtcgtctta ttttttaagc ctttgagaaa
     7381 ggtttcaaac ttcgcatcgc ttttcatgac gacaagagtt gaatcaatca ccgagttaac
     7441 cgcctctccg ccgttaatct tcatgatgcc ggggaggacg agattgatgc ggctacggaa
     7501 attgctgata cctttttcgc catagataac cggaaaattg gcatcgggcg taaaaccgta
     7561 agtcggatgt tctttcttta aatccttaaa ataatatttt aagcagctgc tgccgcgctc
     7621 ctcgtctccg ccgaccacca ggcgcacgcg atagttattg atgaggttat tgtcttttaa
     7681 ggctttgata gcgtaaaaag cggcgatgag cggaccttta tcatcgctga cgccacggcc
     7741 gaacaccttg cctttttcaa tttgggcgcc gaagggttcg tgcttccatc cgcttccgac
     7801 ggggacgaca tcggcatggg caaaaataga gatgagtctt tcgccttggc caaaagtcat
     7861 ctcggtcgcg cggttgtcgc agtaatcgac atcaaagccg aactggcggg ccagacgccc
     7921 gacatattta agagcttttt caactccggc gccgtaagga gcggtgctcg aggcgctctt
     7981 ttcatcaaaa acggagttga ttttgatcag ctcttgcaaa gcccgcacgg cattcgcgcg
     8041 gtaagggttg gtaagttcaa tgaaatcaag gtcttgtttc attattttgc tcctttttat
     8101 taagcagaaa gggatatttg tggtagatag ggacggcaat cgcaggtgta attacggcag
     8161 cgataccggc ctcgataagc gccccaaaaa caatcgctag aagaccgata aaattaatcc
     8221 aatgaaacga ctgagcatct tgaatttgaa gtattcttaa cacatcagct ttatatacta
     8281 accatattga tgtaataacg agaaaggtat gaaatagggt catgaccaaa gaagtcgcag
     8341 ctataaataa gcctttttga tataggcgag gcatttttct taatagttca agcgaaaagc
     8401 cagctactaa accaaataaa gcacgaggaa gaactgatat taaaggatta ataaatagcg
     8461 gatcaaaagc acctcttggc tgagttgctg cttgaattaa cgttaataaa ccaaaggcaa
     8521 ttccgtatat taatcctctc ttccaaccaa ataaagcggc gccaattaaa acaggaatat
     8581 gaagtaaggt aaaagagatt aatggactca cagtaataag gccaatatta ggcacaaagg
     8641 ccataatagc tataatagcg atgaacatcg catcaaaaac gattgtccga ataattttat
     8701 ttctcatttt ttctggcctc gtttcgttcg tagatgacgc gaagtccgtc aagcaagaga
     8761 atttcatcat agatgaagtc ggccagcaaa tccttgacat aaatagcgtc gccaccagtc
     8821 agaatatgcc tggtcggata gccgagttcc tcttcgaatt ttttgacaag gccatcaatc
     8881 atgctcgccg ttccatagat tgcgccggca ttcgtcgagt ctttcgtgtt cttgccgatg
     8941 accttcttcg gtcgcgataa cgaaacgcga gaaagctgtg cggcgcggcc gattaaagca
     9001 tcgacggcga catagaggcc cggataaaaa gtcgcgccga caaaagcgcc tttgtgatcg
     9061 atggccaata gttttatcgc cgtacctaaa tcgacgatga tgagcggcga gccgtatttg
     9121 gcgatggcgc ccaccgcatc ggcgaccaaa tcgcttccca gttcgttggg atggtcgatt
     9181 aaaatcggca gtcccgtctt aagacccggg gtcaggacga tgctttctaa tttaaacgac
     9241 aggcgcagag cttcgcgcat cacttccgtt agaaacggaa cgacgctaga aataatcgct
     9301 ccgcgaaaag cgcttttgtc gatttttctg aattggataa aagtctcgat cgtcgagaca
     9361 tattcatcga gacttttctt aatatcggtc gcggttttaa aagaagccac caggcgggat
     9421 tgtttaaaaa ccccaaaggc aatgagggta ttccccacat ctaccgttaa taacatacgt
     9481 ttacctccgc tacaatcttg cttagcaagt atcgtgcaaa ttgattatag aaaacaattc
     9541 aaataaagaa aagagaatcg ctagcggacg acgatattga caatgcgccc tttgacgatg
     9601 atgattttga cgatttcttt tccttcgata tttctttgca ctccctcaag cgtcaaagcc
     9661 cgtttccgga gttcttcttc ttcagcctcg acatcggctt caaaggtgcc ccgcaatttg
     9721 ccgttgacgg atactgccat cgtcacctgc gagcggacga gtttgctctc atcgaaggtc
     9781 ggccacggag cataggtgat gccctcttga tggccgaggc tctcccatat ttcttcgccg
     9841 atgaaaggcg ccacgcagga aagcattttg acgaaacctt caagataggg gcgatagagt
     9901 ttatcggcct tataagcttc gttaacgaaa atcatcaact gcgcaatcgc ggtattaaac
     9961 tgcaaatttt ggtagtcttc gctaactttc ttgaccgtga agttgtagac atagtctaaa
    10021 gatccatcat tagtgtcgct gtgcttggcg aggaattcgt cttcgctaaa taagcgaaag
    10081 gcgcgttcaa gaaagcggcg cgctccgtcg aggccgtttt cagaccaggg cagggaggct
    10141 tcaagcggtc ccatgaacat ctcgtagagg cgcagggtat cggcgccata tttggcgatt
    10201 acatcatcag gattgacaac attgccgcgc gactttgaca tcttctcgtt attttcgccg
    10261 agtatcatgc cctgatggaa aagcttctta aaaggttcct cgcatttaac gatgccgaga
    10321 tcatacaaga atttgtgcca gaagcgggcg tataaaaggt ggagaacggc gtgttcggca
    10381 ccaccgatat acaagtcgac cgggagccag tgagctaaaa gctccgagtc gccgattgct
    10441 tgatcattgt ggggatcgat gtagcgcaga taataccagc atgagccagc ccattgcggc
    10501 atcgtgttcg tctcgcgttt gccttttcgg ccatcgattg taacttcgag ccattcgccc
    10561 gcatgaacaa gcggcgactc gccggtacct gagggtttgt attcgctgag cttcggaaga
    10621 atgagtggca actcgccctc ttggaggggt tcgattttgc catcatccat cagcgcaacc
    10681 ggtatcggct cgccccaata tctctggcgt gagaagagcc agtcgcgaag ccgataggaa
    10741 atttgaaatt ggccaagacc ttttttatta agatgagcga cgatgacttt tgtcgcttcc
    10801 tgattattta gtccgtcagc aaaggcgctg ttaatatgtt tggcgtcttc ttccatcgct
    10861 cttttagata tatcgccttc taagacttgg acgatgggta atttgtgttt atgggcaaat
    10921 tcataatcgc gctgatcgtg ggccggaacg gccatcacgg cgcccgtccc ataagttgcc
    10981 aagacataat cggcgatgta gatgggaact tctttttcgt taattggatt aatggcataa
    11041 gcgccgataa aaacgccact cttctcctta ttaagctcgg ttctttcaag atctgatttt
    11101 gcggcaattt gcgataaata tgcctcaatt gcgggtaatt tatcgggatt tgcaatcttt
    11161 ttgactaatt catgctcagg agccaggacg cagtaagtgc agccgtacaa tgtgtcggca
    11221 cgggttgtaa aaacatcaaa ggatgtgttc tgtcctttga ctttaaaggt gattttagcg
    11281 ccttctgaac gcccgatcca gttcgtctgc atgtcgatcg tcgactttgg ccagtcgact
    11341 tggctgagcc ccgagagcag gcggtcggca tacttggtaa ttcgcagtaa ccactgccgc
    11401 atcggcaagc ggatgacatc gtagccgccg cgttcgcttt tgccattgat gacttcctcg
    11461 ttggcgagaa cggtgccgag ttcggggcaa taattaacag gtatcgaatc gacataggct
    11521 aaatcttttt caaatagttt tagaaaaatc cactgcgtcc agcgatagta ggacgggtcg
    11581 gtcgtattga cttcgcgaga aaaatcatag ctcaatccaa tcgcctgaat ctgccgcctg
    11641 aagttattga tatttgcgat ggtaaaatct tcgggatggc ggttcttctt gatggcaaac
    11701 tgctccgcgg gcagcccaaa ggcatcccag ccaatcgggt gcaaaacatt aaaaccctgc
    11761 attctcttca tgcgggcaat cgtgtcgctg gccgtatatc ccttggggtg accgacatga
    11821 agaccgtccg cactcggata aggaaacata tccaggatat aatattttgg cttgctgaaa
    11881 tcggaagtat cagttttaaa aacgttgccg tctttccatt tttgagccca ttttttttct
    11941 atttcaagat ggttgaattt cattttttta ctccttgata tcgcgataaa gtttgacata
    12001 ctcaagagcc gaatttttcc aagtgcgatc gagtttcatg gcattgcggc gcaccttaag
    12061 caaagcttct ttatcttgat acaaggaaag ggcgaggtca agtccatatt gcattccgta
    12121 ttcgtcaaag tgttcaaagc caaagccgtt ggcttcgccg agattctgtc cgttatagcc
    12181 gcagaccgtg tcttttaagc ctccggtgaa acgcacgatc ggtaaggtgc cgtaacgctg
    12241 ggcgatcatc tgaccgatgc cgcacggctc aaaaagcgag ggcatcaaaa agaaatcgct
    12301 tccggcgtaa attagatggg ccagctcatt tgaatatccg atatagatgc cgacattctc
    12361 gctcatctgc gcacgcatcc attcaatttc ctgctccaat tcgcgttcgc cggagccaag
    12421 gatgatgatg gaaccgccgt tagcgacgat gtggcgcgct ttggcgatga ttaaacgaat
    12481 gcctttttgc caggtgaggc gtgaaactaa accaaagacg ggttgaccgc ggttcggaag
    12541 attaaactgc ttaagtaagg ccgctttatt ctcgtctttg cccttggtga cattaacgcc
    12601 gttaaagagg taggcgatgg cgcggtcggc tttcggattg aactcggcat aatcaattcc
    12661 gttgaggatg ccacaaaaat cctgacgccg aagctcgagg acggagctca gaccgtgttc
    12721 gctgtcaaaa gataaaagtt catcgcggtg agtataggag acggtcgcga ttttatcgga
    12781 gtagacaatc gcggctttaa gcgttgaaac aacattttca aagcggactt taccgctgtc
    12841 atataggctg tcgctcaact catagaaatt atttaaaaaa tacttatcaa acatgccttt
    12901 aaaggccgga ttatgaatcg ttaaaacaaa tttgaaattc tgaaagaaag ggtcattttg
    12961 gttttgttcc ctgatgatga cgggaagcat gccgggctgc cagtcgtgta ggtggacgat
    13021 atccgcttta aaaccgaggg ctttaaaggc gttgcgaacg gcaatcgtaa agaaggcaaa
    13081 acgctctaaa tcatcattat aaccataaag gttatcgcgg ccgaaataat agtcattttg
    13141 gatgaaatag aaagtgattc cgtcgacata agtgcggtac acttttgcgt attggcgccg
    13201 ccatgacatc gggacgtcaa actccgcgac gtgctgaagc ttgtcgccga attgagcgat
    13261 aatcgactga tagagcggca tgaataaagc cacctcttcg ccgagcatga cgagttcgcg
    13321 ggataaagaa taagtgacat cggcgagacc gccgcttttg acaaacgggt tggcttcgct
    13381 agtgaccatc gcaattttca ttcaaagcct ctttccctga ttgatgtatt tcggcgatcc
    13441 cttgacccct tcaacatgaa cgctatcaga aatgaggcag tacttgtcga tgacggcgtt
    13501 ggcgatgatg gcgttttcgc cgacgaccgt gtcggagagg atgatggaat tgcgaataat
    13561 cgccccgcgg ccgactttca cattgcggga tataatcgaa ttttcgacgc gtccttcaac
    13621 gatggcgcca ttggcgatga aacaattgtg aatgttggcc tttgtgccat agagggtcgg
    13681 cgtcgtatcg cgcgttatcg tgtagatggg ccaatctttt atgaagagca gatgggcata
    13741 acgatagtcc aatagttcaa atgagtaatc aacaaagtgt tgaagtgaat caaaagagcg
    13801 cgcgtaaccg aggtgttcga cagcatgcac tttaaataaa gtgttggcat actttttaat
    13861 cgcatcttta aagctataca tcgggttggt tttaagcgcc tcagatacca gattaaggag
    13921 aacatcgcgg ttgatgatgt atgtttccat cgagacgcag gcatcttttt ccagctgccg
    13981 attgcgtttg aaggagcgca catagccatt tctgacattg actaaattat gggtcgcaaa
    14041 agatttatgg gcattggtga tgtgtttgta gataatagtc accttttcat cgcgggcgat
    14101 atgctctttt aaaatcggtt caaaatcaat tttgcaaatg acgtgtgcgg gctgaatgat
    14161 gatgtatttg gctttgcttt cgtgtaaaac ccagtcattg accaaaaaat tgttgatgtc
    14221 agtatttttt ttgcggttca tgttgccgcg ctcgttgaac atgatggtct ggcggccgat
    14281 tttcgtgttg atgttccacg actgcatcga acccatgtgc ttcaggacgc tgcgcagatg
    14341 ggatttgact aagatgccaa cctcatcaat acctgagttt gagaagttgg aaagagcaaa
    14401 atccatgaag gcaaagcggc ctaaaaacga tgttgaagca agaacccggg aacttgtcaa
    14461 tggaccgagg tcgggagaat tgtggaggtc gagaagaccg agaactttac ttgccattat
    14521 ttttcctccg cgtggtgatc aaaacgatat cttcttgttt ttcattgacg acttcgccgt
    14581 gggaaacgat ggcttcggga ccgacgatgg cactaaaaac tttagcgccg gcacgaatgc
    14641 gggcccccgg catcagcacc gagtttttga cgacagcgcc ttcggcgaca acgacttcgt
    14701 tagagatgac cgagcgctcg acatcgccga ggatgacagc tccctgattt atgaggcagt
    14761 ctttgacacg cgctttgcga ccgatatatt gcggatggga atgcgtgtct tccgagaaga
    14821 tggtattacc gcctttgatc gtataaatat tttgaccttt gctagaaagc agcaagtcca
    14881 tattggcttc gtggagactc gaaatcgttc cgacgtcttt ccagtatccc ttaaaaacat
    14941 aggcaaacaa tttcatcttt ttggcgagca tggtcggaat gatgtctttg ccaaaatcgt
    15001 gcgatgaatc ctttttgcgg gcatcgccga cgagcatgcg ccgcaagacc gaataggtaa
    15061 atatgtagac tcccatcgaa gcgagattgg agatgggatt ttttggcttt tcctggaatt
    15121 tgacaatcgc ccctttttca tcggtgacga gaataccgaa acgcgaggct tctttaggat
    15181 cgacctcgat gacggatatc gtgcagtcgg cgccgcgctc gatatgctgc ttaagcattt
    15241 ggttatatgt cgtcgaatat atatgatcgc ccgataagat taaaacatat tgcgggctga
    15301 cggaatcgag aaaatctaaa ttggcataaa tggcatcagc cgtgccgcga taccagctca
    15361 atccctcttc ggtctggcgg ggatgaaggg ttgtcgtcaa ggagcgaacg ccgttcaaac
    15421 cccatttttc accgtttccg atataagtat cgagggccac cgactcatat tgcgtcagca
    15481 ctccgacggt atcgataccc gagttggcgc aattggataa aggaaaatcg ataatccggt
    15541 atttgccacc atagctgacg gcaggttttg caatttttct tgtcaaagac tcaaggcgcg
    15601 tgcctttgcc gcccgcaaga atcatcgcca cgattctttt gttcatcttt ttacctcgcg
    15661 attatgtcga ttaagcatca tcatgatgcc ctatcaaatc ttttataatt ttatcacata
    15721 tattcatata tatgtagtct cgttttaatc ccgagggcga tttgttataa tgattatcgt
    15781 gaataaccgc aagctgaaaa tatcatcgtt tcgtttgaca tttttgttcg ccggcgtcta
    15841 cctttttttg caaattctct tcatgtcaac cttcaatttt ctcgatgtca cggtctggcc
    15901 tccaaaaatc gttaatctca ccctatttta cgtctacacg cctattcttc tcgcggtcac
    15961 gatatttttt tatgtcctct cactgactca aactttctac tatgtcgata aaaagaaaat
    16021 aacccgccat aagatgaata agattttcga atactacttt tcggatatga catatatcga
    16081 tgaaaagtgg tcgaaaaaac accgcactct gctcttttat gataagtcag gacgtcgcaa
    16141 ttttttgact ttcgatcgcg aagggatcat ctttcagcac gcccttgaaa atgcccgtcc
    16201 tctcagccgc gaggattttg cgattcgctt tcccgatgtc aggctttaaa tgatactaca
    16261 tgcgcagtaa aagtgtttgc atccattatt aacttatgtt aaactcaaac gtgcgtttta
    16321 agaaaatgag gtcccagtta tgtcaagagt atgtcccgtc ctaggtaaag gtccgctaag
    16381 cggcaacaat cgttcccaca gccttcgtgc cacgcgccgg cgctggaatg tcaacatgca
    16441 accataccaa gtcgaaatcg acggcaaagt gcaaactgtt cgcatgtccg cccgcgctca
    16501 tcgcagtttg atgaaatcgg gaaaataacc tttcatcagt taaaaaagta agccccaaat
    16561 cggggctttt tctttgctat aaataggcga tcaacaaaag cgtctgcgtc gccagattga
    16621 taaagatgac gagccactcg ctaaaagcta agatgaaaaa gcgcggtctg acaatcacga
    16681 cggtgccgtc ttcttcggtc ttcgcattca tgttcgccca attgcgaagg cgcgggttga
    16741 gcatgattag caaggtcgca aaaccgagca tggcttccag gatgccgatc agcaggaact
    16801 taaagtcgac acggttgttg gagatatata gattaagaag aataatcgag acagccccgg
    16861 ccgccaaggc gctgacggca aaataagcgg tggccactag agtgtgcagg cggacgtatt
    16921 tcgcctgaat aatgtgaagc gtcagaaaag atacggccga aatcacaaag aggatgccaa
    16981 tcataataag ataagagacg taaccgggaa aatgatagta tttgctaatc attaaaagag
    17041 ccggcgcaat tgaaaaagcc gcataaagat atagaaagaa gcggtaaaag acaatcatca
    17101 tcccctgatt gtgataggag agttcatagg gaaacatgtt aaggatagaa aacttcgttt
    17161 taaaggtttc ctgataattg gaaaagccaa aaatcaggat gcctaaaaaa gcaaagagca
    17221 aaacgacaaa agtggcaatc cacagtccca taactatctc agtttattaa ctctttcttt
    17281 aaaatcttca tgtccgaaca aatacgttcc ggcgacgaga atatccgcgc cggcctcacg
    17341 ggccaaagcc ccggtcacat cattaatgcc gccgtcgact tcgatgagaa catcataatg
    17401 gtgacggtcg atcatccgcc gcaaatactt gattttgcgc aaggcgcttt tcataaaggg
    17461 ctggccgccc ttgccaggtt caaccgacat aatcaaaacg agatcgatat cttgcagaaa
    17521 tgggtcgaga gcgttaactt ttgtccccgg cttaatagag atgccgactt ttgttccgta
    17581 atcgtgaaga gctttgattg tcttagccag gcaacccatg cacggcaagg cctcgatgtg
    17641 aaaggtgacg atatccgctc cggctttagc gtatttaatg gcttcttcat cgggattttc
    17701 tatcatgatg tggacatcgt tgaccatttt atgaagcttg ctgatttttt ctaattcttc
    17761 gagtccgaat gagacgttgg gaacgaattt gccatccatc acatcaaaat gcaaatattc
    17821 ggcaccgctt tcctcggcga gttttatttc ttcggacaac ctctgcttat cggcagctaa
    17881 aaggctgggt gccacatagt ttttcatttt tgaaacctcc tgtttttata ttgtaattca
    17941 tcgagcagtt tttgataaat caaatacgct tcttgactca atgtcccgag agcaatcatc
    18001 ttttttacct cgcaaccctt ctcgttaatg tgcatgcaat cgcggtaaaa acacttgccg
    18061 accgcctgcg agaaaccagg aaaaaaggtc gcgagatctt ctttatataa atcgagttct
    18121 aaagacgaaa aacccggtga atcaacgata aaaccatcgg aaaaaggcag aaaaataact
    18181 tctttcgtct tgtgtttgcc gcgtcccagg gcttttgaat aatcgccgat cgcccgctta
    18241 aaattgacat cgagcaaatt gatgagcgat gattttccga cgccggtctg gccaattgcc
    18301 agggttactt gtccggctaa tttggctttc agttcaagga ggccggcttt tgattttttg
    18361 gagtataaaa tgacctcgaa accgatctgc gctaactggt gctgaaagcg gctcatctcc
    18421 aagagattgt ccaatcggtc gattttgctg atgacgacaa aaggtttgat atcatacatc
    18481 agagcgtacg ctaatgtctt ataaataaga agcggggaaa actccggctc caccgccgaa
    18541 gccataacca tgatttgcga gatattgctg acgcgaggac gcgggagttt attttcgcgg
    18601 gcgaggatct tttcaatcgt cgatgtgcct tcatcaaaaa ggacgcggtc gcccggcagc
    18661 acttcgccgc ttttatagcg aacggcaccg cgcggcgata agtcatatga ctcgtcatcg
    18721 gcgctggcga caaatttacc gccataggcg gagatgatga ttccctgctt catttaaatc
    18781 caaagatacg cgagagaaaa ctctttttct ctttgaagtt gttgcggttt ttcatgacat
    18841 tttcaatgtc atccgccatc gcctgggcgc tctgatagcg gtcgatcggg tttttgcggc
    18901 aggctttgat gatgattttc tcgacctcgc gggaaatcga gggatcaacc ttggacggca
    18961 tcggaaacgg tcgtttaatg tgggcgacgg cgatgtcgac gggcaaagct tcgtcaaacg
    19021 gaacatggcc ggtaacgagc tcaaaaaagg tgatgcccag agcatagata tcgcttagag
    19081 gggtaggttt tttgccctgg cacagctccg gagcgagata gtggacactc cccagaatgt
    19141 gctcatcggg attggcgttg ctcatctctt cattcgtggc gatgccaaaa tcgccgagct
    19201 taatcgttcc gtccgccatg taatagacat tatgcggttt gatgtcgcgg tgaataacac
    19261 cgtgattgtg aatgtagatg gccgcgctta atatctgcag catgatctca caggcttcat
    19321 agggctgcat ctttgtcaaa aacagcaact tctcatgaag ggtctgccct ttgacgtatt
    19381 cgttggccat atagggacgg ccgtttactt cgccgttatt ataaactttg atgatattgg
    19441 ggtgctgcaa gctggccacg gcggccacct catgtttaaa gcggcgaatg ttctcatagt
    19501 cattcatcag ctcttctttc atgattttga tggcgacgac gcgctttgaa atgacgtcag
    19561 tcgcctcgta gacttcggac atgccgccgg taccgaggcg gcttgtgatg cgatatcgat
    19621 catcgatttt atcaccgatt ttgatcattc ttgatgacct cccagtaagc gatggcgatg
    19681 ttgtcggagc cgccattatt gtttgcgact ttaatcagtg tttcaacctt ttgttcaggc
    19741 cgttcttcgg tcgttaaaat cgcgtgcatc tctttttcgc tgatgttgtt ataaaggccg
    19801 tcgctgcaaa gcagaatgga aaagccaatg tagggcgcgg atgtgatatc cagcgtcaga
    19861 gacgggtata ttcctaatgc gttcatgagc acatggcgtt tggggtgggt ggcgatttcg
    19921 tctttgctga tttttccggt gcgatacagg taatcgacat aagtctggtc gctggtcagc
    19981 cgcttcatgc cttcatggcg aaccgaatag gcgcgcgaat cgccgatatt ggcaatgatg
    20041 atatgatctt cgagaattaa ggccgcaact aaagtcgtgc ccatatcttt ataggcatca
    20101 tttgaaaagg cggcatcgta aatctcgttg ttggcccgat gaatctgact ggaaagccat
    20161 cttctggcga aaaacggatg aatgaatttt ggtttgtttt taaacgcctc ggcgaggacg
    20221 ctgaccgcgg tgcgcgaggc atagtcgccc ttgttgtgac cgcccatccc atcgcagacg
    20281 accattaaaa catcgccgtc ggcattgatt aaaacactcg cctgatcctc gttggtgacg
    20341 cggacgcggc cgatatcgat gcgataggcg aaattacctt tgaatctcat catttctcct
    20401 tggcccggag ctgtccgcaa gcggcgtcga tatccgctcc aaactccttg cgcaccgtcg
    20461 aggtgatgcc gcgtttgctc agctgatttt gaaaagcctt gacggcgata tcgtcgctgc
    20521 ggcggaaacc tttttcatcg atggcgttat aggggatgag gttgacataa gcttcgatgc
    20581 cgcgaattaa atccgacaat tcatcggcat aggccacggc gtcattcacg ccggacaata
    20641 aaatgtattc aaaagttacg cgccggcccg ttttaaagac ataatacttg accgcttcga
    20701 taagtttatc caaaggataa gcttgattaa ctcgcatcaa tttactgcgc aatgcatcat
    20761 tggatgcgtg gagggaaata gcgaggttga cctgcatgcc gacatcggcg tactcgtaga
    20821 tgcgcggaac gatgccacaa gtcgagacgg tgatgcgtcg cgcgccgatg gcgaaggctt
    20881 tcggatgatt ggcgattctt acaaaatcca tgacattttc aaaattgtca aacggctcgc
    20941 ccgtccccat cacgacaatg tgggtcacga cttcgccttt tccttcttcg ttcaagaggg
    21001 cgttcatgat caatatctgt ccgaccatct catcgacttc gagattgcgc cgcttcttca
    21061 atagtccgga ggcacaaaac gagcaaccca tattgcatcc gacttgactg gagacgcaca
    21121 gagcgtttcc gtaattatag cgaaggagcg cggtctcaat tttgtggccg tcgcctaatt
    21181 ctaaaagcag cttaatcgtg ccgtcttccg acttttgggt tttatagatt ttcggaactt
    21241 caaagtagta ctcgcccttt aagacttcac gaaacgataa cgagacatcg ctcatctcat
    21301 caaaggattg ggctttcttt tcataaatcc atgaaaaaag ctgcgtggcc cgatatgatt
    21361 tctggccttt ggcaagcata gcttgaacaa gtttttcatg gctgatgccg taaatattat
    21421 tcactcggca gagcctcgac ttttcgtttc atgatcgcat aaaaaagcgc ggtgtcgagg
    21481 gggtcaaaag gaaatctctg cttttcttct aaaagcgcaa aatcacggtg ccggtcaagg
    21541 aaatccataa tcagcgagtg accttctttg cgattgatgg tcgacaccag ataaacgaga
    21601 attccgtcat cgccgacaaa agtcgcgcag ttttccagcg tttctttttg cttggcgact
    21661 aattcgtcga tgctttcgcg tttaaaatgc aacaggtaat cggggtaggc ccgaattaaa
    21721 tcaaaatcgg aactgcgagg gtggacgatg acgagatcct ggggtcttga gatggcggct
    21781 ttcatcgccg tcggctcagc gccgaaaaag ttcacgttcg tcagttggtt atagcgaata
    21841 atcttcgaaa tatcgaggcg ttcatcgaca ttaggaacgc cgagatttaa accgatggtc
    21901 ttttgatatt tgaggaagaa ttcacgaatt acggaattgt cttccgccga atacaaaaag
    21961 gcttcggtga cattgttcag gcagactttg tccatcagtt ccttgtggcc catctttaat
    22021 agaaagacat caaaactctg aaagaaagta ttcttgcgaa tgggggcttt accttggtaa
    22081 ataaccatgt cgacaagcgg ggcttcgcga aattcgggat tatcgtcaag gacttcttta
    22141 gttgaggtgg ccagcgtgtt gacccggcag ctttgcaact gcggtttgtt gttctttttc
    22201 agcagtttat acgtcacgcc gcgaccgaag tgcttctgcc acattttaac gagccattcg
    22261 ggagtgttaa agcgaagcga caagtattcg tatgattcaa cgggaatcga ttcgggaatc
    22321 agttcgcgcg gcgattcctg aaaagccaat agcttttcga tgatgatcat ctgctcttca
    22381 ttaaacgcct ggcgaacata atcaatcatc tcatcatgtt cgatgcgctt taaaaaaaga
    22441 ttgttggcca aagaggcata aaggaggcat ttctgctcat cggtgtagtc ggttccgagg
    22501 tcgcttacga tgcgctgaaa gaggtagtga tgacgaagct cgcagccgac gatggcggag
    22561 acgaggctgg aatgctcttt atcttcgggg tgatctttaa acgcctgtcg caaggcatgc
    22621 tgaaattcga catcctgata gaggatttga ttcagaacat tcttgacgat ttccaatgct
    22681 ttcatatttc gttccctaat acatagtata gtaatagaaa atggcggtta ctactataaa
    22741 aaacgacaaa atgtcgttaa aagtgaactg atttatttgt ttctgtcatc gagttcggca
    22801 aagatattgc cgtcgtaaat gtcttcttcc tcatcttcca aaggctcgtc gaccaccacc
    22861 gcattttgct cggtgataaa gcgcggatag tcgaaattaa tataggtgtg acgatgtttt
    22921 ttatcataat aaaagtattc gacgctgatg tggatggcga tgtttttaag tgtcttaaaa
    22981 aaataggcgg tcaccttgtc ttcgaggact tcgcattcct gcggagcgtg aatgcggacg
    23041 agggcattcc acgaagtctg gtcaaagccc tcgtgaaaat aaaaggcgtt gggtgaatag
    23101 aaacttatct cactcggttt gacctcatag agatcagcga gctttttggt gttttcttca
    23161 gaatatgtgc cgaccaaata ttggtcgagg ccgaggatgg agatggtgat catagtggct
    23221 tccttattgt tgtgaacgtc attattatag cactcgcata aactttttat taaaagttat
    23281 aagggtcgac attaatcgcg acgttgatct gtcctttgct ttgcaaggcg acgagcaatt
    23341 tgcgaaggtg gttttttatc tcaacatcag acttatactt cagaagcaag acgcggtgat
    23401 gcacttcctt ttcaaagtga ataaacggcg agacgggacc gagaatcgtg acatcgggat
    23461 aattgctttt ttgaacatcc tcggcaatcg cgtgaatggt atcgatggcc agggcttcgt
    23521 tttttgaaga aatctggacg ctggtcaaaa aagtataagg gggatattgc tgggctctgc
    23581 gagccaacat ttctttttta aagaaggttt cataatcctg agcggccgcc aggcgaatcg
    23641 catagtgggt aggattataa gtctgaatga tcgcctcgcc gacttttgcg gctctcccac
    23701 ttcgtccgac ggcttgagtg attaattcga aggcgcgttc gctgctgcga aatgagggga
    23761 gcgataagcc gatgtcggcc aagacgattc cgactaatgt cacatccgga aaatcgtggc
    23821 ccttggcaat catctgcgtg ccgactaaaa tatcagcttc tttattgcga aaagcttcga
    23881 tcgtcttggc aatcgtattg cggacgcgac cgatgtcgct atccaggcgc aaggttttgg
    23941 cacgcggaaa cagtttggca atttcgcttg tgattcgctc cgttccaaag ccgcttttcg
    24001 aaaaataagt accgccgcat tcaggacaag acgtgggggc gaactcgaca tggctgcaat
    24061 gatggcactt taacatttca tcttcgcggt gatatgtcag cgtgatgcca cagtccggac
    24121 acttgaagat atgaccgcaa gagcggcagg tcacataacc gctgaaacca cgccgattaa
    24181 ttaatagaac aatctgctct tttttatcga ggcgatcgtt gattttttga atgaggagct
    24241 ccgaaaagag caccgattgg cgcgtcagac tgcgcggatt agacagatcg ataatcgtta
    24301 ctttaggcaa tacttgatcg ttgatgcgat gaggaagttc ggcgtaatgg tagacgcctt
    24361 ttaatgcccg cgctttactt tcaagggagg gggtggcgct ccccaagacg actttggcgt
    24421 ggtgatattc ggcgcgcatt atggcaattt cacgagcgtg ataaaaaggg gtggtgtcct
    24481 gtttatatga ctcgacatgc tcctcatcta aaataaagag gccgatattt tctaacgggg
    24541 cgaaaatcgc gcttctcgcg ccgacgacca cacgggtttc gcctttggcg atgcgccggt
    24601 attcatcata tttttcggcc tcggttaact cgctgtgcaa aatggcgaca tcgcctttaa
    24661 agcggcgctg gaaatactcg accataatcg gggttagcgc gatttcggga acgagcatca
    24721 agacattttt tcccgaagcg ataatcgctt ctgaaagggc gaggtatact tcggttttgc
    24781 cgctgccggt caccccttgt aataaactga cggttttgtc ggtctctaaa atgcttttaa
    24841 cggcggattt ctgctcatcg ttgaggattt ttaccgtctc tttttgatac tccggaatct
    24901 ccaatcgcga acgttcgatt tcgattattt tcaagtgttt tgtcgcgatt agcttattga
    24961 cgatgctcgg cgaaccggca tctcttttca aaacttttcc attcttttta accaatcgca
    25021 aaagatcgat ctgacgagcg gttaaacccg cctcgttatc atcgatggcg acgaggtatt
    25081 tttcatagta ggcttttggc ccgctgagcg cggagagacg gggcttgagc gacttgggga
    25141 gcatcgtctg caaaacgctt attttcgtca ccagatagtg atcgcagatg cgctcgacca
    25201 agttcatcag ttcatcattg agaagctcgc tgtcgtcgat gacatcgata atctcagaaa
    25261 gagtgaaacc tttttcggtt tcgtaatccg caagcgataa atcgctttct tcgactttta
    25321 tcacatatcc gaccagttcc cgcctgttaa aattgattaa gacccgaaag ccgacgcgga
    25381 cctccttgcg accgaaataa acataagaaa aagggcggtt aagcgcattt actcgatgtt
    25441 cggtaagtac ttctaaaata cgcatgtcgg ccctttatta tttgatgatt ttggcattga
    25501 ttttatttct gataatctct cgtatttcct ttccggcgcg atccggacga tcatttaaaa
    25561 caacatattc gtaatgatat ttaagatgca tttccttgcg ggctttttgc agtcgcttct
    25621 gaatgacgtc ttccggttcg gtcgagcgat ggcgaattct tttttcgaga tcttcatagc
    25681 tcggcggaat gacaaaaatc gagacgacat tgctgccttt gcagcggctt aagacttgtt
    25741 ttgttccttc gacttcaatt tcgaggacga catttttgcc ttgagcgcgc agctcatcga
    25801 cataatgctt aggcgttccg tagcgatttc cgacaaactc cgcccattca agaaaattgc
    25861 cggctttgat attgcgatcg aactcttcgc gcgagacgaa aaaataatcg acgccgtcaa
    25921 cttctgtttc gcgtctgctt ctcgtcgtca tcgagacgct gtaaaccaaa tttaaactgt
    25981 catcgcccat gacgttgcgg cgaatcgtgc ctttgccgac accgctcggg ccggaaataa
    26041 taataagaag accttctttt ggggttgccg tttccatata agtaaattaa ttatatgtga
    26101 agatataact taaatcaatt ctaattatgg tataattatg gcatgagaat cagccttcag
    26161 gatttttcgc aagttgtcga catcatcgct aaagaagcga ccggcaaacg cattcgcaag
    26221 gtcatctttt attcgagtcg gattttttac ctgcaaatct catcgggccg caaacgatac
    26281 ctcgtcatca atcttgataa caccaacccc tcgatttttc ttggcgaact atcgaatacg
    26341 ccgccaactc tcgtaaatcc actatatctt tttctaaaaa aagaactgaa taatgctctg
    26401 atcaagaaag tcgaacagca aaataacgac cgcgtcgtcc gcatcgaact cgagataacc
    26461 aacagcgcat acaaaacatt cacccgcaat ctctatgttg aactcatcgc cacccgaccc
    26521 aatttaatct tcgtcgatga agatgaaaaa attctcaatg ccttccgcaa gaacacgatt
    26581 gactcgcccc gcattgtggc ccgcggtttt aagtatgaaa aaccgctcaa cggcgccaag
    26641 agcaagaatg gttcagcaga accctttgat cacaccaaat ttctaaactt cgaactgagc
    26701 cgcctcaatc aatccgcgat gacgcgcaat caggaaaagt ttgtcaaaat ttataagttt
    26761 atcaatacgc gcatcaggac cgcctcgcgg aaaatcgata acattaaggc cgatgtgcaa
    26821 agagcagaag agcaacttat caacaaagaa tacggcgatg ctgtcttagc gcatcccgcc
    26881 gataaaaaat ggcggaccga caagattgaa gccaatggca ttatcattcg cctcgacggc
    26941 cttataaccc tcgccgaaaa cgcccagcac ttttataagc tatacaaaaa agccaagaac
    27001 actatcgcta taagcgataa gcttatcgaa aaagctgaga aagagttaaa tgagagcctc
    27061 ttcatcaagg attttctctc ttcgtcaacc gaagaagaaa ttgagcggtt tgtagctcag
    27121 aacgccctca tctcaccacg cgataaaatc cgccttcccg tcaccaaaaa atacgcccct
    27181 tatcatcttg aaaataacgg gacaactttt tattttggca aaaccgccga ccaaaatgat
    27241 tacctgtctt ttgcctatgc tgcccgccag catatctggt ttcacctcct caaccagccc
    27301 ggcgctcatg tcgtcatcaa aaagagcaac ccgaccgatg aagatttgat cgtggccgcc
    27361 gaaatcgccc tcctatgttc atcgcttgac cgcggcgaag tcatgtacgc cagcaaaaaa
    27421 gacatctcgc gtggcgagag caagggcgaa gctcatgtca aaaaatacga gacgatttat
    27481 atttcgttta tccgcccggc caccgaagca ctttataaag ccgccactcg cttagaggtc
    27541 aaataaatgc cttctgtctc ggaaattcga aacgtctatc atgctatagc cggtgaaaaa
    27601 gcagccaaga ttgacccctt gaaggccggt ttttccaatc actcttacct gattaacaat
    27661 caccgcgttt tgcgaatcaa gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27781 nnntatgagc gcaaaaccat cgcctcagtc cggcgttttt atgacgagga cccttttgtc
    27841 ttatgccata acgatgtcgt taacggcaat cttttatttc gcgccaacaa agtatttatc
    27901 atcgattttg aatatgcggc cgataataat cccctcttcg atctggcgag ttttttaagt
    27961 gaaaacgaca tcgaagacga aaggttgaga cgcctgttct taaaaactta tttcggaagg
    28021 cgctgcgagg agcggattta ccgccgcctc acccgcatca tcgccgtgca ggatatcctc
    28081 tggtattact gggcccagat gcactatgtt agcggcaaag agctaatcta caaacgcatc
    28141 gctcatcaca agtggaaggc gataatgaaa agcgcctcat cgcattgacc aggcgctttt
    28201 gccaatcgga ttattttaat aggcgaacgg ttcgtactcg acgaatttat ccacgtatgc
    28261 tttcagactt ttggcgataa cctcgcgggc gtgtttggcc cccttcatgt tattgacgat
    28321 cgtctttttg ttttcgagtt gtttgtaaac gcggaagaag tggcgaattt cattggagat
    28381 atgaggtggc aactcgcgaa tatctttata gatattatag attggatcgt tcttgcacac
    28441 agcgatgatt ttctcatcga ggtatccgcc gtcgatcatc tcgaggacgc cgatcggata
    28501 gcaatctacc aaagccagcg gcacgattga ttccgaggac aaaacaagaa cgtccaaggg
    28561 gtcgagatct tcggcgtaag taagggggat aaaaccataa ttctgcggat agtgagtcga
    28621 ggtgtaaagg atgcggtcga gaatcagcgc tcccgtctgt ttttcgagtt cgtatttgtt
    28681 cttgctgcct ttggatattt cgatacacgc taaaaactgc tcgggtttaa ttcgctcgcg
    28741 gggagccatg tgccatacgt tcatcttttt ttcctcgtaa attctattga tattatacca
    28801 atcgcctccc tattatacca agaaaatggc gaagcgcgag atgttcttaa aaccgccaat
    28861 cagtattctt tttttatcat tttcggagtg ttatactacg tagtatgaaa atgttcaaag
    28921 cctccttgct cgtcctgatt cccctgctct tatcatcctg cggcaatcgt ccccgcggat
    28981 tggttttaat cagccaaaac ggcagtttat atgatattca agaaccgaca gccaccagcg
    29041 agctgagcga ttatgtccgt catacggaca gtccggagat tttatcatac ttacaaaatg
    29101 acgagccgtt tgtcctctac gtcggtatgg ccggctgcct gggctgcaat atgttcaaac
    29161 ctaatctcca gcgatatgtc tatgagacgc aagccttgat tcactatctc aatgtcggcg
    29221 ataacgatga ctatctcgaa tattcaaaaa tctggaccgg ctatcaggat atatttatgg
    29281 ccgatcttga agtcccttat ctcatgatcg tcgaaagtga cgaggcgtat gtcaaaggcg
    29341 ctgtctcaaa gatgacggcg acgaccgttg agccctttat caacatgatg aattcgctcg
    29401 tcgatgtagc aaatgtcgct tcgcacattc aaaatgattc tgcaactaat tatctggctt
    29461 caagcgaaga aggcctcttc ttcttttatg accgcgatga cgaggatgct ctggaaatat
    29521 atcggacgct gatttggccg tacgccgcaa agagcgagcg ttcgctgcaa gtcgtggatt
    29581 acactaattt caccgaggag gaactcatcg ttttacgagc gactttctcg cttggcgaag
    29641 acatcggtcc catcgccaaa tactatgaag gcgaagcgat gatcgaagcc cattacttcg
    29701 gcatcgatga ggcttccgac cggcaatttc tcgaagctta tctttagata aaaaaaccgg
    29761 gtgttcccgg tttttcttat ttaaccttga ttcttttcaa gacggcagat ttatacatgc
    29821 cgttttcaaa cttgatgtcg accgagccct gaatgagggg cagacagtaa cgcctgaatg
    29881 attcatccat atggctgggg cccgccatca ttgaaggggg gacgacgcgc tcggcattag
    29941 cgattttggc gatgtcttcc ggaacgtatt cgacgcggta gggctcatcg cttaagcgct
    30001 tgatgcaaac catctcgccg gtgtgacctt gcaaggctaa ttggacggca atcgtcccgc
    30061 aggcaatcgc ctcttgtcga tcgactttgc tgaccaataa aggatcggcg cgctgcggta
    30121 aagagagctc gattgaacga gtcggaagat cgagacgaac ctcgaccatg tcggccagtt
    30181 catcgccgac gccgccgagc tgaatgtgat taaaggcatc gacgcgagct ttggtcatat
    30241 cccgttcaaa ggaaatgcct tcggagatga cgacgatcgc attgcccttt ttgtcgaaaa
    30301 gtttttttac atcgcggaga aaatcatcga cgctaaatgg attttccggt aagtagatca
    30361 aatcgggtct cgtctcttcg gggagaagat cgacggaagc ggtcagccag ccggcattgc
    30421 ggcccatcac ttcaataata taaactttgc cgcgtttata aacgcgagca tcgacgcaaa
    30481 ttgatttaac ggtattgacg acatgtttgg cggcgctggc aaaaccgagt gaatgatcgg
    30541 tcaacgccaa atcgttatcg acggtttttg gcacgcccat aaccatgcag tcatagccga
    30601 tttcgcggca tagaagcgat aatttgtggc atgtgtccat cgagtcattc ccgccgttga
    30661 caaacacata gcctatgttg tgttttttta aggtttcgat aatgctcatg tattgtttat
    30721 cgtgcaaatc cttagggagc ttataacgcg ttgagccgag ggcggcaccg ggagtctgct
    30781 taagcagttc aatcgtctca tcgtcttctt gaccaaggtc gatcaaatca tcgataatca
    30841 gaccttcgac gccgtaaagc gaaccgtata tcttatcgat tttatcaggg tatttcttgg
    30901 cttcgacaac cgctccgtat agcgaggtgt tgatgactgc tgtcggtccc ccgctctgaa
    30961 tataaaccat ggcttttttc attaagttct cctcattaac atatttctta taataacttc
    31021 gtaagacttt tccttcacaa acaagtgttt ctcgaggcag acatctcgta agaaggagat
    31081 actatctttc gagtcaacat cgcctataat tctaaggaat tattcaaata atgtaaagaa
    31141 agccaaaagc gggttatgtg agattaaagt gtaacgcgga gaaagcacat aaatccgaga
    31201 agctatgctt ctttttttca tctctgagtt attctaatag aaaacggacc cttaaacggc
    31261 taaaatgtaa gcggttgcat agtatggctt aatctagtat tatctttact acacgctcac
    31321 aagagaggta tccatatggc atttacccta aagtttcacg atcaagaaaa gagcttcgat
    31381 aagaaggtcg ccctccttga tctcgtcgat gatccccaaa aagaattcgt ctgcgcgaag
    31441 gtcaacaacc gcattcgcga actgacgtat gaagtatatt acgacgcgga tgttgagttt
    31501 ttaaccgtcc gtgacgccga cgcgattcct atttacgaga catcgcttcg ctatctagtg
    31561 gccatggctt ttaaacgcgc ttatcccgaa ctcgaagtgc gcttcagcta taatgtttcg
    31621 cgttcgatat tcgtgcaaat tctcaacgat tcacgtcata gcgatatgtc gatggtacgc
    31681 cgtatcgaag atgagatgca gacgattgtc gccgctgatt tcccacttaa tcgtatgatt
    31741 gtgaccaagg aagaagccaa gaacatctat gaagataaga agttcaccga caagttagaa
    31801 ctccttaaat atcgccccga aaagactgtt catctctacg attgtgatgg ctatctcaat
    31861 tacatgtatg ggcggatggt tccttcgacc ggctatctaa aaaaatacaa gattcgcctc
    31921 tattcgccgg gaattatcat tcaatatcct cgcgccgaag cgggtggaga tattcctaag
    31981 tttgaagacg ctccgacttt cggcaagacg ctcaaggcct cgcacgcctg gggcaaaatc
    32041 gccggcgccg attcggtttc cggaattaac gaacacatca agaaaaatgg cgttatcgat
    32101 tttatcaata tgtgcgagac gcgccacaac cgcatgctcg ttgaactcgg caacatgatt
    32161 gaaagcgata tcgacacgat tcgcctaatc tgcatcgccg gtccttcctc gagcggcaaa
    32221 acgacatttt ccaaccgcct gcgcatcgaa ctgctctcgc gcggaataaa accaattcgt
    32281 ttgagcatcg atgactatta cttgccgaaa agcgaagttc ccaaggatga aaacggcgat
    32341 cccgatttag aatcgattaa cgccctcgat atcgaactat ttaatcagaa tatgctcgac
    32401 ctcatcagcg gcaaggaagt ccagcttccc cgctttgatt tcaagctcgg tcaccgcgtt
    32461 ccaggtcgca aactaaaagt atcttttagc cagcccatca tcatcgaggg aattcatgcc
    32521 cttaatgata tgctgaccat caacatcccc aagcatcaaa aattcaaaat ttatatctcg
    32581 cctcaggccc agattaactt cgataatcac aatccgatgt ccttgaccga tcttcggctt
    32641 ctccgccgca tcgtccgcga caaaaaatac cgcaacgctt cagctgaaga gacgatgagc
    32701 atgtggccca gtgtccgcaa aggcgaattc acctggattt acaacacgca ggaaggcgct
    32761 gattttgtct ttaattccct ccttagttat gagctctctg tcatgaaaaa atatgcgatg
    32821 ccgctactca gcaatatcga taccaactcc gtttatttcc cggttgccga acgtctgatt
    32881 cgcatgctta aatatttcga tgacatgccg gacgagtggg ttccaagcaa ctccctgctt
    32941 cgtgagttta tcggcggctc ttgctatgca aatgtcgacg tctagccttc tgacatcatc
    33001 ggccaatttt catttttatg caaatgagca gagtggataa aaccattctg cttttttggt
    33061 ataattaagg gagtcagaaa ggcatcaata atgaaagatt ttcatgagtt cgatgcgcgg
    33121 ttagaaaaga attttggcat attttcgccc gcggaattat caaagatagc taagaccaga
    33181 gtactcattg tcgggctcgg cggaatcggc ggctacctcg cgaatgccct cgcccgcctt
    33241 ggtgttcgcc agctcaccct cgtcgatttc gacaagttca aggtgacgaa tttaaaccgc
    33301 cagctatttt cttctttaaa gaccatcaac aagttcaaag ttgatatcat caaagatgaa
    33361 ttattaaaaa ttgacccaac actcaaaata gatagccaac cgctttcaat cgaggcgctc
    33421 gatgaagatg tcgtcagcaa agttgatgtc gtttttgatg ccgtcgatgt catcaagacc
    33481 aaaatttatc tcgagaagat gtgttcaaaa tacaatctgc ctctaattca cggggcattg
    33541 ggtggctggt ttggtcaagt cggcatctca ctaccgaaat catcgcttct cgaaaatttc
    33601 tacggtcagg acgtctcggg gcttgaagag actatgggcg ctccgacctt tatcccgcct
    33661 ctcgtagccc attacatggt cgttcagttt atccttttat gcctcggcaa aaaagaggct
    33721 ttaataaatc aaattctcaa catcgatgct cttcataccg aactatctgt cgtctataag
    33781 aaatagggtg cgatatggta aaaatcattt tcttcaatct cttgcgcagt aagtatcatt
    33841 ggcacgaact cgacgtcgcg ccgggaactt tccaaaacgt catcgacgaa ttaaaaaaga
    33901 acaatccgtc tctggatgaa aaggatttcc gcgactgcgt catgtttgtc aatgatgtcc
    33961 gcatcactca tctagcgcga tttgacatcc aatttaaaga tggtgataag gttgtcttaa
    34021 cccattttct cggcggcggt tagttttttg tttattgctt cttactatgc ccggccatct
    34081 tgcgcatctt cgctcgctcc tgagcgtgaa ggctcacgag ctgaccgtag tatatcttgc
    34141 gcatcaggag aaatctttgc ttttcggatt cgctgatcga gcgcacataa atctccgcaa
    34201 aaatcttttc gaggcgaaaa aaagattcgt agacattgac aagggcaaga tgcatgaaat
    34261 actcgcgcct tgaataaatc agaagcggcg aactgtgagc aatttcgctc gacatttcat
    34321 agatcggtgc ataacgtttt aatcccgccg tcttctctaa gccgtcgcgg aagttgagtt
    34381 tgacgggttc ggccttgtca agggcagata ccttcaggag ccaaccatat tcaatgaagc
    34441 gcttcatatc tttgcttttt aagtcaaaag tccgcatttc tcctttaatt tgtgtgaaaa
    34501 ttgcatccgt tttttcttta tctcccgcta aaccgcgaaa agccatagcg tagttcatat
    34561 gcaataaata cttgtcgata accggctttc cgtacttcgt caaaacaagc aagatgcact
    34621 ccgcttcatg aagggttcgc caagtagaaa aagcctcggt ttcaaatccg gagacgagga
    34681 gatcgctgat cgcgcgagcc atcgaaaaac cctttgacat aatatcgaca attaaggttt
    34741 cattcggctg attgcggcta tatcggccca ggacgccgag gacgaaattg agatacgtat
    34801 taagcgttga tatgcccggc gaaaagcgcg acaagatcgt ctttgattta taggagaggt
    34861 gctcgttagt gtagtactta tcaagggcga tgctgacgat catctgcata tatttttcgt
    34921 ctttgatcac cgcttcttgg gcagcatcgg ggtgaatgtc gagatagtag ccgtgctcat
    34981 taacaatata aagaatcagg gcgatgggat tgacgggcac gacacggccg agcaggttgg
    35041 cttcatcgat gctcttcgcg ccttccatag cattttgata aacatcatat aaaaattgag
    35101 agtcgaggct ttctttaacg ccgagagctt tatagatatc ttgaaactgt tcgaaggaga
    35161 tgacttcttg tttactcatg tttaatcctt taatttgaac atcatcgtcg cctgaacagc
    35221 ctgtttccag ccctcgtatt tcttgatggc aactttttca tccatcttgg gctggtaagt
    35281 ggcctgtagg caatgatact tttcaatttc cttcatattc ttccaaaagc cgctcccgag
    35341 gccagccata taggcgacgc cgagcgatgt tgtttcaagg cattgcggaa ggcgaatttc
    35401 acagcggaga atatcggcct gaaactggag taacaactta tttttcgtcg ctccgccatc
    35461 gactcgcaaa gccgatacat cggcgtgggc ctcctgcttc atgacttcga tgacatcttt
    35521 tgactgatag gcaatcgctt ctaaagaagc gcgcacaaag tgcgatttat tcgtgccgcg
    35581 cgtgatgcca aagaccgccc cgcgggcttc attatcccaa taaggtgttc ctaatccgac
    35641 aaaagccggg acgatataaa cgccctgcga gtcgtcgacc ttgcgggcaa ctctttcgct
    35701 ttcgtcgctt gttgtgatga tattcatcga ttcgcgaagc cactggacga ccgcaccgcc
    35761 gacaaagacg cttccttcta acgcataatt tacttcatcg ccgatctgcc aggcgacggt
    35821 cgtcaataat ccgttttgcg aaagaacggc ttctttacca gtattcatca acgcgaaaca
    35881 gccggttccg tatgtgtttt tcaaatcgcc agcaataaag catgcctggc cgaagagggc
    35941 ggcttgctga tcgccgcaaa ccccgtagat gtgaatattt ttagaaaaga aactggctat
    36001 gccgtaatcg gcgcttgatg gcttgacttc gggtaagaga gatttgggga tattgaataa
    36061 ggtgagcaac tcgtcatccc acttcatgtc aaaaatatta aaaaacatcg tccgagaagc
    36121 attgctcggg tcggtggcgt ggactttgcc tttagtcatg ttgtaaatga tccaggtatc
    36181 gaccgtgcca aacatcagtt ctccagcttc cgcgcgcttt tgggcgccgg caactttgtc
    36241 gaggatatag cgaactttcg aggcgctgaa atacggattg atcagcagac cggttttttt
    36301 atgaattaag tcacgatatt tggcaatttt ctgacaatag ggcagcgatt ggcgcgattg
    36361 ccagacgatg gcgttataaa tcggccgccc cgttttgcgg tcccaaataa ttgtcgtctc
    36421 gcgctgattg gtgatgccga tcgcggcgat atcgtccatc gttttatgag aaattaccaa
    36481 tagttcattg acgacgtcga tgacgctgac ccaaagttgg agggcatctt gttcaaccca
    36541 tccgggttta ggatata
//
LOCUS       DAOSSL010000005        34809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1368_length_34809_cov_18.491253, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000005 DAOSSL010000000
VERSION     DAOSSL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 34809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34809
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_34809_cov_18.491253"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..710
                     /locus_tag="PLH17_01145"
     CDS             123..710
                     /locus_tag="PLH17_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20420.1"
                     /translation="MEKRVIKRYEYELVQLLKQSEEVSIFLAQRKDRFFTFREYRLED
                     AYNEDLAKYKRIQKIGVNVQKLIAHDRQLLVLIFERFQEPTALDVLAKEELKEIYFKQ
                     LFNIYRFCRFSKVELNYMPNNYILKGTTLYYVGEQITDSNASRNLENFGLRYWIYSKE
                     ATELLEKKGHPIDAKRILSEEEANKRIVLLSVTYW"
     gene            723..1679
                     /locus_tag="PLH17_01150"
     CDS             723..1679
                     /locus_tag="PLH17_01150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOR20421.1"
                     /translation="MLEKIILFNLSSNPDLTKKIAGHLKLPIGQVELMHFADGEIMAR
                     SLDSVRGKSVYIIQSTCSPASERLMELLVFIDGIKKANAKDINVVIPYYGYSRQDRIA
                     RPREPISARLVADLLQTVGVSHLITIDLHTPQIQGFFSCPVDNLSTIHLFAAYYLKKL
                     KQIKVDTSEIVVVSPDHGSAHRGRDLATALPGSQLAIVDKRRPSPNEAEVVNIVGDVE
                     GKTCIIIDDIIDTGGTIIASAKELYAKGANDILIAASHGVFSRDNIDSLKATGISDIV
                     ITDSIEKNIQGVNVISVSEVLAQVITHNESGTSLPPEYLAFY"
     gene            1723..2241
                     /locus_tag="PLH17_01155"
     CDS             1723..2241
                     /locus_tag="PLH17_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOR20422.1"
                     /translation="MNKENRNRSIIERISINAMIASIYFILTMATLPVSFMGMQIRIA
                     EMLILLCFFRKDFVIGISVGTLLANTMSPMFAWDMLFGTLATLISGLLIGYMKQLFVA
                     AIIPVVINAFVVGLELHLIMGLPFWLNVGLVGLGEFIAVAILGYLILLFLGKNEKFQE
                     MIGANQNYRFKW"
     gene            2326..3450
                     /gene="tgt"
                     /locus_tag="PLH17_01160"
     CDS             2326..3450
                     /gene="tgt"
                     /locus_tag="PLH17_01160"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOR20423.1"
                     /translation="MENFFLEIKHVCRQTGARYGILHTPHGDVEVPMFMPVGTLATVK
                     TLSPEEIKESGAGVILANTYHLHLRPGEDIVEKAGGIHKFMNYNGPILTDSGGFQVFS
                     LMENRKINEEGVIFKNHLNGSRIEFTPEKVIAIQEKIGADIIMSFDECMPYPVEHKYA
                     QASVERTLRWAKRGLEAHIRKDQALFGIVQGSDFEDLRRLSATELAKMDFPGYSIGGT
                     SIGEPKDVFIKMVEQSVKYLPHDKPRYLMGVGSIDFLLEGIERGVDMFDCVLPTRIAR
                     HGALMTSNGRVNIRDAKYKEDFSPLDANCDCYTCRNYTRAYLRHLYVSEEIFGKRLLS
                     IHNVRFLISLMEKARDAIKEDRFGDFKAEVYRSFGGSKGF"
     gene            3450..4499
                     /gene="queA"
                     /locus_tag="PLH17_01165"
     CDS             3450..4499
                     /gene="queA"
                     /locus_tag="PLH17_01165"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOR20424.1"
                     /translation="MDIKLFDFDLPEELIAQSPSLVRDKSRMISVDCSNQTYQDEHFF
                     DIIKYLKPGDVLVRNNTKVLPARLFGTKEGTGAHVEVLLLREIEKDTWECLTGNAKAI
                     KKGTVVVFKEGVLRATCSEVKDEGIRYMKFDYPGVFLEALDEVGLMPLPPYIKKQCED
                     NSRYQTVYAERLGSSAAPTAGFHFTPDLLSKIQQIGVEIVDITLHIGLGTFRPVKVQD
                     SKDHIMHSEYFEVSEEASGALNKAKSEGRRIIAVGTTSTRTLEAVYQKYGHFVACQEA
                     TDIFISPGYKFVAIDALITNFHLPRSTLIMLVSAFIGREFTMQIYAHAIKERYRFFSF
                     GDAMFIYGKQKELLS"
     gene            4471..5100
                     /locus_tag="PLH17_01170"
     CDS             4471..5100
                     /locus_tag="PLH17_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOR20425.1"
                     /translation="MENKKNYYLESLKEIEKLDKSGPKPTLLLHACCAPCSTFTLLFL
                     CPHFDITIFYNNSNIYPLSEFEKRRDELKNFLKDFKKKEGHKVALIIPEYNNDQYNIE
                     LEPYADLPEGSKRCFICYEKRMDEAYAYAEQNGFAYFATAMTISRQKNSQILNAIGEK
                     LSTRYPKTKYFHSDFKKNKGIDIAKAMRDEYGLYQQTYCGCKYTFLKKK"
     gene            5377..5688
                     /gene="rpsJ"
                     /locus_tag="PLH17_01175"
     CDS             5377..5688
                     /gene="rpsJ"
                     /locus_tag="PLH17_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012369008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOR20426.1"
                     /translation="MAKTKVIRVRLKAYDHKILDLSVEKILEAANKTGAGVVGPIPLP
                     TEKQVFTILRAVHKYKDAREQFEIRTHKRLIDITNPTKETVDTLRRLDLPSGVDIDIK
                     L"
     gene            5703..6464
                     /gene="rplC"
                     /locus_tag="PLH17_01180"
     CDS             5703..6464
                     /gene="rplC"
                     /locus_tag="PLH17_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOR20427.1"
                     /translation="MKSIIGRKMGMTQVFAEDGTMYPVTVIEVLPNVVTQLKTADKEG
                     YTAVQVGYEDERESRANKCEKGHFAKANVTPKKHLAELHGDELAGYKVGDAITADLFH
                     AGEIVDVIGVSKGRGFLGSIQRYGHKIGPKGHGSGYHRGVGSMALNGRPNNRVLPGKK
                     MAGHHGNQSATILNLLVIKVDAEKNYILVKGSVPGAKKSLVTIRSAVKEQLGEKQIVK
                     DLINRTPAVNAVKEEVAPAAPEVKEAPAVSEAKGE"
     gene            6467..7105
                     /gene="rplD"
                     /locus_tag="PLH17_01185"
     CDS             6467..7105
                     /gene="rplD"
                     /locus_tag="PLH17_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010580234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOR20428.1"
                     /translation="MAENKKVSVPVYNQLGEKVSTVSLKGEVFNVEVNNSAMFDAVNV
                     YRSNMRQATSKTKKRDEVSGGGKKPWRQKGTGRARAGSSRSPIWVGGGIVFGPTGDQN
                     YTLQQNKKEHRLALKSALTLKVKDGLKVVDNLELKEIKTKSFVEIVKALKAEGRILVV
                     LTDVEENLALSARNLTNVTITVPTNVSVYDLLNNQSVIMSKAAVKALEEVLI"
     gene            7105..7452
                     /gene="rplW"
                     /locus_tag="PLH17_01190"
     CDS             7105..7452
                     /gene="rplW"
                     /locus_tag="PLH17_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005376990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOR20429.1"
                     /translation="MAAKKKVEAKKVEKRFAKATLQDFDTILRPVITEKTMALMQEKN
                     QATFRVAPNANRTQVKLAFERVFGVKATEVRILNVLPKSTRRGGRYEGKISGYKKAIV
                     TVATGEALDLFKE"
     gene            7482..8315
                     /gene="rplB"
                     /locus_tag="PLH17_01195"
     CDS             7482..8315
                     /gene="rplB"
                     /locus_tag="PLH17_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOR20430.1"
                     /translation="MSIRSYKPTTPSRRNMTNSTFEEITKSTPEKSLLSKLTKTGGRN
                     NQGVITTRHIGGGHKRKYRQIDFKRNKDDLVAIVKAVEYDPNRNANICLVSYLDGEKR
                     YILAPKDLKVGDRIISSKVADIQIGNAMELGNIPEGTVVHNVELSPGKGGQLCRAAGT
                     SAQVLGKEDKYVIVRLASGEMRKILQTCRATIGSVGNEDYSLINKGKAGKTRWQGTRP
                     TVRGSVMNPVDHPHGGGEGKSPVGRDAPRTPWGKRALGVKTRKTKKASTRLIVRRRNA
                     K"
     gene            8337..8630
                     /gene="rpsS"
                     /locus_tag="PLH17_01200"
     CDS             8337..8630
                     /gene="rpsS"
                     /locus_tag="PLH17_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOR20431.1"
                     /translation="MARSLKKGPFCDAHLMNKVRKLNAANRKEIIKTWSRRSTIFPEF
                     IEHTFAVYNGREHITVYVTEDMVGHKLGEFAPTRTYRGHTGHTAEDKAATAAK"
     gene            8640..9062
                     /gene="rplV"
                     /locus_tag="PLH17_01205"
     CDS             8640..9062
                     /gene="rplV"
                     /locus_tag="PLH17_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOR20432.1"
                     /translation="MAEKKPVTRKKAVKPAKVEEKSAAPAKPKVTEARAVAYDVRLTP
                     RKARMVIDLVRGKDLNVALGILANVNRSASPAVSKVIKSAAANAINNFAMEESKLYIH
                     TIYANDGLKMKRFMPRAKGSSSGIIKRTCHIYVTIKSR"
     gene            9072..9746
                     /gene="rpsC"
                     /locus_tag="PLH17_01210"
     CDS             9072..9746
                     /gene="rpsC"
                     /locus_tag="PLH17_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOR20433.1"
                     /translation="MGQKVNPVGLRVGINKDWNSRWFADRKEYPAFLKEDMVIRQYLE
                     KNLKAALLSHIEIERAKTDKGHTVNIFCFVARPGVVIGQDGANINRIKKELGKKIATA
                     NVRIDIVEVKNPDLDAHLVAQWIASELENRASFRTAQKKAIQRVRKAGGKGIKTEVSG
                     RLGGNDIARSEGYKEGIIPLHTLRSDIDYAHTEATTTYGRLGVKVWICRGEILPEEKK
                     TKTEGE"
     gene            9752..10171
                     /gene="rplP"
                     /locus_tag="PLH17_01215"
     CDS             9752..10171
                     /gene="rplP"
                     /locus_tag="PLH17_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOR20434.1"
                     /translation="MLQPKRVKYRRPHGISYEGKSKAGNEIAFGEYGLQAVTGGWIND
                     RQIEAARIVLSRYTQRAGKVWIRIFPHLAKTKKPAEVRMGSGKGNPESWVAVVRTNRI
                     MFEIGGVDEKDAREALRLAAYKLPIKCKVVAKEAKGE"
     gene            10174..10371
                     /gene="rpmC"
                     /locus_tag="PLH17_01220"
     CDS             10174..10371
                     /gene="rpmC"
                     /locus_tag="PLH17_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOR20435.1"
                     /translation="MKISELREMSNEELNEKVYLLKEQLFNLRRKKAVGQLEHGEEIR
                     RVRKDIAKAYTIQRERELGIK"
     gene            10386..10640
                     /gene="rpsQ"
                     /locus_tag="PLH17_01225"
                     /pseudo
     CDS             10386..10640
                     /gene="rpsQ"
                     /locus_tag="PLH17_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            10767..11141
                     /gene="rplN"
                     /locus_tag="PLH17_01230"
     CDS             10767..11141
                     /gene="rplN"
                     /locus_tag="PLH17_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOR20436.1"
                     /translation="MVQNETNLVVADNSGAKSVRIFRLFGGGNRKSSSIGDIVMCAVK
                     TATPNGKVKKGEIVRGVIVRTKYPINRPDGSTINFDDNAIVLINEDGAPLGTRVFGPV
                     ARELRDKGEGFMKIVSLATEVL"
     gene            11153..11461
                     /gene="rplX"
                     /locus_tag="PLH17_01235"
     CDS             11153..11461
                     /gene="rplX"
                     /locus_tag="PLH17_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOR20437.1"
                     /translation="MRIKKGDKVIVITGGDKGKEGIVQAVFPKTHKVVVEGVNVHKKH
                     RKPTQKTPEGSIVEIYVPIDVSNVALIDPKTKKATRVTYKVEKDQKVRIAKASGSKLD
                     "
     gene            11705..12286
                     /gene="rplE"
                     /locus_tag="PLH17_01240"
     CDS             11705..12286
                     /gene="rplE"
                     /locus_tag="PLH17_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002816024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOR20438.1"
                     /translation="MKKEAPKAEPKVKVTNRLKVKYEQVVLPKLMAKYNFKSVMESPR
                     LDKIVINVGVGDAIVNAKVLDDSVRELREIAGQQPVITKAKKSIANFKLREGQAIGCK
                     VTLRGTRMYDFLDKLVSIALPRVRDFRGVSRNAFDGHGNYTLGVKEQLIFPEIDYDKV
                     NKIRGMDIVIVTTARNDDQAFTLLSELGMPFRK"
     gene            12298..12483
                     /locus_tag="PLH17_01245"
     CDS             12298..12483
                     /locus_tag="PLH17_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOR20439.1"
                     /translation="MAKTSQKVRWARPKKFKVREYTRCVRCGRPHAVYSKFHLCRICL
                     RELAYKGEIPGLKKSSW"
     gene            12498..12890
                     /gene="rpsH"
                     /locus_tag="PLH17_01250"
     CDS             12498..12890
                     /gene="rpsH"
                     /locus_tag="PLH17_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOR20440.1"
                     /translation="MMTDPIAAMLTTIRNANALRYESVTVPSSKMKVEIAKILLEEGF
                     ILDYKVLGDKKKDLSLTLKYAANKERVISGLKRISKPGLRVTVGCENLPRVLRGLGVA
                     IISTSKGVMSDKKARTLGIGGEVVAYVW"
     gene            12896..13441
                     /gene="rplF"
                     /locus_tag="PLH17_01255"
     CDS             12896..13441
                     /gene="rplF"
                     /locus_tag="PLH17_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOR20441.1"
                     /translation="MSRIGYKVIDLPSTVTVTVNDGVALVKGPKGTLEVKVPASIDLK
                     IEGALIHVSRENEERQTKENHGTFRALLHNAIVGVSEGFSKTLEIVGIGYRAALKGHD
                     LLLNIGFAHDVIVTPEPGVKITCKTANIVIVEGIDRQAVGQTAARIREVRKPEPYGGK
                     GIKYAGEHIIRKEGKRASAKK"
     gene            13455..13811
                     /gene="rplR"
                     /locus_tag="PLH17_01260"
     CDS             13455..13811
                     /gene="rplR"
                     /locus_tag="PLH17_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOR20442.1"
                     /translation="MIKKLSKNVVRQRRHARVREKISGTAEMPRLCVYRSNANIEAQI
                     IDDVKGVTLAHASSSALKLANGGNVEAAAKVGEAIAKVALEKNIKTIVFDRGGYAYHG
                     RVKALAEAARKAGLQF"
     gene            13973..14443
                     /gene="rpsE"
                     /locus_tag="PLH17_01265"
     CDS             13973..14443
                     /gene="rpsE"
                     /locus_tag="PLH17_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOR20443.1"
                     /translation="MRDEPKEYEERVVSVNRVSKTVKGGRKMRFSVLVVIGNGKGKYG
                     FAIGKAAEVPDAIKKALEKARSNTFTFKTSKGDTIAHEIIGKFGACSVFLKPATEGTG
                     IIAGGPVRAILELAGIRNVYSKVYGSRAPINIIRATHHGLSNLKSYAKVKELRK"
     gene            14459..14899
                     /gene="rplO"
                     /locus_tag="PLH17_01270"
     CDS             14459..14899
                     /gene="rplO"
                     /locus_tag="PLH17_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOR20444.1"
                     /translation="MKLHELEHAEGARTAHYRKGRGIGSGNGKTSGKGHKGQGARTGG
                     KKAPGFEGGQNPIYRRLPKFGFQNINRVEFASVNIERLNAFEDGTVVTSALLVEKGLV
                     RKEYDGVKILGGGKLTKKLTVQAHKFSKSAKEAIESAGGTVEVI"
     gene            14901..16268
                     /gene="secY"
                     /locus_tag="PLH17_01275"
     CDS             14901..16268
                     /gene="secY"
                     /locus_tag="PLH17_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOR20445.1"
                     /translation="MKKLVSVFKNKEIVSRIFFTIMILFVIRIGAAITVPGVTVSEEL
                     SKALETANMISMMDMLGGGALSNFSIFALGVSPYITAGIIIQLLSKDVLPALTELTKQ
                     GQYGRKKSEMATRYLTLLLGAVQAYGIIKTMENSQYITINMELNFWAYAYIVTIILAG
                     AMLTMWLGDQITSKGLGNGISVIIFAGIVRNLPIQIATAYRKWVEQNMGHGTNELVKG
                     SFQFGIYILAFVLIIAFVVFIELSKRKIPVQHSGKSGGQGQSMSKASFLPIKLNSSGV
                     IPVIFASSILMAPSVIASFVSTDTNAAWLKIFSYSEMTEMPGINDTTWGMPWGLLLYL
                     ILIVVFCFFYANMQIAPETLADNFRKNGSYIPGIRPGIETERYVKKVVNRVTVMGAIA
                     LAFIAALPVVLTLSGIFGESSSLAIGGTGLIIVAGVAIEINNQIDGLLAGKSFEEASQ
                     GARRR"
     gene            16265..16918
                     /locus_tag="PLH17_01280"
     CDS             16265..16918
                     /locus_tag="PLH17_01280"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOR20446.1"
                     /translation="MNIILMGPPGAGKGTQAKRLIAKYDIPHISTGDMFREAMKQGTA
                     LGLLAKRYIDQGHLVPDDVTIGIVRERLAKQDCQKGYLLDGFPRTIPQAEALEILTKE
                     IGRELEFVINIDADEKELVRRICGRRVCKVCGTPYHVDTMKPKVDGVCDVCGGPLIQR
                     ADDNEEALKVRLGHYYEQTKPLLDFYAKRGLLTSFDGTKDLNVLLQEISSLIEAKTR"
     gene            16915..17688
                     /gene="map"
                     /locus_tag="PLH17_01285"
     CDS             16915..17688
                     /gene="map"
                     /locus_tag="PLH17_01285"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOR20447.1"
                     /translation="MIIVKSPREIALMREAGQVVAKVFEKLATIIRPGISTQTIADEA
                     EKIIRSYHASPTFLGYNGFKGAICVSVNDTLVHGIPNKHHLLKEGDIVSCDVGATLKG
                     YVGDACRTYPVGKISKEAARLLRVTEESFFKAVALIKPGIHLGDISHAIQTHNEENGY
                     SLPREYTGHGIGQALHEDPYIPNIGEPHTGPVLKEGMCLAIEPMVAQGQPEVIVSNDG
                     WTVKMKDGLLSSHYENTVAVTSTGYEILTMLKQGGNENG"
     gene            17681..17926
                     /gene="infA"
                     /locus_tag="PLH17_01290"
     CDS             17681..17926
                     /gene="infA"
                     /locus_tag="PLH17_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOR20448.1"
                     /translation="MAKTDVIEVEATVVEALPNAQFKVKLENGVTIHAHVSGKIRMHY
                     IRILPGDVVTVEISPYDLTRGRITFRYKNPSSSFTKK"
     gene            17943..18056
                     /gene="rpmJ"
                     /locus_tag="PLH17_01295"
     CDS             17943..18056
                     /gene="rpmJ"
                     /locus_tag="PLH17_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOR20449.1"
                     /translation="MKVAPSVKPICDKCKVIKRKGKVMVICENPKHKQRQG"
     gene            18075..18449
                     /gene="rpsM"
                     /locus_tag="PLH17_01300"
     CDS             18075..18449
                     /gene="rpsM"
                     /locus_tag="PLH17_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOR20450.1"
                     /translation="MARIVGVDIPNEKRVVIALTYIFGIGTTKSKKILAACNVSEDIR
                     VKNLSEEQLTLIRAEVAKHRVEGDLRREVALNIKRLTEIGCYRGIRHRKGLPVRGQRT
                     RTNARTRKGKRKTIANKKVAAK"
     gene            18465..18860
                     /gene="rpsK"
                     /locus_tag="PLH17_01305"
     CDS             18465..18860
                     /gene="rpsK"
                     /locus_tag="PLH17_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOR20451.1"
                     /translation="MAKAKKTTTRKKKIKRSFTKGVAHVHSTFNNTIVTITDELGNAI
                     AWSSAGALGFKGAKKSTPFAAGLAGEAAAKIAFEQGLRSVDVNVKGPGPGRESAIRSL
                     QAAGLAIQTITDTTPIPHNGCRPPKRPRG"
     gene            18909..19853
                     /locus_tag="PLH17_01310"
     CDS             18909..19853
                     /locus_tag="PLH17_01310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOR20452.1"
                     /translation="MTFKTAIVDSTSNYGKFVIEPLERGFGLTVGNALRRVLLSALPG
                     ASVYAVEIEGARHEFSALPGIEEDVTAIILNLKDLVLKIDDDDEKSKKIEVDIKGPGA
                     LKAGDLVLPAFVSVVNPDLELAHLTDEGHLKMTMFARNGRGYVTSEGNKLARTHQQVG
                     VIATDSNYSPITKASYVVEPTRVGHDSNFDRLTLEVWTNGSMTPQDAVALSSRILIGH
                     FEEFLKLQELTRDINIIKEVVETPVDDVGDKAIEELDLSVRSYNCLKRAGIQTIRELI
                     QKSEEEMMKVRNLGKKSLKEIKDKLVEMGLSFRDYIAE"
     gene            19862..20266
                     /gene="rplQ"
                     /locus_tag="PLH17_01315"
     CDS             19862..20266
                     /gene="rplQ"
                     /locus_tag="PLH17_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOR20453.1"
                     /translation="MAAQGRKNVHGKTGVRFKAAYTASKRKNMLRNVVTELIVHEKVV
                     VTSGVVKELKKLADHLISLAKRGDLHARRQAASVLRKVVIDEKESLTALDKLFDDLGP
                     AFKARNGGYTRALKADNRRGDNAKRVLVTWVR"
     gene            20389..21618
                     /locus_tag="PLH17_01320"
     CDS             20389..21618
                     /locus_tag="PLH17_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019251313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR20454.1"
                     /translation="MSKKYLQELEPGYTPLTVCEEASRCLLCLDAPCSKDCPAGTDPG
                     RFIRAVRFRNFKGAAEVVRENNVLGSICARVCPTERYCQKGCARSGIDVPIDIGRIQR
                     FITDFEETNGMKILKKGKPNGKRVAIIGSGPAALQAAASLARLGYEINLYEKEAKAGG
                     YLRYGIPEYRLPTHIVDKEIEYVTELGVKIHLNTTVGKDISLDKLKSDNDAVIVAVGA
                     SYGKMLPKFENNPHVEIAVDFLKRVKDSKGQVDLPNNILVIGGGDVAMDVNVTLKLLG
                     VKNISDVVYEEFCEFRASKKELRDAQALGVSIIDGYVPVDVDGKTVTFKHRTIPGEVK
                     ITADKIILAVGQYPNVCGLEGIRIVNGESDIKGYVTTDPKVFVAGDIAHLDKTVVAAV
                     RTGKDVASLIHSRIGGR"
     gene            21620..22873
                     /gene="preA"
                     /locus_tag="PLH17_01325"
     CDS             21620..22873
                     /gene="preA"
                     /locus_tag="PLH17_01325"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007124513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HOR20455.1"
                     /translation="MIKKDLSINFLGVRFPNPFCLSSSPVGNCYDMCKKAYGAGWGGV
                     VFKTMGYYNFVVDEVSPRFDKLNKENTPFVGFKNMEQIAEHPLDENLADIRRLKKEFP
                     YQVLIASIMGSNDKEWEELARLAEEAGADMLEMNLSCPQMTSHAMGSDVGTNPDLCRR
                     YCQAVKRGSKLPVLAKMTPNITDMIPVAIACLEGGADGIAAINTVKSICNIDLDRKVG
                     MPIINGKSSISGYSGKAVKPIALRFIQQMRADERLKNVAISGIGGIETWEDAVEFILV
                     GSSTLQITTAIMQYGYRIIDDLRNGLMHYMDEQGVDHLSDLVGLANDNLIPAEELDRD
                     YKIYPEINVDECIKCGRCYISCYDGAHQAIEWDADKRLPSVNKEKCVGCLLCYHVCPS
                     GAIKMGEIAFKPGRQGQRKAEDIIH"
     gene            23028..23510
                     /locus_tag="PLH17_01330"
     CDS             23028..23510
                     /locus_tag="PLH17_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOR20456.1"
                     /translation="MAVIQEHAKKRIKEIVASHKDEPTPLLVVLSQIQQEFGYIPLEV
                     QEVVSEEMNIPVAEIYGVVTFYSFFSLKPKGKYIIGVCLGTACYVKGSQNIIDKFCEL
                     LKIRPGETTPDGLFTVDALRCIGACAIAPAVTINGKVYANLTVDRVEKIIKEYEGGAN
                     "
     gene            23510..25381
                     /gene="nuoF"
                     /locus_tag="PLH17_01335"
     CDS             23510..25381
                     /gene="nuoF"
                     /locus_tag="PLH17_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOR20457.1"
                     /translation="MRINTPQELASKIRACEKCLAQKFSGETGTKVLLVCGGLGCIST
                     GAGDIISLLKTKIKDRGLEDKVSVDSVGCFGFCSQGPFIRVYPGETLYRMVKPEDVDR
                     IIEEDVIGGKIIEDLIYIDPKTNQPARTEKDIDFFKKQIKIALYGSGIINPESLDEAL
                     GYGSFRAFHQVITTMKSQEVIDEILKSGLRGRGGGGFPTGRKWQFALDQKSDVKYVVC
                     NGDEGDPGAFMDRSILEGNPFAVIEGMMIAGYAIQATAGYFYVRAEYPLAVSRLKTAI
                     ALLEEAGLIGDNILNTGFNFRVYIRLGAGAFVCGEETALLNSIEGQRGQPRPRPPFPA
                     VKGLWQKPTIVNNVETLAVVPFVIRNGADEFAKLGTEKSKGTKVFALGGKVNNVGLVE
                     VPMGTTLRELIYEIGGGIPHGKKFKAIQTGGPSGGCLTEKDLDTPIDYDTLIARGSMM
                     GSGGAIVMDEDNCMVDVAKFYMEFICDESCGKCTPCRIGTKRMLEYLEKITEGRASLE
                     DLDELTELAHAVKENSLCGLGQTAANPVLSTMANFRDEYLAHVVDKKCPAHVCKKLME
                     YYIIPEKCKKCGKCARVCPVQCISGTLGKTPFLIDPLKCIKCGMCYSVCPFDSIIKR"
     gene            25395..27173
                     /locus_tag="PLH17_01340"
     CDS             25395..27173
                     /locus_tag="PLH17_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOR20458.1"
                     /translation="MAKLVNIKIEGKSFQVEEGLTILKAAKKCGFDIPSLCAFNDGKC
                     SLASCRVCLVEVKGARGLVASCAYPVNEGMEISISSPRAAKARRSSVELLLSNHSKNC
                     QQCSKNGQCELLHVATVTGARDNAYTGELTRTTLDRLAPGIVRDTSKCVICGRCIVRC
                     KEAQGIGILGQEFRGFKTIVSPADNRSFMHSPCIQCGQCINVCPTGALSENSEINAVD
                     KAFTTHKYVIVQTAPAVRAAIAEEFGEKIGTPGTGKMVAALHRLGFYRVFDVNFSADL
                     TIIEEATELLIRMKTGGKLPQITSCSPGWINYMKYFYPDIIPHISTCKSPHEMGGAII
                     KSYFAETHGLDPKDIFVVSIMPCTAKKYEKTRPQNAAVQNLPDVDAVLTTRELAKIIK
                     RAGINWQKLPEEQFDDDLIGEYSGAGVIFGVTGGVMEAALRTAYHELTGKEADIVAFS
                     EVRGLENIKEAEIDINGTKVRVAVSHGMKNAKPLLDQIRNGTSPYHFIEIMGCPGGCI
                     NGGGQPFVKPVFLPNEDDNILETYMKKRASVLYSEDEKQVVRQSHNNKDILRLYKNYL
                     GEPCGHKAHHLLHTSYVADRVRFPGK"
     gene            27230..27886
                     /locus_tag="PLH17_01345"
     CDS             27230..27886
                     /locus_tag="PLH17_01345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR20459.1"
                     /translation="MYKALLFDLDGTILHSISDITTALNVSLKTCGYNFSYDDDGTKK
                     LIGMGARNIIARVFQNRPHTEEDFNQLLKVFSHNYKIHQGRQATPYEGVIFLLRELKN
                     AGVKLFVASNKPDHLAKAIVARFFGENIFDAVYGHEEGKPEKPDPYLVNHLLTTYHIA
                     PNEALFIGDSDVDVATGHNSNLDVCLVTWGYGDYSNKLFKEATFVAQSISDLKKIVFA
                     "
     gene            27964..29301
                     /gene="cysS"
                     /locus_tag="PLH17_01350"
     CDS             27964..29301
                     /gene="cysS"
                     /locus_tag="PLH17_01350"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOR20460.1"
                     /translation="MEVRIFNSLTDRIETFVPLKPREVSIYVCGPTVYSDPHIGNLRP
                     VVVFDVLRRMFISLGYKVTFVSNFTDVDDKIINRAISEKKSEEEISNHYIQAFRNVAE
                     AVHSLAPDIAPRVTEYIPDIIRYIEVLIDKKAAYAVDGDVYFRVSSIANYGELSNINI
                     DDLISGARIEENSKKESPLDFVLWKKTTIGINWESRWSRGRPGWHTECCVMINSLFPD
                     GKIDIHGGGFDLKFPHHENEIAQSKAHNNNSIANYWMHNGFINFGEDKMSKSLGNVIL
                     AKDIIEKYNGNLIRLVLLGTYYRSPLTFTFEVMETAKIELEKIENSYRQLAVSLQLAN
                     FDLSEEQETDIPQFMEHLANDLNTPNALTELFAIIKKANGALRQSSLDYLALRKLFSS
                     IGAMLNILGIFVDYPRLSKEDKHMYTEYLKYKNDKNYAESDRLRQILMARRII"
     gene            29313..30056
                     /locus_tag="PLH17_01355"
     CDS             29313..30056
                     /locus_tag="PLH17_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOR20461.1"
                     /translation="MNGILSIDQQIVSWFHALFGGNGWGTIILVVASLLVTILCAGII
                     GFEREYHGHAAGLRTHLLVAIGSALIMTISLYGFPVIFASDTRDPARLAAQVVSGIGF
                     LGAGTIIQTGTDVKGLTTATTLWLAMAIGIAAGSGNFIIAIIATALAFVSLVSLRRIE
                     KLASRRNPIIRIVVPSERPILKEIHLVANHYGIAIRDLESQIVEYQNQSVLRLTMRCS
                     FVAKAQVTAFIDDLREKINPLDIKLSTES"
     gene            30093..30806
                     /gene="rlmB"
                     /locus_tag="PLH17_01360"
     CDS             30093..30806
                     /gene="rlmB"
                     /locus_tag="PLH17_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOR20462.1"
                     /translation="MSKLIYGRNPVLSSLGENRVEKVFLDLHFNDQEIIRKLSAKKIV
                     IERVPSSQLDDFANHGVHQGIIASVLDFRYHSLDEILTAAKSKRFPLIVILDSLKDPH
                     NLGAILRTCDAFGVDGVIIKKRGQVPLNATVAKVSTGAIDHVKVHEASNLSNVLSKLK
                     DEGFWIVSSDGSAQMEYHQIDAQRPLALILGSEGEGVSELLLKRSDYIVKIPMFGHVN
                     SLNVSVAAGILIASIIAKR"
     gene            30827..31450
                     /locus_tag="PLH17_01365"
     CDS             30827..31450
                     /locus_tag="PLH17_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20463.1"
                     /translation="MSHSQVSDEQLILCYRQNSKEAYDILLKRKHHDVLPLLKKYANQ
                     CKPFGVEMNDLYAVYLESFHKAILRFVFEKITFQTYFLKVLNRDLAGFFRLVSNPNIP
                     RNNCFSLDSEVGPDTTLTFHDVLADSSQKIDARSYVKVTSAYDLINGEPKNSREETIK
                     RIIILKVAGYSISEIASLTNLKPASIRRRLSGFNDGELADQLKKCLM"
     gene            31534..31683
                     /gene="rpmG"
                     /locus_tag="PLH17_01370"
     CDS             31534..31683
                     /gene="rpmG"
                     /locus_tag="PLH17_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOR20464.1"
                     /translation="MRQKIILICPICLNRNYTTTKRKDAATGRVELKKYCPHCNAYTL
                     HKESK"
     gene            31695..31895
                     /gene="secE"
                     /locus_tag="PLH17_01375"
     CDS             31695..31895
                     /gene="secE"
                     /locus_tag="PLH17_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOR20465.1"
                     /translation="MGVKKYTAEVIKEGKRVRWPKREELLPTIIVVVAIAAFAAIFLS
                     LEDWAAGSLLQTLKEAFAEWSK"
     gene            31898..32452
                     /gene="nusG"
                     /locus_tag="PLH17_01380"
     CDS             31898..32452
                     /gene="nusG"
                     /locus_tag="PLH17_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOR20466.1"
                     /translation="MGPELGKKQWYIVTAYSGHENKVAENIKTRITRMNLSDYIFKVL
                     VAEQEVNVLKDGLPTGKTKMKNLYPGYIFVEMVMTDESWFMVRNTPGVTGIAGSSGGG
                     QKPTPVTREEIEPVLKRMGIIDESMYDRYNVGDLVKVIHGPLEGTEGRILSIDKETGA
                     CRVETVFFGRPTPVDVEFSEIERI"
     gene            32556..34685
                     /locus_tag="PLH17_01385"
     CDS             32556..34685
                     /locus_tag="PLH17_01385"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOR20467.1"
                     /translation="MKKIIYTVEGLDCGNCAAKIERHLNKDETIAHASLDFASARLYV
                     TYKEEAYNLRELTDKIKEVNDDNLTISYPEKAGGEKKKVMSKKIISIIIRLAISTLLL
                     LVATFLVPHELHWLQVVIYSLSLGVIIYDITWKSIAKIIRLRNPLDENLLLTISAVGA
                     FLMGLPILGSHGEYIEGIMIIGFYQIGQIFEEIAILKSRQAITNAVDLRGDTANLLLE
                     DDIKVVHPNELKVNDKIVIKVGEIIPVDGLVIDGEGTLDTSSLTGEFIPVNIGEGEKV
                     LSGSILKTGSLTLRVERQYEDSAIAKILDLVTSSGERKSSAEKFITKFARFYTPVVMI
                     LAILVALVPLAFPSQSYTDWIYNALSVLVVSCPCAIVISVPLAFFSGIGLASKKGIII
                     KGSNYLDELSKLGFILTDKTGTLTYGYFEVKKAVPVGLSEAEFWDYLVAVESQSNHPI
                     AKAILHTHKRQKTHKLQQNYRELPGFGVETTYQNHRLLAGKPAFLKEAGIEVPGVDEI
                     GTIIYLAVDGNYAGFVVLNDTAKVDSKSMVANLKKMGIKTILLSGDKKDNAALIAGEL
                     NIDEHYGSLAPEDKLTILESYLAKNKKGVGFVGDGVNDAPSIIRSDVGFAMSGIGSDI
                     AVQNAEVIIVNDNPMKVVDAIRISKMTRSVAIFNIVFSLLVKFSIMVLIIVGVLGEMQ
                     MTIAMLADTGLTVLMIINSLLLLYRKV"
BASE COUNT         9755 a         8877 c         8132 g         8045 t
ORIGIN      
        1 gcgagaatac tatccgcgcg tctataagaa atacgctggc ttaaaaaaat tccccaaata
       61 atctcatatc ctcttctcca tctttttttg ctttagttaa agttttactt tagaatagga
      121 atatggaaaa aagagtaatc aaacgttacg aatacgaact tgtgcaactc ttaaagcaat
      181 cagaagaggt gagcattttt cttgctcagc gcaaagatcg cttttttacc tttcgcgaat
      241 atcgccttga ggacgcctat aacgaagatt tggcgaaata taagaggata caaaaaatcg
      301 gcgtcaacgt tcagaagctc atcgcccatg atcgccagct tctcgtcttg attttcgaac
      361 gctttcaaga accgaccgcc ctcgatgtct tggccaagga agaacttaaa gaaatatatt
      421 ttaaacagct tttcaatatc tatcgttttt gccgcttttc aaaagttgaa ctaaattata
      481 tgcccaacaa ttatatttta aagggaacga ctctttatta tgtcggcgag cagattacgg
      541 attcgaatgc ttctcgcaat ttagaaaact tcgggcttcg ctactggatt tattccaaag
      601 aagcgacgga gctcctcgaa aaaaaaggac atcccattga tgcaaaacgc atcctcagcg
      661 aagaagaagc caacaagaga attgtcctac taagcgtcac ctattggtga taaacttaaa
      721 ccatgctcga aaaaatcatc ctctttaatt tatcgtctaa tcccgacctg acaaaaaaaa
      781 tcgccggaca tttaaaattg ccgatcggac aggtcgaact catgcatttt gcagatggcg
      841 aaatcatggc ccgctcactg gattcggtac gcggcaagtc ggtttatatc atccaatcta
      901 cttgttcgcc ggcttccgaa cgcctgatgg agcttctggt gttcatcgac ggaatcaaaa
      961 aagccaatgc caaagatatc aacgtcgtca tcccttatta cggctactcc cgtcaagacc
     1021 gcatcgcccg gccccgcgaa ccgatttcgg cgcgcctagt cgccgattta ctgcagacgg
     1081 tcggcgtttc tcatctgatt accatcgatt tacatacgcc gcaaattcaa gggtttttct
     1141 cgtgccctgt cgataacttg tcgacaattc acctctttgc cgcctactac ctgaaaaagc
     1201 taaagcagat aaaagtcgac acgagcgaaa tcgtcgttgt ctcccccgat cacggcagcg
     1261 ctcatcgcgg acgcgatttg gcaaccgcgc ttcccggttc gcaacttgcg attgtcgata
     1321 agcgccgccc ctcgcccaat gaggcagaag tcgtcaatat cgtcggtgat gtcgaaggca
     1381 aaacctgcat tattatcgat gacataatcg acaccggggg aacgattatc gcctcggcca
     1441 aagagctcta tgcaaagggg gctaacgata ttttgatcgc tgcttctcac ggagtttttt
     1501 cgcgcgataa tatcgattca ttaaaagcga ccggcatcag cgatatcgtc atcaccgaca
     1561 gcattgaaaa gaatattcag ggagtcaatg tcatctctgt ttctgaagtt ctcgcccaag
     1621 tcattacaca caatgaaagc ggaacctcat taccacccga gtatcttgct ttttattaga
     1681 aaaaggcctt tttgcatcac tatttcagca tttgcagcgt aaatgaacaa agaaaataga
     1741 aatcgttcaa ttatcgaaag aatctccatc aacgcgatga ttgcgtcgat ttactttatt
     1801 ttgacgatgg ccaccttgcc cgtctctttt atgggcatgc agattcgcat tgccgagatg
     1861 ctcatccttc tttgtttttt tcgcaaggat ttcgtcattg gcatttcagt cgggaccctt
     1921 ttggccaaca cgatgtcacc gatgtttgcg tgggatatgc tcttcggaac gctggccact
     1981 ctcatcagcg gccttttaat cggctacatg aagcagctgt ttgtcgccgc catcatccct
     2041 gttgtcatca acgcctttgt cgtcggactt gaactacact taattatggg cttgcccttc
     2101 tggctgaatg tcggcttagt cggactcggc gaatttatcg ccgtggcaat cctgggttat
     2161 ctcatcttgc ttttccttgg caaaaacgag aagtttcaag aaatgatagg cgcgaatcaa
     2221 aattaccggt ttaaatggtg acagctcata tttgaacgtc attttttcct ttgccggcaa
     2281 ttttgatggc tttttaagaa tcaatatttt actattttgt gtgctatgga aaatttcttt
     2341 ttagaaatca agcatgtctg ccggcaaacc ggcgctcgct acggaatttt gcatacgcct
     2401 catggcgatg tcgaagtccc gatgtttatg ccggtcggca ccctcgccac cgttaaaacg
     2461 ctatctcccg aagaaattaa ggaatcagga gcgggtgtca ttctcgccaa cacctatcat
     2521 cttcacctcc gccctggcga agatatcgtc gaaaaagccg gtggcattca taaatttatg
     2581 aattataatg gtccgatcct taccgattcc ggaggttttc aagtcttctc gctcatggag
     2641 aaccgcaaga ttaatgagga gggcgtcatc ttcaaaaatc accttaacgg gtcaaggatt
     2701 gaattcacgc ctgaaaaagt aatcgcgatt caagaaaaaa taggtgccga tatcatcatg
     2761 tcttttgatg aatgcatgcc ctatccggtc gaacataaat atgctcaagc aagcgtcgaa
     2821 cggaccttgc gctgggccaa gcgcggactt gaagcccaca taagaaaaga ccaggctctc
     2881 ttcggcatcg ttcaaggcag cgattttgaa gatttgcgtc gcttgtcggc caccgaactg
     2941 gccaagatgg attttcccgg ctattcaatc ggcggaacat caatcggcga accaaaagat
     3001 gtttttatta aaatggttga gcaatcggtg aaatatctgc cgcacgacaa acctcgctac
     3061 ctgatgggtg tcggctcgat tgactttctt cttgaaggca ttgaaagagg cgtggatatg
     3121 ttcgactgcg ttcttcccac gcgcattgcc cgtcacggcg cactgatgac ctcgaatggc
     3181 cgcgtcaata ttcgcgacgc caaatataaa gaagactttt ctcccctcga cgccaattgc
     3241 gattgctaca catgcaggaa ttatacgaga gcttatcttc gccacctcta tgtcagcgaa
     3301 gagatttttg gtaagcgttt gctctcgatt cataatgtcc gttttttgat atctttgatg
     3361 gaaaaagcgc gcgatgcgat taaagaagac cggtttggcg attttaaagc cgaagtatac
     3421 cgctcctttg gaggcagcaa agggttctga tggatattaa actgttcgat ttcgacctgc
     3481 ccgaagaact gatcgcccaa tccccctccc ttgtgcgtga taaatcacga atgatcagtg
     3541 tcgattgctc caatcaaaca tatcaagatg aacatttttt cgatatcatc aaatatttaa
     3601 aacccggcga tgtcttggtc cgcaacaaca cgaaagtttt accagcgcgc ctgtttggaa
     3661 ctaaagaagg aaccggcgcc catgtcgagg ttttactcct ccgcgaaatc gaaaaagata
     3721 cgtgggagtg cttgaccgga aatgccaaag caattaagaa aggcaccgtc gtcgttttca
     3781 aagaaggcgt tttgcgcgcc acttgcagcg aagtcaaaga tgaaggcatt cggtatatga
     3841 agttcgacta tccgggcgta tttcttgaag cgcttgatga agtcggactc atgcccttgc
     3901 caccttatat caagaaacaa tgcgaagaca acagccgcta tcaaacggtc tatgccgagc
     3961 gtctcggaag tagcgcggca ccgacggccg gatttcattt cactcccgat ttgttgtcaa
     4021 aaattcagca aatcggcgtt gaaatcgttg atattacctt gcatatcggc ctcggaactt
     4081 tccgtcccgt aaaggttcaa gatagcaaag atcatatcat gcacagtgag tatttcgaag
     4141 ttagtgaaga agctagcgga gctcttaaca aggccaagag cgaaggccgc aggatcatcg
     4201 ccgtcggcac gacatcgacg cggacgttag aagccgtcta tcaaaagtat ggccactttg
     4261 tcgcgtgcca ggaggcgacc gacatcttta ttagccccgg ctacaaattt gtcgccatcg
     4321 atgccctgat cactaatttt catcttccca gatcaacttt aatcatgctc gtctccgcct
     4381 ttatcggacg cgaatttact atgcaaattt atgctcatgc gataaaagaa agatatcgct
     4441 tctttagctt tggcgacgcc atgttcatct atggaaaaca aaaagaatta ttatcttgag
     4501 tcactgaagg aaattgagaa actcgataaa agcgggccaa aacccactct tttactgcac
     4561 gcctgctgcg cgccatgctc gacttttacg cttcttttct tgtgcccgca ttttgatata
     4621 actatctttt ataacaactc aaatatttat ccgcttagcg agtttgaaaa gcggcgggac
     4681 gaactgaaaa atttcttgaa ggattttaag aaaaaggaag ggcataaagt cgcgctgatc
     4741 ataccggaat ataacaatga tcaatataat attgaattgg aaccatatgc cgatctcccc
     4801 gaaggctcaa agcgctgttt tatctgctac gaaaaaagga tggatgaagc ttatgcttac
     4861 gccgaacaaa acggcttcgc ctattttgcg accgcgatga cgatttcccg ccagaaaaat
     4921 tcgcagatac tgaacgcgat cggcgagaag ctctccacac gatatccaaa gaccaaatac
     4981 ttccacagcg atttcaagaa gaataaaggt atcgacatcg ccaaagccat gcgcgatgaa
     5041 tacggccttt atcagcagac ttactgcggc tgcaaatata catttttaaa gaaaaaataa
     5101 tcgttttttg tctctttcta tgtttttcgg aattgcggca ttcgcatttt ttgatcagaa
     5161 atcagcaatc gttgaagtca tttccaattc gctttttaga gtataaataa tatttatgct
     5221 tgactcaaat acacacacag gggtagtata taaaacgttg gcgtctgcgt tgacatgcga
     5281 ggttgccgat acacccacag gcgttgtcat gaatgggtat cgggtaattc gcacagagca
     5341 agattccaca gcctggaaac acaaggagga aagttcatgg ctaaaaccaa agtaattagg
     5401 gttcgcctca aagcttacga tcacaagata cttgatctga gcgtcgagaa aattcttgaa
     5461 gcggcgaata agacgggtgc tggtgtcgtc ggccccattc cgctccctac ggaaaaacaa
     5521 gtcttcacga tccttcgcgc cgtccacaag tacaaggacg cccgcgaaca attcgaaatc
     5581 aggactcaca aacgactcat cgatattacc aacccaacca aggaaacagt tgacaccctg
     5641 cgtcggctcg acttgccaag cggtgtagat atcgacatca aattataaag gaggtaagac
     5701 tgatgaaatc aatcattgga cgtaaaatgg gcatgactca agtcttcgct gaagacggca
     5761 ccatgtaccc cgtcaccgtc attgaagtct taccaaatgt cgtcactcag ctaaaaaccg
     5821 ccgacaaaga aggctatacc gccgtccaag tcggttatga agatgagcgt gaatcacggg
     5881 ccaacaaatg cgaaaaagga cactttgcca aagcgaatgt cacccccaag aagcatttgg
     5941 ccgaacttca cggcgacgaa ctggcaggtt acaaagtcgg agacgcaatt accgcggatt
     6001 tattccatgc gggcgaaatt gtcgatgtca tcggcgtgtc gaagggcaga ggttttctcg
     6061 gttccattca gcgctacgga cataaaattg gaccgaaagg tcacggttcc ggatatcaca
     6121 gaggcgttgg ttccatggct cttaacggcc gtcccaacaa ccgcgtgctc ccgggcaaga
     6181 agatggctgg tcaccacggc aatcaaagcg ccaccatcct caatctcctc gtcatcaaag
     6241 tcgacgccga gaaaaactac atcttagtca aaggatcggt acccggcgcg aaaaagtcgc
     6301 tcgtcacgat tcgcagtgcc gtcaaggaac aacttggcga aaagcagatt gtcaaagatt
     6361 taataaaccg cacccctgcg gtgaatgccg ttaaagaaga agtcgctccg gccgctcctg
     6421 aagttaagga agcgcccgcg gtcagcgaag cgaaaggaga ataaccatgg ctgaaaataa
     6481 aaaagtcagt gtcccggttt acaaccaact aggtgaaaaa gtctccaccg tttccctcaa
     6541 aggcgaagtc ttcaatgtcg aagtcaacaa cagcgcgatg ttcgatgccg tcaatgtcta
     6601 tcgctccaac atgcgccagg cgacttctaa aaccaaaaaa cgcgatgaag tatcaggcgg
     6661 aggcaaaaaa ccctggcgtc aaaaaggcac aggacgcgct cgcgccggca gttcacgctc
     6721 gcccatctgg gttggaggcg gcatcgtctt cggtccgacc ggcgatcaaa actacacgtt
     6781 acagcaaaac aaaaaagaac atcgtcttgc cctcaagtca gcgctgacgc tcaaagttaa
     6841 agacggcctt aaagtcgtcg ataacctcga gttaaaagag attaagacta aatcgtttgt
     6901 cgaaatcgtc aaagccctca aagctgaagg ccgcatcctc gtcgtcctca ccgatgtcga
     6961 agaaaatctc gccctcagcg cccgcaactt gactaatgtg acgattacag tccccaccaa
     7021 tgtgtccgtc tatgacttgc tcaacaatca atcggttatc atgagcaagg cagcggtcaa
     7081 agctctcgag gaggtgctca tctaatggct gcaaagaaaa aagtcgaagc gaagaaagtc
     7141 gaaaaacggt ttgcaaaagc aacccttcaa gactttgata cgattcttcg cccggtcatc
     7201 accgaaaaga cgatggccct catgcaagag aaaaaccagg cgaccttcag agttgccccc
     7261 aatgctaacc gcacacaagt taagttagcc ttcgaacgcg tgttcggagt caaagctacg
     7321 gaagttcgca ttctaaacgt ccttcccaag agtacccgcc ggggcggccg ctatgaaggc
     7381 aagatctccg ggtacaagaa agcgatcgtg accgtcgcca ccggcgaagc cctcgatctc
     7441 ttcaaggaat agcctccatg gctcattata ggagaaatta aatgtcaatc agaagttata
     7501 aaccgacgac gccaagccgt cgcaacatga cgaactcgac gtttgaagaa atcactaaaa
     7561 gcactcctga aaaaagtctt ctcagcaagc tgacaaagac cggcggccgg aataaccaag
     7621 gtgttattac cacgcgccac atcggtggcg gccacaaacg caagtaccgc caaatcgatt
     7681 tcaagagaaa caaagatgat ctcgtcgcca tcgtcaaggc cgtcgaatat gaccccaatc
     7741 gcaatgccaa catctgcctc gtcagttatc tcgacggcga gaaaagatac atcctggcgc
     7801 ccaaagactt aaaagtcggg gatcgcatca tctcgagcaa agtggccgat attcaaatcg
     7861 gcaacgccat ggaactcggc aacattcctg aaggaacagt cgtccataac gttgagttaa
     7921 gtcccggcaa gggcggtcag ctctgtcgcg ccgcgggcac aagcgctcaa gtattaggta
     7981 aagaagacaa atatgtcatc gtccgcctcg ccagcggtga aatgcgcaag attctgcaaa
     8041 cttgccgcgc caccatcgga agcgtcggaa acgaagacta cagcctcatc aacaaaggca
     8101 aagctggcaa aacgcgctgg caaggcactc gcccgaccgt ccgcggttcc gtcatgaacc
     8161 cggttgacca cccccacggc ggtggtgaag gtaaatcacc ggttggacgc gatgcgcccc
     8221 gtaccccttg gggtaaacgc gccctcggcg tcaaaacgag aaaaaccaag aaagcatcga
     8281 cgcggctcat cgtcagacgt cgcaatgcta aatagaagcg aaaggagtaa tcaattatgg
     8341 cacgtagttt gaaaaaagga ccgttctgcg acgctcactt aatgaataag gtgcgcaagc
     8401 tgaacgccgc caaccgcaaa gagattatta agacgtggtc acgtcgcagt accatcttcc
     8461 ccgaattcat tgaacacact ttcgccgtct ataacggccg tgaacacatc accgtatatg
     8521 ttaccgaaga tatggtcggt cacaaattgg gcgaattcgc cccgaccaga acctaccgcg
     8581 gtcacaccgg tcacaccgcc gaagacaaag cagccacggc tgccaaataa ggaggaccta
     8641 tggcagaaaa gaaaccagta actcgcaaaa aagccgtcaa accggccaaa gtcgaggaaa
     8701 aatccgccgc tccggccaaa cccaaagtta ccgaagcccg cgccgttgcc tatgatgtcc
     8761 gcctcactcc gcgcaaagcc cgcatggtca tcgacttagt ccgcggcaaa gatctgaacg
     8821 tcgcccttgg catcctcgcc aacgtcaacc gttccgcttc gcccgccgtc agcaaagtca
     8881 tcaaaagtgc cgcggccaac gccatcaaca acttcgctat ggaagaaagc aaactttaca
     8941 ttcacaccat ctacgctaac gatggcttga aaatgaaaag attcatgccg cgcgctaaag
     9001 gttcatcgtc aggcatcatc aaacggacat gtcacattta tgtgacaatc aagagcagat
     9061 aggagcagca catgggacaa aaagttaatc cagtcggact acgcgtcggc atcaacaagg
     9121 attggaattc tcgttggttc gccgatcgca aagaataccc cgccttctta aaagaagaca
     9181 tggtcatccg tcagtatctt gaaaagaacc tcaaagccgc tctcctctcg cacatcgaga
     9241 tcgaaagagc taaaaccgat aaaggccaca ccgtcaacat cttctgtttc gtcgcccgtc
     9301 cgggcgtcgt catcggacaa gacggagcca atatcaaccg catcaaaaaa gaactcggta
     9361 agaaaatagc gaccgctaat gttcgcatcg acatcgtgga agtcaaaaat cccgatctcg
     9421 acgctcatct cgtcgctcaa tggatcgcct cggagttaga aaaccgcgcc tcgtttagaa
     9481 cggcgcagaa aaaagccatt caaagagtcc gcaaggccgg cggcaaaggt atcaaaaccg
     9541 aagtctcggg ccgcttaggc ggaaacgata tcgccaggag cgaaggttat aaagaaggca
     9601 ttattcctct tcataccttg cgcagtgata ttgattatgc gcatacggaa gcaactacca
     9661 cttacggccg gctcggcgtc aaagtctgga tctgccgcgg tgaaattctt cccgaagaga
     9721 agaaaacgaa aacggaagga gaataacatc gatgctacaa ccgaaaagag tcaaatatcg
     9781 gcgtccgcac ggaattagct acgaaggaaa atcaaaggcc ggcaacgaaa tcgcctttgg
     9841 tgagtacggt ctccaagccg tgaccggcgg gtggattaac gatcgtcaga ttgaagccgc
     9901 tcgtatcgtg ctgagccgtt atacgcagag agccgggaaa gtatggattc gcatcttccc
     9961 gcatctggca aaaacaaaaa aaccagccga agtccgcatg ggttccggca aaggcaatcc
    10021 tgaaagctgg gttgccgtcg taagaacgaa ccgcattatg tttgagattg gcggcgtcga
    10081 cgaaaaggat gcgcgcgaag cgctccgcct cgccgcctac aaactgccga tcaagtgcaa
    10141 ggtcgtcgcg aaggaagcga agggtgagta attatgaaaa ttagcgaact gcgcgaaatg
    10201 agtaacgaag aacttaacga aaaagtttac ttgctcaaag aacaactatt caatcttcgc
    10261 cgcaaaaaag ccgtcggcca attagagcac ggcgaagaaa tccgcagagt ccgcaaggac
    10321 attgccaaag cctacaccat tcagcgcgag cgcgaattag gcattaaata aggaggaacc
    10381 gaaacatgga aaacagagtc gcacgtccga ccttcaccgg cgtcgtcgtc agtgacaaaa
    10441 tggacaaaac aatcgtcgtt ttggttcaga cccacaaaaa acacgccaaa tacatcaagc
    10501 gcgtcaaata cggcaaaaag tactatgccc acgatgagaa taacgaagcc aaagtcggcg
    10561 acaccgtcac gattatgggc tgccggcgcc taagcgccac caagcgttat cgcttagtga
    10621 gcatcgacct caaagccgtc gaatcgatca aggccgctga agccgaactt ctcctcgagg
    10681 aaaaagaggg cgtcaacgcc gtcgaacccg aagcggaagt caaagctgaa gctggcgaag
    10741 agaagaaagc agaataggag cagactatgg ttcaaaatga aacaaatcta gtcgtcgctg
    10801 acaattctgg cgcgaaaagc gtgcgcatct tccgcctttt tggcggtggt aaccgcaaat
    10861 cttcgtccat cggcgacatc gtcatgtgcg cggtcaaaac cgccacgcct aacggcaaag
    10921 tcaaaaaagg tgaaatcgtc cgcggcgtca tcgtccgcac gaaataccct atcaaccgcc
    10981 ccgacggttc gaccatcaac tttgatgata acgcgattgt tctcatcaac gaagacggcg
    11041 ctcctctcgg cacgcgcgtc ttcggtcctg tcgcccgcga attacgcgat aaaggcgaag
    11101 gattcatgaa gatcgtctcc ttggcgaccg aagtattata aggagtgaac ccatgagaat
    11161 taaaaaaggt gacaaagtca tcgtcatcac cggcggcgac aaaggcaaag aaggcatcgt
    11221 tcaagccgtc ttccctaaaa cgcacaaggt ggtcgtcgaa ggcgtcaacg ttcacaaaaa
    11281 acaccgcaag ccgacccaga agacaccgga gggttccatc gttgagatct atgtccccat
    11341 cgatgtcagc aacgtcgccc tcatcgatcc caaaaccaaa aaagccacgc gcgtgactta
    11401 taaagtggaa aaagatcaaa aggtccgcat cgctaaagcc agcggatcca aattagatta
    11461 aggaggtagc taagttatgg aagaagaaaa gaaaaccgta gccccaaaag ctaccgcgac
    11521 aaaaaaagcc gccgctccaa aagaagcagc cgccaaaaaa actgccgccc ccaaggcgaa
    11581 agttaaaaaa gacgaagtaa aagaaaccaa ggttgtcaaa gaagctccaa aagccgctgc
    11641 cccaaaagcc gaagcaaaag ccgaagctcc taaggttgaa gcccccaaag cccccaaggc
    11701 cgaagtgaaa aaagaggctc ctaaagccga accaaaagtt aaggttacca atcgtctaaa
    11761 agtaaaatac gagcaagtcg ttctccccaa attgatggcc aagtacaact tcaaatccgt
    11821 catggagagc cccagacttg ataaaatcgt tatcaatgtc ggtgtcggag acgccatcgt
    11881 caacgccaaa gtgctagatg attcagtccg cgaactgcgc gaaatcgccg gtcagcaacc
    11941 cgtcatcacc aaggccaaga aatcaatcgc caacttcaaa ttaagagaag gccaggccat
    12001 cggttgcaaa gtgaccttgc gcggtacgag aatgtatgat ttcctcgata agctcgtctc
    12061 catcgcccta cctcgcgtcc gcgacttccg cggtgtgagc cgcaacgcct tcgatggcca
    12121 cggcaactac accctcggcg tcaaagagca gttgatcttt cccgaaatcg attatgacaa
    12181 ggtcaacaaa atcagaggga tggatatcgt tatcgtcacg acggcgcgaa acgatgatca
    12241 ggcgttcact ttattaagtg aactcggaat gccgttccgc aaataggagg agagaacatg
    12301 gccaaaacaa gtcaaaaggt ccgctgggcc cgaccgaaga aattcaaagt tcgtgaatac
    12361 acgcgctgcg ttcgctgtgg tcgtcctcac gccgtctatt caaagtttca tttatgccgc
    12421 atctgcctgc gcgaattagc ctataaaggc gaaatcccag gtcttaagaa gtcgagctgg
    12481 taaaggagga aagaaacatg atgacagatc ccatcgccgc catgcttacg accattcgta
    12541 atgctaatgc gctgcgctat gaaagtgtta ccgttccttc gagcaagatg aaggtggaaa
    12601 tcgccaagat tctcctcgaa gaaggtttta tcctcgacta caaagttctc ggcgataaga
    12661 aaaaagattt aagtttaacc ctcaaatacg ccgccaacaa agagcgcgtc atctctggtt
    12721 tgaaacgcat ttccaaaccc ggtctccgcg tcaccgtcgg ctgcgaaaat ctcccccgcg
    12781 tcttaagagg actcggcgtg gcgatcatct cgacgagcaa aggagtcatg agtgacaaaa
    12841 aagcccgaac cttagggatc ggcggcgaag ttgtcgctta tgtgtggtaa ttgatatgtc
    12901 acgtatcggc tataaagtta tcgatctccc gagcaccgtc accgtcaccg tcaatgacgg
    12961 cgtcgctctc gtcaaaggtc ccaaaggaac tctggaagtc aaggttcccg cctctatcga
    13021 cttaaaaatc gaaggcgctt taattcacgt ttcgcgtgaa aacgaagagc gccagaccaa
    13081 agaaaatcac ggcaccttcc gcgctcttct tcacaacgcc atcgtcggcg tttccgaagg
    13141 cttcagcaag acgcttgaaa tcgtcggcat cggctatcgg gccgcgctta aaggtcatga
    13201 tttactcttg aatattggct ttgcccacga tgtaatcgtg actccggaac cgggtgttaa
    13261 aatcacctgc aagacggcca acatcgtcat cgttgaaggc atcgaccgcc aggcggttgg
    13321 ccaaaccgcc gcccgcatcc gcgaagttcg caaacccgaa ccttacggcg gcaaaggcat
    13381 taaatatgcc ggcgaacaca tcattcgcaa ggaaggcaag cgcgccagcg ctaagaaata
    13441 gtgggaggta aggtatgatt aaaaaattat ctaaaaatgt cgtccgtcaa agacgccacg
    13501 cccgtgttcg cgagaagatc tccggcaccg cggaaatgcc ccgcctctgc gtctatcgct
    13561 ccaacgccaa catcgaagct caaatcatcg atgatgtcaa aggagttacc ttagctcacg
    13621 cttcttcctc ggctttaaaa ctcgcgaacg gcggcaacgt cgaagcggcg gctaaagtcg
    13681 gcgaagcaat cgctaaagtc gcattagaaa agaacatcaa aaccatcgtc tttgatcgcg
    13741 gtggttatgc ctatcacggc cgcgtgaagg ccctggctga agccgcccgc aaagccggct
    13801 tgcaatttta aggagaaaga acatggtaga aaaagaactt gaaggcgtcg ctacagtcgc
    13861 cgcaactccc gaagctccag gtgccgctcc tgaaaaacca gcttttaacc gcggtccccg
    13921 cggtccccgc ggtccccgcc cccaaggcga acgccgtccc ggccgccgtc cgatgcgaga
    13981 tgagcccaaa gaatacgaag agcgcgtcgt cagcgtcaac cgcgtcagca agacggtcaa
    14041 aggcggacgc aaaatgcgct tctccgtcct cgtcgtcatc ggtaacggca aaggcaaata
    14101 cggttttgct attggcaaag ccgccgaagt ccccgatgcc attaaaaagg ctcttgaaaa
    14161 agcccgcagc aacaccttta cttttaagac ttccaaaggc gatacgatcg cccatgaaat
    14221 catcggtaag ttcggcgcct gcagcgtctt tctaaagccc gcgaccgaag gtaccggtat
    14281 tatcgccggc ggcccggttc gtgctatcct tgaactagcc ggcatccgca atgtctactc
    14341 gaaagtttac ggcagccgcg ctcccatcaa catcattcgc gccacgcacc atggtttaag
    14401 caatcttaaa tcatatgcga aggtcaaaga attgcgcaaa taacctggag gcatgaacat
    14461 gaagctacat gaattagaac acgccgaagg cgcccgaacc gctcattacc gcaagggtcg
    14521 cggcatcggt tccggaaacg gcaagacatc cggcaaaggt cacaaaggcc aaggcgcccg
    14581 caccggcggc aaaaaagctc ccggtttcga aggcggtcag aacccgattt atcgccgttt
    14641 gccgaaattc ggttttcaga acatcaaccg cgtcgaattc gccagcgtca acatcgagag
    14701 attaaacgcg tttgaagacg gcacggtcgt gacctcggct ctcctcgtcg aaaaaggcct
    14761 cgtccgcaag gaatacgacg gagtaaaaat cctcggaggc ggaaagttga ccaaaaaact
    14821 gacggtgcaa gcccacaagt tttcgaagtc cgcaaaagag gcgattgaaa gcgccggtgg
    14881 caccgtcgag gtaatttaac atgaagaagc tggtctcggt cttcaaaaat aaggaaatcg
    14941 tcagtcgcat tttcttcacg atcatgatct tgttcgtgat tcgcatcgga gcggccatca
    15001 ccgttcccgg tgtgaccgtc agcgaggaat tatccaaggc gcttgaaacc gccaatatga
    15061 tctcgatgat ggatatgctc ggcggcggtg ctctcagcaa tttctcaatc tttgctctgg
    15121 gcgtcagtcc ttacattacg gccggaatta ttattcagct tctgtccaaa gacgttttgc
    15181 cggctctgac cgaacttacc aagcagggtc agtacggccg caaaaagtcg gagatggcca
    15241 cccgttatct cactctcctt ttaggagctg ttcaggccta tggcatcatc aaaacgatgg
    15301 agaacagcca gtatatcacc atcaacatgg aactcaattt ctgggcttat gcctatattg
    15361 tcaccatcat cctcgccgga gcgatgttga cgatgtggct cggtgaccaa atcacctcca
    15421 aaggcctcgg aaacggtatt tccgtcatca tcttcgccgg tatcgtccgc aatctcccga
    15481 ttcagattgc caccgcttat cgcaagtggg tcgaacagaa tatgggacac ggcacgaatg
    15541 aactcgtcaa aggctctttc caattcggaa tttacatcct ggcattcgtc ttgatcatcg
    15601 ccttcgtcgt cttcatcgag ctgtccaagc gcaagattcc cgtccagcac tccggcaaaa
    15661 gcggcggtca ggggcaatcg atgtcgaaag cctcgttcct accgattaaa ctcaactctt
    15721 caggcgttat ccccgtcatc ttcgcgagct cgattttaat ggcgccatcc gtcatcgcaa
    15781 gtttcgtgag cacggatacc aacgccgcct ggctcaagat tttctcctat agcgaaatga
    15841 ccgagatgcc gggtatcaac gacacgacat ggggcatgcc ctggggcctg ctcttatatt
    15901 taatcttaat cgtcgtgttc tgcttctttt acgccaacat gcaaatcgcc ccggaaaccc
    15961 tggctgataa ctttcggaaa aacggcagtt acattcccgg cattcgcccc ggaatcgaaa
    16021 ctgagcgcta tgtcaagaaa gtcgtcaacc gcgtcaccgt catgggtgcg atcgccctgg
    16081 ccttcatcgc ggccttaccc gtcgtcttga cgctgagcgg catctttggt gagagttcaa
    16141 gtctcgccat cggcggcaca ggcttaatca tcgtggccgg tgtggctatc gaaataaaca
    16201 accagattga cggcttatta gccggcaaga gcttcgagga agcctcgcaa ggagcgcgcc
    16261 gccgatgaat attatcctga tgggcccgcc cggcgcgggc aaaggcacgc aagctaaacg
    16321 cttgattgcc aaatacgata taccgcatat ttccaccgga gatatgtttc gcgaggcgat
    16381 gaagcagggc accgcgctcg gcttactcgc caagcggtat atagaccagg gacatcttgt
    16441 tcctgatgat gtgacaattg gtatcgtccg tgagcgactg gcgaaacaag attgtcagaa
    16501 aggctatctt ctcgacggtt ttccgcggac gattccccaa gctgaagctt tggaaatcct
    16561 gaccaaagaa atcggtcgcg aactagaatt cgtcatcaat atcgacgccg acgaaaaaga
    16621 gctcgtccgc cgcatatgtg gaagacgcgt atgcaaagtg tgcggaacgc cctatcacgt
    16681 cgacacgatg aagccgaaag tcgatggtgt ctgcgatgtg tgcggcggtc ctttaatcca
    16741 aagagccgac gacaacgagg aagcgctgaa agtgcgcctc ggccactact atgaacagac
    16801 taagccattg cttgatttct atgcaaaacg cggccttctg acgtcatttg acggcacaaa
    16861 agacctcaac gtcctgcttc aggaaatctc ttcgctcatc gaggctaaga ctagatgatc
    16921 atcgtcaaat cgccgcgaga aattgccctc atgcgcgagg ccggacaagt cgtcgccaaa
    16981 gtctttgaaa aactagcgac catcatccgc cccggcatca gcacgcaaac gatcgccgat
    17041 gaagccgaaa agattattcg aagttatcat gcctcgccca ccttccttgg ctacaacggc
    17101 tttaagggcg ccatctgcgt cagcgtcaat gacacgctcg tccatggcat tccgaacaaa
    17161 catcacttat taaaagaagg cgatatcgtc tcctgtgatg tcggcgccac ccttaaaggt
    17221 tatgtcggtg atgcctgccg gacgtatccg gtcggcaaga tcagcaagga agcggctcgt
    17281 ttgcttcgcg tcacggaaga gagtttcttc aaggcggtgg ccctcattaa accgggcatc
    17341 cacttaggcg acatctccca cgcgattcaa acccacaacg aggaaaacgg ttattccctc
    17401 cctcgggaat atacaggtca cggcatcggt caagccctgc acgaagaccc ctatattcca
    17461 aatattggcg aaccccatac ggggccagta ttaaaagaag gaatgtgcct cgccatcgaa
    17521 ccgatggtcg cccagggtca gcccgaggtg atcgtcagca acgatggttg gaccgttaag
    17581 atgaaagacg gtttgctctc gagtcattac gagaacacgg tcgccgtcac cagcaccggt
    17641 tatgaaatac tgaccatgct caaacaagga ggaaacgaaa atggctaaaa cagatgtcat
    17701 tgaagtcgaa gcaacagtcg tcgaggcctt gcccaacgcc caatttaaag tcaaacttga
    17761 aaacggcgtc actattcacg cccatgtctc ggggaaaatc cgcatgcatt acatccgcat
    17821 tctcccgggc gatgtcgtca cggtcgaaat ctcaccctac gacttaacga gaggacgtat
    17881 cacctttaga tacaaaaacc caagctcttc atttacgaag aaataacacg gaggaacaat
    17941 ttatgaaagt agcaccttca gtcaaaccga tctgcgataa atgcaaggtc atcaaacgca
    18001 aaggcaaagt catggtcatt tgcgagaatc ccaagcacaa gcagagacaa ggttaaaagg
    18061 agtaaaacaa atttatggca cgtattgtcg gagtagacat acccaacgaa aaaagagtcg
    18121 tcatcgccct cacctacatt tttggtatcg ggacgacgaa aagcaaaaag attttagccg
    18181 cctgcaatgt cagcgaggat attcgcgtta aaaacctgag cgaagaacag ctgactttga
    18241 ttcgcgctga agttgccaag caccgcgtcg aaggcgatct tcgccgcgaa gtcgctttaa
    18301 acatcaaaag acttacggaa atcggctgct atcgcggcat ccgtcaccgc aaaggcttgc
    18361 cggttcgcgg ccagagaaca agaaccaatg cccgcacccg caaaggcaag agaaaaacca
    18421 tcgccaacaa gaaagtggcc gccaaatagt aggagggcat aaaaatggca aaagctaaga
    18481 aaacaaccac tcgtaaaaag aagattaaac ggtcgtttac caagggcgtc gcgcacgtcc
    18541 actcgacctt taataacacc atcgtgacga ttaccgatga actcggtaac gccatcgcct
    18601 ggagcagcgc cggagccctc ggcttcaaag gcgccaagaa atcgactcct ttcgccgccg
    18661 gtctcgccgg cgaagccgcc gccaagattg cctttgaaca aggcctcaga agcgtcgatg
    18721 tcaacgtcaa aggtccgggc ccgggtcgcg aatccgccat ccgctcgtta caagccgccg
    18781 gcctcgcgat tcagacaatt accgatacga ccccgattcc gcataacgga tgccgtccgc
    18841 caaagcgtcc tcgcggttaa tgaaaggaga tagtcacaat ggcaaaatta ccccgtccgt
    18901 ttgaaaaaat gacattcaaa accgctatcg tcgatagcac ttcgaactat ggcaagttcg
    18961 ttatcgaacc gctcgaaaga ggtttcggcc taaccgtcgg aaacgctctt cgccgcgtgt
    19021 tattaagcgc cctgcccggc gccagcgtct acgccgtcga aatcgaaggc gcccgccatg
    19081 aattctcagc ccttcccggc attgaagaag acgtgacagc cattatctta aatcttaaag
    19141 atttagtatt aaagattgac gatgatgacg aaaaatccaa gaagattgaa gtcgacatca
    19201 aaggccccgg cgcccttaaa gccggcgacc tcgtcttgcc cgctttcgtc tcggtcgtca
    19261 accccgactt agagctcgct catctcaccg acgaaggtca cctgaagatg acgatgtttg
    19321 cccgcaacgg ccgcggttac gtgaccagcg aaggcaacaa actcgcccgc acccaccagc
    19381 aagtgggcgt catcgccacc gactccaact attcgccgat caccaaggcc agttatgtcg
    19441 ttgaaccgac tcgcgtcgga cacgactcga acttcgaccg cctgaccctt gaagtgtgga
    19501 cgaacggttc catgactccg caggatgccg tcgctttatc aagccgcatc ttgatcgggc
    19561 acttcgaaga attcctgaag cttcaagagt taactcgtga tatcaacatt atcaaagagg
    19621 ttgtcgaaac cccggtcgat gatgtcggcg acaaagcgat cgaagaactc gatctctccg
    19681 tccgcagcta caactgcctg aagagagccg gcattcagac gattcgtgaa ctcattcaaa
    19741 agagcgaaga agaaatgatg aaagtccgta acctcggcaa gaaatcatta aaagaaatta
    19801 aagataagtt agtcgagatg ggactcagtt tccgcgacta catcgcagag taggaggcac
    19861 catggcagca caaggacgta aaaacgtaca cggcaaaacc ggtgtccgct tcaaagccgc
    19921 ttataccgcg agcaaacgca aaaacatgct ccgcaacgtc gtcaccgaac tgattgttca
    19981 cgaaaaagtc gtcgttacct caggagttgt caaagagctc aaaaaactcg ctgatcacct
    20041 catttccctt gcaaaacgcg gggatttgca cgccagacgg caggccgcca gcgttctccg
    20101 caaggtcgtc atcgatgaga aagaaagcct taccgcttta gataaattgt ttgatgatct
    20161 cggcccggcc ttcaaggccc gcaacggcgg ttataccaga gcgctcaaag ccgataaccg
    20221 gcgcggcgat aacgccaaac gcgtattggt gacctgggtt cgctaggata caaaaccgat
    20281 caaaacggct cgcaagggcc gttttttcat gtcaagacgc ctgtgcaggc atatcttttt
    20341 tatcgtaaat cgaggtgcta caatgaattt aaatataagg agtttcccat gagcaagaaa
    20401 tatttgcagg aactagaacc gggctatacg cctctaacgg tttgtgagga ggcgagccgg
    20461 tgccttcttt gccttgacgc tccgtgttca aaagattgcc cggccggcac cgatcccggc
    20521 cgctttatcc gcgcggtgag attccgcaat tttaagggtg ccgccgaagt cgtccgtgaa
    20581 aataatgttc tcggttcgat ttgtgctcgc gtctgtccga ccgaaagata ttgccaaaaa
    20641 gggtgcgctc gctcgggaat tgatgttccg atcgatatcg gccgcattca gcgctttatc
    20701 accgactttg aagaaactaa tggcatgaaa atcctcaaaa aaggaaaacc gaacggcaag
    20761 cgcgttgcga tcatcggaag tggcccggcc gccctgcagg cagcggcctc tctcgcccgc
    20821 ctcggctacg aaattaatct atatgaaaaa gaagcaaagg ccggcggata tcttcgctat
    20881 ggcatcccag aataccgcct tccgacgcat atcgtcgata aagaaattga gtacgtgacc
    20941 gaattagggg ttaaaattca cttaaatacg acagtcggaa aggatatcag cctcgataaa
    21001 ctgaagagcg ataatgacgc cgtcatcgtc gccgtcgggg cgagctacgg caagatgctc
    21061 ccaaaatttg aaaataatcc gcatgttgaa attgccgtcg attttttaaa gagggttaaa
    21121 gacagcaaag ggcaagttga tttaccgaat aatatcctcg tcatcggcgg aggagatgtc
    21181 gcgatggatg ttaatgtgac gctcaaatta ttaggcgtta aaaacatcag cgatgtggtc
    21241 tatgaggagt tttgcgaatt ccgggcatcg aaaaaagaac tacgcgatgc ccaggccctc
    21301 ggcgtatcga ttatcgacgg ctatgtgcca gtcgatgtcg atggtaaaac cgtcaccttt
    21361 aaacatcgca cgattcctgg cgaagtaaaa ataaccgccg ataaaattat tctggcggtc
    21421 ggccagtatc ccaacgtctg cggcctcgaa ggcattcgca ttgtaaatgg cgagtcggac
    21481 atcaaaggct atgtcaccac cgatccaaaa gtatttgtcg ccggcgatat cgctcacctc
    21541 gataaaacgg ttgtggcggc ggtgcggacc ggcaaggatg tcgcctcgct gattcacagc
    21601 cgcatcggag gaagataaga tgattaagaa agatttgtcg attaactttt taggcgttcg
    21661 ttttcccaat cctttttgcc tttcctcttc gcctgtcgga aattgctacg atatgtgtaa
    21721 aaaagcctat ggcgcgggat ggggtggcgt cgtctttaaa acgatgggct actacaattt
    21781 cgtcgttgac gaagtatcgc cgcgctttga caagttgaac aaagaaaata cgccgtttgt
    21841 cggcttcaag aatatggagc agattgccga gcatcccctc gatgagaatc tggccgatat
    21901 ccgccgcctc aaaaaagaat ttccctatca ggttttaatc gcctcaatca tgggctcgaa
    21961 cgacaaagaa tgggaggagc tcgcacgcct tgctgaagag gcgggagcgg atatgttgga
    22021 gatgaatctt tcctgcccgc agatgacctc acacgcgatg ggaagcgatg tcggaacgaa
    22081 tcccgattta tgccgtcgct actgccaggc cgtcaagcgc ggaagcaaac tccccgtcct
    22141 cgccaaaatg acgccgaaca tcaccgatat gattccggtc gccatcgcct gtctcgaggg
    22201 cggcgccgat ggcatcgccg caatcaatac cgtaaaatca atatgtaata ttgacctcga
    22261 taggaaagtc gggatgccga ttattaacgg caaatcgtca atctccggct attcaggtaa
    22321 agccgtcaag ccgatcgccc tgcgttttat tcagcagatg cgagctgatg agcggctgaa
    22381 aaatgtcgcc atttccggca tcggcggcat cgagacgtgg gaagatgcgg tggagtttat
    22441 cctcgtcggt tcctcaaccc tgcaaatcac gacggcaatc atgcaatatg gctaccgcat
    22501 catcgatgat ttgcgaaacg gactcatgca ttatatggat gagcagggcg tcgatcatct
    22561 cagcgattta gtcggattgg ccaacgataa tctcattccc gctgaagaac tcgaccgcga
    22621 ttacaagatc tatccggaga taaatgtcga tgaatgcatt aaatgtgggc gctgctacat
    22681 atcctgctat gatggcgctc atcaggcgat cgaatgggat gccgataagc gcctgccatc
    22741 cgtcaacaag gagaaatgcg tcggctgttt gctgtgctac cacgtctgtc cttccggggc
    22801 catcaaaatg ggcgaaatcg ccttcaaacc cggccgccaa ggtcagcgca aggccgaaga
    22861 catcattcac tagagacgac caggggtgga aacactcctt tttctttata agaaatgtaa
    22921 gcccatacaa tttttagcgt ccaagtttat attgaaaaat ataagatact tacatattat
    22981 attaaaggtg catttatgcg tcgttttatg acctacggag gataataatg gcagtcattc
    23041 aagaacacgc caagaaaagg attaaagaga tcgtcgcctc tcacaaagac gaaccgaccc
    23101 ctttgctcgt tgtcttgtcg caaatccagc aagaatttgg ttacatccct ctcgaagttc
    23161 aggaagtcgt ttcggaagaa atgaacattc cggtcgccga gatttacggt gtagtcactt
    23221 tttattcgtt tttctcgctc aaacctaaag gcaaatacat tatcggcgtc tgcctcggaa
    23281 ccgcctgcta tgtcaaaggc agtcaaaaca ttatcgataa gttttgcgaa ttgttgaaga
    23341 tcagacctgg cgaaacgact ccggatggtt tatttacggt tgatgctctg cgttgcatcg
    23401 gcgcctgtgc gattgctccg gccgtcacga tcaacggtaa agtatatgcg aatttgacgg
    23461 tcgatcgcgt cgaaaaaatc atcaaagaat atgaaggagg ggccaactga tgagaattaa
    23521 tactcctcaa gaattagcca gcaaaatccg tgcctgcgaa aagtgtttag ctcagaaatt
    23581 tagcggcgaa acaggcacga aagttttact tgtctgcggc ggtctaggtt gcatttcgac
    23641 gggcgccggc gacattatca gcttacttaa aacgaaaatc aaagatcgcg gtctcgaaga
    23701 caaagtcagc gtcgattccg tcggttgttt cggcttctgt tcacaaggac cgttcattcg
    23761 cgtctatccc ggtgagaccc tttatcgcat ggtgaaaccc gaagatgtcg atcgcatcat
    23821 cgaagaggat gtcatcggcg gaaaaatcat cgaagaccta atttacatcg atcccaaaac
    23881 taatcaaccc gctcggactg agaaggatat cgatttcttt aagaagcaaa tcaaaatagc
    23941 tttgtacgga agcggcatca tcaatccaga aagccttgat gaagccttgg gttatggctc
    24001 gttcagagct tttcatcaag tcatcacgac gatgaagagc caggaagtta ttgacgaaat
    24061 tcttaaatcc ggcctgcgcg gccgtggcgg cggtggcttc ccgaccggtc ggaagtggca
    24121 gtttgctctc gatcaaaaat cggatgtcaa atatgtcgtc tgcaacggcg acgaaggcga
    24181 tccgggcgca tttatggatc gttcgattct tgagggtaat cccttcgccg tgattgaagg
    24241 catgatgatc gccggttatg cgattcaggc gacggccggc tatttctatg tccgcgccga
    24301 atatccttta gcggtgtcaa gattaaaaac agccatcgcc ctcttagagg aagctggctt
    24361 gattggcgat aatattttaa ataccggatt caatttccgt gtttatataa gattaggcgc
    24421 cggcgccttt gtctgcggtg aagaaacggc tcttctgaat tcgatcgaag gtcagcgcgg
    24481 ccagcctcgc ccccgcccgc ctttcccggc cgttaagggc ttgtggcaaa aaccgacgat
    24541 tgtcaacaat gttgaaactt tggccgtcgt cccgtttgtc attcgtaacg gcgcggatga
    24601 atttgccaaa ctcggcactg aaaaatccaa aggcacaaaa gtctttgccc tcggcggcaa
    24661 ggtcaataat gtcggcttag tcgaagtccc gatgggcacg acgctgcgcg aactcattta
    24721 tgagatcggc ggcggcattc ctcacggcaa gaaatttaag gcgattcaga ccggcggtcc
    24781 ttccggcggc tgtttaaccg aaaaagattt agatacaccg atcgactatg ataccttgat
    24841 cgcccgcggc tcgatgatgg gttccggcgg cgcgattgtc atggacgaag ataactgtat
    24901 ggttgatgtc gccaagttct acatggaatt tatctgtgat gaatcgtgcg gaaagtgcac
    24961 gccgtgccgt atcggcacca agcgtatgct tgaatacctg gaaaaaatca ccgaaggccg
    25021 cgcctcgctt gaagatctcg atgaactgac cgaattggct catgccgtca aggaaaattc
    25081 tttgtgcggt ctaggtcaga cggcggccaa tcccgtcctt tcgacgatgg ccaacttccg
    25141 cgatgagtat ctcgctcatg tcgtcgacaa aaaatgtccg gcccatgtct gcaagaaatt
    25201 aatggaatat tatatcatcc ctgaaaaatg taaaaagtgc ggtaagtgcg cacgggtttg
    25261 tccggtgcaa tgcatcagtg gtaccttggg caagactcca ttccttatcg accccttaaa
    25321 gtgtattaaa tgcggcatgt gctattcggt ctgcccgttt gattccatca tcaagcgcta
    25381 aaggagaaat agtcatggca aaattagtaa atatcaaaat tgagggcaaa tcctttcaag
    25441 tcgaagaagg cttgacgatt ttaaaagccg ccaaaaagtg cggtttcgat atcccctcac
    25501 tttgtgcctt caatgatggc aagtgttctc tggcgagctg ccgcgtctgc ctcgtcgaag
    25561 ttaaaggcgc ccgcggttta gtcgcttcgt gcgcttatcc ggtcaacgaa gggatggaaa
    25621 tcagcatctc ctctccccgc gccgccaaag cgcgccgttc atcggtcgaa ctgcttttga
    25681 gcaaccacag caagaattgc cagcagtgct ccaaaaacgg tcaatgtgaa ctactccatg
    25741 tcgccaccgt taccggtgct cgcgataatg cctataccgg cgaactaacg agaacaacct
    25801 tagatcgcct cgctccgggc atcgtccgcg acacatccaa atgcgtcatt tgcggccgct
    25861 gcatcgtccg ttgtaaagaa gcgcagggca tcggtattct cggccaggaa ttccgcggtt
    25921 tcaaaaccat cgtttctccg gctgataacc gcagtttcat gcactcacca tgcattcaat
    25981 gcggtcagtg tatcaatgtt tgcccgaccg gagccctgtc agaaaacagc gaaattaatg
    26041 ccgtcgacaa agcttttacg acacacaaat acgtcatcgt ccaaaccgct ccggctgtca
    26101 gagccgctat cgccgaagaa ttcggcgaaa aaatcggcac gcccggaacc ggcaagatgg
    26161 tcgccgcctt gcatcgcctc ggcttttacc gcgtctttga tgtaaacttc tcggctgacc
    26221 taacaattat cgaagaagcc actgaattac tcattaggat gaaaacgggc ggcaaacttc
    26281 cccaaatcac ttcctgttca ccgggctgga ttaactacat gaaatacttc tatccggata
    26341 tcatccctca catctcgaca tgcaaatcgc cgcacgagat gggcggggct atcatcaaat
    26401 cgtattttgc cgagactcac ggacttgatc ctaaggatat cttcgtcgtt tcaatcatgc
    26461 cttgcacagc aaagaaatat gagaagacga gaccgcaaaa cgccgccgtg caaaacctgc
    26521 cggatgtcga tgctgtcttg accactcgtg aattggcaaa gattatcaaa cgagccggca
    26581 ttaactggca gaaattacca gaagagcaat tcgacgatga tttaatcggc gaatacagcg
    26641 gcgccggcgt catctttggc gtcacgggcg gcgtcatgga agctgccctg agaaccgcct
    26701 atcacgaatt gaccggtaaa gaagccgata tcgtggcttt tagtgaagtc cgcggactgg
    26761 aaaatattaa agaagccgag atcgatatca acggcacaaa agtgcgcgtg gcggtcagtc
    26821 atggcatgaa gaacgccaaa ccgcttctcg accagattcg caacggaact tcgccatacc
    26881 attttattga aattatgggt tgcccgggcg gatgtatcaa tggtggcgga cagccattcg
    26941 tcaagccagt atttctgccc aatgaagatg ataacattct tgagacatac atgaagaagc
    27001 gagccagcgt cctctatagc gaagacgaaa aacaagtcgt tcgtcaatcg cacaataaca
    27061 aggatattct ccgtctctac aagaactatc tcggcgaacc ctgcggacac aaagctcacc
    27121 atctcctgca cacctcatac gtggctgaca gagtgcgctt cccggggaaa taaaagattg
    27181 caaatcgagg ccgaaaatca catattcggc ctctttttat aaaaatagta tgtataaagc
    27241 tctccttttt gatctcgacg gaacgattct tcattccatc agcgacatta ccacagccct
    27301 aaatgtttcc ctaaaaactt gcggctacaa tttttcttat gacgatgacg gaaccaagaa
    27361 gctgattgga atgggggccc ggaacatcat cgcccgcgtc tttcaaaatc ggcctcatac
    27421 cgaggaagat tttaatcaac ttctcaaagt tttttcgcat aattacaaga ttcaccaggg
    27481 ccggcaagcg acaccttatg agggtgtgat ttttcttctt cgggagctaa aaaatgccgg
    27541 tgttaaatta ttcgtcgcca gcaacaaacc cgaccacctt gccaaagcga ttgtcgctcg
    27601 gtttttcggc gagaatatct ttgacgctgt ctatggtcac gaagagggca aaccagaaaa
    27661 acccgatcct tatcttgtca atcatcttct gacaacttat catatcgcac ccaatgaagc
    27721 cctcttcatc ggcgacagcg atgtcgatgt tgcgaccgga cataactcga atcttgatgt
    27781 ctgcctcgtc acctggggct atggcgatta ctcgaataaa ctcttcaaag aagccacttt
    27841 tgtcgctcaa agtatttccg atttaaaaaa gattgtcttt gcataaggca agtgttatta
    27901 tttcgccaac cataaacaaa agtatttttt gtttgtgcta taataatttt ttagagtgtg
    27961 aatatggaag tcagaatttt taattcatta actgatcgca ttgaaacttt cgtgccctta
    28021 aaaccacgcg aggtttccat ttatgtttgc ggtccgaccg tctacagcga cccccatatc
    28081 ggaaatctca gaccggtcgt cgtctttgat gttcttcggc gcatgtttat ttcccttggc
    28141 tacaaagtca cttttgtcag caatttcacg gacgtggacg ataaaattat taaccgcgcc
    28201 atcagcgaaa aaaagagcga agaggaaatc tcaaaccatt atatacaagc ttttcgaaac
    28261 gtcgcggaag ccgttcactc cctagctccc gatatcgctc cgcgcgtgac cgaatacatt
    28321 ccggatatca tccgttacat cgaagtactg attgacaaaa aagctgcata cgccgtcgat
    28381 ggcgatgtct atttccgcgt ctcatcgatt gccaattatg gagaactgag caacattaat
    28441 atcgacgatt tgataagcgg cgccagaatc gaagagaaca gcaagaagga atcgcctctt
    28501 gattttgtct tatggaaaaa aacgaccatc ggcatcaatt gggaatcgcg ctggtccagg
    28561 ggccgacccg gctggcacac cgaatgctgc gtgatgatca attccctctt tcctgatggc
    28621 aaaatcgata ttcatggcgg tggttttgat ctcaaatttc cgcatcacga aaacgaaatc
    28681 gctcaaagca aagcacataa taataactcg attgccaact actggatgca caacggattt
    28741 atcaattttg gcgaagataa gatgtcaaaa tctctcggca atgtcatcct tgcaaaagac
    28801 attatcgaaa agtataacgg caacctgatt cgcctcgttc ttttgggcac atattatcgt
    28861 tcccctctca catttacctt tgaagttatg gaaacggcaa aaattgagtt ggaaaaaatt
    28921 gaaaattcat atcgtcaatt agctgtctct ttgcaactgg ccaatttcga tttgagtgag
    28981 gaacaagaga ctgatattcc gcaattcatg gagcatctcg ccaacgactt aaatacgcca
    29041 aacgcactaa ctgagttatt tgctataata aagaaagcca atggcgcttt aaggcaaagt
    29101 tcgcttgatt atttggcgct tcggaaactg ttttcgagta tcggcgccat gctgaatatt
    29161 ctcgggattt ttgttgatta tccacgatta tcaaaagaag ataaacatat gtacactgaa
    29221 tatttaaaat acaaaaacga caaaaactat gcagaaagcg atcgtttacg ccaaattttg
    29281 atggctagac gcattatcta ggaggaaatc ttatgaacgg cattctctca attgatcaac
    29341 agattgtcag ctggtttcac gccttattcg gcgggaatgg atggggcacc atcatcttgg
    29401 ttgtcgcctc cctgctcgtg acgatacttt gcgccggaat catcggtttt gagcgcgagt
    29461 atcacggtca tgccgccggg ctcagaaccc acttgctcgt ggcgatcggc tccgccctca
    29521 tcatgacgat ttccctctat ggctttccgg ttatctttgc ttccgacaca cgcgatcctg
    29581 cccgcctggc ggctcaggtt gtcagcggca tcggcttctt gggagccgga acgataatac
    29641 agactggcac ggatgtcaaa ggtctaacaa cggcgacgac actgtggtta gccatggcga
    29701 tcggcatcgc tgccggaagc ggtaatttta ttattgccat cattgccacc gctctcgctt
    29761 ttgtttctct tgtttctttg cgcaggattg aaaagctggc gagccgccgc aatccgatca
    29821 tccgcatcgt cgtgccaagc gaacggccga ttttaaaaga aatacatctc gtcgccaatc
    29881 attatggcat tgccattcgc gatctcgaat cgcaaattgt cgaataccag aaccagtccg
    29941 tcttgcgttt gaccatgcgc tgctcgtttg tcgccaaagc tcaagtgacg gctttcatcg
    30001 atgatctccg cgaaaaaatt aatcctctcg atatcaaact ctcaaccgaa agttagcaga
    30061 ccgataaggt tgagaaaata attttaaatg acatgtcaaa attaatatac gggagaaatc
    30121 cagtattgag ttccctcggc gaaaatcgcg ttgagaaggt tttcctcgac ttgcatttca
    30181 acgatcagga aatcattcga aagctgagcg cgaaaaagat tgtgattgaa agagttccat
    30241 cttcacaact cgacgacttc gccaatcacg gcgtccatca aggcatcatc gcctcggtcc
    30301 ttgatttccg ctatcactcg ctcgatgaga ttttgaccgc cgccaagagc aagagattcc
    30361 cattgattgt catcttggat tcgctaaaag atccccacaa tctcggagcc atcttgcgga
    30421 catgcgatgc ttttggagtc gacggtgtca tcatcaagaa acgcggacaa gtgccgctaa
    30481 acgcgacggt cgccaaagtt tcaacagggg cgattgacca tgttaaggtt catgaagcca
    30541 gcaatctcag caacgtgctt tcaaaactca aagatgaagg tttctggatt gtctcaagcg
    30601 atggtagcgc ccagatggag tatcatcaaa tcgatgccca gagaccgctc gccctcattc
    30661 tgggaagcga aggtgaaggg gtcagcgaac tcttgcttaa gcgcagcgat tacatcgtca
    30721 aaattcccat gtttggtcac gtcaattcgc tcaatgtttc ggtcgccgcc ggcattctaa
    30781 tcgctagtat tatcgcaaag cgataaatat ctcccgagga ggattaatga gtcattcaca
    30841 agtcagcgat gagcaactga ttttatgcta tcgccaaaat tcaaaagaag cttacgatat
    30901 cttgcttaag cgcaagcatc atgatgtttt gccactctta aaaaaatacg ctaatcagtg
    30961 caaaccgttt ggagttgaaa tgaatgatct ttacgccgtt tatctcgagt cttttcataa
    31021 agctattttg cgatttgtct tcgaaaaaat cacttttcaa acttactttc taaaagttct
    31081 taatcgcgat ttggccggat tctttcgctt ggtatcaaac cccaatattc cgcgtaataa
    31141 ctgctttagt ttagactctg aagtcggtcc tgataccact ctcacctttc atgatgtcct
    31201 cgctgattcc tcacagaaaa tcgatgccag aagttatgtc aaagtcacct cagcctacga
    31261 tttaattaat ggcgaaccaa agaatagccg tgaggaaacc atcaagcgca tcatcatttt
    31321 aaaagttgcc ggttattcga tctcggaaat cgcctcactg acgaatttga agcctgcttc
    31381 gatacgtcgg cgactcagcg gctttaacga cggtgaactc gctgatcagc taaaaaaatg
    31441 cctcatgtaa gcgcgtccgc attcaatcat tgacagacct tttgctgaat gctatatttt
    31501 gtaggcaaga tgtaaggtag gataatcaaa atgatgcgtc aaaaaattat tttaatctgt
    31561 ccgatttgct taaaccgcaa ctacacgacg acgaagcgga aagatgccgc gacgggtcgt
    31621 gtcgagttaa aaaaatactg tccgcactgc aatgcgtaca ccttgcacaa agaaagtaaa
    31681 taggaggctt gttcatggga gttaaaaagt atactgccga agttataaaa gaaggaaagc
    31741 gcgtacgctg gccaaagcga gaggagctgc ttccgacaat tattgtcgtc gtggcaatcg
    31801 ctgcctttgc cgctattttt ctgagccttg aagactgggc ggccggttcg cttctccaaa
    31861 ccttaaaaga agctttcgca gaatggagca aataattatg ggaccagaat taggcaagaa
    31921 gcagtggtac atcgtgacgg catattccgg tcacgaaaac aaagttgccg agaacatcaa
    31981 gacgagaata acccgcatga atctcagcga ctatatcttt aaagtgctcg tcgccgagca
    32041 ggaagtcaac gtcttaaaag acggactgcc aaccggcaaa acaaaaatga aaaatttata
    32101 tcccggctac attttcgtcg agatggtaat gactgacgaa tcatggttta tggttcgtaa
    32161 tacccccgga gtcaccggta tcgccggctc ttccggaggc ggacaaaaac caacgcccgt
    32221 cacccgcgaa gaaattgaac ccgtcttaaa gaggatgggc atcatcgatg aatcgatgta
    32281 tgaccgctac aatgtcggcg atctcgtcaa agttattcac ggtcctcttg agggcaccga
    32341 aggtcgtatc ctgtcgattg ataaagagac cggagcctgc cgcgtcgaaa ccgtcttctt
    32401 cggacggccg acacccgtcg atgtcgaatt cagcgaaatc gaaagaatct aagaaagtcc
    32461 cttcgggggc tttttgttat catatcgctt gtctgtaaca accatttgta ctaactttat
    32521 gttgcgtgcc ttaagcacgg ctacgaggta tcgttatgaa aaaaattata tataccgtcg
    32581 agggtctcga ctgcggaaac tgcgccgcca aaatcgaacg ccatttaaac aaggatgaaa
    32641 caatcgctca tgcgagcctt gattttgcta gcgcccgcct ttatgtgact tataaagaag
    32701 aagcatacaa tctccgcgag ttgacggata aaatcaaaga agtcaatgat gataatctca
    32761 cgatttccta tccggaaaaa gccggtggtg aaaagaagaa agtgatgagc aagaagatca
    32821 tatcgattat cattcgcctt gcgatatcaa cactattatt gctcgtcgcc acatttctcg
    32881 tccctcacga actgcattgg cttcaagttg tcatctattc gctctcctta ggcgtcatca
    32941 tctatgacat cacctggaaa tcaattgcca agatcatccg gcttcgcaat ccgcttgatg
    33001 agaatctcct gctgacgatt tcggcagtcg gtgctttttt aatgggtctt cccatcttag
    33061 ggtctcacgg cgaatacatc gaaggcatta tgattatcgg tttttatcaa atcggacaga
    33121 ttttcgaaga aatcgccatc ttgaaatcgc ggcaggcgat tacaaacgcc gtcgatcttc
    33181 gcggcgatac cgctaatctc cttttagaag atgatataaa ggttgtccat cccaatgagc
    33241 taaaagttaa cgataaaatt gtcattaaag tcggagaaat tattcccgtc gacggactgg
    33301 ttatcgatgg tgaaggaacg cttgacactt caagcctcac cggcgagttt atcccggtca
    33361 atatcggcga aggcgagaaa gtgttgagcg gaagcattct caaaactgga tcgctgaccc
    33421 ttcgagtcga acggcaatat gaagacagcg ccatcgccaa gatactcgat ctcgtcacca
    33481 gcagcggtga acgaaaatcc agcgctgaga aatttattac gaaattcgcc cgcttttata
    33541 ccccggtcgt catgattttg gcaatcctgg tcgctcttgt gcctctcgct tttccaagtc
    33601 aaagttatac ggattggatt tataatgccc tttctgtttt ggttgtttcg tgcccatgtg
    33661 ccatcgtcat ctctgtcccc ctcgccttct tttccggcat cggtctggcc agtaaaaaag
    33721 gcattataat caaaggttca aactaccttg acgaactgag taaattagga ttcatattga
    33781 ctgataaaac cggaacattg acctatggtt attttgaagt taaaaaggcg gttccggtcg
    33841 gtctttccga agccgaattc tgggattatt tagtggcggt ggaaagtcag tcaaatcatc
    33901 cgattgccaa agctatcttg catacccata agcgccaaaa aacccacaag ttgcaacaaa
    33961 attaccgcga attgcctggt tttggcgtag aaaccactta tcaaaaccac cggcttttag
    34021 cgggcaaacc cgcctttcta aaagaagccg gaatcgaagt tccgggcgtc gatgaaatcg
    34081 gcacgatcat ctacctcgcg gtcgatggca actatgccgg ttttgtggtc ctcaacgaca
    34141 cggcgaaagt cgattcaaaa tcgatggtgg ctaaccttaa gaaaatgggt atcaaaacga
    34201 tacttctctc cggtgacaaa aaagacaatg ccgccttgat agcgggagaa ctcaatatcg
    34261 acgagcatta tggttctctg gctcctgaag ataagctcac tattctcgaa tcttatctcg
    34321 ctaaaaataa aaaaggagtc ggctttgtcg gcgacggcgt caacgatgcc ccttcgatta
    34381 tacgaagcga tgtcggcttt gccatgtccg gcatcggtag cgatatcgcc gtccaaaacg
    34441 ccgaagtcat catcgtcaat gacaatccga tgaaggtcgt cgatgccatc cgcatctcaa
    34501 aaatgacgag gtcggtcgct atttttaata tcgtcttttc gctgctggtc aagttttcca
    34561 taatggtttt gatcatcgtc ggagtgctgg gtgaaatgca gatgacaatc gcgatgctcg
    34621 ccgacaccgg attaactgtt ttgatgataa tcaactcgtt gctgcttctt tatcgaaagg
    34681 tataagtttt tataaaaaaa catttttaaa aatgttaaca ttttttaaga agatgaggtt
    34741 tcgcacttcg tcttcttttt attgatattg ccaaaatttt cttgtatgtg ggcatggtat
    34801 ttgttaaaa
//
LOCUS       DAOSSL010000006        29130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1764_length_29130_cov_17.349716, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000006 DAOSSL010000000
VERSION     DAOSSL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 29130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29130
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1764_length_29130_cov_17.349716"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLH17_01390"
     CDS             complement(<1..973)
                     /locus_tag="PLH17_01390"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOR20468.1"
                     /translation="MNKFAIFLKTSLLDPFVRSFQRFTDSIILSILLVIFSIVNLETR
                     GGLAFFGEMLPFLWLTLPFLIFKTLLAERIGLKVLWKYLLTGGALIVTVSFFLLTRFW
                     LTTTDVILSIRLAAAWAIAVLLALIVNYFPKRENFAFFVVFLLTKFFVTIFYAAVLYG
                     GIFAILASIEALFGINLSEFIYIDLFFAVIGFVAVPVFIGFLPSHSEDKQESDYHVIW
                     KTVFAFIVVPLIMIFSAILIVYLITSFVNPNYYGFVYLISALVTAFLTLAMIFLLEKF
                     ETHYPHVHFFNKYWPFVLLAIFAGFVYELVIALLAEGFTLTTSIYLYL"
     gene            complement(1215..2777)
                     /locus_tag="PLH17_01395"
     CDS             complement(1215..2777)
                     /locus_tag="PLH17_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20469.1"
                     /translation="MFFKLLKVFLKENFGARRLFGSKVAKSKGRIIAFALLFVYSFAS
                     IGFSTGMMFYSMGLKRELMLYVASYASGLGFLFALLQANGFLFQFKDYEILGPLPIKP
                     ISLLLAKLKTMMVFIYLVLTALTLPLFIVYYILVPFDLLGFIFLLFGYLLLPLPSIIL
                     GSFLSLLIAKISKRFAKSNLIQTILMFVLFLTIFGFSLISSASSGEGSFIPVWIIDAI
                     SSFYLPNEWYASAVHENNLLNFVYLFLSHAGIFVIFLIAISKLSVKTNQNRTANRQVT
                     IKSNKRQQTPIFIALIKKEWRKFVGTPIYIFNTGFGLIMLAIMAVAAIFFKNELAASL
                     GLFAPFVLLIAYAFCLSTVYTPAISLSLEGNNFGLLKTLPIKGETIMSAKIIFNLLIE
                     APVIIFTFPFAAIGLGLDLWTSLASFLAVISFAVLTSIFFAWLNLFFPRFDFKSEAEI
                     VKQSMSAFIAVFVGFGIIALEGAILFGLIMIPIPLAFPILFLTIINGLLAFLIYWPMH
                     KTASLRLQKIEA"
     gene            complement(2779..3483)
                     /locus_tag="PLH17_01400"
     CDS             complement(2779..3483)
                     /locus_tag="PLH17_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20470.1"
                     /translation="MLNIKNVSKSYDKKKLAINNISMHLEPGDLYGFVGPNGAGKTTL
                     IKSIAGIISFDSGTITFDGHTIAKEPLEFKKQLAYIPDHPDLYETLSGRQFIDFIADA
                     FNVSLERRNQLIGKYAAEFEMLDSLDKPISSYSHGMKQKTALMAALVHEPKLILLDEP
                     FVGLDPKAAFALKKTFKEITDAGTIILFSSHVLEVVEKLCNKIAIIKQGEIIADGKTH
                     EVLKDRSLENFFMEIA"
     gene            complement(3596..4165)
                     /locus_tag="PLH17_01405"
     CDS             complement(3596..4165)
                     /locus_tag="PLH17_01405"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOR20471.1"
                     /translation="MSLFEFRRIADEKLSGKWSEAALVQFLYSLLVGFVTSIGGIGMF
                     LIGGPINIGYAMFIADLYRGERPNIGTLVKGFDERVGDRILLGLISQLYLLLWSMLFL
                     IPGIIKSFSYSMIYFIQLDNPDMHYEDVIMRSRKIMNGNKWRLFCLYLSHIVWYILVL
                     LTFGILTFWVEPKVNLAKYAMYLEIKNNY"
     gene            complement(4262..4588)
                     /locus_tag="PLH17_01410"
     CDS             complement(4262..4588)
                     /locus_tag="PLH17_01410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20472.1"
                     /translation="MEQLIFGKNKDRFYMGESEDKALAEVTFKKHKDNIIILDHTYVN
                     RSLRGRGMGKILVDRVVSYARENNLKIIPTCPFAKDIMEMSKVYADVLMSDATLQEVK
                     DVACPL"
     gene            complement(4749..5126)
                     /locus_tag="PLH17_01415"
     CDS             complement(4749..5126)
                     /locus_tag="PLH17_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20473.1"
                     /translation="MDIFNDERRILSRVNNVRALVMVGRQFGNNIKMHATSFTGVKTI
                     GLFYLRENTSCRIDVDFEVLSGRAKVVLIRKQSIRDIAVSTAREVRIFKLEKGFNRIR
                     LVGEKAEVRLTLVLTKGVTFLDQ"
     gene            complement(5232..6215)
                     /locus_tag="PLH17_01420"
     CDS             complement(5232..6215)
                     /locus_tag="PLH17_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOR20474.1"
                     /translation="MKPLFKGRHFITLEEWTKEEIDKLLEVSYDLKNKFYKNIPTPYL
                     ENKTGFLMFFEQSTRTRNSMESGLAQLGGHATFLDTSMMQISHGESAKDTAVILSSFG
                     HGIACRYCNYGVGNKYLREMAAHSTVPIMNLQCDLYHPMQGLADLMTIQEVFPNTKHL
                     KVSIIWAYAESHKKPISVPLTQILLFPRYAMDVTLAYPEGYDLPDWAVEKAKKNALEN
                     GGSFRITHDMKEAFKDADIVIPKNWGSWVKNQSTVVVDDYIKTLKGWKCTEEMMKLAS
                     PNVKYMHALPADRGNEVEDSVIDGPHSIVYQEAENRLHTAKAVMAMVMGDK"
     gene            complement(6254..7471)
                     /locus_tag="PLH17_01425"
     CDS             complement(6254..7471)
                     /locus_tag="PLH17_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017408345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOR20475.1"
                     /translation="MSKNIPFSEILKKAESYKKEMTAFLRDMIALPSESCQEKEVIHR
                     IKQEMEKVGFDRIVIDKMGNVLGYIGHGKHLIGMDAHIDTVGIGDIRNWTFDPYKGYE
                     DDVHIGGRGASDQEGGMASMVYAGKIIKDLGLEDDYTLVVTGTVQEEDCDGIPWQYII
                     EEDKVRPEFVVITEPTSCRIYRGHRGRMEIKVTTRGISCHGSAPERGDNAIYKMGPII
                     GEIKQLNERLKFDPFLGKGTMTISEIFHTSPSRCAVADSCWISIDRRLTMGETHLGAL
                     DEIRALPSVKESGATVEMYMYERPSWTGLVYPTESYFPTWVIPADHVICTSSVDAYKG
                     LFKKDPVVDKWTFSTNGVTIMGRYGIPCIGFGPGDEKEAHAPNEKTRKEDLVIAAALY
                     AAIPLMYVSKLKK"
     gene            complement(7468..8835)
                     /locus_tag="PLH17_01430"
     CDS             complement(7468..8835)
                     /locus_tag="PLH17_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOR20476.1"
                     /translation="MTKIPFGPTYDEMLHPDRINPEVRAKALKAKYADELDPINLFNI
                     NWKDDHGQVRKIVLPKELTGVEANIVVLLGKQFPSGSHKVGPAYSTLIEGCIDGDIVP
                     GQHTILGPSTGNFGIGVSYIANLMKYQAIVIMPDNMSKERYDRIQRYGAKLDLTPGSE
                     SDVILTLERTYELKKNPNYRTLAQFELMPNYRFHRHVTGNSCVEAVKDVGNGRIACFA
                     SAPGSAGTLAAGDGIKAVFPECKIAALEPYECSTLTNGGRGQHRIEGIGDKMCTLIHN
                     ILTTDFIALIKDDDSVRGLKIIHDAPQVLVRHGVDKKVAEEMRELFGVSGICNILGAI
                     KMAKHLRLGPGDNVVTIATDGYDRYESVLELLEKRELETADYVLERWFKDIFLGAEDA
                     NIEDARLPVVKERLFKQKEKDWLKFGYTQEYLDSMRHAEFWENEYHKVNEYDEKIKKM
                     RGKEL"
     gene            complement(8838..>9752)
                     /gene="thrC"
                     /locus_tag="PLH17_01435"
     CDS             complement(8838..>9752)
                     /gene="thrC"
                     /locus_tag="PLH17_01435"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOR20477.1"
                     /translation="GSTKDRASGVAVLKAIEAGAKIISCSSTGNAASSCACQAAHMGL
                     KSVIFVPKRAPIGKLTQLSIYGATVICVDGDYKDTYNLSRKAIETHHWYNRNAAINPH
                     MVEGKKTVALEIAEQLNFEPTDWVAISVGDGCSVGGVYKGFYDFYQLGLIKRIPKILG
                     VQSTGCAPFVTAFENDEPLKEADENTLADSIAVGIPRNPNKGLNAIKKSGGHFISVSD
                     EHILESMALLGRLEGIFGEPAGVTALAGVEEALRRGLIKPDESVTFIVTGNGLKDPTN
                     AQKAISQPVLLKPDIEALNKYLKEKGVK"
     assembly_gap    9753..9852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9908..10756)
                     /locus_tag="PLH17_01440"
     CDS             complement(9908..10756)
                     /locus_tag="PLH17_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOR20478.1"
                     /translation="MVRHIIPNNLNEALNFLNEGPFHIMAGGTDLMIQKRSTAGTPPK
                     FAKDVLYIANLTELNYIKKDEAHRCIRIGAAAKLEDIYNHVDTPALLKDIIREIGSPG
                     IRHSATLGGNIANASPAGDSLVALYLFDAEIVLLSVDGERIVPIKKFITGVRRIDLRS
                     NELIKEIVIPIIPITAYKWYKVGGRRADSISKVSFAGVYLSNFGLVADIRFAFGSVSA
                     TVARSIELENSLKNRPIEQIIGDLPRILEEADKMISPIDDQRSNKIYRRKVAGNIMER
                     FIKNIA"
     gene            complement(10750..11223)
                     /locus_tag="PLH17_01445"
     CDS             complement(10750..11223)
                     /locus_tag="PLH17_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOR20479.1"
                     /translation="MNSIKFNLNGQDVAVELNPSLRLLDVLRDHFHLTGTKEGCGEGE
                     CGACSVLINGRVVNSCCFPLANANGKKILTIEGLAATKRYQILKDAFHIEGGSQCGIC
                     TPGMIMAAESLLSMNPHPTDEEIRIGLSGNLCRCTGYNMIIKAVKTAAKLGEGLW"
     gene            complement(11216..13309)
                     /locus_tag="PLH17_01450"
     CDS             complement(11216..13309)
                     /locus_tag="PLH17_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOR20480.1"
                     /translation="MFDPNKSTPKIDHEEKCSGQAVYVSDITFDDILYAKTVRSTIAK
                     GYIRAIGVPTLPCGYHIVDHRDVVKENYVKMILTDWPVFAVDRVNYIGEPILLVVGPD
                     KRVIDEIVSAIQIDYEEETPVFDFVNSVVHKSFAKGDYSQAAKAAKRTIIETFNTGYQ
                     EQAYIEPQGMLAYLEEGGKVTLVGSMQCPYYIKNAVINTLGYPEDKVRVIQPAVGGAF
                     GGKEEFPSTIACQLATAVVKIKRPIKMIFEREEDMLVTTKRHPSEITLEAKIDENNKV
                     LGIKAHVSLDAGAYIGLSGVVLSRAMLAVTNAYTINNLDVSGDVYVTNTVPTGAFRGF
                     GSPQMIFAIEMFMNHLAKDLGLDPYQFRLNHLVRQGDLTSTSGLYRDPIILKDMIGKA
                     MAISDYQKKTEEYAKLDSFKGIGMSWFLHGCGFTGDGESAHIKAKVRIVKDEKDVVHL
                     HIAAVDMGQGARTSLKRVVAMTLGVENEQVVFDYPDTDLAPDSGPTVASRTMMIVGGL
                     LARAAQNLKDSWKAGERQEIIENYQPPQYIKWDESKLQGDAYPAYSWGVNIVEVEVSP
                     VTYEVELKGIWSVYDVGKAIDERIVIGQADGGITQGVAYGYLEVMRHENGKLRQKNLT
                     DYIIPTSMDMPPMETIIMDNPFAYGPYGAKGAGELTLVGGAPAVALAIENAIKRRVNR
                     IPATPEHIMELLNNE"
     gene            complement(13313..14401)
                     /locus_tag="PLH17_01455"
     CDS             complement(13313..14401)
                     /locus_tag="PLH17_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOR20481.1"
                     /translation="MDLSTKFDGLVLANPLMPAAGPLVGDSERLIFMKEAGCGAIVTK
                     TISTKDAHIPRPCIYGDREYIMNSELWSEYNKEKWIEEFLPEFVSKNDGRPLIVSVGY
                     TKEDMEVLIPLLDRFADAFEVSTHYVGTDHSVIAETVRTIRKHTKKPLYMKISPHIPD
                     PSGFARAIKEAGASGVVAVNSLGPTMKIDIKKRRIIYANEKGFVWTSGPVIKNLALAT
                     VYTIKQAEPSLTVIGVGGIASADDVIEFLLAGASGVQMLSAALLKGKQLYAKIIEDLP
                     KALERNGFTSIQEVIDTQLTNKVRYEGNTPYLIPEKCIRCMLCVKICPYFAIEMKDVI
                     TFDSTKCFQCGLCPVKCPTGAIVMEEKE"
     gene            complement(14401..15705)
                     /locus_tag="PLH17_01460"
     CDS             complement(14401..15705)
                     /locus_tag="PLH17_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR20482.1"
                     /translation="MKKAIINAKIYDFATFLDNGFVIFDHVINQVGAMENFVDEGYEI
                     IDARGALLMPGLVCAHTHIYSKFARGLSLPFNPKNFQEILDQMWWKLDRHIDNKASYY
                     SGIVAAADFLRDGVTTIIDHHASGKEIAGSLASLKKAVCDEASVRALFAFEVSDRFDV
                     NQAIQENVAFIKNFKTPFTAGLFGLHASMSLSEASLEKVRANLNGAPIHIHVAESEMD
                     EEDAQSKYHESVVERLDRHGLINEDSLLIHCVHVSEHELDIIEKRKAAIVVNVTSNMN
                     NAVGLPDINKFRDKGIPVMIGNDGLSSGAANEYINLLYGMHLLYKKPTAFGLGELLEM
                     IDSGYDYVSRRLGIKLGRIESGYEADLLLVPYMAPTAMDQSNALGHIFFGMMHAFKPS
                     DVFVAGEQLVKNYELTNKKLVMEYERSQEVANRLWQRIKGEK"
     gene            complement(15702..17009)
                     /locus_tag="PLH17_01465"
     CDS             complement(15702..17009)
                     /locus_tag="PLH17_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOR20483.1"
                     /translation="MYDYAIMNGKLYINGHWETHNLYISEGKIALISDDFLDAANVTD
                     ADGLEVLPGIIDPHVHFELDLGKYKSVDDFYHGTVAAAYGGVTSIVDFLDPAGNADEL
                     QKAFDKRMALAKNSVVDFRLHACIKNPSGDLEEFVKKMKSLDMHTLKLFTTYSDSGRR
                     TYEKDIIKLLELSEKYKFLLLAHIENDELIRLDKDFTYQDLPISRSSEAERSEALKLA
                     QLVRDHGGKLYMVHLSDGHTLEALIENYGDILNRRFFVESCPQYFTFTNDDLQREDGY
                     LYTFAPPLRSFVSRKLLFVNQKHIDTIGTDHCAFNVADKKGKLLFEMPLGIGGIEHSF
                     TIMRHHLGDAVINKMTVNIVRIQGLEGKGEIKVGNDADLVFFKPAPRSLILDQHGRAD
                     YSLYIGQPSHGQVISTMVRGQFVIENRVYQGGRGQWIKGRDIE"
     gene            complement(17014..17499)
                     /locus_tag="PLH17_01470"
     CDS             complement(17014..17499)
                     /locus_tag="PLH17_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOR20484.1"
                     /translation="MIKIITGKVNAGKTTTLLKTYRLDRKGDGFAAIKKMNGNEVYGY
                     NMLHLSNDNIIPWMVHQKYFLRDFTKTGKFGPFYLNLDLLAMLESIIDELIAQRISPI
                     YLDEVGVLEINGGGYHNILKKLLSSGLDLVITVRDDLVKPVASHFDINDYELVQVQGE
                     E"
     gene            complement(17554..18243)
                     /locus_tag="PLH17_01475"
     CDS             complement(17554..18243)
                     /locus_tag="PLH17_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20485.1"
                     /translation="MNNKKTLKNTVMAVIFFGAVWGLLEATMGYLLHWLPGMWSGLVM
                     FPIGSALMYWAYQNTGRRSAILYVGLLAAAIKAINFAFPIHWEGLMRVYTPMISIILE
                     SLTVFAVSYLFEKKASPVNQTLIHLGAIVLAIVSWRTLFLANQAVQSAISGNLAAQLS
                     SFEASARFVFVNGAYELLVFAIIYIIYRSIQALLAMRKEPLKTPNWLMYVLSPLTFVF
                     AVLAVVIPNLL"
     gene            complement(18256..20562)
                     /locus_tag="PLH17_01480"
     CDS             complement(18256..20562)
                     /locus_tag="PLH17_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOR20486.1"
                     /translation="MKTKVVNHKTPSVHGKGIMMGRPSFTDDLAEPNSLIVKILRSPH
                     AFARIKSIDVSKAKALTGVELVLTHHDVPRKSFTRAGQGYPEPSPHDKFILDEYVRYV
                     GDEVACVAAINESIANQALKLIEVEYEILEPVLDFETAFEHKSIIHPEDGINEMFPIG
                     FEPKKNVAASYHMHVGDVEATLAKCDVVIDETFYTQAQAHAMLEPHTANARLDYLNRL
                     VIYTATQTPYHVRRIISQTLDLPISKIRVIKPRVGGGFGGKQALHGEFFVSLVTLKTK
                     KPAKIIFSRQEVFESSYTRHPMRIRMRLGANKDGTLKAIDCYSLSDTGAYGEHALTVF
                     MITGSKVLPLYNKVDAVRFGGRVVYTNHVSAGAYRGYGAIQGNFALESAIDILAKKLN
                     IDPLELRRKNMMGEGETSPIFSIMGEGTEGTAMDMDTCKLGYCLDRGAELIGWKDKYP
                     FVQVGPHKVRSVGMAIAMQGSGIPYIDMAAATIKLNDDGFYNLTVGATEIGQGSDTVI
                     TQIVAEALMTTVDKVIIYSSDTDLTPFDVGAYASSTTYVSGNAALKAALKMKKMLIEE
                     AAQYLNAKPEEVMFDGKEFRTEDKRLTLADMANKLYYNENQHQLSATESFVGHKSPPP
                     FMAGYVEIELDTETGEYDILHYVSVVDCGVTINPMLAKGQVEGGIVQGLGMACFEDVK
                     YSGRGKLNSNSFLYYKIPSMLDVKKLTTEFAESYEESGPYGAKSVGEIGIDTPPAAIA
                     NAVANATGVRINKLPVTPEKIFQGLNKK"
     gene            complement(20559..21011)
                     /locus_tag="PLH17_01485"
     CDS             complement(20559..21011)
                     /locus_tag="PLH17_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOR20487.1"
                     /translation="MKVSFVLNGRNISYEIAPGEYLADTLRANHILSVKKACDEAACG
                     ACTVLLDGRPILSCSFLSARVEGRSVTTVEAVQDEVSEISDFFSDEGADQCGFCNTGL
                     ALTILALRNELKNPTDEEIKHFVVGNLCRCTGYQAQFKAIKRYLEAHK"
     gene            complement(21011..21805)
                     /locus_tag="PLH17_01490"
     CDS             complement(21011..21805)
                     /locus_tag="PLH17_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOR20488.1"
                     /translation="MEANRYLRPATLEEAHQLLLQDAKNILLGGGLWLKKSTSSANAL
                     IDLSALGLDQIKNTGDHIEVGAMVSLRDFEMSPLVKNIQGGILVSAVSQIMGVAFRNG
                     ATVGGSVAGKFPFSDLITPLLALDTTLIFYPKKELGLADFLNQKGRSSDILTHVVIKK
                     STGRAFFKKVKTTALDFAVINVAISRLNNEYRIAIGSRPSVAALAVKAMDTVNGMKAL
                     SDEQIAQAATLASEELLYASTNAASAEYRKALVRAYIKRGLEEVSK"
     gene            complement(21822..22409)
                     /locus_tag="PLH17_01495"
     CDS             complement(21822..22409)
                     /locus_tag="PLH17_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOR20489.1"
                     /translation="MGRTDIKQGVNLMTQGIILAAGYSSRAETNKMLLVYKGKFLLAH
                     AVEGMMPFVDEIIVVTGHFHETIKEVVKPYAKVTIVYNGDYAQGMFSSVQAGVRKTNG
                     DFFVLPGDCPFVTADTYRKMLAGSKEIRVPAYRGQTGHPLFLKHSLRDELLNEPVTSN
                     LKVFRNRHDYEIIKTEDLQILMDIDTLHDYQNLDK"
     gene            22704..23624
                     /gene="arcC"
                     /locus_tag="PLH17_01500"
     CDS             22704..23624
                     /gene="arcC"
                     /locus_tag="PLH17_01500"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOR20490.1"
                     /translation="MSRYVVSLGGNALGNNAEEQKKLLINVAEPIVALIKAGHEVIIV
                     HGNGPQVGMINLAFAESKSTPDMPFPECGAMSQGYIGFHLQNAIYNELAKQGIAKHVA
                     TIVTQVLVDEHDPLFQNPTKPVGAFYSKEEADIIAQEKGYVMKEDSGRGYRRVVASPI
                     PVDVVEKEMILSLIKDQHVVICAGGGGIPVIYKGSHLEGIAAVIDKDYASAKVAALVD
                     ADYLVILTAVDNAYINYRKPGEKRLELITMAEIEKYSEEGYFAKGSMYPKVQACLMFL
                     KAKTGKTAIISSLEKAKEAFLEKTGTIIKS"
     gene            complement(23928..25109)
                     /locus_tag="PLH17_01505"
     CDS             complement(23928..25109)
                     /locus_tag="PLH17_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOR20491.1"
                     /translation="MKKVLCLNKISPVGLKTLPHNYEVTSEINEADAILVRSAVMHEM
                     ILPDNVVAVARAGAGVNNIPLDAYAKAGVVVFNTPGANANAVKELTIAGMLLAARDIH
                     GGMKWVEANKDDVEINKNMEKAKAAFAGTELLGKTIGIFGLGAVGTLVAQGAYGMGMS
                     VAAYEPSQATIDKNRDLFPAGLRLVASADELYTAADYISLNVPLLPATKGMINRESIA
                     KMKDGVVIINLARDAIVNDDDIRQALKEGKVRRYVTDFPNHATANMEGVVAIPHLGAS
                     TEEAEDNCATMAVKQIVDFIENGNIVNSVNYPNIELGPKTEAHRIVLLHDANEGLSGQ
                     IIKIVETRGVVKQIASKVKGAFGVTLVDFVKTKGECSEGVCLGDQLGELPGIISLRVI
                     H"
     gene            complement(25118..26203)
                     /gene="serC"
                     /locus_tag="PLH17_01510"
     CDS             complement(25118..26203)
                     /gene="serC"
                     /locus_tag="PLH17_01510"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOR20492.1"
                     /translation="MKRIYNFSAGPAVLPEEVLQQAADEMLDYKGSGMSVMEMSHRSK
                     TYQAIIDEAEADLRKVLNIPGNYKVLFVQGGGNVQFAMVPMNLSKNRIYDFILTGHWS
                     KAAKKEAEIFGKVNVIASSEDKNFSYIPNLDNVKASPDSDFVCMCDNNTIFGTKYKKY
                     PEFPGKILVNDASSSFLSEPMDVTKFGLIYAGAQKNVGPAGVVIVIIREDLLENEPLP
                     GTPTLLKYKVLADAGSMYNTPPTYGIYICGLVFKWILAHGGLEAMKERNEKKAALIYD
                     FLDNSKIFKGTVDKGSRSLMNVCFRTDSEELNEAFLEGAKKAGFANLKGHRSVGGMRA
                     SIYNAMPLAGVEALVKFMREFEAAHVK"
     gene            complement(26223..27443)
                     /locus_tag="PLH17_01515"
     CDS             complement(26223..27443)
                     /locus_tag="PLH17_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOR20493.1"
                     /translation="MKIVNPIRPPHILLPKDGVDMNAFAVIACDQFTSQCDYWDGLKE
                     KIGDKPSTFHMIFPEAYLTLVNEKDYITGINRNIDRYLKEDVLKDIGECFVLVERNTP
                     HTKRRLGLVISVDLEAYSYEKGTKALIRATEATIVERIPPRLKIRENAPVELTHTLFL
                     FDDPKKSIIEKIYEKRAEMPLLYDFDLNQNGGHLRGYQVKDTQKVIDAFTKLTEENGK
                     DLMFVVGDGNHSLATAKAHWDRLKIGLSAKERETHPARFALVEAINLYDEGLDFEPIH
                     RVVFEVKSDFVPGLRKVVDGTHETHVYCKPKGQSPLSVPANAPLAYRLIQDYIDTYVK
                     NTPGSSVDYIHDIDYTHEVADKHPTSVAVAMPALTKADLFDYIAKGDVLPRKTFSMGH
                     AVEKRYYLECKRIK"
     gene            complement(27446..28792)
                     /gene="gdhA"
                     /locus_tag="PLH17_01520"
     CDS             complement(27446..28792)
                     /gene="gdhA"
                     /locus_tag="PLH17_01520"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOR20494.1"
                     /translation="MYQSSYLNKLFEDVKKKYSSEPEFISAVEEFFDSMDYMVDDEPN
                     IEKYAILERILIPERVISFRVAWVDDKGKVQVNKGWRVQFNSAIGPYKGGIRFDSSVN
                     LSILKFLAFEQTFKNSLTGLPLGGGKGGADFTPRGRSDEEIMRFCQAYVSELFRHIGP
                     DTDVPAGDFGVGAREIGYMFGMYKKLSNDFVGTFTGKGISYGGSLGRKEATGYGLLYF
                     VSEMLKTFHDDTLKGKRVIISGSGKVGKHAAVKAHALGAKVVAMSDINGFVYDENGLD
                     VPLIDKLSEPKGEYSLEYMKIYPEAKFVPTPKLMWGMPCDIALPCATQNEIDLEDAKK
                     LKANGCYMLVEGANMPTAVEAIRFCNENGILFAPGKASNAGGVAVSGLEMSQNAMHLS
                     WTYEEVDQKLEQIMKNIFRKCHETAQKYGQPKNLALGANIAGFIKVYDAMLQEGVI"
     gene            complement(28914..>29130)
                     /locus_tag="PLH17_01525"
     CDS             complement(28914..>29130)
                     /locus_tag="PLH17_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20495.1"
                     /translation="NPVYFILGFDIRKLVNKIYRIEVVLFACSDAESLQLASIIPSAV
                     AFLLWSLQTENDRRFKESERHSFDLDK"
BASE COUNT         7438 a         6685 c         7182 g         7725 t
ORIGIN      
        1 cgagatataa gtagatgctc gtcgtcaaag taaagccctc agccagcaag gcgataacta
       61 gttcgtaaac gaaaccggcg aagatggcga gtaaaacaaa cggccagtat ttattaaaga
      121 aatggacatg ggggtaatga gtctcaaact tctcgagcaa gaaaatcatg gctaaggtca
      181 agaaggcggt gaccaaggcc gatatcagat acacgaaacc atagtaattc ggatttacaa
      241 aggaagtaat tagatagacg attaaaatcg ccgagaaaat catgattaaa ggcacgacga
      301 tgaaggcaaa gaccgtcttc cagatgacat ggtaatcgct ttcttgctta tcttcgctgt
      361 ggctaggtaa aaagccaatg aagaccggaa cagccacgaa gccgatgacg gcaaaaaaca
      421 aatcaatgta gataaattcg ctgaggttga ttccgaacaa tgcttcaatg ctcgccaaaa
      481 tggcaaaaat gccgccatat aagacagcgg catagaagat ggtcacgaaa aatttcgtga
      541 gtaaaaagac tacaaaaaag gcgaagtttt cgcgcttggg aaaatagttg acgatgagag
      601 cgagcaagac ggcgatagcc caggccgccg ccaaacgaat gcttaaaatt acatcggttg
      661 tcgtaagcca gaagcgggtc aacaaaaaga aggaaaccgt cacgatgagg gcaccaccgg
      721 taagaagata tttccaaagg actttaagac cgattctttc agcgagaaga gtcttaaaaa
      781 ttaagaaggg cagggtcagc cataaaaagg gcagcatctc gccaaaaaag gccagtccgc
      841 cccgcgtttc gagattgacg atgctaaaaa taacgagaag aatgctcaat ataattgagt
      901 cggtaaacct ttgaaagctg cgaacaaacg gatcgagaag cgacgttttt aaaaatatcg
      961 caaatttatt catttgttcc cccttgtgcc gcctccgcta ggaagggcga aaactagctg
     1021 ccgcggcggc tggttatatt tgacctttgc ggcttctgtt ttactcttgt gttctgacgg
     1081 cgaatctgat tgccgctcgg cccttgcatg aagggcggga cttttgcttt aacgcctttt
     1141 gccataaatc cacctctttt ttctaatttt ataatagcac atcaaaaaac aaacaattcg
     1201 tattcacatt atctttatgc ttctattttc tgcaaacgaa gggaagctgt tttgtgcatc
     1261 ggccagtaga tgaggaaagc tagtaaacca ttgataattg tcagaaatag aatcggaaaa
     1321 gcaagcggaa tgggaatcat gattaagccg aagagaattg ccccctccag cgcgatgatg
     1381 ccaaagccga cgaagacggc gatgaaagcc gacatgcttt gcttgacgat ttccgcttcg
     1441 cttttaaagt caaagcgcgg gaaaaagaga ttgagccagg caaagaatat cgaggtgaga
     1501 accgcaaatg agataacggc gagaaaactc gccagtgaag tccataaatc aaggccgagg
     1561 ccgatggcgg caaaggggaa agtgaagatg atgaccggcg cttcaatcaa tagattgaag
     1621 atgattttgg cgctcatgat cgtctcgcct ttaatcggca gtgtttttag aagaccaaaa
     1681 ttattgcctt ccagcgagag gctaatcgcc ggagtataaa ccgtcgacag gcaaaaagca
     1741 taggcgatta agaggacgaa gggagcgaat aatccgagcg aagccgccaa ctcattttta
     1801 aaaaatatgg ccgccaccgc catgatggca agcatgatta agccgaatcc ggtgttaaaa
     1861 atgtagatgg gtgtaccgac aaattttcgc cactcttttt taatgagagc gatgaaaatc
     1921 ggcgtctgct gacgcttatt gctcttgata gtaacttgcc tgttggcagt ccggttctga
     1981 ttggtcttaa cactcaattt ggagatagcg ataagaaaga tgacgaaaat accagcatga
     2041 ctcaagaaca ggtaaacaaa gtttaaaaga ttgttttcgt ggacggcgga agcataccat
     2101 tcattcggca ggtagaaact cgatatcgca tcgatgatcc aaacgggaat aaacgagcct
     2161 tcaccgctgc ttgcactgct gataagcgag aagccaaaga tggtgagaaa aaggacgaac
     2221 atcaaaatgg tttgaatgag gttcgatttg gcaaagcgct tggaaatctt ggcgattaaa
     2281 agcgataaaa aggagccgag aataatcgag ggcaagggta agagcaagta accgaacagc
     2341 aagaaaataa aacccaaaag gtcaaatggc accaggatgt aataaacgat aaaaagaggc
     2401 aaggtcaaag ccgtcagcac aagatagata aaaaccatca tcgtcttcag tttggccaga
     2461 agaagagaaa tcggttttat cggaaggggt ccgagaatct cgtaatcttt aaactgaaaa
     2521 agaaagccgt tggcctgaag gagggcaaac aagaagccca atcccgaggc ataagaggcg
     2581 acatagagca ttaactcgcg cttcagcccc attgaataaa acatcatgcc ggtcgaaaaa
     2641 ccgatgctgg caaaggagta gacaaaaagg agggcgaagg cgatgatgcg cccttttgat
     2701 ttggcgactt tgcttccgaa aaggcggcgc gcgccaaagt tttcttttag aaaaaccttc
     2761 agcaatttaa aaaacacgtt aagcgatctc cataaagaag ttctcgagcg aacgatcttt
     2821 taagacttca tgggtcttgc cgtcggcgat aatctcgcct tgtttgataa tggcaatttt
     2881 gttgcacagt ttttcaacga cttccaggac gtgcgaagaa aagaggataa tcgtgcctgc
     2941 atcggtaatc tctttaaagg tcttttttaa ggcgaaggcg gctttcggat caaggccgac
     3001 aaacggctcg tctaacaaga taagtttcgg ctcgtggaca agggcggcca tcaaagctgt
     3061 tttttgcttc atgccgtgcg agtaggagga aatcggttta tcaagagaat cgagcatctc
     3121 gaattccgcg gcgtatttgc cgatgagctg attgcggcgt tctaaagaga cgttaaaagc
     3181 atcggcgata aaatcgatga actgccggcc cgacaaggtc tcatataaat cggggtggtc
     3241 gggaatgtag gctaattgct ttttaaactc gagaggttcc ttggcgatgg tgtgaccatc
     3301 gaaagtgatg gtgccgctat cgaagctgat gataccggca atcgatttga tcagcgtcgt
     3361 tttaccagct ccgtttggac cgacaaaacc atacaaatcg cccggctcta aatgcatgga
     3421 aatattattg atggctaatt tctttttatc atatgatttg ctgacgtttt tgatatttaa
     3481 cattggtgga tccccctaaa aacgaacaat attatattag caaaaatgtg attgtattta
     3541 tacattatat aaataaatag accgacaatt tgttttgccg gtcagttaac ttgtcttaat
     3601 agttgttttt gatttctaga tacatcgcat atttggcgag gttgactttc ggctcgaccc
     3661 agaaggtgag gatgccaaaa gtcaagagga cgaggatgta ccagacgatg tggctcagat
     3721 acaagcagaa gagccgccac ttattgccgt tcataatctt gcggctgcgc atgatgacat
     3781 cttcataatg catatcagga ttatcgagtt gaataaagta aatcatcgag taggagaagg
     3841 atttgatgat gccgggaata agaaagagca tcgaccaaag gagtagatat aactggctga
     3901 tgagtccgag caaaatgcgg tcgccgactc tttcgtcaaa acctttgacc aaggtgccga
     3961 tattcggtct ttcgccacga tataaatcag cgataaacat cgcataaccg atattaatcg
     4021 gaccgccgat taagaacatg ccaataccac cgatggaagt gacaaagcca accaaaagac
     4081 tataaagaaa ttgcactaag gccgcttcgc tccacttgcc gcttaatttt tcatcggcga
     4141 ttcttcgaaa ttcaaataat gacataatga tcacccccgg taattatatt aaacaatcga
     4201 aatattatta agcaaaaaaa tacgacgagc gccgtttttt gcgtgaaatc cgttaattgt
     4261 tttaaagggg gcaagccacg tctttaactt cttggagggt ggcgtcgctc atcaagacat
     4321 cggcataaac cttcgacatt tccatgatat ctttggcaaa aggacaggtc ggaatgattt
     4381 taaggttgtt ttcgcgagca taggaaacga cgcgatcgac aagaatttta cccattccgc
     4441 gccctcttaa cgaacgattg acataagtat gatctaagat aataatatta tctttatgtt
     4501 ttttaaaagt cacctcagcg agggctttat cttcgctttc tcccatgtag aagcggtctt
     4561 tgttttttcc aaaaattagt tgttccattt ttttatcctc cgaataatta tctatgtcag
     4621 tcaaaaatga ctatttaact tactatattt tagcgcttca aatgatagta gggccaatat
     4681 tatgctttat taagtgagga aaatgaattg aggctccgac ttaaataacg gtgtgatgaa
     4741 tgccaaattt actggtcaag aaaagtaact cctttagtca aaaccagcgt gagtcggacc
     4801 tcggcttttt caccgaccaa gcgaatgcgg ttaaagccct tttcgagttt aaaaatacgc
     4861 acttctctgg ccgtgctcac ggcgatatcg cgaatcgatt gctttctaat taaaacgact
     4921 ttcgcgcgtc cgctgagaac ttcaaaatca acatcgatgc ggcacgaggt attttcgcgc
     4981 aagtaaaaaa ggccgattgt cttcactccg gtaaaagacg tggcgtgcat tttaatatta
     5041 ttgccgaact ggcggccgac catcaccagg gctcggacgt tattgacgcg gcttagtatg
     5101 cgccgctcgt cattaaaaat atccatacaa ttcccccgta aatattgtaa acctcgcgta
     5161 ttttgaaaaa aagatataaa aagggactgc tctctttttt tgagcaatcc cttaacaaaa
     5221 gtagatttgt tttacttatc gcccatcacc atcgccatga cggctttggc ggtgtggagg
     5281 cggttttccg cttcctgata gacgatggag tgcgggccat cgatgaccga atcttcgact
     5341 tcgttgccgc ggtccgccgg aagggcgtgc atgtatttga cattcgggct ggcgagcttc
     5401 atcatctctt cggtgcactt ccagcctttt aaggttttga tataatcgtc gacgacgacc
     5461 gtggactggt ttttaaccca gctgccccag ttcttcggaa tgacgatatc ggcgtcttta
     5521 aaagcttctt tcatatcgtg ggtgatgcgg aaggagccac cattttcaag ggcgttcttt
     5581 tttgctttct caacggccca gtcagggagg tcataacctt ccggataggc gagggtgaca
     5641 tccatcgcat agcgcgggaa gagcagaatt tgggtgagcg ggacgctgat gggcttttta
     5701 tggctttcgg cataagccca gataatcgat actttcagat gctttgtgtt cggaaatact
     5761 tcctgaatgg tcattaaatc cgcgaggccc tgcatcgggt gatagaggtc acactgcaaa
     5821 ttcatgatcg gcacggttga gtgcgcggcc atctcgcgaa gatatttatt gccgacgcca
     5881 taattgcagt agcggcaggc aatgccatgt ccaaacgagg acaagatgac ggcggtatct
     5941 ttggcgcttt cgccatggga aatctgcatc atcgaggtgt cgaggaacgt cgcgtgaccg
     6001 cctaattggg cgagtccgga ttccatcgag tttctcgtcc ttgtcgattg ttcgaagaac
     6061 atcaagaagc cggttttatt ctcgagatag ggggtcggaa tattcttgta gaatttgttt
     6121 tttaaatcgt aagagacttc cagtagctta tcgatttctt ctttagtcca ttcttcaagg
     6181 gtgatgaagt ggcggccttt gaagagtggt ttcatcgttt ttttgtaaaa cagtttgctc
     6241 attcgttttc tccttatttt ttgagtttgc tgacgtacat taaaggaatg gcggcataga
     6301 gcgcggcagc gatgacgagg tcttcttttc tcgtcttttc attgggcgcg tgtgcctctt
     6361 tttcgtcacc gggcccaaag ccgatgcagg gaatgccata acgtcccata atggttacac
     6421 cgttcgtcga gaaggtccat ttgtcaacga ccgggtcttt tttaaacaag cctttatagg
     6481 catcgaccga ggacgtacag atgacatggt cggcgggaat aacccacgtc gggaaatagg
     6541 actcggttgg atatactaag cccgtccaag aaggacgttc atacatgtac atttcaacgg
     6601 tggcaccaga ctctttaacc gagggtaaag ctctgatttc gtcaagcgcg cctaaatgcg
     6661 tctcgcccat cgttaaacgg cggtcaatcg agatccagca actgtcggcc accgcgcaac
     6721 gcgaaggcga ggtatggaaa atctcgctga tcgtcatcgt gcctttgccg aggaatggat
     6781 caaattttaa ccgctcattg agttgcttga tttcgccgat gatcggcccc atcttataga
     6841 tggcgttgtc gcctctttca ggggcgctgc catggcagga gatgccgcgc gtcgtcactt
     6901 tgatttccat cctaccgcga tggccgcggt aaatgcggca cgaagtcggt tcggtgatga
     6961 cgacgaattc ggggcgaact ttgtcttcct caatgatata ttgccacggg attccgtcgc
     7021 agtcttcttc ctgcacggta cccgtcacaa ccaacgtgta gtcgtcttca agacctaggt
     7081 ctttaatgat tttgccggcg tagaccatcg aggccatgcc gccttcctga tcgcttgcgc
     7141 cacgaccgcc gatgtgaaca tcgtcttcgt agcctttata cggatcaaaa gtccagttgc
     7201 gaatgtcgcc gatgccgacg gtgtcgatat gggcatccat gccgatcaga tgtttgccgt
     7261 gtccaatata gcctaacaca ttgcccatct tatcgatgac gatgcggtca aagccaacct
     7321 tttccatctc ttgcttgatg cggtggatga cttctttttc ctggcacgac tcgctaggca
     7381 aggcgatcat atcgcggaga aaagcagtca tctccttttt gtaagattcg gcttttttca
     7441 gtatttcact gaagggaatg tttttgctca cagttctttt cctctcattt ttttaatctt
     7501 ttcatcgtat tcattgactt tgtgatattc gttttcccag aattccgcat gtctcatcga
     7561 atcaaggtac tcttgggtat agccaaattt aagccagtct ttttcctttt gtttaaagag
     7621 tcgctcttta acaaccggca gacgcgcatc ttcgatgttg gcatcttccg cgccaaggaa
     7681 gatatctttg aaccagcgtt ctaggacata atcggcggtt tcaagttcgc gcttctcgag
     7741 taattcgaga acgctttcgt agcggtcgta gccgtcggtg gcgattgtca cgacattatc
     7801 gccggggccg agtctcaggt gttttgccat tttaatggcg cctaagatat tgcagatgcc
     7861 ggatacgcca aatagttcac gcatttcctc ggcgactttt ttgtcgacgc cgtggcgcac
     7921 taagacttga ggtgcatcat gaatgatttt caaaccgcga accgaatcgt catccttaat
     7981 caaggcgatg aaatcagtcg tcagaatgtt gtgaatcagc gtgcacatct tgtcgccgat
     8041 gccttcgatg cgatgctgac cgcggccacc gttggtgagc gtcgagcatt cgtatggttc
     8101 aagagcggcg attttgcatt cggggaagac ggctttaatg ccatcgcccg cggctaatgt
     8161 accggcgctg ccaggagcag acgcgaaaca agcgatgcga ccattgccaa catctttgac
     8221 agcttcgacg caactatttc cggtgacgtg acggtgaaaa cgataattgg gcatcaattc
     8281 gaattgggca agagttcggt aattcggatt tttcttgagc tcataggttc tttctaatgt
     8341 caagatgaca tcagattctg aaccgggcgt cagatcgagt ttggcgccgt agcgctgaat
     8401 gcgatcataa cgctctttgg acatattgtc gggcatgatg acgatagcct gatatttcat
     8461 taaattggcg atgtaggaaa cgccgatgcc gaagttgccg gtcgatggtc ctaaaatcgt
     8521 gtgctgacca ggaacaatgt cgccatcgat acatccttca atcagggtcg aataggccgg
     8581 tcccacttta tgggatccag acggaaattg tttgcctaag agcacgacga tgttggcttc
     8641 gacgccggtc aattctttcg ggagaacaat tttgcggacc tggccgtgat catctttcca
     8701 gttgatgttg aaaagattga tgggatcaag ttcatccgcg tacttggcct ttagggcttt
     8761 cgcgcggact tccggattga tgcgatctgg gtgaagcatc tcatcataag taggtccaaa
     8821 tggaattttt gtcatgttta tttgactcct ttttctttca aatacttgtt cagggcctcg
     8881 atatcgggct taagaaggac gggttgacta attgcctttt gagcattcgt cgggtctttt
     8941 agaccgttgc cggtaacgat aaatgtcacc gattcatcag gctttatcaa acctctgcgc
     9001 agagcctctt cgacgccggc aagtgcagtc acgcccgctg gttctccgaa gataccttca
     9061 aggcgcccta aaagcgccat actctctagt atatgctcat cgctgacact tataaagtgc
     9121 cccccactct ttttgatggc atttagacct ttgttcgggt tgcggggaat gccgacggcg
     9181 atggaatcgg ccaatgtgtt ctcatcagcc tcctttaatg gctcatcatt ttcaaaagcc
     9241 gtcacaaacg gcgcgcaccc cgtgctttgc acaccaagaa tttttggaat gcgtttaata
     9301 agacctaact ggtaaaaatc ataaaagcct ttatagacgc cgccgacgct gcatccgtcg
     9361 ccgaccgaga tggcgaccca atcggtcggt tcaaaattaa gctgctcggc aatttcaaga
     9421 gccaccgttt ttttaccttc gaccatgtgc ggattaatcg cggcgttgcg attataccaa
     9481 tggtgtgtct cgattgcctt acgcgacagg ttataagtat ctttataatc gccatcgacg
     9541 cagatgacgg tcgcgccata gatcgacagt tgcgtcagct tgccgatcgg agctctcttg
     9601 ggaacaaaga taacgctttt gaggcccatg tgagccgcct ggcaggcgca actcgaagcg
     9661 gcattaccag tcgaggagca ggagatgatc ttcgcgccgg cttcaatcgc ctttaagacg
     9721 gcgacgccgc tggcccggtc cttggtggag ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nncttgccgc acagagtgca gcgataacct ttaaatttgt gcattatttc
     9901 accttcttta cgcaatgttt tttataaagc gttccataat gttaccggca actttacggc
     9961 ggtaaatctt attagaacgc tgatcatcaa tcggggaaat catcttgtcg gcttcctcta
    10021 aaatacgagg taaatcaccg attatttgct caattggacg gtttttaagc gaattttcga
    10081 gttcgatact acgcgccacg gtcgccgaaa cagagccgaa agcaaaccga atatccgcca
    10141 ctaaaccaaa gttggaaaga tagactccgg caaaggacac cttgctgatc gaatcggcgc
    10201 gccgacctcc gactttatac catttgtagg cggtaatcgg aattatgggt atgactattt
    10261 ctttgattaa ttcgttgctg cggagatcga tgcggcgcac gccggtaata aactttttaa
    10321 tcggcacaat ccgctcaccg tcgacactta ataaaacaat ttcggcatca aaaagataga
    10381 gggcgaccaa actgtcgcca gcgggtgagg cgttggcgat attgccgcct aatgtcgcgc
    10441 tgtggcgaat gccggggctg ccaatttcgc gaatgatatc ttttagtaga gccggggtat
    10501 cgacgtggtt ataaatatct tctaacttgg cggccgcacc aatgcggatg cagcggtggg
    10561 cttcgtcctt cttaatataa ttaagttcgg tgaggttagc gatataaaga acgtctttgg
    10621 cgaatttcgg gggcgtgccg gccgtgcttc ttttttgaat catcaaatca gtgccgccgg
    10681 ccatgatgtg gaagggtcct tcgtttaaaa aattgagggc ttcgttaaga ttattgggaa
    10741 tgatatgtct taccataatc cttctcctaa cttggcggcc gttttaaccg ccttgataat
    10801 catgttgtag ccggtgcaac ggcacaagtt tcccgacaac ccgatgcgaa tttcctcatc
    10861 ggtcggatgg gggttcatcg aaagcaaact ctcggcggcc ataatcatgc ccggcgtgca
    10921 tattccgcat tgcgaaccgc cttcgatgtg aaaggcatct tttaaaattt gatagcgctt
    10981 ggtggcggcc aaaccttcga tggtcaaaat ctttttgccg ttggcgttag ccagaggaaa
    11041 gcaacagctg ttgacgacgc ggccgttaat taagaccgag cacgctccgc attcgccttc
    11101 accgcaacct tctttcgtgc cggtcagatg aaaatggtcg cgaagaacat caagcaagcg
    11161 caagctcgga ttgagttcga cggcgacatc ctgtccattt aaattaaatt ttatgctatt
    11221 cattatttag taactccatg atgtgttcgg gcgtggcggg aatgcggttg acgcggcgct
    11281 tgatggcgtt ttcaatcgca agggccaccg ccggcgcgcc tccgacaagc gtcaattcac
    11341 cggcgccttt ggctccgtaa ggaccatagg caaacggatt atccatgatg atggtttcca
    11401 tcggcggcat atccattgac gtcggaatga tataatcggt taaattcttc tgcctcaact
    11461 tgccgttttc atggcgcatt acttcaagat agccataagc tacgccctgg gtgataccgc
    11521 catcggcttg gccgatgacg attctttcgt cgatggcttt gccgacatcg tagacgctcc
    11581 agatgccttt gagttcgacc tcataggtaa ccggcgagac ttcgacttcg acgatgttga
    11641 cgccccatga ataagcggga taggcatcgc cttgcagctt actttcatcc catttaatgt
    11701 attgtggagg ctgataattt tcgatgattt cctggcgttc gcccgctttc cagctgtctt
    11761 ttaagttttg ggcggcgcgg gctagaagtc caccgacaat catcattgtc cgcgaggcga
    11821 ccgtcggtcc tgagtcggga gccaggtcgg tatcgggata atcaaaaaca acttgctcgt
    11881 tttcgacacc taaagtcatc gccaccacgc gtttaagcga ggttctcgct ccttgaccca
    11941 tgtcgacggc ggcaatatgg agatggacga catctttttc gtctttgaca attcggactt
    12001 tagccttgat gtgcgcactt tcgccatcgc cagtaaagcc gcatccgtgt aaaaaccagc
    12061 tcatcccgat gcctttaaag gagtcgagtt tagcatattc ttccgtcttt ttctgatagt
    12121 cagaaatggc catcgctttg ccgatcatgt ctttgaggat gatcgggtcg cgataaagac
    12181 cggatgtcga agtcaagtcg ccttgcctaa ccaaatggtt aaggcggaac tggtacgggt
    12241 cgagacctaa atctttggcg aggtgattca taaacatttc gatggcaaag atcatctgcg
    12301 gcgaaccaaa cccgcgaaag gctcccgtcg gaacggtgtt cgtcacatag acatcgccgc
    12361 tgacatcgag gttatttatc gtataggcat tcgtgaccgc cagcatggcg cgcgagagca
    12421 cgacgccgct taagccgata tacgctccgg cgtcgaggct gacgtgggct ttaataccca
    12481 atactttatt gttttcatca atcttggctt caagagtgat ttcggaaggg tggcgcttcg
    12541 tcgtgacaag catgtcctct tcgcgttcaa aaatcatctt aatcggcctt ttgattttga
    12601 cgacagcagt cgcgagttgg caggcgatgg tcgaaggaaa ttcttctttt ccgccaaagg
    12661 cgcctcccac cgcgggctga atgacgcgga ctttatcttc gggatagccc aaggtattaa
    12721 tgacggcgtt tttaatgtag taaggacact gcatcgaacc gaccaaagtg actttaccgc
    12781 cttcttcaag ataagctagc atgccttgcg gttcgatgta ggcttgctcc tgataaccgg
    12841 tattaaaagt ttctataatc gtccgcttcg ccgcttttgc ggcctggctg taatcaccct
    12901 tcgcaaagga cttgtggacg acgctgttga caaagtcaaa aacgggcgtc tcttcttcat
    12961 agtcgatttg aatggcggag acgatttcat cgatgacgcg cttgtcggga ccgaccacga
    13021 gaagaatcgg ctcgccgata tagttgacgc gatcgacggc aaaaacgggc cagtcggtta
    13081 aaatcatttt gacatagttt tctttgacga catcgcggtg gtcgacgatg tgatagccac
    13141 agggaagggt tggcacgcca atcgcgcgaa tatatccttt ggcgattgtc gaacggactg
    13201 tcttggcata caaaatgtcg tcaaaggtga tgtccgagac atagacggcc tgaccagaac
    13261 atttttcttc gtggtcgatt ttcggtgttg atttattcgg atcgaacatt aattactcct
    13321 tttcttccat gacgatggct cccgtcgggc atttgacggg gcaaagaccg cactgaaagc
    13381 acttggttga atcaaaagtg atgacatctt tcatctcgat ggcaaagtat ggacagattt
    13441 tcacacacaa catacagcga atgcactttt ccggaatgag atagggggta ttaccttcat
    13501 atctgacttt gttagtgagc tgcgtatcaa ttacttcctg tattgaagtg aagccgtttc
    13561 tttcgagagc tttgggcaag tcctcgataa tcttggcata gagctgtttg ccttttaaga
    13621 gagcggcgga gagcatttgc acgccgcttg cgcccgccag caagaattcg atgacgtcat
    13681 ctgccgaggc gatgccgcca acgccgatga cggtcaatga cggctcggct tgtttaatcg
    13741 tgtacaccgt cgctaaagcg agatttttaa tgaccggccc gctcgtccac acaaagcctt
    13801 tttcgttggc gtagatgatg cgccgtttct taatatcaat tttcatcgtc ggacctaagg
    13861 agttgacggc gacgacgccg cttgcacccg cctctttaat cgccctggca aatcctgacg
    13921 gatcgggaat gtgggggctg atcttcatat agagaggttt tttggtgtgc ttgcgaattg
    13981 ttctgacggt ttcggcgatg acggaatggt cggtacccac atagtgggtt gacacctcaa
    14041 aggcatcggc aaaacggtcc aagagaggta ttaaaacttc catatcttct ttggtgtagc
    14101 caacgctgac aatgaggggg cggccatcat ttttggagac gaactcaggg agaaattctt
    14161 caatccattt ctctttgttg tattcgctcc ataattcgct gttcatgatg tattcgcggt
    14221 ctccgtaaat gcacgggcgc ggaatgtggg catctttggt cgaaattgtc tttgtgacaa
    14281 tcgctccgca gccggcctct ttcataaaga tgaggcgttc gctatcccca acgaggggtc
    14341 cggccgccgg catgagcgga ttagcgagca ctaatccatc aaactttgtc gataaatcca
    14401 tcatttctct cctttgattc tttgccatag tcgattggcg acttcttggc ttctttcgta
    14461 ttccatgacg agttttttgt tcgtcagttc gtaatttttg actaattgct caccggcgac
    14521 gaagacatcg ctcggcttaa aggcgtgcat catgccgaaa aagatgtgac cgagagcatt
    14581 gctttgatcc attgctgtcg gagccatata aggaacgaga agcagatcgg cttcgtagcc
    14641 gctttcgatt cgcccgagtt taatgccgag gcgacgggag acatagtcgt atccagaatc
    14701 gatcatctca aggagctcgc cgagtccaaa agcagtcggc ttcttataaa gaaggtgcat
    14761 accatataaa agatttatat attcgttggc ggctccgctg gagagcccat catttccgat
    14821 catcacggga atgcctttgt cccggaattt attgatgtcg ggaagaccga cggcattatt
    14881 catgttcgat gtgacattga cgacgatggc ggctttccgc ttttcgatga tgtcgagttc
    14941 gtgttcgctg acatgaacgc agtgaataag gagactgtct tcgttaatca ggccgtggcg
    15001 gtcgagccgt tcgacaacgc tttcgtgata tttgctctgc gcgtcttctt catccatttc
    15061 gctttcagcg acatgaatgt gaatcggagc gccgtttaaa ttcgcgcgca ctttttcaag
    15121 gcttgcttcg gagaggctca tcgaggcatg aaggccgaag aggccggcgg taaaaggagt
    15181 tttaaaattc ttgatgaagg cgacattttc ctgaatggct tgattaacat caaagcgatc
    15241 gctcacttca aaagcgaaca gagcgcggac agaagcttca tcgcacaccg ctttctttaa
    15301 ggaggcgagc gagccggcga tttctttccc gctggcgtga tgatcgatga tcgtcgtcac
    15361 tccgtcgcga agaaaatcgg cggcggccac aatgccgcta taatatgagg ctttgttatc
    15421 gatgtgacgg tctaacttcc accacatctg gtctaaaatt tcctgaaagt ttttgggatt
    15481 aaatggcagg gatagccccc gagcgaattt ggaatagatg tgggtgtggg cacagacgag
    15541 gcctggcatg agcaaagcac cacgggcatc gatgatttcg tatccttcat cgacgaagtt
    15601 ttccatcgct ccaacttgat taatgacatg atcgaaaata acaaaaccat tatctaaaaa
    15661 cgttgcaaaa tcatatattt tagcgttgat tatggctttt ttcattcgat atcccgtcct
    15721 ttaatccact gcccgcggcc tccctgatag acgcggttct caatgacaaa ttggccgcgg
    15781 accatcgtcg agatgacttg gccgtgcgag ggctgaccga tgtaaaggga gtaatcggcg
    15841 cggccgtgct gatcgagaat caaactgcgc ggtgcgggtt tgaagaagac gagatcggca
    15901 tcgtttccga ctttgatttc gcctttgcct tcaagcccct gaatgcggac gatattcaca
    15961 gtcatcttat tgataacggc atcgcctaag tggtggcgca taatcgtaaa ggagtgttcg
    16021 atgcccccga tgccaagcgg catctcaaag agcagtttgc cttttttgtc ggcgacattg
    16081 aaggcgcagt gatccgtacc gatcgtgtcg atatgtttct ggttgacgaa gaggagcttg
    16141 cgcgaaacaa agctgcgaag aggcggggca aatgtatata agtagccatc ttccctctgt
    16201 aaatcatcat tcgtaaacgt aaaatactgc gggcagcttt caacgaagaa gcgccgattg
    16261 agaatatcgc cataattttc aatgagagct tccaaagtat ggccgtcact taagtggacc
    16321 atatagagct tcccgccgtg gtcgcggacg agttgggcga gttttaaagc ctcacttctt
    16381 tcggcttctg acgaacggct gatcggcaaa tcctgatagg tgaagtcctt atcgaggcga
    16441 atcagttcat cattttcgat gtgcgccaag agcaagaatt tatatttttc ggatagttca
    16501 agcagcttaa tgatgtcttt ttcataagtc cggcgaccgg aatccgaata agtcgtaaac
    16561 aattttaaag tatgcatgtc gaggcttttc atcttcttga cgaattcctc aagatcaccg
    16621 ctcggatttt taatgcaggc atggaggcgg aaatcgacga cggagttttt tgctaaagcc
    16681 attcgcttat caaaggcttt ctgcagctca tcggcatttc cggccggatc gagaaaatcg
    16741 acgatggaag tgactccgcc ataggcggcg gccaccgttc cgtgatagaa gtcatcgacg
    16801 gatttatatt tgcctaagtc cagttcgaaa tggacatggg ggtcgatgat gccgggcagg
    16861 acttcgagtc catcggcatc ggtgacatta gccgcgtcca aaaagtcatc gctgatcaac
    16921 gcaattttcc cttctgaaat ataaaggtta tgcgtttccc agtgaccgtt gatgtagagt
    16981 ttgccattca tgattgcata atcgtacatg gttttactcc tctccttgaa cctgaacgag
    17041 ttcatagtca ttaatatcga agtggctggc gaccggcttg acgagatcgt cgcggacggt
    17101 gatgacgaga tcaaggccgc tggacagtaa cttcttcaag atgttgtgat atcctccgcc
    17161 gttgatttca agaacaccga cttcgtcgag ataaatcggc gagatgcgtt gagcgatgag
    17221 ttcatcgatg atgctttcaa gcatcgccag caagtccaaa ttgagataga atggtccgaa
    17281 tttgccggtt ttcgtgaagt ccctcagaaa gtatttttga tgaaccatcc acggaatgat
    17341 gttgtcgttg ctgaggtgaa gcatgttata accatagact tcattcccat tcattttttt
    17401 gatggcggca aagccatctc ccttgcggtc taagcgatag gtcttaagaa gcgtcgtggt
    17461 tttaccggca ttgacttttc cggtgataat tttaatcatt ccgagtcctt ctgccctgct
    17521 gtgtttggtg tttaagaatc aaaattaata tccttatagg agatttggaa tgacgactgc
    17581 caaaaccgca aaaacaaatg ttagcggcga caggacatac atcagccagt tgggggtttt
    17641 gagtggctct ttcctcatcg caagcaatgc ttgaatggag cggtaaatga tgtagataat
    17701 cgcaaagacg agcaattcat aggcgccatt aacaaagacg aagcgggcac ttgcttcaaa
    17761 gcttgagagc tgggcggcca ggtttccgct gatggcggat tgaacggctt ggttagccaa
    17821 gaaaagcgtg cgccaggaga cgattgctaa aacaattgct ccgagatgaa ttaatgtttg
    17881 attaaccggc gaggctttct tttcaaagag atagctgaca gcaaaaaccg tcaatgattc
    17941 caaaatgatc gaaatcatcg gcgtgtagac gcgcatcaag ccttcccagt gaatcggaaa
    18001 agcaaagttg atggccttga ttgccgcggc taataagccg acatagagaa tggcgcttct
    18061 gcggccggtg ttttgatagg cccagtacat cagagccgag ccgatcggaa acatgacgag
    18121 acccgaccac atgccaggca accagtgcaa aagatagccc atcgtggctt ctagtaagcc
    18181 ccagacggca ccgaaaaaga tgacggccat cactgtgttt ttaagcgttt ttttgttatt
    18241 cataacctga ctcctttatt ttttatttag cccctgaaat atcttttcgg gggtaacagg
    18301 taatttgttg atgcgcacgc cggtggcgtt agcgacggca ttggcgatgg cggcaggcgg
    18361 tgtgtcgatg ccgatttcgc caaccgattt cgcaccataa ggtccacttt cctcataact
    18421 ctcggcaaat tcagtcgtca gctttttaac gtcgagcatc gatggaattt tgtagtacag
    18481 gaaactgttg ctgtttagtt tcccacggcc gctgtatttg acatcttcga agcatgccat
    18541 accgagaccc tgaacgatgc cgccctcgac ttggccttta gccagcatcg ggttaatcgt
    18601 tacgccgcaa tcgacgacgc tgacataatg gagaatatcg tattcaccgg tttcggtgtc
    18661 gagttcaatc tcgacataac cagccatgaa aggaggtggc gatttgtgtc caacgaaact
    18721 ttcagtcgcg ctcagctgat gctgattttc gttgtaataa agtttattgg ccatatccgc
    18781 caaagtcaga cgcttatcct ccgtcctgaa ttccttgcca tcaaacatca cttcttcagg
    18841 cttggcattc agatattggg cagcttcttc aatgagcatt ttcttcatct ttaaggcagc
    18901 ttttaatgcg gcattgcccg agacataagt cgtgctcgaa gcataagcgc cgacatcaaa
    18961 cggagtcaga tccgtatctg aggaatagat gattacttta tcgactgtcg tcatcaatgc
    19021 ttcggcgacg atttgcgtga tgacggtgtc gctgccctgg ccgatttcgg tcgcgccgac
    19081 ggtcagatta taaaagccgt catcgttaag ctttatcgtc gcagcggcca tgtcgatata
    19141 aggaatgcca ctaccttgca tggcaatagc cattccgact gagcgcactt tgtgggggcc
    19201 gacttgaaca aacgggtatt tatctttcca gcctattaat tccgcgccgc ggtcgaggca
    19261 gtaaccgagt ttgcacgtat ccatgtccat ggctgtgcct tccgtgccct cgcccatgat
    19321 tgaaaaaatc gggctcgttt ctccttcgcc catcatattc ttgcgacgaa gttcgagggg
    19381 atcgatattt aattttttag ctaatatatc aatcgcggat tccaaggcaa aattaccttg
    19441 aatcgcccca tatccgcggt aggctcccgc cgagacgtgg tttgtgtaga ctacgcgtcc
    19501 gccaaagcga acggcatcga ctttattata taaaggcaat accttggaac cagtaatcat
    19561 gaagacggtc aaagcatgct cgccataggc gccggtatcg gacaggctat agcaatcaat
    19621 cgcttttagt gtcccgtctt tgttcgctcc caagcgcatt ctaatgcgca tcgggtggcg
    19681 agtataagag gattcaaata cttcctggcg ggaaaatata attttggccg gtttcttcgt
    19741 ttttaacgtg acaagcgaaa cgaaaaattc gccatgcaag gcttgcttgc cgccaaaacc
    19801 gccacccact cgcggtttga tgacgcggat tttgctgatt ggcagatcaa gcgtctgcga
    19861 gatgatgcgc cggacgtgat agggagtttg cgtcgccgtg tagatgacga ggcggttcaa
    19921 ataatccagg cgggcattcg cggtgtgagg ttcaagcatc gcatgggcct gtgcttgcgt
    19981 gtaaaatgtt tcgtcgatta cgacatcgca cttggcaagc gtcgcttcga catcgccaac
    20041 gtgcatgtga taggaggcgg cgacattttt cttcggttca aagccgatgg gaaacatctc
    20101 attaatgcca tcttcaggat gaataatgct tttgtgttca aaggcggttt caaaatcgag
    20161 gaccggttca agaatttcat attcgacttc aattaacttt aacgcctgat tggcgatgct
    20221 ttcgttaatg gccgcgacgc aggcgacttc atcaccgaca tagcgaacat actcatcaag
    20281 gataaactta tcgtgaggag aaggttcggg atagccttgg ccggctctcg taaacgactt
    20341 acgcggaaca tcgtgatgag tcaaaacaag ttcgacaccg gttaaggctt tagccttaga
    20401 cacatcgatg cttttgatgc gggcaaaggc atgggggctt cgtaaaatct tgacgattaa
    20461 ggaattgggt tcggcgagat catcggtaaa agagggacga cccatcataa ttcctttgcc
    20521 gtggactgaa ggcgttttgt gattgacgac tttcgttttc atttatgagc ctccaaataa
    20581 cgtttgatcg ctttaaactg ggcttgatag ccggtgcacc gacacagatt gccgacgaca
    20641 aaatgtttga tttcttcgtc ggtcggattt ttcagttcat ttcttagggc gaggatggtc
    20701 agagcgagac cggtattgca gaagccgcat tgatcggcgc cttcatcaga gaagaaatca
    20761 ctgatttccg agacttcatc ctggacggcc tcgactgtcg tcacgcttct tccctcgacg
    20821 cgcgcagata aaaacgagca cgataagatg ggacgcccat ctaataagac ggtgcaagcc
    20881 ccgcaggctg cttcatcaca agctttttta accgataaga tgtgattggc gcgaagggtg
    20941 tcggccaaat actcgccggg agcaatctca taagagatat ttcggccgtt aagaacaaaa
    21001 ctgactttca ttatttgctg acctcctcaa gaccgcgctt gatgtaagcc cggacgaggg
    21061 ctttgcgata ttcggcgcta gcggcgtttg tcgacgcata gagcaattct tcggaagcga
    21121 gagtggcggc ctgagcaatt tgctcatcgg acaacgcttt catgccatta actgtgtcca
    21181 tcgctttgac ggcgagggca gccacggatg gacggctgcc aatcgcaatg cgatattcat
    21241 tattcaaacg gctgatagca acattaatga cggcaaagtc aagagcggtt gttttaactt
    21301 tcttaaaaaa tgcgcggcct gtgctttttt tgataacaac atgggtgagg atatcgctcg
    21361 agcgtccttt ctgatttaaa aaatcagcga ggcctaattc cttttttgga taaaagatta
    21421 aagttgtgtc aagcgccagc aggggcgtaa tcaaatcgga aaaggggaat ttcccagcga
    21481 cggaaccgcc gacggtcgcg ccattgcgaa aggcgacgcc cataatctgg ctgacggccg
    21541 agacgaggat gcctccttgg atgttcttga cgagggggct catttcaaaa tcccgcaggc
    21601 tgaccattgc cccgacttcg atgtggtcgc cggtgttctt gatttgatcg aggccgagag
    21661 cgcttaaatc gattaaggca ttcgcggaag atgtcgattt ttttagccag agaccaccgc
    21721 ccagcaaaat gtttttggca tcttgaagca ggagctggtg agcttcttct aaggtcgccg
    21781 gtcgtaggta gcgatttgct tccatgtgtt gtttcctttc tctatttatc taaattttga
    21841 tagtcatgaa gggtatcgat gtccatcaaa atctgtaaat cctctgtctt gattatttca
    21901 taatcgtggc gattgcgaaa tactttgagg tttgaagtaa ccggctcatt aagcaattcg
    21961 tctcttagcg aatgcttaag aaaaagaggg tggcccgtct gtcctcgata ggcgggaacg
    22021 cgtatttctt tgcttccggc gagcatttta cgataggtat cggcagtgac gaacgggcaa
    22081 tcgccgggga gaacaaaaaa atcaccattt gtcttgcgga ccccggcttg gaccgagctg
    22141 aacatcccct gggcataatc gccgttatag acgatggtga cttttgcgta tggtttaaca
    22201 acctctttaa tggtttcgtg gaaatgcccg gtgacgacga tgatttcatc gacaaacggc
    22261 atcattcctt ccacggcatg ggcgagtaaa aacttgcctt tataaactaa cagcatttta
    22321 ttcgtctcgg cccggcttga gtagccggcc gctagaataa tcccttgggt catcaagttt
    22381 actccttgtt tgatatcggt tcttcccatt caacaatgtt gtctgagata accgcgtctt
    22441 actgtcgtgg tgtttacaac aactaaatta taaagggtgg ggcaattcaa tgcaaacgcg
    22501 tatttgcgca tgctgagaag tgcggatgat gagcgctcgc gtgcttgccc ctcaggcttg
    22561 agcgccccct gcctcacgaa cgcatgcgag aaaatacttt ccgtgcaata tattgcacaa
    22621 taaggcaaat cgttgttgat aattttgagg ggttgtataa tatttaagtt gtgcaaaatt
    22681 agcgcacgag aaggaaaaca accatgagca gatatgtcgt ctcactcggg gggaacgccc
    22741 tgggaaataa cgccgaagaa cagaagaaat tacttattaa tgtcgccgag ccgatcgtcg
    22801 ccttaatcaa agccggtcac gaggtgatca tcgtccacgg aaacggtcca caagttggca
    22861 tgatcaattt ggcttttgcc gaatcgaagt cgacacccga tatgcctttt ccggaatgtg
    22921 gagcgatgag ccagggttat atcggcttcc atctgcagaa tgccatttat aacgaacttg
    22981 ctaagcaagg cattgcaaag cacgtcgcca ccatcgtcac tcaagtcctc gtcgatgaac
    23041 acgatccctt attccagaat ccgacgaaac cagtcggcgc cttctatagt aaagaagaag
    23101 cggacatcat tgcccaggaa aaaggctatg tcatgaagga agacagcggc cgcggatatc
    23161 gccgcgtcgt cgcctcgccg attcccgttg atgtcgtcga aaaagagatg attctctcac
    23221 ttatcaagga tcagcatgtc gtcatatgcg ccggcggagg aggaattccc gttatctaca
    23281 aaggaagtca tcttgaaggc attgccgccg tcatcgataa agactatgcc agcgcgaaag
    23341 tcgccgcatt agtcgatgcc gattatctcg ttattctgac cgctgtcgat aacgcttata
    23401 tcaactaccg caagccgggt gaaaagcgcc tcgaactcat cacgatggca gagattgaga
    23461 aatattccga ggaaggctat tttgctaagg ggagcatgta tccaaaagtt caggcatgtt
    23521 taatgttttt gaaagccaag acaggcaaaa cggcgataat aagttctctt gaaaaagcca
    23581 aagaagcgtt cctggaaaag acgggaacaa tcatcaaatc gtaacttttt agcgtgaaaa
    23641 acaccgcaat tgctcgtttt ttcagtaatt tgcggtgttt ttttattcgt gcagcaagaa
    23701 ctatcttatc ttcgattctt tatgacaatt tgctttatgg gcatcaaata taatcggaac
    23761 aagaatcaca aaattatgct ttttacgaat gaatctgaaa ccgcaatagt aagcaagata
    23821 tcgccgtatt taagtgacta aatttctgaa tcatataata taattaacgt cgataacaaa
    23881 aaaagaaaaa gagccatttt atggctcttt gttcgctacc gtgttagtta atgaatgacg
    23941 cggaggctga tgataccggg cagttcgccc agttgatcac ctaagcacac accttctgag
    24001 cattcgcctt tcgtcttgac aaagtcgacg agcgtgacgc caaaggcgcc tttgactttg
    24061 ctggcgattt gtttgacgac gccgcgggtt tcgacaatct tgatgatctg gcccgaaagc
    24121 ccttcgttag cgtcgtggag caaaacgatg cggtgagctt ccgttttcgg accaagctcg
    24181 atatttggat aattcacgga attgacgatg ttgccgtttt cgataaaatc gacaatttgt
    24241 ttgacagcca ttgttgcaca gttgtcttcg gcttcttcag tcgaggcccc gagatgaggg
    24301 atggcgacaa cgccttccat attggcggtc gcatgattgg gaaaatcagt cacatagcga
    24361 cggactttgc cttcttttaa agcttggcga atgtcatcat cattaacgat ggcatcgcgc
    24421 gctaagttga tgatgacaac accgtctttc atcttggcga tactctcgcg gtttatcatg
    24481 cctttggtgg caggaagaag tgggacattt aagctgatgt aatcggctgc agtgtatagt
    24541 tcatcggcac ttgcgaccaa gcggagaccg gcaggaaaca gatcgcggtt tttatcaatc
    24601 gttgcttgcg acggctcata ggccgcaaca ctcataccca tgccataggc accttgagcg
    24661 accagagttc cgaccgcgcc taagccaaag atgccaatcg tcttaccaag aagttcggtc
    24721 ccggcaaaag cggccttggc tttttccatg tttttgttaa tctcgacatc atctttattt
    24781 gcttcaaccc atttcatgcc gccatgaata tcgcgggcgg caagaagcat tccggcaatc
    24841 gttaattcct tgacggcgtt ggcgttcgcg ccgggcgtgt tgaagacgac gacgccggct
    24901 ttcgcgtatg catcgagcgg aatgttattg actccggctc cggcccgggc gacagcgacg
    24961 acattatcag gtaaaatcat ctcgtgcatg acggctgagc gcaccaggat ggcatcggcc
    25021 tcatttattt cagaggtgac ctcataatta tgaggaagag tctttaatcc gacgggggat
    25081 atcttgttta agcaaagtac ttttttcatg gcgcgaccta tttgacgtgg gcggcttcaa
    25141 actcgcgcat gaatttaact aaggcttcga cgccagcgag gggcatggca ttataaatcg
    25201 aggctctcat accgccgacg gagcggtgac ctttgaggtt ggcaaaaccg gctttcttcg
    25261 cgccttcaag gaaggcttca ttgagttctt cgctgtccgt cctgaaacag acgttcatta
    25321 atgaacgcga acccttatcg accgtgcctt taaaaatctt gctgttgtcg aggaagtcat
    25381 agattaaagc ggctttcttc tcgtttcttt ctttcatggc ttcgagtccg ccgtgagcga
    25441 gaatccattt aaagacgaga ccgcagatgt agataccgta agtcggaggc gtgttataca
    25501 tcgagccagc atcggccagc accttatact tcaataaagt aggcgttccg ggaaggggtt
    25561 cgttttccag caaatcttcg cggatgatga cgatgacgac accggccgga ccgacgtttt
    25621 tctgggcgcc ggcataaatg aggccgaact tcgtcacgtc catcggttcg ctcagaaacg
    25681 aagaggaagc atcgttgact aaaatcttac caggaaattc gggatacttt ttgtatttgg
    25741 tgccaaagat ggtgttgtta tcgcacatgc agacaaagtc tgaatcgggt gaggctttga
    25801 cattatcaag attgggaata taagagaaat tcttatcttc agaggaggcg atgacattga
    25861 ctttaccaaa gatctccgct tcttttttgg cggcttttga ccagtgaccc gtcaaaataa
    25921 agtcataaat tctgttcttt gaaagattca tcggaaccat cgcaaactga acgttgcctc
    25981 cgccttgaac gaaaaggact ttgtagttgc cgggaatgtt taaaaccttg cgcaaatcgg
    26041 cttcggcttc gtcgatgatg gcctgataag ttttggagcg gtgcgacatt tccataaccg
    26101 acattcccga tcctttataa tcgagcatct catcggcggc ctgttgcaac acctcctcgg
    26161 gcaaaacggc tggcccggca gaaaagttgt agatacgttt catatgtttc tccttccgtt
    26221 atctatttta ttctcttgca ttcgagatag taacgcttct caacggcatg ccccatcgaa
    26281 aaggttttgc gaggcagcac atcgcctttg gcgatataat caaacaaatc ggctttagta
    26341 agggccggca tcgccaccgc gaccgaggtc ggatgtttgt cggcgacttc atgggtgtaa
    26401 tcgatatcgt gaatgtaatc gacggatgaa ccgggagtat ttttaacata ggtatcgatg
    26461 taatcttgaa tgaggcggta ggcgaggggg gcattcgccg gtaccgatag cggcgattgt
    26521 cctttcggct tgcagtagac atgtgtttca tgagtgccat ccaccacttt tctaaggccg
    26581 ggaacaaagt cggatttgac ttcaaacacg acgcggtgaa taggctcaaa atccaagcct
    26641 tcatcataga ggttaatagc ttcgaccaag gcaaagcggg ccggatgtgt ctctctttcc
    26701 ttggcggaca aaccgatttt taagcggtcc caatgggctt tcgcggtcgc taaagagtga
    26761 tttccgtcac cgacgacaaa catcaaatcc ttgccgtttt cttcggttaa ttttgtgaaa
    26821 gcgtcgatga ctttttgtgt atctttaact tgatatccgc gcaggtggcc cccgttctga
    26881 ttaaggtcaa aatcgtacaa taacggcatt tctgcacgct tttcgtagat tttttcaata
    26941 attgacttct tgggatcatc aaacaagaag agcgtatgag tgagttcgac cggagcattt
    27001 tcgcgaatct tcaaccgcgg cggaatgcgt tcgacaatcg tcgcctcggt cgcccgaatc
    27061 aaagcttttg ttcccttttc gtaggagtaa gcttctaaat ccacggagat gactaaaccg
    27121 agtcttcgct ttgtgtgcgg ggtgttccgt tcgacgagga caaagcactc gccgatgtct
    27181 ttaagaacgt cttcttttaa gtagcggtcg atgtttcgat tgatgccggt aatataatct
    27241 ttttcattta ccaaagtgag ataagcctcg ggaaaaatca tgtgaaaagt cgatggcttg
    27301 tcaccgattt tttctttaag accgtcccag taatcgcatt gactcgtaaa ctggtcgcag
    27361 gcgataacgg cgaaggcatt catgtcgacg ccgtctttgg gaagcaggat atgcggaggt
    27421 cgaatcggat tgacgatttt cattattaaa tgacaccctc ttgaagcatc gcgtcataga
    27481 ctttgataaa gccggcgatg ttcgcgccga gggccaggtt tttcggctgc ccgtattttt
    27541 gagctgtctc gtggcatttg cggaagatat ttttcatgat ttgttcgagt ttttggtcga
    27601 cttcttcata agtccaagac aagtgcatgg cgttctgact catctccagg ccggagacgg
    27661 cgacgccgcc ggcattgctg gctttgccgg gagcgaagag aataccattt tcgttgcaga
    27721 agcgaatggc ttccaccgcc gtcggcatgt tggcgccttc gacgagcata tagcagccgt
    27781 tagccttcag ctttttggcg tcttcgaggt caatttcgtt ctgagtcgca catggaagag
    27841 cgatatcgca gggcatgccc cacattagtt tcggggtcgg aacaaacttc gcttcaggat
    27901 agatcttcat atattcgaga gaatactcgc ctttcggctc agacaactta tcaattaatg
    27961 gcacatcgag accgttttcg tcatagacga agccattaat atctgacatg gcgacgactt
    28021 tcgcccctaa ggcgtgggct ttgacggcgg cgtgcttgcc gactttgccg gaaccggaaa
    28081 tgatgacgcg cttacctttt aaggtatcgt catggaatgt ctttaacatt tctgaaacga
    28141 aataaagaag gccgtagccg gtcgcttctt tgcggcccag ggaaccgcca taggaaatac
    28201 ccttgccggt aaaagtgcca acaaagtcgt tagatagttt tttatacatg ccaaacatat
    28261 agccgatttc gcgggcgccg acgccgaaat caccggccgg cacgtcggtg tcgggaccga
    28321 tgtggcgaaa caattcggaa acataggctt ggcagaatct cataatctct tcatcggaac
    28381 ggccgcgagg agtgaaatcc gcaccgccct tgccgccacc gagaggaagg ccagtcaagc
    28441 tgtttttgaa ggtttgctca aaggctaaaa acttcagaat cgagaggttg acggaactat
    28501 cgaaacgaat gccgcccttg taaggtccga tcgccgagtt aaactggacg cgccagccct
    28561 tgttgacttg gactttgcct ttgtcatcaa cccaggcgac gcgaaaggag atgactctct
    28621 ccggaattaa gatgcgctca agaatggcat acttctcaat gtttggttcg tcatcgacca
    28681 tgtagtccat cgaatcaaaa aattcctcga ccgccgagat aaactcaggt tctgaggaat
    28741 atttcttttt gacatcttca aatagtttgt tgagataact gctctgatac acttgtggtt
    28801 tcctcctgac gtttgtcgcg ggatggcttt cgcgccttcc gagacatacg atgaatagtg
    28861 ttaagcgatt cggtaattat aaattacctc ataatatatt atactgaaag cgcttactta
    28921 tcaaggtcaa atgaatgacg ctccgattct ttgaatcgcc tgtcgttttc tgtttgaaga
    28981 ctccaaagaa gaaacgcaac tgcagatggg ataatggagg ccaactgcag gctctcggcg
    29041 tctgagcacg caaataaaac cacctcgatt ctatatattt tgttaactaa ttttcgtata
    29101 tcaaaaccta agatgaaata gactggatta
//
LOCUS       DAOSSL010000007        28475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1830_length_28475_cov_15.719599, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000007 DAOSSL010000000
VERSION     DAOSSL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 28475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28475
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_28475_cov_15.719599"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="PLH17_01530"
     CDS             <1..2088
                     /locus_tag="PLH17_01530"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOR20496.1"
                     /translation="MAKKYHTGQVRKSGEDYIHHLIEVGYILAKLQAGPATIAAGFLH
                     DVIEDTPAELEEVQSKFGEDVSKIVESLTKIQRMKLSHREYEDFEAEDHRKIFLGMAK
                     DVRVIIIKLADRLHNMRTLSALENKRQLALARETLEVFVPIAHRLGINTIKSELEDLS
                     LKYLEPDKYNLISDLLDKKTRNRTKSLDSLKKRVADILFEAKIPFQMESRLKTIYSIY
                     KKIYLKGYNFDDIFDVLAIRVVTETELNCYEILGIIHATFKPIPGRFKDYIAVPKPNM
                     YQSLHTSIVSGDGNIFEIQIRTKRMDEIAETGIAAHWRYKEGTNYNAKEEQRQIEEKL
                     HWFRDFVTISNEQEGDAKEYMDTLSKDIFEANVYVFTPKGKVIDLPNGSTPIDFAYRV
                     HTKVGDAAVGAIVNGSLVSLGTVLKTGDVVEIRTSNTSSGPNEGWLKIVRTAQARSHI
                     KKFLLKKNAELMKDERIAKGKQSAIDAFRDRGVEEAEMMTMLNNPNLLNNFHVKDLDE
                     LFVTMYSKNPTPSAVIDFLNIRRKPTIFKLDKAPTPKDDDHVPVFVSGAGKIAINLGS
                     CCTPIPGDDIVGYITKGKGVTVHRVTCPNIANERARLVDVFWRESEHLDTYPVDIKIE
                     SADRPNLLIDIMSMLSANKISVSTITAKLHPQTMNTTISATIFVSDAKRLTDIFNILL
                     NINSVYEITRVIH"
     gene            2232..3533
                     /gene="hisS"
                     /locus_tag="PLH17_01535"
     CDS             2232..3533
                     /gene="hisS"
                     /locus_tag="PLH17_01535"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOR20497.1"
                     /translation="MEVKPVKGTHDIIGNDIILYQRIEDALAKIAHTYGYLEFRTPII
                     EHTELFARSVGDSSDIVRKEMYTFLDKGNRSLTLRPEVTASIIRTIVNNKLYSSQDFP
                     IKASYLGPTFRYERPQAGRYRQFSQFGVEAVGLDSHLIDVEVIALGYNALKNLNFKDI
                     TLKINTLGDDASRNQYREALRGYFAGHIHEMCIDCQERLNSNPLRILDCKVEEDRKII
                     KNAPRIQDFLSEQSKERFSKTINLLDSLNIKFDLDETLVRGLDYYSEVVFEYHYIAPD
                     GKTIGALGAGGHYSHLVEELGGPNLAGVGLSFGVERLAQVVASENLLFDAFPFLAFYI
                     MPIGEVNIEKVYELNEKIHRLGYPSEMAYESKALKALFKKAEKRSALFAILIGEEELE
                     KEMLTIKNLRTKEQASVALADFDSVANQMVEEELIELNEER"
     gene            3537..5264
                     /gene="aspS"
                     /locus_tag="PLH17_01540"
     CDS             3537..5264
                     /gene="aspS"
                     /locus_tag="PLH17_01540"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOR20498.1"
                     /translation="MERTCYNASLSINDVGREVTLLGWVSKRRNLGSLLFIDLRDRSG
                     IIQVTVKDDVKVPDVRNEYVIEVKGRVAKKDVANPKLKTGEIEIIASAITIINEAATT
                     PLIIDDDTDALEDVRLKYRYLDLRRPLMQEKLKVRAKIVRAAHEFLDDNDFIEVETPI
                     LTLSTPEGARDYVVPSRIHHGCFYALPQSPQLFKQLLMIGGLERYYQIARCFRDEDLR
                     ADRQPDFTQIDIETSFLDETQILTLMEGLLKKIFKDVINYDLRLPLRRLSYDEALNRF
                     GSDKPDTRFGLELKDVKDLVSKTPFPLFNSSAHSKAIVIPNLAETTSRKNIDELQLEA
                     KKFRLSSFIVLKVKDEHLEGSAAKFFAEEDGRALIKSLSAKHNDLIIIACSNSLANVC
                     FGLGALRSRYARELGLIKPNTYDLLWVVDFPLFEKDQDSGKLSPLHHPFTRPTAATAE
                     WLSSDPARVHASAYDIVINGYEAGGGSLRIYDQKMQKTIFEVLGFSQEDIKNKFGFFI
                     DAFKYGTPPHGGIAFGLDRLTMILSGTDNIRDVIAFPKNLRASCPMTDAPSKIDQAQL
                     DELGIKFVE"
     gene            5324..7309
                     /gene="tkt"
                     /locus_tag="PLH17_01545"
     CDS             5324..7309
                     /gene="tkt"
                     /locus_tag="PLH17_01545"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOR20499.1"
                     /translation="MKTIDKTNLDQLAVSAIRAMCIDITNKAKSGHPGMPLGSAPILY
                     TLYKNHLIANPHDPNWINRDRFVLSAGHASALLYAMLHVTGFDLSIDELKAFRQIDSL
                     TPGHPEYRHTKGVEATTGPLGQGIAQAVGIALAEQAVQAQHVEGHKLINHYTYCLCGD
                     GCLQEGISQEAISFAGHQRLNKLILIYDANKTTLDAPLEASFSENVELRFLASNWNVL
                     KVKDGNDLDALDRAISKAKKSSDYPTLIIVNTIIGYGSSKQGTAKVHGNPLGVEDGQK
                     AKAFYGYDYPDFTIPEEVYQVFRDTFEKRGIEEYQKWKNVRDTYKDKHPEEYRVFEHA
                     FKGDVTNYIYNSIPKVEAGSSDSTRGISGHILNELHNTIPFIAGGSADVASSVMTKIN
                     KGCDFTPACREGRNINFGIREFAMAAIQNGMLLHGGLKTYVGSFLVFSDYLKPAVRLA
                     ALSRLPAIYLFSHDSIALGEDGPTHQPIEQLAMLRSIPNLRVIRPCDNAETYGAWRIA
                     LKSKETPTAIILSRQGLPEMEGSTMEAVAKGGYIISPEKEKLMATIIATGSEVALAVK
                     AQKLLLDRKIDVRVVSMPSIGLFEAQDAEYKKNTIGVTKDKVVAVEMLTSFGWYRYAD
                     TVMGIDTFGASAPMADVVKKFDFTSERLVSLVEKIAK"
     gene            7367..7702
                     /locus_tag="PLH17_01550"
     CDS             7367..7702
                     /locus_tag="PLH17_01550"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOR20500.1"
                     /translation="MADYVYIENYSKYGVMGISRRVFETIAQTATNRVKGATVSANKS
                     RLFTLHRPIQAILRSNGLVDIRIDVAIKSGVNVSEVCLKIQEEVANSLMAMTEMVPFN
                     INIKVASIQ"
     gene            7728..8258
                     /locus_tag="PLH17_01555"
     CDS             7728..8258
                     /locus_tag="PLH17_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20501.1"
                     /translation="MKSNKPQPTYAEAHNKAVRLYRKTAIFLMWAGIISILATVIALF
                     KSEAGYGMSLAINIFLNRLIHALGLSNLATSLFIFALSVVTGALFAVLGFFAQQGKKI
                     FLFIGAALYLADFAAVFLVYGANWGTTYAFTIATHLVILVALAIAIYEYFNVIAIEKR
                     FHGAATPNLDEANGTE"
     gene            8260..8670
                     /locus_tag="PLH17_01560"
     CDS             8260..8670
                     /locus_tag="PLH17_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOR20502.1"
                     /translation="MSTNNTRNQNQILAMSCIYDYLTRVEMGEDIDVKALLERVCDAP
                     FADIDTFVSEVFIKVVRNHEEIVAMLQANMNKWKFSRLNRIAQSILLLSVAHYRYVGE
                     VDRSAVIDIAVRMAKRYLDNADYKFINAVLDSTL"
     gene            8667..9881
                     /gene="xseA"
                     /locus_tag="PLH17_01565"
     CDS             8667..9881
                     /gene="xseA"
                     /locus_tag="PLH17_01565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOR20503.1"
                     /translation="MKSGEFYTVTSLNRHIKEILDGEVSLRLVKLKGEVSNLKKYPSG
                     HFYFTLKDENSVISAVMFYNDAIRIGFNMKDGDEVLAYGSVSVYPQRGSYQIYVSEIE
                     LFGQGALLVELEKLKQKLKAEGLFDENRKRPIVSYPESIGIISAPGSAAIEDMIKNIH
                     RRYPIARIYFFPSLVQGSTAPKDLLRAFELTKSYDLSTLIIGRGGGASEDLNAFNDEV
                     LVRALATSRAPIISAIGHEIDFTLVDYVADRRVSTPTGAAELATPDKREIYETLNENL
                     ERMSLALQNKVHTLKERLNLLAKRAFFIDPKSIYENKVRDVREYRVRLVNAMQNSYLI
                     ASHEVRSSKQRLEALSPFGVLKRGYSIMTDESGKVIDSVNKVETGQNVLTNVKDGIIM
                     SRVTAKESKKNG"
     gene            9874..10071
                     /gene="xseB"
                     /locus_tag="PLH17_01570"
     CDS             9874..10071
                     /gene="xseB"
                     /locus_tag="PLH17_01570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOR20504.1"
                     /translation="MAKENEINFEKMLARLEEIVATIESKTLPLDESIALYQEGKSII
                     KTLEEALKDAEGKIADILKVE"
     gene            10114..11994
                     /gene="dxs"
                     /locus_tag="PLH17_01575"
     CDS             10114..11994
                     /gene="dxs"
                     /locus_tag="PLH17_01575"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOR20505.1"
                     /translation="MAKPSSKRIIDIDIRDIKGPEFLKELNYRSLDLLSEKIRREIID
                     KTSKYGGHLSSNLGVVEITIALHRMFNFKHDKLIFDVGHQSYTHKILTGRSLEYLRNR
                     NGVSGFQKICESYYDCFEAGHSSTSLSAAQGFALNRDARGEKYDVVAVIGDGSIVNGL
                     AFEALNNISHNNNKVIIILNDNEMSISRPTGGMGRFFAKISTASGYTNFKISYRRFMF
                     KTRMGHTLYNFISRIKNAIKQKLVPTTYFDDMGFQYIGCIDGHNIKALEKAIARAKRT
                     PKSVVLHVATVKGKGYRYAENDTTGYWHGVTPFEIESGKPKNLHPEELSWSHLFSDLL
                     TALMAERNDLVIISPATVKGSGLEDLFSTYPKRTFDVGIAEGHALTLASGLAVAGTHP
                     IVSIYSTFMQRAFDQIIHDIARMNLNITFLVDRSGLVGLDGETHQGIYDESFLLSTPN
                     TVVTMPSSDKEAKYLLNESLKQHGPFFIRYPRDLLKKNHLIQEENHQFGKWIMECVAN
                     SKRVAVIAVGQLLRSLKGLIIEADLDVTLVNALFLKPHDQEMLSSLSEYETIVIYDAY
                     ATEFGFASYLKTKLCEMSYKGQIKSFAIPDVFVTQAKISEQLEELGLLPKQVFEEIKK
                     LL"
     gene            12060..13262
                     /gene="dinB"
                     /locus_tag="PLH17_01580"
     CDS             12060..13262
                     /gene="dinB"
                     /locus_tag="PLH17_01580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOR20506.1"
                     /translation="MSKIIVHIDLNAFFATAEEIRDPSLVDKPVIIGGDGRKGIVSTA
                     SYAARKFGIRSGMPTFQAKRLCPHVLILPVDFRYYGLLSREFFAFVRKYSKKVEKASV
                     DECYIDMTEALENVEDVRAYFLNMQNKLLEKTGLKCSIGVGPTKFLAKMASDMKKPMG
                     LTILRRRDIPRLLYPLSIRDFYGIGRKTSPKLEAIGIKTIGDLARRINSDDPEMHLLL
                     GKFFFVIKDWVNGYGNDEVDLEPWDPKSIGNSSTFMHDTDDFMEIKEMISTLAKEVSE
                     RAQNERKQGSTVQLVIKDTEFKTINRSLTFARPTNEYEVIFDKALTLLERHYKGQPIR
                     LVGVTLQNLIDPRDLVIQMSLFDYRKHEEESETKLLINELNRKLKKPMLIRASEVEHD
                     KRGNKDED"
     gene            13252..14133
                     /gene="xerD"
                     /locus_tag="PLH17_01585"
     CDS             13252..14133
                     /gene="xerD"
                     /locus_tag="PLH17_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOR20507.1"
                     /translation="MKIDDAIDLFFQHLTVEKGVQPQTIQAYANDLKQFFKVFKDKES
                     TDDLLVSDLTDFIKIQSKQNRANATILRRLSSTKHFYRFLEKEKLLPDAVPKIESPRA
                     IKKLPLSLSVEQVESLLEAPDLEKPEGMRDRAMLEVMYSSGLRVSELINLERSQVNFT
                     HGIVTIYGKGNKERRVPIGEYALEYLVHYIEEARSKNIGKNTKYLFLNRYGKELSRQY
                     FFRQVKKYATYAGIKEEISPHTLRHCFATHMLDKGAELRAVQEMLGHQNIATTQIYTH
                     VSSNRILSAYDLYAKRK"
     gene            14166..15359
                     /locus_tag="PLH17_01590"
     CDS             14166..15359
                     /locus_tag="PLH17_01590"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOR20508.1"
                     /translation="MNKYKRMFVIVIDSVGIGEMPDAEKFGDKGANTLVHTAREAGGL
                     KVPVMNSLGLGDLAPILGTSIVKHPHSYALRLRETSAGKDTMTGHWEMMGINTTKPFQ
                     TFTDTGFPQSLIDELAEKTGHKIIGNKAASGTEILVELGEQQMRENSLIVYTSADSVL
                     QIAAHEEVTGVKELYRCCDIAREICMKPEYFVGRIIARPFIGTNKHNFKRTANRHDLA
                     VSPTGTTTLDVLKNNGFMVSSIGKINDIFNTMGVVKAQKTVSNLDGMNKTIEEAKNHD
                     FTGLCYVNLVEFDSEFGHRRNAIGYAKALEEFDVKLGELLKVLKDDDLVMVTADHGND
                     PVHHGTDHTREKVPLLIYSKSIKNGRYLDERETFAVIGASVLENFGLKKTPNQIGAPI
                     MEIFE"
     gene            15360..16313
                     /locus_tag="PLH17_01595"
     CDS             15360..16313
                     /locus_tag="PLH17_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20509.1"
                     /translation="MKTKSIILPLAAITLSACGGPAPEITIRTISPAGAPTLAFYDQG
                     SNENFVTNSTPSNVLAELQTNYYNAVVFDSINALKSIKNNDLDFKLAKLITGGNFYLA
                     SINKNEGEMPTADDVVVSFGENLIPDLVYRKLSEYWEIENTPYYVPSVSEALGVLKTG
                     VYASNPVDFVFIAQPALNIALNDATAATYGNVKVVKNIRSEWQEYTGQSAIPQAGLFV
                     RASHYELNPTVFENYVDEMVARIDVAIDTPATAKAALDAYGDAQAQAAKFGFNSNIML
                     TTQSDGANGFGLVRGTEEVDVNEFLETLGLETFADSYFLGL"
     gene            16329..17105
                     /locus_tag="PLH17_01600"
     CDS             16329..17105
                     /locus_tag="PLH17_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR20510.1"
                     /translation="MNKTKAFFQKHGAVIYTVLGIGFVFLLWWLTSLIVKATVLPTFP
                     GPEDAIPLFFTLFGEATTWLAVGGTLLRLVISFAIAFLLGSLMGVIAGVHKSFAHFMR
                     PLVITLRTLPTAAVILILIVLLRPLFAPIIIVFLIIFPISYESMATGIKNVNREVREA
                     AKIDGATRLQSIIRIKIPLSMPYVLLGIVSSLGLGMKISIMSEIIAGSNRISGLGRLI
                     HLAYIEADMTRIVAISIFAIILIGLIDLGMYFAKKKLKGR"
     gene            complement(17675..17998)
                     /gene="rsfS"
                     /locus_tag="PLH17_01605"
     CDS             complement(17675..17998)
                     /gene="rsfS"
                     /locus_tag="PLH17_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOR20511.1"
                     /translation="MNSDKYTKGILQLLEETKAEDIQIIDVRDKTPFSEYYILVTATN
                     PRQMNALKEAVVEKIETLKGKINHVEGNENSGWILVDAYHVIINIFSKEERERISLEQ
                     ILSRR"
     gene            complement(17985..19103)
                     /gene="nadD"
                     /locus_tag="PLH17_01610"
     CDS             complement(17985..19103)
                     /gene="nadD"
                     /locus_tag="PLH17_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOR20512.1"
                     /translation="MESVILFGGAFDPVHNGHLRMAGAASFLLNADVIFIPSKSPRWK
                     NPSVGIDHRLKMLALAIRQNGLSGTTISDYEIRSEVEVNYTIDTVRYFVNKYPKKQFY
                     LLIGADQVNQFHQWREPEEIVKLLTVVYIPRPDIKINEGNVTKYKIMSLPYNHTGEVS
                     STKIRNLQSVDTPSAVLDYIEKNRLYYIEKIAGMLDKERFEHSLSVAHLARSIAYKNE
                     RNDYPKAYIAGLLHDLGKGYDTAQTIAIMKRQYKDYVNQPEFSYHQFVGSYLAEKEFG
                     IKDEEVLDAIRFHATGKPHMSPLGKIIYCADKIDPLRGFNSRPLIKKCYQNYYLGFVV
                     VLSANREYLLANGKDINNPLTDACMQLYLGKGKDIEFR"
     gene            complement(19103..19384)
                     /locus_tag="PLH17_01615"
     CDS             complement(19103..19384)
                     /locus_tag="PLH17_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOR20513.1"
                     /translation="MLSNKDRQELFALSNRLKTRYIIGKDNLDEKVLEMLDKALLANE
                     LIKVSVNKNASQEADYFATAICAKLDCELIKIIGKTIVLYRQKPQRNKK"
     gene            complement(19374..20513)
                     /locus_tag="PLH17_01620"
     CDS             complement(19374..20513)
                     /locus_tag="PLH17_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOR20514.1"
                     /translation="MAKLNIIRRCYSCGVVLQSKDPQKDGYVENEFLQDLSKGVLFCS
                     KCFHSEKYNLAPREASLDHEFFTLIADAQATDALIVYVINLFSFEAGFIRALNHWLSG
                     LDILVLANKRDILPDDIDDEQLKEYVAHRLRVEKLKVLDVKLVSASANYNIRDVIDVI
                     KDLRRRRDVYIIGQKHSGKTTLMEAFLKEYKNLSRTNIITQNYPGTNLKVMQIPIDQS
                     TYFYDTPGLGNDNSVLEKVEKPVLKAIVPVKKIEKRRFTITKTQSLFIGGLARIELVE
                     GEKTAVGCYFSDDVDIKKIVLPDANKQFLRTLSKDLLHPTSKNLQSLKDFDIYDIVVD
                     EEGSRDIGINGLGWFSFIGANQTFRLYLPKGVSFYTTRSKIQDVK"
     gene            complement(20513..21034)
                     /locus_tag="PLH17_01625"
     CDS             complement(20513..21034)
                     /locus_tag="PLH17_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOR20515.1"
                     /translation="MNKRFIPFCVAKSIYDIEPAFFQKHNISILFVDLDNTLDAYHQS
                     HPTDKAFALKEKLSKAAIEMIVVSNNTSKRVSTYAKELGVRYAGSLYKPFAFKLRKFI
                     KKNGIDISKVMMIGDQIITDVACGNGAGIRVILTERLVEDDQWTTRFNRWFDAPRYQK
                     MKKQNLLISWEER"
     gene            complement(21153..23750)
                     /gene="parC"
                     /locus_tag="PLH17_01630"
     CDS             complement(21153..23750)
                     /gene="parC"
                     /locus_tag="PLH17_01630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOR20516.1"
                     /translation="MVKKAPDTVEVIKEKISNQPLEEVMGDRYATYAKYVIQDRAIPD
                     VRDGLKPVQRRIIYAMYESGNISTRPTKKCAHTVGAVMGKYHPHGDASIYDALARMSQ
                     DWKVRVPLVDFQGNNGSIDGDVPAAYRYTEARLSEIAEELTRDLEKKTVDMDLTFDDT
                     DFEPSVLPARFPNLLVNGSEGIAVALATEIPPHNLREVVEAVIWRLTHVNVTPLDLMR
                     FIKGPDFPTGGLIYEGEGLQNMYETGRGRIEVAAKTQIINEKGLKQIVITEIPYKTVK
                     ISLVYELDKIRHGKEIDGIAEVRDESDRKGLRIVIDLKKDAKPEVILAYIFNKTQLKS
                     SYSANIVAIVNGRPKTLNLLQIVDAYIDHQVEVITRRSHFDLDKSQARLHIVDGLIKA
                     ISILDKIVEVIRASKDKTSAKENLQKKFDFSEAQSEAIVMLQLYKLSNTDISTLVKEK
                     ESLEKTILKLQGILEDRKKLDRVIIADLRAIAAKYGDDRRTQIVEKDINLQIDKRDLI
                     AKEDVMIAITRDGYIKRSSVKSYRGSGVDSLPGMKEDDILVYRGQAVTTDFVLAFTNR
                     GNYLYIPIHEIIDTKWKEEGKHINYLISLEYKEKIVCAFAVNEFRGDLFLALVSKKGQ
                     IKRVRLSDFVVIRYARPITAMKLIGDDEIADVVVTSGDSNLLVLTSTGGAILFNENDV
                     SIATLKSGGVKAIAELKPAVIAKILVFEKDEKAKIALLTHAGHIRIYDYANTPLTVRL
                     GKTTPIYPCFKNEPHYLIFAQKISAKDEKIVWRVQANNKAIIEVPVEEFYVTPRNKYA
                     KGTITLPRRSHLAIVFRQDDIMIDQTIIAHEPPIKEKPIAVSNGVELDENGEEYKQIS
                     IFEEDETKK"
     gene            complement(23750..25693)
                     /locus_tag="PLH17_01635"
     CDS             complement(23750..25693)
                     /locus_tag="PLH17_01635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOR20517.1"
                     /translation="MTEKTIKNTYTAADIDILEGLSGVRKRPAMYIGSTNSSGLHHLV
                     WEVIDNGVDEALSGFGSKITVIIHKDGSLSVEDEGRGIPVDLHTQSNMPAVQLIFSTL
                     HSGGKFSDAVYKSSAGLHGVGASVTNALSEFLDVTVYRDSSIYHLRFENGGKLVTPLE
                     IIGKTNKHGTLVRFKPDATIFTSIDFKWDTIYNHLQESAFLLKKVRFILKDERTDLSH
                     EFYYVDGLKEYVATLNTNKKPLGNIVSFEDEANGIIIECAMQYCIEDYNESIYSYANN
                     VRTRDGGTHETGFRIGLTRAVNDFVTEHSLLKGKNKIEGGDIREGLTAILSLKIPETL
                     LQYEGQTKSKLGTPEAIQAVNNFIYSKLTYYLNENKEFAVNLVKKISDSRDARLAARK
                     AKDEARSTKKARNDLIISDKLTPCQSKEYALNELFIVEGESAGGTARKGRDRLHQAIL
                     PLRGKPLNTDNVTMERMLKNEEFSTIISTIGAGVGQNFDIDESKYGKIIIMTDADTDG
                     AHIQTLLLTFFYHYMRPLVNKGKIFVAVPPLYRVYKEVNKTIVYEYAWDDRGLEKAKK
                     NIGAGYKISRYKGLGEMDADQLWETTMNPRTRQLVRVKIEDPLLVERRVSVLMGKDAA
                     VRRKWVEENVDFNKVDHFIEEIK"
     gene            25797..26510
                     /gene="plsY"
                     /locus_tag="PLH17_01640"
     CDS             25797..26510
                     /gene="plsY"
                     /locus_tag="PLH17_01640"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOR20518.1"
                     /translation="MDIIYNIAIGLGSLIIGYLIGSFPTAIVIGKVFFKQDPRDFGSK
                     NAGGTNAGRLWGKKAGLAVMIVDITKAVLSIWAAYLLINLTGLANAVALWRGGMAYYY
                     LASLGATLGHCWPLYANFRGGKAVSALAGMVVATSWFLSIFGMVFFVILKNKKFVSLS
                     SIIASSTVVLAALAMFLTDYFVPGSFNFGMWGNGKLFLVGWDYFATILIITIILIFRH
                     HENIKRLARNEENKVRWIK"
     gene            complement(26760..26975)
                     /locus_tag="PLH17_01645"
     CDS             complement(26760..26975)
                     /locus_tag="PLH17_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOR20519.1"
                     /translation="MEVWSWILLIVLALIGGLVAGFFGSRWLFKRELAKNPPINENMI
                     RAMFLSMGRKPSEAQIRAVMNNMKNSQ"
     gene            complement(26988..27545)
                     /gene="efp"
                     /locus_tag="PLH17_01650"
     CDS             complement(26988..27545)
                     /gene="efp"
                     /locus_tag="PLH17_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOR20520.1"
                     /translation="MINVTELRPGNHFLDEGVLYSVLDILLNKTAMRKMVAKVKVKNL
                     RTGAILEISRNSGYVVEPVRLDKKKMTYLYDSGDMLVFMDQNTYEQIELPKANMEWER
                     NFLVSNAEIEILAYEDEILGLSLPAKVALQIVECEPAVRGDTVNKAMKDAILETGLKL
                     RVPLFVESGETILVRTDNGQYDGRA"
     gene            complement(27585..28238)
                     /locus_tag="PLH17_01655"
     CDS             complement(27585..28238)
                     /locus_tag="PLH17_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR20521.1"
                     /translation="MLVTLLNESDFVSNTYVLSEDGQAVVIDPGLGDTPAIRDFLVRH
                     GLKLEAVLLTHGHFDHIRGLPYLLETFPDAPVYMHESEVDFLGDSRLNCSYLDSGIPP
                     VSLSIKALGVSDNDALYLLKKDIRIVHTPFHTRGSVCYYIPQEKMIFTGDTLFRSAIG
                     RSDLPTGSPRSIVQSLAKLKSLPPETKVYPGHEKSTTIERELKFNTYLRKEYIPNNK"
     gene            complement(28262..28411)
                     /gene="rpmG"
                     /locus_tag="PLH17_01660"
     CDS             complement(28262..28411)
                     /gene="rpmG"
                     /locus_tag="PLH17_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOR20522.1"
                     /translation="MRDNIILKCTVCGEENYIGSKNKKNHPERREIKKFCSRCNKQTI
                     HKEKK"
BASE COUNT         7672 a         6788 c         6430 g         7585 t
ORIGIN      
        1 atggccaaaa aatatcatac cggccaggtc cgcaagtcag gcgaagacta tattcaccac
       61 ctgattgaag tcggatatat tctcgccaag ctgcaagccg gtccggcaac gattgccgcc
      121 gggtttctgc atgatgttat cgaagatacc cccgccgaat tagaagaggt gcaaagtaag
      181 ttcggagaag atgtcagcaa aatcgtcgaa tctttaacaa agattcagcg catgaaactc
      241 tcacatcgag agtatgaaga tttcgaagcc gaagatcatc gcaaaatctt cctggggatg
      301 gccaaagatg tccgcgtcat catcatcaaa ctcgccgatc gactccataa tatgcgcacc
      361 ctcagcgcct tagaaaataa gcgccagcta gcgttagcgc gtgagactct tgaggttttt
      421 gtgcccattg ctcaccgcct tggcattaac acgataaaaa gcgaactaga agacttatct
      481 ttaaaatacc ttgagccaga caaatataat ttgatatcgg atttacttga taagaaaaca
      541 agaaaccgga cgaaatctct cgactcgctt aaaaagcgcg tcgccgatat cttgtttgaa
      601 gccaaaattc cttttcagat ggaatcacgg cttaaaacga tatattcaat ttataaaaaa
      661 atatatttga agggctataa tttcgacgac atctttgatg tcttggcgat tcgcgtcgtc
      721 actgaaactg agcttaactg ctatgaaatt ctcggcatta ttcacgcgac ttttaagccg
      781 attcccggcc gttttaaaga ctacatcgcc gttccgaaac caaatatgta tcagtcattg
      841 cacacctcaa ttgtctctgg cgatggcaat atttttgaaa ttcagattcg caccaaacgc
      901 atggatgaaa ttgccgaaac cggcatcgcc gcgcactggc gctataaaga aggcacaaac
      961 tataatgcca aagaagagca acggcaaatc gaagaaaaat tgcactggtt tcgcgatttc
     1021 gtcactatct ccaacgagca ggaaggcgat gccaaagagt atatggacac gctttcgaaa
     1081 gatattttcg aagccaacgt ctatgtcttt actccaaagg gaaaagtaat tgacctgcca
     1141 aatggctcaa cgccaatcga tttcgcctac cgcgttcaca cgaaagtcgg cgatgcggcc
     1201 gtcggcgcca tcgtcaacgg ttccctcgtc tcgttaggca ccgtcttaaa aaccggtgat
     1261 gtggtggaga ttcgcacttc gaacacctcg agcggtccta atgaaggatg gctaaaaatc
     1321 gttcgcacag cgcaggctcg ctcgcacatt aaaaagtttt tattaaagaa gaatgcggaa
     1381 ttgatgaaag acgaaagaat tgccaaaggc aaacagtcag ccatcgatgc ctttagggac
     1441 cgcggagtcg aagaggcgga gatgatgacg atgctgaaca atccaaatct tctcaacaat
     1501 ttccatgtta aagatctcga cgaactcttc gtcacgatgt acagcaaaaa tccgacgcct
     1561 tcagcggtca tcgatttttt aaacattcgc cgcaagccga caatcttcaa gctagataaa
     1621 gcgccgaccc caaaagacga tgatcacgtt cccgttttcg tcagcggagc gggcaagatc
     1681 gccatcaatc tcggcagctg ttgcacgccg ataccaggag acgatatcgt tggctatatt
     1741 actaaaggta aaggcgtcac cgttcaccgt gtgacatgcc cgaatatcgc caacgaaaga
     1801 gctcgtctcg tcgatgtttt ttggcgtgaa tcagaacacc ttgacaccta tcccgtcgac
     1861 attaaaatcg agtccgctga tcgccctaat ctcctcatcg atatcatgtc gatgctctcc
     1921 gccaataaaa tctcggtttc gacgattacg gccaagctcc atccgcagac gatgaacacg
     1981 acgatctcag ccacgatttt cgtcagcgac gccaagaggc taaccgatat ttttaatatt
     2041 ttattaaata ttaacagcgt gtatgaaatc acgcgtgtaa tccactgaac agaaatagtt
     2101 ttgcgctgat tttttatcaa atatatttga taaccaatta ttatttgcct tcgagacgca
     2161 attgacactt atagttgttt taaactataa ttaactattg tgtcaaacat agttcaggag
     2221 ggcatttatt tatggaagta aaacccgtta aaggcacgca tgacattatc ggtaatgata
     2281 tcattctcta tcaaagaatc gaagatgccc tcgccaaaat tgcccataca tatggttatt
     2341 tagaattcag aacgccgatt atcgagcaca ccgaactttt cgcccgctcg gtcggtgact
     2401 caagcgacat cgtccgcaag gagatgtaca cttttctcga taaaggaaat cgttcgctca
     2461 cccttcgccc cgaagtgacg gccagcatca tccgcacaat cgtcaacaac aaattatatt
     2521 catctcagga ttttccgatt aaagccagct acctcggtcc gacctttcgc tatgagaggc
     2581 cccaagccgg aagataccgt caattcagcc agttcggcgt cgaagcggtg ggtcttgatt
     2641 cccatctgat cgatgtcgaa gtcatcgctt tgggctataa cgctcttaag aacttgaatt
     2701 ttaaagatat cactctaaaa attaatacct tgggcgatga tgcctcgcgc aatcaatatc
     2761 gcgaagcgct tcgcggctat tttgccggcc acatccacga gatgtgcatc gactgccaag
     2821 aacgcctcaa cagcaatccc ctgcgcattc tcgactgcaa agtcgaagaa gaccgcaaaa
     2881 tcatcaaaaa cgctccgcgc attcaggatt ttttgtcgga gcaatccaaa gagcgcttta
     2941 gtaagacgat taacctgctc gattctttaa atatcaagtt tgacctcgac gagactctcg
     3001 tccgcggtct cgattactat tcagaagtcg tttttgaata tcactatatt gctccggatg
     3061 gtaaaaccat cggcgccctc ggcgccggcg ggcactactc gcatcttgtc gaagagctcg
     3121 gcggtccgaa tttggccggt gtcggcctct cttttggcgt cgaacggctc gcccaggttg
     3181 tcgccagcga aaatttactt tttgatgcct ttccgtttct ggctttctac atcatgccca
     3241 tcggcgaggt caatatcgaa aaagtctatg aactaaacga gaaaatccat cgcctcggtt
     3301 atccttccga gatggcttat gaaagcaaag ctttaaaggc cttgtttaag aaggcggaaa
     3361 agcgctcggc cctctttgcc atcctgatcg gcgaggaaga gctagaaaaa gagatgctga
     3421 cgattaaaaa tctccgcacc aaagagcagg cttcggtcgc tctggccgat tttgacagcg
     3481 tcgccaacca aatggttgaa gaagaactca tcgaattaaa cgaagagagg taacccatgg
     3541 aaagaacatg ctataacgca agtctatcaa ttaacgatgt cggtcgcgaa gtcaccctat
     3601 tagggtgggt tagcaagcgc cgcaatttag gaagcctcct ttttatcgac ttgcgcgatc
     3661 gcagcggtat cattcaggtc accgtcaaag acgatgtcaa agttcctgat gtccgcaacg
     3721 aatatgtcat cgaggtcaaa ggtcgagtcg ccaagaaaga tgtggccaat cctaaactca
     3781 aaaccggtga aattgaaatc atcgccagcg caataacaat catcaatgaa gcggccacga
     3841 ctccgctcat catcgatgat gacaccgacg ccttagaaga tgtccgcctg aaatatcgct
     3901 atctcgattt acgtcgcccc ctcatgcagg aaaaacttaa agtcagggct aaaatcgtca
     3961 gggccgctca cgagttccta gatgataacg acttcattga agtcgagacg ccaatattaa
     4021 ctctttctac ccctgaaggc gcccgcgatt atgtcgtccc ctcaagaatc catcatggat
     4081 gtttttacgc cttgccgcag tcgccgcagc tctttaaaca gctgctgatg atcggcggat
     4141 tggaacgcta ttatcaaatc gcccgctgct ttcgcgatga ggacttaaga gctgaccgcc
     4201 aacccgattt tacgcagatt gatatcgaga cgagttttct tgatgaaacg caaattctga
     4261 ccttgatgga aggccttctt aagaagatat tcaaggatgt cattaattat gacctcagat
     4321 tgccacttcg aagattgagc tacgatgaag ccctgaaccg cttcggaagc gacaagcctg
     4381 acacgcgctt tggcctcgaa ctaaaagacg taaaagacct tgtcagcaag acaccatttc
     4441 cactttttaa tagcagtgca catagcaagg ccatcgtcat ccccaacctc gccgaaacaa
     4501 cctcgcgcaa aaatatcgat gaactgcagc ttgaagccaa gaaattccgc ctttcgagct
     4561 tcatcgtctt aaaagttaaa gatgaacacc tcgaaggaag cgccgccaaa ttctttgcgg
     4621 aagaggatgg ccgcgcttta atcaaaagcc tttctgcaaa acataatgat ttaatcatta
     4681 tcgcctgttc aaactcactg gccaatgtat gtttcggcct cggcgccttg cgcagccgct
     4741 atgctcgcga actcggcctg attaaaccga acacctatga cttgctctgg gtcgttgatt
     4801 tccccctatt tgaaaaagat caagatagcg ggaaattaag ccccctccat catccgttta
     4861 cccgtccgac agcggcgacg gccgagtggc tatcaagtga tcccgcccga gttcacgctt
     4921 cagcttatga cattgtcata aatggctatg aagccggtgg tggctcgctt cgcatctatg
     4981 atcaaaagat gcagaagact atttttgaag tcctcggttt cagccaggag gacatcaaga
     5041 ataagttcgg tttctttatc gatgccttta aatacggaac gccacctcat ggcggtatcg
     5101 cctttggcct cgaccgcctg acgatgattc tcagcggcac cgacaatatc cgcgatgtca
     5161 tcgctttccc caagaatctg cgcgcttcct gcccgatgac cgacgcccct tcaaagatcg
     5221 atcaagctca attagatgag ttgggaatta agtttgtcga ataatttatt actaagtaat
     5281 aattaaacta aatatatact aaaaaaatac taaggagaaa tcaatgaaaa ccatcgacaa
     5341 gacaaatctc gaccagttag ccgtcagcgc cattagggcg atgtgcatcg acataacaaa
     5401 taaggctaaa agcggccacc ccggcatgcc tctgggcagt gctcccattc tttatacttt
     5461 gtataagaat catcttatcg ccaatcccca cgacccgaac tggattaacc gcgatcgctt
     5521 cgttttaagc gccggtcacg ccagtgcgct tttgtacgcg atgcttcacg tcaccggttt
     5581 tgacttaagc atcgatgaac ttaaggcttt ccgccagatt gattcactca ctcccggaca
     5641 tccggaatat cgccacacca aaggtgttga agctacgacc ggtccgctgg gacaaggcat
     5701 cgcccaggcc gtcggcatcg ctctcgccga gcaggctgtt caagcccagc atgtcgaggg
     5761 acacaaactc atcaatcatt atacgtactg cctctgcggc gatggctgcc tgcaagaagg
     5821 aatcagccaa gaagcaattt catttgccgg tcatcaacgt ttaaataaat taatcttaat
     5881 ttacgatgcc aataaaacga ctctggatgc ccctcttgaa gcctcgtttt ccgaaaatgt
     5941 cgaattgcgc ttcttggctt cgaactggaa tgttctcaag gtcaaagacg gcaacgacct
     6001 cgatgcgctt gatcgcgcga ttagcaaagc taagaaatca agcgattatc cgactctcat
     6061 catcgtcaat acaatcatcg gatatggttc ttcaaagcaa gggacggcga aggttcacgg
     6121 caatcccctg ggcgttgaag acggacagaa agccaaagcc ttttacggct acgattatcc
     6181 cgatttcacg attccggaag aagtctatca agttttccgc gacacatttg aaaagcgcgg
     6241 catcgaagaa tatcagaaat ggaaaaatgt ccgcgacacc tataaggaca agcatcctga
     6301 agagtaccgc gtctttgaac acgcctttaa aggcgatgtc accaactata tctataattc
     6361 gattccgaaa gtcgaagccg gaagctctga ttcaacgcgc ggaatcagcg gacatattct
     6421 caatgaactc cacaacacga ttccctttat cgcgggcggc tcagctgatg tcgccagcag
     6481 cgtcatgacc aagattaata aaggctgtga cttcactccc gcctgccgcg aaggaagaaa
     6541 tattaacttt ggcattcgcg agtttgcgat ggcggccatt caaaacggaa tgctcctcca
     6601 tggaggactc aagacgtatg tcggctcttt cctcgtcttc agcgattatt taaaacccgc
     6661 cgtccgtctg gcggcgctat cacgcctgcc ggctatctat cttttctcac acgattcgat
     6721 cgctcttggc gaagatggcc ccacccatca gccaattgag cagttggcaa tgctcagaag
     6781 cattccgaat ttacgcgtaa ttcgcccttg cgacaatgcc gaaacatatg gcgcctggcg
     6841 catagcgctt aaatccaaag agactccgac agccatcatc ctatcccgac agggccttcc
     6901 tgaaatggaa ggttcgacga tggaagcggt ggccaaaggc ggctacatca tctctcccga
     6961 aaaagaaaaa ctgatggcga cgattatcgc caccggaagc gaagtcgccc tcgccgtcaa
     7021 agcccagaaa cttctcctgg acaggaaaat tgatgtccgc gtcgtctcga tgccttccat
     7081 cggcttattt gaagcccaag atgctgaata caagaagaac acaatcggtg tcaccaagga
     7141 caaagtggtg gccgtcgaaa tgttaacatc ctttggatgg tatcgctatg ccgacaccgt
     7201 gatgggcatt gatacattcg gagcgagcgc gccgatggct gatgtcgtca agaaatttga
     7261 cttcacgagc gagcggctcg tcagcctcgt cgaaaaaatt gccaagtagt gataaacttg
     7321 ctaaaaagac cgcgattatt taagataatc acgacggagg gagcatatgg ctgattacgt
     7381 ctacattgaa aactattcca aatatggagt gatgggtatc tcccgccgcg tttttgaaac
     7441 gattgcccag acggcgacca atcgcgtcaa gggagccact gtctccgcaa acaaatcgcg
     7501 ccttttcacc ttgcatcgcc cgattcaagc cattttgcgt agcaacggtc ttgtcgacat
     7561 ccgcattgat gtcgccatta aaagcggtgt caatgtcagc gaggtgtgcc tcaaaattca
     7621 agaagaagtc gccaactcct tgatggcgat gacggaaatg gttccgttta atattaatat
     7681 taaggtcgct tccattcaat aggctctcac gagtctattt ttaaattatg aaatcaaata
     7741 aaccccagcc cacctatgcc gaagcgcaca ataaagcggt gcgcctctat cgcaagaccg
     7801 ccattttttt aatgtgggcc ggaatcatat cgatcctcgc gacggttatc gccctcttta
     7861 aaagcgaagc gggttatggc atgtcgctgg ccattaatat ttttctcaat cgattgattc
     7921 acgctctcgg gctttctaac ttagccactt cgcttttcat ctttgctctt tcggtggtta
     7981 ccggcgccct ctttgccgtc ctcggctttt tcgcccagca aggcaaaaaa atattcctgt
     8041 tcatcggcgc tgccctctat ttagctgatt ttgccgccgt attcctcgtc tacggggcga
     8101 attggggcac gacctatgct tttaccatcg cgactcattt ggtcatcctg gtggctcttg
     8161 ctattgccat ctatgaatac ttcaacgtca tcgccatcga gaaacgtttt cacggcgcgg
     8221 ccactcctaa cctcgacgag gcgaacggaa cggaataata tgagcacaaa taatacccgc
     8281 aaccaaaatc agattctcgc catgtcgtgc atttacgatt atttgacgag ggtggagatg
     8341 ggcgaagata tcgatgtcaa agctcttctt gaacgcgttt gcgatgcgcc gtttgccgat
     8401 atcgacacat ttgtgtcaga agtatttatt aaagtggtta gaaatcacga agaaattgtt
     8461 gcgatgttac aggcgaacat gaataaatgg aaattttccc gtttaaatcg catcgcccaa
     8521 tccattttgc tcttatcggt cgctcactat cgctatgtcg gcgaagtcga tcgcagcgcc
     8581 gtcatcgaca tcgccgtcag aatggccaaa cgctatcttg ataatgccga ctataaattt
     8641 attaacgccg tcctcgacag caccttatga aaagcggaga attttatacc gtcacgagtt
     8701 taaatcgcca tatcaaagaa attcttgatg gcgaagtttc actccgctta gtaaaactaa
     8761 aaggtgaagt atccaatcta aaaaaatatc cgagcgggca tttttacttc accctcaaag
     8821 acgagaacag cgtcatctcg gccgtcatgt tttataatga tgcgattcgc atcggcttta
     8881 atatgaaaga cggcgacgaa gtcttagcct atggctccgt cagcgtctat ccccaaaggg
     8941 gcagctatca gatttacgtg agcgaaattg aattattcgg tcaaggcgcc ctgctggtgg
     9001 aactcgagaa gttaaaacag aaattaaagg ccgaaggtct ttttgacgaa aatcgcaaac
     9061 gcccgatcgt ctcctatccc gagtcaatcg gcatcatatc ggcaccggga agcgcggcga
     9121 ttgaagatat gatcaagaat attcatcgcc gttatcccat cgccagaatt tatttctttc
     9181 cgagtttagt gcagggaagc accgccccca aggatttatt gcgagccttt gaactgacta
     9241 aatcatatga tttaagcacg cttattatcg gccgcggtgg cggggcgagc gaggatctca
     9301 atgcctttaa cgatgaggtg ctagttcgcg ccttagcgac gagccgggct cccatcatct
     9361 ccgccatcgg tcatgaaatt gattttacct tggtcgacta tgtcgccgac cgccgcgtct
     9421 cgaccccgac gggggccgcc gagctggcga cgcctgacaa acgcgagatt tatgaaacac
     9481 ttaacgaaaa tcttgaaagg atgagccttg ctttgcaaaa taaggtccac actctaaaag
     9541 agaggctcaa cctcctggca aagagagctt tcttcatcga tcccaaatcc atctatgaaa
     9601 ataaagtccg cgatgtcagg gaatatcgcg ttcgccttgt caatgcgatg caaaacagtt
     9661 atcttatcgc tagccacgag gtcagatcat caaagcagcg tctcgaagcg ctgtcgcctt
     9721 ttggcgtctt aaagcgcggt tactcgatca tgaccgatga aagcggcaag gtaatcgact
     9781 cagtcaacaa agttgaaaca ggtcaaaacg tcttgacaaa cgtcaaagat ggtataatta
     9841 tgtctcgggt taccgcaaag gagagcaaga aaaatggcta aggaaaatga aatcaatttt
     9901 gagaagatgt tagctcgtct tgaagaaatc gttgcgacga ttgaaagcaa aactctcccc
     9961 ctcgacgaaa gcatcgccct ttatcaagag ggcaaaagca ttatcaaaac gctggaagaa
    10021 gcgcttaagg atgccgaagg taaaatcgcc gatattttaa aagttgaata aattcaaata
    10081 taaatcatag tataaaatta gtatataaat agtatggcaa aaccaagtag caaaaggata
    10141 attgacatcg acatcaggga tattaaaggt ccggaatttt taaaggagct caattatcgc
    10201 tcgctcgatc tcctctcgga aaagattcgc cgcgagatca ttgataagac cagcaaatac
    10261 ggtggtcacc tttcaagcaa tctcggcgtg gtggaaatca cgattgccct tcatcgcatg
    10321 tttaatttta aacatgataa gctgatattt gatgtcggcc accaatcata cacgcacaag
    10381 attctcaccg gtcgctccct tgaatacctg cggaaccgca atggcgtttc cggatttcag
    10441 aagatttgcg agtcttatta cgattgtttt gaagccgggc atagttcaac ttctctaagc
    10501 gccgcgcaag gctttgcctt aaatcgcgat gccagaggtg aaaaatacga tgttgtcgcc
    10561 gtcatcggcg atggctcgat cgtcaacggt ttggcgtttg aggccctcaa taatatttcg
    10621 cataacaaca ataaggttat tatcatttta aatgataatg agatgtcgat atcccgtccg
    10681 accggcggca tgggcaggtt ctttgctaaa atctcgacgg cttcaggtta taccaatttt
    10741 aaaatctcct accgtcgttt tatgtttaaa acgcggatgg gacacacact ttacaatttt
    10801 atctcgcgca ttaaaaatgc cattaagcag aagcttgttc cgacgactta ctttgatgat
    10861 atgggctttc aatacatcgg ctgtatcgac ggccataaca ttaaagccct cgagaaagcg
    10921 attgcgcgag ctaaaagaac ccctaaatcg gtcgttttgc atgttgcaac agttaagggt
    10981 aaaggctatc gatacgccga aaatgatacg accggttact ggcacggcgt gacgcctttt
    11041 gaaatcgaga gcggaaaacc gaaaaacctc catcccgaag agctgtcctg gtcgcatctt
    11101 ttcagcgatt tgttaaccgc gctgatggct gagcgcaatg atttagtcat tatttccccg
    11161 gcaaccgtta aaggcagcgg tctcgaagac cttttcagca cctatccgaa aaggacattt
    11221 gatgtcggca ttgccgaagg ccacgccctc acacttgcaa gcgggctcgc tgtcgccggc
    11281 acgcatccga tcgtctccat ctactcgact tttatgcagc gagcttttga tcagattatc
    11341 catgatattg ccaggatgaa cttaaacatt actttcttgg tcgatcgctc cggcttggtc
    11401 ggattagacg gcgaaacaca ccagggcatc tatgatgaat catttttgct gagcacgccg
    11461 aatacggtgg tgacgatgcc gtcaagcgat aaagaagcga agtatctgct caacgaatct
    11521 ttaaaacaac acggaccctt cttcatccgc tatccgcgtg atcttcttaa gaagaaccat
    11581 ctgattcaag aggaaaatca tcaatttggc aaatggatta tggaatgtgt tgccaactcg
    11641 aaacgcgtcg ccgtcatcgc cgtcggccag ctcctgagaa gcctcaaagg actcatcatt
    11701 gaagccgatc tcgatgtcac tttagtaaac gccttatttt taaaacctca tgatcaggag
    11761 atgttaagct ctttaagcga atatgagacg attgtcatct atgatgccta tgccaccgaa
    11821 ttcggttttg cttcctactt aaaaacaaag ctttgcgaga tgtcatacaa aggccagatt
    11881 aagtctttcg ccattcctga tgtcttcgtc acccaggcaa aaatcagcga gcagcttgaa
    11941 gaattgggtt tattaccaaa gcaagtgttc gaagaaataa agaaacttct ttaaagaaaa
    12001 tacagaaact ggttaacata atgttgttat ccagttttta attatttaca atataattga
    12061 tgagcaagat tatcgtccac atcgatttaa acgccttctt tgcgaccgcc gaagaaattc
    12121 gcgatccgtc tttagtcgat aaacccgtca tcatcggagg cgacggtcgc aaaggcatcg
    12181 tctcgacagc gtcttatgcc gcccgcaaat tcggcattcg ctccggcatg ccgacatttc
    12241 aagccaagcg actatgccct catgtcctca tccttcccgt cgatttccgc tactatggtt
    12301 tgctatcccg cgagtttttc gccttcgtcc gcaaatacag caagaaagtg gaaaaagcat
    12361 cggtggacga atgctatatc gatatgacag aggcccttga aaatgtcgaa gatgtgcgcg
    12421 cctatttttt aaatatgcaa aacaaactct tagagaagac cggcttaaag tgttcgattg
    12481 gggtcggacc gacgaagttt ttggccaaga tggcctctga tatgaaaaag ccgatgggat
    12541 taaccatttt aaggcgaaga gatattcccc gactgctcta tccgctttcg attcgcgact
    12601 tttatggcat cgggcgcaaa acttcgccta aactggaagc gatcggcatc aaaacgatcg
    12661 gcgatttagc taggcgcatc aatagcgatg accctgagat gcacctcctg ctcggcaaat
    12721 tcttcttcgt catcaaagat tgggtcaacg gctacggaaa tgacgaagtc gatctcgaac
    12781 cgtgggaccc gaaatcaatc ggtaacagct cgacttttat gcatgacacg gacgacttta
    12841 tggaaataaa agagatgatt tcgacgctcg cgaaggaagt cagtgaacgc gcccaaaatg
    12901 agcgaaagca aggttcgacg gttcagctgg ttattaaaga tacggaattt aagacgatta
    12961 atcgctcgct tacgttcgcg cgaccaacca atgaatacga agttattttt gataaggctc
    13021 taactttgtt agaaaggcat tacaaaggcc agccgattcg cctcgtcggc gtcacgctgc
    13081 aaaacttgat tgacccgcgc gatctcgtca ttcagatgtc gcttttcgac tatcgaaaac
    13141 acgaggaaga aagtgagaca aaactattaa ttaacgagtt gaaccggaag ctaaaaaagc
    13201 cgatgctcat ccgcgccagc gaggtcgaac acgacaagag aggaaacaaa gatgaagatt
    13261 gatgatgcga tcgatttatt ctttcagcac ttaacggttg aaaaaggcgt tcagccccag
    13321 accattcagg cctatgccaa cgatctaaaa caatttttca aggtgtttaa agacaaggag
    13381 tctaccgatg atctgctcgt cagcgatctg actgacttta tcaagatcca gagcaagcaa
    13441 aatcgggcca atgccaccat tttgcggcgg ctctcctcga ccaagcattt ttatcgcttt
    13501 ctagaaaaag aaaaactgct ccccgatgcg gtgcccaaga tcgaatcgcc gcgggccatc
    13561 aaaaaacttc ctctgtcgct tagtgtcgaa caagtcgaat cgctccttga agccccggat
    13621 cttgaaaagc cagagggcat gcgcgatcgc gcgatgctcg aagtgatgta ctcatcggga
    13681 ctccgcgtca gcgaactcat caatttggag cgctcccaag taaactttac ccacggcatt
    13741 gttaccattt atggtaaagg caataaagaa cggcgcgtgc ccatcggcga atacgccttg
    13801 gaatatctgg tccattacat cgaagaagca agaagcaaaa atatcggaaa gaatacgaaa
    13861 tatctctttt taaaccgcta cggaaaagag ctgtcccgcc aatatttttt tcggcaagtg
    13921 aaaaagtacg ccacctatgc gggcatcaaa gaagaaatat caccccatac actgcgtcac
    13981 tgttttgcga cgcatatgct cgataaaggc gcggagttaa gggcggttca agagatgctc
    14041 gggcaccaga atatcgcgac cacgcaaatc tacacgcatg tctccagcaa ccgcatcctg
    14101 agtgcgtatg atttgtatgc taagcgcaaa taatatacaa tctttatgtt aataaaggag
    14161 taaccatgaa taaatataaa agaatgttcg tcatcgtcat cgattccgtc ggaatcggcg
    14221 agatgccgga tgctgagaaa tttggcgaca aaggggccaa taccctcgtc cacacagccc
    14281 gcgaagccgg tggtttaaaa gttccggtca tgaattcgct aggcctcggt gacttagcgc
    14341 cgattttagg caccagcatc gtcaaacacc cgcattcgta tgcccttcgc ctccgcgaga
    14401 caagcgccgg caaggatacg atgaccggtc attgggagat gatgggcatc aatacgacca
    14461 aaccctttca gacgtttacc gacaccggct ttccccaatc tctgattgat gaactggccg
    14521 agaagaccgg acacaaaatc atcggcaaca aagcggccag cggaacggag attctcgtcg
    14581 aattaggcga gcagcaaatg cgcgaaaatt ccctgattgt ctatacttcc gccgatagcg
    14641 tcttacaaat tgccgcccac gaagaagtga ccggcgtcaa agaattatat cgttgttgcg
    14701 atatcgcccg cgagatatgc atgaagccgg aatattttgt cggccgcatt atcgcccgcc
    14761 cgtttatcgg cactaataaa cacaatttta agcggacggc caaccgtcat gatttagccg
    14821 tctcgccgac cggaacaacg accttagacg ttttgaaaaa caatggtttt atggtcagtt
    14881 caatcggcaa gattaatgac atcttcaata cgatgggtgt cgtcaaagcc cagaagacgg
    14941 tctccaacct cgacggtatg aacaaaacga tcgaagaagc caagaatcac gattttaccg
    15001 gattatgtta tgttaaccta gtcgaattcg acagcgagtt tggccatcgc cgcaacgcca
    15061 ttggttatgc caaagctcta gaagagttcg atgttaaatt aggcgagtta ttaaaagttc
    15121 ttaaggacga tgatttggtc atggtcacag ccgaccacgg caatgatccc gtccatcatg
    15181 gcaccgatca caccagagaa aaagttccgc ttctaatcta ctcaaaatcg attaaaaacg
    15241 gccgctatct tgatgagcgc gaaactttcg ccgtcatcgg agcgagcgtt ttagaaaact
    15301 ttggacttaa gaagacgccg aatcaaatcg gtgcccccat catggagatt tttgaataaa
    15361 tgaaaacaaa aagtattatc ctgcccctag cagccatcac cttgagcgcc tgtggcggac
    15421 ccgccccaga aatcacaatc cgcacgattt ccccggccgg cgccccgacc ctggcttttt
    15481 atgaccaggg tagcaacgag aacttcgtta caaactcgac gccgagcaat gttttagccg
    15541 aactgcagac caattactat aacgcggtcg tcttcgattc gataaatgcc ttaaaaagca
    15601 ttaaaaataa tgatcttgac tttaaattag caaagctaat aacgggcggt aacttctatt
    15661 tggccagcat taataaaaac gaaggcgaaa tgccgaccgc cgatgatgtc gtcgtctctt
    15721 ttggcgaaaa cctcattccg gatctcgtct atcgcaaatt gtcagaatac tgggaaattg
    15781 aaaacacgcc atactacgtt ccaagcgtgt ccgaggctct cggcgtctta aagaccggcg
    15841 tctacgcaag taatcccgtc gattttgtct ttatcgccca gccggccctt aacatcgccc
    15901 taaatgatgc gacggccgcc acctacggca atgtcaaagt cgttaaaaac attcgttctg
    15961 aatggcaaga atataccggg cagagcgcca ttccgcaagc cggtttattt gttcgcgcca
    16021 gccactatga attaaatccg accgtttttg aaaactatgt cgatgagatg gtcgcaagaa
    16081 ttgatgtcgc tatcgacacg ccggcgacgg ccaaagccgc cctcgacgcc tatggcgacg
    16141 cccaggctca agctgctaaa ttcggattta actcaaatat tatgttaacc acacaaagcg
    16201 acggggccaa cggcttcggt ttagttcgtg gcaccgaaga agtcgatgtc aacgaatttc
    16261 ttgaaacgct cggattagaa acatttgctg attcctactt cctcggtttg taggtaaaat
    16321 tataaatgat gaataaaacg aaagccttct tccaaaaaca cggcgcagtt atttatacgg
    16381 tgcttggcat tggcttcgtt tttcttttgt ggtggctgac ctcgctcatc gtcaaagcga
    16441 ccgtcttgcc gacatttccc ggcccggaag atgctatccc cctctttttt acgctgtttg
    16501 gcgaggccac aacctggctt gccgtcggcg gaaccctgct tcgtttagtt atcagctttg
    16561 ccatcgcctt tcttctcggc tcgctaatgg gcgtcatcgc cggcgtgcac aaaagcttcg
    16621 cccattttat gcgaccgctc gtcattactt taaggacgct tccgaccgcg gccgtcattt
    16681 taattctgat cgtattgctc cgtccccttt tcgcgccgat catcatcgtc tttttaatta
    16741 tctttccgat ttcttatgaa tcgatggcaa ccggaattaa aaacgtcaac cgcgaagtgc
    16801 gggaagccgc taaaattgac ggtgcgactc gcctccagtc catcatccgc attaaaatac
    16861 cgctttcgat gccctatgtc ctcctgggaa tcgtctcaag cctcggcctc ggcatgaaaa
    16921 tatcgatcat gtcggaaatt atcgccggct caaatcgcat ctccggactc ggacggttga
    16981 ttcacttagc ttacatcgaa gctgacatga cgagaattgt cgcgatttca atcttcgcga
    17041 ttattctgat cggactgatt gacctcggca tgtacttcgc gaaaaaaaaa ctcaaaggcc
    17101 gttaattatt atcatttcgc aaaggcgcta aaagatcgta tgatcgcgcc gatgagcaaa
    17161 atagatgttc ctcgcttcgg ccagggactt tttatttggg ccgcttgcaa ggcgaggatt
    17221 tgcttcctga gacgcatttt taacccatcc gcaaatcggg caaatctcgc catcaaggcc
    17281 cttgccaaga gtgaattctc cacagcatga acagcatctt gtcgccataa aaccattata
    17341 aaacttatga gaataaatat acacgtaggg tttttaatat tgtggcggta ttattcaatt
    17401 agtagtaatt tagtatttta ttataacggg cattatgtgg aaaactcctg acaatagata
    17461 atttaataga aatgtattaa ggaaaagaaa tgagaaaaac accgataaaa tctataaaaa
    17521 gtactatatg gttaacataa tggacattat gcgaagttaa tcatatttcg cgaatcggac
    17581 cagtttgggc aaaaatcgct gctaccacta tttatgctct cgataaaaaa agaaccgtga
    17641 atttgattcc ggttcaaaat gtgatcttta aaggttaacg ccgagacaag atctgctcga
    17701 ggctaattct ctcacgttct tcctttgaga agatattgat gatgacatga taggcatcaa
    17761 ctaatatcca tcccgaattc tcgttgcctt ccacgtgatt gattttacct ttgagcgttt
    17821 cgatcttttc gacaaccgct tctttaagag cattcatctg ccgcggattg gtggccgtga
    17881 cgagaatgta gtattcgctg aatggtgtct tatcgcgaac gtcgatgatt tgaatgtctt
    17941 cagctttcgt ctcttctaac agctggagga tgcccttggt gtacttatct gaattcaatg
    18001 tctttcccct ttcctaggta aagctgcata caggcgtcag ttagcggatt attaatgtct
    18061 ttgccgttgg cgagaagata ctcgcgattg gcacttaaga ccactacaaa acccaagtaa
    18121 tagttttgat agcatttttt gatgagcggt ctcgagttaa agcctcgcag aggatcgatc
    18181 ttgtcggcgc agtagataat cttgccaagc gggctcatgt gcggcttgcc ggtcgcgtga
    18241 aagcgaatcg cgtcaaggac ctcttcgtct ttgatgccga attccttttc ggccaaatat
    18301 gatccgacaa actgatggta ggaaaactca ggttggttga cataatcttt atattgtctt
    18361 ttcatgatgg caattgtctg cgctgtgtca taacctttgc cgagatcatg aagcaagccg
    18421 gcaatgtaag ctttcggata atcattccgc tcgtttttat aagcgattga acgcgcgagg
    18481 tgagcgactg aaagcgaatg ttcgaaacgt tccttgtcga gcattccggc gatcttctct
    18541 atataataga ggcggttttt ttcgatgtaa tcaagcaccg ctgaaggcgt gtcgacagat
    18601 tgcaaattgc gaattttggt cgaggagact tcgccggtat gattatacgg caaactcata
    18661 attttatact ttgtcacatt accttcattt attttgatgt cagggcgcgg tatgtagacc
    18721 accgtcagca atttaacaat ctcctcaggt tcccgccact ggtgaaactg attgacctga
    18781 tcggcgccga ttaaaaggta aaattgtttc ttgggatatt tatttacaaa gtaacgaacc
    18841 gtatcaatcg tgtaattgac ttcgacctcg gaacggatct cgtaatcgga aatcgtcgtc
    18901 cccgacaatc cattttgacg aatggctaaa gccagcattt tgagccggtg atcaatgccg
    18961 acactcggat ttttccatcg cgggcttttg ctgggtatga agatgacatc ggcgttgagc
    19021 aaaaaactgg ccgctcccgc cattctgaga tggccgttat ggaccgggtc gaaagctccg
    19081 ccgaagagaa tgacgctttc cactattttt tgttcctctg cggtttttgc cgatataaga
    19141 caattgtctt accaattatc tttatcagct cacaatcaag ttttgcgcag attgccgtgg
    19201 cgaaataatc agcttcctga ctggcgtttt tattgactga caccttgatc agttcgttgg
    19261 ccaaaagagc tttatcaagc atttctaaaa ctttctcgtc aagattatcc ttgccgatga
    19321 tgtagcgtgt tttaagcctg ttgctcaaag caaataattc ttgcctgtcc ttgttactta
    19381 acatcttgga ttttagacct cgtcgtgtag aacgaaaccc ctttggggag gtagaggcgg
    19441 aaggtttgat tggcgccgat aaaagagaac cagcccagac cgtttatgcc gatgtcgcgc
    19501 gagccttctt cgtcgacgac gatgtcgtag atgtcaaaat ctttcaggct ctgcaaattt
    19561 ttcgaagtcg gatggagcaa gtctttgcta agtgttctta agaactgctt gttggcatcc
    19621 ggcaagacaa tttttttgat gtcaacatca tcagaaaaat agcatcccac cgccgttttc
    19681 tcgccttcga ccaactcgat gcgggccaga cctccgatga aaagagactg cgtttttgtg
    19741 atggtgaagc gccgtttttc aatctttttg acgggaacga ttgctttcaa gactggcttt
    19801 tcgacctttt cgagaacaga attatcgttt cctaatccgg gcgtgtcata aaaatatgtc
    19861 gattggtcga ttggtatttg catgacttta aggtttgtcc ccggataatt ttgagtgatg
    19921 atattcgtcc ggcttaagtt cttatattcc ttaaggaagg cttccatcaa agtcgttttg
    19981 ccagagtgtt tctgaccgat gatataaaca tcgcggcgcc ggcgcaaatc cttgatgaca
    20041 tcaatcacat cgcgaatgtt gtagttggcg gaagcgctca ccaatttgac atcgaggact
    20101 tttaattttt cgactctgag gcgatgggcg acatattctt tgagttgctc atcgtcgata
    20161 tcatcgggaa ggatatcgcg cttattggca agcacgagga tatcaaggcc cgaaagccaa
    20221 tggttgaggg cgcggataaa accggcctca aaagagaaga ggttgatgac ataaacgatt
    20281 aaggcatcgg tggcctgggc atcggcgatg agggtgaaaa attcgtgatc gagactcgcc
    20341 tcgcgcggtg cgagattata tttctcgctg tggaaacact ttgagcaaaa aaggacacct
    20401 tttgacaggt cttgcagaaa ttcattttcg acatagccgt ctttttgcgg atccttgctt
    20461 tgcaagacga ctccgcacga gtagcagcgg cgaataatat ttaacttagc cattatcttt
    20521 cctcccagct gattaagaga ttttgttttt tcattttttg atagcgcgga gcatcaaacc
    20581 agcgattaaa acgcgtcgtc cactggtcgt cttcgacgag cctttcggtc agaataacgc
    20641 gaatgccggc gccatttccg cacgccacat cggtgatgat ttgatcgccg atcatcatga
    20701 ctttggaaat atcgatacca tttttcttaa taaactttct aagtttaaaa gcaaatggtt
    20761 tatatagtga tccggcatag cgaaccccta attctttagc atacgtcgaa acccttttcg
    20821 aagtattgtt ggagacgacg atcatctcga ttgcggcctt gctcagcttt tcttttaagg
    20881 caaaagcttt gtcggtcgga tgcgactgat ggtaagcatc taatgtgtta tccaagtcga
    20941 caaacaaaat cgatatatta tgtttttgga aaaaggcggg ctcgatgtcg taaatgctct
    21001 tagccacgca gaagggaatg aagcgtttat tcatattctt attttacaaa ttataattgt
    21061 aaaaacatag ataaatacca taaaaataag aaaaaaaggc ggcgtcagat atttggcgcc
    21121 gccagttaaa agcacgaaat acgcaagaaa aactattttt tcgtttcgtc ttcttcaaaa
    21181 atggagatct gtttgtactc ttcgccgttt tcatcgagtt caacgccatt agaaacggcg
    21241 atcggttttt ccttgatggg cggttcgtga gcgataatcg tctgatcgat catgatgtcg
    21301 tcttgccgaa agacaatcgc taagtgcgag cgccgcggca aggtgatcgt gcccttggcg
    21361 tatttgttgc gcggagtgac atagaattct tccactggca cctcgatgat cgctttgtta
    21421 ttggcttgca ctcgccacac aattttttca tccttagcgc ttattttctg agcgaaaatc
    21481 aaataatgag gctcgttttt aaagcacgga tagattggtg tcgtcttgcc taaacgaacg
    21541 gttaaaggcg tattggcata atcataaatc cgaatgtgtc cagcatgcgt aagcaaggca
    21601 attttggctt tttcgtcttt ttcaaagacg agtattttag caatcaccgc cggttttaat
    21661 tcggcgatgg ccttgacgcc gccggatttg agggtggcga ttgacacatc gttttcgtta
    21721 aagaggattg cgcctcccgt gctggtgagg acgaggaggt tgctgtcgcc gcttgttacc
    21781 accacatcgg caatttcatc atcaccgatg agcttcatcg ctgtaatcgg tcgcgcgtag
    21841 cgaatgacga caaaatcgct cagacgcacg cgtttaattt ggcctttttt gctcacgagg
    21901 gccaaaaaga gatctccgcg gaattcattg acggcaaaag cgcagacgat tttctctttg
    21961 tactcgaggc tgatcaggta attgatgtgc ttgccttcct ctttccattt cgtatcgatg
    22021 atttcgtgaa tcggaatgta aagatagttg ccgcgatttg taaaagccaa gacaaaatcg
    22081 gtcgtcaccg cctggccccg gtaaacgaga atatcatcct ctttcattcc ggggagcgaa
    22141 tcgacgccgc tgccgcgata tgacttcacg gaactgcgct tgatgtagcc gtcgcgagtg
    22201 atggcgatca tcacgtcttc cttggcaatt aagtcgcgtt tgtcgatttg caggttaata
    22261 tctttttcga cgatttgggt tcggcgatca tctccgtatt tggcggcgat cgcccttaga
    22321 tcagcgatga tgacgcggtc gagttttttg cgatcctcaa ggataccttg aagttttaaa
    22381 atcgtctttt cgaggctttc tttttctttg acgagcgtgg aaatatcggt attcgacaac
    22441 ttataaagtt gtaacatgac aatcgcttcg ctctgcgctt cggaaaagtc gaacttcttc
    22501 tgcaagtttt ctttggcgct ggtcttatct ttggaagcac ggataacctc gacgatttta
    22561 tcgagtatcg agatggcttt gatgagtccg tcaacgatgt gcagacgagc ttgagacttg
    22621 tctaagtcga agtggctgcg ccgcgtgata acctcgactt ggtggtcgat ataggcatcg
    22681 acaatttgca gtaaattaag cgttttagga cgcccgttaa caatggcaac tatgttggcc
    22741 gaatacgaac ttttgagttg cgttttatta aaaatataag ccaagatgac ttctggttta
    22801 gcgtcttttt tgagatcgat gacaatgcgt aaacccttgc ggtctgattc atcgcgcacc
    22861 tcggcgatac catcgatttc cttgccgtga cgaatcttgt cgagttcata aacaagcgaa
    22921 attttaaccg ttttatacgg tatttcggtg atgacaattt gcttgagacc tttttcattg
    22981 atgatctgcg ttttagcggc gacttcgatg cgaccgcgac cggtttcata catgttttgt
    23041 aaaccttcac cttcgtagat gaggccgccg gtcggaaagt ccggaccctt gatgaagcgc
    23101 atcaaatcca aaggtgtaac attgacatga gtcaggcgcc agatgacggc ctcgacgacc
    23161 tcgcgcaagt tatggggagg gatctcagtt gctaatgcaa ctgcaatgcc ttcgctgcca
    23221 ttgacgagga ggttgggaaa ccgggccgga agaacactcg gttcaaagtc agtatcatcg
    23281 aatgtgagat ccatgtcgac cgtctttttt tcgaggtcac gcgtcaattc ttcggcgatt
    23341 tcgctaagcc gtgcctcggt gtagcgatag gcggccggaa catcgccgtc gatactgccg
    23401 ttattgcctt gaaaatcgac gaggggaacc cgtactttcc aatcttgcga catgcgggcc
    23461 agggcatcat aaatcgaagc gtcgccgtga gggtgatatt tacccatgac ggcaccgacg
    23521 gtatgggcgc attttttggt gggccgcgtc gaaatattac ccgattcata catcgcgtag
    23581 atgatgcgcc tttggacggg ttttagacca tcgcgaacat cgggaatagc gcggtcttga
    23641 atgacgtatt tggcgtaagt cgcatagcgg tcacccatca cttcttcaag cggttggttg
    23701 ctgatttttt ctttgattac ttcaacggta tcaggagctt ttttaaccat tattttatct
    23761 cctcaatgaa gtggtcaact ttgttgaagt cgacattttc ttcgacccat ttacggcgca
    23821 ccgccgcatc ttttcccatc aggacactga cgcgtctttc gacgagaagc gggtcttcga
    23881 tcttgacgcg gacgagctgt ctggtccgcg gattcatcgt cgtttcccac agttgatcgg
    23941 cgtccatttc gccaaggcct ttataacgcg aaattttata tcccgcgccg atatttttct
    24001 tggctttttc taagccgcga tcatcccagg catattcata aacaatggtc ttgttgacct
    24061 ctttgtaaac gcgatataaa ggagggacgg cgacgaatat tttgcccttg ttgacgaggg
    24121 gtctcatata gtgataaaag aatgttaaaa gcaaggtctg aatatgtgct ccatcggtat
    24181 cggcatcggt catgatgatg attttgccgt atttggattc gtcgatgtca aagttttgtc
    24241 cgacaccggc tccgatagtt gagatgatcg tcgagaactc ttcgtttttt agcattcgct
    24301 ccatggtgac attgtcggta ttaagaggct taccacgaag tggtagaatc gcctgatgga
    24361 gacgatcccg gcctttgcgg gccgtgccgc cggccgattc gccttcgacg ataaataatt
    24421 catttaaagc gtattctttg gactgacaag gtgtcagttt atcgctgata atcagatcgt
    24481 ttctagcttt tttagtgctt ctggcctcat ctttggcctt gcgggctgcc agacgagcat
    24541 cacgcgaatc gctgatcttt tttactaagt tgacggcgaa ctctttgttt tcatttaaat
    24601 agtaagttaa cttactataa ataaaattat tgacggcctg aatagcttcg ggagtgccga
    24661 gttttgattt ggtttgacct tcatactgga ggagcgtttc ggggattttt aaagaaagaa
    24721 tcgccgtcaa accctcgcgg atatcaccgc cttcaatctt gtttttccct tttaaaaggg
    24781 aatgttcggt gacgaagtca ttgacagccc gggttaaacc gatgcgaaag ccggtctcgt
    24841 gagtaccacc atcgcgagtg cggacattgt tggcatacga ataaatcgac tcattatagt
    24901 cctcgatgca atattgcatc gcgcattcga tgatgatacc gttggcttca tcttcaaatg
    24961 agacgatatt gcccagcggc ttcttgttcg tatttaatgt cgcgacatac tctttaaggc
    25021 cgtcgacata gtaaaattcg tgggataaat cggttctttc atccttaagg atgaagcgaa
    25081 ctttctttaa gagaaaagca ctctcttgca agtggttata aatcgtatcc catttaaaat
    25141 caatcgaggt gaaaatcgtc gcatcaggct taaatctaac aagcgttcca tgtttgttgg
    25201 ttttgccgat aatttcaagc ggagtcacca attttccgcc attttcaaaa cggaggtgat
    25261 aaatcgaact atcgcgatag acggtgacgt ccaaaaactc gcttaaggca ttcgtgaccg
    25321 aggcgccgac accgtggaga ccggcgctgc ttttgtagac ggcatcggag aattttcccc
    25381 cagagtgcag tgttgaaaag atgagctgca ccgccggcat attcgactgt gtgtggaggt
    25441 cgacagggat tccgcgacct tcgtcttcga ccgacagcga gccatctttg tgaatgatga
    25501 ccgtaatttt actgccaaaa ccggagagag cctcatcgac gccattatcg attacttccc
    25561 agacgaggtg atgcaaacca gaagagttcg tcgaaccaat atacatcgcc ggtcttttgc
    25621 gaacgccaga aaggccttca agaatatcaa tatcagcagc cgtatatgtg tttttaatcg
    25681 ttttttcggt catcttgtcc tcgcgtgttc taaatcaatt cgtaattata acaaataagt
    25741 tttgaaaagt aatgatactt ttatataata aataagcatt taacgggtga ggacttatgg
    25801 atattattta taatatagca attggattag gatcattaat aattggttat ttaatcggat
    25861 cttttccgac ggcaatcgtc atcgggaaag tctttttcaa gcaagatccc cgcgatttcg
    25921 gttccaaaaa cgccggcggc acgaacgccg ggagactatg gggcaaaaaa gccggactcg
    25981 ccgttatgat cgtcgacata accaaagccg ttctctcaat ttgggccgcc tatctgctca
    26041 tcaacctcac cggcctagcg aatgccgtcg ctttatggcg cggaggcatg gcttactact
    26101 acttggcatc ccttggtgcg acgcttggtc actgctggcc gctgtacgcc aacttccgcg
    26161 gcgggaaagc cgtctcggct cttgctggaa tggtcgtggc gacgagctgg tttctatcca
    26221 ttttcggaat ggttttcttc gtcattctta aaaataaaaa gtttgtttca ctctcgtcaa
    26281 tcatcgcctc ttcaaccgtt gttctagcgg ctctggcgat gtttcttacg gactattttg
    26341 tgcccggtag tttcaacttc ggtatgtggg gaaacgggaa attattcttg gtgggctggg
    26401 actatttcgc gaccatcttg atcatcacta tcatcctcat ttttagacat cacgaaaata
    26461 tcaaaagact cgctcgaaat gaagaaaata aagtgcgatg gatcaaataa aaatagttta
    26521 taattagtag ttatttagtt aaaaatgcaa gtgtgaataa tttccacaca ataagcgtcg
    26581 ttataaaaat aattcgatta aatcttattt tttgattgac aaacagacaa tagaactgac
    26641 ctccgcgtct tttgaaaaca aaatcgcacc acagcagtaa atactgatca tcaccgtctt
    26701 cgcaaacata aaaaaagacg cccgagaagg tgtctttttt tgctgtcaga agcgggtgct
    26761 cactgactgt ttttcatgtt gttcatgacc gccctaattt gggcttcgct cggcttgcga
    26821 cccattgaaa ggaacatcgc gcgaatcatg ttttcgttga tgggtgggtt cttggcgagc
    26881 tcgcgtttaa agagccatct cgaaccgaag aaaccagcga cgagtccgcc gatgagggcg
    26941 aggacgatga gtaaaatcca tgaccatact tccatttgtt tttctcctta tgcgcggccg
    27001 tcgtattggc cgttatcagt tctgactaaa atcgtttcac cgctttcgac gaagagcggc
    27061 actcgcagtt tcaagcctgt ttccaaaatg gcatccttca tcgccttgtt gacggtgtcg
    27121 ccgcggaccg ccggttcgca ttcgacaatt tgtaatgcga ctttggcagg taaggaaaga
    27181 ccgagaatct catcttcata agccaaaatc tcgatttcgg cattgctgac gagaaaattt
    27241 ctttcccatt ccatgttggc tttcggcaat tcaatctgct cataagtgtt ttgatccatg
    27301 aaaacgagca tgtcgcccga atcatagaga taagtcatct ttttcttgtc gaggcgtacg
    27361 ggttcgacca catagccgct gttgcgcgag atttcaagaa tggctccggt gcgaaggttt
    27421 ttgactttga ctttcgccac catctttctc atcgctgtct tattaagcaa aatatcaaga
    27481 accgagtata aaacgccttc gtctaggaag tggtttcccg gtctaagttc tgtaacatta
    27541 atcatttttg tatcctcctt ttgccgtttg ttattataac aaagttattt attgttgggt
    27601 atgtattctt ttcgcaagta agtattaaac ttcaattccc gctcgatcgt cgtacttttc
    27661 tcgtggccgg gatagacttt agtttccgga ggcagggact ttaatttagc taaactttga
    27721 acgatgctcc gcggcgaacc ggtcggcaaa tcgcttcttc cgatggcgct gcgaaataat
    27781 gtgtcgcccg taaaaatcat cttctcttgg ggaatgtagt agcagacgct tccccgcgtg
    27841 tgaaaagggg tatgcacaat gcgaatatct ttttttaaaa gatagagtgc atcattatcg
    27901 ctgacaccaa gcgccttaat gctaaggctc accggcggaa tgccgctgtc aagataagag
    27961 caattgaggc gtgaatcacc aagaaaatcg acctccgact catgcatgta aacaggcgca
    28021 tcgggaaagg tttctaaaag atagggtaga ccgcgaatat gatcgaaatg accgtgagtg
    28081 agcaagacgg cctctagttt taggccgtgg cggacaagaa aatcgcgaat ggcaggtgtg
    28141 tcgcctaaac ccggatcgat gaccacggct tgcccgtctt cgctcagaac ataggtattg
    28201 ctgacgaaat cggactcgtt gagaagagtg acgagcatct ttaaaaaaga aggcctaagc
    28261 cttacttttt ctccttatga atcgtttgtt tattgcatct tgagcagaac ttctttattt
    28321 cacggcgctc gggatgattc tttttgtttt tgctcccgat atagttttct tcaccgcaaa
    28381 cggtacactt caggataata ttgtcgcgca tttttcgtca cctcttttta aattgctcat
    28441 aaatagtagc acatataaat aaagatatct acact
//
LOCUS       DAOSSL010000008        28382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1838_length_28382_cov_18.104353, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000008 DAOSSL010000000
VERSION     DAOSSL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 28382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28382
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_28382_cov_18.104353"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..507
                     /locus_tag="PLH17_01665"
     CDS             85..507
                     /locus_tag="PLH17_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOR20523.1"
                     /translation="MEIARYEKRIIAYLIDLILAFAPPLAAAIVVYFRLPPNFDLPLY
                     FYFLGVEIASYLLFIIFSFFFIIISKGFTFGSLIMGIKIMHPNRIPLTARDAFLRGVT
                     IGVLPMVLANAVYMLAIHTERTIFDRITETVVVDYRHR"
     gene            646..1704
                     /gene="hrcA"
                     /locus_tag="PLH17_01670"
     CDS             646..1704
                     /gene="hrcA"
                     /locus_tag="PLH17_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOR20524.1"
                     /translation="MNRRDKILKLIVDHFIKTAQPVGSQTLLDEYHLEYSSATIRNEM
                     NALEVDGFIEKPHTSSGRVPSSKGYRYYVDNLRESSLDEEIKHRIQTVLDHKVQSAEE
                     VIKESCEILSHMTNLVSVVLGPNAQEEKLVNLQIIPIGERSATAVFVTDTGYVENKTF
                     NVGEKIKLEDVQSCVKLLNDRLAGTPISELVLKMQAIKPLISDLIVGHDILYQAMLET
                     FLRFASDRLSLYGRDRLLDQPEYSKDINKLKKMMKMLSSPDMLRDIAEQGDSEMTIRI
                     GEDDNDLEDVAVVSAKLKVPGQKEGSIAIVGPRRMDYAKVINYLEYVVASIEKHFEEQ
                     DIKEPAPAKEEDDKWKKN"
     gene            1689..2312
                     /locus_tag="PLH17_01675"
     CDS             1689..2312
                     /locus_tag="PLH17_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOR20525.1"
                     /translation="MEKELKPEEKKDVKAEEEGKDKEPRSFRHKLEVKEAEIEKWKAD
                     ANHWKNEYYRGYADTQNLRKALERELGEGLKYRAEGFVAELLPILDGFYFALAMEPAN
                     PEIKNYLVGFQYIYQNLLAVLEKEGVKEIAPKEGDPFDVRTMHAMEAEEREGQPNLVV
                     RVLNKGYKLKDRLIRPANVVVSKAKAAEVKKEEDGATNKEDGVKKDA"
     gene            2331..4169
                     /gene="dnaK"
                     /locus_tag="PLH17_01680"
     CDS             2331..4169
                     /gene="dnaK"
                     /locus_tag="PLH17_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOR20526.1"
                     /translation="MAKEIILGIDLGTTNSVVSYLQADGKVKVIPNPEGTNTTPSAVA
                     FKANGEEIVGNAAKRQAVTNPDTVLSIKRKMGTSEKINVKCIKKDFTPQEISAKVLAY
                     MKKYAEDNVGQAIKKAVITVPAYFNDAQRQATKDAGVIAGLEVVRIINEPTAAALAYG
                     LETKKAGKILVYDLGGGTFDVSILDIGDGTFEVVSTSGDNRLGGDDWDKVLADWIKAQ
                     IKITHNVDLTDKMAEQRFVEAAEKAKIELSASLETTISLPFIGMSSAGPINFETKLSR
                     AKFQDLTKHLLARTEAPLKRALADAKLTANDIDEVLLIGGSIRMPAVQELVKKITGKN
                     PNLSINPDEAVSVGAAIQGGIVAGDVKDVVLLDVTPLTLGIETLGEVMTPLITRNTTI
                     PTTKSQIFSTAADNQPAVDIMVYQGERPMAKDNKLLGHFQLTGIKPARRGEPRIEVTF
                     SIDVNGIVKVTAKDLDSQKEQQITISGTSGLSKADIDRMIREADENREADEKRKEEVE
                     VKNKAEQYIASIDQALAEKGDSLEAAQKEQTTKLRDELKAALDANDIDTLRNRLGELE
                     QAAAMMANAGAKAPHAEAGPEPASDGKNSEQAGDDVIDADFTEKKN"
     gene            4228..5364
                     /gene="dnaJ"
                     /locus_tag="PLH17_01685"
     CDS             4228..5364
                     /gene="dnaJ"
                     /locus_tag="PLH17_01685"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOR20527.1"
                     /translation="MATKRDYYEVLGLKKGASKDEIKSAYRRLAKKYHPDINKEAGAE
                     AKFKEVQEAYDILYDDNKRATYDQFGHAAFEQGTSGGGNPFSGAGFSGAGFGDINLGD
                     IFNSFMGGGTRQRVDPNSPRRGDDNLMRIRIDFMDAINGRKYAIPVTYDETCSTCGGS
                     GAQSAKDIHTCSNCHGKGYVRTQQRTLFGIMEGQTTCQVCGGSGKIIDNKCSTCGGKG
                     YNRVKRDIDVSVPPGINSGQQIRIQGRGGRGHNGGPNGDLFIEINVNPHPHFKRDGNN
                     IHITIPVSFVDAALGTSIEVPTVYGDVEVKIPAGTQPDNILKLKGKGIKDMRNGTPGD
                     EFIHLDIKTPKNLSKKQKDLLENYRSLEGSGENVFQKFRKSFTK"
     gene            5462..5920
                     /locus_tag="PLH17_01690"
     CDS             5462..5920
                     /locus_tag="PLH17_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOR20528.1"
                     /translation="MNKFMRMAINEARKGIHSGHGGPFGSVIVKDGVVVAKGHNQVIK
                     NQDPTCHGEMMAIHKACKKLGTFDLSGCELYTTAEPCPMCLGAILWANISRVYFGCNI
                     VDTEEIGFRDKVFYEINESGKKDEIVIELDRKQCLKLFEEYKNIEDKHHY"
     gene            5922..7352
                     /locus_tag="PLH17_01695"
     CDS             5922..7352
                     /locus_tag="PLH17_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOR20529.1"
                     /translation="MKRFFASFSVMFANSKTEHLYSVNGRVPLLKAIPFGLQHVLSMF
                     VANITPVIIVFGLLGDPELTAQAVRGAIFMAGIGTIIQVTLGSRLPVVVGTSFTFVSA
                     LVAIGNPGSIFGALIVGGLIILILGIFAKYWRRWIRPVVPAAVVLAIGLSLLSVGSAQ
                     FFGGSNYLSQVLSGAASNELLWQYALVAFITLSTAIAWNIFIKGVWKNLAILVAMAVG
                     YLVALLFPGMVNLSLIGSGGIIALPSFIDFSSLRFELVPILIVSISYIASSVECIGDT
                     TTLARMGLGRDPSDREIAGAITADAANSIVGALFGSLPLTTFSQNVGIVAQTKVVNRF
                     TIFIGALFLVIASLFPPIANLLITIPEPVLGGCMLILFGSIAVIGMQMVAEIGFDSKT
                     ILVLAISLTLGFGITLVGDFYRVLDTFGFEYLSVIFASPILNMFVISMILSYVIPEKK
                     KKAVVTAPENQAENASNGEEKQITGK"
     gene            7481..9592
                     /locus_tag="PLH17_01700"
     CDS             7481..9592
                     /locus_tag="PLH17_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR20530.1"
                     /translation="MEGPIDYGKDPDVLKKFGRLITEEARDGKIDPVIGRDEEIRKII
                     QILSRKTKNNVIMLGEPGVGKTAILEGLAQRIIKADIPASLKDKDIYELDMGALVAGA
                     KYRGEFEERLKAVLNKIKESEGKIILFIDEIHLIVGAGRSDGALDASNMLKPMLARGE
                     IDCIGATTLNEYRQYIEKDRALERRFQTVLVEEPSPEDSISILRGLKERFETHHGVKI
                     TDQAIVAAVTLSTRYITNRFLPDKAIDLIDEACASIRVEIESMPAELDAITRKIRQLE
                     IEKVALSKEKDSISLERFKKLNEELESLNAEERNLAKRWHDEKDAIQSIKVIKSQLER
                     AKFELDTAFNRGDYKLASELQYKRIPDLEKRLAELEKIDKGDHLLSEVVTEEEVAKVV
                     AKNTHIPVSRLMQGDRDKVLGLKDTLKKRVIGQDQAIGLVSDAIVRQRAGIANPNRPI
                     GSFLFLGPTGVGKTEVARSLAEALFDSETHIIRIDMSEYMEKFSVSRLIGAPPGYVGY
                     EEGGQLTEKVRRSPYSIILFDEIEKAHPEVFNLLLQVLDDGRLTDSQGRTVNFKNTVI
                     IMTSNLGSEYFIAGKPEMVDSLVKSTFRPEFLNRIDELVYFLPLAKDTQIKIVDKLLG
                     ELSTNLKSQYVELTFSEALKRHVLDSSFSLEYGARPLKRFIQKEIETAIARKLIDGSI
                     EPHRAYLLDVDSGGNLLVKNI"
     gene            9693..10136
                     /locus_tag="PLH17_01705"
     CDS             9693..10136
                     /locus_tag="PLH17_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOR20531.1"
                     /translation="MPNCRGCHRKIERLDKDICPFCGTPNPIPGSQSLTVDITGVISG
                     AGVPKDELPRACSRKRAFTLCALFGFLGIHAFYVKKPKQALFFILFSLCLIGGVGSLL
                     FFFVLPGSIWAFLIPVFVQIIFQMIFAFHYLTSEDLKDGVGELMH"
     gene            10136..10852
                     /locus_tag="PLH17_01710"
     CDS             10136..10852
                     /locus_tag="PLH17_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOR20532.1"
                     /translation="MQRYFGQVFEGRAILGEGDAFHLAKVMRCQIGTEIEVVADGHVY
                     LVRVETIKPLFLKVIGTINETRELPNDVVLVMAPLKGDKINLVLQKATELGVNEIVLL
                     LTSRTIVRFNHKDIIAKLTRFRAILKEAAEQSQRTHIPTISYVDSISKLNSLTAKHKF
                     VAYEQQSGPTGGFNKLIKQIKKRERVAVVIGPEGGFAGEEVDELKNLGFIPVSLGQRI
                     LRAETASIYALSVIANFLEN"
     gene            10854..11654
                     /locus_tag="PLH17_01715"
     CDS             10854..11654
                     /locus_tag="PLH17_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20533.1"
                     /translation="MNIFEDLQLNQKFQGFLESEENLFYLKNRIILERFPMYKHLAQA
                     TSDFDIIRANIIIRSLQNKKAIEAAVRFAVSNNDVIKREKDMAFFRTFIREKIIDLPF
                     ATDQKNRLYVPLFNRVINSIYSEEFEKLLLSPYDKLVDEFETLVVDPFEIYNFSLFDS
                     LFTQFVKVYSDDDIMAVFHYDFQAIYFIDKQGRLDNKLALFDRGLRHPDFHHLLERVR
                     PVIAAYIAHDKEAMLTALAEKKLISEKLISRLRSKDYQFRMSLIRKIK"
     gene            11654..12946
                     /gene="mtaB"
                     /locus_tag="PLH17_01720"
     CDS             11654..12946
                     /gene="mtaB"
                     /locus_tag="PLH17_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOR20534.1"
                     /translation="MNFLLFTLGCKVNAYESDAVRELFLSQGWREARSGDEVELVVIN
                     TCTVTGTAAKKSRQHIRKFRLAYPRALLVVMGCYAQEEGTSIIAECGADLILGTSHRK
                     EVVQLIKEVQKQPLRRAVIEAEPRQFDYEEIATLMQPMTSRAFVKIQDGCDNFCSYCI
                     IPYVRGKSRSRDPQEIKKEIRMLVDNGYQEIVITGIHTGGYGRDLANYSFSRLVREIC
                     EEINGLYRLRISSIEATEIDDDFIALLASSSVIADHLHIPLQSGSETVLKRMNRRYRI
                     SDFIKTVNQVRQARPGIAITTDVIVGFPGETDAEFDETVALIRAISFSELHVFPFSKR
                     ENTAAAKMSDQVESEVKKKRVNTLLKISEELHCDYEQNYYGKTLDVLFEDFNEADQTY
                     RGHTSNYLEVHLKCQENLSGKVSSITYRGPKNSELKKA"
     gene            13019..13192
                     /gene="rpmF"
                     /locus_tag="PLH17_01725"
                     /pseudo
     CDS             13019..13192
                     /gene="rpmF"
                     /locus_tag="PLH17_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            13296..13937
                     /gene="deoC"
                     /locus_tag="PLH17_01730"
     CDS             13296..13937
                     /gene="deoC"
                     /locus_tag="PLH17_01730"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOR20535.1"
                     /translation="MEYNKLIDHTLLKPDASLAAVAKLCDEASEHDFMSVCVNPCFVP
                     VVKKLLEGSLVKVCTVIGFPLGATLPDVKVFETKRAVELGADEIDMVINVSMAKAHDY
                     EFIANEINMIKVACQGRLLKVILETCLLTNEEIVACCRAAVKAGADYVKTSTGFSSGG
                     ATVEHVKLMRDTVGPQIGVKASGGVRTKEDLEKMVAAGASRIGTSSGVKIVAK"
     gene            13977..14150
                     /gene="rpsU"
                     /locus_tag="PLH17_01735"
     CDS             13977..14150
                     /gene="rpsU"
                     /locus_tag="PLH17_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003142732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOR20536.1"
                     /translation="MPKTVVRDNESLDEALRRFKRQVNKAGTLQEARKREFYLKPGLK
                     KKLKSEMARRKKY"
     gene            14230..15426
                     /locus_tag="PLH17_01740"
     CDS             14230..15426
                     /locus_tag="PLH17_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOR20537.1"
                     /translation="MRYFTYQGNDYDCGFASLKMLLCYAHKTPKYLRLAKPEEKRSRY
                     SYRDLLDIAARHGVVLTAYKLIDKGEIKKQKKWPILVSLTSENGSLHLILLRRIGKKV
                     LADDPASGAVTMTYEDFLRRWDGSFLEVTKVIEPPRFTKEKPIVAGSKRFLLLILQSF
                     SALFALMGFAFVSSENYFVYPLLFFVLFIACELLFRRFQLSVLAEFDERYMHRTFDDD
                     KLLMHTKFSKFHDFKKDYFLFPQIIVSALIISCFALLVLALNDMRHLVFLATLFAFAF
                     FDFLLCKVIDDKHEKLLRKLEVDSFRDILSKSDAISNYQKMSSLAQLIAKSAIARKYV
                     GLFLVGAAALTYAAISGAATLNFFLFHFFIYALFYEHVGKLLKSLLHIGEIRREIAAF
                     KDQFVR"
     gene            15473..15928
                     /gene="ybeY"
                     /locus_tag="PLH17_01745"
     CDS             15473..15928
                     /gene="ybeY"
                     /locus_tag="PLH17_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOR20538.1"
                     /translation="MEITFTNTGPSGLNDYETIYRDLAVSAIAFLKITAEYIIEVNLV
                     SDEEIQAINKAYRGKDAPTDVISFAFLDSIETEVAIKGDVPRLLGEIMISYETAERQA
                     KKYGHSVQREMKFLFAHGILHLLGYNHETIDQEKVMFALQDQILDQEKK"
     gene            15928..16326
                     /gene="cdd"
                     /locus_tag="PLH17_01750"
     CDS             15928..16326
                     /gene="cdd"
                     /locus_tag="PLH17_01750"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOR20539.1"
                     /translation="MNREQLIELAVEARSLSYSPYSHFAVGAALLCKDGSVYVGSNVE
                     NASYPLSMCAERNALYSAFMDGKSKEDFVAMAVVGDTDSPITPCGACRQVISELFPMN
                     APIYMANLRGDVKETTIAELLPFAFDGSDL"
     gene            16327..17004
                     /gene="era"
                     /locus_tag="PLH17_01755"
     CDS             16327..17004
                     /gene="era"
                     /locus_tag="PLH17_01755"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOR20540.1"
                     /translation="MKSGFVAIIGRPNVGKSTILNALINQKVSIVTNKAQTTRDNIKG
                     IYEDDEAQIVFVDTPGIHKPHHELGRIMNQSALASTSGVEAVILVVDVAKPFGDGDAF
                     LVGELKVDCPLIIVLNKIDLAKIDQVQSLKARYQELYPKAHIIEMSAIRLFNVDDLLK
                     IIKSHLEEGPRYYPLGSVSDRNESFFIKELIREQVLMQLREEVPHQIAVAVEKFQLKS
                     KASSSKL"
     gene            17198..17917
                     /gene="recO"
                     /locus_tag="PLH17_01760"
     CDS             17198..17917
                     /gene="recO"
                     /locus_tag="PLH17_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOR20541.1"
                     /translation="MEIIGIVTRLTPFKEKDAMINVVAENKAYSFLARGVLGIASKNM
                     ASVQPYTKSRFVLTKGKEGLSLRTGTVIDSYAKAKEGLAGLTALSFIGELTNKAIGGE
                     EAAKIFPALGMALANIDAGFHPLTIVLLYFAAVLKAIGFGLEVTKCVISGQRSDISAI
                     SYLDGGFVATPYFDALRHQKRNERQLKIIRQIFLAEATDFDRVAFTSGECLEIIKDLS
                     VYIQDGAGINLKSIFLLEKSS"
     gene            18005..19372
                     /locus_tag="PLH17_01765"
     CDS             18005..19372
                     /locus_tag="PLH17_01765"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOR20542.1"
                     /translation="MKYDFDTIVNHLITSGFVYQGSEIYGGLSNTWDYGPLGSELKNN
                     LKKAWWKKFVQESPTNVGIDASILMNPRVWEATGHVSAFNDPLIDCKSCRSRHRADDL
                     ISKAHPEINVTGLTNEEMNVLIKDKKIACPVCGKSDFTDIRQFNMMFKTHIGVVEDSK
                     SVVYLRPETAQGIFVNFRNIQRTTRRKLPLGICNVGKSFRNEITPGNFIFRTREFEQA
                     ELEFFCKPGTDLEWFEYWKNYSLKFLRSLNINQDKLRYRDHDSKELAFYSKATCDIEY
                     EFPFGWGELLGIADRTDYDLKRHMEYSGESLEYLDSDSGEKYVPYCIEPSFGVDRLML
                     TVMCDSYVEEQLEKDQRVVMKLHPALAPYKVAVLPLSKQLNEKAKEVVNILNKYFAVT
                     YDETGSIGKRYRRQDAIGTPYAVTVDFETADDQSVTIRDRDTMNQIRLPIKELVGYFG
                     VKLFY"
     gene            19420..21219
                     /gene="dnaG"
                     /locus_tag="PLH17_01770"
     CDS             19420..21219
                     /gene="dnaG"
                     /locus_tag="PLH17_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOR20543.1"
                     /translation="MIYESSLVDDILKQADIVNVISSYINVIQKGRNYVALCPFHDDK
                     NPSMMISKEKQIFKCFVCGAGGNAIGFVEKYEKIPFSSAIRKTAEIIGFQDIRLNKPI
                     SHVQVDETIAPLHECIEDLTTYYQFGLSTEEGSKARAYLEGRHIDLDQQERFRLGYAI
                     KDGKGTVSYLQQKGFSLKTIEGIGIALAKAEGTSDFNAGRLIFPIHDGDGKVIGYSAR
                     KLFDETEGPKYVNSPETKLFHKSSVFYNFHNAKLTAKHDGYLYVLEGFMDVFALDKIG
                     IKSAIAIMGTALTREQASMLRRLNVEIRLCLDGDAAGQMAMMRIMGVLDAADVKYRLV
                     SEPGNEKDPDEILQEDGPEALRKYLNMLVDPFEFALNYYQHTNPLGSLEDRQKVVNHF
                     LPMLVQLSKGIDLDNRIFRLSKVTRFDPQAIAKFVDEARSRVSTERPMITELPEMEFK
                     PFRKDIRRLQNAERQLLYSMFKDKNAVDFYEKNVQYFYIEVYRHIALHIIDYLSKHEE
                     IDVSLLINEIAMKEAPNKDEIIDEITSLNAKNMYPDPTEQSLNEMRDVIKEETQNLYE
                     KEALKKSLLGKTPREQARIIDDYNRRKMKKIGK"
     gene            21236..22636
                     /gene="rpoD"
                     /locus_tag="PLH17_01775"
     CDS             21236..22636
                     /gene="rpoD"
                     /locus_tag="PLH17_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOR20544.1"
                     /translation="MAKKKIDEEAVAKDVEAAQPARSKVAQTPKIKKGGEVKKDAKVK
                     KEKVKAKTSKKIDKVSDEEEEPVIIVDEEEEIESLETIKKRLVKTFKERGFIDMSEVL
                     DETSHLDLSDADVEDLIVFLKSKGVDVISDIDAEAEIDDIPEEEIAKGDSLVDEDLID
                     EEEVVDEEVEALAEIDDLSKLATGEVKVNDSVKMYLKEIGRVDLLRPEEEPILAKRIL
                     EGDMAAKNELINANLRLVINIAKHYSRGKAMDFLDLIQEGNLGLMKAVDKFDYTKGFK
                     FSTYATWWIRQSIARAIADQARTIRIPVHMVETINKMKRVQRQLVQRFGREPTAEEIS
                     EELEGFLSPERIREIQRIALDPVSLESPIGEEEDSHLGDFIEDKEAETPVEFTTKSLL
                     KDELYEVMKDLTDREERVLRLRYGLDDNRPRTLEEVGREFGVTRERIRQIEAKAIRKL
                     RHPTRAKRLGDFRDSR"
     gene            22633..23292
                     /locus_tag="PLH17_01780"
     CDS             22633..23292
                     /locus_tag="PLH17_01780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOR20545.1"
                     /translation="MIRLSKRLSAIANFVDKCAVVADVGSDHGLLLIYLASLGKIAKG
                     YGIENKPGPYQILKHNLESHSEAELKPLLQDGISNLEKDVDTIVLAGLGGDTIVRILE
                     NGVANLKNVKTIITDSHTSIGEVRRHIVALGFIIKDEVLVLEKDKYYEICKFVKSDER
                     PAYSDFEYRRGPIIIKSPAFRHYAKVLIKKMDAILEKDLPEAMKELVRQEREVLLPYE
                     N"
     gene            23282..24064
                     /locus_tag="PLH17_01785"
     CDS             23282..24064
                     /locus_tag="PLH17_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOR20546.1"
                     /translation="MKTKALVRKLATRFPKRIAEPGDRVGLMTGKFKEKTKRIVVCLD
                     FDEEVMRHIEALDGGPVDLILTHHPFLYRSRYQVFKKDEIKRRLTEKVDALGIPVYSM
                     HTNFDSGKDGMNDALASALKLSGIRQLESMPMARGGRLAQKMPIKQFAEVAARLLNVP
                     YGLLINAGKEMVETVAIIGGGGSSYYQNALNEGYDIFISGDAPHHVRRDIVLREYNYL
                     DLPHEIERIFISQMGKILKEIDADLEVIAINHEETPEVIIVS"
     gene            complement(24051..24920)
                     /gene="ispH"
                     /locus_tag="PLH17_01790"
     CDS             complement(24051..24920)
                     /gene="ispH"
                     /locus_tag="PLH17_01790"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOR20547.1"
                     /translation="MNIFISRPHGYCSGVIRAINLVKKARQKHVGVPVFVLGSIVHNE
                     DVIQELFSLGITTVRDLKKSPAQLLKELPDQAVVVFTAHGHEVSLDEIARQKKMVVYD
                     ATCPMVTTNHRIIQNELNNDHQVIYIGKKNHPEANAAKSLGDNVFLIENNEDVNLLEL
                     VDKSPLVINQTTLSQTELAALHTSIISKFSKARIADEICNATRLRQQAVLALPKETEL
                     VYVIGGVNSSNTASLADMARKTLPQTRVIRILNADEINKKDLDGLNYVAVVSGASTPL
                     ETTNRVVEELKRR"
     gene            24977..26335
                     /locus_tag="PLH17_01795"
     CDS             24977..26335
                     /locus_tag="PLH17_01795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR20548.1"
                     /translation="MSFKSFNLSDLMIKSLARQGYLSPSSIQTRVIPKTLKGGNIVAQ
                     SETGSGKTHAFIIPIIENLQLDSDRLQALIITPTRELAQQIYAFLEAFKGDYPKLHAR
                     LFTAGKETERTFRQGMRVPHVIIGTPTRIKTMLEAKSLIDLGSVKTLVLDEADMLMEM
                     GYFADIDIIYRLLKAPPQMMVFSATIEFNLRKNLEKYVGADFVIEMDDVKTAKTVGHH
                     AIDVKHGDRLHAIEIFIKTLNPYLLIIFASRKEQVATISSYLKSAGHDNIMLHGDMSA
                     RERKSAYKLIGANKHRLIVASDLAARGLDIKDVSEVLNFDLPKDLTYYFHRAGRTGRF
                     GASGQCYSFYNSDTVTQLEKLIESGVSFSFHELRDDTIISTDKIARRKTRKRKVDPDL
                     ERQIKLAAAKVKTDVVKPGYKKKVRDAVAKVKRKHKREIIRKDIRRQRVARYRKEGSK
                     NE"
     gene            26337..27209
                     /locus_tag="PLH17_01800"
     CDS             26337..27209
                     /locus_tag="PLH17_01800"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOR20549.1"
                     /translation="MFIGSHVSMSGPEYYLGSVKEALSYGANTFMFYTGAPQNSYRKP
                     VGELKIKEGRKAIADAGIDESKIIVHAPYIINIGNKTNPTVYESSKKFLIEELRRVAA
                     FGPRILVLHPGSHVGIGVEGGLVSVVEALDEVLEQDGTNVRIALETMAGKGSEVGATF
                     EEISYLLTHSRHPERLGVCLDTCHINDAGINVHEVDEVLNTFDRVIGLEKLLCLHVND
                     SKNVMGAHKDRHANIGYGEIGFETIHAYIKHPRLENVPKILETPYIGEYAPYQKEIAM
                     LREGVFEEGWIEKL"
     gene            complement(27236..28294)
                     /gene="ispG"
                     /locus_tag="PLH17_01805"
                     /gene_synonym="gcpE"
     CDS             complement(27236..28294)
                     /gene="ispG"
                     /locus_tag="PLH17_01805"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOR20550.1"
                     /translation="MLPRELTRKIQIGDRAIGGQNRVLIQSMCSIKTSRIAEVVAQIR
                     RVTLLGADLMRVSVLDEEDAAAIREIKKQISIPLVADIHFDYRLALLAIENGVDKIRI
                     NPGNIGSEDKIKAVVKACQKHRVPIRVGVNSGSLDKDIAENSVFSHAEKLVASAQKHV
                     AILEKYGFHDIVISLKGSNAIETIEAYRKAAKTFPYPLHLGITEAGPKDIGLIRSAAG
                     LAPLLIEGIGDTIRISLSDEPEEEILAAKRLLHDLDLYERYPTIISCPTCGRTQVNVV
                     PMAKRVLAYLEEHNLNLKVAVMGCVVNGPGEARHADYGMAGGNGIWVLFKKGKLLHTV
                     KEDEVFDALIATIEKDIT"
BASE COUNT         7796 a         7175 c         6677 g         6734 t
ORIGIN      
        1 gatgataaaa tcggaggagc aaattcgcct ttctgatgac ggattgatga ttttggatcg
       61 aattttgctt acaataatat agttatggaa atcgcccgtt acgaaaaacg cattatcgcc
      121 tacctgatcg accttatttt ggctttcgcg ccgcctctgg ccgccgccat cgtcgtctat
      181 ttccgtctcc cccctaattt tgatctgcct ctttatttct atttcctcgg tgttgaaatc
      241 gcctcttatc tcttatttat catcttctct ttcttcttta tcatcatctc caaaggtttc
      301 actttcggat cgctgatcat gggcattaaa atcatgcatc ccaaccgcat tcccttaacc
      361 gcccgcgatg catttctccg cggggtgacg atcggggtct tgccgatggt tctcgccaat
      421 gccgtctaca tgttggcgat tcacaccgaa cggactatct ttgatcgcat cacggaaacg
      481 gtcgtcgtcg attatcgcca tcgctaataa ctaatttcac tattaaccac acttgtgtgg
      541 ttttcttttt gctcaatttt agcacttatt acttgacagt gccaaaagac tctttataat
      601 atagttagca ctcaggctac aaaagtgcta atcaagggaa aaacaatgaa ccgccgcgat
      661 aaaatattaa aactgattgt cgatcacttc atcaagacgg ctcagcccgt cggaagtcag
      721 acgctgctcg atgaatacca tttagaatat tcgtcggcga ccatccgcaa cgagatgaac
      781 gctttagagg tcgacggttt tatcgaaaag ccccacactt cgagcggtcg cgtgccttcc
      841 agcaaaggct atcgctatta tgtcgataat ttgcgtgaaa gctcgcttga tgaggaaatc
      901 aaacatcgca ttcaaaccgt tttggatcat aaagtccagt ccgccgaaga ggtgataaag
      961 gaatcgtgcg agatcttgtc gcacatgact aatctcgtca gcgtcgtctt aggcccgaat
     1021 gcccaggaag aaaaactggt caacttacag atcatcccga ttggtgagcg ctcggcgacg
     1081 gcggtgttcg tcaccgatac cggctatgtc gaaaacaaga ccttcaatgt cggcgaaaag
     1141 attaaactcg aagatgtcca atcgtgcgtc aagcttctca atgaccgtct cgccggcacg
     1201 ccgatctctg aattagtcct caaaatgcag gccattaagc cattaatcag cgatttgatc
     1261 gtcgggcatg atattctata tcaagcgatg ctcgagacct tcctgcgctt cgcttccgac
     1321 cgcttgagtc tatacggccg cgaccgcctg ctcgatcagc ctgaatattc aaaagatatt
     1381 aataaattaa aaaagatgat gaagatgctc agctcccccg atatgctcag ggacatcgcc
     1441 gagcaaggcg acagcgagat gacgattcgc atcggcgaag acgataacga tcttgaagat
     1501 gtcgcggtcg tctcggccaa actcaaagtc cccggccaga aagaaggcag tatcgccatc
     1561 gtcggcccgc ggcgcatgga ctacgcgaag gtcatcaatt atctcgaata tgtcgtcgct
     1621 tcgattgaaa agcatttcga ggaacaagat ataaaggagc cggcaccggc aaaggaggaa
     1681 gacgataaat ggaaaaagaa ttaaaaccgg aagaaaaaaa agacgtcaaa gccgaagaag
     1741 aaggcaagga taaagagccg cggtcattcc gccacaaatt agaagttaag gaagcggaaa
     1801 tcgaaaaatg gaaggctgac gccaatcatt ggaagaacga atactaccgc ggctatgccg
     1861 atacccaaaa cctcagaaaa gcgcttgagc gcgaactcgg agaagggctc aaataccgcg
     1921 ccgaaggctt cgtcgccgag ctgcttccga ttctcgacgg cttctacttc gcgttggcga
     1981 tggaaccggc caatcccgaa atcaaaaatt acctggtcgg attccagtac atctatcaaa
     2041 atcttctcgc cgtcttagaa aaagaaggcg tcaaagaaat cgcgccgaaa gagggagacc
     2101 ccttcgatgt gcgcaccatg cacgctatgg aagccgaaga gcgggaagga cagcccaatt
     2161 tggtcgtccg cgtcctcaat aaaggctaca aattaaaaga tcgcctcatc cgtccggcga
     2221 atgtcgtcgt cagcaaagct aaggctgccg aagtcaaaaa agaagaagac ggagccacga
     2281 ataaggaaga tggcgtcaaa aaagacgctt aatcatagga ggaaaattat atggcaaaag
     2341 aaattattct cggtatcgac ttaggtacca ccaactcagt cgtcagctac ttacaagccg
     2401 acggaaaagt caaagtcatt cccaatcccg aaggcaccaa cacgacgcct tcagccgtcg
     2461 cttttaaagc taacggcgaa gaaatcgtcg gcaacgccgc taagcggcag gctgtgacga
     2521 accccgacac cgtcttgtcg atcaaacgca agatgggcac atcagaaaag attaatgtca
     2581 agtgcattaa aaaggatttt acgccgcagg aaatcagcgc caaagtcctc gcttacatga
     2641 agaaatacgc cgaagacaat gtcggacaag cgattaaaaa agcggtcatc accgtcccgg
     2701 cctactttaa cgatgctcaa cgtcaggcga ccaaagacgc cggcgtcatc gccggtcttg
     2761 aagtcgtccg catcatcaac gagccgacgg cggcagccct agcctatggt cttgaaacga
     2821 aaaaggcggg aaaaattctc gtctacgacc tgggcggcgg cacctttgac gtctcaattc
     2881 tcgatatcgg cgacggcacc tttgaagtcg tgtcgacatc cggcgacaac cgcttaggcg
     2941 gcgatgactg ggataaagtc ctggccgact ggatcaaagc gcagattaaa atcacacaca
     3001 atgtcgattt gaccgataag atggcggaac agcgtttcgt cgaagcggcc gaaaaagcca
     3061 aaatcgaatt atcagcctcg ctggagacga cgatttcatt gcctttcatc ggcatgtcga
     3121 gcgccggtcc gatcaacttt gaaaccaaac tctccagagc gaagtttcag gatttgacca
     3181 aacacctctt agcgcgcact gaagctccgt taaaaagagc tctagccgat gctaagttaa
     3241 ccgctaatga catcgacgaa gtcctcttaa tcggcggctc gatcagaatg cccgccgttc
     3301 aggaactcgt caaaaaaata accggcaaga atccgaacct ctccatcaac ccggatgagg
     3361 ccgtctcggt cggagcggcc attcaaggcg gcatcgtcgc cggtgacgtc aaagacgtcg
     3421 tcctgctcga tgtcaccccg ttgaccctcg gtatcgaaac cctcggcgaa gtgatgacgc
     3481 ccctcatcac gcgcaatacg acgattccga cgacgaaatc gcaaatcttc tcgacggccg
     3541 ccgacaacca gcccgccgtc gacatcatgg tctatcaagg cgagcgcccg atggccaaag
     3601 acaacaagct cttaggtcac ttccagctga ccggcatcaa accggcccgc cgtggcgaac
     3661 cgcgcattga agtcaccttc tcgatcgacg tcaacggcat cgtcaaagta acggccaaag
     3721 acttggattc gcaaaaagaa cagcagatca ccatctccgg cacgagcggc ctttccaaag
     3781 ccgacatcga ccgcatgatt cgcgaagccg atgaaaaccg cgaagccgat gaaaagcgca
     3841 aagaagaagt tgaagtcaaa aacaaagccg aacaatacat cgcgagcatc gatcaagccc
     3901 tcgccgaaaa aggcgacagc ctcgaagccg ctcaaaaaga gcagacgacc aagctccgcg
     3961 atgagctcaa agccgctctt gacgccaatg atatcgacac cctccgaaat cgcttaggcg
     4021 aactcgagca ggcggcggcg atgatggcta atgccggcgc caaggctccg catgccgaag
     4081 ccggtccgga accagcttcc gacggcaaaa acagcgagca ggccggtgac gatgtcatcg
     4141 atgccgactt caccgaaaag aaaaactaat catcatttgc gagcgtgggg gctaattgcc
     4201 ccttcgcttt aatagtaaag gagacttatg gccacgaaac gcgactacta tgaagtgctc
     4261 ggccttaaaa agggagcgag caaagatgag atcaagtccg cttatcgtcg cttggcgaaa
     4321 aaatatcatc cggacatcaa caaagaagct ggagccgaag ccaagtttaa agaagttcag
     4381 gaagcttacg atattcttta tgacgacaat aaaagagcga cttatgacca atttggtcat
     4441 gccgcttttg aacaagggac ttctgggggc ggcaacccct tctcgggtgc cggcttttcg
     4501 ggcgctggtt tcggcgacat aaatctcggc gacatcttca attccttcat gggcggtggc
     4561 acgcggcagc gcgtggatcc aaattccccg cgtcgcggcg atgataacct gatgcgcatc
     4621 cgcatcgatt tcatggatgc catcaacggc cgcaagtacg cgattcccgt cacctatgac
     4681 gagacgtgct cgacatgcgg tggcagcggt gctcagagtg ccaaagacat tcatacttgt
     4741 tcgaactgtc atggcaaagg atacgtccgc actcagcagc gcaccctgtt tggcatcatg
     4801 gaaggccaga cgacttgtca ggtgtgcggc ggaagcggca aaatcatcga taataaatgc
     4861 tcgacatgcg gcggcaaagg ttacaatcgc gtaaagcgcg atatcgatgt aagtgtcccg
     4921 cccggcatca actccgggca gcagattcgc attcaggggc gcggcggtcg tgggcacaat
     4981 ggtggcccca acggcgattt attcatcgaa ataaacgtca atccccatcc gcatttcaaa
     5041 cgcgatggca acaacatcca tatcacgatt cccgtcagct ttgtcgatgc ggccttaggg
     5101 acgagcatcg aagtgccgac cgtgtacggc gatgtcgaag ttaaaattcc cgccggcacg
     5161 cagcccgata acatcttaaa attaaaaggc aaaggcatca aagatatgcg caatgggaca
     5221 ccgggtgatg aattcattca cctcgacatc aaaacgccta aaaacctcag taaaaagcaa
     5281 aaggatttac tggaaaatta ccgctccctt gaaggaagcg gcgaaaacgt ctttcagaag
     5341 tttcgcaaga gtttcaccaa gtaaagccat tcagcagaag caagaggctc gtcagagtct
     5401 cttttttcat agaaaataac attcattatg ctaatatatt gattgattgt gaggaaaaca
     5461 gatgaacaaa tttatgagga tggccatcaa cgaagcccgc aaaggcattc atagcggaca
     5521 cggtggccca ttcggcagcg tcatcgttaa agatggtgtc gtcgtcgcca aaggccacaa
     5581 ccaagtgatt aagaatcaag atccgacttg ccacggtgag atgatggcaa ttcataaggc
     5641 gtgcaagaaa ctcggcacct ttgacttgag cggctgcgag ctatatacga cggccgaacc
     5701 gtgcccgatg tgcctcggcg ccattttgtg ggctaatatt tccagagtat acttcggctg
     5761 caacatcgtc gataccgaag agattggctt tcgcgacaaa gtgttttatg agatcaatga
     5821 atccggcaaa aaagacgaga tcgtcatcga gctcgatcgc aaacaatgtc tgaaattatt
     5881 cgaagaatat aaaaatatcg aagacaaaca tcactactaa tatgaagaga ttctttgctt
     5941 cgttttcggt gatgtttgcg aattccaaaa ccgaacatct ttacagtgtc aacggccgcg
     6001 tgcccttgct aaaagcgata ccttttggct tgcagcacgt tctttcgatg ttcgtcgcca
     6061 acatcacgcc cgtcatcatc gtcttcggcc ttttaggcga tcccgaactg accgcccaag
     6121 ccgtccgcgg cgccattttc atggccggca tcggcacgat tattcaagtc acgctcggct
     6181 cgaggcttcc ggttgtcgtc ggcacttcat tcacctttgt cagcgccctg gtcgcaatcg
     6241 gaaatcccgg atcgatcttc ggcgccttga tcgtcggcgg actcatcatt ctcatccttg
     6301 gcatatttgc caaatattgg cgaagatgga tacgaccggt cgttccggcc gccgtcgttt
     6361 tggccatcgg cctctcgctt ttatcggttg gtagcgctca attcttcggc ggatcaaact
     6421 atctttcgca agttcttagc ggcgcggcca gcaacgagct tctgtggcaa tatgccctcg
     6481 tcgccttcat cactttatcg acagccatcg cctggaatat cttcatcaaa ggcgtctgga
     6541 aaaacctcgc cattctcgtg gcgatggcgg tcggctatct cgtcgccctt ctatttcccg
     6601 gcatggtcaa tctgagtttg atcggcagcg gcggcatcat cgcccttccg tcattcatcg
     6661 atttttcctc acttcgcttt gagctggtgc cgattctcat cgtcagcatt tcttacatcg
     6721 cctcatcggt cgagtgcatc ggcgatacga cgaccttggc ccgcatgggt ctcggccgcg
     6781 atccgagcga ccgcgaaatt gccggagcga ttacggcgga tgccgccaac agcatcgtcg
     6841 gagctttgtt tggttcattg ccgctgacga ctttttcgca aaatgtcggc atcgtcgccc
     6901 agacgaaggt cgtcaatcgg tttacgattt ttatcggcgc tttgttttta gtcatcgcca
     6961 gcctctttcc accgatcgcc aacttattga tcacgattcc cgagcccgtc ctcgggggct
     7021 gcatgctcat cttattcggt tcgatcgccg tcatcggcat gcagatggtc gccgaaatcg
     7081 gttttgattc gaaaactatc ctcgtcttag cgatctccct cacccttggc ttcggcataa
     7141 ctctcgtcgg tgatttttac cgcgttctcg acacctttgg ttttgaatat ttgtcggtca
     7201 tttttgccag tccgattctc aatatgttcg tcatctcgat gatccttagc tatgtcattc
     7261 ccgagaagaa gaaaaaagcc gtggtcacag caccggagaa tcaagctgaa aatgcatcca
     7321 acggcgaaga aaaacaaata accggaaagt aaaaattcct tctcttcaaa gcagtcttat
     7381 ccaacaggat tgctttttct tttagcactc taacttgaca agtgctaata atcttttata
     7441 atataattgt taataatata ttattaaagg agttgatatt atggaaggac caattgatta
     7501 cggcaaagat cccgacgtgc taaaaaagtt cgggcgtcta attacggagg aggcccgcga
     7561 cggcaagatc gaccccgtca tcggccgcga cgaggagatt cgcaaaatca tacaaatcct
     7621 gtcgcgcaaa acgaaaaaca acgtcatcat gctcggcgaa cccggcgtcg gcaagacggc
     7681 tattttagaa ggtcttgctc agcgcattat caaagccgac attcccgcct ccttaaaaga
     7741 taaagatatt tacgagttgg acatgggggc tttggtggcc ggggctaaat accgcggcga
     7801 atttgaagag cgcctgaaag ccgtcttaaa taaaattaaa gagtcggaag gcaaaataat
     7861 ccttttcatc gatgaaattc atctcattgt cggcgccgga agaagcgatg gggccctcga
     7921 tgcttccaat atgctaaagc ccatgctggc ccgcggtgaa atcgactgta tcggcgccac
     7981 gacgcttaac gaataccgcc agtacatcga aaaagatcgg gctttggagc gccgctttca
     8041 aaccgttctc gtcgaagagc cttctccgga agacagcatc tcgattctac gtggtcttaa
     8101 ggaacgcttt gaaacacatc atggcgtcaa gattaccgac caagcgattg tcgccgccgt
     8161 cactttaagc acgcgctaca tcaccaatcg ctttctgccc gataaagcga tcgacttaat
     8221 cgatgaagcg tgtgcctcaa tccgcgtcga aatcgaaagc atgcccgccg aactcgatgc
     8281 tatcacgcga aagattcgtc agctcgaaat cgaaaaagtc gccctttcta aagaaaaaga
     8341 cagcatcagc ctcgagcgat tcaaaaagct caatgaagaa ttagaaagcc tcaatgccga
     8401 ggaacgaaat ctggccaagc ggtggcacga tgaaaaagat gcaatccagt cgataaaagt
     8461 catcaaaagt caactcgaaa gagccaagtt tgaactcgat acggccttta atcgcggcga
     8521 ctacaaactg gcgagcgaac tccagtataa gcgcattccc gatttggaga agcgcctggc
     8581 tgaactggaa aagattgaca aaggtgatca tcttctctcc gaagtcgtca ccgaagaaga
     8641 agtagccaaa gtcgtcgcca agaacaccca cattcccgtc agccgtctga tgcaggggga
     8701 ccgcgacaag gttctcggac ttaaagatac tttaaaaaaa cgggttatcg gtcaggatca
     8761 ggccatcggc ctcgtcagcg acgccatcgt aagacagcga gcgggcatcg ctaatccgaa
     8821 ccggccgatc ggctcgttcc tctttttggg accgacgggt gtcggcaaaa cagaagtggc
     8881 gcgcagcctc gccgaggctc tttttgacag cgaaacgcac atcatccgca tcgatatgtc
     8941 ggagtacatg gaaaaattct ccgtctcgcg tctgatcggc gccccgccgg gatatgtcgg
     9001 ctatgaggaa ggcggccagc tgacggaaaa ggtgcggagg agcccctact cgatcatcct
     9061 ctttgatgaa attgaaaaag cccatcccga agtgtttaac ctcctcttac aagtcctcga
     9121 tgacggacgc ttgacggact cgcaggggcg gacggtcaat tttaaaaaca ccgtcattat
     9181 tatgacgagc aatctcggca gcgaatattt catcgccggg aaaccggaga tggtcgacag
     9241 cttggtgaag tcgacgttca gacccgaatt tttaaaccga attgatgagc tcgtctactt
     9301 tcttccgctc gcaaaagaca cgcagattaa aatcgttgat aaactgctcg gcgaattatc
     9361 cacaaatctc aaatcgcaat atgtcgagct cactttcagc gaagcgctta aacgccatgt
     9421 cctcgacagc agcttttcgc tcgaatacgg agcccggccc ctcaaacgct tcatccaaaa
     9481 agaaatcgag acggcgattg cccgaaaatt aatcgatggc tcaatcgagc cccatagagc
     9541 ttatctcctc gatgtcgata gcggcgggaa tctgctcgtt aaaaatatct gagtttggca
     9601 attcgggcta taagcccatg aaaaaaaata ttctgtattt atcggcaatc attttacttt
     9661 tgtgtggtat actacataat tgaattataa ctatgccaaa ttgtcgcgga tgccatcgta
     9721 aaatcgaaag actagacaaa gatatttgtc ccttttgcgg cacgcctaat ccgattccag
     9781 gcagccagag tttaacggtc gatatcaccg gcgtcatctc cggcgcggga gtgccaaaag
     9841 acgagctgcc ccgggcctgc tcccgcaaaa gagcgtttac cttatgcgct ttgtttggat
     9901 ttttgggaat tcacgcgttt tatgtcaaaa aaccaaagca ggctctcttc tttatcttgt
     9961 tctctctttg tttaatcggc ggcgtcggct cgctcttgtt tttctttgtc ctgcccggtt
    10021 cgatctgggc tttcctaatt cctgtttttg ttcagattat atttcagatg attttcgcct
    10081 ttcattatct gactagcgaa gatttaaaag acggagtcgg agagctgatg cactgatgca
    10141 gcgctatttc ggacaagtat ttgaaggtcg cgccatcctc ggtgagggtg acgctttcca
    10201 tttagcgaag gtgatgcgct gccaaatcgg cacggaaatc gaagtcgtgg ccgacggtca
    10261 tgtttatcta gtcagagtcg aaacaataaa accgctattt ctgaaggtaa tcggcacaat
    10321 aaacgaaacc agagaactac ctaacgatgt cgttttggta atggcgcctt taaaaggcga
    10381 taagattaat ctcgtcttgc aaaaggcgac tgaactggga gtaaatgaaa tcgttttgct
    10441 cctcacctcg cggacaatcg tccgctttaa tcacaaggat atcatcgcca aactgacgcg
    10501 ctttagggcg attttaaaag aggcggccga gcaatcgcaa aggactcata ttccgaccat
    10561 cagctatgtc gacagcatct cgaaactcaa ttctctgacc gccaagcaca agtttgtcgc
    10621 ctatgaacag cagtcaggac cgactggcgg ttttaataaa ctcatcaaac agattaaaaa
    10681 acgcgagcga gttgccgtcg tcatcggacc cgaaggcggt tttgccggcg aggaagtcga
    10741 cgaacttaaa aacctcggct ttattcccgt ctccctcggc cagcgcatct tgcgcgccga
    10801 gacggcttcg atatatgctt taagcgtaat cgcaaacttc ttggaaaatt aatatgaaca
    10861 tcttcgaaga tcttcagtta aatcaaaaat ttcagggttt tctcgagagt gaggaaaatc
    10921 ttttttacct taaaaatcgc attatcctcg agcgctttcc gatgtacaag cacctggctc
    10981 aggccacgag cgatttcgat atcatccgcg ccaatatcat catccgctcc ttgcaaaaca
    11041 aaaaagcgat tgaggcggcg gtgcgctttg ccgtcagcaa taacgatgtc atcaaacgcg
    11101 aaaaagacat ggctttcttt cgcactttta tccgcgaaaa aatcatcgat ttgccatttg
    11161 ctaccgatca aaaaaaccgc ctctatgtcc cgctttttaa ccgcgtcatc aacagcatct
    11221 attctgagga gtttgaaaag ttgctcctct ctccttacga taaattggtc gacgagttcg
    11281 aaacgctggt cgtcgacccg tttgaaattt ataatttcag cctctttgat tcgctcttca
    11341 cccaattcgt caaagtatat agcgacgacg atatcatggc cgtttttcac tacgattttc
    11401 aagccatcta ttttattgat aaacaaggtc gtctcgacaa taaattagct ctttttgatc
    11461 gcgggctgcg ccaccccgat tttcaccatc tcctcgagcg cgttcgcccc gtcatcgccg
    11521 cctatatcgc ccacgataaa gaggcgatgc tcacggcttt ggccgaaaag aaacttatct
    11581 cggaaaagct gatcagccgc ctgcgcagca aagactacca attccgcatg tccttaattc
    11641 ggaaaatcaa gtgatgaact ttcttctttt tacccttggt tgcaaggtta atgcctatga
    11701 aagcgatgcc gtccgcgaac tttttttatc tcagggatgg cgtgaagcaa gaagcggtga
    11761 tgaagtcgag ttagtcgtca tcaacacatg taccgttacc ggtacggccg ccaaaaaatc
    11821 gcgccagcac attcgcaaat tccgccttgc ctatccgcgc gctttgctcg tcgtgatggg
    11881 ctgttatgcg caagaggaag gaacatctat catcgcggag tgcggcgccg atttgatcct
    11941 cggtacctcc catcgcaaag aggtcgttca attaatcaaa gaagtacaaa aacagccgct
    12001 cagacgggct gttatcgagg ccgaaccgcg ccagttcgat tatgaagaaa tcgcgactct
    12061 catgcagccg atgacatcgc gggctttcgt caagattcag gatggatgcg acaatttttg
    12121 ctcatattgc atcattccct atgtccgcgg aaaatcgcgg agccgcgatc cgcaagaaat
    12181 caaaaaagaa attcgcatgc tggtcgataa cggctatcaa gaaatcgtca tcaccggcat
    12241 tcacaccggc ggttacggaa gggatttagc gaattattct ttctcgcgtt tagtccgcga
    12301 aatatgcgag gaaatcaatg gcctttaccg cctgcgcatc agctcgattg aggcgactga
    12361 aatcgatgat gatttcatcg ctcttttagc atcatcttct gtcatcgccg accaccttca
    12421 catcccgctt caaagcggtt cggaaacggt cttaaaacga atgaatcgcc gctatcgaat
    12481 aagcgacttt atcaagacgg taaaccaagt gcgccaagcg cgacccggaa tcgcgataac
    12541 aactgatgtc atcgtcggtt ttcccggcga gaccgacgcc gaattcgacg agaccgtcgc
    12601 cttaatccgc gccatatctt tttcagagct ccatgtcttc ccattttcca aacgcgagaa
    12661 taccgcggcc gccaagatga gcgatcaagt ggagagcgag gtcaaaaaga aacgggtgaa
    12721 taccctgctc aaaatatcgg aagaactcca ttgcgattat gagcaaaatt actacggtaa
    12781 aacgctggat gttctcttcg aagattttaa tgaagccgac cagacttatc gcgggcatac
    12841 ttccaactac cttgaagtcc atctcaaatg ccaagaaaac ctcagtggaa aagtctcctc
    12901 aatcacatat cgcggaccga aaaattcaga gttaaaaaag gcttagattt tcgccctttc
    12961 ctattgactt acataagtaa tagattataa ttaacaagcc tacataagga gaaaaactat
    13021 ggccgtacca ttcagaagaa catccaaaac caaaaaaaga atgcgccgca cgcatcaaaa
    13081 acttgaagtc gaaggcttgg tcgcctgcaa aaactgcgga gcgacgatta aagcccataa
    13141 catctgcccc gaatgcggat attacgatgg caaaaaggtg ctcgaaacca agaaagccaa
    13201 agccaaagaa gaaaagcccg ccgagaaacc cgccaaagca aaaagcaaat agccctttgc
    13261 cctaaacgtg ttaaaataac ttcggaggaa ataccatgga atacaacaaa ttaatcgatc
    13321 acactttgtt aaagccagat gcatcactag cggccgtcgc taaactgtgc gatgaagcca
    13381 gcgaacatga cttcatgtcc gtctgcgtca atccctgctt tgtcccggtc gtcaaaaaat
    13441 tgctcgaagg cagtttagtc aaagtttgca cggtgatcgg attccctctc ggagccacgc
    13501 tccccgacgt caaggtcttt gagaccaagc gcgccgtcga actcggagcc gatgaaatcg
    13561 atatggtcat caatgtctcg atggcgaaag cccatgacta cgagttcatc gccaacgaaa
    13621 tcaacatgat caaagtcgcc tgccagggtc gccttctaaa agttattctc gaaacttgtc
    13681 tcctcaccaa cgaagaaatc gtcgcatgct gccgcgccgc cgtcaaagcc ggcgctgact
    13741 atgtcaagac atcgaccggc ttctcgagtg gaggcgccac cgtcgagcac gtcaagctca
    13801 tgcgcgatac cgtcggaccg cagatcggcg tcaaagccag cggtggtgtc cgcaccaaag
    13861 aagacctcga aaagatggtc gcggccggag cgagccgcat cggcacttca tccggtgtca
    13921 aaatcgtcgc caaataatta attaacgcat aaaaacttgc gaagggaggt gtgaacatgc
    13981 caaaaacagt cgtccgggac aatgagtctc ttgatgaagc gctgcgccgt tttaagcgcc
    14041 aagtcaacaa ggcgggcaca cttcaggaag cccgcaagcg cgaattctat ctcaaaccag
    14101 gtcttaaaaa gaaactgaaa agcgagatgg ctcgccgcaa aaaatactaa agacaaaatt
    14161 ggaagcgctg cttccttttt tatttcctgt tttgccgagg tttttcctta tatatcgttt
    14221 gagcgaatca tgcgatattt cacataccag ggaaacgatt atgactgtgg ctttgcctct
    14281 ttgaaaatgc ttctttgcta tgctcataaa accccaaaat atctgcgcct tgctaaacct
    14341 gaagaaaaaa ggagccgcta ttcctaccgc gatttgctgg acatcgccgc ccggcacggc
    14401 gtcgtcctta ccgcctataa gctaattgac aaaggcgaga ttaaaaaaca gaagaagtgg
    14461 ccaatcctcg tctcgctaac aagcgaaaac ggcagcttgc acttaatttt attaagaaga
    14521 attgggaaaa aagtgcttgc cgatgatccc gccagcggcg ccgtgacgat gacatacgag
    14581 gattttttgc ggcggtggga tggatcattt ctcgaagtta cgaaagttat cgaaccgccg
    14641 cgttttacga aggaaaaacc aatcgtcgcc ggatcgaaac gctttctcct tctcatcttg
    14701 cagtctttct ccgctctgtt tgccttaatg ggcttcgcct ttgtgtcctc ggaaaactat
    14761 ttcgtctatc ccctcttatt cttcgtcctc ttcatcgcct gcgaacttct ttttcggcgc
    14821 ttccagcttt ccgtcttggc cgaatttgac gagcgctaca tgcatcgcac ttttgatgat
    14881 gacaaactac ttatgcacac taaattcagc aagttccacg atttcaagaa agactatttc
    14941 ttatttccgc agattatcgt gagcgcgctg atcatctcgt gttttgccct gcttgttttg
    15001 gcattgaatg acatgcggca tctcgtcttt ctcgccactc ttttcgcctt tgcgttcttt
    15061 gattttttac tttgtaaagt tattgatgac aagcacgaga aactcctcag aaaattagag
    15121 gtcgattcct ttcgcgatat cctgagcaaa agcgatgcca tcagcaatta tcagaagatg
    15181 agttcactcg cccaactcat cgccaaaagc gcgattgccc gaaaatatgt cggcctcttt
    15241 ctcgtcggcg ccgcggcctt gacttatgcg gcgataagcg gggcggcaac gcttaatttt
    15301 tttctgttcc atttctttat ctatgcctta ttttacgagc atgtcggaaa acttcttaaa
    15361 tccctcctgc acatcggcga aatcaggcgc gagatagcgg ctttcaaaga ccaatttgtg
    15421 cgatagtata atatattata aatgtggtaa aataataata ttagggcatg agatggaaat
    15481 cacttttacc aataccggac catccggttt aaacgattat gaaactattt atcgcgattt
    15541 agcggtttcg gccatcgcgt ttttaaaaat caccgccgag tatatcatcg aggttaatct
    15601 tgtcagcgat gaagagattc aggctatcaa taaagcttat cgcggcaaag atgcgccgac
    15661 cgatgtcatc agttttgctt tcttggacag catcgagacc gaagtcgcaa tcaagggcga
    15721 tgttccgcgg ctcctcggcg aaattatgat ttcctacgag acggcggagc gccaagccaa
    15781 gaagtatggt cattctgtgc aaagagagat gaaatttctc ttcgcgcatg gtattcttca
    15841 cttactcggt tataatcacg agaccataga tcaagaaaaa gtcatgtttg ctttacaaga
    15901 tcaaatcctc gatcaggaga aaaaataatg aacagagaac aactcatcga attagccgtt
    15961 gaggccagaa gcctctcata ctcaccttat tcgcattttg ccgtcggcgc cgctttgcta
    16021 tgcaaagatg gttccgtcta tgtcggcagc aatgtcgaaa acgcctctta tccactctcc
    16081 atgtgcgctg agcgcaatgc cctttacagc gcctttatgg acggcaagag caaagaggat
    16141 tttgtcgcga tggcggtcgt cggcgatacc gattcgccga ttactccgtg cggtgcctgt
    16201 cggcaagtca tcagcgagct ctttccgatg aacgcgccaa tctacatggc taatttaaga
    16261 ggcgacgtca aagagacgac gattgccgaa ctgctgcctt ttgcctttga cggaagtgac
    16321 ttataaatga aatcgggttt tgtggcgatc atcggtcggc cgaatgtcgg taaatcgaca
    16381 attttaaatg cgctcatcaa tcaaaaagta tcgattgtca ccaataaagc ccagaccacg
    16441 cgagataata tcaagggaat ctatgaagat gacgaagcgc agatcgtttt cgtcgacact
    16501 cccggaatcc ataaaccgca tcacgaatta ggccgcatca tgaatcagag cgccctggcc
    16561 tcgacatccg gggtcgaagc cgtcatcctg gtcgtcgatg tcgccaagcc gtttggcgat
    16621 ggcgatgcct ttttggtcgg cgaattaaaa gtcgattgcc ccttaattat cgttctcaat
    16681 aaaattgatc tggccaaaat cgatcaagtc caaagcctca aagcccgcta tcaggaatta
    16741 tatcccaagg ctcatatcat cgagatgagc gccattcgtc tctttaatgt cgatgattta
    16801 ttaaaaatca tcaaatcaca cctcgaagaa ggtccgcgct actatccgct cggctcggtg
    16861 agcgaccgca atgaatcttt ctttattaaa gaactgattc gcgaacaagt tctgatgcag
    16921 ctccgcgaag aagtcccgca tcaaatcgct gtcgccgtcg aaaaattcca gttgaaaagc
    16981 aaggcgtcgt cgtcgaagct ttaatcatcg tcgacaagcc ttcccagaaa gccatcgtca
    17041 tcggtaaaga tggcgcgcgt atcaaggcga tcggcatcaa agctcgcgaa gcgctggaag
    17101 aacgcttcca taaaaccgct tatctgcaac tatatgtcgc gatcaaaaaa gattggctca
    17161 acaatccgcg cctcttaaaa gaatacggct acaaataatg gaaatcatcg gtattgtcac
    17221 gcgtttgacg ccctttaaag aaaaagacgc gatgatcaat gtcgtcgccg aaaataaggc
    17281 ttactccttt ttggcccgcg gcgtcttggg catcgccagc aaaaatatgg ccagcgtcca
    17341 gccctacacg aaatcaaggt tcgtccttac caaaggtaaa gaggggcttt ctcttcgcac
    17401 cggcaccgtc atcgactctt atgcgaaagc caaagaaggt ctagccggat tgacggcgct
    17461 ctcatttatc ggcgagctga caaacaaggc gattggaggc gaggaagccg ccaagatttt
    17521 tcccgctctt gggatggcat tagcgaacat cgatgccgga tttcatccgc tgaccatcgt
    17581 ccttttatat ttcgcggccg tcttgaaggc catcggcttt ggtctcgagg tgacaaaatg
    17641 cgttatttcc ggtcagagaa gcgatatttc ggcgatttct tacctcgacg ggggatttgt
    17701 cgccacgcct tatttcgacg ctctgcgcca ccagaaacga aatgagcggc aactcaagat
    17761 aatccgccag atttttctcg ctgaggccac ggactttgat cgagtcgctt ttacaagcgg
    17821 cgaatgctta gaaataatta aagatttatc ggtttatatt caggatggcg ccggcatcaa
    17881 tcttaaaagt atctttctac tggaaaaatc ttcataaaat cataaactac acttataata
    17941 ggttgacaac acgcccatga gagcatacac ttgggcggct tgcgttttac gcggaggaag
    18001 acatatgaaa tatgatttcg acacaatcgt gaaccattta atcaccagcg gttttgtcta
    18061 tcaaggttcg gaaatttatg gtggtttatc gaatacttgg gattatggtc cgctcggttc
    18121 cgagttaaag aataacctta aaaaggcgtg gtggaaaaag tttgttcagg aatcgccgac
    18181 taatgtcgga atcgacgcct caatcctcat gaatccccgc gtttgggagg cgaccggaca
    18241 cgtctcggct tttaacgatc cgttgatcga ctgcaagagt tgccggtccc gccaccgggc
    18301 cgatgattta atcagcaaag cccatcccga aatcaatgtc accggtttaa caaacgaaga
    18361 gatgaacgtc cttatcaaag ataagaagat cgcttgcccc gtctgcggca aatctgattt
    18421 caccgatatt cgccagttta acatgatgtt taaaacccat atcggcgtcg tcgaagattc
    18481 gaagtcggtc gtctatctcc gccccgagac cgcgcagggt atcttcgtca atttccgcaa
    18541 cattcagcgg acgacgcgcc gcaaactgcc tttaggcatc tgcaatgtcg gcaagagctt
    18601 ccgcaatgag atcacgcctg gcaatttcat ctttcgcacg cgcgaatttg aacaagccga
    18661 actcgagttc ttctgcaaac cgggaaccga cctcgagtgg tttgaatact ggaaaaacta
    18721 cagtctaaaa ttcctcagga gtttaaatat caatcaggac aaactgcgct accgcgacca
    18781 cgattcaaag gaacttgctt tttattcaaa agcaacatgc gatatcgaat acgaattccc
    18841 gtttggctgg ggcgaattat taggcattgc cgaccgcact gactacgatt tgaaacggca
    18901 tatggaatat agcggtgaat cgcttgaata cctcgatagc gacagcggcg aaaaatacgt
    18961 tccctactgc atcgaacctt cttttggcgt cgatcgccta atgctgacgg tgatgtgcga
    19021 ctcttatgtc gaggaacaac tcgaaaaaga tcagcgcgtc gtgatgaaac tccaccccgc
    19081 tttagcgcct tacaaggtcg ccgttttgcc tttaagcaaa cagctcaacg aaaaggccaa
    19141 agaagtcgtc aatattctca ataagtattt cgccgtcact tatgatgaga ccggaagcat
    19201 cggcaagcgc tatcgccgcc aggatgcgat cggaactcct tatgcggtga cagtcgactt
    19261 cgaaaccgcc gacgaccaga gcgtcaccat tcgcgatcgc gatacgatga atcagattcg
    19321 cctgccgatt aaagaactcg tcggatactt cggcgtcaaa ttgttctatt agtattaaac
    19381 tagtatattt ttagtttaga aaggaggagc ccatatatta tgatatatga atcgagttta
    19441 gttgatgata ttctcaagca agcagatatt gtaaatgtca tctcaagtta tatcaacgtg
    19501 attcaaaaag gtcgcaatta tgtggcccta tgccctttcc atgatgacaa gaatccgtcg
    19561 atgatgatct ccaaggaaaa acaaattttt aaatgctttg tgtgcggcgc cggcggcaat
    19621 gccatcggct ttgtcgaaaa atacgagaag attccttttt cctccgccat tcgcaagacc
    19681 gcggaaatca tcggttttca agacataagg ctcaataagc caatcagcca cgtgcaagtc
    19741 gatgagacga tcgccccctt gcatgagtgc atcgaagact tgacgactta ctatcagttt
    19801 ggattatcga ccgaggaggg ttcaaaagcc cgcgcctatc tcgaagggcg tcacatcgat
    19861 ctcgaccagc aggaacgctt tcgtctcggc tatgcgatta aagacggaaa aggaaccgtt
    19921 tcatatttgc agcaaaaagg atttagtcta aagactatcg aaggcatcgg catcgctttg
    19981 gccaaggcgg aaggcacctc cgatttcaac gccggacgcc tcatctttcc gattcacgac
    20041 ggcgatggta aagtcatcgg ctacagcgcc cgcaaactct tcgatgaaac cgaaggtccc
    20101 aaatatgtca actcaccgga aaccaaactc tttcataaaa gttccgtttt ctataatttt
    20161 cacaacgcca aattaaccgc caaacacgac ggctacctct atgtcctcga aggcttcatg
    20221 gatgttttcg ccctcgataa aatcggcatc aaatcagcga ttgccatcat gggaaccgcc
    20281 ctcactcgcg aacaggccag catgttgagg cgtttaaatg tggagatacg cctctgcctt
    20341 gacggcgatg ccgccggcca gatggccatg atgcgcatca tgggcgtgct cgatgccgcc
    20401 gacgttaaat atcgcctcgt ctccgagccc ggaaatgaga aagatcccga tgaaatctta
    20461 caggaagacg gccccgaggc tctgcgtaag tatcttaata tgctcgtcga tccctttgag
    20521 tttgccctca actattatca gcacacgaac cctttgggtt cgctggagga ccgccaaaag
    20581 gttgtcaatc actttttgcc gatgctcgtg cagttaagta aaggcatcga cctcgacaat
    20641 cgcatctttc gcctttcgaa agtcacgcgc tttgatccgc aggcgattgc caagttcgtc
    20701 gacgaagcgc ggagccgcgt aagcaccgaa cgaccgatga taaccgaact cccggagatg
    20761 gagttcaaac cgttcaggaa agatatacgg cgtctgcaaa atgcggaaag acaacttctc
    20821 tacagcatgt tcaaggataa aaatgccgtc gatttctatg aaaaaaacgt ccagtacttc
    20881 tatatcgaag tgtatcggca catcgccctt catatcatcg actacttgtc gaagcatgaa
    20941 gagatcgacg tctcgctgct catcaatgag atcgccatga aagaggcgcc aaataaagat
    21001 gagatcatcg acgagatcac gagtctgaat gctaaaaaca tgtatccgga tccgactgag
    21061 caaagcctta atgaaatgcg cgatgtcatc aaggaggaga cgcaaaatct ctacgaaaaa
    21121 gaagcgctta aaaaatcgtt gctcggcaag acaccgcgcg aacaagcccg catcatcgac
    21181 gattataatc gtcgtaaaat gaaaaaaatc ggaaaataac cgataggaga aatatatggc
    21241 taagaaaaaa atagatgaag aagcggtcgc taaagacgtt gaggcggccc aacccgccag
    21301 gagcaaggtc gcccagacgc ctaaaatcaa aaaaggcggc gaagtcaaaa aggatgcaaa
    21361 agttaaaaaa gaaaaagtga aggccaaaac cagcaaaaaa atcgataagg tgagcgacga
    21421 agaggaagaa cccgtcatca tcgtcgacga agaagaggaa atcgaatcgc tcgagacgat
    21481 taaaaagcgc ctcgtcaaaa catttaaaga acgcggattc atcgatatga gcgaagttct
    21541 tgatgaaact tctcacctcg atttatccga tgccgatgtc gaagatttaa tcgtcttttt
    21601 gaagagcaaa ggcgtcgatg tcatctccga tattgatgcc gaggcggaaa tcgatgatat
    21661 cccggaagaa gaaattgcca agggcgactc gcttgtcgac gaagatctca tcgatgaaga
    21721 agaagtcgtc gatgaagaag tcgaggcact ggccgagatc gacgatttat caaagctcgc
    21781 caccggcgaa gtaaaagtta acgactcggt taaaatgtat ctcaaagaga taggtcgcgt
    21841 tgatctattg cggccggaag aagaaccgat tctcgccaag cgcattctcg aaggcgacat
    21901 ggcggccaaa aatgaattga tcaacgccaa cctgcgctta gtcatcaaca tcgccaagca
    21961 ctatagccgc ggcaaagcga tggactttct tgatttgatt caggaaggaa atctcgggct
    22021 gatgaaggcg gtcgataaat tcgattacac caaaggtttt aaattctcga catacgcgac
    22081 atggtggatt cgccaatcaa tcgcccgcgc catcgccgat caggcccgga cgatacgcat
    22141 tcccgtccac atggtcgaga ccatcaacaa gatgaaaagg gtgcagagac agctcgtcca
    22201 gcgctttggc cgcgagccta ccgccgagga aatctcggag gaattagaag gctttctctc
    22261 gccggaaaga attcgcgaaa ttcaaagaat cgcccttgat cccgtctctt tagaatcgcc
    22321 aatcggcgag gaagaagatt cccacctcgg tgattttatc gaagataaag aagccgaaac
    22381 acccgtcgaa ttcacgacca aatcactgct taaagatgaa ttgtatgaag tcatgaaaga
    22441 tttaactgac cgcgaggagc gcgttttgcg cctccgctac ggattagatg ataaccgccc
    22501 ccggactctt gaagaagtcg gaagggaatt tggcgtcacg cgtgagcgca ttcgtcaaat
    22561 cgaagctaaa gccattcgca agctccgcca tccgacgcgc gctaaaaggc tgggcgattt
    22621 ccgcgactcc cgatgatcag gttaagcaag cgcctcagcg ccatcgccaa ctttgtcgat
    22681 aagtgcgccg tcgtcgccga tgtcggttcc gatcacggtt tactcctcat ctatctggcc
    22741 agcctcggca aaatagccaa aggctatggc atcgaaaaca aaccgggtcc ttatcaaatt
    22801 ctcaaacaca atctcgaaag ccatagtgaa gcggagttaa agcctctgct tcaggatggc
    22861 atttcaaacc ttgaaaagga tgtcgacacg attgttttag ctggtctcgg gggcgacacg
    22921 attgtccgca ttttagaaaa cggtgtcgcc aatctcaaga atgtaaaaac aatcattacc
    22981 gattcgcaca cgtcgatcgg cgaagtccgg cgccacattg tcgctctcgg ctttatcatt
    23041 aaagatgaag tattagtatt agagaaggac aaatattacg aaatctgcaa attcgtcaaa
    23101 agcgatgaac gccccgctta ttctgatttc gaatatcggc gcggacccat catcatcaaa
    23161 agtccggcgt ttcgtcacta tgccaaagtg ctaattaaaa agatggacgc catcctcgaa
    23221 aaggatttgc ccgaagccat gaaagagctt gtgcggcaag aaagagaggt tttactaccc
    23281 tatgaaaact aaagccttag tccgcaaatt agcgacgcgc tttcctaagc gcatcgccga
    23341 acccggcgac cgggtcggac tcatgacagg aaaatttaaa gagaagacaa agcgaattgt
    23401 cgtctgcctt gattttgatg aagaagtcat gcgccacatc gaagcccttg atggtggtcc
    23461 ggtcgactta attttgacac accatccctt cttatatcgc tcccgctatc aagtctttaa
    23521 aaaagatgag atcaaacgga gattgacgga gaaagtcgat gccctcggca ttccggtcta
    23581 cagcatgcac acgaactttg acagcggcaa agacggtatg aacgacgctt tagcgtccgc
    23641 tcttaaactt tctggcattc gtcagcttga gagcatgccg atggcgcgcg gcggacgcct
    23701 ggctcagaag atgccgatta agcaattcgc cgaagtggcc gcccgcttgc taaatgttcc
    23761 ttacggactt ttaatcaatg ccggcaagga gatggtcgag acggtggcca tcatcggcgg
    23821 cgggggcagt tcatattatc aaaatgccct gaatgaaggc tacgacatct ttattagcgg
    23881 cgacgctccg caccacgttc ggcgcgacat tgttttacga gagtataact atcttgatct
    23941 gccgcatgaa atcgaaagaa tttttatttc tcagatgggc aaaattttaa aagaaatcga
    24001 cgctgattta gaagtgattg ccatcaatca cgaagagacg ccagaagtaa ttatcgtctc
    24061 ttaagttctt cgaccacgcg attggtggtc tcaagcggcg tcgaagcgcc ggagacgact
    24121 gcgacataat taagaccatc aaggtctttt ttatttattt catcagcgtt taaaatgcgg
    24181 atgacgcggg tttggggcag cgtcttgcgc gccatgtccg ccaaagaggc ggtgttgctg
    24241 ctgtttactc cgccgatcac atagacgagt tcagtctctt tgggcagcgc caggacggcc
    24301 tgctggcgga ggcgcgtggc gttgcatatt tcatcggcga tccgcgcctt agaaaactta
    24361 ctgataatcg aagtgtgcaa agccgccaat tccgtctgcg aaagcgtcgt ctggttgatg
    24421 actaacggcg atttgtcgac gagctccaaa agattgacat cctcgttatt ttctataaga
    24481 aaaacattgt cgccgagcga tttggcggca ttggcttcag gatgattttt cttgccgata
    24541 taaataactt gatgatcgtt attgagttcg ttttgaataa tgcggtggtt ggtcgtgacc
    24601 atcgggcaag tcgcatcata gacgaccatc tttttttggc gggcgatttc atcaagagat
    24661 acttcgtggc cgtgagccgt aaatacgaca accgcctggt cggggagttc ttttaataat
    24721 tgcgccggcg attttttcaa atcgcgaacg gtggtgatac cgagggaaaa aagttcttga
    24781 atgacatctt cgttatggac gatgctgcct aagacaaaaa ccggtacacc gacatgcttt
    24841 tgacgcgctt ttttgacgag attgatcgcg cgaatgacgc cggagcaata accgtggggc
    24901 cgggatatga atatattcat gtgtttataa tagcataggc aaactcattc cgtgtataat
    24961 tatcaaggag cgaaaaatga gctttaaatc ttttaattta agcgatctga tgatcaagag
    25021 tcttgcccgt caaggctatc tttcgccgag ctcgattcaa acccgcgtta ttcccaaaac
    25081 tttaaaaggt ggcaacatcg tcgcccaaag cgagacgggc agcggcaaga cgcatgcttt
    25141 tatcattccc atcatcgaaa atcttcagct cgatagcgac cgcctgcagg cgctgattat
    25201 cacgccgaca cgcgaactcg ctcagcagat ttatgccttt ctcgaagctt ttaaaggcga
    25261 ttatccgaaa ttgcacgccc gcttgtttac cgccggcaaa gaaacggagc gcacctttcg
    25321 ccaggggatg cgcgttcccc acgtcatcat cggcaccccg acgcgcataa agacgatgct
    25381 cgaagccaag tctctgattg atctcggctc ggttaaaaca ttagttttag atgaagccga
    25441 tatgctgatg gagatgggct atttcgctga tattgatatt atttaccgcc tcttaaaggc
    25501 gccgccgcag atgatggttt tttccgcgac gatcgaattt aatctccgca agaatctcga
    25561 aaaatatgtc ggcgctgatt tcgtcatcga gatggatgat gtcaaaaccg ccaagacggt
    25621 cggccaccac gcgattgacg tcaagcacgg cgacagactc cacgccatcg agatttttat
    25681 caaaacctta aacccttatc ttttaatcat tttcgccagc cgcaaagaac aagtggccac
    25741 catcagctca tacttaaaaa gcgccggaca tgataatatc atgctccacg gtgacatgag
    25801 cgcccgtgag cgcaagagcg cctacaagtt gatcggcgcc aacaagcatc gcctcatcgt
    25861 cgccagcgac ctcgccgccc gtggcctcga tatcaaagat gttagcgaag ttctcaattt
    25921 cgacttgccg aaagacctga cctactattt tcacagagcg gggcggaccg gacgatttgg
    25981 cgccagcggc caatgctaca gcttttataa ttccgatacc gtcacacaac ttgaaaagct
    26041 catcgagtca ggagtgtctt tctcctttca tgaactccgc gatgatacaa ttatttcgac
    26101 cgataaaatc gctcgccgca aaacgagaaa acggaaggtt gatcccgatc ttgagcggca
    26161 gattaaactc gcggcggcca aagtcaaaac cgatgtcgta aagcccggct ataaaaagaa
    26221 ggttcgcgat gcggtggcca aagttaagcg caagcacaag cgggaaatca tccgcaagga
    26281 tatccgccgc cagcgcgtgg caagatatcg aaaggaaggt agtaaaaatg agtaaactgt
    26341 ttattggatc acatgtctcg atgtcgggac cagaatatta tttaggcagc gtcaaagagg
    26401 cgctttctta tggtgccaac acctttatgt tttataccgg agccccgcaa aattcatatc
    26461 gcaaaccggt cggagaacta aagattaagg aagggcgcaa ggcgattgcc gatgcgggta
    26521 tcgatgagag caaaatcatc gtccacgcgc catatattat caatatcggc aacaagacaa
    26581 atccgactgt ctatgaatca tcgaagaaat ttttaatcga ggagttacgg cgcgtggccg
    26641 cctttggtcc gcgcattctt gtcctccatc cgggcagcca cgtcggaatc ggcgtcgaag
    26701 ggggtttagt ctcggtggtc gaagctcttg atgaagtgct ggagcaggac ggaacaaatg
    26761 ttcgcatcgc cctcgagacg atggccggca aaggttcgga agtcggtgcg actttcgaag
    26821 agattagcta tcttcttacg cacagccgcc atcccgaacg cttaggtgtc tgcctcgaca
    26881 cctgccacat caacgatgcc ggcatcaacg tccacgaagt cgacgaagta ttaaatactt
    26941 ttgaccgcgt catcggcctt gaaaaattgc tctgcctcca cgtcaatgac tccaaaaatg
    27001 tcatgggcgc ccacaaagac cgccacgcca acatcggcta cggcgaaatc ggctttgaga
    27061 cgattcacgc ttacatcaag catccgcgtc tagaaaatgt gcccaaaatt ttggaaacgc
    27121 cttatatcgg tgaatacgcg ccttatcaaa aagaaatcgc catgctccga gagggcgtct
    27181 tcgaggaagg ctggattgag aagttataat ttttaaatta taagagaaaa ggacatcagg
    27241 tgatgtcctt ttctattgtg gcaatcaagg catcaaaaac ttcatcctct ttgactgtgt
    27301 gaagaagttt gcctttttta aataagaccc atatgccgtt gccgcccgcc atgccgtaat
    27361 cggcgtgtct ggcttcaccg gggccgttga caacgcaacc catgacggcg actttgagat
    27421 taagattatg ttcttcaaga taggccaata cgcgcttagc catgggcacg acattcacct
    27481 gtgtgcggcc acaggtcgga cacgagatga tggtcggata gcgctcatat aaatccagat
    27541 cgtgaagcag ccgtttagcc gcaagaatct cttcttcggg ttcgtcgcta agggagatgc
    27601 gaatcgtatc gccgatgccc tcaatgagaa gcggcgctaa accggcggcg cttctgataa
    27661 ggccgatatc tttaggcccg gcttcggtaa ttcctaaatg gagcgggtag gggaaggttt
    27721 tggctgcctt gcgataagct tcgatcgtct caatcgcgtt ggagccttta agtgatatga
    27781 ctatgtcatg aaaaccatat ttttctaaga tggccacgtg cttctgggcc gaggccacga
    27841 gtttttcggc atgcgaaaaa actgagtttt cggcgatatc tttatcgagc gatcccgagt
    27901 tgactcccac gcgaatggga acgcggtgct tctgacaggc tttgacgaca gctttgattt
    27961 tatcttctga gccgatgttt cctgggttaa tgcggatttt gtcgacgccg ttttcgatgg
    28021 ccagcaaagc taaacggtag tcgaagtgaa tgtcggcgac gagcggtatc gagatttgct
    28081 ttttgatttc gcgaatcgcc gcggcatctt cctcgtcaag aactgaaacg cgcatgagat
    28141 cagcgcccaa aagcgtcacg cgcctaattt gggcgacaac ctcggcgatg cgactcgttt
    28201 taatgctgca catcgactga attaaaacgc ggttttgacc gccgatggcc cggtcgccga
    28261 tttgtatctt tcttgttaat tctcgcggta acataattac ttatataatt atatcactcg
    28321 ataacttagt gtagatatct ttatttatat gtgctactat ttatgagcaa tttaaaaaga
    28381 gg
//
LOCUS       DAOSSL010000009        27535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1924_length_27535_cov_17.920706, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000009 DAOSSL010000000
VERSION     DAOSSL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_27535_cov_17.920706"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1956
                     /locus_tag="PLH17_01810"
     CDS             103..1956
                     /locus_tag="PLH17_01810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20551.1"
                     /translation="MNTFKNLELIDKERINRLVRDSFYYSAYSSSELVFENMFVWNFN
                     RQIEILWLDEDLALVRSFEKDNRWIFFPPICRTPDKFKQGLTHIKNNYPSALVGGLSN
                     DMLAIASIEGALYLYDDYYSEYIYDPVELAEMIGGKFSRKRNLIAQFKKKYSYAFAPY
                     TDEYLQAVKDFLYRYEQEGGAGEDFDAILYALNNREKIDLFCDLLLVEGLVVGLSIGT
                     ISVFNHAVILFEKNDFNYVGSGAVLVQLTSDAHYRNCRVLTRQEDLGLPQLRKAKLAF
                     NPIEKERKYSCFFDNATIQLYHLYLSSFEDSRDYVDFFFLHYYHPERAFGVQRDGTIV
                     SALHIIMKQMIYNNKIIDMPFIVAASTDQKYRRQGLMREVMAKTFQALIAEGQTIVSL
                     YPVNPDFYRDYGFVQYTFTRDINSYAKSFECGLEQTSDAALLARMYDKCISEYEGYVV
                     RDEQYYTRYMNSLWQDGYVFELIKKAGEVVGYLVRKDEDISEILLCGEEKPMHKDLDT
                     TDTFMPHPDGDIPSNMIRVLNVEKLLLSMSFDRDTSFDLRLKITDIFVNTNNLVLDLS
                     VQSGRLVLSRCDNYDFEVSIEALTEALFTGRGDDRLAFLFPHKKMVCFDKF"
     gene            2045..4525
                     /locus_tag="PLH17_01815"
     CDS             2045..4525
                     /locus_tag="PLH17_01815"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOR20552.1"
                     /translation="MKKIVIVGGVAGGATAATRARRLSEEDAIIVFEKDEHVSFANCG
                     LPYYIGGVITDRERLLVETPETFKSNHNIDVRTFSEVIAVDTKHKKVTVQEVKTGRTY
                     EETYDKLLLSPGAAPIWLPIPGIEKAHNVFSMRNLVDTDKLFNFITKEKVESAVVIGG
                     GFIGVELAENLVERGIKATIVDLADQILPPLDFEMAKMAQNELETNGVKVYLKDSVAE
                     IKDNGKTAVLKSGIVLKTDLIIMAVGVRPESRLAISGGLKVGPKGHIVTNEHLQTIDA
                     KSGEVIPDVYAAGDAIEVKDFIDGTPTAVPLAWPANRQGRLVADHMNGWDVRFNGSLG
                     TSIVKVFSLTAASTGNNQKTLERKGVAFRAVMVTRSNHAGYYPGASDIVIKLLFSPES
                     GKILGAQAVGQDGTDKRIDVIATAIKGGLTIFDLPDLELAYAPPFGSSKDPVNIVGYV
                     GSNMMQKEFGVVNQNELDEFRKKKALLIDARTPLEYSIGHIQDSVNIPYYGVRHNLDK
                     LPKEKDAPIVVYCNVGHTAYLFIKVLVNHGYTNVHNLLGGYKIYKALHQKNHDEELHN
                     PEHRVIEEKTAVNSTDYREDEIKIFIDACGLQCPGPIMQTYKAVEKLKDGEIVKIIAT
                     DSGYFSDVEKWTVATGNTLLKKEATKEGYTAVIRKGSLKPVQKAVENENTTIVLFSGE
                     MDKAMAAMIIGQGSRSMGKNVTIFCTFWGLNLLRKDQKVKVKKSFIEKMFGAMMPRGA
                     KKMPISKMNFGGMGAKMMKSVMKKKNVDSLETLIAQAKAAGVKFIACTMSMDVMGIHR
                     EELIDGIEYAGVATYLAESEKAGVTLFI"
     gene            4663..5799
                     /locus_tag="PLH17_01820"
     CDS             4663..5799
                     /locus_tag="PLH17_01820"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HOR20553.1"
                     /translation="MAIIHLTDYVRKENAKLKLGTFRDVDALVFAQLSYLDYSPYKNG
                     IKLEFTAEKAKEAARPSHMKEENEDLLRCAAKSLRYQHIKVCRHVAETNLLFELQFSA
                     TTFLISPRFIVIAFRGTDDTINGWKEDFNMVFLDEIPAQTKAVNYVKAIAKKYRFARI
                     LITGHSKGGNLAFYAAMKQSKAVKKRIVSIYNLDGPDFRENITASADYIDLLPKVIKI
                     VPEESIIGMILQTNDYYHIVKSEGTLFAQHKMYNWHIDSETSDLVHEMSLSKSSKKIK
                     NSLYAWLYSFNVHDRQIIIDNFFDMLQASECTTLSAFSSNLSGNIKAMYGEYKESDSE
                     TRKLIRKTFIELAKYLMSNYLTREKDLDKKAKKFALKRKIKEGE"
     gene            5801..6262
                     /locus_tag="PLH17_01825"
     CDS             5801..6262
                     /locus_tag="PLH17_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20554.1"
                     /translation="MPSSTIVQLIKYTESLSNREMMKWALRNVREVLDVLNINVTNDE
                     QYRLMVIETNRFLTGFHKVPHMRNQAFKMHELARHENDPVRVNLLRAYGHIIATAHVK
                     EHALHATEYANKALRAANKSEEDIEKIVDAQNNALESLARFIVSVDRPIVN"
     gene            6314..6892
                     /locus_tag="PLH17_01830"
     CDS             6314..6892
                     /locus_tag="PLH17_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOR20555.1"
                     /translation="MNTTILLAHPWHGSFNKAILDTVTGKLANLNKSYQIIDLNKDGF
                     NPVMTEKELALFSRGQFSDPLVGKYQKMLADTDELVVIFPIWWGDSPAILKGFIDKVM
                     LKGFAYEQGASGLIGKLTHIKKGYVITTSETPTFVYKEHLGNPIDSIFLKIILQSIGV
                     KNNVWINKDMVFSGGQESRVAFLKEIAGLFDK"
     gene            complement(6974..7480)
                     /locus_tag="PLH17_01835"
     CDS             complement(6974..7480)
                     /locus_tag="PLH17_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOR20556.1"
                     /translation="MTKLKKRVITTPGIIYLSLFVAFATVILVFSAFSGEISSSQSNF
                     VVDILNSILRFFGVILNESQLDTFAFFVRKIIGHFLIFLLDGIFAYLMLINLSIMKKK
                     WLPVILAISLMIMIAGASELIQLFTSGRSGNFYDVGIDLSGAMLGVATAFLVTLSRKE
                     KAEHSSAS"
     gene            7589..8002
                     /locus_tag="PLH17_01840"
     CDS             7589..8002
                     /locus_tag="PLH17_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOR20557.1"
                     /translation="MVFVKYLILIALTFVVFLVFDLVWLIKISPRFYKKNLGHLMAEK
                     VNYAPAIIFYLIFIVGITLFVIGPAYVGGRGWSYALIYGVVFGLVTYSTYDLTNFATL
                     KKWPLNVTLADIAWGSFLSTATSISVYYLAALMGV"
     gene            8006..8818
                     /locus_tag="PLH17_01845"
     CDS             8006..8818
                     /locus_tag="PLH17_01845"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOR20558.1"
                     /translation="MAILGYVLPPVLVILFFTITYFVAETKKNYGYIDIVWGASFVIS
                     AWSALVITSLVNMAWPSIPAIVVAVAVTIWGLRLSTYLFKRNWNKPEDYRYVTMRNNL
                     AGKKHPKLLAFIKIFMSQAGLSIIISTVILVTLNGVVIANDWLVYIFVIAGGLLWIIG
                     FFFESVGDAQLRSFIRKPENRGKIMDRGLWGITRHPNYFGESLMWFGLGVVALAVAYG
                     YIGFASYIIITSLLLFVSGVPLLEKAFKDRPGFTEYAARVSIFIPWFPKKKK"
     gene            8837..9679
                     /locus_tag="PLH17_01850"
     CDS             8837..9679
                     /locus_tag="PLH17_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOR20559.1"
                     /translation="MKKVHIITDSTSDISQQQAEKLGIDVVPLHVNIDGVDYIDGLTI
                     SNQDFFKKLRTAKILPKTSAPSPGSFLDVIKKYPKDEEILIMTISSDASATYSSAKLA
                     VEEAERDNIVLYDTRQATFGLLALVHAAIQFRDVGDNAKTIVEKVQEISRRIVVQAAF
                     DDLRYLRMGGRIPFTLAILGSVINMKPIITMQDGKIHSAAKIIGMQRALNHIVYNLAD
                     NNVDYSLPTFVGHTDNHEMFKRFTDLIKIHKPEFPLDREASVGLAVGTHAGPGCVAIV
                     YFTK"
     gene            9741..10637
                     /locus_tag="PLH17_01855"
     CDS             9741..10637
                     /locus_tag="PLH17_01855"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOR20560.1"
                     /translation="MKHSKLLGLLVLLVTYTAAFFVGLIIFNLFKSWVPDVLMRILIA
                     DVIATLFVWSVGLVFKTASVYDPYWSVQPLVILLALMIYRGVFDLGAIVLLSLVALWS
                     LRLTANFIYTFSDLSYQDWRYTMLREKSGKLYFFVNLFGINLFPTLVVYACIYPMLLY
                     VERGGSATFNLLSLIGIILAVIAILFETVADVQMQQFRSAPENKGKIIRVGLWKYNRH
                     PNYFGEILMWWGIYAVVLALNPSIWFVFFGALINTLMFMIISIPMAERRLAGYKAGFN
                     EYRDETWMLFPLPFAKGKRREQ"
     gene            10688..12115
                     /locus_tag="PLH17_01860"
     CDS             10688..12115
                     /locus_tag="PLH17_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20561.1"
                     /translation="MDLKKRLYALVDKYDALIIFLVIECLALTAFSLADANIIFRYLG
                     FLISLVLIPFALMFTSKREWLSLGLFSIPLVIIAFLTGFSTFTLSMGFGSLDNLSVVL
                     GTLAFFFLGFAVRRVRKFKIDIALLAIGAAMALLVFLGLIYSLYQYGPFHASLYEGLV
                     YYYDGRLFVVSEETKWLLGFSFVEISINYVSLFGAMLSTALTALLYISPKTEPRKFAI
                     VAVIGLIGLLSIALIPNIEALILLIPVFLFALGYRFIRSREKVGIIVQYAFFALLGIA
                     GIALLVVILNNAGVSFIRNALAGNAFLNRLFNTNRIMSPVNAVLAPMFTAGGFFGFPI
                     TYIDGLVANTGAFEFEIVKEGGLLAFLAIIAFLLLVIFSLARYSAKSPDKGHIKIVIL
                     SLLIAALLNGSLLWDTFPLIYESDYYFSFFRSAPYLIVLFLIGYAYYPLSGKPLIEEP
                     EEIVLVSETTNADHLANSDEEEIKL"
     gene            12112..>12347
                     /locus_tag="PLH17_01865"
     CDS             12112..>12347
                     /locus_tag="PLH17_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20562.1"
                     /translation="MRKKAAFALLFTFAFVLTGCMNDVAELYEGHAYDTGVFSENYYS
                     TWNDSLKNSVAPVAKTYEVTAANSFQIEQDSDFA"
     assembly_gap    12348..12447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12502..13437)
                     /locus_tag="PLH17_01870"
     CDS             complement(12502..13437)
                     /locus_tag="PLH17_01870"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOR20563.1"
                     /translation="MANPNLQENKLKRALYAPFRGIIKKLFPVFFVKQQYKYITHHKL
                     NLEKPLRYTEKLQYLRLFVYPHDDLVIKCAGRAGVREYVEDRGYGASLIPIHGLYNHF
                     NEIDFAHLPSAFVMKATHACAYNVIVRDKANLDLIPLKKRFEKWLKTNYGQKTLELHY
                     APIKPQIIIEKYIGEVDSLPVEYKIHVFNGQARSMYVVTGRGADIRYNNYYIDWKPFD
                     GSQFNGWKKRDELIPIPENWIEMVKMAEDLAKPFPFVRVDLYNIDGKIYFGELTFTPA
                     KGTLILDDDRCDFEMGAWLNIEPYLVHHPKLKKKI"
     gene            13526..13870
                     /locus_tag="PLH17_01875"
     CDS             13526..13870
                     /locus_tag="PLH17_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20564.1"
                     /translation="MKKALRSWLTYVVAGALFLVAAGIAVIPDIVDNYVATMEEWYAH
                     YIFLFIGIALIFVGFVWQDLLKAQHRRKTKNWDGPLSEDVTNGAWAVSLPFYLAAVLS
                     IIGGIIFTFVAI"
     gene            14045..16426
                     /locus_tag="PLH17_01880"
     CDS             14045..16426
                     /locus_tag="PLH17_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR20565.1"
                     /translation="MTFPKKTALLLAALVLVVPLSSCDGGGSDKFYVTGPYFTGYEAK
                     DVYNAYLGSSISTLNSAKSQSATDVRHLANFVDGLVMNNEFGILERQLASSASVNTEY
                     TQFDFTIKQNIPWFTYDGAQYVAKNTEGQDVPQFVKADDFVNTAEIILNYANESEIAY
                     MVTMFIDGGWEYYCYTEMLKLIKDRNTNYAGINWMTLKSASNDAKATALMTLIAYESG
                     MDPIDVPLILGEDLESIANFSRVGVKATSEYGIQYRLNQPARFFPTLLTYAPFYPINR
                     AFFSQHKFAGFGTSKEKLLYNGPYLCSEWSDTKVQYTRNENYYNVEDVHIKTVNYMVA
                     PDTISYAEMREDFENGVIDGFTIAKEDSVGWAQYITGGTPEAPGSGTVQNPYSPLVNS
                     RELNTIDYTWHFTLNVERETDVSLQPNTIFGGESPDYPASAIANTNKAMKIKEVRRLI
                     LDGIDFEQYNQTNTNTEDIHQYQLNTLTPPGYVFDENDKDYLEYYYEEYAENKGLENG
                     AAAKALIGPQKIEGVNYSDAEVLTMRAEAADAVAQYNALNPTDPITTPIIVENAGLAG
                     YAGTSYITAEARWINDFNDRVNSGLAEGNEFLTIVNNLKATTSDKASNNAYAAYYTLN
                     SWGWIGDYADPLTYLHTYVTNGDMSLFTGTKAARDNYYLNASDELVYEEDGLLEDYDD
                     LVDDAKAITSSNNLRYDAFAKAEYELINDVYIIKPHSMNSQGWAVSVSRAIGYENPTA
                     SYGLADYRLTGMWVLVEPPTGAERQVARDLQSQKKALALAETGIYNYIGSDEE"
     gene            16494..>17182
                     /locus_tag="PLH17_01885"
     CDS             16494..>17182
                     /locus_tag="PLH17_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20566.1"
                     /translation="MEGHQTSMEIYLIGYTLFALALAFVVFVQLVSHRLIFKNNIRIQ
                     QIFAHPLLTYSIRRIGSALISIMLAITATFFLIRIKSEGFMICKQAIGTWDKLGESLR
                     EMQCVALKARLGITENWFVDLFSYYYKILPFPKTVCQVTDVGTIVGTDTIYTISNQNC
                     RNFILDLGTVFFLGGGNNGKFVLDLISEKMVISFRIGIIAVIVELLLGYPMGIMMAKN
                     KDGIFDKIGKT"
     assembly_gap    17183..17282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17458..18450
                     /locus_tag="PLH17_01890"
     CDS             17458..18450
                     /locus_tag="PLH17_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20567.1"
                     /translation="MANNSVRNIDSVIYHNIEDLFTVIGASEESIEKLSTAPYSYWRE
                     TFKQLIKKPLAILSIIVILLIVFFTIFGPMMKSYRVVSNSEGLADIFTPNQSWSADHW
                     FGTGGNKMNFYKGLDLWTVVWVGARLSLILGTVVALIDTFLGILIGSLWGYFRRLDPI
                     MIEIRNFVNNVPSLLLYFLLMAFLTPDFWTIVFLLTMFGWIGLAGFIRNQIIIIRNRD
                     YNIASVTLGSSPKAMITHNLLPYLISVIVTVVSTAIPAAVSSEVGLAFFGLSFSPAAG
                     DVTLGQVLTDATRDEWMTYPYLLIAPMIVLVPLTVTFFYIGLALADATDPKNHR"
     gene            18463..19533
                     /locus_tag="PLH17_01895"
     CDS             18463..19533
                     /locus_tag="PLH17_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20568.1"
                     /translation="MQTKEKVLEVRDLSVGFVSHGKKVNVIRNVTMDVYQGETLAIVG
                     ESGSGKSVFTKTFTGMLDANGYISSGSILFHGEDLTKKTKHEQWKGIRGKRISTIFQD
                     PMTSLNPLLTIGYQIAEVLMLHHGMGMKEAKEEAIKLLEKVRIPDPRGRMNDYPFQYS
                     GGMRQRVVIAIALACRPEILICDEPTTALDVTVQSQILELIKELQREYKWTTIFITHD
                     LGVVANVADRIAVMYGGQIVEYGTAEDIFYTPQHPYTWALLSSLPQLGTKNEPLTYIK
                     GNPPSFVNEIIGDAFAIRNDYAMRIDHVQDPPMFKISNTHYAKTWLLDPRAPKVEKPK
                     IIQNLRERMKQAAVELKREVGE"
     gene            19536..20576
                     /locus_tag="PLH17_01900"
     CDS             19536..20576
                     /locus_tag="PLH17_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20569.1"
                     /translation="MKNLFKSIQKAVIGDHSHEKAGLLDVIPTIENGYDPIVSFKNVQ
                     IGYKRGNVERVIIQGLNLDIMRGEIFGLVGESGSGKTTVGRSLIRVTNVSKGEILYNG
                     VRISGKISRTLSREVKKKIQMIFQDPSASLNDRANVDYIVSEGLRAFHLYEDETDRLE
                     KVEEMMRRVGLRPEHLKRYPHEFSGGQRQRIGIARSMIIKPELIVADEPISALDVSIR
                     AQVLNLIKEFQKESSLTVLFIAHDLSVVKYISDRIGVIYNGKIVELASAERLFEVPLH
                     PYTKSLLSAVPIPDPRVEKVKKVVVYHPQMHQYTHDDEPQLYEVEPGHFVRANSAELA
                     AYKEIVRQGGNK"
     gene            20576..>22539
                     /locus_tag="PLH17_01905"
     CDS             20576..>22539
                     /locus_tag="PLH17_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOR20570.1"
                     /translation="MDGPEFAVIPVEVKQKSKRRLVITWVSVILGILVLLGGAYSSLI
                     YFVLLDYVTMPYITFSYRSDLADESQITVTIDRVYSDSDYPARFRVPRKLMGYPVTAI
                     GPSAFAGLERLEEVIFPETIISIGESAFNNCVNLAKFNVPSALDYIGTDAFNNTAYIA
                     NYPDDAVIIGSILYTYKGIFEDDTAIVKSEDSPAIATHDNYFNLGIFEQIGAGVFANQ
                     PGITYAEFPDSLETISDKLLYNCINLSEVHLGDNVKYIGNEAFYGASSLVSMEWSNQI
                     ESIGDYAFKETNFAGAVVLGTDLKYIGVGAFQNSHAMTSITIPSGLSVLNNYVFDGCE
                     NLAEIILSADEYTPNSRIYDIGIAAFRGTAISEFTIPFSVRTIKDSTFEDTPNLTTVY
                     AYNNTTGTTRTEYTLDEETGELVENQVSQGLQKITIKAFNNSVSFKELVLVDKDNAIE
                     SPLNRVTLPSTMQQLGEANTDSFIFSNTAVEVLDLTSAIRFIAPSLAKDASQLREVII
                     DGGAQSILIGAIYREAFMNCVALESFVVPDTVRVVNSSVFEGCIALESVTLPTNASYI
                     VLDTNLFKGCVSLTAVIIPASIQAIKDRAFENCTSLTAITIPSSVVAMAANVFNGCDN
                     SLQITVMTRNNNTANWNSQWLGTSGLTVEA"
     assembly_gap    22540..22639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22640..23916)
                     /locus_tag="PLH17_01910"
     CDS             complement(<22640..23916)
                     /locus_tag="PLH17_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOR20571.1"
                     /translation="MHETTRTNVIVNLTRTIVLTILSFITFPFVCRVLGDETLGLYTW
                     ANTFVYYFLILARISIPNFAIRECVRVRDDKKALSKKAQEFFILQSITTLLSFALMLV
                     FVFSVPELFTNREIIFILSINFLTGVFSFEWIYIALEKHFYMAMRSIVILTISAICIF
                     VFVKYPEDIYLYAFFTVGVTVLTVLSNLFYLPSFISFKKEGSYDFKSYIKPLLVLFSI
                     SVVLSLYNQSDTFILGFLNADKAEVGSYSVGLKGVDVIIAITTSLSVVFMPRASYYYQ
                     LEDKSFFHKLTKYSLNITLFIAIPAIASMTTLSEPITRLISGNYLGAGGDFQESWTIL
                     TILASLMVTYSIGDIIYTQVLIPMKKEKIYLYTILIGAVLDIVLSIILGLFVFKAHPA
                     IGVALATAVTDAFIIVFLLIKTWRWTGHAVFNI"
     gene            24024..25217
                     /locus_tag="PLH17_01915"
     CDS             24024..25217
                     /locus_tag="PLH17_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR20572.1"
                     /translation="MKILVVCQFYYPERFTVSDICAELVSLGHDVSVVTGKPNYCYNE
                     IIPAYRKVKYEIIDGVKVHRVNLYPRKQSRLSIIRNYLSFHRNAKAFMRRFNEQFDVV
                     LSVSLSPVISIAPAVLYAKKHKVKHVLHCLDLWPESTVVTGAVKKDSPMYKMLFRWSR
                     NLYLACDKIMVSSPSFADYFKEVLHIIDKPFPYVPQPSLGVHENLPPATFKKKYNFVY
                     VGNIGTLQLIGEIAEAGKIIGTRGDVEVHLAGMGLQSENLKKYIADNNLKDIVTYYGP
                     LPLEKAVTLYANADALIVPLKERGTVGKTIPNKLVQYLKYARPIIGSLSGDGRDVLVK
                     TKGAVLADQNAESIAKAMETIIDLPADKKEEMGRQNKKYYEEHFEIRRVAQLIERELL
                     DTKNS"
     gene            complement(25175..26344)
                     /locus_tag="PLH17_01920"
     CDS             complement(25175..26344)
                     /locus_tag="PLH17_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR20573.1"
                     /translation="MKILYVSGAMKDGDFNMYVKNAKYHINPSNQNFHYRFLKALATQ
                     TAVTALTLRPFAKGLFDFQELKSEEAMDGNITFKYLADKAGRVYKLWHRNRSLTKCLS
                     DEIKTFGKDAIILVDAMKFVLAKATLKAAKKNGISVFAIVTDNPHLLSNESRFYVRAI
                     KSLYQKYDGFITLSSGLNELANKKNKPSYVFSGFAEALPELTKPETKPYFFFGGALYE
                     RYGVLNMIEAFRQIKTDRELLIAGHGPLIARIQEITKEDTRIHYLGMLSREEVHKYEQ
                     HADLNINPRLFDENLDKYSVPSKVLEYLASGAPLLSTLHTTLRDEFCGEAIWVKDGSV
                     DELRKAMNLFLNVQNDDMKKKALLAKEKVLAKYGLENQGQLIYEFLVSNSSRSIN"
     gene            complement(26341..27444)
                     /locus_tag="PLH17_01925"
     CDS             complement(26341..27444)
                     /locus_tag="PLH17_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR20574.1"
                     /translation="MKVLFVFNHQAPYKVRLFNELSKDIEVTALFERLKDSDRDPRFY
                     QGEEIKFKTVYIKGLNLGHDNHLSRGVVRHLKNNQYDIIIMNGWHTFSEMAALNYLKK
                     NRKSYVFYINGGIIRKRESSVLRRIKTHYISGADHYFSPDENSNKYLVHYGANANHII
                     NYPYSTLYEREILPKPLDSQEKAALRTILNLTGAKIFVSVGQFIPRKNYERLLNYWKS
                     QPADHRLILIGGGPEKNKYRRLIKELELTNVQILDFLPREKVFQYFRAADVYLFPSKE
                     DIYGHVVNEAMSQGLPVISNQNVNSAHHLIKNGENGYVIDFNDDGKINMAIKDVLNNP
                     EMANAALKTAGQNTIELMAKVHLDAFKDWMERR"
BASE COUNT         7273 a         6635 c         6116 g         7211 t
ORIGIN      
        1 aaatgttatc gttgtcaatt ttattaaaat tatagagacc gggagcaatt cgtgaatgcg
       61 gaaggagtgc aaatgccgtg gaattgatat aataattaca tcatgaacac atttaagaac
      121 cttgagctca tcgataaaga gagaatcaac cgcttggtca gggattcttt ttattattca
      181 gcctattcat cgagcgaact cgtttttgaa aatatgttcg tctggaattt taaccgtcag
      241 atcgaaatat tatggcttga tgaggactta gccctcgtgc gaagcttcga aaaagataat
      301 cgctggattt tctttccacc catctgccgg acacccgata aattcaagca aggtttaacc
      361 catattaaaa ataactatcc cagcgcttta gttggcggat tatctaatga tatgctggca
      421 attgcctcaa ttgaaggggc actgtatctc tatgacgact actattcgga atatatttat
      481 gatccggtcg aattggcaga gatgataggg ggcaaatttt ctcgcaagcg aaatcttatt
      541 gcccaattca agaaaaaata ctcatatgca tttgccccgt atactgacga atatctacaa
      601 gccgtcaaag actttcttta tcgctacgaa caagagggcg gagccggaga agacttcgac
      661 gctattttgt atgctttaaa taatcgcgaa aagattgatt tgttctgcga tttattactg
      721 gttgaaggct tggtcgtcgg tttgagcatc gggaccatca gcgtctttaa tcacgccgtc
      781 atcctgtttg aaaaaaacga tttcaactat gttggcagcg gtgccgttct cgtgcagctg
      841 acgagtgacg cccactaccg caattgccgt gttttgacga ggcaggaaga cttggggttg
      901 ccccagctac gaaaggcaaa actcgctttt aatcctatcg aaaaagaacg caagtattcg
      961 tgcttttttg ataacgccac tattcagctc tatcatttgt atttgtcgtc ttttgaagat
     1021 tcacgcgact atgtcgactt cttcttttta cattattatc atcccgagcg cgcttttggc
     1081 gtccagcgcg acggaacaat tgtttccgct ctccacatca tcatgaaaca aatgatttac
     1141 aataataaaa ttattgatat gccatttatc gtcgctgcca gcaccgatca aaagtaccgc
     1201 cggcaagggc tgatgcgcga ggtgatggcg aaaacttttc aagctctgat cgccgagggt
     1261 cagaccatcg tttcgcttta tcctgtcaat ccggattttt atcgcgacta cggatttgtg
     1321 cagtatacct ttacccgcga catcaattca tatgctaaat cttttgaatg cggcctcgaa
     1381 caaaccagtg atgctgccct tttggctcgc atgtacgata aatgcatcag cgaatacgaa
     1441 ggttatgtcg tccgcgatga acaatattat acgcgctaca tgaattctct atggcaggac
     1501 ggttatgttt ttgagctgat taaaaaagcg ggcgaagtcg tcggatatct cgtccgcaaa
     1561 gatgaagata tcagcgaaat tcttttgtgt ggcgaagaaa aaccgatgca caaagacctc
     1621 gacacaacgg atacttttat gccacatccc gatggtgata taccctcgaa tatgattcgc
     1681 gttctcaatg tcgaaaagct tttgctcagc atgtcttttg accgggatac ttcttttgat
     1741 ctccgcctca agatcactga tatttttgta aatacaaaca atctcgttct cgacttgtcg
     1801 gtgcagagcg gcagactcgt tttgtcgcgc tgcgataact acgattttga agtgagtatc
     1861 gaggccctga ccgaggccct atttaccggc cgcggcgatg atcgtctcgc ctttcttttc
     1921 ccccacaaaa aaatggtttg tttcgataaa ttttaatata agcatgatga ttatactgac
     1981 gtttgtcatg tgataaaata tgagtagtgt tcaagacact tatttttaat gaggtaaatt
     2041 atatatgaaa aaaattgtta tcgttggcgg agtggccggc ggtgcgaccg cggcgaccag
     2101 agcccggagg ctgagcgaag aagatgcgat cattgtcttt gaaaaagacg aacacgtctc
     2161 tttcgccaac tgcggtcttc cttactatat cggtggcgtc atcaccgacc gcgagcgcct
     2221 gctcgtcgaa acgccggaaa catttaaaag caaccacaac atcgatgtcc gcactttctc
     2281 ggaagtgatc gccgtcgata ccaaacacaa aaaagtgacc gttcaggaag ttaaaaccgg
     2341 acgcacttat gaagaaacct acgacaagct attgctttcg cccggagccg cgccgatttg
     2401 gcttccgatt cctggcattg aaaaagccca caatgttttc tctatgagaa acttggtgga
     2461 caccgacaaa ctctttaatt ttatcacgaa agaaaaagtt gaatcagccg tcgtcatcgg
     2521 cggtggtttt atcggcgtcg aactcgccga aaatctcgtt gaacgcggca ttaaggcgac
     2581 gattgtcgat ctcgccgatc aaatcctgcc ccctcttgat ttcgagatgg ccaaaatggc
     2641 tcagaacgaa ctcgagacaa acggcgtaaa agtatatcta aaagattcgg tcgcggaaat
     2701 aaaagataat ggtaaaaccg ccgtcttaaa aagcggaata gtcttgaaaa ccgacttgat
     2761 aatcatggct gtcggcgttc ggcccgaatc gcgcctcgcc atctccggag ggctcaaagt
     2821 cggacccaaa ggtcatatcg tcactaatga gcacctgcag accatcgatg cgaaaagcgg
     2881 cgaagtcatc cctgatgtct atgcggccgg cgacgccatc gaagtcaaag acttcatcga
     2941 tggcactccg accgccgttc ccctcgcctg gccggccaac cgccagggtc gtttggtcgc
     3001 cgaccatatg aatggttggg atgtcagatt taacggcagc ctcggcacct caatcgtcaa
     3061 agtattctct ttgaccgccg ccagcactgg caacaatcag aaaaccctgg agcgaaaagg
     3121 cgtcgctttt cgcgcggtga tggttacccg ctccaatcac gccggctatt atcccggcgc
     3181 aagtgacatc gtcattaaac ttttattcag ccctgaaagc ggcaagattc tcggcgctca
     3241 ggccgtcggc caagacggca ccgataagcg cattgacgtc atcgccacag ctatcaaagg
     3301 cggtttgacc atctttgatt tgccggatct tgaactcgct tatgctccgc cctttggctc
     3361 ttcaaaggat ccggtcaata tcgtcggtta tgtcggcagc aacatgatgc agaaagaatt
     3421 cggcgtcgtt aatcaaaacg aactcgacga atttcgtaaa aaaaaagcac tcttgattga
     3481 tgcccgtact cctttagagt acagcatcgg tcacattcag gattcggtca acattcccta
     3541 ctatggagtg cgccacaatc ttgataaatt acccaaagaa aaagacgctc cgatcgtcgt
     3601 ctactgcaat gtcggtcaca cggcttacct ctttatcaaa gttctcgtca accacggcta
     3661 tacaaacgtc cataacctcc tcggcggcta taagatttac aaagctctcc atcagaaaaa
     3721 tcacgacgaa gaactgcata atcccgaaca ccgcgtgatc gaagaaaaaa cggcggtgaa
     3781 cagcactgat taccgcgagg atgaaataaa aatcttcatc gatgcctgcg gcctccaatg
     3841 cccgggcccg atcatgcaga cttacaaagc cgtcgaaaaa ttaaaagacg gcgaaatcgt
     3901 caagatcatc gccaccgaca gcggctattt cagcgatgtc gaaaagtgga cggtcgcgac
     3961 cggcaacacc ttactgaaaa aggaagcgac gaaagaaggc tatacggccg tcattcgcaa
     4021 aggctcactc aaaccggtcc aaaaagccgt cgaaaacgaa aatacgacca tcgtcttatt
     4081 tagcggcgag atggacaaag ccatggccgc catgatcatc ggtcaaggca gccgttcaat
     4141 gggcaaaaat gtcactattt tctgcacgtt ctggggtcta aacttgctac gcaaagatca
     4201 gaaagtcaaa gttaaaaagt cctttatcga gaagatgttt ggcgcgatga tgccgcgcgg
     4261 cgcgaaaaaa atgccgattt caaagatgaa cttcggcggc atgggcgcca agatgatgaa
     4321 gtcggtcatg aaaaagaaaa atgtcgattc tctggaaacc ctgatcgccc aggcgaaagc
     4381 ggcgggcgtc aaattcatcg cctgcacgat gagcatggat gtgatgggca ttcacagaga
     4441 agagctcatc gacggcatcg aatacgcggg agtggccact tatcttgcgg aatcggaaaa
     4501 agccggcgtc accctcttta tctaaacgat tattgcttta aaaagccact tgcgacaaat
     4561 aagtggcttt tttgtatccg taaaaggatt gaattgcgcg ccgataatcc ataccattat
     4621 taatagacct tcaaatttat atttgatcgt cgcatcaaat ctatggccat catccatctc
     4681 accgactatg tccgcaaaga aaacgccaag ttaaaactcg gcacttttcg cgatgtcgat
     4741 gctctggtgt tcgcccagct ttcctatctt gattacagcc cctataaaaa cgggattaaa
     4801 ttagaattta cagccgaaaa ggcgaaagaa gccgcccgcc ccagccacat gaaagaggag
     4861 aatgaagacc tcttgcggtg cgcggctaaa agcctccgct atcagcatat taaagtttgc
     4921 aggcatgtcg ccgagacaaa cttgctattc gaactccagt tttcggcaac cacctttctt
     4981 atcagccccc gctttatcgt catcgccttt cgcggcacgg acgacacgat caacggctgg
     5041 aaggaagatt ttaatatggt cttcctcgac gaaattcccg cgcaaacgaa ggcggttaac
     5101 tatgttaaag caatcgcaaa gaaatatcgt tttgcccgaa ttctgatcac cgggcattcc
     5161 aagggcggaa atttggcttt ttatgcggcg atgaaacaga gtaaagccgt caaaaagcgc
     5221 atcgtcagca tctacaatct cgacggtccc gattttcgcg aaaacatcac ggcctccgcc
     5281 gattatattg acttgctacc caaggtcatc aaaatcgttc ccgaagagag catcatcggc
     5341 atgattcttc agaccaacga ctactatcac atcgtcaaat ccgaaggaac cctctttgct
     5401 cagcacaaga tgtataactg gcacatcgac agcgagacga gcgatctcgt tcacgagatg
     5461 tcgctcagca aatcgtcaaa aaagattaag aattccctct atgcctggct ctacagtttt
     5521 aacgtccatg accgccagat tatcatcgac aacttctttg atatgctcca ggcctcggag
     5581 tgcacgaccc tcagcgcttt ctccagcaat ttgtccggca acatcaaggc gatgtacggc
     5641 gagtacaaag agtcggacag cgagactaga aaactcattc gtaaaacctt tatcgaactc
     5701 gctaaatact taatgtccaa ctacttaaca agagaaaaag atctcgacaa aaaagccaag
     5761 aaatttgcat taaaacgcaa aattaaagaa ggtgaataag atgccctcca gcacgattgt
     5821 ccaattgata aaatataccg aatcgctttc caatcgcgag atgatgaaat gggctcttcg
     5881 caatgtccgc gaagtgcttg atgttttaaa catcaacgtc acaaatgatg agcagtaccg
     5941 cctgatggtc atcgaaacga atcgctttct gaccggattt cacaaagtgc cgcacatgcg
     6001 caatcaggcc ttcaagatgc acgaactcgc ccgtcacgaa aacgatcccg tccgcgtcaa
     6061 tttactgagg gcatacggac acatcatcgc gaccgcccac gtcaaagagc atgcattgca
     6121 tgcgaccgaa tatgctaaca aggccttgcg cgccgccaac aagagcgaag aagacatcga
     6181 aaaaatcgtt gacgctcaga acaatgcttt agaatcactg gcccgcttta tcgtttcagt
     6241 tgataggccg atagtcaatt agataagata ttgctgcgcc cctcccctct cgcgcagtaa
     6301 aggaactcta tttatgaaca caaccatcct cctcgcccac ccctggcacg gcagttttaa
     6361 caaagcgatc cttgataccg tcaccggcaa acttgcgaac ttgaacaaat catatcaaat
     6421 tatagatttg aataaagacg ggtttaatcc tgtcatgacc gaaaaggaat tagccctttt
     6481 ctcaagaggc caattctccg atccattagt cggaaagtat caaaagatgc tcgccgacac
     6541 cgacgaattg gtcgtcatct ttccgatctg gtggggcgac agcccggcca tcctcaaggg
     6601 ttttatcgac aaagtcatgc tcaagggttt tgcctatgag caaggtgcat ccggcttaat
     6661 cggaaagctg acccacatca aaaaaggcta tgtcatcacg acttcggaaa caccgacctt
     6721 cgtttacaaa gagcacctcg gcaatccgat tgacagcatc ttcttaaaaa ttatcttgca
     6781 aagcatcggc gttaaaaaca atgtctggat aaacaaagac atggtctttt caggcgggca
     6841 ggaaagccgc gttgcctttc taaaagaaat cgccggccta tttgataaat aaagaaaaaa
     6901 taagggctct attaactcta gcgtgggacg atacaaaaca tcacaccttg gattttacag
     6961 ctccaaaata agtttaagaa gcggagctat gctctgcttt ttcttttctg gacagcgtga
     7021 ctagaaaagc ggttgcgaca ccgagcatag ccccggagag atcgatgccg acatcgtaga
     7081 agttaccgct tcgcccgctg gtaaagagct gaatcagctc cgaggctccg gcgatcatga
     7141 tcatcaagga gatggcaaga ataaccggca accacttctt cttcataatg gataagttga
     7201 tgagcattaa ataggcaaat ataccatcga gcaaaaagat gagaaaatga ccgatgattt
     7261 tccggacaaa aaaggcaaaa gtatcaagtt gactctcatt gagaatgacg ccgaaaaagc
     7321 ggaggatgct atttagaata tcgacgacaa agttgctttg cgaagaggag atctcgccgc
     7381 taaaagcgga gaagacaaga atgacggtcg caaaagcgac gaaaagggac agatagataa
     7441 ttccgggagt ggtgataact ctctttttca gcttggtcat ataagcagta tagcaattat
     7501 taataattac tagaaagcaa aaaaagatga gcgttcatct atctttcaga ctatgacacc
     7561 tgtaatattt aatatggagg acttcacaat ggttttcgta aaatatttaa tactgattgc
     7621 cttgacgttt gtggtctttt tagtctttga tttagtatgg cttattaaaa tatcgccgcg
     7681 tttctataag aaaaatctcg gtcatctgat ggcggagaaa gtcaactatg ccccggcgat
     7741 aatcttctac ttgattttta ttgtcggtat tactttgttt gtcatcggtc ctgcttatgt
     7801 cggtggacgc ggctggtcat acgctctgat ttacggtgtg gtctttggtc tcgtcaccta
     7861 ttcaacttat gatctcacga atttcgcaac gctaaagaag tggcctttaa atgtcacctt
     7921 agcggatatc gcatggggta gttttctttc gaccgcgacg tcgatttccg tctactatct
     7981 cgccgccttg atgggggttt aaataatggc tattctcgga tatgtcctgc cccctgttct
     8041 cgtcatcttg ttttttacaa ttacttattt tgtcgccgaa actaaaaaga attatggcta
     8101 catcgatatc gtctggggag ccagttttgt catcagcgcc tggtcggcgt tagtcatcac
     8161 ctccctcgtc aacatggcgt ggccgagcat tcccgcgatc gtggttgcgg ttgcggtgac
     8221 gatttggggt cttagactct cgacttactt atttaagcgc aactggaaca aaccagaaga
     8281 ctaccgctat gtgacgatgc gcaataatct cgccggcaaa aaacatccca agcttttagc
     8341 ttttataaaa atcttcatgt cgcaagccgg cctttcaatt attatttcga cagtcattct
     8401 cgttactctt aacggcgtcg ttatcgccaa tgattggctc gtctatattt tcgtaattgc
     8461 tggaggcctg ctttggatta tcggtttctt ttttgaaagc gtcggcgatg cccagctccg
     8521 cagcttcatc cgcaaaccag aaaaccgcgg caagattatg gaccggggat tatggggcat
     8581 cacccgccac cccaactact ttggtgaatc cctgatgtgg tttggtttag gcgtcgtcgc
     8641 tctcgccgtc gcctatggtt atatcggctt tgcctcgtat atcatcatca cgagtttgct
     8701 gttgtttgtt tccggcgttc cgctcctgga aaaagccttt aaggatcgcc ccggatttac
     8761 tgaatacgcc gcgcgcgtca gcatctttat tccctggttt cccaagaaga aaaaatagtt
     8821 aggagaattt gttatcatga aaaaagttca catcatcacc gacagcacgt cagacatcag
     8881 ccaacagcaa gccgagaaac tcggcattga tgtcgttccg ctgcatgtca atatcgatgg
     8941 tgtcgattat atcgatggtt tgacaatcag caatcaggat ttctttaaaa agctgagaac
     9001 tgcaaagatt ttgccgaaga catcggcgcc cagtccgggt tcgtttcttg acgtaattaa
     9061 aaaatatccc aaggacgaag agatcttaat catgacgata tcaagtgatg ccagcgcgac
     9121 gtacagttca gccaaattgg ccgttgaaga agcggaacgt gataatatcg ttctatacga
     9181 tacaaggcag gccacgttcg gtttactggc gttggtacat gccgctattc agtttcgcga
     9241 tgtcggcgac aatgccaaga caattgttga aaaagttcag gaaatcagca gacgcattgt
     9301 cgtgcaggcc gcttttgacg atctccgcta tttaaggatg ggcggccgta ttccatttac
     9361 tttggcaatc cttggttcgg tcatcaacat gaagccgatt atcaccatgc aagacggcaa
     9421 gattcactcg gcggccaaaa tcatcggcat gcagcgagcc ttgaatcata tcgtctataa
     9481 tctcgccgat aacaatgtcg attattccct gccgactttt gtcggtcata ccgataatca
     9541 cgagatgttt aaaaggttta ctgatttaat taagattcat aaaccggaat tcccgcttga
     9601 tcgcgaagct tcagtcggct tggcggtcgg tacgcacgcc ggtcccggct gcgtcgccat
     9661 cgtctacttt actaagtaac aatcctgact gaaatataac aaagaacttg ctataatatt
     9721 ttcgtcatgg agatgaattt atgaaacata gtaagcttct cggcctcctc gtcctcttgg
     9781 tgacatatac ggccgccttt tttgttggtc tcattatttt caatttattt aaatcctggg
     9841 tgcccgatgt tttaatgcgc attttaatcg ccgatgtcat cgccactctc tttgtgtgga
     9901 gcgtcggtct cgtcttcaag acggcttccg tctacgaccc ttactggagc gttcagcccc
     9961 tcgtcatcct cctggcgttg atgatttatc gcggcgtctt tgacttgggc gccatcgtcc
    10021 tgctttcgct cgtggctttg tggagccttc gcttaacggc gaacttcatc tatactttca
    10081 gcgacctctc ctatcaggac tggcgttata cgatgctgcg cgaaaagagc gggaaattgt
    10141 actttttcgt caacttgttc ggcatcaatc tctttccgac tctcgtcgtc tacgcctgca
    10201 tttatccgat gctcctctat gtcgaaagag gaggaagcgc gacttttaat cttttatccc
    10261 ttatcggcat catcctggcc gtcatcgcca ttctctttga gacggtcgcc gatgtccaga
    10321 tgcagcagtt tcggagcgct cccgaaaaca aaggcaagat tatccgcgtc ggtttgtgga
    10381 agtacaaccg ccaccccaat tacttcggcg aaatcctgat gtggtgggga atatatgccg
    10441 ttgtgctggc gctaaatcct tccatctggt ttgttttctt tggcgctttg atcaacacct
    10501 taatgtttat gattatttcc atcccgatgg cggagcgccg cctggccggt tacaaagcgg
    10561 gctttaatga atatcgcgat gagacatgga tgctctttcc cctgcccttt gcaaaaggta
    10621 aacgccggga acagtaatac tccttcgcgc ccattcgtat ataattaaaa gaaatagggg
    10681 tagttttatg gatttgaaaa aaagactata tgctctggtt gataaatacg atgcgctgat
    10741 cattttctta gtcatcgaat gcctggccct gaccgctttt tctttagccg acgccaacat
    10801 catcttccgc tacctcggct ttttaatttc tttagtccta attccctttg ctttaatgtt
    10861 cacctcaaag cgcgaatggc tgtcgctcgg gctcttttcg attcccttag tcatcatcgc
    10921 ctttttaacc ggtttttcga cttttacctt atcgatgggg ttcggttcac tcgacaacct
    10981 ttcggtcgtc ctcgggactc tcgctttctt cttcctcggc tttgccgtcc ggcgcgtccg
    11041 gaagtttaaa atcgatatcg ctttactggc gatcggcgcg gcgatggcgc ttttagtctt
    11101 cctcggcctc atctattccc tttatcaata cggaccattc cacgcctcat tatacgaagg
    11161 tctggtttac tattacgacg ggcgcctctt tgtcgtcagc gaagagacca agtggctgct
    11221 cggcttctct tttgtcgaga tttcgattaa ttatgtctca ctcttcgggg cgatgctcag
    11281 cacggcgctt acggccctgc tttatatatc gccaaaaacg gaaccgcgca aattcgcgat
    11341 tgtggccgtc atcggcctca tcggattatt gtcaatcgcg ttaattccca atatcgaggc
    11401 tttaatctta ttgattcccg ttttcctgtt tgccctcggc tatcgcttta ttcgctctcg
    11461 cgaaaaagtc ggcatcatcg tgcaatatgc cttctttgct cttctgggta tcgccggcat
    11521 cgcgcttcta gtcgttattc tgaataatgc gggtgtttcg tttatacgaa atgcgctggc
    11581 cgggaatgct tttctaaacc gcctttttaa tacgaatcgc atcatgtcac cagtcaatgc
    11641 agtcttggct ccgatgttta ccgcgggtgg attttttggc tttccgatca cttatatcga
    11701 cggcttggtg gccaacaccg gcgccttcga atttgaaatc gttaaggagg gtggtttgct
    11761 ggcttttctc gctatcatcg cttttctcct tttagtgatt ttctcgttag cgcgatactc
    11821 agcgaaaagc cccgacaaag ggcatatcaa aattgtcatt ttatcgcttt taatcgctgc
    11881 attactaaat ggcagcttat tatgggatac cttcccgttg atatatgaga gcgattatta
    11941 tttttcgttc ttcagaagcg ccccttatct gattgttttg ttcttgatcg gctatgctta
    12001 ttacccgctt tccggtaagc cgttaattga agagccagaa gagattgtgc tggtctccga
    12061 gacgacaaac gccgatcacc tcgctaatag cgacgaggag gaaatcaaac tatgagaaag
    12121 aaagctgctt ttgcccttct atttactttt gcattcgttt taaccggctg catgaacgat
    12181 gtcgccgagc tttacgaagg ccacgcctat gataccggcg tcttttctga aaactactat
    12241 tcgacatgga atgacagcct caaaaatagc gtcgcgccgg tcgccaagac atatgaggtg
    12301 acggccgcga actcctttca gatcgaacag gatagcgatt ttgctcannn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnncac tttgccttaa tgctttatga agtgctccta
    12481 cctaaatcaa cctggaatta attaaatctt tttctttagt tttggatgat gaaccaaata
    12541 aggttcgata tttagccatg cgcccatctc gaaatcgcat cgatcgtcat cgaggatgag
    12601 agtgcccttc gcgggcgtaa aggtcagttc gccgaaatag attttgccgt cgatgttata
    12661 taagtcgact cgcacaaacg gaaagggctt ggcgagatct tcagccattt tgaccatctc
    12721 tatccaattt tcgggaatcg gaatcagttc gtcgcgcttt ttccagccgt taaactgcga
    12781 gccgtcaaac ggcttccagt cgatgtaata gttgttgtag cggatgtcgg cgccgcggcc
    12841 ggtgacgacg tacatgctcc ttgcttggcc gttaaagaca tggattttgt actcgaccgg
    12901 caaggagtcg acttcaccga tatatttctc aatgataatc tgcggtttga tcggcgcgta
    12961 gtggagctca agcgtctttt gtccatagtt ggttttgagc catttttcga agcgtttttt
    13021 aagaggaatc aagtcgagat tagccttgtc gcgaacgatg acattgtaag cgcaggcatg
    13081 ggtggctttc atgacaaaag cagagggcaa atgcgcgaaa tcaatctcat taaaatgatt
    13141 ataaaggccg tggattggaa tgagggacgc gccgtaaccg cggtcttcca catactcgcg
    13201 gactcccgct cttcccgcgc atttgatgac taggtcatca tgcgggtaga cgaacaggcg
    13261 cagatactgg agtttttcgg tgtagcggag gggcttttcg aggttgagtt tatggtgggt
    13321 gatgtactta tattgttgct tgacaaagaa gacggggaaa agctttttaa tgatgccgcg
    13381 aaagggggcg tagagagccc tttttaactt gttttcttga aggtttggat tagccataat
    13441 ctttggtaaa aatagtatat gctcaaacat tcatttttac aaacaggaga aatgtgatag
    13501 tatacaatac tacctgagaa caaacatgaa aaaagcattg cgcagttggt taacatatgt
    13561 cgtcgccgga gccctttttc tcgtggccgc cggcatcgcc gtcatccccg atatcgtcga
    13621 taactatgtg gcgacgatgg aagagtggta cgcccattat atcttccttt ttatcggcat
    13681 cgccttgatc tttgtcggtt tcgtctggca ggacctttta aaggctcagc atcgtcgtaa
    13741 gacgaagaac tgggacgggc ccttatccga ggatgtcacc aatggggcct gggccgtctc
    13801 cctgcccttc tatctggctg ccgtcttgtc aatcatcggc ggaattatct tcacttttgt
    13861 ggccatttaa aggcaaaaaa acttgtaaac aaagcatttt tttgagaata accacctaaa
    13921 aggtggtttt ttattcacat tccatgtaat cgcttacagg ctaaaaaaat cgttgataca
    13981 tattttgata agtgttaata taaaacacaa tctaaaagat tgaaaggaat aggaggacaa
    14041 aaatatgact tttcctaaaa agacagcttt gctattagcc gctcttgtgt tagtcgttcc
    14101 tctttcgtca tgtgacggtg gtggttcgga caaattttat gtcacgggcc catactttac
    14161 cggttacgaa gctaaagatg tgtacaacgc atatttgggc tctagcatca gcaccctcaa
    14221 cagcgccaaa agccaaagcg cgaccgatgt tcgccactta gcgaactttg ttgatggctt
    14281 ggtcatgaac aacgaattcg gtattctcga acgtcaactc gcttcgtcgg caagcgttaa
    14341 cacggaatac acgcagttcg atttcacaat caagcagaac atcccgtggt ttacttatga
    14401 cggcgctcag tatgtcgcca agaacaccga gggacaagat gttccccaat tcgttaaggc
    14461 tgacgacttt gtcaacaccg ccgaaattat tctcaactac gccaatgaat ccgaaatcgc
    14521 ctacatggtg acgatgttta tcgacggcgg atgggaatac tactgctaca ccgagatgct
    14581 caagttaatc aaggatcgca acaccaacta tgccggcatt aactggatga cgctgaaaag
    14641 cgcctcgaat gatgccaaag ccacggctct catgactctc atcgcttatg aatcaggcat
    14701 ggatccaatc gatgtgcccc tcattttggg tgaagattta gaaagcatcg ccaatttctc
    14761 acgcgtcggc gttaaggcga ctagcgaata tggcattcag tatcgtttga atcaaccagc
    14821 ccgcttcttc ccgacccttc tcacctacgc cccattctat ccgattaacc gcgctttctt
    14881 ctcccagcac aagtttgccg gatttggaac gagcaaagaa aaattgctgt ataacggacc
    14941 ttatctctgc tccgaatgga gtgacaccaa agtccaatac acccgcaatg agaactatta
    15001 caacgtcgaa gatgttcaca ttaaaacggt caactacatg gttgcccccg acacaatctc
    15061 ttacgccgaa atgcgcgaag actttgaaaa cggcgtcatc gacggattta ccatcgccaa
    15121 agaagactca gtcggctggg cccaatacat taccggcgga actcctgagg ctccgggtag
    15181 cggcactgtc caaaacccct atagcccgtt agtcaacagc cgcgaactca atacgatcga
    15241 ctacacctgg cacttcaccc tcaacgtgga gagagaaacc gatgtcagcc tccagccgaa
    15301 taccattttc ggcggtgagt ctccggatta ccccgccagc gcgattgcca acaccaacaa
    15361 agctatgaaa atcaaagaag ttcgccgcct gattttagac ggcatcgatt tcgagcaata
    15421 caatcagacg aacaccaata ccgaagatat tcaccaatat cagctgaata ccttgacgcc
    15481 tcccggatac gttttcgatg aaaacgacaa ggattacctt gaatattatt acgaagagta
    15541 tgccgagaat aaaggacttg aaaacggcgc agccgccaaa gccttaatcg gcccgcaaaa
    15601 gatcgaaggc gtcaactact cggacgccga agtattaaca atgcgcgccg aagccgcgga
    15661 tgctgttgcc caatacaacg cgctcaatcc gaccgatccg attaccacgc caatcatcgt
    15721 tgaaaacgct ggtttagccg gctatgctgg aacgagctat atcactgctg aagcgcgttg
    15781 gattaatgac tttaatgata gggttaacag cggtcttgcc gaaggcaatg aattcctgac
    15841 aatcgtcaac aacctcaaag ccaccacctc ggataaagcc tcgaataacg cttatgccgc
    15901 ttactatact ctcaactcat ggggctggat tggcgactat gctgacccgc taacttacct
    15961 ccacacttac gtcaccaatg gcgatatgtc attattcacc ggcaccaaag ccgcccgtga
    16021 caactattat ctcaatgcca gcgatgaact cgtctatgaa gaagacggat tactcgaaga
    16081 ctatgacgat ttagttgatg atgccaaagc gattacgagc agcaacaatc tgcgctacga
    16141 cgcctttgcc aaagcggagt acgaattaat taatgatgtt tacatcatca aaccgcactc
    16201 catgaacagc cagggctggg ccgtctccgt ctcccgcgcc atcggctatg aaaacccgac
    16261 cgcctcttat ggtctcgccg attaccgcct gaccggtatg tgggttctcg ttgaacctcc
    16321 gaccggagcc gaaagacaag tggctcgcga tttacaaagc cagaagaaag ctcttgctct
    16381 cgccgaaacc ggtatttata actacattgg ttctgacgaa gaatagttag ttaagtttaa
    16441 cgcaaatcag gatcgatgaa aattggtcct gatttgttgt tttattatca tgcgtggaag
    16501 gacatcaaac atcaatggaa atatatttga taggctatac cctgtttgcg ctggcactcg
    16561 ccttcgtggt ttttgttcag ctcgtcagcc atcgccttat ttttaaaaat aacatacgaa
    16621 ttcaacaaat ctttgcccat cctctgctca catactcaat tcgaagaatt ggtagcgccc
    16681 ttatctccat catgctggcg attacagcga ccttcttttt aattcgtatc aaaagtgaag
    16741 gattcatgat ttgtaagcag gccatcggca catgggataa actcggagaa agcctccgtg
    16801 aaatgcaatg tgtcgctctc aaggctcgtc tcggtattac cgagaactgg tttgttgatt
    16861 tattttcgta ttactacaag atcttgccgt ttcccaagac cgtctgtcag gtaacagatg
    16921 tcggcactat cgtcggcacc gacaccatct acacgatttc caatcagaac tgccgcaact
    16981 tcattcttga tcttggcacc gtcttctttt taggcggcgg caacaacggt aagttcgttc
    17041 ttgatctcat ctccgaaaag atggtcatct catttcgcat cggcatcatc gccgtcatcg
    17101 tcgaattgct cctcggctat ccgatgggca tcatgatggc caagaataaa gatggcatct
    17161 ttgataaaat tggcaagacc tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nngatggtat cggcggactc ctcgtcaaat cggaatcgac aaatgactat ttcgttttgc
    17341 agggtatcat cgtcatctcg gccttgattt ccattatttc gtatcttgcc ggtgatatcg
    17401 tcaccgcggt cgttgacccg cgcgtcagtt tttcaaaaga gtaaggaggg ataagacatg
    17461 gctaacaatt ctgtcaggaa tatcgattcc gtcatctacc acaacatcga agacctcttc
    17521 accgtcattg gcgcgagcga ggagagcatc gaaaaattat cgaccgctcc gtattcatac
    17581 tggcgggaaa cttttaaaca attaatcaaa aaaccactcg ccattttaag tatcatcgtc
    17641 atcttgctaa ttgtcttctt caccattttc ggtccgatga tgaaatcgta ccgcgtcgtc
    17701 agcaattccg aagggctggc tgacatcttc actcctaatc aaagctggag cgccgaccac
    17761 tggttcggca ccggcggcaa taagatgaac ttctataaag gtctcgacct ctggaccgtc
    17821 gtctgggtcg gagcgaggct gtcgctgatc ctcggcaccg tcgtggctct catcgacact
    17881 ttcctcggca tcctcatcgg ttctctgtgg ggttatttcc gccgcctcga tccaatcatg
    17941 atcgaaattc gcaacttcgt taataatgtt ccatcgctgc ttttatactt cttgttaatg
    18001 gcctttttga cacccgactt ctggacgatc gtcttcttac tgacgatgtt tggctggatc
    18061 ggcttagccg gtttcatccg caatcagatc atcatcatcc gcaaccgcga ttacaatatc
    18121 gcctccgtaa cccttggctc ttcgccaaag gcgatgatta cccataactt gctcccgtat
    18181 ctaatttcag ttatcgtcac cgtcgtttca accgccattc cggccgcggt cagcagcgaa
    18241 gtcggcctcg ccttcttcgg cctttccttc agtcccgccg cgggcgatgt tacgctcggt
    18301 caagtattaa ctgatgcaac gcgcgatgaa tggatgacct atccatattt gctcatcgct
    18361 ccgatgatcg ttctcgtgcc gttaaccgtc accttctttt atatcggctt agcgcttgct
    18421 gatgccaccg acccgaagaa tcacaggtag aaaggataaa atatgcaaac caaagaaaaa
    18481 gtattagaag ttcgcgattt atccgtcggc tttgtgtctc acggcaaaaa agttaatgtc
    18541 atccgcaatg tcaccatgga tgtctatcag ggcgaaaccc tcgccatcgt cggcgaatcc
    18601 ggttcgggaa aatcagtctt tacaaaaacc ttcaccggca tgctcgacgc caacggatat
    18661 atttccagcg gctcgatatt gttccacggc gaagatctca ccaagaagac gaaacatgag
    18721 cagtggaaag gcattcgcgg caagcgcatc tcaaccatct tccaagatcc gatgacctcg
    18781 cttaatccgc tgttgacaat cggctatcaa atcgccgaag tgctgatgct tcaccatggc
    18841 atgggtatga aagaggctaa agaggaagca atcaaacttc tcgaaaaggt ccgcattccc
    18901 gacccccgtg gccgaatgaa cgattatccc ttccagtatt ccggcggcat gagacagcgc
    18961 gtcgtcatcg ccatcgcttt ggcgtgccgt cccgaaatcc tcatctgcga cgaaccgacg
    19021 accgcccttg acgtgaccgt ccaatcgcaa attctggaac tcattaaaga attgcaaaga
    19081 gaatataaat ggacgacgat ttttattacg cacgacctcg gcgtcgtcgc taatgtcgcc
    19141 gaccgcatcg ccgtcatgta cggcggacaa atcgtcgaat acggcacggc cgaagacatc
    19201 ttctacacgc cgcaacatcc atatacctgg gcccttcttt catcgcttcc gcagttagga
    19261 acaaaaaacg aaccgctcac ctatatcaaa ggcaatccgc cctcatttgt gaacgagatt
    19321 atcggcgatg ctttcgccat ccgcaacgac tatgcgatgc gcattgatca cgtccaggat
    19381 ccgccgatgt ttaaaatatc taatacgcat tacgccaaga catggctcct tgatccgcgc
    19441 gcaccgaaag tcgaaaaacc gaagattatt caaaatcttc gcgagcgcat gaaacaagcc
    19501 gctgtcgagc ttaaaaggga agtgggtgaa taaacatgaa aaatttattt aaatccattc
    19561 aaaaagccgt gattggcgac cattcccacg aaaaagccgg gcttttagat gttattccaa
    19621 ccattgaaaa tggctatgat cccatcgtct cgtttaaaaa tgttcagatt ggctacaaac
    19681 gcggaaatgt cgaaagagtt attattcaag gattaaacct cgatatcatg cgcggcgaga
    19741 tctttggtct cgtcggcgag tccggatccg gcaaaacgac ggtcggaaga tcgcttatcc
    19801 gcgtcacgaa tgtcagcaaa ggcgaaatcc tctataacgg cgttcgcatc tccggcaaaa
    19861 tcagccgcac tctctcgcgg gaagtaaaga aaaaaattca gatgattttt caggatccga
    19921 gcgcctcgct taacgaccgc gccaatgtcg actatatcgt ctccgaagga ttacgggctt
    19981 tccatcttta tgaagatgag acggaccgct tagaaaaagt cgaagagatg atgcgccgcg
    20041 tcggcctccg tcccgagcac ttaaagcgct acccgcatga gttcagcggt ggccaaagac
    20101 aaagaattgg catcgcccgt tcgatgatta tcaaacccga actcatcgtc gctgatgaac
    20161 cgatttcggc tctcgatgtc tcgattcgcg ctcaagtctt aaacttaatt aaagagttcc
    20221 agaaagaatc gagcttgacc gttttgttta tcgcccatga tttatcggtc gtcaaatata
    20281 tctccgaccg catcggcgtt atctacaacg gaaaaatcgt tgaactggcc tcggccgaaa
    20341 ggttattcga agtgccttta cacccttaca ccaaatcgct cttgtcagct gttccgattc
    20401 ctgatccgcg cgttgaaaaa gtcaaaaaag tcgtcgtcta tcatccgcag atgcaccaat
    20461 acacgcacga tgacgaaccg cagctctacg aagtggaacc aggacatttt gtccgcgcca
    20521 acagcgccga actagccgca tataaagaaa ttgtcagaca aggagggaac aaataatgga
    20581 tggaccagaa tttgccgtca taccggttga agtaaaacaa aagtcaaaac gccgtctcgt
    20641 catcacatgg gtttctgtta ttttaggtat tctcgtcttg ctgggcggag cctactcctc
    20701 gctcatctat ttcgtgcttt tagattatgt tacaatgcct tacatcactt tctcatatcg
    20761 ctccgatctg gctgatgaaa gccagattac ggtgacgatt gaccgcgtct attccgacag
    20821 cgactatccc gcccgcttcc gcgtgccccg caagttgatg ggctatccgg tcacggcgat
    20881 cggcccgtcg gcctttgccg gtctggaacg gcttgaagaa gtcatcttcc ccgagacaat
    20941 tatctcaatc ggcgagagcg cttttaacaa ctgtgtcaac ctcgccaaat tcaatgttcc
    21001 gagcgctctt gattatatcg gtacggacgc ctttaataac acggcctata tcgctaatta
    21061 tcccgatgat gccgtaatta ttggttcgat tctttataca tacaaaggca tttttgaaga
    21121 tgataccgcg attgtcaaaa gcgaagattc gccggccatc gccactcacg ataattattt
    21181 taacctcggc attttcgaac agatcggcgc gggtgttttc gccaatcagc ccggcatcac
    21241 atatgccgaa ttccccgata gccttgaaac gatttccgat aagttactct acaattgcat
    21301 caacttgagc gaagtccacc tcggcgacaa cgtaaaatac atcggcaacg aagctttcta
    21361 tggcgcctcg tcattagtga gcatggaatg gtcgaatcag attgaatcga tcggcgacta
    21421 cgcttttaaa gaaacgaatt ttgccggcgc ggtcgtcttg ggcactgatt tgaaatatat
    21481 cggtgtcggc gcttttcaaa actcgcatgc gatgaccagc atcacgattc catccggcct
    21541 cagcgtctta aataactatg tctttgatgg ctgtgaaaat ctcgccgaga ttatcttatc
    21601 cgcggacgaa tataccccga atagtcgcat ctatgacatc ggcatcgccg ccttccgcgg
    21661 caccgccatc agcgaattca ccattccctt cagcgttcgg acgattaagg acagcacttt
    21721 tgaagatact ccgaatctga cgacggtcta tgcctataac aacacgaccg gcacgacgag
    21781 aaccgaatat accctcgatg aagaaacggg cgagttggtt gaaaaccaag tctcgcaagg
    21841 cttacaaaag attaccatca aagccttcaa taattccgtc agctttaaag aactcgtctt
    21901 ggtcgacaaa gacaacgcca tcgaaagccc gctcaaccgc gtgacgcttc cgagcacgat
    21961 gcagcaactc ggcgaagcta ataccgattc gtttatcttt tcgaacacgg ccgtcgaagt
    22021 cctcgatttg acaagcgcga ttcgctttat cgctccctcg ctggctaaag atgcgagcca
    22081 gcttcgcgaa gtcatcattg atggaggagc ccaatcgatt ctgatcggcg cgatttatcg
    22141 cgaagcgttt atgaactgtg tcgccttgga atcgtttgtt gtccccgaca ccgtccgcgt
    22201 cgttaattct tccgtcttcg aaggctgtat cgcccttgag tccgtaactc ttcccacgaa
    22261 tgcgagctat atcgttttgg acacgaactt gtttaaaggt tgtgtctcct taacagcggt
    22321 tattattccc gcctcgattc aagctattaa ggaccgcgct tttgaaaact gcacgagcct
    22381 taccgcgatt acgattccgt caagcgtcgt ggcgatggcg gccaacgtct ttaacggctg
    22441 cgataattcc cttcaaatca cggtcatgac ccgtaacaac aacaccgcga actggaattc
    22501 ccagtggctt ggcacctcgg gattaaccgt cgaagctaan nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnt tgatattaaa gacggcatgc ccggtccacc tccatgtctt
    22681 gatgaggaga aagacgatga tgaaggcatc ggtgacggcg gtggcgaggg ccacgccgat
    22741 cgcgggatga gccttgaaaa caaagagtcc taagataata gaaaggacaa tgtcgaggac
    22801 ggcgccgatg agaatggtgt agagataaat cttttctttc ttcatcggaa tgagcacttg
    22861 cgtgtagatg atatcgccga ttgaatatgt gaccatcaag gaggcgagga tggtaagaat
    22921 cgtccacgac tcttggaagt cgccgccggc tcctagatag ttgccggata tcaggcgcgt
    22981 gatcggctcg ctcaaagtcg tcatcgaggc gatggcggga atggcgatga aaagagtgat
    23041 gttaagcgag tacttcgtca gtttatgaaa gaacgatttg tcttcgagtt gatagtaata
    23101 gctcgcccgc ggcatgaaga cgacgcttaa agaagtggtg atggcgatga taacatcgac
    23161 gccctttaac ccgaccgaat aacttccgac ttccgccttg tctgcgttta aaaagccgag
    23221 gataaacgta tcgctttgat tatataaaga taggacgaca gaaatgctaa ataagacgag
    23281 caagggcttg atgtatgatt taaaatcata agatccctct tttttaaacg agataaagct
    23341 tggtaaatag aagagattgg ataagacggt gaggacggtc acgccgacgg tgaagaaagc
    23401 gtaaagatat atatcttcgg ggtatttgac gaagacgaag atgcagatgg cgctgatcgt
    23461 taaaataact atcgaacgca tcgccatgta gaagtgcttt tcaagcgcga tatatatcca
    23521 ttcaaaggag aagacgccgg ttaagaaatt gatgctgagg ataaagatga tttcgcggtt
    23581 agtgaatagc tcgggaaccg aaaagacaaa gacgagcatc aacgcaaatg aaaggagggt
    23641 cgtaatcgat tggagaatga aaaattcctg ggcttttttg ctcaacgcct ttttatcatc
    23701 gcggacgcgg acgcattcgc gaatggcgaa attcggaatc gagatgcggg cgaggataag
    23761 aaagtagtag acgaaagtgt tggcccatgt gtataagccg agcgtctcat cacccaagac
    23821 gcggcagaca aacggaaagg tgataaagga aagaatcgtc aatacgattg tcctagttaa
    23881 gtttacgatg acgttagttc tggttgtctc gtgcataatc aaattgatta tatgggaaat
    23941 cgatgaaaaa ttcattgcct ttttcgattt ttataaaggc cgtaaagaaa atatgcgata
    24001 ctatgttata gtaaaaacga actatgaaaa tcctcgtcgt ctgccagttt tattatcccg
    24061 agcgcttcac cgtttccgat atatgcgccg agctcgtttc tttagggcac gatgtcagcg
    24121 tcgtcaccgg caaacccaac tactgctata acgaaatcat ccccgcatat cgcaaggtga
    24181 aatacgaaat catcgatggc gtcaaagtcc atcgcgttaa tttatatccc cgcaagcaga
    24241 gccgcctctc gattattcgc aactaccttt cctttcatcg taacgcgaaa gctttcatgc
    24301 ggcgttttaa tgagcagttc gatgtcgtct tgtcggtctc actctcgccg gtaatttcaa
    24361 ttgctccggc cgttttatat gctaaaaagc acaaggtaaa acacgtcctt cactgccttg
    24421 acttatggcc cgaatcgacg gtggtgacgg gtgccgtaaa gaaagattcg ccgatgtata
    24481 aaatgctttt tcgttggagc cgcaacctct atctggcctg cgacaaaatc atggtatctt
    24541 cgccctcatt tgccgattac ttcaaagaag tactacatat tatcgacaaa ccctttccct
    24601 atgttcccca accatcctta ggcgtgcatg aaaaccttcc gcccgcaact ttcaagaaaa
    24661 aatacaactt tgtctatgtc ggcaacatcg gcacccttca actcatcggt gaaatcgccg
    24721 aagccggcaa aatcatcgga acacggggtg atgtcgaagt tcatctcgcc ggcatgggtc
    24781 ttcaatctga aaacctgaaa aaatacatcg ctgacaataa cttaaaagat atcgtcactt
    24841 actacggacc tctgccttta gaaaaagctg tcacgcttta cgctaacgcc gacgctttaa
    24901 tcgtgccgtt aaaagaaagg ggaacagtcg gaaagacaat tcccaacaaa ctcgtccagt
    24961 atctaaaata tgctcgcccc atcatcggat cgttaagcgg cgatggccgc gatgtgctcg
    25021 tcaagacaaa aggcgcggtt ttggccgacc agaacgccga aagtatcgct aaagcgatgg
    25081 aaacaattat cgatcttccg gcagataaaa aagaagagat gggtcggcaa aataaaaagt
    25141 actacgaaga gcactttgaa attcgccgcg tcgctcaatt gattgagcgt gagctgttag
    25201 atactaaaaa ttcgtaaatc agttggccct gattttctaa accgtatttt gcgagtacct
    25261 tttcttttgc cagaagggct tttttcttca tatcgtcatt ttgaacattt aaaaagagat
    25321 tcatcgcttt gcgaagctca tcgaccgagc catccttgac ccagatggcc tctccgcaga
    25381 attcatcgcg aagagtcgta tgcagcgtcg aaagaagtgg cgcgccgctg gcaagatact
    25441 ccagcacctt gctcggcacg gaatatttat cgagattttc gtcaaacaat cgcggattga
    25501 tgttgagatc agcgtgttgc tcatatttat gaacctcttc gcggctcagc attccaagat
    25561 agtggattct agtatcttct ttagttattt cctgaatgcg ggcgattaat ggtccgtgac
    25621 cggcaatcaa tagttcgcgg tcggtcttga tttgtcgaaa ggcttcaatc atgtttaaaa
    25681 caccatagcg ctcataaagc gcgccgccga aaaagaagta cggttttgtc tcgggttttg
    25741 ttaactccgg caaggcttcg gcaaagccgc taaaaacata agagggtttg tttttcttgt
    25801 tggctaattc attaagaccg cttgataggg taataaaacc atcgtatttc tgataaagcg
    25861 acttgatggc gcgaacatag aaacgagatt catttgatag aagatgagga ttatcggtga
    25921 cgatggcaaa gacagagatg ccgtttttct tcgcggcctt aagcgttgcc tttgctaaaa
    25981 caaacttcat cgcatcgacg aggatgatgg catctttgcc aaaagtcttg atttcgtcag
    26041 ataaacattt ggtcaggctt ctgtttctat gccacaactt gtagacgcga cctgctttat
    26101 cggccaaata cttaaaagta atattgccgt ccatcgcctc ttcgcttttg agttcctgaa
    26161 aatcgaagag gcctttagca aaaggccgaa gcgtaagcgc ggtgacagcc gtttgagtgg
    26221 cgagggcttt taaaaagcgg taatggaagt tctggttgct gggattgatg tgatatttgg
    26281 cgtttttaac atacatgtta aaatcaccat ccttcatcgc tccggagaca tagagaattt
    26341 tcatcggcgt tccatccagt ctttaaaagc atcaagatgg acctttgcca tcagctcgat
    26401 cgtattttgt ccagccgtct taagcgcggc attcgccatt tcgggattat ttaatacatc
    26461 tttaatcgcc atgttaattt tgccgtcgtc attaaaatcg ataacatatc cattctcgcc
    26521 gttttttatc aggtggtgcg cggaattgac gttctgattt gaaatgacgg gaagcccttg
    26581 agacatagct tcgttgacga cgtggccgta aatatcttct ttgctcggaa agagataaac
    26641 atcggcggct ctaaaatact gaaaaacttt ttcgcggggt aagaaatcaa ggatttgcac
    26701 gttggtgagt tctagttcct ttataagccg ccggtatttg tttttttccg gtccgcctcc
    26761 gatcaagatg aggcggtggt ccgccggctg acttttccag taattaagca agcgctcata
    26821 gtttttacgc ggaatgaatt gaccgaccga gacaaaaatc ttcgcgccgg tgaggttgag
    26881 aatcgttctg agggccgctt tttcttggct atcaagcggc tttggaagga tctccctttc
    26941 atatagagtt gaatatggat aattgatgat gtggttggcg ttcgcgccgt aatgcacaag
    27001 atacttgttg gagttttcat ctggtgaaaa gtagtggtcc gcgccgctga tgtagtgtgt
    27061 tttaatgcgc ctcaacacgc ttgattcgcg cttgcggata atgccgccgt taatataaaa
    27121 aacatatgat ttgcggttct ttttcagata attaagagcc gccatctcac tgaaggtgtg
    27181 ccacccattc atgatgatta tgtcgtattg attgttcttg aggtgtctga ccactcctcg
    27241 agagagatgg ttatcatgac cgagatttag ccctttgata tagacggtct taaatttgat
    27301 ttcctcgccc tgataaaagc gcggatcgcg gtcgctgtct ttcagccttt caaataaagc
    27361 ggtgacctcg atgtctttac tcagttcgtt aaaaagcctt actttgtaag gagcctgatg
    27421 attaaaaaca aagagtactt tcacactaaa atcctaatga taattataca ttgtatcaat
    27481 atgcttatat aataaaatat caggaaacaa acactccatg agctgggact tattt
//
LOCUS       DAOSSL010000010        27515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_1925_length_27515_cov_18.345666, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000010 DAOSSL010000000
VERSION     DAOSSL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 27515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27515
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_27515_cov_18.345666"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLH17_01930"
     CDS             <1..297
                     /locus_tag="PLH17_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20575.1"
                     /translation="QHATYLLLLATNGQLKIKELNALSDNDAALTTRALKLLKEKNLV
                     DRTCDSVRKCRIFLTDKGKDVVKYIGEIFSRLRDELAKSVAAEGQETLLSAILS"
     gene            318..1604
                     /locus_tag="PLH17_01935"
     CDS             318..1604
                     /locus_tag="PLH17_01935"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR20576.1"
                     /translation="MELIIENALRIYLLVCLFLLILYIPRIAYYFSAFRDQPRLINPA
                     KNRLAVLVPAKNESKVIQALLDTLNLQTYDRAFFDVHVIVEDARDPSVGMAQKAIDAT
                     IHVVPDQTRKGEALDGALKKILKDTPSKYDAFVIIDADNLAHPDFLLEMNNALASGRQ
                     IVVGKKRIKNWESANRADRSLFSNCTALVYAMVDDLGNRHRTKHNIPVSMCGTGMMVR
                     ADVIKEQNGWPYRTLTEDYEVMMDCFLRGYTSMYYMHAIVYTEEAVEHRTSFKRRMRW
                     LTGYTQCNKKYRRAVVKKTFGEGKIVWKNFDFLYSLLPVFIFAGISLVLGITCLFAAG
                     AYAIFFQPLFWPILRVAIISFGTVYGVLMLFTILTLIVDRKALKMSFFELVAVIFLNP
                     IILAEFIWIFIRIFTATNNLEWDNVDRIAYTPKKDK"
     assembly_gap    1699..1798
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1799..2113
                     /locus_tag="PLH17_01940"
     CDS             <1799..2113
                     /locus_tag="PLH17_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20577.1"
                     /translation="PRPFRNGAFHFAATANVPVIPIFLTFSDPKGLRAKIVKSKVVTV
                     HIMPPIYPKEHYSVKENVDYMRESNYELCVHKYEEVYLRPMVYDVVWPVDEAGEGQTT
                     AS"
     gene            2135..2851
                     /locus_tag="PLH17_01945"
     CDS             2135..2851
                     /locus_tag="PLH17_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOR20578.1"
                     /translation="MKISMSEKTATITKIVAFVIVFGTLIGLFIAYFDFFYSLLEVVN
                     RQALRDRMLGMGIWGYGLAFLIQFTQVVISILPGEVVELFLGMAYGPFIGLIIAVLGV
                     LAGTSVAYYLAKWVGIPFVKLFLKKNYFEKYAFLQDEKKMALGIFIVYLVVGLPVDTA
                     TYFAPTLKIKFGKFLLLSTLARIPKIISSTIVGHYLVEDNLLVSLIVFFGTALLSVIA
                     VIIFRFVSRRKQQKASIDKA"
     gene            2937..3422
                     /locus_tag="PLH17_01950"
     CDS             2937..3422
                     /locus_tag="PLH17_01950"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR20579.1"
                     /translation="MSKLIILSGIPGSGKTTYALGIKNILPRVAIISSDEVRKDVTGD
                     ACDGSQETRVWQEFEKRVQQASKGHDIVIADSTALTNLLRMKWYRAFRDAFEKIELVY
                     LNLPFAVCWERNIARERKVPLNPMAGMFKLLEEPNEEVNNSFDSIIVIKEEDKDTWKR
                     S"
     gene            3407..3868
                     /locus_tag="PLH17_01955"
     CDS             3407..3868
                     /locus_tag="PLH17_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOR20580.1"
                     /translation="MEKKLIVLSGIPGSGKSTYAADYVNKNPETYIVASDAIRLELFG
                     RVDDFSKEELVWDIFENRIREKAKTHSAVIADSSATTNVRRLRWASIFRDYFDIIELV
                     YFDIPFVVCLERNEKRHLSIPYDDMVVMRDSFEKPSDEVVGAYDKITEIKR"
     gene            complement(3886..5148)
                     /locus_tag="PLH17_01960"
     CDS             complement(3886..5148)
                     /locus_tag="PLH17_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOR20581.1"
                     /translation="MIGASLFKPDLKIKKLSINTYWIIPLLGALVLLIAGLISLPEIF
                     AGLSADTAINPLKILALFLSMTLMSIFLDEVGLFRFLASFALKKAKTSQMTMFIILYA
                     LVSFLTMFTSNDIIILTFTPFICYFAKRAKINPLPYLFASFVAANTWSMMFIIGNPTN
                     IYLASSHGISFLDYVQVMYLPTIFAGLASFGTLFLLFRKQLSAPLGEEVETVQIKDKF
                     LLIAGLFALGGCTILLTVSNYIGLEMYLITLIFAAALVVIVLLYKAFRKSKSTELKTT
                     LKRLPWDLIPFISSMFVIVLALNKHGATEYLAAFFSDTKPIIKYGVSSFLMSNFINNI
                     PMSVLFSSVTAYASDAIVLPAVYASIIGSNLGAFLTPIGALAGMMWMGLLKSHQVKFT
                     FLKFVELGVFPAVVALGAALLGLFIVLL"
     gene            5352..6326
                     /locus_tag="PLH17_01965"
     CDS             5352..6326
                     /locus_tag="PLH17_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR20582.1"
                     /translation="MALIVSDAWKKKYQIAEELKDYARFMTLGSIKITPAKIALSNTG
                     AALTTRAPVIDLRVKTIKKRIPTYDGKSIGLTIYEPKNIKEEKAPAILYLHGGGFMIR
                     EASHHHKVCRDYAVGAKAKLVFVHYRLAPRHSFPYPVEDCYSALLWLARNASRLGIDK
                     KRIAVAGDSAGGGLAAALTHMSRDRNGPKLCFQMLVYPALDHRQKTTSIKEYYDAPGW
                     NSLLNKQMWKIYLKNGHFGMLSYASPAVSTCFEKIPSAYIEPAQYDCLRDEGFEYARK
                     LEDHGVSVELNYVEGAFHAFDIMQSKKLVRKVMAQRISALSRALHPSK"
     gene            6391..>7226
                     /locus_tag="PLH17_01970"
     CDS             6391..>7226
                     /locus_tag="PLH17_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol acetyltransferase"
                     /protein_id="HOR20583.1"
                     /translation="MKKKINGINQDWYRLDNAAKIYPAMVSRRNSCVFRFAINLKEEV
                     DPYVLQRALLDMRNRFPAMFVRVRHGVFWYYLEENKKMPRVRPEDSRINKAIDYYSNN
                     YFRFTVFYYENRISLEVFHSLTDGTGAVTFLKALAYRYLELKGHKLESEGLVLTVDQD
                     PSIEELEDSFFKYATMNPHKRFVVPTPFSIRDARFPSGQGRGVINARLDSAALYALAK
                     KHDATVTEYLSALLTQAVYQVYGDKMIKNDRVNICIPINLRRLFPSDSLRNFTLFFHT
                     TVT"
     assembly_gap    7227..7326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7502..8230
                     /locus_tag="PLH17_01975"
     CDS             7502..8230
                     /locus_tag="PLH17_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOR20584.1"
                     /translation="MDYCPECKVQVVSKGEKCPLCQGPLDRGEKPASKKPFPSYIKFV
                     NLRKRWARGISLAAPVAIGICVLINLLTWNGFLWSLIVGVAILYAWLVGLYTVRRTDH
                     LGQKLLLHAIGISALLIVINFVTASPSMTSSEFLENFSEIARWSLDYAMPATLMAFIL
                     AINIATLSQRHRLRDYIIAQFSLSFIGFIPFVLIMIDIFAADILGSLFMSIAASSFSL
                     LTLLVFVIFAPKLVAKEFGRRFHI"
     gene            8243..8578
                     /locus_tag="PLH17_01980"
     CDS             8243..8578
                     /locus_tag="PLH17_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20585.1"
                     /translation="MKYNHPETALVTIIGPDQTSWIGDIKVEVLFDGEIVGVIKGQKS
                     VQFEINEDTRFLIRTANEDLPKSEVTVAGLFRCPTTIKLYLLGSSPANAKLVAKTTYG
                     ENYSKRKYL"
     assembly_gap    8682..8781
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8782..10241
                     /locus_tag="PLH17_01985"
     CDS             <8782..10241
                     /locus_tag="PLH17_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOR20586.1"
                     /translation="AEPLPPPVVSDINGEEISHEANDGDIIKLMHMNDTHGSIEYLPD
                     NNEPGMAYIAGYVNEKRAQENTDVVLLSSGDMFQGSLDSNINQGKLMIDIMKEMRFDS
                     MSIGNHEFDWGVDVLAENAQYALDSDTGDWAFPFLSGNILGPEGQYEFGHLSTTFNRG
                     PARISIIGSIDSGVYDSIDGPIVAGYEFVNAASMVISEAQRLRASGSDIIIYSTHDVD
                     KRVAQTIANNVDAIFTGHNHHNSVLTMTSSTSKTIPIIESSSNGRALGEVDFIYDAET
                     DSYELGTYKNTNVLDGVISLIEDDGVQAIYDRYLDAPAQDGPVSSDSLRSLKSDKVGE
                     ITSDSKYVAYDNVSIPKSDVATMFLYAQLEAYIDSYDIVGSCYNESRSAWIVGNITYA
                     DIYKAFPFDNATVIVAATGAQLKTWSTTTVYVDGISKTTLDNNTVYQIVTSTYIINNR
                     EEGKFNSIVNIDEDLFQRHVFYQQFLEATTPNPWG"
     gene            10311..10577
                     /locus_tag="PLH17_01990"
     CDS             10311..10577
                     /locus_tag="PLH17_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20587.1"
                     /translation="MVKRIFGIIFALAGLAAMVVAVIFGIQAVQELSAYIAGGVDPLA
                     PIAFVVFIVMVAVWVALFIIGVVVFIPSLIVAIKGPKKKKPISG"
     gene            10624..11262
                     /locus_tag="PLH17_01995"
     CDS             10624..11262
                     /locus_tag="PLH17_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20588.1"
                     /translation="MNENKLTEKKSEENSQTKKKGRWISYLPAFFIALITIALLLLII
                     IGTIFSGGENQDAQVRALHSINFTGETAEFNDDYIYEPAVSEEPVFVAEQGFRWDSLA
                     SKIVVRVPEYREAVSINIGFGLFNFKAYSEVETSSDNYAIQAFDGDGALLETKMLTNV
                     QNDSEQQVVFQLEDVKRLTITFQEPITNIGEEVTSGYLYLKDFSTCEIINYE"
     gene            complement(11253..12404)
                     /locus_tag="PLH17_02000"
     CDS             complement(11253..12404)
                     /locus_tag="PLH17_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HOR20589.1"
                     /translation="MNGKDKLKKAVFAKIYVLIAVILLFAALIVIRLFTASAEFWTRT
                     FYRFIQSFFGPLASLIPFSLAEVFFILFGVTIITLIVLIIIDFSKKRGLIGLNKTLAL
                     ILVATSTFAYYFATAGVAYGRYPIDIPQHQEDVESSEYVEIIEHFLADFNACADELEF
                     TAEGSVIRSYSIRELSEILKIEYARLESDYFTSFTPNVKPMFLSFLFREFNITGISFA
                     PTTEPNINYLTPDSQLVSTMAHEIAHSKGAMREQDANLVAAYILLNSDDPYLRYSGYF
                     TTFYSIISLSNYVGDEDKYGELSNSLRDEIKGDYQYSRSYWEKYDFLDDMATWFNNLY
                     LMIVGNNGVSSYVDTPDIDETTDPDTGDPVYYIKEYSPYQKLYFYFYYS"
     gene            complement(12437..13483)
                     /locus_tag="PLH17_02005"
     CDS             complement(12437..13483)
                     /locus_tag="PLH17_02005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOR20590.1"
                     /translation="MKKIIYYITGATGHLGRTIVNLLLSQKKTIVAFILPHDQKRDFE
                     NQNQGELYFVEGDIRKDEDVEKFLSFKSEDAAQIVIHCAGIITVENSFNQLVYDVNVN
                     GTKRITDSAKKRNVEKFIYVSSVHALKELSKKDLITEQDHFDPNEVNGFYAKTKAEAS
                     QYVLDAVKNGLRGIIVHPSGIIGPNDEMNGHFTAVLKNYLDGSLTSIVKGGYNIVDVR
                     DVADGIIKATDRGVEGRCYLLSGQYHTIYEIIEIASRISQRRKIRSVLPIWFVKMFAP
                     LAALWSKMKKQPPIFTAYSVDTLVSNSNFSNALAVRELGFTVRPIFNTIEDTVRDLMS
                     RQDKNAKSSKKTRK"
     gene            13606..14094
                     /gene="coaD"
                     /locus_tag="PLH17_02010"
     CDS             13606..14094
                     /gene="coaD"
                     /locus_tag="PLH17_02010"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOR20591.1"
                     /translation="MVKKAIYPGSFDPVSNGHIDIIERISRLFEAVYVVVAVNPNKRY
                     IFTADERVEMLKKATAHLHNVVVEKSDLILVDYVRLKGEGVIIRGVRNVNDYQNEITM
                     YQFNHTSDPNIETLIMFPSSHNLFISASYIKELALFNADISPYVPKGLADFISRTIRA
                     RK"
     gene            14178..15092
                     /locus_tag="PLH17_02015"
     CDS             14178..15092
                     /locus_tag="PLH17_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR20592.1"
                     /translation="MFIFRPSLKIFKRTLDIYEEKRIKKIKPFAEMRGWKDVPYIDDG
                     SVEHLLDIYHPAENGNNHLIFDIHGGSYAFGFKGLNYIFNSYFVAKGFSVVAINYTLV
                     KKGTSIYNQILDIFAALHFLEKNKEKYNLSFDNFILLGDSAGGHLALLADLVFKSPEA
                     QEYYKIKSLPNIKIKCVAMNCAVYDFGEVVDFGKRVIHKSAMPYLFSSKYLDGDFLKM
                     NNPAYYIRKVKPDPIFISSCRNDFLKKHSFMLRDELLRQGLIHHYEFEPTLDVKVAHV
                     YNLVLPDSKQGKRTNDAMADFFLRDFTK"
     gene            15168..17786
                     /gene="aguA"
                     /locus_tag="PLH17_02020"
     CDS             15168..17786
                     /gene="aguA"
                     /locus_tag="PLH17_02020"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HOR20593.1"
                     /translation="MKLNQNQTPFLDALIKYVSENISPFDVPGHHMGNVENRLKDFLG
                     AKTFRSDVNAPIGLDSLQHPTGVIAEAQELMADFAGADHSFFLVNGTTSGIIAMIMAV
                     CKAKDKIILPRNVHKSIISALVLSGSIPVYVMPKIDRQLEIANQPSADDYKRAIIRYP
                     SAKAILVINPTYFGAVNDLKELTAFAHAHNVTVLVDEAHGAHYYFSKNGPLSAMACGA
                     DLSSVSFHKTGGSLTQSSVLLLQGKRVSPSEVQKSLSIINTTSPSNLLIASIDAARHF
                     AATKGQEAMDQVLELAQHAREQISKIKGFIPRGREHFLQKGCYDYDETKLVIELDHLD
                     LSGFDLYYLLKEKYRIQVELAETYVILGILAIGTKKEHLKHLFAALKEISRDHYNRKI
                     TYPRHSFSVGFPFLLVRPRSAFYAPSKRISIMDAANEISKESIMIYPPGIPLIIPGEI
                     FTNDLIERIKSYKQTGITMISDYSDGTVNVVDKEHWKRFSSYQKAYQDYSSKRITTPH
                     NDGYSMPFEGDAHQATFMLLPFRKDTWREGAKPAREAFKDVIRAIAQFEPVIVGIHPS
                     IYDVASGEFSNIDNVTVIKIKYNDAWARDNAPIFVKKGTKIRSVDFRFNAWSGEDGGL
                     YSNYYDDDRLAGVLSKKLNINSYYIEDFVLEGGSIHVDGQGTCLVTEACLLSKGRNPH
                     LNRQEIEETLKTNLGVSKVIWIKNGIYQDETSEHVDNMACFVRPGVIALAWTTDRKDP
                     QYKYSQAAYKVLKSETDADGKPFEIIKVRLPHPMYMTREEAKGIRGSRSNAKKREPNM
                     RLAASYINYYQGKDFVILPAFGVKEDKLAYEQFSSLYPDKKIMQVNSREILLGGGNIH
                     CITMQIPETKKGEQQL"
     gene            17783..18643
                     /gene="aguB"
                     /locus_tag="PLH17_02025"
     CDS             17783..18643
                     /gene="aguB"
                     /locus_tag="PLH17_02025"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HOR20594.1"
                     /translation="MKVAITQFKMSWNIDENLAKAEQMVRKAAQKGAQVILLPELFKT
                     PYFCQRENYEYFDLAEELDSSTTISRFQKLAKELNVVLPLSFFEKAGNVHFNSLVMID
                     ADGKNLGLYRKSHIPTGECYEEKFYFSPGDTGFKVFKTKYGRVGVGICWDQWFPEVAR
                     SLALMGAQLIVYPTAIGTEPVLPKDSKNHWQNTMRGHAAANIVPVLASNRIGTESDKE
                     SSMTFFGSSFIAGEDGNTVIELSRNEEDVAVAEFDLAEIDKKRIAWGVFRDRRPDLYH
                     SITKFDDSRK"
     gene            18648..19091
                     /locus_tag="PLH17_02030"
     CDS             18648..19091
                     /locus_tag="PLH17_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOR20595.1"
                     /translation="MGLHAFRAGANALCYVKNDHKYGMICAWAMMIDYDVIALLVGEQ
                     SATGKNLEVGMLVGVSALSKEQKAVALTLGNGHSDEIDKFANLSYEIDESAILIRGAR
                     NNLVVRVEKLIKLNPENQDILVIAKFAKSKEDKSKEFIDAEEVFR"
     gene            complement(19094..19462)
                     /locus_tag="PLH17_02035"
     CDS             complement(19094..19462)
                     /locus_tag="PLH17_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOR20596.1"
                     /translation="MKKTSKAIEDYLEAFYILYNNKEPLESARVSRLLNVSRPAVNKA
                     THELMRLGFINKTPYSEIEFTPSGKRIAKKVYHRHTTLKKYLLSLGVSEKNADRDCCL
                     IEHVLSEETFKKIENLVKEK"
     gene            19610..19900
                     /locus_tag="PLH17_02040"
     CDS             19610..19900
                     /locus_tag="PLH17_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOR20597.1"
                     /translation="MQERNRHQHGKRQRLQTGQEGHLSDLDRGQKAIVLDFHNDNAAL
                     RRRLLDMGITKGVEIEIKKIAPLGDPVDIRLRGYELCIRRKDMRSIDIKVVK"
     gene            19903..21933
                     /gene="feoB"
                     /locus_tag="PLH17_02045"
     CDS             19903..21933
                     /gene="feoB"
                     /locus_tag="PLH17_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOR20598.1"
                     /translation="MKIALVGNQNSGKTTLFNTLTGTNQKVGNWPGVTIERKEGKISG
                     TEIEIVDLPGVYSLSPYSTEEEISRQFAMEEKPDLIINIIDATSLERSLYLTTQLLEL
                     DVDVIIALNMADVLEKSGITVDIECLSRRLNTTIISISAKKKLGVDNLISVIKNGEYK
                     KNHHDYIYDEEIENVISGITSQIKVGHKRFSAVKIIERDRDYRPLLTDEITAKINALE
                     EMYKMDGEQLIADQRYRHIVRIKKECSTHTEKGETITDKLDRVFLHKYFGLPIFALIM
                     GIVYFLSVGLVDGLTVDFVAALFDGAETIDVLGTEVPFAIAGLGPTLSAWLTNVGASP
                     WSVSLVVDGIIAGFGAVSSFLPQLIILFLCLALLETTGYMSRISFFLDRIFKRFGLSG
                     KSLIPFIVGSGCSVPGIMASRTVETEEERKMTVMLTPFVPCSAKLPIIALFAGYFFST
                     YAWLVTLSLYFMAIIVILLSALILKKFVYKGATSSYLSELPNYHAPSALYVFRDVFDK
                     TWSFIKRAGTVIVLCSVAVWFLASFTWTFKYIDGVNFHIENSILAGIGNAFAWIFYPM
                     LGGTWSWGATVSAIQGLIAKEQVVSSLEVIAGLEGGGASVFESGIFSFFTGWSAYAYM
                     IFNLFSAPCFAAIGAMRKELGSTKGMLKAVLFQTGIAWLIATFVGIIGWLII"
     gene            21930..22106
                     /locus_tag="PLH17_02050"
     CDS             21930..22106
                     /locus_tag="PLH17_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20599.1"
                     /translation="MTWVDGVILGIVVVLLGLIIYFSLIKNREKGSCRNCPEANGVKA
                     NRLLKDYKRKFKKK"
     gene            22168..23031
                     /locus_tag="PLH17_02055"
     CDS             22168..23031
                     /locus_tag="PLH17_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HOR20600.1"
                     /translation="MNIEKVALVLQGGAVRGAFTAGVLDVLMENDLYFQYVVGVSAGA
                     LISLSYLSKQIGRSRSVVVDFMADKKFVNPRFLLTKRSLFDFDYLFGDIATKILPFDF
                     DTYEKSPIKFVVGTTSCHSGQPLYFIKDETPDFFKAIAASSSLPFFSAPVFVGEEPCL
                     DGGIATPIPFQKALDDGYKKMIVITTRDKNYRKDLDKPQHKNGLKALYGKYPKFIEAA
                     LEYPRRYNQDAARLEELENLGMAFVVRPRLPITLKRTEKDRAELAKVYERGIAVATEL
                     LPKIKEYIKNE"
     gene            23024..>23702
                     /locus_tag="PLH17_02060"
     CDS             23024..>23702
                     /locus_tag="PLH17_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOR20601.1"
                     /translation="MNKNDKSRPMTRSREVDPKNIKEFAVKEPCELLTFLLEKLPSLS
                     RNNVKRLLANNQVAVDGAPIRQFNFLLAKEDVVIVSKNRITSKKRENLPIIYEDEEFI
                     VIDKPSGLLSVASDKVKGRTAYRMLMDYVQQKDRHNRIYVVHRLDEDTSGVMMVAKNV
                     EIRDALQKNWASIVTSRGYYAIVEGAMEKKEDTLHHYLAENNLNLMYVTTNKKIGKEC
                     TTRYKTIS"
     assembly_gap    23703..23802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23803..25361
                     /locus_tag="PLH17_02065"
     CDS             <23803..25361
                     /locus_tag="PLH17_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOR20602.1"
                     /translation="YIFMSFSWLNKFKAALISTLPMVVIVLIIHFTGLAKIDDSKDVI
                     IFVISAVLVALGMGLFTIGTDLAMSPIGEYIGASITSQRKIGLMVFLFFLLGAFVVIA
                     EPDLRVLVGQVPLGGQWLPFISNEWLIIILIGVGFGAFMVVGILRILFQKSLVIWLLL
                     FYGLLFSLALGVNYVNPSFIPLSFDSGGASAGTITVPFILALGIGIATTRSGKKTDED
                     SFGLIAITSLGPVLIVMIMSFFIEDIPFIYEPEAAQSFGAILKTSALNVAIAILPITA
                     FFFIYQAIAIRLPLKVLIRIISGLVYTYLGLVIFLTAVNLGFLPISRIIGSKIAAQSS
                     SLVILFGAIIGMAAVLLEPAIHVLVSQVENITEGTIRKGAVLAAIAVGAGLAMSFAMM
                     RLVFGITILWFVIGGYLVALGMAFFVPKIYSAMAFDAGRITSGPMNSSFVLPFAIGAA
                     ITFYQSDGQDIMTYAFGVNALVTMVPIITIQALGLYVTAQEKRRIRLAGMRFVEENDD
                     QIIYLLEDEQ"
     gene            25358..25780
                     /locus_tag="PLH17_02070"
     CDS             25358..25780
                     /locus_tag="PLH17_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20603.1"
                     /translation="MSKKKVASNPSLPVESKPETFKSVDRVQPLSRLKFFITIVNHNH
                     YKVVLKILKNHESGLGFITHGRGTGTKEIYDLLGLSDVHKDIIFAVVKADQIETLKKE
                     IGAFFVKESKGIAFAIDISSVIGSSIYKYLSNTRPTGE"
     gene            25783..26088
                     /locus_tag="PLH17_02075"
     CDS             25783..26088
                     /locus_tag="PLH17_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOR20604.1"
                     /translation="MEKHELIICIVNNGFTDLVMDAARKEGARGGTIFHGRGTGNPDM
                     EKFFGFSISPEKEIVIILVEEAIKDAVLKAINKAAGLETNGKGIAFSLPVSDTVGVN"
     gene            complement(26091..26606)
                     /locus_tag="PLH17_02080"
     CDS             complement(26091..26606)
                     /locus_tag="PLH17_02080"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR20605.1"
                     /translation="MDILDTIKKRQSVRDFSEEKISEEHLKIILDAAYHAPIGRAAYN
                     RYELIVIKHKEKIDALAQKIAEAGILTKHPFFHAPIVIFVAAKPNRERLDGCDTGCLI
                     ENMMLAATSLGLGSCFLYTVSEVINGRPFLLQALGLSDEYRAMSAVVIGHPKEKDLPP
                     KVRPGIATRVL"
     gene            26664..>27515
                     /locus_tag="PLH17_02085"
     CDS             26664..>27515
                     /locus_tag="PLH17_02085"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOR20606.1"
                     /translation="MARGWFRFKQFIFKKMPYLKDRGSLFYYGLFLAAIGLGFYGYSL
                     LTQYFSVPLGGDYTQQYVAFHYNFYDDWWTFFKTGDFVMWDHNTFLGVDNITSNTYYG
                     LFSPFMLPILLFPRYFIPHLLAVTMIARMVTGGLLFRLYLKHFNVKEGTARLFAIAYA
                     FCGWIAYYLWFNNFFEVIAFFPLVLLGIEKILNREKPYTLIIGLFLIGLTHYFLLITI
                     CFYGVAYALFRFFQVRKQHTRKENWIILGWGVGSFAVGLMLAAWVLIPNYLNAMQAPR
                     AEDATYLL"
BASE COUNT         7276 a         6362 c         6087 g         7390 t
ORIGIN      
        1 cagcatgcaa cctatcttct tttattggcc accaacggtc aattgaaaat taaagagcta
       61 aatgctcttt ctgataatga tgcggcgctt acgaccagag ccctcaagct tctcaaagag
      121 aagaatctgg tcgatcgcac ttgtgacagc gttcgcaaat gccgaatctt tcttacggat
      181 aaaggaaaag acgtcgttaa atatatcggc gagatttttt cacgactccg cgatgagctc
      241 gccaagtcgg tcgccgctga aggccaggaa acactcctca gcgccatttt aagttaggca
      301 agaaaaggta agaaacaatg gaattgatta ttgaaaatgc cttgcggata tatcttttag
      361 tttgcctctt tcttttgata ctttacattc cgcgtatcgc ctactatttt agtgccttcc
      421 gcgaccagcc acggcttata aatccggcga agaatcgcct cgctgttttg gtgccggcaa
      481 aaaacgaaag taaagttata caagcgctgc ttgacactct taacctacaa acttacgacc
      541 gcgcattttt tgatgttcac gtcatcgttg aagatgcgcg cgacccctcg gtggggatgg
      601 ctcaaaaagc catcgatgcg acgattcacg tcgtccccga tcagacgcgg aaaggcgaag
      661 cgctcgatgg agccttaaaa aagatactaa aagatactcc aagtaaatat gatgcctttg
      721 tcatcatcga cgccgataac ctcgcccatc ccgattttct tttagaaatg aacaatgcac
      781 tggccagtgg acggcaaatc gtcgtcggca aaaagcgcat caaaaactgg gaatcagcga
      841 accgcgctga ccgctccttg ttcagcaact gcaccgccct cgtctatgcg atggtcgacg
      901 atttaggcaa ccgccatcgc accaagcaca atatccccgt tagcatgtgc ggcacgggta
      961 tgatggttcg ggccgatgtc atcaaagaac aaaacggctg gccgtaccgg accctgaccg
     1021 aagactatga agtgatgatg gattgctttt tgcgcggcta tacatccatg tattatatgc
     1081 atgcgattgt ttacaccgaa gaagccgtcg agcatcgcac ttcttttaaa cggcgcatgc
     1141 ggtggctgac cggctacacg cagtgcaaca aaaaataccg gcgcgcggtc gtcaagaaaa
     1201 cttttggcga gggaaaaatc gtctggaaga atttcgattt tctctactcg cttctccccg
     1261 tttttatctt tgccggcatc agtttggtac ttggcattac atgtcttttc gcagccgggg
     1321 cttatgctat cttttttcag cctctttttt ggccgattct tcgcgtggcc atcatctcct
     1381 ttggaaccgt ttatggcgtt ctgatgctct ttaccatcct cactctcatc gtcgaccgca
     1441 aggcgctgaa gatgagtttt tttgaactcg tcgccgtcat ctttctcaac cccatcatcc
     1501 tcgctgaatt catctggatt tttattcgca tatttacggc gaccaacaat cttgaatggg
     1561 ataacgtcga tcgcatcgct tacacgccaa aaaaggataa ataatcatgg ctcttcctaa
     1621 ggaagaattg ctcgaacgca tccgcgatgc cgaaaggcgc ggcgactttc attttgtcgc
     1681 tgagcccatc gacgagaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     1801 gcgccctttt cgcaatgggg cctttcattt cgccgcgacg gcaaacgtcc ccgtcattcc
     1861 gatttttttg acattttccg accccaaagg actgcgcgct aaaatcgtca aaagcaaagt
     1921 cgtgaccgtc cacatcatgc cgccgattta tcccaaagag cattatagcg tcaaggaaaa
     1981 tgtcgattac atgcgcgaat ccaactacga gctttgcgtt cataagtatg aagaagttta
     2041 cctgaggccg atggtttatg atgtcgtttg gcccgtcgat gaagcaggcg aaggtcaaac
     2101 gacagcctca taaagaaaat gctataatgg cggcgtgaaa atctcgatgt cggaaaaaac
     2161 ggcgaccatc actaaaattg ttgctttcgt catcgttttt ggcacgctta tcggtctttt
     2221 catcgcctat tttgatttct tttattcgct tcttgaagtc gttaatcgcc aagccctgcg
     2281 cgaccggatg ctcggaatgg gcatctgggg ttacggattg gcttttctca ttcagtttac
     2341 ccaggtcgtc atctcgattt tgccgggcga agtcgtcgag ctctttttgg gaatggccta
     2401 tggtccgttc atcggtttaa taatcgccgt actcggcgtc ctcgccggaa cgagcgtcgc
     2461 ctactatctt gccaaatggg tcggcatccc tttcgtcaaa ctttttctga agaaaaatta
     2521 ctttgaaaaa tatgctttcc ttcaagatga gaaaaagatg gccttaggca ttttcatcgt
     2581 ctatttggtg gtcggtcttc cagttgatac ggccacgtat ttcgcaccga cgctcaaaat
     2641 aaagttcgga aaatttctcc tgctttcgac tttggcccgc attcccaaga tcatctcctc
     2701 gacgatcgtc ggacattact tagtagagga taatttactc gtctcattaa tcgttttctt
     2761 cggcaccgcg cttttaagcg taatcgccgt tatcatcttc cgttttgtca gcaggcgtaa
     2821 acaacaaaaa gcatcgattg ataaagcata atttaagtcc gctcttgaga aaggtttccg
     2881 cttttgctat ggtatttgtt gttgatatcc atcattaatg atggcgaggt ataatcatga
     2941 gcaaattaat tatcctgtcc ggcattcccg gctcaggcaa gacaacttat gctctgggca
     3001 tcaaaaatat tttgccgcgc gtcgcaatta tctccagcga tgaagttcgc aaagacgtga
     3061 ccggagacgc ctgtgatggt tcccaggaga cacgcgtctg gcaggaattt gaaaaacgcg
     3121 ttcagcaagc ttcaaaagga cacgacatcg tcatcgccga ctcgacagct ctgaccaatc
     3181 ttctgcggat gaaatggtat cgcgcctttc gcgatgcctt tgaaaaaata gaactcgtct
     3241 acctcaattt gcccttcgct gtatgctggg agagaaatat tgcccgcgaa cgcaaggtgc
     3301 cgttaaatcc gatggccggg atgtttaaac tccttgaaga gcccaacgaa gaagtgaata
     3361 atagttttga ttcaatcatc gtcatcaaag aagaagacaa ggatacatgg aaaagaagct
     3421 gatcgtcctc tcgggaattc cgggttcagg caagagcaca tacgctgccg actacgtcaa
     3481 taaaaacccc gaaacttata tcgtcgcaag cgatgccatt cgcctggaac tttttggccg
     3541 tgtcgatgac ttcagcaaag aggaattagt ctgggacatt tttgaaaacc gcattcgaga
     3601 aaaagctaaa acgcatagtg ctgttatcgc cgattcgtca gcgaccacga atgttcgccg
     3661 ccttagatgg gcgagtattt ttcgcgacta cttcgatatt atcgaattag tttatttcga
     3721 tattcccttt gtggtttgtt tggagcgaaa tgaaaaacgc catctgagta ttccctacga
     3781 cgacatggtg gtaatgcgcg attcgtttga aaaaccatct gatgaagtcg ttggcgctta
     3841 cgataaaata acagaaatca aaagatgagg aatcccccgt cttttttata gcaaaacaat
     3901 aaacaagccg agtaaggccg cacccaaagc tacgacagca ggaaaaaccc ccagctcgac
     3961 aaactttaga aaagtaaatt ttacctggtg gctttttaat aagcccatcc acatcatgcc
     4021 cgccaaagca ccgatcggag ttaaaaaggc ccctaagtta cttccgataa ttgaagcgta
     4081 gacggcgggc aggacaatcg cgtctgacgc gtatgccgtc accgacgaga aaaggacgct
     4141 catcggaatg ttgttaatga agttggacat taaaaatgaa gagacgccat atttgataat
     4201 gggtttcgtg tcagaaaaga aagcggccag atactctgtg gcgccgtgct tatttaaagc
     4261 aaggacaatg acaaacatcg agctgataaa gggaatcagg tcccaaggca gccgtttgag
     4321 cgtcgtcttt agttcggtcg atttagattt tcggaaggcc ttatagagca agacaattac
     4381 gacgagggcg gcggcgaaga tgagagtaat caagtacatt tcaagtccga tataattaga
     4441 cacagtcaag agaatcgtac agccgccgag agcgaacaaa ccagcgataa gcagaaattt
     4501 atccttgatt tgcacggttt ccacttcttc tccaagcgga gcggagagct gtttccgaaa
     4561 gagcaaaaag agggtgccga aggaagctaa accggcaaaa atggttggca gatacattac
     4621 ctgcacataa tccaaaaatg agattccatg gctcgaggct agatagatat tggtcggatt
     4681 tccgatgatg aacatcatgc tccaggtatt ggcggccacg aagctcgcaa agagataagg
     4741 cagcggattg attttagcgc gtttggcgaa atagcaaata aagggcgtaa aggtcaaaat
     4801 gatgatgtcg ttgctcgtga acatcgtcaa aaacgagacg agggcataga ggatgataaa
     4861 cattgtcatc tggcttgttt tagctttttt aagagcaaac gaagccagaa aacgaaaaag
     4921 tcctacctca tcaagaaaga tggacatcag agtcatcgat aagaagagag ccagtatctt
     4981 cagcggatta atcgccgtat cggcgcttaa ccccgcgaaa atttccggaa gcgaaatgag
     5041 accggctatc aacaaaacaa gcgcgcccag caaaggtatt atccagtaag tattaatact
     5101 taacttctta atttttaaat cgggcttaaa tagggaagcg ccgatcatgg taaggattgt
     5161 aagaagcgac aaggcaattg tccaagccac gataagcacc tacttttcaa gccgatttgt
     5221 tgttcggtaa gataaatctt aatccttttt gtatcttttt caatatcttt gataatcgac
     5281 cccatttttg gcggcgacat gctaaaatag taggaccatt tagatatcat attaaatagt
     5341 aagaggaatt tatggcgctt atcgtatccg atgcctggaa gaaaaaatat cagattgccg
     5401 aagagctgaa ggactacgct cgcttcatga ctttgggctc gattaaaatc acgccggcaa
     5461 aaatagcctt gagcaacacg ggagcggccc tcaccacccg cgctccggtc atcgatctgc
     5521 gcgttaaaac gataaaaaag cgcattccga catacgacgg caagagcatc ggcctcacga
     5581 tttacgaacc gaagaatatt aaagaagaaa aagcgccggc catcttatat ttacatggtg
     5641 gaggtttcat gattcgcgaa gcgagccacc atcataaagt ttgtcgcgac tatgccgtgg
     5701 gagcaaaggc taagctcgtc tttgttcatt accgactcgc ccctcgccac tcgtttcctt
     5761 atccggtgga agattgctat tcagctcttc tttggctcgc gcggaatgcc tcgcgactag
     5821 gcatcgacaa aaagcgaatc gccgtagccg gtgacagcgc cggaggcgga ctcgcagccg
     5881 ccctgaccca tatgagccgt gatcgcaacg gaccaaaact ttgttttcag atgttggtgt
     5941 atccggcgct tgaccaccgg caaaagacaa cttccatcaa ggagtattac gatgctccgg
     6001 gctggaattc tttgcttaat aagcaaatgt ggaagatcta tttgaaaaat ggccacttcg
     6061 gaatgctctc atacgcttct ccagctgtct cgacttgctt tgaaaaaata cccagcgcct
     6121 acattgagcc cgcccaatac gactgccttc gcgatgaggg gtttgaatat gccaggaagc
     6181 ttgaagatca cggcgtcagc gtcgaactca attatgtgga gggcgccttt catgcctttg
     6241 atatcatgca gagcaagaaa ctcgtcagaa aagtgatggc ccagcgcatc tcggctttaa
     6301 gccgcgccct ccacccatca aaatgattta cgaattagca gcaatcacgt atagtatttt
     6361 caagtcgtgg taacacgact gttaacaaag atgaaaaaga aaattaatgg catcaatcaa
     6421 gactggtacc gccttgataa cgccgcgaaa atttatccgg ccatggtcag ccgccgcaac
     6481 agctgtgttt ttcgctttgc gataaatctt aaagaagaag tcgatcccta cgtcttgcag
     6541 cgggcgctcc tcgatatgcg gaatcgcttt ccggcgatgt ttgtccgcgt ccgacacggc
     6601 gtcttctggt attatctcga agagaacaag aaaatgcccc gtgtccgtcc ggaggatagc
     6661 cgcattaata aagccattga ttactatagt aataattatt tccgcttcac cgttttttac
     6721 tatgagaacc gcatcagttt agaggtcttt cacagtttga cagacggaac cggcgccgtc
     6781 actttcttaa aagcccttgc ttatcgctat ctcgagctca agggtcacaa actggaaagc
     6841 gaaggcctgg tgttgaccgt cgaccaagat ccttcaattg aagaacttga agacagcttc
     6901 tttaaatatg cgacgatgaa tccgcacaag cgcttcgtcg tgccgacacc ttttagcatc
     6961 cgcgatgccc gctttccgag cggtcagggt cgcggagtga tcaacgctcg gcttgattcg
     7021 gccgccttat atgcgcttgc caaaaagcac gatgcgacag tcaccgaata tctttcggct
     7081 ctgctgactc aggccgtcta tcaggtatat ggcgacaaaa tgataaaaaa tgaccgcgtc
     7141 aacatttgca ttcccatcaa tctacggcga ttatttcctt cggattccct gcgcaatttc
     7201 actcttttct ttcacacgac cgtgacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnaacg atatcaactg cgcctatggc atcggcgtca tcacctttaa taatgtcatg
     7381 acgatcagca ttcttcgcga agtgcgcgat acgttgctgc cgatgcagtt tttacgaaat
     7441 cttgtcaatt taggtttagc cgtcgaagtt gagagcaatt attgggagga ggtcgcctag
     7501 tatggattat tgtccggaat gtaaagtcca agtcgtctcc aaaggcgaga agtgccctct
     7561 ttgccaaggc ccgcttgacc gcggagagaa gccggcgtca aagaaacctt ttccttcata
     7621 tatcaagttt gtcaatctgc gcaagaggtg ggcccgcggc atttcgctgg ccgctccggt
     7681 cgccatcggc atctgtgttt tgattaacct tttgacatgg aatggttttc tgtggtcttt
     7741 aatcgtcgga gtggcaattt tatatgcctg gctcgtcggt ctatatacgg tgcgcaggac
     7801 cgatcacctc ggtcagaaat tgcttttgca cgcgattggc atctccgccc ttttaatcgt
     7861 cattaatttt gtgacggcct cgccctcgat gacctcttcg gaattccttg aaaacttctc
     7921 ggaaatcgcc cggtggtcgc ttgattatgc gatgccggcg acattgatgg ctttcatttt
     7981 ggcgataaat atcgccacgc tttcgcaaag gcaccgtctg cgcgactata tcatcgcgca
     8041 attttcttta tcttttatcg gctttatccc ctttgtcctg atcatgattg atatctttgc
     8101 cgccgatatt ttaggctctc ttttcatgtc gatcgccgcc tcttctttct cacttctaac
     8161 cctgcttgtt tttgtcatct tcgcgcctaa gctggttgca aaggagtttg gaagaaggtt
     8221 ccatatctag tagaataaga atatgaaata taatcacccg gaaaccgcgc ttgtaactat
     8281 catcggcccc gatcagacgt cgtggatagg cgacatcaaa gtcgaagttc ttttcgacgg
     8341 tgaaatcgtc ggcgtcatca aaggccaaaa atcggtgcag tttgaaatta acgaggacac
     8401 ccgtttttta atcaggacgg cgaatgaaga tttgccaaaa tccgaagtga ccgtcgccgg
     8461 actgtttcgc tgcccgacca cgattaaact atatttactc ggcagttcac cggcaaacgc
     8521 caaactggtt gctaaaacta catacggaga aaattattcg aaaaggaaat atttatgaaa
     8581 aaaatcatct tattcttacc tattttagcc ttgttcttgg cgggttgcga cggcggagat
     8641 tcaaactctt cgacgagcgg tacgtcagac gatagttcga cnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nccgctgaac cgcttccccc tcctgtcgtt tctgacatca
     8821 acggcgaaga gataagccat gaagcaaacg acggcgatat cattaaacta atgcacatga
     8881 acgacacaca tggctcgatt gaatatctgc ctgacaataa tgaaccgggc atggcataca
     8941 tcgccggtta tgttaatgaa aagcgcgctc aagaaaatac ggatgtcgtg cttttgtctt
     9001 cgggcgatat gtttcagggc agtctcgatt ccaatatcaa tcagggaaag ctgatgattg
     9061 atattatgaa agagatgcgc ttcgacagta tgtcgattgg caaccacgaa ttcgactggg
     9121 gcgtcgatgt cctcgcggag aatgcgcaat atgctctcga ttccgatacg ggcgactggg
     9181 ctttcccctt tttatccggc aacattttgg gacccgaggg gcaatatgaa ttcggtcatc
     9241 tttccacaac ctttaatcgc ggcccggccc gcatcagcat catcggttcc atcgatagcg
     9301 gcgtctacga ttcaattgat ggtccgattg tcgccggtta cgaattcgtc aacgccgctt
     9361 cgatggtcat ttctgaagct caaagattaa gggcttcggg atcagacatt atcatctatt
     9421 cgacccacga cgtcgataag cgagtcgcac agacaatagc caataatgtc gacgccatct
     9481 ttactggtca taaccaccat aattcagttt tgaccatgac tagttcgact tcaaagacga
     9541 ttccgattat cgaatcttcc tcaaatgggc gcgcgcttgg cgaggtcgat tttatctacg
     9601 atgccgagac cgattcatac gaactaggaa cctacaaaaa caccaatgtt ctcgatggcg
     9661 ttatttccct tattgaggat gacggcgttc aggcaattta tgatcgctat cttgatgctc
     9721 ccgcgcagga tggtccggtt tccagcgata gtctccgctc tttaaagagc gataaagtcg
     9781 gcgaaatcac gagtgactcg aagtatgtcg cctatgataa tgtaagcatt ccgaaatcag
     9841 atgtggcgac gatgtttctt tatgcccagc ttgaggcgta catcgattct tacgatattg
     9901 tcggatcttg ctacaacgaa tcccgctcgg catggattgt cggcaatatc acctatgccg
     9961 acatttataa agccttcccc tttgataacg ccaccgtcat tgttgcggcg acgggggcac
    10021 aattaaaaac gtggagtacc acaaccgttt atgtcgatgg aatttcaaag acgaccctag
    10081 ataacaatac tgtttatcaa atcgttacct caacatacat catcaacaac cgagaagaag
    10141 gcaaatttaa cagtatcgtc aacatcgatg aagatctttt tcaacgccac gtcttctatc
    10201 agcaatttct tgaggcgaca acacccaatc cctggggtta gtgttataaa acaggtattt
    10261 cttgttcatt tagtatattt gtgccacgat aattgccaag aggtaagcat atggtaaaac
    10321 gcatttttgg catcattttt gccctcgccg gtcttgcggc gatggtcgtc gccgtcatct
    10381 ttggcattca ggccgttcaa gaattgtcgg cttatattgc cggaggagtt gatccgctcg
    10441 ccccgattgc atttgtcgtc ttcatcgtca tggtcgccgt ttgggtcgcc cttttcatca
    10501 tcggcgtcgt cgtctttatt ccaagcctga tcgtcgccat caaaggcccc aagaagaaaa
    10561 aaccgatttc cggctgatga tgccggtttt ccttccgcgt tttattcggc tataataata
    10621 ttgatgaacg aaaataagct aaccgaaaag aagagcgaag aaaatagtca aacaaagaaa
    10681 aaaggccggt ggatttctta tctgcccgcc ttttttatag cccttattac catcgcttta
    10741 ttactgctta ttatcatcgg aacaatcttc tcggggggcg agaatcagga cgcgcaagtg
    10801 cgcgccctcc acagcatcaa ctttacgggc gagaccgccg agttcaacga tgattatatc
    10861 tatgaacccg cggtcagcga agagcccgtt tttgtcgccg aacaaggctt tcgctgggat
    10921 agtctcgcgt caaagatcgt ggtgcgcgtt cctgaatatc gggaagccgt atcaatcaac
    10981 ataggttttg gcttgttcaa tttcaaggct tattcggaag tcgaaaccag cagcgataat
    11041 tacgcaattc aggcttttga cggcgacggt gctcttttgg agacaaaaat gctcactaac
    11101 gtccaaaacg acagcgagca gcaagtcgtc tttcaactcg aagatgtcaa gcgcctgaca
    11161 atcacttttc aagagccgat aacaaacatc ggtgaagagg ttacgagcgg ttatctttat
    11221 ttaaaagatt tctccacgtg cgaaatcatc aattacgaat aataaaaata gaaatacagt
    11281 ttctgataag gcgagtactc tttaatgtaa tatactggat ctcctgtatc gggatccgtc
    11341 gtctcatcga tgtcgggtgt atcgacatat gaagagacgc cattatttcc gacgatcatc
    11401 aaatataagt tgttgaacca agtggccata tcatcgagaa aatcatattt ttcccaataa
    11461 gaacgcgagt attgataatc gcccttaatt tcatctctta aagaatttga taactcgccg
    11521 tatttatctt cgtcgccaac atagttcgat agagaaataa tgctgtagaa agttgtgaag
    11581 tatccggaat aacgcagata cggatcgtca ctattaagca ggatgtaggc cgcgaccaaa
    11641 tttgcatcct gttcgcgcat cgcgcctttg gaatgggcga tttcatgggc catcgttgag
    11701 acgagctgtg aatcgggagt caggtagttg atgtttggct cggtcgtcgg cgcaaaggaa
    11761 atgccggtaa tattaaactc gcggaataaa aaggacaaga acatcggttt aacgtttggt
    11821 gtaaaactgg tgaagtaatc ggattcaaga cgagcgtact caattttgag aatctcgctt
    11881 aattcgcgaa tcgaataaga gcggataaca ctgccttccg cggtgaactc gagttcgtcg
    11941 gcacaggcat taaagtcggc aagaaagtgt tcgataattt cgacatattc gctgctctcg
    12001 acatcttcct ggtgctgcgg aatgtcgatg ggatagcggc cataggcgac gccggcagtc
    12061 gcaaagtaat aagcgaaagt cgatgtcgca accagaatca aagctaatgt cttattgagc
    12121 ccgatcaggc ccctcttttt gctgaaatcg atgataatca ggacgataag ggtaatgatt
    12181 gtcacgccaa aaagaatgaa aaaaacctca gcgagcgaaa aaggtataag gctcgcaagc
    12241 gggccaaaaa atgattggat aaagcgataa aatgtcctgg tccaaaactc ggctgaagct
    12301 gtgaaaaggc gtatgacgat taaggccgca aaaagcaaaa taacagcaat taagacataa
    12361 atctttgcaa aaacggcttt tttaagctta tcttttccgt tcatgtgctt attatataat
    12421 ggactagtta caattgctat tttctcgttt tcttactgct tttcgcgttc ttatcttggc
    12481 gtgacatcaa gtcgcgcacc gtgtcttcga tcgtgttaaa aataggccgg acggtaaaac
    12541 ctagttcgcg aacggcaaga gcatttgaga agtttgaatt agagacgaga gtatcaactg
    12601 agtaagcagt gaaaataggg ggctgctttt tcatctttga ccaaagggca gccaaaggag
    12661 caaacatttt tacaaaccag atggggagga cgctacggat ttttcttctt tgcgagatgc
    12721 ggctggcaat ttcgatgatt tcatagatag tatgatattg tccggagagg agataacagc
    12781 ggccttcgac tccacgatcg gtggctttga taatgccgtc agctacatcg cggacatcga
    12841 cgatgttata tcctcctttg acgatggaag tcagcgagcc atcgaggtag ttttttaaaa
    12901 ccgcggtaaa atgcccgttc atctcgtcgt ttggaccgat gattccggac ggatgaacga
    12961 tgatgccgcg gaggccattt ttaacggcgt ctaaaacata ttgactggcc tcggctttag
    13021 ttttggcgta aaaaccgttg acctcatttg gatcgaagtg atcttgttcg gttattaggt
    13081 cctttttgga cagttcctta agggcgtgga cgcttgaaac atagataaac ttttcgacgt
    13141 tgcgtttttt ggcgctgtcg gtgatgcgtt ttgttccgtt gacattcacg tcataaacga
    13201 gttgattaaa cgagttctcg accgtgataa ttccggcgca gtgaatgaca atctgagcgg
    13261 cgtcctcgga tttgaatgac agaaattttt cgacatcttc atctttgcgg atatcgcctt
    13321 cgacgaaata cagttcgccc tgattttggt tttcaaagtc gcgcttctga tcgtgcggaa
    13381 gaataaaagc cacgatggtt ttcttttgcg aaagcaaaag attgacgatt gtgcgtccga
    13441 ggtgaccggt cgccccggtg atgtagtaga ttattttttt catgtttgca cctccttctt
    13501 tattatgtaa taaaaaagaa taatataata tacaattggt atcatttgcg cgaagtaatt
    13561 tcattgttat taacgagcgg gagcgaggtg tgataaaatt atctcatggt taaaaaagct
    13621 atttatcccg gttcattcga ccccgtgtcg aatggccaca ttgacatcat cgaacgcatc
    13681 tcgcgccttt tcgaggcggt ttatgtggtg gtggcagtca atccgaataa acgctatatc
    13741 ttcactgctg acgaacgcgt tgagatgctg aaaaaagcga ccgcccacct tcataatgtc
    13801 gtcgtcgaaa aaagcgactt aatcctcgtt gactatgtcc ggcttaaagg cgaaggcgtc
    13861 atcattcgcg gcgttagaaa cgtcaacgac tatcaaaacg aaatcacgat gtatcagttt
    13921 aatcacacca gcgatccgaa catcgaaacg ctgattatgt ttccgtcgag ccacaatctc
    13981 ttcatctcgg cttcctatat aaaggaactg gcccttttca acgccgacat ttcgccgtat
    14041 gtcccaaaag ggctcgccga ctttatttcg cgaacaattc gcgcaagaaa atagcattta
    14101 tcagcaattg ggaatttttc aggcattctt tcagcatttt ctcctcagtg acttatttat
    14161 aaaatggagg tgattatatg tttatctttc gtccatcact aaaaattttt aaacgtactc
    14221 ttgatatcta cgaagagaag cgcatcaaga agattaaacc tttcgccgag atgcgaggct
    14281 ggaaggatgt tccttacatc gatgacggaa gcgttgaaca cctcctcgat atttatcatc
    14341 ccgctgaaaa cggtaacaac cacttgatat ttgacattca tggcggctct tatgcttttg
    14401 gttttaaggg ccttaattac atttttaact cttactttgt cgcgaaaggc ttttccgttg
    14461 tggcaatcaa ctataccctc gtcaaaaaag gaacgagcat ctataatcag attctcgata
    14521 ttttcgccgc tttgcatttt ttggagaaga acaaagaaaa gtacaatttg tcatttgata
    14581 attttattct tctcggcgat tcggccggcg gccatctagc ccttcttgcc gatctcgttt
    14641 tcaagtctcc ggaagcgcag gaatactaca agataaaaag cctcccgaac atcaagatta
    14701 aatgtgtggc tatgaactgt gccgtctacg attttggcga ggtcgtcgat ttcggcaagc
    14761 gcgtgattca taaatcagcg atgccttatc ttttttctag caaatatctc gacggtgatt
    14821 ttctgaagat gaacaacccg gcttattata taagaaaagt caaacctgat cccatcttta
    14881 tctcttcatg tcgcaatgac tttcttaaga aacacagttt tatgctccgc gacgaactgc
    14941 tcagacaggg gctaatccat cactacgaat tcgaaccaac tctcgatgtc aaggtggccc
    15001 atgtctataa cctcgtcctg cccgacagca agcaaggcaa gcgcaccaat gatgcgatgg
    15061 ccgacttctt tctccgcgat ttcactaagt agatgctttt ctataatttt cattgaaaat
    15121 tacttttggt tgcgcgcata gcgaagataa tataagattt atgtgctatg aaattaaacc
    15181 agaatcaaac gccgtttctc gatgctctta ttaaatatgt cagtgaaaat atctcccctt
    15241 ttgatgtccc tggacatcat atgggtaatg tcgaaaaccg cttaaaggat tttttaggtg
    15301 cgaaaacttt tcgcagtgat gtcaatgccc ccatcggcct cgactccctt cagcatccca
    15361 ccggcgtcat cgcagaggct caggaactga tggctgattt tgccggcgct gatcacagtt
    15421 tctttttagt taacggcacg acgagcggca tcatcgctat gattatggcg gtgtgcaaag
    15481 ccaaagacaa aatcatcctg ccccgcaatg tccacaaatc gattatcagc gctttagttt
    15541 tgtcgggctc gattccggta tatgtcatgc cgaaaatcga tcgccaatta gaaatcgcca
    15601 atcagccaag cgccgatgac tacaagcggg ccatcatccg ctatccgagc gccaaagcca
    15661 tcctcgttat caatccgaca tattttggag ctgtcaatga cctcaaggaa ctaactgctt
    15721 ttgcccatgc ccacaacgtg accgttctcg tcgacgaagc gcatggcgca cactattact
    15781 ttagtaaaaa cggtcctttg tcagcgatgg cctgcggagc agatctttcc agcgtctcct
    15841 ttcacaaaac cggtggttcg ctgacgcaat caagcgtcct cctcttgcaa ggcaaacgcg
    15901 tttcgccaag cgaagtgcag aagtcgctga gcatcatcaa cacgacatcg ccgtctaatc
    15961 ttctcatcgc cagcatcgac gccgcgcgcc actttgccgc gaccaaaggt caggaagcga
    16021 tggatcaggt tctcgaactg gcgcagcatg cccgcgagca aatatcaaaa ataaaaggat
    16081 tcattccgcg cggcagagaa cattttttgc agaagggctg ctatgactac gacgagacaa
    16141 agctcgtcat cgaactcgat catctcgatt tgagcggctt tgatctttat tatctcctta
    16201 aggagaaata tcggattcag gtcgaactgg ccgaaactta tgtcatttta ggaatcttag
    16261 ctatcggaac aaaaaaagaa catctaaagc atctttttgc ggccctaaaa gaaatcagcc
    16321 gcgatcatta taatcgcaag attacctacc cccgccactc cttcagcgtg ggtttcccct
    16381 tcctgctcgt tcgccctcgc tcggcgtttt atgcaccgag caaacgaata tcgataatgg
    16441 atgccgccaa cgagatatcc aaagaatcta tcatgattta tcctcctggc attccgctta
    16501 ttattcccgg cgaaattttt accaacgacc tgattgaacg catcaaaagt tataaacaga
    16561 ccggtatcac gatgatcagc gattatagcg atggaactgt caatgtcgtc gacaaagaac
    16621 actggaagcg cttctcatca tatcaaaagg cttatcaaga ttattcatct aaacgcatca
    16681 cgaccccgca caacgatggt tattcaatgc cctttgaagg cgatgcccat caagctacct
    16741 ttatgctcct gccctttcgc aaagacacat ggcgggaggg agcgaaaccg gctcgcgagg
    16801 cttttaaaga tgtcattcgg gccatcgctc agtttgaacc ggtcatcgtc ggcattcacc
    16861 ctagcatata tgatgtcgct tcgggggaat tttcaaacat cgataatgtc accgtcataa
    16921 aaataaaata caacgatgcc tgggctcgcg ataatgctcc gatttttgta aaaaaaggta
    16981 ccaagattcg ctctgtcgat tttcgtttta atgcttggag cggcgaagac ggcgggctct
    17041 attctaacta ttacgatgat gatcgactag ccggcgttct ttccaaaaaa ctaaatatta
    17101 acagctatta catcgaagat tttgtcttgg aaggcggcag cattcatgtc gatggccaag
    17161 gcacctgcct tgttaccgag gcctgcctgc tttctaaagg tcgcaatccg catttaaata
    17221 ggcaggagat tgaagagacg ctgaaaacaa atctcggcgt cagcaaagtc atctggatca
    17281 aaaacggaat ctatcaagat gagacgagcg agcatgtcga caacatggct tgttttgttc
    17341 gtcccggtgt cattgccttg gcgtggacga cggaccgcaa agaccctcag tacaagtatt
    17401 cacaggcagc ttacaaagta ttaaaaagcg aaactgacgc cgatggcaaa cctttcgaaa
    17461 ttatcaaggt gcgtttgcct catccgatgt acatgacgcg cgaagaagcc aaaggcatcc
    17521 gcggctcgcg ttcgaatgcc aaaaagcgcg aaccaaacat gcgtctggcc gcctcataca
    17581 tcaattacta ccaaggcaaa gacttcgtta ttttgccggc cttcggagtt aaggaagata
    17641 agctggccta tgagcaattt tcctctcttt atcccgacaa aaaaatcatg caagtgaatt
    17701 ctcgcgagat ccttctcggc ggaggcaata tccactgcat tacgatgcaa attccggaaa
    17761 ccaaaaaagg agaacaacaa ttatgaaagt agctattacc caattcaaaa tgtcgtggaa
    17821 tattgatgaa aatcttgcaa aagccgaaca aatggtgcga aaagccgctc aaaaaggcgc
    17881 acaagttatc ctcctgcccg aactttttaa aacgccatat ttctgccaaa gggaaaatta
    17941 cgaatacttc gatttagcag aagaactcga ttcgtcgacg acaatttctc gttttcaaaa
    18001 actggcaaaa gaattaaatg tcgtcttacc tctttctttt tttgagaagg ccggcaacgt
    18061 gcactttaat tcactggtga tgattgacgc cgatggcaaa aacctcggct tatatcgcaa
    18121 atcgcacatc ccgactggtg aatgctacga ggaaaagttt tatttttctc caggcgatac
    18181 cggatttaaa gtatttaaaa caaaatatgg acgagtcggc gtcggcatct gctgggatca
    18241 gtggtttccc gaagtggccc gcagcctcgc cttaatgggc gcccagctca tcgtctatcc
    18301 gacagcgatc ggcaccgaac cggtcttacc gaaagattct aaaaatcact ggcaaaatac
    18361 aatgcgtgga catgctgccg ccaacatcgt ccccgtcttg gcatcaaacc gcatcggaac
    18421 tgaaagcgac aaagaatcga gcatgacctt tttcggcagc agcttcatcg ctggcgaaga
    18481 cggcaatacc gtcattgaat tatcgcgaaa cgaagaagac gttgcggtgg ctgaatttga
    18541 tttggcggag attgataaaa agcgcatcgc gtggggcgtc tttcgcgatc ggagacccga
    18601 cctctatcac agcatcacca aatttgatga ttccaggaaa tgaacttatg ggtttacatg
    18661 cttttcgcgc tggcgcgaat gccttatgct atgtaaaaaa tgaccataaa tacggcatga
    18721 tttgcgcatg ggccatgatg atagattatg atgttatcgc tctgttggta ggcgagcaaa
    18781 gcgcgaccgg caagaacctt gaagtaggca tgctcgtcgg cgtcagtgct ctcagcaaag
    18841 aacaaaaagc cgtggccctg acattaggca acggacacag tgatgaaatt gataagtttg
    18901 ctaatttatc ctatgaaatc gatgagagcg caatcctgat cagaggtgcc agaaacaatc
    18961 ttgtcgtcag agtggaaaaa ctcattaaac tcaatcctga aaatcaagat attctcgtta
    19021 tcgccaaatt tgcgaaaagc aaagaagata agtcaaaaga atttatcgac gccgaagaag
    19081 tgtttcgtta agattatttt tctttgacga gattttcaat tttcttaaaa gtttcctcag
    19141 ataaaacatg ctcgatcagg cagcagtcgc gatcggcatt tttttctgaa acacccaggc
    19201 tgagcaaata tttcttaagc gtcgtgtggc ggtgatacac ctttttcgca atccgttttc
    19261 cggatggcgt aaactcaatt tccgaatatg gagtcttgtt gataaaaccg aggcgcatga
    19321 gttcatgagt ggctttgttg acggctggac gcgagacatt tagtagacgt gaaacgcggg
    19381 cagattctaa aggttcttta ttattataaa gaatatagaa agcttctaaa taatcttcaa
    19441 ttgctttgct cgtttttttc atcggtgttt tctcttgtaa acgctatcat tatatcactc
    19501 tcgttttgcg aatacaaaaa agttaaccat ggtttacaat ttcggttgac ttgccatatt
    19561 cttcaactta atatatatga gagcaaactg aggtggcaaa cagaacgata tgcaagaaag
    19621 aaatcgacat caacatggga aaagacaacg tctccagacc ggacaggagg gtcatttaag
    19681 cgacctcgac cgcggtcaaa aagcgattgt tctcgatttt cataacgata atgccgcctt
    19741 gcgtcgccgc cttcttgata tgggcatcac caaaggcgtg gaaatcgaaa tcaaaaaaat
    19801 cgccccactc ggtgatcccg tcgacattcg cctgcgcgga tatgagcttt gcatacgtcg
    19861 caaagatatg cgttcaatcg atattaaggt cgtgaaataa ctatgaaaat cgctttagtc
    19921 ggaaatcaaa actccggcaa aacaactttg tttaacaccc tgacgggaac gaatcaaaaa
    19981 gtcggcaact ggccaggcgt caccatcgag cgtaaagaag gcaaaattag cggcacggaa
    20041 atcgaaattg ttgatttacc gggtgtctat tccctatccc catattcgac ggaagaagag
    20101 atatcacggc agtttgccat ggaagaaaaa cccgatttaa tcattaatat tatcgatgcc
    20161 accagcctcg aaagaagtct ctatctaaca actcagctcc tcgaattgga tgttgatgtc
    20221 atcatcgcgt taaacatggc tgatgttctt gaaaaaagcg gtataaccgt tgacatagag
    20281 tgtctttcac ggcgcctgaa tacaacgatt atctcaatct ctgccaaaaa gaaactcgga
    20341 gttgacaatc tgatttccgt tattaaaaac ggagaatata agaagaatca ccatgactat
    20401 atatacgatg aagaaattga gaatgtgata tcaggtataa catcgcaaat caaggtcggt
    20461 cacaagcgct tctcagcagt aaaaattatc gagcgtgaca gagattatcg ccctttgctg
    20521 accgatgaga ttaccgcaaa aatcaacgct cttgaagaaa tgtataaaat ggacggtgaa
    20581 caactgatcg ccgatcagcg ctatcgccac attgtccgca ttaaaaaaga atgttcaact
    20641 cacaccgaaa agggtgaaac aatcaccgat aaactcgacc gcgtcttttt acataaatat
    20701 ttcggtctgc ctatttttgc tttgataatg ggtattgtct atttcttatc tgtcggtctc
    20761 gtcgacgggc tcaccgtcga ttttgtcgca gccctttttg atggtgctga aacaatcgat
    20821 gtcttgggca cggaagtccc gtttgcaatc gccggtctgg gtccaacgct aagcgcctgg
    20881 ctgacgaatg tcggagccag tccttggtcg gtatccctcg tcgttgacgg cattattgcc
    20941 ggctttgggg ccgtgagcag ctttcttccg caactaatca tcctttttct ttgcttagcg
    21001 cttttagaga caaccggcta catgtcgcgt atctctttct ttctcgaccg tattttcaag
    21061 cgttttgggc tgagcggaaa atcactcatt ccttttattg tcggaagcgg ttgcagcgtc
    21121 cccggaatca tggcctcaag aaccgtagaa accgaagagg aaagaaaaat gactgtcatg
    21181 ttgacgccat ttgttccgtg ctcggcgaag ctccccatca ttgcactatt tgccggctat
    21241 ttcttttcga cttatgcctg gcttgttacc ctcagtctct atttcatggc gataatcgtt
    21301 attctgctat cagcgctcat tttaaaaaag ttcgtctaca aaggcgcgac ttcatcatat
    21361 ttatcggaac tccccaacta tcatgcgccg agcgccttat atgtcttccg cgacgttttt
    21421 gataagactt ggtcatttat caagagggcg ggaacagtta tcgttttatg ttcggtcgcg
    21481 gtatggtttt tagcttcatt cacctggaca ttcaaatata tcgatggcgt caattttcat
    21541 attgaaaata gcattttagc aggaatcggc aatgcttttg cctggatttt ttatccgatg
    21601 ttgggtggga cgtggtcatg gggcgcgacc gtttcggcga ttcaaggact aatcgcaaaa
    21661 gaacaagtcg tcagttctct tgaagttatc gctggtcttg aaggtggcgg cgcttcggtt
    21721 tttgaaagcg gaatcttttc tttcttcacc ggatggagtg cctatgccta tatgattttt
    21781 aatcttttca gcgcgccgtg ttttgctgct ataggtgcca tgcgcaaaga gctcggatca
    21841 acaaaaggca tgcttaaagc cgtcttattt caaactggca ttgcctggct tatcgccact
    21901 tttgtcggca tcatcggatg gttgataata tgacatgggt agacggagtt atcttaggca
    21961 tcgtcgtcgt cttgctcggc ttgatcatct atttcagctt aatcaaaaac cgcgaaaaag
    22021 gcagttgccg aaattgcccc gaagccaatg gcgtcaaggc caaccgtttg ctgaaagatt
    22081 ataagcgcaa gtttaaaaag aaatagagag atggagatat tctccatttt tctttatgaa
    22141 ctagttgata tacaattaat tgagcatatg aatattgaaa aagttgccct agtcttacaa
    22201 ggcggtgccg ttcgcggagc ttttacggcc ggcgtccttg atgtcttgat ggaaaatgat
    22261 ctttattttc aatatgtcgt cggcgtctcg gcgggtgcgc ttatatcgtt aagttatctt
    22321 tccaagcaaa ttggccgctc gcggtcggtc gtcgtcgatt tcatggccga taagaaattc
    22381 gtcaacccca gatttctttt gacaaagcga tcgttattcg actttgatta cttgtttggc
    22441 gacattgcca cgaaaatact gccctttgat tttgatacct atgagaagag cccgattaag
    22501 tttgtcgtcg gaaccacctc atgccatagc ggtcagcctc tttattttat aaaagatgag
    22561 acgcctgatt tttttaaagc gattgccgcc tcatcgtcgc tgccgttttt cagcgccccg
    22621 gtcttcgtcg gcgaggaacc ttgcctcgat ggcggcatcg ccacccccat cccattccaa
    22681 aaagctctcg atgatggtta taaaaaaatg attgtcatca cgacccgtga caaaaactat
    22741 cgcaaagatc ttgacaaacc acaacacaaa aacggcttaa aagctctata tggcaaatat
    22801 ccgaaattca tcgaggccgc ccttgaatat cctcggcgat ataatcagga tgccgcccgc
    22861 cttgaggaac tagaaaatct cggcatggct tttgttgttc gtccgcggct gccgatcact
    22921 ttgaaacgga ccgagaaaga tcgagcggaa ctcgctaaag tctacgagcg gggaatagct
    22981 gtcgccaccg aactccttcc aaaaataaag gaatacatca aaaatgaata aaaatgataa
    23041 gagccgccca atgacgcgaa gccgtgaagt agatccgaaa aacattaagg agttcgccgt
    23101 caaagaaccg tgcgaactgc tcactttttt gctcgaaaag ttgccttctt tatcccgtaa
    23161 taatgtcaag cgcctcctcg ccaataacca agttgccgtc gatggcgcgc ccattcgtca
    23221 attcaacttt ttactggcca aagaagatgt tgtcatcgtt tccaaaaacc gcattacttc
    23281 gaaaaaaaga gaaaatcttc cgattattta cgaggacgag gagtttatcg ttatcgataa
    23341 gccctcaggc cttttaagcg tcgccagcga caaggtcaaa gggcggaccg cctaccgcat
    23401 gcttatggat tatgttcagc agaaagaccg gcacaatcgc atctatgtcg tccatcgtct
    23461 tgacgaggac acatcgggcg tgatgatggt ggcgaaaaat gtcgaaattc gcgacgccct
    23521 tcaaaaaaac tgggccagca ttgtcacatc tcgcggatac tatgcgattg ttgaaggcgc
    23581 gatggaaaag aaagaagata ctctccatca ttatttggcc gagaataatt taaatcttat
    23641 gtatgtgacg acaaataaaa aaattggcaa ggaatgcacc acccgctata agacgatttc
    23701 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatatata tttatgtctt
    23821 ttagttggct taacaaattt aaagcggctt tgatttcgac tcttccgatg gtcgtcatcg
    23881 ttttaattat ccactttacc ggtctcgcta aaatcgatga ttcaaaagat gtcataatct
    23941 ttgtgatttc agctgttctg gtggctcttg gcatgggcct tttcacgatt ggcactgatt
    24001 tggcgatgtc gccgattggt gaatatatcg gcgcgtcaat caccagccaa aggaaaatcg
    24061 gattaatggt ttttttgttc ttcttgctcg gcgcttttgt cgtgattgcc gaacccgatt
    24121 taagagttct ggtcggacaa gttcccctcg gcgggcaatg gcttcctttt attagcaacg
    24181 aatggctaat catcatcttg atcggtgttg gttttggagc cttcatggtc gttggaattc
    24241 tccgtatcct ctttcaaaaa agcctcgtta tctggcttct tcttttctat ggcttactat
    24301 tctctctggc tctcggcgtc aattatgtaa acccgtcttt tattcccctt tcttttgaca
    24361 gcggcggagc ttcggcaggt acgattaccg ttccctttat cctcgctctc ggtatcggta
    24421 ttgcgacgac gaggtccggc aagaaaaccg acgaagactc tttcggcctg atcgcaatta
    24481 catcgcttgg cccggtcctc atcgtcatga tcatgtcttt ttttatcgaa gacatacctt
    24541 ttatttacga accggaagcc gctcaatctt ttggggcgat tctcaaaacg agcgcactta
    24601 atgtcgcgat tgccatcctt ccgattaccg ccttcttctt tatttaccaa gcgattgcca
    24661 tccgccttcc tcttaaagtc ttaatcagaa tcatttccgg cctcgtctat acttatttag
    24721 gcctcgtcat ttttttaacc gcagttaatc tcggcttctt gccgatcagc cgcatcatcg
    24781 gcagcaaaat cgccgcgcag agttcgagcc tcgtcattct ctttggcgcg attatcggca
    24841 tggccgcggt tcttcttgaa cccgctattc acgttttagt atcgcaagtt gaaaatatta
    24901 ccgaaggtac aattcgcaag ggtgccgtct tggccgcgat tgccgtcggg gcgggtctag
    24961 ccatgtcgtt tgcgatgatg cgactagtgt ttggcatcac gattttatgg tttgtcatcg
    25021 gcggttatct cgtcgctctc ggaatggctt ttttcgttcc caaaatctat tcggcgatgg
    25081 cttttgacgc cggtcgcatc acgagcggac cgatgaattc ttcattcgtt ttaccctttg
    25141 ccatcggcgc cgccatcacc ttttatcaaa gtgacggaca agatataatg acttatgcct
    25201 ttggcgtcaa tgcccttgtg acgatggttc ccatcatcac cattcaagct ctcggccttt
    25261 atgtcaccgc ccaagagaaa cgccgcattc gccttgccgg tatgcgcttt gtcgaagaaa
    25321 acgatgatca gattatttac ttactagagg atgagcaatg agcaagaaaa aagtagcgag
    25381 caacccctct cttccagttg aatccaaacc ggaaactttc aagtccgtcg accgcgtcca
    25441 acccctttcg cggctcaaat ttttcattac catcgtcaat cacaatcact ataaggtggt
    25501 cctgaaaatc ttaaaaaatc acgagagtgg actaggtttt atcacccacg gtcgaggaac
    25561 cggcaccaag gaaatttatg acctcttggg cttaagcgat gtccataagg acattatctt
    25621 cgccgttgtc aaagccgatc aaattgaaac gctaaaaaaa gaaatcggcg ccttctttgt
    25681 gaaagaaagc aaaggaatcg cctttgccat cgacatttca tcggtgattg gaagttcaat
    25741 atacaagtat ttgtcaaaca cgcgaccgac aggagagtaa ctatggaaaa acatgagctg
    25801 atcatctgca ttgtcaacaa cggcttcact gatttagtga tggacgccgc ccgcaaagaa
    25861 ggtgcgcgag gaggcactat ttttcatggt cgcggcaccg gcaatcccga tatggagaag
    25921 ttttttggat tttctatttc tcctgaaaaa gagattgtca ttatcctcgt cgaagaagcg
    25981 attaaagacg ctgttttaaa agccattaac aaagctgcag gcttagagac taacggcaaa
    26041 ggaattgcct tctcgcttcc cgtcagcgat accgtcggcg tcaattgaga ttagagaact
    26101 cgagtagcga ttccgggcct gaccttcggt ggcaggtcct tttctttggg gtgaccgatg
    26161 acgacggccg acatcgcccg atactcatct gaaagtccca gagcctgtaa gagaaaaggg
    26221 cggccattaa ttacttcgga aacggtgtaa aggaagcagg agccgagccc taggctcgtg
    26281 gccgccagca tcatattttc aatcaggcag ccggtatcgc agccatcgag gcgttcgcga
    26341 ttcggtttag cggcgacaaa gatgacgata ggggcgtgaa agaaagggtg tttagttaat
    26401 attcccgcct cagcaatctt ctgagctaag gcatcgattt tttctttatg tttgatgaca
    26461 atcaattcat agcgattgta ggcggcgcgg ccaatcggag catggtaggc ggcgtcaaga
    26521 ataattttta aatgttcttc agagattttt tcttcgctga agtcgcgaac tgactgacgt
    26581 tttttaattg tgtccagaat atccattttt gatctcctat tcttctttta tggtaacata
    26641 cattattgtg ttagaataat tttatggcgc gcggatggtt tcgctttaag caattcattt
    26701 ttaaaaaaat gccttatcta aaagaccggg gttcgctttt ttattatggt ctatttctag
    26761 cggccatcgg cctgggtttt tacggatatt ccttgctgac gcagtatttc agcgttccct
    26821 taggcggcga ctacactcag caatatgtcg cctttcatta taacttttac gacgactggt
    26881 ggacattttt taaaaccggt gacttcgtga tgtgggacca caacacattc ttgggcgtcg
    26941 ataacattac ctcaaacacg tattacggcc tcttctcgcc attcatgctg ccgatacttt
    27001 tgtttccaag gtattttata cctcatctct tggcggtgac gatgatcgcc cgcatggtta
    27061 ccggcgggct ccttttccgc ctctatctaa aacatttcaa tgtcaaagaa gggactgctc
    27121 gcctctttgc catcgcctat gccttttgtg gctggatcgc ctattacctg tggttcaaca
    27181 acttctttga agttattgct ttctttccgc tggtcttgct cggcatcgaa aaaatcttaa
    27241 atcgcgaaaa accctacacc ttgatcatcg gattatttct cattggtctc actcattatt
    27301 ttctgcttat caccatctgt ttttatggcg ttgcttatgc tctgtttcgc ttttttcaag
    27361 ttcgtaaaca gcacacgcgc aaggaaaact ggataatcct tggctggggt gtcgggtctt
    27421 ttgcggtcgg cttgatgcta gcggcctggg ttttaatacc aaattacttg aatgccatgc
    27481 aggcgccgcg cgccgaagat gccacctatc tcctc
//
LOCUS       DAOSSL010000011        26030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2075_length_26030_cov_15.981367, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000011 DAOSSL010000000
VERSION     DAOSSL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 26030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26030
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_26030_cov_15.981367"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..3009
                     /gene="ileS"
                     /locus_tag="PLH17_02090"
     CDS             271..3009
                     /gene="ileS"
                     /locus_tag="PLH17_02090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOR20607.1"
                     /translation="MELKKTLLMPKTSFEMRGNLNLKEPLFLARWQEQDLYQAMLKSR
                     VGAKEYALHDGPPYANGNIHCGHMLNRLLKDFIVRYKHMSGFYTPFVFGWDTHGLPIE
                     NQVTKQGVNRKATPIVEFRKKCREYALKQVEIQKEQIKRLGVVGDFENPYVTLDKQYE
                     ARQIEVFAEMALKGLIFKGLKPVYWSPSSESALAEAEIEYYDVTSPSIYVSFDVVDGK
                     GILNRGDKLVIWTTTPWTLPANLAISVHPRFEYGLYQTNKGRLILLKEFKNRLQKELD
                     LVEMDLLKTFNGQQLEGVLAKHPFYERSSLVLNGEHVTNEAGTGLVHTAPGHGVDDYN
                     VCQKYNILPYCPVDDRGFMTSEAGERLAGLFYEEANDVVLAMLRENNALLCEDKSLHS
                     YPHDWRTKKPLIFRATPQWFCSIEPIRESLMNEVKKIHWVPSWGETRMLNMIKDRTDW
                     CISRQRAWGVPLPIIYNEDMTPIIEREVFDHIIALFKEHGSDIWYEKEAVDLLPKGYR
                     NARSPNGLFSKEKDIMDVWFDSGSSFNGVMRERGLKFPADLYIEGNDQYRGWFNSSLT
                     ISTAVFGTSPFKDCVTHGFVVDENWDKMSKSKGNGIDPSKVANQFGADILRLWAATID
                     YKQDVRISEGIITQVAEVYRKVRNTFKFLLGNLANGEHSNFSPSSDKVEVFEAVDRFI
                     LAKLESVKNEAIGAFDKYDFGNGINRIVTFMSSDLSSFYLDITKDVLYCNSQKSPRRL
                     QVQNVIYQVTDTLMRLLTPFLPFTMDEIYHSLSYANKDNVQLLDYPRISHQFDESIIK
                     DYQALMELRDIVLKKLEEARSSGLIGSPQEAHVSLEENNALLKKLKLDENEAELARLF
                     VVSKVDLKAKDGVLITRAEGEKCPRCWNYVEQLHEHEEHAVCPRCLGVLKENHDGR"
     gene            2999..3649
                     /gene="lspA"
                     /locus_tag="PLH17_02095"
     CDS             2999..3649
                     /gene="lspA"
                     /locus_tag="PLH17_02095"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOR20608.1"
                     /translation="MAGKIANYFKSNSIKEIARDFFLSFVWLIVLLFVIDLVSKWVVV
                     NNMNVYTEITVIENFFYLRLSFNQGAAFGLGNTGDIEWRIFFIAVSAVIGTAMLVYYI
                     KKYRTLSIVMKIAVAMMIAGAYGNLIDRALYWPSTVGFSGVVDFLSFEFWGRRFATFN
                     VADMSLVVGVIILLFAIVIEDIRIAKNKEGKEDLSKPSAEYLADKEKKHGGEDEKL"
     gene            3624..4532
                     /locus_tag="PLH17_02100"
     CDS             3624..4532
                     /locus_tag="PLH17_02100"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010622168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR20609.1"
                     /translation="MAGKTKNYKVDREAGQRIDKALVEILIDVSRSHIQKLIDEGYVF
                     VNNEAVKTSYRLQKGDVINIVTREDQPSDLKPEYIPLDIVYEDSDIIIINKPRGLVVH
                     PGAGNKDLTLANALMFYTKNLSSINGEFRPGIVHRIDKDTSGLLAVAKNDYAHNELAA
                     QLKNHTMQRIYVALVHGVIHEEEGKIIAPLARDKKNRLKMAVDLQEGKEAVTHFKVLK
                     RYQTSTLVECRLETGRTHQIRVHFEYIRHPLVGDPLYGKKKDALYQGGQLLHAKALTL
                     IHPRTKEVKTFACALPAYFEEILKNL"
     gene            complement(4598..6643)
                     /locus_tag="PLH17_02105"
     CDS             complement(4598..6643)
                     /locus_tag="PLH17_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20610.1"
                     /translation="MKKSLLLLPLVAMSLAGCEPPIEELGDLLDIEVASGLKQEYVQY
                     TIVELDEISVTATFEKKTVTIEGSDLSFDPETLDTSTLGESEITITYLTESVVWTYTV
                     VEYDEIDNISAPDFVTTYLANIEAKDVANKRNEFMDREQGYFVGDDNPFKFFPNIYAY
                     DGDGIEMEVTAYHSVSVVEEKRGTEWVILTGAELDGVVTIDDFASTYDFSEEAIGNTY
                     RLTVRPFGDEYAVNPKFSTSFEFNVADGYNVYKQDDLSHFNNVGTHWDAYRTSKGITP
                     VAINGLFLHGDIEIERSKLPDSFFYMEGDSDINSGDGDYERALGSLRDNVDIYHHDVA
                     PGEAFVFNGNYFSIDYSTLPVVARESGRIDADVGLVISHATLIKAGYAQTGEDLGDYT
                     MRNLRVIGNANRTEAGAKSGGAIFTKLLSVDSHIYNLIGTQCFTFILTELSGPNAIIE
                     KTRGYDSFSSMLYNWGTDNLQIIDSEFIGAGGPIIIADHVSPEEDGTGGNPSHTTFTN
                     TVAESIVTGNENWFRLVHADAALPSILGFGEQVLPNYGTNSITQLININGTDIPHFNM
                     IGVVKDGGASEPSSTKISGSMTIDDGAALDFNGTFMTNSAPYPPAMPRFESSDGQQAL
                     FDGEKLVGLDNNPITPYVYSQTNYFTGPYLNMYVSIGASPTDYTAGYMGLVFGLQER"
     gene            complement(6732..7193)
                     /locus_tag="PLH17_02110"
     CDS             complement(6732..7193)
                     /locus_tag="PLH17_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20611.1"
                     /translation="MKKSVILIIGALSLVAIIVIGLLFQRAEVYNVTIYVSEIICSGV
                     RVGDDYYDTYFDESVNVYRIDNPDNPGSPLTLAYAPGLTVDIIYEVLPFEATNQSVSF
                     STDPNSFIARVESATGRVFFIDEGTETFTIRANDNSNKSAKVRLRAKIPDA"
     gene            complement(7196..8872)
                     /locus_tag="PLH17_02115"
     CDS             complement(7196..8872)
                     /locus_tag="PLH17_02115"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20612.1"
                     /translation="MNDIKTLIMMQLKDKLDLGFIKDKAQLIRKIVFFALRFIIVAGL
                     SYALFYVSSFLRIFHNSPFIPTSIMTMLLTIILVISTFASTNDLIKSLYMAEDNQVLV
                     TYPISANRIFLSKIIVFYLYEIYKNFTFTLPIFVAYGLISPVNWFFYIWVFLSFFVVS
                     MIPVVLGVVLSLPGFFIMRFFNRFRVLKILFFIAVLAGFIYLLVRIILLIPSEINVLN
                     YWGPIKVLLSDVTMFFQDYFKPVYYVVLMVVGKYNAAMRYTYLNLDVWLVFISLIGVL
                     AGLFMAAYFVARFLFIKMTSASFEYEKRFRVKALLNRRLPAFLSFVVKELRLIFRSGT
                     FTSNFIATYISIPLFILLINRIFASMDLYPNGQFAVQAFNILIIMLPLLASNSVVATM
                     YSREGRTAYMKRTKPVVVIFPLLAKLLPNIVLSIVSLCVSLYIFNAHMHYVLSNIILL
                     SIALIFIQVGHILFSALLDLMNPQNEQYATAGEQVSNSNETKATIFAFITAFLVALIT
                     LGFLVEEQFMPDQSFNYAFLKMLLIGFVFFGASVYMYIEKIKAYYYDRIS"
     gene            complement(8880..11093)
                     /locus_tag="PLH17_02120"
     CDS             complement(8880..11093)
                     /locus_tag="PLH17_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOR20613.1"
                     /translation="MTHYLSLIIALITASAGALTTSFLARNKNFSLGKKIFAFVLIAV
                     FFTRYISYDDQILNIVSLGAGPFSPVVNLFAYFGIWLELTLVVFLILYPFFKARILTN
                     LIKFVLTPGFVLYLGFSYYSVFLQVIGNTGGTLVLSFQSVMFALEIALVAYGLFLVWR
                     DDHTLKLDKKEILALLIAIVPVLVASLPIYGPQLMFGNANARYEVIDISFVHRLFIYA
                     TVIIPLVLYFSLKKKDPELIRLAMVYLSVVTMITFSRVFYYQNFLEPWTWPIHLCNTA
                     MYIIPLVLIFKLDKLFYFTYFINVFGALMAMLMPNYAETTNLTSWVIIQFWYNHSLAF
                     FMPLLLVALKLFPRPKMKQMYYSLIAFSGYFLLVMVLNVWFSNYSPTDFFFINSDFIV
                     DKLGRWAENIFNIAISFNIGDLVFEFHPVYQALFLIVYVGVSFAMWFVYSLGFSIADS
                     LGDLRFRQKKIKLDKYALLAALNGRGIDEPMEENTGVKLELKNFSKRYGKNKDFAVKD
                     ACLVVSGGEIFGFLGPNGAGKSTTIKSIVGIQTITEGSISICGYDVEKQPVMAKRLIG
                     YVPDHYALYEKLTAREYVNYIADIYGVTYEERQMRIPHYVSLFHLEDAFDNQIKTFSH
                     GMKQKVTIIAALVHEPKLWLLDEPLTGLDPDSIYQVKECMRQHAAKGNIVMFSSHIID
                     VVENLCQKIAVIKKGHLFAPLTIDEVHEQGTLEEYYLKMAIHEETPEQKATKEIA"
     gene            complement(11197..12531)
                     /locus_tag="PLH17_02125"
     CDS             complement(11197..12531)
                     /locus_tag="PLH17_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR20614.1"
                     /translation="MKRVFPFVLLDALLLTACEPRDSTLSDTSSNISVGSDIIGGSYE
                     ERISTMADTGIAIDALDKTEKEVASFDELAPLGSAAHPLSNSSAEYLEILTFEQPSQY
                     GDAYLFRAGEATIAMDFGHPSNYKYTDQTSYYSELKNTYETYIDDGHLDLLILTHPHG
                     DHYGGYQALKDAIDSVGMIVDYGYYSSGGENYRKDIRDYYADKGSAYHRIYDNVNEID
                     GGQKRTYITPDLFIDWLDTGYYTENYNDGPVVDDINVTSLAGILAYRDFTFFFSGDLQ
                     DNADGYNGGSGGLGETNLVAINGDNNEIFHEVTMMKAAHHSSSKANNDILLNTLEPKL
                     VTISAGAPSPDTSYGGKTGACAGHPHLQALSRFLTKGQKRVAPKIYLNFVNGTTSFVT
                     DGLNKVYMEGSPLKMSYYGTSTNGTVSQTYGSIEDIYNDILSSEWARVCHGL"
     gene            complement(12528..13457)
                     /gene="rnhC"
                     /locus_tag="PLH17_02130"
     CDS             complement(12528..13457)
                     /gene="rnhC"
                     /locus_tag="PLH17_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOR20615.1"
                     /translation="MGNNIVTLKANQELLEKIREYYRNDQILDAGQYVLFRAKKGKVV
                     ITAYQNSKGKDTKITFSGEGFIYEARRWDKTITISEDIKEDDETLGWFEIDDQIGSDE
                     VGTGDFLGPICVCAAYVKKQDVAYLKRIGVADSKKISDDFIKNITPLLLKKFSYSQVS
                     LTNEKYNELIKRGININEIKARLHNRVLLNLIKKHDVKNVYVDKFVSETRYFSYAARD
                     EEIVRDITFKTKGESRYPSVALASVIARYSFLKKMEALSKKYAAIIPFGASSKVDSFA
                     LAFIKKHGIEELRKIAKTDFKNYQRVTKNETEK"
     gene            13529..15835
                     /locus_tag="PLH17_02135"
     CDS             13529..15835
                     /locus_tag="PLH17_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOR20616.1"
                     /translation="MPTIYEVLEFGQIRKEIAAFAQSDIGRETSSNLVMLSKGELKNE
                     LAILDEMMVLNLRYGRLPMMSSTNLSQKVEYAFKGGTLTAEDLEAIAHDVIITGKIVL
                     SIRKREEKFPLLNDKIRQFKSLEQLEAAIHRVVGPDLGILNSASKKLASIRKSITETE
                     RKMTIRFQSVIESLRDYMTEAVATIRNGHFVLPIKTAHKSKVSGVIHDISDTGMTTFI
                     EPDWAVESNNAVYVLKIEEREEIQRLLKELSILVATHGHEVLANNHTIGYLDFVQAKA
                     LYAISLDGKIAELSEERAIDIKQARHPLINREVVVANDFSFDDSLRISVISGPNAGGK
                     TVALKTLGLMVLMNQCGLAIPTIKPAKMAYFNHIYADIGDNQSLASNLSTFSGHIKNL
                     AEIAALVDSDDLVLLDEIGTGTSPLEGEALAISYLEFLRDIGTFVFCSSHYDGLKDYA
                     LNKDGVTNASVLFDEQKMTPTYVLKQGVPGRSYGLEMAARYDLAKPIIDEAHNILKSH
                     GHEQSISALGNLQKLISENEIKAHLLENEVKRMQNFERTLQERQQKLDQQRQNLLEDV
                     SDAKQEILARAEKEIDEIFALLRNPDIKMHEVIEAKGKIRKLKEDETDVVLSENGDIE
                     VGSYVAITDLGITGKIEKISGNRLFIVSPDGLKITSTREKVHLVGAPAPEKAEKTFVA
                     PQIVSDVKLELNVIGLRVDEALEEVARYLDGVRLKHFSQVRIIHGFGTGALKRAIHDY
                     LSKCDFIKSYRPGDQHEGAGGATVVIMK"
     gene            15832..17586
                     /gene="uvrC"
                     /locus_tag="PLH17_02140"
     CDS             15832..17586
                     /gene="uvrC"
                     /locus_tag="PLH17_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOR20617.1"
                     /translation="MTEAVKQALLLVPTKPGVYLMKDKDEEIIYVGKAKNLSKRVTQY
                     FMRPQSGKVAAMVFNVDHFETIITKSEKEAFILEMNLIKKHYPRYNILLKDSKHYPYI
                     ALKRKNSPVLKIARDDKDKNYFYFGPYPTSMHAYEVISLLNKIFPLQKCKVLPPVPCL
                     YYHLGQCLAPCINKIDEETHVAIYNQIKSFLGGNNEDVKSQIRRKMEEASDNQEYEKA
                     GDYRKTLLAIDHINERQSMEVKDHVDRDIFAYVTRDGYLALALLTFRHGIMLGKEVFV
                     VEQFGEDIDLVTDLILQYYQTHSLPNEIVANIYGLKARIEDIYDINVVYKSRGALYDL
                     VDMATINARQGLDEHFMSARLTDDNLKILTELGNLLNIKTPTHIELFDNSHLQGSSPV
                     GAMVAFINGEPTKKMYRKFHIEGDEKRDDFASMKEIIYRRYKRIKDEDLKMPNLILVD
                     GGLPQLSAAYEALERLKLSIPAFGLYKNDRHQTEGLIDKAGLKYDLQNNKPLFFLLMR
                     MQDEVHRFAITFHHKLRSKAYKASVLDGIEGLGKRRIEILYRNYASINELKKATLEEL
                     QQLLPKNVASRVKERLEE"
     gene            17640..17716
                     /locus_tag="PLH17_02145"
     tRNA            17640..17716
                     /locus_tag="PLH17_02145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17674..17676,aa:Arg,seq:tct)
     gene            17867..18712
                     /locus_tag="PLH17_02150"
     CDS             17867..18712
                     /locus_tag="PLH17_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOR20618.1"
                     /translation="MSFEKNLNLIRKRNGMTQEDLALTIGVSRQTIYAWEAGLNSPNI
                     SMLKKVASALEVSIDELINGYDVDRLPNLLGEIVFSNESEYYEDVIYEEIPNWYVSLK
                     VGEDVCFGLYDNGVKDYSYHVTVLGEIIVHNQKGYEIGVKEYDKKLEQTDAFSLIAKR
                     VENRIIFIGRIYYKDGIKNIETFRDKSFLNKWGTDGKFEGPPIIFQEAKKYAMTYNGK
                     TESVIRIAYFDANNSYIEVFLNLKGESLFWRRYELGRKSGTILKINDKEYGLFYEVAT
                     SRLIR"
     gene            18800..19528
                     /gene="nagB"
                     /locus_tag="PLH17_02155"
     CDS             18800..19528
                     /gene="nagB"
                     /locus_tag="PLH17_02155"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOR20619.1"
                     /translation="MKVIIGSSQEISQLIADEFIKQIRRKPTSVLGLATGTSPLEVYA
                     NLIKANKERVISFKKVTTYNLDEYIGLDGTHNQSYRYFMNASLFDHIDIDKNNTHVLK
                     GVGDYLDYAAHYDEEIARSGGIDLQILGLGSDGHIAFNEPDTPFDSLTHIADLTESTI
                     RDNSRLFKDISEVPTKAVTMGLKSIMNAKKIVLIATGKNKAKAVKDLLEGPISEKVPC
                     SILQKHSDCTIYVDADAYSKVNQK"
     gene            19584..21512
                     /locus_tag="PLH17_02160"
     CDS             19584..21512
                     /locus_tag="PLH17_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20620.1"
                     /translation="MKTRVRNQFLKILIGGLFAVFAISFTHFSDFNAHDVKGEEAELV
                     DCAYYFDESNGYTSIYEIVSGLNSSTLQSGTYKTWGTVTKYYLDSSQNKNFYMQSTDK
                     NGNTAGIMIYRSSISVKEGNVLTIEGFPTLYNNLPEFEDPSSIEVDYAANSSPIETLV
                     TSESFWQNGTDRNSSQFLSAQSLGTRKISIQNIALSSIYSTTATATIGSTNVVLYYAY
                     LSNSSEIYNALYSLSAQTVNLTGYLHCFFNGYEAKMQLLIRSTSDIFAEGVDYSLTID
                     ASNYTSIGTYSTGNYDEKSISGFIFEHYRAIAPINSNQEFIILLPYVNQSGDGSAPGA
                     LYNTTAINDIFNIAITYHTESTYGAKPVLSYGANPLCETQNELNLSTVATTVSLAMSD
                     VDFFKVETSETRLFIHSLEIDYASEGPAPAFSYLSSGANGVRINPITYSGELTNGASA
                     SVPISISQSGAHYTVQTTKTYTYYTYEHFAANPQLVAQAAYTEPEDVAAFYTIFGTYP
                     SNYVFRNNYSSAYSLFGDATRCVSVYSRTDGYATSVPYQADYSGYPLYYECDIAFDSS
                     YSSNNRGVGRVVCWDYGFDPAKGAVGYDDSPVAVYSDDHYATFQEYLNAGSFGARFNA
                     EMVRTGYVWGPAMTLLPY"
     gene            21561..22493
                     /locus_tag="PLH17_02165"
     CDS             21561..22493
                     /locus_tag="PLH17_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20621.1"
                     /translation="MDSLKTLIEKRQFQLVLDLTANTRGASDIPYRISAYIGLGKLDE
                     ALRLIKQYQGKFEDATFNIMKVHLEMLMTLNKYDIAYQELKYYQNLPYISQEVEEFLN
                     GAEGMIRTHERNFQRIKRKSKEEIIEILEKETDSLILLSALTEIRNYNINDFSTHLIK
                     LMARENINSFVGIYPLFLLVSGGYAQPLSLTKNGKLYTVVPKDLEPPFVNQNYEKVVA
                     LIEEVAKDPSLSEVAVSLFNELIIILYPENIFDESINLLSGALLAIAYDHFQIPRHDA
                     ALAEGLGIDEGDLKDLVRKFKQLLIENPPIKAVE"
     gene            22605..23879
                     /gene="tig"
                     /locus_tag="PLH17_02170"
     CDS             22605..23879
                     /gene="tig"
                     /locus_tag="PLH17_02170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOR20622.1"
                     /translation="MEKKITKLEQSKIEVLVSVDKEMWAKAQKDAFNKLAANVEIKGF
                     RKGKAPADMVKAKVDQGQVFDRAINSLLQDTYRQVIAEENLRPFARPVVDVTKLSDLE
                     LELKFTLVLAPEVTLGEYKGLHAEKKEVVVTDHEIEHAIEHLVHDNASLVVKEDTAVN
                     GDTVVMDFEGFVDGKPFEGGKAENYSLELGSGQFIPGFEDALVGVKAGEEKDVNVTFP
                     TEYTPELAGKAATFKVKVHEVKVKKIPELNEELIKDLNLPNVKTLDELKSYQKAKLES
                     QKANDVKRDYYEALVKQIRDNAKIDLAKEIIDGEVEAMEENMLKQIEQQGLTMEKYLQ
                     ITGQTHEQLHEQMAKEAADNVRSVLVLEKIAEIEKITVEDDEIEFELAKIADQYKMPL
                     EEVKKVLKDSMDRFRADVRSRRIQEFIVNNND"
     gene            23911..>26030
                     /gene="lon"
                     /locus_tag="PLH17_02175"
     CDS             23911..>26030
                     /gene="lon"
                     /locus_tag="PLH17_02175"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOR20623.1"
                     /translation="MDENLPLTLPLLVTRSLVVFPNTNQPIEAARPFSMKAIEESRDN
                     ASSLLFIVSQKDPNIDDPLPEDLYTVGTLCRIISFGDQKQFYRIRVTATKRVAIQSVR
                     IEDNHYVADGVVLEDLPSDANETAVIVRNILEQIEQMPSLSRSFPRQVVSSLSKGLSP
                     TELTDTLATYLNMPNEQKQALLEELDINKRLMLILDVINQAKAVIEIDKKIQNSIQKS
                     TEKNQREYILREKMKAIKEELGEVTPGEDSEEDILKKLDANPFPEYVKTKVKAELKRY
                     EMMPQASLEASLIKSYIDLIMNLPWYQKTEDNDVLEDAERILNEDHYGLDKVKKRIIE
                     YLAVKKMTGNLKAPILCFYGPPGVGKTSLGKSIARALGRKFFKASLGGISDEAEIRGH
                     RRTYVGSMPGRIISGMRKTGVINPVFLLDEVDKLSTSYKGDPASALLEVLDPEQNFAF
                     NDNYVEEPYDLSNVLFIATANYLENIPAPLRDRLELIEVNSYTELEKIQIAKHFLLKK
                     QMKANGIENKNIKFSDEAFQYIIQYYTREAGVRELERKIAAVIRKIVVEIVKNPNLKV
                     LEVTPADVRKHLGVEIFENTKKEKENQIGVVTGLAYTEYGGDILPIEVNYFPGKGNLV
                     LTGKLGDVMKESASIALDYVRSNAIKYGIDSDIFAKNDIHVHVPEGAVPKDGPSAGIA
                     ITTAIVSCLTRQPVDANVAMTGEVT"
BASE COUNT         7579 a         5934 c         6031 g         6486 t
ORIGIN      
        1 atttgctatt aacgccgttt cgtgagtatt atataataat aaagcgttga cgaagacacg
       61 cctttgacaa tttagcgaga taacgagagt gcaaggttat catcagtcaa acggtatccc
      121 ttctgagcga gacctgaaat tatgtaggga atccgtacat ccgacgttac cggatatcaa
      181 gggttatgca aagattgcat aaaactaagg tggtaccgcg cgattagcgt ccttagatga
      241 ggacgctttt attttgaaaa gaggaagttt atggaactca agaaaacact attaatgcca
      301 aagaccagtt ttgagatgag aggaaatctc aatctcaagg aaccgctctt tttagcgcgg
      361 tggcaagagc aagacttata tcaggcaatg cttaaatcac gcgtcggggc aaaagaatac
      421 gctctccacg acgggccgcc ttacgcgaat ggcaacattc actgtgggca tatgctcaat
      481 cgtttactta aggatttcat tgtccgctat aagcatatgt ccgggtttta tacaccgttt
      541 gtctttggct gggacaccca cggtcttccc atcgaaaatc aggtgaccaa acagggcgtc
      601 aaccgcaagg cgacgccgat cgtcgaattc cgcaagaaat gccgcgaata tgccttgaag
      661 caagtcgaga ttcaaaagga acaaatcaag cgtttaggcg tagtcggcga ttttgaaaac
      721 ccatacgtca cccttgataa acaatatgaa gcccggcaga ttgaagtgtt cgccgagatg
      781 gcattgaagg gtttaatttt taaaggatta aaacccgtct actggtcgcc ctcgagcgaa
      841 tccgctttag cggaagccga gattgagtac tatgatgtca cctcaccatc aatttatgtt
      901 agttttgatg tcgtcgacgg taaaggcatt ttaaatcgag gcgataagct cgtcatctgg
      961 acgacgacgc cgtggacttt gccggcgaac ctcgccatct ccgtccaccc gcgttttgaa
     1021 tatggtcttt atcagacaaa caaaggccga ctgatcctcc ttaaagagtt caaaaaccgc
     1081 ctccaaaaag aattagacct tgtcgaaatg gatttgctta aaacattcaa cggtcagcaa
     1141 ttagagggcg ttttggccaa acaccccttt tatgagcgtt catcgctcgt gcttaacggc
     1201 gaacacgtca ccaacgaggc tggaaccggc ttagtccata ccgctcccgg gcacggcgtc
     1261 gacgactata atgtctgcca gaaatataat attctgcctt attgcccggt cgatgaccgc
     1321 ggcttcatga caagcgaagc cggcgagagg ctggctggtc tcttctatga agaagcaaat
     1381 gatgttgtcc tggcgatgct gagagaaaat aacgctcttt tatgcgaaga caaaagttta
     1441 cacagttatc cccatgattg gcggacgaag aaaccgctca tctttcgcgc cacgccgcag
     1501 tggttctgct caatcgaacc gattcgcgag agcctgatga atgaagttaa aaaaatccat
     1561 tgggtgccct cctggggcga aacgcgcatg ctcaacatga ttaaagaccg caccgattgg
     1621 tgcatctcaa ggcagagagc ctggggcgtt ccacttccca tcatctataa cgaagacatg
     1681 accccgatta ttgaaagaga agtttttgat cacatcatcg ccctttttaa agagcacggc
     1741 agcgatatct ggtatgaaaa agaagcggtg gatttgctgc cgaaaggata tcgcaatgcc
     1801 cgctcgccaa atggtctttt ttctaaagaa aaagatatca tggacgtctg gtttgacagc
     1861 ggctcctctt tcaatggtgt gatgcgcgag cgcggactaa agttcccggc cgatttatac
     1921 atcgaaggca acgaccaata tcgcggctgg tttaactctt cactcaccat ttcgaccgcc
     1981 gtcttcggca cttcgccgtt taaagattgc gtgacgcacg gattcgtggt cgacgaaaat
     2041 tgggataaga tgtcgaaatc gaaaggcaac ggcatcgacc cgagcaaagt cgccaatcag
     2101 ttcggcgccg atattttaag attatgggcg gcgacgatcg actacaaaca agatgtgcgc
     2161 atctccgagg gcatcatcac gcaagtcgcg gaagtatacc gcaaggtgcg caacaccttt
     2221 aaatttttac tcggcaatct cgcaaacggc gagcattcaa acttttcgcc aagcagcgat
     2281 aaagtcgagg tgttcgaggc cgtcgatcgc tttattctcg ccaagctcga aagcgtcaaa
     2341 aacgaggcga taggcgcttt tgacaaatac gatttcggca acggcatcaa ccgcatcgtc
     2401 accttcatgt cgagcgattt gtcgtcattt tatctagata taaccaagga tgttttatat
     2461 tgcaatagcc agaaatcgcc tagacgcctt caggttcaaa atgtcatcta tcaggtgacc
     2521 gatactttaa tgcgtttgct cacacctttt ctaccgttta cgatggacga aatttatcat
     2581 agcctgtcat atgcaaacaa ggataatgtc cagctgctcg actatccgag gatctcccat
     2641 cagttcgatg aaagtataat caaagattac caagccttga tggagcttcg tgatatcgtc
     2701 ttaaaaaaac tggaagaggc ccgatcctca gggctgatcg gttcacctca ggaggctcat
     2761 gtctcgctag aagaaaataa tgcattgctc aagaagctta aactagatga aaacgaagcc
     2821 gaactggcgc gtctttttgt cgtctcaaaa gttgatctta aggcaaaaga tggagttctc
     2881 atcacccggg cggaaggcga aaaatgcccg cgctgctgga attatgtcga acagttgcat
     2941 gaacacgaag aacacgccgt ctgcccgcgc tgcctcggcg tattaaagga gaatcacgat
     3001 ggccggtaaa atcgctaatt attttaaatc caatagcatc aaagagatcg cccgcgattt
     3061 cttcttatcg tttgtctggc tcatcgtctt gcttttcgtg attgacctcg tctcaaaatg
     3121 ggtcgtcgta aataatatga atgtctacac tgaaatcacc gtgatcgaaa acttctttta
     3181 ccttcgcctt tcctttaatc agggcgcggc ctttggcctc ggtaacaccg gcgatatcga
     3241 gtggcgcatc ttcttcatcg ccgtctcggc cgtcatcggc acggcgatgc tcgtctacta
     3301 cataaagaaa tacagaactc tcagtatcgt gatgaaaatc gccgtggcaa tgatgattgc
     3361 cggcgcctac ggaaacttga ttgaccgcgc tttatactgg ccttcaactg tcggttttag
     3421 cggcgtggtc gacttccttt ccttcgaatt ctgggggcgt cgctttgcga cttttaatgt
     3481 cgcggatatg tcgcttgtcg tcggcgtcat catcttgctt tttgccatcg ttatcgaaga
     3541 catcagaatc gcaaagaaca aagaaggcaa agaagattta tcaaagccct ctgccgagta
     3601 tctcgcggat aaggaaaaga agcatggcgg ggaagacgaa aaattataaa gtcgatcgcg
     3661 aagccggtca gagaatcgac aaagccctgg tcgagatttt aatcgatgtc tcgaggagcc
     3721 atattcaaaa actgattgat gagggttatg tcttcgttaa taacgaagca gtcaaaacca
     3781 gctaccgcct gcaaaagggc gatgtcatca acatcgtgac aagagaagat cagccttcgg
     3841 atttaaagcc cgaatatatt ccccttgata tcgtctatga agatagcgac atcatcatca
     3901 tcaacaagcc gcgcggcctc gtcgtccatc ccggcgccgg caacaaagat ttgaccttgg
     3961 ccaacgcctt gatgttttac acgaagaatc tgagctcgat caacggcgag tttcgtcccg
     4021 gcatcgtcca tcgcatcgat aaggatacat ccggcctctt ggcggtcgcc aaaaacgact
     4081 atgcccataa cgaactggcc gcccagctta aaaatcacac gatgcagcga atatacgtcg
     4141 ccctcgtcca cggcgtaatc catgaagaag aaggaaaaat catcgctcct ttggcccgcg
     4201 ataagaaaaa ccgcctgaag atggcggtcg atcttcaaga gggcaaagaa gccgtcacgc
     4261 actttaaagt tttaaaacgg tatcaaacct cgacgttagt ggaatgccgc ctggaaacgg
     4321 gccgcacgca tcaaattcgc gtccattttg aatatatcag gcatccgttg gtgggcgacc
     4381 ccctctacgg aaagaaaaaa gacgctctat atcaaggcgg gcaattatta cacgcgaagg
     4441 ctttgacact catccatccg cggaccaaag aagtgaagac atttgcctgt gcgttaccgg
     4501 catattttga agaaatactg aaaaatttat aattgagtat gtggcaataa taaaaaatgg
     4561 gcaaaaggcc catttttatg ttggattgct gataagatta tctttcttgt aggccaaata
     4621 cgagacccat gtaaccagcc gtataatcag tcggcgaggc gccgatgctg acatacatat
     4681 taagatatgg gccggtgaaa tagttggttt gactatagac atatggggta attggattat
     4741 tatcaagacc aacgagcttt tcaccatcga ataaggcttg ctgaccatcg cttgattcaa
     4801 agcgtggcat agccggcgga tacggggccg agttggtcat gaaagtgccg ttgaaatcaa
     4861 gggctgcgcc atcatcgatt gtcatgcttc cgctgatctt cgtcgatgag ggttccgaag
     4921 cgccgccgtc tttaacgact ccgatcatgt taaagtgagg aatgtcggtg ccgttgatgt
     4981 tgattaattg tgtaatcgag ttcgttccgt agtttggcaa tacttgctcg ccaaagccga
     5041 gaatgctcgg tagagcggcg tcggcatgaa cgagtctaaa ccagttttcg tttccggtga
     5101 cgattgattc ggcgactgta ttagtaaagg tggtgtgcga aggatttcca cccgttccgt
     5161 cctcttcggg actgacatgg tcggcgatga tgattggtcc gcccgcgccg ataaactcgg
     5221 aatcgataat ttgcagatta tcagtacccc agttatagag catgctgcta aacgaatcat
     5281 atcctcgcgt tttttcgatg atggcattcg ggcccgaaag ttcggtcaaa atgaaagtaa
     5341 agcattgcgt gccgatgaga ttgtagatat gggaatcaac actcaacaat ttggtgaaga
     5401 tggcgcctcc agacttagct ccggcttcgg tgcggttggc attgccaata accctaaggt
     5461 tgcgcatagt gtaatcacct aaatcttcgc cggtctgagc gtatcccgct ttgataagcg
     5521 ttgcgtgaga gataacaagt ccgacgtcgg cgtcgatacg acctgactcg cgagcgacga
     5581 ccggtagcgt cgagtaatca atgctgaagt aattgccatt aaagacgaac gcttcgccag
     5641 gcgcgacatc atggtgataa atatcgacat tgtcacgtag tgaaccaagg gcgcgttcat
     5701 agtcgccatc gccagaatta atgtccgaat cgccttccat atagaaaaag ctatccggaa
     5761 gtttgctgcg ttcgatttcg atatcgccgt gcagaaagag accgttaatt gccacaggcg
     5821 tgatgccttt ggaagtccga tatgcatccc agtgagtgcc gacgttgtta aaatgcgata
     5881 aatcatcttg tttatagacg ttatatccat cggcaacatt aaattcaaaa gaagtggaaa
     5941 atttcggatt gacggcatat tcatcgccaa aaggacgaac ggtcaatcta tatgtgttgc
     6001 cgatcgcttc ttcgctgaag tcatatgtcg atgcaaaatc atcgatggtc acgacgccat
     6061 ccagttcggc gccagtcaag atgacccatt cagttccgcg cttttcctca acgaccgaga
     6121 cgctatgata ggcggtgacc tccatctcga ttccatcgcc atcataggcg tagatgttgg
     6181 gaaagaattt aaacggatta tcgtcaccga cgaagtagcc ctgttcgcga tccataaact
     6241 cattgcgttt gttggctacg tccttggctt cgatgttggc gaggtaagtg gtgacaaaat
     6301 caggagcgga gatattgtca atttcatcgt attcgacaac cgtataggtc cacacgacgc
     6361 tttcggtcaa atacgtgata gttatttcgg attcgccgag cgttgatgta tcaagcgttt
     6421 cgggatcgaa tgataaatcg cttccttcaa tggtgactgt ctttttttca aaagtggcgg
     6481 tcacgctgat ttcgtcgagt tcgacaatgg tatattgaac gtattcttgt tttagtccag
     6541 aagcgacttc gatgtcgagc aaatcgccga gctcttcaat aggcggctca cagccggcga
     6601 gcgacatggc gactaacggc aaaagcaata gactcttttt catggttgta tcctttcttg
     6661 atatttcccc gtgaatattt caagcatgcc gggcatgccg gaaatcattg tatcacttag
     6721 ttctaaaaag attaagcatc aggaattttc gcccggagtc gcaccttggc ggacttattt
     6781 gaattgtcgt tggcgcgaat cgtaaacgtc tcggttccct catcgatgaa aaagacgcgc
     6841 ccggtcgcgc tttcgacgcg agcaataaag ctattcggat cagtcgaaaa gctgacgctt
     6901 tggttcgtgg cttcaaaagg caggacttcg tagatgatgt cgaccgtcag acccggagca
     6961 taggcaagcg tcaaaggcga tccgggattg tccggattat cgatgcgata gacatttact
     7021 gactcgtcaa aataagtgtc atagtagtca tcgccgacac gaacgccgct gcagattatc
     7081 tccgacacat aaattgtcac gttatacacc tcggcgcgct gaaagaggag gccgatgacg
     7141 atgatggcca cgagggagag ggcgccgatt atcaagatga ctgatttttt catatttagc
     7201 tgatccgatc gtaataataa gctttgatct tttcaatata catatagacg gaggccccga
     7261 agaagacaaa accgatgagg agcattttca ggaatgcata attaaacgac tgatcgggca
     7321 taaattgttc ttcgacgaga aagccgaggg tgattaaggc gactaagaat gcggtgatga
     7381 aggcaaaaat cgtcgccttg gtctcattgg aattgctcac ttgctcgccg gcggtggcat
     7441 actgctcatt ttgcggattc atcaaatcga ggagggctga gaacaagatg tggccgactt
     7501 gaataaaaat caaggcgatg gaaagcagga tgatgttgct taaaacgtaa tgcatgtggg
     7561 cgttaaaaat ataaagagaa acgcacaatg agacgatgga aagcacgata ttcggcaaga
     7621 gtttggcgag cagaggaaag atgacgacga ccggctttgt ccgcttcata taggcggtgc
     7681 ggccttccct tgaatacatg gtggcgacca cgctgttgga agcgagcaaa ggcagcataa
     7741 taatcaagat attaaaagcc tggacggcga actgaccatt cgggtataaa tccatcgagg
     7801 cgaaaatccg attaatcagc aaaataaaca aaggaatcga aatatacgtg gcgatgaaat
     7861 tggaggtaaa ggtgccgctt cgaaaaatga ggcgcagctc tttaacgaca aatgataaga
     7921 aggccggcag acggcgattc agcaaggcct tgacccgaaa gcgcttctcg tattcaaagc
     7981 ttgcgctggt catcttgata aaaagaaagc gggctacgaa ataagccgcc ataaacaatc
     8041 cggccagcac gccgattaaa ctgataaaga cgagccagac atcgagattc aggtacgtgt
     8101 atcgcatcgc ggcgttatat ttgccgacga ccatcagcac gacataataa acgggtttga
     8161 aataatcttg gaaaaacatc gtgacatcgg agagcagaac tttgatcgga ccccagtagt
     8221 ttaagacgtt aatctccgac ggtatcaaaa gaataattct gacgagcaga tagatgaagc
     8281 ccgccaaaac ggcgataaaa aatagtattt ttagaactct gaaacggttg aaaaaccgca
     8341 tgataaaaaa gcccggtaga gagaggacga cacccaagac gacaggaatc atcgacacca
     8401 caaagaaaga gagaaaaacc cagatataga aaaaccaatt gacgggcgag atcaagccgt
     8461 aagccacaaa tatcggcaag gtaaaagtaa aatttttata tatctcataa agataaaaga
     8521 cgatgatttt agaaaggaag atgcgattcg cggaaatcgg ataggtgacg agcacctgat
     8581 tgtcttcggc catgtatagc gacttgatga gatcattcgt gctggcgaaa gtcgagatga
     8641 cgagaatgat tgttaagagc atcgtcataa tcgaagtcgg aataaagggc gaattatgaa
     8701 agatgcgcaa aaacgaggag acataaaata aggcataaga gaggccggcg acgatgataa
     8761 agcgcaaggc gaaaaagaca attttgcgta tcaattgcgc cttgtcttta ataaacccga
     8821 gatcgagttt atccttcaac tgcatcatta tcagcgtctt gatgtcgttc ataaattgcc
     8881 taggcgattt ccttggtggc cttttgttca ggcgtttctt cgtgaatcgc catctttaag
     8941 tagtattctt caagcgttcc ttgctcgtga acttcatcga tcgtcaaagg agcgaaaaga
     9001 tggccttttt tgatgacggc gattttctga cagagatttt cgacgacatc gatgatgtgg
     9061 ctcgaaaaca tgacgatatt gcctttggcg gcgtgctgtc tcatgcattc tttaacttga
     9121 taaatgctgt cgggatcaag gcccgtgagc ggttcgtcga ggagccataa tttcggctcg
     9181 tggacgaggg cggcgataat cgtcaccttt tgcttcatgc cgtgcgaaaa agttttaatc
     9241 tgattgtcaa aagcatcttc aagatgaaag aggctaacat agtgaggaat gcgcatttgc
     9301 cgctcctcat aggtgacgcc ataaatatcg gcgatgtagt tgacgtattc gcgggcggtt
     9361 aatttttcat atagagcata gtgatcgggg acatagccga ttaaacgctt ggccatgacc
     9421 ggttgctttt cgacatcgta tccgcagatg gaaatagacc cttcggtaat cgtctgaatg
     9481 ccgacgatcg atttaatggt ggtcgacttg ccggcgccgt tggggccgag gaagccgaaa
     9541 atctcgccgc cactaacgac taaacaggcg tcttttacgg caaagtcttt gttcttgccg
     9601 tagcgttttg aaaagttttt cagctccaat tttactcctg tattttcttc catgggttca
     9661 tcgatccccc ttccgttcaa ggctgccaat aaggcatatt tatcgagttt aatttttttc
     9721 tggcgaaagc gcaaatcacc gagcgaatcg gcgatgctga aaccgaggct gtaaacaaac
     9781 cacatcgcaa aggaaacacc gacatagacg attaagaaaa gagcttggta gacagggtga
     9841 aattcaaaga cgagatcgcc gatgttaaag ctgatggcga tgttgaagat gttctcggcc
     9901 cacctcccta atttatcaac gataaaatcg ctgttgataa agaagaaatc ggtcggcgag
     9961 taattgctga accagacatt taaaaccatg accagtaaaa agtagccgga aaaagcgatc
    10021 agcgaataat acatctgctt cattttcggg cgcgggaaaa gcttcagggc gacgagtaat
    10081 aaaggcatga aaaaagctag agaatggtta taccaaaact ggatgatgac ccagctcgtc
    10141 aagttcgtcg tttccgcata gttgggcatc agcatagcca tcagagcccc gaagacattg
    10201 atgaagtagg tgaagtagaa aagcttgtcg agtttgaaga tgagcactaa aggaatgata
    10261 tacatcgccg tgttgcacaa gtgaatcggc cacgtccacg gctccaagaa gttctgatag
    10321 taaaaaacac gggagaaagt aatcatcgtc acgacgctta gatagaccat cgccaaacgg
    10381 atgagttcgg gatctttctt ttttaaagaa aaatatagga ccagcggtat gataaccgtc
    10441 gcgtagataa agaggcggtg aacaaaggag atatcgatca cttcgtagcg ggcattggcg
    10501 tttccaaaca tgagctgcgg gccataaatg ggaagcgatg cgacaagcac gggaacaatg
    10561 gcgatcaaga gagcaagaat ctctttttta tccaatttta aagtgtgatc atcgcgccag
    10621 accaaaaata agccataggc gacaagagcg atttctaggg cgaacatcac actttgaaaa
    10681 gataaaacca aggttccgcc ggtgttaccg atcacttgaa gaaaaacact gtaataagag
    10741 aagccgagat aaagaacaaa tcccggggtc agaacaaatt taatcaagtt tgtcagaatc
    10801 cgcgctttaa aaaacggata gaggatcagg aagacgacga gcgtgagttc gagccaaatg
    10861 ccgaagtagg caaataaatt aacaactgga gaaaaaggtc cggcgccaag acttacgata
    10921 ttgaggattt gatcatcata ggatatgtag cgggtaaaaa agacggcgat gagcacaaaa
    10981 gcaaagattt tcttgccgag cgagaaattc ttattgcgag ccaaaaaaga agtggtcaag
    11041 gcacctgccg aggcagttat taaggcgatg ataagcgaca agtaatgtgt caattttatt
    11101 tcctgttagc cgcgtcccgc aacccgcgca aggcggggcg aactcggtag cattatagca
    11161 ttgaaggtct tatttgttaa tcatcttaat gattgctcat aaaccgtggc aaacgcgcgc
    11221 ccactcgctg ctgagtatat cgttataaat gtcttctatc gaaccatatg tctggctgac
    11281 ggtgccgtta gttgaggtgc cgtagtaaga catttttagc ggcgaaccct ccatgtaaac
    11341 tttgtttaaa ccatcggtga caaaagaagt cgtgccatta acgaagttta aatatatttt
    11401 gggagccact ctcttttgcc ctttagttaa aaagcgcgat aaggcttgaa gatgggggtg
    11461 tccggcgcag gcaccggtct ttccgccata agaggtgtct ggtgaggggg cgccggctga
    11521 aatggtaacc agtttaggtt cgagggtgtt gagcaagata tcattatttg cttttgaaga
    11581 ggagtggtga gcggctttca tcatcgtgac ttcatgaaaa atttcgttat tatctccatt
    11641 aatggcgacc agattagtct cgccaagtcc accggaaccg ccattataac catccgcatt
    11701 atcttgtaaa tcgccggaga agaagaaagt gaaatcgcga taggcgagaa ttccggccag
    11761 cgaagtgaca ttgatgtcat cgacgacggg gccgtcgtta tagttctccg tatagtaacc
    11821 ggtatcgagc caatcaataa aaagatcagg cgtgatgtag gttcttttct gaccgccatc
    11881 aatttcattg acattatcat agatgcggtg gtaggcactt cctttatcag catagtaatc
    11941 gcgaatgtct tttcgataat tttcgccgcc gctactgtaa tatccgtagt cgacaatcat
    12001 gccgactgaa tcgattgcgt ctttcagggc ttgatagccg ccgtaatggt cgccgtgagg
    12061 atgggtgaga atgagcagat caagatggcc gtcatcgata taagtttcat aagtattttt
    12121 taactcggaa taataactcg tctggtcagt atatttataa ttggatgggt gaccaaaatc
    12181 catggcgatg gtcgcctctc cggctctaaa taaatatgcg tcgccatatt gagatggttg
    12241 ttcgaaagtg agaatttcaa gatattctgc cgatgagttg gatagcgggt gagcggcgct
    12301 tccaagcggc gccaattcat caaaagacgc aacttctttt tcggttttat ctagtgcgtc
    12361 gatggcgatg ccggtatcgg ccatcgtgct gattctttct tcataagaac cgccgatgat
    12421 atcggaaccg acactaatat tggacgatgt gtcagaaagt gtgctatcac gcggttcgca
    12481 ggccgtcagc aagagagcgt ccaagagcac gaaaggaaat actcttttca tttttcggtc
    12541 tcgtttttgg tgacgcgctg gtaattttta aaatcggtct tggcgatttt gcgtaattcc
    12601 tcaatgccgt gcttttttat aaaagcaaga gcgaacgaat cgacttttga ggaagctccg
    12661 aaagggatga tggcagcata ttttttgctg agtgcttcca tctttttcaa aaaggaataa
    12721 cgagcgatga cactggcgag agccacggag gggtagcgcg attcgccctt cgtcttaaaa
    12781 gtgatgtcac gaacgatttc ttcatcgcgg gccgcatagg aaaaatatcg ggtttcggat
    12841 acaaatttat cgacatagac gttcttgacg tcgtgtttct tgattaaatt aaggagaacg
    12901 cgattatgca agcgggcttt aatctcgttg atgttgatgc ctcgcttgat gagctcatta
    12961 tatttttcgt tcgtgagact cacttgcgaa taagaaaatt ttttcagtaa cagcggggtg
    13021 atatttttta tgaagtcatc gcttattttc ttggaatcag cgacgccgat cctcttgaga
    13081 taagcaacat cctgtttttt aacataggca gcacagacgc agatagggcc gagaaagtcg
    13141 ccggtgccga cttcgtcgct gccaatctgg tcatcgattt caaaccatcc gagcgtctcg
    13201 tcgtcttctt ttatatcctc ggaaatggtg atagttttat cccagcgcct cgcctcataa
    13261 atgaagcctt cgcccgaaaa agtaatttta gtatccttgc ctttggagtt ttgataggcc
    13321 gtgatgacta ctttcccttt tttggcgcga aacaaaacat attgaccagc atcaagaatc
    13381 tggtcatttc ggtaatattc ccgtattttt tcaagaagtt cctgattagc tttcagcgtg
    13441 acgatgttgt tgcccataaa tgccgatatt attctatcat aaaagctgct ctatcaataa
    13501 cgaccatatt ttatggtaag atattgtcat gccaacgatt tacgaagtcc ttgaattcgg
    13561 tcaaattcgc aaagaaatag ctgcttttgc acagagcgat atcggtcgcg aaacatcttc
    13621 aaatttagtg atgctttcga aaggcgaact caaaaatgaa ctcgccattc tcgatgagat
    13681 gatggtttta aatcttcgct atggccgcct gccgatgatg agttcaacca acctgagcca
    13741 aaaagtcgaa tatgccttta aaggcgggac tttgacggcc gaagacttag aagcgattgc
    13801 ccatgatgtt atcatcaccg gcaaaatcgt tttgtcaatt cgcaagcgcg aagaaaaatt
    13861 tccgctcctc aacgacaaga ttcgccagtt taaaagcctc gagcaactcg aagccgccat
    13921 tcaccgcgtc gtcggacccg accttggcat attgaatagc gcctccaaaa agctggcttc
    13981 gattcgcaaa tcaatcaccg aaacggaacg aaaaatgaca attcgttttc agtcggtgat
    14041 cgagtctttg cgagactata tgacggaggc ggttgccacg attcgcaacg gccattttgt
    14101 cctgccgatt aaaaccgctc acaaaagcaa agtcagcggc gttattcacg acatctcgga
    14161 caccggcatg acgaccttca tcgaacccga ctgggcggtc gaatccaaca atgcggttta
    14221 tgtcctgaaa atcgaagagc gagaagagat tcagcgcctg ttaaaagagc tgagcatcct
    14281 ggtcgccaca cacggccatg aagtcctcgc caacaaccat acaatcggct atcttgattt
    14341 cgttcaagct aaagctctct atgccatttc tctcgacggc aaaattgccg agttgagcga
    14401 ggaaagagcc atcgatataa agcaagcgcg ccatccgctc atcaatcggg aagttgtcgt
    14461 ggccaatgat ttttcttttg atgattcact tcgcatcagc gtcatcagcg gccctaacgc
    14521 cggcggcaag accgtcgccc tcaaaacgct cggtttgatg gttttgatga atcagtgcgg
    14581 gctggctatt ccgacaataa aacccgcaaa aatggcgtat tttaatcata tttatgcaga
    14641 tattggcgat aatcagtcgc tggccagcaa tctatcgact ttctccggtc atatcaaaaa
    14701 ccttgccgaa atcgcggcgc ttgtcgattc cgacgacttg gttttactcg acgaaatcgg
    14761 caccggcaca tcgcccctag aaggtgaagc cctcgccatt tcatatcttg aatttcttcg
    14821 cgatatcggc acctttgtct tttgctcctc acactatgac ggactaaaag attatgcctt
    14881 aaataaagat ggcgtaacca acgcttccgt cctttttgat gagcagaaaa tgacgccgac
    14941 atacgtcctg aaacaaggcg ttccgggccg cagttacggg cttgagatgg ccgctcgtta
    15001 cgacctcgcc aaaccgatta ttgacgaggc tcacaacatc ctgaaatcgc atggtcatga
    15061 acagagtatc agcgctttgg gtaacctcca aaaattaatc tccgaaaacg agatcaaagc
    15121 ccacttgctt gaaaacgaag taaagcgtat gcagaacttc gaaagaaccc ttcaggaacg
    15181 ccagcaaaaa ctggatcagc aaaggcaaaa cctgctcgaa gatgtcagcg atgctaaaca
    15241 ggaaatactt gctcgagcgg aaaaagagat agatgagatc tttgccctgc tcaggaatcc
    15301 cgatattaag atgcatgaag taatcgaagc caagggtaaa atcagaaagc taaaagaaga
    15361 tgaaaccgat gttgtcttat ccgaaaatgg cgacatcgaa gtcggcagct atgtcgctat
    15421 cactgacttg ggcatcaccg ggaaaattga aaaaatcagc ggcaatcgcc tttttatcgt
    15481 gtccccggat gggttaaaaa tcacttcaac cagagaaaaa gttcaccttg tcggcgctcc
    15541 ggctccagaa aaggccgaaa agacattcgt cgccccgcaa attgtcagcg atgtcaaatt
    15601 agaacttaac gttatcggtt taagagtcga tgaagccctt gaagaagtcg cccgctatct
    15661 cgatggcgtc cgcctcaagc atttctctca ggtgcggatt attcacggtt ttggaaccgg
    15721 ggctctaaaa cgagcgattc acgattattt gtccaagtgc gattttataa aaagttatcg
    15781 acccggcgac cagcacgaag gggccggcgg ggcgacggtg gtgatcatga aatgaccgaa
    15841 gccgtaaaac aggccctgtt attagtgccg accaaacccg gcgtctacct catgaaagat
    15901 aaagatgagg aaatcatcta cgtcggaaaa gcaaaaaatc taagcaagcg ggtgacgcag
    15961 tattttatgc ggccgcaatc cggcaaagtc gcggccatgg tttttaatgt cgaccatttc
    16021 gaaacgatca tcaccaagag cgaaaaagaa gcctttatcc tggagatgaa tctaattaaa
    16081 aagcattatc cacgctacaa tattttactt aaggacagca agcactatcc ctacattgcc
    16141 ctcaagcgga aaaatagccc ggttttaaaa atcgcccgcg atgataaaga caaaaattat
    16201 ttctattttg gtccgtatcc gacatcgatg cacgcttacg aagtgatcag cttgctcaac
    16261 aagatttttc ctcttcaaaa atgcaaagtt ctgccgccgg tgccgtgcct ttattaccat
    16321 cttggccaat gtctggcccc atgcatcaat aagatagatg aagagacaca tgtcgccatc
    16381 tataatcaga ttaaaagctt tcttggcggc aacaacgaag atgtcaaaag tcaaattcgc
    16441 cgcaagatgg aagaagccag cgataatcag gaatatgaaa aagccggcga ttatcgcaag
    16501 actctcctcg ccatcgatca catcaacgaa cgccagagca tggaagttaa agaccatgtc
    16561 gaccgcgata tctttgccta tgtgactaga gacggttatt tagctttggc tcttttaact
    16621 ttccgccacg gcatcatgct cggcaaagaa gtctttgtcg tcgagcaatt tggcgaggat
    16681 atcgatctcg tcaccgatct gattttgcag tattatcaaa cgcattctct gccgaacgag
    16741 atcgtcgcta atatctatgg tttaaaagcg agaattgaag atatatacga cataaatgtc
    16801 gtctacaaaa gtcgtggcgc gctttacgac ctcgtcgaca tggctacgat caacgcccgc
    16861 cagggtcttg atgagcactt tatgtccgcg cggttaaccg atgataactt aaaaattctg
    16921 accgaacttg gcaatctgct aaacatcaaa actccgacgc acatcgaact ttttgacaat
    16981 tcgcatcttc agggttcttc tcccgttggg gccatggtcg ctttcatcaa tggcgagccc
    17041 acgaaaaaga tgtatcgaaa atttcatatt gaaggcgatg aaaagcgcga tgattttgcc
    17101 agtatgaagg agattatcta tcggcgttac aaacgcatta aagatgaaga tttaaagatg
    17161 ccgaacctca ttctcgtcga tggcggactg ccgcaattaa gcgccgctta cgaggcactt
    17221 gagcgactca aattatcaat tcccgccttt ggattataca aaaatgatcg gcatcagact
    17281 gagggattaa tcgacaaagc aggtctaaaa tacgacttac aaaataacaa gccgctcttc
    17341 ttcctattga tgagaatgca agatgaagtg catcgctttg cgattacctt ccatcacaaa
    17401 ctgcgcagca aagcttacaa ggcgagcgtt ctcgacggaa tcgaaggtct cggcaagcgg
    17461 cggattgaaa ttttgtaccg caactatgcc tcgatcaacg aacttaaaaa agccaccttg
    17521 gaagagcttc aacaactgct gcccaaaaat gttgcttcaa gggtcaagga aaggctggaa
    17581 gaataacctt ttttgtgttg atatgtcatt gctaaaatac tatattagta tagcgatatg
    17641 tcctcttagc tcagctggat agagcaacgg ccttctaagc cgtaggtcag gcgttcgaat
    17701 cgcctagagg atgccattgt aaagacgagc tttgattgca ggttaatcaa ggctttttat
    17761 ttctcaaaac acaaaaatgt aaggaaaacc ttactttttt tgttatgata aattggtcga
    17821 cttccccttt tcacctatcg ataattatgg caggagacaa atacatatga gctttgaaaa
    17881 aaacctgaat ctaatcagaa aaagaaacgg catgacgcag gaggatttag ccctgacaat
    17941 cggcgtctca cggcagacaa tctacgcatg ggaggcaggt ttaaattctc caaatatctc
    18001 catgctcaag aaggtcgctt ccgctttgga ggtcagcatc gatgaactga ttaacggcta
    18061 tgatgtcgat cgacttccta atttattagg agaaatagtt tttagcaacg aatccgagta
    18121 ttacgaggat gtaatttatg aggaaattcc caattggtat gtttccttaa aggtcggtga
    18181 agatgtctgc tttggcttgt atgataacgg cgtaaaggat tactcatacc acgtcactgt
    18241 gttaggagaa attattgttc ataatcaaaa gggttatgaa ataggtgtga aagaatacga
    18301 taaaaagtta gagcaaacgg acgccttttc gctgatagcc aaacgcgttg aaaaccggat
    18361 aattttcatc ggcagaattt attacaaaga cggaataaaa aacatcgaaa catttcgcga
    18421 caaaagtttt ttaaataagt ggggcaccga tggcaagttt gaaggtccgc cgataatctt
    18481 tcaagaagcg aagaagtatg cgatgacata taacggcaaa accgagagcg tcattcgcat
    18541 cgcttacttt gatgcaaaca actcttacat cgaggtcttt ttaaatctta agggcgagtc
    18601 gttgttttgg aggcgatacg aactcggccg caaatcagga actattctta aaataaacga
    18661 taaggagtat ggtttattct atgaagtcgc gacttcgcgt ttaataagat aggtttacac
    18721 taaaaatcat tttacacatg tccgatgatg gtttatcata tttggcggta ttttactcgt
    18781 taggaaagaa gatttaaaag tgaaagtaat catcggaagc agccaggaaa ttagccaact
    18841 catagcagac gaatttataa agcaaattcg ccgcaaaccg acctccgttc tcggtcttgc
    18901 caccggaact tcacctcttg aggtgtacgc caatttaatt aaagccaaca aagaaagagt
    18961 aatctcgttt aaaaaagtca cgacctacaa tcttgacgaa tatatcggat tagacggcac
    19021 ccacaaccaa tcgtatcgct atttcatgaa tgccagtcta tttgatcaca tcgatatcga
    19081 taagaataat acccatgttc ttaagggcgt cggcgactat cttgactacg ccgctcatta
    19141 cgatgaagaa atcgccagaa gcggcggtat cgacttgcag attctcggcc tgggaagtga
    19201 cggccacatc gcctttaatg aacctgatac cccatttgac agcctcaccc acatcgccga
    19261 cttgacggaa tcgaccatcc gcgacaattc ccgccttttt aaggatatca gcgaagtgcc
    19321 gacgaaagcg gtgacgatgg gcttaaaatc gattatgaat gctaaaaaaa tcgtgctgat
    19381 agcgaccggc aaaaacaaag cgaaagctgt taaagatttg cttgaaggtc ccatcagcga
    19441 aaaggtgcct tgttcaatac ttcaaaaaca ttcagactgc acaatttatg tcgacgccga
    19501 tgcgtatagc aaagtgaatc agaaataaaa aatgagccgc gattgaatcg cggtttcttt
    19561 tttgttagga tttaatggct attatgaaaa ctcgtgtaag aaatcaattt ctgaaaattt
    19621 taatcggcgg attatttgcg gtgttcgcca tctcctttac tcattttagt gattttaatg
    19681 cccatgatgt gaagggagaa gaagccgaac tcgtcgactg cgcttactat tttgatgaaa
    19741 gcaacggcta tacttcaatc tacgaaatcg tcagcggact aaactcctcg acccttcaaa
    19801 gcggaaccta caagacatgg ggcacggtga caaagtacta tctcgattcc tcacagaaca
    19861 aaaattttta catgcaaagc accgataaaa acggcaatac cgccggcatt atgatctatc
    19921 gaagttcaat ttcggtgaaa gaaggcaatg ttttgacgat tgaaggtttt ccgactttat
    19981 ataacaacct tcccgaattt gaagatccgt catccatcga agtcgattat gccgcgaact
    20041 cttcgccgat tgagacgctt gtcacaagcg aatctttttg gcagaacggc acagaccgca
    20101 attcgagcca gtttttatca gctcaatcat tgggaacgag aaagatttcg attcagaata
    20161 tcgcattatc atcaatttac tcaacaaccg caacggccac tatcggctca acaaacgttg
    20221 ttctttatta cgcctattta tctaactcgt cggaaatcta caatgcgctt tattcattat
    20281 cagcccagac cgtcaacctt accggctacc ttcactgttt ttttaacggc tacgaagcta
    20341 aaatgcagct tctaattcga agcacaagcg atatttttgc cgaaggagtc gattattccc
    20401 ttacaattga tgcaagcaat tatacaagca tcggcaccta ctccaccggt aattacgacg
    20461 aaaaatcaat ctcaggattt atctttgaac attatcgcgc gatagcgcct attaattcca
    20521 atcaggaatt tatcatctta ttgccatatg tcaatcaaag cggcgatggt tcggcacccg
    20581 gagctttata taacacgacg gccattaacg atatttttaa tatcgcaatc acctaccata
    20641 ccgagtccac ttacggagct aaacccgttt tgtcatacgg cgcgaatccg ctttgcgaaa
    20701 cgcaaaatga actgaatttg tcgacggtgg caacgaccgt ttcgctagcg atgagcgatg
    20761 tcgatttctt taaagtcgag acgagtgaaa cccgcctttt tattcacagc cttgaaatcg
    20821 actatgcaag cgaaggtccg gcccccgctt ttagctattt aagttcagga gcaaacggag
    20881 taaggattaa cccaatcaca tattcgggag agctgacaaa cggcgcaagc gcgagcgttc
    20941 cgattagcat ttctcaaagc ggtgcccact acacggtgca gacgacaaaa acctatactt
    21001 attatactta tgaacacttt gcagctaatc ctcagctagt ggcccaagcg gcctatactg
    21061 aacccgagga tgtcgcggcc ttctatacga tttttggcac ttacccatca aattatgtat
    21121 ttagaaacaa ttattcatcc gcgtattccc tttttggcga cgcgacgaga tgcgtttcag
    21181 tctactcgag gacagacggt tatgcgacat cggttcccta tcaagctgat tatagcggct
    21241 atccccttta ctatgagtgc gatatcgctt ttgattcgag ttactcatca aataaccgcg
    21301 gcgtcggtcg cgtcgtgtgc tgggattatg gctttgatcc ggcaaaaggg gccgtcggtt
    21361 atgacgattc gcctgtcgcc gtttatagcg atgaccatta cgcgacattt caagagtatt
    21421 tgaatgccgg atcctttggg gcgagattca atgccgagat ggtgcgaact ggctatgttt
    21481 gggggccggc gatgactctt ttgccgtatt agcatttcga aacgatagaa ataacctttg
    21541 gtttgatata atagcaatat atggattcgc taaaaacttt aatcgaaaaa aggcagttcc
    21601 agctcgtttt ggacttgacg gcaaatacgc ggggagcttc cgacatccct taccgcattt
    21661 ccgcctatat cggattaggc aaactcgatg aagcattgag gctcatcaaa caatatcaag
    21721 gcaaatttga agacgcgaca ttcaacatca tgaaagtgca tttggaaatg ctcatgacgt
    21781 taaataaata cgatatcgcg tatcaagagt tgaaatatta tcaaaacctc ccttatattt
    21841 cacaggaggt cgaagagttt ttaaatgggg ccgaaggcat gattcgcact catgaacgca
    21901 atttccaaag aatcaaaaga aagagcaaag aggagattat tgaaatttta gaaaaagaga
    21961 ccgacagcct catattattg tcggcattaa ctgagatacg caattacaat atcaatgatt
    22021 tcagcactca tttaatcaag ttaatggcaa gagaaaatat caactcattt gtcggcatat
    22081 atccgctgtt tttactcgtt tcagggggat atgcacagcc cctttctttg accaaaaacg
    22141 gcaagctcta cacggtcgtg cccaaggact tagaaccgcc ttttgtgaat cagaattatg
    22201 aaaaagtcgt cgccttaatc gaagaggtgg ctaaagatcc gagtttaagc gaggttgcgg
    22261 tctctctttt taatgaattg attatcatcc tctatcccga aaacatcttt gatgagtcta
    22321 ttaatctgct gtctggcgcc cttctcgcca tcgcctatga ccactttcaa atcccgcggc
    22381 atgatgctgc tctcgccgag ggcttaggca ttgatgaagg cgaccttaag gacctcgtcc
    22441 gcaagttcaa gcagctctta attgaaaacc cgccgattaa agctgttgaa tgagttaaac
    22501 agtcatcaaa aagccatctt tttttgtgtt tcgtttgaaa ctgatacgaa tgtattctat
    22561 actttatgcg attgcatttt tattaaggag aattatatat aattatggaa aagaaaatca
    22621 ccaaactcga acaatcaaaa atcgaagtcc tcgtcagcgt cgacaaggaa atgtgggcta
    22681 aagctcagaa agacgctttt aacaaattag ccgccaacgt cgaaatcaaa ggttttcgca
    22741 agggtaaagc tcccgccgac atggtcaaag ccaaagtcga ccaaggtcaa gtctttgacc
    22801 gcgcgattaa ttccctgctg caagatacat accgccaggt catcgccgaa gaaaatcttc
    22861 gcccctttgc ccgccccgtc gtcgacgtca ccaaattgag cgacctcgaa ctcgaattga
    22921 agttcacgct cgtcctcgcc ccggaagtta cgctcggaga atacaaagga ctgcacgccg
    22981 agaaaaaaga agtggtggtc accgatcatg aaatcgaaca cgccatcgag cacctcgttc
    23041 acgataatgc ctctttggtc gtcaaagaag acaccgccgt taacggcgat accgtcgtca
    23101 tggattttga aggatttgtc gatggcaaac cttttgaagg gggcaaagcc gaaaactatt
    23161 cgctcgaact cggttccgga caattcatcc ccggttttga agatgcctta gtgggcgtta
    23221 aagccggcga agaaaaagat gtcaacgtca cctttccgac cgagtacacg ccggaactgg
    23281 ccggtaaagc cgcgaccttt aaagtcaaag ttcacgaagt aaaagtcaag aaaattcccg
    23341 agctcaacga agaattgatt aaggatttaa atcttccgaa tgtcaagacg ctggatgaat
    23401 taaaaagcta tcagaaggct aaattggaaa gccagaaagc caatgatgtc aaacgcgatt
    23461 actacgaagc attagtcaaa caaatccgcg acaatgctaa gattgatctc gccaaagaaa
    23521 ttatcgatgg cgaagtcgaa gccatggaag aaaatatgct caaacaaatc gagcagcaag
    23581 gcctgaccat ggagaagtac ttgcaaatca ccggtcagac tcatgaacag ctccacgagc
    23641 agatggctaa agaagccgcg gataacgtcc gttctgtcct cgtccttgaa aagattgccg
    23701 aaattgaaaa aatcaccgtc gaagatgacg agatcgagtt cgagctcgcc aagattgccg
    23761 accagtacaa aatgccttta gaggaagtca aaaaagtcct caaagacagc atggaccgct
    23821 tcagagccga tgtccgctcg cgccgcattc aggaatttat tgttaacaat aacgattaac
    23881 tgtcaaataa taatatcagg aggtctttaa atggacgaaa acctacccct taccttgccg
    23941 cttctcgtga cgaggagctt agtcgtcttt cccaatacga accaaccgat tgaggcggct
    24001 cgcccttttt cgatgaaagc catcgaagaa agccgcgata atgcgagttc gctccttttt
    24061 atcgtgagtc aaaaagaccc gaatatagat gatccgctac ctgaagattt gtacacggtc
    24121 ggcactttgt gccgcatcat atcatttggt gaccagaagc agttctaccg cattcgcgtg
    24181 acggccacca agcgcgtcgc cattcagtcg gtccgcatcg aagacaatca ctacgtcgcc
    24241 gacggcgtcg tccttgaaga cttgccgagc gacgccaatg aaacggcggt catcgtccgc
    24301 aacatcctcg agcagattga gcagatgccc agtttatcgc ggagctttcc gcgccaggtc
    24361 gtcagctcgc tttccaaagg cctttcgccg accgaactga ccgatactct ggcgacgtat
    24421 ctgaacatgc ccaatgagca gaaacaagct ttgcttgaag aactcgatat caacaagcgg
    24481 ctgatgctca tcctcgatgt catcaatcag gccaaagccg tcatcgaaat cgacaagaaa
    24541 attcaaaaca gcattcagaa gtcgaccgaa aagaatcagc gcgaatacat tctccgcgag
    24601 aagatgaaag cgatcaaaga agagcttggc gaggttactc ccggcgaaga ctcggaagaa
    24661 gatattttaa aaaaactcga cgccaacccc tttccggaat acgttaaaac caaagtcaaa
    24721 gccgaattga agcgctatga gatgatgccg caagcctcgc ttgaagcctc gcttattaaa
    24781 agctacatcg atttgattat gaatctgccg tggtatcaaa agacggaaga taatgatgtc
    24841 ttggaagatg ccgagcgaat tctcaacgaa gaccactacg gcctcgataa agtcaaaaaa
    24901 agaattatcg aatacttggc cgtcaagaag atgacgggca atttaaaagc cccgattctc
    24961 tgcttttatg gtcctcccgg cgtcggtaaa acatcgttag gcaaatcgat tgcccgcgct
    25021 cttggtcgca aattctttaa agcttccctc ggcggcattt ccgatgaagc cgaaattcgc
    25081 ggccatcgcc gcacttatgt cggaagtatg ccgggccgca ttatcagcgg catgcgcaag
    25141 accggcgtca tcaaccccgt ctttctcctc gacgaagtcg ataagctgtc cacatcttat
    25201 aaaggcgatc cggccagcgc ccttctcgaa gtgctcgatc ccgagcaaaa cttcgccttt
    25261 aacgacaatt atgtcgagga accttatgat ctcagcaatg tcttgttcat cgcgaccgcc
    25321 aactacttgg aaaacattcc cgcaccgctt cgcgatcgct tggagctcat tgaagtcaac
    25381 tcgtataccg aacttgaaaa gattcagatc gccaagcatt tcctgcttaa aaagcagatg
    25441 aaagccaacg gcatcgaaaa caaaaacatt aaattcagcg acgaagcgtt tcaatacatc
    25501 attcagtact acacgcgcga agccggcgtg cgcgaactcg agcgtaaaat cgccgctgtc
    25561 attcgcaaaa tcgtcgtcga aatcgttaaa aatcctaatt taaaagtctt agaagtcacc
    25621 ccggccgatg tccgcaagca tctcggtgtc gaaatctttg aaaacaccaa gaaagaaaaa
    25681 gaaaaccaga tcggcgtcgt caccggttta gcttataccg aatacggcgg cgatatcttg
    25741 ccgatagaag tcaactactt tcccggaaag ggcaatctcg tcttaaccgg taaactcggc
    25801 gacgtcatga aagagagcgc gagcatcgcc ctcgactacg tccgcagcaa cgccatcaaa
    25861 tacggcatcg atagcgatat tttcgctaaa aatgacattc acgttcacgt gcccgaaggc
    25921 gcggttccaa aagatggacc aagcgccggc atagctatca cgacggcaat cgtctcgtgc
    25981 ttgacgagac agccggtcga cgccaacgtg gcgatgacgg gcgaagtcac
//
LOCUS       DAOSSL010000012        25877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2088_length_25877_cov_13.824413, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000012 DAOSSL010000000
VERSION     DAOSSL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 25877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25877
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_25877_cov_13.824413"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLH17_02180"
     CDS             complement(<1..782)
                     /locus_tag="PLH17_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOR20624.1"
                     /translation="MSKVVLPNYQDSILNVSNSLLKHYGAKTNYSGQKELDAILNKKY
                     RHIAVFLIDAMGAKIIERHRPENSFFRQHLLKSITTVCPSTTSAATTAFLSGQSPLVT
                     GWFGWQQYFEKYDRHIILFKNEDYYSSETLDTNILQNDLPYENIFEQIKKAKPSVQTY
                     TSIFEDKKKKFLKLNELIRLVKKNFRKDEENFTYAYYADLDSLMHEVGPSHRKAGRLL
                     KRIERRIASLKRRMKDDCLVIVIADHGQIEIEDYNIYDHKELL"
     gene            complement(784..2070)
                     /locus_tag="PLH17_02185"
     CDS             complement(784..2070)
                     /locus_tag="PLH17_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20625.1"
                     /translation="MKISDIVYKSYDEVKAMPRFDFKKRTRKPSNLLKPLMLLLCLPE
                     YLQRKSKVTKINMKGLKPPFFMLCNHNSFFDFKVATMTMFPRVGCNVVAIDGFIGREN
                     LLRNCGCIGKRKFTNDPELIFNIKHILKNKKGFVSIYPEARYSLVGTTSPLPDSLGKM
                     AKMFDVPVVVLNSHGHHLAQPVWNLKKRKCHTESTLTQILTREDLKSLSMDEVNARIQ
                     EAFVYDDYKWQKDNNIHITEDFRAEGLHLPLYKCPICGSEHHMNSKGADLYCESCKTR
                     WHMNELGELECDKKSFSHIPDWFEWQRAQVNKEIRSGKYHYEEEVMIDSLPNATGYYR
                     LGKGKLIHTSEGFRLVGDFAPDFEVAKRPLENFGVHIEYDYFGRGNGVSLSTSGDTYY
                     IYPIDQKQSVTKLHFAAEELYKITKENMNIGKEEII"
     gene            complement(2191..2265)
                     /locus_tag="PLH17_02190"
     tRNA            complement(2191..2265)
                     /locus_tag="PLH17_02190"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2231..2233),aa:Gln,seq:ctg)
     gene            complement(2300..3499)
                     /locus_tag="PLH17_02195"
     CDS             complement(2300..3499)
                     /locus_tag="PLH17_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoylphosphate synthase large subunit"
                     /protein_id="HOR20626.1"
                     /translation="MNFVIISPHFPETYWKFAEALKKNGFTVLGIGDAPYHEIKAELK
                     GVLTEYYYAPKMDDYSELYKAFAFLSSKYGKIDWVESNNEYWLAKDARLRTDFNITTG
                     VMEKDILAYKSKTAMKDYFKKAKVKVARHLKTSDFTVAKKFAKKVGYPLFSKPDIGVG
                     ANSTFRLNNDEDLKTFFLKKDVGEYIIEEYIDGTIVFFDGISNSHHEPVICVEHLFPV
                     TNADVVDGLLEDYYYSLHDVAPDLYKAGCAVLKAFEVNKRFFHLEFFRLNVDKKGLGR
                     KGDLVALEVNMRPPGGYTTDMISACLGVSSYQVYADVVAFDQNRQVANGKPMVVAEVA
                     RRDKYKYELSKEAILAKYQSAMVLSGRYAPLISVGMGDEYFIAKFSTLEEAKIFKEEV
                     LKHQKNI"
     gene            complement(3554..4855)
                     /gene="rimO"
                     /locus_tag="PLH17_02200"
     CDS             complement(3554..4855)
                     /gene="rimO"
                     /locus_tag="PLH17_02200"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOR20627.1"
                     /translation="MLKVGLVSLGCAKNLVDSEIILGMFDDSGYALVNNPDEADIIVV
                     NTCGFIAASKEESIDTIFEMAKYKKKLIVTGCLVQRYYEQLRESLPEADLLVKLEDYP
                     KLHQLIEELNKDGERIAPFNPLRRVISTESYSAYLRISEGCDNRCSFCAIPLIRGPFR
                     SRPYEEVIKEAKILADKGVKELVIISQDTTHYGSDLKESHNIVSLLKSLLEIKEFVSL
                     RLLYLYPDEVTDELIALIRDNPRIAPYFDLPIQHASNRILKRMGRRSSKEKIIALINE
                     IRKEIPHAIMRSTYIVGFPGESEEDFNELVAFTEKVQFNHLGVFTYSKEEDTPSYDFE
                     EQIDEDIKKKRYEIIMETQKKISYRNNRGLVGRVMDGIVTGYDLQSKTYRIRSYFNAP
                     DGIDGNITLTSEKKLNNGDVVKIKILAPFVYDLYAELIEDN"
     gene            complement(4848..>5484)
                     /locus_tag="PLH17_02205"
     CDS             complement(4848..>5484)
                     /locus_tag="PLH17_02205"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOR20628.1"
                     /translation="CGEIYDLIRLDHFRAFDTYYVIDAKEATAERGEWKLGPGRDFFD
                     ELFKHFDATRIIAEDLGDIRPEVLDLRDYYNFPGMNVLQFEILSEKEIAVNPHNVYYI
                     GTHDNDTLKGWYLKLTQDERVQLRKILAKEGLSGDIFAQMVEYALRRPNALSVLFMGD
                     ILKLNNKARINVPGIIDDINWTYRLQSLVTFEEKIKFLRKMIKESNRLINA"
     assembly_gap    5485..5584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5656..6210)
                     /locus_tag="PLH17_02210"
     CDS             complement(5656..6210)
                     /locus_tag="PLH17_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20629.1"
                     /translation="MNKEMTSTKPKTVAIRKRGMVCWLLYAAAVMALAFFGLSFLDSY
                     AVASEVVESTYYSGIELALGVSDLTINYLLLSCLFVGTIGGLLTLIVAIICSLIKKYS
                     PIAAMLMALAIASLTYAFVVSLLFPVVIADSLGASANPLSLMQTMWPIYAMSGLLGGA
                     IIINLSLLIFASVDAYATSGLNRK"
     gene            complement(6207..7112)
                     /locus_tag="PLH17_02215"
     CDS             complement(6207..7112)
                     /locus_tag="PLH17_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20630.1"
                     /translation="MPHHALEVTLRIGVNGLYIAMNAVLFILSVATVLWARKASKDSV
                     HKFIVILLFVNFALHFLKIFLPSYFSDLPYSLKKISPDNICAVSVIFFPFMYLWGGKK
                     FKDYMFYLGIISGFGVYLYPSSYDGYLINNAETVVEIARFYFCHMPLVIAPLAMVASG
                     LHKLSHRRVMWTPIIFLGVLFIVGLNEVFLKLSGITNASWQDVFSNNYRNGALVFGPM
                     SVLDSGLKPFYWMILPFFKYTWPGTDSIYYVPVLWMALPTFIIMSSGYFLISLPFCQR
                     EAYLDYHMIKQKIRMRFRRLGPAKT"
     gene            complement(7088..8275)
                     /locus_tag="PLH17_02220"
     CDS             complement(7088..8275)
                     /locus_tag="PLH17_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOR20631.1"
                     /translation="MLTPQEKTIQEFNRIFENDSTHYFEIGGRLELIGNHTDHNRGKC
                     LVAGCSLGLFASVKKSADNIVHIVANGFDEIYLDLDSLNGRAEEFFTSKAIVRGVAAK
                     LVLSKFKIGGFDAYIESTIFSGAGISSSAAFEVLIARIFDVLYNDGKLTKMQLAEIAH
                     YSETEYFGKPCGLLDQIGVAFGGVNYLDFEDLVNPRVESLDYKLPISIYLVHTGASHA
                     NLTHLYSSIKDDMLFVAKRVFDKKDLRAVSKDDFFKGIAYPTDGVSEQQKLRAQHYID
                     ENDRVDAAREALTKHDAVGFLNAIRQSGFSSSAFLKNTMAGDYNTSPQRGLDLANTVL
                     KDGACRIHGGGFAGTIICFVKDCEEEPFLRVMTDAFGEDHVVKVGIRESGVTHLCLTT
                     PSK"
     gene            complement(8277..9773)
                     /locus_tag="PLH17_02225"
     CDS             complement(8277..9773)
                     /locus_tag="PLH17_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOR20632.1"
                     /translation="MLAILIEKLLKYASCHLYLNDRDLIYVRNLLLRKLRVSAPYEGM
                     IDEEEICRMEVPDVLIEELRKDIQETLKYDDLKTELFIEEIMGDLTPLPSKVIDMFDG
                     LYEVSPEEATAFFYRLCIASNYIRKTSIDKNIVWVSKDGLEISINLSKPEKDNKDIRK
                     LAKVISTKYPKCVLCLENLGYEGRGDHPARETLRVIPLNFDGEGWFWQYSPYGYYDEH
                     LIVVTDEHKPMQVSRANMSKLFSFVDLYPHYFIGSNSDLPITGGSILTHEHFQGGRHV
                     FPMMKAKDAFVIPNSKYPGVKLTYLDFYNSAFRLVGKDEREILDLAMHINHVWQKFED
                     QSAGLRPSSGGERHASLTSIVTKNGEDYTLYLILRNNRCDEIYPDGIFHAHPEHHHIK
                     KEGIGLIEAMGLFILPPRLKRQSALISKILAKDIPADEYLADHPDLEQFVSMINELKK
                     RRGENVEELVRDAISEVCRNILDNTSVFKKDEVGKKALARFIKALEVN"
     gene            complement(9882..11096)
                     /locus_tag="PLH17_02230"
     CDS             complement(9882..11096)
                     /locus_tag="PLH17_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOR20633.1"
                     /translation="MNKFRKFSYRLYQSVLYVASAFLNFREPKVLSGSKSSGKVFGIL
                     VEKNINRVLVITDGPLMKLGLLQKMIDGFEEASIKYAIYSDVVPNPTIDNIEAALELY
                     KKERAKAIIAFGGGSSIDTAKGLAARLANPKKSIEKMRGILKVGGRIPLLIAIPTTAG
                     TGSEATVAAVIVNAQTHSKYAINDPKIIPHYAVLDPDLLVGLPKPITATTGMDAMTHA
                     VEAFIGRANTKNTKCCALSAIQLIDKSLYKSYLEPADLGARKDMQLAAYQAGVAFTRA
                     YVGYVHALAHALGGQYNVPHGLANAILLPYVLEQFGRSVDKKLAILSDSLTLCDKKAS
                     DKTKALAFIQYLKDLNRKLEIPDKFVNVYAEKDIPGLVAHADKEAHPLYPVPSFLDRD
                     ALANVYRRALGL"
     gene            complement(11099..12106)
                     /locus_tag="PLH17_02235"
     CDS             complement(11099..12106)
                     /locus_tag="PLH17_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20634.1"
                     /translation="MAKIKTIYYVCGVVHAQDRVLLVRPLAYNGRPYTPFFFPGTKVA
                     KDDKYEAQKLINAIKLKYKGEVAIDMYMGSSVREFKDKRIVLKAYYCTLKQNFFIPKT
                     KVDYRWAKVEDFDSLRLEPNDREIGERYALFKRVYDGELVAGGRSEKEAAELNFYLDS
                     FEYYGRQLDVKDVKDFNELMRTSAPMEHMRKAYKYVMRINHLDYNSYLNYYEKKNPPN
                     KKELYKPTTDEVDVPDRKTLAELTKGTALHKGEPLNKKARRIKPEIPGKKRSLTGAQV
                     TGIVLLGIALLAEAAILTIALVPFIPDFFATTIGTITLVLSSLLLLVGVMLAFYGRKE
                     E"
     gene            complement(12123..12662)
                     /locus_tag="PLH17_02240"
     CDS             complement(12123..12662)
                     /locus_tag="PLH17_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20635.1"
                     /translation="MANLNPQGKFLISVALVFGIVGVTYLAVGLLNQYHPDPGVRNST
                     DEYGNRVLLCAAEFSDDELDTDWSSRGNEPLFLNKKARFEEDGQNIISPKLNNNFTKY
                     VEFAISTIEGDDAAFIINGFDKDDNLLESAALEDLAVAKIYSHRFAYDHVFIDRITLT
                     YTGQEAVAIVDYVSIYALQ"
     gene            complement(12680..14374)
                     /locus_tag="PLH17_02245"
     CDS             complement(12680..14374)
                     /locus_tag="PLH17_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR20636.1"
                     /translation="MKNNKALPLSEYPRMQLVRNSYLCLNGEWDYVIKNTDDLPKHFL
                     DKIIVPFSPEAPLSKVNRVLKPDEYLIYRRFFDLPNDFNKGRVFLHFTAVDQSAKVYL
                     NNHYLGDHIGGFLPFSFEIQEYLKAENELVVIVQDQSDTSYHSRGKQKLKRGGIWYTP
                     QSGIYLPVWLESTPKEYISSLKITPLFDDKKVEIIAMANIDSDVVIEIEKRVISGMSN
                     TPIIIDLDNMHPWTVDDPYLYKFKVRLKDDEVASYFGMRKISIALDERGKKRFFLNNK
                     PLFQSGLLDQGYYQDGLLTPPSDKDYIDDIMLAKKLGFNMLRKHIKIEAPRWYYHCDR
                     LGILVWQDFVNGGSDYSFLSTVVPVLVQKHSKDNQYKKFGRLDQAGREEFINEARQTI
                     DLLYNSPAVVLWTIFNEGWGQFDAEKVFEEMSKLDPTRLFDHASGWHDQGIGAIKSDH
                     VYFRKYKYEDDKLGRARILSEFGGYQLRVDGHAFNDKNFGYKRMKNSEQLTDAFIRLY
                     EQEIVPAAKEGLAASVYTQLSDVEDELNGLVTYDRQVIKVKAGLIRKINYKLTKGL"
     gene            complement(14371..>14999)
                     /locus_tag="PLH17_02250"
     CDS             complement(14371..>14999)
                     /locus_tag="PLH17_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20637.1"
                     /translation="KANPLLYLTLVSFLAFAIAMQVFMPYFLIVIQKNLHFEGLDFMI
                     IMGSVLLAACLFTVLIGLFMDKIGKNRILMPALGITFVGMIGMFFAKKMAFIIPAGIV
                     MMSGYMISTAVLGSKVRDHTPRNEVGLFQGIRMIFVVLIPMVTGPFIGEASYMFTAAE
                     YINDYGQSVKLPNEYIYLAAAGVLLLAIAPVIILIIKDKKTRALANKK"
     assembly_gap    15000..15099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15100..15446)
                     /locus_tag="PLH17_02255"
     CDS             complement(<15100..15446)
                     /locus_tag="PLH17_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20638.1"
                     /translation="MNKVGVRNWFSLVIAGLVGQFAWTIENMFLNLYIFEQTQDAGYV
                     PWMVAFSAAAATITTLLMGALSDRLGKRKAFVSFGYIAWGLSVLSFAFITTDNMAALF
                     PLANAAMLAGVFI"
     gene            complement(15449..16393)
                     /locus_tag="PLH17_02260"
     CDS             complement(15449..16393)
                     /locus_tag="PLH17_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOR20639.1"
                     /translation="MPAIMTHFAFAKMRAEENVDNDVLYLGAQGPDVFFFYGYSLKKR
                     EHIKDIRGFGTHLHHIDITKAYYYLLGYADKKEGREQEILTRFIKGLFLHYIMDRNCH
                     PFVFYRTGSADEAANKKYYFAAHASYESYIDCLIKERFNLKMAPRKAVKADEGKVVSI
                     SRMMYHLAKDVFKNDIIKEDSYLLAWKDMKFCQIIFHSPLGFKKAVFRLMAPTGAINA
                     MVTPRKVRHNDVLDVLNDQKSEWRDCVTNSSRNDSFLELIAKASNEVDFLEKLLAGKY
                     DEKDLRAFINNIDHDGFQVGAEKKHFAFIWDKVPNMRW"
     gene            complement(16515..16937)
                     /locus_tag="PLH17_02265"
     CDS             complement(16515..16937)
                     /locus_tag="PLH17_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOR20640.1"
                     /translation="MYIIHAAAKETDEACKEDGKNTKGLLLYILFSILTLGIYAFIWS
                     YNIVERWAAFIRKRNQTPRLNGGGMYLLWLIVGSMIGIGPLVAQYLFLHTWNDVNEIH
                     NKEHGGAAAVVKQVAAPKEQITPAVSAGEEAQADDPQE"
     gene            complement(17091..18215)
                     /locus_tag="PLH17_02270"
     CDS             complement(17091..18215)
                     /locus_tag="PLH17_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20641.1"
                     /translation="MITIKEVSTRKMRHQFVSFPLDLYKGNPYFVPPLYGDEMKVFSK
                     KNAYYKTSKSSFFLAYDGDKVVGRISGIIQNQSNEKMGEKRARFTRFDAIDSQEVADA
                     LFAAAERWALANGMESICGPLGYSDLEREGLLIEGFDYIATFEEQYNFEYYGRLIENC
                     GYAKEIDWLEYRLYKPESLDPTLDRVATAAMEKFRLHLGTGRNKRHYINKYKDGIFHC
                     LDEGYKVLYGTVPFTKEMKQQIITQFMYLLSLKFIITVCDEKENVIAFGLSVPSLGPA
                     FQKSGGRLTPGAITKMIKILRKPDVIDLGLIAVLPEYQSFGINAIILRELIIGMMREN
                     ISYCETNLNLENNTKVQAQWKFFPKEQHKKRRSYLKIISR"
     gene            complement(18273..18461)
                     /locus_tag="PLH17_02275"
     CDS             complement(18273..18461)
                     /locus_tag="PLH17_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20642.1"
                     /translation="MQNEKQKKILLSFEQIQDIIGFRLDHSFLNCKKELLAYGYTVGK
                     ISLKEKIITFIKMSDSGE"
     gene            complement(18506..20212)
                     /locus_tag="PLH17_02280"
     CDS             complement(18506..20212)
                     /locus_tag="PLH17_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOR20643.1"
                     /translation="MKKFHLTDTYALINFDASLCDSEVSVLQSKSFAHVLSQFVDKLR
                     RDKDVTMESLKTVKLEHFLDAYKLLYVFPFNDIYKNNPSLHYLLKKRDEFYHFTEMFY
                     DYWRSLQRFGLMASSHLYTQSAKAPDVITTIDHFNSLILSLYRGVSTNLLGQQFHVYR
                     QLPAGVNANLLYVHHRVPYHAGYENLQNIAFISKIATRPPFIAYTKSNTRSGLFQEIG
                     ENPLSKLVINKLHFFAFPIKIGPLLAYVYIHRDFLHEGIGLSNLFEFASFDEIKDRKP
                     DIVYLYGISETEYDCTYHHDQENNIYLGFVSRLDKNDYFGYLKKMLLTLHNLAMIDQK
                     KLPIHGAMVSIVLNNDETKNIVVVGDSGAGKSETLEALRIIGKDYIKQMKIVFDDMGT
                     FFKKDGHIYAHGTEIGAFVRLDDLEAGYAYQKIDRAIFLNPDQVNARVVLPVTTYKFI
                     MADHRIDMLLYANNYSEVDIGLRLFSNLEEATDIFRGGQRKAKGTTSEVGLVSSYFAN
                     PFGPVQRKEQTEVLLNSYFTSLYKEKVAIGELFTRLAVPGYEMAGPQEAATKLLKSLI
                     GK"
     gene            complement(20223..21347)
                     /locus_tag="PLH17_02285"
     CDS             complement(20223..21347)
                     /locus_tag="PLH17_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="HOR20644.1"
                     /translation="MNIKRNILLNPGPATTTDTVKAQLMAPDMCHREQDFVDVIEKLR
                     KDLVKIAHGNDKDHVAVLFSGSGTINMDVALNSLLPDGKKVLIVNNGAYSSRAVDICK
                     YYGLSYIELKLDFTAPIEPGKIKEVLNDNPDITLVYTTHHETGSGILNDIRSIGKIVH
                     EHQAVFVVDATSSFALVPLDIVKDNVDFLLASAQKGIAAMPGLSFVIGRRDLIELSKD
                     YPKRSYYCHLYRQYHHFEVDGEMQFTPPVQLVYAAAQGVKEYFAEGENDKYKRIVRVN
                     QAIHEGLKRLGFKEYIKREHQGPLVVSVLYPNDVNWNFKKVHDFCYERGFILFPRPVG
                     GVLAFRLGSLGAIDVGDIDNFFKVLKDALLSLKIEVPVRY"
     gene            complement(21971..22046)
                     /locus_tag="PLH17_02290"
     tRNA            complement(21971..22046)
                     /locus_tag="PLH17_02290"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22011..22013),aa:Phe,seq:gaa)
     gene            complement(22054..22129)
                     /locus_tag="PLH17_02295"
     tRNA            complement(22054..22129)
                     /locus_tag="PLH17_02295"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22094..22096),aa:Asp,seq:gtc)
     gene            complement(22237..22779)
                     /locus_tag="PLH17_02300"
     CDS             complement(22237..22779)
                     /locus_tag="PLH17_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOR20645.1"
                     /translation="MKELKGTRTEENLKIAFSGESQARVKYGYYAKQAKKEGYEQIAA
                     IFEETSDNEKEHAKLWFKALHGGSVPTTAENLLDAATGENYEWTTMYAEMAKVAKEEG
                     FPEIARMMLGVANVEKEHEKRYRHLLKKVTDEKIFECDEVTVWKCRNCGHIHIGKKAP
                     EICPVCAHPKAHFELKAENY"
     gene            complement(22852..23670)
                     /locus_tag="PLH17_02305"
     CDS             complement(22852..23670)
                     /locus_tag="PLH17_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR20646.1"
                     /translation="MKRKRENPKRIIYYRDLLNDDFAGTKIKTQPLDENFRYIHKNIL
                     WRILSFLVYFLVAIPVLFLTCKIALNVKVVNRKAIKNLRKTGYFIYGNHTHIMDAFIS
                     QVCITRPKRTFIIADPDAVSIPGVRNLVMMLGCLPLPSTNGTTKKYLEAIQYHIGKKH
                     AIVVFPEAHIWPFATFIRPFSSSSFRYPAKLNTPVVPIVATYEKRKIFKRCLKPRMVV
                     TVGEPIFPKRDTDLKTSMQDLHDQTYNFMVEHASKPDNVEYFRYVKSDDEKSEN"
     gene            complement(23645..24799)
                     /locus_tag="PLH17_02310"
     CDS             complement(23645..24799)
                     /locus_tag="PLH17_02310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR20647.1"
                     /translation="MRVTIVADVLGIENNGTTITAVNFINSLKNKGHEVSVVCPDENK
                     TGLYGYYVVPPLNLGFLNNYVKKNNITLAKADSKIITDAIKNADIVHVMMPFFLGTKA
                     ARIAKRLGKPVTAGFHVQAENFTNHIFMMDMPVANLLTYKFFYRVLYRYVDAIHYPTQ
                     FIREVFEHHAGETNGYVISNGVHKAFKPRLAPKPKNLDGKFVILFTGRYSREKSHKVL
                     IDAVKLSKYKDRIQLVFAGDGPQRDNIIKRSADLANAPILGFHPREELIDIINYADLY
                     VHPAEIEIEAISCLEAMSCGLVPIIADSRRSATRHFALSEKNLFKCNDAKDLAEKIDY
                     WLDSPDEKERCRTQYIEYAKRFDFDYSMEQMESMLFDIIKANDEKKTRKS"
     gene            complement(24911..25558)
                     /locus_tag="PLH17_02315"
     CDS             complement(24911..25558)
                     /locus_tag="PLH17_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20648.1"
                     /translation="MDKKVIKERHKRFSERIEALYRDSRSATKKYVESLTYPDGKAHI
                     SVDLTNVDELYSPYSYKEDMKPDIFEYIDEKTYYTPVEIPLIVNFDVGDVSDLRKSRI
                     KKSFLDHYRLIYEDKRRELKINRAVAWVLFAIGMIALVISTIAKLAMNAAQFPDIPLI
                     FEMLQIFSWVFLWEATAKFFFQGREMRIQCMNAGQLALAEVKFNEPEPELKDNNM"
     gene            complement(25551..>25877)
                     /locus_tag="PLH17_02320"
     CDS             complement(25551..>25877)
                     /locus_tag="PLH17_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20649.1"
                     /translation="YHSDKTCRLFSTYFYDKVSLGQNMKLRIKQLVSLKEANQNFSRV
                     AWKVDEEGYVLLLRNNKPAYLVFAYDEDDIVADDSSFLEALHHLIERKRAAKLKKKIK
                     GGAENG"
BASE COUNT         6619 a         5587 c         6307 g         7164 t
ORIGIN      
        1 agcaattctt tatggtcata aatattatag tcttcaatct cgatttgtcc gtgatcagcg
       61 atgactatta ctaagcagtc atccttcatt cttcttttca aagaagcgat gcgcctttca
      121 atgcgtttta gaagtctccc cgcttttcga tgcgagggtc cgacttcgtg catcaaagaa
      181 tcgaggtcgg cgtaataggc ataagtgaag ttctcttcgt ctttgcggaa gtttttctta
      241 actaatctaa tcagttcgtt aagctttaaa aacttctttt tcttatcttc gaaaattgat
      301 gtatacgttt gaacgcttgg tttagccttc ttaatctgct caaagatgtt ttcgtaaggt
      361 aagtcatttt gaagaatatt cgtatcaaga gtctcgctgc tataatagtc ttcgttttta
      421 aacaagatga tatggcgatc atatttttca aagtattgct gccagccaaa ccagccggtc
      481 accaatggtg attgccccga taagaaagcc gtcgtcgccg cggaagtcgt cgaggggcac
      541 accgttgtaa tgctcttaag caagtgctgc cggaaaaagg agttttccgg gcgatgtctt
      601 tcgatgatct ttgcccccat cgcatcgatt aaaaagacgg cgatgtggcg gtatttcttg
      661 tttaaaatag cgtctaattc tttctgacca ctatagttcg tctttgcacc ataatgctta
      721 agcaaggaat tagagacatt aagaatcgag tcttgataat taggaagaac gactttgctc
      781 atactagatg atttcttctt taccgatatt catgttctct ttggtaatct tgtatagttc
      841 ttcggcggcg aagtgtaact tcgtcaccga ctgcttctga tcgatgggat agatgtaata
      901 cgtatcgccg ctcgtcgaaa ggctgacgcc gttacctctt ccgaagtaat cgtattcgat
      961 gtggacgccg aagttttcta aaggccgctt ggcgacttca aaatcagggg cgaaatcacc
     1021 gacgaggcgg aagccttcgc tcgtatgaat tagcttgcct ttgcccaggc ggtaatagcc
     1081 ggtcgcgttc ggaagggaat caatcatcac ttcttcttcg taatggtatt tgccggagcg
     1141 aatctcttta ttgacctggg ctctttgcca ttcaaaccag tcgggaatat ggctaaacga
     1201 tttcttgtcg cattcgagct cgccgagctc attcatatgc catctcgtct tacagctctc
     1261 gcaatataaa tccgcgcctt tggagttcat gtggtgttcg gaaccgcaga tggggcactt
     1321 atataaggga aggtgcagtc cttcggctct gaaatcttcg gtgatatgga tgttattatc
     1381 tttttgccac ttatagtcgt catagacgaa agcttcctga atgcgggcgt tgacctcatc
     1441 catcgaaagg ctctttaaat cctcgcgcgt cagaatctgc gtcagcgtcg attccgtatg
     1501 gcactttctt tttttaagat tccacaccgg ctgggcgagg tgatggccgt ggctatttaa
     1561 gaccaccacc ggcacgtcga acatcttggc catcttgcct aaagaatcag gaaggggcga
     1621 agtcgtcccg acgagggaat aacgggcttc gggataaatc gaaacgaagc cttttttgtt
     1681 tttgagaatg tgtttgatat taaagattag ttcgggatca ttggtgaact tgcgcttgcc
     1741 gatgcagccg cagttcctga ggagattttc gcggccgata aaaccgtcga tggccacgac
     1801 attgcagccg acgcggggaa acatcgtcat cgtcgcgact ttaaagtcga aaaacgaatt
     1861 gtggttgcaa agcataaaga aaggcggttt gagtcctttc atattaatct tggtgacttt
     1921 gctctttctt tgtaagtatt cgggcaggca aagcagcaac atcaacggct tgagcaaatt
     1981 gctcggttta cgcgttcttt ttttaaaatc aaagcgcggc atggctttaa cttcgtcgta
     2041 gcttttatat acgatgtcag aaattttcat ttattcgttc tccgatgaat tcttattcat
     2101 tataccccag ccccttaaaa atacaatctc ggctctttgt cacgataaac gaaaaagaaa
     2161 acgaccgctt ttgcgatcgt ttgtaagcgt tggctgccga gaaaaggttc gaacttttgc
     2221 atacgcggtt cagagccgcg tgccttacca cttggctact cggcaatgct tatatattat
     2281 gtcgcattta agacataact caaatatttt tttggtgttt tagcacttcc tctttaaaaa
     2341 ttttagcttc ttctaaggtt gaaaatttag cgatgaaata ctcatcaccc atgccgacgc
     2401 tgatcaacgg cgcatagcgg ccgcttaaaa ccatcgcgct ttgatacttg gcaagaatgg
     2461 cttctttgct taattcgtat ttatatttat ctcgcctggc gacttccgcc accaccatcg
     2521 gttttccgtt cgccacttga cggttttgat caaaagcaac gacatccgca tacacttgat
     2581 aagaactcac tccgaggcag gcggaaatca tgtccgtcgt atagcctccc ggtggccgca
     2641 tattgacttc gagggccact aaatcacctt tgcggcctaa gcccttctta tcgacattaa
     2701 ggcgaaagaa ttcaagatga aagaagcgct tattaacctc aaaggctttt aacaccgcgc
     2761 atccggcttt gtataagtca ggggcgacat cgtggagcga gtagtaataa tcttcgagga
     2821 gaccgtcgac gacatcggcg ttcgtcacgg gaaaaagatg ctcgacacaa atgacgggtt
     2881 cgtgatgaga gttgctgatg ccgtcaaaga agacgatcgt cccgtcgata tattcctcga
     2941 tgatgtattc gccgacatct ttctttaaaa agaaagtttt gaggtcctcg tcattattaa
     3001 gccggaaagt cgagttcgcc ccgacgccga tatcgggctt tgaaaataag gggtagccaa
     3061 cctttttggc aaacttttta gccaccgtaa aatcacttgt cttgaggtgg cgggctactt
     3121 tcaccttggc ctttttaaaa taatctttca tcgccgtctt tgacttgtag gcgagaatat
     3181 ctttttccat aacgccggtc gtgatgttga agtcggttct tagtctcgca tcttttgcga
     3241 gccagtattc attattcgat tcgacccagt cgatcttgcc gtatttcgaa cttaaaaacg
     3301 caaacgcttt atagagttca ctataatcat ccattttcgg ggcgtaataa tactccgtca
     3361 aaacgccttt gagttcggct ttgatttcgt gataaggagc gtcgccgatg cctaagacgg
     3421 tgaagccgtt tttctttaag gcttcggcaa acttccagta cgtctcggga aagtggggcg
     3481 agatgatgac aaaattcatg cggatgacaa cctcttaaaa tattatagtc aaaattgttc
     3541 tattaaaaat agattaattg tcttcgatca gttcggcgta caaatcataa acaaaaggag
     3601 cgaggatttt aattttgacg acatcgccgt tatttagttt cttttcgctt gttaatgtga
     3661 tgttgccgtc gattccgtcc ggggcgttaa aataagagcg aatgcgatat gtcttacttt
     3721 gtaagtcata gccggtgacg atgccgtcca tcacccttcc gactaaaccg cggttattgc
     3781 ggtaggagat cttcttctgc gtctccatga taatttcgta gcgcttcttt ttgatgtctt
     3841 cgtcgatctg ctcttcgaag tcatatgagg gcgtatcttc ttctttagag taggtaaaga
     3901 cacccaagtg gttaaactga actttctcgg taaaagccac caactcgtta aagtcttctt
     3961 cgctttcgcc gggaaagccg acgatataag tcgaacgcat tatggcgtgc ggtatctctt
     4021 tgcgaatctc gttgatcagc gcgatgatct tttctttgct gctccgcctt cccatccgct
     4081 tcagaatgcg gttcgaagcg tgctgaatcg gcaggtcaaa ataaggggcg atgcgcggat
     4141 tatcgcggat taaagcaatc agctcatcag ttacttcgtc aggataaaga taaaggaggc
     4201 gcagggaaac aaattcttta atttcgagca agctctttaa taaagagaca atattatgac
     4261 tttctttcaa atcgcttccg taatgggtcg tgtcctgcga gatgatgact aattccttaa
     4321 cgcctttatc ggcgaggatt ttcgcttctt tgatcacctc ttcataaggc cgtgagcgaa
     4381 acggtccgcg aataagagga atcgcgcaga aggagcagcg attatcgcag ccttccgaga
     4441 tgcgaagata ggcgctatag ctctcggtgg agatgacgcg ccgtaacgga ttaaaggggg
     4501 cgatacgttc gccgtcttta ttgagttctt caatcagttg atggagtttt ggataatctt
     4561 cgagtttgac gagcaaatcg gcttcgggaa gcgattccct aagctgctca taataccttt
     4621 ggacgaggca tccggtaaca atcaatttct ttttgtattt ggccatctcg aaaatggtgt
     4681 cgatgctctc ctctttgctc gcggcgatga acccgcaggt gttgacgacg atgatatcgg
     4741 cttcatcagg attgttaacc aaagcgtaac cggaatcatc aaacatgcct aaaatgattt
     4801 cgctatcaac gagatttttc gcgcatccta aggaaactaa gccgacttta agcattaatc
     4861 aatctgttac tctctttaat catcttgcga agaaatttaa tcttttcttc gaaagtcact
     4921 aaagattgta agcgataagt ccagttgatg tcatcaataa tgcccgggac gttgatgcgg
     4981 gctttattat tgagcttcaa gatatcgccc ataaagagga cggagagggc gttcggccgc
     5041 cttaaggcat attccaccat ctgcgcgaat atatcgcctg agaggccttc tttggccaga
     5101 atcttgcgca attgtacgcg ctcatcttga gtgagcttta aataccagcc ttttaaggtg
     5161 tcgttatcgt gcgtcccgat gtagtagacg ttatgggggt tcaccgctat ttctttttcg
     5221 ctcaggatct caaactgcag cacattcatg cccgggaaat tatagtagtc tcttaaatcg
     5281 aggacctcgg ggcgaatatc acctagatcc tcggcgataa ttcttgtcgc atcaaaatgc
     5341 ttaaagagtt catcgaagaa atcgcggccg gggccgagtt tccactcgcc gcgttccgcc
     5401 gtcgcttctt tcgcgtcgat gacgtagtag gtatcaaacg cccgaaagtg atcgaggcga
     5461 atcaaatcat agatttcgcc gcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnngaaaca ggcatgagca aaccggcttg acgggcgtgt gtaaagcgag cattttccat
     5641 aggcgtttct cctctttatt ttctatttaa gcctgaggtg gcataagcat caacgctggc
     5701 gaagatgagg agcgaaagat tgatgatgat cgctccgccg aggaggccgc tcatcgcgta
     5761 gatcggccac atcgtctgca tcagcgatag cggattggcg gacgcgccga gcgaatcggc
     5821 gatgacaacc ggaaagagca gcgagacgac aaaggcgtaa gttaatgagg cgatggctaa
     5881 agccataagc atcgccgcaa tcggcgaata cttcttgata agcgagcaga tgatggcgac
     5941 gataagcgtt aaaagaccgc cgatcgtccc gacaaaaaga caggataaaa gcaagtagtt
     6001 tatcgtcagg tcggagacgc ccaaagcgag ttcgatgccc gagtaatacg tgctttcgac
     6061 gacttcgctg gcgacagcat aggaatcaag aaacgaaaga ccaaaaaagg ctaacgccat
     6121 aacggcggcc gcgtataaga gccaacagac cattccgcgt ttgcggatgg cgacggtctt
     6181 gggttttgtg gatgtcattt ctttattcat gtcttggcag gtcctaagcg tctaaaacgc
     6241 attctgatct tctgctttat catgtgataa tcaaggtacg cctcgcgctg acaaaatgga
     6301 agcgaaatga ggaaatatcc gctgctcatg atgataaaag tcggaagcgc catccagagg
     6361 accgggacat aatagatgct atccgtgccc ggccaggtgt atttaaaaaa tggcagaatc
     6421 atccagtaaa atggctttaa tccggagtcg aggacgctca tcggtccgaa gacgagagca
     6481 ccgttgcggt agttgttgct gaagacatcc tgccagctcg cgttggtgat gccgctcagt
     6541 tttaaaaaga cttcgtttaa gccgacgata aataagactc caaggaaaat gattggtgtc
     6601 cacatcacgc ggcgatgact cagtttatga aggccgcttg ccaccatcgc taacggggcg
     6661 atgaccagcg gcatgtggca gaagtaaaag cgggcgattt cgacgaccgt ctcggcgtta
     6721 ttaattaaat agccatcata agatgacgga tataaataga cgccgaagcc gctgatgatg
     6781 ccgagataaa acatgtagtc tttaaacttc ttgccccccc ataaatacat aaagggaaag
     6841 aagatgaccg agacggcgca gatgttgtcg ggcgatatct tttttagcga gtatggtaaa
     6901 tcgctaaaat acgagggtaa aaagattttt aaaaagtgca gggcaaaatt aacaaaaagc
     6961 aagatgacga taaatttatg aacgctatct ttgctggctt tgcgcgccca aagaaccgtc
     7021 gcgacggaaa ggataaacaa aacggcgttc atcgcgatat ataaaccgtt gacgccgatt
     7081 cgtaaggtta cttcgagggc gtggtgaggc aaaggtgggt gactcctgat tcgcgaatgc
     7141 cgactttgac gacatggtct tcgccaaagg cgtcagtcat aactcttaaa aagggctctt
     7201 cttcgcaatc tttgacaaag cagataatcg tgccggcaaa gcccccgccg tgaatgcggc
     7261 aggctccgtc ttttaagacg gtgttggcga ggtcaagacc tcgttgcggc gaagtattat
     7321 aatcgccggc catcgtgttc tttaaaaagg ccgaactgga aaatcccgat tggcgaatgg
     7381 catttaaaaa gccgacggca tcgtgttttg tcaaagcctc gcgggcggcg tcgacgcggt
     7441 cgttctcatc gatgtagtgt tgggctctta gcttttgctg ctcacttacg ccatcggtgg
     7501 gataggcgat gcctttaaag aaatcatctt tgctgacagc gcgcaggtct ttcttatcga
     7561 agacgcgctt ggcgacaaag agcatgtcgt ctttaatcga agaatagaga tgcgtcaaat
     7621 tggcgtggct cgctcccgtg tggacgagat agatgctgat tggcagttta taatcgaggc
     7681 tctcgacgcg gggattaact aagtcttcaa agtcgagata gttgacgcct ccaaaggcga
     7741 cgccgatctg gtcgagaaga ccgcagggtt ttccgaagta ttcggtttcg ctgtagtggg
     7801 cgatttcggc tagttgcatt ttcgtgagtt tgccatcgtt atataaaaca tcaaagatgc
     7861 gggcgatcaa gacttcaaaa gccgcacttg aggaaatgcc ggcacccgaa aagatcgtcg
     7921 attcgatata ggcgtcaaaa ccgccgattt taaacttgct taaaaccagc ttggcggcca
     7981 cgccgcggac gatggctttc gaggtgaaaa attcctcggc ccggccgttg aggctatcga
     8041 ggtcaaggta gatttcgtca aagccgttgg cgacgatgtg gacgatgttg tccgcgcttt
     8101 tctttacgct cgcaaaaagg cctaaactgc agccggcgac taagcacttg ccgcggttgt
     8161 ggtcggtgtg gttgccgatt aattctaagc gcccgccgat ttcaaaatag tgggtcgaat
     8221 cattttcaaa gatgcggtta aattcctgaa tcgtcttctc ttggggagtc agcatgttaa
     8281 ttaacctcca gcgccttgat gaagcgggcc aaagctttct ttcctacttc gtctttttta
     8341 aagacggatg tgttgtctaa aatgtttcta cacacttcag agatggcgtc gcggacgagt
     8401 tcttcgacgt tttcgccgcg ccttttttta agttcgttaa tcatcgagac gaactgctcc
     8461 aagtcggggt gatcggcgag gtattcatcg gcgggaatat ctttggcaag gatcttgctg
     8521 attaaggctg attgtcgctt gaggcggggc ggaaggataa ataaacccat cgcctcgatc
     8581 aggccgatgc cctctttttt gatgtgatga tgctcgggat gagcgtgaaa gatgccgtcg
     8641 ggataaatct catcgcagcg gttgttgcga agaatcaaat ataaggtgta gtcctcgcca
     8701 tttttagtga cgatggatgt gagcgaggca tggcgttcac cgccgctgct ggggcggagg
     8761 ccggcgcttt gatcttcaaa tttttgccag acatgattga tgtgcatcgc taagtcgagg
     8821 atttccctct catctttacc gactaagcga aaagccgagt tataaaaatc gaggtaggta
     8881 agtttgacgc ctggatattt gctgtttgga atcacaaagg catctttggc cttcatcatc
     8941 ggaaagacgt ggcggccgcc ctgaaagtgc tcgtgggtta agatggagcc gccggtgatc
     9001 ggcagatcgg aattcgaacc gatgaagtaa tggggatata aatcgacaaa cgagaacagt
     9061 ttactcatat tcgcgcggct cacttgcatc ggtttgtgct cgtcggtgac gacgattaga
     9121 tgttcgtcgt aatagccgta gggcgaatac tgccagaacc agccttcacc atcaaaattc
     9181 aaggggataa cacggagtgt ttcgcgggcc ggatggtcgc ctcttccctc gtagccgagg
     9241 ttttctagac agaggacgca cttgggatat ttggtgctga tgactttggc gagtttacga
     9301 atatctttgt tatctttttc gggtttggaa aggttgatcg aaatttctaa tccatctttg
     9361 gaaacccaga cgatattttt atcgatgctc gtcttacgga tgtaattcga ggcgatgcag
     9421 agacggtaga aaaaggccgt cgcttcctcg ggtgaaactt cgtataagcc atcgaacata
     9481 tcgattactt tcgagggcag gggcgtcagg tcacccatga tttcctcgat gaacaattcg
     9541 gttttgagat catcgtattt aagtgtttcc tgaatgtctt tgcgaagttc ttcaatgagg
     9601 acatcgggga cttccatgcg gcaaatctcc tcttcgtcga tcattccttc gtaaggagcg
     9661 ctgactctga gtttgcgaag caggaggtta cgaacataaa ttaagtcgcg atcatttaaa
     9721 tataaatgac aggatgcata ctttaatagt ttttcgatta atatggcgag cataaaacaa
     9781 ttccttttcg catatattat atagtaagtc tttcgcaaat gttatagccc gcaataaaag
     9841 gatgtctttt atggcatctt atattcgggc ggacaccgca cttataagcc gagggcccgg
     9901 cggtagacgt tagctaaagc gtcgcgatca agaaaagacg ggacgggata gagaggatgg
     9961 gcctctttat cggcgtgagc gacgagtccc ggaatatctt tttcggcata gacattgacg
    10021 aacttgtcag ggatttccag ttttctgttg aggtctttaa gatattggat aaaggctaaa
    10081 gccttggttt tgtcgctggc ttttttgtcg cataaagtga gagaatcgct taagattgcc
    10141 aacttcttgt cgaccgaacg gccgaattgc tccaagacat aagggagcag gatagcattg
    10201 gccaggccgt gggggacatt atattgcccg cctaacgcgt gagctaaggc atggacgtag
    10261 ccgacatagg ctctggtaaa ggcgacgccg gcctgatagg cggctaactg catgtctttg
    10321 cgcgctccca aatcagcggg ttcaagatag gatttataga ggcttttatc gatgagctga
    10381 atggctgaaa gggcgcagca cttcgtattt ttcgtattcg cccgaccgat gaaggcttcg
    10441 acggcgtggg tcatggcgtc catgccggtc gtcgcggtga tgggtttggg aagtccgact
    10501 aataaatcgg gatcaaggac ggcataatgg gggataatct tgggatcgtt aatcgcgtat
    10561 ttggagtggg tctgggcgtt gacgatcacc gccgccaccg tcgcttcgct gcctgttccg
    10621 gctgtcgtcg ggatagcgat taaaagcgga atgcggcctc cgacttttaa gatgccgcgc
    10681 atcttttcga tgctcttttt gggattggcg aggcgagcgg ccaagccctt cgccgtgtcg
    10741 atgctcgagc cgccgccaaa agcgatgata gctttagccc gctctttttt gtagagttct
    10801 aaagccgctt cgatattgtc gatcgtcgga ttgggaacga catcggaata gatagcgtat
    10861 ttgatgctgg cttcttcaaa gccatcgatc atcttctgca aaagccccaa cttcatcagc
    10921 gggccatcgg tgatgacgag aacgcggtta atattctttt cgacgaggat gccgaacacc
    10981 ttgcccgacg actttgagcc gctgagcact ttgggttcgc gaaagtttaa aaaagcgctg
    11041 gcgacataaa ggaccgattg atataaccgg taagaaaact ttctaaattt attcatattt
    11101 attcctcttt tcgaccgtag aaggcgagca tgacgccgac caagagaagc agtgacgaga
    11161 ggacgagggt aatcgtgccg atcgtcgtcg caaagaaatc ggggatgaag ggaaccaagg
    11221 caatcgtcag gatggcggct tccgccagca aggcgatgcc gagcaagacg atgccggtga
    11281 cctgagcacc ggtcagggag cgcttcttgc cgggaatttc gggtttgatg cgcctggctt
    11341 ttttgtttaa gggctcgcct ttatgcaaag cggtgccttt tgtcaactcg gccaacgtct
    11401 tgcggtcggg gacatcgacc tcgtcggtcg tcggtttata aagttccttt ttattcggag
    11461 gatttttctt ttcgtagtag tttagataag aattgtaatc gaggtggtta atccgcatga
    11521 catacttgta ggctttccgc atatgctcca taggagcact cgtccgcatc aactcattaa
    11581 aatccttgac atctttgaca tcgagctgac ggccgtagta ttcgaaggaa tcgagataga
    11641 aattgagttc ggcggcttcc ttttcgcttc ttcctccggc gactaattca ccgtcgtaaa
    11701 cgcgtttgaa taaggcgtaa cgctcgccga tttcacgatc gtttggctcg agtctaagcg
    11761 agtcgaaatc ttcgactttg gcccaccggt aatcgacttt cgttttgggg ataaaaaagt
    11821 tttgttttaa ggtgcagtaa taagctttta aaacgatgcg cttatcttta aattcgcgga
    11881 ctgaggagcc catgtacata tcgatggcga cctcgccttt atatttgagt ttgatggcgt
    11941 tgatgagctt ttgcgcttcg tatttatcgt cctttgccac tttggtgcca ggaaagaaaa
    12001 aaggcgtata aggccggccg ttataggcca ggggacgaac gagcaagacg cggtcttggg
    12061 cgtgaacgac accgcagacg taatagattg ttttgatttt tgccatggtg attctctttt
    12121 atttattgta atgcatatat gcttacataa tcaacgatag cgacggcttc ctggccggtg
    12181 taagttaagg tgatgcggtc gataaaaacg tgatcatagg cgaagcgatg gctgtagatc
    12241 ttggcgacgg cgagatcttc aagagcagcg ctttctaaga gattatcatc cttgtcaaag
    12301 ccattgatga taaaggcggc atcatcccct tcaatcgtgc tgatggcaaa ctcgacatac
    12361 tttgtaaagt tattgttcag cttcggcgaa atgatgttct ggccgtcctc ttcaaagcga
    12421 gctttcttat taagaaagag tggctcattt ccgcggctcg accagtcagt gtctagttca
    12481 tcgtcgctaa attccgcggc gcataaaagc actcggtttc cgtactcgtc cgtgctattc
    12541 cgcacaccgg ggtcagggtg gtattgatta agaaggccga ccgctagata tgtcacgccg
    12601 acgatgccaa aaacaagagc gacggagatc agaaatttgc cttgcggatt aagatttgcc
    12661 atatctctac tataaggaat tagagccctt ttgttaattt ataattaatt ttgcgaatta
    12721 atccggcttt gactttgatg acttggcggt cgtaggtaac aagtccattc agttcgtctt
    12781 cgacatcgct taactgcgtg tagacggagg ccgctaatcc ctccttggcc gcgggcacga
    12841 tttcctgttc gtagagacgg atgaaggcgt ccgttaactg ctcgctattc ttcatccgtt
    12901 tatagccgaa attcttgtcg ttaaaagcat ggccgtcaac gcgcagttga tagccgccga
    12961 actccgataa gatgcgggct cgtccgagct tatcgtcttc atatttgtac ttgcgaaaat
    13021 agacgtgatc ggatttgatg gcgccgatgc cctgatcatg ccatcccgag gcgtgatcaa
    13081 agagtctggt cggatcgagt ttgctcatct cttcgaagac tttttcggca tcgaactgcc
    13141 cccagccttc gttaaagatt gtccaaagga caacagcggg tgaattataa agtaaatcga
    13201 tcgtttgtct agcttcgttt ataaactctt cgcggccggc ttggtcaagc cgtccgaact
    13261 ttttgtattg attgtctttg ctgtgctttt gcacaaggac gggcacgacg gttgaaagga
    13321 aggagtaatc gctgccgcca ttgacgaaat cctgccagac gaggatgcct aaacggtcgc
    13381 agtgataata ccagcgcggc gcttcgattt tgatgtgttt gcggagcatg ttaaagccga
    13441 gttttttggc cagcataatg tcatcgatat agtctttgtc ggaaggcggg gtgaggaggc
    13501 cgtcttgata gtagccctga tcgaggagac cgctttgaaa taagggcttg ttgtttagga
    13561 agaagcgctt cttgcctcgc tcatcaaggg cgatggaaat cttgcgcatg ccaaagtaag
    13621 aggcgacttc atcatctttc aaccgcactt tgaatttgta aaggtaagga tcgtcgacgg
    13681 tccacggatg catgttatcg agatcgatga taatcggcgt attgctcatg cctgagatca
    13741 cccttttttc aatttctatg acgacatcgc tatcgatgtt cgccatagcg atgatttcga
    13801 ctttcttatc gtcaaatagc ggcgtgattt ttaaagaaga aatatactcc ttcggcgtgc
    13861 tttccagcca caccggcaga taaattccgc tttgcggagt gtaccagata ccgccgcgtt
    13921 taagcttttg cttaccgcgg ctgtgatatg aggtatcgct ttgatcttgg acgatgacga
    13981 cgagctcgtt ttcggccttt agatattcct gtatttcaaa cgaaaaaggc aggaaaccgc
    14041 cgatatgatc accgagataa tgattgttta aataaacctt cgcgctttgg tcgacggccg
    14101 taaaatgaag aaagacgcga cctttattaa aatcattagg aaggtcaaaa aagcgtcggt
    14161 aaataagata ttcgtccggc tttaaaacgc ggttgacttt tgaaaggggg gcttcgggtg
    14221 agaacggcac gatgatcttg tctaaaaaat gcttgggaag atcatcggtg ttcttgatga
    14281 catagtccca ttcgccattc aggcaaagat aggaattgcg cacaagttgc atgcgcggat
    14341 attcgctgag cggaagtgct ttgttgtttt tcatttttta tttgcaagag cgcgagtttt
    14401 cttatctttt attattaaaa taataaccgg ggcgatcgct aagagtaaga cgcccgctgc
    14461 cgcgaggtat atgtattcat tgggtaactt gactgattgg ccgtagtcat ttatgtactc
    14521 ggccgcggta aacatgtaag aggcttcgcc gataaaaggt ccggtcacca tcggaatcag
    14581 gacgacgaaa atcatgcgga taccctgaaa gagaccgacc tcatttcgcg gagtgtgatc
    14641 gcggactttg gagccgagca ccgccgtcga aatcatatag cccgacatca tcacgatgcc
    14701 ggccggaata ataaatgcca tcttcttagc gaagaacatg ccgatcatgc cgacgaacgt
    14761 gatgcctaaa gccggcatga gaatgcggtt tttgccgatc ttatccataa aaagaccgat
    14821 gaggacggta aaaaggcagg cggctaataa tacgctgccc atgataatca tgaaatcgag
    14881 gccttcaaag tgaagattct tttgaattac aatcaagaaa taaggcataa acacctgcat
    14941 ggcaatggcg aacgccaaaa atgaaacaag ggttaaataa aggagcggat tggctttgan
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgataaaaac gccggcgagc
    15121 atcgcggcgt tcgccagcgg aaataatgcc gccatgttat cggtcgtgat aaaggcaaat
    15181 gataagaccg agaggcccca ggcgatataa ccaaaggaaa caaaggcttt gcgctttccg
    15241 agacgatcgg ataaggcgcc cataagcagc gtcgtaatcg tcgccgcggc ggcgctaaaa
    15301 gcgaccatcc acgggacgta accggcatcc tgtgtctgct cgaaaatata gaggttaaga
    15361 aacatatttt caatcgtcca ggcaaactgg ccgaccaaac cggcgatgac gagcgaaaac
    15421 caattgcgaa cacctacttt attcatcgtt accacctcat gttgggcact ttgtcccaaa
    15481 taaatgcaaa atgcttcttt tcggccccga cttgaaagcc gtcgtgatcg atattgttaa
    15541 tgaaggcgcg aaggtctttt tcgtcgtact tgccagcgag taatttttcc aaaaagtcga
    15601 cctcatttga agctttagca atcaactcta aaaacgaatc attgcgagat gagttggtga
    15661 cgcagtcgcg ccactcgctc ttttgatcgt ttaagacatc caagacatcg ttgtgcctga
    15721 ccttgcgggg tgtcaccatc gcgttgatcg ccccagtcgg ggccatcagg cgaaagacgg
    15781 ctttcttaaa tcctaaaggc gaatgaaaaa ttatctggca aaacttcatg tccttccagg
    15841 cgagaaggta tgaatcttcc ttgatgatgt cgtttttaaa tacgtccttg gcaaggtgat
    15901 acatcatgcg agagatggaa acgactttgc cctcgtcggc tttaaccgcc ttgcgcggtg
    15961 ccattttgag attaaaacgc tctttgatta ggcagtcgat gtacgattca taagaggcgt
    16021 gggcggcaaa gtaatatttc ttgttcgcgg cctcatcggc ggaaccggtc cggtagaaga
    16081 caaagggatg gcagttacgg tccatgatgt agtgcaaaaa caagcctttg ataaaccgcg
    16141 ttaatatttc ttgttcgcgg ccttcttttt tatcggcata gccgagtaaa tagtaatagg
    16201 cttttgtgat gtcgatgtga tgaagatggg tgccaaagcc gcgaatatct ttgatatgtt
    16261 cgcgcttttt aaggctgtag ccatagaaga aaaaaacatc cggtccctga gcgcccaaat
    16321 ataaaacatc gttatcgaca ttttcttcgg cgcgcatctt ggcaaacgcg aagtgcgtca
    16381 tgatcgcggg cataacaagt ctcctttgtt tttgagttca tctactattt aagcacaaaa
    16441 actgataatt cgtcagtctc ttttataaat aaaaaagacg ggacacgccc gtctaattga
    16501 atcgcctact tatgttactc ttgaggatcg tcggcttgcg cctcttcgcc ggcggaaacc
    16561 gcaggagtta tttgctcctt tggagccgcg acttgtttaa ccacggctgc cgcaccgcca
    16621 tgttccttgt tatgaatctc gttgacgtcg ttccaggtgt gcaagaacaa gtactgggcg
    16681 acgagcggtc cgatgccgat catcgatccg acgatgagcc agaggaggta catgcctccc
    16741 ccattaagac gcggagtctg attccgttta cgaatgaaag cagcccatct ttcgacgatg
    16801 ttatacgacc aaataaaagc gtagatgcca agcgtgagaa tcgaaaagag aatgtaaagg
    16861 agcaagcctt tggtattttt gccgtcttct ttgcaagctt catcggtttc cttggcggcg
    16921 gcatgaataa tatacatgcc ttagatgccg agcgtcaaaa aaactaaaaa tcagatattt
    16981 gagcaagccg cgattagtct ttagttccat attccctcct atggttttga tacttacgtt
    17041 aaaacacaaa aaaacatgac ataaatatct tttttgtaac aaatatggcc ttaacggctg
    17101 attattttaa gatagctgcg gcgttttttg tgctgttctt tcggaaagaa cttccactgc
    17161 gcctggacct tcgtgttgtt ttctaagttg agattggtct cgcaatacga gatgttctcg
    17221 cgcatcatgc cgatgattaa ctcgcgcagg atgatggcgt ttatgccaaa cgattgatat
    17281 tcgggcaaga cggcgatgag gcctaagtcg ataacatccg gcttacggag gattttaatc
    17341 attttggtga tggctcccgg agtcaggcga ccgccgcttt tttgaaaagc cggaccgagg
    17401 gaaggcaccg ataagccgaa ggcgatgaca ttttcttttt catcacagac ggtgattata
    17461 aatttgaggc tcagcaaata cataaactgg gtgatgatct gctgcttcat ctctttggtg
    17521 aagggaaccg tgccataaag cactttatag ccttcgtcta aacaatggaa aatgccgtct
    17581 ttatatttat tgatatagtg gcgtttattt cttccggtgc cgaggtggag gcgaaacttc
    17641 tccatcgccg ccgtcgcgac gcggtccaga gtcggatcga gactctccgg cttataaagc
    17701 cgatattcaa gccagtcgat ttctttggcg tagccgcagt tttcaatcag ccgcccgtag
    17761 tattcaaagt tatattgttc ttcaaatgtt gcgatataat cgaaaccttc gatcaaaagg
    17821 ccttctcttt cgaggtcgct gtagccgagg gggccgcata tcgattccat gccattagcg
    17881 agcgcccagc gctccgccgc cgcaaacaag gcgtcggcga cttcttggct gtcgatggcg
    17941 tcaaagcgcg taaaacgggc ccgtttttcg cccatcttct cgttgctctg attttggatg
    18001 ataccagaaa ttcttccgac cactttatcg ccgtcataag ctaagaaaaa agaggacttg
    18061 gatgtcttgt aataggcgtt tttctttgaa aaaaccttca tttcgtcgcc ataaagagga
    18121 gggacaaaat aagggtttcc tttgtataaa tcaagcggga aggatacgaa ttgatggcgc
    18181 attttgcgcg tcgatacttc tttaattgtt atcatgtgct tttttacaac agttaaatat
    18241 tagcaaaata aaatatgaaa agaaaggagg atttattcac cgctgtcgct catcttgatg
    18301 aaggtgataa ttttctcttt tagcgagatt ttgccgacgg tgtaaccata ggctaaaagt
    18361 tctttcttgc agtttaaaaa tgagtggtcg aggcgaaagc cgatgatatc ctgaatttgt
    18421 tcaaaggaaa gaagaatctt tttctgcttt tcgttttgta taaacttcga aagaggatcg
    18481 tatttgctaa tgaaatcaaa gatgactatt ttccgatcag cgacttcaat aatttcgtcg
    18541 ccgcttcctg cggtcccgcc atctcatagc cgggcaccgc caggcgggtg aaaagttcgc
    18601 caatcgcgac cttttcttta tataaagaag tgaagtatga attgagtagc acttcggttt
    18661 gttcttttct ttgaaccggt ccgaacggat tagcaaaata cgagctcacc aagccgactt
    18721 cgctcgtcgt ccctttggct ttgcgctggc cgccacgaaa gatgtcggtc gcttcttcga
    18781 ggttactaaa gaggcggagg ccgatgtcga cctcgctgta gttattagca tagaggagca
    18841 tgtcgatgcg atggtcggcc attataaatt tataagtcgt gacgggaagg acgacgcggg
    18901 cgttgacctg atcgggattt agaaagatgg cccggtcgat cttctgataa gcgtagccgg
    18961 cttcgagatc atcgaggcgg acaaaggcgc cgatttccgt tccgtgagcg tagatatgtc
    19021 cgtctttctt aaagaaggta cccatgtcgt caaagacgat tttcatctgt ttgatatagt
    19081 ctttgccaat gatgcgcagg gcttcaagcg tctcggattt tccggcgccg ctatcgccga
    19141 cgacgacgat gtttttcgtc tcgtcattat ttaagacaat cgagaccatc gccccatgaa
    19201 ttggcagttt tttctggtcg atcatcgcga ggttgtgaag ggttagcagc atctttttta
    19261 gatatccgaa gtagtcgttt ttatctagcc ggctcacaaa acccaaatag atgttatttt
    19321 cctgatcgtg atgataggtg caatcgtatt ccgtctcgct aattccgtag aggtagacga
    19381 tatcgggctt gcggtctttg atttcgtcaa acgaggcgaa ctcaaagaga ttggaaaggc
    19441 cgatgccctc gtggaggaaa tcgcgatgga tgtagacgta ggcgagcaag ggcccgattt
    19501 taatcggaaa ggcaaagaaa tgaagtttat tgatgacgag tttggaaagc gggttttcgc
    19561 cgatttcctg aaataggccc gaacgcgtat tgcttttagt ataggcgata aagggaggcc
    19621 tggtcgcaat cttgctgatg aaagcgatgt tttgcaagtt ttcgtagccc gcatgatagg
    19681 gaacgcggtg atggacatat aaaagattgg cgttgactcc cgcgggaagc tgccggtaga
    19741 cgtggaattg ttggccgaga agattcgttg acacgccgcg atacaaagac aaaatcaggc
    19801 tgttaaaatg atcgattgtc gtgataacgt cgggcgcttt cgcgctttgc gtgtataaat
    19861 gcgaggaagc catcagtcca aagcgctgaa ggcttcgcca gtagtcgtaa aacatctcgg
    19921 taaaatggta gaattcatcg cgttttttta aaagataatg aagggacgga ttatttttgt
    19981 atatatcatt aaaagggaag acataaagga gtttataggc atcgagaaag tgttcgagct
    20041 tgacggtttt aagactctcc atcgtcacgt ctttatcgcg gcgaagttta tcgacaaatt
    20101 gggagaggac gtgtgcaaaa ctcttcgatt gcaaaacgct gacttccgag tcgcaaagcg
    20161 aagcgtcaaa attgatcaag gcataagtat cagtgagatg gaattttttc atcgcgctcc
    20221 tcctagtaac gaacgggcac ttctatctta agcgatagca aggcatcttt taacacttta
    20281 aaaaaattat cgatgtcgcc gacatcaatc gccccgagcg aaccgaggcg aaaggccaaa
    20341 actccgccga cggggcgcgg aaacaaaata aagccgcgct cataacaaaa atcatgaact
    20401 tttttgaaat tccaattaac atcattgggg taaaggaccg agacgacgag gggtccttga
    20461 tgctcgcgtt taatgtattc tttaaacccg aggcgcttga gcccttcgtg aatggcttga
    20521 ttgacgcgga caatccgttt gtatttatcg ttttcgcctt ccgcgaaata ttctttgacg
    20581 ccttgcgccg cggcgtacac taactggacc gggggtgtaa actgcatttc gccatcaact
    20641 tcaaaatggt gatactggcg gtacagatgg cagtaatagg agcgcttggg ataatccttt
    20701 gaaagttcga ttaaatctcg tcttccgata acaaaagaaa ggcccggcat cgccgcgatg
    20761 cccttttgag ccgaggcgag gagaaagtcg acattgtctt tgacaatatc caggggcact
    20821 aaggcgaagg acgatgtcgc atcgacgacg aagacagcct ggtgttcatg aacaatcttc
    20881 ccgatgcttc gaatgtcgtt taaaatgccc gaacccgttt cgtgatgagt cgtgtagacg
    20941 agggtgatat ccggattatc attcaagact tctttaattt tgcccggttc gattggagcc
    21001 gtaaagtcca gtttcagttc gatgtaggac agaccgtagt atttgcagat gtccaccgct
    21061 cttgatgagt aggcgccgtt attaacaatt aatacttttt taccatctgg aagaagagaa
    21121 ttaagcgcga catccatgtt gattgttccg gagccggaaa ataaaacggc gacatggtct
    21181 ttgtcgtttc cgtgagcaat cttaactaag tcttttctca gcttttcaat cacgtcgaca
    21241 aaatcttgtt cgcggtggca catatcggga gccattaact gagctttgac agtgtcggtc
    21301 gtagtcgccg ggccgggatt aagcaatata tttcttttga tattcataag tgcctccttt
    21361 tatattataa aaacttactg gtaatttgat tttcccatta taccaatttt atgctgataa
    21421 agtttagtga atttgcttgt tagaagactt atatagtatt caagtatcat ttgaagtaag
    21481 gacaccttag tcttaatgca cgccttgttt aagtggttga aatcacagcg gacatatcat
    21541 atgtccattt cgacgccata tttgcatttt gcaaaaagtg ctgcgatgta aattgataaa
    21601 aaggcattcc gcttgtagct ttgcggactt cctatttgac tcatttgcgc gatataaaat
    21661 acgtttgcct tctctttttt taagtagttt tgctcgtgaa aggattgtta ggtgaataga
    21721 taatgttgcc tgtcggcatt gaacgcgctc aaaaaaacga cacgcgcata tttcgccttt
    21781 ttatataata agcaaacaat tataagccct cacatattga taattatcaa taaatgagtt
    21841 gtataaatgc tgaaaaacaa ttgggtttaa aaatagagca gaggactcgt ttttcgtgtg
    21901 tcgccattcg taaaattttg taaaagaaaa aaatgccgat tttatcgaca ctttttacaa
    21961 aattttggga tggtggccca ggccgggatt gaaccggcga cacacggatt ttcagtccgt
    22021 tgctctacca actgagctac cgggccacgt acatggcgga ccagacggga atcgaacccg
    22081 cgatctcctc cgtgacaggg aggcatgtta accgctacac cactggtcca aatacgtgct
    22141 tattaattat cttaaataat cctataaaaa tcaagagaca aataaacgga gaagaaaaaa
    22201 gatccctcac aagtgaggga ccctttgact taatctttaa tagttttcgg ccttgagttc
    22261 gaagtgcgct ttgggatggg cgcagaccgg gcagatttcc ggagccttct taccgatatg
    22321 gatgtgaccg caattacggc atttccaaac ggtgacttca tcgcattcga aaattttctc
    22381 gtcggttact ttctttaaca ggtgacgata gcgcttctcg tgttcttttt cgacattggc
    22441 gacaccgagc atcatgcggg cgatttccgg aaagccttct tccttggcga ctttagccat
    22501 ctcggcatac atcgtcgtcc actcatagtt ttcgccagtg gcggcatcaa gcaggttttc
    22561 agctgtcgtc ggaaccgatc cgccgtgcag ggctttaaac cacaacttgg cgtgttcctt
    22621 ttcattatcg gatgtctctt caaagatggc tgcgatttgt tcgtagcctt cttttttagc
    22681 ttgtttggcg tagtagccat acttgacgcg agcttgcgac tcacccgaaa aagcaatctt
    22741 tagattttct tcggttctgg taccttttag ttctttcatt atcgattatt tcctccattg
    22801 ttaattatct aaaaatagtt caccttagtc aaagataatc tttgcgattg ttcagttttc
    22861 tgacttttcg tcatcgcttt tgacatagcg aaaatattcg acattatctg gttttgaagc
    22921 atgttcaacc ataaagttat aggtctgatc gtgaagatcc tgcattgaag tttttaaatc
    22981 ggtatctcgc tttggaaaaa tcggctcacc gacagtgaca accatgcgcg gttttaaaca
    23041 gcgtttgaat atttttcttt tttcataagt ggcgacaatc gggacgacag gcgtattaag
    23101 cttagccgga tagcggaacg aggacgatga aaaggggcgg ataaatgtcg caaacggcca
    23161 aatatgggct tcaggaaaga ccacgatcgc atgctttttg ccaatgtggt attgaatcgc
    23221 ttccaaatat tttttggtgg tgccatttgt ggagggaaga ggcagacaac cgagcatcat
    23281 aacaagattg cgaacaccgg gaatcgaaac ggcgtcaggg tcggcgatga taaaggtcct
    23341 cttcggtctt gtgatgcaaa cctggctgat aaaagcatcc atgatgtggg tgtgattgcc
    23401 atagataaaa tagcctgttt tacggaggtt tttgatggct ttgcggttga cgactttgac
    23461 attcagcgca atcttgcatg tcaaaaagag gacaggaatg gcgacgagaa aatagacgag
    23521 gaacgaaaga attcgccaca atatgttttt atgaatatag cgaaaattct catcgagggg
    23581 ctgagttttg atctttgtgc ccgcaaaatc gtcattgagc agatcgcgat aatagatgat
    23641 gcgcttagga ttttctcgtt ttcttttcat cgtttgcttt gattatatca aaaagcatcg
    23701 actccatctg ttccatggaa tagtcgaaat cgaaccgctt tgcatattcg atatactgtg
    23761 ttcggcaccg ctctttttcg tcgggtgaat caagccagta gtcgattttc tcagctaaat
    23821 ctttggcgtc gttgcattta aacaaattct tttcgcttaa agcgaagtgc cgggttgcgc
    23881 tgcgcctgga atcggcgatg atgggcacca atccgcagga catggcttcg aggcaggaaa
    23941 tagcttcaat ttcgatttcg gcgggatgaa catataaatc agcatagttg ataatatcga
    24001 ttaattcttc acggggatga aagccgagaa tcggcgcgtt ggccaaatct gcgctgcgct
    24061 tgatgatgtt atctcgctgc ggaccgtcgc cggcgaaaac gagttgaatt ctatctttgt
    24121 atttagaaag tttgactgca tcaattaaaa ccttgtgaga tttttcgcgg ctgtagcggc
    24181 cggtaaacaa aatgacgaac ttgccatcca aattctttgg tttgggggca agacgtggct
    24241 taaaggcctt gtgaacgccg ttggagatga catagccgtt tgtttctcca gcatgatgtt
    24301 caaatacctc gcgtataaac tgggtcggat agtgaatcgc atcgacatag cgatagagga
    24361 cgcgatagaa aaatttatag gtcaacagat tggcgaccgg catatccatc ataaaaatgt
    24421 ggttggtgaa gttttcggcc tggacatgaa aaccggccgt gaccggcttt cctaaacgct
    24481 tggcgattcg cgcagctttc gtgcccaaga aaaatggcat catgacatgg acgatgtcgg
    24541 cattcttaat cgcatcagta attatttttg aatcggcttt tgctaatgtg atattgttct
    24601 tcttaacata attgttaagg aaaccgagat ttaatggtgg gacgacatag tagccgtaaa
    24661 gacctgtttt attttcatca gggcagacga cgctgacttc atgccctttg ttttttaaac
    24721 tattaataaa atttactgct gtaatcgtag tcccgttatt ctctatgccg aggacatcgg
    24781 caacgattgt aacgcgcatg ttgatacctc caaaatcgct tggcgagtga tggttgggga
    24841 tagatgataa tcaaagtagt tgcgcgcgtt cccccaaact gcaacgccga ctataaaaag
    24901 tatatactat ttacatatta ttgtctttaa gttctggctc cggctcgttg aatttgactt
    24961 cagccaaggc gagttgtccg gcattcatgc attgaatgcg catctcgcgg ccctgaaaga
    25021 aaaactttgc tgtcgcctcc cataaaaaga cccaggaaaa aatttgcagc atttcaaaaa
    25081 tcagcggaat atccggaaac tgggcagcat tcatcgccaa ttttgcgatt gtcgagatga
    25141 cgagggcgat catgccgatg gcgaataaaa cccaggccac cgctcgattg attttcagtt
    25201 cgcggcgctt atcttcatag ataagccgat aatgatcgag gaacgatttc ttaatccgcg
    25261 actttcttaa atcggagaca tcgccgacat caaaattgac gatgaggggg atttcgacag
    25321 gcgtatagta ggtcttctca tcgatatatt caaaaatatc cggtttcata tcttctttat
    25381 atgaataggg ggaatacagt tcgtcgacat ttgtgagatc gacgctgatg tgagctttgc
    25441 cgtcgggata agttagcgat tcaacgtatt ttttcgtcgc gcttcttgaa tcgcgataga
    25501 gagcttcaat ccgttcagaa aagcgtttat gccgttcttt aattactttt ttatccattt
    25561 tccgctcctc cttttatttt tttcttgagt ttagcggctc gcttgcgctc gatgaggtgg
    25621 tgtaaggctt cgagaaaaga tgagtcgtcg gcgacgatgt cgtcttcgtc ataggcaaag
    25681 acgagatatg ccggtttatt attacgaagg agcaaaacgt atccttcttc gtcgaccttc
    25741 caagccacgc gggagaaatt ttgattagcc tcttttaggc tgacaagttg cttgattctg
    25801 agtttcatgt tttgacccaa agagacttta tcataaaagt aggtagaaaa caacctacaa
    25861 gtcttatcag agtggta
//
LOCUS       DAOSSL010000013        24329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2249_length_24329_cov_15.326522, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000013 DAOSSL010000000
VERSION     DAOSSL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 24329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24329
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2249_length_24329_cov_15.326522"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PLH17_02325"
     CDS             <1..888
                     /locus_tag="PLH17_02325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOR20650.1"
                     /translation="DQMLDMGFLPDIKRIIAKLPTERQTMLFSATMPKEILSLAGSLL
                     NHPVRISIGSVQRPLDAISQNLYRVSKENKAELLIHLLKDKAISSALIFCRTKNGANR
                     LTKKLLNQSYTAEVIHGNKSQNARTSALKNFKNHESRILIATDIAARGLDISQISHVI
                     NYDIPQTPETYLHRMGRTGRAGEVGETISFCSRDELSLLKDIQKHIGMDIPVLKSPIV
                     FSVVETADSKSTSTQANNSPKAQANNSQRHDSGRSADAGHFRHSQSKQSGPRGHQQEQ
                     KASDAVNQKSAFTNKRKYK"
     gene            1116..2588
                     /locus_tag="PLH17_02330"
     CDS             1116..2588
                     /locus_tag="PLH17_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20651.1"
                     /translation="MISAFILFGSFFFGLLGLLFYIPIVGLFIPGASINTIDMTPMFV
                     FPVLIVIFAFSVHFITDEAMDRIGRGGEKLIKVVPYLVLASLVVYVAYIIRGNDFMLF
                     TPYYWFHNYLDTFDLIFIIIQFLVAFLIGLSIFGATGFSYDRYVDVTYESTAYSGDFE
                     VKRSDSYVSRSQFYLTAFIGVLIGVLMSFTAFAIVASIFYFSFVIIGRFKVKALKDKP
                     HNFRARDRFVGKQIVTSVSLALVAAAAVTLGVLVNDYRLEKPTDMMNIVLNDENDDDF
                     LSASLHSNTTYIHEEVRYNLNLGVRLFSKKSAYDIDGLQIRIDLTCQKIEVETGEVQA
                     TLTIGETLDFDHVYSDYIFREIAPYTNVSPHHLAYVVLTQEINLVKGTAACRYDISKG
                     FPYRKIGAKDLDGYFLLGVGYPEEDGYHYFVQILTNLYIKELFVELNVGNNQIIELNF
                     ADTFLLENQRVEVHVSENYQNTDAFSYDYAKGYIAFFYVN"
     gene            2829..>5009
                     /locus_tag="PLH17_02335"
     CDS             2829..>5009
                     /locus_tag="PLH17_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOR20652.1"
                     /translation="MYDNLLAKKNRKFLLFSTACLLLGVAVFVIGHNSQLLQHLTYGD
                     SSYTITLNEANSPLNLAMEEYGSGEGYGRYVQFKYTTAKLQTGSHAVLGEDGTLYNSD
                     TTRITSIKSITATFTGGQATLEYGPTKTTIGDPQVLTTATPYYFSTDPYFFVLTNTGT
                     GSLVLASLVINYSCQDVHSTIEFVTNGGSSVAPITQAHGSVVEAPSDPTKTSYLLDGW
                     YTEPALINEFSFTTMPIDDVVLHAKWIIDPAYPVLTLSEFKALTAPENAEHHFVRGVV
                     LLAKSEMEIIILADATDTLIVFGYQEALVGDEVRVGGFYAAEETLVTLEPAPGNEVSV
                     DLYSHNNEIALTPTAMSVAAFNTLDPDSPASWVVYAEINGTIGADDNTHQISLSDGED
                     TMPVVVIGEGDYSYIRDYAGFRVNIRGVILPNMDNPEEVFLMFIFNGDEDFIDLDYYG
                     EGEDQELLTLLEGMFRGAFESPTYFPGQFVDLPADHPVVPVALTYEPYGAYASEYSTV
                     TNRISDDVSAEIYIDIHVNATLTLNEVVDKAFDIQLHVGPDLIITIAELKLLPDSNEN
                     PLVIQCVVLNSQASGENFMLLVADGTGIIYINTDNGSIAPGDEVVAVGYKMTMETIVV
                     LFNDPHRMVDHTVATGQSMPMTPTAITIADFIALEHAYVAADFHYYELSGTLAYLNPG
                     NPSESLFVLTSGSDSLYVYPTSPASRATLSTYAGENVTIRGIAML"
     assembly_gap    5010..5109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5110..6924
                     /gene="typA"
                     /locus_tag="PLH17_02340"
     CDS             <5110..6924
                     /gene="typA"
                     /locus_tag="PLH17_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOR20653.1"
                     /translation="IRNIAIIAHVDHGKTTMVDQLFRTAHLFRDNETSNERLMDSNDL
                     ERERGITILAKATGIQYKGLQINILDTPGHADFGGEVERIMNMVDGCLLLIDAHESTM
                     PQTRFVLKKALEARVKPIVVINKIDRPNIDIKKVLNDVLELFIALDAPEEYLDFKVAY
                     ASALLGTSSLSPDPKDQKPGMEAILDLIISEISGPKVDEDGPLQFQPSLLDYNDFVGR
                     IGIGLVKSGTMRVGENIVLSRLDKTNKLGKIMKMYRFVGINKIDLEEARAGDIVGVAG
                     LTDINVGETICNPDHILPLPPITISEPTLQMTFAPNSSPFAGRDGKFVTFRQLQARLE
                     KEIQRDVALKVSLIDGSDRFLVSGRGELHLGILIENMRREGYELEVSKPKAIIKIIDG
                     QAHEPFEELFLDVPSESLGAVMDVLANRQAEVVNIEYGESNTRLIYNIAAAALIGLHA
                     QIMTLTKGYGLMSHLYKNHRPVTNFQVKGRTLGVLIASDTGLSTTYAIEGLEARGTMF
                     ILPQTEVYEGMIVGENRYSSDLIINVTRAKELTNMRMSTKEQKVVLKAPRLMSLENCL
                     AYLNDDELLEITPSTYRMRKMYLSELERKRKTPRKPVE"
     gene            7047..7883
                     /locus_tag="PLH17_02345"
     CDS             7047..7883
                     /locus_tag="PLH17_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOR20654.1"
                     /translation="MKKKIIDQDYILSNGVAIPKIGFGTWRVKPGKEAYESVLMALRN
                     GYRHIDTATVYKNEKSVGQAIKDSALKREDIFVTTKLPSHVKSCDGAKKEFAKSLKRL
                     GLSYVDLYLIHAPWPWIAIGTDHTQGNIEAWKAMIELYNEKKIRAIGVSNFAPSDIEP
                     LIQATGFVPHVNQLPFYVGKPQLENRAYCQKNNILFEAYSPLMSGRVFKLRLVNQLAE
                     KYEVTPAQIALRYTIQQNTLPLPKSTHEGRIINNAELDFVIAPEDMEKLEALHLHPKS
                     RF"
     gene            7905..8381
                     /locus_tag="PLH17_02350"
     CDS             7905..8381
                     /locus_tag="PLH17_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOR20655.1"
                     /translation="MENTNLKIAVIIHSKTGNTKSVAERIKEKLVARGHQVRLEEIVA
                     GNDEEMDLAKIILTKNPESKGYDGYVFGAPVRAFSLSPIMKAYLSKTQDLEGKPVYCF
                     LTQHFRFRFLGGDNALKQFELALNDKSARILGTNIVNWKSKKRARLIEETVDLVKF"
     gene            complement(8451..10238)
                     /locus_tag="PLH17_02355"
     CDS             complement(8451..10238)
                     /locus_tag="PLH17_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20656.1"
                     /translation="MRDFVENHVAHQDVKLLVRLLGYFKPYTGKFIGAFLLMIVTTFT
                     NMVMPLASGLAIDLMVDPAYSLEQKVTVVFIGAAILLIITGLSILIGYFQSIMLQRIG
                     QKITDQLRTEVFNHIESLSIGQINQLPVGKLVTRATNDPGNISEMFTNTIVNLIRNFL
                     FMLVIAIIMFIIEWRMALITMAVIPLIFVASVLFRRFSRSAYRSVRNNVSEVNAFLSE
                     NLSGMKITQVFNQEDKKIKEFTTINQKLQKSYFREIYTFGIYRPVIYFLAMAATLMTI
                     YFGVSSIFEGFLTIGLFVSFYVYVGQFFEPIQQIAEQFNSLQNGFASAEKIFDVLDTK
                     PAINDAKDAIELANFRGSIEFRDVWFSYIPDEWVLKGISFKVEPNETIALVGATGSGK
                     TTILQLIVRNYDIQKGQILIDGIDIRRIKRSSLRSFVGQMLQDVFLFSGTIRENITLK
                     NDAFSNDDINKAADYVGLDHVLKKLPEGLDHIVRERGNNFSSGERQLISFARAVTYKP
                     TVMTLDEATANIDSETEAVIQASLAKMMNISTMIIVAHRLSTIQHCNKILVMQKGEIV
                     ESGNHQQLLKKKGLYFNLYQLQYEHQEAL"
     gene            complement(10231..11991)
                     /locus_tag="PLH17_02360"
     CDS             complement(10231..11991)
                     /locus_tag="PLH17_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20657.1"
                     /translation="MIFLKHFRHYYLKYGLAFLFGVAILVVIDWYQLEIPRIVKIIID
                     GVDTGAISHLSQVLEPLLTVALIVGGITIGRFLWRLLLFGTGRKIETDIRQEMFTHAT
                     KLDQNFYSQQKIGGLMSYFINDLGTVRELFGFGLLMLVDGVVLGAFVLIRMFTLDWVM
                     TLYSFIPIALMGVLVFFLELKMEQKFKLRQEAFEQASDFAQESFTGITVIKAYVREAA
                     EALGFKNKSQEVYRKSMDHMRYAIIVNIIIDSMISLVILAIITFGSIMIAKGNLSSGE
                     LTEYISYFFTLLWPVFAISWFMNINGQAQASAKRIYAFLESKAIVNENDKAIKDAVLD
                     GSITFNHLTFAYPDAEEPILKDVSFDIKAGEMVGILGKTGSGKSTLVELLLHVYNVEE
                     EMIKFGPYDHAKLSLKTLRGHIGYVPQDNFLFSDTIKNNIGFAYEQIGEDKLLEVGKL
                     ADIHDDVMGFKEQYETILGERGVTISGGQKQRVSIARALAKDPTILILDDSVSAVDTK
                     TEEDIIRNLRRVRKGKTTLMIAHRISTVRRLDKIVLIDAGRLAGFGTHADLLKTNSLY
                     KEMYNMQKLEELIGEEKTHA"
     gene            12156..13220
                     /locus_tag="PLH17_02365"
     CDS             12156..13220
                     /locus_tag="PLH17_02365"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOR20658.1"
                     /translation="MAKDKHLSHNFVKNLLAEDSSRYPYTPAKLAYFCDLISEGYKSY
                     RISCSQSAGVKPFEEFAKEIIQAHNSKFNSMTEDFKQNYFRQADYLRKIVSQKCQSLI
                     NKNDPDHIFTYVNPKDDSRVDHLKEIINGYYSLLKDAGDGSLAQAMVKRIINQMISGI
                     RLISVKQYADAFIIWRSLLENASYLKIIIKGGKKTSNLFAERKNTMKKVMRLAPATKA
                     EMDSLRDQAHNRAKSAKWWEHQRFVWAKGVVNIKGELSSRTLQEAAGLDKYYIHYQVA
                     SVFTHEHLINENDFQNISLFDYVIELYWRTFEEIRLDIIGLFKLKDEQLEYINKHEKN
                     LRLALKSTHPKFDEFALMIS"
     gene            complement(13222..14277)
                     /locus_tag="PLH17_02370"
     CDS             complement(13222..14277)
                     /locus_tag="PLH17_02370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR20659.1"
                     /translation="MRFSKYVDEKTKREYFQHSHLIYLSTFFAFFAIIPLFFLSRSIL
                     ITAISFVTMGITAIAVLINRRGYYGLASFIFILIITIQAAAEVIVFGLEIGFQYYFFN
                     MVVLIVFAKWNNIQKLIAASIEALVFIGVLLVALRFSPLIVLSNPLALVFHIINIVLN
                     ILGVARTAYHFLSIARGAEKDLLTYAQTDFLTSLPNRAAYHRFVYEMKERFKSKPFPP
                     VVIMMIDIDNFKTINDKYGHAAGDEVLIILGKTFKERLQGGDFLARYGGEEFVLIHLA
                     QNEEEAKQFAESLRQEIKGRTFHFEEKDIRLSISVGALYRSPTNKKDCEEALEEADIL
                     LYQAKKEGRNRVAFKSI"
     gene            14656..15633
                     /gene="asnA"
                     /locus_tag="PLH17_02375"
     CDS             14656..15633
                     /gene="asnA"
                     /locus_tag="PLH17_02375"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOR20660.1"
                     /translation="MYQSKLNLLETEKAIKFIKDEFERRLATKLNLIRISSPVVVSKE
                     SGINDYLNGYEKPVAFHYQGEELEIIQSLAKWKRMALKRYGFKIGQGLYADMNAIRKD
                     EIIDSIHSIYVDQWDWEKIITRKQRTKEYLFKTVKVIYSVIQKMERIVNRRYPTLFFK
                     LPDEITFIDSQELEDRFPQLTSREREYQVTKECKAVFVTRVGGLLNSGVIHDGRSPDY
                     DDWDLNGDLLLYDAVNDAVLEVSSMGIRVDEVSLVAQLKERNALDRLQYPYHQALLNG
                     QLPLTIGGGIGQSRMCQFLLEKKHIGEVQASYWPPEMLAALEQENIIVL"
     gene            15708..15947
                     /locus_tag="PLH17_02380"
     CDS             15708..15947
                     /locus_tag="PLH17_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOR20661.1"
                     /translation="MEKLISVEQSVFALSQSYPEIVEILASLGFSDITKPGMLASVGR
                     FVTLKQGAALRNIDLQIVKDALIAHGFSFKEKKNE"
     gene            15940..17160
                     /locus_tag="PLH17_02385"
     CDS             15940..17160
                     /locus_tag="PLH17_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOR20662.1"
                     /translation="MSEFINNQSIRQEKLKKLIRSLHDGKPLEQAKQEFKEEFGSITT
                     AEITAMEQGLIKEGMAASEIQRLCDVHAAVFEGSIADIHAPKDEVDDIPGHPVHVFLE
                     ENERLEKLLKEEIEPYLNQSGKTAILMLRVGFDRLKEIDKHYARKEYLFFPHLERKGI
                     TAPPKVMWGVDDEIRADIKSVIASLSAVAIDEAATFALIRTTLTKIRDMISKENNILI
                     PLLKENLNLFNWISVELATPDTGWFLEKPKVKWEEKGSSIEKPKKKKEIVSKTDTEIR
                     FDAGSLSAEQINAMLNALPFDMTFVDHKGYVRYFTQGKERIFARPATIIGRHVSMCHP
                     PASVHVVEGIIESFRSGRKDHEDFWIRMKDMFVYIRYYAVRNARGEYLGTLEITQNIK
                     PITELQGEKRLVDK"
     gene            17300..19243
                     /locus_tag="PLH17_02390"
     CDS             17300..19243
                     /locus_tag="PLH17_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOR20663.1"
                     /translation="MTNQISKTTFLKYLRCPRAAAFEGDAAAIVRDYQRNLAHIGGEE
                     KAKILEEETKEKLRQLFIRLIENDEGGAESEAEIESGDFDGLLKDDLTLKAMLSTYFK
                     IEQLTAEKAKKTFGGQVLSGMRVGDQVIGQRYINLFKDGFNFHSFVDVVQEDDSAVRI
                     IEAKSTTSKKFAKLGPKINKELCPIFERSPFGVLRLKEDFEGFKPFDKYYDNREKLMD
                     RFRDVGRYVYDLAWQRYIIENSTDDKREYRYYLAVLNSDYVFDGRQDANGENIYNADD
                     LIVLVDLTQVTKDMLLIVDRDVNTVIDRINKPDFSRVPLEKPKCKINGSYDRCPWLKI
                     CMKDLMVPDKNSLYVYRGGHTGFGPASENSKEKAQKEKIEELIWRGITKALDIDYDWL
                     NETQKVQYEVIKSGQPFIDKKVIKAMLKDLKYPLFHLDFESMNFPIPMYRGEVPYQQS
                     LFQYSLHIERTPGVCDKEKDNVSFLSTGKTDDREEFIKSMIKNIPANQGGTVIVYNAI
                     FESGRLRELIEMFPKYAVELNSILERIYDLKDILDPSKKIIADYEYLKSKPCAFNYYN
                     ESFQRSYSIKKVLPVFAPHLDYANLDEVHNGLDAQAAFMRLKYLEGKAFDETYKNMIE
                     YCKQDTWAMFEIMQGLRKLINEK"
     gene            19233..>20458
                     /locus_tag="PLH17_02395"
     CDS             19233..>20458
                     /locus_tag="PLH17_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOR20664.1"
                     /translation="MKNKRLLLIAAVLILSSCVNGGGTGSESSQDTSSGTSGDTSSNT
                     SSDTASDTVSSVTGDTSSSSETGRPTSGTEIIDFYAINDFHGAISKNDYFGEPGLARV
                     AAYLKDKKDDAPENSVIISSGDMWQGSYDSYHNRGRVITEAMDDIGFDSMTIGNHEFD
                     WGKEFIEDNEGWADFPLLGANIMEYPDTSVKSSVGQEYAIIERGFLKIGVIGVIGQKQ
                     ITSINSRYMQDLYFEEPTQIIKNLSAELREEHDVDIVVLSIHAGQGEVDYSISQGKYV
                     DAVFCSHTHQVEQQVVNGVPFIQGGSNGKHVSNIRLSFNYATGQVNHVSSQNTYEGQL
                     NSLTPDTGVQAIISQFAQSSDSDKNQVVGTLTNNLNRYDTLPNMANYTAAMKAVENGY
                     DIDFAMTNVGRSNIPGG"
     assembly_gap    20459..20558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20787..23483
                     /locus_tag="PLH17_02400"
     CDS             20787..23483
                     /locus_tag="PLH17_02400"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOR20665.1"
                     /translation="MYETKKIDELLVEHAVDPAVGLTSEEACRRLEKNGKNKLVEKKR
                     RSIFLTFLLQINDPMIYVLLAAALISALVTIFGEGHDDWADVIIILAVVLLNATIGTI
                     QEVKAENALEALKKLSSPTATVRRDGKVFEIKAEDLVLGDIVILEEGRSIGADMRLLK
                     EYNLKTDESSLTGESTPIEKDYQVVFSEPVGVGDRINSVYMSTPIVYGHGEGIVVATG
                     EKTEIGKIAKMLADEKEEATPLQKRLADLSKILGIIAIVIIVAIFAVALIQKRDPIEM
                     FITAISLAVAAVPEGLPAVVTIVLALGVQRMVKVNTIVRRLPSVETLGAVSVVCSDKT
                     GTLTQNKMTVVRAFVDHEIIDSEHFKGDSVSFLARGMSLCSNASIDSGVYGDPTEIAL
                     VEFGNKLGLRKKDLEGVHPRINELPFDSVRKMMSTHHEHESGAIVFTKGALDSVIKHT
                     THIFINGQVRPITEKDFKQINEGSAYMARNALRVLALAYKESSELSEDNLTFVGLVGM
                     VDPPRPEAGKAVAIFKKAGIVTVMITGDHRDTALAIARELKIAHEESEVLSGDELSKM
                     SFEELQQAVKTVRVFARVSPENKVSIVKAIKANGYIAAMTGDGVNDAPSLKAADIGIA
                     MGITGTDVAKGAADMVLTDDNFASIEKAVEEGRGIYANIKKTVYFLLSSNIGEVISMF
                     VGIVLGLPVPLIAIHILWVNLITDSLPAIALGSDEKADNIMEDKPRDEKESLFARGGY
                     FFIITYGIVIAIITLFGFLLFPTIHIVESGLPFTLANLIAVTNEPTMLRLSQSTAFSV
                     LGVSQLFHMIGMTNTEKSFVHVFKSKNWLLLIAFVFGLGMQILITEIPFLEDFFSTAQ
                     LTLVEWLYVIALSVVPLVIHEIIVLIKFIKKQIKKRKLASGN"
     gene            23541..23617
                     /locus_tag="PLH17_02405"
     tRNA            23541..23617
                     /locus_tag="PLH17_02405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23575..23577,aa:Thr,seq:cgt)
     gene            23634..23709
                     /locus_tag="PLH17_02410"
     tRNA            23634..23709
                     /locus_tag="PLH17_02410"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23667..23669,aa:Val,seq:gac)
     gene            23791..23864
                     /locus_tag="PLH17_02415"
     tRNA            23791..23864
                     /locus_tag="PLH17_02415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23823..23825,aa:Gly,seq:ccc)
     gene            24148..>24329
                     /locus_tag="PLH17_02420"
     CDS             24148..>24329
                     /locus_tag="PLH17_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOR20666.1"
                     /translation="MEDYRQKCINLLKERGVTLNDIADAARFLQADYHVDLLKEELLD
                     SVLSVLAKREVQLAIMT"
BASE COUNT         6720 a         5573 c         5483 g         6353 t
ORIGIN      
        1 gatcagatgc tcgacatggg ctttttgccg gatattaagc gcatcatcgc caaacttcct
       61 acggaacggc aaacaatgct tttttccgcc accatgccca aagagattct atccctcgcc
      121 ggaagtcttc ttaatcatcc tgtccgcatc tccatcggca gcgtccagcg ccccctcgac
      181 gccatttcgc aaaacttata tcgcgtctcc aaagaaaaca aagccgagtt gctcatccat
      241 ttattaaagg acaaagcgat ttcttcggcg ctgatcttct gccggacgaa aaacggcgcg
      301 aaccgcctaa ctaaaaaact attgaaccag tcttataccg ccgaagtcat ccacggcaac
      361 aaaagtcaaa atgcccgcac ttcggcctta aaaaacttca aaaatcacga aagtcggatt
      421 ctgattgcca ccgatatcgc cgctcgcggt cttgatatca gtcagatttc tcacgtcatt
      481 aattacgaca ttccgcaaac tcccgaaact tatcttcatc gcatgggtcg gaccggacgg
      541 gcgggagaag ttggagagac gatcagtttt tgttctcgcg acgagttatc gcttcttaaa
      601 gatattcaga agcatatcgg aatggatata cctgtcctta agagtcctat tgttttttct
      661 gtcgtcgaaa ccgcggacag caaatcgact tccacacagg caaacaattc tccgaaagct
      721 caagctaata attctcaacg tcatgattca ggtcgttcag ctgatgctgg gcactttcgc
      781 cattcgcaaa gcaaacagag cggcccgcgc ggtcatcagc aagagcaaaa ggcgagtgac
      841 gccgtcaatc aaaaaagcgc ttttaccaat aagagaaaat ataaataaaa gacgaatctt
      901 tttaaattat taagggatgc aaatatttac atatcatgca aaaaaccatg gcgaagcaaa
      961 tatagatata aatacttttc gttcaaatat tgacgcctgt atataaataa ggatttaaga
     1021 aaaagtccga acagaacacg ttttttctaa ctttcataat tagttcttga tagctaattc
     1081 gtcttgtgtt acaaatatag cgttgagaat ttttaatgat tagcgcattt atcttgttcg
     1141 gatcgttttt cttcggcctt ctcggtttgc tcttttacat cccaattgtc ggtcttttta
     1201 ttccgggcgc ctcaatcaac accatcgaca tgacgccgat gtttgttttt ccggtcctca
     1261 tcgtcatctt cgcattttcc gttcatttca tcactgatga agccatggat cggatcggtc
     1321 gcggtggtga aaagctaatt aaggtcgtgc cttatctagt cttggcgagt ctcgttgttt
     1381 atgttgcata catcattcgc gggaatgatt ttatgctttt tactccatac tattggtttc
     1441 ataattatct tgacaccttt gacttgatat ttatcatcat tcagtttctt gtggcctttt
     1501 taatcggctt atccattttt ggtgcgaccg gcttttcata tgatcgatat gtcgatgtca
     1561 cttacgaaag cactgcatat agtggcgatt ttgaagttaa acgttctgat tcttatgtca
     1621 gtcgctctca attttatcta acggctttta tcggcgttct tattggcgta ttgatgagct
     1681 ttacagcctt tgccatagtg gcttctatat tctatttttc ctttgtcatc atcggaagat
     1741 ttaaagtcaa ggccctcaaa gacaaaccgc ataattttag agcccgcgac cgctttgtcg
     1801 gcaagcagat cgtcacgagc gtgtctctag ctcttgtcgc cgccgcggcc gtaactttgg
     1861 gtgttttagt caacgactat cgacttgaaa agccaaccga tatgatgaac attgttctaa
     1921 atgatgaaaa tgatgatgat ttcctgtccg cttctttaca cagcaataca acctatatac
     1981 atgaggaagt ccgttacaac ttaaaccttg gcgttaggct cttttctaag aaaagcgcat
     2041 acgatatcga cggtctgcaa attcgcatcg atctgacatg tcaaaagatt gaagtggaaa
     2101 ccggcgaagt tcaagcgacg ctgacaattg gcgaaaccct ggattttgat catgtatatt
     2161 cagactatat ctttcgcgaa atagcgccct acaccaatgt ctctccccat cacttggcgt
     2221 atgttgtctt aacccaggag attaatcttg tcaagggcac cgccgcatgc cgctatgata
     2281 tttcgaaagg ctttccgtat cgcaaaatcg gcgcaaaaga tttagacggc tattttttgc
     2341 tgggtgtcgg ctaccccgag gaagacggct atcattactt cgtccaaatc ctcaccaatc
     2401 tctacattaa agaattattt gtcgagctga atgttggcaa caatcagatt atcgagctaa
     2461 atttcgccga caccttctta ttagaaaatc agcgcgttga agttcatgtt tcggaaaact
     2521 atcaaaatac tgatgcattt agctatgact acgccaaagg atatatcgcg ttcttttacg
     2581 tgaactgatt cgtctttgta aaagtatttt cttcgaaact aagcatctaa tcaaaacatt
     2641 gagccgtgaa ccctgatccg gggtttttac gattatgaac atatggtatg aaaacttgat
     2701 ttcataacta ttgctttatt ttgtgaatcg tatctttact aataatagag tgttttttgt
     2761 caaatcttag cagccatcaa gacagtagtc tcgcccctta atagcataaa ctaaaaggag
     2821 tcaacattat gtacgataac ttactggcaa aaaagaaccg caagtttctt ttatttagca
     2881 cggcttgtct gcttttaggc gtcgccgttt tcgtcatcgg tcacaacagc caattgcttc
     2941 agcatctcac ttatggtgat tcttcttaca ccatcacgct taatgaagcc aattcgccgc
     3001 tcaatctagc gatggaagaa tatggcagtg gcgaaggcta tggccgctat gttcaattta
     3061 aatacacgac ggcaaaactt caaaccggaa gccacgccgt cctcggcgaa gatggcacgc
     3121 tatataatag cgacacgacg cgcattacat cgattaaatc gataaccgcg acttttacgg
     3181 gcggccaggc gaccctggag tatggtccga ccaaaacgac cattggcgat ccgcaagtat
     3241 tgaccacggc cacgccgtat tatttttcga cggatcctta tttctttgtt ctcaccaata
     3301 ccggaaccgg cagcttagtc ttagcctcac ttgtgattaa ctacagctgc caagatgtcc
     3361 attcgacgat cgaattcgtc accaacggcg gctcctccgt cgcccccatc acgcaggctc
     3421 acggaagcgt tgtggaagct ccgagcgatc ctactaaaac gagctacctc ctcgatggat
     3481 ggtataccga gccggccttg attaacgaat tcagcttcac gacaatgcct atcgatgatg
     3541 tcgttttgca cgccaaatgg attattgatc ccgcctatcc ggttttgact ctttccgaat
     3601 tcaaagcgtt aacagcccct gaaaacgccg aacaccactt tgttcgaggt gttgttttgt
     3661 tggccaaaag cgagatggaa attatcattc ttgccgatgc gaccgatact ctgattgtct
     3721 ttggctatca ggaagctctc gtcggcgacg aagttcgcgt gggaggtttt tatgccgctg
     3781 aggaaacatt agtgacttta gaaccagctc cgggtaacga ggtcagcgtc gatctctatt
     3841 ctcataataa tgagattgcc ctcaccccga cggcaatgag cgtggcagcc tttaacaccc
     3901 ttgatcccga cagccccgca agctgggtcg tctatgccga gattaacggc acgatcgggg
     3961 ccgatgacaa tacccaccaa atcagcctga gcgacggcga agacacgatg ccagtcgtcg
     4021 tcatcggaga aggcgattat tcatatattc gcgattacgc cggattccgc gttaacatcc
     4081 gcggcgtcat ccttcccaac atggacaacc ccgaagaggt cttcttaatg ttcatcttta
     4141 acggagatga ggattttatt gacttagatt attatggcga aggcgaagat caagagttat
     4201 taacactttt agaaggtatg ttccgcggag cttttgaatc gccgacttac tttcccggtc
     4261 aattcgtcga tttgccagcc gaccatcctg tcgtacctgt cgctttaaca tatgagccat
     4321 atggtgcata cgccagcgaa tacagcacgg tcacgaatcg catttccgac gatgtgtccg
     4381 cggaaatata tatcgatatt catgtcaatg cgaccctaac cctaaatgag gttgtcgata
     4441 aagcgtttga catacagctc catgtcggtc ctgatttaat catcactatc gcggagctga
     4501 aactattgcc ggacagcaac gagaatcctc tcgtcattca gtgcgtcgtc ttaaattcgc
     4561 aggcttctgg cgagaacttc atgcttctgg tcgccgatgg caccggcatc atctatatca
     4621 acaccgataa cggcagcatc gctccgggtg acgaagtcgt cgcagttggc tataagatga
     4681 cgatggaaac tatcgtcgtc ctttttaatg atcctcatcg catggtcgat cataccgtcg
     4741 ccaccgggca aagcatgccg atgacgccga cggcgatcac gattgccgac tttatcgcct
     4801 tagaacatgc ttatgttgcc gccgatttcc attattacga attaagcggc acacttgctt
     4861 atcttaatcc cgggaatcct tctgaatcct tatttgtttt gacctcgggt tctgattcgc
     4921 tctacgttta tccgaccagc ccggcctcgc gagcgacgct ttccacctat gcgggtgaga
     4981 acgtgacaat tcgcggcatc gccatgctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnna ttcgcaacat cgcaattatc gctcatgtcg atcacggaaa aacgacgatg
     5161 gtggaccaac tgtttcgcac cgcccatctt tttcgcgaca acgaaacatc aaatgaacgg
     5221 ctgatggatt ctaacgacct cgaaagggaa cgcggcatca ccatcttagc caaagcgacc
     5281 ggcattcaat ataaaggtct ccaaattaat atactggata ctccggggca cgccgatttc
     5341 ggcggtgaag tcgaacgcat catgaatatg gtcgatggtt gtcttcttct tatcgacgcc
     5401 cacgaaagca cgatgccgca gacgcgtttc gttctaaaaa aagctttgga agcacgcgtt
     5461 aaaccgatcg tcgtcatcaa taaaatcgac cgacccaaca tcgatatcaa aaaagtctta
     5521 aacgatgtct tagaactctt catcgccctt gatgctcccg aagagtattt agattttaaa
     5581 gtcgcctatg cttccgcttt attggggacg agcagccttt cgcctgatcc aaaggatcaa
     5641 aagccgggca tggaggcgat tctcgatctc atcatcagcg agatcagcgg accgaaagtc
     5701 gatgaggatg gccctttgca atttcagccg agcctcctcg attacaatga ctttgtcggc
     5761 cgcatcggta tcggccttgt caaatctgga acgatgcgcg tcggcgaaaa catcgtcctt
     5821 tcgcgtttag ataaaaccaa taaactcggc aaaatcatga agatgtatcg ctttgtcgga
     5881 attaataaaa tcgatttaga agaagcccgc gccggcgata tcgtcggtgt cgccggctta
     5941 accgacataa atgtcggcga gacgatttgt aatcctgatc atattttgcc cctccctccg
     6001 attaccatca gcgaaccgac cttgcagatg acgtttgctc ccaacagctc gccttttgcc
     6061 ggccgcgacg gcaaatttgt cacctttcgc cagcttcagg cccgccttga aaaagaaatt
     6121 cagcgcgatg tggccctcaa agtatctttg attgatggca gcgaccgctt tctcgtctcc
     6181 ggccgcggtg aactccacct tggcatcttg attgaaaata tgcgccgtga aggctatgag
     6241 ctcgaagtga gcaaaccgaa ggccatcatt aaaataattg acggtcaagc ccatgagccc
     6301 ttcgaagaat tatttctcga tgtcccttct gaaagcttgg gagcggtgat ggatgtgctg
     6361 gctaaccgcc aggctgaagt tgtcaatatc gaatatggtg aaagcaacac gcgcctgatt
     6421 tataatatcg ccgctgccgc tttaattggt cttcacgccc aaatcatgac cttaactaaa
     6481 ggttatggtt tgatgagcca tctctataag aaccaccggc ccgtcactaa ttttcaggtc
     6541 aaaggccgta ctctcggggt gctgattgcc tctgataccg gcctgagcac gacatacgcc
     6601 atcgaaggcc ttgaagcgcg cgggacgatg ttcatcttgc cacagacgga agtttatgaa
     6661 gggatgatcg tcggcgaaaa tcgctacagc agcgacttaa ttatcaacgt gaccagagcc
     6721 aaagaactga ctaatatgcg catgtcgacc aaagagcaaa aagtcgtctt gaaagcgccg
     6781 cgcttaatgt cgcttgaaaa ctgccttgcc tatttaaatg acgatgaatt attagagatt
     6841 actccctcga cataccgcat gcggaagatg tatctcagcg aactggaaag aaagcggaaa
     6901 acgccgcgaa aaccagtcga atagttgctt ctttcaatta taatttaatt cctttattaa
     6961 tgtcttttca tgtccataga gtagaataat aagtgatgtc atgttctttt tatccgtgga
     7021 tactaagcag gcagtaaagg agaaatatga aaaagaaaat catcgaccag gactatatct
     7081 tatctaatgg tgtcgccatt ccaaaaatcg gctttggcac ctggcgcgtc aagccaggca
     7141 aagaagctta tgaatccgtc ctgatggctc ttcgtaacgg ctaccgccac atcgataccg
     7201 ccaccgtcta taaaaatgaa aagagcgttg gccaggccat caaagattcc gcccttaaac
     7261 gcgaagatat cttcgtgacc accaaacttc cctcgcatgt caaaagctgc gatggtgcaa
     7321 aaaaggaatt tgcaaagtct ttaaaaagac ttggtcttag ttatgtggat ttatacttaa
     7381 ttcacgcccc gtggccgtgg attgccatcg gcaccgatca tactcaaggt aacatcgagg
     7441 cttggaaggc gatgatcgaa ttatataacg agaagaaaat tcgcgcgatt ggagtatcca
     7501 attttgctcc gagtgatatc gaacctttaa ttcaggcgac cggttttgtc cctcatgtta
     7561 atcagctgcc gttttatgtc ggaaaacccc agctcgagaa tcgcgcttac tgccagaaga
     7621 ataatatttt attcgaggcg tattcgccgc tgatgtccgg ccgcgttttt aagcttcgcc
     7681 tcgtcaatca actcgcggaa aaatatgagg ttacgccggc gcaaatcgcc cttcgctata
     7741 cgattcagca aaataccctg ccgcttccca agtcgacgca cgaagggcgc atcatcaaca
     7801 atgccgaact cgacttcgtc atcgcacctg aagatatgga gaaactagaa gcgcttcatc
     7861 ttcatccaaa atcacgcttt tagcatttat agcgggggta atatatggaa aacactaatc
     7921 ttaagattgc cgtcatcatc cattcgaaga ccggcaacac caaatcggtg gccgaacgaa
     7981 ttaaagaaaa actcgtcgcc cggggccatc aagtacgact tgaagaaatt gttgctggta
     8041 atgatgagga gatggatctt gctaaaatta tcttaacgaa aaatccggag agcaaaggct
     8101 atgatggcta tgtgttcggg gctccggtcc gggcttttag cctttcgccg atcatgaagg
     8161 cgtatttatc aaaaacgcaa gatctcgaag gcaaaccggt ctactgcttt ttgacgcagc
     8221 attttcgctt tcgcttcctg ggaggcgaca atgctttaaa gcagtttgaa ctggctctca
     8281 atgataaaag tgcccggatt ctaggcacca atatcgtcaa ttggaaaagc aagaaacgcg
     8341 cgcgattaat cgaagaaacc gtcgatttag ttaaattcta gtaggctgta attcaacaat
     8401 cggaaaattg cttcttgcct gagcgagtta ttaggcgtca gaaatagcag ttaaagagct
     8461 tcttgatgct cgtactggag ctgatagaga ttaaaatata aacccttttt ctttaatagt
     8521 tgctggtgat tgccggactc gacaatctcg cctttctgca tgactaagat tttgttgcaa
     8581 tgctgaatcg tcgacaggcg gtgagcgacg ataatcatcg tcgaaatgtt catcatctta
     8641 gcaagcgaag cttgaatgac ggcttcggtt tcggaatcga tgttggcggt ggcctcatca
     8701 agcgtcatga cggtcggttt gtatgttacc gcgcgggcaa aggaaatcag ctggcgttcg
     8761 ccgctggaga agttgtttcc gcgttccctg acgatatgat ctaagccttc cggaagcttc
     8821 tttaaaacat ggtcgaggcc gacataatcc gcggccttat taatgtcatc gtttgagaag
     8881 gcatcgtttt tcagagtgat gttttcgcgg attgttcccg agaagaggaa gacatcctgg
     8941 agcatctgcc cgacaaaaga gcgaagcgag gaacgcttga tgcgacggat atcgatgccg
     9001 tcgatgagaa tttgtccctt ttgaatgtcg tagttgcgga cgattaactg gagaatggtc
     9061 gtcttgccgc ttcccgtcgc cccgacgagg gcgatcgttt catttggttc gactttaaaa
     9121 gaaattcctt ttaggaccca ttcatcggga atgtaactaa accagacatc ccggaattcg
     9181 atcgagccgc gaaaatttgc cagttcaatc gcgtcttttg catcgttgat ggcgggtttg
     9241 gtatctaaga catcaaaaat cttttcggcg ctggcaaagc cgttttgcaa ggagttgaac
     9301 tgctcggcga tttgctgaat cggttcgaag aattgaccga catagacata aaaggagacg
     9361 aagaggccaa ttgttaaaaa accttcgaag atggaactga cgccgaagta gatggtcata
     9421 agggttgccg ccatggccag aaaatagatg accggccggt aaataccaaa ggtatatatc
     9481 tcgcggaaat aactcttttg caacttttga ttgattgtcg taaactcttt aatttttttg
     9541 tcttcctgat taaaaacctg cgttatcttc attcctgaca gattttccga taagaaggca
     9601 ttgacttccg agacattatt gcggacgctg cggtaggcgc ttcttgaaaa gcggcgaaac
     9661 aaaaccgaag cgacaaagat gaggggaatg acggccatcg tgattaaagc catccgccac
     9721 tcgatgatga acatgatgat ggcgatgacg agcatgaaca aaaaattgcg aatcagattg
     9781 acaatcgtat tggtgaacat ctccgagata ttgccgggat cattagtggc gcgcgtgacg
     9841 agttttccga ccggcagctg attaatttgg ccaatcgata aggattcgat atggttaaat
     9901 acttcagtgc gcagctgatc ggtgattttt tggccgatgc gctggagcat gatactttga
     9961 aaataaccga tgagaattga taatccggtg atgatgagca agattgcagc cccgataaaa
    10021 acaacggtta ctttttgttc gaggctatag gcgggatcga ccatcaaatc gatggcgagt
    10081 ccggatgcca acggcatgac catgttcgta aaagtcgtga cgatcatcag gaggaaagcc
    10141 ccgataaatt tgccggtgta cggtttaaaa tatccgagca gacggacgag gagtttgacg
    10201 tcttgatggg cgacgtggtt ttcgacaaaa tcacgcatgg gtcttctcct ctccgatcag
    10261 ctcttcaagt ttctgcatat tatacatttc tttatataaa gaatttgttt tcaataaatc
    10321 ggcatgcgtt ccgaaaccgg cgaggcgacc ggcgtcgatg aggacgattt tatcgaggcg
    10381 gcggaccgtc gaaatgcggt gggcaatcat caatgtcgtc ttgcctttgc ggacgcgccg
    10441 cagattacgg atgatatctt cttccgtctt ggtgtcgacc gccgagaccg agtcatctag
    10501 gattaaaata gtcggatctt tagctaaagc gcgggcgatg gagacgcgtt gtttctggcc
    10561 gccggaaatc gtcacgcccc gttcgccgag aatcgtctcg tattgctctt taaaacccat
    10621 cacatcgtca tggatgtcgg cgagcttgcc gacttcgaga agtttatctt cgccgatttg
    10681 ctcatatgca aagccgatgt tatttttgat cgtgtcggaa aagaggaagt tgtcctgcgg
    10741 gacatagccg atgtgcccgc gcaaggtttt aagcgataat ttcgcatggt catacggacc
    10801 gaatttaatc atttcttcct cgacattata tacatgtaag agcaattcta ctaaagtcga
    10861 tttgccgctt cctgtcttgc cgagaatacc gaccatctcg ccggctttga tgtcgaatga
    10921 aacatctttg agaatcggtt cttcagcatc cggataggca aaagtcagat ggttgaaagt
    10981 tatcgaacca tccaagaccg catctttgat ggccttgtca ttttcgttga cgatggcttt
    11041 agactcaaga aaggcataga tgcgcttggc ggaggcctga gcctgaccgt tgatgttcat
    11101 aaaccaggag atggcaaata ccggccacaa aagggtgaag aaatacgaga tatattcggt
    11161 cagttcgccg cttgatagat tacctttggc gatcataatt gaaccaaagg tgatgatggc
    11221 caggatgacg aggctgatca tcgaatcaat gatgatgttg acgatgatgg catagcgcat
    11281 gtgatccatt gatttgcggt agacttcctg cgatttgttc ttgaagccga gagcttcggc
    11341 ggcctcgcgg acataggctt taatgacggt gatgccggta aatgattcct gggcaaaatc
    11401 gctggcttgt tcaaaagcct cctggcgaag tttaaacttt tgttccattt tcaattcgag
    11461 aaaaaagacg aggacgccca taagggcaat cggtataaac gaatagaggg tcatcaccca
    11521 gtcgagggtg aacatgcgga ttaggacaaa ggcgccgaga acgacgccgt cgacgagcat
    11581 cagaaggcca aagccaaaga gttcgcggac cgtcccgagg tcgttgataa aatagctcat
    11641 caaaccgccg attttttgct gcgagtagaa gttttggtcg agctttgtgg cgtgcgtgaa
    11701 catctcctgg cggatatcgg tttcgatctt gcgcccggtg ccgaataaaa gaaggcgcca
    11761 caaaaagcga ccgatggtga tgcctccgac gatcaaggcg acggtcaaaa gcggttctaa
    11821 cacttgtgat aagtggctga tcgcccccgt gtcgacgccg tcgatgatga ttttgacgat
    11881 gcggggaatc tcgagttgat accagtcgat gacgaccaaa atcgccactc caaaaaggaa
    11941 agctaatccg tattttaaat aataatggcg aaagtgtttt aaaaagatca tatgttgacc
    12001 gacgcctata ttctatctaa gctgttgcaa aaataaagaa attaaatcaa gatttaagcg
    12061 atttttatgt gaaagcgttt cgaaatcgcg cgctttttta aaaagcgtag cctctgtttg
    12121 cttatgatgg cagtaaaatt tataatgtct atagtatggc caaggataaa catctctctc
    12181 ataatttcgt caaaaatttg ctggccgaag attcgagccg atatccttat acgccggcaa
    12241 aattagctta tttttgcgac ctcatcagcg aaggctataa aagctatcgc atcagctgca
    12301 gccaatcggc cggagtcaaa ccctttgaag aatttgccaa agagattatt caggcccata
    12361 atagcaagtt caacagcatg accgaagatt tcaaacagaa ctacttccgg caagctgatt
    12421 acttacgcaa aatcgtctcg cagaaatgcc aatcactaat caataaaaat gatcccgatc
    12481 atatttttac ttatgtaaat cccaaagacg actcgcgcgt cgaccattta aaagagatta
    12541 tcaacggtta ttattctctt ttgaaggatg ccggcgatgg ctccctcgcc caggcgatgg
    12601 tcaagcggat tatcaatcag atgatttcgg gcatccggct gatttcggtt aagcaatacg
    12661 ccgatgcttt tatcatctgg cgttcccttt tggaaaatgc ttcctatctc aaaatcatca
    12721 ttaaaggcgg taaaaagacg agcaatttgt ttgccgagcg caagaacacg atgaagaagg
    12781 tcatgagact cgctccggcg acaaaggccg aaatggacag cctgcgcgat caggctcaca
    12841 accgggccaa atcggcgaag tggtgggaac atcagcgctt tgtgtgggcc aaaggcgtcg
    12901 tcaacatcaa aggcgagcta tcatcgcgca cattgcagga agccgccgga ctcgacaaat
    12961 actacatcca ctatcaagtg gcatcagtct ttactcacga gcacctgatt aacgaaaatg
    13021 attttcaaaa catctcactt tttgattatg tgattgaact ttattggcga acttttgagg
    13081 agattcgcct cgatatcatc ggtttgttta aacttaaaga cgagcaatta gaatacatta
    13141 acaaacacga aaaaaatctg cggctggcgc tgaagagcac gcatccaaag ttcgatgaat
    13201 tcgcgctgat gatcagctga tttatatcga tttgaaggcg acgcggttgc ggccttcttt
    13261 tttggcttga taaagaagga tatcggcctc ttcaagagcc tcttcgcaat cctttttatt
    13321 tgtcggcgaa cgatacagag ccccgacgct tatcgatagg cgaatatctt tttcttcaaa
    13381 atgaaaagtg cggcccttga tttcctggcg aagcgattcg gcaaactgct tggcttcttc
    13441 ttcgttttgt gcaagatgaa ttaggacgaa ttcctcgccg ccgtagcggg ccaagaaatc
    13501 gccgccttgg agtcgttctt taaatgtttt tccaaggatg attaagactt cgtcgcccgc
    13561 ggcgtggccg tatttatcat ttatcgtttt aaaattgtcg atgtcgatca tcatgatgac
    13621 cacgggagga aatggtttac ttttaaacct ctccttcatc tcatagacga aacggtgata
    13681 agcggcgcgg ttgggaagag aagtcaaaaa gtcggtttgg gcataggtca gaaggtcttt
    13741 ttcggcaccg cgggcgatgg ataaaaaatg ataagcggta cgggcgacgc cgagaatatt
    13801 taagacgatg ttgatgatgt gaaagacgag ggcgagcgga ttagaaagga cgatgagtgg
    13861 cgaaaaacgc aaggcgacga gcaagacgcc gatgaagacg agcgcttcga tcgaggcggc
    13921 gatgagcttc tgaatgttgt tccatttggc aaagacaatc agaacgacca tattgaaaaa
    13981 atagtattga aagccgattt caagtccaaa gacgatgacc tcggccgccg cctgaatggt
    14041 gatgattaaa ataaagataa atgacgccag gccataataa ccgcggcggt taatcaaaac
    14101 ggcgatggcg gtaattccca tcgtcacaaa cgagatggcg gtaatcagaa tgcttcttga
    14161 taagaagaaa agtggaataa tggcaaaaaa agcgaaaaaa gtcgaaagat atatgagatg
    14221 tgaatgctga aagtattcgc gtttagtctt ttcgtcgacg tatttagaaa agcgcatctc
    14281 caatcccctt gttgtagcta tcaagatatc aaaaatactt aagtataaat atatttttct
    14341 aaacatctgc caatttttcc atcatggcgc gtatatattt ttcttttgtt gacacagttt
    14401 ttaatggttg ccataatttg actaactcgt cgacaagagc ggccctcaca aatcctttta
    14461 gcgaaccggg tctggtgcaa gccggtaagg aagaagagaa cagccaacat cttggagtgg
    14521 aagaagaaac caagtagacc ttcccggatc tcgccgttat cgagaatcaa gcgctgaagc
    14581 aattcagaat taaggtggca ccgcgattaa tcgtcctttg catagggcgt ttttattttt
    14641 taggaggatc aagccatgta tcaaagcaaa ctcaatcttc tcgagacgga gaaggccatt
    14701 aaatttatta aagacgagtt tgaacgccgt ctagcgacaa agttgaatct catccgcatt
    14761 tcgtcgcccg tcgtcgtctc aaaagaaagc ggcatcaacg attatctaaa cggctatgaa
    14821 aaacccgtcg cctttcacta tcaaggcgaa gaattggaaa ttattcagtc gttggcaaag
    14881 tggaaaagaa tggctcttaa acgatatggc tttaaaatcg gacaaggtct ctatgctgat
    14941 atgaatgcca ttcgcaaaga tgaaatcatt gatagcatcc attcgattta tgtcgatcag
    15001 tgggactggg aaaaaatcat cacccgcaag cagagaacaa aagaatatct ttttaaaacg
    15061 gtaaaagtta tctattctgt cattcaaaaa atggaacgga tcgttaaccg tcgctatccg
    15121 actttgtttt ttaagctccc ggatgagata actttcatcg atagccaaga attggaagat
    15181 cgttttcctc agcttacgag ccgcgagcgg gagtatcaag taacaaaaga atgcaaggct
    15241 gtttttgtga cacgcgtcgg aggtctcctt aattcgggcg tgattcacga tgggcgttcg
    15301 cccgattatg atgattggga tttaaacggc gatttattgc tctacgatgc ggtgaacgat
    15361 gccgtcttgg aagtctcatc gatgggaata cgcgtcgatg aagtatcact agtcgctcag
    15421 ctaaaagagc gcaatgctct cgaccgactc caatatccgt atcatcaggc gcttttgaac
    15481 ggtcaattgc cgctgactat cggcggcggc atcggccaat cgcgcatgtg tcagttttta
    15541 ttagaaaaaa aacacatcgg cgaagttcaa gcgagctatt ggccacccga gatgctggct
    15601 gctctcgaac aagaaaatat tatcgtcctc taatcttaaa ttagtcgttt tatcactttg
    15661 atggattagg attattttat aatgcataat tttggtggta agagaccatg gaaaaactga
    15721 tcagcgtcga gcagagtgta tttgccctaa gtcaaagtta tccggagatt gtcgagattc
    15781 tcgcctccct cggcttcagc gatataacaa agccaggaat gctcgcaagc gtcggccgct
    15841 ttgtcacatt aaagcagggc gccgcgcttc gcaatatcga tcttcaaatt gtcaaagacg
    15901 cattaatcgc ccatggtttt tcatttaagg agaagaaaaa tgagtgaatt tatcaacaat
    15961 caaagcatac gccaggaaaa gttaaaaaag ctgattcgca gccttcatga cggtaagccc
    16021 ctcgaacagg ccaaacaaga atttaaagaa gaatttggaa gcattacgac cgcggagatc
    16081 accgcgatgg agcaaggcct catcaaagag gggatggccg ccagcgagat tcagcgcctc
    16141 tgcgatgttc acgccgctgt ttttgagggc tcaatcgccg atattcacgc cccaaaagat
    16201 gaagttgacg atattcccgg acatcccgtc cacgtttttc ttgaggaaaa tgagcgtctt
    16261 gaaaaactgc ttaaagaaga aattgaaccg tatttaaatc aaagcggcaa gaccgccatc
    16321 ctcatgctgc gcgttggctt cgaccgcctc aaagaaattg ataagcacta tgcccgcaag
    16381 gagtacttgt tctttcccca tctcgaaaga aagggtatca ccgcgcctcc gaaagtcatg
    16441 tggggtgtcg atgacgagat tcgcgccgac attaaaagcg tcatcgccag cctttcggct
    16501 gttgcaatcg atgaagccgc aacctttgcc ttaattcgga cgacgctcac taaaatacgc
    16561 gacatgattt ccaaagaaaa caatattttg ataccgcttc taaaagaaaa cctgaacctt
    16621 ttcaattgga taagcgtcga attggcaact ccggatacag gctggttcct cgaaaaacct
    16681 aaagtcaaat gggaagaaaa aggatcgagt atcgaaaagc caaagaagaa aaaagaaatt
    16741 gtttcaaaga cggacaccga aattcgtttt gacgccggtt cgctcagcgc cgagcaaata
    16801 aatgcaatgc tcaatgcgct gccatttgac atgacttttg tcgatcacaa aggatacgtt
    16861 cgctacttta cgcaaggcaa agagcgcatc ttcgcccgcc cggccacgat tatcggccgc
    16921 catgtctcga tgtgccaccc gccggcatcg gtgcacgtcg tcgaaggtat catcgaatcg
    16981 ttccgaagcg gccgaaagga ccacgaagat ttctggatta gaatgaaaga tatgttcgtc
    17041 tacattcgct attacgctgt ccgcaatgct agaggtgaat atctcggaac gcttgaaatc
    17101 acgcagaata tcaaacccat cactgaactg caaggcgaga agcgcctcgt cgataaataa
    17161 gctcgcgatt agcaatagcc aaaatacttt aaggtgcgtt taagtttgaa attaatttca
    17221 aaatagcgca tcttttttta acttttcgcg acgatagatg ataaagtaat atttatgaag
    17281 atgcttaaag gagttaacag tgacaaatca aatctcaaaa acgacattcc ttaaatatct
    17341 gaggtgtccg cgcgccgccg cttttgaagg cgatgcggcc gccattgtcc gtgattatca
    17401 gcgcaatctc gcccatatcg gcggtgaaga aaaggcaaag attcttgagg aggagacaaa
    17461 agaaaaactc cgtcagcttt ttatcaggct catcgaaaac gatgaaggtg gagctgaaag
    17521 cgaagcggag attgaatctg gtgattttga cggtttgctc aaagatgatt taacgttaaa
    17581 agcaatgctt tcgacatatt ttaaaatcga acaactgacg gctgagaaag cgaagaagac
    17641 cttcggcgga caagtccttt cgggaatgag ggtgggcgac caggtcatcg gtcagaggta
    17701 tatcaatctt tttaaagacg gttttaattt ccattcgttt gtcgacgtcg ttcaggaaga
    17761 tgatagcgcc gtgcgcatca ttgaagccaa atcgacgaca tcaaagaagt ttgcgaaact
    17821 gggaccgaaa ataaataaag aactctgccc tatttttgag cgttcgccat ttggtgtttt
    17881 gcgccttaaa gaagattttg aaggttttaa acctttcgat aaatattatg ataaccgcga
    17941 aaagttaatg gaccgtttcc gcgatgtcgg ccgctatgtc tatgacctgg cttggcagcg
    18001 ctatatcatt gagaattcga cggatgataa acgcgaatat cgctattatt tagcagtgtt
    18061 aaatagcgat tatgtcttcg acgggcgcca ggacgcaaat ggcgagaaca tctataacgc
    18121 cgatgactta atcgtcttag ttgatctgac gcaagtcacc aaagatatgc tccttattgt
    18181 tgaccgcgat gtcaacacag tcatcgatcg cattaataaa cccgacttca gccgcgttcc
    18241 tttagaaaaa cccaaatgca agattaacgg aagctatgat cggtgcccgt ggctcaaaat
    18301 ttgcatgaag gatttgatgg ttcccgacaa aaattcgctt tatgtctatc gcggcggaca
    18361 caccggtttt ggtcccgcaa gcgaaaactc taaagaaaag gcgcaaaaag aaaagattga
    18421 agaactcatc tggcggggta tcaccaaagc gctggatatt gactacgatt ggcttaatga
    18481 aactcagaag gttcagtatg aggtgataaa aagcggacag ccctttatag ataagaaagt
    18541 aattaaagcc atgcttaagg atttaaaata tcctcttttt catctcgatt ttgaatcgat
    18601 gaatttcccg attcccatgt accggggtga ggtgccttat cagcaatcgc tttttcagta
    18661 ttcgctgcac atcgaaagaa cgcccggtgt ttgcgacaaa gaaaaagata atgtttcctt
    18721 tttgtcgacc ggcaaaaccg acgatcgcga agagttcatc aaatcaatga tcaagaacat
    18781 tcccgcgaat caaggcggaa ccgtcatcgt ttataatgcc atatttgaat caggtcgcct
    18841 cagggagctt attgagatgt ttccgaaata cgctgtcgaa ctcaattcga ttttggaacg
    18901 gatctatgat ttgaaggaca ttttggatcc aagcaaaaaa atcatcgcgg attacgaata
    18961 tttgaagtca aaaccgtgcg cttttaatta ttacaacgaa tcatttcagc gctcttattc
    19021 gattaaaaaa gtattgccgg tatttgcgcc acatctcgat tatgcgaatc ttgatgaagt
    19081 gcataatggt ttggacgctc aagcggcctt catgcgttta aaatatcttg aaggcaaagc
    19141 gtttgatgaa acatacaaga acatgattga atattgtaaa caagatacat gggcgatgtt
    19201 tgaaatcatg caggggttaa gaaaactgat caatgaaaaa taaacgctta ttactaatcg
    19261 ccgccgttct gattttgtct tcctgcgtca acggaggcgg caccggttct gagtcgtcgc
    19321 aggacacgag ttcaggcact tccggcgata cctcatcgaa tacgagttca gatacggcga
    19381 gcgacactgt ctcaagcgtc accggcgaca cttcttctag ttcggagaca ggccggccga
    19441 ctagcggcac ggaaatcatc gatttttatg ctatcaacga ttttcatggt gcgatcagta
    19501 aaaatgacta ttttggtgaa ccaggcctgg cccgcgtcgc cgcctatcta aaagataaga
    19561 aagatgacgc tcccgaaaac agcgtcatta tcagctctgg cgatatgtgg cagggctctt
    19621 acgattcata tcataatcgc ggccgcgtca tcaccgaagc aatggatgat atcggctttg
    19681 attcgatgac gattggcaat cacgaatttg actggggcaa agaatttatc gaagataatg
    19741 aaggttgggc cgactttccg cttctgggtg ccaatatcat ggaatatccc gatacgagcg
    19801 tgaaatcatc agtcggtcag gagtatgcaa ttatcgaacg cggctttctc aaaatcggcg
    19861 taataggtgt aatcggtcag aaacaaataa cttcgatcaa cagccgctat atgcaagatc
    19921 tctattttga agaaccgaca caaatcatca agaacttaag cgctgagctc cgcgaagagc
    19981 atgatgtcga catcgtcgtc ttgtcgattc acgccggaca aggcgaagta gactattcca
    20041 tttcgcaagg gaaatacgtc gatgccgtct tttgttcaca tacccatcaa gttgagcaac
    20101 aagtcgtcaa tggagtgccg tttattcaag gcggttcaaa tggcaaacat gtctcaaata
    20161 ttcgactttc gtttaattat gctaccggac aggtcaatca cgtttcttcg caaaatacat
    20221 atgaaggaca gctgaattct cttactcccg acaccggagt gcaggcgatt attagccagt
    20281 tcgcccagtc ttcggattcc gataaaaatc aagtcgtcgg tacgctgacc aataatttga
    20341 atcggtatga cacacttcct aatatggcaa attatacagc ggctatgaaa gccgttgaaa
    20401 atggctatga catcgacttt gccatgacta atgtcggacg cagcaacatt cccggcggnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ccctatgatt acaccggctc
    20581 gcgttacgag agtctgacca tctagaattt ttgtcgatct ttttgcgcta aataatgata
    20641 tttgccgtat tttattataa aatagtcaag taatttcctt taaaggggag tagcgaagcc
    20701 ttccttccgt caagacggca tcacgcccgg aactaggcca gtaatgagca agacctttaa
    20761 aaccttaaaa caaaggagag cttattatgt acgagacaaa gaagatcgat gaactattag
    20821 tcgaacacgc cgtcgacccc gcagttggcc taacgagcga agaagcttgt cggcgactgg
    20881 aaaaaaatgg caagaataaa ctagttgaaa aaaagcgcag aagcattttt ttgacctttt
    20941 tattgcagat taacgatccg atgatttatg tccttttggc ggccgccctc atttccgcct
    21001 tggtcaccat ctttggcgaa ggccacgatg actgggccga tgtcatcatc attttagctg
    21061 tcgttttatt gaatgcgaca atcggcacaa ttcaggaagt caaagccgaa aatgctcttg
    21121 aagctttaaa aaagttatca tctccgaccg cgaccgttag gcgcgatggc aaagtttttg
    21181 aaatcaaagc tgaagacctc gtcctcggcg atatcgtcat cctcgaagaa ggccgctcca
    21241 tcggcgccga tatgcgcctt ttaaaagaat acaatctcaa aaccgatgag tcttctctga
    21301 ccggagaatc gactcccatc gaaaaagatt atcaagtcgt tttctctgaa ccggtcggcg
    21361 tcggcgaccg catcaactcc gtctatatgt caactccgat tgtctacggc catggcgaag
    21421 gcatcgtcgt cgccaccggc gaaaaaacgg aaatcggcaa aattgccaag atgctggctg
    21481 atgaaaaaga agaggccacg ccgctacaaa agcgcttagc cgatctatct aagattctcg
    21541 gcattatcgc catcgtcatc atcgtggcca tctttgccgt cgccttaatt caaaaacgcg
    21601 atcccattga gatgtttata accgccatct cattagcagt tgccgctgtt cccgaagggc
    21661 ttccagccgt cgtcacgatt gtcttggctc tcggcgtgca gagaatggtt aaagtcaaca
    21721 ctatcgtccg ccgtcttccc agcgtcgaaa cgctcggtgc cgtttcggtc gtctgctcgg
    21781 ataaaaccgg cactctcacc cagaataaaa tgacggtcgt cagagctttt gtcgaccatg
    21841 aaatcatcga ttccgaacat ttcaaaggcg atagtgtctc cttcctggcc cggggcatgt
    21901 cgctttgctc taacgcctcc atcgatagcg gtgtctatgg tgatcccacc gaaatcgctc
    21961 tcgtcgaatt cggcaataaa ctcggcttgc gcaaaaaaga cctcgaaggc gtccatccgc
    22021 gcatcaatga gctgcctttc gattcggttc gtaagatgat gtcgactcat cacgagcacg
    22081 aaagcggagc catcgtcttc accaaaggtg ctctcgattc agtcattaaa catacaaccc
    22141 atatttttat taacggccaa gtccggccaa ttaccgaaaa ggatttcaaa caaatcaatg
    22201 aaggtagcgc ctacatggcg cgtaatgcct tgcgcgtatt agctttggct tataaggaaa
    22261 gttctgaatt aagcgaagat aatttgactt ttgtcggcct tgtcggcatg gttgatccgc
    22321 cccgcccaga agccggcaaa gcggttgcca tctttaagaa agccggcatc gtcaccgtca
    22381 tgattaccgg cgatcatcgc gacaccgcat tagccatcgc ccgcgagttg aaaatcgctc
    22441 atgaggaatc cgaagttcta tccggcgatg agctttcgaa aatgagtttt gaagaactcc
    22501 agcaagcggt gaaaaccgtc cgcgtttttg cccgtgtctc cccggaaaat aaagtcagca
    22561 ttgtcaaagc gatcaaagcc aatggctaca tcgccgcgat gaccggagac ggcgtcaatg
    22621 atgctccatc gctaaaagca gccgacatcg gcatcgccat gggcatcacg ggcacagatg
    22681 ttgccaaagg cgccgctgat atggttttga ccgacgataa ctttgcgtcg atcgaaaagg
    22741 ctgtcgaaga aggtcggggc atttatgcca acatcaagaa gaccgtctac tttctccttt
    22801 cgagtaatat cggtgaagtc atttcgatgt tcgtcggcat cgtcttagga ctcccggtac
    22861 cgcttatcgc catccatatt ttatgggtta acctcatcac cgactccctg cccgccatcg
    22921 ccctcggttc tgatgaaaaa gccgataata tcatggaaga caaaccccgc gacgaaaaag
    22981 aatcgctctt tgcccgtggc ggatatttct ttatcatcac ttacggtatc gtcatcgcca
    23041 tcatcaccct gttcggcttc ttactctttc ccacgattca tatcgtcgaa agcggcctgc
    23101 cctttacctt ggccaatttg atcgcggtga ccaatgaacc gacgatgctt cgcctatctc
    23161 agagcacggc cttcagcgtc ctcggcgttt ctcagctctt ccacatgatt ggcatgacca
    23221 acaccgaaaa gagttttgtt catgttttca aatcgaaaaa ctggctcctt ctcatcgctt
    23281 ttgtctttgg tttgggaatg caaattctca tcaccgaaat tccttttctc gaagatttct
    23341 tcagcaccgc ccagctgacg cttgtcgaat ggctctatgt catagctctg tcagtcgtgc
    23401 ctcttgtcat tcacgagatt atcgttttaa ttaagttcat taaaaagcag atcaaaaaaa
    23461 gaaaattggc cagcggcaat taaacgtatt tatcacttga aaagcggctt gatatatttt
    23521 actattatta agccgcgcat gccgacttag ctcaatccgg cagagcaact gaatcgtaat
    23581 cagtaggttg taggttcaat tcctatagtc ggcaccaatc ttttaatcca gttggatgtt
    23641 tagctcagct gggagagcat ctgtttgacg tacagaaggt cgggggttcg atcccctcag
    23701 catccaccat ccgttataaa tggcctccgc aagaggcttt ttattttgct ggtgacaaac
    23761 gatttatatc taacctataa tatgggcgat gcatctgtag ttcaatggta gaacactagc
    23821 ttcccaagct aggtacgcgg gttcgattcc cgtcaggtgc tccagtaccg aaacaagaga
    23881 attcaccgaa tgcataaggt gggttttttc ttttttatgg tgacgagtaa aagcagaaga
    23941 aaccaggtgc aattcaggct ataaatgact ttttacggat ttttttcaaa tattcaccta
    24001 attcgcgatt aaaaaaatat ttttagaatt atctcttcaa aagccccctc atttcaccct
    24061 tttcttctcc gccattgttc tgcgctttaa tttagtaatt aactttgcta aaatacaaga
    24121 tgaacaaaaa caaaacgggg aacgaatatg gaagattatc gccagaaatg tattaatttg
    24181 cttaaagaaa gaggcgttac gctgaatgat attgctgatg cggcccgctt tttacaggcc
    24241 gattaccacg tcgatttgct caaggaagaa ttgctcgatt cggttttaag cgttcttgcc
    24301 aaacgcgagg ttcagctcgc catcatgac
//
LOCUS       DAOSSL010000014        23911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2294_length_23911_cov_12.882964, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000014 DAOSSL010000000
VERSION     DAOSSL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 23911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23911
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2294_length_23911_cov_12.882964"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLH17_02425"
     CDS             <1..717
                     /locus_tag="PLH17_02425"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOR20667.1"
                     /translation="IVRILDNFTELTAELTTELTARKKQYEFYRDELLVSDNSNKTIK
                     LSDLFDIRNGYTPSKDNPEFWDNGTIPWFRLEDINDNGRILYDSYQHISDKALKRNGL
                     FKDNSIIISTSATIGEYALIKVPFLCNQRFACLTIKDEYKNNFSIEFLYHYCPLLSQY
                     CKNHLNKGNFASVDMTKFGDFLFPILDLNEQQRIVDILDKFDAYCNDLSIGLPAEIAA
                     RQKQYEYYRDKLLTFKELQA"
     gene            717..3797
                     /locus_tag="PLH17_02430"
     CDS             717..3797
                     /locus_tag="PLH17_02430"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOR20668.1"
                     /translation="MAQYTVISQSNEETVVTEYKPITSRSDAYQSEAALEKEFIRMLG
                     EQSYEFILIREEKNLIDNLRKQLSLLNDYQFSDNEWKQIFNSCLCKPNAGIKEKTEII
                     QENHIFPLKRDNGETKNIKLIDKVNIHNNKLQVINQYEVSDGTYKNRYDVTILVNGLP
                     LVHVELKRRGVAIKEAFNQINRYQRDSFWAGSGLFEFVQIFVISNGTHTKYYSNTTRF
                     SHVKDINKTQKTDKTSNTFEFTSFWADAKNKIIPDLIDFTRTFFSKHTLLNILTKYCV
                     FTAEDKLLVMRPYQIAATERMLNRIEVATNYKWFGSIKAGGYVWHTTGSGKTLTSFKA
                     ARLTSKLPYIDKVIFVVDRKDLDYQTMREYDRFEKGAANSNTSTAVLKRQLEDPSSTI
                     IITTIQKLSIFIEQNKKHPVFDKKVVLIFDECHRSQFGDMHTAIIKNFNKYYIFGFTG
                     TPIFSVNSSTGGNPHLKTTSQAFGDQLHVYTVIDAINDRNVLPFRVDYIKTMDQDDAI
                     EDEKVKDIDREAAFNAPQRISLVTQYILDHYNQKTYRNEKTYSQSVISNVHDVLKGKE
                     EVREKIRLSGFNSIFATASISSAKLYYAEFKKQIKELPDAKKLKVAIIYSYGANEDEI
                     DGIIDEENPEDTSRLDQSSRDFLEASIKDYNEMFHTNFSTQGEGFQNYYRDLSLRVKN
                     KEIDLLIVVNMFLTGFDATTLNTLWVDKNLKMHGLMQAFSRTNRILNSIKTFGNIICF
                     RPLQKKVDESISLFGDRDATGIVLLRGFNDYYNGYTDNEGKYHKGYAELIEELLENYP
                     LSDPRIEGEDNQRNFLKLFGVILRLKNLLASFDDFIGHELISDIDLQDYVSRYLDLKP
                     SAKPGELKNINDDIVFEIELIRQIEVNIDYILMLVKKYHDSHCKDHEIIIAIQKAMDA
                     SPELRSKRDLINHFLTGINEIDDIIAAWHDYISEQKEKEIKRIVEEEGLKNDNTREFI
                     SNCLKDGYVKTIGTKIDEIMPPMSRFEGKHRAEKKTRVINLIITFFDRFFGIGE"
     gene            3893..4426
                     /locus_tag="PLH17_02435"
     CDS             3893..4426
                     /locus_tag="PLH17_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20669.1"
                     /translation="MEKNVIVNALYEQYAKVVKEKRVAQPELEFSFISGSIESCGSDN
                     CFISEHPESVYGFAVYVEDHEKESMYKKLIETNVGVRDKREYRLANANSVNEWSPLAK
                     SKYYPLYWGKSQCLFKRIIDHTKSAVGVGSLWLHRNQALHGKKVIFGTIPCYFPKEVE
                     ESLQKAYKQVLKTHREP"
     gene            4941..5060
                     /locus_tag="PLH17_02440"
                     /pseudo
     CDS             4941..5060
                     /locus_tag="PLH17_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557776.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            5084..5725
                     /locus_tag="PLH17_02445"
     CDS             5084..5725
                     /locus_tag="PLH17_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20670.1"
                     /translation="MQANDKVDQITPKISTLTIVLAFLSFLFVVLACLLMYFYHMAVW
                     TLIVNFQIIYFVWVLIFALAVGLLIIILSGVTMRKEKNGNKLVLLPPFLVVGLAIFSM
                     SFGLFEKFAYERHYTFSVEKWAVASSDERSVYLDSFLEQYDLYTFNDQMIVATLDEPD
                     EKRTIELQTDPVQFGGYSYVYDLGFVRDYMDPSFFEITTDQSGVVSYYHIYST"
     gene            complement(5754..6875)
                     /locus_tag="PLH17_02450"
     CDS             complement(5754..6875)
                     /locus_tag="PLH17_02450"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOR20671.1"
                     /translation="MMKIVIASDSFKGTLSSLDICHLFKDELVKYPLIKAAYLPIADG
                     GEGSLEAIAFSLKGRYINLTAKNPYFVDIDSRYFLDDNNVAYLETASTAGLTLIEDLN
                     PAKTTTFGLGQQVLDAINKGGKKIYIFLGGSATNDGGCGLFAALGTKFYNGRNKEFIP
                     VGSTLKDIVRIDNSFVDKRLEGIEIIGMCDVDNPMHGPQGAAYVYALQKGADNKMVIE
                     LDKGLKHLAAIIKKDLHIDVASNPGAGAAGGIGAGILAFAKGTLKSGIETILDLIAFD
                     NAIEDADHVISGEGRLDEQSFYGKTIKGIATRCQRNNKKLILIVGSSNISLKEAQEKC
                     PYIETIIETNPDHLPFADIKSKASSMYKDAMHRLISMIR"
     gene            6965..>8202
                     /locus_tag="PLH17_02455"
     CDS             6965..>8202
                     /locus_tag="PLH17_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOR20672.1"
                     /translation="MRTVKAFFAEIANFIVRAKKPLIVLGSFLAATFLCVGLADGIQK
                     DWGNIQVTSGMINATTVDNEDYSMGYKLYVPKSATQGSPAPAVLCLHGYQNDHETSAG
                     YALEIARHGYVALAIDEFGHGATTIGIAARGWVNHKVSNVNYGWESEADKTYSFISGP
                     DRYKVMMNFSNIDFFIDKYSKAIDPDTGDVIVDENYIKDASMGGIAAYTWLATQNFVD
                     KTKMAVTGHSMGTWAAWSVSAAMCDAQIEGVDVTPRATILQAGEIFKTTRDGDGRMAY
                     DSDEDSVADIYWNNPLLLSAKYDEFNMFRDYQSPPNGDLVISGEPSRNFLNPGTGEEP
                     EWNKTFHEDFALGTSVRRNLYNTNHRLLTHHRAAIADTLEWLSLSSGVSLSIDKTNQT
                     FLVKEVLVFVAMILAMASAVT"
     assembly_gap    8203..8302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8303..8848
                     /locus_tag="PLH17_02460"
     CDS             <8303..8848
                     /locus_tag="PLH17_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20673.1"
                     /translation="GKSAIVVFAGVIWMYLQVVISQALFQLDFRFIWPFFKAFNGARF
                     LQFLVYLPIFAIFFILNNSKIFAGNRLPGTNETGFLSFIKVWWKYALCMVGGILFIIL
                     LEYIPFFAGIGPGADLVFSTTFGGPFMSLLIVFAPQVIVFSLICTYCYKKTGNVFVGA
                     MLVAILACWIITGGSAMMYSF"
     gene            complement(8922..9533)
                     /locus_tag="PLH17_02465"
     CDS             complement(8922..9533)
                     /locus_tag="PLH17_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOR20674.1"
                     /translation="MKEVFIYLENGYADWEISHIGSQITSSNKYLVKYVAKTKDPVVS
                     MSSLCMIPDYAADEVLNSEIGKFAMLILCGGMYWSKANFINEEAKNLVDLFVKNNKPV
                     AAICDATAFLACHGYLDDTPHTGNSIGHIIQVCPSYKGHSHYINEQCVASESFITANG
                     TGEYEFVREIVKRLEAKPAQEIDTWYHFRKKGLAAYINEQSKK"
     gene            complement(9668..>9931)
                     /locus_tag="PLH17_02470"
     CDS             complement(9668..>9931)
                     /locus_tag="PLH17_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20675.1"
                     /translation="DFDIWLVFIYAIPLSSIVLLVFSLLWGNNRLSFAFVTALSVGLT
                     LSIYLSLFKFYPHIWYIFIALVPIEILALFWFVFRKIRKVPTE"
     assembly_gap    9932..10031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10264..11109
                     /locus_tag="PLH17_02475"
     CDS             10264..11109
                     /locus_tag="PLH17_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOR20676.1"
                     /translation="MKIAISTETTVDLTKDLLKEFDIHTVAFTIVMGEEQVVDGEISP
                     EEIFSFHERTKILPRTSAVNEYQYETHFQELLKSYDAIIHISLSSTLSVAYTNASNVA
                     KKLNNVTVIDSRSLSTGIALLCLHAQKLVRAGERLSNIINSIYERIPFVQASFALEKV
                     DYLYKGGRCSLLSFIGANLLGIKPQIIVKDGKMIPGRKYRGKMIRVVKQYVEDTLQYY
                     HNPDLEQVFITYTTADFEIIEMVKTMLVERGFKRIEVTRAGGTISSHCGENCLGILYI
                     NDGDR"
     gene            complement(11175..11627)
                     /locus_tag="PLH17_02480"
     CDS             complement(11175..11627)
                     /locus_tag="PLH17_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20677.1"
                     /translation="MKKLIPFVNILVLALLGIVWPLGAAVALYVPNYTGTGVASNIGN
                     FYQVVWGHGSNVASVVVIVAFLLLAVGSLLVIPAFIPFKFQRIILGAIAVFFVVAGII
                     FLLMPTLVGQVNPNYPASVKLGGSVIGMSVLLLSAGVLEGLMAFAPSK"
     gene            11822..12847
                     /locus_tag="PLH17_02485"
     CDS             11822..12847
                     /locus_tag="PLH17_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOR20678.1"
                     /translation="MKKVYNTLASLLSVLALASCAGSPEALTYEHYKKRDLDYQENFR
                     VLQMTDIHFGLATNFAEEWVYFDALIGLAEPDLMVLTGDMFMNASVDVVNQLYNYMDS
                     KDIPWTVTFGNHDRQGFYTPQFIEGQATYDSYDNCLYVNPDDNVNGHGNHYINIVSGT
                     DVEWQVIMLDSGSYHSLGATYDYDVIHEDQIAWYEDVIKETNKVQFDVDDFALVPPAN
                     VIPSVAFFHIPLFEYVDAYEAWEASGFDEDMGSGVYQDSGIWHGYYNSGFFAKAKELG
                     STRGMFVGHDHTNNFAIEYQDIVLSYGVKTGRGIYHDPEMIGGQVITLGDDGTIDIER
                     LFVAYGE"
     gene            12852..13793
                     /locus_tag="PLH17_02490"
     CDS             12852..13793
                     /locus_tag="PLH17_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20679.1"
                     /translation="MKTKTLFLVTNLLLFFFAGISTFFFVMVLFTDAINNFRMVLAYL
                     PLYLTLILPIYALIAVNRIAKVESDNLKLRKIKINSLVFGSISAFALIYGFINVFVSF
                     GGNFLSGGPSKYFPLSFLVLDFLFALIAVYILFIFRSEKCEKEAAPQKGALKNVGGVL
                     KTIFLLFALYFLGTFAFGFYSFDLSLEHVGGTLPVYLLMILPSLMIVANEIVKGYVLF
                     EKQKFARFILGLSGLGLTLVLGVWMFIYMNAKPNFVVDALTASFPLDFAISINLGPIL
                     VLILSLIPGLYLTIAYLLPNYCPIYKRWRAKNDNPTK"
     gene            13941..14399
                     /gene="rimP"
                     /locus_tag="PLH17_02495"
     CDS             13941..14399
                     /gene="rimP"
                     /locus_tag="PLH17_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOR20680.1"
                     /translation="MAVKDVVSALIEPQLKILGYDLASVSFRKEGANYILRVVIDKDG
                     PVSMDDIVRAGDLISPLLDEADPIKTSYMLDVTSFGAEKPIKLERLERYLNERVNLGL
                     REAVDKKNALEGELVSLTKEEVTIKVDNKKKGNVTIRRDLIANARLAIKF"
     gene            14412..16010
                     /gene="nusA"
                     /locus_tag="PLH17_02500"
     CDS             14412..16010
                     /gene="nusA"
                     /locus_tag="PLH17_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOR20681.1"
                     /translation="MINAAQFFEALDQLEAQKGISKESILVALKEAMKKALVKQLGGG
                     DDAVIDVIIDEETAKIEMYVIKKVVAEVADDYLEISLDEARKFDQKYNLGDDYKIYIN
                     IHDLSKLFAMTVKSVLHQKLAEAEKAALYVTYKDKIGEMITGVVEKADERSIIVNIGR
                     TSVYLSNRELIGEETFNVGDTIRLYVSDVSSTEKGAQIVVTRSDPGFLRRIFEEEIHE
                     IYDGTVIIKDIAREAGTRSKVAVFSHDPNVDPTGACIGHDGGRVKKILGQLGNARDKE
                     KIDIIQYQEDPALYIVEALRPATVVGVHLDKEEHAALAVVKDEQLSLAIGRKGANARL
                     ANRLTGWKIDIKEEAMAKEANLEYFNVEELHQRHADQLKAEAYQRYIDSIKPTPKDVV
                     VETPVASEPAAAPVSDVTPLVEAKPVVEEAPKVVEEPIKIKITTDLETLERELESQKE
                     KNKQAAPKAKRPRKISDDEVKSEAKDEKPYVAPEADNKPYMQIYSEEELAELEKEAAK
                     HPDELIDEEEIDYDEYDAYYEDEE"
     gene            16042..16299
                     /locus_tag="PLH17_02505"
     CDS             16042..16299
                     /locus_tag="PLH17_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOR20682.1"
                     /translation="MRMKKIPLRRCLASNQMFPKNELLRIVKTPAGEIVIDRTGKVNG
                     HGAYLRKDAEIFALAKKRRLLDKAFGMAVSDEVYDSLSNYL"
     gene            16296..16604
                     /locus_tag="PLH17_02510"
     CDS             16296..16604
                     /locus_tag="PLH17_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOR20683.1"
                     /translation="MNNRVSGYLGLANRAGKALVGEAITKVWSKRKIYLLIMALDAVS
                     KTQEKLAKLAREQNVTTIYAYRKAELGHALGFAEVSTVAISDKGLSVQLISVIKEDNK
                     "
     gene            16601..18433
                     /gene="infB"
                     /locus_tag="PLH17_02515"
     CDS             16601..18433
                     /gene="infB"
                     /locus_tag="PLH17_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOR20684.1"
                     /translation="MNDKNKKRNRVANVPAGNTPRKGENAKVNKGVFVYTKCMSVGEL
                     AKQLEINSSDIIKYLFMNGKVITINQTLDDEAIGTVCLQFGFDFRKDKEVSEENFEEL
                     EIVDDPKDLKSRPPVVTIMGHVDHGKTTLIDAIRSSHVVETEVGGISQAIGAYQKEIK
                     GQKITFIDTPGHEAFTAMRSRGASITDIVVLVVAADDGVMPQTVEAINHARAAKVPII
                     VAINKIDKPGADAKRVRNELVAYDIIPEDYGGNNIFVEISAKKGIGIDDLLENIILVA
                     EMQELKANPRRYALGTVIEANLDKGEGPKATLLVQNGTLNFQDYVVLGSAYGKIRRMT
                     NEYKKILKAAGPSTPVSIIGLSEVPNAGDSFMAFPSEKQARDIASKRRLAKESEGRGG
                     SSAASLDDLYNRIHEGEVENINVIIKADTTGSAEAIKGALEKLETEGVKVNIIASTAG
                     AITESDILLASASHAIVYGFNVRPNALVKAKADEESVEIRLHRIIYALIEEMEAAMRG
                     KLKVQMVEKITGAAEVRVIYKVSKLGTIAGSYVTNGTLKHGQSVRLIRDGVVVYEGKI
                     ASMKRYKDDIKEAREGYECGIVLDGFNDEKEQDIVEGYEMVEKK"
     gene            18437..18796
                     /gene="rbfA"
                     /locus_tag="PLH17_02520"
     CDS             18437..18796
                     /gene="rbfA"
                     /locus_tag="PLH17_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOR20685.1"
                     /translation="MANRTERIQSIIAKNISEILQFEVKNKSVGLVSVTEVRVSSDFS
                     YAKVYVSFIGAKHPNVNLEALNAAKGFVRSSLASKLDIRKVPEISFILDDTFEKRDRI
                     EKILKEEAETLNKLRGK"
     gene            18798..19727
                     /locus_tag="PLH17_02525"
     CDS             18798..19727
                     /locus_tag="PLH17_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20686.1"
                     /translation="MSNGSDIVVETKGLTKKYGTTLALNNVSISVHRGEIYGFVGRNG
                     AGKTTLIRVLTGIANPTSGSFTLLGAATKNEQKEARKHIAAMVETPALYMNMTAHDNL
                     RTTLILKGLKDESLIRSRLEEVGLSDVIASSKKAKDFSLGMKQRLGIAMALLGDPELL
                     ILDEPTNGLDPEGIREMRELLVNINKSRNITMIISSHILGELSKLATRFGFIDKGVII
                     NEISSDDLFNKVSKSVIIKTDDNVRALAILNGAYQNRVTSENGSIHVADVNNSSEIVK
                     LLVKSDINVVQFREQENGLEDYFINLIGGNHHD"
     gene            19720..20583
                     /locus_tag="PLH17_02530"
     CDS             19720..20583
                     /locus_tag="PLH17_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20687.1"
                     /translation="MTKLLKLQLFRALKDKGTIVFTIITLVLGILSITFNFLISDSIG
                     QGGATLIGDLFAISSLINSSYSLSSTPMVLLIIMGVVLLYKESGWGTIRNQVVAGYSK
                     IQIYFANFLAMLIILFTILLTYQLLTLGLGFIFGFELGIETAAEVGIFFRKYALNLVI
                     FITEVAFMTFISLMFQNIIAPVFLVVILPIFVGAILQTVVPLVVILNQDNAAQIIGGF
                     EYVIWYQNTSLSISGFTYELTSGVGSLMGATLYDTSTLGFILKTIGTNLGLNGIFLGL
                     GSYLFSRKDLK"
     gene            complement(20589..21782)
                     /locus_tag="PLH17_02535"
     CDS             complement(20589..21782)
                     /locus_tag="PLH17_02535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20688.1"
                     /translation="MRAIRKVLASIRNADLRFSLINQGDKIVVGVSGGKDSLVLLYAL
                     NLYRKFAQCDFEIIPVMLDLGFASFDPSAIQEYVRTLGMELVIADASTVYPILAKHTK
                     EGGHLPCSICSRMKKAAINKVAKGLGAKKVAFAHHAEDAIETLFMNELYGGRIATFAP
                     KMHLQKADIDFIRPLILCRESEIEKTAREEKFPVMKSPCPSDGFTTREDVKNLLAGIY
                     KKYPDAKENFLTMLYNYEQEDLWGKEIFYKIEGSDLSLKPVLSKEDALIMAKIRTDVF
                     LIEQSIPPQDEFDGSDEGQEHLLIYKDQTPIGAIRYTFDEERLIHLSRICTLKQYRGF
                     GYTKKALSFLIEMLLAKTNPLIFTLGAQVQALGFYEKLGFKKVGDVYLDANIPHFKMV
                     LKRDK"
     gene            21824..22687
                     /gene="truB"
                     /locus_tag="PLH17_02540"
     CDS             21824..22687
                     /gene="truB"
                     /locus_tag="PLH17_02540"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOR20689.1"
                     /translation="MESVNQKSGILLLDKVIGPTSRQVVNGVTSLLRTKKIGHVGTLD
                     PFASGLLILTVNQATKIGAYLEALDKTYIAHLKLGIQTDTGDLTGETIATQKVPNNLD
                     FAIINDVLHSFLGKRLQIPPMYSALKREGKPLYRYAREGLEVERQPREIEIFDIELLS
                     FENDVIEFSARVSKGTYIRTLGEEIAVALHTLGHLISLRRKAIGNFRVEDAEPLKKVA
                     FERLISIRKALSHLPVFEVDEASIKDIMNGKPVKLPEIGQIVLLVSDEKALALYEKRD
                     DGLYYSRRGLF"
     gene            22688..23581
                     /gene="ribF"
                     /locus_tag="PLH17_02545"
     CDS             22688..23581
                     /gene="ribF"
                     /locus_tag="PLH17_02545"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOR20690.1"
                     /translation="MKTILLELENLPRFASPISICLGNFDGVHIGHQQVIKKAVLDSG
                     TEVAVLTFDAPLGSVIDGRKAKQVLTSVSDRFQIIHRLGADYLFVLHLDEKLLSMSAE
                     DFIVKVLKKLNVAKVFAGQDYRFGHEQAGDIELLSKHFSVTSLPLFDVEHHKVSTSQI
                     IAFLKQGKINKANELLGRHYQMHGTISEGLHNGKTIGFPTANLKLSAPYVIPKFGVYK
                     TICYIDGFPHLSLTNVGLHPTIQQLDMPSIEVHIPNYEGEDYGKTMYLEFIAFLRPER
                     RFTTLDELKAQISRDIVSIKP"
     gene            23706..>23911
                     /gene="rpsO"
                     /locus_tag="PLH17_02550"
     CDS             23706..>23911
                     /gene="rpsO"
                     /locus_tag="PLH17_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOR20691.1"
                     /translation="MALSKERVAELIKEYGKNANDSGSPAVQIALLTEQITNLTAHLK
                     ANKKDHHSRRGLLIAVGKRRSLLD"
BASE COUNT         6796 a         5454 c         5178 g         6283 t
ORIGIN      
        1 attgtacgaa ttctggacaa tttcacagag cttacagcag agcttacaac agagcttaca
       61 gcaaggaaga agcaatatga gttttataga gatgaattat tagtttccga taatagtaat
      121 aaaacaataa aattatctga tctttttgat attagaaatg gctacacccc atcgaaagat
      181 aacccggaat tttgggataa cggaactatt ccttggttta gactggaaga tataaatgac
      241 aacggaagaa tattatatga ttcgtatcag catatatccg acaaggcctt gaagagaaat
      301 ggtctattta aggataatag tataattatt tccacgtcag ctacaattgg tgagtacgct
      361 ctgattaagg taccgttttt gtgcaatcaa agatttgctt gtttgaccat taaagatgaa
      421 tataagaaca attttagcat cgaattttta tatcactact gtcctttgtt atctcaatat
      481 tgcaaaaatc atttgaacaa aggcaatttt gcttctgtag atatgacaaa gtttggtgat
      541 tttctcttcc cgattcttga tttgaatgaa caacaacgca tcgtggatat tcttgataaa
      601 ttcgatgcgt attgtaacga tctatcaata ggtctccctg ctgaaattgc ggctcgtcaa
      661 aaacagtacg aatactatcg tgataagcta ctaacgttta aggagttaca ggcataatgg
      721 ctcaatacac ggttatttct caatcaaatg aagaaacagt agtgacggaa tataagccga
      781 tcacttctcg ttctgatgct tatcaaagcg aagctgctct tgaaaaagag tttattcgca
      841 tgctcggaga gcaatcttac gagttcattt tgattcgcga agaaaagaat ttgattgata
      901 acctaagaaa gcaattatca cttctcaatg actatcaatt ctccgataac gaatggaagc
      961 aaatctttaa tagctgtctt tgtaaaccta acgccggaat caaagaaaag acagagatca
     1021 ttcaagaaaa ccatatcttc ccgctaaaga gagataatgg tgaaacaaag aacattaagt
     1081 tgatcgataa ggtcaatatc cataacaata aactccaagt cattaaccaa tatgaagtga
     1141 gcgatggaac ctataaaaac cgctatgatg tcacgatttt agtcaatgga ctgccactgg
     1201 tccatgttga actaaaaaga cgtggtgtgg ctattaaaga ggccttcaat caaataaaca
     1261 gatatcagag agacagcttc tgggctggca gtggtttatt tgaattcgtc caaatctttg
     1321 tcatctctaa tggtactcat acaaagtatt attcgaatac cactcgtttc tcccatgtta
     1381 aagatattaa taaaactcaa aagacggata aaacaagcaa cacctttgaa ttcacttctt
     1441 tctgggcaga tgcgaagaat aagattattc cagatttgat tgacttcact aggacattct
     1501 tttcgaagca cactttacta aacattctta ctaaatactg tgttttcact gccgaagata
     1561 aattgctcgt gatgcgtccc tatcaaatcg ctgcaacgga aagaatgctt aatcgaattg
     1621 aagttgctac aaactataaa tggtttggat ccattaaagc cggtggatac gtctggcata
     1681 cgaccggtag tggcaaaacg ctcacttcct ttaaagctgc tcgactaaca tcgaagcttc
     1741 cttacatcga taaagttata ttcgttgttg atagaaaaga cctcgactat caaacgatgc
     1801 gggaatatga tcgttttgaa aaaggtgcgg caaactcgaa cacctctaca gctgttttaa
     1861 agaggcaact tgaagatcca agtagcacaa tcatcattac taccattcaa aaactctcta
     1921 tcttcatcga acaaaataag aaacatccag tctttgataa aaaagtagtg ctcatctttg
     1981 atgaatgcca tcgtagccaa tttggcgaca tgcacactgc cattatcaaa aactttaata
     2041 aatactatat tttcggattc actgggactc caatctttag tgttaattcc tccacgggtg
     2101 ggaatcctca tttaaagaca acttcacagg cttttggtga tcaactgcat gtttatacag
     2161 ttattgacgc cattaacgat cgaaacgttc tccctttcag agtcgattac attaagacga
     2221 tggatcaaga tgacgcaatc gaagatgaaa aggttaaaga tattgaccga gaagcagcgt
     2281 ttaatgctcc tcaaagaatc tccttagtca ctcaatacat tcttgatcac tataatcaaa
     2341 agacatatcg taatgagaag acttatagcc aaagtgtgat atcgaatgtc catgacgtgc
     2401 ttaaaggcaa agaagaagta agagaaaaga ttcgcctcag tgggtttaac tctatttttg
     2461 cgacagcttc catatcctct gctaaactct actatgctga attcaagaaa cagatcaaag
     2521 aacttccaga tgccaagaaa ctcaaggttg ccatcattta ttcgtatggg gcgaacgaag
     2581 acgagatcga tggcattatc gatgaagaaa acccagaaga tacttctaga ctcgatcagt
     2641 cctcacgtga ttttcttgaa gcttcaatca aagattacaa cgaaatgttc catacaaact
     2701 tctcaactca aggagaagga ttccaaaact actatcgaga tctctcctta agagtgaaga
     2761 ataaagagat tgatctactt atcgtcgtga acatgttcct cactggcttt gatgccacga
     2821 ctttaaatac tctttgggtt gataagaacc ttaagatgca tggtttaatg caggctttct
     2881 caagaacaaa ccggatcctt aactcaatca aaactttcgg aaatattatc tgttttagac
     2941 ctcttcaaaa gaaagttgat gaatcgatat ccttatttgg tgatagagat gcgacaggca
     3001 tagtcttact aagaggcttt aatgattact acaacgggta caccgataac gaaggtaagt
     3061 accataaagg ttatgcagaa ctcatagaag aactattaga aaactatccg ctctcggatc
     3121 cacgaattga aggcgaagat aatcaaagaa actttctaaa gctcttcggt gtcatcttaa
     3181 gacttaagaa cctacttgcg tccttcgatg attttatcgg ccacgaactt atctctgaca
     3241 ttgatttaca agattacgtt tctagatacc ttgatctcaa accatcagcg aaaccgggcg
     3301 aattaaagaa tatcaacgat gatatcgttt ttgaaatcga actcatcagg caaattgagg
     3361 ttaatatcga ttatattttg atgctcgtta agaaatacca tgattcacac tgcaaggatc
     3421 atgagattat catcgctatt caaaaagcta tggatgcatc tcctgaatta agaagcaaac
     3481 gagatctcat taatcacttt ttaactggaa ttaatgaaat tgacgatatt attgccgctt
     3541 ggcacgacta catctcagaa cagaaagaaa aagaaatcaa acgcattgtt gaagaagaag
     3601 ggctgaaaaa cgataataca agagaattca tttccaattg tctaaaagat ggctatgtaa
     3661 aaactatcgg tacaaaaatc gatgagataa tgccgccaat gtcaagattc gaaggaaaac
     3721 acagggcgga gaaaaaaact cgagtcataa atctgataat tacttttttt gatcgcttct
     3781 tcggaattgg tgaataaatg tattttgctt gaaagaatat tatgcaataa atgttttcac
     3841 attaaagagg ataattcttt gttttattca ttcttgaaga aaggaaaacg tggtggaaaa
     3901 aaatgtcata gtcaatgcgc tttacgaaca gtatgcaaaa gtagttaaag aaaaaagagt
     3961 agcgcaacca gaactcgaat tttctttcat atctggaagt attgaaagct gcggatctga
     4021 caactgcttt atatcggaac atccggaaag cgtctatggc tttgcggtat atgtagaaga
     4081 tcacgagaaa gaatcgatgt ataagaaatt aattgaaaca aatgtaggcg ttagagataa
     4141 acgagaatat cgactcgcaa acgctaacag tgtgaatgag tggtcgccgc ttgcaaaaag
     4201 caaatattat cctttgtact ggggaaaaag ccaatgcctt ttcaaaagaa taattgatca
     4261 tacaaagtcg gcagtaggag taggatctct ttggcttcat agaaaccaag ctctacatgg
     4321 taaaaaagta atttttggaa caatcccgtg ttattttcca aaagaagtcg aggaatcatt
     4381 acaaaaagca tacaagcagg ttcttaagac gcatagagaa ccttaaaaat ccaaataatt
     4441 tttgtatttt aaaaatatac agaacatcct atctgtggct aattgtgaat ggctagttgc
     4501 taattgcact aatgtttaaa ccttaggcca tctttatagt gtaaatttga tacaatcatc
     4561 aaccgttctg tcaaccgata tcctagagtg gtttttaatt ttacatcaat ctaatcattc
     4621 taacgcaata gcatttgtaa tttaaaccaa ctcatttgtt gttatataat aaagatacga
     4681 aaattatcaa ccatgtgtga ggaataatca tgcaagaaat cttaatctac ctcgaaaagg
     4741 aattcgccga ttaggaaatc agccacattg gctttcaagt caaatcatca ataaatacga
     4801 tattaagata gtgtcaaaga caaaagatcc gcttatctcg atgtatggct tccacgtcat
     4861 acctgactac acagcagacg gaatacaaaa tgataatatt agcaattatc ctatgctcat
     4921 cctcagtggt ggaatgtaat ggagcataga cagtcatggt tccaacagcc gagccgcgtg
     4981 gacacagaac atgccatata ggtgacccaa aaggaaacct catcgaattt ggctctttta
     5041 ataagcccat tgcccgctga gtttgttata ataatttgaa gatatgcagg ctaacgataa
     5101 agtcgatcaa atcactccca aaataagcac tttaacaatc gtgctcgctt ttttatcttt
     5161 tttatttgtc gttttagctt gtctacttat gtatttctat catatggcgg tttggacatt
     5221 gattgtgaac tttcaaatta tctattttgt gtgggttctt atatttgccc ttgctgtcgg
     5281 ccttttgatt atcatcttaa gcggtgtcac gatgcgaaaa gagaaaaatg gcaacaaact
     5341 cgttcttctt ccgccttttc ttgtcgtcgg tttagctatc ttttcaatgt cgtttggtct
     5401 ttttgaaaaa tttgcgtacg aaagacacta taccttcagc gtcgaaaaat gggcggtggc
     5461 ctctagtgac gaaagaagcg tctatctcga ctcttttcta gaacaatacg atttatatac
     5521 ttttaatgat cagatgatcg ttgccactct tgacgagcct gatgaaaaac gcacgattga
     5581 actgcaaact gatcccgttc aatttggcgg ttatagttat gtttatgatt taggctttgt
     5641 ccgcgattat atggatccga gtttttttga aataaccact gatcaaagcg gcgttgtttc
     5701 ttattatcat atttattcaa cttaacttaa taatttataa tagcgaattc aaactaacgt
     5761 atcatagata ttaatctatg cattgcatct ttatacattg atgatgcctt gctttttata
     5821 tccgcaaacg gtaaatgatc gggatttgtt tcaatgatag tttcgatata aggacacttt
     5881 tcttgtgctt cttttaaaga aatattgctg cttccgacaa tgaggattag tttcttattg
     5941 tttctttggc atctcgtggc gataccctta attgtcttac cataaaaaga ttgctcatcg
     6001 agtcttcctt cacccgagat gacatgatca gcatcttcta ttgcgttatc aaaagcgatg
     6061 agatcgagaa tcgtttcaat accggattta agcgtcccct tagcaaaagc caagatacct
     6121 gccccgatgc ctccggccgc ccccgctcca gggttgcttg cgacatcgat atgcaaatct
     6181 ttcttgatga tggccgctaa gtgtttaaga cctttatcaa gctcgataac catcttgtta
     6241 tccgcgcctt tttgcagggc gtaaacataa gccgcgcctt gtggtccgtg cataggatta
     6301 tcgacatcgc acatgccgat gatttcgatt ccctctaatc gtttatctac aaatgaatta
     6361 tcaattctga cgatatcttt aagcgtgctg cctacaggga tgaattcttt atttctgcca
     6421 ttgtagaact tggttcccaa agccgcgaag agaccgcacc ctccgtcatt cgtggcgctt
     6481 ccgccgagga agatatagat tttcttgccg cctttattga tagcgtctaa tacttgctga
     6541 ccaagaccaa aagtggtggt cttcgctgga tttaaatcct cgattaaagt tagcccggca
     6601 gtagaggcgg tttctaaata cgcgacattg ttatcatcaa gaaaatagcg agagtcaata
     6661 tcgacaaaat agggattctt ggctgttaaa tttatatatc ggcctttaag cgaaaaagcg
     6721 atggcctcaa gcgagccctc gccgccatcg gcgatgggaa gataggcagc cttaattaat
     6781 ggatacttga ctaattcatc tttaaaaaga tggcaaatat cgagcgacga taaggttcct
     6841 ttaaaggaat ccgaggcgat cacgattttc atcattccta ttttacctac tttttatcaa
     6901 cttttacaaa ttaagaagtg ttatgtatat aatagagata tcaataatta attaggggaa
     6961 aactatgcgc acagttaaag cttttttcgc cgagattgcc aacttcatcg taagagccaa
     7021 aaaaccatta atcgttttag gctctttttt agccgccacc tttttatgcg tcggcctcgc
     7081 tgacggcatt caaaaggatt ggggtaacat tcaagtaaca agcggaatga tcaatgccac
     7141 gaccgtcgat aacgaggact attcgatggg ctataaactc tatgtcccaa agagcgccac
     7201 acaaggcagt ccggcgccgg ccgtcttgtg tttgcacggc tatcaaaacg accacgagac
     7261 gagcgctggt tatgccctcg aaatcgcccg ccacggctat gtcgctttag cgattgacga
     7321 attcggtcac ggtgcgacga caattggcat cgccgctcgc ggctgggtta atcataaggt
     7381 ttctaatgtc aactatggct gggagtcgga agcggataag acatattctt ttatcagcgg
     7441 tcccgaccgc tacaaagtga tgatgaactt ctccaacatc gacttcttca tcgataagta
     7501 ttcgaaagca atcgatcccg atactggaga tgttatcgtt gacgaaaact atatcaaaga
     7561 cgcctcgatg ggcggaatcg ccgcctatac gtggctggcg acgcagaact tcgtcgataa
     7621 gaccaagatg gctgtcaccg gccattcgat gggcacgtgg gcggcctggt cggtttcggc
     7681 ggctatgtgt gacgcgcaga tagagggtgt tgatgttacc cctagggcga cgattctcca
     7741 agccggcgaa atctttaaga cgacgcgcga tggcgatggc cgcatggctt acgatagcga
     7801 tgaagatagc gtcgccgata tatattggaa taatccgtta ttgctgtccg ccaaatacga
     7861 tgagtttaat atgtttcgcg attatcaatc gccgcctaat ggcgacttag tcatctccgg
     7921 cgaaccatca agaaattttc ttaatccggg taccggagaa gaaccggaat ggaataaaac
     7981 attccacgaa gactttgccc tcggcacatc ggtcagaaga aatctttaca ataccaacca
     8041 tcgcttgctg acccatcatc gggcggcgat cgccgatact cttgagtggt taagcctttc
     8101 tagcggtgtg tctttatcaa tagataaaac taatcaaacc ttcttagtca aagaagtatt
     8161 ggtctttgtt gccatgattc tggccatggc ctccgccgtg acnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnggcaagtc cgccatcgtc gtctttgccg gcgttatatg
     8341 gatgtatctc caagtcgtta tttcacaagc ccttttccaa cttgatttcc gctttatctg
     8401 gcccttcttt aaggccttta acggcgcgcg attcttacag ttcctcgtct atcttccgat
     8461 ctttgccatc ttctttattt taaataactc caagatcttt gccggcaacc gcttgcccgg
     8521 tacgaatgag acgggatttc tttctttcat caaggtgtgg tggaaatacg ccctttgcat
     8581 ggtcggaggt attctcttta ttattctttt agaatacatt ccgttctttg ccggcatcgg
     8641 cccgggcgcc gatcttgtct tcagcacgac cttcggcgga ccattcatgt ccttattaat
     8701 cgtctttgcc ccgcaagtaa tcgtcttctc gcttatttgc acatactgct ataaaaagac
     8761 cggcaatgtc tttgtcggag cgatgctcgt cgcgattctg gcctgctgga tcatcaccgg
     8821 cggaagcgcg atgatgtatt ccttctgata tcatatgaac ttaataataa aaggcgtcta
     8881 ttccgcgaat cggaaaggac gccttttttg tttggttatg actatttttt gctttgctcg
     8941 ttaatgtaag ccgctaaacc cttctttcgg aaatgatacc aagtatcgat ttcctgtgct
     9001 ggttttgcct cgagcctttt gacaatttcg cggacaaatt cgtattcgcc agtgccgttc
     9061 gcggtgatga agctttcgct ggcgacacat tgctcattga tataatgact atggcctttg
     9121 tatgacgggc agacttggat gatgtgaccg atggaatttc cggtgtgcgg agtgtcatca
     9181 agatatccgt gacacgccaa gaaggcggtc gcgtcacaga tggcggcgac cggtttgtta
     9241 ttctttacga ataaatcgac gaggttcttc gcttcttcat taataaaatt ggctttgctc
     9301 caatacatgc ctccgcacag aataagcatc gcgaacttgc caatttctga attcagcact
     9361 tcatctgccg cgtagtcggg aatcatgcat aaactcgaca tcgagacaac cgggtctttg
     9421 gtcttagcga catatttaac taaatacttg tttgatgatg tgatttgcga accgatatgg
     9481 cttatttccc agtcggcgta accattttca agatagataa atacttcctt cataagcact
     9541 ctccttcaac gcgttgatcg tcatacctca ttatatactt gattatttag aaaatatgca
     9601 accttcgaaa taagaaatca aaaaactgca catagtgcag ttcagcaaaa ccaaggttct
     9661 caattagtta ttcggttggc accttgcgaa tcttgcgaaa gacaaaccag aaaagggcca
     9721 atatttcgat gggcaccaag gcaataaaga tataccagat atgcgggtaa aatttaaaaa
     9781 gtgatagata gatcgagaga gttaacccga cagaaagggc tgttacaaaa gcgaaagata
     9841 ggcggttatt gccccacaat agagaaaaga caagaaggac aattgaagaa aggggaatgg
     9901 cgtagataaa tactaaccag atgtcgaagt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn ncccatttgt ttacggtaaa aggcgatgtt gtcagcgaca attcttttga
    10081 attcggtttc attcataatt cgtattctat ctttttttta ggaaaaaaga aacgatttaa
    10141 tctcgccttc ttctccgagt agagtggtga tttggcattc tactgaggcg cgtccaagtg
    10201 tttactgaca tctgcggtgg attggttata gtgaagtggc aagtaaaaac aaaggagcct
    10261 tctatgaaaa ttgccatctc aaccgaaacg acggtcgact taaccaaaga cctcttaaaa
    10321 gaattcgata tccacaccgt tgcctttacc atcgtcatgg gcgaagaaca agtcgtggac
    10381 ggcgaaataa gccctgagga aattttcagc tttcacgaga gaaccaagat tcttccccgg
    10441 acgagcgccg tcaacgaata tcagtatgaa acccactttc aggagttgct caagtcctac
    10501 gatgccatca ttcacatctc cctatcaagc acattaagcg tggcttacac aaatgcctcg
    10561 aatgtcgcca aaaaactaaa caatgtcacc gtcatcgact cgcgcagcct ttcgacgggc
    10621 atcgccttgc tctgccttca tgctcagaag ctcgtcagag ccggcgaaag gctttctaac
    10681 atcatcaact ccatctatga acggattccc tttgtccagg ccagctttgc ccttgaaaag
    10741 gtcgattatc tttataaagg cggccgctgc tcactcttat cgtttatcgg cgccaatctc
    10801 ctcggcatca aaccgcagat catcgtcaaa gacggcaaga tgattcccgg acgcaaatat
    10861 cgcggcaaga tgattcgcgt cgtcaagcaa tacgtcgaag atactttgca gtattatcat
    10921 aatcccgatc tagaacaagt cttcatcacc tatacgaccg cggacttcga aattatcgag
    10981 atggtcaaga caatgcttgt tgaacgtggt ttcaaacgca ttgaagtgac aagagccggc
    11041 gggacgatct cctcgcactg cggcgagaac tgcctcggca tcctctacat caacgacggc
    11101 gatcgttaat ttacattaac aagacataaa aaagcacctt ttacgaggtg ctttttatct
    11161 atgataaagg tgacttattt gctgggggcg aaagccatca agccttcgag gacgccggcg
    11221 gataagagaa gaacgctcat accgatgacc gaaccgccga gtttgacact tgcggggtag
    11281 ttaggattaa cttgtccgac taaggttggc atcaataaga agatgatgcc ggccacgacg
    11341 aagaatacgg cgatcgcgcc aagaataatt ctttgaaact taaagggaat gaaggccgga
    11401 atgacgagca aggagccgac cgctaagagc aagaaggcga cgatgacgac gaccgaggcg
    11461 acattagagc catgacccca gacgacttgg tagaagttgc cgatattgct ggcgacgccg
    11521 gtgccggtat agtttggcac atagagggcg acggcggcgc ccaagggcca gacgatgccc
    11581 agtaaggcga ggacgagaat gttgacgaag ggaattagtt ttttcatggt gtggactccg
    11641 tttctagaaa atacaaataa attgtatgcg aattaatact atacaaacaa gcgcgaatta
    11701 tcaatagtca gttattttta agaaataaaa gttgtcgctg atgaatgcct gtttagtaaa
    11761 tgtcgcttta ataaaattat tgttgtactt ataatactta ttgtacgaat gaggaacagc
    11821 tatgaagaaa gtttataata cactcgcttc gctattaagc gttttggcgt tggcatcatg
    11881 cgccggctcc cccgaggcgc tgacatatga gcactataaa aagagagatt tagattatca
    11941 ggaaaatttt cgcgttctgc aaatgaccga cattcacttc ggattggcga ccaacttcgc
    12001 cgaggagtgg gtctattttg atgctttgat cggattggcc gagcccgact tgatggtcct
    12061 gaccggcgac atgtttatga acgcttccgt cgatgtcgtc aatcagttat ataactacat
    12121 ggacagcaaa gacattccct ggaccgttac ctttggtaat catgaccgcc agggctttta
    12181 cacgccgcag tttatcgaag ggcaagccac ctacgatagt tacgataatt gtctctatgt
    12241 caatcccgac gacaacgtta acggacatgg caatcactat attaatatcg tctccggaac
    12301 cgatgtcgaa tggcaggtca tcatgctcga ctccggatct tatcattcgc tgggagcaac
    12361 atatgactat gacgttattc acgaagacca aatcgcctgg tacgaggacg tcatcaaaga
    12421 gaccaacaaa gttcagtttg acgtcgatga cttcgccttg gtgccgcccg cgaatgtcat
    12481 tccatccgtc gctttctttc acattcctct cttcgagtat gtggatgcct atgaagcctg
    12541 ggaggcctcg ggttttgacg aagacatggg cagcggcgtc tatcaggatt ccggcatctg
    12601 gcacggctat tataattccg gtttctttgc taaagcaaaa gaacttggtt caacacgtgg
    12661 aatgttcgtc ggacatgacc acacgaataa ctttgcaatc gaatatcaag atatcgtctt
    12721 aagctacggc gttaagacgg gccgcggcat ctatcacgat cccgagatga tcggcggaca
    12781 agtcatcaca ttaggcgatg acggaacgat tgatatcgag cgccttttcg tcgcttacgg
    12841 agaataagaa tatgaaaacg aaaaccctct ttcttgtcac taatctcctt ctcttcttct
    12901 ttgccggcat cagcaccttc ttctttgtca tggtcctttt tacggatgcg attaataatt
    12961 tccgcatggt tctggcctat cttcctttat atctaacttt gattcttccg atttatgccc
    13021 tgatagccgt caatcgcatc gccaaagtcg aaagcgataa tctcaaacta agaaaaatca
    13081 agattaactc gctcgttttc ggaagtatca gcgcttttgc cctcatctat ggcttcatca
    13141 atgtcttcgt ctccttcggc gggaatttcc tttccggcgg accctcaaaa tactttcctc
    13201 tgtcgttttt agtattggat tttttgtttg ctttaatcgc cgtgtatatc ctctttattt
    13261 ttcgctccga gaaatgcgaa aaggaagcgg cgcctcaaaa aggcgctctc aagaatgtcg
    13321 gtggggttct taaaacaatt tttctcctct ttgccctcta tttcttgggt actttcgctt
    13381 ttggttttta tagttttgat ttaagccttg agcatgtcgg cgggacgctt cccgtgtatt
    13441 tgctgatgat tcttccgtct ttgatgattg tcgctaacga gatcgttaaa ggatatgtcc
    13501 ttttcgaaaa gcagaaattc gcccgcttta tcctcggact cagcggttta ggcctcactc
    13561 ttgtcctcgg cgtctggatg tttatctata tgaatgccaa gccgaacttt gtcgtcgacg
    13621 ccctgacggc gagctttccc cttgattttg ccatctcgat taatctcgga ccgattctcg
    13681 tcttaatcct cagtttaatt ccgggactat atttgacaat tgcttacctc cttcccaatt
    13741 attgcccgat ttataagcgc tggagggcca aaaacgacaa tcctacaaag taagcgtgta
    13801 aatatcttgc attaattcgc aactcgtttt attattaaca ccgcgaacag aatcgtgttc
    13861 gcttccgggt tctcccggga caactcttga actaaagagt ggccgcaaga ccactctttg
    13921 tcttcttaaa ggaggtacct atggccgtta aagatgtcgt cagcgctctc atcgaaccgc
    13981 agctgaagat tctcggttat gatctagcaa gcgtctcttt ccgtaaggaa ggcgcgaatt
    14041 acatcctgcg cgtggtcatc gataaagatg ggcccgtgag catggatgac attgtgcgcg
    14101 ccggcgattt aatcagcccc ctgctcgacg aagccgatcc gattaagaca tcgtatatgc
    14161 tcgatgtcac ctcgttcgga gccgaaaagc cgatcaaact cgaaagattg gaacgctatc
    14221 tcaacgagcg cgtcaatctc ggccttcgcg aagccgtcga caaaaagaat gccctcgaag
    14281 gcgaactcgt cagcctcaca aaagaagaag taacaattaa agtagacaac aaaaagaaag
    14341 gaaacgtgac tatccgccgc gacttgattg ccaatgcgcg cctagccatc aaattttagg
    14401 agaaagacac tatgattaac gcagcacagt tttttgaagc cctcgaccag ttggaagccc
    14461 agaaaggcat ttcgaaagaa agcattttgg tcgctttgaa ggaagcgatg aaaaaagccc
    14521 tggtcaaaca actcggcggc ggtgacgatg ccgtcatcga cgtcatcatc gacgaagaga
    14581 ccgccaagat cgagatgtat gtcatcaaga aagtcgtcgc cgaagtcgct gacgactatc
    14641 tcgaaatcag cctcgacgaa gcgcgcaagt tcgatcaaaa atataatctc ggcgacgatt
    14701 ataaaattta tatcaatatt cacgacttga gcaagctctt tgccatgacg gtcaaatccg
    14761 tcctccatca aaagctcgcc gaagccgaga aagccgctct ttatgtcacc tataaggata
    14821 agatcggcga gatgattacc ggcgtcgtcg aaaaagccga tgagcgctcg attatcgtca
    14881 acatcggccg cacctcggtt tatcttagca atcgcgaact gatcggcgaa gaaaccttta
    14941 atgtcggcga taccatccgc ttatacgtct ccgatgtctc gtcgacggag aaaggcgcgc
    15001 agatcgttgt cacgaggagc gatcccggct tcttaagacg catcttcgaa gaagaaattc
    15061 atgaaatcta tgacgggacc gtcatcatca aagatatcgc ccgcgaagcc ggcacgcgca
    15121 gtaaagtcgc cgtcttcagc catgacccca acgtcgatcc gaccggcgcc tgcatcggcc
    15181 acgatggcgg tcgcgtcaaa aagattctcg gccaattagg caatgcccgc gataaagaaa
    15241 aaatcgatat catccagtat caggaggatc cggctcttta tatcgtcgaa gccctgcgcc
    15301 cggcgacggt cgtcggcgtt cacctcgata aggaagaaca tgccgcttta gccgtcgtca
    15361 aagacgaaca gctctcttta gcgatcggcc gtaaaggggc caacgcccgc ctcgccaacc
    15421 gcttaaccgg ctggaagatt gacattaaag aagaagcgat ggccaaagaa gctaatctcg
    15481 aatacttcaa tgtcgaagaa ctccatcaaa gacacgccga tcagcttaaa gctgaggctt
    15541 atcagcgcta catcgattcg attaagccga cacctaagga tgtcgtcgtc gaaactcctg
    15601 tggctagtga acctgccgcc gctcccgtca gcgatgtgac gccgttagtc gaagccaagc
    15661 cggtcgtcga agaagccccg aaagtcgtcg aagagccgat taagattaaa atcacgaccg
    15721 atctcgagac gctcgaacgc gaactcgaaa gtcaaaaaga gaagaataaa caagccgctc
    15781 cgaaggccaa aagaccgcgc aagatttccg atgacgaagt caagagcgaa gccaaggacg
    15841 aaaagcctta cgtcgctccc gaagccgaca ataaacccta catgcaaatc tattccgaag
    15901 aagaactcgc cgagctcgaa aaggaagctg ccaagcatcc cgatgagctt atcgatgaag
    15961 aagagatcga ctacgacgaa tacgatgcct attatgagga tgaggaataa tctcattgcc
    16021 ggatagccag gaaaggaagt gatgcgaatg aaaaaaattc cgctgcgccg ctgtttagcg
    16081 agcaatcaga tgtttcccaa gaatgagctc cttcgcatcg tcaaaacccc ggccggagaa
    16141 atcgtcatcg accgcaccgg caaagttaat ggtcatggcg cttatctaag aaaagatgcc
    16201 gagattttcg ctttagcgaa gaagcggcgc ctgctcgata aagcctttgg catggccgtc
    16261 tctgacgagg tttacgattc cctcagtaac tacttatgaa taatcgtgtc agcggttatt
    16321 taggactagc caatcgcgcc ggcaaagctc tggttggcga agccatcacg aaagtctggt
    16381 ccaagcggaa gatctatctg ctgatcatgg ctttagatgc tgtgagcaaa acgcaggaaa
    16441 aacttgccaa actagctcgc gagcaaaacg tcacgaccat ctatgcctat cgcaaggccg
    16501 aactcggcca tgccttaggc tttgcggaag tctcgaccgt cgccatcagc gacaaaggac
    16561 tttccgtcca actcattagt gtcattaagg aggataacaa atgaacgata agaataaaaa
    16621 gcgcaaccgc gtggccaatg tgcccgccgg aaacacaccc cgcaaaggtg aaaatgccaa
    16681 ggtcaacaag ggcgttttcg tttatacgaa atgcatgtcc gtcggcgaac tggctaagca
    16741 actggaaatc aattcctccg atatcatcaa gtacttgttt atgaatggca aagtcatcac
    16801 gatcaaccag accctcgatg atgaagcgat cggcaccgtg tgccttcagt ttggctttga
    16861 tttccgcaaa gataaggaag tcagcgaaga aaactttgaa gaactcgaaa tcgtcgatga
    16921 ccccaaagat ttaaaatcgc gcccgccggt cgtcaccatc atgggccacg tcgaccacgg
    16981 caaaacaacg ttgattgatg cgattcgctc ctcacatgtc gtcgaaactg aagtcggtgg
    17041 catctcgcaa gccatcggcg cctatcaaaa agaaatcaaa ggccagaaga tcacctttat
    17101 cgacactccc gggcacgaag cctttacggc gatgcgctcg agaggcgcct cgattaccga
    17161 tatcgtcgtc ttggtcgtcg ccgccgatga cggcgtcatg ccgcaaaccg tcgaagcgat
    17221 taatcacgcc cgggctgcca aggtgccgat tattgtcgct atcaataaaa ttgataagcc
    17281 gggtgccgac gccaagcgcg tccgcaatga gctcgtcgct tacgacatca ttcccgaaga
    17341 ctacggcgga aataacatct tcgtcgaaat ctcggccaaa aaaggcatcg gcatcgatga
    17401 cctcctcgaa aacattatac tcgtcgccga gatgcaggaa ctgaaggcca accctcgtcg
    17461 ctatgccctt ggcaccgtca tcgaagccaa cctcgataaa ggcgaagggc cgaaggcgac
    17521 tttactcgtc caaaacggca cgttgaactt tcaggattac gtcgtcctcg gttcggccta
    17581 tggcaaaatt cgccgcatga cgaatgaata taagaaaatc ttgaaggccg cgggaccttc
    17641 gacgccggtc agcatcatcg gtttgagcga agtgcccaac gccggcgatt cgttcatggc
    17701 ttttccaagc gaaaaacaag cgcgcgacat cgccagcaaa cgccgcctcg ccaaagaatc
    17761 agaaggccgc gggggcagtt cagccgcctc gcttgacgat ttatacaacc gcattcacga
    17821 aggcgaagtc gaaaacatca acgtcatcat caaagccgac acgaccggct cggccgaagc
    17881 cattaaaggg gctttggaaa aacttgagac cgaaggtgtc aaagttaata tcatcgcctc
    17941 gacggccgga gccatcactg aaagtgatat tctcctcgcc agcgcctcgc acgccatcgt
    18001 ctacggcttt aatgtccggc cgaatgcttt agtcaaagcc aaggccgatg aagaaagcgt
    18061 cgagattcgc ctacatcgca ttatctatgc cctcatcgag gagatggaag cggccatgcg
    18121 cggcaagctc aaggtgcaga tggtcgaaaa gattaccggc gcggccgaag tgcgcgtcat
    18181 ctataaagtt tcaaagctcg ggacgatcgc cggctcctat gtcactaatg gcacgcttaa
    18241 acacggtcag agcgtccgcc tgattcgcga tggcgtcgtc gtctacgaag gcaaaatcgc
    18301 ttcgatgaag cgctataaag acgatatcaa agaagcccgc gaaggatatg aatgcggcat
    18361 cgtcttagat ggctttaacg acgaaaaaga acaagacatc gtcgaaggct atgaaatggt
    18421 ggagaagaaa taatttatgg ctaatcgcac ggaaagaatt caaagcatca tcgccaagaa
    18481 catctcggaa attttgcaat ttgaagtcaa gaacaaaagc gtcggtctcg tctcggtgac
    18541 cgaagttcgc gtttccagtg acttttccta tgcgaaagtg tatgttagtt ttatcggtgc
    18601 caagcacccg aacgtcaacc tcgaagcgct caatgcggcg aaaggattcg tccgcagctc
    18661 gctggcctcg aaacttgata ttcgcaaggt tcccgagatc tcttttattc tcgatgatac
    18721 cttcgaaaag cgcgatcgca tcgaaaaaat cttgaaagaa gaagctgaaa cgctaaataa
    18781 actcaggggg aaatgatatg agcaacggaa gcgacatcgt cgtcgagacg aagggtctta
    18841 ccaagaagta tgggacgacg ctggctctta acaatgtcag catcagcgtc caccgcggcg
    18901 aaatctatgg cttcgtcggt cgtaacggcg ccggaaagac gacgctgatt cgcgtcctga
    18961 ccggtatcgc caatccgaca tccggctcgt ttacgctttt aggagcggcc actaaaaacg
    19021 agcagaaaga agcgcgcaag cacatcgcgg cgatggtcga aacgccggct ctttacatga
    19081 atatgacggc gcatgataat ttgcgcacta cccttatctt aaaaggtctc aaagacgaat
    19141 ctttgatccg ctcgcggctt gaagaagtcg gtttgagcga tgttatcgcc tcgagcaaaa
    19201 aagccaagga cttttctttg ggcatgaagc agcgcttagg catcgccatg gccttgctcg
    19261 gcgaccccga actgctcatc ctcgatgaac cgaccaatgg ccttgatccc gaaggcattc
    19321 gagaaatgcg cgaactgctt gttaacatta ataagagccg caatatcacg atgatcatat
    19381 caagccacat tctcggcgag ctctctaaac tggcgacgcg ctttggcttt attgacaaag
    19441 gcgtgatcat caacgaaatc tcttccgatg atctatttaa caaagtgagc aagtcagtca
    19501 tcatcaagac cgatgacaat gtccgggctc tcgcgatttt aaacggagcg tatcaaaatc
    19561 gcgtgacgag cgaaaacggc tcaattcatg tcgccgatgt caataactcg agtgaaatcg
    19621 tcaaactcct cgtcaaatcg gatatcaacg tagttcagtt ccgcgaacag gaaaacggcc
    19681 ttgaagatta cttcatcaat ctgatcggag gaaatcacca tgactaaatt gcttaaactc
    19741 cagttattcc gtgcgcttaa agataaggga acgattgtct ttacgatcat caccctcgtc
    19801 ttaggcatcc tctccatcac ttttaatttc ttgatctccg atagcatcgg tcaaggcggt
    19861 gcgaccctga tcggtgatct ttttgcgatt tcatcattga tcaattcatc atattcgcta
    19921 tcgagcacgc cgatggtctt actcattatc atgggcgtcg tcttgcttta caaggaatcg
    19981 ggttggggaa caattcgcaa tcaggtggtg gccggctatt cgaagattca aatctacttt
    20041 gctaacttct tagctatgct aatcattttg tttaccatct tgctaactta tcagcttctg
    20101 acgttaggac tcggctttat cttcggcttt gagctcggca tcgagacggc cgcggaagtc
    20161 ggcatcttct ttcgcaagta tgctttaaac ctcgtcattt tcatcaccga agtggcgttt
    20221 atgaccttta tctcattgat gtttcaaaac atcatcgccc ctgttttctt agtcgtcatc
    20281 ctgccgattt ttgtcggagc catcctccag acggtcgtgc cccttgtcgt catcttaaat
    20341 caggataacg ccgcccagat catcggcggt tttgagtatg tcatctggta tcagaatacg
    20401 tctttgagca tcagcggctt tacctatgaa ctgacgagtg gcgtcggaag cctgatgggc
    20461 gcgacgcttt atgacacaag caccttagga tttatcctaa aaacaatcgg gacgaatctt
    20521 ggtctcaacg gtattttcct tggcttaggc agttatctct tctccagaaa agacctgaaa
    20581 taaatatctt atttgtcgcg ttttaaaacc atcttaaagt gggggatgtt cgcgtctaaa
    20641 tagacgtctc ccactttttt aaagcccagc ttctcataaa agccgagggc ctgaacctgc
    20701 gctcctaaag taaaaatcag gggattggtc ttggccaaga gcatttcgat cagaaaagag
    20761 agggctttct tggtatagcc aaagccccgg tattgtttca gtgtgcagat gcgcgagaga
    20821 tggatgagac gctcttcgtc aaaggtatag cgaatcgctc cgatcggcgt ttgatcttta
    20881 tagattaaaa gatgttcctg accctcgtcg cttccgtcga actcgtcttg cggcggaata
    20941 ctttgctcga ttaaaaagac atcggtgcgg atcttggcca taatcagggc atcttccttg
    21001 ctcagaactg gtttcagcga taaatcgcta ccttcaatct tataaaagat ttctttaccc
    21061 cataagtctt cttgctcgta gttataaagc atcgttaaga agttctcttt ggcgtcggga
    21121 tattttttgt atattccagc caacagattc ttgacatcct cgcgagtggt gaagccatcg
    21181 ctcgggcatg ggctcttcat gaccggaaac ttttcttcgc gcgctgtttt ctcgatctca
    21241 ctttcacggc ataagatgag tggccggatg aaatcgatat cagctttttg taggtgcatt
    21301 ttgggtgcaa aagtcgctat tcttccgccg tagagctcat tcatgaataa ggtttcaatc
    21361 gcatcttcgg catgatgggc aaaagcgact ttcttcgccc ccagcccttt ggcgacttta
    21421 ttgatggcgg ccttcttcat ccgcgagcag atcgaacagg ggagatgtcc gccttctttg
    21481 gtgtgtttgg caaggatggg atagacggta ctggcatcgg cgatgactag ttccatacct
    21541 agtgttctaa catactcctg aatggcggac ggatcaaaac tagcaaagcc gagatcgagc
    21601 atgacgggta tgatttcaaa gtcgcactgg gcgaatttac gatatagatt caacgcgtat
    21661 aaaaggacaa gcgaatcctt gcctccggag acgccgacga caatcttatc gccctgattg
    21721 attagcgaaa aacgcaggtc ggcattacgg atggaagcta agacttttct aatcgcgcgc
    21781 atttgttata cccaagatat caagttagat tataatgttt accatggaaa gtgtgaatca
    21841 aaaaagcgga atccttttgc tcgataaagt aatcgggccg acctctcggc aagtagtaaa
    21901 cggcgtcact tcccttttac gcaccaaaaa aatcggccac gtcggcacgc ttgatccgtt
    21961 tgcttccgga cttttgattc tgaccgtcaa ccaggcgacc aaaatcggcg cctatctcga
    22021 ggctctcgat aagacatata tcgcgcattt aaaactcggg attcagaccg acacgggtga
    22081 tttgacgggc gagacaatcg ccactcaaaa agtgccaaat aaccttgatt ttgcgataat
    22141 aaatgacgtt ttacactctt ttttaggaaa aagactgcaa atccccccga tgtacagcgc
    22201 cctcaaacgc gagggaaaac cactctatag atacgctcgc gaaggcttag aagtcgaacg
    22261 ccagccacgc gaaatcgaaa tctttgacat cgagctatta agttttgaaa atgatgtcat
    22321 cgaattctcc gctcgcgtca gcaaaggcac ttacatccga actctcggtg aagaaatagc
    22381 ggtggccctt catactctcg gacaccttat ttccctccgc cgtaaggcga ttggcaactt
    22441 tcgagttgaa gatgccgagc ctcttaaaaa agtcgctttc gaaaggctta tttcgattcg
    22501 caaggctctc tctcatcttc ctgtgtttga ggtcgatgaa gcaagcataa aagacatcat
    22561 gaacggcaag ccagtcaaac tcccggaaat cggccagata gtccttttgg tgagcgacga
    22621 aaaagcattg gccttatacg aaaaacgcga tgatggttta tactattctc gacggggact
    22681 gttttaaatg aaaacgattc tgctcgagct tgaaaatctt ccgcgctttg cctcgccgat
    22741 cagcatctgc ctcggtaatt ttgacggcgt ccacatcggc catcagcaag tgattaaaaa
    22801 agctgttctc gacagcggta cggaagtcgc ggtcctgact tttgatgctc ctcttggaag
    22861 cgtcatcgac ggaagaaaag ctaagcaggt tttgacctcg gtaagcgacc gctttcaaat
    22921 tattcaccgc ctcggcgcgg actatctttt cgtccttcat ctcgacgaaa aattgcttag
    22981 catgtccgcc gaggatttta tcgtcaaagt cttgaagaaa ctcaatgtcg caaaggtttt
    23041 tgccggccag gattatcgct tcggccacga gcaagccggc gacatcgaat tgctcagcaa
    23101 acacttctcc gtcacgagcc ttcccctttt tgatgtcgaa caccataaag tatcgacgag
    23161 tcagatcatc gctttcctca agcagggcaa gattaataaa gccaatgaac tcttgggccg
    23221 ccactatcag atgcacggca cgatcagcga aggactgcac aatggcaaga cgatcggctt
    23281 tccgaccgcg aatttaaaat taagcgcccc ttatgtcatt cctaaattcg gcgtctacaa
    23341 aacgatttgc tatatcgatg gctttcctca tctctcgctt actaatgtgg gattacatcc
    23401 gacgattcag cagctcgaca tgccttcgat cgaagtccat attcctaatt atgaagggga
    23461 agattacggc aagacgatgt acttggaatt catcgctttc ttaaggcccg agaggcgctt
    23521 taccacactt gacgaattaa aggcacaaat ttcccgtgat atcgtctcga ttaagcccta
    23581 gaagaaataa taattgcatt ccgttttcaa taactatata tttatataag cgacttggtc
    23641 gatgcctggc gagtctccga cgctaagcgc tgatcaagct aagtaccaag aaggaggaaa
    23701 tagacatggc tctatccaaa gaaagagtgg ctgaactgat taaagaatac ggcaaaaatg
    23761 ccaatgattc cggttcaccc gccgtccaga ttgccctctt gaccgagcag attacaaatc
    23821 tgaccgctca tctcaaggcc aataaaaagg accatcactc gcgccgcggc ttattgatcg
    23881 ccgtcggcaa gcgcagaagt ctgctcgatt a
//
LOCUS       DAOSSL010000015        23166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2384_length_23166_cov_16.444637, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000015 DAOSSL010000000
VERSION     DAOSSL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 23166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23166
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2384_length_23166_cov_16.444637"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1270
                     /locus_tag="PLH17_02555"
     CDS             77..1270
                     /locus_tag="PLH17_02555"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOR20692.1"
                     /translation="MVKILVVNAGSSSLKYKLYEMPEEEVICSGIAERIGHENAIFRL
                     KFNGDSIKTILPIKDHTKAVELLLDALIVHRVINNFSDIKAVGHRVVQGGKYFSHSVF
                     FDDDTIDKVESLIELAPLHNGPHLIGVRAFKSILPHIPNVATFDTAFHRSMAEVDAIF
                     PIPYELTQEFDIRRYGAHGTSHQYLDQEGRKLIPDVEHPRIITCHIGSGASIAAIKDG
                     KCVATSMGLTPLGGIMMGTRTGDIDPSVVYFLCKKLNKSIDEVYEILNKKSGLLGVSG
                     VSNDSRDVLKAMENGNPRAVLADALFNRRIADYIGQYFVRLGGADLIIFSAGIGENSA
                     YFRSRVIEEIGPALGVKIDETINESSRGVSVLLSTPDSKVKVALIPTDEEVMIARDTY
                     NLISK"
     assembly_gap    1399..1498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1574..2698
                     /locus_tag="PLH17_02560"
     CDS             1574..2698
                     /locus_tag="PLH17_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20693.1"
                     /translation="MKLKNLLPVTLLAVFALAACNPTDTSSSGDTSSEIEESALKDII
                     AGLPLGEVDEAGLTSIYANLGDVTLGIQQSAFEGMSGIEPRILTDGSQGYMEYISGSG
                     AIDGDVVQVAVETELSLAESDGAGGYVLGEAVPDISEVTTVWKDDTSVYMANVVNEDP
                     ADDYSFLATAPVADVDVALFKDFVNSGDLAYYGALAAEASTAFVVPDDFAEVTLIQKA
                     DKFVDSETDDVYLHLVNYTLGSWFGLYSAEAVGNAAGPGKYIDRGEVWGFEAYINADG
                     VLDSVYTYSDHFINWTYQDLNWEEGDPAPSDPLTPAEIEALDLELLGESWAFVYGVMY
                     HEFSSEAPDAVVVPAVDGLSAADGSDAAYLFDPADYGFTL"
     gene            2830..>3628
                     /locus_tag="PLH17_02565"
     CDS             2830..>3628
                     /locus_tag="PLH17_02565"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOR20694.1"
                     /translation="MPSKIDAIILEEAKAYKREHNRIIKNIIKYDRIAIFRHIMPDFD
                     AMGTQMGLYTWIKDNFPNKEVHFLGDNHVTFTPRLFPETERLNDEWFKEPFLAIIIDT
                     ANTSRIADPRWKKAKYKIKIDHHPDVEDFGKTSIVNTSMAAASEVVVNMLLSVKGDYV
                     MSKQAASYFYIALAGDSGRFQYNSTTPHTFAIAKELVKTGFVLSDIYKKMYFKKIEDL
                     RVTAYVLNNFHVSEHGVAYYVLSSEVQKELNITTERGKENVNLFSNID"
     assembly_gap    3629..3728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3729..3986
                     /locus_tag="PLH17_02570"
     CDS             <3729..3986
                     /locus_tag="PLH17_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOR20695.1"
                     /translation="NITTERGKENVNLFSNIDGINVWCSITEDAKDKCYRVSIRSKEK
                     TINQVAAKYEGGGHAQASGAKLKSLDVLPQFIQDLDNLFVE"
     gene            4069..7113
                     /locus_tag="PLH17_02575"
     CDS             4069..7113
                     /locus_tag="PLH17_02575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOR20696.1"
                     /translation="MSFIPLHVYTGYSFLQSGITEENYLKALKKRGFKGSGITDFNSL
                     SGVPHFYKALKQEKLNLLAGVDFLIDDNLLSFFALNEEGYRNLLKLLFIKRTRNLTYE
                     EVGRHKAGLAVILAIAHSKLSSLFETERDQIPHFLLPISNLGEHFYLGIEFGVEEDEL
                     FVAYMRDFARTHGYQTIAFPFIRYIDKKDAIVLEIAKAIRDDSELAIQELEGSEFLLT
                     KEEIARFFTEQEIENTNRLLKLSSFRFEEKRGHLLRYQKSDDITSDAYLKNKASEGLQ
                     NRIPVIGEEYLSRLNYELDVIAKMGYSDYFLIVSDYVAFAREHGISVGPGRGSAAGSL
                     VSYALGIVSCDPIKHNLLFERFLNPERQSMPDIDIDFSDVRRSEVIDYLRSKYGGDKV
                     ANIMTIQTIGAKQALRDIGRVYKIETREIDMISKLIIDANSSLRENYRKNPAFKKLVD
                     SDKYYLNIVSLAALIEGLPRQSGLHAAGIILNDSPLEEVLPVNVDFNGDYIAEYEMNY
                     LEEQGFLKMDILGLRNLSIIDECLSLIRQNRGISLNYQEIPFEDKKAIRIIKEGRTMG
                     LFQLESAGMKKAISTLEPEVFEDIVALLALFRPGPMDNIPSYARRKKKKEPIVYPSKA
                     LEEILASTYGIIVYQEQITQIVRKMADFSYGQADVFRRAISKKDSAQLSSLEQNFVAG
                     AIKNNYSHSEAKSVFDLIYKFADYGFNRSHSLCYAVLACQMAYLKTYYTPEFYAAILG
                     SSSGSNDTKFNETLNEVRKMGIKILNPDINRSGQTFIVKDGALIFPLFAVKGILGALV
                     DAIIKERNNGGPFTDFFDFVARMIAYKINQNQIGKLIDAGAFDNFAISRSSLRATINS
                     ALRYGSMLYSKDGQMIIDLPFSSKPRYTEAFDDPLDNLDREYEALGLMISGSPLTYKQ
                     DTIKKYPVIAISDARESQSPTMQIVGVIRTVKVINTRSGTPMAFLNIYDDSGDIDITI
                     FAKTYALSYEFLQKKKIVRVIGYMDRKRENVFIANEIIALEE"
     gene            7116..9710
                     /gene="polA"
                     /locus_tag="PLH17_02580"
     CDS             7116..9710
                     /gene="polA"
                     /locus_tag="PLH17_02580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOR20697.1"
                     /translation="MAKVYVIDGNSLLFRAYYATAYGPNAKVMRTKEGVPTNALFAFG
                     NMINKIISSFKGGEHIIVGFDVGKKTFRNGEFEAYKANRKPTDADLVVQMPLARELLT
                     SLGIFVYEQEGYEADDVCGTIAKVASKAGHQVIVYTSDRDFLQLIDHNIAIHILKTGM
                     SNIMVMDEVTMPQEFGISPAQIPDYKGLRGDASDNLPGIPGIGEKTAVKLLQQYGTFE
                     KILEEAQNIDGKIGQNLVEHAKIGQLSKRLATIKTDIELPFTLADTMYQGYVFDTINQ
                     FGQRYELKQLLNRLPSKYKIESERDVKIEYQKISSFKNVELGSKIALFIDMKEDNYFK
                     APILGLALSNGANHYYINLDDAVRDQNLKTMLEDEKISKSCFDIKAATVALHRLGIAL
                     RGVAFDLLLAAYLLDSSLKNNVDSILNYFGVDAHGKDDRELSLFDESNPLRTAKVAYL
                     TLKLQARVIEELKKHEAIKLYEDIEMPLALVLAKMEIEGFPLDAKTLDTIGADYRESL
                     KALTSEIYTLAGEEFNINSPKQVGEILFTKLALPSNRKDSTSVDALKKLIDRHPIVAK
                     ILEYRKYAKLLSTYIDGLVVHIHEDGKLHAIFNQALTTTGRLSSSEPNLQNISIRDEE
                     GRLIRRAFYYPDNNYQILSLDYSQIELRILASLSRCQAFIDVFNRDDDVHAATARAIF
                     GSADSNARRKAKAVNFGIIYGISDWGLAEQLEIPPKEAKGIIDAFFQTYPEVSDYLQN
                     VVDQAFKDGYVATLFGRRRYLRELYDSNYQVREFARRAAMNAPIQGTAADLIKLAMVK
                     VQAMLEKKNYQTRLVLQIHDELLFKVHIDEIDKVRAEISEIMENVIALDVKLKVDGGV
                     GKTWYDAK"
     gene            9723..11120
                     /gene="mutM"
                     /locus_tag="PLH17_02585"
     CDS             9723..11120
                     /gene="mutM"
                     /locus_tag="PLH17_02585"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOR20698.1"
                     /translation="MPELPEVETIKNILAKLVIGKKILSVEIFNRNTIEGDPFDFAKT
                     LKEKTISKLTRIGKFLIFHLDEEIIFLSHLRMEGKYFYHEQMLETYDKHACVVFTFSD
                     HSALEYNDTRKFGLMKLSSAATYQTSPPLSNLGPEPFDIKDVKPLLSKLARKTIPIKA
                     VLLDQTFMAGLGNIYVDEVLYLSLIHPETPADRISESQLKGIISNSVKVLSMAIKSGG
                     STIRSYHPSQGIDGGFQTQLNAYGKENLPCPRCNHGMRKIFVGGRGTTFCPRCQKNPS
                     LPYVIGITGPIGSGKSSVLSFFGERGYHTLSGDTIVKNLYHDKKVQELIIKLFGNNVV
                     DESGNIDKRFIIDVIIADEYKKRGLEKILHPRVEKAVVETLKKAHNDDKIAIEMPLLF
                     EAKLDDYCDETIYVDIEDSIQAKRLAERRLPLATLRALNTGFDALRDKEKATYVVDNS
                     SSLSKLHKQLKRILR"
     gene            complement(11117..12013)
                     /locus_tag="PLH17_02590"
     CDS             complement(11117..12013)
                     /locus_tag="PLH17_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20699.1"
                     /translation="MDKIKVPLFEKTELDNYIDEMIDELRKDVEVYEIIKQTMNPTLK
                     TVRENISKFIAFKEDYDYCRKCPGVDNCRKATPHLQLRLANDDGIIERSFAPCDKILK
                     RIEIDSKYIYADFPEEWKESSLKTLDKTAERNRIIRLFSEIVQGNSHRWVYSRGGHRV
                     GKSFLMATLVNDFIAITKAPAAFINCANRIKEIHDLSFNDKSAFAKKIVELSSLPLLV
                     LDDFGNEYKNDFIRDTIVLPILIERAKNQRLTFFTSDFTINEIAQLYETSKAGAIRAK
                     QLARLLKEMAEEEFDLSGVSAY"
     gene            complement(12019..13272)
                     /locus_tag="PLH17_02595"
     CDS             complement(12019..13272)
                     /locus_tag="PLH17_02595"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOR20700.1"
                     /translation="MDYISEKDYYETRPASLISDIDREVLSELYQPLIGHKSVGIFLS
                     LFHEKMKASEIDTFDHAHLFDKMQMSAGEFFTARRALEAVGLLRTFFKEEGGIRYYIY
                     VLYAPKSPREFFDDVLFKGLLIKYVGEKEAKRLALSYKVDFQTKGFDDITSSFVETFH
                     PDYDSPVFRKDLKVEVKGRKSGRIDTSFDFDAFFSALDKNGQIKREVLTKKDLKEIER
                     LAALFSLDELTMAEIVVDAYDPDNKAQRINFQRVSDRAKEEVKFPFFHRKSKGEKSHV
                     TSDSELASKIKLMDAMAPGEYLRIKQNNTAPSRSDLDIVDDLSKNFGFSHGVINALID
                     YVLAKNKNVLSRRYIEKIASSLARESVTTAIDTMNYLIKINSAWKTSKPSVIKTTGGD
                     DKIKEEEISDEELESILALLEAKKD"
     gene            13547..14512
                     /gene="pfkA"
                     /locus_tag="PLH17_02600"
     CDS             13547..14512
                     /gene="pfkA"
                     /locus_tag="PLH17_02600"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOR20701.1"
                     /translation="MVKRVGVLTSGGDAPGMNAAIRAIVRSGLKNGLEMFVIYEGYKG
                     LIEGNIEKVDRNFVSDIINRGGTILKSARSREFKEEAARLIGVEQLKNFGIDTLIVIG
                     GDGTYMGAKKLSELGINCIGLPGTIDNDLSSSDYTIGFDTALNTICECVDKLRDTTAS
                     HQRCTIIEVMGNKCGDLALFSGVAEGVEMIITPEHPYSESEIMDRIKALGTGKKKHSL
                     IIVSEKVFPNVLSFAEKVTQETGFETRAEVLGRLQRGGSPSAYDRVLATRMGVYAIEI
                     IVKGIRNVVVGVVNGDIVHFTFDEAFALPRKTHEDIHRLISMVNR"
     gene            14515..15366
                     /locus_tag="PLH17_02605"
     CDS             14515..15366
                     /locus_tag="PLH17_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOR20702.1"
                     /translation="MSDERLIISADIGGTNSRFALVNEDYEIVRSVSRRTILYKKDEF
                     VASIITAIKDLGGNFENIIGLSLGIPGPIKDGGYVVDLPNIHIQDIPLGDILRHEFGL
                     PVFIRNDAEMACFAEASLGSGKSYRRVFFITISTGLGGALVTDKNFDETPFEIGHTPY
                     TYKGEKKFYEYFASGTGLANLARMYDLEINNSLHFFELVVAKDRKALLVYKEWLAILG
                     DFLAYIKSNYHPDIIAITGGVFNSKDVFWNDLIKLHPDLNLQECYFFQNAGIIGSASY
                     GFSMLNN"
     misc_binding    15393..15587
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15619..17520
                     /gene="thrS"
                     /locus_tag="PLH17_02610"
     CDS             15619..17520
                     /gene="thrS"
                     /locus_tag="PLH17_02610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOR20703.1"
                     /translation="MKLILPSGDELAIESGLSGYDAASRISVSLAKKALAYRLDGKLF
                     DLHSKIPQDGHFEVVTEGDKEAFELLNHSTSHLLAHAIMRLYPDAHIGFGPSIDEGFY
                     YDIDFPVTITDADLIAIENEMHKVAKENHRIIRREVSLEEALKLFAKNPYKVELIREI
                     GANISIYEQGDFFDLCTGPHLPNTSFVRHFKLTSLAGAYWRGNSDNKQLTRIYGTSFF
                     SKEELDKHLAILEERKKRDHRRLGKELGLFMVSEYGPGFPFWLPNGMILRNELENFWY
                     KKHEQEGYVFIKTPTMLSKELWITSGHYENYRENMYFSQIDDREFAIKPMNCPGSLLV
                     YKNELHSYKDFPIRVGELGLVHRHEASGALSGLFRVRAFTQDDAHIYCREDQSVAEVV
                     RMLKLYDEVYSIFGLSYHIELSTRPEKKYIGSIENWNRSEKALADACKTIGRDYIVNE
                     GDGAFYGPKLDFKLRDSMGRIWQCGTIQFDMNLPERFDITYIDSRGEKVRPVMLHRAL
                     FGSLERFIGILIEHYGGAFPTWLAPVQVNLIPVNNSLHSQYAHSVASYLRGLGIRASV
                     DDSEEKVGFRLRNSQVRKIPYTIVIGDNEMRDQTVTYRLYGQEKQVTVSQGDFAGLIS
                     DEITSKRHY"
     gene            17722..18261
                     /gene="infC"
                     /locus_tag="PLH17_02615"
     CDS             17722..18261
                     /gene="infC"
                     /locus_tag="PLH17_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOR20704.1"
                     /translation="MNPGQKRPEANNDLVNERIRFPEVLVIGPDGGSLGKMSSRAAND
                     MASRYELDLLCVAPNANPPVCKILNYGKYRFEAQKRAKESRKNQKIIEIKEVQLTPQI
                     GLHDLMVKVKGAIGFLQDGNKVKVGVRFRGRQLAHPEVGKEVLNKFIELVQEYATVEK
                     EGELEGRWMTAILASKIKK"
     gene            18274..18468
                     /gene="rpmI"
                     /locus_tag="PLH17_02620"
     CDS             18274..18468
                     /gene="rpmI"
                     /locus_tag="PLH17_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOR20705.1"
                     /translation="MPKMKSKRALMKRIKVTGSGKVKRHAAYTSHLAPRKTTKQKRHL
                     RKARLVSHSDYKRIKFLIIK"
     gene            18481..18840
                     /gene="rplT"
                     /locus_tag="PLH17_02625"
     CDS             18481..18840
                     /gene="rplT"
                     /locus_tag="PLH17_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOR20706.1"
                     /translation="MARVKGGTVTRARRKKVLKRAKGYFGSKHLLFKTAKEQVMHSLM
                     YAYIDRRKIKRDYRKLWIKRINAACRMCDISYSRFINGLSLAKVDINRKMLSELAISD
                     FEAFKQLVEVSKKAAVK"
     gene            complement(18907..20085)
                     /gene="thiI"
                     /locus_tag="PLH17_02630"
     CDS             complement(18907..20085)
                     /gene="thiI"
                     /locus_tag="PLH17_02630"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOR20707.1"
                     /translation="MNYDRIMVRYGELSTKGRNKMDFVKLLADNIRKKLADNFADVKL
                     ETRFDHIYVLIGSNDCYAMIKELQEVSGINSLSLVSKIEKDIDQIKRRALDMVLKKEA
                     ATFKVRAKRSDKTFPIISDEINRLVAKEILINTNLKVDVKSPDLTIGIEVRHEGVYLS
                     LETFMGAGGYPLGIAGKVMMMISGGIDSPVAAYLLMKRGVTVECVHFASPPYTNSGVI
                     DKVIDLLKSLARYQEQIRLHIVPFTKLQEAIYDHVDESYAITIMRRMMYRISADLAKI
                     RKCQAIASGESIGQVASQTLKSMRVINEVTNMPVIRPLVTFDKNDIIRISKLIKTYDI
                     SIRPFEDCCTIFRPQNPRTKPNYKSTIWLEQQFDYEALIVEAINGTESRIISANSEDL
                     "
     gene            complement(20082..21212)
                     /locus_tag="PLH17_02635"
     CDS             complement(20082..21212)
                     /locus_tag="PLH17_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOR20708.1"
                     /translation="MIYLDNAATTKPRRELLDIFQKIEGEFFANANSDHQAGREAEKY
                     VAKARSIILDALGVMETHRVVFCSSATEANNLALKGFVFKHKKRGDHLIISNIEHPSV
                     MECACQLRDAFGYDLTILNVNHKGFVRPEDVRQALTKNTILVSVMAVNNEIGSINPIS
                     EIADIVHRHSRAVLHVDASQAVGKISLPYSDIDLLSISGHKINGLKGSGALLIGKNIE
                     LIPLLSGGGQEYGLRSSTISPALAYTLALAVKEAVADEKKHGRFISSLNSLLRSYFLK
                     KENIVVNSPEKASSHILNVSLTDKKASVVVEALSNQGIMISSVSACHAKAASMSYSVR
                     ALGRSEDLSKNTLRISFDYQTTMEDIEVFIQALDDVLERIRS"
     gene            complement(21236..22972)
                     /locus_tag="PLH17_02640"
     CDS             complement(21236..22972)
                     /locus_tag="PLH17_02640"
                     /inference="COORDINATES: protein motif:HMM:NF017926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HOR20709.1"
                     /translation="MLNVPTIILLGTTGIVLIAIGAALILGAVIFLLYKFVIVRHVAR
                     KQVRELERRFEYLHALLIGQDAQYVKRLEIISRTNLLYVELHTQYLKRFKELRDRFDA
                     QAQNTINGLKDLVDEGKYKALKVAYPEGKGIIETYEKHVNTLNSDLISVVKPEEEARQ
                     ASLSLKEELRRIKQDYYSKQGDLQLVISSFDAVYEYIDGLFDEFEQFVESAQYDDANL
                     LLPQISKAIKEVGRVLNKLPNLCALVTTVIPDKIASLMNAYEEMADAKYPLHHLIVKN
                     NIYDMNRELEVLTKKIKSFELNNVNDSLDNIIARIDEYFILFEEERKARVIFDAECED
                     IYVVVNTLEKKFIRLCNTIPEVKKIYVISSGYANRVNEIQSEINKVGATKRSLDTFIH
                     SSTKQPYSILVGKMHELRDESNLALSMMEDFQIYLDSLKATAEGAYELIYDFYHRVKK
                     AEKIVRDISLDKFTEKYGESLDKIYGALNTVNEVLNILPIDIGTVTKNTEWLSDEGDK
                     VINEIESENNMMSLAESAILYANRDRQHLSDIHHLVLQSEAHFYEANFEKAYVDTSNA
                     LRKLRQGGGTSN"
BASE COUNT         6213 a         5556 c         5179 g         6018 t
ORIGIN      
        1 tgttttggtt caatatgcaa ttgaatatag aatgacgtat gattataatt gcaatgatta
       61 ataagaggtc agtcgcatgg taaaaatatt agtagtcaac gccggaagtt caagtcttaa
      121 atataaactc tatgagatgc cggaagaaga agtgatctgc tctggcatcg ccgaacgcat
      181 cggtcacgaa aatgccatct ttcgtcttaa atttaacggc gactccataa agacaatcct
      241 tcccatcaaa gatcacacaa aagccgtcga attgctctta gatgcattaa tcgtccatcg
      301 cgtcatcaat aatttcagcg atattaaggc tgtcggtcac cgcgttgtcc aagggggcaa
      361 atatttctcg catagtgttt tctttgatga tgatacgatt gacaaagtcg aatccttaat
      421 tgaactcgcc ccacttcaca acggaccaca cttaattggc gttcgtgcct ttaaaagcat
      481 tttgcctcat attcctaatg tcgctacctt tgacacggct ttccaccgca gcatggctga
      541 agtcgatgcc atttttccta ttccctacga attgactcag gaattcgata ttcgccgtta
      601 cggagcacat ggaacttccc atcaatatct cgatcaagaa ggtcgcaagt taattcctga
      661 tgtcgaacat ccacgaatca tcacgtgcca catcggctca ggggcctcaa tcgccgccat
      721 caaagatgga aaatgcgtgg ccacatcgat gggtctcacc cctcttggtg gtatcatgat
      781 gggcacccgc actggcgata ttgatccctc ggtcgtctac tttttatgca aaaagctcaa
      841 taagagcatc gatgaggtct acgagattct caataagaaa tccggtcttc tgggcgtctc
      901 gggcgtctcc aatgattccc gcgatgtctt gaaagcgatg gagaatggca acccccgcgc
      961 cgtgctcgcc gatgcgctct ttaatcgccg tatcgccgat tacatcggtc agtactttgt
     1021 ccgcctcggc ggcgccgact taattatttt ctctgccggc atcggcgaaa attccgccta
     1081 cttccgctca agggtcatcg aggagatcgg accggctctc ggcgtgaaaa tcgatgaaac
     1141 aattaatgaa agttcacgcg gtgtctccgt cctcttgtcg acgccggaca gcaaggtcaa
     1201 agtcgccctc atccctacgg atgaagaggt gatgatcgcc cgcgatactt ataatctaat
     1261 ttcaaaatag gtcttcattc ttattacata gtaatactgt tacgataaaa ccgctttcat
     1321 catttccgtc atcttaataa agtaatttat tgttgacatc ttttttgttc gaaactacta
     1381 taatagcaat tttaatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     1501 tttattgttg acatcttttt tgttcgaaac tactataata gcaattttaa tttacaacga
     1561 aaggagaatt gtaatgaaat taaaaaattt actacccgtc acgttgcttg ctgtttttgc
     1621 ccttgcggcc tgcaatccga ccgacacttc tagttcgggc gacacatcaa gcgaaattga
     1681 agaatccgcg ctcaaagaca taattgccgg acttcctctt ggcgaagttg atgaagctgg
     1741 actcacctcg atctatgcta acctcggcga tgtcacactc ggcattcagc agagcgcttt
     1801 cgaaggaatg agcggaattg aaccgcgcat cctaaccgat ggctctcaag gttacatgga
     1861 atacatttcc ggttctggcg ccattgatgg cgatgtcgtc caagttgcag tcgaaactga
     1921 attaagcctt gccgaatccg atggagccgg cggctatgtt ctcggtgaag ctgtccccga
     1981 tattagtgaa gtcacgaccg tttggaaaga tgacaccagt gtctacatgg cgaatgttgt
     2041 caatgaagat cctgccgatg actattcttt cttggccacc gctcccgtcg ctgatgtcga
     2101 tgttgctctc ttcaaagatt tcgtcaatag tggcgattta gcctattatg gcgctcttgc
     2161 cgctgaagct tccaccgctt ttgtcgtacc ggatgatttc gctgaggtca ccttgattca
     2221 aaaagctgac aaattcgtcg attccgaaac tgatgatgtc tatttacacc tcgtaaatta
     2281 cacattaggt tcatggttcg gactttatag cgccgaagcc gttggcaatg ccgccggtcc
     2341 tggcaaatac atcgaccgcg gtgaagtctg gggctttgaa gcctacatta acgctgatgg
     2401 cgttcttgat tcagtttata cctatagtga tcatttcatt aactggactt atcaagattt
     2461 gaactgggaa gagggcgatc ccgctcctag cgatccgctt acaccagccg aaatcgaagc
     2521 tcttgattta gagctcctcg gcgaatcttg ggccttcgtc tatggcgtta tgtatcacga
     2581 attctcatcc gaggctccgg atgcagtcgt cgtcccagcc gttgatggcc taagcgccgc
     2641 tgatggtagc gatgccgctt acctcttcga cccagctgat tacggcttta ctctataatt
     2701 gtttttgaac tagaaaacaa aacaacaaaa gttgaaggcg attcgcaaga gtcgcctttt
     2761 tcttgcgctt ttcttttaat ggcgatactt atcatttaat atatagaagt gctaataagg
     2821 agaaattata tgccatcaaa aatagatgcg attattctag aagaagccaa agcttacaaa
     2881 agagagcata accgcatcat caaaaacatt atcaaatacg accgcatcgc catcttccgc
     2941 cacatcatgc cggattttga tgcgatgggt acccaaatgg ggctctacac ttggatcaaa
     3001 gacaattttc caaataagga ggtccatttt ttaggtgata accacgtcac tttcacgccg
     3061 cggctctttc cagaaaccga gcgtttaaat gacgagtggt tcaaagaacc gtttttagcg
     3121 attatcatcg ataccgccaa cacttcacgc atcgccgatc cccgctggaa aaaggcgaag
     3181 tacaaaatca aaatcgacca tcaccccgat gtcgaagatt tcggtaagac gagcatcgtc
     3241 aacacctcga tggcggccgc cagcgaagtc gtcgttaaca tgctgttgtc agtcaagggc
     3301 gactacgtta tgagtaaaca agccgcttct tatttctata ttgcattagc tggtgattcg
     3361 ggacgtttcc aatataattc tacgacccct catactttcg ccatcgccaa ggaactagtc
     3421 aaaaccggct ttgtcttaag cgacatctat aaaaagatgt attttaaaaa gatcgaagac
     3481 ctccgcgtca ccgcttacgt cctcaataac tttcatgtca gcgagcacgg cgtcgcctac
     3541 tatgtgctga gctccgaagt tcaaaaagag ctgaatatca cgaccgaaag aggtaaggaa
     3601 aacgtcaatc tcttctccaa tatcgatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnaa tatcacgacc gaaagaggta aggaaaacgt caatctcttc tccaatatcg
     3781 atggtatcaa tgtctggtgt tcaatcactg aagacgctaa agacaagtgc taccgcgtct
     3841 cgattcgcag caaagagaag acaattaacc aagtggccgc caagtatgaa ggcggaggcc
     3901 acgcccaggc ttccggcgcc aaactcaaga gcctcgatgt cctgcctcag tttatacaag
     3961 atctcgacaa tttatttgtc gaataggtag attatagatt taaaaaggtg tcggttatcg
     4021 ataccttttt atttcaaata accccttctt tttgctaaga tataatatat gagttttatt
     4081 cccttacacg tctacacggg ctattcgttt ttgcaaagcg gaatcaccga agagaattat
     4141 cttaaagcgc ttaagaagcg cggtttcaag ggctcgggca tcaccgactt taattcgctc
     4201 tccggcgttc cgcattttta taaagcttta aaacaagaga agttaaacct cctcgccgga
     4261 gtcgactttc tcatcgatga caatttactt tccttcttcg ccttaaatga agaaggttat
     4321 cgaaatctcc tcaagcttct ttttataaaa agaaccagaa atctgaccta tgaagaagtc
     4381 gggcgccata aagcgggatt ggcggtcatt ctcgccatcg ctcattcaaa gctctcttcg
     4441 ctatttgaaa ccgaacgcga ccagattccg cactttctcc tgcctatttc caatctcggc
     4501 gaacattttt acttgggcat tgaattcggt gtcgaagaag acgaattatt tgtcgcttat
     4561 atgcgcgatt ttgcccgcac tcacggctat cagaccatcg cctttccgtt tatcagatat
     4621 atcgacaaaa aagacgcgat tgtccttgaa attgctaaag cgattcgcga tgatagcgag
     4681 ttggcgattc aagagctcga aggaagcgaa tttcttctaa caaaagaaga aatagcccgt
     4741 ttctttaccg agcaggaaat cgaaaatacc aaccgattgc tcaagctcag ctcctttcgc
     4801 tttgaagaga agcgcggcca tcttctccgc taccagaaaa gcgacgatat aacaagtgac
     4861 gcttatttaa aaaataaagc atcagaaggt ttgcaaaata gaattcccgt tatcggcgaa
     4921 gaatacctat cccgcctcaa ttacgaactt gatgtcatcg ccaagatggg ttacagcgac
     4981 tattttctca tcgttagcga ctatgtcgcc ttcgcccgcg aacacggcat ctcagtcggc
     5041 cccggacgtg gcagcgccgc cggttcatta gtgagttatg ccctcggcat cgtctcgtgc
     5101 gatccgataa aacacaactt gctgttcgag cgtttcttaa atcccgagcg gcaatcgatg
     5161 cccgatatcg acatcgactt ttccgatgtc cgccgcagtg aagtcatcga ctatctgcga
     5221 agtaaatacg gaggagataa ggtcgccaat atcatgacca ttcagacgat cggcgccaag
     5281 caagccctcc gcgatatcgg ccgcgtctac aaaatcgaaa cccgcgaaat cgatatgatt
     5341 tcgaaattga ttatcgatgc caattcttcg cttcgtgaaa actaccgcaa gaacccggct
     5401 tttaagaagc tggtcgacag cgacaagtat tatcttaata ttgtgagtct cgccgcctta
     5461 atcgaaggtc tcccccgcca gtcaggactg cacgccgccg gcattattct taacgattca
     5521 ccgcttgaag aagtcctgcc cgtcaatgtg gattttaatg gcgattacat cgccgaatac
     5581 gagatgaact acctcgaaga acaaggcttc ttaaagatgg atatcctcgg cctccgcaat
     5641 ctttccatca tcgatgagtg cttatcttta attcgccaaa atcgcggcat cagcctcaat
     5701 tatcaagaaa ttccttttga ggataaaaaa gcgattcgca tcatcaaaga aggccggacc
     5761 atggggcttt ttcaactcga atccgctggc atgaaaaaag ccatctccac tttagaaccc
     5821 gaagtgtttg aagatatcgt cgcccttttg gcgctctttc gccccgggcc gatggataac
     5881 attccgagct atgcccgccg caaaaagaaa aaggagccga tcgtctatcc ctccaaagct
     5941 ctcgaagaaa tcctcgcctc cacctatggc atcatcgtct accaggagca aatcacccag
     6001 attgtccgca agatggccga cttctcctat ggtcaggctg atgtctttcg tcgcgcgata
     6061 tccaaaaaag acagcgccca gctatcaagc cttgaacaaa actttgtcgc cggcgccatc
     6121 aaaaacaact attcgcatag cgaagccaag tccgtctttg atctcatcta caaattcgcc
     6181 gattacggct ttaaccgcag ccactcgctt tgctatgcgg tcctagcctg ccagatggcc
     6241 tacttaaaaa cctattacac accggaattt tacgccgcca ttttgggtag ctcttcaggt
     6301 agcaacgaca caaaattcaa cgagacgctc aatgaagtcc ggaagatggg catcaagatt
     6361 ctcaatcctg atatcaatcg ctcgggtcag accttcatcg tcaaagacgg cgcgctaatc
     6421 tttcccctgt tcgccgttaa aggcattctc ggcgctctcg tcgacgccat catcaaagag
     6481 cgcaataatg gcggaccctt cactgatttc tttgattttg tcgcccggat gatcgcctac
     6541 aaaatcaacc agaatcagat tggcaaacta atcgacgccg gcgctttcga caatttcgcc
     6601 atctcgcgct cgtccttgcg cgccacgatt aacagcgccc tccgctatgg ctcgatgctc
     6661 tattctaagg atggccagat gatcatcgat ttaccatttt ccagcaaacc tcgttacacg
     6721 gaagcatttg atgatcctct tgataacctc gaccgcgaat atgaggctct cggcttgatg
     6781 atatccggtt caccgctcac ctataaacaa gataccatca aaaaatatcc ggttatcgca
     6841 ataagcgatg cccgcgagtc gcaaagcccg acgatgcaga ttgtcggcgt gattcgcacc
     6901 gttaaagtca tcaatacgcg aagtggcacg ccgatggctt ttcttaacat ctatgatgac
     6961 agcggcgaca tcgacatcac catctttgcc aaaacctatg ccttgagcta cgagtttctg
     7021 cagaagaaaa aaatcgtccg cgtcatcggc tacatggacc gcaaacgcga aaatgtcttt
     7081 atcgccaatg aaatcatcgc cctggaggaa taagcatggc taaagtttat gtaatcgacg
     7141 gaaattcctt gctctttcgg gcctattacg ccaccgctta tggtcccaac gccaaagtca
     7201 tgcgcactaa ggagggcgtg ccgacgaatg ccctttttgc ctttggcaac atgatcaata
     7261 aaatcatctc ttcattcaag ggcggagagc atatcatcgt cggtttcgac gtcggcaaaa
     7321 aaacctttcg caacggcgag tttgaagcgt ataaggccaa ccgcaagccg actgacgccg
     7381 acctcgtcgt ccagatgcca ctggcccgcg aactcctgac ttcactcgga atcttcgtct
     7441 acgagcagga aggctatgaa gccgatgacg tgtgcgggac gatcgccaag gtcgccagca
     7501 aagccggtca tcaggtcatc gtctacacct cggaccgcga ttttctgcag ctcatcgacc
     7561 acaatatcgc cattcacatc ctcaagaccg gaatgtcgaa catcatggtg atggacgagg
     7621 tgacgatgcc ccaggaattc ggcatttcgc cagcgcagat tcccgactat aaaggccttc
     7681 gcggcgacgc cagcgacaat ctgcctggca ttcccggcat cggcgaaaaa acggccgtca
     7741 aactcctcca gcaatacggc acgtttgaaa aaattctcga agaagcccaa aacatcgacg
     7801 gcaagatcgg acaaaacctc gtcgagcacg ctaaaatcgg tcagttatcc aagcgtttgg
     7861 cgacgataaa aaccgatatc gagttgcctt ttactctcgc tgatactatg tatcaaggat
     7921 atgtctttga cacgattaac caatttggcc agcgctacga actcaagcag ctcctgaatc
     7981 ggctgccgag caaatacaag attgaatcgg aacgcgatgt gaagattgaa taccagaaaa
     8041 tatcttcgtt taagaatgtt gaattaggta gtaaaatcgc ccttttcatc gacatgaagg
     8101 aagacaatta ttttaaggcg ccgattctcg gactcgccct cagcaacggc gcaaaccact
     8161 attacataaa tctcgatgac gctgtccggg atcaaaattt aaaaacgatg ctcgaggatg
     8221 aaaagatcag caaatcatgt tttgatatca aggcggcgac cgttgccctc caccgcctcg
     8281 gcatcgctct tcgcggcgtg gcttttgacc ttttgctggc cgcctatctc ctcgactcct
     8341 cgctaaaaaa caacgtcgat tcaatcctca actatttcgg cgtcgatgcc cacggaaaag
     8401 atgaccgcga actctcgctt tttgacgaat cgaatccgct gaggaccgcc aaggtcgcct
     8461 acttaacttt aaaactgcaa gcccgcgtca tcgaggaact aaaaaagcac gaggctataa
     8521 aactttacga agacatcgaa atgccgctgg ccctcgtcct ggccaagatg gaaatcgaag
     8581 gctttcctct cgacgcgaag acccttgata caatcggcgc tgactaccgc gaaagcttaa
     8641 aagctttgac atcggaaatt tatactttgg ccggtgaaga gtttaatatc aactcgccca
     8701 aacaagttgg agagattctc tttacaaagc tcgctttacc gagtaaccgc aaggactcga
     8761 catcggtcga tgcattaaaa aagctcatcg accgccatcc gatcgtcgct aaaattctcg
     8821 aataccgcaa atatgccaag ttgttatcaa cctatatcga tggcctcgtc gtccacattc
     8881 acgaagacgg caagttacac gccattttca atcaggccct cacgacgacg gggcgtcttt
     8941 cctcgagcga acctaacctg caaaatatct cgattcgcga tgaagaaggg cgactgattc
     9001 gccgggcttt ttactatcct gataataatt atcagattct ctcgcttgat tactcgcaga
     9061 tcgaactgcg catcctcgct tcattgagcc ggtgccaggc ttttatcgat gtctttaatc
     9121 gcgatgatga tgttcacgcc gcgacggcgc gggccatctt cggcagtgcc gacagcaatg
     9181 cccggcgcaa ggccaaagcc gtcaatttcg gcattattta tggcatttcg gattggggtc
     9241 tggccgaaca acttgaaatt ccgcccaagg aagccaaagg gattatcgat gccttctttc
     9301 aaacttatcc cgaagtcagc gattatcttc agaatgtcgt cgaccaggct tttaaggacg
     9361 gctatgtcgc aaccctcttt ggccgccgcc gctacctccg cgaactgtac gacagcaatt
     9421 atcaggtacg cgaattcgcg cgccgggcgg cgatgaatgc gcccattcag ggcacggctg
     9481 ccgacctcat caaactggcg atggtcaaag tccaagcgat gcttgaaaag aagaactatc
     9541 aaacgcgact cgtgctacaa attcacgacg aactgctctt taaggttcac atcgatgaaa
     9601 ttgataaagt ccgcgcggaa ataagcgaaa tcatggaaaa tgtcatcgct cttgatgtta
     9661 aattaaaagt tgacggcggc gtcggcaaga cgtggtatga cgctaaatag aggaaaaaga
     9721 atatgcccga acttcctgaa gtcgaaacga taaaaaatat cctcgccaaa ctggtgatcg
     9781 gcaagaaaat tttaagtgtt gagatattta atcgcaatac cattgaaggc gatccctttg
     9841 actttgcaaa gacactaaaa gaaaagacga taagtaagtt aacccgaatt ggcaaatttc
     9901 ttatttttca tcttgatgaa gaaattattt ttttatcgca cctgcgcatg gagggaaaat
     9961 acttttatca cgagcaaatg ctcgagactt atgataaaca cgcctgcgtc gtcttcactt
    10021 ttagtgatca tagcgccctt gaatataacg acacgcgcaa attcggtttg atgaaacttt
    10081 cgagcgcggc aacttatcaa acttcgcctc cgctttctaa tttaggtccc gagccttttg
    10141 atatcaaaga tgttaagccc cttttatcca aattggcgcg gaaaacgatt ccgattaaag
    10201 ccgtcctcct cgatcagaca tttatggcgg gacttggcaa tatctatgtc gatgaggttt
    10261 tgtatctctc tttaattcat cccgagacgc cggctgatcg cataagcgaa agtcagttaa
    10321 aagggattat ctccaatagt gtcaaagttc tttcgatggc tatcaaaagc gggggctcaa
    10381 cgattcgcag ttatcatccg agccagggca tcgatggcgg ttttcagacg cagcttaacg
    10441 cctatggcaa agagaatctt ccgtgcccgc gctgcaacca cggaatgcgc aagattttcg
    10501 tcggcggacg cggcacgact ttttgtcccc gctgtcagaa aaacccatcg ctgccgtatg
    10561 tcatcggcat caccggtccg attggatcag gtaaatcatc cgtcttatct ttctttggcg
    10621 agcgcggtta tcataccctg agcggtgaca ccattgtcaa aaatctttat catgataaaa
    10681 aagttcaaga attgattatc aaactgttcg gaaataatgt cgtcgatgaa agcggtaata
    10741 tcgacaagcg cttcatcatc gatgttatca tcgccgacga gtataaaaag cgaggccttg
    10801 aaaaaatctt gcatccgcgc gtcgaaaaag ccgttgttga aacgcttaaa aaagcccata
    10861 atgacgataa aatagccatc gaaatgcccc ttctttttga ggctaagctc gatgactact
    10921 gcgatgagac aatctatgta gatatcgaag atagcattca ggccaaacgc ctcgccgagc
    10981 ggaggcttcc tctcgccacg ttaagagccc tgaacaccgg ctttgacgcc cttcgcgata
    11041 aagaaaaggc gacctatgtc gtcgacaact cgtcgtcttt atctaaatta cataagcagt
    11101 taaaacggat tttgcgttaa taggcggaaa cgccgctcag atcaaattct tcttccgcca
    11161 tctcttttaa aaggcgggcg agctgcttgg cgcggatcgc tccagctttg ctggtctcat
    11221 ataattgggc gatttcatta attgtgaagt cgctagtaaa aaaggtcagg cgctgatttt
    11281 tggcgcgttc gattaatatc ggcaaaacga ttgtatcgcg gataaagtcg tttttgtatt
    11341 cgttgccgaa atcatctaag acgaggaggg gaagactcga cagttcaacg atttttttcg
    11401 caaaagcact cttatcgtta aaggacaaat catgaatctc tttgatgcga tttgcgcagt
    11461 tgataaaggc cgccggagct ttggtgatgg cgataaaatc attgactagg gtggccatca
    11521 aaaacgactt gccgacgcga tgtccgccgc ggctgtaaac ccagcgatga gagttacctt
    11581 gcacgatttc gctgaagagg cgaatgatgc gatttctctc agcggtttta tcaagggttt
    11641 taagcgatga ttctttccac tcttcgggaa aatcggcata gatgtatttg ctgtcgattt
    11701 cgatccgttt caaaattttg tcgcacgggg caaaggagcg ctcgatgatg ccgtcatcgt
    11761 tagccaggcg cagctggaga tgaggtgtcg ctttgcgaca attatcgaca cccgggcatt
    11821 tccggcaata gtcgtaatct tctttaaagg cgatgaactt ggaaatattc tcgcggaccg
    11881 ttttcagtgt cggattcatc gtctgcttga taatttcata aacttcgaca tccttgcgca
    11941 gttcatcgat catctcgtcg atataattat cgagttccgt cttttcaaat aacggtactt
    12001 tgatcttatc cattttcatc aatccttttt cgcttcgaga agggccagta tgctctcgag
    12061 ttcctcatcg ctgatttcct cttcttttat tttatcatca ccgccggtgg ttttgataac
    12121 cgatggcttg ctcgttttcc aggcggagtt tatcttaatt agataattca tcgtgtcaat
    12181 cgcggtcgtg acgctttcgc gggcaagtga ggaagcgatt ttttcaatat atcgccgcga
    12241 tagaacattt ttgtttttgg ccaagacata atcgataaga gcgttgatga cgccatgcga
    12301 gaagccaaag ttcttggaaa gatcatcgac gatatctaag tcggagcggc tgggagccgt
    12361 attattttgt ttgatgcgca aatactcacc cggagccatc gcatccatca gtttaatctt
    12421 gctggccaat tcactatcgc tggtgacatg gcttttttcg cccttgctct ttcgatgaaa
    12481 gaaaggaaat ttgacttctt ccttcgcccg gtcggagacg cgctggaaat taatccgttg
    12541 agctttgtta tcgggatcgt aggcgtcgac gacaatctct gccatcgtca gttcgtcaag
    12601 ggagaaaagg gcggccaacc gctcaatctc cttcaggtct tttttagtta agacctcgcg
    12661 ctttatctgc ccgtttttat caagggcgct gaaaaaggca tcaaaatcaa aagaggtgtc
    12721 gatgcggccg gattttcgcc ctttgacttc gacctttaaa tctttgcgaa agaccgggct
    12781 atcgtaatcg ggatgaaatg tctcgacaaa ggaggaggtg atatcatcaa aacccttcgt
    12841 ctgaaaatcg accttatagg agagggcgag gcgcttggct tctttttcgc cgacatattt
    12901 aatgagcagt cccttaaaga gaacgtcatc gaagaattcg cgaggtgatt tgggggcata
    12961 taaaacatag atatagtaac ggattccgcc ctcttcttta aagaaggtgc gcagtaagcc
    13021 gaccgcctcc aaagcgcggc gcgccgtaaa aaactcaccg gctgacatct gcatcttatc
    13081 gaagagatgg gcgtgatcaa acgtatcgat ttcactcgcc ttcatctttt cgtgaaacaa
    13141 agaaagaaaa atgccgacgc ttttatgacc gattaaaggc tgatagagtt cgcttaaaac
    13201 ttcgcggtcg atgtcgctga ttaatgaagc cggcctcgtc tcgtaataat ctttttcgct
    13261 aatgtaatcc atcgttgcta cctaatacgt cacaaattat agcatgggca agcggacaat
    13321 tgaatcaaaa aagccgacgc catcgctact ttttatgata ttgtgaatag ttttccacca
    13381 tcaaaaagcg gaaacttaag cgatgctatc ggcctttgtc gatgctcatt ggccaaaatt
    13441 ctgatttttt ccacaaattt atgtaatcgt tttcaaattg acaaaatcca tactttttaa
    13501 cgattttcat atataatgaa taagaagcaa tagaggtaat tttattatgg taaaacgcgt
    13561 tggcgtctta acttccggcg gcgatgcccc gggtatgaat gccgcgatac gggcaatcgt
    13621 ccgctccggt ttaaaaaatg gtctggagat gttcgtcatc tacgaaggtt ataaaggctt
    13681 gatcgaagga aacatcgaaa aggtggaccg caactttgtc agcgacatca tcaatcgcgg
    13741 cggcacaatt cttaaaagtg ctcgctcccg ggaatttaaa gaagaagccg ctcgcttaat
    13801 cggcgtcgag caactcaaaa atttcggcat cgacaccctg atcgtcatcg gcggcgatgg
    13861 cacatatatg ggggctaaaa aactatcgga actgggcatc aattgcatcg gtttgccggg
    13921 gacgattgat aacgacttat cgtccagcga ttacacgatc ggatttgata ccgctttgaa
    13981 cacgatttgt gagtgcgtcg ataagcttcg cgacacgacc gcctctcatc aaagatgcac
    14041 aatcatcgag gtgatgggca ataaatgcgg cgatcttgct ttgttttcgg gtgtcgccga
    14101 aggcgtggag atgatcatca cgcccgaaca cccatatagc gaaagcgaga ttatggatcg
    14161 catcaaagcg ctcggcaccg gcaagaaaaa gcactcatta atcatcgtca gcgaaaaagt
    14221 atttcccaac gttttatcct tcgcggaaaa agtgacgcag gaaacaggct ttgagacgcg
    14281 ggcggaggtg ctcggacgcc tccaaagggg cggctcccct tccgcttacg atcgcgtcct
    14341 ggcgacgcgg atgggcgtgt atgctattga aatcatcgtc aagggtattc gcaatgtcgt
    14401 cgtcggtgtg gtcaacggcg atatcgtcca cttcactttt gacgaagcct ttgctctccc
    14461 gcgcaagacc cacgaagaca tccaccgcct aatcagcatg gtaaatagat aactatgagt
    14521 gatgagagat tgatcatcag cgccgatatc ggcggcacca attcccgctt tgcgctcgtc
    14581 aatgaagact atgaaatcgt caggagcgtg tcgcggcgaa caatccttta taaaaaagat
    14641 gaatttgttg cctcgatcat caccgccatc aaggatttgg gcggaaattt cgaaaatatt
    14701 atcggacttt cactaggaat tcccggcccg atcaaagacg gcggatatgt cgtcgatctt
    14761 cctaatattc atattcaaga tattcctctt ggcgatattc tccgccacga gtttggcctg
    14821 cctgtcttca ttcgcaacga tgccgagatg gcctgctttg ccgaagcttc gctcggcagc
    14881 ggcaaaagtt atcgccgcgt cttttttatc accatctcaa ccggccttgg cggagctctt
    14941 gtcaccgata aaaatttcga tgagacgcct tttgaaatcg gccacactcc ttacacctat
    15001 aaaggagaaa agaaattcta tgagtatttc gccagcggaa ccggtctcgc taatctggcg
    15061 aggatgtatg atcttgaaat aaataacagc cttcattttt ttgaactagt cgtcgccaaa
    15121 gacaggaaag ctttgcttgt ttataaagag tggctggcga ttctcggcga tttcttagcc
    15181 tacatcaagt ccaattacca tcctgatatc atcgccatca ccggaggcgt cttcaattct
    15241 aaagatgtct tctggaacga cttgattaaa cttcatcccg atctcaatct tcaggagtgt
    15301 tatttctttc aaaatgccgg catcatcggc tcagccagct acggcttttc catgttaaat
    15361 aattgactaa aactaaatta gtcaatatac ttgtaagcgc aaagacgaag acatgtcgca
    15421 tgtcactttc cgagcagaga ggaaaccatc tggtgcgagg tttctggaaa ataatgcgaa
    15481 accacttctg agccttcaat gaaaaattga acgtcactcg cgttaagaga aataagtgca
    15541 tatcaatgat atgaattagg gtggtaccac gtgaaagcgt ccttatgatg ggcgtttttt
    15601 atttaaaagg agcaagttat gaaattaatc cttccaagcg gtgatgaact cgccatcgaa
    15661 tccggactaa gcggttatga cgccgccagt cgcatcagcg tcagcctcgc caaaaaggcg
    15721 ttggcttatc gcctcgacgg gaaacttttt gatctacatt cgaaaattcc ccaagatggt
    15781 cattttgaag ttgtcaccga aggcgataaa gaggcttttg aactattaaa tcattcgact
    15841 tcccatctac tggctcacgc catcatgcgc ctttatcccg atgcccatat cggttttggg
    15901 ccaagtattg atgagggctt ctactacgat atcgatttcc ccgttaccat cacggacgcc
    15961 gacttaatcg ccatcgaaaa cgagatgcac aaggtcgcta aagaaaatca tcgcatcatc
    16021 cgacgcgagg tttctttaga ggaggctctc aaactctttg ccaaaaaccc atataaagtc
    16081 gagctaattc gggaaatcgg cgctaatatt tcgatttacg aacaaggcga cttcttcgat
    16141 ttatgcactg ggccgcatct gcctaacacg agttttgtcc gccactttaa actgaccagc
    16201 ctggccggtg cttattggcg tggtaactcc gacaacaaac agctgaccag aatatatggc
    16261 acttctttct tttctaagga agaattagat aagcacttag cgattcttga agagcgcaaa
    16321 aagcgcgacc accgccgctt aggaaaagaa ttaggtttat tcatggtttc tgaatacgga
    16381 cccggttttc cgttttggct ccccaacggc atgatccttc gcaacgaact tgaaaacttc
    16441 tggtataaaa aacacgagca agagggttac gtctttataa agacgccaac catgctttct
    16501 aaagaattgt ggattacgag cggccactat gaaaactatc gcgagaatat gtattttagt
    16561 cagattgacg accgcgaatt tgccatcaaa ccgatgaact gccccggcag tttattagtc
    16621 tacaaaaatg agcttcattc ctataaggat tttccgattc gcgtcggcga gctcggctta
    16681 gtccatcgcc acgaagccag cggggctctc tccggcctct ttcgcgtccg ggcttttact
    16741 caggatgacg cgcatattta ttgccgtgag gatcaatcgg tcgccgaagt cgtcagaatg
    16801 cttaaactct atgatgaagt ctattctatc ttcgggctca gctatcacat tgaattaagc
    16861 acgcggcctg aaaagaaata catcggctcc atcgaaaact ggaaccgctc cgaaaaagct
    16921 ttggcagacg cctgcaagac catcggccgc gactatatcg tcaacgaagg cgatggcgct
    16981 ttctatgggc ctaaactcga ttttaaactc cgcgattcga tgggtcgcat ctggcagtgc
    17041 gggacgattc agttcgacat gaatttgccg gagcgcttcg atattactta cattgactcg
    17101 cgcggggaaa aagttcgccc cgtcatgctt caccgcgccc tctttggaag ccttgagcgt
    17161 tttatcggca tcctgatcga acactatggc ggcgctttcc cgacgtggct cgcccccgtt
    17221 caggtcaatc tcattccggt caataattca ctgcacagcc aatacgcgca ttctgtcgcg
    17281 agttatctcc gcggtttagg cattcgcgcc agtgttgatg attccgaaga aaaggtcggt
    17341 ttccgactcc gcaattcaca agtcagaaaa ataccttaca cgatcgtcat cggcgataac
    17401 gaaatgcgtg atcagaccgt tacttatcgc ctttacggac aagaaaagca ggtcaccgtt
    17461 tcgcaaggag atttcgccgg actcatcagt gatgaaatca cctctaaacg acattactga
    17521 ttatgcttga cacgcttgcg tagcaatgtt aagattaccg gcgtagatta aacgtagaaa
    17581 gaagatgcgc ctgcttctca ccagattaac aatgttaatt ggtcaatgct acaaaacgaa
    17641 agttttcaaa taacgggcgc ttgctagcgt ccgttttatt tacaagaaaa aaggagttga
    17701 tcgaccatag ctaatttacc catgaaccct ggacaaaaac gtccggaggc aaacaacgac
    17761 ctcgtcaatg aacggattcg ctttcccgaa gttctcgtca tcggacccga tggcggttcg
    17821 cttggcaaga tgagttcacg cgccgccaac gatatggcga gccgctacga actggacttg
    17881 ctctgcgtcg cgcccaatgc caatccgcca gtatgcaaaa tacttaacta cggaaaatat
    17941 cgcttcgaag cgcaaaagcg cgccaaagaa agccgcaaga accaaaagat tatcgaaatc
    18001 aaggaagttc agctgacacc gcagatcgga ctacacgatc taatggtgaa ggtcaaagga
    18061 gctatcggct ttttgcagga tggcaacaaa gtcaaagtcg gcgtgcgctt ccgcggacgt
    18121 caattggccc atcccgaagt cggaaaagaa gttctcaaca aattcattga actcgttcag
    18181 gaatacgcta ccgtcgaaaa agaaggcgaa ctcgaaggaa gatggatgac cgcgattctc
    18241 gcttcaaaaa tcaaaaaata ggagaaaaac aacatgccaa aaatgaaaag caaaagagct
    18301 ttgatgaaac gcatcaaagt tactggcagc ggcaaggtga aacgccacgc agcgtacact
    18361 tcacacttag ccccgcgtaa aaccaccaaa caaaagcgcc atttgagaaa ggcgcgcctc
    18421 gtgtctcaca gcgactacaa acgcattaaa tttctcatca tcaaatagga ggtgccctaa
    18481 atggcaagag taaaaggtgg caccgtcacg cgtgcccgac gcaaaaaagt tttaaaaaga
    18541 gctaaaggat atttcggaag taaacattta cttttcaaaa cggccaaaga acaagtcatg
    18601 cattctttga tgtatgccta catcgaccgc cggaaaatca aaagagatta tcgcaaactc
    18661 tggatcaagc gcatcaacgc cgcttgcaga atgtgcgata tttcctactc ccgcttcatc
    18721 aatggtttgt cgcttgctaa agtcgacatc aaccgcaaga tgctttccga gttggcaatc
    18781 agcgatttcg aagcctttaa acagcttgtt gaagtcagca aaaaagccgc tgttaagtaa
    18841 taagcaaaaa tatgaaaaag gccgcaattt gcgacctttt tttgtgccta acaattcgca
    18901 aaagagctac aaatcttcgc tatttgcaga aataattctc gattcagtgc cgtttattgc
    18961 ttcgacgatt aaagcctcgt aatcaaactg ctgctcgagc cagattgtcg acttgtaatt
    19021 cggcttcgtg cgcggattct gcggacggaa aatcgtgcag caatcttcaa aagggcgaat
    19081 tgaaatatca tatgttttta tcagcttcga gatgcgaatg atatcatttt tgtcaaaggt
    19141 gacaagcgga cgaataaccg gcatgtttgt cacttcgttg atgacgcgca tcgacttgag
    19201 ggtctggctt gcgacttgtc cgattgactc accgctggca atcgcctggc atttgcgaat
    19261 cttcgcgagg tcggcgctga tgcgatacat catgcgccgc ataatagtaa tcgcataact
    19321 ctcgtcgaca tggtcgtaaa ttgcctcttg aagttttgta aaggggacga tgtggaggcg
    19381 aatctgctcc tgatagcggg ctaatgattt gagcaagtca atgaccttgt cgatgacgcc
    19441 gctatttgta tagggaggag aggcgaagtg aacgcattcg acggtgacgc cgcgcttcat
    19501 caaaagatag gcggcaaccg gcgaatcaat tccgcccgag atcatcatca taactttgcc
    19561 ggcgatgccg agcggataac ctccggctcc cataaatgtc tccaaggaaa gatagacgcc
    19621 ttcgtggcgg acttcaattc cgatcgtcaa gtccgggctc ttgacatcga cctttaaatt
    19681 cgtgttgatg agtatttctt tggcgacaag acggttgatt tcatcgctga tgatcggaaa
    19741 agtcttatcg cttcttttcg cccgcacttt aaaagtcgcg gcttcttttt ttaaaaccat
    19801 gtccagagct cgtcttttga tttgatcaat atccttttca attttgctga cgagagataa
    19861 ggagttgatt ccgcttactt cctggagttc tttaatcatg gcgtagcaat cgttgctgcc
    19921 gatgagaaca tagatatgat caaaacgtgt ctcgagttta acatccgcaa aattatcggc
    19981 taacttcttg cggatattgt cggcgagaag tttgacaaaa tccatcttgt tacgaccttt
    20041 ggtcgacagt tcgccgtagc gaaccatgat gcgatcgtaa ttcatgagcg aatgcgctcc
    20101 aatacgtcgt cgagagcctg aataaaaact tcgatatctt ccatggttgt ttgatagtca
    20161 aaactgattc gcaaagtatt tttggacaag tcctcgctgc ggcctagggc gcgcaccgaa
    20221 tacgacattg aggcggcttt ggcgtggcag gcgctgaccg acgaaatcat gattccctga
    20281 ttggagagag cctcgacgac aacgctggct tttttgtcgg taagcgagac gtttaaaata
    20341 tggctcgagg ctttttcggg actgttgact acgatatttt ctttctttaa gaaataactt
    20401 ctgagaagtg aattgagcga ggagatgaac cgtccgtgtt tcttttcatc ggccaccgct
    20461 tctttgacgg ctagtgccag cgtataggcc aaagccgggc tgatcgtcga gcttcgaaga
    20521 ccatactcct gaccgcctcc gctcaaaagg ggaatgagtt cgatgttttt gccgatgagc
    20581 aacgctccac tgcctttaag cccattgatt ttatgcccgg aaatcgagag cagatcaata
    20641 tccgaataag gaagtgaaat tttaccgacc gcttgactcg catcgacgtg caagacggcg
    20701 cgtgaatggc gatggacgat atccgcgatt tcgctgatcg ggttaatgct gccgatctca
    20761 ttattgacag ccataaccga gacgagaatg gtgtttttag ttaaagcctg ccggacatct
    20821 tcggggcgaa caaagccttt gtgattgacg ttgagaatcg tcaggtcata accgaaggca
    20881 tcgcggagtt gacatgcgca ctccataacg gaggggtgct cgatattgct gatgatgaga
    20941 tgatcaccgc gttttttgtg tttaaagaca aagcctttga gcgcgagatt attcgcttcg
    21001 gtcgccgagg aacaaaaaac gacacgatgc gtctccatga cgccgagcgc atcgaggatg
    21061 atgcttcgcg ctttggcgac atatttttcg gcctcgcgac cggcttggtg gtcgctatta
    21121 gcgttggcaa aaaactcgcc ttcaatcttt tgaaaaatat caagtagttc gcgccgcggt
    21181 ttggtcgtgg ccgcgttatc taaataaatc atgatgccct tatgtaagac aaaacctaat
    21241 tgctcgtgcc accgccctgg cgcaacttgc ggagagcatt gctcgtatcg acataggctt
    21301 tttcaaaatt ggcttcgtag aaatgggcct cactttgcaa gacgagatga tggatgtcgc
    21361 tgaggtgctg acggtcgcgg ttagcgtata agatagctga ttcggcaagc gacatcatgt
    21421 tgttttcgct ctcgatttca ttgatgactt tatcgccttc gtcgctgagc cattcagtgt
    21481 ttttggtgac tgttccgata tcaatcggaa gaatattaag aacttcgttg acggtattga
    21541 gcgcaccata gattttgtcg agggattcgc catatttctc agtgaactta tcaagggaga
    21601 tatcgcggac gattttttcg gcttttttga cgcggtgata aaagtcatat atgagctcat
    21661 aggcaccttc ggccgtcgcc tttaaggagt cgaggtaaat ttgaaagtct tccatcatcg
    21721 acaaagcgag atttgattca tcgcggagtt catgcatttt gccgaccaaa atcgaatagg
    21781 gctgcttggt ggacgagtga ataaaagtat cgagcgatcg tttggtggca ccgactttat
    21841 taatttcgct ctgaatctcg ttgacacggt tggcgtaacc cgagctgatg acataaattt
    21901 tcttcacttc cggaatcgtg ttgcaaaggc gaataaattt cttttcgagg gtgttgacga
    21961 cgacatatat atcttcacat tcggcatcaa agatgacgcg ggctttcctc tcttcttcaa
    22021 agaggatgaa atactcatcg atgcgggcga tgatgttgtc gagcgaatcg ttgacgttat
    22081 ttaattcaaa acttttgatt ttcttggtca gaacctctaa ttcgcggttc atatcataga
    22141 tattgttttt aacgatcaga tgatgaaggg gatatttagc gtcagccatc tcttcgtaag
    22201 cattcatcaa agaggcgatt ttatcaggga tgacagtcgt gaccaaggcg cagagattgg
    22261 ggagtttgtt caaaactctg ccaacttcct taatcgcttt agagatttgc ggcaacaaaa
    22321 gattggcgtc gtcatactgg gctgattcga cgaattgttc gaactcgtca aaaaggccgt
    22381 cgatatattc gtaaacagcg tcaaaagagg agatgaccaa ctgtaaatcg ccttgctttg
    22441 aatagtaatc ctgtttaatt ctccgcaatt cctctttcaa agaaagagag gcttggcggg
    22501 cttcctcttc aggcttgacg acggaaatca aatcgctgtt gagagtatta acgtgctttt
    22561 cgtaggtctc aatgatgcct ttgccttcgg gataggcgac tttgagggct ttgtatttcc
    22621 cttcatcgac gagatctttt aagccgttaa tcgtgttttg cgcctgggcg tcaaagcgat
    22681 cccgcaattc tttaaaacgc ttgaggtact gagtgtgcag ttcaacatag aggagatttg
    22741 tccgcgagat gatttccagg cgcttgacgt attgggcgtc ctggccgatc agcaaggcgt
    22801 gaaggtattc aaaacgccgt tccagctcac gcacttgttt gcgggcgacg tggcgaacga
    22861 tgacgaattt gtagagcaaa aagataacgg cacctaaaat cagcgccgct ccgatcgcaa
    22921 tcaacacgat tccagtcgtg cctaataaaa tgattgtcgg gacgttgagc atgttatttt
    22981 accttgtaaa gattatagca tccatccggg gctatttcaa aaatcatttc gtctttttta
    23041 caagatggta ttttttgtcc taacgcctcg atgagcgtta ccaaaaaggc gtaaagaacg
    23101 tctttttaat taaaccccaa ttgaaaaact aaacgcaact ttataatcga aagtcaaaat
    23161 tgtaat
//
LOCUS       DAOSSL010000016        21101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2693_length_21101_cov_14.737052, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000016 DAOSSL010000000
VERSION     DAOSSL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_21101_cov_14.737052"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..2253
                     /gene="pnp"
                     /locus_tag="PLH17_02645"
     CDS             70..2253
                     /gene="pnp"
                     /locus_tag="PLH17_02645"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOR20710.1"
                     /translation="MKRTFELQFEGRKLAVEVGELAKQADGAVLVRYGETVVLSTAVA
                     SKEPKDSDFFPLTIGYEEKQYAVGKIPGSFLRREGRPSEHATLTARLIDRPIRPMFAE
                     GFRNEVQVVNTVLSVDLDNTPEMSAMLGSSLALSISNIPFDGPIAGVQVGRVDGKFII
                     NPNTEESEKSDIHLSVAGTKEAINMVESGAAEVSEEDMLEALMFGHQAIVKLCEFQEK
                     IIAEVGVAKREIVLYHVSEEIEKDIKDRAEKRLVKAISIFDKLERQNAIDDIQKEIIS
                     DYENRKYDSEKVAKTTVSMVKEVLETIVANEVRRLITEDKVRPDGRKVDEIRPLSAQV
                     DLLPRTHGSALFTRGQTQVLSVTTLGSLKDEQIIDNLTAEDTRRFMHHYNFPPYSVGE
                     VGRMGNPGRREIGHGALGERALSYVIPNEEEFPYSIRVVSEVLESNGSSSQASICAAC
                     MSLMAAGVPIKAMVSGIAMGLVTSGPLDGKHPYTILTDIQGLEDHFGDMDFKVAGTAK
                     GITALQMDIKIRGITRDVLKEALAEAHKARAVVREAMEQAITEPRPEVGKYAPKLDKM
                     RIDIDKIRDVIGTGGKVINDIIAKCDDVKIDIDDDGFVVIYHMNRESINKAKQMIEDI
                     VRVAKIGEVFEGTVTRVEDYGAFVNLFGNVDGLCHVSRLGWSYVANASDVVKVGQKLK
                     VVVSEIDDHGKVKVSHREFTEKPENYVERPERPAFNKGPKKPFRR"
     gene            2291..3736
                     /locus_tag="PLH17_02650"
     CDS             2291..3736
                     /locus_tag="PLH17_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR20711.1"
                     /translation="MIDVIIIGAGVVGALTSRELAKYSLKVLVLDKENDVGNETTMAN
                     SAIVHSGYDPLPGTLKARFNVAGNAMFDDLADELDFHFGRVGSLTIALSEEQIPVLMN
                     LRKQARLNGVKVELLTPEEALAMEPHLNPEVKGALFAETCGIVDPFNMTAHAMENAVD
                     NGVELHLNEAVTAIEENAADQTFIVTTSKNSYHCKVVINCAGVYADQMAAMVDQPDFS
                     IRPRKGEYLILDHYTAELVNSTIFPLPSEKGKGVLVSPTTSGNYIIGPSSEFVDEKDD
                     LATDKPTIDKVKKSALDLVPSIPFNETIRAFAGLRASSSRGDFIVEEMKGHEHFINVA
                     GIDSPGLASSPAIAKYVVEELVAKHLPLNKNPNFNPRIKPYINLKALSFEEKNELIRE
                     NPRLGQIICNCEKISLGELDDLMSRSIPPRTIKAVKKRTRAGFGKCQGGFCQPAIVKY
                     LADFYKVDLTDVLYDKGGSNIIRCSTKECKL"
     gene            3736..4989
                     /locus_tag="PLH17_02655"
     CDS             3736..4989
                     /locus_tag="PLH17_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR20712.1"
                     /translation="MARDLVIIGGGSAGMAAAIAAYDEGLTDILIVEKGRQLGGILNQ
                     CIHHGFGIEEFKEQLTGPEFMSRLAVEVKKRNIDVRFQSNVLDLTKSKVVTYSNAAEG
                     IVKVQAKAVIMATGCYERSAGAIQIPGERPSGIITAGTAQLYLNEMGALVGKRVFILG
                     SGDIGLIMARRLTLEGATVLGVSEIMPYSNGLNHNMVQCLHDFDIPLYLSHTVTKTYG
                     KSRLEKIEISQVDEKFNPIPGTEKEFEVDTLLLSIGLIPSNDLLKKLGLPVSKSRGAI
                     VDEHYMTDIPGIFSTGNVLHVHDLADYVAAESRSCGKGAAAYVQGKWNLDSPSFNVIP
                     GKNLLYVVPHRVRLANLDEHLTIKFRVSKPLQNQVLTFTFNGEVIKKIIKPFIIPSEM
                     QKVRLPKELFNRQDGELVISSEDRP"
     gene            4986..5330
                     /locus_tag="PLH17_02660"
     CDS             4986..5330
                     /locus_tag="PLH17_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOR20713.1"
                     /translation="MKELICILCPRGCRLKVDDDLNVTGNFCPRGILYAKTEMTNPTR
                     IITSTVKIKAKNAVRLSVKTAQAIPKGKMFDCMRELDKVMVEAPIRIGDVIVYNICET
                     GVDVVATKDIEE"
     gene            5334..5870
                     /gene="yfcE"
                     /locus_tag="PLH17_02665"
     CDS             5334..5870
                     /gene="yfcE"
                     /locus_tag="PLH17_02665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOR20714.1"
                     /translation="MKVLIASDIHGSMFYAEELKRVIESEAPDIICLLGDFLYSGPRN
                     PVREDYNPKAVVEILNSYADKIIAVRGNCDSDIDAEVLTFPLPRHNEVVINNRRLVLT
                     HGDDMDLAKLNLKKGDILLYGHFHRPQMAFSDGVLALNPGSLTFPKDDYPHTYMVLDE
                     KEVQLRDLSGRTLEVKSI"
     gene            5881..7557
                     /locus_tag="PLH17_02670"
     CDS             5881..7557
                     /locus_tag="PLH17_02670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOR20715.1"
                     /translation="MEDKIRVFALGGLDEDGKNLQVVEINSDIFVIECGIRFPDKTTP
                     GIDYVIPDYDYLKKNKERVRAYILTHGHDDEIGALPYIHKEVPAPIYGSDVTLVMVRS
                     FFEHIRFDASRLDLRVVKPTSDVVIAGRMFHFFHTAHNIAESMGVAIETSLGNVVFTG
                     DYVVENNASENYLHDMNAIARIAEKETVLLMTESSYADRLGYTAPKYKITPLLSSFIE
                     EVKGRLFIALFSRNIYNIDEIINLAVKTRKKIAFYDSETADIFKDMQKVNQLIIPKYN
                     FVSIEDINRVRAGETIVLMCGFGDRLFKKIATLANGLQEDRRIVLSADDTFVVAAPAS
                     PNVEAIATSAIDDLYRSGVKVRALSKKEIVKMHASEEDIKMVVATLKPKYYLPVKGSY
                     RHLLSNAQLVLGMGLRLSHQNVFVLDNGMVLEITRERARILPESVKVGDLLIDGKGIG
                     DVEGGVINERQQLSDDGVVVLGVTISKSRREVVAGPDVQMRGFVFLKDADSILKEVTK
                     IFLTAVTDGLKRPNYRLLDIQNSASEAIIRYIRRANGKNPMVLPLIIEID"
     gene            7638..10304
                     /locus_tag="PLH17_02675"
     CDS             7638..10304
                     /locus_tag="PLH17_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOR20716.1"
                     /translation="MKKSREIQPINKESMLGVIGLMIALIAVIAILSPLPEATRWITI
                     GSAYAFGQIGFWVFYIALFVFGIYLMFKRKLMKIRFDITMIGIIVFLIAFLTFFTIVF
                     VEETLDMKLDTFYAVFQANIQSVILNPGSMPYSVETGGGFLGFLLTALFNYATHIGAI
                     ILSVAFLMTGVGLILNRQVKKLYLKIKEARLKARAKKAYFQSVKNAKPEKFDDIEFKE
                     APKPTPVEPVKEVVSVPVRQERPFYNTPRAIPQPEPIREVKQEAPKFEPRVDSKPAVQ
                     PSAAQALPPYRPGNGDVAFQVKSFSASQGLRKAKFFLDGDAPAHEPTPAYEPAVKTSA
                     VPEVKPVFEPVLEQSANPLKEEINEAKKPESELKTTSMNKPIKEEIGKPEIKIREYTL
                     PSLDLLDNHESEEDLLKNEESTAARTELINTIFEDLNIGAKVVGHTIGPSVTRYDIQM
                     DRNVSVSSMARYINDISIRLGGVATRFEALVIGKATSGLEIPNEYRTNVGLKEALEKM
                     PTGGRFNRDIPFGKNISGELIYANLGDLPHMLVAGSTGSGKSIFVHTLLMSLIMRNRP
                     DELKLLVVDPKRVELSNYADIPHLICPIITDSRKAKVAFNKLVDEMERRYSVFQEARV
                     SKISQYNDSAKSKGLPLMPYIVVVVDEYADLSDTCRDIHEPVVRIAQKARAAGMHLII
                     STQRPTADVVNPRIKTNLPAHVALMMSNFTDSTTIIGESGAEKLLGNGDMLVECAIIS
                     RTSKPRLQGCFVDLVEINRVAEFIKAQWPAEYDPSFLDLEEKVEEFKAPSGEDLLANK
                     ATREMAEEELYAQLVEDIMLREYTSISYITRTYGVGFPKAGRLFARLQRDGIVATEND
                     ASKGCKVLIHDANFNAPSINPGSTELVVEEDK"
     gene            10305..10622
                     /gene="acpS"
                     /locus_tag="PLH17_02680"
     CDS             10305..10622
                     /gene="acpS"
                     /locus_tag="PLH17_02680"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOR20717.1"
                     /translation="MGIGIDIVDMRRIKDAERFAEGILSVRELALMAAKPDKLSFVAG
                     RFAAKEAFMKALGQGLDQIPLKAIEVLYRGPNMAPYVLFNNVEYDVSIAHDGDYAIAT
                     ASI"
     gene            10619..12760
                     /gene="pulA"
                     /locus_tag="PLH17_02685"
     CDS             10619..12760
                     /gene="pulA"
                     /locus_tag="PLH17_02685"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOR20718.1"
                     /translation="MMKRIVKARLITPTQLKVDVFPLSDLEHVVFDLFVNGEKVLTLA
                     NKQHIKNIHEHYFIIDLHYEVVLGNDYKLYCHELGFLNIDISFLAQSDDFDDKYAYEG
                     DDLGASYSPSATSFAVWAPLASKVRLKYSHNRKWCYVDMERTERGVYRAAVKGNLDGT
                     PYLYEITNGGEAVEVSDPYAKLSLQNNRASVVNNPKKYQMAMNEEYLTPFNKYTEAIV
                     YEAHVRDMTIDENSDIVHKGTFKGLAEKNRTTSGGHPAGFDYIKMLGITHLQLLPIFD
                     YATVDEENPDTTYNWGYDPAQYFVPEGSYASKPSDPYSRIIDLKKMVAAYHKEGIRIV
                     MDTVFNHVYHHQTSVFERVVPNYFFRREKNGEIAQRSFCGNDVASERAMARKLIVDAC
                     VYYVKEFGVDGFRFDIMGLIDLETMQIVRDRVKAIKPDFIIYGEGWQMGDESTQDFLM
                     ASFTNAMDMKDIGFFNDWYRDIARGKTSDHDQTPRGYLLNSDGHRDGFKYCYLGSSFD
                     KYISRRFIDPQQSINYVECHDNGTIFDKLEVAMPEEGIEARLDRLKMINAAVVASFGI
                     PFIHMGQEIGLSKKNWQNTYKAGDDLNKMNYAVLDERFHLALHLRDAIKLRRKLSFLQ
                     ESDLAKIEQTVTFEDLDHNALKVSYDLGAYESEFKDFLVFINPSAETIYYDLDEYYQI
                     LFASGGNVLASNTFVKHVMLRGRSFLILILK"
     gene            12820..13107
                     /locus_tag="PLH17_02690"
     CDS             12820..13107
                     /locus_tag="PLH17_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20719.1"
                     /translation="MISDVFLTGRLSEPVSATVRLVEIERMIPEQGKFVIDKIPVLYW
                     SKEPRSRFMKLTTGSLVVIRGRIQIEGAIGLVIVAEQLAILGQNRLNELRG"
     gene            13112..13882
                     /locus_tag="PLH17_02695"
     CDS             13112..13882
                     /locus_tag="PLH17_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOR20720.1"
                     /translation="MLKYVRLILVCGPRIVWSYFAWMIKYARHPERYPLEKRYERIRH
                     LVLFVFKHFHLDVHIEGEENLINLQEPFVVYANHYSYFDPLLLIAISKRPLTFVSKKE
                     ASKYPFIGKVVKALEGISIDRGDLRSQLMMIDHAVKMIESGRNMAIFPEGTRNRNPEM
                     VLGEFKPGAFKLSYRTGAAILSCAIYGTSRPLSSKSKLKVYPIWIRFFKPLEKDEFLQ
                     YSTTYLAPRIQNEIQTEIFDSMMKQDIKYLEDKKSTAK"
     gene            complement(13891..15174)
                     /locus_tag="PLH17_02700"
     CDS             complement(13891..15174)
                     /locus_tag="PLH17_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20721.1"
                     /translation="MDFWTHLATNFPPETAAQIRRSLSDERTRGLLLNTNKIDIREFY
                     SRFPLLSHHPLVSNGFLYDQNTYDFGKLFWHDLGAYYLQEPSAMSVVSFLDIKAGDSL
                     LDLCAAPGGKTMQASLLLGEGGIILANDISHSRALILSSNVERLGLGNVVVTNNDFQT
                     IYQSYLATFDKIILDAPCSGSGMFRKDEKMLKDWSYNKVMACQKKQIELLHIAYLMLK
                     PGGTIAYSTCSFSKEENEDVITCLLSNSDAYLASITGKQGLYSSRHLDGPVYFLPSLF
                     TGEGHFLALIKKPGQLRASQQRNNPYRGKYLALVRQYGLEKRHNLEIDDSLYSLSLPF
                     DVKGLNVLRYGVRCFDEISKHPQPNHHLARFLGATQSLVLDDVSFREYLAGEMLNIEK
                     TPGYHLVSYQGLNAGFVRAVGGKLKNLYPKGLRRR"
     gene            15258..16559
                     /locus_tag="PLH17_02705"
     CDS             15258..16559
                     /locus_tag="PLH17_02705"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOR20722.1"
                     /translation="MRMVDIIVKKRDGKILTKEEISFFVKGYVNGSIPDYQVSALAMA
                     ILFRGMTKEEITVLTDLMMHSGDIIDLSSIKGVKADKHSTGGVGDKTSLVLGPMVAAC
                     GVKVAKMSGRGLGHTGGTLDKMEAIPGMKIAIPEEQFMRQVNEVGLAIVGQTGHLVPA
                     DKKLYSLRDVTGTVESIPLIASSVMSKKLASGSDTILLDVKFGSGAFMKDLQSARELA
                     RTMVDIGDGLNRDTRAILTDMEQPLGLAVGNALEVKEAIATLHGKGPHDLVELCLEAG
                     AIMLEQAKVVKDLKMGRQMLEKAIADGSAFNKLKEMVRAQGGDVSFIENPEKFPLARH
                     IVEIRAEEEGYVRRIEALEIGESAMRLGAGRETYDDVIDMSSGIVLAKKVGDFVKAGD
                     VLCVVHTNKNDFAAILEDIRQAFKLSKEKLAPLPIVHDYIR"
     gene            16565..17020
                     /gene="dtd"
                     /locus_tag="PLH17_02710"
     CDS             16565..17020
                     /gene="dtd"
                     /locus_tag="PLH17_02710"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOR20723.1"
                     /translation="MRIVLQNVKESHLSIANRLFCSIGPGYVLLVGFCEGDDEHLAIK
                     MAERILKMRVFQDEKGKTNLSIIDIEGSILAVPQFTLYADLSGGRRPSFTKALEPRAA
                     KGLFDTFVKALKDSYPDVGQGVFGADMQVNLTNDGPFTLILDSRDLFPL"
     gene            17017..18153
                     /gene="mnmA"
                     /locus_tag="PLH17_02715"
     CDS             17017..18153
                     /gene="mnmA"
                     /locus_tag="PLH17_02715"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOR20724.1"
                     /translation="MKTRVMLGLSGGVDSAVAAYLLKEQGYEVVACFMRNWDALANND
                     YLGNPTVNDQQCPQEKDYDDAASVAKALDIPLLRIDFVKEYWNDVFTYFLEEYRAGRT
                     PNPDVFCNKFIKFDAFYKFALENDCSSIAMGHYARRVEKDGHYFLSKAKDLNKDQTYF
                     LSQLSEAQIKACLFPLGELSKEEVRRIAAKLGLSVAFKKDSTGICFIGERHFKEFLHN
                     YLPAQKGDIVDISTRQIIGSHSGAMYYTIGQHRGLGIGGISNQESEGWFVVSKDVEKN
                     IVYVASGSQSQYLYSNYCLVTKLNLFPHYDIDGKKLKAKFRYRSQDADVAIQRIDDDT
                     LKVVFASKQKAIATGQAAVFYDGSLMVGGGLISEVYAGDIRIDK"
     gene            complement(18156..18956)
                     /locus_tag="PLH17_02720"
     CDS             complement(18156..18956)
                     /locus_tag="PLH17_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012938974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20725.1"
                     /translation="MLDIINLDKTFVLDGQETNKKHAIDHLSLRIEEGDFITIIGSNG
                     SGKSTLLNLIAGSIEPDEGQILLDNENIVTLKNHRRSRFIGRVFQDPMLGTIGDMMVE
                     ENMFLANHRGKNTGLAWGLKKKQREAFRNMLEPLGLGLETRLGERMKNLSGGQRQSIT
                     LTMATMNSPRLLLLDEHTAALDPETAKIVMQITDDIVKKAHLTTIMVTHNMRDAIRYG
                     NRLIMMADGKIIFDARDEEKAQLTVEELIKKFSEKTGDVLPDTVILNK"
     gene            complement(18949..19905)
                     /locus_tag="PLH17_02725"
     CDS             complement(18949..19905)
                     /locus_tag="PLH17_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20726.1"
                     /translation="MNIFYNIFTNSLNLGLIYALLGIGVYIAYRVLDIADLGAEGVFP
                     LGGAITVLMIVLGVPPVVAVLIAFIAGGIMGLLTGVLHTKLKIPSLLSGIITMVMCIP
                     LAQLINGITNSFESYSRPGDFVGSVTVIRMNTIFTFFRGFLGEWGYILASVLVITLII
                     GLLYWFFGTELGITVRATGKNKVMSRAQGINTDSRVILGLVISNAIVAMSGSLFSQFM
                     LTSNSTFGQGTIVIALASILLGEAIMLFKKTNFAVSLLGVFVGSFAYQLVINLAMRVL
                     NDNGYLKLVQAILFILILCIPLFKKNYANLKKKMKARRNANA"
     gene            complement(19913..20881)
                     /locus_tag="PLH17_02730"
     CDS             complement(19913..20881)
                     /locus_tag="PLH17_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOR20727.1"
                     /translation="MKVKPLLLAALAATSMVAVTACDGDVVQIGILKFINIAPLNSAE
                     EGFIKALDDAGINYKLTLKDANADGSLVASSSLTLVGESDLVLGIATPAASGLLSARA
                     IKGNDVPIFFTAVTDPVFSGLMSDDEAPDNNVTGTSDMNPVREQVELFTKLGLGIDKI
                     GMIYCSTETNSVIQKDLAAAAADEFGLDFVFETFTTTDQVAAAMDTLIEAGIDGLYTP
                     TDSTLVSAYSTVKAKADAANLPIIAGEEACLELGAVATLSINYFDLGRITGVMAVEFL
                     SGKSISELPVRRVEDMPLILHVDNAEGIGLTFPAELLAEADEVIDN"
BASE COUNT         5496 a         5342 c         5194 g         5069 t
ORIGIN      
        1 attcatatta ctttctaata tgatgtgtta taattactaa ctgttaaatg aaatgaggaa
       61 atagaattca tgaaaagaac attcgaattg caattcgaag gaagaaaact cgccgtagaa
      121 gtcggcgaac tagccaagca ggctgacggc gccgtgctcg tccgctacgg cgaaacggtc
      181 gtcttatcga cagccgtcgc cagcaaagaa ccaaaagata gcgacttctt cccgctgacg
      241 attggttatg aagaaaaaca gtatgccgtc ggcaagattc ccggctcctt tttaagaaga
      301 gaaggccgcc ccagcgaaca cgccacgttg acggcccgct tgatcgaccg cccgattcgc
      361 ccgatgtttg ccgaaggctt ccgcaatgaa gtccaagtcg tcaacaccgt cttgagcgtc
      421 gatttagaca acactccgga aatgtcggcg atgctcggtt catcgctggc cttatcgatt
      481 agcaacattc cgtttgatgg cccgatcgcc ggcgtccagg tcggacgcgt cgatggtaag
      541 ttcatcatca atccaaatac cgaagagagt gaaaaatccg acatccatct ctccgtcgcc
      601 ggcaccaaag aagccatcaa catggtcgaa tccggcgccg ccgaagtgag cgaagaagat
      661 atgctcgaag ccttgatgtt cggccaccaa gccatcgtca agctgtgcga gttccaagag
      721 aagataatag ctgaagtcgg cgtcgccaag cgcgaaatcg tcctatacca tgtcagcgaa
      781 gaaattgaaa aggatattaa agaccgcgcc gagaagcgcc ttgtcaaagc gatatcgatt
      841 tttgataaac tcgaacggca aaacgccatc gacgatattc aaaaagaaat tatcagcgat
      901 tacgaaaacc gcaagtatga cagtgaaaaa gttgccaaga cgaccgtctc gatggttaaa
      961 gaagtgcttg aaaccatcgt cgccaacgaa gtcaggcgtt tgatcaccga agataaagtc
     1021 cgccccgatg gccgcaaagt cgacgaaatc cgtcccctca gcgctcaggt cgacctcctg
     1081 ccccgcacgc atggttccgc tctatttacc agaggccaaa cccaagttct ctccgtcacg
     1141 acgctcggct cgttaaaaga tgagcagatc atcgataatt tgacggctga agacacgaga
     1201 aggtttatgc accattacaa cttcccgccg tattccgtcg gcgaagtcgg ccgcatgggc
     1261 aatcccggcc gccgcgaaat cggccacggc gccttaggcg aaagagctct ctcctatgtc
     1321 attccgaacg aagaagaatt cccctacagc atccgcgtcg tcagcgaagt cctcgaatcc
     1381 aacggttcct cctcgcaggc cagcatctgt gccgcctgta tgtcgttgat ggccgccggc
     1441 gtcccgatta aggccatggt cagcggcatc gcgatgggtt tagtcacctc gggtccctta
     1501 gacggcaaac acccctatac gattttgacc gatattcaag gtctcgaaga tcacttcggc
     1561 gacatggact ttaaagtggc cggtaccgcc aaaggcatta ccgctttgca aatggatatc
     1621 aaaatccgcg gcatcacccg cgatgtttta aaagaagctt tagccgaagc tcataaagct
     1681 cgcgctgtcg tgcgtgaggc gatggaacaa gccatcaccg aaccgcgtcc ggaagtcggc
     1741 aaatacgccc ccaaactcga caagatgaga atcgacatcg ataagatccg cgatgtcatc
     1801 ggcaccggcg gcaaagtcat caacgatatc atcgcaaaat gcgatgacgt caagattgac
     1861 atcgacgatg acggtttcgt cgtgatttat cacatgaacc gcgaatcgat caataaagcc
     1921 aagcagatga tcgaagatat cgtccgcgtc gccaagatcg gcgaagtgtt tgaaggcacc
     1981 gtcacccgcg ttgaggatta tggcgcattc gtcaaccttt tcggcaatgt cgacggctta
     2041 tgccatgtct cccgcctcgg ctggagctat gtcgccaatg ccagcgatgt cgtcaaggtc
     2101 ggacaaaaac ttaaagtcgt cgtcagcgaa atcgatgatc acggcaaagt taaagtttcg
     2161 catcgcgagt ttaccgaaaa accggaaaac tatgtcgagc ggccggagcg tccagccttt
     2221 aataagggac ccaaaaaacc attccgcaga taacaaggct ctaaagtctt gcctatatag
     2281 aggtcaatag atgatagatg taattataat tggagcaggt gtcgtcggcg ctttaacatc
     2341 ccgcgaactt gccaaatatt cgcttaaagt cctcgtcctt gataaagaaa acgacgtcgg
     2401 caacgaaacg acgatggcca attccgccat cgtccattcc ggctatgatc cgcttcccgg
     2461 aacactcaaa gcccgtttta atgtcgccgg caacgccatg tttgatgatt tagctgacga
     2521 actagacttt cacttcggtc gcgttggaag tctgacaatc gctctaagcg aggagcagat
     2581 accggttctt atgaaccttc gcaaacaggc gcgtcttaac ggtgtcaaag tcgagcttct
     2641 tactcccgag gaagcattag ccatggagcc gcatctcaat ccggaagtca agggcgctct
     2701 ttttgccgag acgtgcggca tcgtcgatcc ttttaacatg accgctcacg ccatggaaaa
     2761 tgccgtcgat aatggcgtcg agctccattt aaacgaagcg gtgacagcca ttgaggaaaa
     2821 cgccgctgat caaaccttta tcgtcacgac aagtaaaaat tcttatcatt gtaaggtcgt
     2881 tatcaactgc gccggcgtct atgctgacca gatggccgcg atggtggacc aacccgattt
     2941 ttccattcgg ccccgtaagg gcgagtactt aattctcgac cactacaccg cggaattggt
     3001 caattcgacg atctttcccc tcccgagtga aaagggcaaa ggcgtcctcg tctcgccgac
     3061 gacaagcggc aactacatca tcggaccgag cagcgagttc gtcgacgaaa aggatgattt
     3121 ggcgactgat aaaccgacga ttgacaaagt caaaaaatca gctttggatc tcgttccttc
     3181 cattcctttt aacgaaacga tccgcgcctt tgccggctta agagcctcat cgagccgcgg
     3241 cgattttatc gtcgaggaga tgaaaggaca tgagcatttc atcaatgtcg ccggcatcga
     3301 ctcgccgggt cttgcctcct cgccggccat cgccaaatat gtcgtcgaag aattagttgc
     3361 caaacacttg ccgttaaaca aaaatccaaa cttcaatccg cgaattaaac catatattaa
     3421 tctcaaggcc ttatcctttg aagagaagaa tgaactgata agagaaaatc cgcgccttgg
     3481 tcaaatcatc tgcaactgcg agaaaatctc gctcggcgaa ctcgatgacc tgatgtcgcg
     3541 ttcgattccg ccgcgcacaa tcaaggccgt caaaaagcgg acgagagcag gttttggcaa
     3601 atgccaaggc ggcttctgcc agccggccat cgtcaaatac ttagctgact tttataaagt
     3661 tgacttaacc gatgttctct atgataaggg cggttcgaat atcatcagat gttcgaccaa
     3721 ggagtgcaag ctatgatcgc gcgcgattta gtaatcatcg gcggcggttc ggccggcatg
     3781 gcggcggcca tcgcggctta cgacgaaggt ttgaccgata tcttaatcgt cgaaaaaggc
     3841 cgtcagctcg gcggtatttt aaatcagtgc attcatcacg gattcggaat cgaggagttc
     3901 aaagaacagc tgaccggccc cgaattcatg agccgcctgg ccgtcgaagt taaaaaaaga
     3961 aatattgatg tgcgctttca atcgaatgtc ttagatctga cgaaaagcaa agtcgtcacc
     4021 tattcaaatg ccgccgaagg aattgttaaa gttcaggcca aagccgtcat catggcgacg
     4081 ggctgctatg agcgcagcgc cggcgcgatt cagattcccg gcgaacgtcc gagcggcatc
     4141 atcacggccg gaaccgccca gctttatcta aacgagatgg gagccctggt cggcaagcgc
     4201 gtcttcatcc tcggcagcgg cgacatcggc cttatcatgg cccgccggct gacccttgaa
     4261 ggagccaccg tcctcggcgt cagcgaaatc atgccttaca gcaacggctt gaaccataac
     4321 atggtccagt gcctgcacga ttttgacatc cccttatatc tttcgcatac cgtcaccaag
     4381 acgtatggca aaagtcgcct tgaaaagatc gaaatttcac aagtcgatga aaaattcaat
     4441 ccgattccag gcaccgaaaa agagtttgaa gtcgatactt tactcctgtc tatcggtctg
     4501 attcctagca acgatctctt aaagaaactc ggcttaccgg tctcaaaatc gcggggcgcc
     4561 atcgtcgatg agcattatat gactgatata cccggtattt ttagtaccgg caacgtcctt
     4621 cacgtccacg atttagccga ctatgtcgcc gccgaatcgc gctcctgcgg aaaaggcgcg
     4681 gccgcctacg ttcaaggtaa atggaatttg gattccccgt cttttaatgt gattccgggt
     4741 aaaaatttat tatatgtcgt tccccatcgg gtgcgtttgg caaacctcga cgagcatctg
     4801 acgattaaat ttcgcgtcag caaaccgctt cagaatcagg ttcttacctt tacctttaac
     4861 ggcgaagtca tcaagaagat catcaagccg tttatcattc ccagcgaaat gcaaaaagtg
     4921 cgcctgccca aggaactttt taaccgtcag gatggcgaac tcgtcatctc ttcggaggat
     4981 cgtccatgaa agaattaatt tgtattctct gcccgcgcgg ttgccgcttg aaagttgatg
     5041 atgacctcaa tgtgaccggt aacttttgtc cgcgcggcat cctgtacgcc aagacggaga
     5101 tgactaatcc gacgcgcatc atcacctcga cggtcaaaat caaagccaag aacgccgtgc
     5161 gcctcagcgt caagactgcg caagccattc ccaaaggcaa aatgtttgat tgtatgcgcg
     5221 aacttgataa agtaatggta gaagcaccaa ttcgtattgg tgatgtcatc gtttataata
     5281 tttgcgaaac cggcgtcgat gttgtcgcca ccaaagatat cgaggagtaa catatgaaag
     5341 ttttaatcgc aagcgacatt cacggctcga tgttttacgc tgaagagctc aagcgggtca
     5401 tcgaaagcga agcacccgac atcatctgtc tcttaggtga ttttctctac tcgggtccgc
     5461 gcaatcccgt ccgcgaggat tataatccca aagcggtggt cgagattctc aatagctacg
     5521 ccgataagat tatcgccgtg cgcggcaact gcgattccga catcgatgcc gaagtcttga
     5581 cgtttccgct tcccaggcac aatgaagtcg tcatcaataa ccgccgcctc gtcttaaccc
     5641 acggcgacga tatggattta gcaaagttaa atcttaagaa gggcgatatc ttgctctacg
     5701 gccattttca ccggccgcag atggcgtttt ccgacggcgt tttagctctc aatccgggtt
     5761 cgttaacctt tcccaaggac gactacccgc acacctacat ggtgctcgat gaaaaagaag
     5821 tgcagctccg cgatttatcg gggcgcacct tagaagttaa aagtatatag gataaaaata
     5881 atggaagata aaatcagagt atttgcgtta ggtggacttg acgaagacgg caaaaatttg
     5941 caagtcgtcg agattaacag cgacattttc gtcatcgaat gcggcattcg ctttcccgac
     6001 aagacgacgc cgggaatcga ctatgtcatc cccgactacg actatctaaa gaagaataaa
     6061 gaacgcgtca gagcctacat tttaacgcac ggccacgatg atgaaatcgg agctttgcct
     6121 tacattcata aagaagtgcc tgctccgatt tacggaagcg atgtgacttt agtcatggtc
     6181 cgctcctttt ttgagcacat ccgctttgat gcctcgcgcc ttgatttaag ggtcgtcaaa
     6241 ccgacgagcg atgtcgtcat cgccggccgc atgtttcact ttttccacac cgcccacaac
     6301 atcgccgaat cgatgggtgt ggcaattgaa acgtccctcg gcaatgtcgt tttcaccggg
     6361 gattacgtcg tggagaataa tgcctcagaa aactatcttc acgacatgaa tgccatcgcc
     6421 cgcatcgccg aaaaagagac ggttctttta atgaccgaat caagctacgc cgaccgcctc
     6481 ggctacacgg ctcccaagta taagatcacc ccgcttttat catcgtttat cgaagaggtc
     6541 aaaggccgcc tgttcatcgc ccttttctcc cgcaacatct ataatataga tgaaataatt
     6601 aatctcgccg tcaaaacgcg caagaaaatc gccttttacg acagcgagac ggccgatatt
     6661 tttaaagaca tgcagaaggt taatcagctg attattccca agtataattt cgtcagcatc
     6721 gaagatatca atcgcgtccg cgccggcgag accatcgtct tgatgtgcgg cttcggcgat
     6781 cgccttttta agaagatcgc aacgctggct aatggccttc aggaagaccg gcgcatcgtc
     6841 ttatcggccg atgacacttt tgtcgtcgcc gccccggcca gccccaacgt cgaagcgatt
     6901 gccacttcgg cgatcgacga cctctatcga agcggcgtta aagtccgcgc tttaagtaag
     6961 aaagagatcg tcaaaatgca cgcctcggaa gaggatatca agatggtcgt cgccaccctt
     7021 aaaccgaaat attacctgcc cgtcaaaggc agttaccgcc acctcctttc caacgctcaa
     7081 ctcgtcctgg gcatgggctt gaggctttcg catcaaaacg tcttcgttct cgataacggc
     7141 atggtcttag aaatcaccag agaacgggcc cgcatcctcc ccgaaagcgt caaagtcggc
     7201 gatttattaa tcgacggcaa gggcatcggc gatgtcgaag gcggcgtcat caacgagcgt
     7261 cagcagttaa gcgatgacgg cgtcgtcgtc ctcggcgtca ccatctccaa gagccggcgc
     7321 gaagtcgtgg ctggccccga tgtgcaaatg cgtggtttcg tctttttaaa agacgccgat
     7381 tcaattctaa aggaagtgac caagattttc ttgacggctg ttaccgacgg gctaaagcgc
     7441 cccaattacc gccttttgga cattcagaac agcgccagcg aggcgattat ccgctacatc
     7501 agaagagcca acggcaaaaa tccgatggtc ctccccttaa tcatcgaaat tgactagttt
     7561 aagcataaat gcttgttttg cccaataaat tctttaattc tttttgacgc gcaagtataa
     7621 tgacttgtat aaaactcatg aaaaaatccc gtgagattca acccatcaat aaagaatcga
     7681 tgctcggcgt catcgggctg atgatcgccc tcatcgccgt catcgctatc ctgtcacccc
     7741 tccccgaggc gacgcgctgg atcacgatcg gttcggccta tgcctttggt cagatcggct
     7801 tctgggtttt ttacatcgcc ttgttcgtct ttggcatcta tctgatgttc aagcgcaagc
     7861 taatgaagat ccgctttgat atcacgatga tcggcatcat cgttttccta atcgcttttt
     7921 taactttttt cacaatcgtt tttgtcgagg agacgctcga catgaaactc gataccttct
     7981 atgccgtttt tcaggcgaat atccagtcag tcatcctcaa tcccggcagc atgccctaca
     8041 gcgtggaaac cggcggtggt tttctcggtt ttcttttaac cgcccttttt aattacgcca
     8101 cccacatcgg cgcgatcatc ctcagcgtcg cctttctaat gaccggcgta ggcttgattt
     8161 taaaccgcca agtcaaaaaa ctttatctta agataaaaga agcgcggtta aaagcccgcg
     8221 ccaaaaaagc ctactttcag agcgtcaaaa acgctaagcc ggaaaaattc gacgacatcg
     8281 agtttaaaga agcgccaaaa cccacacctg tcgaacccgt caaggaagta gtttcggttc
     8341 cggtcagaca agagcgcccc ttttacaata cgccgcgagc gattccgcaa cccgaaccaa
     8401 tcagggaagt taagcaggaa gcgccaaagt ttgaaccgcg cgtcgacagc aagcccgccg
     8461 ttcaaccttc ggccgcccag gccttacctc cttatcgccc cggaaacggc gatgtcgcct
     8521 ttcaagtcaa atccttcagc gccagtcagg gccttcgcaa agcgaagttc ttccttgacg
     8581 gcgacgctcc tgcccacgag ccgactccgg cttacgaacc ggccgtcaaa acatccgccg
     8641 tcccggaagt caaacctgtt ttcgagccgg tcctcgaaca aagcgccaac cctttaaagg
     8701 aagagattaa tgaggctaag aagcctgaat ctgagctaaa aaccacttcg atgaataagc
     8761 cgataaaaga agaaatcggc aagccggaaa tcaagattcg cgaatacact ctgccgtctc
     8821 ttgaccttct cgacaaccat gaatcagaag aagacttgct gaagaatgaa gaatcgactg
     8881 ccgcgcgcac cgaactcatc aacaccatct ttgaagatct caacatcggc gctaaggtcg
     8941 tcggccatac gatcgggccg tccgtcaccc gctacgatat tcagatggac cgcaatgtct
     9001 cggtctcctc gatggcgcga tatattaatg atatatccat tcgtctgggt ggcgtcgcca
     9061 cgcgctttga agccctcgtc atcggcaagg cgacttccgg cctcgaaatt ccgaatgaat
     9121 accgtactaa tgtcggctta aaagaagccc tggagaagat gccgaccggt ggtcgcttta
     9181 accgcgatat cccctttggc aagaatattt ccggcgaact gatctacgca aatctcggcg
     9241 acttgcccca catgctggtg gccggcagca ccggaagcgg caaatcgatt tttgtccata
     9301 ctcttctgat gtccctcatc atgcgcaacc gccccgatga gcttaagttg ctggtcgtcg
     9361 atcccaagcg cgtcgaacta tccaactatg ccgatattcc ccatttaatc tgcccgatca
     9421 tcaccgacag ccgcaaggcc aaagtcgcct tcaataaact cgtggacgag atggaaaggc
     9481 gctatagcgt ttttcaagaa gcccgcgtca gcaagatttc gcaatataat gattccgcca
     9541 aaagcaaagg tttaccctta atgccctata tcgtcgtcgt cgtcgatgaa tacgccgacc
     9601 taagcgatac gtgccgcgac attcatgagc cggtcgtccg catcgctcag aaagcgcgcg
     9661 ccgccgggat gcacctcatc atctcgacgc agcggccgac cgccgatgtc gtcaatcccc
     9721 gcatcaagac gaatcttccg gcccacgtgg cgttaatgat gtcaaacttc accgattcga
     9781 cgacgattat cggcgagtca ggtgccgaaa aattgctcgg taacggcgac atgctcgtcg
     9841 aatgcgccat catctcgcgg acgagcaaac cgcgccttca gggctgtttt gtcgatctgg
     9901 tcgaaattaa ccgcgtcgcc gaattcatta aagctcagtg gccggccgaa tacgatcctt
     9961 ccttcctcga tttagaagag aaggtcgaag aatttaaagc cccgagcggc gaagatctac
    10021 tcgccaacaa agccacccgc gagatggcgg aagaagaact atacgcccag ctcgtggaag
    10081 acatcatgct tcgcgaatac acttcgatta gttatatcac ccgcacttat ggcgtcggtt
    10141 ttcccaaggc cggacggctc tttgcccgct tgcagcgcga tggtatcgtc gcgacggaaa
    10201 atgatgcttc caaaggttgt aaagttttaa ttcatgatgc taatttcaat gccccgagca
    10261 ttaatcccgg ttccacggaa ctggtcgtcg aggaggacaa atagatggga ataggtatcg
    10321 atatcgtcga tatgcggcgc attaaagacg ccgagcgctt tgccgaaggc atcctgagcg
    10381 tcagagagtt ggctttaatg gccgctaagc ccgataagct ctcgtttgtc gccggccgtt
    10441 ttgccgccaa agaggcgttt atgaaggcgt tagggcaagg tctggatcag attcccctaa
    10501 aggccatcga agtcctctat cggggcccta acatggcgcc gtacgtcctt tttaacaatg
    10561 tcgaatatga tgtttccatc gctcacgatg gcgattacgc catcgccacg gcttccatat
    10621 gatgaagcgc atcgtcaaag cccgcctcat aacaccgacc caactcaagg tcgatgtttt
    10681 tccactctcc gatcttgaac acgttgtttt tgatctgttt gtcaacggcg aaaaagtttt
    10741 gactttagct aataagcaac acattaaaaa catccacgag cactacttta ttatcgattt
    10801 acattatgaa gtcgtcctcg gcaatgatta taaactttac tgccacgagc tcggattttt
    10861 aaatatcgat atttcctttc tggcccagtc cgacgatttt gatgacaaat acgcctatga
    10921 gggcgatgat ttaggggcgt cttatagccc gagtgcgacc agctttgccg tatgggcgcc
    10981 cttggcctcc aaggtgcgct taaaatacag ccataaccgc aagtggtgct atgtcgacat
    11041 ggagcggacc gagcgcggcg tctatcgcgc ggcggtcaaa ggaaatctcg acggcacacc
    11101 ctacttatat gaaatcacca atggcggcga agctgtcgag gtgagcgacc cgtatgccaa
    11161 gctttcgctg caaaacaacc gcgcctcggt cgtcaacaat cccaaaaagt atcagatggc
    11221 gatgaatgag gaatatctta caccgtttaa caaatacacc gaagccatcg tctatgaagc
    11281 ccatgtccgc gatatgacga tcgatgaaaa cagcgatatc gtccataagg gaactttcaa
    11341 aggtctcgcg gagaaaaatc gcacgacgag cggtggtcat cccgccggtt ttgattatat
    11401 caaaatgctc ggcatcaccc atttgcagct cttaccgatt tttgattatg cgacggtcga
    11461 cgaagaaaat cccgatacga cttataactg gggttatgac ccagcgcagt atttcgtccc
    11521 ggaaggaagt tacgcttcta aaccgagcga tccctactcg cggattatcg acttaaaaaa
    11581 gatggtcgcc gcttatcata aagaaggcat ccgcatcgtt atggataccg tctttaatca
    11641 cgtctatcat catcagacct cagtctttga acgcgtcgtg cccaattatt ttttccggcg
    11701 cgagaaaaac ggcgaaatcg cccagcgttc cttctgcgga aatgatgtgg ccagcgagcg
    11761 ggccatggct cgcaaattga ttgtcgacgc ctgcgtttac tatgtaaaag aattcggtgt
    11821 cgatggtttc cgctttgaca tcatgggtct gatcgacctc gagacgatgc agattgtccg
    11881 cgaccgcgta aaagccatta aacccgactt catcatctac ggcgaaggct ggcagatggg
    11941 cgacgaaagc acgcaggatt ttttgatggc ctcgtttacg aacgccatgg atatgaaaga
    12001 catcggtttc ttcaatgact ggtatcgcga catcgcccgc ggcaagacga gcgaccacga
    12061 tcagacgccg cgcggctacc tccttaattc ggacggtcat cgcgatggct ttaaatattg
    12121 ttatctcggc tcatcttttg ataaatatat cagccgccgc ttcatcgatc ctcagcagtc
    12181 gatcaactat gtcgaatgcc acgataacgg gacgattttc gacaagctag aagtcgccat
    12241 gcccgaggag ggaattgaag cccgcctcga tcgcctcaag atgattaacg cggccgtcgt
    12301 cgcctctttc ggcatcccct ttattcacat ggggcaggag atcggcctca gcaaaaagaa
    12361 ctggcagaac acctacaagg ccggcgacga tctaaacaag atgaattacg ccgttttgga
    12421 tgagcgtttc cacctcgctt tacaccttcg cgacgccatc aaattgcggc gaaagctttc
    12481 ttttcttcaa gagagcgatt tagccaaaat agaacaaacc gtgacttttg aagacctcga
    12541 ccataacgcc ttgaaggttt cttatgattt aggggcttat gaaagcgaat tcaaggactt
    12601 cctcgtcttt atcaatccga gtgccgaaac gatctattac gacctcgatg agtactacca
    12661 aatcctcttt gccagcggcg gaaatgtcct tgcaagcaac accttcgtca agcatgtgat
    12721 gctgagggga cgatcgtttc ttattttaat attaaaataa atgttgagaa aattatgtgc
    12781 ggaacgccct ttaaaataga ggcggaggta catagttata tgatatcaga tgtctttctc
    12841 accggccgtc tcagcgaacc cgtaagcgcg acggtccgcc tcgtcgaaat cgagcggatg
    12901 attcccgaac aaggcaaatt cgtcatcgac aagattcccg tcctttactg gagcaaagaa
    12961 ccgcgttcac gcttcatgaa gctgacgacc ggaagcctcg tcgtcattcg cggccgcatt
    13021 caaatcgagg gggcaatcgg gcttgtgatc gttgctgaac aacttgcaat tttaggacaa
    13081 aatcgcttaa atgaattaag gggctaaaag aatgcttaaa tacgtgcgtt taatcttagt
    13141 gtgcggaccg cgcatcgtgt ggtcgtattt tgcatggatg atcaagtacg cacggcatcc
    13201 ggagcgctat cccttggaaa agcgctacga gcgcattcgc cacctcgtct tgttcgtctt
    13261 taaacacttt catctcgacg tgcacatcga gggcgaagaa aatcttatta atttgcaaga
    13321 gccttttgtc gtgtacgcga atcactattc ctattttgat cctcttttac tgattgccat
    13381 ctccaagcgg cctttgactt tcgtctccaa aaaggaagcc agcaaatatc ctttcatcgg
    13441 caaagtcgtc aaggctttag aaggtatctc gatcgaccgc ggcgatttgc ggtcgcagct
    13501 catgatgatc gatcacgccg tcaagatgat cgaaagcggc cgcaatatgg ccatctttcc
    13561 cgaagggacg agaaatcgca atcccgagat ggtgctcggc gaatttaaac ctggagcttt
    13621 taaactttcc tatcggaccg gggccgcgat tctttcatgc gcgatttacg gaacttcccg
    13681 gccgctcagt tcaaagagca aattaaaagt atatccgatt tggattcgtt tctttaaacc
    13741 tttggaaaag gatgaattcc tgcagtacag cacaacctat ctagcaccca gaatccaaaa
    13801 tgagattcaa acagaaatct ttgattcgat gatgaaacaa gatattaaat atcttgaaga
    13861 caagaaatcg accgctaaat aataagcctg ctaccttctt cttaaacctt tgggatagag
    13921 atttttaagt ttgccgccga cggcgcggac aaagccggca tttagacctt gataggatac
    13981 gaggtgataa cccggtgtct tttcgatatt tagcatctcg ccggcgaggt attcacgaaa
    14041 tgaaacatca tcgaggacaa ggctttgcgt agcgccaaga aaacgagcca ggtggtggtt
    14101 tggttggggg tgtttgctta tctcgtcaaa gcagcggacg ccatagcgga ggacatttaa
    14161 acctttgacg tcaaagggaa gagaaagaga atatagcgaa tcatcaatct cgaggttgtg
    14221 gcgcttttct aaaccgtatt gacgcactaa ggcgaggtat tttccgcgat aagggttatt
    14281 tctttgctgg ctggccctca gttggcccgg ctttttaata agggccagaa aatgaccttc
    14341 gccggtaaaa agagatggta aaaaatagac ggggccatcg aggtgacggc tgctgtagag
    14401 gccttgtttg cccgtgatcg atgcgagata ggcgtcgcta ttagataata agcaggtgat
    14461 gacatcctcg ttttcttctt tgctaaaaga gcatgtcgaa taggcgattg ttccgcctgg
    14521 tttaagcatc agataggcta tatgaaggag ttctatctgc ttcttctgac aagccataac
    14581 tttgttatac gaccagtctt tgagcatctt ctcgtctttg cgaaacattc ccgacccgct
    14641 gcacggggca tcgagaatga ttttgtcaaa ggtcgcaaga taagactgat agatggtttg
    14701 aaagtcgttg ttggtcacaa ctacattccc gaggccgagg cgttcgacat tgctcgacaa
    14761 gatgagggcg cggctgtgcg agatatcgtt tgccagaatg attccgcctt cgccgagaag
    14821 aaggctcgcc tgcatcgttt tgccgcccgg ggcagcgcac agatcaagga gcgaatctcc
    14881 ggccttgatg tcgagaaagg aaacgacgct catggctgag ggctcctgaa gataataggc
    14941 tcctaaatcg tgccagaaca gcttgccgaa atcatatgtg ttctgatcat ataaaaaacc
    15001 gttgctgacc agaggatgat gggatagaag tggaaagcgt gaataaaatt cacgaatgtc
    15061 tattttgttc gtgttgagaa gcaagccgcg cgttcgctca tcgcttaagg agcgccgaat
    15121 ctgagcggcg gtttccggag ggaaattagt ggccaaatgc gtccaaaaat ccatgttttc
    15181 agtttaacaa gtgcgccggt aaaaagatat taaaacatat ctttgtcatg ttatacttaa
    15241 tagcgaggta aaaacatatg agaatggttg atattatagt gaaaaaaagg gatggcaaaa
    15301 tattgacgaa agaagaaatt tctttttttg ttaaaggcta tgtaaatggt tcgattcccg
    15361 attatcaggt gagcgctctg gcgatggcga ttctctttcg cggcatgacc aaagaagaga
    15421 tcaccgtctt gaccgacttg atgatgcaca gtggcgatat catcgacctc agttcgatca
    15481 aaggcgtcaa agccgataaa cattcgaccg gcggcgtcgg cgataaaacg agtctggtct
    15541 taggaccaat ggttgcggcc tgtggcgtga aagtcgccaa gatgagcggc cgcggcctcg
    15601 gccatacggg tggcaccctc gataagatgg aagccattcc cggcatgaaa atcgccattc
    15661 ctgaagagca atttatgcgc caggtcaacg aagtcggact agccattgtc ggccagaccg
    15721 gacacctcgt cccggccgat aagaaattat attcgctgcg cgatgtcacc ggcacggtcg
    15781 aatcgattcc gttaatcgcc tcctcggtca tgtcgaaaaa actcgccagc ggcagcgaca
    15841 cgattctcct cgatgtcaaa ttcggcagcg gtgctttcat gaaagatttg caaagcgccc
    15901 gcgaattagc ccggacgatg gtcgacatcg gcgacggact caatcgcgat acgcgtgcca
    15961 ttttaacgga tatggagcag cctctcggcc tcgccgtcgg caatgccctc gaagtcaaag
    16021 aggcgattgc gaccttacat ggcaaagggc cgcacgacct agtcgaatta tgcctggaag
    16081 ccggcgccat catgctcgag caagctaagg ttgtcaaaga cttgaagatg ggccgtcaaa
    16141 tgctggaaaa agcgattgcc gacggttcgg cttttaataa attaaaagag atggttagag
    16201 cccagggcgg cgatgtctcg tttatcgaaa accccgaaaa gtttcccctc gcgcgacata
    16261 tcgttgaaat acgcgccgaa gaagaaggtt atgtgcggcg cattgaagct cttgaaatcg
    16321 gcgaatccgc catgcgttta ggcgccgggc gcgaaaccta tgatgatgtc atcgacatgt
    16381 catccggaat cgtcttagct aaaaaagttg gcgatttcgt taaggcaggc gatgttttat
    16441 gcgtcgtcca tacaaacaaa aatgacttcg ctgcgatctt agaagatatc cgccaggctt
    16501 ttaaactatc gaaagaaaaa ttagccccgc tacctatagt tcacgactat attagataaa
    16561 cgctatgcgc atcgtcttac aaaatgtcaa agagagccac ctttcgattg ccaatcgcct
    16621 tttttgctcg attggtccgg gctatgttct gctcgtcggc ttttgcgaag gtgatgatga
    16681 gcacctcgcc attaagatgg ccgaaagaat tttgaagatg cgcgtttttc aagacgaaaa
    16741 aggcaagact aatctatcga ttatcgatat cgaaggttct attttagccg ttcctcaatt
    16801 tactttgtat gccgatttaa gcggcgggcg ccggccttcc tttacaaaag ctctcgagcc
    16861 gagggcggcc aaaggcctct tcgacacttt tgtcaaagcg ctgaaggatt cttatcccga
    16921 tgtcggccag ggcgtttttg gcgctgacat gcaggtcaat ctgacaaatg acggaccgtt
    16981 tactttaatt cttgattccc gggatttgtt tccattatga aaacgcgcgt catgctcggc
    17041 ctttccggcg gcgtcgattc agcggtggcc gcctacttgc tcaaagagca aggatacgaa
    17101 gtcgtcgcct gcttcatgcg caactgggac gcgctagcca ataacgacta cctcggcaat
    17161 ccgacggtta atgatcagca gtgcccgcag gagaaggact atgatgatgc tgcaagcgtc
    17221 gccaaggcgc tcgacattcc tcttttgcgc atcgattttg tcaaagagta ttggaatgac
    17281 gtctttactt actttttaga agaatatcgg gctgggcgca ctcctaatcc tgatgtcttc
    17341 tgcaataaat tcattaagtt tgatgcgttt tataagttcg ctcttgaaaa cgactgctca
    17401 tcgattgcga tgggtcatta cgccaggcgc gtcgaaaagg atggacacta tttcctatct
    17461 aaggccaagg acctcaataa agatcaaacc tattttcttt cgcagctcag cgaagcgcag
    17521 ataaaagcct gtctttttcc cttgggcgag ctgagcaaag aagaagtgcg ccgcatcgcc
    17581 gcaaaactcg gcctttcggt cgctttcaaa aaggattcga ccggtatctg ctttatcggc
    17641 gaacgccatt ttaaggagtt tttacacaat tatcttccgg cccaaaaagg cgatatcgtt
    17701 gatatatcaa cgcgacaaat tatcggatcg cattccgggg ccatgtatta cacgatcggc
    17761 cagcatcgcg gactcggcat cggcggcatt tctaatcagg aatctgaagg gtggttcgtc
    17821 gtcagcaaag atgtcgaaaa gaacatcgtt tatgtcgcta gtggaagtca aagccagtat
    17881 ttatattcta attactgctt agttactaaa ctcaatctct ttccgcatta cgatatcgat
    17941 ggcaagaaac taaaggctaa atttcgctat cgctcgcaag atgctgatgt cgctattcaa
    18001 agaattgatg atgatacttt aaaagtcgtt tttgccagca aacaaaaagc catcgcgaca
    18061 ggacaggcag ctgtctttta cgatggctcg ttaatggtcg gcggcggtct gatcagcgaa
    18121 gtttacgctg gcgatatccg catcgataaa taaaattatt tatttagtat gacggtgtcg
    18181 ggtaaaacat ccccggtttt ctcgctgaat tttttaatta attcctcaac ggtcagctgg
    18241 gctttttctt catcgcgagc atcgaagatg atcttgccat ccgccatcat gatcaagcga
    18301 ttgccgtagc ggatggcatc gcgcatgttg tgggtgacca taatggtcgt caggtgcgct
    18361 tttttgacga tgtcgtcggt gatctgcatg acgattttgg ccgtctcggg atcgagggcg
    18421 gcggtgtgtt cgtcaaggag gaggaggcgc gggctgttca tcgtcgccat cgtcagggta
    18481 atcgactgac gctgcccgcc ggagagattt ttcattcttt caccaaggcg cgtttcaagg
    18541 ccgagaccaa gcggttcaag catatttcga aaagcttcgc gctgcttctt ttttagaccc
    18601 caggccaagc cggtgttctt gccgcggtga ttagccaaaa acatattttc ttcgaccatc
    18661 atgtcgccga tcgtccccag catcggatct tgaaagacgc ggccgatgaa gcgcgagcgg
    18721 cggtgatttt ttaaggtgac gatattttcg ttatcgagga gaatctggcc ttcatctggt
    18781 tctatagaac cagcgataag attgagtaaa gtcgatttgc ccgatccgtt cgagccgatg
    18841 atggtgataa aatcaccttc ttcgatgcgg agacttaagt gatcgatggc gtgtttcttg
    18901 ttggtttctt gcccatcgag gacgaaggtt ttgtcaaggt tgatgatgtc aagcattggc
    18961 attcctcctc gccttcattt tctttttcaa attcgcatag ttcttcttaa aaagcggaat
    19021 gcagagaatg aggatgaaaa ggatggcttg aacgagcttg agatagccgt tgtcgttaag
    19081 aacgcgcatg gccaggttga tgacgagctg ataggcgaac gaaccgacga agacgccgag
    19141 caaagagacg gcgaaattcg tcttcttaaa gagcatgatc gcttcgccga ggaggatgct
    19201 cgctaaagcg atgacgatcg tcccctgacc aaatgtcgag tttgaagtca gcatgaactg
    19261 cgagaagagc gaaccgctca tcgccacgat ggcgttgctg atgacgaggc cgaggatgac
    19321 gcggctgtcg gtgttgatgc cctgagcccg gctcatgact ttgtttttgc cggtggcccg
    19381 gacggtgatg ccgagttcgg tgccaaaaaa ccagtagagc aaaccgatga ttaaggtgat
    19441 gacaagcacg cttgccaaga tgtaacccca ctcgccgaga aagccgcgaa agaaggtgaa
    19501 gatcgtattc atccgaataa ccgtcaccga accgacgaaa tcgccgggtc ggctatagct
    19561 ttcaaaggaa ttggtgatgc cgttaatcag ctgagctaaa ggaatgcaca tgaccatcgt
    19621 gatgataccc gataggaggg aaggaatttt cagtttggtg tgcaggacac cggttaaaag
    19681 ccccatgatt ccgccggcga tgaaagcgat gaggacggcc acgacgggcg gtacgccaag
    19741 aacgatcatg aggacggtga tggctccgcc gaggggaaag acgccttcgg cgcctaaatc
    19801 ggcgatgtct aaaacgcgat aggcaatata gacaccgatg cccaataagg catagatgag
    19861 gcctaagttc agcgagttag tgaaaatgtt gtagaagata ttcatttacc tgttaattat
    19921 cgatgacttc gtcagcttcc gcaagcagtt ctgcgggaaa agttaagccg attccttcgg
    19981 cattatcgac atgaaggata agtggcatgt cttcgacgcg gcgaacggga agttcggaaa
    20041 tgctcttgcc gcttaagaat tcgacagcca tcacgccggt gatgcggccg agatcaaagt
    20101 agttgatgct caaagtggcg acggctccga gttcaaggca ggcttcttcg ccggcgatga
    20161 tcggaagatt agcggcatcg gctttggctt tgacggtcga gtaggccgag acgagagttg
    20221 agtcggtcgg cgtgtataag ccgtcgattc cggcttcgat caaagtatcc atcgcggcgg
    20281 cgacctggtc ggttgtcgtg aaggtttcaa agacaaaatc gagaccgaat tcgtcggcgg
    20341 cagcggcggc aagatctttc tgaatgacgg aattggtttc agttgagcag tagatcatgc
    20401 cgattttgtc gatgccgaga ccgagtttcg taaagagttc aacttgctcg cggaccggat
    20461 tcatgtcact cgtgccggtg acgttgttgt cgggtgcttc atcatcgctc atcaagcccg
    20521 agaagacggg atcggtgacg gcggtgaaaa agatcgggac atcattgcct ttgatggcgc
    20581 gggccgataa gagtccgctg gcggccggcg tggcgatgcc caagacgaga tcgctctcgc
    20641 cgacgagagt gaggctcgaa gaagcgacga ggctgccatc cgcattggcg tccttaagcg
    20701 ttaacttgta gttaattccg gcatcatcaa gcgctttaat aaaaccttct tccgcggaat
    20761 ttagcggggc gatattgata aacttgagga ttccgatttg cacgacatcg ccatcgcagg
    20821 cggtgacggc gaccatcgat gtagccgcga gggcggcgag tagtaggggt ttcactttca
    20881 tttttttctc tcctttatga aataaaactc aggtggcagg cacctgagct tatggtttta
    20941 tactctgtca ggtgtccacg attgctcgtt ttccaccttc tattatcact gataatactt
    21001 aacgggattg gaaaacgatt cctaagtaat aatagtaata ataggaaaaa taggacttct
    21061 ggagcgttaa attgtgattt gcggttttca taaattttgc g
//
LOCUS       DAOSSL010000017        20959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_2716_length_20959_cov_16.668389, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000017 DAOSSL010000000
VERSION     DAOSSL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2716_length_20959_cov_16.668389"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..596
                     /gene="rplK"
                     /locus_tag="PLH17_02735"
     CDS             180..596
                     /gene="rplK"
                     /locus_tag="PLH17_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOR20728.1"
                     /translation="MSKRIAKVVKMELPGGEAKPGPKLASAGVIMPKFCTDFNAKTAD
                     RRGETVPVIITAYEDKSHDFVIKTTPVAELLKKAAGIAKGSANQKTTKVGHISKAKLR
                     EIAEYKMPDLNCNDIEAALKVIEGSAHNMGITIDED"
     gene            640..1323
                     /gene="rplA"
                     /locus_tag="PLH17_02740"
     CDS             640..1323
                     /gene="rplA"
                     /locus_tag="PLH17_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOR20729.1"
                     /translation="MKVGKKYKVVAELVDVNKLYTIEEAAELVKKTSTVKFDATIDAS
                     FRLNVNPKVADQQIRGTINLPHGNGKTKRILAVTNKVEDAKAAGADFAGGKDMLEKIQ
                     KDNWFDFDVIVATPDMMGELGKMGRVLGPKGLMPNPKTGTVAVDIAKAIHEIKRGKVE
                     YRVDKDGNVHVSIARVSFDTTKIAENLQSLCDLIVKVRPSSVKGTYIKNAVIHTTMGP
                     SIKITFAGC"
     gene            1482..1970
                     /gene="rplJ"
                     /locus_tag="PLH17_02745"
     CDS             1482..1970
                     /gene="rplJ"
                     /locus_tag="PLH17_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOR20730.1"
                     /translation="MNNEVLKAKEAVVSELKDKLSNSQTLVVFEYRGMNVQEITALRR
                     ALRAEGTEIGVYKNTLLERAAESLGHKDLLPHLVGPNAFAFSKDVIAGPKVLAKFAKR
                     NEKLVIKGGIVEGKVVSAKQMVALAALPGRDGLISMFLSVLQAPVRQFACAVQGIADK
                     AN"
     gene            1995..2372
                     /gene="rplL"
                     /locus_tag="PLH17_02750"
     CDS             1995..2372
                     /gene="rplL"
                     /locus_tag="PLH17_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOR20731.1"
                     /translation="MAKLTKEEIIASLKEMTVLELNELVKAVEDEFGVTAAAPVAAAA
                     APVEEEEEKKGPTDVNLVLKAVGMSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEH
                     ISPVEAEEHKKALVAAGAEVEVK"
     gene            2677..6240
                     /locus_tag="PLH17_02755"
     CDS             2677..6240
                     /locus_tag="PLH17_02755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOR20732.1"
                     /translation="MSNIDKKHKLLPNDRIDYSKISGTLPLPYLAEIQTESFNWFKEE
                     GLHEVLKEVFPIENYSNDARIRYKECRLEKPDYTYLDCKERDLTYSSALRVKLQLELV
                     KTGEIKEAEVFMGDLPLMTDSGTFVVNGAERVIVSQLVRSPGAYLSKNLEKTGKYTYN
                     ADLIPTRGTWLEFEADQKDLIWVRIDRQRKMSATVLLKALGFEDPKDLVNLFGDRQIL
                     HNTIEKDKDIKTSRDALIEIFRKLKPGEPITNEGVRNFLIQKFFDAKRYDLGRAGRFK
                     YRNKLGIYNRLAGRILAQDLVSHEKEVLFKKDYRLTKEDVDHLRDIEFFEKGAHQKTL
                     KVNKELDSNGIVNVVSVYVNDKKDVKANIIGTDLNQTLSRVTISDIVATFSYFMNVVD
                     GFGDTDDIDHLGNRRIRCVGELIQTQFRIGLTKMAKTVQQKMSISDMESVSVQSLINI
                     RPLTSSIREFFASSQLSQFMDQTNPLAELANKRRLSALGPGGLTRDRASMDVRDVHHT
                     HYGRICPIETPEGQNIGLINNLATYAKINKYGFIETPYRRVDKKAHRVLEESEYLSAD
                     MERTHIIAQANVRLDDEGVILDDFVVARHDGRNIMARKEDVDYIDVSPKQIVSIASAC
                     IPFLENDDTTRALMGANMQRQALPLIKPTAPLVGTGMEHIVARDSGLAVISTGEGVVS
                     YVDSRRIVVKDSKKKTKTYQLQKFARSNQGTCINQIAVVKVGQEVKTGDILADGPAMQ
                     NGDLALGQNVTIAFMTWNGYNYEDAVIMSERLVKDDSYTSIHIEKYECECRDTKLGAE
                     EITRDVPNISEEAKAFLDERGIVVPGAEVKEGDILVGKITPKGVTEPTPEEKLLMAIF
                     SEKTKEGKDTSLRVSHGGAGIVLDVKIFTRKNGDELPPGVNEVIRVYIVQRRKISEGD
                     KMSGRHGNKGVISRILPVEDMPFLPDGTPVDIMLNPLGVPSRMNIGQILEIHLGLACK
                     KLGLKLATPVFDGITNEEIFDLMKKAEIAPDGKTILYDGRTGERFDERISVGVMYMIK
                     LVHMVDDKLHARSTGPCSLVTQQPLGGKAQNGGQRFGEMEVWALEAYGAAHTLQELLT
                     IKSDDRVGRNKTYEAIIKGYPIPRPGIPEAFKVLTKELQALGMDVKLLDNDNNKIDMD
                     ALAKEIQIEERKFNSTIRTLARDGVADTLPTEGDDYSELIN"
     gene            6255..10112
                     /gene="rpoC"
                     /locus_tag="PLH17_02760"
     CDS             6255..10112
                     /gene="rpoC"
                     /locus_tag="PLH17_02760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOR20733.1"
                     /translation="MFDVTKLAALQVGLASPETIRTWSHGEVLKPETINYRSQKPEAQ
                     GLFCEKIFGPSKDFECHCGKYKKKRFQGITCEKCGVEVISKEVRRERMGHIELASPCT
                     HIWYLKGIPSRIGLILDIPPKQLEEINYFVAHVCLDAGTSKVLSYRQFLDERTARQEF
                     VAVIRDFQPTLDPESADYLKSEELIERMQNPTETFDFFTVADFISKYTNAEFGEGAAA
                     IKRLLKEVNLDEEFKIINARLKDCSAQNRIKLSKRLEVIEAFRNSDNRPEWMVLDVLP
                     VIPPDLRPMLQLDGGRFAASDLNDLYRRVITRNTRLKKLIDLNSPYVILMNEKRMLQE
                     AVDALIDNGRRNKPVVGSGNRPLKSLSSSLKGKQGRFRQNLLGKRVDYSGRSVIAVGP
                     DLKMYQCGLPREMAVQLLRPFIASQLMREKHVSSLVQARKYIDRYDPVVYDVLERIIG
                     DHPVLLNRAPTLHRLGIQAFQPKLVDGRAIRLHPLVCTGFNADFDGDQMAVHVPLSKS
                     AQHEALELMLASNNILGPKDGRPIVTPSQDMVLGNYYLTLEETKEQLAARSKRRLDKG
                     DIEEYERFAAYANAEGKVFRNIDEAILAYETHQVHLHNRVAIRASALVKTGFTKEMNE
                     SYLITTVGKIIFNLIFPADFPYLNDASTENLKNDLKKFFVPKGTDISDYIHRQPLVPP
                     FTKKHLGNIINEIFSRYGTSKTSAVLDKMKDIGFKYATIAGVTISISDINVLDNKEKI
                     LGGGEEKVEQINEMFEHGLLTENERHKHVVDVWTDVRDRVQKDLANQFEKDSRNPIFM
                     MMDSGARGNISNFTQLAGMRGLMSNPKGDTIELPIKSCFREGLTVSEFFIATHGARKG
                     GADTALKTADSGYLTRRLVDVSQDVIVREDDCHSDHGHVVSEIIDSSRSTIIVPLYDR
                     LVGRYTVHNVVSPLTGEVIVAENTMIDEESAKRIVDAGIKEVEIRSLFGCQTRDGVCC
                     HCYGRNLATGKEVTIGEAVGIMAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRVQE
                     LVEARVPKGEALISEITGVVNHIEESGGRYTVTIKNELEEKEYTSNYGARLRVKKGDK
                     IRNGGKITEGAISPKKLLEVSDIAAVQRYILKEIQKVYRAQGIGISDKHIEVIIRQML
                     RKVAIIEGGDTNMLPGTLVELDEFTEKNEDALLSGRHPALARPVILGITKASLQTKSF
                     LSAASFQETTRVLTDAAIKAKTDILHGLKENVITGKLIPAGRGLHSDEEEETLVKSFS
                     VQEKMKEVKQQYIEVHDRPDDLD"
     gene            10292..11884
                     /locus_tag="PLH17_02765"
     CDS             10292..11884
                     /locus_tag="PLH17_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20734.1"
                     /translation="MKPNKIPAIAIFSVFILASCGPTVVEDNFYRFNDKEAVATLTQA
                     RKGTYISDMPRSGESRVLVVPVEFSDFPASGMLRGAEGAREDLRKAYFGESEETQWES
                     LSSYYAKSSYGKVNITGQVMPWYRPKNPSKPSQWYSTEEIISSYGSSESTGISRLILG
                     WFYTDYAIDMYQNVLDDEGNPYATAAAFFKDYDGDGDGYIDVIQMVYSAPVNHLSSDL
                     FWAFRSVDSSSAPNKNSPFPCAYVWLGYDFIYENGYYDNLGVYHDWTDAEIIAGTAEI
                     DAHTIIHESGHALGADDYYTYDQKDWGALGGTDMMDYNIGDHNAYTKGIFGWADPIVV
                     TGEATVKVKSFTDTGEAIVLPAYRADGEVSNSLLDNYIMIEYYRPTGLNELDSTYNYT
                     GRYPKMPDVPGIRVYHVDSRLGLFTYGSTSGWQFVRYVSTVTATDSNSYIDIANSNTK
                     SRSADPNNRLIHALEVSGENTLKNTTFNTRYSSSMMWQEGDTFGYDTFVNFTLNNGEK
                     LGYKFEVTDMNDEDVTIKFSLA"
     gene            11990..13447
                     /locus_tag="PLH17_02770"
     CDS             11990..13447
                     /locus_tag="PLH17_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20735.1"
                     /translation="MKNKAFSFFFLITLLTAGCAPTADSSSAPSFSEDPVYDAYYRAE
                     NQELTYYDVSRSGGMDSLPTVGEVNLLVVPVIIEGYEENASNETRSNIQKVMFGQSED
                     TSWESVASFYDKSSYGRLTLTGEVTPWFPSGYSAAEMNAMEARGQDSVSLLLEDAVDW
                     LREEQTTVDLTAYDNDRDGHLDAVWMIYSAPSQISDVFWAFVNWNYRNLNNKNIANPT
                     PFTHAWASFDFMYEGYGTSGIDAHTYIHETGHLLGLDDYYDYDGKTSPMGMIDMMDYN
                     IIDHNGYSKFALGWTNPYIVTGDADINLLPASSSGQSILLTADWNGNPFDEYLLLELY
                     TPDGLNEKDALNSYPANGKRGFTIPGIRMYHADARLFDSETNTYVDDLSASEAYVGAS
                     NSPSWSYLKDYKSKFKLLHVVDAAKNIRYMSSQYARANDDTLFTSDMTFSFEEYKTAF
                     ARGSNLLMNDGTTFPFTIEFSEVSAQGATIKIRVI"
     gene            13489..15144
                     /locus_tag="PLH17_02775"
     CDS             13489..15144
                     /locus_tag="PLH17_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20736.1"
                     /translation="MKRKMLYLAVLSALTLTSCALSFGTTSTDDSSTGSSDTSGDSTS
                     SDTGADTSSGTSSGTSGNTSSDTTSSTGGDTSSSASTATEGWDNYFKPDIVDEYHSYQ
                     DLQKNIYLNSIPSQGEDIDILVVPVEFTDYPFATKTLADLEILFNGTSSQTNYWESVA
                     SFYETSSYGHLDFDFTIAATYDTEKTVSQFVSTYGSFFTTKLVSKVVSDYKTANGNSS
                     TQQFDNDSDGFIDAVWMIYSCPNYSNSDYIANISENFWAYVTWDAQQSYNGSGSVSSP
                     VANVYGWASYDFMYEGGGDTKIDAHTYIHETGHLLGLDDYYNYDEDSDYKPLGAIDMM
                     DYNIIDHNAWSKMALGWLKPYVVTGDAEITINPVESSGDAILIADNWNGTSFDEFILL
                     ELYTPTGLNKLDSRTNYVGRYPRAYTTAGVRLLHIDARLGIFNYSNQFINYGDPGSGP
                     LYNDSTQRYFAMANSNTPSYSADENHRLVHMIQALGTNTFDEGMSGTNSDLFKTSQTF
                     SMSTHGTEFFKNGNKLNNGNALGYAIYFSSVSSESATIRIEKI"
     gene            15158..16663
                     /locus_tag="PLH17_02780"
     CDS             15158..16663
                     /locus_tag="PLH17_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20737.1"
                     /translation="MKPKKMFRSLLIPVFGISAMVLSSCTSVYFAFIPFRDYQVDSDD
                     HETIIEDGYYKPGDYSPYTYLNGDGEEESFANFTDVYRHKDYRRSMRSLGRQKILVIP
                     VDFEDYPSSSLPEGTEGSLEVLRNAFFGINQNNQWRSVAGYYNESSYGKLVLDGKVTD
                     WYRSSKLTADIRNSSATSQIVRDIYNGALAWYQETYGDLASYYVDGDPSKSVPVYLIY
                     THPSETGSGARDKLFWAFTINQKPTLTCWSSYSLTYLSEGRPDTHTYIHEVGHLLGLE
                     DYYNTDSELYGPTGRADMMDYSIGDHTGYSKMLLDWTRPYVVTDTTEITLRPFYNSGD
                     LILIKDNWNGTAMDEYMLLEFYSPNGLNAHDSRLTNSNPQLMQHAGIKVYHVDARLAY
                     YSNDGFRRALGYVEDGVYSQETNRVGIAHSNTQSTTYNNYRLYHLLESGGENTFINGG
                     TATNKTLFKKGDSFGVETFADFTFNGGQELGYTFTISDLANTYAKISITKI"
     gene            16803..17213
                     /gene="rpsL"
                     /locus_tag="PLH17_02785"
     CDS             16803..17213
                     /gene="rpsL"
                     /locus_tag="PLH17_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOR20738.1"
                     /translation="MPTINQLVRQGRVRSTFKSKAPALGKRWNSLQKRQTSQNSAQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNSYEVTAYIGGEGHNLQEHSTVLIRGGRVKD
                     LPGVRYHIIRGTLDTAGIESRRQGRSQYGTKRPK"
     gene            17242..17712
                     /gene="rpsG"
                     /locus_tag="PLH17_02790"
     CDS             17242..17712
                     /gene="rpsG"
                     /locus_tag="PLH17_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOR20739.1"
                     /translation="MPRKGSVSKRDVLPDPIYSSKLVTRLINKIMLDGKRGTAQTILY
                     SAFKKIETKTGKPAMDVFATAINNIMPEVQLKVRRIGAANYQVPTEVTADRKVTLGLR
                     WLVTYARLRNEKTMEDKLANEIIDAANGTGASVKKREDTHKMAEANKAYSHYRW"
     gene            17728..19806
                     /gene="fusA"
                     /locus_tag="PLH17_02795"
     CDS             17728..19806
                     /gene="fusA"
                     /locus_tag="PLH17_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR20740.1"
                     /translation="MAREYDLEHTRNIGIMAHIDAGKTTTTERILYYTGKIHKIGETH
                     EGSATMDWMAQEQERGITITSAATTAFWKGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDGKNGVEPQTETVWRQGTKYNVPRIVFVNKLDAIGADFDMCIETLHTRLAANAE
                     AVQYPIGIESRFNGIIDLINRKAYTYSADKSEGPTEIAIPEEYLEKVEAMRAKLLEAL
                     AAFDDELMLIILEGQEPSIDLIKTTIRKAVLGGEFFPVFCGSAYKNKGVQLLLDGVID
                     YLPSPIDIPHQKGHYSDGREYTCEPDDKQPLAALAFKIATDPFIGRLAYVRIYSGTLK
                     SGSYVINTTKGIKERIGRVVLMHSNHRQEVEELHAGDIGAVVGLKNTTTGDTLCEEKL
                     DVMLEKMEFPEPVIEQAVEPKSKADQEKMGVALQKLSEEDPTFRVHTNSETGQTIIAG
                     VGELHLDIIVDRMKREFGVQVNVGAPQVGYRETIKGSGECEGKYIKQSGGRGQYGHVK
                     IRFEPNPGKGFEFVDNIVGGVVPKEYIRPTNDGLRDSLERGLVAGYPVIDIKATLFDG
                     SYHEVDSSEAAYKIAASMALKEAARTCNPVVLEPIMKVEVTVPEDYYGDVIGDITRRR
                     GQMTGESQRGNAKTVEADVPLSEMFGYVTDLRSFTQGRGNYTMQFSHYGECPRFIQEE
                     IIKKSGMSQR"
     gene            19899..>20959
                     /gene="tuf"
                     /locus_tag="PLH17_02800"
     CDS             19899..>20959
                     /gene="tuf"
                     /locus_tag="PLH17_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOR20741.1"
                     /translation="MAKEKFNRAKTHVNVGTIGHVDHGKTTLTAAITKVLAKSGGAKA
                     TAYAQIDSAPEEKARGITINTSHIEYSTAKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGVMSQTREHILLARQVGVPYIVVFLNKIDLVDDPELVDLVEMDVRDLLSS
                     YGFPGDTLPVIRGSARDALEGDDEACILELMDAVDSYIPDPVRDKDKPFMLPIEDVLT
                     ITGRGTVVTGRVDRGLLKLNDEVEIIGIKPTIKSVVTGIEMFRKLLDFAEPGDNVGVL
                     LRGINRDQVVRGQVLAKPGSVTPHTKFTASTYVLKKEEGGRHTAFLSNYRPQFYFRTT
                     DITGVITLPAGVEMVMPGD"
BASE COUNT         5740 a         5552 c         4846 g         4821 t
ORIGIN      
        1 ctttttattg atattgccaa aattttcttg tatgtgggca tggtatttgt taaaatactt
       61 cagcgtgcgt ttttgtgtgt agacattgtg agcgctcgca agtcttgtgt ggaaggatgc
      121 acgattcatc cctcgaccac aacacggaca acaaattcac tacaaaggag gaatgtcacg
      181 tgagtaaaag aatcgctaaa gtcgtgaaga tggaacttcc gggcggcgaa gccaaacccg
      241 gacccaaact cgcctcagcc ggtgtcatca tgccgaagtt ctgtacagac ttcaatgcga
      301 agaccgctga ccgccgcggt gagaccgttc cggttatcat cacggcctat gaagacaaat
      361 cacacgattt tgtcatcaaa acgacgccag tcgccgaact gttaaaaaag gctgccggca
      421 tcgccaaagg ttccgcaaac cagaaaacta ctaaggtcgg acacattagt aaagccaaat
      481 tgcgggaaat cgccgaatac aaaatgcccg atctcaattg caatgatatc gaagctgctc
      541 tcaaagttat cgagggctca gctcataaca tgggcatcac gatcgacgag gattagtggg
      601 agggtttaga acccgtccgt accacctagg aggaataaaa tgaaagtagg taaaaaatac
      661 aaggtcgtcg cagaacttgt cgatgtcaac aaactctaca ccatcgaaga agctgccgaa
      721 cttgtcaaaa agacttccac cgtgaagttt gacgctacca ttgatgcttc tttccggctt
      781 aacgttaatc ccaaagtcgc ggatcagcaa atcagaggca cgattaatct gcctcacggt
      841 aacggcaaga ccaaaagaat cttggccgtc accaacaaag tcgaagatgc gaaagcagcc
      901 ggcgcggatt tcgccggggg caaagacatg ctcgaaaaaa tccaaaaaga taactggttt
      961 gattttgatg tcatcgtcgc cacaccggat atgatgggcg aacttggcaa gatgggtcgc
     1021 gtcctcggac caaaaggttt gatgccgaac cccaagaccg gcaccgttgc cgtcgacatc
     1081 gccaaagcca ttcacgaaat caaacgcggt aaagtcgaat accgtgtcga taaagacgga
     1141 aatgtccacg tttctatcgc tcgcgtgagt ttcgatacca ccaagatcgc tgagaatctt
     1201 caatcgcttt gcgatttgat tgtcaaggtc cgtccatcaa gcgtgaaggg cacgtacatt
     1261 aagaacgccg tcattcacac gaccatggga ccatcgatta aaattacttt cgctggttgc
     1321 tagtcaaatt ctgaagaagc acaatatacc atagacagca acggccaagg ggcttaatca
     1381 ggttgccgag gtagataaaa atacacccag atacgggttc atcactcata gtatattgga
     1441 gcctcatagt cgaaaaacta acaaaggagg tgaagaacaa gatgaataac gaagtcttaa
     1501 aagccaaaga agcagtcgtc agcgaactaa aagacaaatt gtctaattcg caaacgctcg
     1561 tcgtcttcga ataccgtggc atgaacgtcc aggaaatcac cgctctccgt cgcgctctgc
     1621 gcgctgaagg caccgaaatc ggcgtctata aaaacacgct gctcgaacgg gcggccgaaa
     1681 gccttggtca caaagattta ttgccgcacc tcgtcggtcc caacgcattt gcgttttcca
     1741 aagacgtgat cgccggcccc aaagtcctag ctaaatttgc caagcgcaac gaaaagctgg
     1801 tcatcaaggg tggtatcgtc gaaggcaaag tcgtgagcgc caaacaaatg gtagctcttg
     1861 ccgcattgcc gggtcgcgat ggtttaatct ctatgttctt atcggtgctc caagcaccag
     1921 tccgccaatt tgcgtgtgcc gttcaaggca tcgcggacaa agccaattaa tgccacttta
     1981 ggaggaaaaa tcaaatggct aaattaacca aagaggaaat tattgcctca ttaaaagaaa
     2041 tgaccgttct tgaactcaac gaactcgtca aagctgtcga agatgaattc ggcgtgaccg
     2101 ctgccgctcc tgtcgctgcc gctgccgctc ccgtcgaaga agaagaagag aaaaaaggac
     2161 cgaccgatgt caatctcgtt ctcaaggccg tcggcatgtc caaagtcgcc gtcatcaaag
     2221 ctgtccaggc catcaccggc ttaggactca tggatgccaa gaagttagtc gacggcgctc
     2281 ctgtcaacat caaagaacac atttcaccag tcgaagccga agaacacaag aaagctcttg
     2341 tcgccgccgg cgccgaagtc gaagtcaaat agcacctcga cgcacacatc cgctgacatt
     2401 aggcggattt tcggcttttt agagccttaa aacatcagcg cttaaatagt ggaagcgggg
     2461 gtaaaacctg cccccgcctc taaaggcgca ccctatccga tccgagtcac agccttcgaa
     2521 gtgacataca aacgaaggat taaatgattc aattgtgatt tcccaaacga aagaatggtt
     2581 cggtgaagtc gtcaaattga tggcggtttg cctccgtggg gcaacgaagc gaaagccggt
     2641 tgcaaggcaa acaacaaaag aaaaaggagc cattacatga gtaatattga caaaaagcac
     2701 aaactgcttc cgaatgatcg catcgattat tcaaaaattt cgggaacgct gccgcttccg
     2761 tatctcgctg agattcaaac cgaatcattc aactggttca aggaagaagg tctccacgaa
     2821 gttttaaagg aagtcttccc gattgaaaac tatagcaacg acgcccgcat tcgctacaag
     2881 gaatgccgtc ttgaaaaacc tgattataca tatttagact gcaaagaacg cgatttgact
     2941 tatagttcag cccttcgcgt caaattgcaa cttgaactcg tcaaaaccgg cgaaatcaaa
     3001 gaagctgaag tattcatggg cgatctgccc ctgatgaccg acagcggcac tttcgtcgtc
     3061 aacggcgccg agcgagtcat cgtttcgcaa ctcgtccgtt cgcctggcgc ttacttaagt
     3121 aagaacttgg aaaagacggg taaatataca tataatgctg atttaattcc gacccgtgga
     3181 acgtggctgg aattcgaggc cgaccaaaaa gatctgattt gggtccgcat cgaccgtcag
     3241 cgcaaaatga gtgccaccgt gctgcttaaa gccttaggct ttgaagaccc caaagatcta
     3301 gtaaatctct tcggcgatcg gcaaatctta cacaacacga ttgaaaaaga caaagacatc
     3361 aagacttcac gcgatgctct catcgagatt ttccgcaagc tgaaaccggg tgagccgatc
     3421 accaacgaag gtgttcgcaa cttcctaatt cagaaattct ttgatgccaa gcgctatgac
     3481 ctcggaagag cggggcgctt taaataccgc aataaactcg gcatttacaa ccggttagcc
     3541 ggccgcatcc tcgctcagga tctcgtctct catgagaaag aagtgctctt taaaaaagac
     3601 taccgcctta ccaaagagga cgtcgaccat ctccgcgata tcgaattttt tgaaaagggc
     3661 gcccaccaaa agaccttaaa agttaacaaa gaactcgaca gcaacggcat cgtcaatgtc
     3721 gtcagcgtct atgtcaacga caaaaaagat gtcaaggcca acatcatcgg caccgattta
     3781 aaccaaacgc tttcaagagt gactatttct gacatcgtcg ccaccttctc ctattttatg
     3841 aatgtcgtcg acggttttgg cgacactgac gatatcgacc acctcggcaa tcgccgcatt
     3901 cgctgcgtcg gcgaattgat tcagacccaa ttccgcatcg gcctcaccaa gatggccaag
     3961 accgttcagc agaagatgtc gatttccgat atggaaagcg tcagcgtcca atctttgatt
     4021 aacatccgcc cgctcacttc gagcattcgc gaattctttg cgagttcgca attgtcccag
     4081 ttcatggatc agacgaaccc cctagccgaa ctcgccaaca aacgccgtct ctcggccctc
     4141 ggccccggcg gtctcacccg cgatcgcgcc agcatggatg ttcgcgatgt gcaccacacc
     4201 cattatggtc gcatctgccc gatcgaaaca cctgaaggtc aaaacatcgg tctcatcaac
     4261 aacttggcga cttacgccaa gattaacaaa tacggcttta ttgaaactcc ttatcgccgc
     4321 gtggataaaa aagctcaccg cgtcctcgag gagtccgaat acttgtcggc ggacatggaa
     4381 agaacgcata tcatcgccca ggccaacgtt cgccttgatg acgaaggcgt gattcttgac
     4441 gactttgtcg tcgctcgtca cgatggccgc aacatcatgg cccgcaagga agatgtcgac
     4501 tacatcgacg tcagccccaa acaaatcgtc tccatcgctt cggcgtgcat tcctttcctt
     4561 gaaaacgacg acaccactcg tgctctcatg ggcgcgaaca tgcagcggca agcgctacct
     4621 ttaattaagc cgaccgctcc gctcgtcggc accggtatgg aacacatcgt cgctcgcgac
     4681 tccggattag ccgtcatctc gactggcgaa ggtgtcgtct cctatgtcga cagccgcaga
     4741 atcgtcgtca aagacagcaa aaagaaaact aagacttatc aattacagaa gtttgcccgt
     4801 tccaatcaag ggacgtgcat taatcagatc gccgtcgtca aggtcggtca ggaagtcaag
     4861 accggcgata ttctcgccga cggacccgcc atgcaaaacg gcgacctcgc cctcggacaa
     4921 aatgtcacga tcgcattcat gacctggaat ggctataact atgaagatgc tgtcatcatg
     4981 tcagaaaggc tcgtcaaaga cgattcttac acctcgatcc atattgaaaa atatgaatgc
     5041 gagtgccgcg ataccaaatt aggcgccgaa gaaatcaccc gcgatgttcc caatatcagc
     5101 gaagaagcta aagctttcct tgatgaacgc ggcatcgtcg ttccgggcgc tgaagttaaa
     5161 gaaggcgata ttctcgtcgg caaaattact ccgaaaggcg tgaccgaacc gactcctgaa
     5221 gaaaagctat taatggcaat cttctctgaa aaaaccaaag aaggtaaaga tacctctctc
     5281 cgcgtttccc acggcggagc cggcatcgtc ctcgatgtca aaatcttcac ccgcaagaat
     5341 ggcgacgaac tccctccggg cgtcaacgaa gtcattcgcg tctatatcgt ccaaagacgt
     5401 aaaatttccg aaggcgataa gatgtcggga cgccacggca acaaaggcgt catctcgcgc
     5461 attttacctg tcgaagacat gcccttcttg cccgatggca caccggttga catcatgctc
     5521 aatccgctcg gcgttccctc gcgcatgaac atcggtcaaa tccttgaaat ccacctcggt
     5581 ttagcttgta agaaactcgg cttgaaacta gcgacgccgg tttttgacgg catcaccaac
     5641 gaggagatat tcgatttgat gaaaaaagcc gaaatcgctc ccgatggcaa aaccatcctc
     5701 tatgatggcc gcaccggcga gcgctttgat gagcgcattt ccgtcggcgt catgtacatg
     5761 attaaactcg tccacatggt cgatgataaa ttgcatgccc gttcgaccgg accttgttcc
     5821 ctcgtcaccc agcagccgct cggcggcaaa gcccaaaacg gcggtcagag attcggcgag
     5881 atggaagtct gggccctcga agcttatggc gccgctcaca ccctgcagga attactgacg
     5941 attaaatccg atgaccgcgt cggtcgcaac aagacctatg aagcgatcat caaaggatat
     6001 ccgattcccc gtcccggcat tcccgaagcc ttcaaagttc tcaccaaaga actgcaagcc
     6061 ctcggcatgg atgtcaaact gctcgataac gacaacaaca aaatcgatat ggatgctctg
     6121 gctaaagaga ttcagatcga agaaagaaaa tttaactcga ccattcgtac cttggcccgc
     6181 gatggtgtcg ccgacacctt gcccaccgaa ggcgatgatt acagcgagtt aattaactaa
     6241 ggaggccaga aacaatgttt gatgtaacta aattagccgc cctccaagtc ggactcgcct
     6301 ctcctgagac cattcgcacc tggtcgcatg gcgaggtatt aaaacccgaa acgatcaact
     6361 accggagcca aaaacccgaa gcgcaaggcc ttttctgtga aaagattttc ggcccgagca
     6421 aggattttga atgccactgt ggcaaataca agaaaaaacg ctttcagggc atcacatgcg
     6481 aaaagtgcgg tgtcgaagtc atctccaaag aagtgcgacg cgaacgcatg ggccatatcg
     6541 agctcgcctc gccctgcacc cacatctggt atctcaaagg cattcccagc cgcatcggct
     6601 tgattctcga tattcctccc aaacaacttg aggaaatcaa ctatttcgtc gcccacgtct
     6661 gtctcgacgc cggaacgagc aaggttttaa gttaccgcca attcctcgat gagcggaccg
     6721 cccgtcagga attcgttgcc gtcattcgcg atttccagcc gactttggat ccggaaagcg
     6781 ccgattactt aaagagcgaa gaattgatcg aaagaatgca aaacccgacc gaaaccttcg
     6841 atttcttcac ggttgccgac tttatttcga agtataccaa cgccgaattc ggcgaagggg
     6901 ccgccgcgat taagcgcctt ttaaaagaag tcaatctcga tgaagaattt aaaatcatca
     6961 acgccaggct caaagattgc agcgctcaaa accgcatcaa attatcgaag cgactcgaag
     7021 tcatcgaggc ctttcgcaac tctgataacc gtcccgaatg gatggtcctt gatgtcttgc
     7081 cggtcatccc tccggatctc cgcccgatgc ttcaactcga cggcggacgt ttcgcggcct
     7141 ccgatctcaa tgatctctat cgccgcgtca tcacccgcaa cacccgtttg aagaagctga
     7201 tcgacctcaa ttcaccttat gttattttaa tgaatgaaaa acgtatgctt caggaagcgg
     7261 tggacgcctt aatcgataac ggtcgccgca ataaaccggt tgtcggttcc ggcaatcgtc
     7321 cgcttaaatc gctatccagt tccttaaaag gaaaacaggg tcgcttccgt cagaacctgc
     7381 tcggcaaacg cgtcgactat tccggccgtt cagtcatcgc cgtcggaccc gatttaaaaa
     7441 tgtatcagtg cggacttccg cgcgagatgg ccgtccagct tttgcggccg ttcatcgcct
     7501 cgcagctgat gagagaaaaa cacgtcagca gcctcgtcca ggctcgcaag tacatcgacc
     7561 gctatgatcc cgtcgtctat gatgtcttgg aacggatcat cggcgatcac ccggttttat
     7621 taaaccgcgc cccgacgctg caccgcctcg gcattcaggc tttccagccg aaactcgtcg
     7681 atggccgcgc cattcgttta cacccattag tttgtaccgg ctttaacgcc gacttcgatg
     7741 gcgaccaaat ggccgtccat gttccgctca gtaaatccgc ccagcacgaa gccctcgaac
     7801 tcatgctcgc cagcaataat attctcggtc ccaaagatgg ccgtccgatc gtcacccctt
     7861 cgcaagatat ggtgctcggc aattactatc tgaccttaga agaaacaaaa gagcagttag
     7921 ccgctcgcag caaacgccgc cttgataaag gcgatatcga agaatatgag cgttttgccg
     7981 cctacgccaa cgccgaaggc aaagtcttta gaaatatcga tgaagcgatt ctcgcctatg
     8041 agacgcatca agttcacctc cacaaccgcg tggccattcg cgccagcgcc ctcgtcaaaa
     8101 ccggcttcac caaggaaatg aatgagtcct atctcatcac gaccgtcggc aagattatct
     8161 ttaacctcat cttcccggcc gatttcccct atttaaatga tgccagtact gagaacttga
     8221 aaaacgatct caaaaaattc tttgttccca aaggaacgga tatcagcgat tacattcatc
     8281 gccaacccct cgtcccgcct tttaccaaga agcatctcgg caatatcatc aatgaaatct
     8341 tcagccgcta cggcacttcg aagacctcag ccgtcctcga caagatgaaa gatatcggct
     8401 ttaagtatgc gaccatcgcc ggcgtgacga tttccatcag cgatatcaat gtcctcgata
     8461 ataaggaaaa aatcctcggt ggtggtgaag aaaaagtcga gcagatcaac gagatgttcg
     8521 aacatggtct cctcaccgaa aacgagcgtc acaaacatgt tgttgacgtg tggaccgatg
     8581 ttcgcgaccg cgttcagaaa gacctcgcca accagtttga aaaagactcc cggaatccga
     8641 tctttatgat gatggacagc ggtgctcgcg gcaacatctc caacttcact cagctcgccg
     8701 gcatgcgcgg tttgatgtct aaccctaagg gcgacaccat cgaacttccg attaaatcgt
     8761 gcttccgcga aggattaacc gtttccgaat tctttatcgc cacccacggc gcccgtaagg
     8821 gcggtgcgga tacggcgctt aagaccgccg actccggcta tctgacccgc cgcctcgtcg
     8881 acgtcagcca agacgtcatc gttcgcgaag atgattgcca cagcgatcac ggtcatgtcg
     8941 tctctgaaat tatcgattcg agccgcagca caatcatcgt tccgctttat gaccgactgg
     9001 tcggtcgtta tacggttcat aatgtcgttt cgcctttgac cggcgaagtt atcgtcgctg
     9061 agaatacgat gatcgatgaa gaatccgcca agcgaattgt cgatgcgggt atcaaagaag
     9121 tcgaaattcg ttcgctcttt ggctgtcaaa cgcgcgatgg cgtgtgctgt cactgctatg
     9181 gccgcaattt ggcgaccggc aaagaagtca caatcggcga agcggtcggc atcatggccg
     9241 cccagtccat cggcgaaccc ggcactcagc tcacgatgag aaccttccat accggcggcg
     9301 ttgccggcag cgatatcacc caaggtttgc cccgtgttca ggaactagtc gaagcccgcg
     9361 tccccaaagg cgaagctttg atttccgaaa tcaccggcgt cgtaaaccac atcgaagaat
     9421 ctggcggacg ctataccgtc actatcaaaa atgagctcga agagaaggaa tacacttcta
     9481 attacggagc tcgcctccgc gttaagaaag gcgataaaat ccgcaatggc ggcaagatca
     9541 ccgaaggtgc tatctcgccg aaaaaacttc ttgaagttag cgacatcgct gccgttcaac
     9601 gctacatttt aaaagagatt cagaaagttt accgcgctca gggtatcggc atctctgata
     9661 agcacatcga agtcatcatt cgccagatgt taagaaaagt cgccatcatc gaaggcggcg
     9721 acacgaacat gctgcccggc accctcgtcg agctcgacga attcaccgaa aagaacgagg
     9781 acgccctctt atccggccgt caccccgctt tggccagacc ggtcatcctc ggcatcacca
     9841 aggcatcatt gcaaaccaaa tcgttccttt cggccgcctc ctttcaggag acgacgcgcg
     9901 ttcttaccga tgcggcaatt aaagccaaaa cggatattct ccacggctta aaagaaaacg
     9961 tcatcaccgg caaactcatt ccggcgggac gcggtctcca ctccgacgaa gaagaagaga
    10021 cactcgtcaa gagtttctcg gtccaggaga agatgaagga agttaagcaa caatacatcg
    10081 aagtccacga tcgtcctgac gatttagact gagaaatgat taggcggtga taccgccttt
    10141 tcttttgata aacattattg attttttaat caaaagaatt gacaacctag actcgtttgt
    10201 tataattact acacttatta gacttaactg tgttaacgtt ttatcggact ttaccataat
    10261 tatctactta agcattcagg aggttaagaa tatgaaacct aacaaaatac cagcgattgc
    10321 catcttctcc gtttttatct tggctagttg cggacccacc gtggtagaag ataattttta
    10381 tcgttttaac gataaagaag cggttgccac ccttactcag gctcgtaaag ggacttatat
    10441 cagcgatatg ccgagatccg gtgaatcgcg cgttttggtc gtccctgtcg agttctccga
    10501 tttcccggct tccggtatgc tgagaggcgc cgaaggagcc cgcgaggatc ttcgcaaagc
    10561 gtattttggc gaatccgaag agacgcagtg ggaatcactg agcagctatt atgccaagtc
    10621 aagttacggc aaagtgaata ttactggtca ggtcatgccg tggtatcgtc ccaagaatcc
    10681 ttcaaaaccc agccagtggt attcgactga agaaatcatc agcagttatg gttcttccga
    10741 aagcaccggc atctcccgcc tcatcctagg ctggttttat accgattatg caatcgatat
    10801 gtatcaaaat gttttagatg atgagggcaa tccgtatgcg acggcggcag ctttctttaa
    10861 agattatgac ggtgacggcg atggttacat cgatgtcata cagatggtct acagcgctcc
    10921 cgtcaaccac ctatccagcg atttgttttg ggctttccgc tcagtcgact catcgtcggc
    10981 ccccaacaag aacagcccct ttccgtgcgc ttatgtctgg ctcggttacg atttcatcta
    11041 tgaaaatggt tactacgata atctaggcgt ctaccacgat tggaccgatg ctgaaatcat
    11101 cgccggaacc gccgaaatcg atgcccacac gatcattcac gaatccggac acgcccttgg
    11161 cgcggatgac tattacacct atgatcaaaa agattggggc gctttaggcg gtaccgacat
    11221 gatggactat aacatcggcg atcacaatgc ctacaccaaa ggtatctttg gctgggccga
    11281 cccgattgtc gtcaccggcg aagcgacggt caaagtcaaa tccttcaccg ataccggcga
    11341 agcgatcgtt ttaccggctt accgcgcaga cggcgaagtc agcaactcgc tgctcgacaa
    11401 ctacatcatg attgaatatt accggccgac cggacttaat gagctcgatt cgacttataa
    11461 ttacaccggc cgctatccca agatgcccga tgttcccggc attcgcgttt atcatgtcga
    11521 ctcgcgtctg ggcctcttca cttatggatc gacctcgggc tggcagtttg tccgttatgt
    11581 ttcaaccgtg acggcgaccg acagcaattc atacatcgat atcgccaact cgaacaccaa
    11641 gtcgcgtagc gccgatccga acaatcgcct cattcacgct cttgaagtaa gcggtgagaa
    11701 cactctgaaa aacacgactt ttaacacccg ttacagctcg agtatgatgt ggcaggaagg
    11761 cgacacgttc ggctacgata cgtttgtgaa tttcacgctt aataatggcg agaaactcgg
    11821 ttataaattc gaagttaccg acatgaacga cgaagacgtc accattaaat tcagcctcgc
    11881 ttaatctctt catcaaaaac aataaacctt tcggagaaat ccgaagggtt tttaattgta
    11941 tcgcattctg attgtatctt tttttcatca gtgctatctt tagataacta tgaaaaataa
    12001 agccttttct tttttcttcc taatcactct tctcaccgcc ggatgcgcgc cgaccgctga
    12061 ttcgtcttcc gcaccgagtt ttagcgagga tccggtttat gatgcttatt atcgcgcaga
    12121 aaatcaggag ttgacttact acgatgtttc gcgatccgga gggatggaca gccttccaac
    12181 cgtcggcgag gtcaatttac tcgtcgttcc ggtcatcatc gagggttatg aagaaaacgc
    12241 ttctaacgag acgcgaagca atattcaaaa agtgatgttt ggccaatcgg aagatacaag
    12301 ttgggaatcg gtcgccagtt tttatgataa atcaagctat ggtcgcttga cgcttaccgg
    12361 cgaggtgact ccctggtttc cttccggcta ttccgccgcc gagatgaatg ccatggaagc
    12421 gcgaggtcag gattccgtat ccctgcttct tgaagatgcg gtggattggt tgagggaaga
    12481 acaaacaacg gtcgacttaa ccgcttatga taacgaccgg gatggtcatc tcgatgcggt
    12541 gtggatgatc tactccgccc cttcgcaaat aagcgatgtc ttctgggctt tcgtcaattg
    12601 gaattatcgt aacttaaata acaaaaatat tgcaaatccc acccccttta ctcatgcctg
    12661 ggctagtttc gacttcatgt atgagggtta tggaacaagc ggaatcgacg cccacaccta
    12721 cattcacgaa accggtcact tgctcggtct cgatgactat tatgactacg acggcaagac
    12781 ttccccgatg gggatgatcg acatgatgga ctacaacatc atcgatcata acggctactc
    12841 aaaatttgcc ttagggtgga ctaaccccta tattgtcacc ggcgacgccg atatcaattt
    12901 gctgccggcg tcttcaagcg gtcagtcgat tcttttgacg gcggactgga acggcaatcc
    12961 ttttgatgaa tacttgctcc ttgaactcta tactcccgat ggattaaacg aaaaggatgc
    13021 gctgaacagt tatcccgcaa atggaaagcg cggtttcaca attccgggaa tccgcatgta
    13081 tcatgccgat gcccgccttt ttgattctga aaccaacaca tatgtcgacg acctaagcgc
    13141 gagcgaagcc tatgtcggag cctctaattc accatcctgg tcgtatttaa aagattataa
    13201 gagcaaattc aaattgctcc atgtcgtcga tgccgcgaaa aatattagat atatgagcag
    13261 ccaatatgcg cgggcgaatg acgatacact ttttacctcg gatatgacat ttagctttga
    13321 agaatacaaa acagcttttg cccgcggctc gaacttgctg atgaatgacg ggacgacttt
    13381 cccctttacg attgagtttt ctgaggtgag cgcgcaagga gcgacaataa aaataagagt
    13441 tatttgattt atatagtgag ataatgcact atttttctga ggatttatat gaaacgtaaa
    13501 atgttatatt tggcggttct atcggcgcta acgctaacca gttgcgcgct ttctttcggg
    13561 acgaccagca ctgacgattc gagcaccggc tcatccgata cgagcggcga tagcacctcg
    13621 tctgatacgg gcgctgatac ctcatcagga acaagttcgg gcacttccgg taatacatct
    13681 tcggatacaa cctcgagcac aggcggcgat acctcctcat ccgcttcgac cgcgactgag
    13741 ggctgggata attattttaa accagacatt gtcgatgaat atcactctta tcaagacttg
    13801 caaaaaaaca tctatctgaa ctcaattcct tctcaaggcg aagatattga tattttagtt
    13861 gttcccgtcg aattcaccga ttatccgttt gccacgaaga cgttggcgga tttagaaatt
    13921 ctttttaacg gtacatcttc gcagacgaat tattgggaat cggtcgcctc gttttatgag
    13981 acatcaagtt acggtcacct cgacttcgat tttacgattg ccgccaccta tgataccgaa
    14041 aagaccgttt cgcaattcgt atcgacttat ggcagtttct ttacgaccaa gcttgtaagt
    14101 aaagtcgtca gtgactacaa gaccgcgaac gggaattcat caacccagca atttgataac
    14161 gacagcgatg gtttcatcga cgccgtctgg atgatttata gttgtccaaa ttatagcaat
    14221 tctgattata tcgcgaatat ttccgagaat ttctgggcct atgtgacatg ggatgcccag
    14281 cagtcatata acggaagcgg atcagtttcc agcccggtcg ccaatgtcta cggatgggcc
    14341 agctatgatt tcatgtatga aggcggcggt gatacaaaaa tcgacgccca cacctacata
    14401 catgaaacag ggcacttgct cggccttgat gactattaca attatgatga agacagcgat
    14461 tataagcctt taggcgcgat cgatatgatg gattataaca tcatcgatca caatgcttgg
    14521 tcaaagatgg ctttgggatg gctcaaacca tatgtggtga ccggcgatgc cgaaatcacc
    14581 atcaatcccg tcgagagcag cggtgacgcc atcttgatcg ccgataattg gaacggaact
    14641 tcgtttgacg aattcatcct gcttgaacta tacactccca caggattaaa taaactcgat
    14701 tcgagaacaa attacgtcgg cagataccct cgagcctaca cgacggccgg agtccgtttg
    14761 ttgcacattg acgcgcgcct cggaattttt aattattcga atcagtttat caattacggc
    14821 gatcccggtt cgggccctct ttataatgat tcgacgcaga gatattttgc gatggcaaat
    14881 tccaacaccc catcctatag cgccgacgaa aatcatcgcc tcgttcatat gattcaagcc
    14941 ctcggcacga atacgtttga cgaaggaatg agtggcacca attctgacct tttcaaaacc
    15001 agtcagactt tttcgatgtc gacccacgga accgagttct ttaaaaacgg aaacaaatta
    15061 aacaacggga atgcattagg ctacgccatt tatttctcaa gcgtcagctc ggaatccgca
    15121 acgataagaa tcgaaaaaat atagtattat atattaaatg aaaccaaaaa agatgttccg
    15181 ttccctcctg attccggtct ttggcatctc agccatggtt ctttcatcgt gcacgagcgt
    15241 ctattttgct tttattcctt ttcgcgatta ccaagttgat agcgatgacc atgaaacgat
    15301 tattgaagat ggctattata agcccggaga ctactcgcca tatacatact taaacggcga
    15361 tggcgaagag gagtcttttg ccaattttac cgatgtctac cggcacaagg attatcgccg
    15421 gagcatgaga tcgctcggcc ggcagaaaat actcgtcatc cccgtcgatt ttgaagacta
    15481 tccttcttct tcgcttccgg aaggaaccga gggatctttg gaagttctac gcaatgcctt
    15541 ttttggtatt aatcaaaata atcaatggcg ttcagtcgcc ggatactata acgaatctag
    15601 ttacgggaag ttggttctcg atggcaaggt gaccgactgg tatcgctctt caaaactgac
    15661 cgccgatatc cgcaactcaa gcgcgacgag tcaaatcgtc cgcgatattt ataatggtgc
    15721 cttggcgtgg tatcaggaaa catatgggga tttagcgagc tattatgtcg atggcgaccc
    15781 gagtaaatca gtccctgtct acctcatcta tacacatccc tctgagacag gatcaggagc
    15841 ccgagacaag ttattttggg cctttacaat caatcagaag ccgacgctga catgctggtc
    15901 gagctattct ttaacttatc tgagcgaagg gcgacccgac acccatactt acattcatga
    15961 agtcggtcat cttctcggcc tcgaagacta ttacaacacc gacagcgaac tctatggccc
    16021 gaccggtcgc gcggacatga tggattacag catcggcgat cacaccggtt attcgaagat
    16081 gctgctcgac tggacgcgac cctatgtcgt caccgacacc accgagatca cgcttcggcc
    16141 cttttataac agcggagact taatattaat taaagacaac tggaacggga cggcgatgga
    16201 cgaatatatg ctgcttgaat tttattcgcc caatggctta aacgcccacg attcgcgctt
    16261 gactaattcc aatccgcaac tcatgcaaca tgcgggtatc aaggtctatc atgtcgatgc
    16321 tcgtctggct tattattcaa acgatggttt tcggcgggct ttagggtatg tcgaagacgg
    16381 cgtctacagc caggagacaa accgcgtcgg tatcgcgcac agcaatacgc agtcaacgac
    16441 gtacaataac taccggcttt atcatctttt ggagagcggc ggagaaaaca ctttcattaa
    16501 cggtggaacg gctacgaata agacgctttt taaaaaaggc gattcgttcg gcgtcgagac
    16561 ctttgctgat tttaccttta acggtggcca ggaattaggc tatactttca cgatttccga
    16621 tctcgccaac acgtacgcta agatatctat caccaaaatt taactttttt tcttgacgaa
    16681 tatcgctctg agttgttatt atctttcagg acacatattg ggccgcatag gcctatttat
    16741 ttgtgagatg tatgatacac cgggaaatgt gtacgtgcat cgatatataa ggaggatgtc
    16801 caatgccaac cattaatcag ctagtcaggc agggacgcgt tcgttcgaca ttcaaatcaa
    16861 aagccccagc tttaggaaag agatggaact cgttacaaaa acgccagacc tcgcaaaatt
    16921 ccgcccagaa gcgcggcgtt tgcacccgtg tcggaacgat gacgccgaaa aaacctaact
    16981 cagccctcag aaagtacgct cgtgtgcgtt tgtcaaacag ctacgaagtg accgcttata
    17041 tcggcggtga aggtcacaac cttcaagagc actccacggt tttaatccgc ggcggtcgcg
    17101 tcaaagattt accaggtgtt cgttaccaca tcattcgcgg cactctggat acggccggta
    17161 tcgaatcgcg ccgtcagggc cgcagtcagt acggcaccaa aagacctaag tagttttagg
    17221 taaacaaaag gagataaaaa tatgccacgc aaaggaagtg tgagcaaacg cgatgtcttg
    17281 cccgatccaa tttacagttc aaaattggtc acgcgtttga tcaacaaaat aatgctcgac
    17341 ggcaaaagag gaaccgctca gacgattctt tatagcgcct tcaaaaaaat tgaaacgaaa
    17401 accggcaaac ccgccatgga tgttttcgcc acggcgatca ataacatcat gcccgaagtc
    17461 cagcttaaag tccgccgcat cggagccgcc aactatcaag ttccgaccga agtcacggct
    17521 gatcgcaaag tcactttagg tttacgttgg ttggtgacct acgcccgtct tcgcaacgaa
    17581 aagacgatgg aagataagct cgctaacgaa atcatcgatg ccgccaacgg caccggtgct
    17641 tccgtcaaga aaagagaaga tacccataag atggccgaag cgaataaggc gtactctcat
    17701 taccgttggt aacgagggag tcttattatg gcacgtgaat atgacttaga acacacccgc
    17761 aacatcggca tcatggccca cattgatgcg ggtaagacga cgacgacaga acgaattctt
    17821 tactataccg ggaaaattca taaaatcggc gagacccacg agggttcagc gacgatggac
    17881 tggatggccc aggaacagga gcgcggcatc acgattacat cggcggccac gaccgctttc
    17941 tggaaagggc accgcgtcaa catcatcgac actcccgggc acgtcgactt cactgttgaa
    18001 gtagaacgct cgctccgcgt attagatggt gcggtgaccg tcctcgacgg caaaaacggc
    18061 gttgagccgc aaaccgaaac cgtctggcgc cagggcacca aatacaatgt tccgcgcatt
    18121 gtcttcgtca acaaactaga cgcgatcggc gccgatttcg atatgtgcat cgaaacactg
    18181 cacactcgcc ttgcggcaaa tgccgaagcc gttcagtatc cgattggcat cgagagccgt
    18241 tttaacggca tcatcgattt aatcaaccgc aaagcctata cctattcagc ggataaaagc
    18301 gagggaccga cggaaatcgc catccccgaa gaatatctcg agaaggtcga agcgatgcgc
    18361 gctaaactcc tggaagccct cgcggctttt gatgatgaac tgatgctcat catcctcgaa
    18421 ggacaagaac cttcaatcga tcttattaaa accacaattc gcaaagctgt tttaggcggt
    18481 gaattcttcc ccgtcttctg cggttcggca tataaaaaca aaggcgttca attgctcctc
    18541 gacggcgtta ttgattacct gccgagccca attgacatcc cccatcagaa aggtcattac
    18601 agcgatggcc gcgaatacac ctgcgaacca gatgataagc aaccgttagc tgctttggcg
    18661 tttaaaatcg ccaccgatcc cttcatcggc cgcctcgcct atgtgcgcat ttattcaggg
    18721 accctcaaaa gcggttccta tgtcatcaat accaccaaag gaatcaagga acgcatcggc
    18781 cgcgtcgtct tgatgcactc caatcaccgc caggaagtcg aagaactcca cgccggtgac
    18841 atcggagccg tcgtcggact gaaaaatacg acgaccggcg acacgctttg cgaagaaaaa
    18901 ctcgatgtca tgctcgagaa gatggaattc cctgaacccg tcatcgaaca ggcggtcgag
    18961 ccaaaatcga aagccgatca ggaaaaaatg ggcgtggctt tacaaaaact ctctgaagaa
    19021 gatccgacct tccgcgttca cacaaacagc gaaaccggcc agacgatcat cgccggcgtc
    19081 ggtgaactgc atctcgatat catcgtcgac cgcatgaagc gcgaattcgg cgttcaagtc
    19141 aatgtcggag ccccgcaagt cggctatcgc gagacgatta aaggcagcgg cgaatgcgaa
    19201 ggcaaataca tcaagcaatc cggtggtcgc ggtcagtacg gccacgtcaa aatccgcttt
    19261 gaaccgaatc ccggcaaagg cttcgaattc gtcgacaata tcgtcggcgg tgtcgtcccg
    19321 aaagaataca tccgcccgac aaatgatggt ctgcgcgatt cgctcgaaag aggtttggtc
    19381 gccggctatc ccgtcatcga tatcaaagcg acattattcg atggtagcta ccacgaagtc
    19441 gacagcagcg aagcggccta taaaatcgcc gccagcatgg cacttaagga agccgccaga
    19501 acatgtaatc ccgtcgtcct cgaaccgatc atgaaagtgg aagtgacagt ccccgaggat
    19561 tattacggcg atgtcatcgg tgacatcacg cgccgccgcg gtcaaatgac cggcgaatcg
    19621 cagcgcggta acgccaagac cgtcgaagcc gatgtccctc tctccgagat gtttggttac
    19681 gtgacggatc tgcgctcctt cactcagggt cgcggcaact acacgatgca gttctcccac
    19741 tatggagaat gcccgcgttt catccaggaa gaaatcatca aaaaatccgg gatgagccaa
    19801 cgctaataaa tagatacgtt gtattgattt tacaagaagt ttgctataat atgaattcgg
    19861 ttgaatttat caaatgaaat aaggaggaaa ttcaaacaat ggcaaaagaa aaatttaata
    19921 gagcgaagac gcacgtcaat gttggcacga ttggtcacgt cgaccacggc aaaacgacct
    19981 taacggccgc cattacgaaa gtcttagcaa aaagcggtgg agccaaggca acagcctacg
    20041 cgcaaatcga ctccgctccg gaagaaaaag ctcgtggtat cacgattaac acttcccaca
    20101 tcgaatactc gacagccaaa cggcactatg cccacgtcga ctgcccaggg cacgctgact
    20161 atgtcaaaaa catgattacc ggcgccgccc agatggatgg cgcgattctc gtcgtcgccg
    20221 ccaccgatgg cgtcatgtcg caaacccgtg aacacatttt gctcgcccgt caagtcggtg
    20281 tcccttacat cgtcgtcttc ttaaacaaga tcgaccttgt cgatgacccc gaactcgtcg
    20341 atctcgtcga gatggacgtt cgcgatcttc tctcatctta cggattcccg ggagacactc
    20401 tccccgtcat ccgcggctca gcccgcgatg cccttgaagg cgatgacgaa gcttgcattc
    20461 ttgaactcat ggatgccgtc gacagctaca ttcccgatcc cgttcgcgat aaggataaac
    20521 cgttcatgct tccgatcgaa gacgtcctga ccatcaccgg tcgcggcacc gtcgtcaccg
    20581 gcagagttga ccgtggctta ttaaaactca acgacgaagt tgaaatcatc ggtatcaaac
    20641 cgacgattaa gtccgtcgtc accggcatcg aaatgttccg caaattgctc gacttcgccg
    20701 aacctggcga taacgtcggc gtcttactcc gcggcatcaa ccgcgatcaa gtcgtccgcg
    20761 gccaagttct cgccaagccg ggaagcgtca ccccgcatac caaatttacc gccagcacgt
    20821 atgtcttaaa gaaggaagaa ggcggccgtc acacggcttt cctgagcaac taccgcccgc
    20881 aattctactt ccgcacgact gatatcaccg gtgtcatcac ccttccggcc ggcgtcgaaa
    20941 tggtcatgcc tggcgataa
//
LOCUS       DAOSSL010000018        18199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_3222_length_18199_cov_14.288635, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000018 DAOSSL010000000
VERSION     DAOSSL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18199
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_18199_cov_14.288635"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1119)
                     /locus_tag="PLH17_02805"
     CDS             complement(10..1119)
                     /locus_tag="PLH17_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20742.1"
                     /translation="MKRLGLVITALSITASLLAGCDVSKNKTPLLFGTLEDELVALSY
                     EQYVEKIEDKDNFLVFSTPNSLCTCWTSFRDSILMPYRKANNLIVYTVHFSEFFDDDN
                     AKRDSYGLDFISSSQTLGLFKDGVIKENRPYNSTHKIWGSKDSFATYMKELIVFPTII
                     DINLTKLTNLYAQDSSFTVIYYDDETESTYLRENFLESYARENAATMNNLYLLDTDVD
                     GIKLKSGVYDENQWQGFKDTYGLSALNNATLGYGTGFVPTAQYVEPNGIDTNGSIIKA
                     QSVYLNDVLTISANPAVYEVTESFYSQERENSLSYLTDFVGDPILVAKEVPAADVDID
                     GANVTWKHEKAALYHDPLIRAFLDYSLPLATSVLE"
     gene            1508..3022
                     /locus_tag="PLH17_02810"
     CDS             1508..3022
                     /locus_tag="PLH17_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20743.1"
                     /translation="MSKNETIISLKNVSKQFDGTLVVDAFNLDIKRGEFVTILGPSGC
                     GKTTTLRMIAGFETPSSGEILLNGTDISVLPPYKRPVNTVFQRYALFPHLDVYDNIAF
                     GLKLKKINETYTDKNGEEKVRCRHLSIEEIDEKVAKALKMVDLEELEDRDVQTLSGGQ
                     QQRVAIARCIVNEPKILLLDEPLGALDLKMRKDMQLELKAMHDKLGITFIYVTHDQEE
                     ALTMSDTIVVMKDGVIQQVGTPMDIYNEPANAFVADFIGNSNIYNATMVGPKVVRFIN
                     ANFPCDDDYPLNEKVDVVIRPEDVEIVASGKGMVEAKIVNKIFKGVHFEYVVMVGKNE
                     VVVQSTKDFPEAEPIALIIDSESIHVMKKEFTINSFYDAWINKNNQVVIGNIAFDCDV
                     TKLVPQSTLDQDGYVVSKKGVRYDFNDAEVVAEIQLSDIELSDDISQGQTTGTVVSML
                     YIGDHYQYTVRTEEEEDFVVDTQYLFNENDVVSINVAPQHILLRVKGDIAKYEL"
     gene            3012..3806
                     /locus_tag="PLH17_02815"
     CDS             3012..3806
                     /locus_tag="PLH17_02815"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20744.1"
                     /translation="MNFKRKYLAIPYSLFLALFVIIPILLIVYYAFSNADGQISFDSL
                     VRFFSSTTKLNVLLVSFFIGILNTLLCLIIGYPLAYFLARKQYNTNTVLVLLFIMPMW
                     INFVLRTGATRDLLTWFGINGGQYPYLATMIGMVYNFLPFTILPLYSTMLKLDQSQIE
                     AAADLGANPFQVFTKSILPQSLPGIVSASTMVFMPTMSSYVISDVLSEGKITLFGNSI
                     YLNFSSSQWNDGSFMALIMLAIVGVTLLISRRFNKDDFASRGSNLW"
     gene            3800..4663
                     /locus_tag="PLH17_02820"
     CDS             3800..4663
                     /locus_tag="PLH17_02820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20745.1"
                     /translation="MVKSKKLFASIYVYLILFLMYLPILVLIAFSFTEATNIGVWTGF
                     SLNLYARLFQSQEIMVALGNTLIIAFISAAISTLLGTAGAIGAFYSRKRTLKAIEGVT
                     QIPIVNAEIVMALSLTVLFVFVGSVIFRGQSIFGFWTLLIGHVVLSVPFVYISVRPKL
                     MQLDPHLYEAALDLGATPQKALWKVIIPQIMPGILSGFLLSLTLSLDDFIITAFTRGT
                     GLLNGEGTIETLSTLVQAKIKRGPIPPEMRALTLIIFIVVLLVVVGITIYRNKTKNVK
                     KIRKGRENHEF"
     gene            4653..6245
                     /locus_tag="PLH17_02825"
     CDS             4653..6245
                     /locus_tag="PLH17_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR20746.1"
                     /translation="MNFKKTLAGTSAVLFSALLIAACSGNQPHEGGSQVLRILNWEDY
                     IYLNDPENGYEAKNLVDQFEDFIMDTEGIEVDVVYDTFDTNETMLNSLQTGKSVYDLV
                     CPSDYMIQKMIANDMLEPLDDAAANIPNYETFASPYLKSIFDNLEATNGVTQEVHSVS
                     EYAVGYMWGTFGILYNPTYATFADRGLSSDDVMSDMEDWNSLWNVDYKDTISVKDSMR
                     DTYSVGIMRVYDEEIRTLQDTYLDEAKAEYDPALYNAQLTDIFNRSDAETVAAVKEEL
                     LKLKENIYGFENDSGKQDIVTGKIGINLAWSGDAVYAMDQADEETETELYYNMPETGG
                     NIWFDGWVMPKSDSLNKELAQKFLNFVSDPVNAAQNMDYIGYSSFIGGEDINTLVHEW
                     YDVREDEWETPIDEWKLANDITDDYIAYDLTYFFGDTVDTETIIYIEEDSVGRQLYAQ
                     YPEADMIPRLSLMSDFGDNNRYILAMWEEFKASSLPGWAIALIVVGVLAVAGVGGFFL
                     YRKNLAKKNRSARRQQAASSRR"
     gene            6311..6384
                     /locus_tag="PLH17_02830"
     tRNA            6311..6384
                     /locus_tag="PLH17_02830"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6343..6345,aa:Cys,seq:gca)
     gene            6453..6529
                     /locus_tag="PLH17_02835"
     tRNA            6453..6529
                     /locus_tag="PLH17_02835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6487..6489,aa:Arg,seq:acg)
     gene            6534..6610
                     /locus_tag="PLH17_02840"
     tRNA            6534..6610
                     /locus_tag="PLH17_02840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6568..6570,aa:Pro,seq:tgg)
     gene            6641..6714
                     /locus_tag="PLH17_02845"
     tRNA            6641..6714
                     /locus_tag="PLH17_02845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6673..6675,aa:Ala,seq:tgc)
     gene            6723..6799
                     /locus_tag="PLH17_02850"
     tRNA            6723..6799
                     /locus_tag="PLH17_02850"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6757..6759,aa:Met,seq:cat)
     gene            6805..6880
                     /locus_tag="PLH17_02855"
     tRNA            6805..6880
                     /locus_tag="PLH17_02855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6838..6840,aa:Ile,seq:cat)
     gene            6886..6976
                     /locus_tag="PLH17_02860"
     tRNA            6886..6976
                     /locus_tag="PLH17_02860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6920..6922,aa:Ser,seq:tga)
     gene            7094..7432
                     /locus_tag="PLH17_02865"
     CDS             7094..7432
                     /locus_tag="PLH17_02865"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOR20747.1"
                     /translation="MAKINDEHFIQLMKILSDTTRFAIVRLLAQKGSLCACKILDCLH
                     ISQGTLSHHMKVLTNAKIVSCSKDGKWRHYRLLPHSLYKLSTFIQEITLIPSETTEEN
                     CACSIDVIGK"
     gene            7570..8547
                     /locus_tag="PLH17_02870"
     CDS             7570..8547
                     /locus_tag="PLH17_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOR20748.1"
                     /translation="MKWLNDLIWKIVQAVSGVHSLETWQYKVQSSIQFFVYDIIKITV
                     LLCFLIFVISYIQSYFPPERTKKILSKYHGIGANIIGALLGTVTPFCSCSSIPIFIGF
                     TRAGLSTGVTFSFLISSPLVDLGSLMLLVGVFGWPIAIAYVVAGLIISVIGGTIIEKT
                     GLGKHVEDFVSKGESVEIESPELTRKDRIIYARDQMWGTFKKVFPYMLIGVAIGAAIH
                     NVIPREWIENILGSEKWYGVPLAALLGAPMYADIFGTLPVAEALYNKGAGLGTVLTFM
                     MSVTALSLPSLIMLRKAVKPKLLIAFLIIVLIGIIAIGYLFNLFGYFFI"
     gene            8558..8899
                     /locus_tag="PLH17_02875"
     CDS             8558..8899
                     /locus_tag="PLH17_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR20749.1"
                     /translation="MKIQALGGCCKKSTSNYEAIVQAVKELDLKVEVEHVTDFGEIMK
                     LGVMSTPGLAINGKILSVGRALNVMQAKELINKVMKSESECSCGERCDCGDNCECEDG
                     CDCADDPSCCK"
     gene            8968..9990
                     /locus_tag="PLH17_02880"
     CDS             8968..9990
                     /locus_tag="PLH17_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOR20750.1"
                     /translation="MQHLLDQGPLLNEEGNLTESGYAFSLIKKYSRDAIKAPAMRIKE
                     WDYYYVGNNHVGVALTVADNAYMSLVGATVFNFDKAYDKTKNVMGLLPKGKIMMPSSS
                     ASGDVIFNTKNFSFHFTHENGRRHLVCEMKNFDGEKPFRCDIYLEETIKDSMVIATPF
                     NKPKHFYYNQKINLLKANGYAKVGDETYDLSKDTWGVLDWGRGVWTYSNTWYWSSMSG
                     TYKGKKIGFNLGYGFGDTSAASENMFFYDDKAYKFEDVKFDIPIASNGNDDFLKPWKF
                     RSKTGDISLTFEPILDRFSNTNVLIIKSIQHQVFGKFHGYFVIEGKQVYFEDILGFAE
                     KVRNCW"
     gene            10063..>11126
                     /locus_tag="PLH17_02885"
     CDS             10063..>11126
                     /locus_tag="PLH17_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20751.1"
                     /translation="MKINSTFTGEIVPRATKWIYSATAMGRDAAYTLISLFLIAYIQF
                     AAPIGYVNDVYSRGDYFAQFGVISIIVIIARIWDGLNDPIMGFIIERCHFKMGKYKPW
                     ILIGAITNTAVMLVLFLARPQGWAYVALFGVFYFLWDITYTMNDIGYWSMLPALTSDE
                     KQRNEITTIMSIFISIGAFAVGGVVPLTVANNAVQNYGIIAIVVSVVFLLSQLILVFF
                     AKESARNLEQEKISQKTKFGDMFRLMKTNDQLRWIVIVILIYYIASNILNGLGLNYFY
                     FMFGYGNDAGEGGNIQFLFTIMYAGGTILAQFLFPLLIKFLKRSTLLLISFLMMALGH
                     VVFFFIGAPFNGVPALGVQYP"
     assembly_gap    11127..11226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11227..11621
                     /locus_tag="PLH17_02890"
     CDS             <11227..11621
                     /locus_tag="PLH17_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20752.1"
                     /translation="GERKEAVAFSLRPLTAKLASSAQQGIVWVTLSVSGALAVTEGIS
                     KLEVDKGLGIATDIETGANQLIANGNQDMIIFAAVMTLLPFLLYVGAYLITRFLYNID
                     EKKYAQIVKEVDERRASLAVETPQEEVK"
     gene            complement(11664..13991)
                     /locus_tag="PLH17_02895"
     CDS             complement(11664..13991)
                     /locus_tag="PLH17_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOR20753.1"
                     /translation="MKQPLNFAWRYMPCYKQSFLNAFPEEAEKIDLPHSNVELPRAYF
                     DEDIYQFISSYEKVFDIETSLKNKTVLLTFHGVMLKAKIYLNHAFLGEYVSGYLPFTI
                     DVSGIAKAKDNRLVVIVDSHEDNSIPPFGGVIDYLTFGGIYREVELEIKPNIYLERVL
                     VTADRQGMLKINPHLGGNHSLNYEISHKLLFKDEVIKEFSENELQIENPHLWNLDTPH
                     LYTLLTTLKTTSGDDLVVTRFGFRSIRFEKSGFYLNDKRIKLIGLNRHQSYPYVGYAM
                     PKGAQEDDADILKYRLGVNIVRTSHYPQSEHFLNRCDEIGLLVIGEIPGWQYVGKDET
                     WRNHFLDFVKRMILKEHNHPSLIANGVRINESQDDHELYAASNSLARELDPGRPTLGV
                     RNFKNSELLEDIYAFNDFSSEDLSHGLDNPKTVEAGKAPVLVTEYLGHMYPTKTLDSM
                     ERRIHHALRHAKVIDDNFAYGNLIGAIGWCFADYYTHRDFGSGDHVCHHGVLDMFRNP
                     KYAASIYASQSDDNFVFEVLSNLTPGDFSEAIVGVTHVATNADYIELYRNDVFVKAFY
                     PNRAKYRHMPHPPIEVDDYIGASFSEAKFNSRDAERIKDALAYVALHGLNKLPFKHKI
                     RVGLVMFKHRMSYDELVDLWNKYVGMWGAKLMVFKFKAYKDGKLVASKMFGQSTEFSL
                     KARANKNVLLNADTYDVARVEIGLFDQYETLCNYSTLPLFIKTRGPIQIIGPSIITLN
                     GGRISVYVRSLKQRGKAALEISGEEYGAKVEFEVK"
     gene            14057..16390
                     /locus_tag="PLH17_02900"
     CDS             14057..16390
                     /locus_tag="PLH17_02900"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOR20754.1"
                     /translation="MITCKETLFHIETSKTSYVLRVNEINHIVNEYYGARLKNLDDSS
                     AIIEKWPYAYGSSVIYDEDKNKALSLDLLSLETSCVGKGDYREPSVKIRSADGYVFDF
                     TYKHHEVRKDIQPLENLPSPREADEELIIFLSDDYAKVALELHYLVFADSDVIARNVV
                     LINNSESPLYLERLMSMQLDLINRDFSLLNLYGGWISEAQKTIRKVTPGIYVNDSKTG
                     ASSNRHNPFFMLMEDKANLNSGGVYAFNLMYSGNHQELVELSNYGKVRVQIGINPYCF
                     DFKVKQGEAFVTPYAVMTYGDRGLNSASQNMHSFVNHHVIKKAFEMMERPILLNNWEA
                     TYFKFTETKLLSLARAAKKIGIELFVLDDGWFKNRDDDTKALGDYELNKKKLPRGLGT
                     LAKKINDLGLKFGLWFEPEMVSEDSDLFRAHPDWIIGNRERGISKGRHQLVLDLSRKE
                     VCDYIVDSLDEVLGSANIEYVKWDYNRHISDFFSSKTKAGELLHRQVLGLYDVLKRIT
                     DKYPHVLFEGCSSGGNRFDLGILAYMPQTWASDDTDAYERIHIQSGYSLAYPLSSIGA
                     HVSASPSHQLLRNTPIDTRFNVASFGVLGYELDLSHLDEVETRAVKAQISFYKKHRRL
                     LQYGAFYQMQLIDDENAAVWMVLSEDKKEGIIGYFNGLQKPNPKTTILQGIDFLEDER
                     YAMNVRPQDHNIKKFGGLINMVLPIRVNENGFLVNQIAHHKVMPGEKESYVVTGAMIN
                     HGAIKLNPEWAGVGYDERVRVLGDFGSRLYHFEIIGK"
     gene            complement(16377..17066)
                     /locus_tag="PLH17_02905"
     CDS             complement(16377..17066)
                     /locus_tag="PLH17_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOR20755.1"
                     /translation="MEIAFYVLIGVFGLASFIHLIFCLLEKEAWRKATKPLTTLLLTG
                     AILILTLTYPEMPFPKYAIWLAGIMCVFGDLFALRIKSRPLFITGSIFYTVGHMLNAY
                     VMVSMLSYTMPWWIYVAIAGASLAFSGALYPLSRLLFGKIAYIINLYLSLHLINIALA
                     ILLLVDGKPIIATMILVGYALYFLSDFLLVYSAYGKDMRRRDFYIMLLFYASQLLIML
                     GLANTLLIISQ"
     gene            17173..17249
                     /locus_tag="PLH17_02910"
     tRNA            17173..17249
                     /locus_tag="PLH17_02910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17207..17209,aa:Met,seq:cat)
     gene            complement(17319..18071)
                     /locus_tag="PLH17_02915"
     CDS             complement(17319..18071)
                     /locus_tag="PLH17_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20756.1"
                     /translation="MHKNVGFYKEEEIVLMLNEKKVCQLSNNAKYIIREIFEHPDEEA
                     VVYAKRLDEFIKPDFYIRIGKNTHYISMKSGSSNTLHQEYVKEFCIYLRERGISNRTL
                     QTILLYQFGDGTLDGTGKERFPYEKLRFLLADRLKEANLELNRDKEFVISIINRVVFK
                     GAREENIEADYVYHGDKDYGILVSKKQMIKHCHRRDWAFIDSLHIGPILLRPHARYVG
                     KEIKREKSRQRLEFYWPNYYADLDYISKRYDG"
BASE COUNT         4890 a         4027 c         4127 g         5055 t
ORIGIN      
        1 cactttttct tattctagaa cgcttgtcgc aagcggcagg ctgtaatcaa gaaacgctct
       61 tatgagcgga tcatgataaa gggcggcttt ctcgtgcttc caagtcacat ttgccccatc
      121 gatatcaaca tcggcggcag gaacttcttt agcgactaaa attggatcgc cgacaaaatc
      181 ggttaagtat gaaagagaat tttctctttc ctgagaataa aaactttcgg tcacttcata
      241 tacggcggga ttcgcgctta tcgtcagcac atcgttaaga taaaccgatt gtgctttgat
      301 aatcgacccg ttcgtgtcaa tcccgttggg ctcgacatat tgggcggtcg ggacaaagcc
      361 agtcccgtag ccaagagtgg cgttattaag cgcggagaga ccataggtgt ctttaaagcc
      421 ttgccattgg ttttcatcat agacaccact tttaagtttg atgccgtcaa catccgtgtc
      481 taatagataa agattattca ttgtggctgc gttttcgcgc gcatagcttt caagaaaatt
      541 ttcgcgaaga tatgtcgatt ccgtctcatc atcataataa atgacggtaa aactcgaatc
      601 ttgggcatat aagttcgtaa gcttagtgag gttgatgtcg atgatcgtcg gaaaaacgat
      661 caactctttc atataagtcg caaatgagtc cttgctgccc caaatcttgt gggttgagtt
      721 gtatgggcgg ttttctttga tgacgccgtc tttaaaaagg cctaaggttt gtgatgaact
      781 gataaaatca aggccatacg aatcgcgctt ggcgttatca tcgtcaaaga attcggaaaa
      841 atgcacggtg tagacgatga gattattggc tttgcggtaa ggcatcagga ttgaatcgcg
      901 gaaactcgtc cagcaggtgc agagcgaatt cggcgtcgaa aataccaaaa aattatcttt
      961 gtcttctatt ttttcgacgt actgctcata acttaaagcg acaagttcat cttcgagcgt
     1021 cccaaacagg agcggagttt tgttcttgct tacatcgcag ccggcaagta gcgaagcggt
     1081 tatgctcaag gcggtgatta ctaatccaag gcgtttcata aatgtccctc gagataatat
     1141 tatattcatc gcttgctaaa tatgcgacaa ataacatttt tcgatttttg aaagtgcgtg
     1201 atacggagcc cggaaaaatg ccgtaaaaaa gtcagaaaaa aaggagaaag acttcttttg
     1261 aatttatgcg accgaaaaca atttcaaaaa aggtgcggaa aaaaacgctt actaagttaa
     1321 acaaatattg cgatgtcgtc gaataaattt gcagaaaccg aactttcaaa aaacagggtg
     1381 aaaatatttt cattttttct cgattaaaat ttgttgtatt ttcacgcgat ttcttctata
     1441 ttttattcgt gaaaattttc gggcccgttc gccttgaaat aaacacggct agcgaggtga
     1501 acaatcaatg tctaaaaatg aaactattat cagcctaaaa aatgtttcca agcaatttga
     1561 cggcacgctc gtcgtcgatg cttttaatct cgacatcaag cgcggagaat ttgtgacgat
     1621 cctcgggcca tccggctgcg gaaaaacgac aaccttgcgc atgattgccg gctttgagac
     1681 gccctcaagc ggagaaatat tgctcaacgg cacggatatt tccgtacttc ccccttataa
     1741 acgtccggtc aatactgtct ttcaacgcta tgccctcttt ccccaccttg atgtctacga
     1801 taatatcgcc tttggtttaa aacttaaaaa gattaacgag acctacaccg ataaaaacgg
     1861 cgaagagaaa gtccgatgcc gtcatctgag tattgaggaa atcgacgaaa aagtcgccaa
     1921 agccttaaag atggtcgacc tggaagaact cgaagaccgc gatgtgcaga cgctttccgg
     1981 cggccagcag caaagagtgg ccatcgcccg ttgcatcgtc aatgaaccaa agattttatt
     2041 actcgatgag cccttaggag ctctcgatct caagatgcgc aaagatatgc aactcgaact
     2101 taaggccatg catgataaat tgggcatcac ttttatctat gtcacccacg accaagagga
     2161 agccctgacg atgtccgata cgatcgtcgt catgaaagac ggcgtcattc agcaggtcgg
     2221 tacaccgatg gacatctata atgaacccgc caatgctttt gtcgccgact tcatcggaaa
     2281 ttcgaatatt tacaatgcga cgatggtcgg accaaaagtc gtgcgcttta tcaatgccaa
     2341 cttcccgtgc gatgacgatt atccgctcaa cgaaaaagtc gatgttgtca tccgccccga
     2401 agacgtcgaa atcgtcgcca gcggcaaagg catggtcgaa gccaaaatcg tcaataaaat
     2461 ttttaaaggc gtgcattttg agtatgtcgt gatggtcgga aagaatgaag ttgtcgtgca
     2521 aagcacgaag gactttcccg aggccgaacc gatcgccctt atcatcgatt cggaaagcat
     2581 ccatgtcatg aagaaagaat tcacgatcaa cagtttctat gacgcctgga tcaacaaaaa
     2641 caatcaagtc gtcatcggca atatcgcttt tgattgcgat gtgacaaaac tcgtccctca
     2701 atcaacgctt gatcaagatg gctatgtcgt cagcaaaaaa ggcgtgcgtt acgactttaa
     2761 cgatgccgaa gtcgtcgccg agattcagtt aagcgatatt gaactctccg atgatatcag
     2821 ccagggtcag acgacgggaa cggttgtctc catgctttac atcggcgacc actatcagta
     2881 cacggtccgc accgaagagg aagaagattt tgtcgtcgac acccagtatt tatttaacga
     2941 aaacgatgtc gtttccatca acgtcgcccc gcagcacatc ttgcttcgcg tcaaaggaga
     3001 tattgccaaa tatgaacttt aagcgtaaat atctggccat cccgtatagc cttttcttgg
     3061 cgctttttgt catcataccg atccttttga tcgtctacta tgccttctct aatgccgacg
     3121 ggcaaatttc atttgattcg ctcgtccgtt tcttttcttc gacgaccaaa ctgaatgttt
     3181 tgctcgtctc ctttttcatc ggtatcctca ataccctttt gtgtctgatc atcggctatc
     3241 ctctcgccta ttttctggcc cgtaagcaat acaacacaaa taccgtttta gttttgcttt
     3301 ttatcatgcc gatgtggatc aatttcgtgt tgcggacggg cgccactcgc gatttgctca
     3361 cgtggtttgg catcaatggc ggacaatatc cctatttggc gacgatgatc ggcatggtct
     3421 ataacttctt gccgtttacc atcttacctc tttactcgac gatgcttaaa ctcgatcaga
     3481 gccaaattga agcggcagcg gacctcggcg ccaacccttt tcaagtgttt accaaatcaa
     3541 tcctcccgca gtccttgccc ggcatcgtct cggcatcgac aatggtcttc atgccgacga
     3601 tgtcaagcta tgtcatctct gatgttcttt ccgaaggaaa aataacgcta tttggcaatt
     3661 caatctatct taatttctca agttcgcagt ggaacgatgg ctcgttcatg gctttgatca
     3721 tgctggcgat tgtcggcgtc accttgctca tttcccgtcg ctttaataaa gatgattttg
     3781 cctcgcgagg tagcaaccta tggtaaaaag caaaaaactc ttcgcgagca tctatgtcta
     3841 tctcatattg tttttgatgt atcttccgat tctcgtctta atcgcatttt cttttaccga
     3901 ggcgacgaac atcggcgtct ggacaggttt ttcattaaac ttgtacgctc gtctttttca
     3961 aagtcaggag attatggtcg ctcttggcaa cacccttatc atcgccttta tcagcgccgc
     4021 gatttcgacg ctattaggaa ccgctggagc catcggagct ttttacagcc gcaagcgcac
     4081 cctcaaagcc atcgaaggcg tcactcagat tccgattgtc aatgctgaaa ttgtcatggc
     4141 attatcctta accgtcttat tcgtctttgt cggcagcgtc atctttcgcg gacaatcgat
     4201 cttcggtttc tggactctgc tcatcggcca tgtcgtcttg tcggtgccgt ttgtctacat
     4261 ctcggtccgc ccgaaattga tgcagcttga tccgcatcta tacgaggctg ctcttgattt
     4321 gggtgccact ccgcaaaaag ccttgtggaa agtcattatt ccgcagatta tgccgggtat
     4381 cctatcaggc tttctgctgt cattgacatt gtcgcttgat gattttatta tcacggcttt
     4441 taccagaggc acgggtctcc ttaacggaga agggaccatc gaaaccttat cgacgctcgt
     4501 ccaagccaag attaagcgcg ggccgattcc gccggaaatg cgggcgctga cgctcattat
     4561 cttcatcgtt gtcttgctcg tcgtcgtcgg tattactatt tatcgcaaca aaaccaaaaa
     4621 tgtgaaaaaa attcgcaagg ggagggaaaa ccatgaattt taagaagaca ttggccggaa
     4681 catcagctgt tttattctct gcgcttttaa tcgccgcctg tagcggtaat caaccgcatg
     4741 aaggcggtag tcaagttctc cgcatcttga actgggaaga ctacatttat ctcaacgacc
     4801 ccgagaacgg ctatgaggcc aaaaatttag tcgatcaatt tgaagatttt atcatggaca
     4861 ccgaaggaat tgaagtcgat gtcgtttacg acacatttga taccaacgag acgatgctca
     4921 actccttgca gacgggtaaa tcagtatacg atctcgtctg tccgagtgac tacatgattc
     4981 agaagatgat tgctaacgac atgctcgaac cgcttgatga tgcggcggct aacattccca
     5041 attacgaaac gtttgcctcg ccttacttaa agtcaatttt tgataatctc gaggcgacta
     5101 acggcgtcac ccaagaagtt cattcggtct cggaatacgc ggttggatat atgtggggta
     5161 cgtttggtat tttatacaat ccgacatatg cgacttttgc ggaccgcggt ctatcttccg
     5221 acgatgtgat gagtgatatg gaagactgga attccctttg gaatgtcgat tataaggata
     5281 caatttcggt caaagattca atgcgcgaca catactcggt cggcatcatg cgtgtctacg
     5341 acgaagaaat cagaactctg caggatacat atcttgacga agcgaaagcc gaatatgatc
     5401 ccgctttata caacgcacag ctgactgata ttttcaaccg ttcagatgcc gagacggttg
     5461 ccgccgtcaa agaggaactt cttaaactta aagaaaatat ttacggcttt gaaaacgatt
     5521 cgggaaaaca agacattgtc actggtaaaa ttggtattaa tctcgcctgg agcggcgatg
     5581 ccgtctacgc gatggatcag gctgatgaag aaaccgaaac cgagctctat tacaacatgc
     5641 cggaaaccgg tggaaatatc tggtttgacg gctgggtcat gcccaaaagc gattctttaa
     5701 ataaagaatt ggctcagaag tttttaaatt ttgtctcgga tccggtcaat gccgctcaaa
     5761 atatggacta tatcggctac agctcgttta tcggcggtga agatatcaac acccttgttc
     5821 atgagtggta tgacgttcgc gaagatgagt gggagacgcc gattgacgag tggaaactcg
     5881 ccaacgacat caccgatgac tacatcgcct atgatttgac ctatttcttt ggcgacactg
     5941 tcgataccga gacgattatc tatatcgaag aagatagcgt cggccgtcag ctctatgccc
     6001 aatatcccga agcggacatg attccgcgcc tttctttgat gagcgatttc ggcgataata
     6061 atcgctatat tttggcgatg tgggaagaat ttaaagccag ttctttaccg ggctgggcaa
     6121 tcgctctcat cgtcgtcggt gtcctcgccg tagccggggt cggaggcttc tttttatatc
     6181 gcaagaacct cgccaaaaag aatcggagcg cccgccgcca gcaagccgct tcttcacggc
     6241 gctgacattt tttgaagtaa tttattactt attgaaggtt caaaagccat ttgctataat
     6301 tacttgcgct ggttccttag ccaagtggta aggcaattga ctgcaactca atgatcgtcg
     6361 gttcaactcc gataggaacc tccatttatt tcttacttgt ataatgtgaa ctgcctggtt
     6421 tatattatat gagccgccat cttggcggac acgcgcccgt agctcaactg gatagagcgt
     6481 tagactacgg atctaaaggt tacgggttcg actcctgtcg ggcgcgccag tgtcgggatg
     6541 tagcgtagct tggtatcgcg tctgctttgg gagcagaagg ccgcaggttc aaatcctgtc
     6601 atcccgacca ttactatcca agctctgatt aggagtttta ggggatatag ttaaacggga
     6661 taacactagc tttgcaagct tgaattcgga gttcgattct ccgtatctcc accaaacgaa
     6721 atggctgcgt agctcagttg gctagagcaa tcggttcata cccggtgggt cgagggttca
     6781 aatccccccg cagctaccat tttcggacca atagctcaac ggttagagct ccccgctcat
     6841 aacgggtagg ttctaggttc gaatcctagt tggtccacca gacgtggagg cttacccaag
     6901 cggctgaagg ggtcggtctt gaaaaccgat agaggatgca agtcctgcta gggttcaaat
     6961 ccctaagcct ccgccattca cgatacttaa gtcatatccg cgggtatgat tttttattta
     7021 tttaagccga tttttatata ataagatgta taacatattg acaaccatca atgtgttaga
     7081 atagtgttta tgtatggcaa aaataaatga tgagcatttt attcaactca tgaaaatact
     7141 tagcgataca acgcgttttg cgatcgttcg cctgcttgct caaaaaggca gtctttgcgc
     7201 ttgtaagatt ctcgattgct tgcatatatc gcaaggcact ttatcgcatc atatgaaggt
     7261 tttaacaaac gcgaaaattg tcagctgcag caaagatggt aaatggcggc attatcgcct
     7321 tcttccacat tccttataca aattatcgac ttttattcaa gagatcactc ttattccttc
     7381 cgagacgacc gaagaaaact gtgcatgctc aattgatgtg ataggcaaat gaccaaacga
     7441 aaaataacta ttattatcgc ttcaagtgtg gtggcactat tagcggccgc taacattttg
     7501 gttggcatta ttgtttcttg ggatgccgca aaagcgccat ggaattttat tagttcgcag
     7561 attttgggca tgaagtggct caacgatttg atatggaaaa ttgtgcaagc cgtcagtggc
     7621 gtacacagtt tagagacatg gcaatacaaa gttcaatcgt ctatccagtt ttttgtctat
     7681 gacattatca aaattaccgt actattatgc tttttaatct ttgtaatatc gtacattcaa
     7741 tcgtattttc cgcccgaacg cacaaagaaa atattgagca agtatcacgg aatcggcgcc
     7801 aatattatcg gggcattact cggaaccgtc actccttttt gcagttgttc ttctatcccg
     7861 attttcattg gatttacacg cgccggtcta tcgactggag taacgtttag ctttctcatt
     7921 tcaagtccgt tggtggatct tggctcctta atgcttctgg tcggggtttt tggctggcca
     7981 atcgcaattg cttacgtcgt cgctggcctc attatttctg tcattggcgg aacgattatt
     8041 gaaaaaaccg gactgggaaa acatgtcgaa gactttgttt caaagggcga gagcgtcgag
     8101 atagaaagtc cggaattaac aaggaaagat cgcataattt acgctcgcga ccaaatgtgg
     8161 ggaacattta aaaaagtatt tccttatatg ctgattggcg tggcgatcgg agcggcaatt
     8221 cataatgtta ttccgagaga atggattgaa aacattttag gatccgaaaa atggtatgga
     8281 gtgcctttgg cggcccttct tggcgcaccg atgtacgccg atattttcgg gacattgccg
     8341 gtcgccgaag ccctttacaa caagggcgct ggcctcggca cagtcttgac ttttatgatg
     8401 agtgtgacag ctttatctct tccgtcatta atcatgctgc ggaaagccgt taaacccaag
     8461 ttgcttatcg cttttttaat cattgttctc atcggaatta ttgctatcgg ctatctattt
     8521 aatttgtttg ggtacttttt catataggag gaaaaatatg aaaattcaag ctttaggcgg
     8581 atgttgcaaa aaatcaactt caaattatga agcaatcgtt caagccgtta aagagttaga
     8641 cctcaaagtt gaggtcgaac acgtcactga ttttggcgaa atcatgaagc taggtgtcat
     8701 gtcaacaccc ggccttgcca ttaatggcaa aatactttca gttggcagag cactcaatgt
     8761 catgcaggca aaagaattaa ttaacaaggt tatgaagagc gaaagcgaat gctcctgcgg
     8821 cgaacgatgt gattgtggcg ataattgcga atgcgaagat ggctgtgatt gcgccgatga
     8881 cccttcttgc tgcaaataat tatcaataaa tattattgct ctctaatact ttgctaaaat
     8941 agaaatgcaa atcattggag gttttctatg cagcatttat tagaccaggg tccgctttta
     9001 aacgaggaag gcaacttaac agaatctggc tatgcttttt cgttaatcaa aaaatattct
     9061 cgcgatgcca ttaaagcgcc tgctatgcgc atcaaagaat gggattacta ctatgtcgga
     9121 aacaatcatg tcggcgtagc tctgacggtg gccgataacg cctatatgtc gctcgtcgga
     9181 gcgacggttt ttaacttcga caaagcctat gacaaaacca agaacgtgat gggcttgctt
     9241 cccaaaggga aaatcatgat gccctcttct tcagcaagtg gcgatgtcat ctttaacacc
     9301 aagaattttt ccttccattt cactcatgaa aacggcaggc gtcacctcgt ctgcgagatg
     9361 aagaacttcg atggtgaaaa gccctttcgc tgcgacatat atctcgagga aacaatcaaa
     9421 gattcaatgg tgattgccac gccttttaat aaaccgaaac acttctatta caatcagaag
     9481 atcaatttac tcaaagccaa cggttacgcc aaagtgggcg atgagacata cgatttatca
     9541 aaagacacat ggggcgttct cgactggggt cgcggagtgt ggacatatag taacacttgg
     9601 tactggtctt cgatgagcgg tacctacaag ggcaagaaga tcggattcaa tctcggctat
     9661 ggttttggcg acacaagtgc cgccagcgaa aatatgttct tttacgatga taaggcttat
     9721 aagtttgaag atgttaaatt tgatattcca atcgccagca atggaaatga tgatttcctt
     9781 aaaccatgga agtttcgcag caaaacgggc gatatttctc tgacatttga accgatatta
     9841 gaccgtttca gcaacacgaa tgttctcatc atcaagtcga ttcagcatca ggtattcggt
     9901 aaattccacg gctattttgt catcgaaggc aagcaggtat attttgaaga catcctcggg
     9961 tttgccgaaa aagtcaggaa ttgctggtaa ttttgcgatt ttttcgtgtc tctttttaga
    10021 taataagttt acaataaaca ttagttaata aggagcagac tcatgaaaat taactccact
    10081 tttaccgggg aaatcgtccc gcgtgcgacc aaatggattt attcggcgac ggcgatggga
    10141 agagatgcag cctatacttt aatcagctta ttcttaattg cctatattca attcgcggct
    10201 ccgattggct atgttaatga cgtttatagt cgaggcgatt acttcgccca gtttggcgtc
    10261 atttccatca tcgtcatcat cgcccgcatc tgggatggcc tcaatgatcc gatcatgggt
    10321 tttatcatcg agcgctgcca ctttaagatg ggtaaatata aaccttggat tctaattggc
    10381 gcgatcacta ataccgccgt catgctcgtc ttgtttttag cccgcccgca aggctgggct
    10441 tatgtcgccc ttttcggagt cttctacttc ctttgggaca tcacatacac gatgaacgac
    10501 atcggctatt ggtcgatgct tccggctttg acaagcgacg aaaaacagcg caacgaaatc
    10561 acgacgatta tgtcgatatt tataagcatc ggagccttcg ctgtcggcgg tgttgtgcct
    10621 ttgacggtcg ccaataatgc cgtccaaaac tatggcatca tcgctatcgt cgtctcagtc
    10681 gtctttcttc tctcgcagtt aattcttgtt ttctttgcaa aagaatcggc ccgtaatctc
    10741 gagcaggaaa aaatttcgca aaagacaaaa ttcggggata tgttccgcct gatgaagacc
    10801 aacgatcaac tacgttggat tgtcatcgtc atcctcatct actatatcgc ctcgaacatt
    10861 cttaatggcc ttggtctcaa ctatttctac ttcatgtttg gttatggaaa tgacgccggc
    10921 gaaggcggaa atattcagtt cctgtttaca atcatgtatg ccggaggaac gattctggct
    10981 caattcttgt tcccactttt aataaagttt ctaaagcgca gcacactgct cttgatcagc
    11041 tttttgatga tggctttagg ccatgttgtt ttcttcttca tcggcgcgcc gtttaacggt
    11101 gttccggccc tcggcgttca atatccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnccgg ggaaagaaaa gaagcggtgg cattctcgct caggccgctg accgccaagc
    11281 tggccagctc tgctcagcaa ggcatcgtct gggtcaccct ttctgtcagc ggcgccctcg
    11341 ccgtcaccga aggcatctca aaacttgagg tcgataaggg tcttggcatt gctactgaca
    11401 tcgaaaccgg cgccaatcag ctcatcgcta acggcaatca ggatatgatt atctttgcgg
    11461 ctgtcatgac tcttcttccg ttccttcttt atgtcggcgc ctatctcatc acccgtttcc
    11521 tatacaatat cgatgaaaag aaatacgccc agatcgtcaa agaagtggac gaaagacgcg
    11581 catctcttgc tgttgaaaca ccgcaagaag aagttaagta attataatat aattaaaata
    11641 gcgacctgat tggccgctat tttttatttg acttcgaatt cgactttcgc cccatattct
    11701 tcgccggaga tttccagggc ggccttgcct ctttgtttga ggcttcggac atagaccgaa
    11761 attcttccgc cgttaagcgt gataatgctc ggcccgatga tttgaatcgg tcctctagtt
    11821 tttataaata atggcagggt tgagtaatta cagagcgttt catattgatc aaacaaaccg
    11881 atttcgacgc gggcgacatc gtaagtatcc gcgtttagta aaacattttt gttcgccctg
    11941 gccttgaggc tgaactcggt cgactggcca aacatcttgc tggccacgag ttttccgtcc
    12001 ttataggctt taaatttgaa gaccatcaat ttcgcgcccc acatgccgac atacttattc
    12061 cagaggtcga cgagctcatc atacgacatc ctatgtttaa acatcactaa tcccacgcgt
    12121 attttgtgtt taaacggcaa tttattaaga ccgtggagag cgacataagc cagggcgtcc
    12181 ttgatgcgtt cggcatcgcg actattaaac ttggcttcgc tgaaagaggc gccgatataa
    12241 tcatcgactt caatcggagg atggggcatg tggcgatatt tggcgcggtt gggatagaag
    12301 gctttgacga agacatcgtt gcggtagagt tcgatgtaat cggcattggt ggcgacgtgg
    12361 gtgacgccga cgatcgcctc gctaaaatct ccgggtgtca gattggacag cacttcaaag
    12421 acgaaattat catcgctttg cgatgcatag atagaagccg catactttgg attgcggaac
    12481 atgtcgagga cgccgtggtg gcagacatgg tcgccggagc caaaatcgcg atgcgtataa
    12541 taatcggcaa aacaccagcc gatggcaccg atgagattgc cataggcgaa attatcgtcg
    12601 atgactttag cgtgtcgcaa ggcatggtga atgcgccgct ccattgagtc cagagttttg
    12661 gtcggataca tgtggccgag gtattcggtg acgagaaccg gagctttgcc ggcctcaacc
    12721 gtttttggat tatcgagtcc gtgtgacaaa tcttccgaag aaaaatcatt gaaggcataa
    12781 atatcttcga gtaattcgct gtttttaaag ttgcggacgc cgagggtcgg gcggccggga
    12841 tcgagttcgc gggcaaggct gtttgaagcg gcatagagtt catgatcatc ctggctctca
    12901 ttgatgcgga cgccattggc gatgaggctc ggatgattgt gttcttttag aatcatcctt
    12961 tttacgaaat cgagaaagtg gttgcgccaa gtctcgtctt ttccgacata ttgccagccg
    13021 ggaatctctc cgatgaccaa taagccgatt tcatcgcagc ggtttaaaaa gtgttccgac
    13081 tgcggatagt gagaggtacg gacgatattg acgccgaggc gatactttaa gatatcggca
    13141 tcgtcttcct gagcgccttt aggcatcgca tagccgacat atgggtaaga ttgatggcgg
    13201 ttgaggccga tgagttttat ccttttgtcg ttaaggtaga aaccagattt ttcaaagcga
    13261 atggagcgaa aaccgaatcg cgtgacgacg aggtcatcgc cactcgtcgt tttaagcgtc
    13321 gtcaaaagcg tgtataaatg cggtgtatcc aagttccaga gatggggatt ttcgatttgc
    13381 agttcgttct cgctaaattc tttgatgact tcatccttaa aaagaagttt gtgcgaaatc
    13441 tcgtagttta aggaatggtt tccgccgaga tgagggttga tttttaacat tccctgccga
    13501 tcggcggtaa ctaaaaccct ttcaagatag atattcggtt taatttcgag ttcgacttcg
    13561 cggtagatgc cgccaaaggt cagatagtcg atgacaccgc caaaaggggg aatactatta
    13621 tcttcatgag agtcgacgat gacgacgagg cgattatcct tggctttggc gatgccggaa
    13681 acatcaattg taaaggggag ataaccagag acatattcac ccaaaaaggc gtgattcaga
    13741 tatatttttg ctttgagcat cacgccgtga aaagtaagaa gaaccgtctt gttttttagg
    13801 cttgtttcga tatcgaacac tttctcgtaa gaagagataa attgatagat atcttcgtca
    13861 aaataggcgc gcggcaattc aacattggag tgaggtaaat caatcttttc ggcttcttca
    13921 gggaaggcat ttaaaaaact ttgtttataa cacggcatat aacgccaagc gaagttcaaa
    13981 ggctgtttca tgattccttc ctttccgtag gtgcaaggtc gctcacttat tatataatat
    14041 tatgcgagga acgattatga taacctgcaa agaaacactc tttcatattg agacgagcaa
    14101 gaccagctat gtcttgcgcg tcaacgagat taatcacata gtaaatgaat attacggagc
    14161 aaggctaaaa aatctggatg attcgagcgc gatcatcgaa aaatggccat atgcctatgg
    14221 cagttctgtc atttatgacg aagataaaaa caaagctctc tccctcgatc ttttatcgct
    14281 agaaacaagt tgtgtcggca agggcgatta ccgggaaccc tcggttaaaa ttagaagcgc
    14341 ggatggatat gtttttgatt ttacttataa gcatcatgaa gtgcgcaaag acatccagcc
    14401 gctagaaaat cttccctcac cgcgggaggc tgatgaagaa ttgattatct ttctcagcga
    14461 tgattatgcc aaagtcgcct tagaactcca ttatcttgtc tttgcggaca gcgatgtcat
    14521 tgcccgcaat gttgtattaa ttaataatag cgaatccccc ctatacttag aaagactcat
    14581 gtcgatgcag ctcgatctaa tcaatcgcga tttttcgctc ctcaacctct acggaggctg
    14641 gatcagcgaa gcgcaaaaaa cgattcgcaa agtgacaccc ggcatctatg ttaacgattc
    14701 gaagacgggg gcgagttcaa accgtcataa tcctttcttc atgctcatgg aagacaaagc
    14761 aaatctcaat agcggtggtg tctatgcctt taatttgatg tatagtggca atcaccaaga
    14821 gctcgtcgaa ctctctaatt atggtaaagt gcgcgtccag ataggcatta atccttattg
    14881 ctttgacttt aaagtcaagc aaggcgaggc tttcgttaca ccctatgctg tgatgaccta
    14941 tggcgatagg ggtttgaatt ccgcctcaca aaatatgcat tcattcgtta atcatcatgt
    15001 tattaagaaa gcttttgaaa tgatggaacg cccgattctt ctgaataact gggaagccac
    15061 ttattttaaa ttcactgaga ccaaactctt aagcctggct cgcgcggcga agaaaatcgg
    15121 catcgagctc tttgttcttg atgacggctg gtttaaaaac cgcgatgacg acaccaaggc
    15181 tttgggcgat tatgaattaa ataagaaaaa gttgccgcgc ggtttaggca cgctcgctaa
    15241 aaaaatcaat gatttaggtc ttaagttcgg cttgtggttc gagccggaga tggtcagcga
    15301 agattccgat ttattccgcg cgcatcccga ctggattatc ggcaatcgcg aacgcggcat
    15361 ttctaaagga cgccatcaac tcgtattaga tctttcgaga aaagaggtgt gcgattacat
    15421 tgtcgattcc ctcgatgagg ttttaggttc ggctaatatc gaatatgtca aatgggacta
    15481 taaccgtcac atttccgact tcttttcgtc aaagacgaag gctggagagt tgctccatcg
    15541 ccaggtcctc ggcctatatg atgttttaaa aaggattacc gacaagtatc cgcatgtctt
    15601 gtttgaaggc tgctcaagcg ggggcaatcg gttcgattta ggaattctcg cttacatgcc
    15661 tcagacgtgg gccagcgatg acaccgatgc ctatgagcgc attcacattc aaagcgggta
    15721 tagccttgct tatcccttgt cgagcatcgg agcccacgtc agtgcctcac catcccatca
    15781 gctgttaaga aatacgccga ttgacacgcg tttcaatgtc gcgtctttcg gcgtcctcgg
    15841 ttatgagctc gatttaagtc atcttgacga ggtcgagacg agggctgtca aagcgcagat
    15901 ttctttctat aaaaaacatc gccgtcttct ccaatatggc gctttctatc agatgcagtt
    15961 aatcgatgac gagaatgcgg cggtgtggat ggttcttagc gaagacaaaa aagaaggtat
    16021 catcggctat tttaatggcc tgcaaaaacc gaatccgaaa acgacgatat tgcagggcat
    16081 cgacttcctt gaagatgaaa gatatgcgat gaatgtccgt cctcaagacc ataacatcaa
    16141 gaagttcgga ggcttaatta atatggtcct tcctattcgc gtcaacgaaa acggttttct
    16201 agtgaatcaa atcgctcatc acaaagtgat gccgggcgaa aaagaaagtt atgtcgttac
    16261 cggggccatg attaatcacg gagcgattaa gctcaatccg gaatgggccg gtgtcggcta
    16321 cgacgaaaga gttcgcgtcc tcggcgattt cggctcccgg ctttatcatt ttgaaattat
    16381 tgggaaataa taagaagcgt atttgcgagt ccgagcatga tcaatagctg gctcgcataa
    16441 aataagagca tgatgtaaaa atcgcggcgg cgcatatctt ttccgtatgc ggaatagacg
    16501 agtaaaaaat ccgatagaaa gtaaagggca tagccgacta aaatcatcgt tgcgataatc
    16561 ggtttgccat caactaaaag aagaatcgct aaagcgatgt taataagatg caatgacaaa
    16621 tataagttga taatgtaggc gatttttcca aagagaaggc gcgataacgg ataaagtgct
    16681 cccgagaagg caagcgatgc gccggcgata gcgacataaa tccaccatgg catcgtgtag
    16741 cttagcatcg agaccataac atatgcattg agcatgtgtc cgacggtgta aaagatggag
    16801 ccggtgataa aaagtggtcg cgatttgatg cggagagcga ataaatcgcc aaagacgcac
    16861 atgattcccg cgagccagat ggcatacttg ggaaatggca tttccggata tgtgagagtc
    16921 aaaattagaa ttgcgccggt aagcagtaaa gtcgtcagcg gtttggtcgc ctttcgccac
    16981 gcctctttct cgagcaaaca aaaaattaag tgaatgaagg aggctaagcc gaagacgccg
    17041 atgaggacgt aaaacgcgat ttccataatg tgcatatcat tatacctaag tggtcaaaaa
    17101 agtaaaatat ataaaatata tcgcttgcaa attgcttttg cacttgttaa tataacatgg
    17161 ctaaagacac gtcgcggggt agagcagtct ggcagctcgt cgggctcata acccggaggt
    17221 cggaggttca aatccttccc ccgcaaccaa ttacaaacaa aagagccagc tggctctttt
    17281 tttgtggttg ggggacagga ttcctccgac catccgggct aaccgtcgta gcgtttcgat
    17341 atgtagtcta aatcggcata gtagttgggc caataaaact cgagtcgctg ccgggatttt
    17401 tcgcgtttaa tttcttttcc gacataacgg gcatgaggcc tgagaagaat cggtccgata
    17461 tgcaaactgt cgataaatgc ccagtcgcgg cgatgacaat gttttatcat ctgttttttt
    17521 gaaacgagaa tcccgtaatc cttatcgccg tgataaacat agtcagcttc aatgttttcc
    17581 tcccgcgctc ctttaaagac gacgcggtta ataatgctta tgacgaattc tttgtcgcga
    17641 tttaactcaa gatttgcctc ttttaagcga tccgccagca aaaagcgcag tttttcatag
    17701 ggaaaccgct ctttgccggt gccatcaagc gtgccgtctc caaattgata aagcaaaatg
    17761 gtctgcagcg tccgattcga aatgcctctt tctcgcaaat atatacaaaa ctctttgaca
    17821 tattcttgat ggagcgtatt gctgctgccg cttttcatcg agatataatg agtattttta
    17881 cctatcctga tgtaaaaatc tggtttgatg aactcatcca gccgtttggc atagacaaca
    17941 gcctcttcgt cgggatgctc gaaaatctcg cggattatat acttcgcgtt gttagaaagc
    18001 tgacacactt tcttttcgtt gagcatcagg acaatttcct cctctttgta aaaacctaca
    18061 ttcttgtgca taaattcctc ctactgatac atatgggtaa acatcgggaa aatattcaaa
    18121 aaataatttc tatttttctt cctttaccac tctgataaga cttgtaggtt gttttctacc
    18181 tacttttatg ataaagtct
//
LOCUS       DAOSSL010000019        17005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_3481_length_17005_cov_16.659469, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000019 DAOSSL010000000
VERSION     DAOSSL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_17005_cov_16.659469"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLH17_02920"
     CDS             complement(<1..1520)
                     /locus_tag="PLH17_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOR20757.1"
                     /translation="MSNKPYKGFSPKWVETPADPRSWRSIFRWGDPHYFKWPKENLYK
                     VMKEIFDLTDDDFQKYDGGLGFGPVDYNVPSCLAPEHIDAFKALLGEEFVRTDSYSRL
                     SVAYGKTMHDVLRLRQKIVENIPDAVLYPDNREQIEKVIAYCSTHKIPVYVYGGGSSV
                     TRGVECVKGGVSLDMRLRFNKVIAFNEKDQTITVQAGMSGPQLEKTLNDAEKLFGAKR
                     AYTCGHFPQSFEYSSVGGWVVTRGAGQNSTYYGKIEDMVLEQKYATPIGNIVTSPYPR
                     EATGPSLNHIMMGSEGAFGVLTDVTLRVFRLTKENRKCFSFMFHNWEEAQEATREMMQ
                     CEAGFASVFRCSDAEETDLMMRLYGVDESPLAKLFKIGGYKPMERSLFLGWSEGEKGF
                     AKNVARNIRRIAHKHGGMSLTGIVTKAWEKGRFTDPYLRDSMQDFGILTDTLECSVLW
                     SNMAEVHKSVRAYCHSRPHTVVTTHLSHAYPQGANLYFIFITKMNDINDYIAYHSG"
     gene            complement(1520..3079)
                     /locus_tag="PLH17_02925"
     CDS             complement(1520..3079)
                     /locus_tag="PLH17_02925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOR20758.1"
                     /translation="MIMNPLVLTFDCGTQSIRSLLVDKQGNIVAKEQISFIPYYSLQP
                     GYAEQKPSVYIDTLKEVSRRIHQNNPDKVAQIIGVTITTIRDTCVCVDKDIKPLRDII
                     IWLDQREAKCENPLPLMARMAFKLVGMSEALEDQRRITKSNWIQENEPEVWAATYKYL
                     TLSGFLTYQLTRKLIDSSASQVAHIPYDYKTNKWYEPSNIKFAIFNVDTDKLQDILPP
                     GSTLGTISKEVSEEYLIAEGLPVIATASDKACETLGCGVLDNDLASLSFGTTATIQTT
                     LRKYVEPQQFMPAYPGATIGTWNPEIQVYRGFWMISWFKKEFAEKEVLQAPKLGLSPE
                     ELLNRRLKEIPPGSDGLILQPYWAPTLKQPEAKGCIIGFSSEHTRVHIYKAIIEGIGY
                     GLLEGMQNLEKSSKQKISGLTVSGGGSQSDEICQITADMFGKPVYRIQTYEACGLGSS
                     MVAFVSLGVFKDYREAIQSMVHYKKTFTPNPEVHEVYRNLHSRIYKRIYKQLKPLYWE
                     LRSITKERKDK"
     gene            complement(3076..3441)
                     /locus_tag="PLH17_02930"
     CDS             complement(3076..3441)
                     /locus_tag="PLH17_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOR20759.1"
                     /translation="MKKSLTCIVCPNGCRLEIDVQSHEVIGNKCPRGIAFAINELTDP
                     KRSITTTVRTTLPEYPVISVRTDGDIAKDKIMDLIHLLKDVVVTEYLPIKTIIVHNVF
                     GTDVNVITTANMQKGDKCL"
     gene            complement(3438..4685)
                     /locus_tag="PLH17_02935"
     CDS             complement(3438..4685)
                     /locus_tag="PLH17_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR20760.1"
                     /translation="MKRYDVIVIGAGPAGLAAALKADENGASVLLIEREAKLGGILKQ
                     CIHDGFGLTRFNEKLSGPEYANREIEKIKKTGVSVNTLTFVTKIEKNDDSFTLTLVTR
                     DGIASVIGTKLILATGCRERTSRQIFIGGTNPSGLMTAGAAQYYINILGKMPTKRCVI
                     LGSGDIGLIMARRITLEGGQVLGVYEVKPTPSGLTRNIVQCLDDFNIPLHLSHTVTKV
                     FGCDRLEAVEIAEVDEHMQAIEESKHIVACDALIISVGLIPENEIAEQLKIAINPKTK
                     GPYVDQTLMTFAPHVYSCGNSLHVSDLADYVSESGELAGTYAAKSIQKERDLQEIEID
                     SHLMYFVPSHLDFKSPTRQVIAYFRSAGEYENATLYIEHDGKTLFKKRYLHLRPPEME
                     RIVFSLSPDLAPATVRVYLEVKP"
     gene            complement(4682..6394)
                     /locus_tag="PLH17_02940"
     CDS             complement(4682..6394)
                     /locus_tag="PLH17_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR20761.1"
                     /translation="MMKPTFEKTIAEISRLYPGVRTTIKDGCLLLEGEMEKWDEVVKV
                     GYIAAQAKSEGVINNIKLKGYTPEPMRISSIQDSEYDGLKCDALIVGGGIVGTAILRE
                     FSKYDVDACLIEKENDVALHASSRNDGCIHVGMDLSRKTKKHHYLRRAVQSYEQLAED
                     LGVDYVKHGQTLVFTKTLAYLAMPFLFSAARKKGIKGVRLLKREELLKREPNIGKEAK
                     FAVFFPEGAVVSPYNMVVALAENAIENGGRVLLDTAALKFEVKDHRIISVKTNRGIIY
                     PRVVINAAGVFADIIAGMAGDQFFSIHPRRGVEMILDKKAASKSVNSTLSFYAGNSDK
                     KAHTKGGGIIPTVDGNTVVGPTASECPEREDYQTTKEEIDALFDKHRHTLPSLSRSDV
                     ITYFAGIRASTYEEDFIVRRGKWTENIIHAAGIQSPGLTAAPAIAEDIVKIYEMVSGE
                     KLAPKKQFNPTRKSNRLLKELSVEERDKLIKSNPDYGQIICRCEEISKGEILDCLRRP
                     LKVHTTDGVKRRIRAGMGRCQGGFCQQLIVQIMAEEQKALLTEIKKKGEAPILLDDNK
                     GGMR"
     gene            complement(6391..6960)
                     /locus_tag="PLH17_02945"
     CDS             complement(6391..6960)
                     /locus_tag="PLH17_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR20762.1"
                     /translation="MARPKGSKSKERIIAAVEDLIATKGVKNFSLRDVALKASLSPGT
                     IYYHYTTKDELVFDVISQHIDALKTEYILWLDRHENDLPPERFLEVVFHKSVKLFDRA
                     KMHIFLINECLGENDVLRSKFIEKYREWRATLLIGIEKVFRQVDDPEAFAYTLMLMID
                     GLVVQEILKDDSFDEKRLVQFAKSWGGNR"
     gene            complement(7097..8134)
                     /locus_tag="PLH17_02950"
     CDS             complement(7097..8134)
                     /locus_tag="PLH17_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20763.1"
                     /translation="MNKFNKLLFSALSLFALTVMTSCDGNGITIEAAVANQRVMDAME
                     QMTTTEIESFEIDITADLDYSGKAFDADDVVLFERNLEAAGEVHIKANDIFGEDAVGS
                     VTASASVLAVENDETLVDVDAGVAVYLSEGWVYADVTDATELFALMDQEAPEVTTIKT
                     YVGNLGEAIGYNPEEPIEMPFAFDDMVPYANLLSDVKATESNGELTVVYTVTIDDIVN
                     IGVKVMQDAGEIPADATSEEIETFRTQMREMISSVINITTAKLTIGVGADGFLSKLYV
                     DVDVAVTIAQEEETGVYMRAEEPVIDHELHELEGFFHIDIDNMNQVVDVVLPDNLDTY
                     TEIPQEEEPTV"
     gene            complement(8253..9617)
                     /locus_tag="PLH17_02955"
     CDS             complement(8253..9617)
                     /locus_tag="PLH17_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOR20764.1"
                     /translation="MIEEIVQKQRAYYFEGHTRSLDARREVLRKLRKILLDMHQDIYD
                     AFKLDYNKCEFDVISTEYSLVIQEIDFMLHHLKKFMKPRRVATSLVNIVSSGYIYKEP
                     YGVTLVMSPWNYPLQLAMVPVVAALAAGNTVVLKPSKYAWNVSLVLQKIASNFDPRLF
                     SVVVGGREENQALLNQKFDFIFFTGGDDVGRVVLEKASVHLTPVILELGGKSPCIVME
                     DADIDMAAKRITWGKYLNAGQTCVAPDHIYVHESVKDTFISRVIEYIQAFYYHDGNIS
                     EDFPSIINDKHIERLAGLIDKNKIVFGGHINKDKRLIEPTIMDNVTYDDAIMGEEIFG
                     PIMPILTFTKVDDLLEIFKSRDKPLAFYLFTKNQKEARRIMDLVSYGGGCINDTIMHL
                     TNDKLPFGGIGRSGMGSYHGQKSFEAFSHEKSVLKKQKMELQLKYPPYNEKKKSFIKW
                     FFKL"
     gene            9727..10209
                     /locus_tag="PLH17_02960"
     CDS             9727..10209
                     /locus_tag="PLH17_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20765.1"
                     /translation="MNIFYLDPDPVIAARYHADKHVIKMILESAQFLSTTHHIAQSSQ
                     ETLDNIYKPTHFNHPSTIWVRQSKANYLWLFSLFLALIDEYAFRFGKTHKSAELIPYL
                     EKAPKALSDSLFSPPTPAMDEEYVVDGDSLLSYRNYYRLAKSHLFAWTKRERPEWLKD
                     "
     gene            10287..11528
                     /gene="glyA"
                     /locus_tag="PLH17_02965"
     CDS             10287..11528
                     /gene="glyA"
                     /locus_tag="PLH17_02965"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOR20766.1"
                     /translation="MNELKTIDRDIYDAIELEKKRQRENIELIASENFVSLAVLEAQG
                     SIMTNKYAEGYPNRRYYGGCEFVDIAEQLAIERVKKLFGVNFANVQPHSGSQANMAVF
                     RALLNHGDRILSMSLSDGGHLTHGYKLSFSGADYEIYSYGVDAVTEMINYDEIERLAL
                     EVKPKLILAGASAYPRAIDFKRFREIADKVGAFLFVDMAHIAGLVAGGVHQNPCPYAH
                     VVSSTTHKTLRGPRGGIILSNDAEIATKIDRAIFPGIQGGPLMHIIAAKAVAFKEALE
                     PSFKDYVKNIVDNAHALADELKKLGYRIVSGGSDNHMLLVDLKASIGMTGAEAEKLLH
                     EVGITCNKNSIPNDKERPMVTSGIRLGTPAMTTRGFTSRDFRLVATLIDAALKNRDNA
                     DKLAEIRQKVLELTREHPLPY"
     gene            11528..12043
                     /locus_tag="PLH17_02970"
     CDS             11528..12043
                     /locus_tag="PLH17_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOR20767.1"
                     /translation="MMINTESKLIYQMIAIAMGVALSVLLTSFVYIPLPFGGLFNFTD
                     VFIVLYSMFFGPWIGGAVGALSGLLVDIILGYNFYAPFTFFIKLIEGLIVGYLFLKMP
                     KKIRYVGPFIGGIVMAALYIIPDFILMQNWIVALTSFGINLLQGFLGASLGIGIFLVL
                     IKAKVRPPTRS"
     gene            12124..13548
                     /locus_tag="PLH17_02975"
     CDS             12124..13548
                     /locus_tag="PLH17_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOR20768.1"
                     /translation="METIQDSKFKRASRRGVLAFQHVFAMFGATIVVPLLAGLSVPIG
                     LLSAGIGTIIFYFLTKKKVPVFLGSSFSYLPGIIAYMVSQNAGTVGTESWQRAVGGLA
                     PAIAITGLVYVILSIIVKFVGMQRIKKLFPPVVVGPIIIVIGMVLAPKILYNNVIGQY
                     TNGAMDAWKAWTIAIVTLLSIVAVSTASKEKSFFRVTPILIGFAIGIITTITIDMIEL
                     YALSGDIAKTIIFYKFSSAGALANGIDWSQIVIFQNIGQTFGFYKYLRLDLNAVLLIS
                     PIAFVAFMEHIGDINASSAVCGKDFTVDPGINRTVLGDGLATVSAAFLGAPPKTTYGE
                     NTAVLAITQNYDPFNLFLAAIFATVLGFFTIFGNLTLTIPAPVIGGASIVLFGMISAS
                     GLKTLISSRINMNNTKNMIVVSVIMSLGLGLAALSLVGDITGNNIYKIVIGSVEISPL
                     AVSALAGVILNLVLPDRQKEIATI"
     gene            13649..14212
                     /locus_tag="PLH17_02980"
     CDS             13649..14212
                     /locus_tag="PLH17_02980"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOR20769.1"
                     /translation="MWDALENIGTWGILFAVLIIFLAKIVEVTIGTLRQILVVKGYRT
                     LAVLLALVEIFLWVFIASKVINDLSVNIFYGISYGLGFAAGVYFGSIIEQKLAFGMLL
                     VQTITTEKKGVEIANALREKGYGVTTVDAKGKVDSRKILMVYANRRGSQEIVEQVMLI
                     DPTAMIVTNDVGTLSGGYIRIKKSLLK"
     gene            complement(14236..15339)
                     /locus_tag="PLH17_02985"
     CDS             complement(14236..15339)
                     /locus_tag="PLH17_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOR20770.1"
                     /translation="MTKQEKQELNNEKPDAEPGKKWFSRFMDHLTKTTGGMAIGMFAT
                     LIIGVIIGQIGTLSNIQFIVGIGNILKGLMGIGIGIGIALSLKNLSPIAIISAGVAGA
                     VSTMVLGIDNGAYVLPFINGTSTNGNPLMAYLVVVISIELYRLVFRKQTAVDLIIVPL
                     FIAAVAAALSFLFAVPLTWIVTILGQFIQTATAYQPLLMGVVISSVMGMILTAPISSV
                     AIAVAINLGGIAGGAAAVGCCTQMVGFMMMSIRDNNVGKVISVGIGTSMLQFKNILRK
                     PLIWLPTIIVSAVLGPLATTVFMLHTTPVGAGMGTCALIGPLSTILEMGDNPLMAWLG
                     VLLLMIIAPAGLVFLIDLLFRKLGWIKKGDLTI"
     gene            15457..15984
                     /locus_tag="PLH17_02990"
     CDS             15457..15984
                     /locus_tag="PLH17_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOR20771.1"
                     /translation="MNQKKSLFREIIRFIIVGVIATVLDYGTYSLLALAIPDSWSPIL
                     ETVICTTIGFIVSVIANYLLSVIWVFQNVDQKADVKSKKNMFLFVVLSAGGLLLGTAV
                     MVGFEALASNALHVDINNWISDFQINYLRSSAFWLFTLFFGIKTLVILSYNYITRKKL
                     IFKSPKENEDGQITD"
     gene            15959..>17005
                     /locus_tag="PLH17_02995"
     CDS             15959..>17005
                     /locus_tag="PLH17_02995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOR20772.1"
                     /translation="MKMGKLRINMLSKADSVDGQGVGSAYLEQVALVKECDDIFNVTV
                     NEGGSKFDIVHVHTINPQYYLKMNNKHVNVCYVHMLPDTLDGSIKLPKGLFKLFKRYV
                     TKFYRKADEIVVVNPIFINPLVDLGIKKDRITYIPNYVSKDQFHVISEEKRMEVRDLY
                     GVPRDRFVVLGVGQVQTRKGVLDFVETARRNPEMFFVWAGGFSFGKITDGYKELKEIV
                     DNPPANVKFTGIVKRDKMNDIFNMSDVLFMPSFNELFPMAILEAVNAHKPVLLRDLDL
                     YVDILFEKYEKGHGVDDFVQVLRNLKADAKIYQKASENSAYISEFYSKEHVREIWREY
                     YPRVYKKYAGLKKFP"
BASE COUNT         4262 a         3996 c         3995 g         4752 t
ORIGIN      
        1 atgccgctgt gatatgcgat atagtcatta atatcattca ttttcgtgat aaagatgaaa
       61 tagaggttag ctccttgcgg gtaggcatgc gaaaggtggg tggtcaccac agtgtgtgga
      121 cgcgagtggc aatatgcgcg gacgctttta tggacttcgg ccatattcga ccaaagcaca
      181 gagcactcta aagtgtcggt cagtattccg aaatcctgca ttgagtcgcg aagatatgga
      241 tcggtaaaac gacccttctc ccaagctttg gtaacgattc cggtcaaaga catcccgccg
      301 tgtttatggg cgatgcggcg aatatttcta gcgacatttt tagcaaagcc cttttcgcct
      361 tcgctccagc cgagaaagag acttctttcc atcggcttgt aaccgccgat tttaaatagt
      421 tttgccagcg gcgattcatc gacgccgtaa aggcgcatca tcaggtcggt ttcttcggcg
      481 tcggagcagc ggaagaccga agcaaagccg gcctcgcact gcatcatctc gcgcgtcgct
      541 tcctgagctt cttcccagtt atgaaacata aatgagaagc acttgcggtt ttccttggta
      601 agacgaaaga cgcgaagagt gacatcggtc aggacgccaa aggctccttc gctgcccatc
      661 atgatgtgat ttaaagaagg tcctgtcgct tcgcgcgggt aaggagaggt gacgatattt
      721 ccaattggag tcgcgtattt ttgctccagg accatatctt cgattttccc atagtaagtc
      781 gagttttgac ccgcgccgcg cgtgacgacc caacctccga cggagctgta ttcaaatgat
      841 tgaggaaagt ggccacaggt ataggctctt ttcgcaccaa ataacttctc ggcgtcgtta
      901 agggtttttt ctagttgcgg accgctcatg ccggcctgga cggtaatcgt ctgatctttt
      961 tcattaaaag cgatgacttt attaaaacga aggcgcatgt cgaggctgac accgccttta
     1021 acgcattcca ccccgcgtgt gacgctgctg ccgccgccgt agacataaac gggaatttta
     1081 tgagtcgagc aatacgcgat gactttttca atctgctcgc gattgtctgg atagaggacg
     1141 gcgtcgggga tgttttcgac gattttttgt cttaggcgca ggacatcgtg catcgttttg
     1201 ccgtaggcaa cgcttaaacg cgagtagcta tcggttctga caaactcttc acctagaaga
     1261 gccttaaaag catcgatgtg ctctggcgcg agacaagagg gcacattata gtcgacaggc
     1321 ccgaaaccta gacctccgtc atacttttga aaatcatcat ccgtgagatc aaaaatctcc
     1381 ttcatgacct tgtagagatt ttcttttggc cacttaaaat aatgcgggtc gccccagcga
     1441 aaaatagagc gccagctgcg cgggtcggca ggtgtttcaa cccatttcgg ggagaagcct
     1501 ttgtatggtt tgttagacat tatttgtcct tcctttcttt ggtgatggag cgaagttccc
     1561 aatagagggg ttttaactgc ttgtaaattc gtttataaat gcgggagtgc aagttcctat
     1621 agacttcgtg aacctctgga tttggcgtaa aggttttctt gtaatgcacc atgctctgaa
     1681 tcgcctcgcg gtagtcctta aagacgccga ggcttacaaa agcgaccatc gaactgccga
     1741 gtccgcacgc ctcataagtc tgaattcgat agacgggttt gccgaacata tcagcggtga
     1801 tctgacatat ttcgtcgctt tgcgaaccgc ctccggagac agttaaaccg gatatttttt
     1861 gtttggaact cttctcgaga ttttgcattc cctcgagcag tccgtagccg ataccttcga
     1921 tgatggcttt atagatgtga acgcgcgtat gttcgcttga gaacccgatg atgcatcctt
     1981 tggcttccgg ctgttttaag gtcggagccc aataaggctg aaggatgagg ccgtcacttc
     2041 ctggcggaat ttctttgagg cggcggttta acagctcttc gggtgaaagg ccgagtttgg
     2101 gagcttgaag aacttctttt tcagcgaatt cttttttaaa ccagctaatc atccagaagc
     2161 cgcgataaac ctgaatctca ggattccatg tgccgattgt cgcgccggga taggccggca
     2221 tgaattgctg cggttcgaca tatttgcgaa gcgtcgtctg tatcgtagcc gtcgttccga
     2281 agctgagcga tgccaaatca ttatcgagaa cgccgcagcc aagcgtttcg cacgccttgt
     2341 cagaggcggt ggcgatgacg ggcaaacctt ccgcgatcaa gtactcctca ctaacctctt
     2401 tggaaatggt gccgagagta gacccgggcg ggaggatatc ttgtaattta tcggtatcga
     2461 cattaaaaat cgcaaatttg atgtttgatg gttcatacca tttattagtt ttatagtcat
     2521 agggaatatg agcgacttgc gaagctgagg aatcgatgag ttttctcgtc agctggtaag
     2581 tcaaaaaacc gctgagtgtc aaatatttat aagtggcagc ccacacctca ggctcgtttt
     2641 cctgaatcca gttgctcttg gtaattctcc tttgatcttc gagagcctcg ctcatgccga
     2701 cgagcttaaa agccatccgc gccatcaagg gcagcggatt ttcacatttc gcttctcttt
     2761 ggtcaagcca gattatgatg tcgcgaagcg gcttgatgtc tttatcgacg cagacgcacg
     2821 tatcgcggat tgtcgtaatc gtcacgccga taatctgtgc gactttgtcg gggttatttt
     2881 gatgaatgcg ccgcgatact tctttaagcg tatcgatgta gacgctcggt ttctgttcgg
     2941 cgtatcctgg ctgcagagag tagtaaggaa tgaacgaaat ttgctctttg gccacgatat
     3001 taccttgttt gtcgacgagc agcgaccgaa tgctttgcgt tccacaatca aaggtcaaaa
     3061 cgagaggatt cataatcata agcacttatc tcctttttgc atattcgcgg ttgttatgac
     3121 attaacatct gttccaaaaa cattatggac gatgattgtt ttaattggga gatattccgt
     3181 gacaacaaca tcttttagaa gatgaatcaa gtccattatt ttgtctttgg cgatatcgcc
     3241 gtcggtccgc accgagatga ccggatattc aggaagagtc gtgcggacgg tggtggttat
     3301 gctccgctta ggatccgtca gttcattgat ggcaaaagca atgccgcgcg ggcatttgtt
     3361 gccgatgacc tcgtgcgact gcacatcgat ttcgaggcgg cagccgttcg gacagacgat
     3421 gcaggtcagg ctctttttca tggtttgacc tccagataaa cgcgaactgt ggcaggcgca
     3481 aggtcaggcg ataaagaaaa gacgattctc tccatctccg gagggcgcag gtgcaaatag
     3541 cgtttcttaa aaagcgtctt tccgtcatgt tcgatatata gagtcgcgtt ttcatattcg
     3601 ccggcacttc tgaaatatgc gattacctgg cgtgtcggac tcttaaaatc aagatgggag
     3661 ggaacgaagt acataaggtg cgaatcaatt tcgatttcct gcaaatctcg ttctttttga
     3721 atgcttttcg ccgcataagt gccggccaat tcgccgcttt ccgagacgta atcggcgagg
     3781 tcgctgacat gcaaagagtt gccgcatgag taaacatgcg gagcgaatgt catcagcgtt
     3841 tggtcgacat aagggccttt tgtctttggg ttgattgcaa tttttaattg ctcggcgatt
     3901 tcgttctcgg gaatgagccc gaccgagata attaatgcat cgcatgcgac gatgtgtttg
     3961 ctctcttcaa ttgcttgcat gtgttcatcg acctcggcga tttcgacagc ttcaagccga
     4021 tcgcaaccaa acacttttgt gacggtatgc gataggtgaa gaggaatatt gaaatcatca
     4081 agacactgaa cgatgttgcg cgttaagccg ctgggcgtcg gtttgacttc atagacgccg
     4141 agaacctgac cgccctcaag ggtgatgcgc cgcgccatga tgagcccgat atcgccgctg
     4201 ccgagaatga cgcagcgttt ggtcggcatc ttgcctagaa tatttatata gtattgagcc
     4261 gctcccgccg tcatgagacc cgagggattt gttccgccga taaaaatttg tcgtgatgtg
     4321 cgctcgcgac agcccgtggc caagattagt tttgtcccga tgacgcttgc aataccatcg
     4381 cgagttacca acgtaagcgt gaaactatca tcattttttt ctattttggt aacaaacgtt
     4441 aaggtattga cgctgacgcc agttttctta atcttttcga tttctcgatt tgcgtattcg
     4501 ggaccgctta atttttcgtt gaatcgcgtc aggccaaaac catcgtgaat acattgtttt
     4561 aaaattccgc ctaattttgc ttcgcgttca atcaaaagga cgctggctcc gttttcgtca
     4621 gcttttaggg cggccgccaa ccccgctggg cccgcgccga tgacaatcac atcatagcgt
     4681 ttcatctcat ccccccttta ttatcatcaa gaaggatggg ggcttcgcct tttttcttta
     4741 tttcggtcag caaagccttt tgctcttcag ccatgatttg gacgattaac tgctggcaaa
     4801 aaccgccctg acagcgaccc atgccggccc gaatccgcct tttgacgcca tcggttgtat
     4861 ggactttcag cgggcgcctc agacaatcga gaatctcgcc tttggatatt tcctcacagc
     4921 ggcagataat ctggccgtaa tcaggattgc ttttaattag cttatcgcgt tcctcgacac
     4981 ttaattcttt gaggagacga ttggatttac gcgttggatt aaattgcttt ttcggcgcta
     5041 atttttcacc gctcaccatc tcatatattt tgacgatgtc ttcagcgatg gccggcgcgg
     5101 cggtcaatcc cggcgactga atgccggcgg cgtggatgat attttctgtc cacttgccgc
     5161 gacggacgat aaaatcctct tcatatgtcg aggcgcgaat gccggcaaag taagtgatga
     5221 catcgctcct cgaaagactc ggtagtgtgt gacgatgttt atcaaagagg gcatcaatct
     5281 cttcctttgt cgtctgataa tcttcgcgtt ccgggcattc gctggccgtc ggtccgacga
     5341 cggtgtttcc atcgacggtg ggaataattc ctccaccttt agtgtgagcc tttttatcgc
     5401 tgtttccggc ataaaaagac agtgtgctgt taaccgattt tgacgctgct ttcttgtcga
     5461 gaatcatctc gacgccgcgg cgtggatgaa tggaaaaaaa ctgatcaccg gccatcccag
     5521 cgatgatgtc ggcaaagacg ccggcagcgt tgatgacgac gcgcggatag atgatgccgc
     5581 ggttcgtttt aacagaaatg atgcggtgat ctttaacttc aaatttcaaa gcggcggtgt
     5641 cgaggagaac acgcccgccg ttttcaatcg cgttttcggc gagagcaaca accatgttgt
     5701 aaggagaaac aactgctcct tcagggaaaa agacggcaaa cttggcctct ttgccaatgt
     5761 ttggctctct tttcaataat tcttcacgct ttagcaggcg gacgcctttg attccttttt
     5821 tccgagctgc cgaaaaaaga aagggcatgg cgagataggc gagagtttta gtgaatacca
     5881 gcgtttgccc gtgcttaaca taatcgacac ctaagtcctc ggctaattgc tcataggatt
     5941 ggacggctct cctcagatag tgatgcttct tcgtctttcg cgataagtcc atgccgacat
     6001 gaatgcaccc gtcattgcgg ctgctggcgt gcaaggcgac atcattttct ttttcgatta
     6061 aacaggcatc gacatcatat ttgctgaatt cgcgaagaat cgcggttccg acgatgcctc
     6121 cgccgacgat gagagcatcg cattttaagc catcgtattc cgaatcttgt attgagctga
     6181 ttcgcatggg ttcaggagta taacccttga gcttgatgtt attgatgacc ccttcacttt
     6241 tcgcttgtgc ggcaatgtag ccgactttga caacttcgtc ccacttttcc atctcgcctt
     6301 ctaaaagaag gcatccgtct ttgattgtgg tgcggacacc gggatagagg cgagagattt
     6361 cggcgatagt tttttcaaaa gtgggtttca tcatctattt cccccccacg atttggcgaa
     6421 ttgaacgagt cttttttcat caaatgaatc atcttttaaa atctcttgga cgacgaggcc
     6481 gtcaatcatc agcataagcg tataagcaaa agcctcgggg tcatcaactt ggcgaaatac
     6541 tttctcgatg ccgattaaaa gtgtcgcccg ccactcgcgg tatttttcga tgaatttgct
     6601 tctgaggaca tcgttttcgc cgaggcattc attaattagg aaaatgtgca tcttggcccg
     6661 gtcaaagagc ttgacgcttt tatgaaagac aacctcgaga aagcgttccg gaggcaggtc
     6721 gttttcgtgt cgatccagcc acaggatata ctcagttttc agggcatcga tatgctgtga
     6781 tatgacatca aagacaagtt catccttagt cgtatagtga taataaatcg ttcccggaga
     6841 aagacttgct tttagcgcca catcgcgcaa cgaaaagttc ttgaccccct ttgtagcgat
     6901 caaatcctcg acggcggcga tgatccgctc cttgcttttg cttcctttag gacgagccat
     6961 tatattgtta ttcggttaac tgaccgaatt actcctacta gtattttaac aagaatagaa
     7021 taaataaaga agaagcaatc gcataaaaaa gaccgcctca ttaagagacg atcttcgtgt
     7081 taattatgaa tgaaagttag acggttggtt cttcttcttg gggaatctcg gtgtaggtat
     7141 cgagattatc cggcaagacg acatcgacga cttggttcat attgtcgata tcaatatgga
     7201 agaaaccttc gagttcgtgc agttcatggt cgataacagg ttcttcagca cgcatgtaga
     7261 cgcccgtctc ttcttcttga gcaattgtca cggcgacatc gacatcgaca tagagtttgc
     7321 tgaggaaacc atccgcaccg acaccgatgg tcagttttgc ggttgtaata ttgatgactg
     7381 aagaaatcat ttcgcgcatt tgtgtgcgaa aagtctcaat ctcttcgctt gtcgcatcgg
     7441 cgggaatttc accagcatcc tgcattactt tgacaccgat attaacaatg tcatcgatgg
     7501 taactgtgta gacaacggtt aattcgccat ttgactcggt ggctttgaca tcgctaagca
     7561 gattggcgta tggaaccata tcatcgaatg cgaaaggcat ttcaatcggt tcttccggat
     7621 tgtagccgat ggcttcgcct aggttgccga catatgtttt gattgtcgta acttcaggag
     7681 cttcttggtc catcaatgcg aacaactcgg tggcatcggt gacatcggca tagacccagc
     7741 cttcgctgag atagacggcg acgccggcat cgacatcgac taaagtctcg tcattttcga
     7801 cagccaaaac ggaagctgaa gcggtgacgc tgccgacggc atcttcgcca aatatgtcat
     7861 tggccttgat gtgaacttcg ccagcggctt cgagattgcg ttcaaagagg acgacatcat
     7921 cggcatcaaa agcctttccg gaataatcca ggtccgccgt gatatcgatt tcgaacgatt
     7981 cgatttccgt ggtggtcatt tgctccatcg catccatgac gcgctgattg gcgaccgcgg
     8041 cttcaatggt gatgccgttt ccgtcgcacg aagtcatgac cgtcaaagcg aagagcgaga
     8101 gagcggagaa taatagttta ttgaatttgt tcatttgaac acctccaatt tttactatag
     8161 cactaactga aaaaaaacaa taaaaaaatg caataaacaa tgaggaggca tttattgatt
     8221 aaggataggg agagttataa cgaattaaat tattataatt taaagaacca cttaataaat
     8281 gattttttct tttcgttata aggcggatat tttagttgca gctccatttt ctgtttcttt
     8341 agaacacttt tttcatggct gaaggcctca aacgatttct gaccgtggta actgcccata
     8401 ccgcttcttc cgatgccgcc gaaaggtagc ttgtcgttag ttaaatgcat gatggtatcg
     8461 ttgatgcagc cgccaccgta actgactaaa tccatgatcc ggcgcgcttc tttttgattt
     8521 ttggtaaaga ggtaaaaagc taatggttta tcccgcgatt tgaatatttc gagcaagtcg
     8581 tcgaccttag taaaagtgag aatcggcata atcgggccaa agatttcctc gcccatgatg
     8641 gcgtcatcat aggtgacatt gtccataatc gtcggttcaa ttaagcgctt atctttattg
     8701 atatgcccgc cgaaaacaat tttgtttttg tcgattaatc ccgcaaggcg ttcgatatgt
     8761 ttatcgttaa taatactcgg aaaatcttcg ctgatattgc catcatggta gtagaaggct
     8821 tgaatgtatt cgatgacgcg agaaataaaa gtgtctttga ctgattcgtg aacatagatg
     8881 tggtcggggg caacgcaggt ttgcccggca ttgaggtact taccccacgt gatgcgttta
     8941 gccgccatat cgatatcggc gtcttccatc acgatgcaag gtgatttgcc accgagttca
     9001 aggatgacag gtgtcagatg aacgctcgcc ttctcgagaa ccacgcgacc gacatcgtcg
     9061 ccacccgtga aaaagataaa atcaaacttt tggttaagaa gagcctgatt ctcctctctt
     9121 cccccgacga ccacgctgaa gaggcgggga tcaaagttac tggcaatctt ttgcaagacc
     9181 agcgagacat tccaggcata tttcgacggt ttaagaacga cggtgttgcc ggccgcaagc
     9241 gcagcgacga cgggaaccat cgctagttgc aaaggataat tccagggcga cattactaaa
     9301 gtaacgccat agggttcttt atagatatag ccacttgaga caatattgac gaggctcgtc
     9361 gccacgcgtc ttggcttcat aaatttcttc agatgatgga gcatgaaatc aatctcttga
     9421 atgacgagag agtactcggt tgagataaca tcgaattcgc atttgttgta atccagttta
     9481 aaggcatcat aaatatcctg atgcatgtct agaaggattt ttctgagctt ccgcaggact
     9541 tcccggcgag cgtctaaaga gcgtgtgtga ccttcaaaat aataggcgcg ctgcttttgc
     9601 acaatttctt caatcatcgt tttttcctct atcttttaat tgtccatatt tttcattaat
     9661 ttgccacaag aggttgtaag catgacgttt agcaaagaag ccgatgaagg aatataatat
     9721 tttattatga acatttttta tctcgaccct gatccggtca tcgccgcccg ctaccacgcc
     9781 gacaaacatg tcatcaaaat gattctggaa tcggctcagt tcctctcgac gacgcaccac
     9841 atcgcgcaat cgagtcaaga gacgcttgac aatatttata agcccaccca ctttaatcat
     9901 ccaagtacga tttgggttcg tcaaagcaag gccaattatc tttggctttt ctctttgttt
     9961 ttggctctga ttgacgaata tgcgtttcgg tttggcaaaa ctcataaaag cgcagaacta
    10021 atcccctatc tagaaaaggc gccaaaggcc ttaagcgaca gcctcttttc acctccgacc
    10081 ccggcgatgg atgaagagta tgtggtcgat ggcgattcac ttttgtcata tcgcaactat
    10141 tatcgcctcg ctaaaagcca tcttttcgct tggaccaagc gcgagcggcc ggagtggctg
    10201 aaagactgag catgcttatg catgttctcc aagatagtct ttgatgatga cattaaataa
    10261 ggtaacattt aacaaggagc atttttatga acgaactgaa aacaattgac cgtgacattt
    10321 atgatgcgat cgaattagaa aagaagcggc agcgtgaaaa tatcgaactg atcgccagcg
    10381 aaaactttgt ctcgttggcc gttttggaag cccagggttc gattatgacg aataagtatg
    10441 cagagggtta tcctaatcgc cgttattacg gcggctgcga gttcgtcgat atcgccgagc
    10501 aactcgctat cgaacgcgta aaaaaactct ttggcgttaa tttcgccaat gtccagcccc
    10561 attccggatc gcaagccaat atggctgttt tccgggcctt gcttaatcac ggcgaccgca
    10621 tcctgagcat gtcgcttagc gacggcggac atttgacgca cggctataag ctctccttct
    10681 ccggagccga ctatgaaatc tactcctatg gagttgatgc cgttactgag atgataaatt
    10741 acgacgaaat tgagcgtctg gcgcttgaag tgaagcccaa gttaattctg gccggagcga
    10801 gcgcctatcc gcgggcgatt gattttaaac gctttcgcga aatagccgat aaagtcgggg
    10861 cttttctttt cgttgatatg gcccatatcg ccggcctggt cgccggtggt gttcatcaaa
    10921 atccctgccc ctatgcccat gtcgtatcat cgactacgca caagactctt cgcggtcctc
    10981 gcggcggcat tattttgagc aacgatgcgg aaattgccac taaaatcgat cgggccattt
    11041 tcccgggcat tcagggcggt cctctgatgc atatcatcgc cgctaaagcg gttgctttta
    11101 aagaagctct cgaaccttca tttaaagatt atgttaaaaa tattgttgat aatgctcacg
    11161 ccctcgccga tgagcttaaa aaactgggat acagaatcgt cagcggcggt agtgacaatc
    11221 acatgctcct cgtcgatttg aaagcctcga tcggcatgac cggcgctgaa gccgaaaaac
    11281 ttctccatga agtcggcatc acgtgtaata agaattctat tcccaacgat aaagaaaggc
    11341 cgatggttac ctccggtatt cgcctcggca ctccggcgat gactacgcgc ggttttacaa
    11401 gtcgggattt tcgccttgtc gccaccctta tcgacgcggc tttaaaaaac cgcgataatg
    11461 cggacaaact ggccgaaatc cgtcaaaaag tattagaatt aacaagggag catcctcttc
    11521 cttattaatg atgattaata ccgaatcgaa actgatttat cagatgattg ccatcgccat
    11581 gggcgtggct ctttcggttt tgctgacaag ctttgtctat ataccgcttc cgttcggcgg
    11641 attatttaat tttaccgatg ttttcatcgt cttatactcc atgttttttg ggccgtggat
    11701 cggcggggca gtcggcgcct tgagcggttt actagtcgat attatcttag gctataattt
    11761 ttatgcaccg tttacctttt ttatcaaact gattgaaggt ctgatcgtcg gctacttgtt
    11821 cttaaaaatg cctaaaaaaa ttcgctatgt cggccctttt atcggcggta tcgtgatggc
    11881 tgctctctac atcattcccg actttatcct catgcaaaac tggattgtgg ctttgacttc
    11941 ttttggtatt aacttacttc agggtttcct cggagcttct ttgggcattg gcattttcct
    12001 cgttttgatc aaagccaaag tccgtcctcc gacccgctca taaataagta tttataactc
    12061 gacaagtccg cggaatatgg tagaattatc cgcggactgt ttttattact tgtgaggtcg
    12121 attatggaaa caatacagga ttcaaaattt aaacgggcat cgcggcgcgg cgtcctggca
    12181 tttcagcacg tctttgctat gtttggggcc acgattgtcg tcccgcttct cgccgggcta
    12241 tccgtgccga tcggccttct ttcagccggt atcggaacca tcatctttta cttcttaacc
    12301 aagaaaaaag tgccggtctt tttgggcagt tccttttcat atctgccggg catcatcgcc
    12361 tatatggtta gccagaatgc cggcacggtg ggaaccgaat cgtggcagcg ggccgtcggc
    12421 ggactcgctc cagccatcgc cataacagga ctcgtctatg ttatcctttc catcatcgtc
    12481 aaatttgtcg gcatgcagcg aattaaaaaa ctctttccgc cggtagttgt cggcccgatt
    12541 attatcgtca tcggcatggt tttggctccg aaaattcttt ataacaatgt catcggtcaa
    12601 tacacaaacg gggcgatgga tgcctggaag gcatggacaa tcgctatcgt cacgctgctt
    12661 tcaattgttg ctgtctcgac ggcatcaaaa gaaaaaagtt tctttcgcgt gacgccgatt
    12721 ctcatcggct ttgccatcgg catcattacc actattacca tcgacatgat tgaactttat
    12781 gccctctcgg gcgatattgc taaaacgatt atcttttaca aatttagcag tgccggcgca
    12841 cttgccaacg gcattgactg gagtcagatt gtcatctttc aaaacattgg tcaaaccttt
    12901 ggtttttata agtatctgcg ccttgacttg aatgctgttt tgcttatttc tccaatcgct
    12961 tttgtcgctt tcatggaaca catcggcgac atcaacgcca gcagcgccgt ctgcggcaaa
    13021 gatttcactg ttgatcccgg catcaatcgc acggtattgg gcgatggtct ggcgacggtt
    13081 tcggcagcct tcttaggagc tcctccaaaa acgacatatg gtgagaacac ggctgttttg
    13141 gccatcaccc aaaactatga ccccttcaac ttgtttttag ccgccatctt cgctaccgtg
    13201 ctcggctttt tcaccatctt cggcaatttg acgctgacga ttccggcacc agttatcggc
    13261 ggagcttcca tcgtcttgtt cggcatgata tcggcctcgg gacttaaaac gctgatttcc
    13321 agtcgtatca acatgaacaa cacgaagaat atgatcgtcg tatcggtcat catgtcgctc
    13381 ggcctcggtc tagccgcttt atccctggtc ggtgatatca ccggcaataa tatttacaaa
    13441 atcgtcattg gcagcgtcga aatttcgccc ctggccgtca gcgctttggc cggagttatt
    13501 ttgaaccttg ttttgcccga ccgtcagaag gaaattgcga caatataata attagcgatt
    13561 gtttgttgcg aagaaattgt tttatcaagg tgtttcaact atcatttatt aataggcgat
    13621 taagccgaga acggaagagt gatttgctat gtgggatgca ttagagaata tagggacttg
    13681 gggtatcctt tttgctgttt taattatctt cctcgccaaa atcgtcgagg tcacaatcgg
    13741 cacgcttcgg caaattcttg tcgtgaaagg ctatcgcact ctcgccgtct tactcgctct
    13801 tgtcgaaatc tttttatggg tttttatcgc ctcaaaagta atcaacgatc tgagtgtcaa
    13861 cattttctat ggtatctcgt atggcctcgg ttttgccgcc ggtgtctatt tcggctcgat
    13921 catcgagcag aaattggctt tcggcatgct cctcgtccag acgataacaa ccgagaaaaa
    13981 aggtgtcgaa atcgctaatg cccttcgcga aaaaggttat ggagtgacga ctgtcgacgc
    14041 taaaggaaaa gttgatagcc gcaaaatctt aatggtttac gccaatcgcc gcggcagcca
    14101 ggaaatcgtc gagcaagtca tgctcatcga tccgacggcg atgattgtca caaacgatgt
    14161 cggaacgcta tccggcggat atattagaat taaaaaatca ctgctaaaat aaaatattta
    14221 gactaaaaat ggcgactaaa tggttagatc gcctttttta atccacccca gtttgcggaa
    14281 caataaatcg attaaaaaga cgagtccggc cggcgcgatg atcatcaaaa gcaaaacgcc
    14341 gagccacgcc atcaaagggt tatcgcccat ttcaagaatg gttgagagcg gtccaatcaa
    14401 ggcgcacgtt cccatgccgg ctccgaccgg tgtagtgtgt aacataaaga cggtggtggc
    14461 caatggcccg aggacggcag atacgataat cgtcggaagc cagattaatg gtttgcgcaa
    14521 gatattttta aattgcagca ttgatgtacc gatgccgact gaaatgacct tcccgacatt
    14581 attatcgcga atagacatca tcataaaacc gaccatctgt gtgcagcagc cgactgcggc
    14641 tgcccccccg gcgataccgc caagattgat ggcgacagca atcgccaccg aagagatggg
    14701 ggcggttaaa atcattccca tcaccgaaga gataacgacg cccatcaaga ggggttgata
    14761 agcggtggcc gtctgaatga actgaccgag aattgtcaca atccaagtca gtggaacggc
    14821 aaacagaaac gataaagcgg cggcgacagc ggcgataaat aaagggacga taatcaggtc
    14881 gaccgccgtt tgctttctaa agacgagacg atataattcg atgctgataa caacgaccag
    14941 ataagccatt aacggattgc cgtttgttga tgttccatta ataaaaggta atacatatgc
    15001 gccattgtcg atgcctaaaa ccatcgttga caccgcaccg gcgacaccgg cggagataat
    15061 ggcgattggt gacaagttct tgagggataa ggcgataccg ataccgatac cgatacccat
    15121 taatcctttg agaatattgc cgatgccgac gatgaactga atattactta acgtcccgat
    15181 ttgtccgatg ataaccccga taatcagcgt tgcaaacata ccgatagcca tgccgcccgt
    15241 cgtttttgtc aggtggtcca tgaagcggga aaaccatttt ttcccgggtt cagcgtccgg
    15301 tttctcgtta ttgagttctt gtttttcttg ttttgtcatc acgaattaat atatataaca
    15361 gacgataaaa cgcaatcatt ttcgatttcc cgaaatctcc cgtcgtgaca aactattaat
    15421 tattgcgcgg cgttatgata ttcttttatc gttgttatga accagaaaaa aagtctcttt
    15481 cgagaaatca tccgcttcat catcgtcggc gtcatcgcca cggttttgga ctatggcaca
    15541 tattctctgc tcgctttagc gattcccgac agttggagcc cgattcttga aaccgtcatc
    15601 tgcacgacca tcggatttat cgtcagcgtc atcgccaact acctcctgtc ggtcatctgg
    15661 gtctttcaga atgtcgatca aaaggccgac gtcaaatcaa aaaagaatat gtttctcttt
    15721 gtggtactct ctgctggtgg tctcttgtta ggaacagcgg tgatggtcgg ttttgaagcg
    15781 ttagcctcaa atgccctcca tgtcgatatc aataactgga ttagcgattt ccaaattaat
    15841 tatttgcggt cttctgcctt ctggctattc acactcttct tcggcatcaa aacgctcgtc
    15901 attttgtcgt ataattatat tacgaggaaa aagttgatat ttaagtcgcc aaaggagaat
    15961 gaagatgggc aaattacgga ttaacatgct atcgaaagcc gactcggtcg acggccaggg
    16021 cgtcggatcg gcttatcttg agcaagtcgc tctcgtcaaa gagtgtgatg acatttttaa
    16081 tgtaacagtt aatgagggcg gttcgaagtt tgatatcgtt cacgtccaca ctatcaaccc
    16141 gcagtattat ttgaagatga ataataagca tgttaatgtc tgctatgtcc acatgctgcc
    16201 cgacactcta gatggctcga tcaagcttcc taaaggcctc ttcaaattat ttaagcgcta
    16261 tgtcacaaag ttttatcgca aagccgatga aatcgtcgtc gtcaatccga tttttattaa
    16321 tcccctcgtc gatcttggca tcaaaaagga ccgcattacc tatattccga attatgtttc
    16381 aaaagaccaa tttcatgtca ttagcgaaga aaagcgcatg gaggtccgcg atttatatgg
    16441 cgttccccgc gaccggttcg ttgttctagg cgttggtcaa gtgcaaaccc gcaaaggcgt
    16501 tttggacttc gtggaaacag ctcgccgcaa ccctgagatg ttctttgtgt gggctggcgg
    16561 cttttcattt ggtaagatta ccgatggcta caaagagctg aaagaaatcg tcgataatcc
    16621 accggcgaac gtcaaattca ccggcattgt caaacgcgac aagatgaacg acatttttaa
    16681 catgtcagat gtcctgttta tgccctcgtt caacgagctt tttccgatgg ccatcctcga
    16741 ggccgtcaac gcccataaac ccgttcttct aagagacctt gatctctatg tagatattct
    16801 ctttgaaaag tatgaaaaag gccatggcgt cgatgatttt gttcaagtgt tgcgaaactt
    16861 aaaagccgat gcgaaaatct atcagaaagc ctcagaaaat tcggcataca tcagcgaatt
    16921 ttactccaaa gaacacgtcc gcgaaatatg gcgagaatac tatccgcgcg tctataagaa
    16981 atacgctggc ttaaaaaaat tcccc
//
LOCUS       DAOSSL010000020        16149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_3710_length_16149_cov_14.835032, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000020 DAOSSL010000000
VERSION     DAOSSL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 16149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16149
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_16149_cov_14.835032"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    <1..249
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            304..1479
                     /gene="trpB"
                     /locus_tag="PLH17_03000"
     CDS             304..1479
                     /gene="trpB"
                     /locus_tag="PLH17_03000"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOR20773.1"
                     /translation="MEFGKYGGQFVGGAALQAIKEVEAAYGRFKDDKAFQEEFKFYLK
                     EYANRPSLLYYAKNLSATLGGAKIYLKREDLNHTGAHKINNVLGQALLAKRMGKTKLI
                     AETGAGQHGVATATAAALFGLQCDIYMGRVDVLRQSMNVYRMKLLGARVIIVDDGLKT
                     LKEAVDSALSAWSQELETSYYLIGSAVGPHPYPTMVRDFQKVIGEEIQRQMQEQEGRL
                     PDYVIACVGGGSNAIGAFYDFIDKPQVSLIGVEAAGKGVDSKWHAATISKGREAVIHG
                     MNTLCLVEENGDISPVYSISAGLDYPGVGPEHAHLHEIKRVIYHSATDEEAVIAFEML
                     ARTEGIIPAIESAHALTEAIKLAPTLGKDKIIVVNLSGRGDKDVQQIASYRNSELLS"
     gene            complement(1489..2292)
                     /locus_tag="PLH17_03005"
     CDS             complement(1489..2292)
                     /locus_tag="PLH17_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR20774.1"
                     /translation="MKYALIAGACSGLAQAVIHSLKDDFIIFAIDINPIIHNLYEKDD
                     NIRAFICDITEPAQIKAVKMEIGKQTTSLDLLINFAGIVELGSLIEVPPAKFERSVRI
                     NVIGNYHVTHSFFPLIKNGKGRIIVISSEYGKLLGLPFHSFYTISKHALEIYADSLRR
                     EVKGLGIKVVTIRPGSFKTAMVDNIERQFKKLVDDTVLFKKPLSRMESMMKEEIKSAS
                     KPQKIIRTFHKAIYKKNPKIAYRVHNSLKMKLLNILPPQLQDWILSHFF"
     gene            complement(2416..2751)
                     /locus_tag="PLH17_03010"
     CDS             complement(2416..2751)
                     /locus_tag="PLH17_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20775.1"
                     /translation="MPEEKKPLFAQKSQEELLLNNRREFLAREFVYATPEFVEYGKKF
                     LPKKHHFMVLGDEIVYLSDDKLAVNEVFISEDPKMQITITALLEDKYVYDPFGTEKLQ
                     LECNKAKFK"
     gene            3160..3726
                     /locus_tag="PLH17_03015"
     CDS             3160..3726
                     /locus_tag="PLH17_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20776.1"
                     /translation="MSKILIVAGDLAAGKSNLARKLSAHLKVPYFSKEHIKEILGEEI
                     EYSKREDNKKLSNSSIDLLLHILERVTAVGESVILEANFHKEELEKVKQICEFNNSKV
                     VLLYLTGDIEALYDRFLYREMHKNRHPVHLTHPLRDVKEFEDYIHKWRSEEEVLPRNY
                     IDVSGCDRDIVFAKALETLNALEEKGSK"
     gene            3744..>5470
                     /locus_tag="PLH17_03020"
     CDS             3744..>5470
                     /locus_tag="PLH17_03020"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOR20777.1"
                     /translation="METEKIRNILFLGHQGSGKTTLVESLAGIINKTPKGAVENKNTI
                     SDYTDEEKARLSSCNLSVLSLDYNGYRLNLLDAPGNDDFVFDVIGVLDMVKGAVLVID
                     ATKRIEVGTIKHYNLLKKHGVPTLIFVNKMDKEMIDFESLLEEINQKLGKKAVSFVYP
                     IGHQEAFDGFINVVTLKARKFDGKQMIDDVIYDDKKEKILSLHNVIAEQVALTDEAML
                     EKFFGGETLSMEEIHRGLHAAVISGEIAPVLVGSAKKDIGILTMLEMLIDYLPNPAEL
                     KPYAGFDKDGKELIRKTIPSEPFSAYVFKTFFDQYKGAINVVKIASGTLNLGDTVLCP
                     NTQATMKISQMFTLLGNKQTAITTASAGDIIGLSKLDNVITGHTLCDKANPITYPRAK
                     YPTSVYYRAIAAKVSKDEEKLYSSLTKLQIEDPCLDVHRNNETKQLLMGGLSDSHLNY
                     VLERVKNIFSIQVDVAAPKIAYREAVKREATAPGRYIKQSGGSGFYGVVEMRFGPSGS
                     DANVFEETVFGGAVPKNYFPAVEKGFIESTEHGLLSGFPVIGIKAVLLDGKYHPVDSN
                     ELAFKMASI"
     assembly_gap    5471..5570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5631..6395
                     /locus_tag="PLH17_03025"
     CDS             5631..6395
                     /locus_tag="PLH17_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20778.1"
                     /translation="MKAKLINFFKEAWRPTLLIGAICLVIYLLSLLASGSTYFIFWGD
                     AANQAENNFPTLADVRDTQGGNFNWTFIFIFAVVIYVYVSELKKKNYRGIVAGLSLYG
                     VHWLYEIANAIIGKAAGAALWNVSPESTSFILLIGVSWELSMMFSLAGIIMSKLLPDD
                     PHKKILGINNRWLFAIGNAAFFAIFEIFLAGTSAFIWVYPWWGAIPVFITTYIPFFLA
                     AFLVPDAKVKTQRIFLLATWGLAVILLAVLIPAGII"
     gene            complement(6462..8438)
                     /locus_tag="PLH17_03030"
     CDS             complement(6462..8438)
                     /locus_tag="PLH17_03030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20779.1"
                     /translation="MPKIRKAYSTDIAFLKALEEQCFAPNRRFSFSSLLRSLSSTHQD
                     LYIIEVDDRPVGSATIFRFRKTWRLYSIAILPEYRHQSLGHVLMQHIIDLALGENIGH
                     LSLEVDSKDAATIEWYRSFGFRKIEVIKDYYASGEHADKMLLVLKENPKEKRYLANVA
                     IVDQKIDWLTRIPNLEVISAETFLNDTSYQYAKELRVFNFCTKLGYQTIGYYVSLLAS
                     ARGLRAIPNVATLEDATDETITKSLGDEVFDLIQESFKHSTRKEIVLRVIFGRTTPPR
                     FERLGKAMHRLFETPFLELKFVKQGNWLLSKIRILTVNDIEVDDYVIADAKHYFAHNR
                     YAISKIKQYKYDLAILVDPKEPAPPSGKTALDRFVEAAHKVGFYTEFITKADYSRVPQ
                     FDALFIRATTNVDNYTYQFSRYAYAEGLVVIDDPWSILRCANKLYFHECMKSENIKTP
                     KTMIVSSKTPLAKITGEFTFPIILKRPDSASSRGVFKVSDLDELKLKLRELFRLSALL
                     IAQEYIPSSFDWRIGILDGKALYACKYFMARNHWQIYNWNANKSRASVGPVATYLVED
                     APATVVDLALRAANAIGDGFYGVDVKEKDGQVYVIEVNDNPSIDHHWEDEILGEKLYL
                     QIMQNIMDRIEKVRQMSVNSRASDTKNSAKTPVN"
     gene            complement(8589..9029)
                     /locus_tag="PLH17_03035"
     CDS             complement(8589..9029)
                     /locus_tag="PLH17_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOR20780.1"
                     /translation="MTDSKWPKDLKKTRVRQAIVNVLREASSPLSALQITTEILKSND
                     KIWPSTLYRGLDALVSAELVRRIALTESSAALYELSTHRHSHYAICLDCRKMFAIHNC
                     PLTNYEPLIADKDFTIMNHKMEIYGYCGACLKKRIDKSNRERNQ"
     gene            complement(9022..9855)
                     /locus_tag="PLH17_03040"
     CDS             complement(9022..9855)
                     /locus_tag="PLH17_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOR20781.1"
                     /translation="MNIFAYEFMQRAFIVGIALAIIIPLIGLIMVLKRSSMIGDTLAH
                     TSLAGVAAGLLIGFNPIVGAIIACVIAVFAIEGIQKKLPKNKDLGMAVVLTIGISAAG
                     LLSTYIPSGVNFNSYLFGSITLVSQNDLLLIVSIAAVILLVFLVIVKDLYLMTLDEKM
                     AKLSGVKVSLINFIFTLLTAITVGIAAKTVGALIVSSLMILPAACAMQFEKGFYKTLL
                     LSVGFGVLYTVAGLVVAFYANQRAGATIVLIGAIIFVAVVFIKWIINKRRLERLRRAN
                     D"
     gene            complement(9855..10523)
                     /locus_tag="PLH17_03045"
     CDS             complement(9855..10523)
                     /locus_tag="PLH17_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOR20782.1"
                     /translation="MNVISIKDVTFSYETHHVLDHISLDIKKGDFLAILGSNGTGKTT
                     LLKILLNELKPTTGTVAIFGQDIKTFNDWTKIGYLSQNATANVAGFPTTVKELVASSL
                     HAELGVIKILKKSHRERVFEALKLVGMETYQNRLFAKLSGGQQQKVLLARSLVSRPEI
                     LILDEPTSALDEASSQALFSLLKKISLEKATTIIISTHSQACSNNNCNHIVNLDSERL
                     KEER"
     gene            complement(10525..11400)
                     /locus_tag="PLH17_03050"
     CDS             complement(10525..11400)
                     /locus_tag="PLH17_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOR20783.1"
                     /translation="MKKLLLYLPLITLILSGCNDSMTNDKIKIMTSFFVMEDFARKVG
                     GQYVDVQSIIPSGGEIHGYEPTTGDIVALSEADLFIYNGAGLEPFVESLQQAVGNPEL
                     QYIETSQGLTLLNEDDHQDAHTWLSPLNAKKQMENIKNAIVGIDAQNAAYYEERYSVF
                     ADKFDKLHEQYTVLLAPGAGNYLVTGHAAFGYLTDAYNLIALPIGGHDSEQEPTQQDI
                     ASVIDIVKENDIRYVYAESLNPSDAVLTIIAETGAELEILSPLEGLTTAQINSGEDYF
                     SIMKDNLVAIAKGFI"
     gene            11568..12584
                     /locus_tag="PLH17_03055"
     CDS             11568..12584
                     /locus_tag="PLH17_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR20784.1"
                     /translation="MKVFIVGGTGLIGSETARELISRGHQVTAIALPPLPKGALLPPE
                     MHIEYGNYMELNDEQLKKHLAGMDALVFAAGVDERIEGPAPIYDFFKKYNIDALERLI
                     KVAKEVGVKHVSICGSYFSYFAKKWPHLELTKHHPYIRSRIDQEKMALSYAEKGFDVS
                     ILELPYIFGAQPGRKPVWVFLVESIRKMKKSTLYTQGGTTMVTVKQVAQGIAGALETT
                     SGGVAYPFGFYNYEWKDFLKIVHKHMGYPRERKVVTIPNFLFALQGAQINKAHKKKGT
                     EGGLDMVKFTKLQTANLFIEPEEGALRLGVSEDDIDAAIGESVRLSLDILEAKTTDIV
                     DMKS"
     gene            12693..12896
                     /locus_tag="PLH17_03060"
     CDS             12693..12896
                     /locus_tag="PLH17_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOR20785.1"
                     /translation="MINEFGLDYVVHLLLLIFVGALWGGVIRIVRGKVLIGVLYILTG
                     AFFGIGWIIDLINMLTKKNFANG"
     gene            13022..15460
                     /locus_tag="PLH17_03065"
     CDS             13022..15460
                     /locus_tag="PLH17_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOR20786.1"
                     /translation="MIELKSITKDYLSSDNVTHALKGIDLNFRKCEFVSVLGASGCGK
                     TTMLNIIGGLDRYTEGDLLINGRSTKQYKGSDWDAYRNRSIGFVFQSYNLINHISVLA
                     NVELALTLSGVSAKERKARATEALIRVGLEDKLTKKPNQLSNGQMQRVAIARALINDP
                     EIVLADEPTGALDSVTSVQIMEILKEIAKDRLVIMVTHNPEIAEHYSTRIIRLHDGLV
                     TDDSNAYDGTTASPCQVNTDAVTKDYKKKTAMSFVTAFMLSLRNLATKKSRTILTSFA
                     GSIGIIGIALILSLSSGFSNYIYQVQVDTLSTYPITIEKETTDYWALMRGIRANDEDA
                     EEYPDSEVISSNPITSTFVSTVGEEAHENDLKSLKTHLETEEFKKKYEGDYSAIQYSY
                     DIEFRIYSSTYDNTSNSILLPLPFPPAFDVERYLNLRSIYSEMLNNVSLIEAQYELLA
                     GEYIDPEEEDDNHKVAIILDEYNRIPDYVLASLGLVSIEQLIIGTSGFSITFEDMMNL
                     RLKMPLAAELYDDVDDDGDYEGIRDNPGLMKNVLDDAIEIEVGAILRARPNISATSLS
                     GNIGYLSSLTREIIERTNQTPVVVAQRANEEIDVTTGIPFEGDVTIETRYQNFGVCDL
                     DNPDSINIYPTSFEAKDRLVAMIDDYNVDKSDSEKIKYTDYVAILMSSITLIINAITI
                     ILVAFVSISLVVSSIMIGIITYISVLERTKEIGILRSIGARKRDISRVFNAETLIIGG
                     FAGALGVGIAAILNWPVNLIINSYVDVGTISVLPWYAVFILIAISMALTLIAGLIPSR
                     IAANKDPVIALRTD"
     gene            15465..15980
                     /locus_tag="PLH17_03070"
     CDS             15465..15980
                     /locus_tag="PLH17_03070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOR20787.1"
                     /translation="MRIIGNYKDDQYEYKGVEKIRKIVRAVVLDEQNRIALTKINNTD
                     KFGIRDYYELPGGGVQKGETLLRALSREMEEEIGCEISVIKEIGKVIDYYNLILRENH
                     NYFYLVRVSNRVPSRLEPEEIERIEAIVWVPIDEAIRLYEKMQNILVGKLVKQRELPI
                     LQRAQKMINSL"
     gene            16025..16100
                     /locus_tag="PLH17_03075"
     tRNA            16025..16100
                     /locus_tag="PLH17_03075"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16058..16060,aa:His,seq:gtg)
BASE COUNT         4322 a         3638 c         3807 g         4282 t
ORIGIN      
        1 taaattcatt gcgaaagaag agtaacttag cccacttgca ggagcgagtc tatgacggtg
       61 aaagataggc gcaggatgac taagtgaatg gactttcaag aagcgcgtcg aaattaagta
      121 gccgcagccg gttgtcgccg ttatacgact taggatataa acgtcttaaa tgacggagta
      181 tctgattgag gagaaacgcg ataaagtttc ttgaaattcg ggtggcacca cgagcatctc
      241 gtcccgattg taagaggtgc tttttattac ctcttaaccc tcaaaagaaa gaaggatagg
      301 gtaatggaat ttggaaaata cggcggacaa tttgttggcg gagcggccct tcaggcgatt
      361 aaagaagtcg aagcggcata cgggcgtttc aaagatgaca aggcatttca agaggagttt
      421 aaattttatt taaaagaata tgctaatcgc ccatcgcttc tatattacgc taaaaatctc
      481 agcgccacac tgggcggtgc gaaaatttat ctcaaacgcg aagatttaaa ccataccgga
      541 gcccataaaa tcaataatgt tctcggccag gctctcctcg ccaagcggat gggcaaaacg
      601 aagttgatcg ccgaaactgg cgccgggcaa cacggcgtgg ccacggccac agcagcagct
      661 ctcttcggac tacaatgcga catttatatg ggccgcgtcg atgttttaag gcagagcatg
      721 aatgtctatc gcatgaagtt gctcggggcc cgcgtaatta tcgtcgatga tggacttaaa
      781 accttaaaag aagcggtgga cagtgcctta tcggcgtgga gtcaggaact tgaaacatcg
      841 tactatctca tcggttcggc ggtcgggccg catccttatc cgacgatggt ccgcgatttt
      901 caaaaagtta tcggtgaaga aattcaaagg cagatgcaag agcaagaagg gcggttgccc
      961 gattatgtta ttgcctgcgt cggtggaggt tcgaatgcca tcggtgcctt ctacgacttc
     1021 atcgataaac cgcaggtgtc gctaatcggt gtcgaagctg ccggcaaagg cgtggatagc
     1081 aaatggcacg ccgcgacgat cagcaaaggg cgagaggcgg ttattcacgg gatgaacact
     1141 ttgtgtttag tagaagaaaa cggcgatatc tcgccggttt attcaatctc ggccgggctt
     1201 gactatcccg gtgtcggtcc cgagcacgcc catcttcatg aaatcaaacg cgtcatctat
     1261 catagcgcga ccgacgagga agccgtaatc gcctttgaaa tgctggcaag gactgaaggc
     1321 atcatcccgg cgattgaatc ggcccacgcc ctcactgaag ctattaaact cgcgccgaca
     1381 ttaggaaaag acaaaataat cgtcgtcaat ttgtccggtc gcggcgataa ggacgttcag
     1441 caaattgcct cttatcgaaa tagcgaattg ctttcttaat gaaagaattt agaaaaaatg
     1501 gctcagaatc caatcctgaa gctgaggtgg caagatgttt aatagcttca tcttcagcga
     1561 attgtggacg cgataagcga tttttggatt tttcttatat atggctttat gaaatgtgcg
     1621 aatgatcttt tggggtttag aggcgctttt gatttcctct ttcatcattg attccatccg
     1681 cgaaagtggt ttcttaaaaa ggacggtatc atcgaccagt tttttgaatt ggcgttcgat
     1741 attgtcgacc atcgccgttt taaatgagcc ggggcgaatg gttacgacct ttattcccaa
     1801 gcctttgact tctcgtctca gcgaatcggc atatatttct aatgcgtgtt tggaaatggt
     1861 gtagaaagaa tggaaaggaa gacccagcaa ttttccgtat tcgctgctga tgacgatgat
     1921 gcggcctttt ccgtttttta taagcggaaa gaaagagtgg gtaacatgat agttgccgat
     1981 tacgttaatg cgcacgcttc gctcaaattt tgcgggcggg acctcgataa gtgaacctaa
     2041 ctcgacgatg ccggcgaaat tgatcagaag atcgagcgaa gtcgtctgtt ttccaatctc
     2101 catttttacg gctttgattt gcgctggctc ggtgatgtcg caaataaagg cgcggatgtt
     2161 atcgtctttt tcgtagaggt tatgaatgat ggggttgata tcgatggcaa agataataaa
     2221 atcatcttta agcgaatgga tgacagcttg agcaagaccg gaacaagccc cggcgataag
     2281 cgcgtatttc ataattatcc tcactaatct tatcattttg ggcagaggag atgtaaacag
     2341 ccttatattc gaacaaaaaa atccctattg tcgcattcga catcagggat taatttttta
     2401 tagattggca accgcttatt taaatttcgc tttattgcat tctaactgca acttttcggt
     2461 tccgaaagga tcgtagacgt atttatcctc aaggagggcg gtgatggtaa tctgcatctt
     2521 aggatcttcg ctgatgaata cttcgttaac ggcaagtttg tcatcgctga gatagacgat
     2581 ttcgtcgcct agaaccataa aatgatgctt cttggggaga aattttttgc cgtattcaac
     2641 gaactctgga gtggcataga caaattcccg agcgaggaat tcgcggcgat tgttgagcaa
     2701 caactcttcc tgacttttct gcgcgaataa aggtttcttt tcttctggca ttatctttcg
     2761 ccttctttct agtagcaacg acatcactac tataaattat aaaacatcgc tttttgcgag
     2821 taaattacat tcggtgaaaa ccaagcgaaa aatattacat atgtaaaacg agtttaattg
     2881 aagatgaagc gcttcgaaaa agaagattcc catagcgaca ttagggatga tgatgcctta
     2941 tatattattg gccgagacgc aaattagtta gattcattgc ctttgacggc cacaaacgat
     3001 tagccattgt cgatcgggat gggtttgcca ttcagcgcga tccaaattat ttatattata
     3061 tataacgata gcccattgaa gcatttgccc gttgtttctc ttattgatta tagatcgcat
     3121 aagattataa tatatatgta taatgcacaa ggagataata tgtccaaaat cctaattgtc
     3181 gctggcgatt tggctgccgg aaagtcaaat ctcgccagaa aactttcagc gcacctgaaa
     3241 gtgccctact ttagtaagga acacatcaag gaaattctcg gtgaagaaat cgagtattcc
     3301 aaacgggaag acaacaagaa gttatcaaac tcatcgatcg atcttctact tcatatcctc
     3361 gagcgggtga ccgcggttgg agaaagcgtc atacttgaag ctaatttcca taaggaagaa
     3421 ctagaaaagg tcaaacagat ctgcgagttt aataatagta aggttgtcct tctttattta
     3481 acgggcgata tcgaagctct ctacgaccgc ttcctctatc gcgaaatgca caaaaaccgt
     3541 caccccgtgc acctgactca tccccttcgc gacgttaaag aatttgaaga ttatattcac
     3601 aaatggcgga gtgaggaaga agttcttccc cgcaattaca tcgacgtttc cggctgcgac
     3661 cgcgatatcg tctttgccaa agctctcgag acacttaatg ccctggaaga aaagggctcg
     3721 aaatagaaca gaaaggagta tctatggaaa cagaaaaaat tcgcaatatc ctctttttgg
     3781 gccatcaggg aagcggcaag acaactttag ttgagtccct ggccggcatc ataaacaaga
     3841 cccccaaagg cgcggtggag aacaagaata ccatcagcga ctacaccgat gaagaaaaag
     3901 cgcgtctcag ctcctgcaac ctctcggttt tatctctgga ctacaatggc taccgcctca
     3961 atcttctcga cgcccccggc aatgacgact tcgtcttcga tgtcatcggc gttctcgaca
     4021 tggttaaagg cgcggtcctc gtcatcgatg ccaccaagcg catcgaagtc ggaacaatca
     4081 agcactataa tctcttgaag aaacatggcg tccccaccct catcttcgtc aataagatgg
     4141 acaaggaaat gatcgatttc gaatccttgc ttgaggaaat caatcagaag ctcggcaaaa
     4201 aagccgtgtc gtttgtctat ccgatcggtc atcaggaggc gtttgacggc tttatcaatg
     4261 tcgtcacctt aaaggcccgg aaattcgacg gtaagcagat gatcgatgac gtcatctatg
     4321 atgacaaaaa agagaaaatt ctctcgcttc acaacgtgat agccgaacaa gtggctctca
     4381 ccgacgaggc aatgcttgaa aaattcttcg gtggcgaaac gctttcgatg gaagaaattc
     4441 atcgcggtct tcacgccgcc gtcatcagtg gcgaaatcgc tccggtctta gtcggcagcg
     4501 ccaagaagga tatcggcatc ctcacgatgc tcgaaatgct gattgactat ctgcccaatc
     4561 cggcggaact aaaaccttat gccggctttg ataaagacgg aaaggaattg attcgcaaga
     4621 cgataccaag cgaacctttc agcgcctatg ttttcaaaac cttcttcgat caatataaag
     4681 gcgcgatcaa tgtcgtcaaa atcgcctctg gcacgctcaa tctcggcgat acggtcttgt
     4741 gcccgaacac gcaggcgacg atgaagatat cacagatgtt caccttgctc ggaaataagc
     4801 agacggcgat tacaacggcc tcggccggcg atatcatcgg tttatcgaag ctcgacaacg
     4861 tcatcaccgg acacactttg tgtgataaag ctaatcccat cacctatccg cgcgccaagt
     4921 atccgaccag cgtctattat cgggccatcg ccgcgaaggt gagcaaagat gaagaaaaac
     4981 tatacagctc gctcaccaaa ctgcaaatcg aagacccgtg ccttgatgtc catcgcaata
     5041 atgaaaccaa gcagctcttg atgggcggct taagcgattc gcacctcaac tatgttttgg
     5101 agcgcgtcaa aaatatcttc agcattcaag tcgatgttgc cgccccgaaa attgcctatc
     5161 gcgaagcggt caaacgcgaa gcgacagccc cgggccgcta tatcaagcaa tccggcggca
     5221 gcggtttcta tggcgtcgtc gagatgcgat tcgggccgag tggcagcgat gccaatgtct
     5281 ttgaagagac ggtcttcggc ggagccgttc cgaaaaacta tttcccggcc gttgaaaagg
     5341 gctttatcga atcgaccgaa cacggcttgc tctccggctt tccggtcatc ggcattaaag
     5401 ccgtcttgct cgacggcaaa tatcatcctg tcgactccaa cgaactggcc ttcaagatgg
     5461 cttcgatttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacgccaacg
     5581 agaaaaacga cagcaagtaa ttcaaaactg gttttaaaga gggttaatcc atgaaagcaa
     5641 aactcattaa cttttttaaa gaggcttggc ggccgacgct tttaatcggc gccatctgcc
     5701 tcgttatcta tcttctttca cttctggcta gcggaagcac ttacttcatt ttctggggcg
     5761 atgcagccaa tcaggccgaa aacaattttc cgaccctcgc cgatgttcgc gatacccaag
     5821 gcggaaattt taactggact ttcatcttca ttttcgccgt cgtcatctat gtttatgttt
     5881 ccgaattaaa aaagaagaac taccgcggca tcgtcgccgg actctctctt tacggcgtcc
     5941 attggctcta tgaaatcgct aatgccatca tcggcaaagc ggcgggagca gccttgtgga
     6001 atgtctcgcc ggagagcact tcgtttattt tgctgatcgg cgtttcatgg gagctttcaa
     6061 tgatgttttc cctcgccgga atcatcatgt caaaacttct tcctgatgac ccgcacaaga
     6121 agattctcgg tattaataac cgctggcttt tcgccatcgg caatgctgcc tttttcgcta
     6181 tttttgaaat cttcctggct ggcacatccg cctttatatg ggtatatccg tggtggggtg
     6241 caatccctgt tttcatcacg acttacattc cgttttttct ggccgccttt ttggttcctg
     6301 atgccaaagt caaaacccag cgcatcttcc ttctggcgac atggggtctt gccgtcatcc
     6361 ttctagccgt cttaattccg gccggcatca tctaaataag tatgaaaagg gattcgacaa
     6421 gtgcgccgaa tccttttttt atgcaaaata gccgcaaatc tttaattaac tggcgttttt
     6481 gcgctgtttt tggtatcact tgccctgctg tttacggaca tttgccggac tttttcaatc
     6541 cgatccatga tattttgcat gatttgcaaa tagagctttt cgccgagaat ttcatcttcc
     6601 cagtggtggt cgattgaggg attgtcattg acttcgatga catagacttg accatctttt
     6661 tctttgacat cgacgccata gaaaccatcg ccgatggcgt tggcggcgcg aagggccaga
     6721 tcgacgactg tggccggtgc atcttcaact aaataagtgg cgaccggtcc gacgctggcc
     6781 cggctcttgt tggcgttcca attatagatt tgccagtggt tgcgggccat gaaatatttg
     6841 caggcataaa gcgctttgcc gtcgagaatg ccgatgcgcc agtcaaacga actgggaata
     6901 tactcctggg cgatcaagag ggcgctgagg cgaaagagct ccctgagttt taattttaat
     6961 tcgtccaaat cagaaacctt aaagacgccg cgcgaggagg cgctgtccgg tctttttaag
     7021 atgatcggga aagtgaactc acccgtaatt tttgccagcg gagtttttga actgacaatc
     7081 atcgtcttag gcgtcttgat gttttcgctt ttcatgcact cgtggaaata tagcttgttg
     7141 gcgcagcgca agattgacca cggatcatcg atgacgacga gaccttcggc ataggcatag
     7201 cgcgaaaact ggtatgtgta gttatcgaca ttcgtcgtcg cgcgaataaa caaggcgtca
     7261 aactgcggga cgcgcgaata atcggccttg gtaataaatt cggtatagaa gccgactttg
     7321 tgggcggcct cgacgaagcg atcaagggct gttttacccg acggcggggc cggctctttg
     7381 gggtcgacga ggatggccag atcgtatttg tattgcttga tttttgaaat cgcatagcga
     7441 ttgtgggcga agtagtgctt ggcgtcggcg atgacgtagt cgtcgacttc gatgtcgtta
     7501 accgttaaaa tgcggatttt tgagaggagc caatttcctt gcttaacgaa tttgagttct
     7561 aaaaacggcg tttcaaaaag gcggtgcatc gctttgccaa gcctttcaaa gcgcggtggc
     7621 gtcgttcggc caaaaattac tcgcagaacg atttctttac gggtcgagtg tttaaatgac
     7681 tcctgaataa ggtcaaatac ttcatccccg agcgatttgg tgattgtttc atcggtggcg
     7741 tcttcaagcg tcgcgacatt aggaatggcg cgcaggccgc gcgccgacgc taaaagcgag
     7801 acgtaataac cgatcgtctg atagccgagt ttggtgcaga agttaaagac gcgcaactct
     7861 ttggcatatt gataactggt gtcgttaaga aaagtttcgg cactgatgac ttctaagttg
     7921 ggaatacgcg tcagccaatc aattttttga tcgacgatgg cgacattggc taagtagcgt
     7981 ttttctttgg ggttctcttt gagaacaaga agcattttat cggcatgttc gccacttgca
     8041 tagtaatcct tgatcacttc gattttgcga aagccaaatg agcggtacca ctcaattgtg
     8101 gccgcatctt tactatcgac ttcaaggctc aagtggccga tgttttcgcc gagggccaga
     8161 tcgatgatgt gctgcatgag aacatggccg agcgattgat ggcgatactc aggcaagatg
     8221 gcaatcgaat agagacgcca agtcttgcga aagcgaaaga tggtggcact tcccaccggt
     8281 cgatcatcaa cctcgatgat ataaaggtct tgatgggtgc ttgaaagact tcgcagcaaa
     8341 gaggaaaaag aaaaacgccg atttggagca aagcattgtt cctcgagcgc ttttaaaaag
     8401 gcgatgtcgg tcgaataggc ttttcgtatt tttggcatat gaccaggggt aatttaagta
     8461 aaggcggtgt cggcccccat ctcacctcga aaacacctat gaacattata tgctcatcct
     8521 aaaaaaagag gtgatgaaaa agaagtaaaa agatcgaatt ttaaaaatgc acctttatcg
     8581 gttgcatttt actgattcct ttcgcgattt gatttatcga tgcgcttttt aagacaagcc
     8641 ccgcagtaac cgtatatttc catcttgtga ttcatgattg taaagtcttt atccgcgatg
     8701 agcggttcgt aattagtgag tggacaattg tgaatcgcaa acatcttgcg gcagtcaagg
     8761 cagatggcgt aatggctgtg cctgtgggtg gaaagttcat aaagagcggc gctgctctcg
     8821 gtcagagcga tgcggcggac gagttcggcg ctgacgaggg catcaaggcc gcggtatagc
     8881 gtcgaaggcc agattttgtc gttacttttt aagatttcgg tcgtgatctg aagagctgaa
     8941 agcggcgaag aagcctcccg taaaacattg acaatcgctt gccgaacgcg cgtttttttt
     9001 aagtctttcg gccatttcga atcagtcatt cgctctcctt aatctttcga ggcgccgctt
     9061 attgataatc catttgataa agacgacggc aacaaatatt atagcaccga ttaaaacgat
     9121 ggttgcaccc gctctctggt tggcgtaaaa cgccacgacg agtcccgcga cggtgtaaag
     9181 gacgccgaag ccgaccgata aaaggagcgt cttatagaac cctttttcaa actgcatcgc
     9241 gcaggcggct ggaagaatca tcagcgatga gacgatcaaa gccccgaccg tcttggcggc
     9301 gatgccgacg gtgatggctg tcaagagggt gaagataaaa ttgataagcg agactttgac
     9361 gccggataat ttcgccattt tttcgtcgag ggtcattaga tataaatctt ttacaattac
     9421 gagaaagacg agcaggatga cagcggcgat cgaaacgatg agcaacaggt cattttgact
     9481 gacgagagtg atgcttccaa acagatagct gttaaagttt acgccactcg gaatataggt
     9541 gctcaatagt ccagccgccg agatgccgat ggtgaggacg acggccatgc ccagatcctt
     9601 gtttttgggc aatttcttct gaatgccttc gatggcaaag acggcgatga cgcaggcgat
     9661 gatcgctccg acgatgggat taaagccgat gagcagaccg gccgccacgc ccgccagtga
     9721 tgtgtgagcc agcgtatcgc cgatcatcga cgagcgtttt aaaaccatga tcaagccgat
     9781 taaaggaatt attatcgcta aagcaattcc gacgataaat gcccgctgca tgaattcata
     9841 ggcaaagata ttcatcatcg ctcctcctta agccgttcac tatccaaatt gacaatgtga
     9901 ttgcagttgt tgttggagca cgcctggctg tgcgttgaga tgataatcgt cgtcgccttt
     9961 tcaagactga tcttttttaa caaggaaaag agcgcctgcg aagatgcctc atcaagggcg
    10021 cttgtcggtt catcgagaat gagaatctcg gggcggctga caagacttcg ggccagtaaa
    10081 actttttgct gctgcccgcc cgataatttg gcgaacagac gattttgata agtctccatg
    10141 ccaactaatt tcaaagcctc aaaaacgcgt tcgcgatgac tttttttaag tatctttatt
    10201 acacctaatt ccgcgtgcaa actagaggcc accaactctt tgactgtcgt tgggaaaccg
    10261 gcgacgttgg cggtggcgtt ttgcgacaaa tagccaattt tcgtccagtc attaaaggtc
    10321 ttgatgtcct gaccgaagat ggcgacggtt ccagtcgtcg gtttaagttc attgagtaag
    10381 atttttaaaa gcgttgtttt accggttccg tttgatccga ggatggctaa aaaatcacct
    10441 ttcttaatat ctaaagagat atggtcgagg acgtgatgcg tctcatagga aaatgtgaca
    10501 tctttgattg agataacatt catatcaaat aaacccttta gcaatggcta ccagattatc
    10561 tttcattatt gaaaaatagt cctctccgct attgatttgt gcggtggtca gcccttcgag
    10621 gggactgagt atctcgagtt cggctcctgt ttcggcgatg atcgttaaaa cggcatcgga
    10681 gggattaagc gattcggcat agacatagcg aatgtcattt tccttgacga tatcgatgac
    10741 gctagcgata tcctgctgcg tcggttcctg ttcgctatca tgcccgccga tgggaagggc
    10801 gatgagattg taggcatcgg tcaaatagcc aaaagcggcg tggccggtga cgaggtaatt
    10861 accggcgccc ggcgccaata aaacagtata ttgttcgtgt agcttatcga atttgtcagc
    10921 aaaaaccgaa tagcgttctt cgtaataagc ggcattttgc gcatcgatgc cgacgatggc
    10981 gtttttaatg ttttccatct gctttttggc atttaatgga ctgagccatg tatgcgcatc
    11041 ctgatgatca tcttcgttta gtaaagtaag gccctgactt gtttcaatat attgaagttc
    11101 aggattgccg acggcttgct ggagactctc aacaaaaggt tcaaggccgg caccgttata
    11161 gataaataga tcggcttcgc tgagagcgac gatatcgccg gtggtcggct cgtaaccgtg
    11221 tatttctcct ccggacggga tgatactttg gacatcgaca tattgaccgc cgactttgcg
    11281 ggcaaaatct tccatcacaa aaaaactggt catgatcttg attttatcgt tcgtcatcga
    11341 atcattgcac cctgataaaa taagggttat aagcggaaga tataagagta attttttcat
    11401 ctttaaatgg taatgcgaat cattcgcaat tactacaata atacgcattt tcgttttcgt
    11461 caatcaaatc acttgcccgg tgagagcact tttctatgtt attagttaca tcttttataa
    11521 aataaaatta caagaacttt taccgtctta atttaaggag aaacaccatg aaagtattta
    11581 ttgtcggcgg aaccggcctc atcggaagcg aaacagcccg tgaactcatc agccgcggcc
    11641 atcaggtgac cgcgatcgct ttaccgcctt tacccaaagg cgcgctttta cctccggaga
    11701 tgcacatcga gtacggaaac tatatggaat taaacgatga acaactcaaa aaacatcttg
    11761 ccggcatgga tgcgctcgtt tttgcggccg gcgtcgatga acgcatcgaa ggtccggcac
    11821 ctatctatga tttctttaaa aaatacaata ttgatgccct tgagagattg attaaagtcg
    11881 caaaagaggt cggtgtcaaa cacgtcagca tctgcggttc gtatttctcg tatttcgcca
    11941 agaaatggcc ccatcttgaa ctgacgaaac atcatcccta tatccgctcg cgcatcgacc
    12001 aagagaaaat ggctctttca tatgccgaaa aaggtttcga tgtctcaatt cttgaacttc
    12061 cctatatctt tggcgcgcag ccgggccgca agccggtctg ggttttcctc gtcgaatcga
    12121 ttcgcaagat gaagaaaagt accctctaca ctcagggagg gacgacgatg gtgacagtca
    12181 aacaagtcgc tcaaggtatc gccggggcgc tggaaacgac tagcggcgga gtcgcttatc
    12241 cgtttggctt ttataattac gaatggaaag actttctaaa aatcgtccac aagcacatgg
    12301 gttatcctcg cgagcggaaa gtcgtaacaa ttccgaattt cctttttgct ctccaaggcg
    12361 cgcaaattaa taaagcgcac aagaagaaag gcacggaggg cggtctcgat atggtaaaat
    12421 ttactaagct gcaaactgcc aatctcttta tcgaaccgga agaaggagcc ttgcgcctcg
    12481 gtgtcagcga agacgatatc gatgccgcaa tcggcgaatc ggtgcgcctt tcccttgata
    12541 ttctcgaggc gaaaactacc gatatcgtcg acatgaaaag ctaaacttat cggcaaaaaa
    12601 gcaagtctta ttatttacac ttgttaaaat gttagtataa taaaattata agcgagtgct
    12661 tatgccttat tcatgggcca ggaggatttt taatgattaa tgaatttgga ctagactatg
    12721 tcgtgcacct tcttctcttg atctttgtcg gagctttatg gggcggcgtc atccgcatcg
    12781 tccgcggcaa agttctcatc ggtgttctct acatcttaac aggtgctttc ttcggaatcg
    12841 gctggatcat cgacttgatt aacatgctga ccaagaaaaa tttcgccaac ggttagtcct
    12901 gaacaaaaag aaaagaatcg tacatgcttg ggcatgtact ttttttatct ttttttgatt
    12961 actttagtta ttattgcgac ttgatgttgc tataattatt acgcattaac gaggtgagtt
    13021 tatgattgaa ttaaaaagca ttaccaaaga ttatttaagc agtgataatg tcacgcatgc
    13081 cctgaaaggc atcgatttga actttcgcaa gtgcgagttc gtctcggttt tgggcgcttc
    13141 tggctgcggc aagacgacga tgctcaacat catcggcgga ctcgaccgct atacggaggg
    13201 cgatttgctc atcaacggtc gctcgaccaa acaatataaa ggcagcgact gggatgccta
    13261 ccgcaaccgc tcaatcggct ttgtctttca aagttataac ctcattaatc atatttcagt
    13321 tttagctaat gttgagctcg ccctcactct ttcgggtgtg tcggccaagg agagaaaagc
    13381 gcgcgcgaca gaggctttaa ttcgtgtcgg tcttgaagat aaactcacca aaaaacccaa
    13441 tcagctgagc aatggtcaga tgcagagagt ggcgattgcc cgagccttga ttaacgatcc
    13501 ggaaatcgtc ttggccgatg aacccaccgg cgccctcgac agcgtcactt ccgtgcaaat
    13561 catggagatt ttaaaagaaa ttgctaagga tcgcctcgtg atcatggtta cgcataatcc
    13621 ggaaattgcc gaacattatt cgacgcgcat cattcgcctc catgatggtt tggtcaccga
    13681 tgatagcaac gcttatgacg gaacgaccgc ctccccttgc caggtaaata ctgacgcggt
    13741 cactaaagat tataaaaaga agacggccat gtctttcgtg acggctttta tgctttcgct
    13801 aagaaacctg gccaccaaga agtcgcggac aatcctgacc tcgtttgctg gttcaatcgg
    13861 catcatcggc attgccctta tcctctcttt gtcgtcaggc ttcagcaact atatatatca
    13921 ggttcaagtc gatacattgt cgacctatcc aatcacgatt gaaaaagaaa cgaccgatta
    13981 ctgggcgctt atgcgtggta ttcgtgccaa cgatgaagat gccgaggaat atcctgattc
    14041 ggaagtcatc tcttctaatc ccatcacttc tacttttgtc tcgaccgtcg gcgaagaagc
    14101 gcacgaaaac gatttaaaat ctttaaagac gcaccttgaa accgaagagt ttaaaaagaa
    14161 gtatgaaggt gactattcag caattcagta ttcttatgat atcgaatttc ggatttattc
    14221 ttcgacttat gacaatactt caaacagcat tttattaccg cttcctttcc cgccggcttt
    14281 cgatgtcgag cgctatctta atcttcggtc gatttatagc gagatgctaa ataatgtcag
    14341 tcttatcgag gcccagtatg aattattagc gggcgaatac atcgatccag aagaggaaga
    14401 cgacaatcac aaagtggcga taatcctcga tgaatacaat cgcatacctg attatgtttt
    14461 agctagttta gggctcgtca gcatcgagca gttaatcatc ggcacaagcg gtttttcaat
    14521 cacctttgaa gacatgatga atctgcgctt aaaaatgccg ttggccgccg agctctatga
    14581 cgatgtcgat gacgacggcg attacgaagg gataagggac aatcccggat taatgaaaaa
    14641 tgtcctcgat gatgccatcg aaatcgaagt gggcgccatt ttgcgcgctc gccctaatat
    14701 cagcgcgact tcgctttctg gaaatattgg ctatctcagt tctctcactc gcgaaattat
    14761 cgagcgaact aatcaaaccc cggttgtcgt cgctcaaaga gcgaatgaag aaattgatgt
    14821 cacgactgga attccttttg agggagatgt cactattgag acccgctatc agaactttgg
    14881 cgtgtgcgat ctcgataatc ctgattcaat caacatctat ccgacgagtt ttgaagccaa
    14941 agatcggctg gtggcgatga tcgatgatta caacgtcgat aaaagcgaca gcgaaaaaat
    15001 aaaatacacc gattatgtcg ccattttaat gtcctcgatt acgcttatta tcaatgcgat
    15061 tacgattatc cttgtcgcct ttgtttcaat ctctcttgtc gtgtcttcaa tcatgattgg
    15121 catcatcacc tatatctcgg ttctcgaaag gactaaagag attggcatct tgcgaagtat
    15181 cggagcccga aagcgcgata tctcgcgagt gtttaatgcg gagacgctca tcatcggcgg
    15241 gtttgcaggc gcgctaggcg tcggtatcgc cgcaatttta aactggccgg tcaatttgat
    15301 tatcaatagt tatgttgatg tcggaacgat ttccgtcttg ccatggtatg cggttttcat
    15361 tttaatcgcc atctccatgg ccctgactct gatcgccgga ttaattccct cgcgcatcgc
    15421 cgcgaataaa gacccagtca ttgcactacg gacggactaa tcgcatgaga attatcggaa
    15481 actacaagga tgaccaatac gaatacaagg gtgtcgagaa aattcgcaaa attgtccgcg
    15541 ccgtcgtttt agacgagcaa aaccgcatcg ctctcaccaa gattaataac accgataaat
    15601 tcggcatacg cgattactat gaactccccg gcggcggcgt gcagaagggc gagaccttgc
    15661 tgcgggcact gtctcgcgag atggaagaag aaattggctg cgagataagc gtcattaaag
    15721 aaatcggaaa agttatcgat tactataatc tcatccttcg cgagaatcac aattatttct
    15781 atcttgtccg cgtttcaaac cgcgttccga gccgccttga acccgaggaa atcgagcgca
    15841 ttgaagcgat tgtctgggtg ccgattgacg aagccattcg tttatacgaa aagatgcaaa
    15901 atatcctcgt cggaaaattg gttaagcaaa gggaacttcc aatcttgcaa agagctcaaa
    15961 aaatgattaa tagtctttga caacttcgca ttgtctatat atcattattt agcgtagtta
    16021 actggcgatc gtggtgaagt ggttaacaca tctggctgtg aaccagacat tcgtgggttc
    16081 gattcccatc gttcgcccca tatgacaaaa acactcgatt aaatcgagtt ttttatttct
    16141 acgggtatc
//
LOCUS       DAOSSL010000021        14439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_4265_length_14439_cov_17.973929, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000021 DAOSSL010000000
VERSION     DAOSSL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4265_length_14439_cov_17.973929"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLH17_03080"
     CDS             complement(<1..386)
                     /locus_tag="PLH17_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOR20788.1"
                     /translation="MRNRKGELVLHDPFFDDFFPLDLFKTDWPDIRRHTTMKTDIVAK
                     DNHYELMVDLPGLAKENVEISLENGYLSIKAAQKGETNDEKENYIHRERYYQSECRSF
                     YVGDGVEEKDIAAKMENGVLTITVPK"
     gene            complement(498..2276)
                     /gene="glmS"
                     /locus_tag="PLH17_03085"
     CDS             complement(498..2276)
                     /gene="glmS"
                     /locus_tag="PLH17_03085"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOR20789.1"
                     /translation="MCGIVGGVSNKPIRNYLIEGLKTIEYRGYDSAGIAVFNGKKAEI
                     YKVRGRVEDLDNKLEKRIDGLVGIGHTRWATHGEPSVINAHPHASKSGLFSIVHNGVI
                     ENFRAVKNALMEKGYSFLSETDTEVIANVIEENYRHTGDVYKAISKMMSDVKGSYALA
                     ILFNKEPDIIYFAKNRSPLLIGRGQNGNFLASDYLPMLKVADQFVTISDYQYGSISLD
                     DIKMFGRNDHPLPIVFHETELKPEALDLNGYPHYMLKEIEEADSCVKLLVDNYYDGKS
                     FLFDKEMMRQIKEADNIVFLACGTSYHACLLGSRFFRKAGKISDVYIASEWAYNPYTS
                     GKKTVYIFMSQSGETADLIHCLSVAKKMDGTIITITNTQGSTLSRSADHTLLLYAGIE
                     VAVASTKAYVAQVSALALLAGALNDDASVIADLEAVIAAQQSVIDNHDKIKNLASFVS
                     GKNDVYFLGRGLDYDLALECSLKLKEITYIHSEAFPGGELKHGPIALIEKDTPVIGFI
                     SDAKTELAMRSNLHEVEARGARVIVVSSITLAHPEDDFVVPYAPKYLSSLVKVLFGQY
                     LAYYVALKRGLNIDKPRNLAKSVTVE"
     gene            complement(2301..4382)
                     /locus_tag="PLH17_03090"
     CDS             complement(2301..4382)
                     /locus_tag="PLH17_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOR20790.1"
                     /translation="MEKQVVIEEFAKKQFNEKVMRSSLPLSVFKKYQQALCHETEIDR
                     ETADAIAHAMKIWAIDLGATHFCHWFQPMTGLTAEKHESFIQMDGEGEPIARLSGKSL
                     LKGETDGSSFPNGGLRTAFEARGYTYLDITSYAFVRGHVLFIPCVFVSYTGAKLDQKL
                     PLLVALEKMNVNASKILRKLGYDGFKSVKAMIGLEQEYFLVDKAQALMRPDIVFTGKT
                     LFGESMPKDQGMNDHYFGALTERVSSFMKEVNQKCWEVGIYASIEHNEVAPGQYEFVS
                     IYNNVNVAVDQNMVVMDILKRMALRHDLICLLNEKPFDGVNGSGKHNNFSLMTDTGMN
                     LFELGEDRSSNLRFLVFATAFIQAVDRYATLIRMACSDAGNDYRLGGHEAPPAIVSVF
                     LGDHLHRVFEELANRPNVTPLEFEKLYSPINNLSALPLDITDRNRTSPVSFTGNKFEI
                     RILGSSRNPAALNTVLLAGIAEALGEIDLKLKDIEPEKLSEGIIKLAHEILSRHHRVL
                     FAGDGYKKEWAEEAARRGLENYPHYIDSVETLKTPLVRRMLSSLNVLSEAEIDARYEI
                     YYTEFIETVLTQGKTLVSMVDKGIIPALIKTAALYKSTAIAMNGSATAIAKRSAECGR
                     LIDELDRHNELLSAGIKLASRNHSKKEKAEMCVHSIRPLLQEIKDLCNQAERILPKEY
                     YPYPTLSDLIV"
     gene            4819..5691
                     /gene="amrS"
                     /locus_tag="PLH17_03095"
     CDS             4819..5691
                     /gene="amrS"
                     /locus_tag="PLH17_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOR20791.1"
                     /translation="MKESLLYDKLPKGKVRCNVCGHHCLIVPGYRGICRVRSNINGVL
                     YSLNYGLCIAAAIDPIEKKPIYHFWPRTETYSFAAVGCNMHCPWCQNYEISQTPSNEY
                     VVEGDEVTPGEHVARALKFMTPSISYTYSEPTVFFEYALETMKLAREKGLKNIWVSNG
                     FMSEEMLREILPYLDAANIDIKGPDDEFYAKHCGGNLKTIINNLKTMQKANIHIELTT
                     LFIPGLNDEEYQIKKLVAAIVENLGLEVPWHISRFFPAWRMLNAHVTPLETLHLARSI
                     AQKAGLKYIYIGNV"
     gene            5693..7078
                     /gene="amrA"
                     /locus_tag="PLH17_03100"
     CDS             5693..7078
                     /gene="amrA"
                     /locus_tag="PLH17_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOR20792.1"
                     /translation="MNNLRAYFVPHPPLIIPEIGRGDEQAIKATIAAYQQVAKEIAAY
                     KPDTIVIISPHALTYEDYFHIEGGLSSRGNFADYKAPQISFNVAHDVDYVKKLASFAK
                     KTTFPAGTDGRANNPLDHATMIPLYFINQHYREYQVVRLSFSGLSFLTHYNYGKMIKE
                     IIRSDATKKYAVIASGDLSHMLKSDGPYGYVEEGPLFDKAVCKVLKSGNFLDLFNLDP
                     QMVEGAGECGFRSLLILAGTLDKLAVSAKLLSYEGPFGVGYAVASFEVINSDEKRNYG
                     AQHEEKEEKKLKLIRRKEDEYVSLARQTLELYVKTGQVLSIDEASDLLKRTRAGVFVS
                     LKKHGVLRGCIGTIQPTTKSIAHEIIQNAISSGTEDPRFPPVSISELSDLVYSVDILK
                     EPEPISSKNELDIKKYGVIVRCRGRSGLLLPNIEGVHSVEEQISIALSKAGISKHELY
                     TLERFEVVRHQ"
     gene            complement(7135..7812)
                     /locus_tag="PLH17_03105"
     CDS             complement(7135..7812)
                     /locus_tag="PLH17_03105"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOR20793.1"
                     /translation="MEENVYRMPLIGDKAPAFTAVTTQGMINFPEDYKGKWVILFSHP
                     ADFTPVCTTEFMTFASLADDFKALNTELVGLSVDSLYAHIAWLRKIKEIEWKNFKDIE
                     VKFPLIEDIRMDVAKKYGMIQPGQSNTQAVRAVFVVDPNGIVRTILYYPLSMGRNFDD
                     IKRIIQALQKADTDKVATPADWRPGDDVIIPTAGSCGTAKERMEDTSPDKYCLDWFMC
                     FKREGKK"
     gene            complement(7909..9180)
                     /locus_tag="PLH17_03110"
     CDS             complement(7909..9180)
                     /locus_tag="PLH17_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20794.1"
                     /translation="MANKWQKLLLAAAAVFVLSASGGDIAPQEVRRAAEAPLEVKLHF
                     FYSESCNYCSQEKVFLSSLAGDSQYDNVTIVSYLIDANLKTGGANPDYAVNMQLLEDI
                     AEVFDFSVGVPTTALGGKAFSGYDVTVRYFITQSIERYSQTPQVDVVSKIIEQGVASI
                     EPGDLDSTELEVELPFLGVIDPKAISLGLVSVVLGFVDGFNPCAMWVLLFLISLLLPN
                     NDRKRIFILGGIFILTSALFYFALMMAWINTAVFIAGNLAFRIIVGTFALAAGGYNLY
                     QFIKNIRQKDVGCETTDEKQRTKLMDRVRKIITQNKLILALLGVISLALIVNFIELAC
                     SAGVPLIFAYILALNDLGGWSGVGYVLLYIFFFVIDDIIVFTIVMLTLKIKVVSNKIA
                     RYNHLVGGILMLVIGILMIFFPTILQFNFLS"
     gene            complement(9170..9439)
                     /locus_tag="PLH17_03115"
     CDS             complement(9170..9439)
                     /locus_tag="PLH17_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOR20795.1"
                     /translation="MKAVIISAVWCPSCLIMIGRYHELREKYPDIEFLEYDFDADKEA
                     VAEHKVWKTLPVLILYQEGIEVARFIGEKSLKEMTLKIEALLNGK"
     gene            complement(9436..10548)
                     /locus_tag="PLH17_03120"
     CDS             complement(9436..10548)
                     /locus_tag="PLH17_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOR20796.1"
                     /translation="MIYLDYSATTKTDSRVLKYFNVVSDKYFANANSRYKLGRMSKSA
                     IKKAGRQILNTLGFNDHEIIYTSCATEANNLAIKGAIEALKGTKNRVITTSFEHSSII
                     APINALQRKGLLVSIVRIDSHGQVDLTHLQELMNPDVGLVSIGSVNSEIGIRQPIEEI
                     AKIAHASGALFHCDATQSIGKEIVPFHVADLVTFSAHKFYGIKGIGALIKRRGVALDA
                     QLHGGSSTTIYRAGTPNTPLILSLWKALFQVYKKHEQKYAKVKSLNTYLRELLTKIPT
                     VVINSPSEALPHILNFSLLGHSSRRVVEILSRQEIYISNHSACSSNTRKSLAVLALTG
                     DEKRALSSLRVSLSPSTTKQEIRTFVSALKKAGNSR"
     gene            complement(10648..11478)
                     /locus_tag="PLH17_03125"
     CDS             complement(10648..11478)
                     /locus_tag="PLH17_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOR20797.1"
                     /translation="MEKDCTHDCSTCGETCDERTDEKVDFSAKLNEHSSVKKVIGVIS
                     GKGGVGKSIVTAMLAVLMKRLDHNVAILDADITGPSIPKLFGLKERAYANDLGVIPLA
                     SKTGIDVISINLLLDNVTDPVIWRGPIIGNMVKTFWSDVVWKDIDFMFIDMPPGTGDV
                     ALTTFQSLPVDGIVVVTSPQELASLIVSKAVKMAQMMNIPIIGIVENMSYFRCPDNGK
                     DYQIFGASHIEDIASEYNLKVLAKIPIDPHLSSLSDLGLIELFKGSWLDKVPEILEKL
                     "
     gene            complement(11612..12274)
                     /locus_tag="PLH17_03130"
     CDS             complement(11612..12274)
                     /locus_tag="PLH17_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR20798.1"
                     /translation="MIKYYKNLLLTLGEIKGHPALLVHALTGCSFRCFHCFNFKELIE
                     KSHDEFYLIGDVIKYVVRQKDLYEYIVFSGGEYLDAAIDDLRSDLKQVRKVCDKPIII
                     YTNGTNHEKMKTLFEEGLVDGFHIDMKLPYHLLNEDDFDLVELTIGLRLDNLNLITRL
                     LQAIIYAVESDRGYSKVRSVRYPFLSDTAFEENRAYIASLNAKFYKAVPYEVNDFIYE
                     EE"
     gene            complement(12258..14201)
                     /gene="nrdD"
                     /locus_tag="PLH17_03135"
     CDS             complement(12258..14201)
                     /gene="nrdD"
                     /locus_tag="PLH17_03135"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HOR20799.1"
                     /translation="MGMFKTTNELIDKFIGNDDDKRADYLKRENSNFVYSLPLMRKNL
                     SNRAEEEYLMTNILPKKLVDLYEDGVVYIHDKQLSSYCQSISCRDVAMRGIPSLAKNM
                     LESDAASSLFTLIRHFSNIVTLMSQQVSGAVMLAQMTTILASYLYDEELRGVDYSDKL
                     LVRLMYQLIYELNLPLRAGSESAFSNCTLEFGKPSEDIKDEYVVIGGEAREYKYSEIP
                     ATYFDRVNVAFISAMAKGTNTGIPLTFPLITVQIDDNFNYHNPVFLLLLEKMYAWGGV
                     YFENFRTEAFNDPYYKNLNPLIKAKDPAVSRSLCCRLNINLGLLSRLGGGIFGSSVGN
                     VGAIQVLNLNMNRVLIEFGHDEQLLKEKIREYMEIMEEGHLAKRQWVEDHKELYPTFF
                     SFNKNLKNYFNVFAVTGMHEGLINVGYPLGMNDPQGKMYAHELMQYMSSIVDDFIVRD
                     KVACGIEYAPAENAGIKLARNDIKWAERNGRKIFVQGEADNVFLTSGCMLPFSEPDFT
                     KQIENAAEFQGYATSGSILHHFIESKIEPDILAKYIERLFRKPINYITLSPTVSSCLS
                     CGQTLAATDALNITECPVCHSNDIGTYSRVIGYVRMIARKNIKINEKGYYRGDYNFWS
                     KARRFDWAQRRRFHESDTDDQVL"
BASE COUNT         3836 a         3227 c         3413 g         3963 t
ORIGIN      
        1 tctttcggga ccgtgatggt cagcacgccg ttttccattt tggcggcgat atccttttct
       61 tcgacaccgt caccgacata gaagctgcgg cactccgact gatagtagcg ctcgcgatgg
      121 atgtagttct ccttttcatc attggtctcg cctttttgtg cggccttgat tgacaagtag
      181 ccattttcaa gcgagatctc gacgttttcc ttggccaacc ccggcagatc aaccatcaat
      241 tcataatggt tgtctttggc cacgatatca gttttcatcg tggtgtggcg gcggatatcc
      301 ggccaatccg ttttgaaaag atcaagcggg aaaaaatcgt cgaaaaatgg atcgtgaaga
      361 actagttctc cttttctatt tctcatattt tcacctccct tttcagtgtc ccccttaagg
      421 ggcacttcta ttgtagcata aattttagca ctgtcaatag gagagtgcta acgatatgct
      481 cagaggttta atatgtttta ttcgacggtg acggattttg ctaagttgcg tggcttatcg
      541 atattcagtc ctcgcttcaa agcgacataa taggcgaggt actgaccgaa gagcactttg
      601 acgagagaac ttaagtattt tggggcataa ggaacaacaa agtcgtcttc cggatgagcg
      661 agggttatgg aactgacgac gatgacgcga gccccgcggg cttctacttc atgcaagttg
      721 gagcgcatcg cgagctcggt tttggcatcg gagataaagc cgatgaccgg cgtatctttt
      781 tcaattaagg caatcggccc gtgttttaat tctccgcccg gaaaagcttc gctgtggata
      841 taagttattt ctttgagttt gagcgagcac tctaaggcca aatcataatc aaggccgcga
      901 ccgagaaaat agacatcgtt cttgcccgat acaaaggagg cgaggttttt gattttgtcg
      961 tggttatcga taacgctttg ctgagcggct atcaccgctt ctaaatcggc gatgaccgat
     1021 gcgtcatcat ttagcgcgcc cgccagcaaa gctaaggcgc tgacttgggc gacataagct
     1081 ttcgtggatg caacggccac ctcgatgccg gcgtaaagaa gaagggtgtg gtcggcgctt
     1141 cgagaaagcg tcgaaccctg agtgttggtg atggtaatga ttgtaccatc cattttttta
     1201 gcgacagaaa gacagtgaat taagtcggcg gtttcgcctg actgactcat aaaaatatag
     1261 accgtttttt tacctgatgt ataggggtta taagcccatt cgctggcgat atagacatcg
     1321 cttatttttc cggcttttct aaagaaacga ctgccgagaa ggcaagcgtg ataactcgtg
     1381 ccgcaagcaa gaaagacgat attatcggcc tctttaattt gcctcatcat ttctttatcg
     1441 aacaaaaatg atttgccgtc gtaataattg tccacaagaa gtttgacaca gctatcggct
     1501 tcttcgattt ctttgagcat ataatgggga taaccattta aatcaagagc ttcgggtttt
     1561 aactccgtct catgaaagac gataggaaga ggatgatcgt ttcgaccaaa cattttgatg
     1621 tcgtcaagcg aaatcgaacc atattggtag tcgctgatgg tgacgaactg atcggcgacc
     1681 ttaagcatcg gaagataatc ggaggcgagg aagtttccgt tctgtccgcg accgatcaga
     1741 agcggagaac ggtttttcgc aaaatagatg atgtcgggct ctttgttaaa caggatggcg
     1801 agggcatagc ttcctttaac atcgctcatc attttgctga tggctttata tacatctcct
     1861 gtgtggcggt agttttcttc gatgacattg gcgatgactt cggtatccgt ctcgcttaga
     1921 aatgaataac ctttttccat cagggcgttt ttaacagcgc ggaaattctc aatcacaccg
     1981 ttgtgaacga tgctaaaaag tccggacttg ctagcgtgag gatgagcgtt aatgaccgag
     2041 ggctcgccat gggtggccca ccgcgtatgc ccgatgccga ccaatccatc aatccttttt
     2101 tctaatttgt tatctaagtc ttcaactcga cctctaacct tgtaaatctc ggcttttttt
     2161 ccgttaaaca cggcgattcc ggctgaatca taaccgcgat attcaattgt cttaagtcct
     2221 tcgatgagat aattgcgaat cggtttattc gatacgcctc cgacgatgcc acacatgatt
     2281 tctttcctcc tgctgattaa ttatacaatt aaatcggaaa gcgttggata aggatagtat
     2341 tccttgggga gaatccgctc ggcctgattg cataaatctt tgatttcttg aagcaaaggc
     2401 cggatgctat gaacgcacat ttcggctttt tctttcttgc tgtgatttct ggaggcgagt
     2461 ttaatgccgg cggatagcag ttcattatgg cggtcgagtt catcgataag gcggccgcat
     2521 tcggcgctcc gcttggcgat agccgtcgcc gaaccgttca tcgcaatcgc cgtgctctta
     2581 tagagggcgg cggttttaat caatgccgga ataatgccct tgtcgaccat cgacacgagc
     2641 gtttttccct gcgtgaggac ggtttcaata aattcggtat agtaaatttc atagcgggca
     2701 tcgatttccg cttcgcttag gacatttaaa gaagaaagca tgcggcggac gagtggcgtt
     2761 ttgagcgtct cgacactgtc gatgtaatgc ggatagtttt cgagaccgcg acgagctgct
     2821 tcctccgccc attctttttt atagccgtca cccgcaaata atacgcgatg atggcgagaa
     2881 aggatttcgt gggcgagttt aataattccc tcgctgagtt tctcgggttc gatatctttt
     2941 agtttgaggt cgatttcacc aagagcctcg gcgatgccgg ctagcaaaac ggtgttgagg
     3001 gcggcgggat tgcgcgatga acctaaaatc cgaatttcaa acttattgcc ggtaaaggaa
     3061 accggtgaag tccggttgcg gtcagttata tcgagcggaa gagctgagag gttattgatt
     3121 ggcgaatata acttttcaaa ttccagcggc gtcacattcg ggcgattggc caattcctca
     3181 aacacgcggt gaaggtggtc gcccaagaag acggaaacga tcgccggagg ggcttcgtga
     3241 ccaccgaggc gatagtcgtt tccggcatcc gagcacgcca tgcggattaa tgtcgcatag
     3301 cggtccaccg cctgaataaa ggcggttgcg aaaacgagga atctgagatt tgaagagcgg
     3361 tcttcgccga gttcaaaaag attcatgccg gtgtcggtca tcaacgagaa attgttgtgt
     3421 ttgcccgacc cgttgacgcc gtcaaacggc ttttcattta ataagcagat taagtcgtgg
     3481 cgtaaggcca ttctttttaa gatgtccatc acgaccatgt tttggtcgac cgccacattg
     3541 acgttgttat aaatactgac aaactcatat tgtccgggag caacttcatt gtgctcgatc
     3601 gaggcgtaaa tgccgacttc ccagcacttc tgattgactt ccttcataaa tgaagagacg
     3661 cgttcggtca aagcgccgaa atagtgatca ttcatgcctt ggtctttggg catgctctca
     3721 ccaaatagcg ttttcccggt aaagacgata tccggacgca ttaaagcctg ggccttatcg
     3781 accaagaaat attcttgctc aagaccgatc atggctttga cggatttaaa accatcatag
     3841 cctagtttcc gcaaaatctt tgatgcgttc acattcattt tttctaaggc gacgagcagg
     3901 ggaagcttct gatcgagctt cgcgccggta tagctgacaa acacgcacgg tataaaaagg
     3961 acatggcctc tgacaaaagc ataagaggta atatccaaat aagtgtagcc gcgcgcttca
     4021 aaagccgtgc ggagtccgcc gttaggaaag gacgaaccat ccgtctcacc ttttaaaagc
     4081 gactttcccg atagacgggc gatcggctcc ccttcgccat ccatctgaat gaacgattcg
     4141 tgtttttcgg cggttaatcc cgtcatcggc tgaaaccagt gacagaagtg ggtggcgcct
     4201 aaatcgatgg cccagatctt catggcgtgg gcgatggcgt ctgctgtttc ccggtcgatt
     4261 tcggtctcgt ggcaaagggc ctgttgatac tttttaaaga ctgataacgg cagcgagctg
     4321 cgcattactt tctcattgaa ttgctttttg gcgaattctt cgatgacaac ttgtttttcc
     4381 attgttaata ctcccatttt ttacaaaata aaaagattgg gataattgct cataattata
     4441 aaagcaagca tccatgcaaa atcgcacttc agtacttcat atttaaattg aatccactat
     4501 ctatatatcg aaaatgttcg atgtatttta agatgaaagg ctaagcatga tcagggctct
     4561 gtgattttgt cccttttcta acggccgctc gggccgacaa ggcacccgcc gaatctttcg
     4621 aaaaccttgt tcctcgtcac cctctttaaa tagagggcca ggcgctatct atagtgaata
     4681 atggattaac ctccttttcg ttccatcttg cctttattat acatattgaa aaccatagca
     4741 caaaaatttt ttgctctccg agagatgaaa gcgctataaa aaatgctgaa aaatgcgaat
     4801 tatagtaaaa taagtgacat gaaagaatct cttctctatg acaagttgcc taaaggcaaa
     4861 gtccgatgca atgtctgcgg ccaccactgc ctgatcgttc ccggctatcg cggcatttgt
     4921 cgcgtcaggt caaacatcaa cggtgttttg tattcgctta attacggcct ttgcatcgcc
     4981 gccgccatcg atccgataga gaaaaagcca atctatcatt tctggccgag aaccgaaaca
     5041 tactcctttg ccgccgtcgg ctgcaacatg cattgtccgt ggtgtcaaaa ttacgaaatc
     5101 tcgcagacac ccagtaatga atatgtcgtc gagggcgatg aagtgacacc cggtgagcat
     5161 gtcgcgagag ctttgaagtt tatgacacct tcgatttctt atacctacag tgagccaacc
     5221 gtcttttttg agtacgctct tgaaacgatg aaactcgctc gggaaaaagg tcttaaaaac
     5281 atctgggtca gcaatggttt tatgagtgaa gagatgcttc gcgaaatcct tccctatctc
     5341 gatgcggcaa atattgacat caaaggtccg gatgatgaat tctacgccaa gcactgcggc
     5401 ggcaatctga aaacaattat caataattta aaaacaatgc aaaaggccaa cattcatatc
     5461 gaattgacga cgctatttat ccccggacta aatgacgagg aatatcagat taagaaattg
     5521 gtggcggcca tcgtcgaaaa tctcggtctt gaagtgccgt ggcacatcag ccgctttttc
     5581 ccggcctggc gcatgcttaa tgcccatgtc acgcctcttg aaacactcca tctcgcacgc
     5641 tccatcgctc aaaaagccgg actcaaatat atttacatcg gaaatgtcta gcatgaataa
     5701 tctccgcgca tattttgttc cccatccccc gctcattatt cccgaaatcg ggagaggaga
     5761 tgaacaggct atcaaagcca ccattgcggc ttaccagcaa gtcgctaaag aaattgcggc
     5821 atataaaccg gatacgattg tgattatctc gcctcacgct ttgacttatg aagattattt
     5881 tcatatcgaa ggcggtttat cctcgcgagg taatttcgct gactacaaag cgcctcagat
     5941 atcgtttaat gtcgcgcatg atgtcgatta cgtcaagaag ctggcctcct ttgcgaagaa
     6001 aacgactttt ccggccggaa ctgacgggag ggcgaataat ccgcttgacc acgcgacgat
     6061 gattccctta tatttcatca atcagcatta ccgcgaatat caagtcgtgc gactctcttt
     6121 ttccggcctt tcttttttaa ctcactacaa ctacggaaag atgattaaag agataattag
     6181 gagcgatgcc acaaaaaagt atgccgtcat cgccagcggc gatttatcgc atatgctgaa
     6241 aagcgatggt ccatatggtt atgttgaaga aggacctctt tttgataaag ctgtttgcaa
     6301 agtgctcaaa agtggtaact tcctcgattt gtttaatctt gatccgcaga tggttgaagg
     6361 ggcgggcgag tgcggctttc gctcgctatt aatcctcgcc ggcactttag acaaattggc
     6421 ggtctctgca aagctcctct cctatgaagg gcctttcggt gtcggctatg ccgtcgcttc
     6481 ctttgaagtc atcaactctg acgagaagag aaattacggg gctcagcacg aagaaaaaga
     6541 ggagaagaag ttaaaactca tccgccgcaa agaagatgaa tacgtctcac tggcccgcca
     6601 aacgcttgaa ttatatgtaa aaaccggcca agttttatct attgatgaag catctgatct
     6661 gttaaagcga actcgcgccg gagtgtttgt cagcttaaaa aaacacggcg tgctgcgcgg
     6721 ctgtatcggt acgattcagc caaccacgaa atcaattgcc catgaaatta ttcaaaacgc
     6781 catttcgtcg ggaactgaag atccgcgctt tccgccggtg agcatctccg agctttctga
     6841 cctcgtctac agcgtcgaca tcctgaaaga gcctgagccg atatcatcaa aaaatgaatt
     6901 ggatatcaaa aaatatggcg tcatcgttcg ctgtcgcggg cgaagcggtt tgcttcttcc
     6961 caatatcgag ggtgtccatt cagtcgagga acaaatcagc atcgctttga gtaaagccgg
     7021 tatttctaag cacgaactat acactctgga gcgctttgaa gtcgttcgtc atcagtgata
     7081 aaaaataaga aaaacactga cggtgacatc agtgtttttt gtagttgata atggctattt
     7141 cttgccttcg cgcttgaagc acataaacca atcgaggcaa tacttatcag gtgaggtatc
     7201 ttccatgcgc tctttagcgg ttccgcacga gccggcagtc gggatgataa catcatcacc
     7261 tggacgccag tcggcaggag tcgcgacttt atcagtatcg gctttttgca aagcctgaat
     7321 gatgcgtttg atatcatcaa agttgcggcc catcgaaagc ggatagtata aaatagtgcg
     7381 aacgatgccg tttggatcga cgacgaagac ggcgcggacc gcctgcgtgt tggattgtcc
     7441 gggttgaatc atgccatact ttttggcgac atccatgcgg atatcttcga ttaacggaaa
     7501 tttgacttcg atgtctttga agtttttcca ttcgatttct ttgatttttc tgagccaggc
     7561 aatgtgggcg tagagcgagt cgacggataa tccgacaagc tcggtgttga gggccttaaa
     7621 atcatctgct aatgaggcaa aagtcataaa ttcagtcgtg cacaccgggg taaagtccgc
     7681 ggggtgcgag aagaggatga cccatttccc cttgtagtct tccgggaaat taatcattcc
     7741 ctgagtggtc actgcggtaa aggcaggagc cttgtcgcca atcaacggca ttctgtagac
     7801 gttttcttcc atgtttttct ccttatatta tgtaatcgcc atagtaaaaa actatgatgc
     7861 ttataacatt attttaacat cttgaatatg cgtttataag gcaagtgctc aggataaaaa
     7921 attaaactgc agaatggtgg ggaagaagat cattaaaata ccgatgacaa gcattaatat
     7981 gccaccgacc aagtggttgt agcgggcgat tttattggac acgactttaa tttttaaagt
     8041 cagcatcaca atcgtaaaga cgataatatc atcgatgacg aagaagaaga tatagagtaa
     8101 gacatagccg actccagacc atcccccgag atcattcaga gccaaaatat aagcgaaaat
     8161 aagggggact ccggccgagc aggccagttc gataaaattg acgatcaacg ctaacgagat
     8221 gacacctaga agggcgagaa tgagtttgtt ctgagtgatg attttacgaa cgcgatccat
     8281 cagttttgtc cgctgctttt catccgtcgt ttcgcagccc acatcctttt ggcgaatgtt
     8341 tttaataaac tggtaaagat tgtagcctcc ggccgccaga gcaaaagtgc cgacgatgat
     8401 gcggaaggcg aggttgccgg cgatgaaaac cgccgtatta atccacgcca tcatcaaagc
     8461 gaaatagaaa agagctgagg tcaagataaa gatgccgccg agaatgaaga tgcgcttacg
     8521 gtcattattg ggtagcaaaa gagaaattaa aaaaagtaaa acccacatcg cacacggatt
     8581 aaaaccgtcg acaaagccca aaacaacaga gacaagacca agcgagatgg ctttgggatc
     8641 aatcactccg agaaagggca gctcgacttc gagttctgtg ctatcaagat caccgggttc
     8701 aatcgacgcg acaccctgtt caattatttt tgagacgaca tcaacttgcg gcgtctgact
     8761 atatcgctca atactttgcg tgatgaagta gcggacggtc acatcataac cggaaaaagc
     8821 ttttcctccc agagcagtcg tcgggacgcc gacggaaaaa tcaaacactt cggcgatgtc
     8881 ttcaagaagt tgcatattga ccgcatagtc gggattcgcc ccgcccgttt taagatttgc
     8941 gtcgattaag taggagacga ttgtaacgtt atcgtattga ctgtcaccgg cgaggctgct
     9001 taagaagact ttttcctgac tgcagtaatt gcagctttcg ctataaaaga agtgaagctt
     9061 gacctcgagt ggcgcctccg cggctcgccg aacttcttgg ggggcgatat cgccaccgct
     9121 tgccgataaa acgaaaacag cagcggccgc caagaggagt ttctgccact tatttgccat
     9181 tgagtaacgc ctcaattttt aacgtcattt ctttaagcga tttttcgccg atgaagcggg
     9241 cgacttcaat tccttcttga tataaaatca agaccggcaa agtcttccat actttatgct
     9301 ccgccaccgc ctctttatcg gcgtcgaaat catattcgag aaactcgatg tccgggtact
     9361 tttcgcggag ttcgtggtag cgcccgatca tgatgaggca cgacgggcac cagacggcgg
     9421 agatgatgac ggctttcatc ggctatttcc cgcctttttt aatgccgaaa cgaatgttct
     9481 tatttcctgt ttggtggtcg acggcgaaag gctgacgcgg aggctcgaaa gggcccgctt
     9541 ttcatcgccc gtcaaagcta atacggcgag tgattttctg gtattgctcg aacaggcact
     9601 atggttagaa atatatatct cctgtcgaga aagaatttcg actactcggc ggcttgaatg
     9661 gccaagcaag ctgaaattca agatatgcgg caaagcctcg gagggactat ttatgacgac
     9721 agtgggaatt ttcgttaaaa gttcacgaag atatgtattc aaggacttga ctttagcata
     9781 cttttgctcg tgttttttat agacttgaaa caaggctttc cataaggaaa gaataagagg
     9841 cgtattcgga gtgccggccc gatagatcgt cgtgctcgaa ccgccgtgaa gctgtgcgtc
     9901 tagtgcgacg cctctccttt taatcagagc gccgatgcct ttaattccgt aaaacttatg
     9961 agctgaaaaa gtgacgagat cggcaacgtg aaaggggacg atttccttac cgatgctctg
    10021 cgttgcatcg caatggaaaa gagcgcctga ggcatgggca atttttgcta tttcttcgat
    10081 tggctggcga atgccgattt cggaattaac cgatccgatt gagacgaggc cgacatcggg
    10141 attcatcaac tcttggaggt gagttaaatc aacttggcca tggctatcga tacgaacgat
    10201 gctgacaaga aggccttttc tttgtagggc gttgattgga gcgatgattg aggagtgttc
    10261 aaagcttgtc gttataactc gatttttagt acccttcaag gcttcaatgg cacctttgat
    10321 ggctaagttg ttggcttcgg tcgcacagct cgtgtatatg atttcgtggt cgttaaagcc
    10381 gagcgtattc aatatttgtc gccctgcttt tttaatcgcg cttttgctca tacgtccgag
    10441 tttgtatcgg gagttcgcat tagcaaaata cttgtcgctg acgacattga aatatttcaa
    10501 gacgcgggag tcagttttag tcgtcgcgct ataatcaaga taaatcaccg ttaattctcc
    10561 aaatgtattt ttaattataa cacaaacaat aagtatcttt ttcaaataaa gagaccccta
    10621 tttaaggagt ctctacaccg gtattaacta taatttttcg aggatttcag gaactttatc
    10681 gagccaagag cctttaaaca attcgatcaa tcccaaatcc gagaggctcg aaagatgggg
    10741 atcgatcggg attttggcga ggactttaag attatattca gaagcgatat cttcgatatg
    10801 gctcgcgccg aaaatttggt aatccttgcc gttatcggga cagcggaagt aagacatgtt
    10861 ttcgacgatg ccgatgatcg gtatattcat catctgcgcc atcttgaccg ccttgctgac
    10921 gattaacgag gccagctctt ggggagaagt gacgaccacg atgccatcaa ccggcaagga
    10981 ctgaaacgtc gtcagggcga catcgccggt tcccgggggc atgtcgataa acatgaagtc
    11041 gatgtctttc cagacgacat ccgaccaaaa ggttttgacc atgttgccga tgatcgggcc
    11101 acgccagatg acgggatcgg tcacattatc gagcagtaaa ttaatcgaga tgacatcgat
    11161 gccggtttta ctcgccagcg gaatcactcc taaatcgttg gcgtaggccc tttctttcaa
    11221 accaaacaat tttggaatcg acgggccggt tatatcggcg tcgagaatgg cgacattgtg
    11281 atctaaacgc ttcatcaaca ccgccagcat cgcggtgacg atcgattttc cgacgccgcc
    11341 tttgccgctg atgacgccga tgactttctt aacactgctg tgctcgttta acttcgctga
    11401 aaaatcgact ttctcgtctg ttctttcatc gcaagtttcg ccgcaggtac tgcagtcgtg
    11461 agtgcaatct ttttccataa ttttctcctg aaataagcgc ttcaattata cactatgaat
    11521 ctttaaatat aatcaaagat gattatttac cagtcacaat cttccgtaaa atatcaaaaa
    11581 aaccagcaaa aagcggtttt ttgcttcgaa attattcttc ttcgtaaata aagtcgttta
    11641 cttcgtaagg aactgcttta taaaatttgg cattcagcga agcgatgtaa gcccggtttt
    11701 cttcaaaggc ggtatcgctt aaaaagggat atctgacgct tcggacctta ctatacccac
    11761 gatcgctttc aactgcatag attatcgcct ggagaagcct ggtaataaga ttgagattat
    11821 caagtcttaa tccgatggtt aattcaacaa gatcaaagtc atcttcgttt aagaggtgat
    11881 aaggcagttt catatcaatg tgaaagccgt cgactaaacc ttcttcaaaa agggttttca
    11941 tcttttcatg attcgttccg ttggtataga tgatgattgg cttatcacaa acttttcgga
    12001 cctgctttaa atcactccgt aagtcatcaa ttgcggcgtc taaatattcg ccaccgctga
    12061 agacgatata ttcatataag tctttttgtc taaccacgta ttttatgacg tcaccaatca
    12121 aataaaactc atcgtggctt ttttcgatta actctttaaa gttaaagcag tgaaaacagc
    12181 gaaaagaaca tcccgtcaag gcgtggacca gaagggcggg atgtcctttt atttcaccga
    12241 gagtaagcag cagattttta tagtacttga tcatcggtgt cactttcgtg aaagcgccga
    12301 cgctgcgccc agtcaaaacg ccgagctttg ctccagaagt tataatcgcc gcgataataa
    12361 cctttttcgt taattttaat attttttcta gcaatcatgc gcacatagcc gatgacgcgg
    12421 ctgtaagtgc cgatgtcgtt gctgtggcaa accgggcatt cggtgatgtt gagagcgtcg
    12481 gtggctgcca gtgtttgtcc acaggacaaa caactgctga cagtcggcga aagggtaata
    12541 taattaatcg gttttctaaa cagcctttcg atatatttgg caaggatgtc aggttcgatt
    12601 tttgattcga taaaatgatg gagaatcgat ccggaggtag catagccctg gaactcggcg
    12661 gcgttctcga tttgtttggt aaaatccggc tcgctgaagg gaagcataca acccgaggtg
    12721 agaaagacgt tatcggcttc gccctgaaca aagatcttgc ggccgtttct ctcggcccat
    12781 ttgatgtcgt tacgcgctaa tttgatgccg gcgttttcgg ccggggcata ttcgatgccg
    12841 caagcgactt tgtcgcggac gataaaatca tcgacgatgc tcgacatata ttgcattaat
    12901 tcatgagcgt acattttgcc ttgcggatca ttcatgccaa gcggataacc aacattaatc
    12961 aggccttcgt gcatgccggt gacggcaaag acattaaagt agtttttgag atttttatta
    13021 aaggagaaga aagtcggata aagttctttg tggtcttcaa cccactgccg cttagctaga
    13081 tgaccttctt ccatgatttc catgtattcg cgaatcttct ctttgaggag ttgctcgtcg
    13141 tggccgaatt caatcagcac gcggttcatg ttgagattca agacttgaat ggcgccgaca
    13201 ttgccgaccg aggatccgaa aataccgccg ccaaggcgtg aaagaaggcc gaggttaata
    13261 tttaaccggc agcagaggct gcgcgagaca gcggggtctt tggctttgat taaaggattg
    13321 agatttttat agtagggatc attaaatgct tcggtgcgga agttttcaaa atagacgccg
    13381 ccccaggcat acattttttc taggagcaaa aggaagacgg gattatgata attaaaatta
    13441 tcgtcgatct gcacggtgat taagggaaat gtcaaaggga tgccagtatt cgttccctta
    13501 gccatcgccg agatgaaggc gacattgacg cggtcaaaat aagtggccgg tatttctgag
    13561 tatttatact cgcgagcttc gcccccgatg acgacatatt catctttgat atcttctgag
    13621 ggcttgccga attcgagggt gcagtttgaa aaggcggatt cggaaccggc ccttaacggt
    13681 aaattgagtt cgtagattaa ttgatacatc aggcggacga gaagcttatc ggaataatcg
    13741 acgccgcgaa gctcttcgtc gtataggtaa gaagcgagaa tcgtcgtcat ctgggcgagc
    13801 atgacggcgc cggaaacctg ctggctcatc agggttacaa tgttgctgaa atggcgaatg
    13861 agggtgaaca aagacgaggc ggcatccgat tcgagcatgt tcttggccaa gcttggaatt
    13921 ccccgcatcg cgacatcgcg gcaggagatc gactggcagt atgaactgag ttgtttgtcg
    13981 tggatataga cgacaccgtc ttcataaaga tcaacgagtt ttttcgggag aatattcgtc
    14041 atcaaatatt cttcttcggc gcggtttgat aagtttttcc gcatcaaagg aagagaatag
    14101 acgaagttgc tattttctct ttttaaataa tcagcgcgtt tatcatcatc gtttccgata
    14161 aatttgtcga ttaattcatt cgtcgtttta aacattccca taatacaatc ctcccttatt
    14221 agtaattaca gtaatctgct ggtgatgggg gcgattccgc cgttttttgg cgcacaaaaa
    14281 aactatgttt tcattcgtct tttttgttat gagtcgtcct tatgtgtatg aagatttggc
    14341 gtcttcgtat tacatagtat aggcactttc aaaactctta acaaggcaat tttgaactaa
    14401 aaaatggtaa acacaaaaat accgccgatg ctatcgaaa
//
LOCUS       DAOSSL010000022        14231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_4336_length_14231_cov_15.212895, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000022 DAOSSL010000000
VERSION     DAOSSL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_14231_cov_15.212895"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLH17_03140"
     CDS             complement(<1..344)
                     /locus_tag="PLH17_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20800.1"
                     /translation="MTKRNILIFFQNKTTLVFSMMAPVLILVIYILFMGDFQINTIRG
                     FIPEGQSVSDADIATVANAWMISGILGISSLTISLNSMFVAISDRERRIVDDFTASPV
                     KLVNLTLSYFISA"
     gene            complement(379..1323)
                     /locus_tag="PLH17_03145"
     CDS             complement(379..1323)
                     /locus_tag="PLH17_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20801.1"
                     /translation="MEKDNIIEVRGLVKNYKKVAAVRGIDFVVKRGEFFAFLGLNGAG
                     KSTTINILCSIIKKDAGEIYIDGLNTDKDADKIKNKIGIVFQKSVLDNELSVLQNLYS
                     RASLYPLSRKEAETKISKLIEILNLQSIIRRPYGKLSGGQRRKVDIARALLHNPQILF
                     LDEPTTGLDPNTRIMVWEALNDLKTKTDLTIFLTTHYMEEVVKADHVVIIDEGKISAS
                     GSPDELKNRYTSDILRIIASESEKINQILADDKLSYVYRGGAYEVVIEKPISALSIIQ
                     RHAPLFDDFEVLKGNMDHVFLNVTGKKMDTSHEKESSL"
     gene            complement(1310..2077)
                     /locus_tag="PLH17_03150"
     CDS             complement(1310..2077)
                     /locus_tag="PLH17_03150"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOR20802.1"
                     /translation="MIPKILHYIWLGGKPKPASVLKCFESWKKYCPDFQIIEWNETNF
                     AKGRHPLVDYAIEAHNWAFAADIIRVIVLYEYGGIYVDTDYEIIADLDQMLKYDCFMC
                     YESKFWVGTAILGSKAGHPVFEKLVHRYDQEHGITFYSNPFSVHAVSAILRYFYKIKM
                     DGKFSVTDNIALLPSEYFYPISYITFKETRTEKTLGVHHYAGSWHSKKQKRGFRFAAF
                     SRKVLGRHIYGLFEKLVANDFYYKIKKQLKKMNDGKR"
     gene            complement(2273..2349)
                     /locus_tag="PLH17_03155"
     tRNA            complement(2273..2349)
                     /locus_tag="PLH17_03155"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2313..2315),aa:Glu,seq:ttc)
     gene            complement(2357..2432)
                     /locus_tag="PLH17_03160"
     tRNA            complement(2357..2432)
                     /locus_tag="PLH17_03160"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2397..2399),aa:Asn,seq:gtt)
     gene            complement(2498..2728)
                     /locus_tag="PLH17_03165"
     CDS             complement(2498..2728)
                     /locus_tag="PLH17_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOR20803.1"
                     /translation="MAKVNNVLEKFKKKLNIEAVDESKTLKDYGLDSLDMVELILELE
                     DEYKIKFTDEEMTSLQTIGDLVNAIHKKCGTE"
     gene            complement(2843..2989)
                     /locus_tag="PLH17_03170"
     CDS             complement(2843..2989)
                     /locus_tag="PLH17_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20804.1"
                     /translation="MSEIKGQLLGILLVVLIFAGVSTALITAFDGATSHVADQILAPV
                     EEPL"
     gene            complement(3032..3346)
                     /locus_tag="PLH17_03175"
     CDS             complement(3032..3346)
                     /locus_tag="PLH17_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20805.1"
                     /translation="MGSNIGTILSFLFVINVFLFGSDLINVQLLYSNLDAVAMTAGHL
                     IVERGGITAEVAEFVEAEANATIYCVNNCTPRFGDTYIYVVTTSYEPITMSDEPIEIS
                     IR"
     gene            complement(3336..4004)
                     /locus_tag="PLH17_03180"
     CDS             complement(3336..4004)
                     /locus_tag="PLH17_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20806.1"
                     /translation="MKPFEKAISAANLDYKKEVRKQLVILLTASLLLAAMIAIMRKPL
                     LFLFIPLFLLIYAVYMQMRYANLKSQFLFNLQKEFVKLFTYYQIYIRNGLNIYNALEA
                     LVSFASPTIKDYLETLLAQIDEDKTVMPFINFGKRFHSLAIEQAMISIYQMVDQGNDE
                     MRLIQFQNVFGRIADQHYQDEVNKIRKGLESLNALPLVGAGLITMMITFGIITMIGDI
                     IGGI"
     gene            complement(3991..4701)
                     /locus_tag="PLH17_03185"
     CDS             complement(3991..4701)
                     /locus_tag="PLH17_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20807.1"
                     /translation="MRIVIATVFISSLLGALGYIASGNLYIAGIIAVIYLIYGILYVK
                     RKIQRHGKMMNSAHECQQFINNYLLSMSIRNSHSDAFLNATINAGGAFKEELKHIEHL
                     AVREQIDYLNKYFRFDVYYMFLNILTLFEEQGGDILTMAETLIQEVNRMEEAMIGVSA
                     IINRKIVEFVVLWTITLGIIIFMRFGMGQFYQQLLNAALVITMTSSLFILLLISLHLA
                     INKFMNSPILEGNHHETI"
     gene            complement(4689..5762)
                     /locus_tag="PLH17_03190"
     CDS             complement(4689..5762)
                     /locus_tag="PLH17_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOR20808.1"
                     /translation="MNNENNERLIDFLEQSFLAPLIKEAAITDISYNGRSIYYQHNDY
                     GRGKSEIVIKSQEVHDFLRQIANLGEKQFSYSEPLLDMSFGRYRLNAVHYALGRVNDC
                     KTPTFSLRIGSYETRIAADDSFMPMLAGEILDILIRNHQSLVIGGITGTGKTELQKYL
                     LSRIGKHERVVVIDNIQELTYSSANENIDLTCWQVNPYLQHASFQELIRNALRSNPDW
                     LVIAESRGNEMLDVLNSVMTGHPVLTTIHARSAETIINRMVRMVLMNGRETNYLEAES
                     DIKEHFRYFIYLRKETSADGRITRFLSSFLEYDHVTKTSVLIYQKEGDAIKYQLPSAH
                     TLQLIRSSPNGERIMENLHRCAS"
     gene            complement(5755..6144)
                     /locus_tag="PLH17_03195"
     CDS             complement(5755..6144)
                     /locus_tag="PLH17_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20809.1"
                     /translation="MINIWIGVISMSILLTGFTFSLKISAINRTFLLLPSGIIEKAVT
                     IIGLNEGEKPFYDTSYLENAVKDYFVDNLQSFVPSFSLAYYYFNQNEEGSYLLDRYDG
                     VVIGLRVPLILDNHYQNSLTFIIKNHE"
     gene            complement(6141..7127)
                     /locus_tag="PLH17_03200"
     CDS             complement(6141..7127)
                     /locus_tag="PLH17_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20810.1"
                     /translation="MPLLLLCTSFQSEQKLGWISFIVQPLSLESDQEIRFLYRALATS
                     DNQQFLLRIYLRNDKYPNRILVFQNSIFVSKGDTQDISRLFVLDHTLVDNSMNELTFS
                     CVSNGIEEQVIVKAALRFPSPFYPSEEQVSLTTPYNYYELRDGVLSRKRDMFVFNGFS
                     SIVDDDYYYYLDLSGLSIFSSNAFTYSKVSLLIEESENLYRLFPESVPSGFRKLPVQI
                     VARDLNNYDIRLAENLYVDPDTLLMSPKMREGFIATSKFYFPKDYYDTEHDVRFKLLI
                     DGCGYNEIDIIYDFYYYSHLKLIGDCRDSQYCIGVTSTEDEGVLTDWEETRP"
     gene            complement(7171..7518)
                     /locus_tag="PLH17_03205"
     CDS             complement(7171..7518)
                     /locus_tag="PLH17_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20811.1"
                     /translation="MGKCNNRRKFRNLCNDVREVARLYFKALQSANVRSGKYDLNMAV
                     YSQAYQGEDVHKLYISKIENAFRLLDVDSKRIINNDFFFNNYKFWWLSLYSTATYYRL
                     KRVAMCHFLENLA"
     gene            7845..8390
                     /locus_tag="PLH17_03210"
     CDS             7845..8390
                     /locus_tag="PLH17_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR20812.1"
                     /translation="MIILVGASASGKTEIAKILARKYGIAKAITHTTRPMRDGEEDGR
                     DYFFVSKEDFQKLLAENFFVEMTLYNENYYGCSKSQVSDNRAVIVDPNGLKAFQKLND
                     ASVISFLVVADEEVRYQRMLTRGDKKENAIKRINNDRVDFSSENVGNTNYVIVNQENS
                     LEEAADSIYQKYIAALKRIKN"
     gene            complement(8387..9964)
                     /locus_tag="PLH17_03215"
     CDS             complement(8387..9964)
                     /locus_tag="PLH17_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR20813.1"
                     /translation="MLLVDNVTLQFGGRVLFKNVHLTFDKGNCYGIIGANGTGKSTFL
                     QILTGELEPNKGEVVVTDKERISSLKQDHHAYDEFRVVDAVLEGHKRLVQVQRLRDAL
                     YAKPDFSEEDGLKAAELEMEYAELNGYEAESEARTLLNSLGILTEAQDRLVGDLDQRQ
                     IVKVLLAQALFGNPDILILDEPTNDLDIQATRWLENFLYNFENIVIIVSHDRHFLNKV
                     CTRILDVDYGKITSFVGNYDFWYQSSQLILEQSKRENVKKETRMKELETFIARFSANA
                     SKSKQATSRKKELEKITLDDLKPSSRRYPFVEWKFEKEVGNNVLEVKNLAQAGLFSDL
                     SFTINKGDKVAFIADNSQILTTLFDILGGLSEADGGEYRYGVTITPTYLPSDNTPFFA
                     GHDENLVDWIRPYSKDQSETVLRGWLGRMLFSGEEALKPVNVLSGGERVRLMLARMML
                     VAGNLLLLDDPTNHLDLEAITALNKGMVNYRGVILFTSHDHELIQTVANRIIYIKEKI
                     VVDKYLSYDEFIEQYNI"
     gene            complement(9967..10893)
                     /locus_tag="PLH17_03220"
     CDS             complement(9967..10893)
                     /locus_tag="PLH17_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOR20814.1"
                     /translation="MGIIKYQKYLDSHIGDLSNKTVVITGANSGIGFYAARLLAFKGA
                     RIIMACRNLGRAEEARKAIIEAIPHARIEILIYDQASLASIEQFADELTAKYPLINGF
                     VFNAGIFHPKKDAVTKDGYPLTIGTNYLGTFYLSELLRPYFAKMEDINVVCVGSLANR
                     RYKYQEITTYFTSKKTTLYRQYALSKKFMMANHYFFMKQKDTNIHYSMMHPGVASTNI
                     VSGTSNSFPRWFSRIARGFMSAFMNHPEKAALGIAILAVQEKMHGCYLLPRGLFEISG
                     YPKSKIVPQKLANNAEIIHEKTLAFFRELGKL"
     gene            complement(10881..11060)
                     /locus_tag="PLH17_03225"
     CDS             complement(10881..11060)
                     /locus_tag="PLH17_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20815.1"
                     /translation="MLNFDKNNKMTPRPDSEPKFDPKNKLDRKIVTFALLGLLVVAVL
                     IAVLVVVIELDLWAL"
     gene            complement(11149..12417)
                     /locus_tag="PLH17_03230"
     CDS             complement(11149..12417)
                     /locus_tag="PLH17_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOR20816.1"
                     /translation="MMTPLEIIVLVAIVVMVAFSAFFSSADMVYSTVDTLRLKKDAEK
                     GSKSAALALKFANEYDKTITTILFSNNLVNIAASSLASISYFGDILGPSLITVIMFLI
                     IVTFAEIIPKVIGRVYSHGLAKLYAYPILIFKYLFIPFVYATSALGRLIVSPLYKKAE
                     DEEEAVSDDELQEMVDTIEEEGVIDEEQGELLRSAITFMDTQAYEIMTPRVDIFAFDI
                     EDDISELINDDNVFMYSRVPVYEETIDNIIGILPTKLLLKAMLSNEKIDVRSLLTPVT
                     FVPRSRNISAVLQDFKKTHNHIAIVKDEYGGTEGLITLEDIVEELVGDIWDEMDEIDE
                     EATELGDGVYQIDGAMNIEDFFELVELELDEDADYSTVSGWCVSILERFAKVGDRFTY
                     ENLDVKVIQADEFTVEKIRVKVLPRKDVDE"
     gene            complement(12417..13454)
                     /locus_tag="PLH17_03235"
     CDS             complement(12417..13454)
                     /locus_tag="PLH17_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOR20817.1"
                     /translation="MPDPRSWLYILLIILLLVASAFFSLTETAFAAMNQFRMKVKADE
                     GSKPAKLVVKIHEKYDRTLIATLIGNNIVAILASVISTVLFLMLLRIYGVSDEVISVI
                     ATVVTTLLFYLFGDTIPKLIAKALPDKAAMFSSYIIYGFSIILFPVIIIFQGVIWVVY
                     KVFRFKEKPTLTRDDFTNIVESIEEEGLIEENESDIIYASFDFVDTSVKEVLTPRAKM
                     FAIDLQGLTHEKLNQYLLETNYSRIPIYRGNINNIVGILHVKTYLHKYLKNRDIAIES
                     TLQKPYFVTTQIMMDEMIEGFKRSHTHIAIVRGSKDTVLGMVTMEDVLEELVGDINEF
                     SPKITKGKVKP"
     gene            complement(13550..14134)
                     /locus_tag="PLH17_03240"
     CDS             complement(13550..14134)
                     /locus_tag="PLH17_03240"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOR20818.1"
                     /translation="MDTINRPGWIEIVTGPMFAGKSEELIRRIKRLEYAKKKVLVFRP
                     RIDNRYSLDEVVSHSNNRRKSIVIDAAKDILPYISEDTYAVVIDEIQFLDHELISLSE
                     HLANIGIRVILGGLDSDFRGEPFAVTAEMMARAEFVTKLTAICVRCGSPATKTQRIVN
                     GKPAHYLDPIVVVGAAEAYEPRCRHCHEVLGKAK"
BASE COUNT         4073 a         3036 c         3177 g         3945 t
ORIGIN      
        1 gcgctgataa aatagctcag cgttaaattg acgagtttaa cgggcgaggc ggtgaagtcg
       61 tcgacgatgc gccgctcgcg atcgctgatc gccacaaaca tgctgtttaa tgagatggtc
      121 agcgaagaga tgccgagaat tccggaaatc atccaggcgt tggcgaccgt cgcgatatcg
      181 gcgtcgctga cgctttgacc ttcaggaata aaaccccgaa tcgtattgat ttgaaaatcg
      241 cccatgaaca agatatagat gactaaaatc agaacggggg ccatcatcga aaagacgagg
      301 gtcgttttat tctgaaagaa aatgagtata ttgcgtttgg tcaaatgcca aatgttatac
      361 aaataatcat caaagagtct aaagagacga ttctttttca tgacttgtat ccatcttctt
      421 tccggtaaca tttaaaaaaa catggtccat atttcctttt agcacttcaa aatcatcaaa
      481 aagaggagcg tgcctctgaa tgattgacag cgccgaaatc ggcttttcaa taaccacctc
      541 ataggcacca ccacgataga catatgacag tttgtcatcc gctaatatct ggttaatttt
      601 ttcgctttcg gatgcgatga tgcgaaggat gtcgctcgta tagcgatttt ttaattcatc
      661 ggggcttccg ctcgcgctta ttttcccttc gtcaataatg acgacatggt cggctttgac
      721 aacctcttcc atataatggg tcgttagaaa aatagttaaa tccgttttcg tttttaagtc
      781 gtttaaggcc tcccaaacca taatgcgagt gttggggtca aggccggtcg tcggctcatc
      841 taaaaagagg atctgggggt tgtgcaaaag agcgcgggca atatcgacct ttcgcctctg
      901 cccgcctgat aatttaccgt atggacgtct gatgatactt tgtaaattaa gaatttcgat
      961 caatttgctg atttttgtct cggcttcctt gcggctcagc ggataaaggc tggcccgcga
     1021 atagagattt tgtaaaacgc tcaattcgtt atcgagaacg cttttttgaa agacgatacc
     1081 gattttattt ttaattttat cggcatcttt atcggtgtta agaccatcga tgtaaatttc
     1141 gccagcatct tttttaatga tagaacacaa aatattgata gtagttgatt tgccggcgcc
     1201 attaaggccg agaaaagcaa aaaattcgcc gcgtttaacg acaaaatcaa tgccgcggac
     1261 cgctgcgact tttttataat ttttgacgag tcctctgact tcgataatat tatctttttc
     1321 catcattcat cttctttaat tgcttcttaa ttttataata aaaatcattt gcgaccaatt
     1381 tctcgaataa tccgtagata tggcggccga ggacctttct tgaaaaagcc gcgaaacgaa
     1441 aaccgcgctt ttgctttttg ctgtgccatg aaccggcata atgatggacg ccgagagtct
     1501 tctcagtccg cgtctcctta aatgtgatgt aactaatcgg atagaaatat tcgctcggga
     1561 gcagggcgat attgtcggta accgagaatt tgccatccat ttttatttta taaaaatacc
     1621 gaaggatagc gctgacggca tgcaccgaaa aagggttgct gtaaaaggtg attccgtgct
     1681 cctggtcata gcgatggaca agcttttcaa aaactgggtg cccggctttg cttcctaaga
     1741 tggcggttcc gacccagaac ttactttcgt agcacatgaa gcaatcgtat ttcaacatct
     1801 ggtcaagatc agcgataatt tcgtaatcag tatcaacgta tatccctccg tattcataga
     1861 ggacgatgac gcggatgata tcggcggcaa acgcccaatt atgagcctcg atggcataat
     1921 cgaccaaggg atggcgacct ttagcaaaat tcgtttcatt ccactcgatg atctgaaagt
     1981 cagggcagta ttttttccag ctttcgaagc attttaaaac gctcgccggt ttgggcttgc
     2041 cgcccagcca aatataatgg agtatttttg gtatcatcgc gcttgccctt cccccgagaa
     2101 acgaatccgc ccaaagcggt gttttattta ttatatcatc cgcgaaaaaa atgccgagag
     2161 cgagttgctg attttatgat aaacataaag gcgatgctgg aatggtttga attgaaggtg
     2221 aacacgaact acaaaataaa aaaacacaac ctgcattgtg taattttctt gttggtgacc
     2281 cgtacgggat tcgaacccat gaatgcatgc gtgaaaggca tgtgagttaa gccacttctc
     2341 caacgggcca cataagtggc gcctaccata ggactcgaac ctatgaccga tcggttaaca
     2401 gccgattgct ctaccgactg agctaggtag gcgtgttatt caaaagaata acgcaagaag
     2461 tatattacca aaacggcttt gaatatgcaa cgaagattta ttcagttccg cacttcttgt
     2521 gaatggcgtt taccagatca ccaatcgtct gcaaagaggt catctcttca tcggtgaatt
     2581 tgattttgta ctcatcttct aactctaaga tgagttcgac catatcaagc gaatctaaac
     2641 cgtagtcttt gagcgtcttg ctttcgtcaa cggcttcaat gttcagtttt ttcttaaatt
     2701 tttcgagaac gttatttact ttggccatga tttaatcctc actccgttgt attatagcaa
     2761 aaaataatct tttgtaagaa ggaaattaca tttaagaaaa agggcataac gcccttcgtg
     2821 atgagttatt gagaaatcga ttttataaag gctcttcgac gggagcgagg atttgatcgg
     2881 cgacgtgcga agtcgcgccg tcaaaagccg taatcaaggc cgtgctgact cccgcaaaaa
     2941 tcaagacgac gagcaagatt ccgagcaatt gtcctttaat ttccgacatg ggttctcctt
     3001 tcttttagtt gtaatatcct attacgacgc ttcagcgaat gcttatttct atcggttcgt
     3061 cactcatggt aatcggttca taagacgtcg taacaacata tatgtaagta tcgccgaagc
     3121 gcggtgtgca attattcaca caataaattg tggcattggc ctcggcttcg acaaattcag
     3181 cgacttcggc cgtgataccg cccctttcga caatgagatg tccagccgtc atcgccaccg
     3241 catcaagatt tgaatatagg agctgaacat ttattaaatc gctgccaaaa agaaagacat
     3301 taataacaaa aagaaaagac aggatagtgc cgatgttaga tcccaccgat aatgtctcca
     3361 atcatcgtga tgataccaaa agtgatcatc atcgtgatta agccggctcc gacaagcggc
     3421 agggcattca gcgattcaag gcctttgcga atcttattga cctcgtcttg ataatgctga
     3481 tccgctattc gtccgaagac attttgaaat tggatgaggc gcatctcatc atttccttgg
     3541 tcgaccattt gatagatgct aatcatcgct tgttcgatgg ccagtgagtg aaaacgtttg
     3601 ccgaagttaa taaagggcat gaccgttttg tcttcgtcga tctgagcgag cagagtttcg
     3661 aggtagtctt taatcgtcgg tgaggcaaaa ctgacgaggg cttcaagggc attatatatg
     3721 tttaaaccat tgcgaatgta tatctggtaa taagtgaaca gtttgacaaa ttccttctga
     3781 agattaaaaa gaaactgaga tttcaaattc gcgtagcgca tctgcatata aacggcatat
     3841 atcaagagaa acaggggaat aaacagaaag aggagcggtt ttcgcatgat agctatcatc
     3901 gcagcgagca atagcgatgc agtcagcaaa attaccagct gtttccggac ctcttttttg
     3961 tagtccagat tcgccgccga aatcgctttt tcaaatggtt tcatggtggt taccttcgag
     4021 gatgggcgaa ttcatgaatt tattgatggc aagatgaagg gagatgagaa ggaggataaa
     4081 taaggaggaa gtcatagtta tgactaacgc ggcattcaaa agttgctgat aaaactgtcc
     4141 cataccaaac cgcatgaaga tgatgatgcc gagggtgatg gtccacaaga cgacaaactc
     4201 aacaattttc cgatttataa tcgcggatac accgatcatg gcttcctcca ttcgattcac
     4261 ttcctgtatg agagtctcgg ccatcgttaa gatatcgcct ccctgctctt caaataatgt
     4321 caatatgttt aaaaacatat agtagacatc aaaacgaaaa tacttattga ggtagtcaat
     4381 ctgttcgcga accgctagat gttcaatatg tttaagttcc tctttaaagg ctccgcccgc
     4441 attaatcgtc gcgtttaaaa aagcatcgga atgagaattt cttatgctca tcgaaagcaa
     4501 atagttattt atgaattgct ggcactcatg ggcgctgttc atcattttgc catgtcgctg
     4561 aattttacgt ttaacatata agattccgta tatcaaataa ataacagcga ttattccggc
     4621 gatgtataaa ttgccgcttg caatatagcc aagcgcgcct aaaagcgatg agatgaacac
     4681 tgtagcaatc acgatgcgca tctatgaaga ttctccatga ttcgctcgcc gtttggcgat
     4741 gagcggatga gttgtaacgt atgggctgat ggtaattgat atttgattgc atcgccctcc
     4801 ttttgataaa tcaaaactga tgtttttgtc acgtgatcat attctaaaaa cgaagataga
     4861 aaacgagtta tgcgaccgtc tgcagatgtt tcttttcgca aatatataaa atagcgaaaa
     4921 tgctctttaa tatcgctttc ggcttcgaga taattcgtct ccctgccatt catcaggacc
     4981 attcgcacca tgcggtttat aatcgtctcg gcgctgcggg cgtgaattgt cgtcaggacc
     5041 ggatggccgg tcatcaccga attgagaaca tcgagcatct catttccgcg gctttcggcg
     5101 atgacgagcc aatcgggatt gctgcgaaga gcgtttcgaa tcagctcctg aaaggaagcg
     5161 tgttgaagat aggggttaac ctgccagcac gtcaaatcga tattctcgtt tgccgaactg
     5221 taagtgagtt cttgaatatt atcaatcacc accactcttt catgcttgcc gatgcgtgaa
     5281 agaagatatt tctgcagctc ggttttgccc gttcccgtaa tgcctccgat gacaagcgac
     5341 tggtggtttc taatcaagat gtcgagaatc tcgcccgcca gcatcggcat aaacgaatca
     5401 tcggcggcaa tacgggtttc atatgagcca atccgcaaag agaaagtggg ggttttacaa
     5461 tcattaactc gccccagagc atagtgaacg gcattgaggc ggtaacgacc gaagctcatg
     5521 tcgaggagag gctctgaata cgagaactgc ttttcgccta aattggcgat ttgccgcaaa
     5581 aaatcgtgta cttcctgact ctttatgacg atctcgcttt tgcctcttcc atagtcgttg
     5641 tgctgataat aaatgcttcg cccattataa gaaatatcgg tgatcgccgc ttctttaatt
     5701 aaaggtgcca aaaatgattg ttctaaaaaa tcgatgagtc gctcattatt ttcattattc
     5761 atggttttta attatgaaag tcagactatt ctgataatga ttatcgagta tcaaaggaac
     5821 gcggagaccg atgaccactc catcataacg atctaataga taactgccct cttcattttg
     5881 gttaaaataa tagtaggcca aggaaaaact cggcacaaag gattgcaaat tgtcgacaaa
     5941 ataatctttt acagcatttt ctagatagga tgtatcgtag aaaggctttt ctccttcgtt
     6001 aaggccgatg atggtgacag ctttttcaat aatgccgctt ggtagcagca gaaacgtgcg
     6061 attgatggcg cttatcttta atgaaaatgt gaaaccagtc aaaagtattg acatgctgat
     6121 cacgccaatc caaatgttga tcatggccga gtctcctccc agtcggtcaa aacaccttca
     6181 tcttcagtgg atgtgacgcc gatacaatat tgcgaatcgc gacaatcacc gattaatttg
     6241 agatgggaat aatagtaaaa atcatagata atatcgattt cattgtagcc acagccatcg
     6301 atgagaagtt tgaagcgaac atcgtgttca gtatcataat aatctttggg aaaatagaat
     6361 tttgatgtgg cgataaaacc ttctctcatc ttcggcgaca ttagcaaggt gtcaggatca
     6421 acatagagat tctcggcgag acgtatatca taattattaa gatctctggc gacgatttga
     6481 acgggcaact ttcgaaaccc cgaaggcacg ctctccggaa aaagacgata gagattttcg
     6541 ctttcttcaa tcaggagcga tacttttgaa taagtgaaag cattgctcga aaatatcgaa
     6601 agtcccgaaa ggtctaaata ataatagtaa tcatcatcga caatgcttga aaagccattg
     6661 aaaacaaaca tatcgcgctt tcgagataat actccgtcgc gaagctcata gtagttataa
     6721 ggggtggtta aactgacttg ttcttcgctc ggataaaaag ggctcggaaa gcgcagggcc
     6781 gctttgacga tgacttgctc ttcgattccg tttgaaacac acgaaaaagt aagttcgttc
     6841 atgctgttat cgacaagcgt gtgatccaag acaaagaggc gcgaaatgtc ctgagtatca
     6901 cctttactga caaagatgct gttttgaaaa acaaggatgc gattcggata tttgtcattt
     6961 ctaagataga ttcgtagcaa aaactgctgg ttatcagacg ttgcgagggc tcgataaaga
     7021 aaccttattt cttgatcgct ttcaagcgag aggggttgga cgataaatga gatccacccc
     7081 agtttttgct cgctttgaaa actagtgcaa agaagaagta aaggcatgag gaaaaagaga
     7141 gcacaaattc tacgaaattt ttgcatataa ttaagctaaa ttttctaaga agtgacacat
     7201 cgccacgcgt ttaagacgat agtatgtcgc ggtcgaataa aggctgagcc accaaaactt
     7261 gtaattattg aagaagaaat cgttgttaat aatacgcttc gaatcaacat ctaatagacg
     7321 aaaagcgttt tcaattttgc ttatgtaaag tttatgaaca tcttcgccct gataagcttg
     7381 cgaataaaca gccatgttca gatcgtattt gccacttcgc acattcgcgg attgaagcgc
     7441 tttaaaatat agtcttgcca cttcgcggac atcattacat aaattacgaa attttctccg
     7501 attattgcat tttcccattc ctaacctcct ttctcccctt tctagatttg taagtatcaa
     7561 agctgtcaaa atgtcaaaaa aatgtaaaaa gataagaaaa aattattttc atggaggaaa
     7621 ttgaaagcat ttaagatgtc taccattggc taaaactaag aaagaactgg tttccttagc
     7681 ctatgaattt taataatgag acttgcctcc atcgctgcaa caacggaaaa gtggttgctt
     7741 ttgttaacgc gactatttgt tatgattgta acataggaaa acagcgtgtc aaactcatta
     7801 aaagaccttt aataaataaa ggtaataatt tataatattg gcatatgatt attctcgtcg
     7861 gcgcctccgc cagcggcaaa acggaaatag caaaaatcct cgccaggaag tatggaatcg
     7921 ctaaagccat aacccacacc acacgcccga tgcgcgatgg cgaagaagac ggacgcgact
     7981 atttttttgt aagcaaagaa gactttcaaa aactgctcgc cgaaaatttc ttcgtcgaga
     8041 tgacgctata taatgaaaac tactatggat gctcaaaatc acaagtcagc gacaacagag
     8101 ccgtcatcgt cgatccaaac ggactgaaag cctttcaaaa actgaatgat gccagcgtca
     8161 tttcgtttct cgtcgtcgcc gatgaagaag tccgctatca gcgtatgctg actcgcggtg
     8221 acaaaaaaga aaatgccatc aagcgcatca ataatgaccg cgttgacttc agttccgaaa
     8281 acgtcggtaa caccaactat gtgattgtca atcaagagaa ttctcttgaa gaagccgccg
     8341 actcaatcta tcagaaatat atcgcagctc taaaaagaat taaaaattaa atattatatt
     8401 gctcgataaa ttcgtcgtat gaaagatact tgtcaacgac gattttttct ttgatataga
     8461 tgatgcggtt cgccaccgtc tgaattaact cgtggtcgtg ggatgtaaat aagatgaccc
     8521 cgcggtaatt gaccatcccc ttattaagtg cggtaatcgc ttcgagatca aggtggttcg
     8581 tcggatcgtc gaggagcaga agattgccgg caaccagcat catgcgggcg agcatcaagc
     8641 ggacgcgttc gccgcccgac aaaacattaa ccggtttaag tgcttcttcc ccggaaaaga
     8701 gcatgcgtcc gagccaaccg cgcaagactg tctcgctctg atcctttgaa tacgggcgaa
     8761 tccaatcgac gagattttca tcatgccccg caaaaaaggg tgtgttatcg ctgggaagat
     8821 acgtcggagt gattgtcaca ccataacgat attcaccgcc gtcagcttcg cttaagccgc
     8881 cgagaatatc gaaaagagtc gtcagaatct gactattatc ggcgatgaaa gccactttat
     8941 cgcctttatt aatggtaaaa gagaggtcgc taaataatcc ggcttgggca aggtttttga
     9001 cttctaggac attgtttcct acttcttttt cgaatttcca ttccacgaat ggataacgac
     9061 ggctcgaagg ttttagatca tcgagggtaa ttttttctaa ctcttttttg cgcgaggtcg
     9121 cctgcttgga tttcgaggca ttggccgaga agcgggcaat gaaagtttcg agttctttca
     9181 tccgcgtttc cttcttcaca ttttcgcgct ttgattgctc gaggatgagc tgacttgact
     9241 gataccagaa atcatagttg ccgacaaatg aagtaatctt gccgtaatcg acatcgagga
     9301 tacgcgtgca cactttattt aaaaagtgac gatcgtgaga gacgatgatc acgatgtttt
     9361 cgaagttata caaaaaattt tccaaccatc ttgtcgcctg aatatcaaga tcgtttgtcg
     9421 gttcatccaa aattaaaata tccggatttc caaaaagggc ctgggccaag aggactttga
     9481 cgatttgccg ctgatcgagg tcgccgacca gccgatcttg ggcctcagtc aaaatgccga
     9541 gggaattaag gagcgttcgc gcttcacttt cggcttcata gccgttgagt tcggcatatt
     9601 ccatctccag ttcggccgcc ttgaggccgt cttcttccga gaaatcgggt ttggcgtaaa
     9661 gagcatcgcg aagtctttgc acctggacaa gccgcttgtg accttccaat acggcatcga
     9721 caacgcgaaa ttcgtcatag gcatgatggt cttgctttaa cgatgaaatg cgctctttat
     9781 cggtgacgac gacttcgcct ttattcggtt ccagttctcc ggttaaaatc tgcaggaaag
     9841 tggatttgcc cgtaccgttg gcgccgatga tgccatagca gttgcctttg tcaaaagtca
     9901 ggtgaacgtt cttaaaaaga acgcgaccgc cgaattgcaa tgtgacatta tcaactagaa
     9961 gcatgtttat aactttccta attctcggaa aaaggccaat gttttctcat ggattatttc
    10021 cgcattattt gctaattttt gggggactat tttggatttc ggataaccgc taatttcaaa
    10081 tagtccgcgg ggcaatagat agcacccgtg catcttttct tgtacggcta atatcgcgat
    10141 tcccagagcc gctttttcgg gatgattcat aaaggcactc atgaagccgc gggcaatgcg
    10201 tgaaaaccaa cgcggaaagg aattggatgt gccgctgacg atattggtcg acgccacgcc
    10261 cggatgcatc atcgaataat ggatattagt gtctttttgc ttcatgaaaa aatagtgatt
    10321 tgccatcata aacttttttg aaagggcata ttggcggtaa agagttgttt tcttggatgt
    10381 aaagtatgtt gtaatctcct gatacttata tcgccggttg gcaagcgaac cgacgcagac
    10441 gacattaata tcttccatct tggcaaaata aggccgcagc aattcggaaa gataaaaggt
    10501 tccgaggtag tttgtgccga tcgtgagcgg gtatccatct tttgtaacag catccttttt
    10561 aggatgaaag atgccggcat taaagacaaa accgttaatc aatggatatt ttgctgtgag
    10621 ttcatcggcg aattgctcga tgctcgcgag tgaagcttga tcgtagatga gaatctcgat
    10681 tcgggcgtgc gggatagcct cgataattgc cttgcgggcc tcttcggcgc ggccaaggtt
    10741 acgacaggcc atgatgatgc gggcgccctt aaaagccagg aggcgggcgg cgtaaaagcc
    10801 gatgccgcta ttcgcgccgg tgatgacgac cgtcttattg cttagatcgc cgatatgaga
    10861 atcgagatac ttttgatatt ttataatgcc cataaatcta attcgatgac gacaaccaaa
    10921 acggcaatca ggacagcgac aaccaaaagg ccgagcaagg caaaagtcac gattttccga
    10981 tcgagtttgt tttttgggtc aaacttcggt tcggaatcgg gacgcggtgt cattttgttg
    11041 ttcttgtcaa aattgagcat tcgttttctc ctcatgtatt ttagcagtaa tcaagttttt
    11101 ttctattaaa aaaacgaaat ccgcaatcgc tagtcaaaaa agatgtcttt attcgtctac
    11161 gtctttgcgg ggcaaaactt taacgcgtat cttttcgacg gtgaattcgt cagcctgaat
    11221 gaccttgacg tcgagatttt cgtatgtaaa acgatcgccg actttggcaa atctttccaa
    11281 gatggaaaca caccagccgc tgacggttga ataatcggcg tcttcgtcaa gttctaattc
    11341 gactagttcg aagaaatcct caatattcat cgcgccatca atctggtaga cgccatcgcc
    11401 taattcggtc gcttcctcat cgatttcgtc catctcatcc caaatatcgc ccactaattc
    11461 ttcaacgata tcttccaggg tgataagacc ctcggtacca ccgtattcgt ctttgacgat
    11521 ggcaatatga ttgtgcgtct ttttaaaatc ttgtaagacc gctgaaatat tgcgcgaacg
    11581 gggcacaaaa gtgaccggtg tcaataaaga gcgcacatca attttctcat tgctaagcat
    11641 agctttgaga agtaatttag tcggcaaaat gccgatgata ttgtcgattg tctcttcata
    11701 aaccgggacg cgggagtaca tgaaaacatt gtcgtcattg atcagttcgc taatatcgtc
    11761 ttcgatgtca aaggcaaaaa tatcaacgcg tggcgtcatg atctcatagg cctgtgtatc
    11821 catgaaggtg atggcggagc gaagcagttc accctgctct tcatcgatga caccttcttc
    11881 ttcaatcgtg tcgaccatct cctggagttc atcatcactg acggcttcct cttcatcttc
    11941 ggctttttta tataaggggg agacgatgag acggccgagg gcgcttgtcg cgtagacaaa
    12001 aggtataaaa agatatttga aaatgagaat cggataagca tatagcttgg cgagtccgtg
    12061 cgaatagacg cgcccgatga ctttggggat gatttcagca aaggtgacga taatcaaaaa
    12121 catgataact gtgatgagac ttggcccgag aatatcgccg aagtatgaaa tactcgccag
    12181 cgaagaagcg gcgatattga ccaagttgtt ggaaaagaga atggtcgtga tggtcttatc
    12241 gtattcgttg gcgaatttta aagctaaggc cgcgcttttg ctgccttttt cggcatcttt
    12301 tttaagccgc agcgtatcga ccgtgctgta aaccatgtcg gccgagctga aaaaggccga
    12361 gaaggcgacc atgacgacga tggcgacgag gacgatgatt tcaagtggcg tcatcattat
    12421 ggcttaacct tgcctttcgt gattttcggg ctgaattcgt tgatgtcgcc gacaagttcc
    12481 tcaaggacgt cttccatcgt caccattcct aagacggtgt ctttggagcc gcggacgatg
    12541 gcgatgtgag tatggcttcg tttgaaacct tcaatcattt catccatcat aatttgcgtg
    12601 gtcacgaaat atggcttctg gagcgtcgat tcaatcgcga tatcgcgatt tttaagatat
    12661 ttatgaagat atgtcttaac gtggagaatg ccgacaatat tattaatatt gccgcgatag
    12721 atgggaatgc gcgagtagtt agtttcgagc aagtactggt ttaatttttc atgcgtcaga
    12781 ccctgaagat cgatggcaaa catttttgcc ctcggggtca agacttcctt aaccgaggtg
    12841 tcgacgaaat caaaagaagc gtagatgatg tcggattcat tctcttcaat caagccttct
    12901 tcttcaatcg attcgacgat attagtaaaa tcatcgcgcg ttaaagttgg tttttcctta
    12961 aagcgaaata ctttatagac gacccagatg acaccttgaa aaataatgat gacaggaaag
    13021 agaatgatcg aaaatccata gataatatag cttgaaaaca tcgccgcctt atccggaaga
    13081 gccttggcga tgagtttagg aatcgtgtcg ccaaaaagat aaaagagaag agtggtgaca
    13141 actgtggcga tgaccgatat gacttcgtca ctcacgccat agatgcgcaa taacataaga
    13201 aaaagcactg tcgagatgac agaagccaga atcgcaacga tgttatttcc aatcagagtc
    13261 gcaatcagcg tccgatcgta tttttcgtga attttaacga ctaatttcgc gggtttgctg
    13321 ccttcatcgg ctttgacctt catgcggaac tgattcatcg ccgcgaaggc cgtctccgtc
    13381 agcgagaaga atgctgaagc gacaagcaac aagataatta ataagatata gagccacgat
    13441 cgagggtcag gcatcgacaa ccaactcctt tattagacct caacgcgaaa gcaataatag
    13501 cataagtatt aaatgttcgc aaataaatta acgggcagac gcgcgtgggc tatttagcct
    13561 tgccgaggac ttcgtgacag tgtcggcagc gcggctcata agcctcggcc gctcccacga
    13621 caacgattgg atcaagataa tgagcgggct tgccgttgac gatacgctgt gtcttggtgg
    13681 ccggagagcc gcagcgcacg caaatcgccg ttagtttggt gacaaactcg gcccgggcca
    13741 tcatctcggc cgtcacggca aacggctcgc cgcgaaaatc gctgtccaaa ccgccgagaa
    13801 tgacgcgaat gccaatatta gcgagatgtt cgctcaatga aatcagttca tgatcaagaa
    13861 attgaatttc atcgatgaca actgcatatg tgtcttcgct tatataagga agaatgtcct
    13921 tagcggcatc gatgacgatt gattttcggc gattgttgct gtgactgacc acttcatcaa
    13981 gggaatagcg attgtcgatg cggggacgaa agaccagcac tttctttttg gcgtattcaa
    14041 gccgtttgat gcggcgtatt aattcttcgc ttttgccggc aaacatcggt ccggtcacga
    14101 tttcaatcca acccggtcta ttaattgtat ccatagtcga aacctctttt ctaaattatg
    14161 ccaaaaacct ccgggcatga caacgaaatc aacaaaagaa aaaggcgatt atataatcgc
    14221 ctttcgtgta t
//
LOCUS       DAOSSL010000023        12846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_4905_length_12846_cov_12.464311, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000023 DAOSSL010000000
VERSION     DAOSSL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_12846_cov_12.464311"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLH17_03245"
     CDS             <1..504
                     /locus_tag="PLH17_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20819.1"
                     /translation="YNVTDPRFTTYTSAVSSSMLLPQLYRFVDDVNPAKEGAYDYIST
                     FMNTTASECAALDVKSSTWSTLKSGYEILLDESKNYIYEHSENDVLIAAMVERYAVII
                     NKYGYDNFLTDALGNPVLMALNSDDGIDLLGSEAAIAAAIISFAGIIFISLAGFLFYQ
                     KTKKQIL"
     gene            567..1295
                     /locus_tag="PLH17_03250"
     CDS             567..1295
                     /locus_tag="PLH17_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20820.1"
                     /translation="MSKYLLGKISINFAVFICAMVGVIATSFGVSGLMTGVSILYYTV
                     QSNIWIGLTSLTFAILQIIGVKKSSVVITGWLYVTKYVFVVAITLTMVIFWLLLAPTI
                     QLPSYLLSPSNLFAHTLTPILSLASFSFFDSRDYKLSKKSSFFSLATPLYYFIFAVVC
                     SLAGVPFHVFRMPYFFIDFYEFGWFSASAYSSLYTFSSFGVFYWIIIILLCVFLLGFG
                     LLFLNRYIYAKQQKDTSVASEKTL"
     gene            1336..1824
                     /locus_tag="PLH17_03255"
     CDS             1336..1824
                     /locus_tag="PLH17_03255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOR20821.1"
                     /translation="MSIYQITVEDANKEKVILEKYRGRVLLIVNTATKCMFTPQYEGL
                     QYLYDKFHDKGFEILDFPCNQFANQAPQSDEEIAKFCSSEYKTTFPRFAKTNVNGPDE
                     NLLFTMLKKTLGGFFSPRIKWNFTKFLVDHNGKMVRRYGPNVKPSRIASDIEKLLQEK
                     GN"
     gene            1825..2937
                     /locus_tag="PLH17_03260"
     CDS             1825..2937
                     /locus_tag="PLH17_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20822.1"
                     /translation="MFLAWKEIKHSKGKFALIMAVVALVSYLVYFLTSLAYGLASSYT
                     NGITKWNADNIVLTEASNDNILMSTMSDVTNDDNDDYSLLTVAGEKAKLGIFMAVIKD
                     ETPAEGIEDTRADVYVFGIESAGFLNPISGLDLADNEIIADISIQEFGYEKGDTIIVT
                     GTDTNDLPLIKWTIVGFTAKSTYQTAPILYMNLKTWQDYRYYGRGAIPEEVTLFNAAV
                     VRGEVTNAPSVYVVAPIADFINSLPGYTAQVLTFSVMIGFLIVIVAFVLGIFIYVLTI
                     QKTPMFGVMKAQGISNKYIAGSVVSQTFILTAIGILIGLVLTLISGFFLAGTVPFAIN
                     ILFYVVITAAFIAFAIVGGLFSVRAVLKIDPLRAIS"
     gene            2950..3624
                     /locus_tag="PLH17_03265"
     CDS             2950..3624
                     /locus_tag="PLH17_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20823.1"
                     /translation="MKTLIEMQQITKDFLQGDDVVHALKETNFKAYEGELVAIIGPSG
                     SGKSTFLTIIGGLQTPTTGRVLINGQPFSQLNIKERSQVRFEKIGFILQASNLIPFLS
                     ISDQMFLHDRISRRGRNMERIEELYTRLDIKKLRNKYPSELSGGERQRVAIAKALYHD
                     PVVILADEPTASLDTNRAFEVIKLLAQQTKEQKKATIMVTHDERLTEFCDKVYVIVDG
                     VMTQKK"
     gene            complement(3627..4628)
                     /locus_tag="PLH17_03270"
     CDS             complement(3627..4628)
                     /locus_tag="PLH17_03270"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOR20824.1"
                     /translation="MKRTIKLINNMGMKVTLSPFGAAILDIVITMKDGCERMVTLRPK
                     SLADFASSSGYYGKTIGRVAGRLEKSHCHIDGGEYTVLETLPNNGLHGGNKGLSNAYF
                     DYINYRDPRGQIAIFSYQIPHLEDGLPGNLQVFVTYLLHLDRNRLEIRYEAKSDADTI
                     CNLTNHTYFNLDGQGTIIDHELMINATRFAEVDERLIPKRVVDVNPVMDFLRPKRIGD
                     YITARELSGAAQGYDHPYILNESNLQNSSASLTSSNNDLSLNMYTTYPCLVFYSGNYP
                     TNENMIPTKRMPKHGALALEPQYLPNAINNPFGQEKTGLLKAGSLYQESIIYEFIIK"
     gene            4717..5631
                     /locus_tag="PLH17_03275"
     CDS             4717..5631
                     /locus_tag="PLH17_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20825.1"
                     /translation="MDKILEVEHLKKYYGHIKAVDDISFYVEKGKLFAFLGPNGAGKS
                     TTIDIICTFLKPDNGSVRIDGHTIGKDDDKIRSAIGVVFQDGFLDDLLTVEENIKYRG
                     SFYGLKGKELQVAFERVVEAAGIKDFLKRQYKKLSGGQKRRSDIARALINTPKILFLD
                     EPTTGLDPQTRKKVWETIVKMQRDSNMTVFLTTHYMEEASEADYVIIIDNGKIAARGT
                     PYELRKHYANDSLRLEYLDQEAIEAILKGNNLSYTLHNGEAIIPLRATIDALAVLDLV
                     RDQVKSFEVLSGTLNDAFINITGREIRE"
     gene            5628..6488
                     /locus_tag="PLH17_03280"
     CDS             5628..6488
                     /locus_tag="PLH17_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOR20826.1"
                     /translation="MIIFALRNFKVFIRDRLSIFFSFLSVLIIFGLYTLFLNNAWASA
                     LPDNVTGGQALMDGWLMAGILAVVSFTTTLGAFGTMVDDKAKKIYKDFSSSPLKRSSL
                     VGGYIISAYLIGIIMSVFSLVFLDLYMLIRDGHILDFVETMQVLGLILLVTLANSAIV
                     FFITIFIKSMTAFATISTVVGTLLGFLAGIYMPVGNFPAAVQAVIKVIPLSHAASLFR
                     RIIMGDLADTVFYGAPIEIKNSFLDVMGVNFAFGDFVVLPWMQIVYLVATAILFYSLS
                     LIVVTFKKER"
     gene            6545..7132
                     /locus_tag="PLH17_03285"
     CDS             6545..7132
                     /locus_tag="PLH17_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR20827.1"
                     /translation="MKNKFLDLFLTFLKIGATTFGGGYAMIPLIRNDVVDKKRWLSEE
                     ELLEVLAIAESTPGPIAINVATYTGYKIRGFWGSLLATLGVVLPSFFIIFGISILYSY
                     IKDNQIIAAAFMGIKAAVALLILSAGLRMLKKMKKTWYSVPIMIIIMAALIIFDLLAI
                     SFSSVFLILGGALLGFFIYYLIPLISRQTKKGTDK"
     gene            7129..7695
                     /locus_tag="PLH17_03290"
     CDS             7129..7695
                     /locus_tag="PLH17_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOR20828.1"
                     /translation="MIYLLLFLEFLKIGLFSFGGGYGMISMMKETVLANNWLSEAQLL
                     DFIGIAESTPGPIAINMATFIGTSQGGMLGGAIATIGVILPSFVIILLLAIILKNVVK
                     SRPFQAIMSGIRPVIVGLIIGTGLIFTYSLIGLQDRSTFAFDYRSLVIFAILAVIYFA
                     YPRIFKKEVSPLLLIAFSVFFGIVLFAA"
     gene            complement(7742..8257)
                     /locus_tag="PLH17_03295"
     CDS             complement(7742..8257)
                     /locus_tag="PLH17_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOR20829.1"
                     /translation="MVTKKVVDLLNLQIQRELYSAYLYLDMALYYNDLGLVGHANWFE
                     IQAQEERDHALGFIRYLQHVGERPLLDVIDKPSLTYKDPREPLVEALKHEKFVTASIE
                     NILEVAKAEKDHLTVNFIQWYIAEQGEEEKNANDNILMYDFAKGDRAALLVIDGKHHA
                     RKYSPSIPDID"
     gene            8384..8896
                     /gene="msrA"
                     /locus_tag="PLH17_03300"
     CDS             8384..8896
                     /gene="msrA"
                     /locus_tag="PLH17_03300"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOR20830.1"
                     /translation="MKTLHERVIYLAGGCFWGIEAYFQQLKGVLDTEVGYANGDSENP
                     SYEDLVAHRANHAETVKVIYDNDIISLIKILEHFLRIVDPYSLNRQGHDVGIQYRSGV
                     YYESDGDKKIINSYFEEQNEDISRLFAVEVIPLKNFYRAEGYHQDYLLKNPFGYCHVD
                     MRKIKKEERK"
     gene            complement(8927..9871)
                     /locus_tag="PLH17_03305"
     CDS             complement(8927..9871)
                     /locus_tag="PLH17_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOR20831.1"
                     /translation="MIKKAIIVPLLLGGVMIVLGGTIAGISAAAAGGFAALSTAEPYS
                     ARVIEVPAADLNINVEEENNKIVINTSETALTVSITVYENNHETYVSSVDENDFTLVY
                     HNDVPWIKRIFYVPTSLRTMTITIPKNYAASFHINNTNGVIDVDDISLQGELALETAN
                     GLISVNNVDVTGNIDVRTTNSKIEITASSSLEDIAARSMNGYIVLDELTAVDIDAQTT
                     NGKIAAEDIAAENMKLATTNGQVTTADIDVATKIHLETTNGDIRGNVRGPSTDYDIDS
                     QTTNGSNNLAGFNSQVPSTGSKILYARCYNGAITITFN"
     gene            complement(9868..10482)
                     /locus_tag="PLH17_03310"
     CDS             complement(9868..10482)
                     /locus_tag="PLH17_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HOR20832.1"
                     /translation="MTKKEFLDKLRLEIRNLPYDEVEKTITYYEEMISDRVEDGATEA
                     EAVASLGTPESIARDLQAHQPFATIIKRKVEDYKSKRSPESTALIVVLLIVLFPFWFP
                     VLMSALGVIASFFAAIGGVIIALWAISVSIGIGGIAMIIASTFGFASSYIPTGFFSIG
                     LGIALIGLSIIAIIGSLYATKGIIKLLESLSHTIKKMFVRRTNA"
     gene            complement(10484..10804)
                     /locus_tag="PLH17_03315"
     CDS             complement(10484..10804)
                     /locus_tag="PLH17_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOR20833.1"
                     /translation="MDIQLKRGLLEVCVLAELVKGDSYGYQIVKDLSEVVEISESTLY
                     PILKRLEMSECLRVFSVEHNGRLRRYYRITDAGRKRIKEFLNEWEEVTNVYTFISGIH
                     KGEN"
     gene            complement(10920..12407)
                     /locus_tag="PLH17_03320"
     CDS             complement(10920..12407)
                     /locus_tag="PLH17_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20834.1"
                     /translation="MYLLPIVNLASNSAFNSGDFNVTWSIVFGLLVVLAILLLSNYLR
                     RVIPFLRKSLLPVAFIGGAIGLGLKIAFQYSELAVTGLNVYTDGFKAMLQVITYHALA
                     VGFIALSLKVVSKERAKSKEMGRDSVKGGIIIIGSYLLQAIIGVTVTILLSLVFTTIF
                     PYAGIILPLGFGQGPGQAMTWGSTYEALGFAGGTDFGITISTMGFLSAAVGGVIYLNY
                     AAKKGFVKRRDENENAPQSDEMVEKQGEVPLSESIDKLTIQVALIGIVYLLTFAIIYP
                     LSFIPVAFIKNTIVPLLFGFNFIVGTMIAMLVKKLLAALQKRKIIKKQYINNFMLERI
                     AGLAFDMMIISSIMAIDIRNLADVGLIVTLAVICVIGAFATFFYVKYMTFKAYPGYKH
                     QGFVTFYGNLTGTASDGIALLREIDPTFKTPSADSLVYGSTVAIALGFPVLLIVGYIY
                     KGGNTLASVPLWVSYGTLIAYFVAINLLFFFLLKRHKKKDAQVRS"
     gene            complement(12373..>12846)
                     /locus_tag="PLH17_03325"
     CDS             complement(12373..>12846)
                     /locus_tag="PLH17_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20835.1"
                     /translation="FCKNCDFEGHYDESGHFTIDKKPYQLHEIARPMIDDYEKYVLAH
                     INENFSEDVDIKVCWGNKRRHYYAACTFGLSRDGITIVGKKVDFTIPYSDLRGYGRQQ
                     KRKIVLYSYSLPTIIIKMKPKVSIYQYYITIKIFENIKRKGINNYVPLTHRELGF"
BASE COUNT         3270 a         2936 c         2982 g         3658 t
ORIGIN      
        1 tataatgtca ccgatccgcg ctttacaacc tacacttctg ctgtaagcag ctccatgctt
       61 cttcctcaac tttaccgctt tgtcgacgat gttaatcccg ccaaagaagg agcttatgat
      121 tatatctcaa ctttcatgaa tacaaccgcc tctgaatgcg ccgccctcga tgttaaaagc
      181 tcgacatggt caaccttaaa atcaggttat gaaattcttt tagacgagtc gaaaaactat
      241 atttacgagc attccgaaaa cgatgtttta attgccgcga tggttgaacg ctatgccgtg
      301 attatcaaca agtacggata cgacaatttc ttgaccgacg ccctcggcaa tccggtcttg
      361 atggctctta atagcgatga tggtatcgat ctactcggta gcgaagcggc catcgccgcc
      421 gcgataatat cctttgccgg catcattttt atctcgcttg ccggtttcct cttctaccaa
      481 aagacgaaaa aacagatact ttaatttaaa ttaaaaatcc ttacattttg ccaattggct
      541 cgatacaatt aagcatagga taggctatga gcaaatattt actgggcaag atatcaatca
      601 actttgccgt ttttatctgc gccatggtcg gcgtcatcgc cacctcattt ggtgtgtcag
      661 gactcatgac cggtgtctcg attctttact acacggtcca gagcaatatt tggattggtt
      721 tgacgagcct tacctttgct attttacaaa taatcggggt taagaaatca agtgttgtga
      781 taaccggttg gctctatgtt acaaaatacg tttttgtggt ggccatcact ttgacgatgg
      841 tcatcttctg gctgctgctc gctccgacta ttcagctgcc ttcttatctt ctttcgccga
      901 gcaatctctt cgctcataca cttaccccaa tcttgtcgct tgcgtctttc tccttttttg
      961 attctcgaga ctataaattg tcgaaaaaga gttctttttt ctctttagcg acgccacttt
     1021 actattttat ctttgctgtt gtctgttcgt tagcaggcgt gcctttccac gttttccgga
     1081 tgccttactt ctttatcgac ttctacgaat ttggctggtt ctcggcttcc gcttattctt
     1141 cgttatacac cttttcatcc tttggcgttt tctactggat catcatcatt ttattatgtg
     1201 ttttccttct gggttttggc ttactgttcc tgaatcgata catttatgct aagcagcaaa
     1261 aagatacgag cgttgcgagt gaaaagacgc tgtaaatact ttaaaattga tatgtttata
     1321 acaggaggaa aatagatgag catctatcag atcaccgtcg aagacgccaa caaagaaaaa
     1381 gtcattctag aaaagtatcg cggtcgcgtt ttgctaattg tcaacacggc gacaaaatgc
     1441 atgtttaccc ctcagtatga aggacttcaa tacctctatg acaaatttca cgataaaggc
     1501 tttgaaatcc ttgattttcc gtgcaatcaa tttgccaatc aggcgccgca aagcgatgag
     1561 gaaatcgcta aattctgcag ttcggaatac aagacgactt ttccgcgctt cgctaaaact
     1621 aatgtcaacg gaccagatga aaatctactc tttacgatgt taaagaagac gctgggcggc
     1681 tttttttcgc cgcgcatcaa gtggaacttc acgaagtttc tagtcgacca taacggcaag
     1741 atggtgcgcc gctacggacc gaatgtcaaa ccatcacgca tcgcttccga tattgaaaaa
     1801 ttattgcagg aaaaggggaa ctaaatgttt ttagcttgga aagaaatcaa acactccaaa
     1861 ggcaaatttg ccctgattat ggcggtcgtc gccctcgtca gttatctcgt ctactttttg
     1921 acttcgctgg cctatggtct cgcctcttcg tatacaaacg gcatcaccaa atggaatgcc
     1981 gataatatcg tcttaaccga agcttcaaat gacaatattc tcatgtcgac aatgtctgat
     2041 gtcaccaatg acgataatga cgattacagc ctcttaaccg tcgcaggcga gaaggcgaaa
     2101 ctcggcattt ttatggccgt catcaaagat gaaacgcccg ccgaaggcat cgaagacacg
     2161 cgagctgatg tctatgtttt cggcatcgaa agcgccggct ttcttaaccc tatttccgga
     2221 cttgacctcg ccgataacga aattatcgcc gatatctcga tacaagagtt cggctatgaa
     2281 aaaggcgaca ccatcatcgt cacgggaacc gacaccaatg atctcccttt aatcaagtgg
     2341 acaatcgttg gcttcaccgc gaaatcaact tatcaaacgg ccccgattct ctatatgaat
     2401 cttaagacgt ggcaagatta tcgctactat ggccgcggcg cgattcccga agaggtgacg
     2461 ctatttaatg ccgcggtcgt gcgcggtgaa gtgactaatg ccccatctgt ttatgtcgtt
     2521 gctcccatcg ccgattttat caatagcctg ccgggctata ccgcccaagt tttaactttt
     2581 agtgtgatga tcggctttct gatcgtcatc gtcgcctttg tcctcggcat ttttatctat
     2641 gtgttaacga ttcagaaaac gccgatgttc ggagtcatga aagctcaagg catatcaaac
     2701 aaatacatcg ccggatcggt cgtcagccaa acctttattt taacggccat cggcatttta
     2761 atcggtcttg ttctgacgct tatctccgga tttttcttag ccggaacggt gccttttgcc
     2821 atcaatatcc tcttctatgt cgtcatcacg gccgccttta tcgcttttgc gatcgtcggt
     2881 gggctcttct cagtgcgcgc cgtcttgaag attgatcccc tccgggccat cagctagagg
     2941 taacaaatca tgaaaacatt aattgaaatg caacaaatta cgaaagattt ccttcagggc
     3001 gatgatgttg tccacgcttt aaaagagaca aattttaagg catatgaggg agaattagtt
     3061 gccatcatcg gaccatccgg ttccggcaaa tcgacatttc tgacgatcat cggcggtttg
     3121 cagacgccga ctaccggccg cgtcttaatc aacggacagc ccttcagtca acttaacatt
     3181 aaggaacgct cgcaagtgcg tttcgaaaag atcggcttta ttctccaagc ctcaaatctc
     3241 attcccttct tgtctatctc tgaccagatg tttctccatg accgcatcag ccgccgcggc
     3301 cgcaatatgg aaaggattga agaattgtat acgcgtctcg atatcaaaaa gcttcgcaac
     3361 aaatatccaa gtgaattatc cggcggcgag cggcagagag tcgcgattgc caaggccctt
     3421 tatcatgacc cagtcgtcat cctcgccgat gaaccgacgg ccagcctcga cacgaaccgg
     3481 gcttttgaag tcatcaaact gctggcccag caaaccaaag aacaaaaaaa ggcgaccatc
     3541 atggtcactc acgatgagcg cctcaccgaa ttctgtgaca aagtctatgt catcgtcgac
     3601 ggcgtgatga cgcaaaagaa atagacttat tttatgataa attcgtagat tatcgattct
     3661 tggtataatg aaccggcttt taacaggccg gttttttctt ggccgaacgg attgttgata
     3721 gcgtttggca aatactgcgg ttcaagggcg agagctccgt gcttcggcat tctttttgtc
     3781 ggaatcatat tttcattggt cggatagttt ccggaataaa aaacgaggca cggataagtg
     3841 gtgtacatat ttaaagataa atcattgttt gaactggtaa gcgacgctga agaattttgt
     3901 aaattcgatt cattgaggat ataagggtgg tcataacctt gggcggcgcc ggaaagctcg
     3961 cgagcggtga tgtaatctcc gattcgcttc ggtcttagaa aatccataac tgggttgacg
     4021 tcgacgactc gcttcggaat tagacgctca tcgacttcag caaatcgcgt ggcattaatc
     4081 atcagctcat gatctataat cgttccctgg ccatcgaggt taaaatatgt gtgattggtg
     4141 agattacaaa ttgtgtcggc atcacttttt gcctcatagc gaatttcgag gcgattgcgg
     4201 tcaagatgga gcaaatacgt gacaaaaacc tgcaaattgc cgggtaatcc gtcttctaga
     4261 tgcggtatct gatatgaaaa aatggcaatt tgtccacgcg gatcgcgata atttatatag
     4321 tcaaaatatg catttgataa acctttgttg ccgccgtgta aaccgttatt tgggagagtt
     4381 tccagcaccg tgtattcgcc tccgtcgatg tggcagtggc tcttttcaag gcgcccggct
     4441 acgcggccga ttgtctttcc ataatagccg ctagaagagg caaaatcagc gagagatttg
     4501 ggccgtaacg tcaccattcg ctcgcatcca tctttcatcg ttataacgat atcgagaatg
     4561 gcggctccaa aaggcgaaag agttaccttc attcccatat tgtttataag ttttatggtt
     4621 cttttcatcg cattattatc atagcgcaag tacgatataa tttgcgaaaa ataaaacact
     4681 tattataata aagcatagtt atggcggggc aaccatatgg acaaaatttt agaagtcgag
     4741 catctcaaga aatactacgg ccacataaaa gctgtagatg atatttcatt ttacgtcgaa
     4801 aaaggtaaat tatttgcttt tctcggccca aatggcgcgg gcaaatcgac gacaatcgat
     4861 atcatctgta cttttttaaa accggacaac ggttcggttc gcatcgatgg gcacacgatt
     4921 ggaaaagatg atgacaaaat tcgctctgca atcggcgtcg tctttcagga tggctttctc
     4981 gacgatttgc tgaccgtcga agaaaacatc aaataccgcg gaagtttcta cggtctaaaa
     5041 ggaaaagaac tccaggtagc ctttgagcgc gtcgtcgagg ccgcgggcat caaagacttt
     5101 ttaaaacgtc agtacaaaaa gctttctggc ggtcaaaagc gccgctccga catcgccagg
     5161 gctttgatta acactcccaa aattctcttt cttgatgagc cgacgaccgg gcttgatccg
     5221 cagacgcgaa aaaaagtctg ggaaacaatt gtcaagatgc agcgcgattc caatatgacc
     5281 gttttcttga ccactcatta tatggaagag gcttcggaag ctgattacgt catcattatc
     5341 gataacggta aaatcgccgc tcgcggaacg ccttatgaac tgcgtaagca ctacgccaat
     5401 gatagcctgc gcctcgaata ccttgatcag gaggccatcg aagcgatact taaaggcaac
     5461 aatctgagct acactttgca caatggcgaa gccatcattc cccttagggc gacgattgac
     5521 gccttggctg tcctcgattt agtgcgcgac caagtcaaga gttttgaagt gttatcaggc
     5581 acccttaatg atgcctttat caacatcacg ggaagggaga ttcgcgaatg atcatctttg
     5641 cccttcgcaa ttttaaagtc tttattcgcg accgcctttc gatcttcttt tcgtttttgt
     5701 cagttctgat tatttttgga ctatatacat tgtttcttaa taacgcctgg gcttcagctc
     5761 tgcccgataa tgtcactggc ggtcaagcct tgatggatgg ctggctgatg gccggtattc
     5821 tcgccgtcgt ctcatttacg acgacgctcg gcgctttcgg aacgatggtc gatgacaagg
     5881 ccaaaaaaat ctataaagac ttctcatctt cacccttaaa gcgttcttcg ctcgtcggcg
     5941 gatatattat ctcggcctat ttaatcggta tcatcatgag cgttttctcg ttggtttttc
     6001 ttgatttata catgctcata cgcgatggcc atattctcga tttcgtcgag actatgcaag
     6061 ttctgggatt gattttgctc gtcacgctcg ccaatagcgc catcgtcttt tttatcacga
     6121 tcttcatcaa gagcatgacg gcctttgcca cgatcagcac ggtcgtcggc actctcctcg
     6181 gctttttggc cggaatttac atgcccgtcg gcaactttcc ggccgccgtc caggccgtca
     6241 ttaaagtcat ccccctctct catgccgcct ccttgttcag acgaattatt atgggcgatt
     6301 tagccgatac cgttttttat ggcgccccca tcgaaatcaa gaattccttt ttggatgtga
     6361 tgggtgtcaa tttcgccttt ggcgatttcg tggtattgcc gtggatgcaa atcgtctatc
     6421 tagtggcgac ggccatcctc ttctatagtc tttcactcat cgtcgtgact tttaaaaaag
     6481 agcgctgagg gacttgttat tacgatggcg ttttgccatc ttttatgcta tgttattaac
     6541 ttgtatgaaa aacaagtttt tagatttatt tctcaccttt cttaaaatcg gtgcgacgac
     6601 tttcggtggc ggctatgcga tgattccgct gattcgtaat gacgtcgtcg acaaaaagcg
     6661 gtggctgagc gaagaagaac tcttggaagt tttggccatc gccgaatcga cgcccggtcc
     6721 catcgccatc aatgtcgcga catacaccgg ttataaaatt cgcggattct ggggcagcct
     6781 cctggcgacg ctcggcgtcg tcctgccttc ctttttcatc attttcggaa tttcgatttt
     6841 atactcctat atcaaggata atcaaataat cgccgcggct tttatgggca ttaaagcggc
     6901 ggtggcactt ttaattcttt cggccggcct cagaatgctc aaaaagatga agaagacatg
     6961 gtattcggtt ccaatcatga ttattatcat ggcagctctg attatctttg atttgctggc
     7021 gattagcttt tcctccgtct ttttgattct gggtggagcg ctcctcggat tttttatcta
     7081 ttatcttatc cccttaatat cacggcaaac aaaaaagggg actgacaaat gatatacctc
     7141 ctcctttttt tggaattttt aaaaatcggt ctcttctctt ttggcggcgg ttacgggatg
     7201 atctcgatga tgaaagagac ggtcctcgcc aataactggc tgagcgaagc ccagttgctc
     7261 gattttatcg gcatcgccga atcgacaccc ggtccgatcg cgattaacat ggcgaccttt
     7321 atcggcactt cgcaaggcgg aatgctcggg ggagcgattg cgaccatcgg cgtcattttg
     7381 ccgtcctttg tcatcatttt actcttagcc atcatcttga aaaatgtcgt caaatcgcgg
     7441 ccctttcaag cgattatgag cggcattcgc ccggtcattg tcggacttat catcggcacc
     7501 ggtttgattt ttacttatag tctgattggt ttgcaagatc gttcgacctt tgccttcgat
     7561 tatcgaagtc tcgtcatctt cgctatcttg gccgttatat atttcgccta tccgcggata
     7621 tttaaaaagg aagttagccc tttgctcctc attgccttct ccgttttttt tggcatcgtt
     7681 ttattcgccg cataaaaaag ccccacctaa taaggtgagg cgatacataa atcgattctt
     7741 cttaatcgat atccggaatt gacggagaat atttgcgagc gtggtgtttg ccgtcgatga
     7801 ctagtaaagc ggcgcgatca cctttcgcga aatcgtacat cagaatgtta tcgttggcgt
     7861 ttttctcttc ttcgccttgt tcggcgatgt accactgaat aaaattgacg gtcagatgat
     7921 ccttttcggc tttagcgact tcgaggatgt tttcgatgct ggcggtgacg aatttctcgt
     7981 gtttgagcgc ttcgactaag ggttcgcggg gatctttata agtgagggat ggcttatcga
     8041 tgacatcgag aagcggcctt tcaccgacgt gctgcagata gcggatgaaa ccgagggcgt
     8101 ggtctctttc ttcctgggcc tgaatctcaa accagttggc gtggccgacg agaccgagat
     8161 cgttgtaata aagcgccata tcgagataga gataggcgga gtagagctcg cgttgaattt
     8221 gtaaattgag taaatcaaca actttttttg taaccatgta attacctcct aggtgttact
     8281 tttctaacat ctatatctta gtcgatatgt aactatgtta aaagttattt gctcgcgatc
     8341 attactattt tcttatcgca tcgttcatga aataattatg cttatgaaaa cgttacatga
     8401 acgcgtcatc tatttagccg gaggctgttt ctggggcatc gaagcctatt ttcagcagct
     8461 taaaggcgtc ctcgacaccg aagtcggcta cgccaacggc gatagcgaga atccatctta
     8521 tgaggatttg gtggcccatc gcgcgaatca tgccgagacc gttaaagtca tctatgataa
     8581 tgacattatt tcgcttatca aaattctcga acactttctg cgcatcgtcg atccctattc
     8641 gcttaatcgg caaggtcatg atgtcggcat tcagtatcga agcggtgtct actatgaaag
     8701 cgatggagat aagaaaatta tcaattccta ttttgaagaa caaaatgagg atatctcccg
     8761 attgtttgcc gtcgaggtga tccccttaaa aaacttttat cgggcggaag gatatcacca
     8821 ggattatctc ttaaaaaatc cctttggcta ttgccacgtc gacatgcgga agataaaaaa
     8881 agaggagcgc aagtgaagcg ttccttttta attatttaga gtttatttaa ttaaacgtga
     8941 tggtaatcgc cccgttatag cagcgggcgt aaagtatttt gctaccggtc gacggaactt
     9001 ggctattaaa gccggcgagg ttgttcgagc cgttggtcgt ctggctgtcg atatcataat
     9061 cggtcgacgg tcctctgaca ttgccgcgaa tatcgccgtt cgtcgtctcg aggtgaattt
     9121 ttgttgcgac atcgatgtcg gctgtcgtca cttgtccgtt ggtcgtcgcg agtttcatgt
     9181 tttcggccgc gatgtcttcg gcggcgattt tgccgttagt cgtctgcgcg tcgatatcga
     9241 cggcagtcag ctcatcgagg acgatatagc cgttcatcga gcgggcggcg atatcttcta
     9301 gtgatgaaga agcggtgatt tcaatcttag agttcgtggt ccggacatcg atgtttccgg
     9361 tgacgtcgac attgttcacg ctgatcagac cgttcgcggt ctcgagcgcc agttcgcctt
     9421 gcagggagat gtcgtcgaca tcgatcacgc cgttagtatt attaatatga aacgaagccg
     9481 cgtaattttt ggggatggtg attgtcatcg tgcgcaaact cgtcggaaca tagaagatgc
     9541 gcttaatcca cgggacatcg ttatgataga cgagagtaaa gtcgttttca tcaacgctcg
     9601 aaacataagt ttcgtggttg ttttcgtaga cggtaattga gacggtcagc gctgtttccg
     9661 aagtattgat aacaattttg ttgttttctt cttcgacatt gatgttgaga tcagcggcgg
     9721 gaacctcgat tacgcgggcg gaatagggct cggcggtcga taaggcggca aaacctccgg
     9781 ccgcagcggc gctgatgccg gcaatcgtgc ctccgagaac aatcatcaca cctccgagaa
     9841 gaagcgggac gatgatggct tttttaatca tgcattggtc ctcctgacaa acattttttt
     9901 aatcgtgtgc gatagcgatt caagcaattt gatgatgccc tttgtggcgt agagcgaacc
     9961 gatgatggcg atgattgata aaccgattaa ggcgatgccg agaccgatcg aaaaaaagcc
    10021 ggtcggaata taggagctgg caaagccaaa tgtcgaggcg atgatcatgg cgataccgcc
    10081 gatgccgatt gagacgctaa tcgcccataa ggcgatgatg acgccaccga tggcggcgaa
    10141 gaagctggcg atgacgccta aagcgctcat caagaccgga aaccagaagg gaaagagaac
    10201 aatgagcaat acgacgatta aagcggtgct ctcgggactc ctttttgatt tataatcttc
    10261 aactttgcgc ttgatgattg tcgcaaacgg ctgatgggcc tgcaaatcgc gggcgatgct
    10321 ctccggtgtg ccgagtgagg cgacggcctc agcttcggtg gccccgtctt cgacgcggtc
    10381 ggaaatcatc tcttcgtaat aggtgatcgt tttttcgact tcatcatacg gaagattcct
    10441 gatttcgagc cgaagcttat cgagaaattc tttttttgtc atgctaattc tcccccttat
    10501 gtattccgct tataaaggta taaacattgg ttacttcttc ccattcattg agaaactctt
    10561 tgatgcgttt gcggccggca tcggtgatac gatagtagcg gcgcaggcgg ccgttatgct
    10621 caaccgagaa aacgcgaagg cactcgctca tttcaaggcg ttttaaaatc ggataaagcg
    10681 tcgattcgga tatttccacg acttcggaaa gatccttgac gatttgatat ccatacgaat
    10741 cgcccttgac cagctcggcc aggacgcaca cttccagaag accgcgtttt aattgaatat
    10801 ccatatgccc tcctgacaat actatacaac gcgtagtatt atgtgtcaaa taatattttc
    10861 caattaatga cataaataaa aaagcccgaa ctaatcgggc ctattttgat atttgtttat
    10921 tagcttcgca cttgcgcatc tttcttttta tgtcttttta aaaggaaaaa gaagaggaga
    10981 ttgatcgcga caaaataagc gatcaaggtg ccgtaagaga cccataaagg cactgaggca
    11041 agggtatttc cgcctttgta gatatagccg acgatgagca agacgggaaa gccaagagcg
    11101 atggcgacgg tgctgccata gacgagtgag tcggcggatg gcgtcttaaa ggtcggatca
    11161 atttcgcgga ggagggcgat accgtcggaa gcggtgccgg tcaaattgcc gtaaaaggtc
    11221 acaaaacctt gatgtttata gccaggatag gctttaaatg tcatatattt tacatagaag
    11281 aatgtagcga aggcgccgat gacgcagata acggctaggg tgacgattaa accgacatcg
    11341 gcgagattgc gaatgtcgat ggccatgatc gaagagataa tcatcatatc aaaggcgagg
    11401 ccggcgatgc gctcgagcat gaagttgtta atatattgtt tcttgataat ctttctcttt
    11461 tgcaaagcgg ctagaagctt tttgacgagc atggcgatca tcgtgccgac gatgaaatta
    11521 aagccaaata ataacgggac gatcgtattt tttataaagg cgaccggaat aaacgaaagc
    11581 ggatagatga tggcaaacgt cagaaggtag acgatgccga tcagcgcgac ttgaatcgtc
    11641 aatttatcga tggattcgga gagtggaacc tcgccttgtt tttcgaccat ctcatcgctt
    11701 tgtggagcat tttcattttc atcgcggcgt ttaacaaaac ctttttttgc ggcgtagttt
    11761 aggtagatga caccgccgac ggccgccgat aaaaagccca tcgtcgagat ggtgatgccg
    11821 aagtcggtac ctccggcaaa gccgagggct tcataagtgc ttccccaagt catcgcctgg
    11881 cccggacctt gcccaaaacc aagtggtaaa atgatgccgg cgtaagggaa aatagtggtg
    11941 aagacaagac ttaataaaat ggtgacggtg acgcctataa tcgcctgtaa taggtacgaa
    12001 ccgatgatga tgatgccgcc tttgacgctg tcgcggccca tctctttgga ttttgcccgt
    12061 tccttgctga caaccttgag gctaagagcg ataaaaccta ctgctaaggc atggtaggtg
    12121 attacttgga gcatggcttt aaaaccatcg gtgtagacat tgaggccggt gacggcgagc
    12181 tcgctatatt gaaaagcgat tttgaggccg agcccgatcg cgccgccgat gaaggcgacc
    12241 ggcaaaagcg atttacgtaa gaaaggaatg acccttctta aataattgga gagcagcaaa
    12301 atagccagga cgacgagaag gccaaagaca atcgaccacg tgacattgaa gtctccggag
    12361 ttaaaggctg agttagaagc caagttcacg atgggtaaga ggtacataat tattaattcc
    12421 tttccgtttg atgttttcga atattttaat agttatatag tactggtaaa ttgatacctt
    12481 tggtttcatc ttgatgatga ttgtcggaag cgaatacgaa tagaggacga tcttgcgctt
    12541 ctgctggcgg ccatatccgc gcaagtcgct atatggaatc gtgaagtcga ctttcttgcc
    12601 gacgatggtt atcccgtcgc ggcttaagcc gaatgtgcac gccgcgtagt aatggcggcg
    12661 cttgttgccc cagcacactt ttatgtcgac gtcttcactg aaattctcat tgatgtgagc
    12721 gagcacgtat ttttcatagt catcgatcat cgggcgggca atttcgtgca attggtatgg
    12781 ttttttatct atcgtgaaat gaccgctttc gtcataatgg ccttcaaaat cgcaattttt
    12841 gcagaa
//
LOCUS       DAOSSL010000024        12796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_4922_length_12796_cov_10.698297, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000024 DAOSSL010000000
VERSION     DAOSSL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_12796_cov_10.698297"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLH17_03330"
     CDS             <1..189
                     /locus_tag="PLH17_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20836.1"
                     /translation="LKMEKVDKDSYAINGVFRANNKTSEQWLNLFGDFNLLKQNGFQR
                     APGLPADRLFLLKKPGNC"
     gene            complement(186..>1559)
                     /locus_tag="PLH17_03335"
     CDS             complement(186..>1559)
                     /locus_tag="PLH17_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20837.1"
                     /translation="SDPYDWKVKPLNIGSINVGASIGFFGNIGNVSINPTTAVIGTVK
                     SFYLENVEVTNKVNDSAVGIVAGYNNGNMEAIGVKNAKISLGTTNIEVMSSYSLIGKI
                     GPYVSWDSLPEDLAGGDLLIDTNFPSFTTKAANTVTAVPDSATDTAFFIGSVTATSVD
                     IKGSLFKYNSKVTVDTSATSPNTYTADSGNTTLVTSSNWTSHVTPDFYNRTLPNDTKM
                     ATFGITPPSYSSATLSFSNTSNITLYDGTTFPVPKNSIWFKPIKGGVSALVFARQRNA
                     REPGKLSVYYFKREGSAITNFGEVRFQIGQVGNKSQVYFDFEILQATLDDDYEFIISA
                     SNTNNDETNGFLYLMLAGSDEASGAQASAMKSVDYVYRTQSGALENVGAEDYMMKRTL
                     LRFNGTYSGTAYYNTKDYPNIDGKVYYAVVTSGGLHITDYVTTGTVGVSTTYTSGMFP
                     GRDEEFP"
     assembly_gap    1560..1659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1660..1846)
                     /locus_tag="PLH17_03340"
     CDS             complement(<1660..1846)
                     /locus_tag="PLH17_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20838.1"
                     /translation="MRKRKNIILIGVLTGGLFLSLVATSVAWFSTMTDFQTAINGRST
                     SNYYAGGDGSQNNPFIIK"
     gene            complement(1858..2580)
                     /locus_tag="PLH17_03345"
     CDS             complement(1858..2580)
                     /locus_tag="PLH17_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20839.1"
                     /translation="MKLTKRFKTTILVYANLAAAMVGIGAFTYTTLAWFAQNLTRQVH
                     MESVRVTIPGFAVTGFDCYEVTNLVKGETNTAITFSSQTVTSLATYDPQWQMNSTFSP
                     AIVVHVQFTYDAAEIVTFSATTDHTTFSSGTTGSGETDDNYASNALQITPTGGPSSGL
                     GESWTSASLTYINADADSFVTIGATPSKVTSLDLAAINPGDTETWFVLEYSQAVMDYI
                     DNARIGNDRDVIYRDDIVYLVA"
     gene            complement(3260..4519)
                     /locus_tag="PLH17_03350"
     CDS             complement(3260..4519)
                     /locus_tag="PLH17_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOR20840.1"
                     /translation="MIPGNYKKRIIEKGIEDKMKYSGAVVIEGPKWTGKSTTAELYSK
                     TIIKLQNPITKKQYQTLATISKDEVLSGEKPILFDEWQEVPEIWDFIRLDIDDNKYKG
                     AYLLTGSTKKQNINISHTGTGRINSIYMRPMSLFESGDSIGSVSLADLFEGKKIQVAK
                     SKTSINDIAHYICRGGWPGSLKLPLKEQLAIPKDLLESIISKDVDEVDGTKKDKDKIR
                     KLIRSYARNISTLATSKTIYKDQENEGISIDYKTYDTYTNALQRLYIIENLKAWSPAI
                     RSASTIRTSDKKQFVDPSIAVAALGLSVDKLKQDFETFGFFFESICTRDIRVYAQSLD
                     GEVYHYNDSSGLEVDLIIELRDGRWAGIEVKLGENEVEKAASNLIKLAKIHKTKKPSF
                     LMILTNTELCYQRTDGIYVIPLGLLKH"
     gene            complement(4914..5357)
                     /locus_tag="PLH17_03355"
     CDS             complement(4914..5357)
                     /locus_tag="PLH17_03355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20841.1"
                     /translation="MEFNQNFTIIKGDTLTLKLIETCVGDDKVIPFYYYEIYLNSISS
                     MIGKISIRIGYNEHSYINGNVGYEIDEPFRGNGYAKMAVMMTYSVARLHGMTHLVISC
                     DEDNYPSKSVINKLGDIHIETITPPNNFVFYYDGIPKHSIYRVDI"
     gene            complement(5369..6718)
                     /locus_tag="PLH17_03360"
     CDS             complement(5369..6718)
                     /locus_tag="PLH17_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOR20842.1"
                     /translation="MTMLERKIYKQLLSWKNENGKTALLIEGARRVGKSTVVEAFARN
                     EYKSYMLIDFSIASDEIKSLFKKYASNLDDFFFFLTSITGITLYERNSLIVFDEVQLF
                     PQARQLIKHLVHDNRYDYIETGSLLSIKRNVESILLPSEERTIKMYPLDFEEFCNALG
                     RVDVISLIKKHFETLKPLNEIHSTIMKLFRQYLLIGGMPQAVMEFLNTNDYTKVDRIK
                     RDILKLYKNDISKYAKRYEHKVLSIFDEIPSQLSKREKKFMLSSLSKDARFRDYEDAF
                     TWLDEAMITNVCFNASDPNFGLSLNRDRLTLKMYMADTGLLLSQAFSENDEMHQEVAK
                     AIIQDRLEVNNGMIFENIVAQMFTAENKKLYFYSRADNQDRSNTMEIDFLIIDQSKPT
                     KISPVEVKSGKNYTTSSLDKFSIKFKDKLGKKYIIHTKDLSQKDDVIYLPVYMTMFL"
     gene            complement(7096..7180)
                     /locus_tag="PLH17_03365"
     tRNA            complement(7096..7180)
                     /locus_tag="PLH17_03365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7144..7146),aa:Leu,seq:cag)
     gene            complement(7220..>8212)
                     /locus_tag="PLH17_03370"
     CDS             complement(7220..>8212)
                     /locus_tag="PLH17_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOR20843.1"
                     /translation="LTRGLIIAGIALVIVIIALSAFVNVMFKLALNKRGDGSPALRYL
                     QAEDFVGLVGEPIKFNGNKKQVLRGYLYSNQQFKTFKELVIFTHGIGAGHTAYTTEIN
                     RLAKEGFLVLAFDYTGCALSSGRAMKSLIQPLVDLDYALSYIESRSDIKDLKRYIVGH
                     SWGGYVAMNSVLLKKHRVDKVVSLSAFLSPSLVTIANRPALYPLYPFIYMHGFWRYGR
                     YATYNGVKALRRAKIPMLLIHGREDRVIKAKNSIDRFAKVAKAKANIELLYIEDRGHL
                     PYLSKRAEDYLYQVQKDKAIYDRKNPVLDYEINYELITEEDEGVMKKIVIFLKK"
     assembly_gap    8213..8312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8313..10642)
                     /locus_tag="PLH17_03375"
     CDS             complement(<8313..10642)
                     /locus_tag="PLH17_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOR20844.1"
                     /translation="MAQYQKIPDEIKSLPLQKKLELLYGDGFWKVRGIEDFNIKPFLV
                     ADGPHGLRKQVDKGDQLGIMAAFPATCFPTASLLACSFDQDLIFQMGQAIAREALHQH
                     VAVVLGPGVNIKRNPLCGRNFEYFSEDPLLSGKMGAAFIKGVQSRGVGTSLKHYAANN
                     QETNRLTINAVIDARALHEIYLKPFEIAVKEAKPYTIMCSYNRINGTYSSENNWLLND
                     VLRNKWGYRGLVVTDWGAINDDFLARKSGTDLEMPGIGRRDINLKCGIKKGLLDEDDV
                     NVCVNNIYELHLKVKELEYGNDGHYEEHFSLARKIAAESSVLLKNNGVLPLKSFDNVA
                     ILGAFAALPRYQGTGSSKVNPFRLVSFIEALKNENIKANYALGYKSNGDEIDDKFEQE
                     AIELAKQKDIIVIFAGLPDAYESEGFDRSKMSLPDSHLSLIEKVVALKKKVIIILAGG
                     SVMELPFADQVDGLLLTYLAGEACGPAVVDVLLGRVNPSGKLPETWPLTYKDVPSGNF
                     FPGDGTNVLYKESIYVGYRYYDTFNIAVRYPFGYGLSYTTFTYANLVLKYEFSEGKIR
                     YAVSFDVTNTGATSGAEIAQLYVASKAAGVYKPSHELRGFAKVRLEPNETKRVTIYID
                     QDDLRIYDLTTHEYFLEKGRYVFEVGASSRDLRLSKEVLIEGEDASHLVDDDLGDYYA
                     PHHPLTVSDEQFEKILQDRIPVARDRKIRPFSKNSTLKDIEDTMVGKIITKTLHNMAN
                     KMTGEDIEATRRMFIESALTMPLRGYTMSGMMSNRA"
     gene            complement(10702..11502)
                     /locus_tag="PLH17_03380"
     CDS             complement(10702..11502)
                     /locus_tag="PLH17_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOR20845.1"
                     /translation="MSLPLTQADWIILIIGLSMPFVGTALGASMVIFAKNGIKPWLQK
                     VLLGFASGVMVAASIWSLIIPALEAPVNGGIVSVVIGFALGMGFLLLLDTITPHLHIN
                     SEKPEGVKTKKISRTSMMVFAVALHNVPEGIAVGVTFAGALSGNAGITFMGALALAIG
                     IAIQNFPEGAIVSLPLRLEGHSRTKSFLIGGLTGIIEPVAAILTILLTELMVPILPYL
                     LAFAAGAMIYVVIEELIPQSQEGAHSNLATVGFMVGFIVMLLLSVLIG"
     gene            complement(11510..>12796)
                     /gene="eno"
                     /locus_tag="PLH17_03385"
     CDS             complement(11510..>12796)
                     /gene="eno"
                     /locus_tag="PLH17_03385"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOR20846.1"
                     /translation="VFAREVIDSRGNPTIEVEVQTYCGAFGRAIVPSGASTGEREALE
                     LRDGDKKRFGGKGVLKAVENVNKILAPAMVGMDVREQKVIDAKLLALDGTKFKSNLGA
                     NALLGVSLAAVKCAADFNHVPLYEQIALNAGEVGVTLPVPMMNVINGGSHADSSVDFQ
                     EFMIMPVGAPSVREAIRWGAEIFHELKKVLKKMGHVTAVGDEGGFAPNLSSNEEPLEV
                     IVQAIKNAGYVPGKDVVLAMDVAASEFYDEETKTYVLKKSGQGVKTSDEMIAWYGKMI
                     EKFPIVSIEDGLGEKDWAGWKKLTDALGHKIQLVGDDLFITNPSILKEGIEKGICNSI
                     LIKLNQIGTLTETLDAIKMAKDAHYTNVISHRSGETEDTTIADLAVAVNAGQIKTGSM
                     SRTDRIAKYNRLMRIEEKLGDKAKFLGLKAINCSKK"
BASE COUNT         3510 a         2676 c         2644 g         3766 t
ORIGIN      
        1 cttaaaatgg agaaagtcga caaagacagc tatgccatca atggtgtttt ccgggccaat
       61 aataaaacct ccgaacaatg gctgaatttg ttcggtgatt ttaatttatt aaaacaaaat
      121 ggtttccaaa gggcgcccgg ccttcctgct gaccggcttt ttttacttaa aaagccaggt
      181 aattgttaag gaaattcctc atcgcgacca ggaaacattc cgcttgtgta tgtggtcgaa
      241 actccgaccg taccggtggt gacatagtcg gtgatgtgca agccgccgct cgtgacgacg
      301 gcatagtaga cttttccgtc gatgtttgga tagtctttag tgttgtaata ggcggtgccg
      361 ctgtaagttc cgttaaaccg taaaagcgtc cttttcatca tgtagtcttc agctccgacg
      421 ttttcaagcg ccccgctttg cgtgcgatag acgtagtcga cgcttttcat cgccgatgcc
      481 tgcgcgccag aggcttcatc gctgccggcg agcattaaat agaggaagcc atttgtctcg
      541 tcattattcg tgtttgaggc tgaaatgatg aattcgtaat cgtcatcgag tgttgcctgt
      601 aaaatttcaa aatcgaagta gacttgcgat ttattgccga cctgtccgat ttgaaagcgg
      661 acttcgccaa aattagttat cgcactacct tcgcgtttaa aatagtaaac cgataattta
      721 ccgggttccc gggcgtttct ttgacgggca aagacaagcg cagagacgcc acctttaatc
      781 ggcttaaacc atatgctgtt cttcggcacc ggaaatgtcg tgccatcata gagtgtgata
      841 ttcgatgtat tcgaaaaaga caaagttgca cttgaataac tgggcggggt gataccgaaa
      901 gtcgccatct tcgtgtcatt aggaagtgtt cgattgtaga aatcaggcgt gacgtggctc
      961 gtccagtttg atgatgtcac tagcgtggtg ttaccactat cggccgtgta tgtattcggg
     1021 ctcgtcgcac ttgtgtcaac agtcactttg ctattgtact taaaaaggct tcctttaata
     1081 tcaacggaag tggccgtcac gcttccgatg aagaaagcgg tatcggtagc cgaatcggga
     1141 acggcggtaa cagtatttgc tgccttcgtc gtaaaggaag gaaaattagt atcgatgagc
     1201 aaatcgccgc cggcgagatc ttcgggcaga gaatcccatg aaacataggg accgattttg
     1261 ccgatcaagg aataactgct cataacttcg atgttcgtcg tccctaacga aatcttggcg
     1321 tttttcacgc cgatcgcctc catattgcca ttattgtagc cggcgacgat gccgaccgcg
     1381 ctgtcattga ccttattggt aacttcgaca ttttctaagt aaaatgactt gactgtaccg
     1441 atgacggcgg tcgtcgggtt aatcgataca ttgccgatgt tgccaaaaaa gccgattgaa
     1501 gctccgacgt taatactgcc gatgtttaat ggcttgactt tccaatcata tggatcggan
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tttgatgatg aatgggttat
     1681 tttgactgcc gtctccgccg gcatagtaat tgctcgtcga acggccgttg atcgccgtct
     1741 gaaaatcggt catcgtcgaa aaccaggcga cagaagtagc gactagcgaa agaaagagac
     1801 cgccggtcag gactccgatt aagatgatgt tcttgcgctt cctcatgctt gagtcctcta
     1861 cgccaccagg tagacgatgt cgtctcgata gatgacgtcg cggtcatttc ctatcctggc
     1921 attatcgatg taatccatca ccgcttgact gtattcgagg acaaaccatg tttcggtatc
     1981 gcccggattg attgcggcga ggtcaagtga agttactttg ctcggcgtcg caccaatcgt
     2041 cacaaatgaa tccgcatcag cgttgatata ggtgagggac gcactcgtcc agctctcgcc
     2101 tagtcctgac gaaggaccgc cggtcggcgt aatctgaaga gcattgctgg catagttatc
     2161 gtcagtctct cccgaaccgg tcgtccccga tgaaaaagtc gtatggtcgg tcgtcgcgga
     2221 aaaggtgacg atttcggcgg cgtcatacgt gaattgaacg tggacgacga tagcgggtga
     2281 gaaggtgcta ttcatttgcc actgcgggtc ataggtggcc agagaggtga cggtttgact
     2341 agaaaaggta atcgccgtat ttgtttcgcc ttttactaaa ttcgttactt catagcaatc
     2401 aaaaccggtg acggcaaagc cgggaatggt tacgcggacc gattccatat gaacctgacg
     2461 cgtcagattt tgcgcgaacc aagcgagcgt cgtatatgta aaagccccga tcccgaccat
     2521 ggcggcggcg agattggcat agacgagaat tgtggtttta aaacgctttg tcagtttcat
     2581 ggcaagttat gattatccgc aagtcggagt tcacttacaa acaaaatgct ttacatatgt
     2641 aaaaaatgac tcaactgcga acagcaagcc cccgaatttt ttagttgccc ctatgaattt
     2701 atctactaat agatattttc aatcaaattg tgtgatgcta ccttttttaa tactaattag
     2761 caccatcgag aaatattaca gcacctcgat tgaaacggct gaacaataca atgatactta
     2821 atatcaattc tattaaaata atgaaaacaa attttttgag atatgatgcc gattatcagt
     2881 attcgcgtaa agtgttgatt caagaatttt caatacattt atgaaatatt aaaacggact
     2941 aaatctatga aattctttag attctaatgc tttaggtgaa aacaataaaa tgaaatcaag
     3001 ttgatcctcg ttaaaatggt atttttccac caattttgaa cttagtaatt ctttatctct
     3061 atcttcttca tcaacttgat gattgctttt acagaaaact taggaaatat tagtgctgtt
     3121 cttttcatta cgcacctcta ctatgatgat caattcattg ataatttttt tatttaatca
     3181 taaaacatga ttgttacaca tgtctcaatc atttttgaat tctaaacgtc atctaaacat
     3241 ctatcaaatt ttagaaacat taatgtttaa gtagtcctaa aggaataaca taaataccat
     3301 cagttctttg ataacaaagt tcggtgtttg taagtatcat taaaaaggat ggtttcttag
     3361 ttttatgaat ttttgctagt tttataagat ttgatgctgc tttctcaact tcattctccc
     3421 ctaatttaac ctcgattcca gcccatctgc catccctcaa ctcaataatt aaatctactt
     3481 ctaatccaga tgaatcatta taatgataga cttcgccatc taacgattga gcatagactc
     3541 tgatatctct agtgcatata gattcaaaaa agaatccaaa tgtttcaaag tcttgtttta
     3601 gtttatctac cgataaaccc aaagcagcta cagcaatact tggatcgaca aattgctttt
     3661 tatctgatgt tctaatcgtg ctggcacttc taatagcagg agaccaagct ttaaggtttt
     3721 caataatgta taatctttga agtgcgtttg tataagtatc gtatgtttta tagtcaatag
     3781 aaataccttc gttttcttga tctttataaa tagttttact ggttgctaat gtagatatat
     3841 ttcttgcata actgcgtatg agtttcctaa ttttatcttt gtcttttttg gttccatcaa
     3901 cttcatctac atcttttgat ataatacttt caagtaagtc tttaggaata gcaagttgtt
     3961 cttttaatgg taattttaga ctacctggcc atccaccgcg acaaatatag tgagcgatgt
     4021 cattaatgct tgtttttgat tttgctactt gaattttctt accttcaaat aaatcagcta
     4081 atgagaccga gccaatagaa tctccgcttt caaataaact cataggacgc atgtatatag
     4141 aattaattct tcccgttcct gtatgagata tatttatgtt ttgcttcttt gtcgagcctg
     4201 taagtaaata cgcaccttta tatttgttat catctatatc aagccttata aaatcccaaa
     4261 tctcaggaac ttcttgccat tcatcaaaaa gaatcggttt ttcaccagaa agtacttcgt
     4321 cttttgaaat ggttgctaaa gtttgatatt gctttttcgt aataggattt tgtaatttaa
     4381 tgatagtttt agaataaagc tcagctgtag ttgatttacc agtccacttt ggaccttcta
     4441 taactacagc tccactgtat ttcattttat cttcaattcc tttttcaata attctcttct
     4501 tataattacc aggtatcata attattacct ccaattgtat tttacaacaa ttccaataaa
     4561 tgtaaatatt ttattccaat tattattgac gttttattcc aattaatatt gatttgttct
     4621 caacaaaaaa agtttacacg atacaatcgt gtaaataagc ctcaaatcgt gtgagcctat
     4681 tatcagtcaa atatttttac cattattttt ggttactttt caaattgaaa ttcgcttgct
     4741 atagtgtgaa aaagccctta ttaaagcaaa aaaggcctga tacaaaatgt atcaaaccta
     4801 tgcttcttat atgcgcgatg ctacctatgt taatacatat tagcacccca tcattttttt
     4861 gcacccatag atctattttg gcatcttatg aaaactgtta aatcatatgg aagctaaata
     4921 tcgaccctat aaatgctatg ctttggtata ccatcataat aaaacacaaa attattaggt
     4981 ggtgtaattg tttctatgtg gatgtcgccg agtttgttaa taacactttt cgatgggtaa
     5041 ttatcctcat cacaagaaat aaccaaatgt gtcataccat gtaaccttgc aactgagtaa
     5101 gtcatcataa cagccatttt ggcataacca tttccacgaa aaggttcgtc aatttcatag
     5161 cctacattcc cattaatata ggaatgttcg ttatatccaa tgcgaatact tatcttacca
     5221 atcatgcttg atatcgagtt caggtaaatt tcataataat aaaatggaat aactttatca
     5281 tcaccaacac aagtttctat aagttttagc gtaagtgtat cacctttaat gatcgtaaaa
     5341 ttttgattga attccataac tatacccctt ataaaaacat agtcatgtaa accggtaagt
     5401 aaataacatc atctttttgt gataaatctt ttgtatgaat aatgtatttt ttacccaatt
     5461 tgtctttaaa tttgatacta aacttgtcca atgatgaagt agtatagttt ttgccagact
     5521 taacttcaac tggtgatatt ttagttggtt tggattgatc gataattaga aaatctattt
     5581 ccatcgtatt tgaacggtct tgattatctg ctcttgagta aaaatatagt tttttatttt
     5641 cagctgtaaa catttgtgcg acgatgtttt caaatatcat accattgtta acttcaagtc
     5701 gatcttgaat aatggctttt gctacttctt gatgcatttc atcattttca ctgaatgctt
     5761 gacttaataa taaaccagtg tctgccatat acattttaag cgtcaatcga tcacgattta
     5821 aagataatcc aaaattcgga tctgatgcat taaagcatac atttgtaatc attgcttcat
     5881 ccaaccaggt aaacgcatct tcataatctc taaatcgagc atctttcgat aatgaactta
     5941 acataaactt cttttcgcgc tttgataatt gactggggat ttcatcgaaa atactgagta
     6001 ctttatgttc atatcgctta gcatactttg aaatgtcatt cttataaagc tttaagatat
     6061 ctcttttaat tcgatctact tttgtataat cattggtatt caagaattcc ataacagctt
     6121 gaggcatgcc accgattaac aagtactgtc taaaaagctt cataatcgtt gaatgaattt
     6181 catttagagg ctttaaagtt tcaaaatgct ttttaatcag agaaatgaca tcaactctac
     6241 caagtgcatt acaaaattct tcaaaatcaa gaggatacat tttgatcgtt ctttcttctg
     6301 aaggtagtaa aatactttct acattacgct tgatggataa taaagaccct gtttcgatgt
     6361 aatcataacg gttgtcatgc acgagatgtt tgatcagttg tcttgcttga gggaacaatt
     6421 gtacttcatc aaatacgatc agtgagtttc tttcatataa tgtaatccca gtgattgatg
     6481 ttaagaaaaa gaaaaaatca tcaagatttg atgcgtactt tttgaatagt gatttaatct
     6541 catcactagc aatactaaag tcaataagca tgtatgattt gtattcattt cttgcaaaag
     6601 cttcaaccac tgtgcttttt ccaacacgtc ttgcaccttc gatgagcaat gccgttttac
     6661 cgttttcatt tttccatgaa agcaattgct tatatatttt acgttccaac atcgtcataa
     6721 tcacctctta taattctatt atacacgtat tttgattttt atataaaata ttttacacgt
     6781 attttgattt ttttaaggtt tcttatatca cgttatcgca aatttacttg ttagtattaa
     6841 tatatcagcc tcgagtaaga atcttcaaaa ttatataatc gtgtaaatag tagagtaaac
     6901 gtgtatccag ttaagtaatc gtgtaactaa ataaatagga ttttcttgta tctgcgtata
     6961 gaataagatt tttgacttct acttgactat tggatgtttg aatataggga gaaaaatccc
     7021 ttacttcatg aaaaaaaggt ctaatactag ggcgaattac taattcgcat tcgtatcaga
     7081 ccaatgctcg tataatggta cggtgaatgg gactcgaacc cacaaggtgt taaccattag
     7141 aacctgaatc taacgcgtct accatttccg ccatcaccgt gcatttatta gtataaatga
     7201 tagaagcgaa tgtgcaatat tatttcttta agaaaatgac gatttttttc atgacacctt
     7261 catcttcctc ggtaatcagc tcgtaattga tttcataatc caaaacggga ttcttgcgat
     7321 catagatagc tttgtctttc tgaacttgat aaagataatc ctcggcgcgc ttgctcagat
     7381 aaggcagatg accgcgatct tcgatgtaga gcagttcgat gtttgctttg gctttggcaa
     7441 cttttgcgaa tctatcaatt gagttcttcg ctttgatgac gcgatcttcc cgaccgtgaa
     7501 ttaaaagcat cggtatcttg gccctgcgaa gagccttcac gccattgtat gtcgcatagc
     7561 gaccataacg ccagaagccg tgcatgtaga tgaagggata taaaggataa agagccgggc
     7621 ggtttgcaat cgtgacgagg ctcggagaaa gaaaagccga aagggagacg actttatcga
     7681 cgcggtgctt ttttaataag acgctgttca tggcgacata gccaccccag gaatgaccga
     7741 cgatataacg ttttaagtcc ttgatatcgc ttctcgattc gatataagaa agggcataat
     7801 caagatcaac gaggggctga atcagacttt tcatcgctct tccagagctg agggcgcagc
     7861 cggtgtaatc aaaggccaag acgagaaatc cttcttttgc caaacggttg atctcggtcg
     7921 tgtaggcggt atgtcccgct ccgatgccat gggtgaaaat aacgagttct ttaaaggttt
     7981 taaattgttg atttgaataa agataaccgc gcaaaacctg ctttttattg ccgttaaatt
     8041 taatcggttc gccgacgagg ccgacaaaat cctcggcctg caaatagcgg agggcaggtg
     8101 atccatcgcc ccgcttgtta agcgccagtt taaacatcac gttaacaaaa gcagaaaggg
     8161 cgataatcac gatgactagc gcgatgccgg caataattaa tccgcgggtt aannnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcgccct attgctcatc atgcccgaca
     8341 tcgtatatcc gcgaagcggc atcgtcagag cgctctcgat gaacatcctg cgcgtcgctt
     8401 cgatatcctc gcccgtcatc ttgttagcca tgttatgcag tgtcttggta atgattttgc
     8461 caaccatcgt atcttcgata tcttttaaag tcgagttttt gctaaagggc cgaattttac
     8521 ggtcgcgggc cacgggaatc ctgtcctgaa ggattttttc aaattgctca tcgctaactg
     8581 tcaagggatg gtgaggggcg tagtaatcgc cgaggtcatc gtcgacaagg tgactcgcgt
     8641 cttcgccttc aatcagcacc tctttgctca ggcgaagatc gcgagaggag gcgcctacct
     8701 caaagacata tcgacctttt tccagaaagt attcgtgcgt tgtcaaatca tagatacgca
     8761 ggtcatcctg atcgatatag attgtcactc tcttcgtctc gttcggttca agtcttactt
     8821 tggcaaaacc ccttaattca tgactgggct tatagacccc tgcagctttg ctggcgacgt
     8881 agagctgagc aatttcggca cctgatgtgg ccccggtatt cgtcacatca aaagagacag
     8941 cgtaacgaat tttgccctcg ctgaactcgt attttaaaac gagattagca taggtaaaag
     9001 tggtgtatga gagaccatat ccaaaaggat agcggacggc gatgttaaaa gtatcatagt
     9061 agcgatatcc gacataaatg ctctctttat ataaaacatt cgtgccatcg cccggaaaga
     9121 aattacctga aggaacatcc ttatacgtca gcggccatgt ttcgggcagt ttgccggagg
     9181 ggttgactcg acctaacaaa acgtcgacaa cagcgggtcc gcatgcctca ccggctagat
     9241 aagtcaaaag gaggccgtca acctgatcgg caaagggcag ttccatcacc gaaccaccgg
     9301 ctaagatgat gatcactttt ttctttaagg ccacgacttt ttcaattaga gagaggtgag
     9361 aatccggcaa gctcatcttg ctgcggtcaa acccctcgct ttcataggca tcgggaaggc
     9421 cggcaaaaat aacgatgata tctttttgct tagcgagttc aatagcttct tgttcgaact
     9481 tatcatcaat ttcatcgccg ttagatttat agcctaaagc ataattagct ttaatatttt
     9541 catttttcaa agcctcaata aaggacacaa gacggaaagg attcaccttc gaactacccg
     9601 tgccctgata gcgcggaagc gcggcaaagg ctcccaaaat cgctacatta tcaaaacttt
     9661 ttagtggcaa aacgccatta ttttttagca aaacgctgct ttcggcggcg attttacgcg
     9721 ctaaagagaa atgctcttcg taatggccgt cattcccgta ctcaagttcc ttaactttta
     9781 aatgaagttc ataaatatta ttgacacaaa cattaacatc atcttcatcg aggaggcctt
     9841 ttttaatgcc gcactttaaa ttgatgtctc gccttccaat gcccggcatc tcgaggtcgg
     9901 tgccgctttt acgagccagg aaatcatcgt tgatcgcccc ccagtcggtg acgactaaac
     9961 cgcgataccc ccacttgttt cgcaaaacat cattgaggag ccagttattt tcgcttgagt
    10021 aggtgccgtt aatgcggtta taagagcaca tgatggtata aggttttgct tccttcaccg
    10081 ctatttcaaa gggcttcaaa tatatttcgt gaagagcccg cgcatcgatg acggcattga
    10141 tagtcaaacg gttggtttcc tgattattcg cggcgtagtg tttaagtgag gtgccgacac
    10201 cgcgactttg aacgccttta ataaaggcgg cccccatttt tcctgagagg agcggatctt
    10261 cggaaaaata ctcaaaattc cgaccgcata gcggatttcg tttgatattt acgccgggtc
    10321 ccaagacgac ggcgacatgc tgatgaaggg cttcgcgggc gatggcttgt cccatctgaa
    10381 aaatcaaatc ttggtcaaac gagcaggcca ggagcgaagc ggtcggaaaa cacgtcgcag
    10441 gaaaggcagc cataatgcct aactgatcgc ccttgtcaac ttgctttctg aggccgtgcg
    10501 ggccatcggc gaccaagaat ggttttatgt taaagtcttc gatgccgcgg actttccaga
    10561 aaccatcacc atagagaagt tcaagcttct tttgaagagg taaggattta atctcgtcag
    10621 gtattttttg atattgagcc ataaattaat tataaacaaa aaatcactcc gaagagtgat
    10681 aattttataa aatagcggcg cttatccgat cagcacgctg agcaggagca tgacgatgaa
    10741 accgaccata aaaccgacgg tagccaaatt tgagtgcgcg ccttcctgcg actgcggaat
    10801 cagctcttcg atgacgacat agatcatcgc cccggctgcg aaagccagca aatagggaag
    10861 aatcgggacc atcagctcgg tgagaagaat ggttaagatg gcggcgacgg gttcgatgat
    10921 gccggtcaga ccgccgatta aaaatgattt tgtccgcgaa tggccttcaa gcctgagggg
    10981 caaagagacg atcgcccctt ccggaaagtt ttgaatggcg atgccgatag ctaatgccaa
    11041 agcgcccata aaagtgatgc cggcattgcc gcttaaagcc ccggcaaaag tcacgccgac
    11101 ggcaatccct tcgggaacat tatgaagggc gacggcaaac accatcatcg aggtgcggct
    11161 gatcttcttg gtcttaacgc cttcaggctt ctcactgttg atgtgaaggt gaggcgtgat
    11221 cgtgtcgagc aaaagcaaaa agcccatgcc gagagcaaag ccgatgacaa cggaaacaat
    11281 tccgccgtta accggcgctt cgagagcagg aataataagc gaccagatgg aagctgccac
    11341 catgactccg gaagcaaagc cgagcaaaac cttctgcagc cacggcttga ttccgttctt
    11401 ggcgaaaatg accatcgagg cacctaaggc ggtgccgacg aaaggcatag ataaaccgat
    11461 gatgaggatt atccaatccg cttgggtcag gggcagagac ataaattagt tatttctttg
    11521 agcagttaat ggctttaagg ccaaggaatt tagccttgtc gccgagcttt tcttcgattc
    11581 tcatcaaacg attgtatttg gcgatgcgat cggttctcga cattgaaccg gttttaatct
    11641 gaccggcgtt gacggcgacg gccaagtcgg cgatcgtcgt atcttcagtt tctccggaac
    11701 ggtgggagat gacattcgtg taatgggcgt cttttgccat tttgatagca tcgagcgtct
    11761 cggtcaaagt gccgatttgg ttgagtttaa tcaagattga gttgcaaatc cccttttcaa
    11821 ttccttcttt gaggatcgag gggttggtga tgaataaatc gtcgccgacg agctgaatct
    11881 tgtgaccgag ggcatcggtt aatttcttcc atcccgccca gtccttttcg cctaagccat
    11941 cttcgatgga gacgatgggg aacttctcga tcatcttgcc ataccaggca atcatctcat
    12001 cggaagtctt aacgccctga ccgctctttt taagaacata ggtcttggtt tcttcatcgt
    12061 agaattcact ggcggcgacg tccatcgcta agacgacatc tttgcctggg acatagccgg
    12121 cgtttttaat ggcttgaacg ataacttcta aaggttcttc gttagatgag aggttcggag
    12181 caaagccgcc ttcatcgccg acggcggtga catggcccat cttcttgaga acttttttca
    12241 gttcgtggaa gatttccgca ccccagcgaa tggcttcgcg gacgctgggc gcgccgacgg
    12301 gcatgatcat gaattcctga aagtcgacgg aactatcagc gtgcgacccg ccattgatga
    12361 cattcatcat cggaacggga agggtgacgc cgacttcgcc ggcgttaagg gcgatttgtt
    12421 catagagcgg gacatgattg aaatccgccg cgcatttgac ggcggccagg gacacgccga
    12481 ggagggcatt cgcgccgaga ttagatttaa atttggtgcc atcgagggcg agcaatttgg
    12541 cgtcgatgac tttttgttct ctgacatcca tgccgaccat ggcgggagcg agaatcttat
    12601 taacattttc aacggccttt aaaacgcctt tgccgccgaa gcgcttctta tcgccatcgc
    12661 gtaattcgag ggcttcgcgc tcgccggtgg aggcgccgct ggggacgatg gcgcggccga
    12721 acgccccgca gtaagtttga acttcgactt cgatcgtcgg attgccgcgt gagtcgatga
    12781 cttcgcgggc aaagac
//
LOCUS       DAOSSL010000025        12515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5053_length_12515_cov_17.427769, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000025 DAOSSL010000000
VERSION     DAOSSL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5053_length_12515_cov_17.427769"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..991)
                     /locus_tag="PLH17_03390"
     CDS             complement(38..991)
                     /locus_tag="PLH17_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOR20847.1"
                     /translation="MNKDKPSVQVIKLKPVHLEKIWGGTWLKEEFKFKTKSKNIGESW
                     LISAHPGGDCRIANSVYKGKTLSELYSLRRDLFADDPHEKFPLLVKLIDAREDLSVQV
                     HPNDDYALHNENDVGKSEAWIILNAQPQTRIQIGHRARSREELEKLVKAGEWNKLLAY
                     RKCSKGEIINIPSGTIHAICAGTALIEIQQSSDLTYRLFDYDRVDKDGRKRDLHLQKS
                     LAVIDVPHRFAKRIFMPRRKDYEALAPILKTPYFRIGSMRVFDSLFFYNLQHKYYLIA
                     VLEGRGKIGKLSAHKGDSFILTSDAVKTKFQGQLNLIISTL"
     gene            complement(988..2688)
                     /locus_tag="PLH17_03395"
     CDS             complement(988..2688)
                     /locus_tag="PLH17_03395"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOR20848.1"
                     /translation="MLTDIAIWRAQQNLEPKLKDELSRLDEKGLFEAFGDDVSFGTGG
                     IRGIMGVGTNRLNIYTIRKASLGFANYIKENLTARPYSLHNVVVAYDTRNNSRLFAEE
                     TARVLASQNIHVWLFEQFRPTPELSFAVRYLRADGGIVITASHNPPEYNGYKVYDENG
                     CQLVPHKADKVGAAIEKIKDIFALKVDSLQDYLARGAITTLEKYFDNKYLTAVRFVSC
                     QKVDQDELKIVYTPLHGTGSAFGAQLLRENGYNVYSVDAQMVPDGNFSTLKSPNPEDP
                     HAFALAVELGHKVGAQLLLATDPDADRVGSGILIGDKYHFLNGNEMAAIMFDYLIKYG
                     VRIRQPVFISTIVTSDLVFEMAKRNNIETIRTLTGFKFICEQIEYIKQDRKDFFFGCE
                     ESYGYLISDLVRDKDAFQACLMIAEITAFYQNRDKNLLMVLNDIYQEYGFYKEKLLNI
                     KSPGIDGIKNIAAFMDEMRRAKFNKFGGEKILVTEDYFSSRATYCDGRVDNINLPSSD
                     VLKYVFENGWIVFRPSGTEPKLKVYIAVHSDSDAGAKDLLEKTQSAVEKLLSEKGFLQ
                     "
     gene            complement(2865..4772)
                     /locus_tag="PLH17_03400"
     CDS             complement(2865..4772)
                     /locus_tag="PLH17_03400"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOR20849.1"
                     /translation="MKTRSKLLSLLFLSFVPLALLSGCDGPSTDGLLYNTKIHAVGSK
                     AKMSSLLKEAEQENAKAYSGSGFFNTDDGPVAEDSGSGSRDYVDTNEQVEGVREGDIV
                     KTDGYDIYYAPRYHNVIRVVAVEDDYDVTLARTIELGNTYVESLYLLDKYLVVIGYTY
                     EEDLGYGDEEVMYYRWWSPTGTVMVIDRSTFDVVYQLITDSFFMDHRMIENSVFLVSH
                     KYLYYGETEYEYRPIYKETRDGITEVEYLDYDSMYYFDDSPTYGMNVLTGIKITDEPD
                     DIFYTSNAYLGASASYKKMYVSATDMYLVESVYHYDDNSYWTTSVISQYVLDIDNANS
                     SYVAAAIVAGISLNQFSIDVYDGFLRVATTEREASWAFLEFFGWSSENKITNHLFILK
                     VVAESESFELIGHMSEGLGKPNEEIKSVRFEGETAYIVTFLLTDPLYIIDLSKPSEPT
                     IAGQIVQEGFDTYQHEWGTGNLIGIGYDADQFGSITGMKISAYNVTSGEEETLQTFAI
                     FSYGYSDNSSWSYGYSEALYNHKSLLLSVEKGYLGFAVEAMEYGYTDGAERDWYYHYH
                     SYYYLFKIDFSKENPIADPIIIQHPTSDDYYLGVDRGVMIDDYVYTLSDYGVITYSLA
                     ADAIVEPPLIF"
     gene            complement(4856..6004)
                     /locus_tag="PLH17_03405"
     CDS             complement(4856..6004)
                     /locus_tag="PLH17_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOR20850.1"
                     /translation="MKIASATKRIRVKGMFSSCVLVLLVVGCAQNLEGKFDSLTIIEG
                     YKNIILMIGDGMGENHIKVTEAYNQKETAITDKAIIKGYMTTASSSSSVTDSAASATA
                     MSTGQKVPNKAIAYKNGTKLTTMSEFAKAYQKGVGIIATETVTGATPAAFSSHNRQRS
                     NTDEIALEQFQSDIDLFIGAGKSYFDQQVGLMSQNQLSYYSDFLTFQDDLDSASISHS
                     NFASRFLAAFDTISTTITTPQSPTLSDIVDVSLNYLSSRYAEEGFFALIEGSHIDKRA
                     HDGDIFGMIEQLNGFDRAVGEAVDWAREHGDTFVMVTADHETGGLKYDGEVKSEINDG
                     MFSSGGHTSVNVPFYLYNNDNLSFISADDVIDNTDVAKLLRAMIRAQK"
     gene            6085..6675
                     /gene="rdgB"
                     /locus_tag="PLH17_03410"
     CDS             6085..6675
                     /gene="rdgB"
                     /locus_tag="PLH17_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOR20851.1"
                     /translation="MKIVLATNNSHKIKEYRSLFANMPIEVLSLSDVAIKSDIEETGT
                     SYEENAAIKAENIAQLTKFLVIADDSGLEISALGGSPGLNSARFAAQHKSQSEANRAI
                     LKSLEKSDNRQARFVCVIALANYQRRLNLFRGECLGRILMRPEGESGFGYDPIFFSDE
                     ANACFAAIGEDEKNRYSHRAKAVEQLLAYLADEKFI"
     gene            complement(6668..7558)
                     /locus_tag="PLH17_03415"
     CDS             complement(6668..7558)
                     /locus_tag="PLH17_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20852.1"
                     /translation="MRKGVCPNCQKPVDYDEGQTVQTCPSCQKDYLTSQASKLYGLLY
                     SQHASNGNIALNTSMNFDKALGEYEKLLALDEESIDAVFGIALAKISLAKLGEDIIPE
                     LIAFLDGKITKIIAKGNYLPEIARYLLTLGFRYDRYFERASEVLKINNQYLDEAAKAR
                     FQVVLKQAITLWEFIIANLEKTKSSDDLSSAQERLTSLREADSGIDAYQVISKGAVKY
                     EEIKENAVFENRVALFKVRIVFMIAQMVFVVGAIIGFSIMMSNYATNPIPGLIVFGVF
                     AVLFLISNIGGRIVKNKLSK"
     gene            7715..9085
                     /locus_tag="PLH17_03420"
     CDS             7715..9085
                     /locus_tag="PLH17_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOR20853.1"
                     /translation="MANKVDLLNEKIYPSLLRFVTPLLLMWLQTSVFGLVDLLIISNY
                     TDTFAIDAVAGGISVMNIITYTIAALTTGGGILIGQYLGAKKHEQVKKLIGNMFVLFG
                     VIGIVIAAIMASLSGVIVSWLNLEGQARVDGFNYLIITAIGIPFIMGFNIIASQLRAF
                     GNSRSPFLFLVLAASVNIALDLVFVGLLEMRATGAALASIISQVCSFSAALIYLRVRN
                     FPIPLSKSDIRFSGKCSKMTLRTGLPLALQDGVVLLSFTVIMALINLYGPEAGSGFGI
                     GEKITFFCFVPLNAFSAALTATTAQNLGANQLERTKQYAKAGLLMHGVLVSVAVIICE
                     IFAPQLAALFTSDASIINYATSFIRIIAIDAFICIFIYVLNALFMGSGHTTFSMMQNV
                     LSTVLIRLPLALVFVFALETQVTVLAFAYPIASIFSLIACLIYFKMGRWKIIRQEKVH
                     IPDILS"
     gene            complement(9091..9723)
                     /locus_tag="PLH17_03425"
     CDS             complement(9091..9723)
                     /locus_tag="PLH17_03425"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOR20854.1"
                     /translation="MIYQEVLKRINAVQKLRTKDIDDIAKDYLTRGVDVDDLFPHIDE
                     NGALFRIYLVVSLKRIQKYEDQIAFIEDLFPHLRDWWHVDILPQLLKRAPSFDYVYGL
                     SAKYVQSDLLFVRRWGYVIFLTGFQKDPSLTKNILNLMHNDAAYYVQMAEAWLIADLA
                     IYNPEEILRFIASRKLNYGIIGKAIQKMCDSFRISDEIKRRARELRALYK"
     gene            complement(9785..10621)
                     /locus_tag="PLH17_03430"
     CDS             complement(9785..10621)
                     /locus_tag="PLH17_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20855.1"
                     /translation="MKLFGLVVFSFLIAGTAGAKIDTNRSALLLEDPPALSDYLDNFG
                     HAYTRVTAINWTSDAYNAIVTSGLNTYPFTSTRTTLFIENGLYMYDDVEAVNSGYYTK
                     NSESDVMYHFALEAGKDARNNADAAFSDDSYSGGVDVDEYYVGMHTINSNLDTFTALF
                     THESTNEGVITWSSTSLSETAMTLKNLGNVDEAYTLEKAMMYLTAPCYTNPDIGEDEQ
                     PYFTFNEVKIIYNSAGELVFKLYANETAQLNEGSGLFATATISNPGTTIVALDSHINP
                     SV"
     gene            10988..11494
                     /locus_tag="PLH17_03435"
     CDS             10988..11494
                     /locus_tag="PLH17_03435"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HOR20856.1"
                     /translation="MVPRSSYTKKRLVESLLKLMKKGKRFDDISIQDIVEEGGFSRMA
                     YYRNFKTKADILRYHFDVITEDFIKETNTDFYKMPFEEYLTSVFTYLFNNRELSKILL
                     ETRLFDYVKEQFDLKLIKTAHTEEERIRFIYFAGGLFNIYYHWLLSDGKQTPQEIAKI
                     VAAYVPRP"
     gene            complement(11536..>12515)
                     /locus_tag="PLH17_03440"
     CDS             complement(11536..>12515)
                     /locus_tag="PLH17_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20857.1"
                     /translation="FIDGKCFFPREDNNERLIILMIKSKVFVLTTCFVFQKSDKETFK
                     EIQKQLASLRDLPLGTDEEKKNKIRRMMEIVSAFKAAYITIDQNDDVNATHEKIITHE
                     IKHYAEDNTLLILKRKPVEAEAIVEPKKIIEEEISIDEEDVCQIDLSSGSIKRVEEKP
                     LTKIMKDKTLLLKVFKVNRISYLIAAVAILFSILFIAIAPHYFVTGDIFMGVFLIISC
                     PLFLYIAYMIVLSGFDFMDNPLKNTKTRRLITLVYAEIIAFLSILLAVGAFFLLGMNN
                     FLIDLATYHFVYSIGAIAIAVIHLAIPFFAEPLRKFNKLIKNLLKKNTK"
BASE COUNT         3426 a         2741 c         2705 g         3643 t
ORIGIN      
        1 ataaaaataa tatttactcg ccaataaaat aaactaatta aagtgtcgaa ataatgagat
       61 taagttgtcc ttggaatttc gttttaaccg cgtcggaagt caggatgaaa ctatcgcctt
      121 tatgagcgga taatttcccg atttttccgc ggccttcaag gacggctatc agataatatt
      181 tatgctgcaa attatagaag aataacgaat cgaaaactcg cattgaaccg attcgaaaat
      241 aaggggtttt taatatcggt gccagcgctt cataatcttt tcttctcggc ataaagatgc
      301 gttttgcaaa gcggtgcggc acatcgatca ccgcaagcga tttttgaagg tggagatcgc
      361 gtttacggcc gtctttatcg acgcgatcgt aatcaaataa acggtaagtg agatcggaag
      421 attgctgaat ttcgatgagc gccgtgccgg cgcagatggc gtgaatcgtt ccgctaggga
      481 tgttgatgat ctcgcctttt gagcatttgc ggtaagctag cagtttattc cattctcccg
      541 ctttaacgag tttttcgagt tcttcgcggc tcctagcacg atggccgatt tgaattcttg
      601 tttgcggctg agcatttaaa attatccatg cttcggattt tccgacatca ttctcattat
      661 gcaaagcata atcatcattg ggatgcactt gaacgctcaa atcctcgcga gcgtcgatta
      721 acttgacgag caggggaaat ttttcgtgag ggtcatcggc gaataaatcg cgccgcaaag
      781 aatataactc actcagagtc tttcctttat aaacgctatt cgcaattcgg caatcgccgc
      841 cgggatgcgc cgaaataagc cagctttcgc cgatattttt gctttttgtc ttgaatttga
      901 attcttcttt aagccatgtg ccgccccata ttttctcgag gtgaacgggc ttaagcttaa
      961 tcacttgcac tgatggtttg tctttattca ttgtaaaaat cctttttcgc ttaagagttt
     1021 ttcgacggcg gactgagtct tttcaagcaa gtctttggcg ccggcatcac tatcgctatg
     1081 gaccgcgata taaactttta atttcggctc cgtaccagat ggccgaaaga cgatccagcc
     1141 attttcaaag acgtatttta aaacatctga cgagggtaga ttgatattgt ctacacggcc
     1201 gtcacaatat gtggctcgag atgaaaaata gtcttcggta acgagaattt tctctccgcc
     1261 aaacttattg aacttggcac ggcgcatctc gtccataaat gctgcgatgt tttttattcc
     1321 gtcaataccc ggagatttaa tgttcaaaag tttttcttta taaaatccgt actcttgata
     1381 gatgtcgttg agcaccatca gcaaattctt atcgcggttt tgataaaaag cggtgatttc
     1441 agcaatcatc aggcaagctt gaaaggcgtc cttatcacgg accagatcgc tgattagata
     1501 accatacgac tcttcgcagc cgaagaagaa atctttgcgg tcctgcttga tgtattcaat
     1561 ctgttcgcaa ataaatttga atccggttag ggttctgatt gtctcaatgt tatttctctt
     1621 ggccatctcg aagactaagt cactcgtcac gatcgtcgag ataaagacag gctggcgaat
     1681 gcgaacaccg tatttgatta agtaatcaaa catgatagcc gccatttcgt tgccattcaa
     1741 aaaatgatat ttgtcgccga ttaagattcc gcttccgacg cgatcggcat caggatcggt
     1801 ggctaagaga agttgcgcgc caactttgtg gccgagttcg acggcaagag caaaagcgtg
     1861 cggatcttca ggattgggtg acttaagggt tgaaaaatta ccatcaggaa ccatctgtgc
     1921 atcgacggag tagacgttat atccattttc gcgaaggagc tgggcgccaa aggcggaacc
     1981 ggtaccgtgc agcggagtat agacgatttt aagttcgtct tgatcgactt tctgacacga
     2041 gacaaagcgg acggcagtca aatatttatt atcgaagtat ttttccaaag ttgtgatagc
     2101 gccgcgagca agatagtcct ggagagaatc gacttttaac gcaaaaatat ctttaatttt
     2161 ttcgatggcc gccccgactt tatcggcttt atgcggcacg agctgacaac cattttcgtc
     2221 ataaactttg tagccgttat attccggcgg attatggctg gcggtaatga cgatgccgcc
     2281 atcggctctg agatagcgaa cggcaaagga caattcaggg gtcggacgga attgttcaaa
     2341 cagccagaca tgaatatttt ggctggcgag aacacgcgcc gtctcctcgg cgaataatcg
     2401 cgagttattc cgcgtgtcat aagccacgac aacattgtgc aaactatagg gtcgggccgt
     2461 cagattttct ttaatgtaat tggcaaaccc cagtgaggct ttgcggattg tgtagatatt
     2521 taggcgattc gtgccgacgc ccatgatgcc gcgaatgccg cccgtgccaa aggagacatc
     2581 gtcgccaaaa gcttcaaaga gtcctttttc gtctaaacgg ctgagttcgt ctttgagctt
     2641 cggttcaaga ttttgctgtg ctctccaaat cgcgatatcc gttaacatgg ttccttttgc
     2701 acctcgctat cattaatcga atccatctac caaaagtata gatgtttacc ttgattacat
     2761 caataaaaca gaagaaatca tgttctttta tgagtaaaca aaagcatgtt gacgaataaa
     2821 agaaaagcgg gattacccgc ttgttcgtta ttattgctta ttagttagaa gataagaggc
     2881 ggttcgacta tcgcgtcagc cgcgagtgaa tatgtgataa ctccgtagtc tgaaagagta
     2941 taaacataat catcgatcat cacgccacga tcgacgccga gataatagtc atcggaagtc
     3001 ggatgctgga tgataatcgg atcagcgatc gggttctctt ttgagaaatc gattttaaag
     3061 agatagtagt agctgtggta atgatagtac caatcccttt cggcgccatc ggtatagccg
     3121 tattccatgg cttcgaccgc aaaaccgagg taaccttttt ccacgctgag caatagagat
     3181 ttatgattat aaagagcttc tgaatagcca tatgaccagc ttgaattatc cgagtaacca
     3241 tatgagaaga ttgcaaatgt ttgcagcgtc tcttcttcgc cggaagtgac gttgtaggcg
     3301 ctgattttca tgccggtaat tgagccgaat tgatcggcgt cataaccgat accaatcagg
     3361 tttccagttc cccactcatg ctgataagtg tcaaaacctt cctgaacaat ctgaccggcg
     3421 attgtcggct cggacggctt tgataggtca ataatataaa gaggatcggt aagcaagaaa
     3481 gtgacgatat atgcggtctc gccttcaaag cgaaccgatt tgatttcttc attcggtttg
     3541 ccgaggcctt cactcatatg accgatgagt tcaaatgatt cgctttcggc gacgacttta
     3601 agaataaaga gatgattggt aatcttgttc tcgctcgacc agccgaagaa ttctaagaac
     3661 gcccagctcg cttcccgctc cgtggtcgcc acccttagaa agccatcata gacatcaatt
     3721 gaaaattgat taagcgaaat accggccacg attgccgccg cgacataaga gctgttagca
     3781 ttatcgatat cgaggacata ctggctgatg accgaggttg tccaatacga attatcatca
     3841 tagtgataaa ccgattcgac aaggtacatg tcggtcgcgc tcacatacat ctttttatag
     3901 ctggcggagg cgcctaagta agcattggaa gtatagaaaa tatcgtctgg ttcgtcggta
     3961 atttttatcc cggtaagaac attcattccg taagtcggag aatcgtcaaa ataatacatt
     4021 gaatcataat cgagatattc gacttcggtg atgccgtctc tcgtctcttt ataaatcggc
     4081 cggtattcgt attcggtttc gccgtagtac aaatacttgt ggctgacgag aaagacgctg
     4141 ttttcaatca tgcggtgatc catgaaaaaa ctatcggtaa tcagctgata gacgacatca
     4201 aaagtcgacc ggtcgatgac cataaccgtt cctgtcggcg accaccagcg ataatacatc
     4261 acttcttcat caccatatcc taaatcttct tcataggtat aacctatgac aactagatat
     4321 ttatcaagta aataaagcga ttcgacataa gtgttgccca gttcaattgt ccgcgctagg
     4381 gtaacatcat aatcatcttc gaccgctacg acgcggatga cgttatgata gcgtggcgca
     4441 taatagatat cgtagccatc ggtcttgaca atatcgcctt cgcgaacgcc ttcgacttgt
     4501 tcgttggtgt cgacataatc gcgagagccg gaaccgctat cttcagcgac gggcccgtcg
     4561 tctgtattaa aaaaaccgga gccggaatac gctttggcat tttcttgctc agcctctttt
     4621 aaaagcgaac tcatttttgc cttggagccg accgcatgta tctttgtatt atacaaaagg
     4681 ccatctgtcg aaggtccatc gcatcctgaa agaagggcaa gcggcacaaa agagagaaac
     4741 aacaagctca acaatttact gcgcgttttc ataattcatc tccttgctaa taaatcgctc
     4801 gacacctata tagacaagtg atctgacgtt ttttttatga aatttctttt atttattatt
     4861 tctgggcgcg aatcatggcg cgcaagagct tggcgacatc ggtgttatca ataacatcat
     4921 cggcggatat gaaggacaaa ttatcattat tgtatagata gaagggaaca tttacggatg
     4981 tgtgtccgcc gcttgaaaac atcccatcat taatttcact tttaacttcg ccgtcgtatt
     5041 taagaccacc cgtttcgtga tcggccgtca ccatcacaaa agtgtcgccg tgctcccttg
     5101 cccagtccac cgcttcaccg accgcccgat caaacccgtt tagctgctcg atcatgccaa
     5161 aaatatcgcc gtcatgcgct cttttatcga tgtgacttcc ctcgattaaa gcaaaaaaac
     5221 cttcttccgc atatcgactt gagagatagt ttaaggagac atcaacaata tcggataaag
     5281 tcggtgattg aggtgtggta atcgtcgtcg aaattgtatc aaaagctgcc aagaacctcg
     5341 aagcaaagtt gctatgtgaa atactcgctg aatcgagatc atcttgaaag gtgagaaagt
     5401 cggaatagta actaagctga ttttgactca tcaaaccgac ctgctgatca aaataagatt
     5461 tcccggcgcc gataaataaa tcgatatcgc tttgaaattg ttcaagggcg atttcgtcgg
     5521 tgttactccg ctggcgattg tggcttgaaa aggctgccgg tgtcgctccc gtcacggtct
     5581 cggtcgcgat gatgccgacg cctttttgat atgctttggc aaattcggac atcgtcgtga
     5641 gtttcgttcc gtttttataa gcgatggctt tattcggtac tttctgacct gttgacatgg
     5701 cagttgccga agcggcgctg tcggtgactg aagacgatga ggaagcggtc gtcatatagc
     5761 ctttaatgat ggctttgtcc gttatcgccg tttccttctg attataagcc tcggtgactt
     5821 tgatgtggtt ttcgcccatt ccgtcgccaa tcatcaaaat aatattttta tagccttcga
     5881 taatcgttaa actatcaaac ttgccttcga gattctgagc gcagccaact acaagcaaaa
     5941 cgagaacgca cgaactgaac attcccttga cgcgaattcg tttcgttgcc gatgcgattt
     6001 tcataaaatt atcctatcac aagtggccga ttacatttca caagcattcg cccactcagt
     6061 aaattatatg ctatatttaa aggcatgaaa atcgtccttg ccaccaataa ttcacataag
     6121 atcaaagagt atcgctccct atttgcaaat atgccaattg aggttctctc attaagtgat
     6181 gtcgcgataa aaagcgatat cgaggagacc ggcacatctt atgaagaaaa cgcggcgatc
     6241 aaagcggaga atattgcaca actgactaaa tttttagtca ttgctgacga ttccggtttg
     6301 gaaataagcg cccttggagg gtctccgggt cttaattcgg cgcgctttgc ggcccagcat
     6361 aaaagccaat cagaagccaa tcgggcaata ttaaaaagcc ttgaaaaatc cgataatcgt
     6421 caggccagat tcgtctgcgt aattgccctc gcaaattatc aacgaaggtt gaacttattt
     6481 cgcggagagt gtttagggcg aattcttatg cgcccagaag gagagagcgg ttttggctac
     6541 gacccaatct tcttttccga tgaagccaat gcctgtttcg ccgcgatcgg cgaggatgaa
     6601 aaaaaccgct attcgcatcg tgcgaaagcg gttgaacaat tgctcgctta tttagcggat
     6661 gaaaaattta tttagacaac ttatttttta caatccggcc accgatatta gaaattaaaa
     6721 acaagacggc gaagacgcca aatacaatca atccgggaat cgggtttgtc gcatagtttg
     6781 acatcattat tgaaaagccg atgatcgccc cgaccacaaa taccatctgc gcaatcataa
     6841 aaacaatgcg cactttaaaa agcgcgacgc gattttcaaa gacggcattt tctttgattt
     6901 cttcatattt aaccgcgcct ttagagatga cttgataagc gtcaattccg ctatctgcct
     6961 ctctcagact agtaagtctt tcctgagcac ttgataaatc atcagaagat ttcgtctttt
     7021 caagattagc aataataaat tcccatagag ttatggcttg ttttaaaacg acctgaaaac
     7081 gtgccttcgc ggcttcatca agatactgat tatttatctt caaaacttcc gatgcgcgct
     7141 cgaaatagcg atcgtaacga aatcctaaag tcagtaaata ccgtgcaatt tccggtaaat
     7201 aattgccttt tgcaatgatt ttagtaattt ttccatcgag aaaagcgatg agctcgggaa
     7261 tgatgtcttc acctaatttt gccaggctga tcttggcgag agcaataccg aaaaccgcat
     7321 cgatgctctc ctcatcaagg gcgagaagct tttcatattc tcctaaggct ttatcaaaat
     7381 tcatcgatgt gtttaaagcg atgttgccgt ttgaagcgtg ttgcgaatac aaaaggccat
     7441 atagtttgct ggcctggcta gttagataat ctttctggca gcttggacaa gtttgaactg
     7501 tctgaccttc atcataatcg actggcttct gacagtttgg acacacacct tttctcatat
     7561 ctctcctcaa ttcattacat actggattgt tacatacaac attgccgata tgacaatttg
     7621 aacataagtt ttactaaagt acacttgctg ataaatatca tcgctaattt tatcatacct
     7681 tcagtttgct tatgatatac tttttgcgac ccctatggct aacaaagtcg atttgcttaa
     7741 cgaaaaaatc tatccctcct tgctccgctt tgtgacgcct cttcttctaa tgtggcttca
     7801 gacctcggtg tttgggcttg tcgacttgct catcatttca aattacactg acactttcgc
     7861 catcgatgcc gtcgccggag gcatatcggt gatgaatatc ataacataca cgattgcggc
     7921 cctaacgaca gggggcggca ttttaatcgg acaatacctc ggggccaaaa agcacgagca
     7981 agtcaaaaaa cttatcggca atatgtttgt cttatttgga gttatcggaa tcgttatcgc
     8041 cgccatcatg gcctccttgt cgggcgttat cgtgtcgtgg cttaatctcg agggtcaggc
     8101 ccgagttgat gggtttaatt atttgataat tacggctatc gggattcctt ttatcatggg
     8161 ttttaacatc atcgcctcgc agcttcgcgc ttttggaaat tcgcgttcgc catttctttt
     8221 tctcgtcctg gccgcttccg tcaatattgc tcttgactta gtgtttgtcg gcttgcttga
     8281 gatgcgggcg acgggagcgg ctctagcttc catcatctcg caggtatgct catttagcgc
     8341 agcgcttatc tatcttcgcg ttcgaaactt cccgattcca ctatcaaaaa gcgacattcg
     8401 ctttagcgga aagtgctcca aaatgactct gcgcaccggt cttcccctcg ctcttcaaga
     8461 tggcgtcgtc ctcctgtctt ttactgttat catggctctt attaatctct atggaccaga
     8521 ggcgggttca ggatttggaa tcggtgaaaa aatcaccttc ttctgttttg ttccattaaa
     8581 cgcttttagc gccgcgctga cggcgacgac ggcccaaaac ttaggcgcca atcaacttga
     8641 gcgcactaaa caatatgcca aagccggcct gctcatgcat ggcgtactcg tgagcgttgc
     8701 tgtcatcatc tgcgaaatct ttgcccctca gttagccgcc ttgtttactt ctgacgccag
     8761 tattattaac tacgccactt cctttatccg catcattgcc atcgatgcct ttatctgcat
     8821 ttttatctat gttctaaacg ccttgttcat gggatcggga catacgacct tttccatgat
     8881 gcaaaacgtt ctttcgacgg tcttaatccg cctccctttg gctctcgttt tcgtctttgc
     8941 cctggaaacg caagtcacgg tcttggcgtt tgcctacccg atcgccagca tattttcttt
     9001 aatcgcctgt cttatctact ttaaaatggg gcgctggaaa ataatccggc aagaaaaagt
     9061 ccacattccg gatattcttt cttgataatg ctatttatat aaggcgcgca gctctctggc
     9121 gcgtcttttt atttcgtcgc taattcgaaa agagtcgcac atcttttgaa tggcttttcc
     9181 gatgattccg taattcagtt ttcttgaggc gataaagcgc aatatttcct cagggttata
     9241 gatagctaaa tcggcgatta gccaggcttc ggccatctgg acataatagg ctgcatcatt
     9301 atgcatgaga ttaagtatgt tctttgtaag ggaggggtct ttttgaaagc cggtcaaaaa
     9361 gatgacgtat ccccaacgtc gcacaaacag caaatcggat tgcacatatt ttgcggataa
     9421 accataaaca tagtcaaaag acggagccct tttcagcagt tgcggcaaaa tatcgacatg
     9481 ccaccaatcg cgaaggtgcg gaaacaaatc ctcgataaaa gcaatctgat cttcatattt
     9541 ttgaatgcgt ttgagcgata ccactagata gatgcgaaaa agagcgccgt tttcatctat
     9601 gtgaggaaaa aggtcatcga catcaacgcc gcgagttaga taatctttgg cgatatcatc
     9661 gatgtctttg gtgcgcagct tctgaactgc attaatgcgt ttcaatacct cttgataaat
     9721 catgcctgcc ccacgtctta taaaaaagcc gccccataaa agacggctca tcagtgaaat
     9781 cgttttaaac ggatggatta atgtggctgt caagagccac gatggtcgtg cccggatttg
     9841 aaatcgtcgc cgttgcgaat aagccgctgc cctcgtttag ctgagcggtc tcgttggcgt
     9901 acagcttgaa gacgagttct ccggccgaat tgtatatgat ttttacttcg ttaaaggtga
     9961 aataaggttg ttcgtcctcc ccgatatcgg gattcgtata acagggggcc gtcaaataca
    10021 tcatcgcttt ttccagagta taagcctcat cgacattacc aagatttttt aatgtcatcg
    10081 ccgtctctga aaggcttgtc gaagaccaag taatgacgcc ttcattagtg ctttcgtgag
    10141 taaataaagc cgtgaaagtg tcgaggttgc tgttgatggt atgcattccg acatagtatt
    10201 catcgacatc aactccaccg ctataactat cgtcagaaaa agcggcatcg gcattgtttc
    10261 gtgcgtcttt accggcttct agcgcaaagt gatacatgac atcggattca gaattttttg
    10321 tgtaataacc gctgttgacg gcctcaacat catcatacat ataaagtccg ttttcgataa
    10381 acagggttgt ccgtgtcgaa gtaaaaggat aggtattgag tccgctggtg acgatggcat
    10441 tataagcatc ggaagtccag ttgatagccg tcacgcgagt gtaagcgtgg ccaaaattgt
    10501 ctaaatagtc gcttaatgca ggaggatcct ccaaaagaag ggcagaacga ttcgtgtcaa
    10561 ttttggcgcc tgccgtgccc gcgattaaga aagagaagac gactaaaccg aataatttca
    10621 taataagtcc tcaactaaat atagtttcat tgtacctctt tttgttatca ggagacaaac
    10681 gatgcttttt cattcagcgc taatttaagg agagaaattc ccataagaag cagtaatgtt
    10741 tttgtttttc attttcagtt aactaatttt acggtgagaa taaataagca gaaatttcga
    10801 tatagtgaga ttaaatcgaa gaaagattgg tcttattgtt ttcaatatct actcataaat
    10861 tcagcctttt gattaaatat tttattaatt ggtgcattac aattgcaaca gtgcaagtta
    10921 ttatataagg tgttatttga taaccgataa actatactta aaagtgataa agaaagggtt
    10981 ttataacatg gtacctcgca gcagttacac aaaaaaacga ctcgttgaat cgctcctcaa
    11041 attgatgaag aaagggaaaa ggtttgatga tatttcaatt caggatatcg tcgaagaggg
    11101 tggcttttct cgcatggctt actaccggaa ctttaagacc aaagccgaca tacttagata
    11161 tcatttcgat gtgataaccg aggatttcat caaagagaca aataccgatt tctacaagat
    11221 gccctttgaa gaatatctga cttcggtttt tacctatctt tttaataatc gcgagctttc
    11281 gaaaatactg ctcgaaacgc gtctttttga ctatgtcaag gaacaatttg atttaaaact
    11341 tatcaaaacc gcgcacaccg aagaagagcg aattcgcttt atttacttcg ccggcgggct
    11401 gtttaatatc tattaccact ggcttctatc tgacggcaaa cagactccgc aagaaattgc
    11461 caaaatagtc gccgcttatg ttccgcggcc ctaaaaaatt aaagcgaaaa gttcctcgaa
    11521 atgcggggaa tttttttatt tcgtattctt tttaagcaag ttcttaatga gcttattaaa
    11581 tttgcgaagt ggctcagcga aaaatggaat ggcgagatgg atgacggcaa tggcgatcgc
    11641 accgatggaa tagacgaaat gataggtggc caaatcgatc aaaaagttat tcatgccaag
    11701 caagaaaaag gcaccgacag ccaagagaat cgataggaac gcgataatct cggcatacac
    11761 caaggtgatt agcctcctcg tcttcgtgtt tttcaatgga ttgtccatga aatcaaaacc
    11821 agataagaca atcatgtagg caatgtatag aaaaagtggg caggagataa ttaaaaatac
    11881 gcccataaaa atgtccccgg tcacgaaata atggggagcg atggcgatga aaagaatgct
    11941 gaataagatg gcgaccgccg cgattaaata agaaatgcgg ttgaccttga agactttgag
    12001 gagaagcgtt ttgtccttca tgattttcgt aagcggtttt tcttcaactc gtttgattga
    12061 tccgctgctc aagtcgattt gacagacatc ctcttcgtca atcgatattt cttcttcaat
    12121 tatctttttg ggttctacga ttgcttcggc ctcaacgggt tttcttttta aaatgagaag
    12181 ggtgttatct tccgcataat gcttgatttc atgagtgata attttttcgt gagtggcgtt
    12241 gacatcatca ttttggtcga ttgtgatgta ggccgcctta aaggcggaga cgatttccat
    12301 catccgtctg attttgttct ttttttcttc gtcggttccc aagggcaaat cccttagcga
    12361 agcgagttgt ttctgtatct ccttaaatgt ttccttgtcg ctcttttgaa aaacaaagca
    12421 ggtcgtcagg acaaaaactt tgctcttaat cattaatatg attagtcgtt cattattgtc
    12481 ctcgcggggg aagaagcatt tgccgtcgat gaaaa
//
LOCUS       DAOSSL010000026        12242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5191_length_12242_cov_13.701321, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000026 DAOSSL010000000
VERSION     DAOSSL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5191_length_12242_cov_13.701321"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..178)
                     /locus_tag="PLH17_03445"
     tRNA            complement(105..178)
                     /locus_tag="PLH17_03445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:tcc)
     gene            complement(270..575)
                     /gene="trxA"
                     /locus_tag="PLH17_03450"
     CDS             complement(270..575)
                     /gene="trxA"
                     /locus_tag="PLH17_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOR20858.1"
                     /translation="MLKHIENQQQFDEVLKGKKVLVDFYADWCGPCKMLGPVLEEVSK
                     ELGDAVTIAKVNVDQQEAIAGQYSIFSIPTMILFENGQIARKQVGFVPKAALIRFIG"
     gene            complement(603..1502)
                     /locus_tag="PLH17_03455"
     CDS             complement(603..1502)
                     /locus_tag="PLH17_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOR20859.1"
                     /translation="MDSKLEKAQKLSSFWIKIIAFATMALDHIGVFMWQHVTSQSDAL
                     YIVGFIFRCIGRLSFPLFILLLVEGLIHSKSVGRYLLRLGLLLSLVLAVQIVLYYFID
                     PDVGVNPFIDLVISGTFIYLITKKDWKKYLALLPLAFVILSTTVDILERSSLNLVIFW
                     FPAYLRMGYSLFGFLMSLAFYYAYDLGKKVMFSANSKDEYVAETKELLKVPQYRSLVN
                     IIMAIALFFLNVLIWFLTYLSPSLDLYYADIQTWSLIAGLIIILYNGKRGYDKKWFRY
                     ASYAFFPAHIALIALIFALIFGI"
     gene            complement(1511..2368)
                     /locus_tag="PLH17_03460"
     CDS             complement(1511..2368)
                     /locus_tag="PLH17_03460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR20860.1"
                     /translation="MFKNKKKPRNYRKCPICQHLHDGSLDECPRCGQQSDGPVLTTRF
                     PNVIRVHWIKQIFLFLIGWGGLNVVSLIVSAFFVLYANNYIFGAIQRETFLSSHHVGI
                     MLNLITYSTLFVGILGLLWKDLLTILKTFKRLRAIVVGLAYGGAVMASVIIYNLIVIL
                     AGVEMSDNQNETAIVTMMTSYPVMSFVAFVILGPLVEEMTYRLGLFSLLHRLNRPLAY
                     VVTMLIFGLIHVSFNAETIVNELINLPSYIIAGTILTIAYEKEGFAVSTYAHITNNLI
                     SFLLTAFQG"
     gene            2466..3701
                     /locus_tag="PLH17_03465"
     CDS             2466..3701
                     /locus_tag="PLH17_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20861.1"
                     /translation="MDATTIELVVNIIKWGSLGLLILLVVILFFAGLIGWKRGIFNAG
                     FRLLFIGTLIIIALTTVRPMVDFIGGRDMGPLLRLFGMDGVTITMGSGSTTIQVASIF
                     GTLSDLLIALGEANGITVSAEQVEPVIHGLVYLILSTFVVIMDGILISSLGNLGATIL
                     WHALFKHLISKRIRKAVRVKGVAALMGATQTLVVGAMLIFPFSALINNVTAAFKNEDN
                     GITIDNEMINTVTTIVDAYEDSVLANVLFNWTDNSSGQSWDVMLMDSLTSADVNGLKV
                     SFIDEIYNIVGIGKTLISAGILSEDGMASIGQTLLANDEMVVSLLSSIGNSGLVLMIL
                     PVAVELALNLDVVKQYVGDGLLDVSDVDWKQEINNIGAMYTAVQKTGLFDDLFDEEGN
                     PVLDAEALKKSLSSTLIRG"
     gene            3698..8737
                     /locus_tag="PLH17_03470"
     CDS             3698..8737
                     /locus_tag="PLH17_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20862.1"
                     /translation="MMNIFKTIDDSKLLSRAIPAVAYTMLKDNPDMEAFSVFLPTEWD
                     DFAAIRWGSELSIVYDALFRLNALDETLLDALMSAGGGEGDGEPEGGEPEPAVQLALQ
                     NAKKGAPYMSAQDINNLLADEEEPEEEGGIPLEPILRNAGAIKEILIGRTDANGNPLG
                     VDENGVTRVFDEDGNRLYNRHYALFDSQLLQYSLSGAITYLVDSLFGTIDLGGADIDG
                     LIADLNTGVKLLNYKKEYGAVLDLMVALGENDATRALVLDFENMPGIVFNDDGGLESI
                     DPALISGLKIALPKIDNSRFLSLVFPSLLESTFGGDDMAGMFSAIGIDPAKLNWDTPR
                     LGREFALLIDAFDSVQELMDIMEPYKNESGDIPAENFGPLMFELSENDEPLAKMLDTL
                     YTSNIINEKYQENIYNTFGVLLHAKGEMVYGTNSNFYAMLDFVFDSLLQGRGFSNKKD
                     IYNVAKWTNSRTISGDFRRDRFGDAIYDGETGFMTEFISSIGRTGLLDIIDLEAGESI
                     DYGELGAAVGEVFDAVERSAVFSATFGDVLDMFVLDALSDDPGDVSFNNVSDWSLEGD
                     AFERLCLAIDEFGDIDFADIDFIGSDPDNVVELLSALADSQMFDGPDGYLFGDFFYDQ
                     LMTSLGGNAQYFYDPNATTTNEVKEDFDSLETRPEWQDEIVLFGAALEALQNANVDQY
                     GDPIDTGDPVDYLGLLTTGKVTADNVHGILTALNNTTTMRMIIYNGYNQIATAFASDE
                     IDLSTMNNIALVDMSKAQRQDEIDVTVKMYRVIENMGTQDPGTGEYSYNINLATTTDE
                     QVDDLRDALSSLVASVVFNSLDGDSLPADETVFKQFTRFFMRGPVIEEAIFRPDNPKD
                     IDGVSNSYYTDGASKTTYLLDTNFPTPSVDAGMELVFDAVKTEAAQKSYVQEMVDLVD
                     VMIHLDDGLGVGDPSPYSPDGLSITFDALTATNFEAVLNALNDSELLYDCVPNMISRI
                     VNDEAAFAIDDIELGAANPFFHYNGVNFTNRFPSGEISELSDLFILIQDFSNLVATGD
                     IDLGLIAGELTLFKDTLVALQETETFYRAGSYLPGEITVFEQIMRKIYTDTYLAELSY
                     NRVVDFDSSITSISEGIVKKITDSIVDFPNFANPLHGDSLHAGNWVTEIDAIGDLIAA
                     FDGLGMESGDFSGLSIDSLTPAQLETILQSINALDNVKDAIPFMIKDALYNENINFNR
                     FSTYNKDTYDLNAALYRLDGTYSNINLAESDTSIAGSQIDFLAGGSLYNTDPINNICS
                     LQIETTAAIDSFKVFYGNAVNPMTNYYILDAAESNVYTISLSCIAYQYFRIEAVTNFT
                     MDDLSVIQAIETGNYMQNQVSYRDTNIPILIDLMEEFYIDSSSTYYDFSQPEGVVGFV
                     NDGNSTGPILAFIVDSDLYQDPVTPSYKGEAVFLYNIMSFEMTQNIGGSPVTLDSELA
                     HNVRGASKDDKLAKIDDILNEDDGGRFDATKEGDAIDYGLFAMSFLEGAINIDTSKTA
                     FVSAQDYYLYNIARFDNGSEIVNYLDLAVGSSSRGAVDNNLLDDYGHALLISEIVAGQ
                     LDGILQNKYDYLDANAIVYTPVDLYDSYYDLLNATERVGLTGAVNALYYSLNSLIVDN
                     TEMTEAEITTAFENMFDSEISKIFYLADIYPCLSGNIFAGGRGLAVNPNPTTLADWQS
                     IAAALIADRGY"
     gene            8740..9348
                     /locus_tag="PLH17_03475"
     CDS             8740..9348
                     /locus_tag="PLH17_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOR20863.1"
                     /translation="MFAILNSSTSTLIIDKSRFIAVTFHVRNGEEVKRCLSAIKKEYP
                     KARHYCYAYIIGAEEKGFDDGEPARTAGRPLLELLKRGQFDETLIVVIRYFGGTLLGA
                     SRLLRAYVAVANEALSGAEKHEIVHLFSYALELAYSDYEYLLNEAKKRSYILENSVFS
                     DTIMIKLLAREPADDFLQQTLHGRGKITPLEREKRYLKETST"
     assembly_gap    9494..9593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9594..10451
                     /locus_tag="PLH17_03480"
     CDS             <9594..10451
                     /locus_tag="PLH17_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOR20864.1"
                     /translation="LEAELKIKDAFTTRDLRDELKEKYGELIIEDLHPRLTSLRMIKS
                     PAEVALIRDAIKTTDLGIKNVLLNLYPGQYEYNVRNTFEYAVRDDNNAEIAFDTIVAG
                     GQNAVILHYPNPKERLNNGDLVLLDLGARRHYYNADISRTFPINGKFLPKQKALYEIV
                     LGCNKAVINFIRPGLTLNELQKFATDYLAAECVLKGFIKNKEEITAVYYHGVSHHLGL
                     DVHDVADRDRPLEPGMVITVEPGLYFKQLKIGIRIEDDVLITETGCENLSKDILKDVS
                     DIEKIMSTK"
     gene            complement(10522..>12242)
                     /locus_tag="PLH17_03485"
     CDS             complement(10522..>12242)
                     /locus_tag="PLH17_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HOR20865.1"
                     /translation="NENLKTFTAKTNILGTDYYLTVPTSNAWTMTADSSTLLLETTYG
                     SGDASAVANSTTTDRHIRQNPTYGLRSYAGDTGAIAYFYKVQEIGKSLTSINVSGTPT
                     KTSYYAGESFDSAGITVTATYSDTSTLDVTNSCTFSPNPLTAGTTSVTVSYAEASVTK
                     TALINGITVQENTLEHISIKSPATNTVFPLGSTFSSSGLIITATYASGLSNDLTTGFE
                     VSVVDTNVLGQQTVDIAYGGQTTSYNVTITNQGANVTSGSGVFATDLFISEYIEGAVG
                     NNKAIEIYNGTGAAVSLANYTAALYPNGAANPTSTLVLSTFSATLGHGQVLTIVNSGA
                     TAAFKVGNYIESTITYFNGNDAVALLKNDTVIDLIGVIGADPGTAWTDGGSKSTANQT
                     LVRNSSIASPNATFTWSEWNSYPADTSTYVGSHTYEGGQASGDVTSEEQAIAWAQYFL
                     SSTDRICVADGSTNADDLQSGWSLLASEYGYMVDDAKDAFVDNGSSNATIASAKARYI
                     YIVEKYDFDNYVTDGSGFKMIPSNVVSYFGANDKLILIISMLLIVLGTTACAFIALKR
                     KQSDVI"
BASE COUNT         3233 a         2779 c         2909 g         3221 t
ORIGIN      
        1 aaagagacga tgtaatgacg cgttaatcaa acccatcaac aagccgctta agtgcggctt
       61 tttaatatac gaaaaagcac tcgactgagt gcttactttg cgactggagc aggcgacggg
      121 aatcgaaccc gcgtaattag cttggaaggc taatgttcta ccattgaact acgcctgcaa
      181 aaaaagaaaa tggctaacta gcaatggcgc tttcacgcga atgatatatt agcacattta
      241 ctttcttgat taaagagaga attgtctcgt tagccgatga agcggattaa ggccgctttc
      301 ggaacgaaac cgacctgttt gcgggcgatt tgaccgtttt caaaaaggat catcgtcgga
      361 atgctgaaga tgctgtattg accggcgata gcttcttgct gatcgacgtt gactttggcg
      421 atcgtaacgg catcgcctag ttccttgcta acttcttcaa ggacgggtcc gagcatcttg
      481 cacggcccgc accagtcggc gtagaaatcg acgaggactt tcttcccttt taagacttca
      541 tcaaactgct gttggttttc aatgtgtttt aacatattta tcaattcctt tcgtgaattt
      601 gtttatatac caaatattaa ggcaaaaatg agggcaatta aagcaatatg cgccggaaag
      661 aaggcatagc ttgcatagcg aaaccatttt ttgtcgtatc cgcgcttgcc gttataaaga
      721 atgatgatta acccggctat aagcgaccat gtctggatat cggcatagta taaatctaaa
      781 gaaggtgata aatatgtcaa aaaccagatt aagacattga ggaagaagag ggcgatggcc
      841 atgatgatgt tgacgagact tcggtattgg ggaaccttga gcagttcttt ggtctcggcg
      901 acatattcat ctttgctgtt cgcgctgaac atcacctttt tgccgagatc ataggcatag
      961 taaaacgcta ggctcatcag gaagccaaag agcgaatagc ccatccttag gtaagccggg
     1021 aaccagaaga tcaccagatt tagcgaactc ctctccaata tgtcgacggt cgtcgaaagg
     1081 atgacgaatg caagcggcag aagagcgagg tattttttcc aatctttttt ggttattaaa
     1141 taaataaatg ttccgcttat gactaagtcg atgaaaggat tgacgccgac atcgggatcg
     1201 ataaagtagt agaggacaat ctgaacagct aaaactaagc tgagcaagag gccaagcctc
     1261 aacaagtaac ggccaacgct cttggaatgg attaagcctt cgacgagcag gaggataaat
     1321 aaaggaaaag agaggcggcc gatacaacgg aagataaagc cgacgatata caaggcatcg
     1381 ctttgcgatg tgacatgttg ccacatgaag acgccgatat gatcgagagc catcgtcgca
     1441 aaggcgatga tctttatcca gaagctcgat aacttctggg ctttttctaa tttggaatcc
     1501 atgggttctc ctagccttga aaggcggtca gcaagaagct gatcaggttg ttcgtgatat
     1561 gggcataggt cgagacggcg aatccttctt tttcatatgc gattgtaagg attgtgccgg
     1621 cgataatgta ggacggaaga ttgattaact cgttaacgat cgtctcagcg ttaaatgaca
     1681 cgtggataag accgaagata agcatcgtca cgacataggc tagggggcgg ttcaagcggt
     1741 ggagcaaaga gaataaaccc aggcggtaag tcatttcctc gacgagcgga ccgagaatga
     1801 caaaagcgac aaacgacatg accggataag aagtcatcat cgtgacgatg gccgtctcgt
     1861 tttgattgtc gctcatctcg acgccggcca ggatgacaat taaattataa ataatgacgg
     1921 aggccatgac ggcgccgcca taggcaagtc cgacgacgat tgctctgagg cgcttgaaag
     1981 tttttaaaat agtcaataaa tctttccaaa gcaaacccaa aataccgacg aaaagcgtcg
     2041 aataagtaat taaattaagc atgattccga cgtgatgaga ggaaagaaaa gtttcgcgct
     2101 gaatagcgcc aaaaatataa ttattggcat acagcacaaa aaatgccgaa acgatgaggc
     2161 tgacgacatt gagaccgccc cagccgatca gaaaaaggaa aatttgtttg atccagtgaa
     2221 cacggatgac atttgggaaa cgcgtggtca agacaggacc atcgctttgc tggccgcagc
     2281 gcgggcattc gtccaaagac ccgtcgtgca gatgctgaca gatgggacat tttcgataat
     2341 tgcggggctt tttcttgttt ttgaacatta tgaggagttt atggtgttta agcaaaatat
     2401 gtaaataact ttaaggaagt tatgcaataa taatataata aaagcaaggt gggtgcacaa
     2461 acaatatgga tgcaactaca attgaactcg tcgttaatat aattaaatgg ggcagtctcg
     2521 gactcttgat cctgctcgtc gtcattttgt tttttgccgg attaatcggc tggaaacgcg
     2581 gcatctttaa tgctggcttt cgcctccttt ttatcggcac gcttatcatc atcgctctga
     2641 cgacagttcg cccgatggtc gattttatcg gcgggcgcga tatgggtccg cttctcaggc
     2701 tgtttggcat ggacggcgtg acgattacga tggggtcggg ttcgaccacc attcaagtcg
     2761 cgagcatctt cggcacccta agcgaccttt taattgcgct tggcgaagcc aacggaatca
     2821 ctgtttctgc tgagcaggtc gaacccgtca ttcacgggct cgtctatttg attctttcga
     2881 cgtttgtcgt cattatggac ggcattctca tctcgtcttt gggcaatctc ggcgcgacca
     2941 tcctgtggca cgctttattt aaacatctta ttagcaagcg cattcgcaag gccgtgcgcg
     3001 tcaaaggcgt ggccgccctg atgggtgcga cgcagacttt ggtcgtcggc gcgatgctca
     3061 tctttccttt ctcggccctg attaacaatg tcacggcggc ctttaaaaac gaagataacg
     3121 gcatcactat cgataacgag atgatcaata ccgtgacgac gattgtcgat gcctatgaag
     3181 atagtgtttt agcgaacgtt ttatttaact ggaccgataa ttccagcgga caaagctggg
     3241 atgtcatgct catggattcg cttacttccg ccgatgtcaa cggcctcaaa gtcagtttta
     3301 tcgatgagat atataatatt gtcggaattg gcaaaacctt gatttccgcc ggcattttaa
     3361 gcgaagatgg catggcatca atcggccaga ctctattggc aaatgatgag atggtcgtct
     3421 ctcttctatc ctccatcggc aattccggac tagtgttgat gattcttccg gtcgcggttg
     3481 agctggcgct taatctcgat gtcgtcaagc agtatgtcgg cgacggtctt ctcgatgtca
     3541 gcgatgtcga ttggaagcaa gagatcaaca atatcggcgc gatgtacacc gccgttcaga
     3601 aaaccggtct tttcgatgac ttatttgacg aagaaggcaa tccggtactc gatgccgagg
     3661 cccttaaaaa atctttgagc tcgacactta ttcggggatg atgaacatct tcaaaacaat
     3721 cgatgacagc aagctcctca gccgcgccat tccggctgtc gcttatacga tgctgaaaga
     3781 taatccggac atggaagcat tttcagtttt cttgccgacg gaatgggatg attttgccgc
     3841 cattcgctgg ggctcggagc tgagcattgt ctatgacgct ttatttcggc tcaatgctct
     3901 tgatgagacc ttgctcgacg ctttgatgag cgccggcggc ggagagggtg atggtgaacc
     3961 cgaaggcggt gaaccggagc cagctgttca gctcgccctt caaaacgcca aaaaaggcgc
     4021 cccctacatg tccgcgcagg atattaataa tctgttagct gacgaagaag aaccagaaga
     4081 agagggcggt ataccgctcg aaccgattct tcgcaatgcc ggcgccatta aagaaatatt
     4141 aatcggtcgg actgacgcta acggcaatcc cctcggcgtc gatgaaaacg gagtcacccg
     4201 cgtctttgac gaagacggta accgcctcta taaccgccac tatgctctct ttgattcgca
     4261 gctcttacaa tattcgcttt cgggggcaat tacatatctc gtcgattctc tgttcggaac
     4321 tattgattta ggcggtgccg acatcgatgg cttaatcgcc gatttaaata ccggcgtcaa
     4381 gctgctcaat tacaaaaaag aatatggcgc cgttctcgac cttatggtcg cattaggcga
     4441 aaatgatgcg acgagagccc tggtcctcga ttttgaaaat atgccgggca tcgtttttaa
     4501 cgatgacggc ggtctcgaaa gcatcgatcc cgctttgatt agcggtctta aaatagctct
     4561 tcccaaaatt gacaactcgc gctttttatc gcttgttttc ccgagccttt tggaatcgac
     4621 ttttggcggc gatgacatgg ccggtatgtt ctcggccatc ggcatcgacc ccgccaagct
     4681 caattgggat accccgcgcc tcggacgcga gtttgccttg ctgatcgatg cctttgatag
     4741 cgttcaggaa ttgatggaca tcatggagcc gtataaaaat gaatccggag atattccggc
     4801 ggagaatttc ggacccctga tgtttgagtt atccgaaaat gacgaaccgc tcgccaagat
     4861 gctcgacacc ctttatactt ccaatatcat caacgaaaaa taccaggaaa atatttataa
     4921 taccttcggc gttctcctcc acgccaaagg cgaaatggtg tacggcacga attcgaactt
     4981 ctatgcgatg ctcgacttcg tcttcgacag cctcttgcag ggcagaggtt tcagcaataa
     5041 aaaagatatt tataatgtcg cgaagtggac gaattcacgg acgataagcg gcgacttccg
     5101 ccgcgaccgc ttcggcgatg ccatctatga tggcgaaact ggttttatga ccgaatttat
     5161 ctcgtcaatc ggccgcaccg gcctcctcga tatcatcgat cttgaggctg gcgaatcaat
     5221 tgactatggc gagcttggcg cggctgtcgg cgaagtgttt gacgccgtcg aaagaagcgc
     5281 ggtcttctcc gcgacttttg gcgatgtcct cgatatgttt gtcctcgatg ccctgagtga
     5341 cgatcccggt gatgtttcgt ttaataatgt cagcgactgg tcgctggaag gcgatgcttt
     5401 tgaaaggctg tgcctggcca tcgatgaatt cggcgatatt gattttgctg acatcgattt
     5461 tatcggaagc gatcccgaca atgtcgtcga actcctcagc gccctggcgg attcgcagat
     5521 gtttgacgga cccgacggct acttgttcgg cgatttcttc tatgatcaac tgatgacttc
     5581 attaggaggc aatgctcaat acttctatga tcctaatgcg acgacgacga acgaagttaa
     5641 agaagatttt gattcccttg aaacgcggcc ggaatggcaa gatgaaattg tcttgttcgg
     5701 cgcggctctt gaggctttgc aaaacgcgaa tgtcgatcaa tatggtgatc cgatcgatac
     5761 gggtgacccc gtcgattatc tcggtttgtt aacgaccgga aaagtgaccg ccgacaatgt
     5821 tcacggcatt ctgacggcgc ttaacaacac gacgacaatg cgcatgatca tctataatgg
     5881 ctacaaccag atcgccacgg ctttcgcctc tgacgaaatt gatttatcga cgatgaataa
     5941 catcgctctt gtcgatatga gcaaagctca aaggcaagat gagatcgatg ttaccgtcaa
     6001 gatgtaccgc gtcatcgaaa acatgggcac gcaagatccg ggcaccggcg aatattcgta
     6061 taacattaat ttggcaacca cgaccgacga gcaggtcgac gacttgcgcg atgccctcag
     6121 cagcctcgtc gcttccgtcg tcttcaactc tctcgatggc gattcactgc ccgccgatga
     6181 gacggtgttt aaacaattca cgcgtttctt tatgcgcggt ccggtcattg aagaagcgat
     6241 tttccgtccc gataacccca aggatatcga tggcgtaagc aattcttact ataccgatgg
     6301 agccagtaag acgacttatt tattagatac taatttccca actccgagcg tcgatgccgg
     6361 catggaactc gtctttgatg cggtgaagac tgaagccgcc cagaagagtt atgttcagga
     6421 gatggtcgat ttagtcgatg tcatgattca tcttgacgat ggattgggcg tcggcgatcc
     6481 gagcccgtat tctccggatg gattaagcat tacttttgat gcgttgaccg cgactaactt
     6541 tgaggcggtt ttaaacgcct tgaatgactc ggaactcctt tatgactgcg tgccgaatat
     6601 gatttcgcgc atcgttaatg atgaagcggc gtttgctatc gatgatatcg aactaggcgc
     6661 cgccaatccg ttcttccatt ataacggcgt caacttcacg aatcgctttc cgagcgggga
     6721 aattagcgaa ctgagcgatc tctttatatt aattcaagac ttcagcaatt tagtcgccac
     6781 cggtgatatc gatctgggtt taattgctgg cgaactgacg ctttttaaag acacattagt
     6841 cgcccttcag gagacggaga cattctatcg ggccggaagc tatttgcccg gagaaattac
     6901 cgtcttcgag cagattatgc gcaagattta taccgacacc tatttagcgg aattatccta
     6961 caaccgcgtc gttgattttg attcatcaat cacttcgata tcagaaggaa tcgtcaaaaa
     7021 gattaccgac agcatcgttg atttcccgaa tttcgctaat ccgttgcacg gcgattcgct
     7081 gcacgccggc aattgggtta cggagattga tgccatcggc gatttgatcg cggcttttga
     7141 tggtctggga atggagtcgg gtgacttctc gggtctctcg atcgattcct tgacacccgc
     7201 ccaactagag acgattcttc agagcattaa cgctcttgac aatgtcaaag acgccattcc
     7261 gtttatgatt aaagatgctc tctataatga aaacatcaac tttaatcgtt tctcaacgta
     7321 taataaggac acctatgatt taaatgccgc attatataga ctcgacggca catatagtaa
     7381 catcaactta gcagaaagcg ataccagcat tgctggttcg caaatagatt tcttagccgg
     7441 cggttcactt tataataccg atccgatcaa caatatttgt tcccttcaaa tcgagacgac
     7501 ggccgccatc gatagtttta aagtgttcta cggaaacgct gttaatccga tgacaaatta
     7561 ctatatcctc gatgctgccg aaagcaacgt ttatacaatc agcctcagct gcattgcata
     7621 tcaatatttc cgcattgagg cggtcaccaa ctttacgatg gatgacttaa gcgtcattca
     7681 ggcgatcgaa accggcaatt acatgcaaaa ccaagtcagc taccgcgata ccaacattcc
     7741 gattctgatt gatctaatgg aagaattcta tattgattca tcgagcacct attatgattt
     7801 cagccagccg gaaggcgtcg tcggctttgt caatgatggc aactccaccg gtccgattct
     7861 ggctttcatc gtcgatagtg atttatatca agatccggtc acgccaagct acaaaggcga
     7921 agccgtcttc ctctacaata tcatgtcatt tgagatgacg cagaatatcg gcggaagccc
     7981 ggtcactttg gatagtgagt tagcgcataa tgtccgcggt gcatcgaaag acgataaact
     8041 tgcgaaaatc gatgacattt tgaacgaaga tgatggtggt cgctttgatg ccacaaaaga
     8101 aggcgatgcg attgactatg gcttatttgc catgtccttc cttgaaggcg ccatcaatat
     8161 cgatacgagc aaaaccgctt ttgtttcagc ccaggattat tatctttata atatcgcccg
     8221 attcgacaat ggttcagaaa tcgtcaacta tctcgatttg gcagtcggtt cgagctcgcg
     8281 aggcgcagtt gataataatc tcctcgacga ttacggacac gccttgctga tcagcgaaat
     8341 cgtcgccggg cagctcgacg gcatactgca gaataaatat gattatctgg atgctaatgc
     8401 gattgtctat acccccgttg atttatatga tagctattat gatttgctga atgccacgga
     8461 acgcgtcggt ttgacgggcg ccgtcaatgc tctttattac tcgcttaatt cgctgattgt
     8521 cgataatacg gagatgaccg aagccgagat tacgaccgct ttcgaaaaca tgtttgattc
     8581 ggaaatttcc aaaatctttt atctggccga catttatccg tgtttaagcg gcaatatttt
     8641 cgccggcgga cgcggtttag ccgtcaatcc caatccgacg acgctggctg actggcagag
     8701 catcgccgcc gcgttaattg ccgatcgcgg ttattagtga tgtttgcaat tcttaatagt
     8761 tcaacctcga ctcttatcat agataagtca cgctttattg ccgtgacttt tcatgttcgt
     8821 aacggcgagg aagttaaacg atgcttaagc gctataaaaa aggaatatcc gaaggccaga
     8881 cattactgct atgcctatat catcggtgcg gaagagaagg gctttgatga tggcgagccc
     8941 gcccggacgg ctgggcgccc tttgctcgaa cttctgaagc gtggccagtt tgatgaaaca
     9001 ctgattgtgg tcatccgcta tttcggcggg acgctcctcg gggcatcgcg gttgctaaga
     9061 gcgtatgtcg ctgtcgctaa cgaagcgctt agcggtgccg aaaaacatga gattgttcac
     9121 ctcttttctt acgcgctgga actcgcctac agcgattatg aatatttgct taacgaagca
     9181 aaaaagcgct catacatctt ggaaaacagc gtctttagcg ataccataat gattaagctt
     9241 ctcgctcggg agcccgctga tgatttttta cagcaaacgc tacacggaag gggtaaaatc
     9301 accccgctcg agagagaaaa acgttattta aaggagacct caacatgata tctgccagcg
     9361 aatacagcca aaggcgcgac cgcctcttcg agatgatgga cgacgattct tttctcgtca
     9421 tctatgcagg cgtgggacgc aagatgagcg gcgatcattt cgcctatacc gtcaaccgca
     9481 atttttacta tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcgaag
     9601 ccgagctcaa aattaaagat gcctttacga cccgcgatct tcgcgacgag ttaaaagaga
     9661 aatacggcga actgataatc gaagatcttc atccgcggct cacatcgctg cgaatgatta
     9721 aatcacccgc ggaagtcgct ctcatccgcg acgcgataaa aacgacggat ttaggcatta
     9781 agaacgtcct tttaaactta tatcccgggc aatatgaata caatgtgcga aatacttttg
     9841 aatacgcagt tcgcgatgat aataatgccg agattgcctt tgacaccatc gtcgccggcg
     9901 ggcaaaacgc cgtcatcctc cactatccga atccgaagga aaggcttaat aacggcgacc
     9961 tcgtcttgct cgatctcggg gcgagaaggc attactataa cgccgatatc tcgcgtactt
    10021 ttccgattaa cggcaaattt ttgccaaagc agaaagctct ctatgaaatt gtcttaggct
    10081 gcaataaggc cgttatcaac ttcattcgtc cgggtttgac tttaaacgaa ctccagaagt
    10141 ttgcgaccga ctatctggcc gcggaatgcg tcttaaaagg tttcattaag aacaaggaag
    10201 aaattacggc cgtatactat catggtgtct cacatcatct cggcctcgat gttcacgatg
    10261 tcgccgaccg cgaccgcccc cttgagccag gaatggttat tacggtggaa ccaggacttt
    10321 atttcaaaca gctgaaaata ggcattcgca tcgaagatga tgttttgatt accgagacgg
    10381 gctgcgagaa cctatcaaaa gatattctca aggatgttag tgacatcgag aagatcatgt
    10441 caaccaaata aaagagaaca aaagagagcg accgatgaag cgctctcttt ttttgtttat
    10501 tagcaaactg acaacaaatg actaaatgac atcggattgt ttccgcttta aggcgataaa
    10561 cgcacacgca gttgttccaa gaacaattaa aagcatcgaa ataatgagga tcaatttatc
    10621 atttgcacca aaataactta cgacattaga aggaatcatt ttgaatccgc ttccgtcggt
    10681 aacgtaattg tcaaaatcgt atttttcgac aatatagata tatcttgctt tggcacttgc
    10741 tattgtcgca ttgcttgaac cgttatcgac aaaggcgtct ttggcgtcat cgaccatgta
    10801 tccgtactcg ctagcgagta gcgaccagcc actttgcaaa tcatcagcat tcgttgaacc
    10861 atcagcgaca caaatccgat cggttgatga taaaaaatat tgagcccagg caatcgcctg
    10921 ctcttcgctt gtgacatcgc cagaagcttg cccgccttca taagtgtgag aacccacata
    10981 agttgatgta tcggctggat atgaattcca ttcagaccaa gtaaacgtcg catttggtga
    11041 tgcgatactg ctatttctga ccaatgtctg attggcggtg ctttttgagc ctccgtctgt
    11101 ccaggcggtt ccggggtcgg cgccgattac tccgataagg tcaataaccg tatcgttttt
    11161 aagcaaagcg acggcgtcgt ttccgttaaa ataagtgatg gttgattcta tatagttacc
    11221 aactttgaac gcggcagtgg cgcccgaatt gacaatagtt aggacttgtc cgtgtccgag
    11281 tgtcgccgaa aaagttgata aaaccaaggt cgaagtagga ttagcggctc cgttaggata
    11341 aagtgccgcc gtataattag caagcgagac ggccgctccg gttccattgt atatttcgat
    11401 tgctttgtta ttgccgacag cgccttcgat gtattcggaa ataaataagt cggtcgcaaa
    11461 gacgccagaa ccgcttgtta catttgcacc ctgattcgta attgtcacat tataagatgt
    11521 tgtttgccct ccgtaggcga tatcaactgt ttgctgaccg agaacattcg tgtcaactac
    11581 agaaacttcg aagccggtcg ttaaatcatt tgacaatccg cttgcgtatg tcgcagtaat
    11641 gattaaaccg ctggagctga aagtgctgcc aagagggaaa acagtgtttg ttgcgggact
    11701 ttttatcgat atatgttcaa gggtattttc ctgaaccgta attccgttta ttaatgcagt
    11761 ttttgtcact gatgcctcgg cgtatgaaac ggtgacactc gtggtgccgg ccgttaatgg
    11821 attgggtgaa aaagtgcatg agttcgtgac atctaatgtt gatgtgtcgc tataggtggc
    11881 tgtgacagtt attccagcgg aatcaaatga ttctccggcg tagtatgatg tttttgttgg
    11941 tgtaccagaa acattgatgc ttgtcaggga tttgccgatc tcctggactt tataaaaata
    12001 tgcgatagcc ccagtgtcgc cagcatacga ccgtaatcca tatgttgggt tttgacgaat
    12061 gtgtcgatct gtagtagttg aattagcaac tgcactagca tctcctgaac catatgttgt
    12121 ttctaacaac agcgttgacg aatcagcggt catcgtccag gcattgcttg tgggcacagt
    12181 taagtaataa tcggtaccta aaatatttgt tttggcggta aatgttttta agttctcgtt
    12241 aa
//
LOCUS       DAOSSL010000027        11555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5536_length_11555_cov_18.070348, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000027 DAOSSL010000000
VERSION     DAOSSL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5536_length_11555_cov_18.070348"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLH17_03490"
     CDS             complement(<1..143)
                     /locus_tag="PLH17_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOR20866.1"
                     /translation="MNSNIVNEVTASQLKSDLIDFSSGDTVKVFVRIIENKKERLQAY
                     EGVV"
     gene            358..642
                     /gene="rpsP"
                     /locus_tag="PLH17_03495"
     CDS             358..642
                     /gene="rpsP"
                     /locus_tag="PLH17_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOR20867.1"
                     /translation="MSVKIRLTRVGRHKDPFFRIVVADSRYSRDGRYIEQLGFFDPAK
                     GIENAVIDEEVTLKWLRQGATPSDTIRTLFTKKGLMAKLAESKKSVKGDK"
     gene            642..905
                     /locus_tag="PLH17_03500"
     CDS             642..905
                     /locus_tag="PLH17_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOR20868.1"
                     /translation="MDYEKIIHALIDPIVENKDIIMIRELPSDSSREVVLLIVAESDD
                     TARLIGRKGAIANALRELISVAGKSENKRIHLKFESFEQKDED"
     gene            926..1435
                     /gene="rimM"
                     /locus_tag="PLH17_03505"
     CDS             926..1435
                     /gene="rimM"
                     /locus_tag="PLH17_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOR20869.1"
                     /translation="MEQVSIGKITNTVGLRGEVKVFVTTDFPSKRFKKGQLVTLLNEE
                     KDERVEAVIASARQSKDRYVVSFKNITSIEEAEKLIGLLVLIEKKVENLPEGFYYHAD
                     LIGCGVYDDGNNLIGTVAKVEDYPAHRTLRITRNDGPDVLIPFIAAFIKSVNIEKKEI
                     VAHIIEGML"
     gene            1432..2205
                     /gene="trmD"
                     /locus_tag="PLH17_03510"
     CDS             1432..2205
                     /gene="trmD"
                     /locus_tag="PLH17_03510"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOR20870.1"
                     /translation="MKITILTLFPQMFDGFLTNSIIKRAISKKAAEIVLLNIRDYTKD
                     RYRRVDTPPVGGGAGLIMKVQPLVDAIKDAKSANSHVILLSPRGKTYTQNTAKRLANC
                     DEIVLICGHYEGVDERVSAYVDEEISIGDFVLTGGEIAAMAIADSLIRLQKGAITSSS
                     LDEESFESGMLEYPQYTEPYDYEGRRVPDILYSGNHAAISKWRKKQALYITRLYRPDL
                     FAQLKLGRSDIKLLHEIDKNTEPEWEKKALEKGAKFIKK"
     gene            complement(2209..3564)
                     /gene="ffh"
                     /locus_tag="PLH17_03515"
     CDS             complement(2209..3564)
                     /gene="ffh"
                     /locus_tag="PLH17_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOR20871.1"
                     /translation="MAFESLSERFARIFKKLRGQSRLTESNMDDMLKEIRVALLEADV
                     NFKVVKDFLAEVKKRAIGEEVLTKLNPSQTIVKIVHDEIEKLLGSSDDQLTFNRNKPT
                     LIMLVGLQGSGKTTTAGKLALLMKGKMNKKVLLAACDTYRPAAIDQLEQIAGDIAVDI
                     IAERNKLSPVEIAKKARLKAFNDHYDVLIIDTAGRLHLDEPLMKELNDIIGEVEPDEI
                     LLAFDAMSGQDVANVASSFNARLKLTGAVMTKLDGDARGGAALSIRHLTGVPIKFSGT
                     GEKLIDIDVFHADRMADRILGMGDVVSLVEKVQEELDEREMKKSVNRMMSGKFDLEDM
                     LSQMKQIQKLGSLGGLMSMIPGMPKLSDEQKAQAEREIANFEAIYNSMTAEERRHPEI
                     LRNSRKVRIARGSGKTNADINRIINKYEKSKEMMRKMGQMQKGNKMPPGGLGGFGGLG
                     F"
     gene            complement(3554..3868)
                     /locus_tag="PLH17_03520"
     CDS             complement(3554..3868)
                     /locus_tag="PLH17_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20872.1"
                     /translation="MKDNLERTKKYSELMEFYSRLLTTSQQQIMSDYYLFDLSLGEIS
                     ENRKISRSAVLDTIAKTAKKLEKFEGKLGLVKLFKESKEKSRGQVLAALEELEGKIKH
                     GI"
     gene            complement(3865..4842)
                     /gene="ftsY"
                     /locus_tag="PLH17_03525"
     CDS             complement(3865..4842)
                     /gene="ftsY"
                     /locus_tag="PLH17_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOR20873.1"
                     /translation="MGLFTYLKDKFSRKSEKTTKYVSGLEKSRHHFADRLKVLTKRYA
                     AVNEAYFEELETILIEADVGIALTIRTIENLLKEAREKKISDPSVINELLVDALFVSY
                     ATKGGDIENEIQFQKNVPTVMLMVGVNGVGKTTTIAKIAHRYLSQGKKVMLAAGDTFR
                     AGATEQLEVWAKRLNCPIVIGKPAQDPASVAYEASQRAIKDKIDLLIVDTAGRLQTKL
                     NLMAELGKISRVLAKEIAGAPHETFLVIDATTGQNGVLQAKAFKEATPVSGIVITKMD
                     GTSKGGIILAIRDELALPVRFIGLGEKMDDLQEFDLDEYLYGLCLGDER"
     gene            complement(4842..5531)
                     /gene="rnc"
                     /locus_tag="PLH17_03530"
     CDS             complement(4842..5531)
                     /gene="rnc"
                     /locus_tag="PLH17_03530"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOR20874.1"
                     /translation="MHRLAKLFKYFHLKPKELRTYELAFTHPSYNLDAKSCHEDYERL
                     EFLGDSILGASVADLAFQAHPDKDQGFLTKLRSSLVNTKALAKYARYHHLAEFIVVGN
                     SFSGEVAKSDKMLENVFESFIGAVYLDLGFKVAYGIVKRVFEHDILNYTPEKTIDYKS
                     RLQEETQLEHRHSVTFRQVSVDGPAHNRVFEMEVVLDGGVVLGRGTGSSKKAAEQEAA
                     KNALEKKAGSK"
     gene            complement(5515..6504)
                     /gene="plsX"
                     /locus_tag="PLH17_03535"
     CDS             complement(5515..6504)
                     /gene="plsX"
                     /locus_tag="PLH17_03535"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOR20875.1"
                     /translation="MIKIVIDMMGGDLGSAAAVEGVLAFLKDHRDVEVTAVGKIEELT
                     LLQGKATLVDAREVLPMEAGVMDAIRAKGASMAIAIDQVLAQNADALVSSGSTGAYLG
                     STSIRLKKLPGVLRPALISPFPTKNLGQQVVILDIGASNENSASELVQFALMGKLYAQ
                     AVLEVKEPRVYLLSNGSEPEKGSPESKDAYQLLAGSFPGFMGNIEARDALNGTADVVV
                     TDGFTGNVFLKSCEGVAKMMSGFIKGAFMRNIFSKIGYLLSKKGFERMSEAMNYKNYG
                     GAMFLGLSKVAVKAHGSSDGLAFMHALRVAYRLAQADVPALIEKGIKENAPLS"
     gene            complement(6573..8570)
                     /locus_tag="PLH17_03540"
     CDS             complement(6573..8570)
                     /locus_tag="PLH17_03540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOR20876.1"
                     /translation="MKFSKSPRINKVLYDMGLYSFEDIIFHLPRKYDNLEPTREFGLR
                     DKERVVFFGRVTRPPVNSRFSRAQVTRFSFTTKAGNTFAVEAWNRPYLTKMLDSESVY
                     TLVANYDYYKNKLNMINLTKGEPPAGDYLRPVYTLPRAITNYQFSHLVEKAFDGKEEA
                     LESDIPAPLQEKYRLLNKEKALHLVHRPFSAKDIYEGLRVLKYEECLLFSLKTQIIRS
                     QNKELIKNEKQFIDRTKLKTFIRSLPYKLTEDQISSVAEIVKDMDQRSLMYRLLQGDV
                     GTGKTLVAALAFYGNFLRNDQGALMAPTDALARQHFRTLRELFKDTPVRLALLLGATP
                     KKERFQINMGLINHKIDVVVGTHALFSEDVTFSSLGLAVIDEQHRFGVNQRLLLASKG
                     EHADLLLMSATPIPRTLALTLYGDLDVSTLTQFPFSQRKIMTKVVSNSDSLIHKTIKQ
                     AINKQQRVYVVAPVIDISSTGLVSVEELFKYYEKLYPGQVSLLHGQMSIDDKNYALGE
                     FISGKTPIIVSTSVIEVGIDVKEASAMVIYAPTSFGLASLHQLRGRIGRDGKEAICLL
                     VYDGEDEDEINKLNVLVESNDGFFIAEEDLRRRGPGELSGLRQSGITNFEFVNLIDDF
                     KMFEYARNDAKTILERPFETSYRRIIARAKREIAQAKFTNV"
     gene            complement(8636..10288)
                     /locus_tag="PLH17_03545"
     CDS             complement(8636..10288)
                     /locus_tag="PLH17_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOR20877.1"
                     /translation="MKNIDGQLLKQMIISGTNNLYNHYPEVDSLNVFPVPDGDTGMNM
                     NLTMSSGMKEIQNRNDSEVYPIAVAFSKGLLMGARGNSGVITSQIFRGFAQSLEGKKV
                     MNAVDLAEAFVNGSEVAYKAVIRPVEGTILTVIRESSKAMLAHVNSDFAIEKAFAFLL
                     NEAKASLQRTPELLPILKEVGVVDSGGFGLVTIFEGMERALLGDVVEKSQATVFEERM
                     PQPEFKGERSEDEFGYCTEFILRLGPDAEKKPFVEKRFTGVLNSHGNSLVVVKDDDIV
                     KVHVHTLEPGSILNYAQNFGEFVTLKIENMTEQHSHLDIVPGEEAKPAVEEKPLKDYA
                     LIAVSAGDGLNQIFSDIGVDYIVSGGQTMNPATEDFLTAIEKVHARNVYILPNNSNIV
                     MTASQACDVIEGETIARVVPSKTIPQGIVAAMMFNPDLDPEENFKEMKAALKNVVSGS
                     VTYAIKDTDIEGIHVTKGYYMGFRNKSIIVCVKDKIEALHELLKAMIDENAAILTVIL
                     GEDVTESEESKLSESLFEAYPELEIDIKRGQQPVYSFLIGVE"
     gene            complement(10301..10660)
                     /locus_tag="PLH17_03550"
     CDS             complement(10301..10660)
                     /locus_tag="PLH17_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOR20878.1"
                     /translation="MTIDKSTPYGDINISTEAIATIAGNAAIECYGVVGLASKSSIRE
                     NIAELLKSDSYAKGVQVIKSKDAYEVDVYIVVAYGVKITEIVSQVQKRIKYVLEKTFD
                     LNFKAINVYVQSLKTIN"
     gene            complement(10776..>11555)
                     /locus_tag="PLH17_03555"
     CDS             complement(10776..>11555)
                     /locus_tag="PLH17_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20879.1"
                     /translation="IASDLNRDLAYKRYKVAYINIDYDEFVGEIDKKSYADDEKVRQI
                     RKKLANNVYLEGALHIYGAIKRLILKYDLHGFSIRCFDLLTIYRNTACLALAMLNNEG
                     ISAGCEGDLPLLLAMHLVKRLLSLDSFQANPSSIDEAKQTITFAHCTVPFSMCQSHVF
                     MTHFESGLGIGVRGELPLGPCTVFRLSGNLSKALIEEGEIVANLALENLCRTQIVVKM
                     NSGLEKILRHPLGNHHLIMFGHHKQRLGTALKALDPNLELI"
BASE COUNT         2870 a         2689 c         2820 g         3176 t
ORIGIN      
        1 acgacgcctt cgtaagcctg gagacgttct tttttgtttt cgatgatacg cacgaaaacc
       61 ttgacggtgt ctccggagct gaaatcaatg aggtcggatt tgagttgtga agcggtaact
      121 tcgttaacga tgttggaatt cattgtttat tccctccttt tcatgtggtt tcttaaggcg
      181 ttcatgtccc gatttttgac agcggaccgt ccgcagcgca ttttaacgcg cttagttata
      241 ttaccaaata agtatttggt tgacaagaaa catttattgc gtgtttgctg tcaagtattt
      301 ttgcttgaca ccaaatctta tttataataa actttcgatg gaaaaaggag aaagcaaatg
      361 tcagtaaaaa tcagattaac cagagtcggt cgccataaag atccgttctt ccgcatcgtc
      421 gtcgccgatt cgcgctactc acgcgatggc cgctacatcg aacaactcgg cttcttcgat
      481 cccgccaaag gtatcgagaa tgctgttatc gacgaagaag tgactcttaa atggctgcgt
      541 cagggcgcca ctccatccga cacgattcgt actctattta cgaaaaaggg tttgatggct
      601 aagctcgccg aaagcaagaa aagcgtcaaa ggagacaaat aatggattac gagaagatta
      661 ttcacgcctt gattgatccg atcgtcgaaa acaaagacat catcatgatt cgtgagcttc
      721 ccagtgactc ctcgcgcgaa gtcgtcttgc tcatcgtcgc cgagagtgat gacaccgctc
      781 gtctcatcgg ccgcaaggga gcgattgcca acgctcttcg cgaactgatt tcagtcgccg
      841 gcaaaagtga aaataaacgc attcatctca aatttgaatc gtttgaacag aaggacgagg
      901 attaatcctc gttctctttt gtcttatgga acaagtatca atcggcaaga ttacgaatac
      961 ggtcggactt cgcggtgaag tcaaggtttt cgttaccacc gatttcccga gcaaaagatt
     1021 taaaaagggg cagctcgtta cgctcttaaa tgaagaaaag gatgaacgtg ttgaagcggt
     1081 gattgcaagc gctcgtcaaa gcaaagatcg ctatgtcgtc tcgtttaaaa acatcaccag
     1141 catcgaggag gccgagaaat tgattggcct gcttgtgctg attgaaaaaa aggttgagaa
     1201 tcttcccgaa ggtttctatt atcacgccga tcttatcgga tgcggtgttt atgacgatgg
     1261 caacaatctc atcggcaccg tcgccaaagt tgaagattat cccgcccacc gcactttgcg
     1321 aatcacgcgg aatgatggcc ccgatgtgct cattccgttt atcgccgcct ttatcaaaag
     1381 tgtgaatatt gagaagaaag aaatagtcgc ccacatcatc gaaggtatgc tatgaagata
     1441 acgattctca ctctctttcc gcaaatgttt gacggctttt tgacaaactc gattattaag
     1501 agggccatca gcaaaaaagc cgccgaaatt gttctattaa acattcgcga ctacaccaaa
     1561 gaccgctatc gccgtgtcga caccccaccg gtcggcgggg gcgcgggctt aatcatgaaa
     1621 gtacagcctc tcgttgatgc cattaaagat gcaaaaagcg cgaacagcca cgttattctt
     1681 ttaagtcccc gcggcaaaac ttatacgcaa aatacggcga aaagactcgc aaattgcgat
     1741 gaaattgtac tgatttgtgg acattatgaa ggtgtggacg aacgcgtcag tgcctatgtc
     1801 gatgaagaaa tttcgattgg tgacttcgtt ttgaccggtg gagaaatcgc cgcgatggcg
     1861 atcgccgatt cgctcatccg cctccaaaaa ggcgccatca ccagttcatc gctcgatgaa
     1921 gaatcttttg aaagcggcat gcttgagtat ccccagtata ccgaacctta tgactatgaa
     1981 ggcagaagag ttcctgacat tctctactcg ggaaatcacg ccgccatcag caaatggcga
     2041 aaaaagcaag ctttgtatat cactcgtctg tatcgcccgg atttattcgc tcagttaaaa
     2101 ctaggccgca gcgatataaa actcctgcac gaaatcgata aaaacaccga acccgaatgg
     2161 gaaaaaaagg ccctcgaaaa aggggctaag tttatcaaaa aatagccttt aaaaacctaa
     2221 gccgccaaag ccgcctaaac caccgggcgg cattttattt cctttttgca tctgccccat
     2281 cttgcgcatc atctcttttg atttttcata cttgttgatg atgcgattaa tatcggcatt
     2341 tgttttgccg ctgccgcggg cgatgcgcac ttttcgcgaa ttgcgaagaa tttcgggatg
     2401 gcgtctttct tcggcggtca tcgaattata aatggcttca aaattggcga tttctctttc
     2461 ggcctgcgct ttttgttcat cgcttaattt gggcatgccg ggaatcatgc tcatcaagcc
     2521 gccgagagaa ccgagcttct gaatttgctt catctgactc agcatgtctt caagatcaaa
     2581 ttttcccgac atcatgcggt tgacggattt cttcatctcg cgctcatcga gttcttcctg
     2641 aactttctcg acgaggctga caacatcacc catccccaag atgcggtcgg ccatgcggtc
     2701 ggcatggaaa acatcgatat cgatgagttt ttctccggtt cccgagaact ttatcggaac
     2761 gccggtcagg tggcgaatcg acaaggcggc tccaccgcga gcgtcaccat cgagtttggt
     2821 catgacggcg ccggtcagct ttagacgggc attaaaagaa gaggcgacat tagcgacgtc
     2881 ctgaccgctc atcgcatcaa aagcgagaag aatttcatcc ggttcaactt cgccgataat
     2941 atcgttcagc tccttcatca ggggctcgtc gagatggagg cggccggccg tatcaataat
     3001 gaggacatca taatggtcgt taaatgcttt caaacgcgct tttttggcga tttcgacagg
     3061 acttaacttg tttctttcag cgatgatatc gacggcgata tcgccggcga tttgctcgag
     3121 ttgatcgatg gccgccggac gataagtgtc gcatgctgcc aacaagactt ttttattcat
     3181 tttgcccttc attaaaagag cgagttttcc agccgtcgtc gtctttcccg aaccctgaag
     3241 accaactagc atgatcagcg tcggcttgtt gcggttaaag gtgagctgat catcgctgga
     3301 acccagcaat ttctcgattt catcatggac gattttaacg atcgtttgcg aggggtttaa
     3361 tttggttaac acctcttcgc caatcgcccg ctttttgact tcggcgagga agtctttgac
     3421 gactttaaaa ttgacgtcgg cttcaaggag ggcgacgcga atttccttga gcatgtcatc
     3481 catattgctc tcggtcaagc gcgactggcc gcgcagtttt ttaaagatgc gagcaaatct
     3541 ttcgctcagc gactcaaatg ccatgtttaa tttttccttc taattcttca agggccgcga
     3601 ggacttgccc gcgacttttt tctttgcttt ctttaaagag tttgacgagg ccgagttttc
     3661 cttcgaattt ttcgagtttc ttcgcagttt tggcgattgt atcaagaacg gcactgcgcg
     3721 aaatcttgcg attctcacta atttcgccga gcgacaagtc aaagagataa taatcgctca
     3781 taatctgctg ctgtgaagtc gttaaaaggc ggctataaaa ctccatcagt tcactatatt
     3841 tttttgttcg ttcgaggtta tctttcatcg ttcgtctcca agacataatc cgtagaggta
     3901 ttcgtcgagg tcaaattcct gcaagtcatc catcttttcg ccgaggccga taaagcgcac
     3961 cggaagggcg agttcatcgc gaatcgccag gatgattccg cctttggatg ttccgtccat
     4021 ctttgtgatg acgatacccg aaaccggcgt cgcttcttta aaagctttag cctgcaacac
     4081 gccgttttgg ccggttgtcg catcgatgac gaggaacgtc tcgtgagggg caccggcaat
     4141 ttctttggcg agaacgcgcg atatctttcc gagttcggcc atgagattca gctttgtctg
     4201 aaggcgtccc gccgtatcga cgattaaaag gtcaatctta tctttgatcg ccctttgaga
     4261 cgcttcataa gcgacgctgg cgggatcctg agctggcttt ccgataacaa tcggacaatt
     4321 gaggcgtttg gcccatactt cgagttgttc ggtcgccccg gcgcgaaagg tatctccggc
     4381 tgcaagcatc acttttttgc cttgcgatag gtagcgatgg gcgattttag cgatcgttgt
     4441 cgttttgccg acgccattga cgccgaccat cagcatgacg gtcggaacat ttttttgaaa
     4501 ttgaatttca ttttcgatgt cgccgccttt agtggcatag ctgacaaata aggcatcaac
     4561 taacaattca ttgataactg atgggtcgct tatctttttt tctcgggcct ctttaagcag
     4621 attttcaatc gtccgaatcg tcagagcaat cccgacatcg gcctcgatta aaatcgtctc
     4681 aagctcttca aaatatgcct cgttaaccgc tgcataacgc ttggtgagaa ctttaagccg
     4741 atcggcgaag tgatgccgcg acttttctag accgctgaca tattttgtcg ttttctcgct
     4801 tttgcgcgaa aacttatctt ttagataagt aaaaagaccc attatttgct tcccgctttt
     4861 ttctcaagag catttttggc cgcttcttgc tcggcggctt ttttgctgga tccggttccc
     4921 cggccgagaa cgacaccgcc gtcgaggacg acttccatct cgaaaacgcg gttatgggcg
     4981 gggccatcaa cactcacctg acggaaggtc acggaatggc gatgttcgag ttgcgtctct
     5041 tcctgaagcc gcgatttgta atcgattgtc ttctcgggcg tgtaatttaa aatatcgtgt
     5101 tcaaagacgc gtttgacaat gccgtatgcg accttaaatc cgaggtcgag atagacggcg
     5161 ccgataaatg attcgaagac attttcgagc attttgtcgg acttggccac ttcaccgcta
     5221 aaactattgc cgacgacgat aaactcggcg aggtggtgat agcgggcgta tttggcgagc
     5281 gctttggtat tgaccaatga gctgcgaagt tttgtaagaa aaccctggtc tttatccgga
     5341 tgagcctgaa aggcgaggtc agcgaccgaa gcgccgagaa tcgaatcgcc gagaaactcg
     5401 agtcgctcat agtcttcgtg acaacttttg gcgtcgagat tatatgaggg gtgcgtaaac
     5461 gccaattcat aagttctgag ttcttttggt tttaaatgaa aatatttaaa taatttagct
     5521 aagcggtgca ttttctttga ttcctttttc gatgagggcg gggacatcgg cttgggcgag
     5581 gcgataagcg acgcgcaggg cgtgcatgaa agcgaggcca tcgctcgagc catgagcttt
     5641 gacggccact ttgcttaaac cgagaaacat cgcgcctccg tagtttttgt aattcatggc
     5701 ttctgacatg cgctcaaagc ccttttttga taaaagatag ccgatttttg aaaagatatt
     5761 gcgcataaag gcgcctttaa taaaaccgga catcatcttg gccacaccct cacatgactt
     5821 taaaaagaca ttgccggtaa agccatcggt gacgacgacg tcggcggtgc cattaagagc
     5881 atcgcgagct tcgatgttac ccatgaagcc gggaaaagaa ccggccaata actgataggc
     5941 atcctttgat tcgggtgagc ctttttcggg ctctgaacca ttggagagaa gatagacgcg
     6001 gggttctttg acttcgagga cggcctgagc gtaaagcttg cccatcagcg cgaactggac
     6061 gagttcggaa gcggagtttt cattggaagc tccaatgtcg agaatgacga cttgctgacc
     6121 gaggtttttg gtcggaaacg gggagatgag agcggggcgc aaaacgcctg gcaacttctt
     6181 gaggcggatc gatgttgaac caagataggc tccggtgctt cccgacgaga cgagggcgtc
     6241 ggcattttga gccaaaactt ggtcgatggc aatcgccatt gaggcgccct tcgcccgaat
     6301 ggcatccatg acgccggctt ccataggaag aacttcgcgg gcatccacta aagtagcctt
     6361 accttgaagc aaggtcaact cttcaatttt accgacggcc gtcacttcga catcccggtg
     6421 atcttttaag aaggcgagaa cgccttcgac ggcggccgcg cttccgaggt caccgcccat
     6481 catatcaata acaattttaa tcataaatat tccttctttg ctattttaac atttagcatc
     6541 gtttatctaa ataaaaacga tgcgcacgag gattagacgt ttgtaaattt ggcctgggcg
     6601 atttctcttt tggctcttgc gatgatgcgt cggtaggagg tctcaaaagg gcgttccaga
     6661 attgttttag cgtcgttgcg ggcgtattcg aacatcttga aatcatcgat gagattaacg
     6721 aattcgaaat tggtgatgcc ggattggcgg agcccgctca gctcacccgg tccgcgcctt
     6781 cttaaatctt cctcggcgat aaagaagcca tcatttgatt cgacgagaac atttaacttg
     6841 tttatttcat cttcatcctc gccatcgtag acgagcagac agatcgcttc tttgccatcg
     6901 cggccgatgc gcccccgcag ctgatgtagg cttgctaaac caaacgatgt gggagcatag
     6961 ataaccatcg ctgaagcctc tttgacatcg atgccgactt cgatgacgct ggtcgagaca
     7021 ataatcggcg tcttgccgct gataaactcg ccgagagcgt aattcttgtc gtcgatgctc
     7081 atctgcccgt ggaggaggct gacctgcccg ggatagagct tttcgtaata cttgaatagc
     7141 tcttcgacgg agacgaggcc ggtcgaactg atatcgatga cgggagcgac gacatagacg
     7201 cgctgctgtt tgttaatcgc ttgcttgatc gtcttatgaa tcagcgaatc gctgtttgag
     7261 acgactttgg tcatgatctt tctttggctg aaggggaact gagtgagcgt cgagacatcc
     7321 aagtcgccgt agagcgtcag agctaacgtg cgtggaattg gtgtggccga catgaggagc
     7381 aaatcggcgt gttcgccttt ggatgcgaga aggaggcgct gattgacgcc gaagcgatgc
     7441 tgctcgtcaa ttacggcaag accaagcgag gaaaaagtaa catcctctga gaagagggcg
     7501 tgggttccca ccaccacatc aatcttatga ttgataagac ccatattgat ttgaaaacgc
     7561 tcttttttgg gtgtggctcc caaaagcaag gcaaggcgca ccggcgtatc tttgaaaagt
     7621 tcgcgtaaag tgcgaaagtg ctgcctggcc agggcgtcgg tcggagccat caaagcgcct
     7681 tgatcattgc ggaggaaatt gccatagaag gccagggcgg ccactaacgt tttgccggtt
     7741 ccgacatcac cttgcaaaag gcgatacatc aagcttcttt gatccatgtc tttgacgatt
     7801 tcggcgaccg aactaatctg atcttcggtg agtttatagg ggagggaccg aatgaaggtt
     7861 tttaatttgg tgcggtcgat aaattgcttt tcatttttaa tgagttcttt attttgcgag
     7921 cggataatct gtgtttttaa cgaaaagagc agacactcct cgtattttaa aacgcggagc
     7981 ccctcataaa tatctttagc tgaaaacggg cgatggacga gatggagggc tttttcttta
     8041 ttcaagagtc ggtatttttc ttgtagaggc gctggtatgt cgctttcaag cgcttcttct
     8101 ttgccatcaa aagctttttc cacgaggtga gaaaactggt aattcgtgat cgcgcgggga
     8161 agcgtgtaaa ccgggcgcag ataatcgccg gcgggcggtt cacctttggt gagattaatc
     8221 atattgagtt tatttttgta gtaatcgtag ttagcgacta aggtatagac gctttcacta
     8281 tcgagcatct tggttagata ggggcggttc caggcttcga cggcaaaggt gtttccggcc
     8341 ttggttgtaa aagaaaagcg ggtgacctgg gctcgtgaaa agcgggagtt gaccggcggc
     8401 cttgtcacgc ggccgaaaaa gacgacgcgc tctttgtctc tgaggccaaa ttcgcgggtt
     8461 ggctcgagat tatcatactt gcggggtaga tgaaaaataa tgtcttcaaa gctatataaa
     8521 cccatgtcat aaaggacttt atttatgcgc ggagacttcg aaaatttcat agtcatattt
     8581 taccaaattc atgggctgat atcaaaaaaa gaccgatatc ggtcttattt cgtttttatt
     8641 cgacgccaat caggaaggag tagacaggtt gctggccgcg cttgatgtcg atttcaagtt
     8701 cgggatatgc ttcaaagagc gattcgctca atttcgattc ctcgctctcg gtgacatctt
     8761 cgccgaggat gacggttaga atcgccgcat tttcatcgat catcgctttc aagagttcgt
     8821 gcaacgcttc gatcttgtct ttgacgcaga cgatgatcga tttattgcga aagcccatgt
     8881 agtaaccttt agtgacatga ataccttcga tgtccgtgtc tttgatcgca taggtaaccg
     8941 aaccggatac gacattttta agggccgctt tcatctcttt aaaattttct tcgggatcga
     9001 gatcaggatt aaacatcatc gcagcgacaa taccttgtgg tattgttttg ctcggaacca
     9061 cgcgggcgat cgtctcgcct tcgatgacgt cgcaagcttg ggaagcggtc atgacgatgt
     9121 tggaattatt gggaaggatg tagacgtttc gggcgtgaac cttttcgatg gctgtcaaaa
     9181 aatcttcggt cgcagggttc atcgtctgcc cgccgctgac gatgtaatcg acgccgatat
     9241 cggaaaagat ctgattgaga ccatcgccgg cgctcaccgc gattaaagcg taatccttaa
     9301 gcggtttttc ttcgactgcg ggttttgctt cttcgcctgg cacgatgtcg agatggctgt
     9361 gctgttcggt catgttttca atttttaaag tgacgaattc gccaaaattt tgcgcgtaat
     9421 ttaaaatcga accgggttcg agggtgtgaa cgtgaacttt gacgatatca tcgtctttga
     9481 cgacgacgag cgagttgccg tggctattca agacgccggt gaagcgcttt tcgacaaacg
     9541 gctttttctc ggcgtccgga ccgaggcgaa ggataaattc cgtacagtaa ccgaattcgt
     9601 cttcgctccg ctcgccttta aactccggct gcggcattct ttcctcgaag acagtggcct
     9661 gcgatttctc gacgacatcg ccgagcaatg ctctttccat cccttcaaaa atggtgacga
     9721 gaccaaagcc gccgctatcg acgacgccga cttccttcaa aatcggtaag agctccggtg
     9781 tcctttgcag tgaagcttta gcttcattta ataaaaaggc aaatgctttt tcgatggcaa
     9841 aatcgctgtt gacatgcgcg agcatagctt tcgaagactc gcggatgacc gttaaaatcg
     9901 tgccctcgac ggggcggata acggctttat aggcgacttc gctgccgttg acaaaagctt
     9961 cggcgagatc gacggcattc atgactttct tgccttcgag cgactgggca aaaccacgga
    10021 aaatctggct ggtgataacg ccggaattgc cgcgcgcgcc catcaggagg ccttttgaaa
    10081 aggcgaccgc gatcggataa acttcgctgt cgttgcgatt ttgaatttcc ttcatgcctg
    10141 acgacatcgt cagattcata ttcatgccgg tatcgccatc cggcaccgga aagacgttga
    10201 gggagtcgac ttcaggatag tgattataaa gattattcgt tcccgaaata atcatctgtt
    10261 ttaataattg gccatcgata tttttcattc tttttttccg ttaattaatt gttttaagac
    10321 tctggacata aacattgatc gccttaaagt ttaaatcaaa cgttttttct aaaacgtatt
    10381 tgatgcgctt ttgcacttgt gagacgatct cggtaatttt cacgccgtaa gcgacgacga
    10441 tgtagacgtc gacttcatag gcatccttgc tctttatcac ctgcacgcct ttcgcgtacg
    10501 aatcggattt caaaagttcg gcgatgtttt cgcgaatcga ggatttgctg gctaagccga
    10561 caacaccata gcattcaatc gcggcgtttc cggcaattgt ggcaatggct tccgtggata
    10621 tgtttatgtc gccatacggc gttgatttat caattgtcat tggttttaag gcaaaggtaa
    10681 atattacctt ttaagaaata tagcacatgt aaaaaataat tccaagttat atatcttcac
    10741 gctcctagat agtatcgttc ttcgtccgtc agttctcaaa ttagttcaag gtttggatcc
    10801 agagctttta aagctgttcc aagtcgttgc ttatgatgcc caaacatgat gagatggtgg
    10861 tttcctaatg ggtggcgaag aattttttcg agtccgctat tcatctttac gacaatctgc
    10921 gtccggcaga gattctcaag cgccaagtta gcgactatct cgccttcttc aatcagggcc
    10981 ttgctgaggt ttcccgagag gcgaaagacg gtgcacggtc caagcggcaa ttcaccgcgc
    11041 acgccgatgc cgagacctga ttcaaaatgg gtcatgaaaa catggctttg gcacattgaa
    11101 aacggcacgg tgcaatgtgc gaaagtaatt gtttgtttcg cctcgtcaat gctgctcgga
    11161 ttggcctgaa acgaatcaag agacagcaat cttttgacca agtgcatagc gagtaaaagc
    11221 gggagatcac cttcgcaacc agcgctgatt ccttcgttgt ttagcatggc gagggccaaa
    11281 cacgcggtat tgcgataaat agttaataaa tcaaagcagc gaatactaaa accgtgtaaa
    11341 tcatacttta aaatcagcct ttttatcgcg ccgtaaatat gaagagcgcc ttctaaatac
    11401 acgttattag caagcttttt tctgatttgc cgcacttttt cgtcatctgc ataagatttc
    11461 ttgtcgattt caccaacgaa ttcgtcataa tcaatgttaa tataagcgac tttatatctt
    11521 ttgtaagcca aatcccgatt gaggtcggag gcgat
//
LOCUS       DAOSSL010000028        11453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5585_length_11453_cov_15.685296, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000028 DAOSSL010000000
VERSION     DAOSSL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5585_length_11453_cov_15.685296"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1934)
                     /locus_tag="PLH17_03560"
     CDS             complement(18..1934)
                     /locus_tag="PLH17_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOR20880.1"
                     /translation="MAIDKHRNDTAISYIDDKNRIVRYNYETYRTITFKLASKLDIVL
                     ANITKKEIVALKMKNSPTWIHLFWALLSCGYRVLLIDARLPKANTDLLLKQANAKAIV
                     CDEIHEYSVPRFTISEIEEKEANYKFAEMWANEVIFCSSGTTGEAKMMVFNGNNLCHQ
                     IYAAYGMPETTKDIMHPGPINIMALVPFHHIFGFVAVFLWYTFFGKNIIFIKTLSPAE
                     VLTTAQRLSATHIYCVPLFWDSLAQNIVRKVALEGPKKVALLDRMIAYNTHKISAFEA
                     GLAATPIALAAVQKNIFGSKVKYCISGGGYISEKTMNIINGIGYPLYNGYGMTEVGVT
                     SVELSPRVEDRLKSSIGRPFEGVTYKIAPTDEKTSGVNLGELLIKSNITHVREIVGGV
                     EKAPDLEDGFLRSGDIVFRDGSGNYYIKGRIKDIIINANGENVYPDEIESYFKSIPHV
                     SNTCVVGVRKGKSSDETITLILEVDNEISTEEFALLKDKVQEINVGLSNEKRVQDVFV
                     IKDKLPISNNLKVRRFQVKDLLKAKPEFFLDMNEKKAVKTFDGFESSVVEPIVKSVRE
                     IFSKVLLLPTFKVGDSEHWVNELGGDSMSYVQLVGEIEDYFGIKIAEEKYGLLVSVND
                     FAEMVLTYRGKKSK"
     gene            complement(2086..2586)
                     /locus_tag="PLH17_03565"
     CDS             complement(2086..2586)
                     /locus_tag="PLH17_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOR20881.1"
                     /translation="MKTKKEKPEGYYASIKRRDPAARHNFQIFFLYPSVIALRYYRLA
                     HWFWTKLHLKFIGEWIMCMVKCRTGIEIHPAATIGRNLFIDHGSGVVIGETTIIGDNV
                     TLYHQVTLGGVSSKKVKRHPTIGNNVVIGAGAKVLGNITVGDNAKIGVNAVVRADVPS
                     GVKIIK"
     gene            complement(2576..3469)
                     /locus_tag="PLH17_03570"
     CDS             complement(2576..3469)
                     /locus_tag="PLH17_03570"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOR20882.1"
                     /translation="MIYNHITELIGNTPHLQLPSINNNQLYVKLEMFNPGGSVKDRIA
                     LSMVNDLVERGLLNGASKVVEATSGNTGIGLSFVLASKGISFTAVMPENMSRERVDLF
                     RAYGTHIILTPAALGMTGAIAKAKEMEQEGYVYIDQFRNHANPEAHMKTTAEEILRDF
                     PKLDYLVLGVGTGGTLTGLARIIRKRYPNLKIIAVEPFESSVLQGLPKGPHKIQGIGA
                     GFVPPLLETKEVDRIISIASADAMNKAKLLARQGLFLGISSAAAILASEIVADQVKNA
                     VILTVAPDGGAKYISTGAYEN"
     gene            3548..4483
                     /gene="fmt"
                     /locus_tag="PLH17_03575"
     CDS             3548..4483
                     /gene="fmt"
                     /locus_tag="PLH17_03575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOR20883.1"
                     /translation="MNKKDYRLIFMGTPEIAAFVFESMIQDGYHFVGLIAQSDSPQGR
                     KNILTKVPTKIVAEKYGIPVFQPEKIRLDYEFVKALKPDLIVTFAYGQIIPQGLLDIP
                     RLGCLNLHGSILPKYRGAAPIQRAIMAGETKTGITLMEMIDKMDAGKMYARAYVEILP
                     DDNHSTLSLKMARAAAQLIREKLPLYLLGQLPGQAQDESLVTFANKIKPEDEKIALSL
                     SVVACVNYIRALSEAPGAFLLLNGQKLKIYQAAYHSSDVGGELGAIVDTRGGVIVQLH
                     DGQIALLDVQLEGRKRLKYKDFINGQKNLIGSVLS"
     gene            4485..6824
                     /locus_tag="PLH17_03580"
     CDS             4485..6824
                     /locus_tag="PLH17_03580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOR20884.1"
                     /translation="MDNRLKLSVHQLVDFVLRTGDIDNRIFNRSSMNEGTRIHAFYQS
                     KQGVNYLSEYLLGGTFYNSGYTIFLEGRADGIIIDGAFAIIDEIKSTVVELEDYHASQ
                     EAWHVGQAQCYALMYALEKNLDRVGVRLTYIHQSKDEKLIKNYVFSRAELEEKVASYL
                     ESYLAFYAIIMRRIEKRNETAGTLSFPFLNFRKGQRELAKYAYGIAKNGGTLFAEAPT
                     GIGKTISTLYPFVRSFSDGVNEKIFYLTAKNSGKEAALQAVELMKSKGVKLSEVLVTA
                     KDKICFCPGKACNPDECPFAKDYYTKIRDVLTKSLARYDTFDSSRVSRIAAAHHICPF
                     ELQLDLSLYVDVIICDYNYLFDPLVYFRRYFDNDASRMLALIDEAHNLVDRGRDMYSA
                     SLDSASFEKARKAVRKLDHKKYKNASKRIAKLFNELKENSPIGETVLANFDAKIIRAM
                     EAYLLATQDINRNHHDHVDEDFRQFFLDINRFLKIYEFVDDSFTLYVTNTNDKFVKIS
                     IFCLDPSRHLARSLGQVKGRILFSATLSPHEYYVDVIGGRKEDPVLLLPSPFEADNLL
                     LMIAPTVSIKYKNRADTYKVVAEYIENFVSHKMGNYLIYAPSHAYLAELVEHLNFPDD
                     YDVFVQLREMDDLDKERFLERFQLNPSKTTIGIVVVGGVFGEGIDLVEDRLIGVAVIG
                     VGLPQLGFERDLIRDYFDKRGQKGYEYAYVNPGMNRVMQAVGRVIRSEKDRGMALLID
                     DRFLNARYRDLFKHEWSHYDVVISPEDMALSITSFWKKA"
     gene            6867..8675
                     /gene="lepA"
                     /locus_tag="PLH17_03585"
     CDS             6867..8675
                     /gene="lepA"
                     /locus_tag="PLH17_03585"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOR20885.1"
                     /translation="MTYDQSRIRNFSIIAHIDHGKSTLADRILELTNTVEKRQMKEQL
                     LDNMDLERERGITIKLNAVSLKYKADDGITYDFNLIDTPGHVDFTYEVSRSLAACEGC
                     LLVVDATQGVEAQTLANVYLAVDNDLTIVPVINKIDLPSAMPEVALDEIKNRIGIPVE
                     EALLISAKTGQGIKGVLEAIVKHIPSPGGDNSAPLQALIFDSYYDSYRGVIALVRIKN
                     GQVRAGDRIRLMSTAKDYLVTEVGFHAPYEVKCEKLMTGQVGYIAAQIKDIKDIFPGD
                     TITLVDNPAKEALPGYRRINSMVFCGLYPVDSKMYLDLKDALEKLALNDSSLIFEPET
                     SQALGFGFRCGFLGLLHMDVVQERLEREYGLNLILTAPSVVYRINLTDGSTITLDNPS
                     KLPEPTLIESIEEPYVKAAIMTPKEYIGPIMELCQEKRGIYIDLEYFDDTRMILNYEL
                     PLLEIIYNFFDKLKSFTRGYASFDYSLSDYKASRLVKLNILLNGNVVDALSSIIHRDS
                     AFNRGNSIVRKLKGIIPRQQFEIPVQAAINGKVIARADVKAVRKDVLAKCYGGDISRK
                     RKLLDKQKEGKKRMKTIGSVEVPQEAFMTILSIDDE"
     gene            8759..10141
                     /locus_tag="PLH17_03590"
     CDS             8759..10141
                     /locus_tag="PLH17_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOR20886.1"
                     /translation="MANSDLSVRFTDETYATRSDVMRALNTSLIDSIWRNIEEYRSRF
                     SRIISLRDIAKRPFRVTFTPNITDKITGLERKFARAMSQFSQLDRNDSERVAIQKESY
                     RKILAYLAQKNNIVATDVDIMAIVTDQNFNSAHQGLVDYYKTLRHFEQYHHDPIDDNF
                     LADFLARFKGEDELVSFYREKDYARPYQTVIGKEYDFAPAATIEFLMNDLFDFINNSE
                     ISFVVKAVAAYYFIDYVKPFENHNEEVAIILAKSILAHNDIGDVSAFLPLEALFAMRN
                     ENFRKIFLEVQKTNDLTYALLAVIDLVLNEVQNFLDLITQVKMTEVKKEFRSLGSELP
                     AAKPASEPKPEPKAVVKEEPAVASKGEKVRPAEVVAVPQPLELPKEEDVKSIIKNLLE
                     SNPLLRPKQAEFYARHRTLGKYYTISMYKKEMDVVYETARTSMDNLAQLGYYRRENVK
                     NKFVYTPIKK"
     gene            10157..10444
                     /locus_tag="PLH17_03595"
     CDS             10157..10444
                     /locus_tag="PLH17_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20887.1"
                     /translation="MESLFVVLASAGWETPVILFSLLGFFGLVVLGVIFAKRHIKGLQ
                     SKDDQPIDEEKAIEEELKRVLEPIEDEKIQEEMAKAKHEKEPDADKDKSQG"
     gene            10401..>11453
                     /gene="hemW"
                     /locus_tag="PLH17_03600"
     CDS             10401..>11453
                     /gene="hemW"
                     /locus_tag="PLH17_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOR20888.1"
                     /translation="MRKNPMPTKISPKANSLYIHIPFCHHLCHYCDFPKLLYDEKMAF
                     SYLDQLCKELDSYHIDEVATIYVGGGTPSALPDALFDKLLDKIAPLLKEDGEFSVELN
                     AESTTQEKLLIMKHRGVNRLSIGVQSTDDQILASLNRKHSFEDVKKIVKLARAVGFNN
                     INLDLIYGVPSQSENILKLDLKRLLALHPEHLSIYSLTVHPGTIFFLKGIAEQDQDLS
                     RDHYDIILRELRKKGYTRYEVSNFARPGFFSLHNQTYWHNEEYYGVGLGASGFVGGVR
                     YDNTRNLSKYLAGAYRQSEEHLTLEDDEKYFWMLNLRLESGFAIKDYIKRYGEEMLNA
                     RLYLLQDAIKNGLMIKS"
BASE COUNT         2954 a         2828 c         2741 g         2930 t
ORIGIN      
        1 gttattcgtt ccgctcgtta tttgctcttc ttgccgcggt aagtcaagac catctcggca
       61 aaatcgttga ccgagacgag caagccgtat ttttcttcgg cgattttgat tccgaaataa
      121 tcttcgatct cgccgacgag ttggacatag ctcatcgaat cgccaccgag ttcattgacc
      181 cagtgctcgc tgtcgccgac tttaaaggtc ggaaggagca aaactttcga gaatatttcg
      241 cggaccgatt tgacgatcgg ttcgacgacg cttgattcga agccatcaaa ggtcttgacg
      301 gccttcttct cattcatgtc gaggaagaat tccggtttag ctttgagcag gtctttgact
      361 tgaaagcggc ggactttgag attgttggaa atcggaagct tatccttgat gacaaagaca
      421 tcctggacgc gtttctcatt agatagtccg acgttgattt cctgaacttt gtcttttaaa
      481 agcgcgaatt cctcagtcga tatctcatta tcgacttcga ggattaaggt tatcgtctcg
      541 tccgaggact tgcccttgcg gacgccgacg acacaagtgt tgctgacatg gggaatcgat
      601 ttaaagtaag attcgatttc atcgggatag acattttcgc cgttggcgtt gatgatgata
      661 tcttttatcc ggcccttgat gtagtaattg ccgcttccat cgcgaaagac gatgtcgccg
      721 cttcttaaga agccgtcttc gagatccggg gctttttcga cgccgccgac aatctcgcgg
      781 acgtgtgtga tgttcgattt gataagcagc tcgccgaggt tgactccgct cgtcttctca
      841 tcggtggggg cgattttata agtgacgcct tcaaacgggc ggccgatcga actcttcagg
      901 cggtcttcga cgcgcggcga gagctcgacg ctggtgacgc cgacttcggt catgccatag
      961 ccattataga ggggatagcc gatgccattg atgatgttca tcgtcttttc ggagatgtag
     1021 cctccgcccg agatgcagta tttgactttg gagccaaaga tatttttctg aacggccgcc
     1081 agggcgattg gagtggcggc taaaccggct tcaaaagccg aaatcttgtg ggtattgtac
     1141 gcgatcatgc gatcgagcaa ggcgactttt tttggtccct ccagggcgac tttacggacg
     1201 atattctgcg cgagcgaatc ccagaataag gggacgcagt agatgtgcgt ggcgcttagt
     1261 ctctgggctg tcgtcagcac ttcggccgga gacagagtct tgataaagat gatgtttttg
     1321 ccaaagaagg tataccacaa aaagacggct acaaagccga agatgtggtg gaatggcaca
     1381 agtgccataa tattgatcgg acccggatgc ataatatctt ttgtcgtctc gggcatgcca
     1441 taggcggcgt agatctggtg gcataagtta ttgccgttaa aaaccatcat cttggcttcg
     1501 ccggtcgtcc cgctcgagca gaagatgact tcgttagccc acatttcggc gaatttatag
     1561 ttggcttctt tttcttcgat ttcgctgatc gtaaagcgcg ggacgctgta ttcgtgaatc
     1621 tcatcgcaga cgatagcttt ggcgttggcc tgcttgagaa gcaagtcggt gttggctttg
     1681 gggaggcggg catcaatcaa taagacgcga taaccgcagc ttaaaagcgc ccagaacaga
     1741 tgaatccacg tcggagaatt tttcatcttg agggcgacga tttctttttt ggttatattc
     1801 gcgaggacga tgtcgagttt tgaagctaat ttaaaggtga tggtccggta cgtttcgtag
     1861 ttatagcgaa cgatgcggtt tttatcgtcg atataagaga tggccgtgtc attgcggtgc
     1921 ttgtcgatcg ccattaaaaa aatatcttgc atcgttcgcg cggtcttcaa gaggtcggct
     1981 accgaggcaa aataccgtct tatcttatta taatcagaat acattgctta tgctcctttc
     2041 gcatacaaca caatatattc taaaagaatt ataaattaaa gtatactact ttatgatttt
     2101 gacacctgat ggtacatcgg cacgaacgac ggcgttgacg ccaattttgg cattatcccc
     2161 tactgttata tttcctaata ctttggcacc ggctccgatg acgacattat tgccaatcgt
     2221 cggatggcgc ttgacttttt tcgacgagac accgcccaaa gtcacctgat ggtaaagggt
     2281 gacgttatcg ccgataatcg tcgtttcgcc gatgacgaca ccggaaccgt gatcgatgaa
     2341 tagattgcgg ccgatggtcg cggccggatg aatttcgatg ccggtgcggc acttgaccat
     2401 gcacatgatc cactcgccga taaacttaag atggagcttg gtccaaaacc agtgagcgag
     2461 gcgatagtat cgaagggcaa tgaccgaagg ataaaggaaa aagatttgaa agttgtggcg
     2521 cgccgccgga tcgcggcgct tgatcgaggc ataatagccc tcgggctttt cttttttagt
     2581 tttcataggc gcctgtcgag atgtatttgg ccccgccatc gggagccact gtcagtataa
     2641 cagcattttt gacttggtcg gcaactattt cgctggctaa gatggccgcg gcgctcgaga
     2701 tgcccagaaa gagaccctgc cgcgccaata acttagcttt gttcatggca tcggcgctgg
     2761 cgatcgagat gatgcgatcg acttctttgg tctcgagaag cggtggaaca aaaccggcgc
     2821 cgattccctg tattttgtga ggtcctttgg gaagaccttg caaaacgctc gattcgaatg
     2881 gttcgacggc gatgattttt aaattgggat agcgtttgcg gatgatgcgg gccaggccgg
     2941 tcagagtgcc gcccgttccg acgccgagga cgagataatc aagtttggga aagtcgcgca
     3001 gtatttcttc cgccgtcgtc ttcatgtggg cttcgggatt ggcgtgattg cggaactggt
     3061 cgatgtagac atagccttcc tgctccatct ctttggcttt ggcgatggct cccgtcatgc
     3121 cgagggcggc cggcgtcagg atgatatgag tgccataggc gcgaaagaga tcgacgcgct
     3181 ccctggacat gttttcgggc atgaccgccg taaacgagat gcctttgctg gccaggacga
     3241 atgataatcc gatgccggtg ttgcccgagg tcgcttcgac gaccttgctc gcgccgttta
     3301 aaaggccgcg ttccaccaga tcgttgacca tgcttaaagc gatgcggtct ttgacggaac
     3361 cgccggggtt gaacatctcg agtttgacat agagttgatt gttgtttatc gatggcaatt
     3421 gcaggtgcgg cgtattgccg attaattcgg tgatatgatt gtaaatcatt gtgaaggtct
     3481 ccttagttgt ctataataat atattagttg atttagtata acaaacgaaa cgatatgagg
     3541 tgctttcatg aataaaaaag attaccgact gatatttatg gggacgcccg aaatcgccgc
     3601 cttcgtcttt gaaagcatga ttcaggacgg ctatcatttt gtcggtttga tcgcccagag
     3661 cgattcgcca cagggtcgca aaaacatctt gacgaaagtc ccgacaaaaa tcgtcgccga
     3721 gaaatacggc attcccgtct ttcagcccga gaaaatccgc cttgactacg aatttgtcaa
     3781 agcgttaaaa cccgatttaa tcgtcacctt tgcctatggc cagatcattc cgcagggatt
     3841 gcttgatatt ccgcgtttag gctgcctgaa tctccatggt tcaatcctgc ctaaatatcg
     3901 cggcgcggcc cccattcaaa gagcgattat ggccggtgaa accaagaccg gcatcacctt
     3961 gatggagatg attgacaaaa tggacgccgg caagatgtat gctagagcat atgttgaaat
     4021 tttgcccgat gataatcact cgactctatc attaaaaatg gctagggcag ccgcgcagtt
     4081 gattcgcgaa aaattgcctc tttatcttct cggtcagcta cccggtcagg cgcaagacga
     4141 gagcctggtg acatttgcca ataaaatcaa gcctgaagat gagaaaattg ctttaagtct
     4201 ctcggtcgtc gcttgtgtta attacattcg cgccttaagt gaagcgccgg gcgcctttct
     4261 tttgctcaat ggacaaaagt taaaaatata ccaagccgct tatcattcat cggatgtcgg
     4321 cggcgaactc ggcgcgattg tcgacactag aggtggcgtc atcgttcagc ttcacgatgg
     4381 gcagatcgct ttgctcgacg tccagcttga agggcgcaag cgactgaaat acaaggattt
     4441 tattaacggc cagaaaaatc ttatcgggtc agttttaagt taatatggat aaccgcctca
     4501 agctttccgt ccatcaacta gttgattttg tcttgcggac cggcgatatc gacaaccgca
     4561 tcttcaatcg cagctcgatg aatgaaggca cgcgcattca cgccttttat cagagcaaac
     4621 aaggcgtaaa ttatctatcg gaatacctgc tgggcggcac cttttacaac agcggttaca
     4681 ctattttcct cgaaggccgg gctgacggca tcatcatcga cggcgctttt gccatcattg
     4741 atgagattaa atcgacggtg gtggaactcg aggattatca cgcctcgcaa gaggcctggc
     4801 acgtcgggca ggcgcagtgc tatgccctga tgtacgccct agaaaaaaat cttgatagag
     4861 tcggcgtgcg ccttacttat attcatcaaa gcaaagatga aaagctaatc aaaaactatg
     4921 ttttcagccg ggccgaactc gaagaaaaag tcgcttctta tctcgaatca tacctggctt
     4981 tttatgcgat tatcatgcgc cgcatcgaaa agcgcaatga gacagccggc acgctcagtt
     5041 tccccttttt aaactttcgc aaggggcaga gggaactggc caaatatgcc tatggcatcg
     5101 ccaagaacgg cggcactctc ttcgccgagg ctccaaccgg tatcggcaag acgatttcga
     5161 cactttatcc tttcgtccgt tcatttagcg atggcgtcaa tgagaaaatc ttttatctga
     5221 cggctaaaaa ctccggcaag gaagcggcct tacaagctgt cgaactgatg aaatcaaaag
     5281 gcgtcaaact gagcgaagtg ctcgtcacgg ccaaagacaa aatatgcttt tgcccaggca
     5341 aagcctgcaa tcccgatgag tgcccttttg ccaaggatta ctacacaaaa atacgcgatg
     5401 ttctgacgaa gtcactggcc cgctacgata cctttgattc atcaagggtg agccggattg
     5461 ccgccgctca tcacatctgc cctttcgaat tgcagctcga cctctcgctc tatgtcgacg
     5521 tcatcatctg cgattacaac tatctgttcg atcctctcgt ctattttcgc cgctacttcg
     5581 ataacgacgc ctcacgcatg ctcgcgctta ttgatgaagc tcacaatctc gtcgaccgcg
     5641 gccgcgatat gtatagcgcc tcccttgatt cagcctcttt tgaaaaagcc cgcaaagcgg
     5701 ttcgtaaact cgatcataaa aaatataaaa acgccagcaa gcgcatcgcc aaactcttca
     5761 atgaattaaa agaaaatagc ccgatcggcg agacggtgct agctaacttc gatgccaaga
     5821 ttatccgggc gatggaggct tatctcctcg ccacgcagga tatcaaccgc aatcatcacg
     5881 atcatgtcga cgaggatttc cggcagtttt tcctcgatat caaccgcttt ctaaaaattt
     5941 acgaattcgt cgatgatagt tttaccctct atgtcacaaa cactaacgat aaattcgtta
     6001 agattagcat tttctgcctg gatcccagcc gccatctggc gcgcagttta gggcaggtca
     6061 aagggcgcat cctcttttcg gcgaccttat caccccatga atactatgtc gatgtcatcg
     6121 gtggtcgcaa ggaagatccg gtcctgcttc ttccttcgcc ttttgaggct gataacctcc
     6181 tcttgatgat cgctccgacg gtatccatca agtataagaa tcgggccgat acttataaag
     6241 tggtggccga atacatcgaa aatttcgtct ctcacaaaat gggcaactat ctgatttacg
     6301 cacccagcca cgcctatttg gctgagttgg tcgagcattt aaactttccc gatgattatg
     6361 atgtctttgt ccagcttcgc gagatggatg acctcgataa agaaagattc ctcgagcgct
     6421 ttcagctaaa tccgagcaag acgacaatcg gcatcgtcgt cgtcgggggc gttttcggcg
     6481 agggcatcga cttggtcgaa gaccggctga tcggcgtcgc cgtcatcggg gtcggcttgc
     6541 cgcagctcgg ctttgagcgc gatctgatcc gcgactattt cgataagcgc gggcaaaagg
     6601 gctacgaata tgcctatgtc aaccccggca tgaaccgcgt catgcaggcg gtgggccgcg
     6661 tcatccgcag cgaaaaagac cgggggatgg ctcttttgat cgacgaccgt ttcctcaacg
     6721 cccgctatcg cgacttattt aaacacgaat ggtcgcatta cgatgtcgtg atatcaccgg
     6781 aagatatggc tttaagcatc acttcttttt ggaaaaaagc atgaagtttt aaaacttctt
     6841 gctataatat cacggaagat aaaattatga cctacgatca aagccgcatt cgcaatttct
     6901 ccatcatcgc ccacatcgac cacgggaaat cgaccctcgc cgatcgcatt ttggaattga
     6961 ccaacacagt tgagaaacgg cagatgaaag agcagctcct cgacaatatg gaccttgagc
     7021 gcgagcgcgg aatcacgatt aaattaaacg ccgtctcgct caaatacaaa gccgatgatg
     7081 gcatcactta tgatttcaat ctcatcgaca cgccagggca tgtcgacttc acgtacgaag
     7141 tatctcgttc tttagcggcg tgcgaaggct gccttttagt cgtcgatgcg acgcagggcg
     7201 tcgaagcgca gacgctcgcc aacgtttatc tggccgtcga taacgatctg accatcgttc
     7261 cggtcatcaa caaaatcgac cttcctagcg ccatgccaga agtcgccctt gatgaaatca
     7321 agaatcgaat tggcattccc gtcgaagaag ccttattaat ctcggccaag accggtcagg
     7381 gcatcaaagg cgtcctcgag gccatcgtca agcacattcc atctcccggc ggcgataaca
     7441 gcgctcctct tcaggctctc atcttcgatt cttactacga ttcatatcgc ggtgtcatcg
     7501 ctctcgttcg cattaaaaac ggacaagtca gagccggcga tcgcattcgc ttgatgtcga
     7561 cggcaaagga ctatttggtc accgaggtgg gattccacgc tccttacgaa gtaaagtgcg
     7621 agaaactgat gaccggtcaa gtcggttata tcgccgccca gatcaaagac atcaaagata
     7681 ttttccccgg cgacacgatc actctcgtcg ataatccggc caaagaagct ttgccgggct
     7741 accggcgcat caactcaatg gttttctgcg gtctttatcc ggtcgacagc aaaatgtatc
     7801 tcgatttaaa ggatgcctta gaaaaattag cgcttaacga ttcatcgtta atctttgaac
     7861 cggaaacctc acaagccctc ggcttcggct ttcgctgcgg cttcctcgga cttctgcaca
     7921 tggatgtcgt tcaggaacgg ctcgagcgcg aatatggcct gaatctcatt ttaacggccc
     7981 ccagcgtcgt ctatcgcatc aatctgaccg atggttcgac catcaccctc gacaacccct
     8041 caaagctccc ggaaccaact ttaatcgaat cgatcgagga accatatgtc aaagccgcga
     8101 ttatgacgcc gaaagagtat atcggtccga tcatggagct ctgccaggaa aagcgcggca
     8161 tatatatcga cctcgaatac ttcgacgaca cgcgcatgat tctcaactac gaacttccgt
     8221 tgctcgaaat tatttataat ttctttgata agttaaaaag ctttacgcgc ggttatgcat
     8281 catttgacta cagcctcagc gactacaaag cctcgcgcct tgtcaagttg aacattttgc
     8341 taaacggcaa tgtcgtcgat gctctatcaa gcatcatcca tcgcgattcg gcttttaacc
     8401 gcggcaactc gatcgtgcgc aagcttaagg gcatcatccc gcgccagcaa ttcgaaattc
     8461 cggtgcaggc cgccatcaac ggcaaagtca tcgcccgcgc cgatgttaaa gccgtccgca
     8521 aagatgtttt agccaagtgt tacggtggcg atatcagccg caaacgcaag cttctcgaca
     8581 aacaaaaaga aggcaagaag cggatgaaaa cgatcggtag tgtcgaagtt ccgcaagaag
     8641 cgtttatgac gatattatct atagatgacg aataatttag cataagttta gtatatattt
     8701 agtgaatttt cttgaacatt tttgctcgtc cttataagat aaaggcgagg acaacattat
     8761 ggcaaactca gatttatccg tccgcttcac cgacgagaca tatgcgacgc gcagcgatgt
     8821 catgcgcgct ttaaacacca gcctcatcga ttccatatgg cgcaacatcg aagagtatcg
     8881 cagccgcttc agccgcatca tatctcttag agatatagca aagcgtccct ttcgcgtgac
     8941 tttcacgccg aacatcactg ataaaattac gggtctagag cgaaaattcg ctcgcgccat
     9001 gagccagttc agtcagctcg accgcaacga ctccgagcgg gtagcgattc aaaaggagag
     9061 ctaccgcaag attctcgcct acctcgccca gaagaacaat atcgtggcca ccgatgtcga
     9121 tatcatggcc atcgtcaccg atcagaattt caattctgcc catcaaggcc tcgtcgatta
     9181 ttacaagact ttacggcatt ttgaacaata tcatcacgat ccgatcgatg ataattttct
     9241 cgccgatttt ttagcgcgtt tcaagggcga ggatgaactc gtcagcttct accgcgaaaa
     9301 agactatgcc cgtccatatc agactgtcat cggcaaagaa tatgattttg cgccggcggc
     9361 gacgattgaa tttttgatga acgatctctt cgattttatt aataatagcg agattagctt
     9421 cgtcgtcaaa gccgtggcgg catactattt tattgactat gtcaaacctt tcgaaaatca
     9481 caatgaggaa gtcgccatca tcctcgccaa gagcatcttg gctcacaacg atatcggcga
     9541 tgtcagcgcc tttcttcctc tcgaggccct cttcgccatg cgcaatgaaa actttcgcaa
     9601 gatttttctc gaggtgcaaa aaactaacga tttgacttac gctttattgg cggttatcga
     9661 tctcgtatta aacgaggttc agaatttttt agacttgatc acgcaagtca agatgacgga
     9721 agtcaaaaaa gaatttcgct ccctcggcag cgaacttccc gccgccaaac cggcaagcga
     9781 gcccaaaccg gaaccgaaag cggtcgttaa agaagagccg gcggtggcgt cgaaaggcga
     9841 aaaagtccgc cctgccgaag ttgtcgccgt gccgcaaccc ctcgaacttc caaaggaaga
     9901 agatgtcaaa agcatcatca aaaatctcct tgaatcaaat ccgttgctac ggcccaaaca
     9961 agctgaattc tacgctcgcc atcgcaccct cggtaagtac tacacgattt cgatgtataa
    10021 aaaagagatg gatgtcgtct atgagacggc gcgaacttcg atggacaatt tggctcaact
    10081 cggttattat cgtcgtgaga atgtcaaaaa taagttcgtc tacactccga taaaaaagta
    10141 aattgaggca tcaactatgg aatcattatt cgtcgtctta gcatctgccg gatgggaaac
    10201 tccggtcatc ctcttctccc tcctgggctt ttttggactc gtcgtcctcg gcgtcatctt
    10261 tgccaagagg catatcaaag gtttacaaag taaagatgat cagcccatcg atgaggaaaa
    10321 agcgattgag gaagaattaa agcgcgttct tgaaccgatt gaagatgaaa agattcaaga
    10381 ggaaatggcg aaggccaaac atgagaaaga acccgatgcc gacaaagata agtcccaagg
    10441 ctaattccct ctacatccac atcccttttt gccatcatct ttgtcattac tgcgactttc
    10501 cgaagttgct ctatgatgag aaaatggctt tttcttatct cgaccagctg tgtaaagagc
    10561 tcgactcgta tcacatcgat gaagtcgcga cgatttatgt cggcgggggc acgccgagcg
    10621 ctttacctga tgcattattt gataagttat tggacaaaat tgcacctctt ctaaaagaag
    10681 acggagaatt cagtgttgaa ttaaatgccg aaagcacgac acaagaaaaa ctgctgatca
    10741 tgaaacatcg cggcgtgaac cgcctctcga tcggcgtcca gtcgaccgac gatcaaatcc
    10801 tcgcgagcct gaaccgaaag cacagttttg aagatgttaa aaaaatagtc aaactcgcac
    10861 gcgcagtcgg ttttaataat attaacctcg atttgatata cggtgtgcct tcgcaaagcg
    10921 aaaatatttt gaagctcgat ctcaaacgcc ttcttgcttt acatccggag catctttcaa
    10981 tctattcgct caccgttcat cccgggacga ttttcttctt aaaagggata gctgagcaag
    11041 accaagactt atcgcgcgat cattacgaca ttatccttcg cgagctgcgc aagaaaggtt
    11101 atacccgtta cgaggtgtcc aattttgcca ggccgggttt cttctcgctg cataatcaaa
    11161 cctactggca taatgaagaa tactacggcg tcggtctcgg agcctcgggc tttgtcggcg
    11221 gagtgcgcta cgacaatacg cggaatctga gcaaatatct cgccggggca tatcgtcaga
    11281 gcgaagaaca cctcacactt gaagatgacg aaaaatattt ctggatgctt aatctacgat
    11341 tagaatccgg atttgccata aaagattata ttaagagata tggagaggaa atgttaaatg
    11401 ctcgtcttta tcttttgcag gatgccatca agaacggatt gatgataaaa tcg
//
LOCUS       DAOSSL010000029        11166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5736_length_11166_cov_14.537035, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000029 DAOSSL010000000
VERSION     DAOSSL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5736_length_11166_cov_14.537035"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLH17_03605"
     CDS             <1..359
                     /locus_tag="PLH17_03605"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019721402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOR20889.1"
                     /translation="DSVLSVLAKREVQLAIMTAVELDKLAESGRMFDKDLEAILMRDE
                     GLYGVDEVIAYGVCNLYGSIALTNFGFIDKKKYGIIESLNSHGKSSGVCNTFLDDIIG
                     AIAASAASRFAHRWAR"
     gene            366..1151
                     /locus_tag="PLH17_03610"
     CDS             366..1151
                     /locus_tag="PLH17_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HOR20890.1"
                     /translation="MTLPDFASISTWIDLGLGVLVAALFTFILYRITNRKFVLFYSIL
                     SALAIVLAWFFSLELLTTILTIVLMTSATVVMFVNISRIRPLIASELKGKTMFGFRPK
                     TTEKIFDREAMYKKVNDAILALSKQKIGALLTFEKNILLTDVIKTGTLVNAPVTAELI
                     MTIFYPGTRLHDGAVVVRGDTILAASVYFTPTTKPLTGKYGSRHRAAIGISEMSDAVT
                     VVVSEETGRISLAYRGELQPVTVDTFLRVFREYMLEMPEEDEE"
     gene            1167..2498
                     /gene="glmM"
                     /locus_tag="PLH17_03615"
     CDS             1167..2498
                     /gene="glmM"
                     /locus_tag="PLH17_03615"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOR20891.1"
                     /translation="MTKYFGTDGVRGHVDEKLTVEMAYRIGRFIGQYPDGKRNRIVIS
                     RDTRISGKKLLDALIEGIRLSGGHVYDEGVSTTPSISYIVESRGFDYGIMISASHNPF
                     YDNGIKIFDAKGKKLSSDVEELIEVYMDSPTDFLPKFSGQNLGKLIEGSDLREEYISF
                     LVSKAKFDFSNLRILVDCANGSASNVAPIVFKRLGINAEFVNDKPDGLNINEHCGSTH
                     IESLIEKSKQGKYDLCFAFDGDADRLLVVKDGEVIDGDTMIYMIAISKLDRERLDNNA
                     VVITVMSNLGLKLALERHNIQYIEVDVGDKYVQAKLEELDLSLGGEQSGHIIFFHDLN
                     TGDGILTAIKMMTLYVGNGYSFDELKKDLLIFPQVLINVKVNDKKAVLSDAGLLALVD
                     EEKKALGKEGRLLVRPSGTEPLVRVMTEARTLEICNGVCHRIKDYIEKLAR"
     gene            complement(2532..2951)
                     /locus_tag="PLH17_03620"
     CDS             complement(2532..2951)
                     /locus_tag="PLH17_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20892.1"
                     /translation="MTDQQFYLTIAAIAGLGIILFALTGFINIKKGFVGIIERTGRYI
                     GTYQAGRRYFAPLVDRRVGMYKLGACRRLVEVDRYQSYFIDYEILDFKLFHYSGHDLD
                     SLVRLAKNESPDNLGLSLETRCALIGVRFIAVRENKK"
     gene            3066..3662
                     /locus_tag="PLH17_03625"
     CDS             3066..3662
                     /locus_tag="PLH17_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOR20893.1"
                     /translation="MEKKQFYDFIVKMQAISKIGLLFSKDEYAIDNYRQINDLSAKML
                     ASFTELEFERPNYFKRDIYPTPNVSTRVVVFNEKGKLLLVKEAKTGTYSIPGGWCDLY
                     DAPSQAALNEVLQEAGLEVKITRLVGVVDRTPNVAVPEYVIVFQAVPISDFRPHGYET
                     TGVGYFDLNHLPELSHKLSLEEIKRIIAACLNGDTIFD"
     gene            3742..4197
                     /locus_tag="PLH17_03630"
     CDS             3742..4197
                     /locus_tag="PLH17_03630"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOR20894.1"
                     /translation="MNRAKSYHRIFANIFDLVVIVAIALLLTFRSLISLITALINPTS
                     LDSLALYLSAFGSGALAFMAVIAYFIVLPIFWNGQTLGKRFFKIRIIKNDGSDIDFKT
                     IFLREMIRVLLFILTFGVSAFADTMVLLFSKQRIGFYDYVAATQVIDID"
     gene            4210..4932
                     /gene="deoD"
                     /locus_tag="PLH17_03635"
     CDS             4210..4932
                     /gene="deoD"
                     /locus_tag="PLH17_03635"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOR20895.1"
                     /translation="MGTPHINAEMGAFAKVVLMPGDPLRAKWIAETYLVDAKLVNEVR
                     GMLGYTGYTKNGKRVSVMASGMGQPSIGIYSHELYTGFGVESIIRIGTCGSYQSKINL
                     FDVLACQTASTDSNWSSQFDLKGGTFSAPADFDLLVRAYEVLKARKMPLHVGNILSAD
                     IFYDVDPNSWKKWANLGVMGVEMESYSLYATAARLGKRALCLLSVTDHFLKAEKATSS
                     QRQIGLGKMIEVALEVAESFAE"
     gene            4942..5562
                     /locus_tag="PLH17_03640"
     CDS             4942..5562
                     /locus_tag="PLH17_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20896.1"
                     /translation="MTDLQIAFIITASLTGLLLLFIIFYRPVKRAILRRHYVWFFARY
                     IYRIALDFDYYLINQFAYIHSNREEKTINHILFGSKWIYVITDGYYPGAISAKENDVS
                     WIYYSSRKKKAYIDNPLRINADNVNQISMITDVNKDLLISIVVINDDCHVEPFDGTSK
                     TNFLVHKSQLRHLIKALEGRDVDNIDEKALAKAVTEIHALNRNKKK"
     gene            5559..6581
                     /locus_tag="PLH17_03645"
     CDS             5559..6581
                     /locus_tag="PLH17_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20897.1"
                     /translation="MNIAQKARQTYQKYLASKLAIAFSFTIFVLASLALGILGSYLFL
                     LLVPIVILPIFICLQMTNSAFAKGMSLSQKNFFSFYRAAFTPTLSGAYQVLSSFLKAA
                     LLYFGLSFVTIFVMLQFYLTRNPFFAQQIESISALAANGNLVEALAAYENNANIIFIS
                     SIATFISGGFALLAFLHFIGRNSIVPHLALSMSSMPGRIAFSIHRQGLKFFKREFNID
                     YYRATWLGTPLILIGFAGGVLATYFLTRDPYLILLSGFAGAFIVLTPFLPYYLDVMEE
                     IFKKYKDRYITVSIDQAKKAYEEIKVAQKLSEEQQDELDKLISDLQKKADTKNQDQEE
                     KSGEEE"
     gene            complement(6614..6687)
                     /locus_tag="PLH17_03650"
     tRNA            complement(6614..6687)
                     /locus_tag="PLH17_03650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6653..6655),aa:Gly,seq:gcc)
     gene            6792..8456
                     /gene="gltX"
                     /locus_tag="PLH17_03655"
     CDS             6792..8456
                     /gene="gltX"
                     /locus_tag="PLH17_03655"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOR20898.1"
                     /translation="MDAFKQLAELIFPEVQETIEDLEKRYPPRNLKDGAQVTRFAPSP
                     TGFLHTGSLFTAMIAKKVAKDSGGIFFLRLEDTDTKREISGSGEQLISQLAEFGITLD
                     EGYLGDRDIGNYGPYRQSKRAGIYKVVIKSLIAKNLAYPCFCSTEELETLRKSQEAQK
                     VVPGYYGKYATCRYFTPQEAMAKINNGEPYVIRFRSQGDHLRKVLINDLIRGDINIAE
                     NDIDIVILKSDGLPTYHFAHVVDDHFMHTSVVTRGEEWISSLPIHVEIFKALEWTAPG
                     YAHLPLIMKMENGSKRKLSKRKDQEAAVSFFIEDGYPPRAVMAYLMSIANSNFEEWTE
                     QSNIYDIDKFTFNLDKMSLDGALFDLDKLTFFAKEVIARMSAHRLASLSKKWALNHSP
                     ELLDLINRNGEYYEQILNIEREKENPRKDFAKYSDIYSHIRFFYHDHYLELIGDMPAF
                     NSRIANNNIITFLKEFINSNDYTLDNDNWFAALKAMAAKSGFAESSKEYKANPSLFVG
                     HVGDAAEILRISLTASKNSPNLWAILQVLRCEEVNKRLQYIIEKLR"
     gene            8479..8553
                     /locus_tag="PLH17_03660"
     tRNA            8479..8553
                     /locus_tag="PLH17_03660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8512..8514,aa:Glu,seq:ctc)
     gene            complement(8587..>9629)
                     /locus_tag="PLH17_03665"
     CDS             complement(8587..>9629)
                     /locus_tag="PLH17_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOR20899.1"
                     /translation="SFEVSNTAKTSSARYRYAYDFSFFYANFTAFGYVFSGLAFDDEA
                     ELIEFDNTNTNYADKIVLVGSITSAILADLYNDGAKGAIIRDTDYNYPSIEYGQGDMF
                     DDTDFLFLYAAPESYDSLLSNISSGLNQADVSYEIDNVSKTVNNVIGILDVGASNNII
                     VSAHIDHLGRFDAEDDGYYAGALDNASGVAGMLELARIYSENAEKLAKNIIFIAYNGE
                     EAGLYGSQHYSRNLVGEKADTRAAFNLDMIGGGSNEYELEIIGNIGGLTTAVETKCAD
                     YDIDNYTIYGNQANSDHYWLGQMRIVSLSFVHFDDRFYHRPVDTEDKINYEVFANQLS
                     MIASLMLTSYNK"
     assembly_gap    9630..9729
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9730..10154)
                     /locus_tag="PLH17_03670"
     CDS             complement(<9730..10154)
                     /locus_tag="PLH17_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20900.1"
                     /translation="MYKSLAKLSTLVLALLLVACAPGGTGSDTDNDTSGTTDSESSNG
                     DDNLLTNVDGLISAENAQEHIAYLAGTEVGGRASGSLDNQLACQYVADHFEDYGLTPY
                     SEETGYFQPYLQPYTRIFSEFFSFEVSNTAKTSSARYRYA"
     gene            10266..11120
                     /locus_tag="PLH17_03675"
     CDS             10266..11120
                     /locus_tag="PLH17_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOR20901.1"
                     /translation="MDAIFLYSDAPGNGRIVHKVDYIRRRLENIFKHVQITKANSLSE
                     LDELVALACSKFDVLVFAGGDGTFHRVVNAIAKEEKRPVIGYISSGTLNDFGKNFGIS
                     RNVNKCLNIIKKGKIQKFDIGIINDKTYFAFVSAIGTFSEISYATKRKRIRAFGKMAY
                     YMNAVRDAVIPQSFTVKVHIGDNIKEHKVPFLMVLNSKYVGGFPVNLRNSLDDGKFDV
                     YLTKPGLFNGLLNYLLLKHRTAYYRVDEVTIDPNYASPWCVDGEEGPKGKITIKLLKK
                     HLKIYTNK"
BASE COUNT         3032 a         2569 c         2440 g         3025 t
ORIGIN      
        1 tcgattcggt tttaagcgtt cttgccaaac gcgaggttca gctcgccatc atgacagctg
       61 tcgaattaga taaattagct gaaagcggca ggatgtttga caaagatctc gaagcgattt
      121 taatgcgcga tgaaggcctt tatggcgtcg atgaagtcat cgcttatggc gtttgcaatt
      181 tgtacggatc aatcgctctc actaattttg gattcattga taagaagaaa tacggaatta
      241 tcgaaagcct gaatagccac ggcaaatcaa gtggcgtctg caacactttt cttgatgata
      301 tcatcggcgc tatcgccgcc agcgcggcgt cgcgctttgc tcatcgctgg gccaggtaat
      361 cgtttatgac attgcctgat tttgcatcta tttccacgtg gattgattta ggcctcggcg
      421 tcttggtcgc agctcttttt accttcattc tctatcgaat tacgaatcgt aaattcgttc
      481 ttttttattc tattttgagt gcccttgcta tcgttttggc atggtttttc tcacttgaac
      541 ttttaacgac aattttaacg attgtcttga tgacttcagc gacggttgtc atgtttgtca
      601 acatcagccg cattcgcccg ctgatcgcca gcgagctcaa gggcaagacg atgtttggct
      661 ttcgtcctaa aacgactgaa aaaatctttg atcgagaggc catgtacaag aaagtcaacg
      721 acgccatctt ggctctcagc aagcaaaaaa tcggagcttt actgacgttt gaaaaaaaca
      781 tccttttaac tgatgttatt aaaaccggca ccctcgttaa cgccccggtt accgccgagc
      841 tgattatgac tattttttac ccaggcacac gtcttcacga tggcgccgtc gtcgttcgcg
      901 gcgacacgat cttggctgcc agcgtctatt tcacgccgac gacaaaaccg ctgaccggta
      961 aatatggatc gcgccaccga gcggcgatcg gcatcagcga aatgtctgac gccgtgacgg
     1021 tcgtcgtcag cgaagaaaca ggacgcatct cgcttgccta tcgcggcgaa ctgcaaccgg
     1081 tgacagtcga taccttcttg cgcgtcttcc gcgagtatat gctcgagatg ccggaagaag
     1141 acgaagaata ataaaggaat agcaaaatga caaaatactt tggtacggac ggagtccgcg
     1201 gtcatgttga cgaaaaactc actgttgaga tggcttatcg aatcggacgc ttcatcggtc
     1261 aatatcccga tggcaaacgc aatcgcatcg tcatctctcg cgatacgaga atatccggta
     1321 aaaaattgct ggatgcctta atcgaaggca ttcgcctcag cggcggacac gtttatgacg
     1381 aaggtgtctc gacgacgcca tcgatctcct acatcgtcga atcaagaggt tttgattacg
     1441 gcattatgat cagcgcctca cataatccgt tttatgataa tggcatcaaa atatttgatg
     1501 cgaaaggaaa aaagctctcg tccgacgtcg aggaactgat cgaagtctac atggattcgc
     1561 cgaccgattt ccttcctaaa ttcagcggcc aaaatctcgg caagctcatc gagggcagcg
     1621 atcttcgcga agaatacatc tcctttctcg tctccaaagc caaatttgat ttttcaaatc
     1681 tccgcatcct cgtcgattgc gcgaacggtt cggcttcaaa tgtcgcgccg atcgttttca
     1741 agcggctggg tattaacgcc gaatttgtta atgataagcc tgacggatta aacattaatg
     1801 agcactgcgg ctcgacgcac atcgaaagcc tgattgaaaa gagcaaacag ggtaagtacg
     1861 atttatgctt tgccttcgat ggcgatgccg accgccttct cgtcgttaaa gatggcgaag
     1921 tcatcgatgg cgacacgatg atctatatga tcgccatctc caaactcgat cgcgaaagac
     1981 tcgacaataa tgcggtggtc atcaccgtga tgtcgaatct cggtctcaaa ttagccttag
     2041 aacgccacaa cattcagtac attgaagttg atgtcggcga taaatatgtg caggccaagc
     2101 ttgaggaatt ggacttatcc cttggcggcg agcaatctgg acacatcatt ttcttccatg
     2161 acttaaatac tggcgacggc attttaaccg ccattaaaat gatgacctta tatgttggaa
     2221 acggctattc attcgacgaa ctcaaaaaag atttactaat ctttccgcaa gtgctcatca
     2281 atgttaaggt caacgataaa aaagcagtgt taagcgatgc gggtttactc gctcttgtcg
     2341 acgaagaaaa aaaggcgctc ggcaaagaag gccgactgct cgtccgcccg agcggaaccg
     2401 aaccactcgt tcgcgtcatg accgaagccc ggaccctcga aatttgtaac ggcgtctgcc
     2461 acagaataaa agattatata gagaagttag cgcgttaatt atttataatt aaaaatagag
     2521 atgaacattc tctatttttt attctcgcga acagcgataa agcgcacgcc gatcagggca
     2581 caacgcgttt cgagcgataa tccaagattg tctggtgatt catttttggc cagtctcacc
     2641 aagctgtcga gatcgtggcc cgagtagtga aataatttga aatcgagaat ttcataatcg
     2701 ataaaatacg attgatagcg atcgacttcg acgaggcggc ggcacgcccc gagtttatac
     2761 attccgacgc ggcggtcaac taaaggcgcg aagtagcggc gtccggcttg gtaggtgccg
     2821 atgtagcgac cggttcgttc gatgataccg acaaagccct tcttaatatt aataaaaccc
     2881 gtcaaggcaa aaaggatgat gccgagtccg gcaatcgcgg cgatcgtgag gtaaaattgc
     2941 tgatcagtca tcttgcaaat agtttatcat gaatttacct gcatataatc aaaagatgat
     3001 tgccgaccgc cgcgacttaa caattgttaa tcgctccgtt tggcgctata ttataaagag
     3061 aattaatgga aaagaaacaa ttttacgatt tcatagtgaa aatgcaggca atctctaaga
     3121 ttggcctttt attttccaaa gacgaatacg ccatcgataa ctatcggcaa atcaatgatt
     3181 taagcgcgaa gatgctcgcg tcgtttacgg agcttgaatt tgaacgcccc aactacttta
     3241 agcgcgatat ctacccgact ccgaacgtct cgacgcgagt tgttgtcttt aacgaaaaag
     3301 gcaaactcct tcttgttaaa gaagcgaaga cgggaacata ttcaattccc ggcggatggt
     3361 gcgaccttta tgatgccccc agtcaggcag cgctcaatga agttcttcaa gaggccggac
     3421 tcgaagttaa aatcacccgc ctcgtcggcg tcgttgaccg cacgcccaat gtcgcggttc
     3481 ccgaatatgt catcgttttt caagcggtgc cgatatctga ttttcgcccc cacggctatg
     3541 agacgaccgg tgtcggatac ttcgacttaa atcacctgcc cgaactctcg cataaattat
     3601 cgcttgaaga aattaagcga atcatcgcag cctgcctcaa cggagacacg atttttgact
     3661 aaccgctttt taagaatttt tcttaaatgt gtataaagag tagccgtttt ttgttaaagt
     3721 taataagaag gaccagcaag aatgaaccgc gctaaatctt accatcgcat cttcgccaat
     3781 atctttgatt tggtcgtcat cgtggcgatc gctttattgc tgacatttcg ctcgctcatc
     3841 tcgcttatca ccgcgctgat taatccgacg agcctcgaca gtctggctct gtatctgtca
     3901 gcttttggtt cgggagccct ggcctttatg gcggtcatcg cctattttat cgtgctgccc
     3961 atcttttgga acgggcagac gctcggcaaa cgcttcttta aaatacgcat cattaaaaac
     4021 gatggctctg acatcgattt caagacgatt tttttgcggg aaatgattcg tgttttactc
     4081 tttattctga cgtttggcgt cagcgctttc gccgacacga tggtcttgct tttttctaaa
     4141 cagcgcatcg gtttttacga ctatgtcgcc gctacacaag taatcgatat cgattaagag
     4201 gaggaattta tgggaacacc acacatcaat gccgaaatgg gcgcctttgc taaagtcgtc
     4261 ttaatgccgg gcgatccgct ccgcgccaaa tggattgccg agacatattt ggtcgacgcc
     4321 aagttagtta acgaagttcg cggtatgcta ggctataccg gctataccaa gaacggaaag
     4381 agagtctcgg tgatggcttc ggggatgggt cagccttcga tcggcatcta ctcgcacgaa
     4441 ctttacacgg gtttcggtgt cgaatccatt attcgcatcg gcacctgcgg atcttaccaa
     4501 agcaaaatca acctattcga tgtcctcgct tgccagacgg cttcgaccga ctcgaactgg
     4561 tcctcgcaat tcgacttaaa aggcgggact ttttccgcgc cggccgattt tgatttatta
     4621 gtgagagctt atgaagtgct aaaagcgaga aaaatgccgc ttcatgtcgg caatattctc
     4681 tcggccgata tcttttatga tgtcgatcca aactcctgga aaaaatgggc gaatctcgga
     4741 gtgatgggcg tggagatgga atcgtacagc ttgtatgcga cagccgcccg ccttggcaag
     4801 cgcgccttat gtcttctttc cgtcaccgac catttcttaa aggccgaaaa agcgacgagc
     4861 agccaacggc agatcggcct cggcaaaatg attgaagtcg cccttgaagt cgccgaatcg
     4921 ttcgctgaat aaaacttgtt aatgaccgat ttacaaatag cgtttatcat cacggcttcg
     4981 ctcaccggat tactgctcct ttttatcatc ttttaccgac cggttaaaag agccattttg
     5041 cgccgccact atgtttggtt ttttgctcgc tatatctatc gtatcgctct tgattttgat
     5101 tattatttaa tcaatcaatt tgcctatata cactccaacc gcgaagagaa gacgattaac
     5161 cacatcctgt ttggaagcaa atggatctat gtgattacgg acggctatta tcccggcgcc
     5221 atcagcgcca aagaaaacga tgtctcgtgg atttactact caagccgcaa gaaaaaggct
     5281 tatattgata acccgcttcg catcaatgcc gacaacgtca accagatctc gatgattacc
     5341 gatgttaata aagatttgtt aatatctatt gttgtcatca atgacgattg ccatgtggaa
     5401 ccgtttgatg gcacttcaaa gacaaacttt ctcgtccata aaagccagct tcgccatctg
     5461 attaaagccc ttgaagggcg cgatgtagac aatatcgatg aaaaagctct ggctaaagca
     5521 gtgacagaaa ttcacgccct aaaccgcaat aagaaaaaat gaacatcgca caaaaagctc
     5581 ggcaaaccta tcaaaaatac ttggccagca aattagccat cgccttttcc tttacgattt
     5641 ttgttttggc tagtctagcc ctcggcattc tcggaagcta cttgtttctc cttctcgtcc
     5701 caattgtcat tttaccgata tttatatgtc ttcagatgac caatagcgcc tttgccaaag
     5761 gaatgtcgct tagccagaaa aactttttca gtttttaccg cgcggctttc acgccgacgc
     5821 tcagcggagc ttatcaggtt ctatcctcat ttttgaaagc ggcgcttctc tattttgggc
     5881 tttcctttgt taccattttt gtcatgctgc aattctattt aactcgaaat ccgttttttg
     5941 cccagcaaat cgagagcatt tctgccctcg ccgctaacgg caatctggtc gaagccctcg
     6001 ccgcctatga aaataatgcc aacatcatct tcatctcgag catcgccact tttatcagtg
     6061 gtggttttgc tctgctggcc tttctccact ttatcgggcg caattccatc gtccctcatc
     6121 tcgccttgtc gatgtcttcg atgccggggc gaatcgcctt ttcgattcac cgtcagggat
     6181 tgaaattttt caagcgagaa ttcaatatcg attattatcg cgccacgtgg ctgggaacac
     6241 ccttgattct catcggcttt gccggcgggg ttttagccac ctattttttg actcgcgacc
     6301 catatttaat ccttctctcc ggctttgccg gcgcctttat tgttctcacg ccgtttcttc
     6361 cttattatct cgatgtgatg gaagaaatat ttaagaagta taaagaccgc tatatcaccg
     6421 tctcgatcga tcaagctaaa aaagcatacg aagagatcaa agttgcgcag aagctgagcg
     6481 aagagcagca agatgaactc gataagctga taagcgatct gcagaaaaag gctgacacca
     6541 aaaatcagga ccaagaagaa aaaagcggtg aagaggaata aaaaaagccg actgagcggc
     6601 tatcttgagc atctggagca ggtgacggga atcgaacccg cgtaactacc ttggcaaggt
     6661 agtgttctac cattgaacta cacctgcatg aaagcgatgc gagtatcatt ataaagaact
     6721 gtacgcgatt attcaagcac caaaaaatgt gataaaatat attattactc ttagagtaaa
     6781 ggaaaaacgc tatggatgcc ttcaaacaac tagccgaact gatctttccc gaagtccaag
     6841 aaacgatcga ggacctagaa aaacgctacc ctcctcgcaa tctaaaagat ggcgctcagg
     6901 tgacgcggtt tgcgccctcc ccgaccggct ttttgcatac cggctccctc tttacggcga
     6961 tgatcgccaa aaaagtcgcc aaggatagcg gcggcatctt ctttttgcgg ctcgaagaca
     7021 ccgataccaa gcgcgagatt tcaggaagcg gcgaacagct gatttcgcaa cttgcggaat
     7081 ttggtatcac ccttgacgaa ggctatctcg gcgaccgcga tatcggaaat tatggcccct
     7141 atcggcagag taagcgcgcc ggaatctata aagttgtcat caaatccctg attgccaaga
     7201 atctggctta tccttgcttc tgctccaccg aagaactaga aactctccgc aagagtcaag
     7261 aagcgcaaaa agtcgtcccc ggatattacg gcaaatacgc gacatgccgt tattttactc
     7321 ctcaggaggc gatggccaag attaataacg gcgaacctta cgtcattcgt tttcgcagcc
     7381 aaggcgacca ccttcgcaaa gtgctgatta atgatcttat tcgcggcgat ataaacattg
     7441 ccgagaatga tatcgatatc gtcattttaa aatccgacgg tcttccgact taccattttg
     7501 cccatgtcgt cgatgaccat tttatgcaca ctagtgtcgt cacgcgcggc gaggaatgga
     7561 tttcctctct gcccatccat gtcgagatat ttaaggctct tgagtggacc gctcccggct
     7621 atgcccatct tccgttaatt atgaagatgg aaaacggcag caagcgcaaa ttatcgaagc
     7681 gcaaggatca ggaagcagcg gtcagtttct tcatcgaaga cggctatccg ccccgggccg
     7741 tgatggccta tttgatgtcg attgccaact cgaattttga ggagtggacc gagcagagca
     7801 atatctatga tattgataag ttcaccttta accttgataa gatgtcgctg gacggcgccc
     7861 ttttcgatct tgataaacta acttttttcg ccaaggaagt aatcgcccga atgagcgcgc
     7921 atcgcctggc ttcactttca aaaaagtggg ctcttaatca ctcgccagaa ctgcttgatt
     7981 taatcaatcg caacggtgag tattatgaac agattctcaa tatcgaacgc gaaaaagaaa
     8041 atccgcgtaa ggattttgct aagtatagtg atatctactc tcatatccgc ttcttttatc
     8101 acgaccacta tctcgaatta attggcgata tgccggcctt taacagtcgg attgctaata
     8161 ataatatcat caccttttta aaagaattca ttaactcgaa cgattacact cttgataacg
     8221 acaattggtt tgctgctctt aaggcaatgg cggcgaaaag cggctttgcc gaaagcagca
     8281 aagaatataa ggccaatccc agcctctttg tcggccacgt cggtgatgct gccgaaattc
     8341 ttcggatttc tttaaccgcc agcaaaaatt cgccaaacct ttgggctatc ctccaggttc
     8401 ttagatgcga agaagttaat aaacgcctgc aatatattat tgaaaagttg cgttaaaact
     8461 gcttaactaa tactcgtcgg tcctatggtc aagcggctaa gacgcaaccc tctcaaggtt
     8521 gaatcgcggg ttcgattccc gctaggatca ccaagtggaa aattatctat cgaaagatag
     8581 atttttttat ttgttataag aagtaagcat caagctagct atcatagata gctgattggc
     8641 aaaaacttca taattgatct tatcttcggt atcgacagga cgatgataaa aacggtcgtc
     8701 aaaatggacg aacgaaagcg agacgatgcg catctgccca agccagtagt gatcgctgtt
     8761 ggcttggttg ccatagatag tgtagttatc gatatcgtaa tcagcgcatt tagtttcgac
     8821 agccgtggta agtccaccga tatttccgat tatttctagt tcgtactcat ttgaaccgcc
     8881 accgatcata tcgaggttaa aggcggctct cgtatcggct ttttcgccaa cgaggtttcg
     8941 cgaataatgt tgtgaaccat agaggccggc ctcttcgccg ttataggcga taaaaataat
     9001 gtttttagcc agtttttctg cgttttcgct atagatgcgg gcgagctcga gcattccagc
     9061 cacgcccgaa gcattatcga gagccccggc ataatagcca tcatcttcag cgtcgaagcg
     9121 gccgaggtga tcaatgtggg cggagacaat aatgttattg gaagcgccga catctaaaat
     9181 tccgatcacg ttattgactg ttttactgac gttatcaatt tcataagaga catcggcttg
     9241 attaaggccg cttgaaatat ttgatagcag tgaatcatat gattccggcg cggcatacaa
     9301 aaagaggaaa tcagtgtcat caaacatatc cccctgacca tattcaatgg acggatagtt
     9361 ataatctgtg tcgcgaataa ttgctccttt ggccccatca ttataaaggt cggctagaat
     9421 cgccgaagtt atagatccga ccaagacaat cttgtcagca taatttgtgt ttgtattatc
     9481 gaattcaatc aactcagctt catcatcaaa agcaagtccg gagaagacat agccaaatgc
     9541 tgtaaaattg gcatagaaga aactaaaatc atacgcatat cgatagcggg ccgatgaagt
     9601 cttggccgtg ttcgaaactt caaaagagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnng catatcgata gcgggccgat gaagtcttgg ccgtgttcga aacttcaaaa
     9781 gagaaaaatt cgctaaatat tcttgtataa ggttgaaggt atggctggaa ataaccagtt
     9841 tcttctgaat acggggtaag accataatct tcgaaatggt cggcgacata ttggcaggca
     9901 agctgattgt cgagacttcc cgaagctctt cctccgactt cggtgccggc taaatatgcg
     9961 atgtgttcct gagcattttc agccgatatc agaccatcga cattcgtgag gagattatca
    10021 tctccgtttg atgattcaga atcggttgtt cctgaagtgt cgttatcagt atcgcttcct
    10081 gttcctcccg gggcacatgc cacgagtaat aaagccaaaa cgagagttga tagttttgct
    10141 agtgatttat acatttttta gcctctatga attagtaatg taaatactat tcttatatag
    10201 aatacttttc aagtttgttt agtgcatttt agtttaaaag ccctttcatg tggtaagata
    10261 aatgcatgga tgccatcttt ttgtattctg atgcccctgg aaacggtcgc atagtccata
    10321 aagttgatta tattcgccgc cgtctcgaga atatttttaa acatgttcaa attactaagg
    10381 caaatagtct tagcgaacta gatgaattgg ttgctcttgc ctgttcaaag tttgacgtcc
    10441 tggtcttcgc gggcggcgac ggcacttttc atcgcgttgt gaatgcgatt gccaaagaag
    10501 aaaaaaggcc ggtcatcggc tatatatcca gcggtactct caatgatttc ggtaagaatt
    10561 ttggcatcag ccgcaacgta aacaagtgtc ttaacatcat caaaaaaggc aagattcaga
    10621 agtttgatat cggcattata aacgataaga catattttgc atttgtgtcg gccattggca
    10681 cttttagcga aatctcttac gcaacgaaga gaaaaagaat tcgcgccttt ggcaaaatgg
    10741 cctactacat gaatgccgtc cgagatgctg tcatcccgca gagttttaca gtaaaagttc
    10801 atatcggtga taatatcaaa gagcataaag tacctttcct aatggttctt aacagtaagt
    10861 atgtcggcgg atttcccgtc aatctaagaa actcgcttga cgatgggaaa tttgatgtat
    10921 atcttacaaa gccagggtta tttaatggcc ttcttaatta cttattgtta aaacacagaa
    10981 ccgcttatta tcgcgtcgat gaagtcacca tcgatccaaa ttatgcctcg ccatggtgtg
    11041 ttgatggcga agagggccca aaaggaaaaa tcacgataaa gttgctcaaa aaacatctta
    11101 agatctatac aaataagtga ttttattgtt gaaaaaagtg cgttatttgc gcattttttt
    11161 taaaat
//
LOCUS       DAOSSL010000030        10998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5844_length_10998_cov_16.348807, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000030 DAOSSL010000000
VERSION     DAOSSL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5844_length_10998_cov_16.348807"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..231
                     /locus_tag="PLH17_03680"
     CDS             34..231
                     /locus_tag="PLH17_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOR20902.1"
                     /translation="MLGKVKSFNKEKGYGFITGEDGKDVFFHYSALQMGSFKTIEVGA
                     SVEFEAQDSERGLRASKVVKL"
     gene            complement(321..1169)
                     /locus_tag="PLH17_03685"
     CDS             complement(321..1169)
                     /locus_tag="PLH17_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOR20903.1"
                     /translation="MHHKKIDIMKMASYIILTLGLIFVLLPMVYMLVSAFKDNELTFT
                     YPPELFPSIGELTLDNFRYIFDNTKFWTYLGNTILVASMTVLISIVISTTLGYVFARL
                     YIPGKKYIFGFLLSIMMIPGLALIVPQFELAVRFNLYNSIWGVILFYAAWVTPFSTFL
                     IKGYIEDNVPRELEDSIKVDGGSVFVIYRHIILPLTSPVVASISILNFLFPFEELGWA
                     TAILKSDKYRTLPVAITMFFQSHARTDWGYVFAMTTLAMIPVLLIYVLLQRYFVSGLS
                     SGAIKE"
     gene            complement(1171..2067)
                     /locus_tag="PLH17_03690"
     CDS             complement(1171..2067)
                     /locus_tag="PLH17_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOR20904.1"
                     /translation="MKAFLVRFKKKFDWRVLVLFLPSFIYVCMFTFYPMIKMFLMSFT
                     DWHIGYNQESPIVWFDNYVRVFQDPVAITAIVNTLFYLVITVPLQMAIGLFIALLINK
                     ITHLSVTFRVFYYLPVVTSWVVVALLFRYIFMNDGLLNYFLVDVLHFTGEKVGWLSTR
                     FMALTATGVLGIWKGIGWNMVIFLAALQAVPKEYYEAASIDGANAFQKFIYITIPSIR
                     GTIAFALVMLTIGAFNTYTPIAVLTEGNPYHQTEVVLTWMNFKTFSTLDMGYSAALSI
                     ILMMMIIITTMVMMKFIYRKGR"
     gene            complement(2155..3384)
                     /locus_tag="PLH17_03695"
     CDS             complement(2155..3384)
                     /locus_tag="PLH17_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOR20905.1"
                     /translation="MKKISALLLTAMTLALTTGCTDSDIVTIDFWHNYSAGDGQVEVL
                     EDLIAEFEEDHPNIHVEHVFLEWSALRQGVILGADTGLLPDLLRGDIAFVPQFQSLNV
                     LEELSAYDDYETVANTVLAQPNSSNLMNGKYYGLASNTNTKILFYNEDILEAADVDVP
                     TTLDEMWDAASAVSSETVIGYVEPWTGIWNVGPYIWSEGGDILSPDNTTATGYLNGAI
                     PVAVISKMRQMYADGHLTGSSMNPSAMGDTDGWAAGKYAMELDGPWRQASNNAAGINY
                     GAIPLPPGDEGSISVLGGENFMMFKTSPDEEKAAAWEFTKFLAGEHAQVEMAKHGQMP
                     VNKDALENAEAVTAMPLLPVFAEALETARARPVIPEWGDIENIIAASVAEAITGAKAV
                     QTALDEAVTAINAILAG"
     gene            complement(3466..5157)
                     /locus_tag="PLH17_03700"
     CDS             complement(3466..5157)
                     /locus_tag="PLH17_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HOR20906.1"
                     /translation="MKPLAPLHKETKEYIYPVSRREVCIKVEFAFPGNYDIRLVYWKR
                     FRKITKKSKRMLAVAKFGTSKYYTVNLGFSDVIRYLNYIFEIKVGHRSYYYSPEGLTF
                     SYPLKNFEFQNINESDVFIVPNSYAGQLAYHIIPERYHNGNKDNDPIDVVAWDATPTR
                     TNYFGGDLQGIAMKIPYLKELGVKTIFLTPIFKASSNHKYDTVDYYAIDPVFGTEEEF
                     RHLVSALHNEGMRLILDGVFNHIGYYSRQFQDVISKGKKSPYWRWFFVYGDKVDTEKI
                     NYECVGDYMWMPKLNTANEDVQTFFEEVGRYWIERFQIDGWRLDVGDELDFYFRKHFR
                     RVIKKHHPEVLIMAETWHNGLDLLRGDEVDSIMNYRLRDAIIDYLVKKSISLNTFIER
                     IETICFDYPKQVHHILYNLIDSHDTPRFLTVAGNDKSLLFLAIVMQMLLPGMPVIYYG
                     DEIGMAGETDPLCRAGMAWDYQDSEVYDFYKKMIEIRSSCGELATGDFAIIHNHQDVF
                     AFTRSNNEHVTLVIINPQDKAKTFALDALKAIELGFTGEIRLDVAPKSYVIRRNI"
     gene            complement(5159..6181)
                     /locus_tag="PLH17_03705"
     CDS             complement(5159..6181)
                     /locus_tag="PLH17_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR20907.1"
                     /translation="MATIKDIAKNLDLSFSTVSRALNDHPAISKKTKQLVEAEARRLN
                     FAFNTSAKSLTSNKAYTITLLVDIDDEQSFQNPYFYEEMHGIERYVYQKEYSLIVANI
                     KTAPKKQNMIDFLVKSKRTDGIILPSNIIDDELVAKLCEHNIPFVSIGQPSVSSSPIS
                     WVDIDNFRGGQSAAQHLLQSGCHKIAFLGHNPKKFFSQKRYEGYLSALATHNIQLNKE
                     LITTCGNSKEDGYECARELFKKHLDIDGVICGDEIISIGVIRALSELGKKIPEQVSLI
                     SFDNGQIAQLSFPSITTIDVDVFELGYQSAKLLFDQIEHTSLKNQGILIGTAIELRET
                     TRKKES"
     gene            complement(6416..7279)
                     /locus_tag="PLH17_03710"
     CDS             complement(6416..7279)
                     /locus_tag="PLH17_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOR20908.1"
                     /translation="MNNCIVRINESLDSFSKQVGKVARYVLDHQEEVIQMTISELAKK
                     SKSSTATIIRFANLLGFKGYRDFIKSLYHDVVSNTLSEDNIYDIDNKTPKESTIRQTI
                     DMVSKLNIEALMSTLKIVDEKCVERAVNAINTANRVAIYALSGSLIAAQDALFKFDRL
                     GINCRLYDTPHSQILSAATLNAEDAAIFLSYSGETKDIIAAAEIAANRRIKCISITKY
                     GENTLTKTCNINIQHSSLGKGFRSFSTRSRVVQENIIDILFVALAQRRGDHLKKYYQL
                     FDYAAPNGIKK"
     gene            complement(7444..8190)
                     /locus_tag="PLH17_03715"
     CDS             complement(7444..8190)
                     /locus_tag="PLH17_03715"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOR20909.1"
                     /translation="MFLNFKTIDNIRDLGGIKTADGHTIVLGRLLRSSDLHKLSAKEL
                     DILKNKYNLRVVIDFRSTNSSIHRRDLIDDTIKYYHKYTLKFLETNSYNQEITIDPDE
                     FFMGVYRSLALQEEAMEAYRKFFRIVIENDEGAILWHCTSGKDRTGIAAALLLRILGC
                     DMETIYQQHFRTNEITLPILDEKLKTVDPSDKKQIKYYEAYYIAKKDYIDEYFKAVNS
                     KYGSLDEYIQNQLKVSEFDQQVLRTRYLIN"
     gene            complement(8207..9139)
                     /locus_tag="PLH17_03720"
     CDS             complement(8207..9139)
                     /locus_tag="PLH17_03720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOR20910.1"
                     /translation="MNNKNETVEIVETPKKNLPKPYLLTRIAASFLDFLLACLLFAGI
                     EAALYFTIFEPLGYHQLMDESQAVLQDSHLYVLDDDKGLLTITEAYDENLSPEENYDV
                     PLTYYYTNDVRAIADNRLARYHNSKLVSGYFVEESEGVFVRADGADDAEVKTFFAGAY
                     DDAVTFLETDPVYLNGVEKTFYIVIFTSLFSATLGMAIIFLLIPLLMKNGQTPFKLVF
                     KLCLADARDDTRVKKGQVAIRFVILLLFNIWVPILLFARFSYFTLIPIFISIVLMSLT
                     KTFSGPHDYVARTYIVSNRDIVIPERSTPKELNQ"
     gene            complement(9202..10317)
                     /locus_tag="PLH17_03725"
     CDS             complement(9202..10317)
                     /locus_tag="PLH17_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR20911.1"
                     /translation="MPRIELINVTKKWGKFVAVDNLSMIIEDREFVTLLGPSGCGKTT
                     TLRMIAGLETPTSGKIIIGDRVVFDSDKGINVSPSNRDIGFLFQNYALWPHMTVYKNI
                     SFGLENLKWKKEQINARVEEVMKTLQISEFRDRYPSELSGGQQQRVAIARTLAPNPQV
                     LFMDEPLSNLDAKLRGEMRTELKRLHRDTDSTFVYVTHDQLEAMTLASRTCLINKGLL
                     QQYDKPLRVYNHPTNLFVADFVGSPTMNFLKAKAKKVSDTVINLEVLGLNIKYTSNEP
                     VKLTSENVIIGIRPEFLLIKDNGAIEGLAYSTLPSGMETTVKINLKDTILSAVQFGAV
                     DYAFDQKISFDIIGKSIVLFDEESKVSIALGACEVLK"
     gene            complement(10323..>10998)
                     /locus_tag="PLH17_03730"
     CDS             complement(10323..>10998)
                     /locus_tag="PLH17_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR20912.1"
                     /translation="IFHNYTIWRAFGRSVLVAAVVALVAGTFGILIGYAVSKNRRNRI
                     ANYVSSTAFLPYLIPALSFGAIFFSLTFKQGFQWLNVASTNNEWSAVAICILVASIKF
                     LPFASRSGTNAMLQVSGEIEEAAIITGCPWWKRMTHVLFPIQKSSLISGYLLPFISCM
                     RELTLFVLITSSFSLITNVLQFYNVYGMTQMSNAVNLLIVVFVIGVNFLINKLTGASI
                     DKGIGG"
BASE COUNT         3031 a         2366 c         2604 g         2997 t
ORIGIN      
        1 ttacttcgtt atgttattca gaaaggtgtt atcatgctgg gaaaagttaa aagcttcaac
       61 aaggaaaaag gttatggttt cataaccggt gaagacggaa aagacgtctt cttccattac
      121 tccgcacttc aaatgggctc attcaagacc atcgaagtcg gcgcaagtgt tgaattcgaa
      181 gcgcaagatt ccgaacgcgg actcagagct tccaaagttg ttaagctata atcgttaaaa
      241 ctttatagaa acaaactaag ccgcttttag cggcttttta tttaccaatt attcattaac
      301 acaattattc attaacgtta ttattcttta atcgctccgc ttgagaggcc gcttacaaag
      361 tagcgctgga gcaatacata tattagcagc acgggaatca tcgccagggt cgtcatcgca
      421 aagacatagc cccagtccgt tctggcgtgc gactggaaaa acatcgtgat cgcaaccgga
      481 agcgtacgat atttatcgga cttaagaatc gccgtcgccc agccgagttc ttcaaaaggg
      541 aacaggaaat tcaaaatcga gatactggcg acgactggag aagtcagcgg caagatgatg
      601 tgccggtata tgacaaacac cgagccgcca tcgactttaa tcgaatcttc gagctctcgc
      661 ggcacattat cttcgatgta gcctttgatt aagaaggtgg aaaagggcgt gacccatgcc
      721 gcataaaaca agatgacacc ccaaatgctg ttataaaggt taaagcggac cgccaattca
      781 aactgcggaa caatcaacgc taaaccggga atcatcataa tcgaaagcaa gaaaccgaag
      841 atgtatttct tgcccgggat gtaaaggcgg gcaaaaacat aaccgagtgt cgtcgaaatg
      901 acaattgaaa tcaaaacggt catcgaggcg accaagatgg tattgccgag ataagtccaa
      961 aatttcgtgt tgtcgaatat gtagcgaaaa ttatccagcg tcagctcgcc gattgacggg
     1021 aacagctccg ggggataagt aaaagttaac tcgttatctt taaatgccga aacgagcata
     1081 tataccatcg gaagcaagac aaaaatcaaa cccagagtca ggatgatata actcgccatt
     1141 ttcatgatat cgattttctt atggtgcatc tcatcttccc tttcgataga taaatttcat
     1201 catgaccatc gttgtgatga taatcatcat cattaaaatg atggataaag ccgcggaata
     1261 acccatatca agagtcgaaa aggttttgaa attcatccat gtcaaaacaa cctcggtttg
     1321 atgatacgga tttccttcgg tcagcacggc aattggagtg taagtgttaa acgccccgat
     1381 ggtcagcatc actaaagcaa aggcaatcgt cccgcgaatc gagggaatgg tgatgtagat
     1441 aaacttctga aaagcgttag ccccatcaat cgaggcggct tcgtaatatt ctttaggaac
     1501 agcttgaagg gcggccagaa aaatgaccat gttccagccg atgcctttcc agatgccgag
     1561 gacgccggtc gccgtcaaag ccatgaaccg cgtcgatagc cagccgactt tctcgccggt
     1621 gaagtgcagg acatcgacaa gaaagtaatt gagcaaaccg tcattcatga aaatatagcg
     1681 aaaaaggagg gccaccacca cccacgaggt aacgaccgga aggtaataga agacgcggaa
     1741 ggtgactgac aaatgggtta ttttattaat tagcaatgcg ataaaaaggc cgatggccat
     1801 ttgcagcgga acagtgatta ctaagtaaaa aagggtattt acgatggcgg taatcgccac
     1861 cggatcttga aagacgcgaa cataattatc gaaccacaca atcggcgatt cctgattata
     1921 gccgatgtgc caatcggtga aactcattaa aaacattttt atcattggat aaaaagtgaa
     1981 catacacaca taaataaaac taggcagaaa gagaactaaa acccgccagt cgaatttctt
     2041 cttaaaacgg acgagaaacg ctttcatcgt gctgcctact cctattttta ttatttggga
     2101 agagtcacaa agttcaaaca aacgttgtgg ctccccccgt tatttgattg attgctaacc
     2161 ggctaaaatg gcgttgatgg cggtcaccgc ttcatctaaa gcggtttgaa cggccttagc
     2221 tccggtaatc gcttcggcca ccgacgcggc aatgatattt tcaatgtcgc cccattccgg
     2281 tataaccggg cgggcacggg cggtttctaa ggcttcggca aagacgggaa gaagaggcat
     2341 ggccgtgacg gcttcggcgt tttccaaagc gtctttgtta acaggcattt gcccatgttt
     2401 ggccatctcg acttgcgcgt gttcgccggc taagaatttg gtgaattccc aggccgcggc
     2461 tttttcttcg tctggtgaag ttttgaacat catgaagttc tcgcctccga gaaccgagat
     2521 ggatccttca tcccccgggg gaagaggaat ggccccgtag ttaataccgg cggcgttatt
     2581 tgaggcttga cgccacggtc catcgagttc catcgcatac tttccggccg cccagccatc
     2641 ggtgtcgccc atcgcggacg ggttcatcga tgatccggtt aaatggccat cggcatacat
     2701 ttgtctcatc ttgctgatga cggcgacggg aatcgccccg ttgaggtatc cggtcgcggt
     2761 cgtgttgtcg ggtgataaga tgtcgccacc ttcactccaa atgtagggtc cgacattcca
     2821 gatgccagtc cagggttcaa catagccaat tacggtttcg gatgagacgg cgctggcggc
     2881 atcccacatc tcgtcgagag tggttgggac gtcaacgtcg gcagcctcta aaatatcttc
     2941 attatagaag aggattttcg tattcgtatt tgatgctaaa ccataatatt tgccgttcat
     3001 gagattagat gagttgggct gcgccaaaac cgtattcgcg accgtctcat aatcatcgta
     3061 agcggacaat tcttcgagga cattcaggct ttggaattgc ggtacaaagg cgatatcccc
     3121 gcgtagtaaa tcaggcaata agcctgtatc ggcgcctaag atgacgccct gacgaagtgc
     3181 gctccattcc aggaagacgt gctcgacgtg aatattcgga tggtcttcct caaattcagc
     3241 aatgagatcc tcaaggactt ctacttggcc atcgccggcg gaataattgt gccaaaaatc
     3301 gatggtgaca atatcgctat ccgtacagcc ggtggtcaac gcgagggtca tagctgtcag
     3361 taatagagca gagatttttt tcataatcgt ctcctttctt tatattaata tcgccgatac
     3421 ctctgggggc ccgtccaacg cggcatctaa cgaattaata acttattaaa tgttccttct
     3481 gatcacatac gacttcgggg caacatctag tcgtatctcg cccgtaaatc cgagttcgat
     3541 ggccttgagg gcatcaaggg caaaggtttt tgctttatcc tggggattga tgatgacaag
     3601 ggtgacgtgc tcattgttgc tgcgagtgaa agcaaagaca tcctgatggt tgtgaataat
     3661 ggcaaaatcg ccagtcgcca gttctccgca ggagctgcga atctcaatca tttttttata
     3721 gaaatcatat acttccgaat cttggtaatc ccatgccatg ccggcgcggc agagaggatc
     3781 ggtttcaccc gccataccga tttcatcgcc gtaataaatg accggcatac ccggcaatag
     3841 catttgcata acaatcgcca aaaagaggag tgatttatcg tttccggcga ccgtcagaaa
     3901 gcgcggggtg tcgtggctgt cgatcaagtt atataagata tgatgcactt gtttggggta
     3961 gtcaaaacaa attgtctcaa tccgttcgat aaaagtattg agtgaaatac tttttttgac
     4021 gaggtagtca ataattgcat cgcgaaggcg gtaattcata atcgaatcga cttcgtcgcc
     4081 gcgcagcaaa tcgagtccgt tatgccatgt ctcggccatg attaaaacct caggatgatg
     4141 ctttttgatg acgcggcgga aatgcttgcg aaaatagaag tcgagttcat cgccgacatc
     4201 aagccgccag ccatcaatct ggaagcgttc aatccaatag cgaccgactt cttcaaaaaa
     4261 tgtctgaaca tcctcatttg ccgtattgag ttttggcatc cacatatagt cgccgacgca
     4321 ctcgtagttg attttttcgg tatcgacttt atcgccatag acgaagaacc accgccaata
     4381 cggcgatttt ttgcctttgc ttatcacatc ctgaaactgc cgggaataat agccaatatg
     4441 attaaagacc ccgtcgagta tcagacgcat tccttcgttg tgcaaagcgc tgacgagatg
     4501 tctgaattct tcttcggtgc caaacaccgg atcgatggca tagtaatcaa ccgtgtcgta
     4561 tttgtgattt gacgaagcct tgaaaatcgg agtcagaaag attgttttga cccctaattc
     4621 ttttaagtat ggaattttca tggcaatgcc ttgaagatcg ccaccgaaat aattcgtgcg
     4681 tgtcggcgtc gcatcccagg cgacgacatc gataggatca ttgtctttat tgccattatg
     4741 atagcgctcg gggatgatgt ggtaggccag ttgtccagca tagctattcg gaacgatgaa
     4801 gacatcgctt tcattaatgt tttgaaattc aaagtttttc agcggataag aaaaagtcag
     4861 cccttccgga gagtaataat aacttcggtg gccgactttt atttcgaata tgtaattcag
     4921 atagcggatg acatcgctga agccgagatt gacggtgtaa tacttgctgg tgccaaattt
     4981 tgcgaccgcg agcattcttt tactcttttt agtaatcttt ctgaatcttt tccaatatac
     5041 taggcgaata tcgtaattgc cgggaaaggc aaattcgact tttatgcata cttctcggcg
     5101 cgataccggg taaatatatt ctttcgtctc cttgtggagc ggggccagtg gtttcatgtc
     5161 aagactcttt tttcctagtt gtttctctta attcgattgc cgtgccgatg aggataccct
     5221 gatttttaag gcttgtgtgc tcaatttggt caaaaagcaa tttagcgctt tgatagccga
     5281 gttcaaagac gtcgacatcg atggtcgtga tgctcggaaa ggatagttgg gcaatctggc
     5341 cattatcaaa ggagatcagc gagacctgtt cgggaatttt tttccctaac tcgctaaggg
     5401 cgcggatgac gccgatcgaa atgatttcat caccgcagat gacgccgtcg atatcaaggt
     5461 gctttttgaa caattctcgt gcgcattcat aaccatcttc tttactgtta ccacatgtcg
     5521 taattaattc cttgttaagt tgaatattgt gcgtggcgag cgcggagaga tagccttcgt
     5581 agcgcttttg actaaaaaac tttttcggat tatgaccgag aaaagcaatt ttatgacaac
     5641 cgctttgaag caagtgctgg gcggctgact gaccgccgcg gaaattatcg atatcaaccc
     5701 aggagatggg cgagctgctc accgacggct ggccgatgga gacgaacggg atgttgtgct
     5761 cgcacaattt cgccaccaac tcgtcatcga tgatattcga tggcaaaatg atgccgtcag
     5821 tgcgtttaga tttaacgaga aaatcgatca tattttgctt cttgggagcc gttttgatat
     5881 tggcgacaat tagtgaatac tctttttgat atacatatct ttcgatgccg tgcatctctt
     5941 cataaaagta ggggttttga aacgattgct catcatcgat atccacgagc aaagtgatcg
     6001 tgtaagcttt attgctcgtt aaacttttcg ccgaagtgtt aaaagcaaag ttcaatctgc
     6061 gcgcctcggc ctctacgagt tgcttggttt ttttggaaat agccggatgg tcgttaagag
     6121 cacgggatac agtcgaaaaa gacaagtcga gattcttggc gatgtctttg atggttgcca
     6181 tgtttgctcc ttatgctatc gtttgcacaa aatatgatgg ttaggattta tttaagcgag
     6241 tccaaaggct tgatttctaa tttcataata taatcgctta ataatactgt caagaaagtt
     6301 atgcaatcgt ttgcacaaac attggcttta tgccgctgat gccaccattc cgacataata
     6361 aaaccctcgc gcttcctgca aatggcgagg gtttggatta ccaaatatcc gttgcttatt
     6421 tttttatgcc attaggagcg gcgtaatcaa ataactggta gtactttttc agatgatcgc
     6481 ctctgcgttg ggcgagagcg acaaaaagaa tatcaatgat gttttcctgc actacgcgtg
     6541 agcgagttga aaaactgcga aagcctttgc ctaaagagga atgctgaata ttgatattac
     6601 atgtctttgt cagggtgttt tcaccatatt tcgtaatgga gatgcattta atgcggcgat
     6661 tggcggcaat ctcggcggcg gcgatgatat cttttgtttc gcccgagtaa gaaaggaaga
     6721 tggcggcatc ttcggcattt aaggttgcgg ccgacaaaat ttgtgagtgt ggagtatcgt
     6781 agagacggca attgatgccg agacgatcaa atttgaaaag ggcatcttga gcggcgatta
     6841 aagatccgct tagggcgtag atggcgacgc ggtttgcggt gttgatagca ttgacggcgc
     6901 gttcgacaca cttctcatca acgattttta aggtgctcat caaagcttcg atgttgagct
     6961 tgctgaccat atcgatggtc tggcggatcg tcgactcttt cggcgtctta ttatcgatat
     7021 cataaatgtt atcttcagaa agggtgttgc tgacaacatc atgatagagg ctcttaataa
     7081 aatcgcgata tcctttgaag ccgagcagat tggcaaagcg gataatcgtg gccgtcgaac
     7141 tcttgctttt tttggcgagt tcgctgatag tcatctgaat gacttcttct tggtgatcta
     7201 aaacatagcg ggccactttg ccgacttgtt tggaaaagga atcgagcgat tcgttaattc
     7261 tgacgatgca gttattcatg agcgatctcc cttcgttaat tccaaaaaca acgattaaca
     7321 atgatacaaa agatgaataa aattttcaaa tttattgtac accaattttc aaatttgcat
     7381 ttactttttt aaatactcgc tcctttttgc ccacaaaaaa tggcttttta agacaaaagt
     7441 tggctaatta atcaaatatc tggtgcgtaa aacctgttga tcaaattctg aaactttgag
     7501 ttgattttga atgtattcat caagggagcc atactttgag ttcacggctt taaagtattc
     7561 atcaatgtag tcttttttgg cgatgtagta agcttcatag tacttaattt gtttcttgtc
     7621 tgaagggtcg acagtcttta atttctcgtc gagaatcggc aaggtgattt cgttggtgcg
     7681 aaaatgctgc tgataaatcg tttccatatc gcagcctaaa atccttaaga gaagggcggc
     7741 ggcgatgccg gtgcgatctt ttcctgatgt gcagtgccag aggatggcgc cctcgtcatt
     7801 ttcgatgacg atgcgaaaga atttgcggta cgcttccatg gcttcttcct gaagcgccag
     7861 tgagcgatag acgcccatga aaaattcatc gggatcgatg gtaatctcct gattataaga
     7921 attagtctct aaaaacttga gcgtgtactt atgataatac ttgatggtgt catcgattaa
     7981 atccctgcga tgaatgctcg agttcgtcga acgaaaatcg atgaccaccc gcaggttata
     8041 tttgtttttt agaatatcta actcttttgc ggaaagctta tgcaggtcac tgctgcggag
     8101 cagacggcct aagacgatgg tgtgcccgtc tgcggtttta atgccaccta ggtcacggat
     8161 gttatcgatg gttttaaaat ttaaaaacat gttttggccc tttccctcac tggtttaact
     8221 ccttcggcgt gcttcgctcg ggaatgacta tgtcgcggtt tgagacaata tatgtcctcg
     8281 ccacatagtc atgcgggccc gagaaggtct tggtcaaaga cattaaaaca atcgagataa
     8341 aaatggggat taaagtaaag taggaaaaac gggcgaacag aagaatcggc acccagatgt
     8401 taaataaaag caagatgaca aagcggatag cgacctgccc ttttttgact ctcgtgtcat
     8461 cgcgggcatc ggcgagacat agtttaaaaa cgagcttaaa gggcgtctgg ccgtttttca
     8521 ttagaagcgg aatgagaaga aaaatgattg ccatgccaag cgtcgcgctg aaaaggctcg
     8581 taaaaatgac gatgtaaaaa gttttctcca ctccgttgag atatactggg tccgtttcca
     8641 aaaaagtgac ggcatcatca tatgcgccgg caaagaaagt tttgacttcc gcatcatcgg
     8701 ctccatcagc ccttacaaaa acgccttcgc tttcttcgac aaaatacccg gagacgagtt
     8761 ttgagttatg atagcgagcc aggcggttat cggcgatggc gcggacgtca ttggtgtaat
     8821 aatatgttag gggaacatca tagttttctt cgggactgag gttttcatca tatgcttccg
     8881 tgatggtcaa taaacctttg tcatcatcaa gaacatagag atgtgagtct tggaggaccg
     8941 cttgcgattc gtccatgagt tgatgatagc ccagcggttc aaaaatggtg aaatataagg
     9001 cggcttcaat cccggcaaaa aggagacagg ccagcaaaaa atccaagaaa gaagccgcaa
     9061 ttcgcgtcag taaatacggt ttaggcaggt ttttcttggg tgtttcgacg atttcgaccg
     9121 tctcattttt attgttcatc ttagatattt aaatatatca aaagcaggcg cttattgata
     9181 acgaaagaag aaatcggatc tttatttaag aacttcgcaa gcgcctaaag cgatgctgac
     9241 tttggattct tcatcaaaga ggacgatgct tttaccgatg atgtcaaaac tgatcttctg
     9301 gtcaaacgca tagtcgaccg caccgaactg cacggcggat agaattgtat ccttcaggtt
     9361 aattttaacg gtcgtttcca tgccggaggg aagggtcgaa taagcgagac cttcaatcgc
     9421 cccattatct ttaattaaaa ggaattccgg gcggatacca atgatgacat tttcgcttgt
     9481 aagtttgact ggttcgttgc tcgtatattt tatattgagg cctaagactt caagatttat
     9541 cacagtatca gaaaccttct tggcttttgc tttcaagaag ttcattgtcg gactgccgac
     9601 aaaatcagcg acaaacaaat tcgtcgggtg attataaacc cttaagggct tatcatattg
     9661 ctgtaaaaga cctttgttaa tcaggcaggt tctgctggca agggtcatcg cttcgagctg
     9721 gtcatgcgtg acgtaaacaa aggtcgaatc agtatcgcga tgtagtcttt ttagttctgt
     9781 tctcatctct ccgcgaagct tcgcgtcaag gttagagagg ggctcgtcca taaacaacac
     9841 ttgcggatta ggagccaggg tgcgggcgat ggcgacacgc tgctgctgtc cgccgcttaa
     9901 ttcgctggga tagcgatctc tgaattcgga aatctgcagc gttttcataa cttcttcgac
     9961 tcgggcatta atctgctctt ttttccattt caagttttca aggccaaacg aaatattctt
    10021 atagacggtc atgtgcggcc acagagcgta gttttgaaac aagaagccga tgtcgcggtt
    10081 agaaggcgaa acattaattc ctttatcgct atcaaagacg acacggtcgc cgatgatgat
    10141 ttttccggaa gtcggagttt ctaagccggc aatcatgcgc agcgtcgtcg tcttgccaca
    10201 gccggaagga ccgagcaaag tgacgaattc gcggtcttca ataatcatgc tgagattatc
    10261 gacggcgaca aattttcccc attttttcgt gacatttatc aattcaattc gtggcataac
    10321 tattagcctc ctataccttt atcaatcgag gcgccggtta atttattaat taagaaattg
    10381 acgccgatga cgaagacgac gattaacaag ttgacggcat tactcatctg agtcatgcca
    10441 tagacattat agaattgtaa gacattggtg attagtgaga agctcgaagt gatcaaaaca
    10501 aagagtgtta attcgcgcat acaagagata aacggcagta aatatccgct gattaaactc
    10561 gatttctgaa taggaaacaa cacatgtgtc atacgcttcc accacgggca gccggtaatg
    10621 atggcggcct cttctatttc gccggacacc tggagcatgg cattcgtgcc gcttcgcgat
    10681 gcgaacggta agaacttaat cgaggcgacg agaatgcaga ttgccaccgc cgaccattca
    10741 ttattcgtac ttgcgacgtt gagccactgg aagccttgct taaaggtgag agaaaagaag
    10801 atggcgccaa agctgagggc cggaataaga taaggtaaaa aggccgtgct cgaaacataa
    10861 ttggcgatgc ggttacggcg gtttttgctg acggcatagc cgattaaaat gccgaatgta
    10921 ccagccacca atgcgaccac cgccgccact aggacactgc ggccaaaggc acgccaaatc
    10981 gtgtagttgt ggaagatg
//
LOCUS       DAOSSL010000031        10901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5902_length_10901_cov_15.618938, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000031 DAOSSL010000000
VERSION     DAOSSL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10901
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5902_length_10901_cov_15.618938"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="rsmD"
                     /locus_tag="PLH17_03735"
     CDS             complement(<1..377)
                     /gene="rsmD"
                     /locus_tag="PLH17_03735"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOR20913.1"
                     /translation="MIRIIGGKHRSRLIETPPSEIVLPTKNMVREALFSILGRKVSNA
                     IVLDLFAGSGALGLEALSRGANKVYFSDHHSEPIKIIGKNVESLKEKDNVEVHLCDYS
                     EMIDYLERNSVLLDIVFVDPPYAS"
     gene            complement(374..736)
                     /locus_tag="PLH17_03740"
     CDS             complement(374..736)
                     /locus_tag="PLH17_03740"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOR20914.1"
                     /translation="MNAILLNKINDLKDALASDERILALGRAEKAMEEDEEVMRLAYQ
                     KDQAETAFNDALRHFGESSTEVKSAQKNLFAAKSILEIHPLVRAYLLAYREVRILYEE
                     INEKLFAPFREHICEVHQ"
     gene            794..1342
                     /gene="def"
                     /locus_tag="PLH17_03745"
     CDS             794..1342
                     /gene="def"
                     /locus_tag="PLH17_03745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOR20915.1"
                     /translation="MFKIVKDSSLSLRSKSKPVEMPLTKKDRDLILRMFSYLKKTQDP
                     AFREKHPSIREGVGLAAPQVGVNKRMTTIYYHDSEGKEIQYALVNPRIIENSIKKCYL
                     LAGEGCLSVDEKHEGYVPRDNKIKVRAYDVLTEADIEISARGYDAIVLQHEIDHLDGI
                     LYYDRINKNNPFQVIEGAVEIS"
     gene            1436..3106
                     /locus_tag="PLH17_03750"
     CDS             1436..3106
                     /locus_tag="PLH17_03750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOR20916.1"
                     /translation="MYHSISSGISVYALGGLGEVGKNMYVVESESTIIIIDSGVMFPG
                     AEMPGVDYVIPDYTHLKNNRGKIRALFITHGHEDHIGGIPFLIRTVHIPVIYAPKLAA
                     ALIRHKLEDMRIKEPVRIVEYDSDAAFHIDDFVVTFFRVTHSIPDAYGICIDSPDGRI
                     VHTGDFKIDLTPVGPDIELDKIARIGSEGVDLLLSDSTNAEIEGYTPSETSIIAGINE
                     IFGKADGRLIVSTFSSNISRIQQLIEAAIRHNRKIAIVGRSMENAVTASRDMGYIKIS
                     DDNIVDLDNINNYKPSELCILCTGSQGEPMAALARIANGEHKSLRISPGDTVVFSSSV
                     IPGNGVLIDRIVNQLVRRGANVLTNSILLNIHSSGHPAKQELRLMLKMINPRYFMPIH
                     GEYRMLKLHTDIAVDLGMDRNNTFVMANGDTIVLRRHRVTVGPRVPADALYIDGRDIN
                     GLSTAVIHDREILKDDGMVAVLISIDSKANRLIVPPVIHTRGFVLANHSKLIAEGEKI
                     ITDELQKVMANKVTFNEIKNTIRNTLSKYLFAQTERNPMIIPVIMNLN"
     gene            3108..3644
                     /locus_tag="PLH17_03755"
     CDS             3108..3644
                     /locus_tag="PLH17_03755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOR20917.1"
                     /translation="MLILASKSPRRKDLLSKICSDFVIINPDINEDESRQHPHEHPLD
                     VARLKAYRIFQSHPEDNVLACDTVVIIDGEILGKPRDEKEAFQMLKKLSGKRHIVLSG
                     YTFINRNREINRTVRTEVYFRALDDDLIRRYIKTGSPLDKAGAYGIQDDFALVERIVG
                     SYDNVMGFPSEDIAKHCF"
     gene            complement(3646..4014)
                     /locus_tag="PLH17_03760"
     CDS             complement(3646..4014)
                     /locus_tag="PLH17_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HOR20918.1"
                     /translation="MIFLIENLFNKFLKDNALWIALCFAVVIIVTLLTIFLSGKRSGI
                     KSGNKSFASEDFLAALGGRENIISLYAKGSRLSLVLKENNLLVEEDLRKNGVMSIIKM
                     SSKITLVVNGSAEELAKVIK"
     gene            complement(4011..4454)
                     /gene="spx"
                     /locus_tag="PLH17_03765"
     CDS             complement(4011..4454)
                     /gene="spx"
                     /locus_tag="PLH17_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HOR20919.1"
                     /translation="MIKIYTSPSCSSCRKVKKWFEEQQIPFEERNIFNAALDPVELKE
                     ILFKSENGTEDIISERSKIVKEKKVNVEDMTISEMISFIRENPSVLKRPIVVNDRRIQ
                     VGYNEEEIRSFIPQARRIFEKHCNADECPDFNACPHARDCVIDKK"
     gene            4539..5573
                     /gene="trpS"
                     /locus_tag="PLH17_03770"
     CDS             4539..5573
                     /gene="trpS"
                     /locus_tag="PLH17_03770"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018933348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOR20920.1"
                     /translation="MRIRTYNSKDLNGGKDMNRVLSGIKPTGQLTLGNYLGALKHFKK
                     FQDAGEVFVFIADLHALTLPIEPETLRTNTRDLVAFYLASGLDPKRATIFLQSHVSAH
                     SELNTIFQNYLYMGELSRMTQYKDLSYKMNNNAIGLGLFAYPVLMAADILLYDAAIVP
                     VGDDQIQHVELARDLVNRFNKRYGDILVMPKAQVNKVGARIMSLSDPMRKMSKSDSKG
                     DIFLKDDLATIRKKIMASVTDSGSEVIYDLNNKPGISNLLTIYAATKDISIAEVEKYF
                     SGYRYGDFKKAVADAVIEELSPFKERYEQIIQGDEIEKVLKDGASRARIIAEATLKRV
                     KNAIGLLDVK"
     gene            5563..6390
                     /locus_tag="PLH17_03775"
     CDS             5563..6390
                     /locus_tag="PLH17_03775"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOR20921.1"
                     /translation="MLNKRPLLIAVDMDGTLLNTDKVITPRTKRLLRKLSARGHLVVL
                     ASGRPSRALYRYYDELKLNSPLVCYNGAFVFHPLDDAFPRVEFEFPKEVVKELFNKLK
                     PYVKNIMCENDTDIWIYSEDLYLAKFFWYEGMNMHYGDLEETLDVNPMTMILQTNKDN
                     LDEQSIRRIVGQFPQLDVRFWFGLSYFEIFYKTVSKGSALTQIANYYGIPKEDIIAFG
                     DAENDIDMFAVSGISVAMKNGGPDIIKNAHRVSVKDNDNEGIYHTLKQILKEVKNRR"
     gene            complement(6430..7668)
                     /locus_tag="PLH17_03780"
     CDS             complement(6430..7668)
                     /locus_tag="PLH17_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOR20922.1"
                     /translation="MEYLNITLILGLAMAAGLLSTRLMKLLNLPNVTGYLIIGLLLGP
                     FVGHLFSEQAISDFSILSDIALGFIAFSIGCEFKLSSLKKIGGKVVVITLFQALLTAV
                     VVALAVFAFSRDVALSLTLGAIATATAPAATLMVVRQYKAKGPVVDTLLPVVAFDDAI
                     GLIVFSVLLSISKVVATNSSLTAEAILLAPLVEIFLSLAIGAALGALLALVMRFFKSR
                     ANRLTLMITTVFLGVGLTKLFAFIDWYSLSSLLLCMMIGAVFANMRSDSNLVVEGVDR
                     WTPPLFMFFFILSGAELDVRVIVTVGLIGVVYIVARSVGKYFGAYLGALSTKSDKNIR
                     HYLGFALLPQAGVAIGMAKVVSEEMPSDIGVKIVTVVLCSVLFFELVGPIITKLALMR
                     AGEIIKEPKKPKKITPPPAS"
     gene            complement(7668..8000)
                     /locus_tag="PLH17_03785"
     CDS             complement(7668..8000)
                     /locus_tag="PLH17_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20923.1"
                     /translation="MKHLLVVILKNNERAHRLFEKLTELNIKGTVLPSTGLNHALMES
                     NVNHPPIFGGSRYLVEQEYESNATVLIVIDDAKLEIARSTIKEITQNLTDAFMFAVPL
                     NHYEGSDV"
     gene            complement(8056..9756)
                     /locus_tag="PLH17_03790"
     CDS             complement(8056..9756)
                     /locus_tag="PLH17_03790"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOR20924.1"
                     /translation="MNTQQNFSRWLESPKVSPAMKEEMRKMSPQEVDESFFKNIEFGT
                     AGMRGILGPGTNRMNFFTIQRAAVGFALYLFETFPKAKELGVVISHDNRRMSREFTMQ
                     IADIFSKMGLKSYIFDALRPTPELSFAVRYKKACGGIMITASHNPKEYNGFKVYDQHG
                     CQLVPAKIAKLIAIIDRLPDELSLEIPPSLNRGEIIVLDSDVDEEYIRLVKEIQLNPA
                     LPKRGFKIVYTPNHGTSYVNAMRVFKDCGYEVYPVLSQVDPDPDFKGTLSPNPEEKNA
                     YIEPIKLAQEIGAHFIVMTDPDGDRVGVSYLAQSGEYELFTGNQSAALLIDYLLGERK
                     KKGLLAKNGVFYDTIVTSDLGRTIAKSYGLKVETFLTGFKFIGDRIQYYLEHGGPTFE
                     FGYEESYGCLIAPFARDKDGIQAILMYCEMALFHHLRGKTLDVVYDELQQKYGYRLDL
                     SYSVEFKGSAGMQKMRDIMTRLSNRQIKEVLGQRVVEIRDYINSVTRSEKGEAPIDLP
                     KADVVILLLEDGSTISVRPSGTEPKCKFYFGIKGTNAAAIENRPKVLFADLKRQLGIV
                     "
     gene            complement(9753..10736)
                     /locus_tag="PLH17_03795"
     CDS             complement(9753..10736)
                     /locus_tag="PLH17_03795"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOR20925.1"
                     /translation="MIEIKISSQEANQRIDKYVRKYLNNAPLSFIYRIFRKKDVKIDG
                     HWVDINYILKPDDVLRIYVTNDQIRDLSKPLEIKNTVISDAIIYEDANIIIVNKSKGL
                     LVHGDASEKTHTLANQVINYLIQKGEYNPKETKGFVPAPAHRLDRNTSGLIIFAKNLP
                     ALQELEAMFKCRQGIEKHYLALAVGRVLNDQTIDLPLKKDAKAKMVKVDKIVYGAQEA
                     RTKIKVIKYYGDYSLLDVELLTGRTHQIRVHLAAIHHPLVGDGKYGDFKDNRLYKEKF
                     NYDSQFLHAYKLVFSIDKGLLSYLDRRQFTSDLPKREEEILQQLLNKENIR"
     gene            10810..10900
                     /locus_tag="PLH17_03800"
     tRNA            10810..10900
                     /locus_tag="PLH17_03800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10844..10846,aa:Ser,seq:gga)
BASE COUNT         2996 a         2548 c         2442 g         2915 t
ORIGIN      
        1 gaggcatagg gcggatcgac aaagacgata tcgagcaaaa cactatttct ctcaaggtag
       61 tcaatcatct cgctatagtc gcaaagatgg acttcgacat tatctttttc tttgagcgac
      121 tcaacatttt tgccgatgat ttttatcggt tcactatgat gatccgaaaa gtatacttta
      181 ttcgctccgc gcgaaagggc ttcgaggccg agggcgccgc tgccggcaaa taaatctaaa
      241 acgatggcat tgctgacttt tctgcccagg attgaaaaaa gggcttcgcg aaccatattc
      301 ttggtgggaa gaacaatttc gctcggtggg gtttcaatga ggcgggagcg atgcttgcca
      361 ccgatgatgc gtatcattga tggacctcac aaatatgttc gcgaaaaggc gcaaatagtt
      421 tctcattaat ttcttcgtat aatatgcgaa cctcgcgata ggcgagcaaa taggcgcgga
      481 caaggggatg gatttctaat atgctcttag cggcaaaaag atttttctgg gcggatttaa
      541 cttcagttga tgattcgcca aaatggcgta gtgcatcatt aaaggctgtt tcggcctgat
      601 ctttttgata ggcaaggcgc attacttctt cgtcttcttc catcgctttt tcagcgcggc
      661 caagcgccaa aatccgctca tcgcttgcga gagcatcttt taaatcattg attttgttaa
      721 gcagtatcgc attcataagt atcataatag caaatcgcga ttaatcatgg tacaatcttt
      781 aaaagtaaag attatgttta aaatagttaa agattcatcc ctttccctac gcagcaaatc
      841 aaaaccggtc gaaatgcctt tgacaaagaa agaccgcgac ttgattcttc gcatgttttc
      901 atatcttaaa aaaactcagg acccggcttt tcgcgaaaag catccatcca ttcgcgaagg
      961 cgtcggtctt gccgctccgc aggtcggcgt caataagcgg atgaccacca tctattacca
     1021 cgatagcgaa ggcaaagaaa ttcaatatgc tctcgtcaat ccccgcatca tcgaaaacag
     1081 cattaaaaaa tgctatttgc tcgccggtga gggctgtctt tccgtcgacg aaaaacatga
     1141 aggctatgtc ccgcgcgata ataagataaa agtcagggct tacgatgtct tgactgaagc
     1201 ggacatcgaa atttccgccc gcggatacga tgcgattgtt ttacagcatg aaatcgatca
     1261 tctcgacggc attttatatt acgaccgcat caataaaaat aatccctttc aagttatcga
     1321 gggtgcggtg gaaatcagtt gaattaattt tcatactttt atataataac tttgcttaat
     1381 tttgaaaacg ctatttataa ttgcttaatt tttacatcta tttaggagat ttaatatgta
     1441 tcattctatt tcaagtggca tatcggttta tgctttgggc ggactcggcg aagtcggtaa
     1501 aaatatgtat gtcgtcgaaa gcgagtccac gattattatc atcgactccg gcgtcatgtt
     1561 tcccggcgcg gaaatgcccg gcgtcgacta tgtcatcccc gactacaccc accttaaaaa
     1621 caatcgcgga aaaattcgcg ccctcttcat aacccacggt catgaagacc atatcggagg
     1681 gatacccttt ttaatccgga ccgtgcacat acccgttatc tatgcgccta aattggccgc
     1741 ggctctgatc agacacaaac tcgaagacat gcgtatcaaa gaacccgtcc gcatcgtcga
     1801 atacgatagc gacgccgcat tccacatcga cgattttgtc gtcacctttt tccgcgtcac
     1861 ccactcaatt cccgatgcct atggcatctg cattgattct cccgatgggc gcattgttca
     1921 taccggcgat tttaaaatcg acttaacccc cgtcggacca gatattgaac tcgacaaaat
     1981 cgcccgcatc ggctctgaag gagtcgacct tttattaagc gactccacca atgccgaaat
     2041 cgaaggctac acgccgagcg agacgagcat catcgccggc atcaacgaga ttttcggaaa
     2101 agccgacggc cgtctgatcg tctcgacttt ctcaagcaat atctcgcgca ttcagcagct
     2161 catcgaagcc gcgattcgac acaatcgcaa aatcgcaatc gtcgggcgca gcatggagaa
     2221 tgccgtcacg gcatcacgcg acatgggcta tatcaagatc agcgatgaca atatcgtcga
     2281 ccttgacaac atcaataatt acaagccgag cgaactatgc attttatgca ctggttcaca
     2341 aggcgaacca atggcggctt tggcgcgaat cgcaaacggc gaacacaaat cgcttcgcat
     2401 cagccctggg gataccgtcg tcttctcctc atcggtcatt cccggaaacg gcgtcttaat
     2461 cgaccgcatc gtcaatcagc tcgtccgccg cggcgcgaat gtcttaacca attctattct
     2521 tttaaatatt cactcttcgg gccaccccgc caagcaggaa ctccgcttga tgctcaagat
     2581 gattaatccg cgctatttta tgccgattca tggcgagtac cgcatgctca aactccatac
     2641 cgacatcgcc gtcgatttag gcatggaccg caacaacaca tttgtgatgg cgaatggcga
     2701 cacgattgtt ttaagaaggc atcgcgtcac tgtcggaccg cgcgtgccgg ctgatgctct
     2761 ctacatcgac ggacgtgata tcaacggcct cagcaccgcc gtcattcacg accgcgaaat
     2821 tttaaaagac gatggtatgg tcgccgtctt aattagcatt gattcaaaag ctaatcgcct
     2881 catcgtcccg ccagttattc acacgcgcgg ctttgtttta gccaaccatt cgaagctgat
     2941 tgccgaagga gaaaaaatca tcaccgatga acttcaaaaa gtcatggcaa ataaggtgac
     3001 ttttaatgag atcaaaaata ccattcgcaa cactttaagc aaatacctgt ttgcccagac
     3061 cgaacgcaat ccgatgatta ttccggtcat catgaatctc aattaagatg ctgatactcg
     3121 cttccaaatc tccccgtaga aaagaccttt taagcaaaat ttgcagtgat tttgtcatta
     3181 ttaaccccga tatcaacgaa gatgaaagcc gtcagcatcc gcacgaacat cctctcgatg
     3241 tcgcgcgttt gaaggcgtat cgcatttttc agagccatcc cgaggataat gttttagctt
     3301 gcgatacggt cgtcataatc gatggtgaaa tcctcggcaa accgcgcgac gaaaaagaag
     3361 cttttcaaat gcttaaaaaa ctatctggta aaaggcatat cgttttaagc ggttatactt
     3421 tcatcaatag aaatcgcgag attaaccgga ccgtcagaac cgaggtctat ttccgcgccc
     3481 tggatgatga tttgattcgc cgctacataa aaaccggctc gccattagac aaggccggcg
     3541 cctacgggat tcaagatgat tttgctctcg tcgagcgcat cgtcggaagc tatgataacg
     3601 tcatgggttt tcccagcgaa gacatcgcca agcactgttt ttaaactatt ttatcacttt
     3661 agctaattct tctgccgaac cgttgacaac taatgtaatc ttgctcgaca ttttaattat
     3721 cgacataacg ccattttttc ttaaatcttc ttcgacaagg agattatttt cttttaagac
     3781 aaggctcaaa cgcgaacctt tcgcatatag ggaaatgatg ttttcacgac ctcccagcgc
     3841 cgctaaaaaa tcctcgctgg caaaagattt atttccgctt ttaataccgc ttctttttcc
     3901 ggataaaaaa atagtgagga gggtaacgat gattacaacc gcaaaacaaa gcgcgatcca
     3961 taaggcgtta tcttttaaaa atttattaaa gagattttcg attaaaaaaa tcatttcttg
     4021 tcaatgacgc aatcgcgggc gtgtgggcaa gcgttgaaat cggggcattc atcggcattg
     4081 caatgctttt caaaaatgcg gcgagcctgc ggaatgaagg aacggatttc ttcttcgtta
     4141 tagccgactt ggatacggcg atcgttaacg acaattggac gctttaaaac ggaagggttt
     4201 tcacgaataa agctgatcat ctcggaaatc gtcatatctt caacgttgac tttcttctct
     4261 ttgacgattt tactgcgttc cgaaatgata tcttcggtcc cattttccga tttgaataag
     4321 atttctttta attcgactgg atcgagggcg gcattaaaaa tattcctctc ttcaaagggt
     4381 atttgttgtt cttcgaacca ttttttgact ttgcggcaac tggagcagct gggggatgtg
     4441 tatattttta tcataatttc taactgtcat tctatcataa aagaccatta gagtagatag
     4501 ttttttggga tgatagccgt attggctaaa ttgacactat gcggattcgc acatataata
     4561 gtaaagactt aaacggagga aaagacatga accgcgtttt aagtggcatt aaaccgacag
     4621 gacaactgac gctcggcaac tatcttggcg ccctaaagca ctttaaaaag tttcaggatg
     4681 cgggcgaagt tttcgtcttc atcgccgacc ttcatgccct gactcttccg atcgaaccgg
     4741 aaactctgcg caccaacact cgcgatttag ttgcttttta tctggccagc ggccttgatc
     4801 ccaagcgggc gaccatcttt ttgcaaagcc atgtcagcgc gcacagcgaa ctcaatacca
     4861 tctttcagaa ttatctttat atgggcgaac tttcacgcat gacccaatat aaggatttgt
     4921 cttataaaat gaacaataac gccatcggtc taggactctt tgcctacccc gtcttaatgg
     4981 ccgccgatat cctcctttac gatgccgcca tcgtccccgt cggcgatgac caaattcaac
     5041 atgttgaatt ggcgcgcgat ctcgtcaatc gctttaataa gcggtacggc gacattctcg
     5101 tcatgcccaa agcgcaagtc aacaaagtcg gagctcgcat catgtcgctc agcgatccga
     5161 tgcgcaagat gtcaaaatca gattcaaaag gtgacatttt cttaaaagac gatctggcga
     5221 cgattcgcaa aaaaatcatg gcttccgtaa ccgattccgg aagtgaggtc atctatgatc
     5281 tcaataataa acccggcatc tccaatcttt tgacgattta tgcggccaca aaggatattt
     5341 ccatcgctga agtcgaaaaa tatttctccg gctatcgcta cggcgatttt aaaaaggcag
     5401 tcgccgacgc cgtcatcgaa gaattgtcgc cttttaaaga aagatacgag caaattattc
     5461 aaggcgatga aattgaaaaa gtcttgaaag atggcgcctc ccgggcgcga atcatcgccg
     5521 aagctacttt aaaaagagtt aaaaacgcca taggtttgct cgatgttaaa taaaagaccg
     5581 ctcctgattg ccgtcgatat ggacggcacg ttacttaaca ccgacaaagt tatcacgccg
     5641 cggacgaagc ggcttttacg taagctcagc gcccgaggcc atctcgtcgt cctggccagc
     5701 ggccgcccat cgcgggccct ttatcgctat tacgatgagc taaaactcaa ttcaccgctt
     5761 gtctgttata acggcgcctt tgtgtttcat cctttggatg atgctttccc gcgcgtcgaa
     5821 tttgaatttc ccaaagaagt tgttaaagag ttgtttaata aactgaaacc ttacgtcaaa
     5881 aacatcatgt gcgaaaacga caccgacatc tggatttata gcgaggatct ctacctcgcc
     5941 aaattcttct ggtatgaagg tatgaacatg cattatggcg acctcgaaga aaccctcgac
     6001 gtcaatccga tgacgatgat tttgcagaca aacaaagata atctcgatga gcagtccatt
     6061 cgccgcatcg tcggccaatt tccgcagctt gatgtccgct tttggttcgg tctttcctat
     6121 tttgaaatct tttataaaac ggtttctaaa ggatcggctc taactcaaat tgcaaactac
     6181 tacggaattc caaaagaaga catcatcgcc ttcggagatg ccgaaaacga tatcgatatg
     6241 ttcgccgtct ccggcatcag cgtcgccatg aaaaacggcg gtcccgacat cattaaaaac
     6301 gctcaccgcg tcagtgtaaa agacaatgat aatgagggca tatatcacac cctcaaacaa
     6361 atacttaaag aagtaaaaaa tcgccggtag atgaaccctt tacagcgcat ttgctaaaac
     6421 ggcgcttttt taagatgccg gagggggagt tattttttta ggtttcttgg gctctttgat
     6481 gatttcgcca gcgcgcatca aagccagttt ggtaataatc ggtccgacga gttcgaagaa
     6541 caagaccgag cataagacga cggtcacaat tttgacgccg atatccgatg gcatctcttc
     6601 ggagacgact tttgccatac cgatggccac gccggcttga ggcagaagag caaagcccag
     6661 ataatggcgt atatttttgt cgctcttggt cgataaagca ccgaggtagg cgccgaagta
     6721 tttgccgacg cttcgcgcta cgatataaac aacaccgatc aatccgaccg tgacgatgac
     6781 cctaacatcg agttcagcgc cgcttaaaat aaagaaaaac atgaagagag gtggcgtcca
     6841 gcggtcgact ccttcgacga ctaaattcga gtcgctgcgc atattggcaa agacggcgcc
     6901 gatcatcatg caaagcaata gcgacgagag agaataccaa tcgataaagg cgaagagttt
     6961 agttagtcct acgccaagaa aaacggtcgt aatcatcagc gtcaggcgat tggcccgcga
     7021 tttgaaaaag cgcatgacaa gagctagtaa agcgccgagt gcggcgccga tggccagcga
     7081 taagaaaatc tcgactaacg gagcgagcaa aatggcttcc gccgtcagag aactgttcgt
     7141 ggcgacaacc tttgaaatcg acaacaaaac cgaaaagacg atgaggccga tcgcatcgtc
     7201 aaaggccacc accggaagca aagtatcgac gaccggtcct ttggctttgt attgacggac
     7261 gaccatcaat gtcgcggccg gagccgtcgc cgtcgcgatt gcaccgaggg ttaacgataa
     7321 ggcgacatcg cgtgagaaag caaagacagc caaagcgacg acaaccgctg tcaataaagc
     7381 ttgaaaaagg gtgatgacaa cgactttgcc gccaatcttc ttcaaagaag aaagcttgaa
     7441 ttcacagccg atgctgaaag cgatgaagcc taaagcaatg tcgcttaaaa tagaaaaatc
     7501 gctaatagct tgttcggaaa aaagatgacc gacgaaagga ccgagaagca aaccgatgat
     7561 gagataaccg gtgacattgg gcaaatttaa aagcttcatc aatcttgttg aaagaagccc
     7621 cgcggccatt gccagtccga gaataagagt tatattaaga tattccatta aacgtcgctg
     7681 ccttcgtagt gattaagggg taccgcaaac atgaaagcgt cggtcagatt ttgggtgatt
     7741 tctttaattg tcgagcgggc aatttccaat ttggcgtcgt cgatgacgat taagacggtc
     7801 gcatttgact cgtattcctg ctcgacgaga tagcgcgatc ctccgaagat tggaggatga
     7861 ttaacattgg attccatcaa ggcgtggttt aaacccgtcg agggtaaaac cgtccctttg
     7921 atgttgagct cagtaagctt ttcaaagagg cgatgcgccc gttcgttatt ctttagaatt
     7981 acaaccagta aatgcttcat atttgactcc ttaacaatgt taaacccaat ttgcgttttt
     8041 tcaagaaaaa aaacactaga cgatgccgag ttgccgcttc aagtctgcaa agagaacttt
     8101 tggccgattc tcaatggccg cggcgttcgt tcctttgatg ccaaaataaa atttgcattt
     8161 cggctcggtg ccgctcgggc gaacggagat ggtcgagcca tcttcaagaa gcaaaattac
     8221 gacatcggct tttggtaaat caatcggcgc ttcgcctttt tcgcttcttg tcacgctatt
     8281 gatatagtcg cggatttcaa cgacacgttg ccccagcact tccttaattt gacgattgct
     8341 gagccgcgtc ataatatcgc gcattttctg cattccggcc gaacctttga attcgaccga
     8401 ataggataga tcgaggcgat agccgtattt ttgctgaagt tcgtcataga cgacatcaag
     8461 tgttttgcca cgcaggtgat ggaacaaggc catctcgcaa tacattaaaa tggcttgaat
     8521 gccatcttta tcccgggcaa agggagcgat taaacacccg tagctttctt catagccgaa
     8581 ttcaaatgtc ggaccgccgt gttcaagata atattgaatg cggtcgccga taaacttgaa
     8641 gccggtgaga aaggtttcta cttttaaacc atagctcttg gcgattgtcc gcccgagatc
     8701 tgaggtgacg atcgtatcat agaaaacgcc gtttttagcg agcagtcctt ttttctttcg
     8761 ctcgccgagg agatagtcaa ttaaaagggc cgcggactga ttgccggtaa aaagctcata
     8821 ttcaccgctt tgagcaagat agctgacgcc gacgcggtcg ccgtcaggat cggtcatgac
     8881 gatgaagtgg gcgccgattt cttgggcgag tttaatcggt tcgatataag cattcttctc
     8941 ttcgggattc ggcgaaagag tacctttaaa atcgggatcg gggtcaactt gcgaaagaac
     9001 gggataaact tcatagccgc agtctttaaa aacgcgcatg gcattgacat aacttgtgcc
     9061 atggttgggg gtatagacaa tcttgaaacc tcttttcggc aaggcgggat tgagctgtat
     9121 ttctttaaca agtcgaatat attcctcatc gacatcacta tctagaacaa taatctctcc
     9181 gcggtttaga ctcggcggta tctcgagcga taattcgtcg ggaaggcggt cgataatcgc
     9241 gatcagtttt gcaatttttg ccggcaccaa ctggcagccg tgttgatcgt acactttaaa
     9301 tccgttatat tctttggggt tgtgtgaggc ggtgatcatg atgccgccgc aggctttctt
     9361 atagcgcacg gcaaaagata attccggcgt cgggcgaaga gcatcaaaaa tgtatgattt
     9421 taagcccatt ttcgaaaaaa tatcggcaat ttgcatcgta aattcgcgag acatacgccg
     9481 attatcgtgt gagataacga ctcccaactc ttttgctttc ggaaaggttt caaatagata
     9541 aagggcaaat cccactgccg ctctttgaat tgtaaaaaag ttcatgcgat ttgttccggg
     9601 gccgagaatt ccgcgcatgc cggccgtgcc gaattcgatg tttttaaaaa acgactcatc
     9661 gacttcttgc ggactcattt ttctcatctc ctctttcatg gccgggctga ctttcggact
     9721 ttcaagccag cgggaaaagt tttgctgcgt gttcatctga tgttctcctt gtttaataat
     9781 tgctgaagta tttcctcttc acgtttcgga agatcactcg taaactgccg cctgtcgaga
     9841 taagaaagaa ggcccttatc gatgctaaaa acgagcttat aggcgtgcag aaattgcgag
     9901 tcgtaattaa acttttcttt gtagaggcga ttatctttga aatcgccgta ttttccatcg
     9961 ccgaccaggg gatgatgaat ggcggcgaga tgcacgcgaa tctggtgcgt ccttcccgtt
    10021 aacagctcca catcgagaag cgagtaatcg ccataatatt tgatgacttt gatttttgtg
    10081 cgcgcttcct gggcgccgta aactattttg tcaactttaa ccatcttggc ttttgcgtct
    10141 tttttgagtg gtaaatcgat tgtttggtcg ttaaggactc ggcccaccgc caacgctaaa
    10201 taatgttttt cgataccctg acggcattta aacatcgctt ccagttcctg caaggccgga
    10261 agattcttag cgaaaataat taggcccgaa gtattgcggt cgaggcgatg ggcgggagcc
    10321 gggacaaaac ctttggtttc tttaggatta tattcgcctt tctgaatcag ataattgatg
    10381 acttgattgg cgagggtgtg cgttttttcg cttgcgtcgc catggactaa aagacccttg
    10441 cttttattga cgatgataat atttgcatcc tcgtagataa tggcatcgga aatcaccgta
    10501 ttttttattt cgagaggctt agacaagtcg cgaatttgat cattggtgac atagatgcga
    10561 agcacgtcat cgggcttgag gatgtaattg atatcgaccc agtgaccgtc aatcttgaca
    10621 tccttctttc gaaagatacg atagataaaa ctaagaggtg cgttatttaa atacttgcgg
    10681 acatatttgt cgattcgttg gttggcctct tggctcgaaa ttttgatttc aatcatattt
    10741 aataatatta tatattatgc tttaatcgtt gtaattagcg ttatgtttac ttataatttt
    10801 tcttgtcgcg gaggaatacc caagcggctg aaggggcagg cttggaaagc ttgtaggctc
    10861 ttaacggggc gcgagggttc aaatccctct tcctccgcca t
//
LOCUS       DAOSSL010000032        10887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_5914_length_10887_cov_17.366322, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000032 DAOSSL010000000
VERSION     DAOSSL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5914_length_10887_cov_17.366322"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..994
                     /locus_tag="PLH17_03805"
     CDS             59..994
                     /locus_tag="PLH17_03805"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOR20926.1"
                     /translation="MSLLTKRETLVQNITYMAIMAAINAVFSLLAALVPALSIFLMIV
                     LPLSSAIVFLFCKHKYFPIYAVATILICLLVTIFDMSFTIFYVIPSILTGYLFGLFIK
                     YKLHAVWIILITSIAQGLFTALSIPLIQWIFDVNIIDTFKGLLQVTASVNIDIIVPSF
                     LFYLALTQMVFSYIVIQSEINKFGYEISDQPINTTTYSLILFGVIIAIIPFIFLLPSG
                     AYLLLSISLYFMFFIVFTHILQKQYKVLIAFGISLLAFMFLFAFLYPIVPTPFGLLLV
                     SILPALIALISLLNIVLFMRRSKDKMISTGKETKE"
     gene            991..1383
                     /locus_tag="PLH17_03810"
     CDS             991..1383
                     /locus_tag="PLH17_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20927.1"
                     /translation="MIKFLQIFGQGILYVVLSPFFLVILAFYVVYTALVMIFMFLKMI
                     ILFFAGKSVFADLPEDIEAEQMKNKLRDEKLAKENPQTPPLPYPYPPYGYPGYPYPPM
                     PGQVINQPDVKEIDAKPIDVEEEKKDEQ"
     gene            1380..2057
                     /locus_tag="PLH17_03815"
     CDS             1380..2057
                     /locus_tag="PLH17_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20928.1"
                     /translation="MIPFLITISENEKRMLLVLLAAVILVFVLIGLLGLLVKRIMSSQ
                     AKRADTFMYDLVYTKAIKNPRHFRRVGFKKNHRLLFQQSWIPFLILLVAAAFLVTYMA
                     IANHWNLNVFDYYGTDGTDGEGFTTLFFIFDFANAPKSTFFGMQIVSDWPPLLSSPYL
                     SANAWPSYVFVPIFLTGAIWYLICVQAFIARTWRILVMSKKSFEKSLDNYDATKNPID
                     NPLPPLQ"
     gene            complement(2078..2248)
                     /locus_tag="PLH17_03820"
     CDS             complement(2078..2248)
                     /locus_tag="PLH17_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20929.1"
                     /translation="MVDIQEKTQEELAEIKKRQKALSSRRFFVLFIIIDILLVGVVLY
                     EMISIAMQAFNS"
     gene            complement(2255..>2650)
                     /locus_tag="PLH17_03825"
     CDS             complement(2255..>2650)
                     /locus_tag="PLH17_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOR20930.1"
                     /translation="MDLQSEHERYITEKVVNGPVFLIDYPKDLKAFYMYQNDDGKTVA
                     ACDLLVPYVGELVGGSQREDRYDILKDKMEALHLLPDLQWYLDLRKYGGCPHAGFGIG
                     FDRLLMYITGINNIRDVQPYPRSANAIKY"
     assembly_gap    2651..2750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2751..3810)
                     /gene="asnS"
                     /locus_tag="PLH17_03830"
     CDS             complement(<2751..3810)
                     /gene="asnS"
                     /locus_tag="PLH17_03830"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOR20931.1"
                     /translation="MEPLSITVYDLYVITYGDDEEFKDKLKQGVYLEGWVKSNRDNKA
                     VGFIDFNDGTNFKGIQLVYSRDKLNNYDSISKIKTGSSIRVMGDFVLTPEAKQPFEIH
                     VKELDLVGECADDFPLQKKYHSVEFLRDIAHLRARTNTFSAVFRVRNVLTMAIHTFFQ
                     EQNFMYVHTPIITGSDAEGAGATFSVVTGPKKSNEFFGKDVALTVSGQLHVEAFAMAF
                     RDVYTFGPTFRAENSNTPRHASEFWMIEPEIAFADLGDDMELIEACIKYCIEYVLDRC
                     PLEMEFFNNILDQSHTLLDRLNTLLESDFKKMTYTEAIDILLKAVKDGVKFDNADIVW
                     GMDLQSEHERYITEKVVNG"
     gene            complement(3873..4883)
                     /gene="tsaD"
                     /locus_tag="PLH17_03835"
     CDS             complement(3873..4883)
                     /gene="tsaD"
                     /locus_tag="PLH17_03835"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOR20932.1"
                     /translation="MALILAIESSCDETAVAITRDGTLLSNVVATQIEMHKKYGGVMP
                     EMAARLHAENITIVLNEALEKANVKLEEVDAFAFTRGPGLIGALHVGMQMAKTLALIY
                     QKPLIPVHHLAAHIYANEYIKPFQFPMLCTVVSGGNTELVYMPRHLEFEVVGETKDDA
                     VGECFDKVARVLGLPYPGGLPIDRNAKLGKPTYKLPTPLRNGGFDFSYSGLKTAVVNL
                     VNSLNQKDEEINIFDMCKSFQDIAIGMVVDKTIAAAKHYQVKQVVLAGGVSANSYLRE
                     QIGNYFAGTDINVIIPPLWATTDNAAMIAKLADHLYERKIFANMDIGVDPNWRISDYD
                     KF"
     gene            complement(4885..5328)
                     /gene="rimI"
                     /locus_tag="PLH17_03840"
     CDS             complement(4885..5328)
                     /gene="rimI"
                     /locus_tag="PLH17_03840"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOR20933.1"
                     /translation="MENADLDRVLEIEEESFVTPWTRRDWLYELTENPINVIFVLEVI
                     DNGVKEIAGFIDFMVTFSSSTISQIAIAKKYRKQHLGTELLNKMIEHLKNEYNEIIET
                     ITLEVRTHNQAAIAFYEKHGFEGVLIKPAYYSNGEDALYMVRRLI"
     gene            complement(5352..5945)
                     /gene="tsaB"
                     /locus_tag="PLH17_03845"
     CDS             complement(5352..5945)
                     /gene="tsaB"
                     /locus_tag="PLH17_03845"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOR20934.1"
                     /translation="MYSLLLDSANRDLNVGLAKDNQIIDRISYQAWQRQSELMAVEVD
                     AILKRNNISAKDIGEVVVTIGPGSYTGIRIALTIAKTLAFALDIKIYAVSSLISQKAP
                     HQPTISVINARSNRSYVAIYDADGQVLVIEKVWPNDELIAWIKGNADFIVSGDAKYLG
                     IDAYQPSVLDGMLEAKKKSIPVENILTLKPVYLKDLL"
     gene            complement(5938..6405)
                     /gene="tsaE"
                     /locus_tag="PLH17_03850"
     CDS             complement(5938..6405)
                     /gene="tsaE"
                     /locus_tag="PLH17_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOR20935.1"
                     /translation="MKYIFISKSSEDTKRVAGRLAGFLFPGAVITLDGPLGSGKTTFV
                     GGVAKGLDILERVISPTFNIMKCYFEARLPMFHIDAYRLENRDSELGLEEFIEGEGVT
                     LIEWPDYIKPLIPASHLRIVIEALGENSRRITFENIGSSYEQVIKSLKETENV"
     gene            6475..7368
                     /locus_tag="PLH17_03855"
     CDS             6475..7368
                     /locus_tag="PLH17_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR20936.1"
                     /translation="MLKVIVIGAGPIGLYLAASLKKANISHLVLEAGEKAGGQLTALY
                     PEKEIVDLPGIPSIIAKDYIALLAKDVDLNNFRFRQEVIDIKSFEDHVEVIAKEDHYQ
                     AEYIIIATGLGFYKPKPMGLARENEFANILYSLSSFDFLKGKRVAIFGGGDSALDWAK
                     EISRVTNSIHLIHRRREFRGNFETIKDIKEIKIHTPYVPSQLIIEDNHLLGVRIKHAE
                     EESYVDLFVDYILVNFGNVPSPVAFSVPKQNTGIICDEYRCVLNRVYVAGDAIGVPGK
                     IKRIGDGLKDADSVLKTILKF"
     gene            complement(7358..7900)
                     /locus_tag="PLH17_03860"
     CDS             complement(7358..7900)
                     /locus_tag="PLH17_03860"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOR20937.1"
                     /translation="MNYEGRWLRIQAYKHNGELHRAWSHGFVLEDNDRHIILISVRSS
                     VVEHDGRKWHTREPAVFIFFKQEWMNVIAMLKDEGQDISYYVNIASPAVIHANCIKYI
                     DYDLDIKLFPGGSTKLLDQNEYERHAYSLDYSEDIKKTLALSVNKVYEMIKKRDFPFI
                     DEEIYALYNEFVEHFTKVKI"
     gene            complement(8024..9010)
                     /locus_tag="PLH17_03865"
     CDS             complement(8024..9010)
                     /locus_tag="PLH17_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20938.1"
                     /translation="MNEENKNPVEVEEENIPVEEKELKLEEIPLPEPEAIEYDTDNLK
                     NIEAAREAFLKQYKVQNIVKWIVSVAALALIIVGWLVFLDISVYLTIATVGASLLLIL
                     LYNFFIKRYLNGKMKIYFDEFYKNTTEFIFEGRDYSDVDFKVESKIEPVQFTENQIYK
                     DVIQVGSRNLTTYKYKGMPITVCDAAGQTKGAKQLTPVFVGKYFMAENSYDQDEPIIV
                     YLKGNEKSLPPTNVEHLNVISDDKQIAVYSNNAEALKFVNKKVMTALKKITTDDILID
                     VAIAIHKGKTFVCAGYDDVLMVLPLEKPFNPKATRSYRDDLVNLSEFIYTIK"
     gene            complement(9103..10056)
                     /locus_tag="PLH17_03870"
     CDS             complement(9103..10056)
                     /locus_tag="PLH17_03870"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HOR20939.1"
                     /translation="MNKSTNIIRYIAAGLGFLATLFFILPLYAGNGSVYIGAQIIFGF
                     QGANLDMFSFLSFLLPSAASIVLLIKTKHSESLATALFLLSGLVLILLPDFLVFFNDH
                     LGLSVILTTYVIPIVLSFMAALLGLTTAISKNSFSTYQIVEMAMLVGIAIVLDLPGLK
                     IRIGASGGSIGFTMVPLFILVLRQGPIKGFVGTGMVYGFATCILDGWGLVTYPFDYLL
                     GYGSLALLGIFQPFIVNEKATKFNIKGMLFLCLGVLIAIAGRLLASTLSGVIYYEVDF
                     WGSLAYNATYILPSAGIVLAVLIGLYDPIIRMDRMVKSRLS"
     regulatory      complement(10083..10188)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(10196..10717)
                     /locus_tag="PLH17_03875"
     CDS             complement(10196..10717)
                     /locus_tag="PLH17_03875"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOR20940.1"
                     /translation="MTKPEDIKVVIIGQDPYHEPNQAMGLSFSVPSSTRLPPSLVNIF
                     KEIESDLGVKMKNDGDLTYLAKQGVLLLNAYLTVRQSEALSHRLGEYDHFMDDVLDYI
                     NKLPQPIVFLLWGGFAKKYIKKITNPNHLILTSVHPSPLSANRGGWFGNRHFSKTNTF
                     LMSHNLSPIVWAN"
BASE COUNT         2838 a         2491 c         2470 g         2988 t
ORIGIN      
        1 cgaaacggca gtgcgaaacg accgcaatta tttatttcgc gtgtgttaaa atgaaaacat
       61 gagtttatta actaagcgtg agacgctcgt gcaaaacatc acctacatgg ccatcatggc
      121 ggcgatcaat gccgtctttt ccttgctcgc cgccctcgtc ccggccttat ccatcttctt
      181 aatgattgtt ctgcccctta gttcggcgat cgtcttttta ttttgcaaac acaaatactt
      241 tccgatctac gccgtcgcaa caattctcat ctgcttgctt gtcaccatct tcgatatgtc
      301 atttaccatc ttctatgtca taccatcaat tctgaccggc tatctttttg gcctgtttat
      361 caaatataag ctgcacgcgg tttggattat cctgattacg agcatcgccc agggattatt
      421 tacggccttg agcatcccct taatccaatg gatttttgat gtcaatatca tcgacacctt
      481 taaaggtttg ctccaagtca ccgcttccgt caatatcgat atcatcgtgc cgagtttctt
      541 attttatctg gcgttgacgc agatggtttt ttcctacatc gtcattcagt cggaaatcaa
      601 taaattcggc tacgaaataa gtgaccaacc aataaataca acgacgtatt cgctgattct
      661 tttcggcgtg atcatagcca tcataccgtt tatttttctt cttccgagcg gcgcttatct
      721 cctgttatca atcagcctct acttcatgtt ttttatcgtc ttcacgcata tcctgcaaaa
      781 acaatataaa gttctcatcg cctttggcat ttctctcctc gcttttatgt tcctttttgc
      841 ctttctttat ccgatcgttc ccacaccgtt tggccttctc ttggttagca ttcttccggc
      901 tcttatcgcc cttatttctt tgctgaacat tgtattgttt atgcgccgat caaaagataa
      961 aatgataagc acaggaaagg agacaaaaga atgattaaat tccttcagat ctttggtcag
     1021 ggcattctct acgtcgtcct ttctccgttt tttctcgtca tcctcgcctt ctatgtcgtc
     1081 tatacggcat tagtgatgat tttcatgttc ttaaagatga ttatcttgtt cttcgccggc
     1141 aagagcgtct tcgccgattt acccgaagac atcgaagccg aacagatgaa aaataaatta
     1201 cgagacgaga agctggccaa agaaaatcct cagacccctc cccttcctta tccgtatccg
     1261 ccttatggct atcccggtta tccttatccc ccgatgccgg gccaggtcat caatcaaccc
     1321 gatgtcaaag aaatcgacgc caaaccgatc gacgtcgaag aagaaaagaa ggacgagcaa
     1381 tgattccgtt tttaatcact atcagcgaaa acgaaaaacg gatgctcctt gttttactag
     1441 cggccgtcat cctcgttttt gttttaatcg gtctccttgg cctactcgtc aagcgcatca
     1501 tgtcttcgca agccaagcgc gcggatacat ttatgtatga tttggtctat accaaggcga
     1561 ttaaaaatcc ccggcacttt cgccgcgtgg gctttaaaaa gaaccaccgc ctcctctttc
     1621 agcaaagctg gattcctttc ttaatccttt tagtcgcggc agcttttctt gtgacctaca
     1681 tggccatcgc caatcactgg aatctcaatg tctttgatta ttacggcacg gacggaacgg
     1741 atggcgaagg ttttacgact ctattcttta tctttgattt tgcgaacgct ccgaaatcga
     1801 ctttcttcgg catgcaaatc gtctcggatt ggccgccgct tctctcctcg ccctatcttt
     1861 cagcaaatgc ctggccctca tatgtttttg ttcctatctt tctaacgggc gccatttggt
     1921 atctcatctg cgttcaggcc tttatcgccc gcacctggcg aattctcgtc atgtcgaaaa
     1981 agtcatttga aaagagcctc gataattacg atgcgaccaa aaatcccatc gataatccct
     2041 taccgcctct tcaataagtt atataaatat aaaggagtta cgaattaaag gcttgcatcg
     2101 caatcgatat catctcgtaa aggacgacgc cgacgagcag gatatcaatg atgataaata
     2161 agacaaaaaa gcggcggctg gaaagagctt tttgccgctt tttaatctcc gctagttctt
     2221 cttgggtctt ctcttgaata tcaaccatat tatattaata ttttatagca tttgccgaac
     2281 gggggtaagg ctgaacatcg cgaatattat tgataccggt aatatacatc aacaggcgat
     2341 caaagccgat gccgaagccg gcgtgggggc aaccgccgta cttgcggagg tcaaggtacc
     2401 actgcaaatc gggaagcaga tgcagcgctt ccatcttatc cttgaggatg tcgtagcggt
     2461 cttcgcgttg cgaaccgccg acaagctcgc cgacataagg cacgagcaag tcgcaggccg
     2521 cgaccgtttt gccgtcatcg ttttgataca tgtagaaagc tttgagatct ttaggataat
     2581 caattagaaa gaccggacca ttgacaacct tctcggtaat gtagcgttcg tgttcgctct
     2641 ggaggtccat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaccattgac
     2761 aaccttctcg gtaatgtagc gttcgtgttc gctctggagg tccatacccc agacgatatc
     2821 ggcattgtca aacttaacac cgtctttgac ggccttgagt aaaatatcaa tcgcctccgt
     2881 gtaggtcatc ttcttaaagt cggattccaa caaagtattt aagcgatcga gtaaggtatg
     2941 cgattgatcg aggatgttat taaaaaattc catctctaat gggcaacggt ctaaaacata
     3001 ttcaatgcag tatttaatgc aagcttcgat tagttccata tcatcgccta gatcagcgaa
     3061 ggcgatttca ggttcaatca tccagaactc gctggcgtgt cgcggcgtgt tcgaattttc
     3121 ggccctaaaa gtcggcccaa aagtatagac atcgcggaag gccatcgcga aagcttcgac
     3181 gtggagctgg ccgctgacgg ttaaggcgac atcttttccg aagaattcat tgctcttttt
     3241 cggaccggtc acgaccgaga aagtcgctcc cgcgccttcg gcgtcactgc cggtgataat
     3301 cggggtatga acatacatga agttctgttc ctggaagaag gtgtgaatcg ccatcgttaa
     3361 gacattgcga acgcgaaaga cggcgctgaa ggtgttggtg cgcgccctga ggtgggcgat
     3421 atcgcgtaaa aactcgaccg agtgatattt cttttgcaag ggaaaatcat ccgcgcattc
     3481 gccgaccaaa tcgagttctt tgacgtgaat ttcaaagggc tgtttggctt cgggcgtcaa
     3541 aacaaaatcg cccatcacgc gaattgagga acctgtttta atttttgaga tggaatcgta
     3601 attatttaac ttatctcggg agtagaccaa ctgaatgcct ttaaaattcg tgccgtcgtt
     3661 aaaatcgata aagccgaccg ccttgttatc acggttagat ttaacccagc cctcaaggta
     3721 aacgccctgt ttgagcttat ctttaaattc ttcatcatcg ccataggtga tgacataaag
     3781 gtcatagacg gtaatgctta aaggttccat atttttactc cgctgctcgt tttttaatta
     3841 taacaattaa caccgcatat atcacacaca aatcagaatt tgtcgtaatc ggaaatccgc
     3901 cagttgggat cgacgccgat atccatatta gcgaatatct tacgctcata gagatggtcg
     3961 gctaatttgg cgatcatcgc cgcattatcg gtcgtcgccc ataagggcgg gatgatgaca
     4021 ttgatatccg tgccggcaaa atagttgccg atttgttcgc gcagatacga gttcgcagac
     4081 acccctccgg ccaagacgac ttgctttacc tgatagtgct tagcggcggc aatcgtctta
     4141 tcgacgacca tgccgatggc gatatcctga aacgacttgc acatatcaaa aatgttgatt
     4201 tcttcgtctt tttgattgag cgaattgacg agattgacga ccgcggtctt aagaccggaa
     4261 taggagaagt caaagcctcc gttgcgcaaa ggcgtcggga gtttataagt cggtttccct
     4321 aatttggcgt tccgatcaat cggcaggcca ccaggataag gaagaccgag gacgcgggcg
     4381 actttatcaa agcattctcc gacagcatca tctttggttt ctccgacaac ctcaaactcg
     4441 agatgccggg gcatataaac taattccgtg ttgccgcccg atacgaccgt gcaaagcatc
     4501 ggaaactgaa agggtttaat gtattcattc gcgtagatat gggccgctaa gtgatggacg
     4561 ggaatcaggg gcttttgata tatcaaagcc aaggtcttgg ccatctgcat gccgacgtgt
     4621 aaagcgccga tcagaccggg gccgcgggta aaagcgaaag catcgacttc ttcaagttta
     4681 acatttgctt tttcgagcgc ttcatttaag acgatcgtga tgttttcggc gtgaaggcgc
     4741 gccgccatct cgggcatgac gccaccgtat tttttatgca tctcaatctg ggtggcgacg
     4801 acattgctta acaaagtccc atcacgagtg atggcgactg ccgtttcgtc gcaacttgat
     4861 tcgatggcga gaattaaagc catactagat taatctcctg accatataca aagcatcctc
     4921 gccgtttgaa taataggcgg gcttgattaa gacgccttca aagccgtgct tttcataaaa
     4981 ggcgatggcg gcttgattat gggttcttac ttctaaggta atcgtctcga tgatttcgtt
     5041 atattcattt tttaaatgct caatcatctt gttaagaagc tctgttccca gatgctgctt
     5101 gcgatatttt ttagcgatgg cgatctgcga gatggtgctc gaagaaaagg tgaccataaa
     5161 atcgatgaag ccggcgattt ctttgacgcc gttatcaatc acttccaaga caaagatgac
     5221 gttgatcggg ttttccgtca attcgtaaag ccagtcgcgc cgggtccagg gcgtgacaaa
     5281 agattcttct tcgatttcaa gaacgcggtc taaatccgcg ttttccatgc ggcgaatagc
     5341 agttacaagg ctcatagcaa atcctttaga tagaccggct tcaaagttaa gatattttct
     5401 accggaatac tcttcttttt cgcttccagc attccgtcaa gaacgctggg ctgataagca
     5461 tcgataccaa gatatttggc gtctcccgaa acgatgaaat cggcattgcc cttaatccag
     5521 gcaatcagtt catcattggg ccatactttt tctatgacca agacttggcc atcggcgtca
     5581 tagatggcga catagctgcg attgctgcgg gcattaatta ctgaaattgt cggttgatgc
     5641 ggggcttttt gcgaaataag cgaggagacg gcatagattt tgatatcgag ggcgaacgct
     5701 aaagttttag cgatggtcaa ggcaatccga atgccggtat aggagccggg gccgatcgtc
     5761 accaccactt cgccgatgtc tttagcggat atattattgc gctttaaaat ggcatcgact
     5821 tcgacggcca tcagctcgct ttgccgctgc caggcctgat aggagatgcg gtcgatgatc
     5881 tgattatctt tcgccaatcc gacgtttaaa tcgcggttcg cgctgtcgag gaggaggcta
     5941 tacattttcc gtctccttta agcttttgat gacttgttca taggaggagc caatattttc
     6001 aaaagtgatg cgacgcgagt tttcgccaag cgcttcaatg acgatgcgaa gatggcttgc
     6061 cggaattaga ggtttaatgt aatcaggcca ttcaattaag gtgacgcctt cgccttcgat
     6121 aaattcttca agcccgagtt cgctgtcgcg attttctaag cggtaggcat cgatgtggaa
     6181 catcggaagc cgtgcttcga aataacattt cattatatta aaagtcggcg agatgacgcg
     6241 ttcgaggata tcaagtcctt tggcgacgcc gccgacaaaa gtcgtcttgc cgcttccgag
     6301 cggtccatct aaagtaatga ccgccccggg aaataaaaag cccgccagcc gcccagcgac
     6361 gcgcttggta tcttcacttg attttgaaat aaatatgtat ttcaagtctt ggtccaactt
     6421 tcgcctgcca ttatagcaaa taaccgaatg tgtagtataa tacttcgtag atttatgtta
     6481 aaagtcatcg tcatcggagc gggcccgatc ggcctctacc tcgccgcaag ccttaaaaag
     6541 gcgaatattt cacatctcgt cctggaagcc ggagaaaagg cgggtggtca gcttaccgct
     6601 ctttatccag aaaaggaaat cgttgatctg cccggcattc caagtattat cgccaaagat
     6661 tatattgctc tcctagccaa agatgtcgat ttgaataatt tccgttttcg tcaggaagtc
     6721 atcgacatca aaagctttga agaccacgtc gaagtcatcg ctaaagaaga ccattatcaa
     6781 gctgaatata tcatcatcgc gaccggactt ggtttttata aaccaaagcc aatgggttta
     6841 gctcgcgaaa acgagtttgc caatatttta tattccttat cgagttttga tttcctaaag
     6901 ggaaagagag ttgccatctt cggaggcggg gattccgccc tcgactgggc caaggaaata
     6961 tcgcgggtta ccaatagcat ccatctaatc caccgccgcc gcgaattccg cggtaatttt
     7021 gaaacaatca aagatatcaa agaaataaaa attcacactc cttatgtgcc aagccagtta
     7081 atcattgaag ataaccacct ccttggcgtc agaatcaaac atgccgaaga agaatcgtat
     7141 gtcgacttat ttgtcgacta tatcctcgtc aattttggca atgtccccag ccctgtcgcc
     7201 ttcagcgttc ccaaacaaaa taccggcata atctgcgatg agtatcgctg cgtcttaaac
     7261 cgcgtctatg tcgcgggtga tgcgattggg gtaccgggca aaataaaaag aatcggtgat
     7321 ggtcttaaag atgccgattc cgtattaaaa acgattctta aattttgact ttggtaaagt
     7381 gctcgacaaa ctcgttatat aaagcgtaga tctcctcgtc gataaacggg aaatcgcgct
     7441 tttttatcat ctcgtagact ttgttgaccg agagggccaa agtcttttta atatcttcgc
     7501 tgtaatcaag cgaataggca tggcgttcgt actcgttttg atctaaaagt tttgtggagc
     7561 cgcccggaaa gagtttgatg tctaagtcat agtcgatata tttgatgcaa ttggcgtgaa
     7621 tcaccgccgg agaggcgatg tttacatagt aagagatgtc ctgaccttcg tctttgagca
     7681 tggcgataac gttcatccat tcctgtttga aaaagatgaa gacggccggc tcgcgggtgt
     7741 gccatttcct gccgtcgtgc tcgacgacgc tggagcgcac cgaaattaaa atgatgtgcc
     7801 ggtcgttatc ttccaagaca aagccatggc tccaggcgcg atgcagttcc ccgttatgtt
     7861 tataagcttg gattctaagc cagcgtcctt cgtaattcat tgttatttcc tattaaggaa
     7921 tcaaaacccc cggttccgat atcggtttta ttataacatc aaagttttag gagtgttaaa
     7981 aaagcctccg gggagaggct ttgttatgct gtgctgattc tttttatttg atggtgtaaa
     8041 taaactcgga gagattgact aaatcatcgc ggtaactgcg cgtcgctttg ggattaaatg
     8101 gcttttctaa tggtaaaacc attaagacat catcatagcc ggcgcagaca aaggtttttc
     8161 ctttgtggat ggcgatggcg acatcgatta agatgtcatc cgtcgtaatc ttttttaagg
     8221 ccgtcataac ttttttattg acgaacttca aagcctccgc gttattggaa tagacggcga
     8281 tttgtttgtc gtcgctgatg acattaaggt gctcgacatt ggtcggcggg agcgactttt
     8341 cgttgccttt gagatagacg atgatcggct catcttggtc atagctgttc tcggccataa
     8401 aatacttgcc gacaaaaacg ggcgtcagct gcttcgcgcc tttggtctga ccggcggcgt
     8461 cgcacaccgt aatcggcatg cctttgtatt tataagtcgt caggttgcgg cttccgacct
     8521 gaatgacatc tttgtagatt tgattctcgg taaactggac gggttcaatc ttgctttcaa
     8581 ctttgaagtc gacatccgaa taatcccttc cctcgaagat gaattccgtc gtattcttat
     8641 agaactcatc gaaatagatt ttcatcttgc catttaaata acgcttgata aagaagttat
     8701 aaagaaggat gaggaggagc gaggctccga cggtcgcgat ggtcagataa acgctgatat
     8761 ccaaaaagac gagccagccg acgatgatga gggctaaagc cgcgaccgaa acgatccatt
     8821 tgacgatgtt ctgaacttta tattgcttta aaaaggcttc gcgggcagct tcgatatttt
     8881 tcaggttatc ggtgtcgtat tcaatcgctt ccggctcagg aagaggaatt tcctcgagtt
     8941 taagttcctt ttcttcgacc gggatatttt cttcttcgac ttcgactgga tttttgtttt
     9001 cttcgttcat aagcttctcc acgttaggtc aaacataaag tttgcagcga ctacaatata
     9061 tcataagcgc cgccagttac gaaaagaaag tgtttatcat tattaagata aacggctttt
     9121 aaccatccgg tccatgcgaa taatcggatc ataaagaccg atcaggacgg cgaggacgat
     9181 gccggcgctg ggcaggatat aagtagcgtt atacgctaat gagccccaaa aatcgacttc
     9241 gtaatagatg acgcccgaaa gcgtcgaggc caagaggcgc ccggcgatgg cgatgagcac
     9301 gccgaggcat aaaaagagca tacccttaat attgaacttc gtggcttttt cgttgacgat
     9361 aaagggctga aaaataccta ataacgccaa tgagccatag cccagtaggt agtcaaaagg
     9421 ataggtgaca agaccccagc catcgagaat gcatgtcgca aagccgtaca ccatgccggt
     9481 gccgacgaaa cctttgatcg gcccttgtct taaaaccagg ataaaaagcg ggaccatcgt
     9541 aaagccgatc gaaccgcctg aagcgccgat gcgaatctta agtcccggca agtctaagac
     9601 gatggcgatt cccaccaaca tcgccatctc gacgatctga tatgtcgaaa aggaattttt
     9661 gctgatcgcc gtcgtcagac cgagcaaggc ggccataaac gacaggacga tcggaatgac
     9721 atatgtcgtc agaatcacgc ttaagcctaa gtggtcatta aagaaaacaa gaaaatcggg
     9781 aaggaggatg aggacgaggc cagagagcaa aaaaagggcc gtcgccagcg attcggaatg
     9841 ctttgtttta atcagcaaaa cgatcgaagc ggcagatgga agcagaaacg aaagaaaact
     9901 aaacatatcc aggttggccc cttgaaagcc aaaaattatt tgcgcgccga tataaacgct
     9961 tccgtttcca gcatagagcg gaaggatgaa aaatagcgtc gccagaaagc cgagcccagc
    10021 ggcgatataa cggatgatat tagttgattt gttcataaaa aattcctcgg ccggcgagga
    10081 agacaaagat aaatctttat ttcctccgct agcattatct agatcaggtc aggtcttctc
    10141 cggtcgcaag aactctcagc ccacgcgtga gctcccttta gatagtatag cctttttagt
    10201 tagcccagac aatcggcgaa agattgtggc tcattaagaa ggtattggtt ttggaaaaat
    10261 ggcggttgcc gaaccatccg ccgcgattcg cgcttaaggg cgacgggtgg acggaagtca
    10321 aaatgaggtg gttgggattt gtaatctttt taatgtactt tttggcaaag ccgccccaca
    10381 gcaagaagac gattggttga ggaagtttgt ttatatagtc aaggacatca tccataaaat
    10441 ggtcgtactc gccaagacga tgcgaaagag cctcgctttg gcgaacggtc aagtaagcat
    10501 tgagaaggag gacaccttgc ttggcgagat aagttaagtc gccgtcattt ttcatcttga
    10561 cgcctaagtc actttcgatt tctttaaaaa tattgacaag gctcggcgga agccttgtcg
    10621 aagacggaac ggaaaaggaa agacccatcg cctgattggg ctcgtgatag gggtcctggc
    10681 cgatgatgac aaccttgata tcttctggct tcgtcagatt aaaggcgttg aagacgaggg
    10741 ggcggggggg gatagacgat atgttttgaa tattcctcgt cgagaaaagt ttttagactt
    10801 ttcgcatagt tctttgaagc gatttcttta aaaaataagt cccagctcat ggagtgtttg
    10861 tttcctgata ttttattata taagcat
//
LOCUS       DAOSSL010000033         9789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_6699_length_9789_cov_15.648551, whole genome shotgun sequence.
ACCESSION   DAOSSL010000033 DAOSSL010000000
VERSION     DAOSSL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6699_length_9789_cov_15.648551"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..842
                     /locus_tag="PLH17_03880"
     CDS             105..842
                     /locus_tag="PLH17_03880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOR20941.1"
                     /translation="MIFAQALDFSSLLNEEIVALIIYPLLYLTYYFASDFPIIKKFRN
                     RFGETKDNFEQSVYFRRSLGFVLLGLIPFFVTLFYFQNPLGDYGLGLPSGEYALWWSL
                     VPLAAVLVVSFALPGNNIDLSYYPEVRKPEWTLKRTAINAAFWAIYLLGYEFALRGML
                     FYTTFYAYGLWPSIAINSAIYALIHIFKGPKEAFGAFFLGILLCLITYYTNSIWAAFI
                     IHVVMAVANDIKAVNAAKKVNNSAKSQ"
     gene            complement(866..1339)
                     /locus_tag="PLH17_03885"
     CDS             complement(866..1339)
                     /locus_tag="PLH17_03885"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOR20942.1"
                     /translation="MDIYNFTMKDITGKDVSLADFKNKVLLIVNTATGCGFTPQYDGL
                     EALYKKYRDKGFEILDFPCNQFKNQAPETNAEIASICKLRFDVTFRQFSKIEVNGNNE
                     APLYTYLKSQKSTPLGKNIKWNFTKFLVNRDGTVIDRFAPAVKPEEIEKYIVSLL"
     gene            1523..>2391
                     /locus_tag="PLH17_03890"
     CDS             1523..>2391
                     /locus_tag="PLH17_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20943.1"
                     /translation="MKLRLAKINFRDGIANIFPYLILLILSFLALFVFFYEGLLQGDD
                     IVFHLANISDEYLSLINQGSISRISTYLGSNLGIGTRLFYSPLFHLLASLLYASTQWL
                     GADAVTAIKIVMFVSVFVSGLFMYRFLLKVTNGKTVASIIGAAIYVLYPYRIFDALCR
                     AAYAEALAFVFIPLFFKGLYGLVNFKEKITVLPFVEVILGGSLLFLSHNLTAVFGFIF
                     GVIFLLANTHKIIILCRQPRYWITGLLSIVFLVGIMSVTLFSSLELMSSGLYNISNPG
                     RMWTTISAVTSRI"
     assembly_gap    2392..2491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2492..3295
                     /locus_tag="PLH17_03895"
     CDS             <2492..3295
                     /locus_tag="PLH17_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20944.1"
                     /translation="ALITQKWLWRIMPEPLLMIQFPWRLFAFIQFFASWAIAWLCYKF
                     EFKKAVPYAAVTALGFCLVASQALPEKRLNMLRASEPESSMVVYHGDEDYFAPTSSIG
                     WNKEYIPQVFFQNGYVSAYSNTLYYSIKQRINQPYLETYPLNPVALSGDCAIDVVERE
                     TPNYELGVTPLEDSLVQMPLIYYPGYRIDAYDADGTKAKLEAQNIDGLIAFELAVSHQ
                     RITISFAGSPLVIASYVYFGLSVIGTAGLVVWFKCFDRTKKEKQNDVIH"
     gene            3393..4595
                     /locus_tag="PLH17_03900"
     CDS             3393..4595
                     /locus_tag="PLH17_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOR20945.1"
                     /translation="MHNARKIDDGLYYVGVNDHRITRFENTHPLTKGMAYNSFLLIDD
                     KTVLLDTVDRSGGQQFMENVAAALAGRKLDYLVINHMEPDHCALIVDIFRTYPEVILV
                     GNKTTFRLLDQFYGVDLRGQFVEVKEGDTLSIGKGTLRFFMAPMVHWPEVMVTYEEKS
                     GLLFSADAFGTFGTIEGSLFTDAEHFEKLWLDEGRRYYTNIIGKYGMQTLSLLNKIAQ
                     LKINMVLPLHGPLWRDDIASYFKKYRLWASYAPEEKGVVIAVGSIYGNTEEAADILAG
                     YLADLGVKNIVMHNVSSVDPSFILSDIFRYSHVVLISATYNFRVFPKMETLVTDIVAT
                     GVANRTFAIIENGSWAPAADNLIRAQISKLKNARIINQEKFTIKSALKSNQLEALKTL
                     ARQIAETI"
     gene            4643..4993
                     /locus_tag="PLH17_03905"
     CDS             4643..4993
                     /locus_tag="PLH17_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HOR20946.1"
                     /translation="MKDFLITTTHQLEGYIITQYLDIVFEESIFGISISTGIKGISDM
                     FAGWTGERLHAISDRIEEVKNEIKEALINKAVRLGADALIGVDVETTRNLNDGTIGVS
                     MSGTAVRVVKAIAK"
     gene            complement(5056..5997)
                     /locus_tag="PLH17_03910"
     CDS             complement(5056..5997)
                     /locus_tag="PLH17_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOR20947.1"
                     /translation="MPLVTSKELFAKAYQGKYAIGAFNVDNIDIMKAVLKAAEQTKSP
                     VIIAISSGALDFIGKDFIRNAMHDAMKKITVPVVLHLDHGKSAALCKECVDIGFTSVM
                     IDASAYEFDKNIEITKEVVEYAHARGVVVESELGAIAGIEEDIHVEDKYGHFTHPADV
                     VEFVSKTGIDSLAIAIGTAHGAFKFKPGQKPQLRFDILEEIQAKLPGFPLVLHGASSV
                     PKAYLDTLNRYGGKMPEAIGIPEDMLRDAARRAVCKINVGTDIRVAYVGGIRKALQEN
                     PQEFDTRLFINAGLKAITDLVADKMVNVLGSAGHAND"
     gene            complement(6040..6975)
                     /locus_tag="PLH17_03915"
     CDS             complement(6040..6975)
                     /locus_tag="PLH17_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOR20948.1"
                     /translation="MPLVNTKEMFQKAYREGYAVGCFNVDSIAMVQAVLEAAEENRSP
                     VMVAFSKGSREFMHPGNIMILIKIVAKDISIPFAIHLDHGKSAELCKACIDEGFTSVM
                     IDASMHPLEENIKITKEVCEYAHARGVSVEGELGAIAGDEDHPKQETRKNTFTNVDDV
                     VRFVEETGVDSLAVSIGTVHGMQKFAPGEIPSLRFDILEEIQRRLPEFPLVLHGASSV
                     PPEILEKFNRYGGNIHDAIGVPEELLVRAASLNVCKINIGTDFRMAYVGGLRKALADY
                     PDRFEPRYFLAPAKEAARNLVAHKLANVFKSANRV"
     gene            7150..8433
                     /locus_tag="PLH17_03920"
     CDS             7150..8433
                     /locus_tag="PLH17_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20949.1"
                     /translation="MKKAFVFLCASLFVTSAVVLTGCNPTTDDGSTAAVVEKASKMTL
                     AELEAASKAEMEASDSTFKVVGLTSTLAKAMVKFCETYDWMELDVNTYVNNSYKDYAL
                     LTALEQADTSYFADFALVQDARSLADYLEAGITVNYVPSDYEELNLPEENTYPLKGLY
                     FNKIFFSNERLGVDLNNIWQLAGSPEDANHLDNVSFQSPITEQINMSFILSLYDEANQ
                     ERLETAYENYYGTAWVASEDYESCGDQFVNEFVANISRWHSSDGTAMKKTQYEETAQE
                     GVDPFVYYGAFAKMKDAAGAAYGEETAMVTVDWDKEIDGFNGFMYAMYSQIVKNAEHP
                     YTACLYARFLLTPESYEAMCYNSSTPNSLGEPANMYGYYYPCTSTTVGINDNDWSKEE
                     WIEKSINEDFNYLSTVKGARINEILALVSSNTKAS"
     gene            8533..>9789
                     /locus_tag="PLH17_03925"
     CDS             8533..>9789
                     /locus_tag="PLH17_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOR20950.1"
                     /translation="MKTMSFSKIWNKTKTFFSKPANIILLFFTILLTATVIYPLISLV
                     LDSFTVQSISEAREINEIWGTAVKKGDFTFAQWPNLLFNANSEYSLYFFWQPLYKSVL
                     MALLACVIAVLGGGVLAWLITRSNLPMKKYISAVFIFPYIMPSWSIAMFWENFFKNTN
                     IAASNGTGILQALTGICVPEFLVYGIVPCALVLGIHYAPFAYILIGGILRNMDANLEE
                     AATILKSSRFKILRRITLPIVAPALISTILLVFASSISSYTVPAFLNANNSFTSISIA
                     MRGLIITAGKKGQGYVVAVILLAFSVLILTINNWFTRSRRSFTTVSGKSGQVSKFRIG
                     NKPWIKWVVASIIVLLVSFFAVFPLVSFVLESLQETSGNYASMTFKYWFSPENFDVRI
                     TAQGQSSQGIFHNYTIWRAFGRSVLVA"
BASE COUNT         2502 a         2320 c         2092 g         2775 t
ORIGIN      
        1 accgcggcgg gaaagattgc caatttaatg tatcggatat tccccagatt gtctggttac
       61 ctcataagaa aatacgctat gaagaataaa ggatacaaat caaaatgatt ttcgctcaag
      121 ctctcgactt ttcatccttg ctaaacgaag agatagttgc tttgattatt tatccgttgc
      181 tctacctgac ctattatttt gccagtgact ttccaataat taagaaattc agaaaccgat
      241 ttggcgaaac aaaagacaat tttgaacaat cggtctattt tcgccgctcg ttagggtttg
      301 ttcttcttgg tctgattcca ttttttgtca ccctctttta ttttcaaaat ccgcttggcg
      361 attatggact cggtcttccg tcgggcgaat acgccctttg gtggtcatta gttccgttag
      421 cggccgtgct cgtcgtcagt ttcgcccttc caggtaataa catcgatctt tcttattatc
      481 ctgaggttcg caagcccgag tggacgttaa aaaggacagc catcaatgcg gctttttggg
      541 ctatctactt actgggatac gagttcgctt tgcgtggcat gttattttac acgacgtttt
      601 atgcttatgg tttatggcca tcaattgcca tcaacagcgc gatctatgcc ctcatccaca
      661 tttttaaagg tccgaaagaa gcttttggag ctttcttttt aggaattctt ttgtgcttga
      721 taacatatta tacgaactcg atttgggccg cttttattat ccatgttgtg atggcggtcg
      781 ctaatgatat taaggccgta aatgctgcta aaaaagtcaa taattcagca aaaagtcaat
      841 aattcagtaa tttttaatat actttttata atagactaac aatgtatttt tcgatctctt
      901 ctggttttac ggccggagcg aatcgatcga taaccgtccc atcgcgattg acgagaaact
      961 tcgtaaaatt ccatttgatg tttttgccaa gcggcgtgct tttttggctc ttgagataag
     1021 tataaagggg cgcttcattg ttgccgttga cttcgatttt ggaaaactgt ctgaaagtaa
     1081 catcaaagcg gagtttgcag atactagcga tttcggcatt agtttccgga gcctgatttt
     1141 taaattgatt gcacgggaag tccaagattt caaatccctt gtcacggtat tttttataga
     1201 gggcttccaa accatcgtac tggggtgtaa aaccgcatcc ggtcgcagta ttgacaatta
     1261 atagaacctt gtttttaaaa tccgccagcg aaacatcttt accggtgatg tctttcatcg
     1321 tgaaattgta aatatccata tttatatcct cgccttcatc ttcaatgatt cgagcccgga
     1381 tgacaacgaa tatgctactg aggcatactc tttttaacta tccgaaggtt gcgctttgtt
     1441 ccgcagatga cattttctat tttcttcgtt tttaaatagg cgttgcctga cgaatgtatt
     1501 atattaaata ataagaagca tcatgaaatt gagattagca aaaattaatt tccgggatgg
     1561 catagccaat atttttcctt acctcatcct tttaattctt tctttccttg ccttattcgt
     1621 tttcttttat gagggtcttt tgcaggggga cgacatcgtt tttcatctcg ccaatatcag
     1681 tgatgaatat ttgtcgttaa tcaatcaggg ttcgatttcg cgcatctcga cttacctcgg
     1741 ttcaaatctc ggcatcggaa cgcgcctttt ctactcgccg ctttttcatc ttctagcgag
     1801 cttattatac gcatcgactc agtggctggg cgcagacgcc gtaacggcta ttaaaatcgt
     1861 gatgtttgtc agcgtttttg tctccggttt gttcatgtat cgcttccttt taaaagtcac
     1921 taacggaaag actgtagcct cgataatcgg tgcggcgatc tatgtcttat atccctatcg
     1981 catctttgat gcgttatgcc gggcggccta tgccgaagcc ttggcgtttg tctttattcc
     2041 cctctttttt aaggggctat atggcttagt caatttcaag gaaaaaatca cggttctgcc
     2101 ttttgtcgaa gtcatcctcg gcggatcgct gctttttctg agccataatt taaccgccgt
     2161 ttttggtttt atctttggcg tcatttttct attagccaat acccataaaa tcatcatctt
     2221 gtgccgtcag cctcgctact ggatcaccgg tttgctctcg attgtttttc tggtcggaat
     2281 catgtccgta accctcttct catctttaga attgatgagc agtggccttt acaatatctc
     2341 caatcccggc cggatgtgga cgactatcag cgccgtcacc agccgcatcg annnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccctaatc acccaaaaat ggttgtggcg
     2521 catcatgcct gaaccgcttc tgatgattca attcccgtgg cgccttttcg cctttattca
     2581 attctttgcc agctgggcaa tcgcctggct ctgctataaa ttcgaattta agaaagccgt
     2641 gccttatgcg gcggtgaccg ccctcgggtt ttgtctggtg gcctctcaag ccttgcccga
     2701 aaagcgcctc aatatgctgc gggccagcga accggaaagc tcgatggtcg tctatcatgg
     2761 cgatgaagac tatttcgcgc cgacatcttc aatcggctgg aataaagaat acatccctca
     2821 ggtctttttc caaaacggtt atgtttctgc atatagtaat actttgtatt atagtatcaa
     2881 gcaacgcatc aaccagccct acctcgagac atatcccctg aatccggttg cgctgagtgg
     2941 cgattgtgct atagatgtcg tcgagcgcga aacgccgaat tacgaattgg gcgtcacgcc
     3001 cttagaagat agcctcgttc agatgccgtt aatttactac cccggatatc gcatcgacgc
     3061 ttacgatgcg gacggcacaa aagccaaact cgaagcccaa aatatcgatg ggttgatcgc
     3121 ctttgagcta gccgtcagcc atcaacgaat taccatatcc tttgctggaa gcccgctggt
     3181 catagcatca tatgtctatt tcggcttatc ggtcattggc acggccggat tagttgtctg
     3241 gtttaaatgt tttgaccgaa caaaaaaaga aaagcaaaat gatgtcattc attgatggct
     3301 ttttctttgg ggcagcctgt ttttcgtcca taacaattgt taattagcag ctcagtcagt
     3361 ataatttttt tgtcgagata aaggagattt atatgcataa tgcaagaaaa atagatgatg
     3421 gtttatatta tgtcggcgtc aacgaccatc gcatcaccag atttgaaaac acccatccgc
     3481 tcaccaaggg gatggcatat aattcttttt tactaatcga tgacaaaacg gtcttactcg
     3541 acaccgtcga ccgctcgggc ggccagcaat tcatggaaaa tgtcgcagcc gctttagcgg
     3601 gtcgcaaact cgattatctc gtcatcaatc acatggaacc cgaccactgc gccttaatcg
     3661 tcgatatttt cagaacttat ccggaagtga ttttagtcgg aaacaagacg acttttcgcc
     3721 tccttgatca attctatggt gtggatttac gcggacaatt cgtcgaagtc aaagaaggcg
     3781 atactctcag catcggcaag ggaacgctca ggttttttat ggctccgatg gtccattggc
     3841 cggaggtgat ggtcacctat gaagaaaagt cagggcttct tttctccgcg gatgcctttg
     3901 gaacattcgg cacgatcgag ggaagcctat tcaccgatgc cgagcatttt gagaaattat
     3961 ggttagatga aggccgccgc tactatacaa atatcatcgg aaaatacgga atgcagacgc
     4021 tatctttatt aaataaaatc gcacaactga aaataaatat ggttctccct cttcacggcc
     4081 ctttatggcg cgatgacatc gcctcgtatt ttaaaaaata ccgcctgtgg gcatcatatg
     4141 cccccgaaga aaagggcgtc gtcatcgccg tcggttccat ttatggaaac acagaggaag
     4201 ccgctgatat tttggctggt tatctcgctg acttgggtgt caagaacatc gtcatgcaca
     4261 atgtctcatc ggttgacccc tcgtttatcc tcagcgatat ctttcgctac tcgcacgtcg
     4321 ttttaatatc ggcgacatac aatttccgcg tctttccgaa aatggagacg ctcgtcaccg
     4381 acatagttgc caccggagtt gctaaccgca cttttgccat catcgaaaac ggctcgtggg
     4441 caccagccgc cgacaacctc attcgcgccc aaatcagcaa actgaaaaat gcgcgcatta
     4501 ttaatcagga gaaattcacg attaaatccg cgcttaaaag caatcaactt gaagctctta
     4561 aaactttggc tcggcaaatc gccgaaacga tttgattatt tgttagtgtg ttagtcgcaa
     4621 atattttaaa ggggatagaa atatgaaaga ctttctgatt acgacgacac atcaattgga
     4681 aggatatatc atcacccaat atcttgatat cgtttttgaa gagagtattt ttggcatcag
     4741 catttcaacg ggcatcaaag gcataagcga tatgttcgcc ggttggacgg gcgaaagact
     4801 tcacgccatc tccgaccgca ttgaagaagt caaaaacgaa atcaaagaag cgctgattaa
     4861 caaggctgtc cggctcgggg ccgatgcctt gatcggcgtc gatgttgaaa cgacgagaaa
     4921 tctcaacgac gggacaatcg gagtgtcaat gtcgggcacc gcggttcgcg tcgtcaaagc
     4981 catcgcaaaa taaaaacata caaaaagcgc ggtaattgtt gcaaatccgc gcttttttat
     5041 tgctatttcg aataattagt cgttggcgtg tccggccgaa cccaagacat ttaccatctt
     5101 gtccgccacc aaatcagtga tggctttaag gccggcgtta ataaataagc gtgtatcgaa
     5161 ttcctgaggg ttttcctgaa gcgccttgcg aatgccgccg acataagcga cgcggatatc
     5221 ggtcccgacg tttattttgc aaacagcccg tctggccgca tcgcggagca tgtcttcggg
     5281 aataccgatg gcttccggca tcttgcctcc atatcgatta agcgtatcaa gataagcctt
     5341 tggcacgctc gaggctccgt ggaggacgag tggaaagccc gggagcttgg cttggatttc
     5401 ttcaaggatg tcaaaacgaa gctgtggttt ttgccctggt ttgaatttaa aggctccgtg
     5461 ggcggtgccg atggcgatcg ccaagctgtc gattccggtc ttgctgacaa attcgacaac
     5521 atcggcggga tgggtgaaat gcccgtattt atcttcgaca tggatatctt cttcgatgcc
     5581 ggcgatggcg cctaattcgc tttcgacgac aactccgcga gcgtgagcgt attcgacgac
     5641 ttctttcgtt atttcaatat tcttatcaaa ttcataagcg gaagcatcga tcatcacgct
     5701 ggtaaagccg atatcgacac actctttgca taaagccgcg cttttgccgt ggtcgagatg
     5761 tagtacgacg ggaacggtaa tcttcttcat ggcatcgtgc atcgcatttc gaatgaaatc
     5821 cttgccgatg aaatcaaggg ctccgcttga gatggcgatg ataaccggtg acttggtttg
     5881 ttcggcagcc ttcaatacag ccttcataat atcgatgttg tcgacattaa aggctccgat
     5941 ggcatacttt ccttgatagg ctttggcaaa cagctctttt gacgtaacta atggcataat
     6001 ctttcctcct tatgcttatt gcataactat taaattaatc tatacacgat tggcactttt
     6061 aaaaacatta gccaatttgt gcgcaacgag attgcgggcg gcctctttcg ccggtgctaa
     6121 gaaatagcga ggctcaaaac gatcgggata gtcggcaaga gcttttctta gccccccgac
     6181 ataagccatg cggaagtcgg ttccgatatt tattttgcag acattgagcg aagcggctct
     6241 tacaagcagc tcttctggaa cgccgatggc gtcgtgaata ttgccgccat agcgattgaa
     6301 tttctcgagt atttcgggcg ggacgctgct cgcgccatga aggacgagtg ggaattccgg
     6361 caaacgcctt tgaatttctt caagaatgtc aaatctaagt gagggtattt ctccgggcgc
     6421 gaatttctgc atgccgtgta cggttccaat cgagacagcg aggctatcga cgcccgtttc
     6481 ttcgacaaag cggacgacat cgtcaacatt ggtaaaagta tttttcctcg tctcctgctt
     6541 gggatggtct tcatctccgg caatcgctcc caattctccc tcgacgctga cacctctagc
     6601 gtgtgcgtat tcgcacactt ccttagtaat tttgatattt tcttcaagcg ggtgcatgga
     6661 ggcatcgatc atcacgctcg tgaaaccctc atcgatgcac gctttgcaca gttccgcgct
     6721 tttgccgtga tcaagatgaa tagcaaaagg aatcgaaata tccttggcga cgatttttat
     6781 taatatcatt atattgcccg ggtgcatgaa ctcgcgcgag cctttagaaa aagctaccat
     6841 aaccggagaa cgattctcct cggcagcctc gagaacagcc tgaaccatcg cgatgctatc
     6901 gacattaaag cagccgacag cgtatccttc ccggtacgct ttttgaaaca tttctttggt
     6961 gttgactaat ggcatgctga ctcccgtgac aacccgacaa atcgggtccc taatataacg
     7021 atatcactaa aaataataga actaaatgaa aaaaatgtat atttgtaaac gattacaata
     7081 aaaatattgt ttataaaaaa atcccgtgct aaaatatttt cagaaagatt ctaggaggat
     7141 cacaaaaaca tgaagaaagc atttgtattt ctatgcgctt cacttttcgt cacgtctgcc
     7201 gtcgttttga caggctgcaa tccgactact gatgacggtt caaccgccgc ggtcgtggaa
     7261 aaagccagca aaatgacgct cgcagaattg gaagctgcct caaaagcaga gatggaagct
     7321 tctgactcaa cattcaaagt cgtcggtctc acctcaaccc ttgctaaagc aatggttaag
     7381 ttctgcgaaa cctatgactg gatggaactc gacgtcaaca cctacgtcaa taacagctat
     7441 aaggattatg ccttattgac tgctcttgaa caggctgaca cgtcatattt tgccgacttt
     7501 gccctcgtcc aggatgctcg cagcttggcc gattatctcg aagccggaat taccgtgaac
     7561 tatgttccaa gcgactacga agagctcaat ttgccagagg aaaacaccta tccgttaaaa
     7621 ggcctttatt tcaacaagat tttcttctcc aatgaaagac tcggcgtcga tctcaacaac
     7681 atttggcaat tagcgggaag cccagaagat gctaatcatc tagataacgt ctccttccaa
     7741 agcccgatta ccgaacagat caacatgtcg ttcattctca gcctctatga tgaagccaat
     7801 caagaaagac ttgagaccgc ttatgagaac tattacggaa ccgcttgggt cgcttccgaa
     7861 gattatgaat cctgcggtga tcaattcgtc aacgaattcg tcgctaatat ttccagatgg
     7921 cactccagcg atggcacagc catgaagaaa acccaatacg aagaaaccgc tcaagaaggt
     7981 gtcgatccat tcgtctatta tggcgcgttc gccaagatga aagacgccgc cggagccgcc
     8041 tatggtgaag aaaccgccat ggtcaccgtt gactgggata aggaaattga tggctttaac
     8101 ggatttatgt acgcgatgta cagccaaatc gttaaaaacg ccgaacatcc ctatacagcc
     8161 tgcttatacg ctcgcttcct gttaactcct gagagctacg aagcaatgtg ctataattcc
     8221 agcactccga atagccttgg cgaacccgcc aatatgtacg gctattacta tccatgcact
     8281 tcgacgaccg tcggtatcaa cgacaacgac tggtccaaag aagaatggat tgaaaagtca
     8341 attaacgaag atttcaatta cctgagcacc gtcaaaggcg cccgcatcaa cgaaatcctc
     8401 gccctcgttt ccagcaacac caaagcttcc taattatcgt taacgataac ttagccgttc
     8461 tttgaaaaaa aacgggagaa atcctttggt ctgggtttct cccgttgcta atatataggc
     8521 ggaatataat caatgaaaac aatgtccttt agcaagatat ggaacaagac aaaaacgttc
     8581 ttttccaaac ccgctaacat catcttgctt ttcttcacca ttctactgac ggcaactgtc
     8641 atctacccgc ttatttcttt agttctcgac tcatttactg tccagagcat ttccgaagct
     8701 cgcgagatta atgaaatatg gggtaccgcc gtcaaaaaag gcgactttac cttcgctcaa
     8761 tggccgaatc tcttatttaa tgccaattct gaatactcac tttacttttt ctggcagccg
     8821 ctttataaat cggtgcttat ggccttactg gcttgcgtca tcgccgttct cggcggaggc
     8881 gtactggcgt ggctcatcac cagaagcaat ctgccgatga aaaagtacat ttcggccgta
     8941 ttcatctttc cgtatatcat gccttcctgg tcgatcgcta tgttctggga aaatttcttt
     9001 aagaacacga atatcgccgc ttctaacgga accggaatct tgcaagccct gaccggcatc
     9061 tgcgtgcccg aattcctcgt ctatggaatc gtcccttgcg ctttagtgct cggcattcac
     9121 tacgccccct ttgcgtatat cttaatcggt ggaattttgc gcaacatgga cgcgaactta
     9181 gaggaagcgg cgacaatcct taaatcatca cgatttaaaa tattgagaag aattactcta
     9241 cctatcgtcg ccccggcctt aatttcgacc atcttgctcg tcttcgccag ttcgatatcg
     9301 agctacacgg tcccagcctt tttaaatgcc aacaacagtt ttacttcgat ctccatcgcc
     9361 atgcgcggtc taatcattac cgcaggcaaa aaaggacaag gctatgtcgt cgccgtcatc
     9421 ttgctggcat tcagtgtttt aattctgacg attaataact ggtttacgag atcgcggcga
     9481 agttttacga ccgtcagtgg caagtccgga caagtctcaa aattccgcat cggtaataaa
     9541 ccatggatta agtgggtcgt cgccagcatc atcgtcttgc tggtgtcttt cttcgccgtc
     9601 tttccgcttg tctccttcgt ccttgagagc cttcaggaaa catccggtaa ctatgcctca
     9661 atgactttca aatattggtt tagtcccgaa aattttgatg ttcgcatcac ggcccaaggt
     9721 cagtcttcgc aaggcatctt ccacaactac acgatttggc gtgcctttgg ccgcagtgtc
     9781 ctagtggcg
//
LOCUS       DAOSSL010000034         8679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_7724_length_8679_cov_17.020060, whole genome shotgun sequence.
ACCESSION   DAOSSL010000034 DAOSSL010000000
VERSION     DAOSSL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7724_length_8679_cov_17.020060"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1332)
                     /gene="ftsZ"
                     /locus_tag="PLH17_03930"
     CDS             complement(190..1332)
                     /gene="ftsZ"
                     /locus_tag="PLH17_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOR20951.1"
                     /translation="MQGSELDNFEPIARIVVIGVGGAGNNAVNRMIDEDLGAVEFFVA
                     NTDRQTLATSKARNRIILGPSVTGGLGAGGEPSVGREAATASVDDIRKVVKGANMVFI
                     AAGMGGGTGTGAAPVVAQVAREEGALTVAIVTRPFTFEGKKRIANSVEGLNELKDAVD
                     AIIIVSNDKLLMVSGTQPISEAFAESDRVLAQSVKTVTDLILMPAIINLDFADIRNTL
                     KDSGISLIGFGLGQGPNRAQEAAQNAINSPLLEASINGARRAICSVTCGPNVSLYEAQ
                     EAVDLVIEAAGNNIDVKFGVAINNQLTDQILVSVIASDFTEEFDFTSVPNYSQQIRLQ
                     RADGEKVNGASDDKPKKEAKEAETEAGEAASEDSILPSFLKNRHID"
     gene            complement(1343..2560)
                     /locus_tag="PLH17_03935"
     CDS             complement(1343..2560)
                     /locus_tag="PLH17_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20952.1"
                     /translation="MEKPTAAIEISSAEIKLVVGYEIDGQPIVLYAMRKPIDAFVERG
                     QIVNLIGLIDALREIKEIKDATAKLKLTISEAILLLPPLGLEVYQSDKTTNVVSPTGT
                     VEAIDISNVVSLVRKEVIPHGGQIIDIVPDAFALDNGVALSSQPLGEKSNSLSISAKI
                     HALPFSVIENFRRAFKEADIVVKRALVAPHAATYLLAQDLALPTSYLLIDMGAEVTTV
                     SLIGDRTLFSSTYFEKGGKDLTTAIMANLNLPFDEAEKLKRRYGIDHRPMSFYPTIAQ
                     SVDIEGVEKQYTTEDLNRVTEQFVYDYLVSLKQAINSLMSGYDKKYLKMPFVISGGLG
                     RLSGLDELIIEAFPDNKIHFCIPKAIGARHQTYVNTLGAILANNRYQSYLEEDRPVVT
                     PLTRGGKEAKERK"
     gene            complement(2714..2896)
                     /locus_tag="PLH17_03940"
     CDS             complement(2714..2896)
                     /locus_tag="PLH17_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR20953.1"
                     /translation="MKDKLVKFNRKAPFYRFRRAIIAFSILALVAAAISIPVSLKIAA
                     SQAEAEEADTAEVVES"
     gene            complement(2899..3840)
                     /gene="rsmH"
                     /locus_tag="PLH17_03945"
     CDS             complement(2899..3840)
                     /gene="rsmH"
                     /locus_tag="PLH17_03945"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOR20954.1"
                     /translation="MDEHIPVLLDEAIDSLGIKPNGIYVDLTLGRAGHASQILARLNR
                     KGRLIGIDQDAQAIENSKIALSKIGSNFETVHENFRNFKEVLYTLNIQKVDGILMDLG
                     VSSPQFDDSERGFSYRSNARLDMRMDERNSLTAYEIVNTYSVTDLARIFGEYGEDKYA
                     YQIARKIAEARISAPVVTTQQLVDLIKSAKPMKELAKKGHPAKQIFQALRIEVNDELG
                     ALEITIKDALKLLNVGGRLVVISFQSLEDRLIKEAFRKVTVVEGTRRNVFSLPKEEEM
                     PKFRLIHRKIVTPSAREINRNPRSRSAKLRAIERIKE"
     gene            complement(3847..4269)
                     /gene="mraZ"
                     /locus_tag="PLH17_03950"
     CDS             complement(3847..4269)
                     /gene="mraZ"
                     /locus_tag="PLH17_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOR20955.1"
                     /translation="MFFGSYSHTLDNKGRLMIPAKLRNEAGGRLYILKGYEGSLSVYK
                     EADFQNLTAALEDLSYNQRDTRDVQRITLASVVELEVDAQGRIQLPSKLLGDYKISRS
                     VVVVGVINHFEIWDAAAWENYLKQNEKDYEAKAEKLPV"
     gene            complement(4445..4951)
                     /locus_tag="PLH17_03955"
     CDS             complement(4445..4951)
                     /locus_tag="PLH17_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOR20956.1"
                     /translation="MIISKSKLILDKTIPIQKEFLFDAKEYAFKNPLLGIEKCFGDVE
                     ITKYRNYIRINVDLEVVVLLECSYTLEPFDYEFIIHEELLISDKPEDEEEAIIIPGDD
                     IDIERILFGLISSNLPLKPLKPGASLPQSGEGYRVIDDIQLAKDRTQSGDSRFSKLDD
                     IDFEDDDN"
     gene            complement(4954..7353)
                     /gene="pheT"
                     /locus_tag="PLH17_03960"
     CDS             complement(4954..7353)
                     /gene="pheT"
                     /locus_tag="PLH17_03960"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOR20957.1"
                     /translation="MKVSLNWIKKYIDIDDIAPSELADRLTFAGVEVEAVEKLSTATK
                     LVIGKVVECVPHPDSDHLSLTKVNTGEKHGVLQIVCGAPNVKAGQKVIVALDGAMLPG
                     GKISKGLIRGVESQGMICSMLELGVDAKFLTADQIAGIEVLDDDAVVGNENVLEYLGL
                     DDTILDLKLLANRSDLHSVLNVAREISTLYQREVRLPRPNQYQAVKSSFTVLSKTPLC
                     PQFSGRVIHNIKVETSPSWLKKTLQSMGVRSINNIVDIGNYVMLLSGQPLHMYDLDKL
                     PKPELIIKDDYVGSFTALDGKEYDVRKGDIAITSANQVMCLGGIMGSLASAVTEKTKN
                     IVIEVANFAFDAIRRTSSRLALASDSSSRFVKGINPYQYDEVIGFATDLIVSLCKTKQ
                     IERTVTYDARQVDQPPLIRTSVSYINNRLGTRFLQSEIVETLKRDWMNVEVLNRDDIQ
                     VGVPAWRIDIGGEADISEEVIRILGLDKIANTLPSLQVTVGGYPKDKKQINLIRRHLT
                     ARGLDEILTYSLVKAEELDGFRILTKGESHRLINPLTDEHEYMRLSLIPSMLKVASHN
                     IARGSKNLALYEVSDIDTTAEATTRLGLVLAGNDLYHHQLSEVPYSFFHLKGLIESIA
                     SLLGIKEARYSFEKLATSEQELHPGRSALLKINGQVAGYLGEIHPAATSKYELGKNKV
                     FVAEIDLPVLLSLENGQTAFKAFSRFPAVQRDLSLVAAVSVAAQQISKTILSVDRIIK
                     KVDIFDIYSGDGIEQGHKSIALTITYEREDRTLKDEEVNAVEKAVLEALNKKLGINLR
                     T"
     gene            complement(7365..8405)
                     /gene="pheS"
                     /locus_tag="PLH17_03965"
     CDS             complement(7365..8405)
                     /gene="pheS"
                     /locus_tag="PLH17_03965"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOR20958.1"
                     /translation="MEQALARLEQLKENISEEINKAASKNALVEVRNTHLSKKAEINN
                     MMALIKNFSGADKARFGQAINDIRQSLNELYDTRMRVFIDDEVSAKLASEQIDISLPG
                     INYQAGGTNPVFLVRDEILDIFISMGYEVKEGRDVETDHYNFELMNIPKNHPSRDMQD
                     SFYISENLLLRTHTSPAQARAMEEKKGEMVKVVCPGKCYRRDNDDMTHSHQFGQIEGL
                     LVGKDINLAHLKATLDLFIKKMFGPKREIRLVSSYFPFTEPSVEVQVSCAECGGKGCP
                     TCKDTGYIEILGAGMVHPNVLKMSGYDPNIYSGFAFGIGIERVAMLRYKVDDIRRFYQ
                     NDVRFLKQFVRK"
BASE COUNT         2090 a         1993 c         2217 g         2379 t
ORIGIN      
        1 ggatctgaag atgtgctcga atcgctgtcg ctagaggtat cggatgacga gtcgccggat
       61 gtatctgatg gcgatgaaat ttctgtatcg ctttgcgaaa tcgtatcgga aggcgtttgg
      121 tcacatgagg taatgacaaa agtcataacc agcgcaagaa gaagagcgac tttattattc
      181 ggtctatggt tagtcaatgt gtctattttt gagaaaactg ggcaagatcg aatcttcgct
      241 tgcggcctca ccggcctcgg tttctgcttc ctttgcttct tttttcggct tgtcgtcact
      301 ggcgccgtta actttttcgc catcagcgcg ctgcaagcgg atttgctgtg agtaattcgg
      361 gacactggtg aaatcaaatt cttcggtgaa atcgctggcg atgacgctga cgagaatctg
      421 atcggtcagc tgattgttga tggcgacgcc gaatttgaca tcgatattat tgccggccgc
      481 ttcgatgact aaatcgacgg cttcctgagc ttcatagagg ctcacattcg gtccgcacgt
      541 caccgagcaa atcgcccggc gggcgccgtt tatgctcgct tccaatagag gcgaattaat
      601 cgcattctga gcagcctcct gcgcgcggtt aggtccttga cctaagccaa agccgattaa
      661 agagatgccc gagtctttta aagtattgcg gatatcggcg aaatcaaggt tgataatcgc
      721 cggcattaag attaaatcgg taaccgtttt gacagactgg gccaagacgc ggtccgattc
      781 ggcaaacgct tcggaaatcg gctgtgtgcc gctgaccatt aaaagtttgt cgttagagac
      841 gatgataatc gcgtcgacgg catcttttaa ttcgttgaga ccttcgacgg aattggcaat
      901 tctctttttg ccttcaaaag taaaaggacg agtgacgatg gcgacggtca aagccccctc
      961 ttcgcgggcg acctgagcga cgacgggagc ggcacctgtg ccggtgcctc cgcccatgcc
     1021 ggcggcgata aagaccatgt tcgcgccttt gacgactttg cgaatatcat cgaccgaagc
     1081 agttgccgct tcgcgtccga cgctcggctc accgccggca cctaatccgc cggtgaccga
     1141 aggaccgagg atgatgcggt tgcgcgcttt agaagtcgcg agtgtctggc gatcggtatt
     1201 ggcgacgaaa aattcgacgg cgcctaaatc ttcatcaatc atacgattga cggcgttatt
     1261 tccggctccg ccgacaccga tgacgacgat gcgggcgatc ggttcgaaat tgtctagttc
     1321 ggatccttgc atatgagacc tcctatttgc gttccttggc ttcttttccg ccgcgggtca
     1381 aaggtgtgac gacagggcga tcttcttcga gataactctg atagcggttg ttggcgagaa
     1441 tggccccgag cgtattgaca tacgtctggt ggcgcgcgcc gatggctttg ggaatgcaga
     1501 aatgtatttt attgtcggga aatgcttcga taatcagttc gtccagcccg gagaggcggc
     1561 cgagacctcc gctgatgaca aacggcattt tcaaatactt cttgtcatat ccggacatca
     1621 aggagttaat cgcctgtttt aatgatacta aataatcgta gacgaactgc tccgtcacgc
     1681 gattcaaatc ttcggttgta tactgtttct cgacaccttc aatatcaacg ctttgggcga
     1741 tggttggata aaatgacatc gggcgatggt cgatgccata acggcgcttg agtttttcgg
     1801 cttcgtcaaa gggaagattg agattggcca tgatcgccgt cgttaaatcc ttgccgcctt
     1861 tttcaaaata tgtcgaggaa aataacgtgc ggtcgccaat caggctgacc gtcgtcacct
     1921 cggctcccat atctattaat agataagaag tcggaagcgc caaatcctgg gccagcaaat
     1981 aggtcgccgc atggggggcg acgagggcgc gtttgacgac gatgtcggct tctttaaagg
     2041 cccggcggaa gttttcgatg acggagaaag gcaaggcatg aatcttcgcc gagatcgata
     2101 gcgagttcga tttctcaccg agaggctgcg aagagagtgc gacgccgtta tcgagggcaa
     2161 aggcatcggg tacgatatcg ataatctgtc cgccgtgggg aataacttcc ttgcgaacca
     2221 atgagacgac attagaaatg tcaatcgcct caactgtgcc agttggagat acgacatttg
     2281 tcgttttatc gctttgatat acttcgagtc cgagcggcgg caaaaggaga attgcttcgg
     2341 agatcgtcag ttttaatttt gccgtcgcat ctttgatttc tttaatctcg cgaagggcat
     2401 cgatcagacc gattaaattg acaatttggc cgcgctcgac aaaagcatca atcggcttac
     2461 gcatagcgta aagaacgatc ggctgcccat cgatttcata gccgacaacc aatttgattt
     2521 cggccgagga tatttctatt gcggcggtag gtttttccat atcaggataa ttctgacttt
     2581 aattatatat atataaatgg cttgtggcaa aggcaatttg cacaaatatc tcccaaataa
     2641 atactaatca aagaaaaagc cgcatcataa gatgcagctc ggatggaggg aggtccgtcc
     2701 atgatgatcg attttatgat tcgacgactt cggccgtatc ggcttcttcg gcctcggcct
     2761 ggctggcagc aatcttcagg ctgacgggaa tagaaatagc ggctgccact agcgccaaaa
     2821 tcgaaaaggc gatgatcgct cgacgaaagc gataaaacgg ggccttgcga ttaaatttga
     2881 cgagtttgtc tttcatttct actccttgat gcgttcaatc gcccgtaatt tagcgcttcg
     2941 cgagcgcgga ttgcgattga tttcgcgagc tgatggggtc actatcttgc gatggatgag
     3001 gcggaatttc ggcatctctt cttcctttgg aagcgagaat acattgcgcc ttgttccttc
     3061 gacgaccgtc actttgcgaa aagcttcttt aatcagacgg tcttcgagcg attggaagga
     3121 aataacgact agccgacctc cgacattcag gagtttaagt gcgtccttaa tcgtgatctc
     3181 aagggctccc agttcatcat ttacttcgat acgcagggct tgaaatatct gcttggcggg
     3241 atgtcctttt ttagccagtt ccttcatcgg cttagccgac ttgatgaggt caactagttg
     3301 ctgggttgtg acgacgggtg cgctgatgcg cgcctcggct attttccggg cgatttgata
     3361 tgcgtattta tcttcgccgt attctccgaa gatgcgcgct aagtccgtga cgctataagt
     3421 attgacaatc tcataggccg ttaatgaatt gcgctcatcc atccgcatgt cgaggcgggc
     3481 gttgctgcga taggagaagc cgcgctccga gtcatcgaat tggggagagg agacaccgag
     3541 atccatcaag atcccatcaa ctttttgaat gttgagtgtg tatagaactt ctttgaagtt
     3601 acgaaagttt tcgtgaactg tctcgaagtt gctaccgatt ttggagaggg cgattttgga
     3661 attttcaatc gcttgcgcgt cttggtcaat tccgatcagt cggcccttgc ggtttaagcg
     3721 ggcgaggatt tggctggcgt gtccggctcg tccgagggtg aggtcgacat atatgccatt
     3781 gggtttgatg ccgagtgaat caatggcttc gtctaataag acgggaatgt gttcgtccat
     3841 agtcgattaa acgggaagtt tttcggcttt ggcttcgtaa tctttttcgt tctgtttaag
     3901 atagttctcc caagcggcgg cgtcccagat ttcaaagtgg ttgatgacgc cgacaacgac
     3961 gacacttcgc gaaatcttgt aatcgcctaa taatttgctc ggaagctgaa tgcggccttg
     4021 agcgtcgact tctaactcca cgacactcgc cagggtgatg cgttgcacat cacgggtgtc
     4081 gcgctgattg tacgagagat cttctaatgc ggcggtcaag ttctgaaagt cagcctcttt
     4141 atagacgctg agcgagcctt catagccctt gagaatataa aggcggccgc cggcttcatt
     4201 tctgagcttg gcgggaatca tcaggcgccc tttattgtca agagtgtgtg aatagcttcc
     4261 aaagaacata tttaccactt acctccactt cgtaccactt agcacccata ttatacgcac
     4321 aacgttcaaa gcgtgtaaac agcgaaaaaa gtcctaacgc ggaggtcagg accatgaaga
     4381 tttcaaatgt ttaatatatt aaacaatgaa aatatattag tagttattta gttgtatttt
     4441 ggcattaatt atcgtcgtct tcgaagtcaa tatcatcgag tttagaaaag cgtgaatcac
     4501 cactttgggt tcgatctttg gctaattgga tgtcgtcgat gacgcgatat ccttcgccgc
     4561 tctgtggaag gctcgccccc ggcttaagcg gcttaagtgg cagatttgag gagattaaac
     4621 caaatagaat cctctcgatg tcaatgtcgt cgccagggat aataatagct tcttcttcgt
     4681 cttcgggttt atcgctgatt aaaagttcct catgaattat aaattcataa tcaaaaggtt
     4741 ccaaagtata tgaacactcg agaaggacga cgacttcaag gtcgacattt atgcgaatgt
     4801 aattacgata cttagttatc tcgacatctc caaagcactt ctcgataccg agcaaaggat
     4861 ttttaaaagc atattctttt gcatcaaaaa gaaattcttt ttggatagga atcgttttat
     4921 cgaggatgag ctttgattta gaaatgatca tcgttaggtt ctcagattaa ttcccagctt
     4981 cttatttaat gcctcaagca cggctttttc caccgcattt acttcttcgt cttttaaggt
     5041 gcggtcttcg cgttcataag taatcgtaag agcgattgac ttgtggcctt gttcaatgcc
     5101 atcaccgcta taaatatcaa agatatcgac ttttttgatg atgcggtcga cggaaagaat
     5161 tgttttgctg atctgctgag cggcgaccga cacggcggcg acgagggaaa gatcgcgctg
     5221 aacggcggga aaccgcgaga aagccttaaa ggctgtctgc ccattttcta gagagagtaa
     5281 aaccgggaga tcgatttcgg ctacaaaaac tttgtttttg cctagttcat acttgctggt
     5341 ggcggcggga tgaatctctc cgagatagcc ggccacctga ccgttaattt ttaaaagcgc
     5401 ggagcggccc gggtgcaatt cttgttctga tgtggcaagc ttttcgaagc tgtatcttgc
     5461 ctctttaatg ccaagaagac tagcaatgct ctcgattaga cctttgagat ggaagaaaga
     5521 ataaggcact tcgctgagct gatgatgata gagatcgttg ccggcgagga cgagaccgag
     5581 gcgcgtcgtg gcttcggcgg tcgtatcgat atcgctcact tcgtaaaggg cgagattttt
     5641 gctgccgcga gcgatgttgt gactcgccac cttcagcatc gatgggataa ggctcagccg
     5701 catgtattca tgctcgtcgg tcagcggatt aatcaggcgg tgtgattctc ctttagtgag
     5761 aattctaaag ccgtcgagtt cttcagcttt caccagtgaa taggtcagga tttcatcgag
     5821 gcctcgagca gttagatgcc gacgaattaa gttaatttgt tttttatcct ttggataacc
     5881 tccgaccgta acttgtaagg aagggagagt gttggcaatt ttatcgaggc cgagaatgcg
     5941 aataacttcc tcgctgatgt cggcttcacc accgatatcg atgcgccagg cgggcacccc
     6001 gacttgaata tcatcgcgat ttaagacttc gacattcatc cagtcgcgct ttagcgtctc
     6061 gacgatttcc gactgcaaga aacgagttcc gaggcggttg ttgatatacg acaccgaagt
     6121 tctgataaga ggcggttgat cgacttggcg ggcatcgtaa gtgacggtcc gttcgatttg
     6181 ttttgttttg caaaggctga cgatcaaatc cgtggcaaac ccgatcactt catcgtattg
     6241 atatgggttg atgcctttga caaaccgcga tgaagaatcg ctggctaaag cgaggcggct
     6301 cgaagtccgg cgaatggcat cgaaagcaaa gtttgcgact tcgatgacga tgtttttcgt
     6361 cttttcggtc acggctgaag ccaaagaacc catgatgccg cccagacaca tgacttggtt
     6421 cgcgctcgtg atggcaatat cgccttttcg gacatcgtac tccttgccgt ctaaagcggt
     6481 gaatgaaccg acataatcat ctttgatgat gagttccggt ttcggcagct tatcaagatc
     6541 gtacatatga aggggctgac cgcttaaaag catcacgtaa ttgccgatat cgacgatgtt
     6601 atttatcgag cgaacgccca tgctctgcag agtctttttg agccatgagg gcgaggtctc
     6661 gactttgata ttgtgaatga cgcgtcctga aaattgagga caaagaggtg tttttgataa
     6721 gacggtgaat gacgacttga ccgcttgata ttgattgggg cgcgggaggc gaacttctct
     6781 ttgataaaga gtggagattt cgcgagccac attgaggaca ctgtgcaagt cgctgcggtt
     6841 ggcgaggagt ttcaaatcaa ggattgtgtc atcgagtccg aggtattcta aaacattctc
     6901 atttccgacc accgcatcat cgtcgagcac ttcgatgccg gcgatttgat cggcggttaa
     6961 aaacttggcg tcgacgccga gctcgagcat cgagcaaatc atgccctgac tttcgacgcc
     7021 gcgaatgaga cctttggaaa ttttgccgcc ggggagcatc gctccatcca aggcgacgat
     7081 gactttctgg cccgctttga cgttgggcgc accgcagaca atttgcagaa cgccatgttt
     7141 ttcaccggta tttactttag taagtgacag gtgatcgcta tcgggatgag gaacgcattc
     7201 gacgaccttg ccaatcacga gtttggtggc ggtcgaaagc ttttcaaccg cttccacctc
     7261 gacgcccgca aacgtcaagc ggtcggcgag ttcacttggg gcgatatcat caatgtcgat
     7321 gtatttttta atccaattaa gactgacttt catgttttca ccctctactt tctgacgaat
     7381 tgctttaaga agcggacatc gttttgataa aaacgccgaa tgtcatcgac tttatagcgg
     7441 agcatggcga cgcgttcgat gccgatgcca aaagcaaatc cggaatagat gttgggatca
     7501 taacccgaca ttttcaagac attgggatga accatgccgg caccgaggat ttcgatatag
     7561 ccggtgtctt tgcacgtcgg gcagcccttg ccgccgcact cagcgcaaga tacctggact
     7621 tcgaccgatg gttccgtaaa cggaaaatag gagctgacaa ggcggatttc ccgcttcggt
     7681 ccaaacattt ttttaatgaa tagatcaagg gttgctttca ggtgggcgag attgatatct
     7741 ttgccgacga gcaagccttc aatttgcccg aattggtgcg aatgggtcat gtcgtcatta
     7801 tcgcggcgat agcacttgcc ggggcagacg actttaacca tctcgccttt tttctcttcc
     7861 atggcccggg cctgagccgg agaggtgtgc gttctcaata agaggttttc gctgatgtaa
     7921 aaagagtcct gcatatcgcg ggaggggtgg ttcttgggga tgttcatcaa ctcgaaatta
     7981 taatggtcgg tctcgacatc gcgcccttct tttacttcgt aacccatgct gatgaagatg
     8041 tctaaaatct catcgcggac gaggaagacg ggatttgttc cgccggcttg ataattgatg
     8101 cccggcaggg aaatatcgat ttgttcgctg gccagtttgg cggatacctc atcatcgata
     8161 aaaacccgca ttctggtgtc atacaattca ttcagagatt gccgaatatc gttgatggcc
     8221 tggccgaagc gggctttgtc cgcgcccgaa aaattcttga tcagcgccat catgttattg
     8281 atttcggctt ttttggaaag gtgcgtgttg cgcacttcca ccaaggcatt tttgctggca
     8341 gctttattga tttcttcgct gatattctct tttaattgct ctagtcttgc gagtgcttgt
     8401 tccatcgcct gtcctcctaa aaaaataatg acacttcagg aacgaatttc tccgtggtgc
     8461 catccttctt cgcaactatt gctgcgcact aaatcttaac gcgattaacg ggacttaatc
     8521 catactcaga ggtgaattcg ccgccataat tagagatggg tcgcactcat tccatcatcc
     8581 ctggctaact atattaacgg ctactatttc tcatcaacgt acgagatatt ataacataat
     8641 cgaaaaatga tgcctatatg tatcaaatcg tattttgct
//
LOCUS       DAOSSL010000035         8237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_8225_length_8237_cov_16.790271, whole genome shotgun sequence.
ACCESSION   DAOSSL010000035 DAOSSL010000000
VERSION     DAOSSL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_8237_cov_16.790271"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..383
                     /gene="rpsT"
                     /locus_tag="PLH17_03970"
     CDS             117..383
                     /gene="rpsT"
                     /locus_tag="PLH17_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOR20959.1"
                     /translation="MAQIKSQIKRIGTNEKARIRNQAFRSKVRTQVKRFKASVDAKDA
                     VKAKEELAKSVSLIDQAVSKNVMHKNAGARQKSHLQTLFNSLQA"
     gene            complement(380..1342)
                     /gene="holA"
                     /locus_tag="PLH17_03975"
     CDS             complement(380..1342)
                     /gene="holA"
                     /locus_tag="PLH17_03975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOR20960.1"
                     /translation="MIFLIAGSQSPILKKQLNRLLGERLSAIDDFNLSRLDGREVLAQ
                     DIAYEASLPAIGAPRKAVVIENPYFLLKDKGKNKIDKEQKYQPLVEIIENPNEEIDLI
                     FVSDSVNLDEKSEVVKAIRKKGEINIIPELDASRWFQYGKSYFRSQKAIIDNDALREL
                     IERVNGDLNAFINEANKLILYKNQINLIDVTLMVSKPLENNIFAMTKALFRGDKAAAL
                     DIFSDLRTNNVEPITLVSMLANQFRLMSQIRYLDAQGYSLGEIAKRLGMPDFRIRMSL
                     QDSKKLSRFAITRNLDTLYNLDLNIKSGLIDRYYAFELFILNFA"
     gene            complement(1360..>1959)
                     /locus_tag="PLH17_03980"
     CDS             complement(1360..>1959)
                     /locus_tag="PLH17_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR20961.1"
                     /translation="RGKNVLIDTGGNKSFDMAKEVLIPFFKKKRVYKLDYLITTHDDF
                     DHRGAAPSLLENFRVEHIVANAHAFPLALGTTIFRNLNDTLSADENDNSLVLTFTLLD
                     KEWLLMGDASKTVEERIISRNPNLNIDYLKIGHHGSNTSTGESFLRHTTPEEAIISVG
                     SDNYYGHPHQEVIALLHRYDITIRRTDLEGTISYSSALF"
     assembly_gap    1960..2059
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2060..3533)
                     /locus_tag="PLH17_03985"
     CDS             complement(<2060..3533)
                     /locus_tag="PLH17_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOR20962.1"
                     /translation="MIAFLILSCLVLGLWLFRMPWPASLILLLIGVGFAFYRFKGRGL
                     LIFVLATMGGWAITNLDRIEPPNQETYRGFVVSSKENYFLIQSGAHKFYVQEDDNHRE
                     LGDYLEIDGYLKSLSFPAYESRFDFQGYLNDKGVFHELRVLKTEVLFTNPLRLNARKD
                     AFLNNFDDTSADAIDALLFSRKNYEAAMIQNAEAMNILFLFSMCGIYLRLAMSICEYF
                     LKLKLTDKAARITTLILLLPLFLLGLEKMSTHRLFLSYGGRIINDYFLKKKVSYLTLL
                     STLALGFLALDFHAAFSLAFLLGFGLSALIVFLRSAMRRFKRKYRRLVTGLFIFLFLV
                     PVHLSMSAELHFLQILFQYIALPLNAFFFFAAATSFYTWPMRHLLPFLARLMTSVYTF
                     LRKVDIVMIVGEPPLFLYFVYYGLYFYIPYLLESHRQSDAKKVAVALSSCLVLAALPI
                     HNYLGEAIHFINVGQGDAILVQTRGKNVLIDTGGNKSFDMA"
     gene            complement(3530..4057)
                     /locus_tag="PLH17_03990"
     CDS             complement(3530..4057)
                     /locus_tag="PLH17_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HOR20963.1"
                     /translation="MIKIIIGVIVAAIILIVILQSIDPDVGGGTTNTAEVYEMLTVSV
                     SGEVVKPGTYILELDTDLADLIDAASGVTSNADELAFDLTYLLENNQSFYIAPKYDMS
                     DVCAIEPINKVNINTDSKETLMSVNGFGSAISDAVIAYRADHQFKRIEDIKNVSGIGN
                     ATFEKIKNYITIRSA"
     gene            complement(4158..4634)
                     /gene="greA"
                     /locus_tag="PLH17_03995"
     CDS             complement(4158..4634)
                     /gene="greA"
                     /locus_tag="PLH17_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOR20964.1"
                     /translation="MVDNKLILTKDGVKKLKEELRHLIDVERPEVIEQLTFARSQGDL
                     SENADYDAARVRQAEIEGRIKQIEDILATAKVIKDKPKNTKCVTIGSKVHIRDLGLNA
                     EKVYTIVGTVEADPFAGRISNDSLLGSALIGKQVGDMVTVKTTKPYEVEILRIDNA"
     gene            complement(4745..5002)
                     /locus_tag="PLH17_04000"
     CDS             complement(4745..5002)
                     /locus_tag="PLH17_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOR20965.1"
                     /translation="MDEQDKKLVIQDEDGKRYEREILFTYENAENHKKYVFFFDPKAK
                     DEEVQVLRYADDGTLEEVESDEEYEQLEEVFNSYQADEEEK"
     gene            complement(4975..5427)
                     /gene="ruvX"
                     /locus_tag="PLH17_04005"
     CDS             complement(4975..5427)
                     /gene="ruvX"
                     /locus_tag="PLH17_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOR20966.1"
                     /translation="MKKIIALDLGTKTLGIAITDGLLIAAHGYENYTFEDGNYKKAHQ
                     HVLDILSKEGVEELAIGLPLHMSGAASARSESALRFRDDLLSERPDLKIAMVDERMTS
                     IIANKRLLEADLSRNKRKAVIDKMSAVVILESYLQMRDQPWMNKTKNS"
     gene            complement(5424..8033)
                     /gene="alaS"
                     /locus_tag="PLH17_04010"
     CDS             complement(5424..8033)
                     /gene="alaS"
                     /locus_tag="PLH17_04010"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOR20967.1"
                     /translation="MKKLTGHEIRKIWINFFEERGHKFEKGASLIPYKDPTLLWINSG
                     VAGLKKYFDGSEIPPSRRIVNVQKSIRTNDIENVGYTARHHTFFEMLGNFSIGDYFRQ
                     EMLPWAYEILTSDKYFGMPKDKLYFTYLPTDSATRELWLKCGVASDHLIPLSGNFWQI
                     GEGPCGPNTEVFFDRGETYDVEKKGIALLANDLENDRYIEIWGIVFSQFNAVDGHKRE
                     DYKELPSKSIDTGAGLERIACVLQETPTNFETDLFFPLIKKAEELASEPYRGVNLTAY
                     RVIADHIRTVVFALSDGEPFSNEGRGYVLRRVLRRAMRFGRKIGIDKPFLYTLVKTVV
                     EMMKDFYPELAKRQDYVERSVKAEEEKFLKTLKGGEVMLRDMLIGKKVLSGEDAFRLY
                     DTFGFPVELTIEIAQESGVKVDLEGFKEEMNKQKERARQARSDAGSMGQQHSDLLKFV
                     APSIFSYAEGDIQAKVIGLFANGGAVQDLVGEGEVVFDVTNFYAESGGQIGDSGRIYG
                     PDFEARVLNTRKAPNKQHLHEVNISFGTLHIGDVLTLEIDHARRHLTMRNHSATHLLH
                     SVLAGVLGDHITQQGSFVSDEYLRFDFSHYAKISDEQLLLIEKQVNELIASSLNSNVR
                     ILPLKEAEKTGAKAFFSEKYGDEVRVVAFGDVSQEFCGGTHVHNTEEIGLFVIESEES
                     IAAGVRRIQARTSLGAYHLLKRREVLLHKAQNLVGASSIHEVSDRLKAMLNYEQELKE
                     ENIRLTDKIVAVTADSLRHSISVYGGYHVVFKHLTGLKREVLVKIGDNLKAHYQDYVL
                     VLAGGESDNVALVAFVGGQALKDGVKAGDIIREMSKQIGGSGGGRAEMAQGQGKNLAA
                     LSKAFDKIKESLK"
BASE COUNT         2120 a         1831 c         2018 g         2168 t
ORIGIN      
        1 agatggtctt gacttaccgc ggcaagaaga gcaaataacg agcggaacga ataacaatat
       61 ttatattgtc atcactgaag gtatttgata tagttttagc gttaagaggt aataaaatgg
      121 cacaaattaa atcacagatt aaacgtattg gcacgaatga aaaggcccgc atccgcaatc
      181 aagcctttcg cagcaaagtc agaactcaag tcaagagatt caaggcttct gtcgacgcca
      241 aagacgccgt caaagccaag gaagaactcg ctaagagcgt ctctttaatc gatcaggccg
      301 tcagcaaaaa tgtcatgcac aaaaatgccg gagcccgtca aaagagtcat cttcaaacgc
      361 ttttcaattc tttacaagct taagcgaaat taagaataaa cagctcaaaa gcataatagc
      421 ggtcgatgag accgcttttt atgttgagat caagattata caaagtatcg aggttgcggg
      481 tgatggcaaa gcgcgagagt ttcttggagt cttgaagcga catgcggatg cgaaaatccg
      541 gcattccgag ccgcttggca atctctccta aggagtatcc ctgagcgtcg agatagcgaa
      601 tctggctcat caggcgaaac tggttggcca gcatagacac gagcgtgatt ggctcgacat
      661 tgttcgtcct gagatcggag aagatgtcta aagccgcggc cttatcgccg cgaaaaagag
      721 cttttgtcat cgcgaaaata ttgttttcaa ggggtttaga caccatcagc gtcacatcga
      781 tgaggttgat ttgattttta tacaaaatga gcttatttgc ttcgttgata aacgcgttca
      841 ggtcaccatt aacacgttcg atcaactcgc ggagggcgtc attgtcgatg atggcttttt
      901 ggctgcgaaa atatgacttg ccgtattgaa accagcggct ggcatcgagt tcggggatga
      961 tattgatctc accctttttt ctaatggctt tgacaacttc actcttttca tcgagattaa
     1021 cgctgtcaga aacgaagatg agatcgattt cctcgttggg attttcgatg atttcaacca
     1081 aaggttgata cttctgctct ttatcgattt tattcttgcc cttatccttt aaaagaaaat
     1141 aggggttttc gatgacgacg gccttgcgcg gcgcgccgat ggcaggcaag ctcgcctcat
     1201 aggcgatatc ttgagcgaga acctcacgtc catccaggcg cgagagattg aaatcgtcga
     1261 tggctgaaag ccgctcgccg agcaagcggt tcaactgctt tttgagaatt ggcgattgtg
     1321 agccggcaat tagaaaaatc atcttgcttt gattatatat caaaagagag ccgatgagta
     1381 cgaaatcgtt ccttcgagat cggtccggcg gatggtgatg tcatagcgat ggagcaaagc
     1441 gatgacttcc tgatggggat gaccataata attgtcgctt ccgacggaga tgatcgcttc
     1501 ctcaggtgtt gtgtgtctta aaaaactttc acccgttgaa gtatttgatc cgtgatggcc
     1561 gattttcaga taatcgatat tgagattcgg attgcgactg attattcgtt cttcaaccgt
     1621 tttggaggcg tcacccatca agagccactc tttgtctaga agggtaaaag tcaaaacgag
     1681 cgagttgtcg ttttcatcgg ccgataaagt atcattgaga tttcggaaaa tggtagttcc
     1741 gagcgcgagt ggaaaggcgt gagcgtttgc cacgatatgt tcgacgcgga agttttcaag
     1801 caaagacggt gccgcgccgc gatgatcaaa atcgtcatga gtcgtgatta aataatcgag
     1861 tttataaact cttttctttt taaagaaagg aatgaggact tcttttgcca tgtcgaacga
     1921 cttgtttccg ccggtgtcga tgaggacatt tttgccgcgn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnt tgccatgtcg aacgacttgt ttccgccggt gtcgatgagg
     2101 acatttttgc cgcgcgtttg gacgagaatc gcgtcgccct ggccgacatt gataaaatgg
     2161 atggcttcac cgagatagtt atgaatcgga agagcggcta agacgaggca agaagacaga
     2221 gccacggcaa cttttttcgc gtcgctttgc cgatggcttt ccaaaagata ggggatataa
     2281 aaatagagtc cgtaataaac aaaatacaaa aatagaggcg gttcgccgac aatcatgacg
     2341 atgtcgactt tcctgagaaa ggtataaacc gaggtcatta gtcgcgccaa aaaaggcagg
     2401 agatggcgca tcggccatgt gtaaaaagag gtggcggcgg caaaaaagaa gaaagcgttg
     2461 agcgggagag caatatattg aaaaagaatt tgcagaaagt ggagttcggc actcatcgaa
     2521 aggtgaaccg gaactaagaa aaggaagatg aaaagccccg tcaccagccg ccggtatttg
     2581 cgcttgaagc gacgcattgc cgatcgtaaa aaaacgataa gcgcggaaag gccgaaaccg
     2641 aggagaaaag ccaaggaaaa agctgcgtga aagtcgaggg caagaaaccc cagggccaag
     2701 gtggacaaaa gggtgagata tgatactttt ttcttaagaa aatagtcgtt gataattcgc
     2761 cctccataac ttaaaaacaa gcgatgggtc gacatttttt caagacctaa aagaaaaagc
     2821 ggaaggagga gaattaaggt tgttatccgc gcggccttgt cggtcaattt cagcttcaga
     2881 aagtattcgc atatcgacat tgccaagcgc agatagatac cgcacatgga aaagagaaaa
     2941 aggatgttca tcgcctcggc gttttgaatc attgcggctt cgtaattttt ccgcgaaaaa
     3001 agcagggcgt caatcgcatc ggcactcgta tcatcaaagt tatttaaaaa ggcatccttg
     3061 cgggcattca ggcgcaaggg attagtaaaa agaacctcgg tttttaaaac cctcaactca
     3121 tggaagacac ccttgtcatt taaataaccc tgaaaatcaa agcggctttc ataggcggga
     3181 aacgataacg acttaagata gccatcaatt tctaggtagt cgccgagttc gcgatgatta
     3241 tcgtcctcct gaacgtaaaa cttgtgagcg ccactttgaa taaggaagta attttctttg
     3301 ctggagacga caaacccccg ataggtctct tggttcggcg gttcaatgcg atcgagatta
     3361 gtaatggccc agccgcccat cgtcgcgaga acaaagatga gcaagcctcg tcctttaaag
     3421 cgataaaaag caaagccgac accgatgaga agaagaataa gcgaagcggg ccacggcatc
     3481 cggaaaagcc ataatcccag gacgagacaa gaaaggatca gaaaggcgat catgccgagc
     3541 ggatcgtgat gtagttttta attttttcaa atgtcgcgtt gccgatgccg gagacatttt
     3601 tgatatcttc tatgcgcttg aactggtggt cggcgcgata ggcgatgaca gcatcggaaa
     3661 tggccgagcc aaaaccgttg acactcatga gtgtttcctt gctgtcggtg ttgatattaa
     3721 ctttgttaat cggctcgatt gcgcacacat cgctcatgtc gtatttggga gcgatgtaaa
     3781 aggactgatt gttctctaat aaataagtta ggtcaaacgc caattcgtcg gcgttgctcg
     3841 tcacgcccga agccgcgtcg atcaaatcgg ccaggtccgt atcgagttct aagatataag
     3901 tccccggttt gacgacttca ccggagacgg agacggtcag catctcatag acttcggcgg
     3961 tgttcgtcgt gcctcctccg acatcgggat cgatgctttg caagatgacg attaagatga
     4021 tggcggcaac gatgacgccg atgatgattt taatcataaa tttcctccta ccgatttata
     4081 agcaagaaga aagcaataac ttccaaacaa taaaaaaacg gaagttacct tccgcttcgt
     4141 ttagagtttt gtcttattta agcgttatcg atgcgcagga tttcgacttc gtaaggcttg
     4201 gtcgtcttca ccgtcaccat atcgccgact tgcttgccga tcaaggcgct tcccaatagc
     4261 gaatcgttgg aaatgcgtcc ggcaaacgga tcggcttcga cggtcccgac gatcgtatat
     4321 accttttcgg cgttaagtcc caaatcgcga atgtggactt tcgaaccgat cgtcacgcat
     4381 ttcgtatttt tgggcttatc tttgataact ttggcggtcg ctaaaatatc ttcgatttgc
     4441 ttaatgcgtc cttcgatttc ggcctggcgg acgcgagcgg catcgtaatc ggcattttcg
     4501 ctcagatcgc cttgcgagcg agcaaaggtt aattgttcga tgacttccgg gcgctcgacg
     4561 tcgattaagt ggcggagttc ttctttgagc ttcttgacgc catcttttgt taaaatgagt
     4621 ttattgtcta ccatatgtgc tacctctttg tcagcgaatt agattataac aacatcatcc
     4681 cgagattgct acaataaaaa ttacgctgct tttctatata aatagaataa taaaaataat
     4741 acaactattt ttcttcttcg tcggcctgat aagaattaaa gacttcctct aattgctcgt
     4801 attcttcgtc gctttcgacc tcttcgagcg tgccgtcgtc agcgtagcgc aaaacctgaa
     4861 cttcttcatc cttggctttg gggtcgaaaa agaagacgta cttcttatgg ttttccgcgt
     4921 tttcgtaagt gaagagaatc tcgcgttcgt agcgctttcc gtcctcgtct tgaattacga
     4981 gttttttgtc ttgttcatcc atggttgatc cctcatttgt aagtatgatt cgagtatgac
     5041 gaccgccgac atcttgtcga tgacggcctt gcgtttattt cttgataagt cggcctctaa
     5101 gaggcgcttg ttggcgatga tgctcgtcat ccgctcgtcg accatcgcga ttttgaggtc
     5161 gggccgttcg cttaagaggt catcgcgaaa gcggagggct gattccgagc gagccgaagc
     5221 ggcacccgac atatgaagcg ggaggccgat tgccaactct tcgacacctt ctttgcttaa
     5281 gatatctaaa acgtgctggt gggccttttt atagttgccg tcttcaaagg tgtagttctc
     5341 atagccgtgg gcggcgatta aaaggccatc ggtgatggcg atgccgagcg ttttcgtgcc
     5401 gagatcaagg gcgattatct tcttcatttc aaactttctt tgattttatc aaatgctttg
     5461 gataaagccg ctaaattctt cccttgcccc tgggccattt ctgctcgtcc gccacctgag
     5521 ccgccgatct gcttactcat ttcgcgaatg atgtcgccgg ccttgacgcc gtctttcagg
     5581 gcttggcctc cgacaaaggc gacgagggcg acattatcgc tttctccgcc cgccaagacg
     5641 aggacataat cctgataatg agctttaaga ttgtcgccga ttttgacaag cacttcgcgc
     5701 tttaatcctg ttaagtgttt aaagacgaca tgataacctc cgtaaacgga gatactgtgc
     5761 cgaagcgaat cagcggtcac ggccacgatt ttatcggtta aacgaatatt ttcttcttta
     5821 agttcttgtt cgtaattaag cattgctttg aggcggtcgc tcacttcgtg gattgaagaa
     5881 gctccgacga ggttctgcgc tttgtgcagt aaaacttcgc gccgttttaa aagatgatag
     5941 gcgccgagcg acgtgcgcgc ctgaatgcgg cgcacgccgg cagcgattga ttcttccgac
     6001 tcgatgacga agagtccgat ttcctcggtg ttgtgaacat gggtgccgcc gcagaactct
     6061 tgcgagacat cgccaaaagc gacaacgcgg acttcatcgc cgtatttttc gctgaaaaag
     6121 gctttagctc ccgtcttttc cgcctctttg aggggcaaga tgcggacatt tgaattaagc
     6181 gaggaagcaa tcagttcatt aacttgtttt tcaatgagta aaagctgttc atcgctgatt
     6241 ttggcatagt gtgaaaagtc aaatcttaag tattcatcgg agacgaatga cccttgctgg
     6301 gtgatgtgat cgccgagtac gcccgcgagc acgctgtgca agaggtgcgt cgccgagtga
     6361 ttgcgcatcg ttaagtgacg ccgggcatgg tcgatttcaa gagtcagaac atcgccgata
     6421 tggagtgtgc cgaaactaat gttgacctca tgaagatgtt gtttattagg ggctttgcga
     6481 gtgttgagaa cgcgcgcttc gaaatctggg ccgtaaatgc ggcccgaatc gccgatctga
     6541 ccaccgcttt cggcgtaaaa attggtgaca tcgaaaacga cttcgccttc tccgactaag
     6601 tcctgaaccg ctccgccgtt tgcaaaaagg ccgataacct tggcttgaat atcaccttcg
     6661 gcataactga atatgctggg cgcgacaaat tttaagagat cggaatgctg ctgtcccatg
     6721 cttccggcat cgcttctggc ctgccgggcc cgttcttttt gcttattcat ttcctcttta
     6781 aaaccttcta aatcgacttt gacgccgctt tcctgggcga tttcaatcgt caactcgacg
     6841 gggaaaccaa atgtgtcata aaggcgaaaa gcatcctcgc cgcttaagac ctttttaccg
     6901 atgagcatgt cgcgaagcat gacttcgccg cctttgagcg tttttaggaa tttttcttcc
     6961 tcggctttaa cgcttctttc gacataatct tggcgtttag ctagttccgg ataaaaatct
     7021 ttcatcatct cgacaacagt cttcactaat gtataaagga aaggcttgtc gatgccgatc
     7081 ttgcggccga agcgcatcgc gcgtcttaag acgcggcgca gtacataacc gcgaccctca
     7141 tttgaaaagg gttcgccgtc agagagggca aagacaaccg tccggatgtg gtcggcgatg
     7201 acgcggtagg cggtcagatt gacgccgcga taaggctcgc tcgccaactc ttccgccttt
     7261 ttaatcagtg gaaaaaagag gtcggtttca aagttggtcg gcgtctcttg caagacgcag
     7321 gcgatgcgct ctaaaccggc gccggtatca atgcttttgc tcggcaattc tttgtaatct
     7381 tcgcgcttat gtccatcgac ggcgttaaac tgcgaaaaga cgatgcccca gatttcgatg
     7441 tagcgatcgt tttcaaggtc gtttgccaat agggcgatgc ctttcttttc gacatcgtaa
     7501 gtctcaccgc ggtcaaaaaa gacttcggtg tttggtccgc acggaccttc gccgatttgc
     7561 caaaagttac cggaaagagg aattaaatga tcgcttgcga caccgcactt gagccacaac
     7621 tcgcgcgtgg ctgaatcggt cggcaaataa gtgaaataga gtttgtcttt gggcatgcca
     7681 aaatatttgt ccgaagtgag gatctcgtat gcccatggaa gcatttcctg acggaagtaa
     7741 tcgccgatcg agaaattgcc gagcatttca aaaaaagtgt gatggcgagc cgtatagccg
     7801 acgttttcga tatcatttgt tctgattgat ttctggacgt tgacgatgcg ccgtgagggg
     7861 gggatttcgc tgccgtcaaa atatttcttg agcccggcga cgccggagtt gatccagagc
     7921 agcgtcggat ctttataagg gatgagcgat gccccttttt cgaacttgtg tccgcgctct
     7981 tcaaaaaaat taatccagat tttgcgtatt tcgtggccgg taagtttttt catcgtttgt
     8041 tcctctaaaa caaataaaaa tcgtccctaa ggaacgattc gctcgcggta ccatccttat
     8101 tcattactga taatgcactt aattaggctt taacgcagcc gacggcgggg attagtcgct
     8161 tcagaggagt ggcacgcttt tagcttagtt cctctattga atgtagtaaa tatagcatat
     8221 ttaagcaaca cttcaaa
//
LOCUS       DAOSSL010000036         7656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_8985_length_7656_cov_16.076569, whole genome shotgun sequence.
ACCESSION   DAOSSL010000036 DAOSSL010000000
VERSION     DAOSSL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8985_length_7656_cov_16.076569"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..767)
                     /locus_tag="PLH17_04015"
     CDS             complement(39..767)
                     /locus_tag="PLH17_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOR20968.1"
                     /translation="MIYQVTSKDNRRVVEAGKLKQAKFQKETGLFLAEGFNLLEMAVA
                     AGSVLYVFAKKPIAGLNVDQYIVNDDILRKLASSKTPQGVVSVCKAAPSQPIATDKVI
                     CLDDIADPGNLGTILRTALAFGYRDVALSLKCVSPYNDKALNASQGAVFKLNLVAMAS
                     KEIISLGERGYKLVATSLEGSVSLSSFKLQPPYVIILGNEAHGVSNELLKAADYRVKI
                     DISGIDSLNVAVASGIVLYELNRK"
     gene            complement(764..1414)
                     /locus_tag="PLH17_04020"
     CDS             complement(764..1414)
                     /locus_tag="PLH17_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOR20969.1"
                     /translation="MVYELTYRRDGHDYRVVVTIKQIKNVIFRFRDQVFYVSAPRRTK
                     EATIIAYLDKFYERLLAKHAQNTTPFINQQVYLLGELFDLYEGSSVKNTFIFGRGLYY
                     KNQRDFDKKLRAFAKDYYTLRVRHHEQIMKIKKPYDIKVRDMKTRFGTNSRKTHALSF
                     QLRLIHFHPLIIDAIVIHELAHDRHFDHGPSFYQELYKYCPDYKILVKRLKKGQFA"
     gene            1521..1985
                     /locus_tag="PLH17_04025"
     CDS             1521..1985
                     /locus_tag="PLH17_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOR20970.1"
                     /translation="MKKVLISACLIGDKVRYDGRDNAIEHIEQLMKHFELIPFCPEVE
                     GGLSVPRLKSEIRNGRVFNEERKEVTKYFLSGANKALNICKYMNIELAILKEDSPSCG
                     VKSVYDGAFSRRKIPGQGITAALLKRNGVEVISELDVPDLLEKLASKKDAAV"
     gene            complement(2013..3101)
                     /locus_tag="PLH17_04030"
     CDS             complement(2013..3101)
                     /locus_tag="PLH17_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOR20971.1"
                     /translation="MKQEERLQFLREMSEVEGISGREKDAAKLFRKHVEGVADRIEYD
                     NLGSVLAYKDGKKGEPIIMLSGHIDEVGFLVHRIEDSGFIRFHPIGGWWGHVVLAQTV
                     RITASDGHKIIGVVGAQPPHGMSAEQRNKVMEIKDMYIDIGVSSKEDVEKLGIRVGDM
                     ITPYTPFRVMNDGKTLLGKAWDDRLGAAVAIEVLQNLKGVDHQATVVAAGTVQEEVGL
                     RGARTAAYHVKPDVAFALDVTMSYDLPGAPNHPTKLGAGVALSIMDGSVIAHRGLFDF
                     VEKVAKEKGIKYTFDLMTAGGTDSGEIHKQYDGVVTMTISIPCRYFHSHVSLINYDDY
                     LEAVKLLTEVVKALDKDTLAAIKATKYE"
     gene            complement(3114..3611)
                     /locus_tag="PLH17_04035"
     CDS             complement(3114..3611)
                     /locus_tag="PLH17_04035"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOR20972.1"
                     /translation="MSQMIELKELYRLQGELDARIASNHKTSYEKTTDDRLMALIVEI
                     GELANETRCFKYWSNKGPSPREVLLDEYADGMHFLLSLGIPLGVRTTRYEIIESELPL
                     TRQFHLLYRATVELVESYDLAHYTNAYQIFLNLIPSLGFSVEEVVCAYKAKLDVNYQR
                     QNSNY"
     gene            complement(3608..>5239)
                     /gene="glgP"
                     /locus_tag="PLH17_04040"
     CDS             complement(3608..>5239)
                     /gene="glgP"
                     /locus_tag="PLH17_04040"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOR20973.1"
                     /translation="YPDDSTEQGRILRLRQQYFLVSAGLQSAMRGHFRRFGTLDNFAD
                     YHVFQLNDTHPILAIPEMMRLLIDEYEYEWEEAWCQVQKSFAYTNHTVMAEALEKWPI
                     QYVQRLLPRCFMIIEEISRRFNLEMAEKNIDYGKRYAMQIIKDGQIHMTNLAIHASYS
                     VNGVAKLHTEILKRDTFRDFYELYPQKFNNKTNGVTHRRWLMYANPELAALITSKIGE
                     GWIKDFSLIKDFEQYKDDKKVQDAILRIKHDNKIAFASFMKKKQNVDLNVDSIFDVQI
                     KRLHAYKRQLMNIFHIIYLYQRLRDDPSFTIFPRTFVFGAKAAPSYDYAKKIIELILA
                     VADIIDRDPHVSKFIKVLFVENYGVSSAEKIIPASDISEQISTAGKEASGTGNMKFMM
                     NGAITLGTLDGANVEIADFVGLDNCVIFGLKAEDVEGHYERGDYNPWDIYNSDERIKK
                     VIDSLFSGPWTFGKDEKYQLIFDEVMHRNDTYFILKDFAAYVEAQEKIEKLYLDRRNW
                     ARSQIINIANSGFFTSDRTIEQYVRDIWHLRKLKI"
     assembly_gap    5240..5339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5340..5761)
                     /locus_tag="PLH17_04045"
     CDS             complement(<5340..5761)
                     /locus_tag="PLH17_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOR20974.1"
                     /translation="MFKNKAEFKDEFSKRLSEKYGKNVKDSHISERYDILGELVRDYA
                     GNYWRQTREYVLKNKEKQLIYFSMEFLIGKLMHSNMMNLGIYEIAEQGLKELGIDINE
                     LEDEEHDAGLGNGGLGRLAACFMDSLASLGYAGHGNCIR"
     gene            complement(5771..7624)
                     /gene="glgB"
                     /locus_tag="PLH17_04050"
     CDS             complement(5771..7624)
                     /gene="glgB"
                     /locus_tag="PLH17_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOR20975.1"
                     /translation="MAYGLLHDFLTGNTTRAYEYFGAHFIASETEGKKERGVMFRLYA
                     PLAEDVSVIGSFNNWDVGAHKMQKVDLAGVWEVFIPGLKEYESYKYHFRNAKGQYVDK
                     ADPYAFYSEYRPATCSRLFDIENFIWHDSSYMKSRNRSFDDPMSIYEIHLGSWKGKVD
                     NRNLSYEEIADYLIPYVKELGYTHVEIMPITQYPFDGSWGYQGTGFYSVDSRYGNPKQ
                     LMSFVDRMHQAGLGVILDFAPVHFAKDEFALADFDGAYLYEYTDIKHRYSQWGSLLFD
                     LGKDPVRSFLISAMNYFASYFHFDGLRIDAVSNVIFYDGNKDKGENAGGIEFLKRMNT
                     LLHGEHSSLMTIAEDSSDYQGVTKPLEFGGLGFDYKWDLGWMNDTLKYYAKDPIYKKY
                     EHHKLTFSMAYFFSENFLLPLSHDEVVHGKGTIINKLWGDYEQKFALLRNLYTYQFGH
                     PGKKLNFMGNELASFDEWNENKSLPWNLRTFPKHDSITRLIRDLNLIYSYEKAMKFEE
                     YNPKHFLWLMADNADQSIYAFERFVDDSRLIFVFNMTPNFFDNYELGVNEEGTYEEIF
                     NSDKDVYGGANQYNGLPVPSAPFGPFNRPHHINIKIASFGAMIFKHRKNKK"
BASE COUNT         1900 a         1708 c         1875 g         2073 t
ORIGIN      
        1 caaatcgtat tttgctttaa aagcaaatta aatacttact atttgcggtt tagttcgtaa
       61 aggacgatgc cgctcgccac cgcgacattt agggaatcga tgcccgagat atcgattttg
      121 acgcggtaat cggcagcctt tagcaactcg ttgctcactc catgcgcttc gtttcccaag
      181 atgatgacat atgggggctg aagtttaaaa gatgataagg agacgcttcc ttcaagtgat
      241 gtcgccacca acttataacc gcgttcgcca agcgaaataa tttcttttga tgccatcgcg
      301 acgaggttga gtttaaaaac cgctccttgt gaggcgttca aagccttatc gttataaggc
      361 gagacacact ttaaggaaag ggctacatcg cgatagccaa aagcgagggc cgtgcgcaaa
      421 atagttccga gattaccggg gtcggcgata tcatcaagac atattacttt gtcggtcgcg
      481 atgggctggc ttggggcggc tttgcagaca gagacgacac cttggggagt ttttgaagaa
      541 gccaacttgc gaagaatgtc atcgttaacg atgtattgat caacatttaa accggcgatc
      601 ggttttttgg caaacacata taaaacggaa ccggcggcca ccgccatttc cagaagatta
      661 aatccctcgg ctaagaaaag gccggtttcc ttttgaaatt tagcctgttt gagtttgccg
      721 gcttcgacga cgcggcgatt atctttagaa gtcacttgat atatcatgcg aactgacctt
      781 tcttgagcct cttgactaag attttataat ccgggcaata tttatacaat tcctgataaa
      841 aggatgggcc gtgatcaaaa tggcggtcat gggccagctc gtgaatgacg atggcatcga
      901 tgattaaggg atgaaaatgg atgaggcgga gctgaaagct taaggcgtga gtcttgcgcg
      961 aattggttcc gaagcgcgtt ttcatatcgc gaaccttgat atcgtaaggc ttcttgattt
     1021 tcatgatttg ctcatggtgg cgaacgcgaa gcgtatagta gtccttggca aacgctcgca
     1081 gctttttatc gaaatctctc tgatttttat aatataaacc gcggccgaag ataaaggtgt
     1141 ttttgacaga cgagccctcg tagagatcaa agagttctcc gaggagataa acttgctgat
     1201 tgataaaagg ggtcgtgttt tgcgcgtgtt tggccagcaa gcgctcgtag aacttatcga
     1261 gataagcgat aatcgtcgcc tctttggtcc gcctcggagc gctgacatag aagacttgat
     1321 cacgaaagcg aaaaattaca tttttaatct gtttaattgt caccaccacg cgataatcgt
     1381 ggccatcgcg gcgatatgtc agttcgtaga ccatgctata agtataagcg catcgccgag
     1441 agttaaaaag actaaagacc gcgcataatt ctttttttat ctgttgtcgt cacagggtga
     1501 taattataca attatgaagt atgaaaaaag ttctgatcag cgcctgcctt atcggcgaca
     1561 aagtccgata cgacggaaga gataacgcaa ttgagcatat tgagcaattg atgaagcatt
     1621 ttgaactcat tcctttttgc cccgaagtcg aaggcggatt aagcgttccg cgcctcaaaa
     1681 gcgaaattcg taacggtcgc gtttttaacg aagagcgcaa agaagtcacc aaatactttt
     1741 tatcgggagc caacaaagct ctcaacatct gcaaatatat gaatattgaa ttagcaattc
     1801 tcaaagaaga ctcgccttcg tgtggcgtta aaagcgtata tgatggcgcc ttcagccgcc
     1861 gcaaaatacc cggccaaggt atcaccgccg cccttttaaa acgaaacggc gtcgaggtca
     1921 tcagcgaact ggatgttccc gatctcttag aaaaattagc aagtaaaaag gatgccgcgg
     1981 tctgatcgca acattctttt tttattaaac aactattcgt attttgtggc tttaatcgcg
     2041 gccagagtgt ccttatcgag agctttgacg acttcggtca ggagtttaac ggcctctagg
     2101 tagtcgtcgt agttgatgag cgagacatgg ctgtggaaat agcggcacgg aatcgagatc
     2161 gtcatcgtca cgacgccgtc gtactgctta tgaatttcac cggaatccgt ccctccggcc
     2221 gtcatcaggt caaaggtgta tttgatacct ttctctttag cgactttttc gacaaagtcg
     2281 aataagccgc ggtgggcgat gaccgagccg tccatgatcg ataaggcgac gccggcgccg
     2341 agtttggtcg gatgattcgg cgctcccgga agatcgtatg acatcgtcac gtcgagggca
     2401 aaggcgacat cgggtttaac atgatatgct gcggtccggg caccccgcaa accgacttct
     2461 tcctgaacgg ttccggccgc cacgacggtc gcttgatgat cgacgccttt taaattttgc
     2521 aagacttcga tggccaccgc cgctccgagg cgatcatccc aggctttgcc gaggagtgtt
     2581 ttgccatcat tcatgacgcg gaagggagtg tacggcgtaa tcatatcgcc gacgcgaatg
     2641 ccgagctttt cgacatcttc tttcgaactg acgccgatat cgatgtacat gtctttgatt
     2701 tccatgactt tattgcgctg ttcggcgctc atgccgtgcg gaggctgcgc tccgacgacg
     2761 ccgatgattt tatggccgtc tgaggccgtg atgcgcacgg tctgggcgag gacgacgtgg
     2821 ccccaccagc cgccgattgg gtgaaagcgg ataaaaccgg aatcctcaat gcgatggact
     2881 aagaaaccga cttcgtcgat gtggcccgaa agcatgatga ttggctcacc ttttttgcca
     2941 tctttataag cgagaactga accgagatta tcatactcga tgcggtcggc gacgccttcg
     3001 acgtgttttc tgaataattt agcggcgtct ttttcgcgtc cggagatgcc ttcgacttcg
     3061 ctcatttcac gtaaaaattg taatctttct tcctgtttca tatggtttac ctcctagtaa
     3121 ttgctgtttt gccgctggta attgacatcg agtttggctt tgtaggcgca aacgacttct
     3181 tcgacgctga aaccgaggct ggggattagg ttcagaaata tttgataagc attagtatag
     3241 tgcgcgaggt catagctttc aacaagttcg actgtcgccc gataaagaag atgaaattgc
     3301 cgagtgaggg gaagttcgct ctcaatgatt tcgtatctgg tcgtcctcac tcctaatggt
     3361 atgcctaatg ataggagaaa atgcatgccg tcggcgtatt catcgaggag aacctcgcga
     3421 ggagatggtc ctttgtttga ccagtattta aagcaccgcg tctcgttggc gagctcaccg
     3481 atttcgacaa tcagagccat caggcggtcg tctgtggtct tctcgtatga ggttttatga
     3541 ttactcgcta tgcgagcgtc taactcacct tgtaacctat acagctcttt tagttcaatc
     3601 atctggctca tatttttaac ttccttaaat gccagatatc gcggacgtat tgttcaatcg
     3661 tccggtcgct ggtgaaaaat ccggaattag cgatgttgat gatttgcgag cgggcccagt
     3721 ttcgtcgatc gagatagagt ttttcgattt tttcctgagc ttcgacatag gcggcgaagt
     3781 ccttcagaat gaagtaggtg tcgttacgat gcataacctc atcaaaaatg agttgatact
     3841 tctcgtcttt gccgaatgtc cacggaccgc taaacaggga atcgatgact tttttaatgc
     3901 gctcatcaga gttgtagatg tcccacggat tgtaatcgcc gcgctcatag tggccttcga
     3961 cgtcctcggc ctttaaacca aagatgacgc agttatccaa accgacaaag tcggcgattt
     4021 cgacattggc gccgtcgagg gtgccgagcg taatcgctcc gttcatcatg aatttcatgt
     4081 tgccggtgcc gctagcttcc ttgccggcgg ttgagatctg ctcgctgata tcgctggcgg
     4141 gaatgatttt ttccgctgac gagacgccgt agttttcaac gaataaaact ttaatgaatt
     4201 ttgagacgtg cggatcgcgg tcgatgatgt cggccaccgc caaaatgagc tcgatgattt
     4261 tcttggcata atcatagctc ggagcggcct tggccccgaa gacaaaagtc cgcggaaaga
     4321 tggtaaaaga cggatcatcc cttagacgct gatagagata gataatgtgg aaaatattca
     4381 ttaactgccg cttataagcg tgcagacgtt tgatttggac atcgaaaatc gaatcgacat
     4441 ttaaatcgac attctgcttt ttcttcataa atgaagcaaa ggcgattttg ttgtcgtgct
     4501 tgatgcgcaa aatcgcatct tgtacttttt tgtcgtcttt atattgctcg aagtctttga
     4561 tgaggctaaa gtcttttatc cagccctcgc cgatcttact cgtgatcagc gcggccagtt
     4621 cgggattggc atacatcagc cagcggcgat gggtgacgcc gtttgtttta ttgttgaact
     4681 tctgaggata taattcgtag aaatcgcgaa aggtatcgcg ctttaaaatc tcggtgtgca
     4741 gtttggcgac gccgttgacc gagtaagagg catggatggc tagatttgtc atgtgaattt
     4801 gcccgtcttt gataatttgc atcgcgtaac gcttgccgta atcgatgttt ttctcggcca
     4861 tttctagatt gaaacggcgg ctgatttcct cgatgatcat gaagcagcgc ggcaatagtc
     4921 tttgcacgta ttgaatcggc catttttcga gggcttcggc catgacggtg tgattcgtgt
     4981 aggcaaacga tttctgcacc tggcaccagg cctcttccca ttcgtattcg tattcgtcga
     5041 tcaaaagtct catcatctcc ggaatggcta aaatcggatg ggtgtcgttg agctgaaaga
     5101 cgtggtaatc agcaaaatta tcgagggtac cgaaacggcg gaaatggccg cgcattgcgc
     5161 tttgaaggcc ggcgctgacc aagaaatact gctgccttaa ccgtaaaatt cggccctgct
     5221 cggtcgaatc atcgggatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     5341 gaatgcagtt gccgtgcccg gcatagccaa gcgacgccag cgaatccatg aagcaggcgg
     5401 cgaggcggcc gaggcccccg ttgcccaggc cggcatcatg ctcttcgtct tcgagttcat
     5461 tgatatcgat tccgagttct ttaagacctt gttcggcaat ctcgtagatg ccgaggttca
     5521 tcatgttgct gtgcatcaat ttgccaatta aaaattccat cgaaaaataa atgagttgct
     5581 tttctttatt ttttaagacg tactcgcgcg tttgccgcca gtagttgccg gcataatcgc
     5641 ggactaattc gccgaggatg tcgtagcgtt cgctgatgtg ggaatccttg acgttcttgc
     5701 cgtatttttc ggataagcgc ttggaaaact catccttgaa ttctgctttg tttttaaaca
     5761 tatttctcct ttatttctta ttcttccgat gcttgaaaat catcgctcca aaggaagcta
     5821 ttttaatatt gatgtgatgc gggcggttaa agggcccaaa aggcgcgctc ggcaccggta
     5881 atccattata ctggttcgcg ccgccatata catctttatc gctgttaaag atttcttcat
     5941 aggtgccttc ttcgttgaca cccagttcgt aattatcaaa gaagttgggg gtcatattga
     6001 agacgaaaat gaggcgcgaa tcatcgacaa aacgctcgaa ggcgtagatt gactgatcag
     6061 cgttatcagc catcagccac aggaaatgct tgggattgta ttcctcgaat ttcatggctt
     6121 tttcataaga gtaaataaga tttaaatcgc gaatcagccg cgtgatggaa tcgtgtttgg
     6181 gaaatgttcg aagattccac ggcagcgact tgttttcatt ccactcgtca aatgaggcca
     6241 gttcattgcc catgaaattg agttttttgc cgggatgtcc aaactgatag gtgtagagat
     6301 tgcgcagcaa ggcaaatttc tgttcataat cgccccacag cttattgata atcgtgccct
     6361 taccgtgaac gacttcgtcg tgggagaggg gcaaaagaaa attttcgctg aagaagtagg
     6421 ccatcgagaa agttaatttg tgatgttcat actttttata gatggggtct ttggcatagt
     6481 acttcaaggt atcgttcatc cagcccaaat cccatttgta gtcaaagccg aggccgccga
     6541 actccagcgg cttggtcaca ccctgatagt cgctgctgtc ttcggcgatc gtcatcaagc
     6601 tggaatgctc gccgtgaagc aaggtgttca tccgcttcaa aaattcgatg ccgccggcgt
     6661 tttcgccctt atctttattg ccgtcataaa aaatgacgtt gctgacggca tcgatgcgga
     6721 gaccatcaaa atggaaatag ctcgcgaaat aattcatcgc cgagatgaga aaagaacgga
     6781 ccgggtcttt gccgaggtca aagaggaggc ttccccactg cgaataacga tgtttaatat
     6841 cggtgtattc atatagataa gcaccgtcga aatccgccag agcaaattcg tctttggcaa
     6901 aatggacggg ggcgaagtcg aggatgacgc cgaggccggc ctgatgcatg cgatcgacga
     6961 atgacatcag ctgtttggga ttgccgtagc gcgaatcgac ggaataaaag ccggttccct
     7021 gataacccca ggaaccatcg aatggatatt gggtaatcgg catgatttcg acatgagtgt
     7081 agcccaattc tttaacgtaa ggaattaaat aatcggcgat ttcttcgtag ctcaggttgc
     7141 gattatcgac tttacccttc cacgagccga ggtgaatttc atagattgac atcggatcat
     7201 caaaagagcg gttgcggctt ttcatgtatg acgaatcgtg ccagatgaag ttttcgatat
     7261 cgaagaggcg cgagcaagtt gcgggccggt attcgctata aaaagcatag ggatcggctt
     7321 tatcgacata ttggcctttg gcgttccgaa aatgatattt atatgattca tactctttta
     7381 acccgggaat aaaaacttcc cagacgccgg ccaaatcgac tttctgcatt ttatgagcgc
     7441 cgacatccca gttgttaaac gagccgatga cgctgacgtc ctcagctaga ggagcataaa
     7501 gacgaaacat gacgccgcgc tcttttttcc cctcggtttc tgatgcaata aaatgagccc
     7561 cgaaatactc gtaagccctg gtcgtgttgc ccgttaagaa gtcgtgaagt agtccataag
     7621 ccattgtttg tgtcccgctt tctaagataa ttataa
//
LOCUS       DAOSSL010000037         7054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_9913_length_7054_cov_18.170739, whole genome shotgun sequence.
ACCESSION   DAOSSL010000037 DAOSSL010000000
VERSION     DAOSSL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9913_length_7054_cov_18.170739"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLH17_04055"
     CDS             complement(<1..494)
                     /locus_tag="PLH17_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOR20976.1"
                     /translation="MEMMELMKSRHSVRRYLEKPIAEDLRTAINNYIDKTRQESGLNI
                     QVCYDEPRAFKTLLAHYGSFRNAVNYIAFVGKKEEQTKVGYYGEAIVLKLQELGINTC
                     WVAVSYSKRKTALKINKGETILCVISFGYGETQGTMHRSKKAADVSEVIGEKPSWFDA
                     GVEAA"
     gene            complement(506..1009)
                     /locus_tag="PLH17_04060"
     CDS             complement(506..1009)
                     /locus_tag="PLH17_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOR20977.1"
                     /translation="MNGLRLARLSDLAQIMDVVKDAREFLKAQNSGQWQDGTPSIATI
                     AEDSMNNRFFVWEEEGVIIGIIALLDHDNDYENLKSGQWRFPAPYLVIHRFAVRSDYH
                     AQGIATKILQAVELIAGERNIHTIRVDTHELNIPMIGLLEKNGFAKCGEVLIEGIKPR
                     ITFDKKI"
     gene            complement(1022..2029)
                     /locus_tag="PLH17_04065"
     CDS             complement(1022..2029)
                     /locus_tag="PLH17_04065"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOR20978.1"
                     /translation="MNQKEIKQKTVLLNHQGQLNIAGYAKTMQFSYNRGEIRSFPLKL
                     KEWNFYQFIKGEDIVQLTIGHVSYTCSVTATLLNIKTGVKHQIGKMKWFFAPELDVNP
                     EKESYNEFKSDDFLMTFKVTDKERILTFKGKNERYRNVDILIVAENDIHNEKMVIATP
                     FKKKKQFYLNYKENYYHAKGHVIFDGINIDLEGATGLLDWGRGIWPYRHEWFWGSASA
                     FIDGVPFGLNIGWGFGDLSNATENMYFYDKLAYKVGVLKVERDYKNYLAPWHLKDEEG
                     TIELFFEPAYDNYTENKFVIVDTHCDQVYGYFRGTIMTSEGEKRFSQVLGFIEHAVNR
                     W"
     gene            complement(1989..3470)
                     /locus_tag="PLH17_04070"
     CDS             complement(1989..3470)
                     /locus_tag="PLH17_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002689121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20979.1"
                     /translation="MKPTVDKKEKKYYGLGSFRFWFIIMAMGLAGHLCWNMENQWFNT
                     FVYAKIGGDVTIVTWMVIVSATVTTLSTFIFGTLSDRLGKRKRFVWIGYMMWGVSTIL
                     FGTTEFAKSSNISALVAMAGFLVVFTDAIMSFFGSMAFDAGYNTWVNDYTTPKNKGQV
                     GAALAALPVIGTVVGTVLGGALVNIGNPTVGTPNYDPSQDNYQLLFWALGIAVILIAV
                     ASIFLMKDAPTTKPNKDGSYFKQLFDVFNFKKLKGNPNNKEMLLANLVACFFFIPFNF
                     YFVHMGNWLIYDIGFTAGDMGLIQGISLLLAVLVTIPFAKLINKNRIPLVALVAVLVN
                     AVGLLIMFFFIKGPESVDTSSFLAAKNLPLLLAVFLVGTGYVLITQTCMIWVRGLFPE
                     ENRGQFEGIRSLFFTLVPMLIGTLVGNIIIKNTSHEIQYDLYDNPIDIPQENLFLFAG
                     LLVLLTVIPLGIAWKVYNKRMKAEALSKLKDESEGNKAEDRPA"
     gene            complement(3479..4801)
                     /locus_tag="PLH17_04075"
     CDS             complement(3479..4801)
                     /locus_tag="PLH17_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOR20980.1"
                     /translation="MSNYDVVIVGACTAGTYFSNLLAKQGLKVLVIDKDEEENLSRRL
                     DIFHFTHQSFQDYGLEEAKSGDEEFVRNFDLCYSKSALDNYLKKSFLKVAVMHLPLFI
                     KRLRKTALANGVTFRFGVSYDHLLYDEQKRIKGIAAKSGEEIFARLVVDASGIPSVVR
                     RSISDPFIEDFEIGPRDKFYVLLKYVELSDAKDKVELSTSWPYYKGWIAPQHSANGAI
                     VGVGANLSYDYARKCMAKFERSIPLPNYKLQYEELGCTPYRRPPFSFVTDGFLVIGDA
                     ACLTKPMNGEGITSAWVQCTPAAEVVAHALRDGKYPTKEALWKINTLYQRGEGAEFAN
                     LRATLIGAVDMSPKDNDYMFKESIVFKSDDEEVKGSVTGKLIKGVLTGQFSLKALRSL
                     MKSSSCGQKLEKHYKNYPDNPKDYPAWKKKADKLWSKAGTMADNIKDA"
     gene            complement(4971..6191)
                     /locus_tag="PLH17_04080"
     CDS             complement(4971..6191)
                     /locus_tag="PLH17_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR20981.1"
                     /translation="MTGNIKKQAILFLVLLGVISLLSDFTHEGARSIYGPFLGLIGAS
                     AFVVSFTSGLGEFIGQALRIATGVIADKSKKYWGMMFLGYAVNLLAIPLLAFVDASIW
                     QVAIVLILLERVGKAIRAPAKSALVSFTTPHLGAGKSFAIQEVLDQIGAFLGPLFAFA
                     ILSFNQGSELNGYQMTFGFLGIFAVATLVILIVSWKKYPNPEAFDNRTAFSGFKGNKA
                     FIWYMLAVSFLALGFIDYPLMAFHMEATNAIGITYIPLLYSLAMGIDALAALLFGYLF
                     DKYGVRALQISTFIAAFSVPVFFLFNGLWAIIVGLIFWGIGMGAQESILKAVISSIVS
                     KEKRATAYGIFYSVFGLAWFLGSTLIGIVYDFSILAIVIYSFVMEVGAVLLLFVFSRT
                     QKKTQIQKETPIQS"
     gene            complement(6300..>7054)
                     /locus_tag="PLH17_04085"
     CDS             complement(6300..>7054)
                     /locus_tag="PLH17_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOR20982.1"
                     /translation="TVADFDPILMLDSFDSTNPRDYIAGFPLHPHRGIETISYIYRGK
                     MVHKDSLGNEDMISDGEVQWMTAGSGIMHEEKLPASERMLGVQLWLNLSAKDKMVAPA
                     YQSIKADQIKDVILPHGKLRVLAGSYRGQTSYQSKYQPLDYYDIHLNPHASLTLDTDP
                     RRSIMLFTLVGSIIVGGVAIKEKTAVKLSEGDTLTIQMGNESAQILFVSSLALNEPVA
                     WGGPIVMNSKEELYRAFQDLDEGTFLRQSIKY"
BASE COUNT         1865 a         1642 c         1690 g         1857 t
ORIGIN      
        1 gcggcttcga cgccggcatc aaaccacgat ggcttctcac cgatgacttc cgagacatca
       61 gcagctttct tcgaacggtg catagtccct tgcgtttcac catagccaaa cgaaatgacg
      121 cataagattg tttcgccttt gtttatctta agtgccgtct ttcttttgct ataggagacg
      181 gcgacccagc atgtattaat gcctaattcc tggagtttta aaacgatggc ttcaccataa
      241 tagccaacct ttgtctgctc ttcttttttg ccgacaaaag cgatgtaatt gacggcgttg
      301 cgaaagctac cgtaatgagc caaaagagtt ttaaaagcgc gcggctcatc atagcaaacc
      361 tggatgttca atccgctctc ttgccgagtc ttatcaatat aattattgat agcagttctt
      421 aaatcttcag cgatcggctt ttctaggtag cggcgcaccg agtggcggct tttcatcaac
      481 tccatcattt ccatcttctg ttctcctaaa tctttttatc aaaggtgatg cgcggtttga
      541 ttccttcaat cagcacttca ccgcacttag cgaatccgtt cttctccaat aagccaatca
      601 tcggaatgtt taactcatgc gtatcgacgc ggatggtatg tatgtttctt tcgccggcaa
      661 tcagctcgac cgcctggagt atcttcgtgg caattccctg ggcgtgatag tcgcttctga
      721 ccgcaaagcg atgaatgact aaatacgggg cgggaaaacg ccactgcccg gatttaaggt
      781 tttcataatc gttgtcgtga tctaaaaggg cgatgatgcc gataatcacg ccttcttctt
      841 cccagacaaa aaagcggttg ttcatgctat cttcggcaat cgtcgcaatc gagggcgtgc
      901 cgtcttgcca ctgccccgag ttctgcgctt ttaaaaactc tcgggcgtct ttaacgacgt
      961 ccatgatttg ggctaaatca gagaggcggg caaggcgaag tccattcatt atatgaatat
     1021 tttaccaccg attgacggcg tgttcgataa agccgaggac ttgcgaaaag cgcttttctc
     1081 cttcgcttgt cataatcgtg ccgcgaaagt atccgtatac ttgatcgcag tgcgtgtcga
     1141 cgatgacaaa tttgttttcg gtgtagttat cgtaggcagg ttcgaagaat agctcaatcg
     1201 ttccttcttc gtctttgagg tgccagggcg caagatagtt tttataatct ctttcgactt
     1261 tcagaacccc aactttgtaa gccagcttat catagaaata catattttcc gtcgcgtttg
     1321 ataagtcgcc gaagccccag ccgatgttga gtccaaacgg aacgccatcg atgaaggcgc
     1381 tggcgctgcc ccaaaaccac tcgtggcgat acggccagat gccgcgcccc cagtcgagga
     1441 ggccagtcgc gccttctaaa tcaatgttaa taccatcaaa gatgacgtgc cctttagcgt
     1501 gataataatt ttctttgtag tttaaataaa attgcttctt ctttttaaaa ggtgtggcga
     1561 tgaccatctt ctcgttatga atatcgtttt cggcgacgat taaaatatcg acattccgat
     1621 acctttcgtt tttgccttta aaggtcagaa tgcgctcctt atccgtgact ttaaaagtca
     1681 ttaagaagtc atcgctttta aactcgttat aactttcttt ttccggattg acatccagtt
     1741 cgggagcaaa gaaccatttc atcttgccga tttggtgttt tactccggtc ttaatattta
     1801 aaagcgtggc cgtcaccgaa cacgtatagg aaacatggcc gatcgtcagc tggacgatat
     1861 cctcgccttt aatgaactgg tagaagttcc attccttcaa tttaagcgga aaagagcgaa
     1921 tttccccgcg gttatacgaa aactgcatcg tttttgcata gccggcgatg tttagttgac
     1981 cctgatgatt aagcaggacg gtcttctgct ttatttcctt ctgattcatc ttttaattta
     2041 ctcagtgcct cggctttcat ccgcttgtta tacaccttcc aggcaatgcc caaaggtatg
     2101 acggtgagca agacgagtaa ccccgcaaaa aggaataaat tttcctgcgg tatatcgatt
     2161 gggttgtcgt ataaatcgta ttgtatctcg tgcgaggtat ttttgatgat gatgttaccc
     2221 actaatgttc cgatcagcat cggcacaagc gtgaagaata aggaacgaat gccttcaaac
     2281 tggccgcggt tttcttcggg gaacaagccg cggacccaaa tcatgcatgt ctgggtaatt
     2341 aagacataac cagttcccac caggaagacg gcgaggagaa gagggagatt tttggccgcc
     2401 agaaaagacg aagtgtcaac gctttccggc cctttgataa agaagaacat aattaagagg
     2461 ccgaccgcgt tgacgagcac cgcgaccagg gcgacaagcg gtatgcggtt tttattgatg
     2521 agtttagcaa agggaatcgt gaccaaaaca gccaaaagca aggatatacc ttgaatcaga
     2581 cccatatctc cagccgtgaa gccgatatca tagataagcc agttgcccat atggacaaaa
     2641 tagaaattaa aggggataaa gaagaagcag gccaccaaat tagccaggag catctcttta
     2701 ttgttcggat tacctttaag tttcttaaaa ttgaaaacgt caaacaattg tttaaagtag
     2761 gaaccatctt tgtttggttt ggttgtcggc gcgtctttca ttagaaagat ggaagcgacg
     2821 gcaatcaaaa taacggcgat gccgagggcc caaaacaata attggtaatt atcttggctt
     2881 ggatcatagt ttggggttcc aaccgttgga ttaccaatat tgactaaggc tccaccgaga
     2941 acagtgccga caacggtgcc gataactggc aaagcggcaa gagctgcgcc gacttggcct
     3001 ttattcttcg gcgtcgtgta atcgttgacc caggtattat atccggcgtc aaacgccatt
     3061 gaaccaaaga aactcattat cgcatccgtg aagacaacta agaagccggc catggcgacc
     3121 aaagcgctga tattggagct tttggcgaat tcggtcgtgc cgaagagaat tgtcgacacg
     3181 ccccacatca tatagccaat ccaaacgaat cttttccgct ttcctaatct atctgagagg
     3241 gtgccaaaga tgaaggtcga aagagtcgtg accgtcgcgc tgacgatgac catccaggtg
     3301 acgatggtga catcgccgcc gatcttggcg tagacaaagg tgttgaacca ctggttttcc
     3361 atgttccagc ataaatgccc ggccaaaccc atggccatga taataaacca gaagcgaaaa
     3421 ctacccagac cataatactt cttttctttc ttatcgactg ttggtttcat aatgcctcct
     3481 atgcatcctt gatgttatcc gccatcgtcc cggctttgct ccaaagttta tcggctttct
     3541 ttttccacgc cgggtaatct ttcggattat cgggataatt cttgtaatgt ttttccagct
     3601 tttgcccgca gctggacgat ttcattaacg agcgcaacgc cttgagcgag aactgcccgg
     3661 tcaagacacc tttaatgagt ttgccggtca cgctgccttt gacttcttcg tcatcagatt
     3721 taaagacaat actttcttta aacatatagt cgttatcttt tggtgacatg tcgacggcgc
     3781 cgattaaggt ggcgcgcaaa ttggcgaact ccgcgccttc acctctttga taaagagtgt
     3841 taattttcca aagggcttct ttggttgggt attttccgtc tcggagggcg tgggcgacga
     3901 cttccgccgc cggcgtgcac tggacccagg ccgaggtgat gccctcgccg ttcatcggtt
     3961 tcgtcaggca ggcggcatcg ccgatgacta agaaaccatc ggtgacaaag ctaaacggcg
     4021 ggcgccggta aggcgtgcaa ccaagctctt catattggag tttatagttc ggaaggggta
     4081 tgcttctttc aaatttggcc atgcacttgc gggcgtagtc ataagagaga ttggcgccga
     4141 cgccgacaat cgctccgttc gcggaatgct gaggcgcgat ccagccttta taatagggcc
     4201 agctggtgct taattcgact ttatctttgg cgtctgataa ctcgacgtat ttaagcaaaa
     4261 cataaaattt gtcgcggggg ccgatttcaa agtcttcgat aaagggatcg ctaatcgaac
     4321 ggcgcacgac cgaaggaatg cctgaggcat cgacgacgag acgggcgaag atttcttctc
     4381 cgcttttagc ggcgatacct ttaattcttt tctgttcgtc gtaaagtaga tgatcatagc
     4441 tgactccaaa acggaaggtg acgccgttag caagcgccgt ctttcttaac cgcttaataa
     4501 aaagcggcag gtgcatgacc gccactttta aaaagctttt ctttaaataa ttatcaagtg
     4561 ctgattttga atagcagaga tcgaagttgc ggacgaattc ttcatcgccg ctcttcgcct
     4621 cttcgaggcc gtaatcttgg aaagattgat gcgtgaaatg gaagatgtcg agcctcctgg
     4681 aaagattttc ttcctcgtct ttatcgatga cgaggacttt taaaccttgt ttggcgagga
     4741 gattagaaaa gtatgtgccc gctgtgcagg ctccgacaat tacaacatca taattagaca
     4801 tgctaatgcc ccctgttaaa tcagttctgc cattattcta aactttaact gcgcgcgttt
     4861 caaagacaca atcaagacag aatcttcatt catcaaagcg aatccatcta tatggtgtaa
     4921 aacatttcca aacttatata tgaaatttgt ttcacatttt tgacattaga ttacgactgg
     4981 attggcgttt ctttttgaat ctgcgttttc ttttgagtgc gggagaagac gaatagcaaa
     5041 agaaccgcgc cgacttccat gacaaaggaa tagatgacga tggccaggat tgaaaaatca
     5101 tagacgatgc cgattagggt cgaacctaaa aaccaggcta aaccaaatac cgaataaaag
     5161 atgccgtagg ccgtcgcgcg cttttctttg gagacgatcg acgagatgac ggcttttaaa
     5221 atcgactcct gcgcacccat gccgataccc cagaagatga ggccgacgat aattgcccat
     5281 aaaccgttaa aaagaaagaa gactggcacg gaaaaggcgg cgataaaggt gctgatctgt
     5341 agcgctctaa cgccgtattt gtcaaagagg tagccgaaga gcagggcggc gagagcgtcg
     5401 atgcccatcg ccagagaata aagaagggga atgtaagtga ttccgatggc gttggtcgct
     5461 tccatatgga aagccatcaa cggataatcg ataaaaccta aggccagaaa gctgaccgcc
     5521 agcatgtacc agataaaagc tttattgcct ttaaaaccgc tgaaagcggt gcggttatcg
     5581 aaagcttcgg gattcggata ctttttccac gagacgatca ggataactaa agtggcgacg
     5641 gcaaaaatgc cgagaaagcc aaacgtcatc tggtagccat taagttcgct gccttgatta
     5701 aaactcagaa tggcaaaggc aaacaagggt cccaaaaagg ctccgatctg atcgagcact
     5761 tcctgaatgg caaaggattt gccagcacct aaatggggtg tggtaaagga aaccagcgcg
     5821 ctcttagcgg gagcgcgtat ggctttgcca actctttcta ataaaattag aacaatagcc
     5881 acttgccata tcgaggcatc gacaaaagcc aagaggggga tggccaagag atttacggca
     5941 tagcctaaaa acatcatgcc ccagtatttt ttgctcttgt cggcgatgac gccggtcgcg
     6001 atgcgcaggg cctggccgat aaactcgccg aggccggaag taaacgaaac gacaaaagcc
     6061 gaagcgccga tcagcccgag aaaagggcca tagatgctgc gcgccccttc gtgggtaaaa
     6121 tccgataata agctgatgac gccgaggagg acgaggaaga gaatcgcctg ttttttaatg
     6181 ttgccggtca taattaatcc tcagtgaaga tagtatttat attttatggc ggcggccggc
     6241 ttctttaaat aaaaaaacac tcgcctgaga caagaaaatt gtcgggcgag cgtcattgac
     6301 tagtatttaa tgctttgcct gagaaatgtg ccttcatcca aatcctgaaa ggcgcggtag
     6361 agttcttctt tgctgttcat gacaatcggt ccgccccagg cgacgggttc atttaaagcc
     6421 agcgagctga cgaagaggat ctgggcgctc tcatttccca tctgaatcgt taaagtatcg
     6481 ccttcgctga gcttcaccgc cgttttctct ttgatagcga cgcctccgac gatgattgag
     6541 ccgacgagag taaagagcat gattgagcgg cgcggatcgg tgtctaaggt cagcgaagca
     6601 tgaggattga gatgaatatc ataataatcg agaggctgat atttgctttg ataactcgtc
     6661 tgtccccgat aagaaccggc gaggacgcga agtttgccat gaggcaagat aacatcttta
     6721 atctgatcgg ctttgatgct ctgataggcg ggagcgacca tcttgtcctt ggccgatagg
     6781 ttaagccaga gttggacgcc cagcattctt tccgaggctg ggagtttttc ctcgtgcatg
     6841 atgccggagc cggccgtcat ccactgcact tcgccgtcgc taatcatgtc ttcgtttccg
     6901 aggctgtctt tatggaccat cttgccgcgg tagatatagg agatcgtttc gatgccgcga
     6961 tggggatgaa gtggaaagcc cgcgatataa tcgcgcggat tggtgctatc aaacgaatca
     7021 agcattaaaa tcggatcaaa atcggcgacc gttc
//
LOCUS       DAOSSL010000038         6887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_10191_length_6887_cov_16.537032, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000038 DAOSSL010000000
VERSION     DAOSSL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10191_length_6887_cov_16.537032"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLH17_04090"
     rRNA            complement(<1..343)
                     /locus_tag="PLH17_04090"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(682..1062)
                     /locus_tag="PLH17_04095"
     CDS             complement(682..1062)
                     /locus_tag="PLH17_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOR20983.1"
                     /translation="MSKFFMCPICGNFVESIVDSGVPMICCGQEMEEVEADTQENVAL
                     EKHIPEVKVEGHKVIVKVGSVTHPMLPEHFIQWIHLETNLGIRRTKLEPGQEPKATFC
                     LVEGEVAVAAYEYCNLHGLWKKAV"
     gene            complement(1140..1841)
                     /locus_tag="PLH17_04100"
     CDS             complement(1140..1841)
                     /locus_tag="PLH17_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HOR20984.1"
                     /translation="MNNYRTYEPGVQSATGSKFLGTVFGYAAIGFLVTAAVALLTGYL
                     FTSVIPIDVASDTYLGLIIGASILQIVLIFWITFGVFRRGGNLIIPFALYTITMGILL
                     STLTLTMDWYLIGMTFGLTCLVFGAMAAVGYFSKVNLSGLLIVGMGLLIGSIFLSLIN
                     WFIGSDQLGWIVTFASFGAIMLITAFDVWRIRRISESGQASKNLAMYCAFNIYIDFIY
                     MFMRIAMILSRVSRR"
     gene            complement(1854..>2312)
                     /locus_tag="PLH17_04105"
     CDS             complement(1854..>2312)
                     /locus_tag="PLH17_04105"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOR20985.1"
                     /translation="RTYDNETSERCNLKIKALLANEMTPVYCVGETLAQFEKNETKKV
                     IKEQISAGFSEISSENAAKVVVAYEPVWSIGTGRNASSEIAQDVCSYIRGLLREKYGE
                     VAEQIRILYGGSVKPENIKTYLSTADIDGALVGGASLSIESFKELINNMI"
     assembly_gap    2313..2412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2418..3638)
                     /locus_tag="PLH17_04110"
     CDS             complement(2418..3638)
                     /locus_tag="PLH17_04110"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOR20986.1"
                     /translation="MIMLTIDQLSDLKGKRVLVRVDFNVPLKDGEIRDDNRIVQAAPT
                     IKMLISHGARVILLSHLGKIKHKEAPEVVEAQKKKNSLRPVAARLGEILRVPVAFCPD
                     TTGEKAKFFMGVVEPGEVVLMENTRYEKGEEKNDPELAKKWAENIDIFVMDAFGSAHR
                     AHASTYGVPEILKAQGKPVAIGYLVEKEVRNLTRCVEVKDGDRPYIAILGGFKVSDKI
                     KVIDSLLKKCDKILIGGAMAYTFAKALGRTIGKSPCEDDQLEYAKKCFEEANGRILLP
                     LDSIATDAFEGWTQKIVTDDANIPGEMEGMDIGPKTAALFQQEISKAKMVFWNGPMGV
                     FEQKDFAGGTIAVCQAIAKLKSAFTVCGGGDSAAAVKEFGFSDAFSHVSTGGGASLEM
                     IENDGHLPGIDILK"
     gene            complement(3691..4728)
                     /gene="gap"
                     /locus_tag="PLH17_04115"
     CDS             complement(3691..4728)
                     /gene="gap"
                     /locus_tag="PLH17_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005967316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOR20987.1"
                     /translation="MSIKVAINGFGRIGRLAFRRAYETGEFEVVAINDLADAASLAYL
                     LKYDTAHKRFGVDDITVDVEKNAIVYKGKAIPVIGQPDPKLIKWSDYGAELVLECTGR
                     FKGKADAAVHITEGGAKGVVISAPADKETPTFVYGVNEGKLTKDMTVISGASCTTNCL
                     APVAKVLVDKFGIKGGYMTTVHAYTNDQTTLDLVKKGNSRRGRAAAANIVPTTTGAAK
                     AINLVIPELKGRLQGGALRVPVIDGSLVDLSLLLNKDVTVEEINAAMKEAADTYLKDV
                     LAYTEDEIVSSDIIGATAGSIFDATQTAVFTNPDGQQFVKVVAWYDNEYSYTCQYLRI
                     AKEMAKVLGLK"
     gene            complement(4856..5083)
                     /locus_tag="PLH17_04120"
     CDS             complement(4856..5083)
                     /locus_tag="PLH17_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOR20988.1"
                     /translation="MKTNLQLGQKIAALRRARGWNQEEMAFRASLNRNYLSDLENGRR
                     NPSLKVLEKIAFAFEINLETLFRGITSFPYV"
     gene            complement(5073..6038)
                     /gene="whiA"
                     /locus_tag="PLH17_04125"
     CDS             complement(5073..6038)
                     /gene="whiA"
                     /locus_tag="PLH17_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOR20989.1"
                     /translation="MRPTLSFTRKVKEEIVTKPFTEERLRALVSAFIKVNGRMEIKNR
                     CVAILLQTENAKIAKFIYQIIQQIYGINIRFSYEKTMKFKRRITYNIIIDQEAEYIMG
                     DLEISFLEGKIAKNIVFNDDMIGGYLAGAFLASGSVNSPRSSNYHFEIALNDENYAKW
                     FLKLIAKYKGGSFSPKTIKRREQIVIYLKKSDQIADFLILMGATASSLEFENIRIDRE
                     FANIGNRLQNLDTANYEKTLTSAARQLDDLRTIDKILGVKNLANEKQRLLAEIRLQNE
                     DATMEELAAMLSEKMDKVVTKSNVNHIFRALRVLAKRYRGDDDEN"
     gene            complement(6035..>6887)
                     /gene="rapZ"
                     /locus_tag="PLH17_04130"
     CDS             complement(6035..>6887)
                     /gene="rapZ"
                     /locus_tag="PLH17_04130"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOR20990.1"
                     /translation="FMIDLIILTGVSGAGKSTAVRTFEEMDYYIVENVPFSVIPKLYK
                     AFEDDPVRYRRTVVVVAIDEALKAAQAARKNTNFRVTFLALDASEAELLTRFRLTRHI
                     HPAQAHGLSLADSLERDRKIMDVLKKDVDMYVDTTGKTATELRKFIFTNIQGMESGNL
                     TISFVSFGFKHGVPNDVEMIFDTRIVPNPYWISELKDMTGLDEPVKKFVLEDKEAKEL
                     IKNIEKYLDYYLAKVRAEDRNFFVVGIGCSGGQHRSVVIAQYLASKYAANYRTLCLHR
                     DIGKFKK"
BASE COUNT         1691 a         1576 c         1680 g         1840 t
ORIGIN      
        1 cccgtaggag tctggaccgt gtctcagttc cagtgtggcc gatcaccctc tcaggtcggc
       61 tacgcatcct cgtcttggtg ggctgttatc tcaccaacta actaatgcgc cgcaggtcca
      121 tcaatcagcg aggctttaaa ggcccctttt atgctccgaa gatgccttcg aagcacgaat
      181 tcggtattag ccaaagtttc cctcggttat ccccaactga aaggcaggtt acctacgtgt
      241 tactcacccg ttcgccacta aggtattgct accttcgttc gacttgcatg tattaggcac
      301 gccgccagcg ttcatcctga gccaggatca aactctcaaa agtttttatc tagttcaaaa
      361 ttattattct ggtttatttg tatattatta aaaaattgac gttaatgttg tttgtacatc
      421 tgttcagttt tcaaagatca gctgcatgct atacgctagt ataggccctc tcgggtcaaa
      481 tcgcatgctt agataatgta tcattcatac ttataaagtg tcaagaacaa aaacttattt
      541 tttgcgatta tttacttagt aaggaggcct acgcgacaat cctgacgcta tctacgggaa
      601 ataacaaact gaccagcgct ctttttttat ataaaaagac cagcgcatga cgccggcctt
      661 tgtttgacga ttgataagag tttagacagc ctttttccaa agaccgtgta aattgcaata
      721 ttcgtaagcg gcaaccgcta cttcgccttc gacgaggcag aaggtggctt tgggctcttg
      781 accgggctcc aatttcgtcc ggcggatgcc gagattggtc tcgagatgaa tccactgaat
      841 gaagtgctca gggagcatcg gatgagtgac ggaaccgact ttgacgatga ctttgtgacc
      901 ttcgactttg acttcgggga tgtgtttctc aagggcgacg ttttcctgag tgtcggcctc
      961 gacttcttcc atctcctgtc cgcagcaaat catcggcacg ccgctatcga caatcgactc
     1021 aacgaaatta ccacagatgg ggcacataaa gaattttgac attttgttac ctctcttttc
     1081 ctaaaatata ttttaccttg ttttaaaagg tgtttgaaca acaatagtaa attttttgtc
     1141 tagcggcggc tcacgcgcga aagaatcatg gctatgcgca tgaacatgta gataaagtca
     1201 atgtagatat taaaagcgca atacatggcg agatttttgc tcgcctgacc gctctcgctg
     1261 atgcggcgaa tgcgccacac atcaaaggcg gtgatgagca tgatggcccc gaacgaggca
     1321 aatgtgacaa tccagccgag ttgatcgctt ccgataaacc agttaatcaa agataaaaag
     1381 attgaaccga ttaaaaggcc catgccgacg attaataaac ctgagaggtt gacctttgaa
     1441 aaatagccga ccgcggccat cgcgccaaag acgaggcacg tcaggccaaa agtcatgccg
     1501 atgagatacc aatccatcgt cagtgttaac gtcgagagta aaatgcccat cgtaattgtg
     1561 tatagggcaa agggaataat cagattcccg ccgcggcgaa agacgccaaa agtaatccag
     1621 aagatgagga cgatttgtaa aatcgaagcc ccgatgataa gtcctagata agtgtcgctg
     1681 gcgacatcga tgggaatgac gctcgtaaac agatagccgg tcagcaaggc gacggctgcc
     1741 gtaactaaga agccgatggc ggcatagccg aacaccgtac cgagaaattt acttcccgtc
     1801 gcggattgca ctcctggttc atatgtccgg taattattca atgtcagtac ctcctaaatc
     1861 atgttgttaa tcagttcttt aaacgattca atgctgagcg aggcgccgcc gactagggcg
     1921 ccatcgatat cggccgtcga aagatatgtt ttaatatttt ccggtttgac ggaaccgccg
     1981 tagagaatgc gaatctgttc agcgacttcg ccatactttt cgcgcagcaa tccgcgaatg
     2041 taagagcaga cgtcctgagc gatttctgaa gaagcatttc ttcccgtgcc aatcgaccaa
     2101 acgggctcat aggcgacgac gactttggcg gcattttcag agcttatttc actaaaacca
     2161 gctgatattt gctcttttat tacttttttt gtttcgtttt tttcaaattg agccagcgtt
     2221 tcgccgacgc agtagacggg tgtcatctcg ttggcgagaa gggctttgat tttgaggttg
     2281 cagcgctcgc ttgtctcatt atcataagtg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnattaccta ctttaagatg tcgatgccgg gcagatgtcc gtcattttca
     2461 atcatttcaa gagaggcgcc accaccggtg gaaacatgcg agaaggcatc tgagaagccg
     2521 aactctttaa cagctgcggc cgaatcgcca ccaccgcaga cggtgaaagc gctcttcaat
     2581 ttagcgatag cctggcagac ggcaatcgtg ccgccggcaa aatctttttg ttcaaagaca
     2641 cccatcggac cattccagaa aaccatcttg gctttgctga tttcctgttg gaacaaagca
     2701 gcggtcttgg gaccgatgtc catgccttcc atctcgccgg gaatattggc gtcgtcggta
     2761 acgatttttt gcgtccagcc ttcaaaggcg tcagtggcga ttgaatcaag gggcaaaaga
     2821 atgcggccgt ttgcttcttc aaagcacttt ttggcgtatt ctagctgatc atcttcgcac
     2881 ggcgacttgc cgatggtgcg acctaaggct ttcgcgaacg tataagccat cgctccgccg
     2941 atcagaatct tgtcgcattt cttaagaagt gagtcgataa ctttgatttt atcggaaact
     3001 ttaaaaccgc cgaggatagc aatgtaaggg cggtcgccgt ctttaacttc gacacagcgg
     3061 gtcagattgc ggacttcttt ttcgacgaga tagccgatgg cgaccggctt gccctgagct
     3121 ttgagaattt ccgggacccc gtatgtagaa gcgtgggcgc ggtgggcgct accaaaggca
     3181 tccatgacga aaatatcgat gttttcagcc catttcttgg cgagttcagg atcatttttt
     3241 tcctcgcctt tttcgtagcg agtattttcc attaaaacga cttcaccggg ttcgacaaca
     3301 cccataaaaa acttggcttt ttcgccggtt gtatccgggc agaaagcgac gggtacgcga
     3361 aggatttcac ctaagcgggc tgccaccggg cgcaagctgt ttttcttctt ctgcgcttcg
     3421 acgacttccg gcgcttcctt gtgtttgatt ttgccgaggt gcgaaagcag gatgactctc
     3481 gcgccatgcg agatgagcat cttgattgtc ggggcggctt ggacgatgcg gttatcgtcg
     3541 cggatttcgc cgtccttaag aggtacgtta aaatcgacgc ggactaaaac gcgcttacct
     3601 ttgagatcgc ttaattgatc aatcgtgagc ataattatgc ctccttataa acaaaaaggt
     3661 gcctttgcta aagcaccctt tgtcagcaac ttattttaaa cctaaaacct tggccatttc
     3721 cttggcaatg cgcaagtatt ggcaggtata gctgtactcg ttgtcgtacc aggcgacgac
     3781 tttgacgaac tgctggccat caggattggt gaagaccgcg gtttgcgtgg cgtcgaagat
     3841 tgagccggct gtggcgccga tgatgtcgct cgagacgatt tcgtcctcgg tataagcaag
     3901 gacgtctttg agataagtgt cggccgcttc tttcatcgcc gcgttgattt cttcgacggt
     3961 gacatcttta ttaaggagca gcgagagatc gactaaggaa ccatcgatga ccggaacgcg
     4021 gagggcaccg ccttgaaggc gacccttgag ttcgggaatg acgaggttga tggctttggc
     4081 ggcgccggtc gtcgtcggaa cgatgttggc ggcggcagcg cggccgcggc ggctgttgcc
     4141 ttttttaact aagtcgagag ttgtctggtc attggtatag gcgtggacgg ttgtcatgta
     4201 gccgccttta ataccgaatt tgtcgacgag aactttggca accggagcta agcagttggt
     4261 cgtgcaggaa gcgccggaga tgacggtcat gtcctttgtt agtttgcctt cgttaacgcc
     4321 gtagacaaag gtcggagtct ctttatcggc gggagcggag atgacgacac ctttcgcacc
     4381 accttcggta atatgaacgg cggcatcggc tttacctttg aaacggccgg tgcactccaa
     4441 gacgagctcg gcgccgtaat cagaccattt gatcagcttg ggatcaggct ggccgataac
     4501 agggatagct ttgcccttgt aaacaattgc gtttttttcg acatcgacgg tgatgtcatc
     4561 gacaccgaag cgcttatgcg cggtatcgta cttgagaaga taggctagtg aagccgcatc
     4621 cgccaagtca ttgatggcga cgacttcgaa ttcgccggtc tcgtatgccc ttctgaaggc
     4681 gagacggccg attcttccga atccatttat agcgactttg attgacatat atatgtcctc
     4741 cttttttcta ccgcgtttat tatataagaa ttaggtcaat tatttaagta aaatcacttt
     4801 gtaagcgttt acgatttttt tatctgagac tcagcaaagg cataaaacaa gagttttata
     4861 cataaggaaa tgaggttatt ccgcgaaaga gggtctcgag attaatttcg aaggcaaagg
     4921 cgattttttc caaaaccttc aaagaaggat tgcggcgccc attttctaaa tcggagagat
     4981 aattgcggtt taagctggcg cgaaaggcca tctcctcctg attccagccg cgagcccgac
     5041 gcaaagcggc aattttttgt ccgagctgca gattagtttt catcatcatc ccctcgatag
     5101 cgcttagcga gaacgcggag agcgcggaag atatgattga catttgattt tgtcacgact
     5161 ttatccatct tctcgctgag catggcagcc aactcttcca tcgtcgcgtc ttcattttgc
     5221 aagcgaattt cagccagaag gcgctgcttc tcattagcga gatttttcac gccgagaatt
     5281 ttgtcaatcg tccgcaggtc atcgagctga cgggccgctg aagtcagcgt tttctcgtaa
     5341 ttggcggtgt cgagattttg caggcgattg ccaatgttgg caaattcgcg gtcaatacga
     5401 atattttcga attcgagact tgaagccgtg gcacccatca aaatgagaaa atcagcgatc
     5461 tggtcggact ttttaagata gatgacaatt tgctcgcgcc gcttgatggt cttgggacta
     5521 aagcttccgc ccttgtattt ggcgattaac tttaaaaacc atttcgcgta gttttcgtca
     5581 ttgagagcga tctcaaagtg gtagttggaa ctgcgcgggc tgttgaccga accggaggcg
     5641 agaaaagcgc cggctaagta accgccgatc atatcatcat taaagacgat attcttggca
     5701 attttgcctt ccaaaaagga aatttccaaa tcgcccataa tatattcggc ttcctgatcg
     5761 atgatgatgt tgtaagtaat acggcgttta aacttcattg ttttctcata agagaaacgg
     5821 atattgatgc catatatttg ctgtatgatt tgatagatga atttcgctat tttggcgttt
     5881 tcggtttgta aaagaatcgc aacgcagcga ttcttgattt ccatgcgacc gttgaccttg
     5941 atgaacgccg agactaaggc gcggagacgc tcttcggtaa aaggtttggt gacaatctct
     6001 tctttaactt tgcgggtaaa cgaaagcgtc ggtctcactt tttaaatttt ccgatatcgc
     6061 ggtgtaagca aagggttcgg taattggcgg cgtatttact ggccagatat tgcgcgatga
     6121 caaccgagcg gtgctgaccc ccgctgcagc cgatgccgac gacaaagaaa ttacggtctt
     6181 cggcgcggac cttggcgaga taataatcaa ggtatttctc aatattttta atcagttctt
     6241 tggcttcttt gtcttcgagg acgaacttct tgacgggctc atcgagaccg gtcatgtctt
     6301 ttaattcgct aatccaataa ggattaggaa cgatgcgggt atcaaaaatc atctcgacat
     6361 cattgggcac gccgtgcttg aagccaaagg agacgaaact aatcgtcagg ttgccgcttt
     6421 ccatgccctg tatgttggta aagatgaatt tgcggagttc agtcgccgtc ttgccggtcg
     6481 tatcgacata catatcgaca tcctttttca agacatccat gattttgcgg tcgcgttcga
     6541 gggaatcggc gagcgataat ccgtgagctt gcgccggatg gatgtggcgc gtcaaacgaa
     6601 agcgcgtcaa taattcggct tcagaagcat caagagctaa gaaagtgacg cggaaattgg
     6661 tatttttacg ggcggcctga gcggctttta aagcctcatc aatagccacg acgacgacgg
     6721 ttcgccgata gcggaccgga tcatcttcaa atgctttata aagcttagga ataacggaaa
     6781 aagggacatt ttcgacgatg taataatcca tttcttcgaa tgtccttacg gcagttgatt
     6841 ttccggctcc cgagacgccg gtcaagataa ttaagtcaat cataaaa
//
LOCUS       DAOSSL010000039         6614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_10709_length_6614_cov_22.984601, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000039 DAOSSL010000000
VERSION     DAOSSL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10709_length_6614_cov_22.984601"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..2250
                     /locus_tag="PLH17_04135"
     CDS             226..2250
                     /locus_tag="PLH17_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOR20991.1"
                     /translation="MTKELRKMKIRALFIVALELISAAALAVFYFYDFFNFRDIYIIE
                     YLFATLAGFVVINVLFIWVMLVRLSKIRKKSDLKAAELIGNDVQEAYKFGMIGLVVVD
                     ENDIVLWTNDLFQERQIDLLDINILEWQPNLRELHDASPDVVVKIEVNSRNYDVKYLS
                     DAGLYIFKDMTEYESIFEYSREQAPVLGIIMLDNYSDVAGNLDDANDVISKVKNLIFD
                     YAKEYGVLLRRYRNDAYFALCNYSSLNRMKKDRFSLLEKVRELGAKEETPPTLSIGLA
                     HDFPDVVKLNEMAGNAIDIAMSRGGDQVVVSKYGDELIFFGGKSEAQEKRNKVKVRVM
                     ADSVLSLIKNSSNVIIMGHTAMDMDALGACLGMRAICEYCNKSSHIVYDPKLLERKTK
                     FALTGAFSREELARITISPSDSVDKVRSNTLVIVCDVHRPSLTMAPKLLEKATKVMVI
                     DHHRRAEEFIESPVFSYVEPSASSTCELVTELIRYSSANPRIEISPTYATIMLSGIFL
                     DTTYFKSKNTGIRTFEASMVLKEYGADNSVADDYLKDEFEEYSLVTKIISTLKTPHYG
                     VVYCVAEESELIEISTLAKVANQCMQMKGVNAAFVIGNTDEKETRISARSDGTINVQM
                     LAEKMFGGGHFTMAGVSFKNGTIKKAEEKLLEVLNEYLNDARSQEQKARA"
     gene            2252..2701
                     /gene="rplI"
                     /locus_tag="PLH17_04140"
     CDS             2252..2701
                     /gene="rplI"
                     /locus_tag="PLH17_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOR20992.1"
                     /translation="MRVILLQDVKNVGKKGQSVEVNDGYASNFLIPRRLAVIETTRSV
                     EVLNKQIQDKKDAEAEAKAQAQEVAKKLEKITLEFAVQVGADQKMFGAISAKQIEAEL
                     KGRHNIVIDKRRFIDKVAINNLGYSRLRLDLFKGVEGIVNVHVVKKE"
     gene            2704..4197
                     /gene="dnaB"
                     /locus_tag="PLH17_04145"
     CDS             2704..4197
                     /gene="dnaB"
                     /locus_tag="PLH17_04145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOR20993.1"
                     /translation="MAKARNSLPNNNEAEKAVLGSMLLSPQAVVDGLGSLAFEEFFEG
                     NYANRTVFKAMRQLQDKHIPIDVQTVTEELINSKELDSIGGTDYLLELSQSAISLSNL
                     EFYIKIIKDQAVLRNLLLTLRKIDEEYLEKQIDDITDFVSDAEVRVRKVTEKRRVSNF
                     IGSGELAERVRESINLQTKKATEDGVTGMNTGYTKLNALTQGFQKDNLIIVAARPGMG
                     KTAFALNVAFNVSLKNNLPVAIFSLEMSSDSLAKRLVANRACVPLEDISRGYLPPKSR
                     VAIEEAINVISRSTIFIDDTPGIKLNDIIAKSRKLHSTHGELGLIIVDYIGLITSGAK
                     KFENRQQEVSEISRSLKDLARELKVPVIAVSQLSREVEKRDDKRPQLSDLRESGSIEQ
                     DADLVLLLFRRDYYESMGVKLGARRRRFGGDGQPEEKTKEDKIRELDAKLPGDASLVE
                     ISIAKNRNGKVGVVPLHFFKSYGRFDNPTREYEEDLARIEAEDGGSA"
     gene            4197..4952
                     /locus_tag="PLH17_04150"
     CDS             4197..4952
                     /locus_tag="PLH17_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOR20994.1"
                     /translation="MRFYIIASGSKGNATLLEHDGRVLLIDMGITLSRLKSELEVIGY
                     NIKDIEAVLFTHEHSDHASGARFFDEDVIYATEGTLGGSKHHILKPYEKINILGLNVT
                     PLATSHDAFAPIGFLFENSIEKLVYMTDTGFISEKNLEVMRNADYYIIESNHNIKMLL
                     QTNRPAELKQRILSDFGHLSNEDSALYMSELIGDKTKEIVLAHISEEANDNETALGTY
                     LRILKKRFVDIDKIRIYCAEQWSMIQGGQAREN"
     gene            4942..5421
                     /gene="rlmH"
                     /locus_tag="PLH17_04155"
     CDS             4942..5421
                     /gene="rlmH"
                     /locus_tag="PLH17_04155"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015880244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOR20995.1"
                     /translation="MKIKIIAVGSIKEDYWRVALEEYTNRLSLYAKVEICEVKDEPAP
                     PHMSKVLQEKVKLKEGTKILNKIKSKDLVVAIDLNGPQRDSETFAKYLDSLFVRGNSE
                     IVFVIGGSLGLSPEVQSRADEVVTLSKMTFTHQMTRVILLEQIYRAFKINRNETYHK"
     gene            complement(5431..5952)
                     /locus_tag="PLH17_04160"
                     /pseudo
     CDS             complement(5431..5952)
                     /locus_tag="PLH17_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT         1872 a         1626 c         1501 g         1615 t
ORIGIN      
        1 gggagaggtt tacagcaggc gtcccttttt ggaaaaacgg acttgtccca tcacaagaga
       61 gctcctaatc ggggctcttt ttttatgccc tcttgcacgc aaataagccg atttcgcata
      121 aatgcgacac ggtattatgg cgaatcaagt caagatttct ttattaatac tttatacttt
      181 tttagtattc gttatttgtt ataattagag acagccgagg gtaatatgac caaagaactg
      241 agaaaaatga aaatccgcgc tttgtttatc gtggcgctgg aattaataag cgctgccgct
      301 ttagcggtct tttactttta cgatttcttt aatttccgcg acatctacat catcgaatat
      361 ctttttgcca ctctcgccgg ctttgtcgtc atcaatgtct tgttcatctg ggtgatgctc
      421 gtccgcctgt ccaagattcg caaaaagagc gacttgaaag ctgccgaatt aatcggcaac
      481 gacgttcaag aagcctataa attcggtatg atcggtctcg tcgtcgtcga cgaaaacgac
      541 attgtcttat ggaccaacga tcttttccag gaacggcaaa tcgacttgct tgatatcaac
      601 attctcgagt ggcagcccaa ccttcgcgaa ctgcatgacg ccagtccgga tgtggtggtc
      661 aaaatcgaag tcaactcgcg taactatgac gttaaatatt taagcgacgc cggcctttat
      721 atatttaaag atatgaccga atacgaatcg atttttgaat actcccgcga acaggctccc
      781 gtcctcggca tcatcatgct cgataattac tccgatgtcg ccggaaatct cgatgatgcg
      841 aatgatgtca tttctaaagt caaaaaccta atctttgatt acgccaaaga atacggcgtc
      901 ttgttaaggc gttatcgcaa tgacgcttat tttgctctat gcaattacag ctcattgaat
      961 cgcatgaaaa aggaccgctt ttcgctcctt gaaaaagttc gcgaattagg cgccaaggaa
     1021 gaaactccgc cgacgctttc aatcggcctc gcccacgact ttcccgacgt cgtcaaactt
     1081 aacgagatgg ccggtaacgc catcgacatc gccatgtcgc gcggcggcga tcaggtcgtc
     1141 gtctctaaat acggcgatga actgattttc ttcggcggca aaagcgaagc ccaggaaaag
     1201 cgcaataaag ttaaagtccg cgtcatggcc gactccgttt tatctttgat aaagaactca
     1261 agcaatgtca tcatcatggg ccacaccgcg atggatatgg atgccctcgg cgcctgcctt
     1321 ggcatgcggg caatctgcga atactgcaac aagtcatcgc acattgttta cgacccgaaa
     1381 ctcttggagc ggaagacgaa attcgccttg acgggagcct tctcgcgcga ggaactcgcc
     1441 cgcattacca tctcgccgag cgacagcgtt gataaagttc gctccaacac cttggtcatc
     1501 gtttgcgatg ttcaccgccc gagcctcacg atggcgccga aattactgga aaaagccacc
     1561 aaggttatgg tcattgacca tcaccgccgg gccgaagagt ttattgaatc gcccgtcttc
     1621 agctatgttg agccaagcgc ctcttcaaca tgcgaattag tcactgaatt aatacgttat
     1681 tcttcggcta atccccgcat tgaaatatca ccgacatatg cgacgattat gctttccggc
     1741 atcttcctcg acaccactta ttttaaatcg aaaaacaccg gcattcggac tttcgaagct
     1801 tcgatggttc ttaaagaata cggcgcggat aactctgtcg ccgacgatta tttaaaagac
     1861 gaattcgaag aatattcgct ggtcaccaag attatctcca ccctcaagac tcctcattac
     1921 ggcgtcgttt attgcgttgc tgaggaaagt gaattaattg agatatcaac cttagcaaaa
     1981 gtcgccaatc aatgcatgca gatgaagggc gtcaacgccg cctttgtcat cggaaacacc
     2041 gacgaaaaag aaacgagaat ttcggcgcga agcgatggca cgatcaacgt tcagatgctg
     2101 gccgaaaaga tgtttggcgg cggccatttt acaatggcgg gtgtatcatt taaaaacggg
     2161 acgatcaaga aagccgaaga gaaacttctc gaggtattaa acgaatattt aaacgatgct
     2221 cgttcgcaag agcagaaagc gagggcataa catgcgtgtt attttgttgc aggatgtaaa
     2281 aaatgtcggt aaaaagggtc agtccgtcga agtcaacgac ggctacgcta gcaatttttt
     2341 gattcccagg cgcctcgccg tcatcgaaac gacgagatcc gtcgaagttt taaacaagca
     2401 gattcaagac aagaaagacg ccgaagcgga agcgaaagcc caggctcagg aagtcgccaa
     2461 gaaacttgaa aagataacgc ttgaattcgc cgtccaagtc ggcgccgatc agaagatgtt
     2521 cggggcgatt tccgccaagc agatcgaagc cgaactaaag ggcagacaca atatcgtgat
     2581 cgacaagcgc cgctttatcg acaaagtcgc gatcaataac ttaggttata gccgccttcg
     2641 cctcgacctt tttaaaggtg ttgaaggcat cgtcaatgtc cacgtcgtca aaaaggagta
     2701 agtatggcta aagcacggaa ttcactacct aataacaacg aagccgaaaa ggccgtctta
     2761 gggtcgatgc tcctctcccc gcaagcggtg gtcgatggtc ttggctcgct tgcctttgaa
     2821 gaattctttg agggcaacta cgccaaccgc accgtcttta aggccatgcg ccaactgcag
     2881 gataagcaca ttcccatcga tgtgcaaacc gtcaccgagg aattgattaa ttctaaagaa
     2941 ttagactcaa tcggaggcac cgattacctc cttgaattat cgcaatcagc catctcctta
     3001 tctaatcttg agttctatat caaaatcatt aaggatcaag ctgttcttcg caatctttta
     3061 ttaacccttc gcaagattga tgaagaatat cttgaaaagc aaatcgacga tatcaccgac
     3121 tttgtctccg atgccgaagt ccgcgtccgc aaggttaccg aaaagcgccg cgtctcgaat
     3181 tttatcggca gcggtgaact cgccgaacgc gttcgcgaaa gcatcaacct ccaaacgaaa
     3241 aaagccaccg aagatggcgt gaccggaatg aacaccggct acaccaagct aaatgctctt
     3301 acgcaaggtt ttcaaaagga taatttaatc attgtcgccg cccgccccgg catgggcaaa
     3361 acagcctttg ctttaaatgt cgcctttaac gtctcgctta aaaacaatct tccagtcgcc
     3421 atcttctctc ttgagatgtc ctcggactca ctggccaaga gattggttgc taatcgcgcc
     3481 tgcgtcccgc ttgaggatat ctcgcgcggg tatttgccgc ccaagagccg cgtcgccatc
     3541 gaagaggcca ttaatgtgat ttcgcgctcg acgattttta tcgacgacac gcccggcatc
     3601 aaactaaatg acatcatcgc caaatcgcgc aaattacata gcacgcacgg tgagctggga
     3661 ttaattatcg tcgactatat cggcctcatt accagcggag cgaagaagtt cgaaaaccgc
     3721 cagcaggaag tcagtgaaat atcccggtcc ttaaaggatt tagcccgcga actgaaagtt
     3781 ccggtcatcg ccgtttccca gctctcccgc gaagttgaaa agcgcgacga taagcgcccg
     3841 cagctctccg acctgcgcga atccggatca attgagcagg atgccgactt agtcttgctt
     3901 ttattccgtc gcgactacta tgagagcatg ggtgttaaac tcggggcccg ccgccgcaga
     3961 ttcgggggag acggacagcc cgaagaaaag accaaggaag ataaaattcg cgaacttgac
     4021 gccaagcttc cgggcgatgc ctcgctcgta gaaatttcga ttgccaagaa ccgcaacggc
     4081 aaagtcggcg tggttcccct ccacttcttt aaaagttacg ggcgctttga caatccgacc
     4141 cgcgaatacg aagaagattt agccagaatt gaagccgagg acggcggatc agcctgatgc
     4201 gcttttatat tatcgccagc ggctccaaag gcaacgccac cttattagaa cacgatggtc
     4261 gtgttctttt aatcgatatg ggcattactt tatcgcgcct taaaagcgag ctggaggtca
     4321 tcggttacaa tatcaaagat attgaagccg tactttttac tcacgaacac agcgaccacg
     4381 ccagcggagc gcgcttcttt gatgaagacg ttatctacgc caccgaagga acgctcggag
     4441 gtagtaagca tcacattctg aagccttatg aaaagataaa tattctcggt ttgaatgtca
     4501 cccctctggc gacttcgcat gatgcctttg ccccgattgg ttttctcttt gaaaacagca
     4561 tcgaaaagct cgtctatatg accgacaccg gctttatcag cgaaaaaaac ctcgaagtca
     4621 tgcgcaatgc cgactactat ataattgaat caaatcacaa tatcaaaatg ttgctgcaga
     4681 cgaatcgccc cgccgagtta aagcagagaa tcctcagcga tttcggccat ctgtccaacg
     4741 aagactccgc tctttatatg agcgaattaa tcggcgacaa aaccaaagaa atcgtgctcg
     4801 ctcatatatc cgaggaagcc aacgataacg agacggctct tggcacttat ctacgaatct
     4861 taaaaaagcg tttcgtcgac atcgacaaaa tccgcatcta ctgtgcagag cagtggtcga
     4921 tgattcaagg aggacaagcc cgtgaaaatt aaaattatcg ccgtcggaag catcaaagaa
     4981 gattattggc gcgtcgccct ggaagaatac acaaaccgcc tttctttgta cgccaaggtc
     5041 gagatttgcg aagttaaaga tgaacccgcg cctccgcaca tgtcaaaagt ccttcaggaa
     5101 aaagtaaaac tcaaagaagg aacaaaaata ttaaataaaa ttaagtcaaa agacttggtt
     5161 gtcgccatcg atttgaacgg cccgcaaaga gatagcgaga cgtttgcgaa atatctcgac
     5221 agcctctttg ttcgcggcaa ctccgaaatc gtctttgtca tcggcggctc cctcggctta
     5281 agccccgagg ttcaaagccg ggccgatgaa gtcgtcacct tgagcaaaat gacctttacg
     5341 catcagatga cccgcgttat tttgctcgag caaatttatc gcgcgtttaa aattaatcgc
     5401 aacgaaacat atcacaagta aattggtgat ttaagttata tcttttgcgt cgatgtagca
     5461 gacgcggttt ttgccttggg acttagcttt atataaactc ttatcggcgc gtttaatgag
     5521 ggtgacgctc gtgtccccga gctcataaat ggcgacgccg atgctgatcg aaattttccg
     5581 cggctgaaac aggtcatttt cttcaaccgt ccttcgcagc ccatccgcgc atttaagggc
     5641 ggcctcggga gtggaagact cgcagaagac aataaactcc tcgcctcccc agcgaccgat
     5701 attgacgcgg tcgcgacccc tcataagatt tgagaaggcc ttcaaagcct agtctccggc
     5761 gacatgtccg aattcgtcgt tgatttgttt aaaatcgtcg aaatcaacga cgaaaagcgc
     5821 aaatccagca ccttttttgc ctttttccaa acgcttttcc agctcctcgt caatccattt
     5881 ccgattgtat aaacctgtca gggcatcggt gatggcgact tcttcaaggg cccgatttgt
     5941 ctgtatcagc atgccatggg cttccactag agatgtctcg taaacaagga ttaaggccat
     6001 tgatgaaagc gatcctaaaa tgaaattagt cagggcgccg agatcgaatg ttatttcgcc
     6061 cacgatatca ggctgtaaaa tcagatagac aattgtgatg gcgaaggcga tggcagagac
     6121 aattgcgcct ttgacacgcc caatcagata gaaggcgatg attgaaacga cggggaataa
     6181 gtaaagaaca taagcgacgt tgattgatag ttcaaggtag acaagataag tgatgagaac
     6241 aatgccgacg agggcataac aggtgatgct cgcctttttc caaatcagat agaagataaa
     6301 ggttaaaacg gtggcgagaa gcgaaaaaga aaaccacagc gttccgccga gcgaccagaa
     6361 tattctggta ttgatgatga taccgagcat aaagccgacg cttaaaaacg aaaaagtgac
     6421 gaggagcaag cgcttcttgt taattttggc gtattcactg aaaccgatat ctttgtctag
     6481 ttttggtttt tcttgcaatt caattagtcc tcgtgaaaaa aattatcaat ttttaatatt
     6541 ttaccacttt taacacatgt aaagcgataa attgccatct ccggcctctc gtgataatag
     6601 caacgtaaaa atct
//
LOCUS       DAOSSL010000040         6463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_11031_length_6463_cov_15.100343, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000040 DAOSSL010000000
VERSION     DAOSSL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11031_length_6463_cov_15.100343"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..763)
                     /gene="nadE"
                     /locus_tag="PLH17_04165"
     CDS             complement(20..763)
                     /gene="nadE"
                     /locus_tag="PLH17_04165"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOR20996.1"
                     /translation="MEFRQYLGEIEKFLQQYLINARAKGYVLGVSGGIDSALVAALTT
                     KAVGRDKLFALLMPIDSHPSDLQDGLELCQKFDIPHRVIDLSEPYHTLIKNFAFADDE
                     AAKLALHNTKVRLRMVTLYAYAQSRGALVLGTDNADEIHVGYFTKYGDGAADLAPIAE
                     LTKGEVYLASKILGVPESILKRTPTAGLFSGQTDEGELGVTYAELDDYLLGRKVDQKV
                     AERIQYLYRISEHKRQPIPRPIKYARNND"
     gene            complement(776..2053)
                     /gene="obgE"
                     /locus_tag="PLH17_04170"
     CDS             complement(776..2053)
                     /gene="obgE"
                     /locus_tag="PLH17_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010688832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOR20997.1"
                     /translation="MFIDRVKLTVKSGKGGDGLIAFLHEKYMPKGGPSGGNGGRGASI
                     ILKASSSVNTLFGLYRKKIIRGEDGAKGGIKNQYGRGAKDVYVEVPIGTIVYEADGHN
                     FLYDLNEEGAEFVVAKGGRGGRGNAAFKSSRNRVPRIAENGVPGEEKDIILELKLLAD
                     VGLVGFPNVGKSTLLSVVTNANPEIADYPFTTITPNLGVVSVDSSTSFVMADLPGLIE
                     GAHQGRGLGLTFLRHIERCRVIIHMIEMSGIRDPYDDYQAINVELEKYGYNLTKRPTI
                     ICGTKMDEDGAEERLLALEKKLKTKIYGISALTHEGLQELMYAASELVKQTPLFPIVS
                     QEGAIETIRVYDAYKDGAGDFEIVLEKEGVYRIVGEKVERTYHLINLTTDEGLLKLMT
                     YLRKIGVEERLQKVGARDGDTVVLCDFEFDYIE"
     gene            complement(2057..2785)
                     /locus_tag="PLH17_04175"
     CDS             complement(2057..2785)
                     /locus_tag="PLH17_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOR20998.1"
                     /translation="MEIKKLSIIVPCYNEGKKIRRNLEDKVLPFLRERRINFEIILVN
                     DGSKDNTKELFNVLVLENVRPLGYDQNRGKGGAVKYGIEQATGDYILFMDADLSTDLK
                     ALDLIYADQDNADMYIGSRHLKESVLAKKQGLMRRFVGRSCRLIVNTAFKFKFKDTQC
                     GFKLIRADYAKEMADRQIINGFAFDVEYLYMARLNNLKIKEIPVIWENDEDSKVSTFK
                     SSIRFFKDVRIIKKNKSNYLFASK"
     gene            complement(2842..3126)
                     /gene="rpmA"
                     /locus_tag="PLH17_04180"
     CDS             complement(2842..3126)
                     /gene="rpmA"
                     /locus_tag="PLH17_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOR20999.1"
                     /translation="MIYTLNLQFFASKKGVGSTKNGRDSHSKRLGAKKSDGEFATAGS
                     ILYRQRGTKVYPGVNAMRGGDDTVFATVDGIVKFERVGKSRKRISVYEVK"
     gene            complement(3123..3446)
                     /locus_tag="PLH17_04185"
     CDS             complement(3123..3446)
                     /locus_tag="PLH17_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOR21000.1"
                     /translation="MIKVTCEVKNSQIASLEIKGHAQSAEPGQDLVCSAVSAVTFGGL
                     NALRNTQQNLNIEVQEGYVLITRSGEVESADEVVLTTILTQLETIARDYPKYVKILQK
                     GKLKQ"
     gene            complement(3449..3757)
                     /gene="rplU"
                     /locus_tag="PLH17_04190"
     CDS             complement(3449..3757)
                     /gene="rplU"
                     /locus_tag="PLH17_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002853550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOR21001.1"
                     /translation="MYAIIETGGKQVKVEVGQKIYVEKLDVAVDVNYTFDKVLLIGDK
                     QAKVGSPYVKDASVVAKVVKQGLSKKLRVFKYRNKHNERKTIGHRQPYTCLVIEAING
                     "
     misc_feature    complement(3776..3853)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(3951..4628)
                     /gene="radC"
                     /locus_tag="PLH17_04195"
     CDS             complement(3951..4628)
                     /gene="radC"
                     /locus_tag="PLH17_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOR21002.1"
                     /translation="MRIKDLPEAERPREKALRYGIASLSNIEILALVIGSGVRDQSAL
                     DIAAHLIAEAKGIHHIENCSLERIEKIRGIKKISALRLAAVFELLKRVEKTRLECVSE
                     PVHPKAIFEKYKLEFARETQEQFALLFLSRRRMITGEKIMYKGTAEYFPIAIGEILSE
                     LLANKCFAFIVVHNHPSGETQPSDDDLISTKMLASEAARLKIKLVDHIIISDRSYFSF
                     AEHNLIK"
     gene            complement(4635..4817)
                     /locus_tag="PLH17_04200"
     CDS             complement(4635..4817)
                     /locus_tag="PLH17_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21003.1"
                     /translation="MNLQIMSLEEMEQHVTGEAITLSAVMAIAAIAIVAVVVYRLFMS
                     DKGSTTLPGGFKFEWK"
     gene            complement(4877..>6463)
                     /locus_tag="PLH17_04205"
     CDS             complement(4877..>6463)
                     /locus_tag="PLH17_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOR21004.1"
                     /translation="KITHSVGHSERTDSVVEPYLSKQWFIKMRPLADRALQDSEVDFI
                     PKRFQKIFRNWMENAEDWCISRQLWWGHRIPVYYHKVTGAEVCSVDTPPDVENYRQDE
                     DVLDTWFSSALWPFATLGWPNLTDDYKRYYPNSVMVTAYDIIFFWVSRMIFQGLEFTG
                     KSPFEKCVIHGLIRDAKGRKMSKSLGNGIDPLEVIDQHGVDALRYFLMSSTAPGQDTR
                     YIEEKVVAGSNFLNKIWNSARYVLALLGDDFAPEKINFQDLAPLELHILQKHEKTIAA
                     VTRNMDKYQFSMALKHLYSYVYADFCSRYLEMTKVALGLNDQALSSSTKQVLYKVLKD
                     IVVMMAPYTPFVAEELYLALPAHRESVMLEPYPQMERIKGVRKHHEAVDDLYQSIQDI
                     RNFKVERNLPPNHPLSIKIRANKEPFANFSRYLARFAFAKQLVIEAEINALKGDVAFF
                     YSSFDLLIEEAVDKSELLAKLAKEEERLRFEISRSEAMLANERFLSKAPSDKIRLEKE
                     KYLKNKDLLGAVMKKIADLK"
BASE COUNT         1626 a         1502 c         1494 g         1841 t
ORIGIN      
        1 taaataaaaa gtaaacagtt taatcgttat tgcgggcgta tttaatcggg cgcggaatcg
       61 gctggcgttt gtgttcgctg atacgataga gatattgaat gcgctctgcg actttttgat
      121 cgacttttcg gcctaacaga taatcatcta attccgcata agtaacacct aattcacctt
      181 catcggtctg accgctgaat aaaccggcgg tcggcgtccg cttcagaatg ctttcgggca
      241 ctccgaggat tttcgaagct agataaactt cgcctttggt cagttcggca atcggcgcca
      301 aatcggcagc gccatctccg tatttcgtaa aatatccgac atgaatctcg tcggcgttat
      361 ccgttcctaa aacgagggcg ccacggcttt gcgcataggc ataaagcgtc accatcctca
      421 atcttacttt cgtattgtga agggcgagct ttgcagcttc gtcatcagcg aaagcaaaat
      481 tcttaatcaa agtatgatat ggttcactta aatcgataac gcggtgcgga atgtcaaact
      541 tttgacaaag ttcaaggccg tcttgtaagt ctgacggatg tgaatcaatc ggcatcagga
      601 gggcaaaaag tttatctctg cccaccgctt ttgtcgtcaa agcggcgacg agggctgagt
      661 caattccgcc cgagactccc aaaacatagc ccttcgcgcg ggcgttaata agatattgtt
      721 gaagaaactt ctcaatctcg ccaaggtatt gtctgaactc cataacgcac ctccgtcatt
      781 caatataatc gaattcaaag tcgcacaata cgaccgtatc accatctctg gcaccgactt
      841 tttgcaggcg ttcttcaact cctattttgc gcagatatgt cattaattta agaaggcctt
      901 cgtcagtcgt cagattaatt aaatgatatg tcctttcgac tttttcgcca acaattcggt
      961 agacaccctc tttttcgagg acgatttcga aatcgccggc accgtcttta taagcatcat
     1021 agacgcgaat tgtctcgata gctccttctt gagaaacgat tggaaagagc ggcgtctgct
     1081 taactagttc gctagcggca tacatcaatt cttgaagccc ttcatgggtc aaggcgctga
     1141 taccgtaaat tttggttttc agtttttttt caagagccag aaggcgctct tcggccccgt
     1201 cttcatccat ttttgtgccg cagataatcg tcggccgctt ggtgagatta tagccgtatt
     1261 tttccaattc gacattaatt gcttgataat catcataagg atcgcggatg cctgacatct
     1321 cgatcatgtg gatgatgacg cggcatctct cgatgtggcg aaggaaagtc aggcctaagc
     1381 cccgaccctg atgagcccct tcgatgagac ccggcagatc cgccatgaca aaagaagtgc
     1441 tgctatcgac gctgacaacg ccgagattcg gagtgatcgt cgtgaatgga taatcggcaa
     1501 tttcgggatt agcattggtc acaacactta aaagtgttga tttgccgaca ttcggaaagc
     1561 cgaccaagcc gacatcagct aaaagtttaa gttcgagaat tatgtctttt tcttcgccgg
     1621 gcacgccgtt ttccgcaatg cgcggcacgc ggttccgcga acttttgaag gcggcattgc
     1681 cgcgtccgcc gcggccgcct ttagccacga caaattcagc gccttcttca tttaagtcat
     1741 agaggaaatt atgaccgtcg gcttcgtaga caatcgttcc aatgggaact tcgacatata
     1801 cgtctttcgc accgcggcca tactgatttt tgatgccgcc cttggcgccg tcttcgccgc
     1861 gaataatctt tttccgatag aggccaaaaa gggtattgac cgaactcgat gcttttaaaa
     1921 tgattgaagc accgcgtccg ccattgccgc cgctcggccc gcctttcggc atgtattttt
     1981 catgcaaaaa cgcaatcagg ccatcgccgc ctttgccaga ttttacagtt agttttacgc
     2041 ggtcgataaa catatattat ttgctcgcaa agaggtagtt gctcttgttc ttcttgatga
     2101 ttcgcacatc tttgaaaaag cgaatactgc ttttaaaagt gctgacttta ctgtcctcat
     2161 cgttttccca gatgacggga atctctttta ttttgagatt attaaggcgc gccatataga
     2221 ggtattcaac atcaaaagca aaaccattga tgatttgcct gtcggccatc tctttagcgt
     2281 aatcggctct aattaattta aaaccgcatt gcgtatcttt aaatttaaat ttaaaagccg
     2341 tgttgacgat gaggcggcag ctcctgccga caaagcggcg cattaagcct tgcttcttgg
     2401 ccaaaacact ttctttaaga tggcgtgatc caatgtacat atcggcgtta tcttgatcgg
     2461 cataaattaa atcgagagct tttaagtcgg tcgataaatc ggcgtccata aaaagaatat
     2521 aatcaccggt ggcctgttcg ataccgtatt taaccgcacc gcccttgccg cggttttgat
     2581 catagccaag cgggcggaca ttttctaaaa ccaggacatt aaacaattct ttagtattat
     2641 ctttggatcc atcgttgacg aggatgattt caaaattgat gcgccgctca cgcaaaaaag
     2701 gtaatacttt atcctcgaga ttgcggcgta tttttttgcc ttcgttgtaa caaggcacga
     2761 taatcgaaag ctttttaatt tccatacata tattaaaaca caacgggatt aaaaaagacc
     2821 acatttttgt ggtcttatta gttatttgac ttcgtagacg ctgatgcgtt tgcgcgattt
     2881 gccgacgcgc tcgaatttga cgatgccgtc gaccgtggca aagacggtat catctccgcc
     2941 gcgcatggcg ttgacgccgg gatagacttt cgtgcctctt tgccgatata agatggaacc
     3001 ggctgtggca aattcgccat ctgatttttt agcgcctaaa cgcttggaat gagagtcgcg
     3061 gccgtttttg gttgaaccga cgcccttttt cgaagcaaag aactgcaggt ttaatgtata
     3121 aatcattgct ttaattttcc tttctgtaga attttgacat acttgggata gtcacgagcg
     3181 attgtttcaa gctgggtgag aatcgttgtt agaacaactt catctgcact ttcaacttca
     3241 ccgcttcgag taattaagac ataaccctct tgaacttcga tgttcagatt ttgctgggtg
     3301 ttgcgaaggg catttaggcc tccgaaggtc accgccgaaa cagcgctgca caccaagtcc
     3361 tggcccggtt ctgcgctttg cgcatgacct ttgatttcca agctggcaat ctggctattt
     3421 tttacttcgc aagtgacttt gatcataatt aaccgttgat ggcttcgatg actaaacaag
     3481 tgtagggctg acgatggccg attgtcttgc gctcgttgtg tttgttacgg tatttaaaca
     3541 ctctgagttt tttgctgaga ccttgtttga caactttggc gacaacgctg gcgtctttga
     3601 cgtaagggct gccgactttg gcttgcttat cgccgatgag caagacctta tcgaaggtgt
     3661 agtttacgtc gaccgcgacg tctagttttt cgacatagat tttctggcca acttcaacct
     3721 tgacttgctt accgccggtt tcaataattg cgtacattgg ttacctcctc ttttgatatt
     3781 cactaatgac tcgctatgga ggtcaaccat cgaagatttg ttaggaacct tttctaagcg
     3841 gttgtagctt gtgaggcgac aacgagaata ttataaacac attgcaaaag agagacaacc
     3901 ttttttaact aatgttaaga aaagttattt cgatgggggt tatcaacccc tcattttatg
     3961 agattgtgct cggcaaaaga aaaatatgaa cggtcgctga tgatgatgtg atcgacgagt
     4021 tttatcttta accgggccgc ctcgctcgcg agcatcttag tcgagattaa atcatcatcg
     4081 ctcggctgag tttcgccgct ggggtgattg tggacgacga taaaggcaaa gcatttattg
     4141 gcgaggagtt cagaaagaat ctcgcctatg gcgataggaa aatattcggc cgtcccttta
     4201 tacatgattt tttcgccggt tatcatcctt cttcgtgata gaaaaagaag ggcgaattgc
     4261 tcctgagttt cccgcgcaaa ttcaagtttg tatttttcaa atatcgcctt cggatgaact
     4321 ggttcgctga cgcactcaag ccgcgtcttt tcgacgcgct tcaagagttc aaagacagcc
     4381 gcgaggcgaa gggcggagat tttttttata ccgcgaattt tttcaattct ttccagtgag
     4441 caattttcga tatggtgaat gccctttgcc tcggcgatta aatgcgcggc gatatcaagg
     4501 gcgctttgat cgcgtactcc gctgccgatg acgagggcga gaatttcgat gtttgaaagg
     4561 cttgctattc cgtatcggag ggctttttcg cgcggtcgct ccgcctcggg caaatcctta
     4621 atgcgcacaa aaaactattt ccactcgaat ttaaaaccac cgggcaaagt cgtcgaacct
     4681 ttgtcggaca taaatagccg atagacgacg accgcgacga tggctatcgc agcgattgcc
     4741 atcaccgccg aaagcgtaat tgcctcgccg gtaacgtgct gttccatctc ttcaagactc
     4801 atgatttgca gattcataaa atgacctcct accgatatat aggaggccat tgagcaaaag
     4861 tgccgatttt aattaattat tttaagtcag caatcttttt catgacggca ccgagcaggt
     4921 ctttattctt caaatacttt tctttttcga gccggatttt atcagatggt gctttgctga
     4981 gaaagcgttc gttggcaagc attgcttccg aacggctgat ttcaaagcgc aagcgctctt
     5041 cttctttagc gagttttgct agcagttcag atttatcaac cgcctcttct atcagcaaat
     5101 caaaactcga gtagaagaat gcaacatcgc cttttaaggc attaatctcg gcctcgatga
     5161 caagttgttt ggcgaaggca aaacgcgcta aatatcgcga aaaattggca aatggctcct
     5221 tatttgcccg aatttttata ctcagtggat gattgggagg caagtttctt tcaactttaa
     5281 agttgcgaat atcctgaata gactgataca aatcgtcaac ggcttcgtga tgtttgcgca
     5341 cccccttaat tctctccatt tgcggatagg gctcgagcat caccgactcg cgatgggcgg
     5401 gtaaggccaa gtagagctcc tcggcgacaa acggcgtgta aggcgccatc atgacgacta
     5461 tatctttaag gaccttatag aggacttgct tcgtcgatga actcagcgcc tgatcgttta
     5521 atccgagcgc gactttggtc atttccaaat accgcgagca aaaatcagca tagacatagc
     5581 tatagagatg tttaagagcc atcgaaaact ggtatttatc catatttcgc gtaaccgcgg
     5641 caatcgtttt ttcgtgtttt tgaagaatgt ggagttcaag aggggcgaga tcttgaaagt
     5701 taattttttc cggcgcgaaa tcatctccga gcaaagccaa aacatagcgg gccgaattcc
     5761 agattttgtt taagaaattg ctgccggcaa caactttttc ttcgatgtag cgggtatctt
     5821 gaccgggagc tgtgctgctc atcaggaaat agcgtaaggc gtcgacgccg tgctgatcga
     5881 tgacctcaag cggatcgatg ccgttgccga gcgatttgga catcttgcga cctttggcat
     5941 cgcgaatcaa gccgtggatg acgcattttt caaacggcga tttgccggta aattcgagac
     6001 cctggaaaat catgcggctg acccagaaga agatgatgtc ataagccgtg accatgaccg
     6061 agttgggata gtagcgttta taatcatcgg tgagatttgg ccagccgagc gtggcaaacg
     6121 gccagagggc tgacgaaaac catgtatcga gaacgtcttc atcctgacga tagttttcaa
     6181 catctggcgg cgtatcaacc gaacatactt ccgcgcccgt tactttgtga taatagacgg
     6241 gaatccggtg accccaccac aactgccggg agatgcacca gtcttcggca ttttccatcc
     6301 agttgcggaa aatcttctga aatctcttcg ggatgaaatc gacttcgcta tcttgaaggg
     6361 cgcgatcggc aagcggtcgc attttgatga accactgctt gctaagatat ggttcgacga
     6421 cgctatcggt gcgctcgctg tgaccgacgg aatgagtgat ttt
//
LOCUS       DAOSSL010000041         6459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_11043_length_6459_cov_15.147720, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000041 DAOSSL010000000
VERSION     DAOSSL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11043_length_6459_cov_15.147720"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLH17_04210"
     CDS             <1..402
                     /locus_tag="PLH17_04210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HOR21005.1"
                     /translation="EDEQYLVGFKTEYEKEAFLSLISVKGIGPRTALGAMSATSAEEL
                     FNAIAANNITFLKKLPGIGAKAAAQIILDLKGQLTGSPSGNPNQYDEVRDALKGLGYK
                     IKEIENVLASINEKDATNEDILRLALKKLKK"
     gene            402..1409
                     /gene="ruvB"
                     /locus_tag="PLH17_04215"
     CDS             402..1409
                     /gene="ruvB"
                     /locus_tag="PLH17_04215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOR21006.1"
                     /translation="MSDRILDAKKNNKEEEVEISLRPKVLKEYVGQEELKRNLRVFMG
                     AAMHRDETLDHVLLYGPPGLGKTTLAYVIANEMKGNIRVINGPSIERAGDLASILADL
                     KAGDILFIDEIHRLPRIVEEVLYGAMEDFKLAILIPREQGSNTLNLTLEPFTLIGATT
                     RAGDLSSPLRSRFGIVEKINFYSEKEIEGIVRRTSKVLKTEIDEKAVSLIAKRSRGTP
                     RIANRLFRRVRDFANFEGKDTIAEIDAKKALDALKVDNLGLDDVDVRYLSAIIERFHG
                     GPVGLEAVASAIGEEIMNLEDVYEPYLLQIGMINRTPRGRVVTEKAYAHLKKNHQKSL
                     F"
     gene            1605..4223
                     /locus_tag="PLH17_04220"
     CDS             1605..4223
                     /locus_tag="PLH17_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21007.1"
                     /translation="MRRLIAYITLAVSSIIAIGVGINPIMSKGINNLDYRDGRQFVYQ
                     LFEKDDETIDVDAEDAAREVAKDMDERLKTWGVSNYKVEIEGNDIVRVSLTAKNDTEY
                     DYLERYLGFSGQDFSIATADESVRLTHDEVFKDSEAYIVYEGYAPYVIIPLSNPSEFK
                     TLVDAANQAMNDDIDTVDDDEPQYRKSKAEGDEEQQSEPNIYLWANWAEGEDYAKSQE
                     DQKVADKIIAQFLSTNIYYENSDVKETEIKYVSGFADEEGNYDTSKIRQANTLALFVK
                     NLFNANSYDYKVVNIFSTPIVAEVESILTYGMSVSLSISKTLIATLVVAAVVALVLAF
                     FFRIGAVSVMTSTAVSFFATLAIFVAFRAEFNIAGLLALLLVAAASLVAGILHLTKFK
                     EEVYRGRNFKKANTEAAKAMTVPAIDIAVIMVAVGVLSYVIGGALVASFGTMLVIGGV
                     VSLAVNLIILRVMTWLVTNTTALQGNNKVFNIEDKNVPNLLKEEKQTYFGPYQDRDFT
                     KRAKPVAIGVGAFSLIAIITMSIFGAINGSIYNANPVYEETSRAYITVYSDSPVVDSV
                     TYIEDNILKFIYVDGELVSYSSIDHETREDYDAETTITTTYNYYIVNFDKAYSGEEDA
                     YFEGDPLSEGMSLEGVLDAYIDSVEGGEGLVRIKVSENLVMQPSTLHIALGALAGVAL
                     AAFYLAFRYRISRALAAFSVSAIASFATLGFFVVTRIATTPIVALVVPVVALFSLFAS
                     LYFLIRERDLLKEDRSKEKTAQVRKEIMIKATALGAGPLFVFGIIASYFAINYFGFGP
                     ANFAVLFGGMLLGVALSVLLIVTLLGPSALFFEELIGKIVARIRVPRLRKSKKRVAPK
                     STSSEPEETIFIGIND"
     gene            4262..5827
                     /locus_tag="PLH17_04225"
     CDS             4262..5827
                     /locus_tag="PLH17_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOR21008.1"
                     /translation="MDFLNDLLNYYQLSKEQYEIMTRPLSYGDLRFDYRVKGLEPLLV
                     RIKKAIASKEKIIVYGDYDCDGIMSTSIIVKVFELLGHPIGYYVPSRYIDGYGLNVKR
                     VEEIAKKGYSLIITVDNGISAFEAISLAKEKGIDTLVVDHHEVQDDKLPDAVGILHPF
                     VSELGEYAASGGFMAFVLATALLDKVEPYLLALAGISTISDMMPLLGYNRDLVRLAIE
                     TMNRNHFPSIARLTGDKNIDEKVIAMEIAPKINAVGRMDETTNINRLIKYFVSSDTAD
                     IESFGSIIETINETRKALTKEVVESLEAIGDVPGIVLKLDIKEGLIGLIANRLVNEHQ
                     VPTIIFTEDSTDKTLLKGSMRSKEGFNITKAFGSLEKYLVTHGGHAFAGGLAIHKDDF
                     ESFKKDFIVLADRYRFVEEEIAAIPISLNDINMQNYEKIRTFSPFGEGFKEPSFLVTG
                     LKAANLTFISNDKHLSTIIGMNVKLLGFNISRQEVCKLSYIDIYGNMQLNEFRGRYTL
                     EFRVSSYRANKLL"
     gene            5835..6422
                     /locus_tag="PLH17_04230"
     CDS             5835..6422
                     /locus_tag="PLH17_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21009.1"
                     /translation="MKKKFVALIPIVALLTSCPIVNPIYPQDELDLSKYREPIFLNWA
                     NDDSEQENDILFEDGEELRSYLNTSRSVVKEVTSFINVYCGKKALRIDKGRGEIKGEL
                     IIDLKNIFRADAIALHVYPYYYSLIDRITGKTGNIYDAFSLSINERESIAINQNDEDD
                     PQMLIFCFNEPIEVITIQASTGRGFLFGLDIYQKI"
BASE COUNT         1754 a         1547 c         1467 g         1691 t
ORIGIN      
        1 gaagatgaac agtatttggt cggcttcaaa accgaatacg agaaagaagc ctttttatcg
       61 cttatctcgg ttaaaggcat cggtccgcgg accgcgcttg gcgcgatgag cgcgacgagc
      121 gccgaagaac tatttaacgc catcgccgcc aacaatatta ccttcctcaa gaagctcccc
      181 ggcatcggcg cgaaagccgc ggcccagatc atcctcgatt tgaagggtca actgactggt
      241 tctccctccg gaaatccgaa tcagtacgat gaggttcgcg atgcgctaaa aggcctcggc
      301 tataaaatta aagaaatcga aaacgtgtta gccagcatca atgaaaagga cgcgaccaac
      361 gaagatattc ttcgtttggc gcttaaaaaa ctgaaaaaat aatgagcgac agaattctcg
      421 acgctaaaaa gaataacaaa gaagaagaag ttgagatttc gcttcgcccc aaagttttaa
      481 aagaatatgt cgggcaagag gaattaaagc gcaatctgcg cgtttttatg ggtgcggcca
      541 tgcatcgcga cgagaccctt gatcatgtcc tactctatgg tcctcccggc cttggcaaga
      601 cgacgcttgc ctatgttatc gccaatgaga tgaaaggcaa tattcgcgtc atcaatggcc
      661 cctcgataga acgagccggc gatttagctt caatcttggc cgacctcaaa gccggcgata
      721 ttttattcat cgatgaaatt catcgccttc cccgcatcgt cgaggaagtc ctatatggcg
      781 ctatggaaga ttttaaactg gcaattctca tccctcgtga acagggttca aatactttaa
      841 acttaacact tgagcccttc actttgattg gagcgacgac gcgggccggc gatttgagtt
      901 cgccgctcag aagtcgcttt ggcattgtcg aaaagattaa tttctacagc gaaaaagaaa
      961 ttgaagggat tgttcgaaga acatctaagg tcctcaagac ggagattgac gagaaagcgg
     1021 tgtcgctaat cgccaagcgg agccgtggca cgccgcgcat cgctaaccgt ctctttcgaa
     1081 gagtgcgtga tttcgctaac ttcgaaggga aggacaccat cgccgaaatc gatgctaaaa
     1141 aagctctcga cgctttaaag gttgataatc ttggattaga tgatgtcgat gttcgctatc
     1201 tgagcgccat catcgagcgc tttcacggcg ggccggtcgg cttggaagcg gtggcctcgg
     1261 ccatcggcga agagattatg aacctcgagg atgtctacga gccctatctc cttcagatcg
     1321 gcatgatcaa caggacgccg cggggccgcg tggtgaccga aaaagcctat gcccatctga
     1381 aaaagaacca tcaaaaatca ctgttttaat tgtttatgcg acctcataaa acctttctaa
     1441 attaacgcgg tccgtcgcgt tttttactgg cttttgagcg aaaaattccc gttgcattac
     1501 ttttataaaa gtagtatgtt atataagcaa gcgtaaatta atcatttaat ttaaattatt
     1561 cgcttcgtaa aagtgattat ctctcacaat atcggaggac caccatgcgt cgacttattg
     1621 cttacataac tctcgccgtc tcatcgatca tcgcgatcgg cgtcggcatt aatcccatca
     1681 tgtcgaaagg catcaacaat ctcgactacc gcgatggccg tcagttcgtc tatcaactct
     1741 ttgaaaaaga cgatgaaacc atcgacgtcg acgctgaaga cgccgccaga gaagtcgcca
     1801 aagacatgga cgaaagactc aagacttggg gcgtttctaa ctacaaagtc gaaatcgaag
     1861 gcaacgatat cgtccgcgtc tcgctaaccg ctaaaaacga tactgaatat gattatttag
     1921 aaagatatct cggcttcagc ggtcaggatt tttcgattgc gaccgccgat gagtcggtgc
     1981 gtttgaccca cgatgaagtt tttaaagaca gtgaagctta tatcgtctat gaaggatatg
     2041 ctccttacgt gattattcca ttaagcaatc cgtcggagtt taaaaccctt gtcgatgccg
     2101 ccaaccaggc catgaacgat gatattgata cggtggacga tgatgagccg caatatcgca
     2161 agtcaaaagc cgaaggcgac gaagaacagc aaagcgaacc caacatctat ctatgggcga
     2221 actgggccga aggcgaagat tatgcgaaat cgcaggaaga tcaaaaggtc gccgataaga
     2281 tcatcgctca gtttttgtcg accaatatct attatgaaaa cagcgacgtt aaagaaaccg
     2341 aaatcaaata tgtcagcgga tttgccgatg aagagggaaa ttacgacacc tcgaaaattc
     2401 gccaggccaa cacccttgcc ttatttgtca aaaatctatt taacgccaat tcttatgatt
     2461 ataaagtcgt caacatcttt tccactccca tcgtcgctga agtcgaaagc atcttgacct
     2521 atgggatgtc ggttagcctc tcgatcagca aaaccctgat agccacttta gtggtcgcgg
     2581 ctgtcgtcgc cctcgtcctg gctttctttt tcagaatcgg cgccgtctcg gtaatgactt
     2641 caaccgccgt gtctttcttt gccaccttag cgatttttgt cgccttccgc gccgaattta
     2701 atatcgccgg actcctggct ttattactgg tcgccgctgc aagcctcgtg gccggaatcc
     2761 tccacttaac aaagtttaaa gaagaagttt atcgaggtcg caatttcaaa aaggccaata
     2821 ccgaagccgc gaaagcgatg accgtgccgg cgattgatat cgccgtcatc atggtcgccg
     2881 tcggcgtctt aagttatgta atcggcggtg cgctcgtcgc ttccttcggc acgatgctcg
     2941 tcattggcgg agtcgtcagt ctcgccgtca atctcatcat cttgcgcgtc atgacgtggt
     3001 tagtaacgaa cacgaccgct ttgcagggca ataataaagt ttttaatatt gaagacaaaa
     3061 atgttcctaa tctgctcaaa gaagaaaagc aaacttattt cggtccgtat caggaccgtg
     3121 attttaccaa gcgggccaaa ccggtcgcca tcggcgtcgg tgccttctcg ctgatcgcta
     3181 tcatcacgat gtctatcttt ggcgcgatta acggctcaat ctataatgct aatccagtct
     3241 acgaagaaac ctcacgcgcg tatatcaccg tctatagcga tagcccggtc gtcgacagcg
     3301 tcacatacat cgaagataat atcctgaaat tcatatacgt cgatggtgaa ctcgtctcct
     3361 atagttccat cgaccatgaa acaagggaag attatgatgc cgaaacgacg attacaacaa
     3421 cttataacta ttatatagtt aatttcgaca aagcttatag cggcgaagaa gatgcctatt
     3481 tcgaaggcga tcctttaagc gaaggcatga gccttgaagg cgttctcgat gcctacatcg
     3541 attcggtgga aggcggagaa ggcttggtcc gcatcaaagt ttcggaaaac ctcgtcatgc
     3601 agccatcgac gctgcacatt gctctcggag ctttggcggg cgtcgcttta gctgctttct
     3661 atctcgcttt ccgctaccgc atctcccgcg ctcttgccgc ctttagcgtc agcgcgatcg
     3721 cttcgtttgc gacgcttggt ttcttcgtcg tcacgcgcat tgccacgacg ccgatcgtcg
     3781 ctctggtcgt tcctgtcgtc gctttgttta gcctctttgc ctctttatat ttcttaattc
     3841 gcgagcgcga tttgctcaaa gaagaccgct caaaagagaa gaccgctcaa gtgcgcaaag
     3901 aaatcatgat taaagccacg gccttaggcg ccggaccgct ctttgtcttc ggcatcatcg
     3961 cttcctactt tgccatcaac tattttggtt ttggtccggc aaactttgcc gtcttgtttg
     4021 gtggtatgct cctcggcgtc gccctcagcg tcctcctgat cgtcaccttg cttggacctt
     4081 ccgctctctt ctttgaagag ttaattggaa aaatcgtcgc tcgcattcgc gtcccgcgcc
     4141 tgcgtaagag caagaagaga gttgccccca aatcgacttc gagcgaaccg gaagagacga
     4201 tcttcatcgg catcaacgac taatttaaag ggctactttg cgtagccttt ttaatatcga
     4261 tatggatttt ttaaacgacc ttcttaatta ttatcaacta agtaaagagc agtatgaaat
     4321 tatgacgcgg ccgctttctt atggcgattt gcgatttgac tatcgcgtaa aaggccttga
     4381 accgttgctc gtccggatta agaaggcgat tgcctcgaaa gaaaaaatta tcgtctatgg
     4441 cgattacgac tgcgatggca ttatgtcgac atcgattatc gtcaaagttt ttgagctcct
     4501 cggccatccg atcggctact atgttccgag ccgttacatc gatggctacg gtcttaatgt
     4561 taagcgtgtc gaagaaattg ccaagaaagg ctattctctg attatcacgg tcgataacgg
     4621 catcagcgct ttcgaggcga ttagcttagc caaagaaaaa ggcattgata cgctggtcgt
     4681 cgatcaccac gaagttcagg acgacaaact tcctgacgcc gtaggcattt tacatccctt
     4741 cgtctcggag ctgggcgaat acgccgccag cggcggtttt atggcctttg tcctagcaac
     4801 ggccttactc gataaggttg aaccttattt attagctttg gcgggcatct cgaccatttc
     4861 tgatatgatg ccgcttcttg gctataaccg cgacttagtg cgcctcgcca tcgaaaccat
     4921 gaacagaaac cattttccgt caatcgccag gctgaccggc gataagaata tcgatgagaa
     4981 agtcatcgcc atggaaatcg ccccgaagat taacgccgtg gggcgcatgg atgaaacgac
     5041 gaatatcaat cgcctgatta aatattttgt cagttcagac acggccgaca ttgaaagctt
     5101 cggatctatt atcgaaacga taaacgagac gcgaaaagcg ttgaccaaag aagtggtgga
     5161 atcgttagag gccatcggcg atgtgcccgg catcgtcctg aagctagata tcaaagaagg
     5221 actgatcggc ttaatcgcca accggctcgt taacgaacac caggtgccga cgattatctt
     5281 tacggaagac agcactgata aaacgcttct taagggcagt atgcgatcca aagaagggtt
     5341 taatattacg aaggcgttcg gaagtcttga gaagtatttg gtcacgcatg gcggccatgc
     5401 ctttgccggc gggctggcta tccacaaaga tgatttcgaa tcttttaaaa aggatttcat
     5461 cgttctcgct gatcgctatc gctttgtcga agaagaaata gcggccatac caatttctct
     5521 aaacgatatc aatatgcaga attatgagaa aattagaacg ttttctcctt tcggagaagg
     5581 cttcaaagag ccatcttttc tcgtcacggg actaaaagcc gcgaatttga cgttcatatc
     5641 aaacgacaag cacctgtcga caatcatcgg catgaatgtc aaactgctcg gctttaacat
     5701 ttcccgtcag gaagtttgca aattatcata cattgatata tacggaaata tgcaactaaa
     5761 cgagtttcgc ggccgttata cgctcgaatt ccgcgtctct tcatatcggg cgaataagtt
     5821 gctatgatta aaatatgaaa aagaagttcg tcgctcttat tccaatagtc gctcttttga
     5881 cttcctgccc catcgttaat cccatttatc ctcaggatga attggacttg agcaaatatc
     5941 gggaaccaat ttttttaaat tgggcaaacg acgattccga gcaagaaaac gatattctgt
     6001 ttgaagatgg cgaagaattg cgatcatatc ttaatacttc tcgctctgtc gtcaaagagg
     6061 ttacgtcatt tataaatgtc tactgcggaa aaaaggcctt gcgaatcgat aaaggccggg
     6121 gcgagatcaa aggtgaacta atcatcgatt tgaaaaatat ttttcgcgct gatgccatcg
     6181 cccttcatgt ttatccttat tactattcat tgattgatcg catcactggc aaaaccggaa
     6241 atatttacga tgccttttcc ttatcgatta acgaacgcga atcaatcgcc attaaccaaa
     6301 atgatgaaga cgatccacaa atgctgatat tttgttttaa tgaaccgatt gaagtaatta
     6361 cgattcaggc atcgactggc cgcggctttc tttttggtct tgatatctat caaaagattt
     6421 gattttaaaa aatggataaa tttataaaag aaaagcggc
//
LOCUS       DAOSSL010000042         6420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_11109_length_6420_cov_17.332129, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000042 DAOSSL010000000
VERSION     DAOSSL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11109_length_6420_cov_17.332129"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLH17_04235"
     CDS             <1..323
                     /locus_tag="PLH17_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR21010.1"
                     /translation="KEAESIARLNLAKVGLSDRILFKIPQLSGGQKQRAAIARALCMN
                     PEIMLFDEPTSALDPEMTKEVLQAIKLLASEGMTMVIVTHEMEFAHQVATRIIKVDAG
                     HVKE"
     gene            606..1670
                     /locus_tag="PLH17_04240"
     CDS             606..1670
                     /locus_tag="PLH17_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21011.1"
                     /translation="MKVGIIHTSNKKKEKRYPVHARQLTELKPEHLHKIIFEEGYPGL
                     ETLPKDLVKTAKREDIFNEVDLVILHKPVPDDYLYFREHQILWGALHCVQTPSIVDLA
                     INKKMTLIAFEQMLTPNEDGKDQSIFYRNREVAGVASVVHSLSLIGMTAGLYGNKKKV
                     AVIGYGSTGKGAIKALFGLGAEQISVYSRRSRSQIKVDDSRLAFKKYHSENGRVTMEG
                     KAPFEELSQYDIIVNCILQNPLKPIVFMTSQEALKIKKMLLIIDISCDAGMGFEFAKP
                     TSFSEPIFNVGKVVYYGVDHSPSLFYRDASYEIGKAVMPYLKYILDHDTYRGNKILEQ
                     AVDIEEGVIKNREIITFQKR"
     gene            1928..>2409
                     /locus_tag="PLH17_04245"
     CDS             1928..>2409
                     /locus_tag="PLH17_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21012.1"
                     /translation="MNNLNHLRHKHPRLFFLLILISFNVLLMIVATLIVYFAIGGYAN
                     VWDAFSTLAITWFLDPGFYSNEGNDGVKILSMIVVLTAMVSLNGGIIAFFSALLMDFL
                     DTIRSGSGKLRVSNHILILNWSSEVGEILVSYYHDTEPTTVVILSDKPRAEAEKAIKE
                     "
     assembly_gap    2410..2509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2510..3540
                     /locus_tag="PLH17_04250"
     CDS             <2510..3540
                     /locus_tag="PLH17_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21013.1"
                     /translation="VFINQTAIMGFIIAQTMVFPYLSDIYGELLGFSGADLYTEKTSE
                     PVSDYLKRHSYALPVFHKTIDGEDHLLIMADNQKDVAITRGDEDELPPLVKLKFEERQ
                     SPLTHRDVLIIGDNDKRYFIVDSLKKYTLDSGNKIHVEQKDDISEAIIQEIRQGRYSK
                     VILLAAGANSATGDPDSRAITNLLKISQAAKESETSLIIELVNPRNAQITTRYGIKYS
                     ILTNRYISRIISQTSKNPVFYPFIEDLFTYDEESSDEESYEMYIHEASELISFDEKKQ
                     YVFETPSHLVRSFYEQNPNDPYVVIGIVNEDKNDLHQYNVKLFSENLDAKVYLLVEPT
                     TKLIIVAK"
     gene            complement(3542..4024)
                     /locus_tag="PLH17_04255"
     CDS             complement(3542..4024)
                     /locus_tag="PLH17_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOR21014.1"
                     /translation="MTAERKKDLIRALKFFLFSVSAGVVQIASFTLLNEVIFVDGGAF
                     GYWFSYLVALILSVIWNFTFNRRFTFKSAANVPIAMLLVFAFYAVFAPLSTLLEWWLC
                     DLNGWNEYLVLAMNMVLNFVTEFLYQRYVVFRKSLDTNAVAEKDKAKAEHASEDSEPK
                     "
     gene            complement(4040..5632)
                     /locus_tag="PLH17_04260"
     CDS             complement(4040..5632)
                     /locus_tag="PLH17_04260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOR21015.1"
                     /translation="MKYVIAYDIGTTGVKTCLFGIDKTITLITSAHQGYGLYLLDNGG
                     AEQDVDEWWQAMVKTTAELFKKCAVLPVDIAGLSFCSQMQGLVLVDEQGKALRPAMSY
                     MDQRSKQELKEGMGGGLEFEGANLVKLLKSLMITKAASLSVKDPLWKYKWVEKNEPEV
                     FKKTHKWLDVKEYLIARATGRFIMTPDSAYSTFLYDTRKGREGWSKVLCDTFGVKMNH
                     LPDIIKATDIVGGLSKEAARDLGLIAGTPVFGGGGDATLIGIGAGAVNVGETHIYSGT
                     SGWVSTILDRQVVDIEAMIASVVALEADRFNYFAEMETAGKSLEWVKDHLALDEIGIY
                     LEKKDITCDKEKLYTSLYDYLTETITKAVPGSGGVIFTPWLHGNRCPFEDPNAAGMFF
                     NIRLETGKTELIRAVLEGICFHLRWMLECQDKKIKTSNPIRFVGGGALSKVTNQMLAD
                     ITGRTIEVVENPQNVGAVGAAAVIGVGLGIIKEIKEIKNFIPVTGIYTPNPALSVVYD
                     KNYLVFKKLYKANKLLFKELNA"
     gene            complement(5652..6308)
                     /locus_tag="PLH17_04265"
     CDS             complement(5652..6308)
                     /locus_tag="PLH17_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOR21016.1"
                     /translation="MLQKLTSEKIDAILKTGINEFAEYGLDRANINVIAKKAGISVGV
                     LYKYYKDKEAFFLACLDKSLVVLDQVISEVLIGEAKLLKRVEKIVRAVQKYSRKHVNY
                     TKMYNSITVGSNKRFAPELAKRIEGMTSQVYSQFIANAMQEGDIRQDIDPAMFAFFFD
                     SLLMMMQFSYSCEYYMERYKIFCGDGILKEDERVVQEFLKFIESAFTFDRGKIPHKAK
                     "
BASE COUNT         1694 a         1582 c         1419 g         1625 t
ORIGIN      
        1 gcaaggaagc cgagagcatc gcacgcctca acctcgcaaa agtcggtttg agcgaccgca
       61 tcttatttaa gattccgcag ctaagcggcg gccagaaaca gcgcgccgcc atcgcccgcg
      121 ccttgtgcat gaatccggaa atcatgctct ttgacgaacc gaccagcgcc cttgatccgg
      181 agatgaccaa agaagtcctc caggccatca agctcctcgc cagcgaagga atgacgatgg
      241 tcatcgtgac gcacgagatg gaattcgccc atcaagtggc gacgcgcatc atcaaagtcg
      301 atgccgggca cgtcaaagaa taatcatcat tatcgctttt ataacacggt ttctgctttt
      361 tgcgccgaag ccgtgttttt ttaataagga tgattgtggc caagaactgc aacgattaga
      421 tatgccaaaa cattcccttt tgcacattgt tcaaacttcg ccatcaaaga ccccatccta
      481 aggatgcaat cagagagtgg cctcgctttt ttgcttaaaa gcaacatcct gttataataa
      541 cttaataatc aaggatgccg cttttcgttt accgataagc cgcagatggc aaaggaggcc
      601 agtacatgaa ggtaggaata attcacacgt caaacaagaa aaaggaaaag cgttatccgg
      661 ttcacgccag gcagctgacg gagctcaagc cggagcactt gcataaaata atattcgaag
      721 aaggctatcc cggactcgag actttgccga aagatttagt caagaccgca aagcgcgaag
      781 atatctttaa tgaggtcgac ttagtcattc tccataagcc ggttcctgat gattatcttt
      841 atttccgcga acaccagatt ttatggggcg ctctccactg tgtgcaaacg ccgagcatcg
      901 tcgacctcgc catcaataaa aaaatgacgc ttatagcttt tgaacaaatg ctgacgccga
      961 acgaggacgg gaaagaccaa agcatctttt accgcaatag agaggtggcc ggcgttgcct
     1021 ctgtcgttca tagcttgtcg ctgatcggaa tgacggccgg cttatacggc aacaagaaaa
     1081 aagtcgccgt catcggctat ggatcgacag gtaagggcgc catcaaagcc ctcttcggct
     1141 tgggggcgga gcaaatcagc gtctattcgc ggcgcagccg ttcgcaaatc aaggttgatg
     1201 acagccgact agcatttaaa aaatatcatt cggaaaacgg tcgcgtgacg atggagggga
     1261 aagcgccttt tgaagaactt tcccaatatg acatcatcgt caattgcata ttgcaaaacc
     1321 ccctgaagcc aatcgtgttt atgaccagcc aggaagcctt gaaaatcaag aagatgcttt
     1381 tgataatcga tattagctgc gatgccggca tgggctttga attcgctaaa ccgacatcat
     1441 tcagtgaacc gatttttaat gttggaaagg tcgtctatta tggcgtcgat catagcccca
     1501 gcctctttta tcgggatgcc tcttatgaaa ttggcaaagc cgtaatgccg tatttgaaat
     1561 acattcttga tcacgacaca tatcgcggaa ataaaattct cgagcaagcc gtcgacattg
     1621 aggagggcgt cattaaaaac cgcgaaatca tcacctttca aaagcgttga ttttaataat
     1681 tgccttcgat atagaaggat gttcatgcaa atgcaaagag ggcatggaaa caattccgtg
     1741 cccttttata tttgattcgg cgcggaatac cgacactcgg cgagatttat gcgattatcg
     1801 agtctgacgg gccgggcaaa ttcaaaactt gtcgtttagc accacaatta ttcgcaagtc
     1861 atatatacag tcttcaagat gtagtattac aataaaacta attcgtatta tcaatagggg
     1921 cgttattatg aataacttaa accatcttcg ccacaagcat ccgcgcttat ttttcctact
     1981 tatattaatt tcatttaatg tgctgttaat gattgtggcg acattaatcg tctattttgc
     2041 tatcggagga tacgcgaatg tttgggatgc cttctcgaca ttggccatca cctggtttct
     2101 cgatcccggt ttctacagca atgaaggaaa tgacggcgtt aaaattcttt cgatgatcgt
     2161 cgtcctgacg gccatggtca gcctcaacgg cggcataatc gctttcttct cagctttgtt
     2221 gatggacttt ctcgacacga ttcgcagcgg ctccggcaag ctccgcgtca gcaaccatat
     2281 cttaattctc aactggagca gcgaagtcgg tgaaatcctt gtcagctact atcacgacac
     2341 cgaaccgacg accgtcgtca tcctttctga taagccgcgc gccgaggcgg aaaaagccat
     2401 caaagagagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgtctttatc
     2521 aatcaaaccg ccatcatggg cttcatcatc gcccagacga tggtctttcc ctacttaagc
     2581 gatatctatg gcgagctgct cggcttcagc ggcgcggatt tatacaccga aaaaacaagc
     2641 gagccggttt ccgattacct gaagcgccac tcgtatgctt tgcctgtctt tcacaagacg
     2701 attgacggcg aagaccatct cctgatcatg gccgacaatc aaaaagatgt cgccatcacg
     2761 cgaggggatg aagacgagct tccgcctttg gtcaagttaa aattcgagga gagacagtcg
     2821 cctctgaccc accgtgatgt cttgattatc ggcgataacg acaagcgtta cttcatcgtc
     2881 gacagcctca agaaatacac cctcgacagc ggcaataaaa tccacgtcga gcaaaaagat
     2941 gatatcagcg aagcgattat tcaggagatt cgccaaggcc gttacagcaa ggttattctt
     3001 ctggccgccg gagccaatag cgccacaggc gatcccgact ccagagccat caccaatctt
     3061 ttaaagattt cgcaggccgc gaaagaaagc gagacatcgt taatcatcga gttagtcaat
     3121 ccccgcaatg cccagattac gacccgatac ggcatcaagt attcgattct gacaaatcgc
     3181 tatatcagcc gcatcatctc gcagacgagc aaaaacccgg tcttctatcc gtttatcgaa
     3241 gatctcttta cttatgatga agagtcatca gatgaagaat cctatgagat gtacattcac
     3301 gaagcttccg agctcatttc ctttgacgag aaaaaacaat atgtctttga gacgccttct
     3361 catctcgtcc gttccttcta tgagcaaaac ccgaatgacc cttatgtcgt catcggcatc
     3421 gtcaacgaag acaaaaacga tctccatcag tacaacgtca aactcttcag cgaaaatctc
     3481 gacgcgaaag tctatctgct cgtcgagccg accactaagc taattatcgt cgctaaataa
     3541 attatttagg ttcactgtct tcagaagcgt gttctgcttt cgctttgtct ttttcggcga
     3601 cggcattcgt gtcaagcgac ttacggaaga cgacatatct ttgatataag aattcagtta
     3661 caaagttcaa aaccatgttc atggccaaaa ccagatattc gttccagccg tttaaatcgc
     3721 agagccacca ctcgagcaag gtcgatagcg gagcgaagac agcatagaaa gcgaagacga
     3781 ggagcatggc aatcggaaca ttggccgcgg acttaaaagt gaaacggcgg ttaaaagtga
     3841 agttccagat gaccgataag atcagggcga cgagataaga gaaccaatag ccaaaggccc
     3901 cgccatcgac aaagatcact tcattcagga gagtaaaaga cgcaatctga acgactccgg
     3961 cggataccga gaagaggaaa aacttcaaag cccgaattaa atcttttttg cgttctgcag
     4021 tcataacttg ctcctttgct tatgcattta gttctttaaa caaaagttta ttcgctttat
     4081 aaagtttctt aaagaccaag taattcttat cataaacaac gcttagcgcg ggattaggcg
     4141 tgtagatgcc ggtgacggga ataaagtttt taatctcttt gatttcctta attattccga
     4201 ggccgacgcc gatgacagcc gccgccccga ccgcgccgac attctgggga ttttcgacga
     4261 cttcaatcgt cctgcccgtg atatcggcca gcatctgatt tgtgacttta gagagagctc
     4321 cgccgccgac aaaacgaatc ggattggagg tcttaatctt cttatcctgg cattcgagca
     4381 tccaccttag atgaaagcaa attccctcta aaaccgccct tatcagttcg gtttttccgg
     4441 tttccaggcg aatattgaaa aacatacccg ccgcattggg atcttcaaac gggcagcggt
     4501 tgccgtgcag ccacggcgtg aagatgacac caccggaacc aggcacggct ttagtgattg
     4561 tctccgtcaa ataatcatat aaactcgtat acagtttttc tttgtcgcag gtgatatctt
     4621 tcttttcaag gtagatgccg atctcatcta aagcgagatg gtctttgacc cattcaaggc
     4681 tcttgccggc cgtttccatc tcggcgaaat aattaaagcg gtccgcttca agagcgacga
     4741 ctgaggcaat catcgcttcg atgtcgacga cttgtctgtc gaggatggtc gacacccacc
     4801 ccgatgtccc ggagtagatg tgcgtctcac cgacattgac ggcgcctgcg ccgatgccga
     4861 tcagcgtcgc gtcaccgcct ccgccaaaga caggtgtacc ggcgattaga cctaaatcgc
     4921 gggccgcttc tttgctcagc cctccgacga tgtcggtggc tttaataata tccggcagat
     4981 gattcatctt gacgccaaaa gtatcgcaca ggactttgct ccagccttcg cgtccttttc
     5041 tcgtatcgta aagaaaagtc gaataggccg aatcgggcgt catgataaag cggcctgtcg
     5101 cccgggcgat gagatattct ttgacatcta accacttatg cgtcttttta aacacttcgg
     5161 gttcattctt ttcgacccac ttatatttcc agagcggatc tttaacgctt aatgaggcgg
     5221 ccttggtaat catcagactt tttaacaatt taacgagatt cgcgccttcg aattccagac
     5281 ctccccccat cccttctttg agctcttgtt tggatctttg gtccatataa ctcatcgccg
     5341 ggcgtaaggc tttgccttgt tcgtcgacca agacgaggcc ctgcatctgc gagcagaagg
     5401 aaaggccggc gatatcgact ggcaagaccg cgcatttttt aaagagctcg gcggtcgtct
     5461 tgaccatcgc ctgccaccat tcgtcgacat cctgttcggc gccaccatta tcgagcaagt
     5521 aaagaccgta gccctgatgg gcgctcgtaa ttaaagtaat tgtcttatcg atgccgaaaa
     5581 ggcaggtttt gactcctgtc gtcccgatgt cgtaggcgat gacatatttc atgcgcgctt
     5641 acctcgtttc tctactttgc tttatgaggg attttaccgc ggtcaaaagt gaaggctgat
     5701 tcgataaatt ttaaaaactc ctgaacgact ctttcatctt ctttcaagat gccatcgccg
     5761 cagaagattt tatagcgttc catatagtat tcgcaactat acgaaaactg catcatcatc
     5821 agcaaactgt caaagaagaa agcaaacatc gccggatcga tatcctggcg aatatcgcct
     5881 tcctgcatcg cgttagcgat gaactggctg tacacctgcg aggtcatccc ctcgatgcgc
     5941 ttggcgagtt cgggggcgaa gcgcttgttg cttccgaccg ttatcgagtt gtacatcttc
     6001 gtataattaa cgtgcttacg actatatttt tgcaccgcgc gaacaatctt ttcgacgcgc
     6061 tttaaaagct tggcttcgcc gatcaggact tcgctgatga cctggtcgag aacgaccagg
     6121 ctcttgtcga ggcaggccag aaagaaagct tccttgtctt tataatattt ataaaggaca
     6181 ccgacgctga tgccggcttt cttggcgatg acattaatat tcgcgcggtc taaaccatat
     6241 tcagcaaact cattgattcc cgtcttgaga atcgcgtcga ttttttcgga agtcagtttt
     6301 tgtaacatgg ttatgctcct atccttttaa tttggaatat tttcgagtat agacaattct
     6361 ttgcagccag ctgtcgattt gaccgagtga acgacccttc tttgtcaagg cgacgagcat
//
LOCUS       DAOSSL010000043         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_11856_length_6100_cov_16.252109, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000043 DAOSSL010000000
VERSION     DAOSSL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11856_length_6100_cov_16.252109"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..684)
                     /gene="ssrA"
                     /locus_tag="PLH17_04270"
     tmRNA           complement(311..684)
                     /gene="ssrA"
                     /locus_tag="PLH17_04270"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(779..>1851)
                     /locus_tag="PLH17_04275"
     CDS             complement(779..>1851)
                     /locus_tag="PLH17_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR21017.1"
                     /translation="ALAQKKATDTKNLYRVTGIAQYAGYYKKGYFDLVDNGWTIVAYG
                     VSSKNTCIKLSGTTYSYTQDDSFASTGIRAGDEITLEGIFTFYSYSNSYGVPEISGYP
                     TKIISNGLSAVTAKSYTAPETYSDSYYSSINANDSGTSLGTKLHNLMMSTHKKYVSYS
                     SLTTTLKSTDSKCFYTGQSYSSFNREHVWPQAKSKDGSNQLFGESYAGADIHHIRASI
                     DTYNSYRGSTMFAPIFGTPRTVNYTGGGVVKFGSGNNYGVTEPADSRKGDVARIIMYV
                     YMHYGTDFGGTLTTGITGKLYITGVIGANSQADCFKLLRKWNAEDPVSSEEISRNETA
                     FQIQGNRNPFIDHPSFADRIWN"
     assembly_gap    1852..1951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1960..2463)
                     /locus_tag="PLH17_04280"
     CDS             complement(1960..2463)
                     /locus_tag="PLH17_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21018.1"
                     /translation="MRRRNVSAKIRLIRITKWIAVLLSIFSLATIFFPFATETLLDGT
                     VVRYPTWIAVFGGEFAFSDITQNYTINFHINIYLLVTYQLAGLSCLAFFLSNRKKTNL
                     IFALVMAFISMLAMIFMPYLVRLATPGFLVSGVQISYGPILGASTLLLTQIIALFHIL
                     TDDRFNR"
     gene            2573..3628
                     /locus_tag="PLH17_04285"
     CDS             2573..3628
                     /locus_tag="PLH17_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21019.1"
                     /translation="MKNFKLIMKSLINNDACIEGGRTKRWFFAVIFAVLSLVMAVLPI
                     MVTSLQAQGSDFTAGNYLYNYDNAIVAFSDSLKDNNLSMVVSEDANGRFLAVDKTQWE
                     AAYTANAYKQYVHLNNDSKVDFEVYYTEAVGQEFVDYYTLVSQNKSPIDGSWREAADT
                     ARSTSYLIFGRERMVGQLFQPGNTTALSGVSGDYLKLDLGFDFKSLATVEIDGVTYVT
                     SHDDKFSEPNGFLSIDAYRSQIQNRWNDVYDAVYYNTKMVTARDSTLIMLGVDALLIF
                     FMGLMIFILTRGKRNPFRIYTFWETQKIAYWASFTPSLLALIFGFIFTEYAVMIFVML
                     IGVRIMWMSMKSLKPAM"
     gene            3741..4832
                     /locus_tag="PLH17_04290"
     CDS             3741..4832
                     /locus_tag="PLH17_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21020.1"
                     /translation="MKLAKFVLLTTCAFAIAACNPSNNTSNIDPEALDLAAIDNQYAF
                     AAYTGALALQEVEVPIVQGAVRVADEESDPDEELATINRYLNLYKQLTDAGALSVVSE
                     TTSDRPDYVFQVTITTTDIDLVTTTLTLYSNEAVIDVGDDIDEDIDDSGETDYGSTNT
                     APVEDGYVYRNRNEERNIEDSTLLIGLLVTGEEEIAINGYRLVEEDFVRLGFRARLDD
                     GRVVRISHVSDATGQRFNYFIRTGRTLLVQKKMIIKVEDNKSVLKLTDTRDGITEKYS
                     FTRVELEDGLYYKIRFVTPEQRGVIHIYVSEDDLGNEVLEYYFSNGKMYYHDGTGNHA
                     GEKQGSGNGKMPDGGEDADYEEDFEDLDF"
     gene            4940..6070
                     /locus_tag="PLH17_04295"
     CDS             4940..6070
                     /locus_tag="PLH17_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21021.1"
                     /translation="MKIFKPLLVASSLFVLAACGVTPQNAVPDNEPVIDLRNTDTQYA
                     FATYTGVRMLEEVSLASAPQVLGRVDEDITPDEELAIVNNYLTLYKELTEEGVLNVAM
                     AETSDREEYAFQLTITTTDVDKIQSTFTLYFNEVVIEDEDDIGEGIDDTGEDVLEDTP
                     EGEQNGENYRHREDPRSDDESTLLVGLAVIGDEEFEVNGYRLVEEDEVRFGFWARLDD
                     GRVLRISHHAEEDVQKFQYFLRNGRDLIARKKVMIEVEDNQTMLKLEDVKDNVRSRYS
                     FKQEVGEDGTYYKIRFDTPEGKGVIHIYVTVDELGQEIIEYYFSGGNKYHHDGNGNHH
                     GEHDDDDDRGNGNGGNGNGNRDCPEDDDEDEYEEDFEDLDDF"
BASE COUNT         1676 a         1335 c         1423 g         1566 t
ORIGIN      
        1 ggccggccgc caaaaatgta acggatttga aaaataatta atgagtcggc taatcgttca
       61 tagaatattt atcaaactaa gagttaaaaa atagcggagg atggagcgta ttagttaacc
      121 tattttcttg atcgtgttat aggttaactc tcggaatggt ctggcgttta ttgtttcatt
      181 acttaaaccg caatctaatt ttcggcagaa gcaaatatcc aattatatag agaaaaaatt
      241 ttcttacaaa aatgctccct tttaattgca ccactttgtg gtccaaattt agggagcatg
      301 ttcaactgat tggtggagat gcggagaatc gaactccggt ccaaagaagg tcccacaata
      361 acgtctacag cttagacgca atttgttttt aacaccagat gacaataacg tcgaagtatc
      421 tgacgcgaga ctaataggtt ttcgtattct tcagctagtc aacttggaat cttattccct
      481 tgttatgaca cttgcccgaa gcgtgaggga cgaaaccttc ggaagcgcgc tgcagggtca
      541 gagacccaga ccagtattta ggtgcttaat taagcggcag cgaatgaaag atttctgttg
      601 ccagttatct ttggtttgat gattacgtca tcactcggct gcagttactg cgaacacaac
      661 cctgtcgatg ccatgaacat ccccgtgttg gcttatatta tctataagcg ttgttatctt
      721 aacacaatca aaaaacaacg acaacaaaac gacatgatat atacctatta aaatatgatt
      781 aattccagat gcgatcggcg aaggatggat ggtcgataaa cgggttgcga ttgccctgta
      841 tttggaaggc cgtttcgttg cggcttatct cttcactgct gacaggatcc tcggcattcc
      901 acttacgaag taatttaaag caatcagcct ggctgttcgc cccgatgacg ccggtgatat
      961 ataacttgcc ggtgattccg gtcgttaaag ttccgccaaa atcggtgccg taatgcatgt
     1021 agacatacat gatgatgcgg gcgacatcgc ctttgcgcga gtcggcgggt tcggtcacgc
     1081 cgtaattgtt acccgagcca aatttaacga ctccgcctcc ggtgtaattt actgttcggg
     1141 gcgtgccgaa tatcggagcg aacatcgtgc tgccgcgata ggaattatag gtgtctatcg
     1201 aggcgcgaat atgatgaata tccgctccgg catatgattc gccaaacagc tggttgctgc
     1261 cgtctttgga cttcgcctgc ggccaaacat gctcgcggtt aaaagagctg taactttgtc
     1321 cggtgtaaaa gcacttgctg tccgtggatt tcaaggttgt agtgaggctg gaatacgaaa
     1381 catatttttt gtgtgtggac atcatcagat tatgaagttt tgtacctaat gatgtgccgc
     1441 tatcattcgc atttattgaa ctataatatg agtctgagta tgtttcgggg gcggtatagg
     1501 acttcgcggt tacagcgctt aatccattgc tgataatttt ggtggggtag ccagatattt
     1561 ctggcacacc gtaactattt gagtaggaat aaaaagtgaa aatcccttct aaggtaattt
     1621 cgtctccggc ccggataccg gtgctcgcaa aagaatcgtc ttgggtataa gaatatgtgg
     1681 ttccggatag tttaatacag gtgtttttgc tgctgacgcc ataggcgaca atcgtccagc
     1741 cgttatcgac gaggtcaaaa tatccttttt tgtaatagcc ggcatattga gcgatgccgg
     1801 tgacgcgata gagatttttg gtatcggtgg cctttttctg agccaaagcc tnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatcaaaat taacggttga agcggtcgtc
     1981 ggtcagaatg tgaaagaggg cgatgatctg agttaggagc aaggtgctcg cgccgaggat
     2041 gggcccatag cttatctgaa ctcccgagac gagaaatccc ggtgtggcca ggcgcaccaa
     2101 ataaggcatg aaaatcatcg cgagcatgct gatgaaggcc atgacgagag cgaagatcag
     2161 atttgtcttt ttgcgattgg ataaaaagaa ggctaaacag cttaagcccg cgagttggta
     2221 tgtaactaaa agatagatgt taatatgaaa attaatcgtg tagttttggg ttatgtcgct
     2281 aaaagcgaac tcgccgccga agacggcaat ccatgtcgga tagcgcacga ccgttccgtc
     2341 taaaagcgtc tcggtggcaa aaggaaagaa gattgtcgca agcgaaaaga tagacagcaa
     2401 aaccgcaatc cacttcgtaa ttctgatcag acgtattttt gcgctcacgt ttcttctccg
     2461 cataatatag tttataccaa ttcatttagc aacatcttcc attgtttaat agtgtttgaa
     2521 aatgaacctt atatataata aactatgtag gcgattggaa ttattgataa atatgaaaaa
     2581 ctttaaactt atcatgaaga gcctcatcaa caatgatgct tgtatagaag gcggccgtac
     2641 caagagatgg ttttttgccg tcatttttgc ggttttatcc ctcgtcatgg cggtccttcc
     2701 catcatggtg acctcccttc aggctcaggg ctcagacttc acagctggca attaccttta
     2761 taattacgat aacgcgattg tcgcttttag cgattcgctc aaagacaata atctctcgat
     2821 ggtcgtaagc gaagacgcaa acggccgctt tttagcagtt gacaaaacgc agtgggaagc
     2881 cgcctatacc gctaacgcct ataagcaata tgtccatctt aacaacgaca gcaaagttga
     2941 ttttgaagtt tattacaccg aggcggtcgg tcaggaattc gtcgattatt acactctcgt
     3001 ctcgcaaaac aagagtccaa ttgatggctc ctggcgcgaa gcagccgata ccgcccgcag
     3061 cacaagttat ttaatcttcg gacgcgagag aatggtcgga cagctctttc agcccggcaa
     3121 caccaccgcc ctctccggcg tctccggcga ttatctaaaa cttgatttgg gctttgattt
     3181 taaatcgctg gccaccgtcg aaatcgatgg cgtcacttat gtgaccagcc atgacgataa
     3241 attcagcgag ccaaacggtt ttcttagcat cgacgcctac cgcagccaaa ttcagaatag
     3301 atggaacgat gtttatgatg ccgtctacta taacacgaag atggtgaccg cccgcgattc
     3361 aactttgatc atgctcggcg tcgacgcctt gctgatcttc ttcatgggtt taatgatttt
     3421 tatcctgacc cgtggcaagc gcaatccctt ccgcatctat actttctggg agacgcagaa
     3481 gatcgcctac tgggcctcct tcacgccttc cttgctcgcc ttgatcttcg gttttatctt
     3541 caccgaatac gcggtgatga tcttcgtcat gctaatcggt gtccgcatca tgtggatgtc
     3601 gatgaaatcg ctgaagccgg cgatgtaaat tgctgggtaa tgcaaaaaaa taaggagagt
     3661 ttgctctcct tttttggtgt ctgaatatct actcactaaa atgacataat cgtttgttta
     3721 tattttagga ggtagagaat atgaaactcg ccaaattcgt gcttttaaca acatgcgcat
     3781 ttgcgatagc tgcttgtaat ccgagcaaca atacttccaa cattgatccc gaggccctcg
     3841 atttggctgc aatcgataat cagtatgctt ttgctgctta taccggggct ctcgctcttc
     3901 aggaggttga ggttcccatc gttcaaggag ccgtaagggt tgccgatgaa gaatctgatc
     3961 ccgatgagga gctggcgacc atcaaccgct atttaaatct ctataaacaa ctgactgatg
     4021 caggggccct aagcgttgtc agcgaaacga ctagcgatcg tcctgactat gttttccaag
     4081 tcacgatcac gaccaccgac atcgatttag tgaccacgac attaactttg tattccaatg
     4141 aagcggtgat tgacgtcgga gatgacattg atgaagacat cgatgatagc ggcgagaccg
     4201 actacggaag cacgaatacc gctcccgttg aagatggtta tgtctaccgc aatcgaaacg
     4261 aagagcgcaa catcgaagat tctactctgc tgatcggctt attagtgacc ggtgaagaag
     4321 aaatcgcaat caacggctat cgcctggttg aggaagattt tgtccgcctc ggtttcagag
     4381 cccggctcga tgacggaaga gtcgtccgca tctcgcatgt cagcgacgcg accggccagc
     4441 gcttcaatta ttttatccgc accggacgca cgttgctcgt tcagaaaaag atgatcatta
     4501 aagtcgaaga caacaagtcg gtcttgaagc ttaccgatac gcgcgacggc attaccgaaa
     4561 agtattcgtt cacccgcgtc gaactcgaag atggtctcta ttataaaatc cgctttgtga
     4621 caccggagca aagaggcgtc atccatattt atgtcagcga agatgactta ggtaatgagg
     4681 tgctcgaata ctatttctcg aacggcaaga tgtattatca cgacggaaca ggaaatcacg
     4741 ccggagaaaa acaaggtagc ggcaacggaa agatgcctga cggcggagaa gatgctgatt
     4801 acgaagaaga ttttgaagac ttagatttct agttaatctt tgattaatag agaagggaga
     4861 tcggttctcc cttttctttt taaaaaatat aatcattaaa aactactatc tttttattta
     4921 tatggtagag gacatattta tgaaaatatt caaacccttg ctcgttgctt ccagcctttt
     4981 tgtgctcgcc gcctgtggcg tcacaccaca aaacgcggtt cccgataatg aaccggtcat
     5041 cgatttaaga aacactgaca cacagtatgc ctttgccacc tacaccggcg ttcgcatgct
     5101 cgaagaagta agcttggcaa gcgctcctca agttttggga agagtggatg aagacatcac
     5161 tcccgacgaa gagctcgcca tcgtcaataa ctacctcacg ctttacaagg aattgacgga
     5221 ggaaggcgtc ttaaatgtcg cgatggccga aacaagcgac cgcgaagaat acgctttcca
     5281 attgacgatt acgacgactg atgtcgataa gatacagtcg acattcaccc tttatttcaa
     5341 tgaagttgtc atcgaagacg aagatgatat cggcgaaggc atcgatgata ccggcgaaga
     5401 tgtcctcgaa gatactccgg aaggcgaaca aaacggtgag aattaccgcc accgcgaaga
     5461 tccccgcagc gacgatgaat ccacccttct agtaggtttg gccgttatcg gggatgaaga
     5521 attcgaagtc aatggttacc gcctggtcga agaagacgaa gtccgttttg gtttctgggc
     5581 ccgcctcgac gacggccgcg tgcttcgcat ctcccaccac gccgaggaag atgtgcaaaa
     5641 attccaatat ttcttaagga acggacgcga tttaatcgct cgtaaaaagg tgatgatcga
     5701 agtcgaagac aatcagacga tgcttaaact cgaagatgta aaagataatg tccgctcccg
     5761 ctattcgttc aaacaggaag tcggcgaaga cggcacctac tataagattc gttttgatac
     5821 gccagaaggc aagggcgtga ttcacatcta cgtcaccgtc gatgagttgg gccaggaaat
     5881 tatcgaatac tatttctccg gaggtaataa atatcaccac gacggaaacg gcaaccatca
     5941 cggcgaacac gatgatgacg atgaccgcgg aaacggaaac ggcggcaacg gaaacggcaa
     6001 tcgcgattgt ccggaagatg atgatgaaga cgaatacgaa gaagactttg aagacctaga
     6061 cgatttctaa agatgacata ataaaaaccg agggtcgaat
//
LOCUS       DAOSSL010000044         5864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_12448_length_5864_cov_15.413841, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000044 DAOSSL010000000
VERSION     DAOSSL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12448_length_5864_cov_15.413841"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..571)
                     /locus_tag="PLH17_04300"
     CDS             complement(161..571)
                     /locus_tag="PLH17_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOR21022.1"
                     /translation="MRNKVLIIGCGRLGANIAALASEKGQNVIVVDRNHSAFDRLPES
                     FSGYRVTGDATDLSLLEEAYIKTAKEVVIATGDDNVNLFLAHVACLIYEVPSIYVRLD
                     DPERGALLNGLKVKAIYPFELSINKFNLMKGEDL"
     gene            746..>1515
                     /locus_tag="PLH17_04305"
     CDS             746..>1515
                     /locus_tag="PLH17_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOR21023.1"
                     /translation="MKKYLKLIKEALEGIEKLYIFHFFVLLVSVFFMILSSFTSKILI
                     DTLRGDIFDNPKAGALEIFIANIYGGPQFLSENLWFFAIIIVTIAIAVALLVFSRIFI
                     RSYLGPRIARNMQLKLFNHIERLPYSFMKKSQSGDVIQTCTRDEEVFRRFMTRETYSI
                     AYTFFIVAFSFSILFSINWQLALVSMTLMPLLFIYSFFVIKEVRVRYRKTDDSEALLT
                     SKIEENLSSVRVVKAYNNEAYEIDSFEKRLVDYRKKYI"
     assembly_gap    1516..1615
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1616..2338
                     /locus_tag="PLH17_04310"
     CDS             <1616..2338
                     /locus_tag="PLH17_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOR21024.1"
                     /translation="IFENVAFKFDDADKPVLQNVNLKITKGATVAVLGKTGAGKSTLA
                     YLLTRLYDPTSGRILIDGQDITGIARAHIRQNVATVLQEPFLFSKSIISNIRIANPNA
                     SEEEVYKAARIADIHDNILTFKQGYETPVGEKGVTLSGGQRQRLAIARTIINKAPILI
                     FDDSLSAVDTETDINIRTALKKRSEETTTFIITHRIATAKDADLIVVLEDGTISEQGT
                     HEQLIKKKGLYQRVYEIQTKLQ"
     gene            2340..4280
                     /locus_tag="PLH17_04315"
     CDS             2340..4280
                     /locus_tag="PLH17_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOR21025.1"
                     /translation="MANFAFEEEKAPSKFNLGVWRKMLRYTFRNWPLLIVLALSMLLT
                     TFYDSSFMPLMNAAAIAAIPEIPLGQSVAGMIINVELIFGIRFTLDFVGFALVFGAGI
                     LIRSISIFVTFFVTNYFDMLINTSLRRDAFRRAQELSFSYYDRTPSGWLIARMQNDTS
                     AISDTLSWGLIRIVWITFELLFTLITMFSRDVRLSLIILSIAPLLMIVAPIFQRLLLK
                     AQRKARSAYSNFVRWLVECINGVKTIKTLNIEEKMYEEADDVTMNIYVKRRKAFKIHA
                     FFMPVINILSAATTALVILLATANFGLLDAMTPAGVATLVLFIGFVSQIYNPIQEFSE
                     VANEFIETQAKVEKVMSLIETKPEIVDTPEVVAKYGTLFDNKKENFEPLNGDLVFEHV
                     SFSYVKDTEVIHDLNLHIKQGTSLAIVGETGSGKSTTANLLCRFYEPSSGRILVDGID
                     YKERSVGWLRSNIGYVQQTPFVFRASFYDNVRYGRPEASLEEVIEACKVVGVHDFIMA
                     QKDGYQTELDDGGSQLSGGQKQLISFARAIIRNPRLLILDEATSSIDTESEGVIQSAM
                     NKILQGRTSIIIAHRLSTIVNCDRILVMSEGKIIEDGSHCELMSRKGHYHHLYMNQFR
                     ELNLESQIATYEEQIEKMDVKL"
     gene            complement(4316..5788)
                     /locus_tag="PLH17_04320"
     CDS             complement(4316..5788)
                     /locus_tag="PLH17_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOR21026.1"
                     /translation="MSDTLLTIAIACLAVLLILVCVVLIIILKKRKSVDGGVSNEEVR
                     KLLEDHRVSTLKDFNDGVAKQNLAINTLKEAIANGMGDAQKTNQKDLYTFLAETKTKL
                     VELQANFNKESADVKQQNIGNIKDLMAQTTKDVNELKAKILAEINDVNTKNNQNINDQ
                     NVQTQKRISDQIQTLKDQVAKSLEQGFEKNDKAIQEFIAKTAAIESSTRQIEDLRKEI
                     AKFNNILSNQKTRGNFGEDVLENIFLAVFGENAAGKFYRTQVNLSKEFGVKQTKEEGG
                     EKSDVIVDFVYDLATDHGILPLSIDAKFPYSNYLPLLDESITPEAREEARKKFKTDVK
                     TRIKEVSKYIIDGKTAPYAVMFVPAEAVFLDIFKEFPDVVEEARKQKIIIASPSLIIT
                     IIQILKFVLEDYQRRNEAEERLAMIVELENQFRLFSDRWKIHRAHVLALTEDIKKIDT
                     TSQKIIRDFDSAKSLLDENPLKKIAEGDQVIDVDVQNDDE"
BASE COUNT         1504 a         1343 c         1200 g         1717 t
ORIGIN      
        1 ttatacggtt cgccgacaaa gacggccact ttattgtttt taacgatata atcagcaaag
       61 ttcttatcgg agtttattac aattaaatct ttcgtctgat ctttaaacat gttaatgacg
      121 aagtcggcct cgtgtttgcc gttggcgatg acgattttca ttataaatcc tctcccttca
      181 tcaaattaaa cttattaatt gatagttcaa acggatagat ggctttgact tttaaaccgt
      241 tgagaagggc gccgcgctcg gggtcatcga ggcgaacata gatacttgga acttcataga
      301 tgaggcaagc gacatgggca agaaagagat taacattatc atcaccggtg gcgatgacga
      361 cttccttggc ggttttaatg taagcttctt caagcagcga taaatcggtt gcatcacctg
      421 tgactcgata gccgctgaaa gattcaggaa ggcgatcgaa ggcactgtga ttgcggtcga
      481 caacgatgac gttttgacct ttttcggagg ctaaagcggc aatgttggcg cctaatcttc
      541 cacagccgat aatcaacact ttattgcgca tatagtttgg acctcgtgtt cacagtcatt
      601 ttttatgaac ataatgtatt atacaaccat catcaagtaa atagaatgta ggatttatga
      661 tgaggcatca ccaatttgct ctttcgtctt tttcttttaa agaaaataat atacaatatc
      721 tccttgtctg atgattgact aaactatgaa aaaatacctg aagttaatta aagaagccct
      781 cgaaggaatt gaaaagctct atatcttcca tttctttgtt ttgctcgtct cggtcttttt
      841 catgattttg tccagtttta caagcaaaat cttaatcgac acccttcgcg gcgacatttt
      901 cgataacccc aaagccggcg ccttagaaat ctttattgcc aatatttatg gcggaccgca
      961 atttctgagc gagaatctgt ggttttttgc tatcatcatc gtcacgattg ccatcgctgt
     1021 cgccctcctt gttttctcgc ggatttttat tcgctcttac cttggtccgc gcatcgcccg
     1081 caatatgcaa cttaaacttt ttaaccacat cgaacgcttg ccgtattcat ttatgaagaa
     1141 atcgcagagc ggagatgtca ttcaaacttg cacacgcgat gaagaagtat ttcgccgttt
     1201 catgacgcgc gaaacttatt cgatcgccta cacctttttt atcgtcgctt tctctttttc
     1261 catcctcttt tccatcaact ggcagctcgc tctcgtctcg atgacgctca tgccccttct
     1321 ttttatctat tcctttttcg tcattaaaga agtgcgggtc cgctaccgga aaacagatga
     1381 ttccgaggct ttgctcactt ccaaaatcga ggagaattta tcttcagtcc gagttgtcaa
     1441 agcctacaac aatgaagcct atgaaatcga tagttttgaa aagcgccttg tcgattatcg
     1501 caaaaagtac attcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatttt
     1621 tgaaaacgtc gcttttaagt ttgatgacgc cgataaaccg gtgctacaaa atgttaatct
     1681 gaaaatcaca aaaggcgcga ccgtcgccgt cctcggcaaa acgggagcgg gcaaatcgac
     1741 actcgcctac cttctgaccc gcctttatga tccgacttcc ggccgcattt taatcgatgg
     1801 ccaagacatc acaggcatcg cccgtgccca cattcgccaa aatgtggcca ccgtccttca
     1861 ggagccattt ctcttttcaa agtcgatcat cagcaatatc cgcatcgcca atcccaacgc
     1921 cagcgaagag gaagtgtata aagctgcgcg aatcgccgat attcacgata atatcttaac
     1981 ttttaaacaa ggctatgaaa ctccggtcgg agaaaaagga gtcacgctct ccggcggtca
     2041 aagacagcgt ttggcaattg ccagaacaat cattaacaag gccccgattt tgatttttga
     2101 tgattctttg agcgcggtcg ataccgagac cgatatcaat attcgcacgg ccctcaagaa
     2161 gcgctcagaa gagacgacga cttttattat cacgcaccgc atcgcgacgg ctaaagatgc
     2221 cgatttaatc gtcgtccttg aagacggcac catcagcgag caaggcactc acgaacaact
     2281 aattaagaag aaaggcttgt atcaacgcgt ttacgagata cagacaaagt tacagtaaaa
     2341 tggctaattt tgcatttgaa gaagaaaaag caccgagcaa gtttaatctc ggcgtgtggc
     2401 gcaaaatgct gcgttatact tttcgcaatt ggccgctttt aatcgtcctc gccctttcga
     2461 tgcttcttac aactttttac gactcgagtt ttatgccatt gatgaatgcc gcggccatcg
     2521 cggcgattcc cgagattccg ctcgggcaaa gcgtcgccgg catgattatc aatgtcgaac
     2581 ttatattcgg cattcgtttc acccttgatt tcgtcggctt tgcccttgtt ttcggagccg
     2641 gcatcctgat tcgctcaatt tccattttcg ttactttttt tgtcacgaat tactttgata
     2701 tgctaattaa cacttcgctg cggcgcgatg ctttccgccg agcccaggaa ctaagtttct
     2761 cctactatga ccgcacacct tccggatggc tcatcgcccg catgcaaaat gatacatcag
     2821 ccatcagcga caccttgtcg tgggggctca tccgcatcgt ttggattact tttgagctgc
     2881 ttttcacttt gataacgatg ttttcgcgcg atgtcagatt atcgctcatc atcttatcaa
     2941 tcgcaccatt attgatgatt gtcgcgccca tctttcagcg cttattatta aaggcgcagc
     3001 gcaaagcccg aagcgcttat tcaaattttg tccgctggct cgtcgaatgc atcaatggag
     3061 tgaaaaccat caagacgctt aatattgaag aaaagatgta cgaagaggct gatgatgtga
     3121 cgatgaatat atatgtcaag cggcgcaagg catttaaaat tcatgctttc ttcatgccgg
     3181 ttatcaacat tttatcagct gccaccaccg ctctcgtcat cctgctggcc accgctaatt
     3241 ttggcttgct cgatgcaatg acgcccgccg gtgttgcaac cctcgtcttg tttattggct
     3301 ttgtctcaca aatttataat ccaattcagg agttttccga agtcgccaac gaattcatcg
     3361 agacgcaggc caaggtcgaa aaagtcatgt cgctcatcga gaccaaaccg gaaatcgtcg
     3421 acacacctga agtcgttgca aaatatggca ctctttttga taataaaaaa gaaaactttg
     3481 agccactgaa cggcgatctc gtctttgaac acgtcagttt ctcatatgtt aaagacacgg
     3541 aagtaattca cgatttaaat cttcacatca agcagggaac ttcgctagcc atcgtcggtg
     3601 agacgggttc cggaaagtcg acgactgcta atctcctctg ccgtttttat gaaccgagct
     3661 cgggtcgcat tctcgtcgat ggcatcgatt ataaagagcg ttcggtcggg tggctaagaa
     3721 gtaatatcgg ctatgtccag cagacgccgt ttgtctttcg cgcctccttt tatgataatg
     3781 tccgctacgg tcggcctgag gcctcactag aggaagtaat cgaggcgtgt aaggttgtcg
     3841 gtgtccacga ttttatcatg gcgcaaaaag atggctatca aaccgagctc gatgacggcg
     3901 gaagccagct atcgggcggc cagaaacaac tcatctcctt tgcccgtgcc atcatccgca
     3961 atccccgcct tctcatcctt gacgaggcga catcaagtat cgacacggaa agcgaaggcg
     4021 tcattcagtc ggctatgaat aaaattcttc aaggtcggac gagcatcatc atcgctcacc
     4081 gcctctcgac aatcgtcaac tgcgaccgca ttctcgtgat gtccgaagga aagattattg
     4141 aagacggcag ccactgcgaa ctcatgagtc gcaaaggaca ctatcatcat ctatatatga
     4201 atcagttccg cgagctgaat ctcgaatcac aaatagcgac ttacgaggaa caaatcgaga
     4261 aaatggatgt taaattataa aaacaggccc caaatcatcg ggagcctgtt ttttattatt
     4321 cgtcgtcgtt ttggacgtcc acatcaatca cttgatcgcc ctcggctatc ttcttaagcg
     4381 ggttttcatc gagtaagctc tttgctgaat caaaatcgcg aataattttt tgcgaagtgg
     4441 tatcaatttt tttaatgtcc tcagtcaaag ccaacacatg ggcgcgatgg attttccagc
     4501 gatcgctgaa gaggcgaaac tgattttcga gttcgacgat catcgccaat ctttcttcgg
     4561 cttcatttcg cctttgataa tcttctaaaa cgaattttaa gatttgaatg atggtaataa
     4621 tcagacttgg cgaagcgata atgatttttt gtttgcgggc ttcctcgacg acatcaggaa
     4681 attccttgaa aatatcgaga aagacagctt cggcaggaac aaacatgacg gcatacggag
     4741 cggttttgcc gtcgatgata tatttagata cttcctttat tctcgtctta acatcggttt
     4801 taaatttttt gcgcgcttct tcgcgggctt ccggagtgat actttcatca aggaggggaa
     4861 ggtagtttga ataaggaaac ttagcgtcga tagaaagtgg cagaatgccg tgatcggtcg
     4921 ccaagtcata gacaaaatca acgattacat cacttttttc tccgccttct tctttcgttt
     4981 gcttgactcc gaactccttg ctcagattaa cttgggtacg atagaacttg ccggctgcat
     5041 tctcgccaaa aacagccagg aaaatgttct ctaaaacatc ttctccaaag ttgccgcgcg
     5101 ttttttgatt gctcaaaata ttattgaatt tggcaatctc tttgcgaaga tcttcgattt
     5161 ggcgggtcga actttcaatc gccgccgtct tagcaataaa ttcttgaata gccttatcat
     5221 ttttttcaaa gccttgttcg agtgattttg ccacctggtc tttgagtgtt tgaatctggt
     5281 cgctgatgcg cttttgcgtc tgaacatttt gatcgttgat gttctgattg tttttggtat
     5341 tgacatcatt tatttcggcg agaattttgg cttttaattc attgacgtct ttcgtcgtct
     5401 gagccatcaa atctttgata ttgccaatat tttgctgttt gacatcagcg gattccttat
     5461 tgaaattagc ttgcagttcg acgagctttg tctttgtttc cgctaaaaat gtatacaaat
     5521 ctttttgatt tgttttttgg gcatcgccca tcccgttggc gatggcttct tttaaagtgt
     5581 taattgccag gttttgtttg gcgactccgt cattaaaatc tttaagggtg ctgacgcggt
     5641 gatcttcaag gagcttgcgg acttcttcgt ttgagacacc gccatcgaca ctttttcttt
     5701 ttttcaagat gataattaag acgacacata ccagaattaa taaaaccgct aaacaagcga
     5761 tggcgattgt caataaagta tctgacattt gaaatcctcc atagttctta caatacatta
     5821 tgattataca ataattgacg ctgctatctg caggttaaac gaca
//
LOCUS       DAOSSL010000045         5251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_14369_length_5251_cov_13.040801, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000045 DAOSSL010000000
VERSION     DAOSSL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14369_length_5251_cov_13.040801"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1412)
                     /locus_tag="PLH17_04325"
     CDS             complement(132..1412)
                     /locus_tag="PLH17_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOR21027.1"
                     /translation="MRILFEDARILSMNDKPLFRGDLVVDGNRIVYLGHNGSRQGPFD
                     RVILCDGNVLMPGFKNAHTHSAMTFLRSYADDLALRDWLYNWVFPIEAKLQPSDVYHL
                     SKLAFAEYLTSGITANFDMYYFPKEIARASMDFGMRTLILGTPTSFKESIEGLRENYE
                     AINGRSPLVNFRLGFHAEYTSTHEMLVSLAKLANELKAPVFTHISETQNEVNGCIERH
                     GDTPAVYFEKLGLFNYGGGGFHGVYLTDQDMEIFKRRNLHLVSCPSSNTKLASGIAPI
                     QKFYDYGINLSLGTDGPASNNCLDFFREMFLATGLSKLKTMDPTSLDAYKVLKMATVG
                     GAKAMGLDEADTLEVGKLADIIMIDLKRPSMQPLNNIEKNIVYSGAKDVIKMTMIDGR
                     ILYEDGKFFLGEPIEDLYKRAQEVTDRIKQEITK"
     gene            complement(1454..1576)
                     /locus_tag="PLH17_04330"
     CDS             complement(1454..1576)
                     /locus_tag="PLH17_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOR21028.1"
                     /translation="MQKYRCLRCGQIVVPNEDGTCPVCGAPKEKLRPLPEPKKQ"
     gene            complement(1650..3248)
                     /locus_tag="PLH17_04335"
     CDS             complement(1650..3248)
                     /locus_tag="PLH17_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOR21029.1"
                     /translation="MKKNTTLYGALLATSTRYPKRNALIYMRQKITYEGLLRDVNRLA
                     KGLADYGVKTDDVVSVCLPNIPSAVHLLYAINQLGAIANLIHPLMKYEQMKGIMEETK
                     SKLLFVLDSFFAEFTDLTKLGIRVVACSPVQTLNPLVKTIYSLQNRSKIKNIANSDKI
                     LSFYQKEGVFACDNDFTKDAIYLHSGGTTGKPKVVALSSFSINALASNGLDILGINDT
                     KDKFMLAVLPIFHGFGLCMGMHACLAFGACNTLMPKFHSEQVVKYLKKKQISFIIGVP
                     ILFEALLRNKHFRGPALKNLLICFVGGDFVAPSLFERFNNLMEKYGSSARLYEGYGLT
                     EVVTVCSVNNHRFHKAGTVGKILPNVDMKVVDLDTKSDMPFGRDGELYVRGETMMNGY
                     RFLQDEKDQPFVRDKDGQVWIATGDYGSLDEDGFVYFRQRLKRIIKVSGINVFPNEIE
                     GAVMDMGSVHECAAVAIADEKLGNAIKLFVVLDHAAKASNFDDEIKAVVLKRCGVYAV
                     PKEIVYRDHLPKTLVGKVDTKALS"
     gene            complement(3259..4242)
                     /locus_tag="PLH17_04340"
     CDS             complement(3259..4242)
                     /locus_tag="PLH17_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOR21030.1"
                     /translation="METALIDGYLLHLPLRTMKKKAKRIFEIDLLRGLLIIMMVVDHL
                     AYDFGQLAPSFFLVSDAPQALRDLIEWCYNYWYEAWRINFRYAVIALFFILSGLSTYL
                     SRNSIKRGMVVLGFGAIISIFSYFLSITLKTDLFIFFGIILCLGLAMITYSLFRTLII
                     KMTKSKTFWLWTSLFLAILFTGVGYWMRLEVATVPVDAQNWWFIINGRMTPTINSYRI
                     IQGVFTPIVYGLDVKLDIVLGRLWYGVDWAGLFPYLGYTFFGGFLGELLYAQKKSLIF
                     KRENVVWHPVEKVFRPLTFIGSKTIYIYLLHQVVLAALTALIFILLGIPLK"
     gene            complement(4307..5203)
                     /locus_tag="PLH17_04345"
     CDS             complement(4307..5203)
                     /locus_tag="PLH17_04345"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HOR21031.1"
                     /translation="MRILFLLFSGTNNTLEIARLLVSDFALKGHVSEIVNINIDTPLI
                     DISNYDLVGLGYPIYAFNEPRFFWRYVKRLQLCKNVKYFIFKSSGETLALNNASSRRI
                     IRKLRKEKCDVLGDYHFVMPYNIHFRFDDAFVKEIMIYNRKLSKILVENVIKGKKSFL
                     KTNILYNFLAWIIGIQRPGATINSYLYRTDMNKCIKCMKCVRDCPTHNIYYEDGKIKF
                     RHSCQMCMRCSFFCPVDAISIGYINAWRVNGAYRFFEIEKNEALPLPYITRQSKGFYR
                     CFIRYFANIDKAYSEYISGHNE"
BASE COUNT         1487 a         1158 c         1224 g         1382 t
ORIGIN      
        1 gcaaaggcgg tcgatgagcc gacggcgccg tctccgatga tgacaacttt gcggttttcc
       61 atagtaaata acctccgcat aaatattaaa atatttggag gtcatttcat aacgatatga
      121 ttataaatag gctatttcgt aatctcttgt ttaatgcggt cagtaacttc ctgggcccgc
      181 ttgtagagat cttcaatcgg ttcgccgagg aagaacttac catcttcgta taaaatacgg
      241 ccatcgatca tcgtcatctt aatgacatct tttgcgccgc tgtagacgat atttttctcg
      301 atgttattga gcggctgcat gctcgggcgc ttgaggtcga tcatgataat gtcggcgagt
      361 ttgccaactt cgagggtatc cgcctcatct aagcccatcg ccttggctcc gccgacggtc
      421 gccatcttca agactttata agcatcgaga gaagtcggat ccatcgtctt aagcttggaa
      481 agaccggttg ccaaaaacat ctcgcggaag aagtcgaggc agttgttgct ggccggaccg
      541 tcagtgccta acgaaaggtt gattccgtag tcataaaact tctgaatggg ggcgatgccg
      601 ctggccagct tcgtgttgct gctcgggcag gagacgagat ggagattgcg gcgcttaaat
      661 atctccatgt cctgatcagt caaatagacg ccgtgaaaac cgcctccgcc atagttaaat
      721 aaacctaatt tctcaaagta gacggccggt gtgtcaccat gtctttcaat gcagccgttg
      781 acttcatttt gcgtctcgct gatgtgtgtg aagacgggcg ctttgagttc gttagctaat
      841 ttagctaaag atacgagcat ctcgtgagtt gaggtatatt cggcatgaaa accgagccgg
      901 aaattgacga gcggcgagcg gccgttgatc gcctcgtaat tctcacgcaa accttcgatt
      961 gattctttaa aggaggtcgg agtgccgaga atgagtgtcc gcatgccaaa atccattgaa
     1021 gcgcgggcga tttctttggg gaaataatac atatcaaaat tcgcggtaat accgctggtc
     1081 aaatattcgg caaaagctaa tttggacaga tgatagacat cgctcggctg aagttttgcc
     1141 tcgatcggaa aaacccagtt gtaaagccag tcgcgaagcg ctaaatcatc ggcgtaactt
     1201 cttaaaaaag tcatcgccga gtgcgtatgg gcgtttttaa atccgggcat caaaacattt
     1261 ccgtcacata atatgacgcg atcaaaggga ccttgtcgcg aaccgttatg accgagataa
     1321 acaatgcggt ttccgtcgac gacgagatcg ccgcggaata ggggcttatc gttcatgctg
     1381 agaatgcggg catcttcaaa taatatgcgc atttcaaatc ctccttataa atatgctcaa
     1441 gaagcaaagc atatcattgt tttttcggtt ccggaagcgg acgaagtttt tctttgggag
     1501 cgccgcatac cgggcatgtt ccgtcttcgt ttggcacgac gatctgcccg catctaaggc
     1561 aacgatattt ttgcatagga aaatctcctt ataagataag tatagtatac gacatttgcg
     1621 gtgattttct aagcattata ctgcggcagt tatgaaagag cctttgtgtc gactttacct
     1681 actaaagtct tgggaagatg atcgcgatag acaatttcct tgggtaccgc ataaactccg
     1741 cagcgcttca aaactaccgc tttgatctca tcgtcgaaat ttgaggcctt ggccgcatga
     1801 tccagaacaa cgaacaattt aatggcattc ccgagctttt cgtcagcgat ggccaccgcc
     1861 gcgcattcgt gaaccgagcc catatccata accgcgcctt caatctcgtt aggaaagaca
     1921 ttgatgccgc ttaccttaat aatgcgtttt aacctttgcc gaaagtagac aaaaccatct
     1981 tcatccagac ttccgtaatc gccggtcgca atccagactt gtccatcttt gtcccgaaca
     2041 aacggttgat ccttttcgtc ttgcaaaaag cgatagccat tcatcattgt ttcgccgcgg
     2101 acatagagct cgccgtcacg accgaaaggc atgtcgcttt tagtgtcgag atcgacaact
     2161 ttcatgtcga cattgggaag aatttttccg actgttccgg ctttgtgaaa acgatggtta
     2221 ttaacggagc aaaccgtgac gacttcagtc aggccatagc cttcataaag ccgggcggat
     2281 gaaccatatt tttccatcag attattgaag cgttcaaaaa gggacggggc gacaaagtct
     2341 cccccgacaa aacagatgag cagattttta agggccggac cgcgaaagtg cttattccgc
     2401 aagagcgctt caaataaaat cgggacgccg atgataaagg aaatctgttt cttttttaaa
     2461 tattttacca cttgttctga gtgaaattta ggcatgagtg tattgcaggc gccaaacgct
     2521 agacaggcat gcattcccat gcacaaaccg aagccgtgaa agatgggaag gacggcgagc
     2581 atgaacttgt ctttggtgtc gttaattcct aaaatgtcaa gcccgttact ggccagcgca
     2641 ttgatgctaa aagaggaaag ggccaccact tttggtttgc cggtcgtccc cccgctgtgg
     2701 aggtaaatag cgtctttcgt aaaatcatta tcgcatgcaa aaacgccttc tttttgataa
     2761 aaagacagga ttttgtcgct atttgcaata tttttgattt tacttcggtt ctgcaacgaa
     2821 tagatcgttt ttacgagcgg atttaaagtt tggactgggg agcaagccac gacgcgaata
     2881 ccgagtttag tcaaatcggt gaactccgca aaaaaactgt ccaagacgaa caggagcttg
     2941 cttttcgtct cctccatgat gcctttcatc tgctcgtatt tcatcagagg atggatgaga
     3001 ttggcgatgg ccccgagctg gttgatggca tataaaagat ggacggcact cggaatatta
     3061 gggaggcaga cggagacgac atcatcggtt ttgaccccgt agtcggccag acctttcgct
     3121 agacgattaa catcgcgcaa aagcccttca taagtaattt tttgccgcat atatatgagt
     3181 gcgtttcgct tcggatagcg tgtgctggtg gcgagcaagg caccatataa agtcgtgttt
     3241 tttttcatgt gattcctctt attttaaagg tatgccaagc aggatgaaga tcaaggcggt
     3301 cagggccgct aaaacaacct ggtgtaacag gtagatatat atcgtctttg aaccgatgaa
     3361 cgtaagaggt cgaaagactt tttcaacggg gtgccaaaca acgttttctc ttttgaaaat
     3421 caggctcttt ttctgggcgt ataaaagctc gcccaagaaa ccgccaaaaa aggtatatcc
     3481 aagataagga aagaggccgg cccagtcgac gccataccac aggcgtccca aaacgatatc
     3541 aagcttgacg tccagaccat agacaatcgg cgtgaatacg ccctgaataa tccgatacga
     3601 attaatcgtg ggcgtcattc tgccgttaat aataaaccac caattttggg catcgacggg
     3661 cacggtggcc acttcaaggc gcatccaata accgacgccg gtaaataaga tggccagaaa
     3721 aagtgaggtc caaagccaaa aagtctttga ctttgtcatc ttgataataa gcgtccggaa
     3781 aagcgaatag gtgatcatcg ctaagccaag gcataagatg atgccaaaga agatgaacaa
     3841 atcggttttt aatgtgattg acaaaaagta ggaaaaaatg gaaatgatgg ctccaaagcc
     3901 gaggacgacc atcccgcgct tgatgctgtt gcgcgataaa taggtgctca gaccgcttaa
     3961 aataaaaaag agggcgataa ccgcataacg gaaattaatg cgccacgcct cgtaccaata
     4021 attatagcac cactcgatta gatcgcgcag agcctgaggg gcgtcagaaa caagaaaaaa
     4081 agaaggcgcc agctggccaa aatcataggc gagatgatcg accaccatca tgatgattaa
     4141 aagaccgcgc aggaggtcga tttcaaagat tcgcttcgcc ttctttttca tcgtgcgaag
     4201 tggaagatga agaaggtatc cgtctatcag ggccgtttcc atatagacaa tatagcaaaa
     4261 agaccctttt gtgtcttaat aaatcgcatg cttaatcgct tttttgttat tcgttgtgcc
     4321 cagatatata ttcggaatat gctttgtcaa tgttagcgaa gtagcggata aagcagcggt
     4381 aaaaaccctt ggactgccga gtgatatagg gaagggggag cgcttcattc ttttcgattt
     4441 cgaaaaagcg atatgcgccg ttgacgcgcc acgcgttgat atagccgatg cttatcgcgt
     4501 caaccgggca gaagaatgag cagcgcatgc acatctgaca ggaatgacga aatttgatct
     4561 taccatcttc ataatagata ttatgagtcg gacaatcgcg aacgcatttc atacacttaa
     4621 tgcacttgtt catgtcagtt cgataaagat aggaattaat cgttgccccc ggccgctgaa
     4681 tgccgatgat ccaagccagg aaattgtata gtatattagt tttcaaaaaa ctctttttcc
     4741 cttttattac attttcgaca agaatcttgc tgagcttgcg gttataaatc atgatttctt
     4801 ttacaaatgc atcatcaaag cgaaagtgaa tattatatgg catcacaaaa tgataatcgc
     4861 caagcacatc gcatttttct tttctaagct tgcggatgat tcttcggctc gaagcgttgt
     4921 tgagggcgag tgtttcaccg gaagatttga agataaaata ctttacattt ttgcatagtt
     4981 gcaaccgttt gacataacgc caaaaaaagc gtggctcatt gaaggcgtaa atcggatagc
     5041 ccaggcccac taaatcataa ttagatatat cgattaaagg cgtgtcgatg ttgatattaa
     5101 cgatctcgga aacgtgtcct tttaaagcaa aatcgctcac caaaagacga gcaatttcta
     5161 aagtgttatt cgttcccgaa aaaaggagaa ataaaattct catgctgaag ggtatttaat
     5221 cttctgttat ttccggcatc gcctcgagat c
//
LOCUS       DAOSSL010000046         5002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_15268_length_5002_cov_19.103497, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000046 DAOSSL010000000
VERSION     DAOSSL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15268_length_5002_cov_19.103497"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /gene="uvrA"
                     /locus_tag="PLH17_04350"
     CDS             <1..2097
                     /gene="uvrA"
                     /locus_tag="PLH17_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOR21032.1"
                     /translation="HHSCKYCGFSVPKLEPRLFSFNAPLGYCPKCKGLGINREAAFDL
                     LVPNDNLSIMQGAIRYYKNVVYSKNIDWQEFKYLCEQYKIDLNIPFKNLSDYQKKIVF
                     YGSDKPFTYVITSVSGNKNHRSGYIEGIKTKIDRLYLETTSSMMRDYYEIYMRDSECT
                     ECHGARLNNQVLAVRINQLNIYEVTTLPIKKLLKYIAELDEVLTPNEKEIGQLVIKEI
                     RNRTQFLSDVGLDYLSLSRMSMTLSGGEAQRIRLATQIGSRLSGVLYVLDEPSIGLHQ
                     KDNAKLLATLKSMRDLGNSLIVVEHDEETIRSADHIVDIGPGAGIHGGYIVAQGNLKA
                     IIDNKKSITGDYLSGRKKIEVPSARRKGNGRFVTVKGARCNNLKNITVDFPLGKFIAV
                     TGVSGSGKSSLVSQTLNRAIEKRLNGIDNFPGEHDDIFGLENIDKVVNVSQEPIGRTP
                     RSNPATYTKVFDDIRALFAETIEARARGFDKGRFSFNVIGGRCENCQGAGVTRIPMNF
                     LPDVYVRCDSCNGKRYNEETLQVTYKGKNIFEVLEMTVEDSLSFFENRPKIRRKLQTL
                     FDVGLGYIKLGQQATTLSGGEAQRVKLAFELQRRPTGKTLYILDEPTTGLHTDDVKRL
                     IAVLQKIVDNGDTVLVIEHNLDVIKVADHIIDIGKDGGDAGGQIIAQGTPEEIAACPD
                     SYTGHYLANILKGKNN"
     gene            2146..3438
                     /gene="lgt"
                     /locus_tag="PLH17_04355"
     CDS             2146..3438
                     /gene="lgt"
                     /locus_tag="PLH17_04355"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOR21033.1"
                     /translation="MEALGYISLIAVIAAFIFLHLTLKKIVKNESATSLSRQDLIKIF
                     SAIGGAALFAFLAMLGLILAKGWNLRGGEYAMALIGSFVFILGLSSLYAAFGLNFYKP
                     TLGANILKIVRLVMYIAIPVIIIFLALATEGIADYLTYPLVNRISLTEGFVDPFNRTA
                     QFAVAFYGILIVSGAVIVYFVSDHFFFKKFKRHGILDSTFYVAFPAGLVGARLWYCYV
                     LEFDRYANDFLAVLQVWDGGLAIMGGAILGIIAGVTFMLVRRKYVNIRWAMDVIVPAI
                     LIAQAIGRWGNFFNIEVHGLEVAASSWSFLPSIISNNMAFSSTDGPAAAGNIYVPLFL
                     IESISNLAGYFLITYGVGKGLRKWLSLGDLSMAYLIWYGATRAIMEPLRSADFEYGQS
                     WISSFILIGVGVLGIVAFHVYDFVRKKKNLEPRTYETV"
     gene            3445..4095
                     /locus_tag="PLH17_04360"
     CDS             3445..4095
                     /locus_tag="PLH17_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOR21034.1"
                     /translation="MYKLVLFDMDGTIADTDGMIVATFIEMYRLYRPDYMPTIDYMLT
                     FSGPPIVQTLTKEFPDLTFDYAFKEYQRISLPNYQKFAKAFPFVRELAEKLHARGIKI
                     GIVTSKHRYPAEYTLKLIDLDGVFDLLIAHDDVKEAKPNPEGIFQAMKHFGISDKRDV
                     LYVGDTLTDYLTAQNAGIDVALVTWTPRVLPPEAKPTYYIDSFNEFFEVINHGKTN"
     gene            4079..5002
                     /locus_tag="PLH17_04365"
     CDS             4079..5002
                     /locus_tag="PLH17_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOR21035.1"
                     /translation="MEKQIDVAIIGGGIAGITAGLYVKRSNLSVMAIDRNVFGGKLAK
                     LGEIYNYPGFEAISGPELAYKLMEQATKNDIELEYGSVINVTKQDDGLFLVETEEHMI
                     RAKVVIIATGTIEKQLGIPGEKEFLNRGVSYCATCDGPLYRNKEVAVVGYLDTALEEA
                     IYLSGIAQTVHLISKASVKEIESDKLSHVLSRSNINLIAKSDVMTIKGHDHVESIIVM
                     DLDTNDEQEIKVSAVFPYIGQKTLTEFLANLKVETRNGYLVVNKDMETNVPGLLAAGD
                     TTDKKLRQLVTAAGDGCIAATSAIRYIKAKK"
BASE COUNT         1324 a         1223 c         1147 g         1308 t
ORIGIN      
        1 catcacagct gcaaatactg cggtttttcg gtgccgaaac tcgaaccgcg cctcttttca
       61 tttaacgctc ctcttggcta ctgtcctaaa tgcaaaggac ttggcattaa tcgcgaggcg
      121 gcttttgatt tgctcgtgcc gaatgataat ttatcaatca tgcaaggcgc gattcgctat
      181 tacaaaaatg tcgtctacag taaaaatatc gattggcagg agtttaaata cttatgcgaa
      241 caatataaaa tcgatttaaa catacccttt aaaaacttaa gcgattatca aaagaaaatc
      301 gtcttttacg gaagcgataa gccttttact tatgtgatta catccgtcag cggcaacaaa
      361 aatcatcgct ctggctatat cgagggcatc aaaacaaaaa tagatcgcct ctacctcgag
      421 acgacaagct cgatgatgcg cgactattac gaaatatata tgcgcgacag cgaatgcacc
      481 gagtgccacg gagcgcggtt gaataatcag gttctcgccg ttcgcatcaa tcaactcaat
      541 atttatgaag tgacgacctt gccgattaaa aaattgctta aatacatagc tgagctcgat
      601 gaggtgttga cgcccaacga gaaagaaatc ggtcagctcg tcattaaaga aattcgcaat
      661 cgcacgcagt ttttaagcga tgtcggtctc gattatttat ctctttcgcg catgtcgatg
      721 acgctcagcg gcggtgaagc ccagagaatt cgtttggcca cgcagatcgg cagccgcctt
      781 tccggcgtct tatatgttct tgatgagccg agcatcggcc ttcatcaaaa agataacgcc
      841 aaattgctgg cgacgcttaa atcgatgcgc gatttaggca actccttaat cgtcgtcgaa
      901 cacgacgagg agacgattcg ctccgccgat catattgtcg atatcgggcc cggcgccggt
      961 attcacggcg gctacatagt cgcgcaagga aatcttaagg ccattatcga caacaaaaaa
     1021 tcgatcaccg gcgattatct ctcgggtcgc aaaaaaatcg aagttccctc cgcaagaaga
     1081 aaaggcaatg gccgctttgt gactgtcaaa ggagcgcgtt gcaacaactt aaaaaatatt
     1141 actgtcgatt ttcctttagg taagttcatt gccgtcactg gtgtctctgg cagcggaaaa
     1201 tcttcgctcg tctcgcagac gcttaatcgc gcgattgaaa aacgccttaa cggcatcgat
     1261 aattttccgg gtgagcacga tgatattttt ggtttagaaa atattgataa ggtcgtcaat
     1321 gtttcgcaag agcccatcgg ccgcacaccc aggagcaatc cggcgaccta tacgaaagta
     1381 tttgatgaca ttcgcgcctt gtttgccgag acgattgaag cgcgggcccg gggatttgat
     1441 aaaggtcgct tctcttttaa tgtcatcggc ggacgttgcg aaaattgcca aggagccggc
     1501 gtcacgcgga ttccgatgaa ctttttgccc gatgtctatg tgcgctgcga ttcgtgcaat
     1561 ggcaagcgtt ataacgaaga gactttacaa gtaacctata aaggaaaaaa tatctttgaa
     1621 gtgctcgaaa tgaccgtcga agattcattg tcattttttg aaaaccgccc caaaatacgg
     1681 cgaaaactcc agaccctatt tgatgtcggc ttaggttaca tcaaacttgg ccagcaggct
     1741 acgacattga gcggcggtga agcccagcgc gtcaaattgg ctttcgaatt gcagcgtcgc
     1801 ccgaccggca aaaccttata tattctcgac gaaccgacga ccggtttgca caccgatgat
     1861 gtcaaacggc tcattgccgt tttgcagaaa atcgtcgata acggcgacac cgtgctggta
     1921 atcgaacata atctcgatgt gattaaagtg gccgatcata tcatcgacat cggcaaagat
     1981 ggcggcgatg ctggcggtca gattatcgct cagggcacgc ctgaagaaat tgccgcctgc
     2041 cccgattcct acaccggcca ctatttggcg aacattctga aagggaaaaa caattgagtt
     2101 gttgaacaac aacaataatg ttattctttc aaacaggtaa tcaacatgga agcactcgga
     2161 tatatcagcc tcatcgccgt cattgccgct tttatctttt tacatctaac gttaaagaaa
     2221 attgtcaaaa atgaaagcgc gacatcatta tcgcgtcagg atttaatcaa gattttctcc
     2281 gcgatcggcg gcgccgcgct ttttgccttt ttggcgatgc tgggactcat ccttgcgaag
     2341 ggttggaatc ttcgcggagg cgaatacgcg atggccctta ttggaagttt tgtcttcatc
     2401 cttggattat catcgctata tgcggctttt ggtctaaatt tttacaaacc gacattagga
     2461 gccaacattc ttaaaatagt tcgcctcgtc atgtatattg ccatcccggt catcatcatc
     2521 tttttggcgc tggccaccga aggaattgcc gactatctga cttatccgct cgtcaaccgc
     2581 atctcgctca ccgaaggatt tgttgatccc tttaatcgca cggcccagtt tgccgtcgct
     2641 ttttatggca ttctaatcgt cagcggcgcc gtcatcgtct acttcgtcag cgatcacttc
     2701 ttttttaaaa aatttaaacg ccacggcatt cttgactcga cattttatgt cgcctttccg
     2761 gccgggttag tcggagcccg tctgtggtac tgctatgttt tggaattcga ccgatacgcc
     2821 aatgatttcc tcgccgtctt gcaagtgtgg gatggtggct tggcaattat gggcggcgcg
     2881 attcttggta ttatcgccgg cgtcactttt atgctcgtgc gccgcaaata cgtcaacatt
     2941 cgctgggcga tggacgtcat cgtcccggct attttaatcg cccaggccat cggccgctgg
     3001 ggcaatttct tcaatattga agtgcatggt ttagaagtcg cggcttcgtc gtggagcttc
     3061 ttaccgtcga ttatttccaa taacatggcc tttagttcaa cagacggacc ggccgctgcc
     3121 ggaaatatat atgtgccgct attcttgatt gaatcgatca gtaatctcgc cggctacttc
     3181 ctgattacct atggggtggg caaggggcta cgcaaatggc tctcgctcgg cgatttatcg
     3241 atggcttatc tcatctggta tggcgcgaca agagcaatta tggagccgct ccgctcggca
     3301 gactttgaat atgggcagtc gtggatttcc tcgttcattt taatcggtgt cggcgtcctc
     3361 ggcatcgtcg ctttccacgt ctacgacttt gttcgcaaga aaaagaatct tgaaccgcgg
     3421 acttacgaaa cggtgtaatt aatcatgtat aaactcgtac tttttgatat ggacggcacc
     3481 atcgccgata ccgatgggat gatcgtggcg acttttattg aaatgtatcg tctttatcgc
     3541 cccgattata tgccgactat tgactatatg ctgacctttt ccggtccgcc gattgtccaa
     3601 acattgacta aagaatttcc tgatttgact tttgattatg cttttaaaga gtatcagcga
     3661 atatcattgc caaattatca aaaatttgcc aaagcctttc cttttgtgcg tgaattggcc
     3721 gaaaaactcc acgctcgcgg aattaaaatc ggcatcgtta catccaagca tcgctatccc
     3781 gctgaataca ctttaaaact tatcgacctc gatggtgtgt ttgacctttt aatcgctcat
     3841 gacgatgtaa aagaagccaa accgaatcca gagggaattt ttcaagcgat gaaacatttt
     3901 ggcatcagcg ataagcgcga tgtcttatat gtcggcgaca cgctcaccga ttatctgacc
     3961 gcgcaaaatg ccggaatcga tgtcgccttg gtcacatgga ccccccgcgt tctccctcct
     4021 gaagcaaaac caacctatta tatcgactct tttaatgaat tctttgaggt gattaaccat
     4081 ggaaaaacaa attgatgtcg ccatcatcgg cggcggaatc gccggcatca cagccggtct
     4141 ttatgtcaag cgcagcaatt taagcgtcat ggctatcgac cgcaatgtct tcggcgggaa
     4201 actggccaaa ctcggtgaaa tctacaacta tccgggcttt gaggccatca gcggacctga
     4261 actcgcctat aaacttatgg aacaggccac taaaaacgat atcgaactcg aatacggaag
     4321 cgtcatcaat gtcacaaaac aagatgacgg actcttttta gtggagaccg aagagcacat
     4381 gattcgcgcg aaagtcgtca tcatcgcgac cggaacgatt gaaaagcagc tgggcattcc
     4441 cggcgagaag gaatttctta atcgcggcgt ctcgtactgc gcgacttgcg acggccccct
     4501 ctatcgcaat aaagaagtcg cggtcgtcgg ctatcttgat acggctcttg aagaagcgat
     4561 atatctctcc ggaatcgctc aaaccgttca tttgattagc aaagccagcg tcaaggaaat
     4621 cgaatcggat aaactctcgc acgttctgag ccgcagcaac atcaatctca tcgctaaaag
     4681 cgatgttatg acaataaaag gtcacgatca cgtcgaaagc atcatcgtca tggacctaga
     4741 caccaatgac gaacaagaaa ttaaagtcag tgctgtcttt ccttatatcg ggcaaaaaac
     4801 actgacggaa tttctcgcca atctgaaagt cgagacgcgc aacggctatc tcgtcgtcaa
     4861 taaggacatg gaaacgaatg ttcccggctt attggcggcc ggagatacga ccgataagaa
     4921 acttcggcag ctggtgacag cggccggcga tggctgcatt gcggccacca gcgccatccg
     4981 ctacatcaaa gccaaaaaat aa
//
LOCUS       DAOSSL010000047         4751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_16312_length_4751_cov_14.952939, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000047 DAOSSL010000000
VERSION     DAOSSL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16312_length_4751_cov_14.952939"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..666)
                     /locus_tag="PLH17_04370"
     CDS             complement(1..666)
                     /locus_tag="PLH17_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOR21036.1"
                     /translation="MANELDEMTGPVNQEGRDVNVIEKQIPVKVGTGSLVFEIILWVL
                     GIIPGLVFLFMKISARNYLRQLQQKIQHNASQIDNYLEQRVQILQNVVGIVEKSVDLD
                     KDVMKTVAAFRGGANPNEGNRNEVAGQLDTAMSTIRVAFEAYPDLKAHRALADAMQQN
                     SYLQREITAAREVYNDTVLKWNQDIFAWPTKMIVAAKAGYSTRIPFTASKETKDQARA
                     KFF"
     gene            complement(713..>1540)
                     /locus_tag="PLH17_04375"
     CDS             complement(713..>1540)
                     /locus_tag="PLH17_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21037.1"
                     /translation="VKKGGDYTVMGNSEFEVLFGASNRNNEVQFRLLFTPLAQKQLLQ
                     LMKDKEVAYGDDFDFVKHKMINIIYPEHLHGVNLDPNPSFFRGYDYDAVRKLFIDYHN
                     NYFKQVFFTFAPILAIPLYQQHKPHEYIYKDLYDSYVSFYEHEHVVNAMNESEFAHKL
                     SGTRNILKTSVVKSGGFCDTIRVTAYGYQTINRTDYIPVMGGDGRSHNVPVDWVEYIP
                     VSQDTDVNVNAVGEEKEETYADKFKKAIESMRSGKLADDKDVYTVGLFLAYIIKKSR"
     assembly_gap    1541..1640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1641..2404)
                     /locus_tag="PLH17_04380"
     CDS             complement(<1641..2404)
                     /locus_tag="PLH17_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21038.1"
                     /translation="MENTSEFAGFDGENIFEPLLAYKNVYKDLYNKNTTEYFDGLVEK
                     SQIDIEANRETNRKIKKHEVERTEIVKKINKQSALRGFLIFLSVAGLVAAIAGIVMLV
                     QGVQSLLAPILMIVFGFALPILFIVLIVVIINKKLKELRGIKSKIDKIIEDLMKEAWT
                     QMTPLNDLFTPGMGVYLFRKTVPIINLDKMFDSKRLDYLVNKFGLSEAHDVNRSTLYV
                     QSGDIIGNPFFLANDLVHHLGTKTYYGELVIHYTTTS"
     gene            complement(2532..3677)
                     /locus_tag="PLH17_04385"
     CDS             complement(2532..3677)
                     /locus_tag="PLH17_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOR21039.1"
                     /translation="MKRPNPFLWIPLAGLLKISAYFQGNRLVNKIKIKKPAIILSNHA
                     SFRDYIYVTAAVYPHRINYMAAAKMFYEPERRPFLKLARAIPICSYQGDLKAVASALQ
                     ILKKGGIIGIFPEGQITYHGKSLKSPFAIAKFLKKAKVNVYVVHDKNAFLMAPPWTNK
                     VFKGKVFTSMAHLFTAEQLEQMDEKSIYDAVSQSLEFDAGAYNQEHRYIYRLNDIANL
                     ENLIYQCPDCGHEGLVSQAHKLVCPKCHRELVYDQYGLLDGMSLFQNYERQRLKLEKL
                     ISDTPDYSLETPVRLVRFQGNGLGETGRGTISLNRELYTYRGTDKGEQVEYHFPTKLI
                     QYLPVDIGGDIQIYDNYEVFQFYMDTKYLPIKFSTAGEYFYRLAHNQ"
     gene            complement(3682..>4751)
                     /locus_tag="PLH17_04390"
     CDS             complement(3682..>4751)
                     /locus_tag="PLH17_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOR21040.1"
                     /translation="SYLIFVAVQSSVMIPYYSLSSEISKDYQTRASFNSYRLGFSIFS
                     SIVCVAIPGLIISSVPDARLGYQIMSLSFGVFFGISVLLSGLFAKEEIISPVVKTKLT
                     FKSLFEPLSLKPFRQYLLMLLLMQIAMAIMSGLFFFYINYYVTQDIMAAGGPNHVGLV
                     AAALMFSTQIVALPFYLKMIKIKSKAFTYRLGAVIWIISALGLLFLRPNVNEFVIYTF
                     AIVMGFGISAPGLIPHAMFGDVADAGQIKFSKRVEGQMSGFANLVNQISQGLGLGLAM
                     FILGFAGFKESPAGGEPILIQPDSAVLAIQLIMSLTPLIFLGIGSLVSLFYRIDAKEQ
                     QKIKEQIATINTEPVSVESLS"
BASE COUNT         1274 a          963 c         1113 g         1301 t
ORIGIN      
        1 ttagaaaaat ttagcgcggg cttgatcttt tgtttcttta ctagcagtaa agggaatgcg
       61 agttgaataa ccggctttag cggcgacgat catcttagtt ggccaagcaa agatgtcttg
      121 gttccatttt aagacggtgt cgttataaac ttcgcgggcc gccgtaatct ctctttgcaa
      181 gtagctgttt tgctgcattg catcggctaa agcacgatgg gctttaagat ccggataggc
      241 ttcaaaggca acgcggatgg tgctcatcgc ggtgtcaagt tgaccggcga cttcattgcg
      301 gttaccttcg ttggggttgg cgccaccgcg gaaagcggcg acggtcttca tcacatcttt
      361 atcgagatcg acggattttt cgacgatgcc gacgacgttt tgcagaattt gaaccctctg
      421 ttcgagataa ttatcaatct gcgaagcatt gtgctgaatc ttctgctgca gttgccgtag
      481 ataattgcgg gcgctgattt tcatgaagag gaaaacaagg ccgggaataa tgcctaaaac
      541 ccagagaatg atttcaaaca ccaatgagcc cgttcccact ttcacgggaa tctgcttttc
      601 gattacgtta acgtctcttc cttcttgatt gactggtcca gtcatttcgt ctagttcatt
      661 tgccattttt agatacctcc atctttatat tatatatata cttggctaag tgttatctac
      721 tttttttaat aatatacgcc aggaataatc cgacggtata gacatcttta tcatcggcga
      781 gcttgcctga gcgcatcgac tcaatcgctt ttttgaactt gtcggcatac gtttcttctt
      841 tttcttcgcc gacggcattg acattgacat ccgtatcctg cgagacaggg atgtattcta
      901 cccaatcgac tggcacgttg tgcgagcggc cgtcaccgcc catcaccgga atataatcgg
      961 tgcggttaat cgtctgatat ccgtaggccg tgacgcggat cgtatcgcag aagccgccgc
     1021 ttttgacgac cgaagtcttt aagatattgc gcgtccccga tagtttatgg gcaaattcgg
     1081 actcgttcat ggcgttgacg acatgttcgt gctcatagaa gctcacatag ctgtcataga
     1141 gatctttgta gatgtattca tggggtttgt gctgctgata gagagggatg gcgaggattg
     1201 gggcaaaagt gaagaatacc tgcttaaaat aattgttgtg atagtcgatg aataacttcc
     1261 ggacagcatc gtaatcatag ccgcgaaaga aactcggatt aggatcgaga ttgacgccgt
     1321 gcagatgttc gggataaata atgttaatca tcttatgttt gacaaagtca aagtcatcgc
     1381 catatgccac ttctttgtcc ttcattagct gcaggagttg cttttgggct agaggcgtaa
     1441 aaaggaggcg aaactgaact tcattgttgc ggttagaagc gccgaacaag acttcgaatt
     1501 ccgagttgcc catgacggtg taatcgccgc ctttcttgac nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn gatgtcgtcg tgtaatgaat gacgagttcg ccataatatg
     1681 tttttgtccc gagatgatgg acgaggtcgt tggcgagaaa aaagggattg ccgatgatat
     1741 cgccggattg gacgtaaagc gtcgagcgat tgacatcatg ggcttcggag agaccgaatt
     1801 tattgacgag ataatcgagg cgtttgctgt caaacatctt gtcgagatta ataatcggta
     1861 ctgttttacg aaaaagataa acgcccatac ccggcgtgaa taaatcgttt agaggggtca
     1921 tctgcgtcca agcttctttc attaaatcct cgatgatttt gtcgattttg gacttaatac
     1981 cgcgcaattc cttaagtttt ttattaataa tgacaacgat taaaacgata aaaaggattg
     2041 gaagagcaaa gccgaagaca atcattaaaa tcggggcgag taagctttgt accccttgaa
     2101 cgagcatgac gataccggcg atggcggcga ctagtccggc gacgctcagg aaaataagaa
     2161 agccgcgtaa agcgctttgc ttgttaatct ttttgacgat ttcggtgcgc tcgacttcgt
     2221 gctttttaat cttccggttc gtctcgcgat tggcttcgat gtcgatctgc gatttctcga
     2281 cgagaccatc aaaatattcg gtcgtatttt tgttatacaa atccttgtag acgtttttgt
     2341 aggcgaggag cggctcaaaa atgttttcgc cgtcgaaacc cgcaaattca cttgtgtttt
     2401 ccatagatta gtgcccctta tcaagatata aacaagccta aaggcgctcg ttctttttca
     2461 ttgtagcaca accaaatgaa gacgattatt tttttaagga gttaattggc atttgttttc
     2521 aatagaaaaa tctattgatt gtgggcgagg cggtaaaaat attcgccagc ggtcgaaaac
     2581 ttgatcggca gatatttggt gtccatataa aactgaaaca cttcgtaatt gtcataaatt
     2641 tgaatgtcgc caccgatgtc gacgggtaaa tattgaatga gctttgtcgg aaagtgatat
     2701 tcgacttgct cgcccttatc cgtgccgcga tatgtgtaga gctcacgatt gaggctgatg
     2761 gtgccgcgcc ccgtctctcc taagccatta ccttgaaagc ggacgagccg cactggagtc
     2821 tcgagagaat agtctggtgt atcggaaatc aatttctcca atttcaggcg ctgtctttca
     2881 tagttttgaa aaaggctcat cccgtcgagc agaccatatt ggtcataaac caattcgcga
     2941 tggcatttcg ggcagacgag tttgtgcgct tgactgacga gaccttcgtg accgcagtcg
     3001 gggcattgat agatgagatt ctcgagattg gcgatatcgt tcaggcggta gatatagcga
     3061 tgctcttgat tataagcgcc ggcatcgaat tcaaggcttt ggctaacggc atcatagatg
     3121 cttttttcgt ccatctgctc cagttgttcg gccgtgaaaa ggtgggccat ggaagtaaat
     3181 actttgcctt taaaaacttt gtttgtccat ggaggagcca ttagaaaggc gttcttatca
     3241 tggacgacat agacattaac cttcgctttt tttaaaaatt tggcgatagc gaaaggcgat
     3301 ttgagactct tgccgtgata agtgatttga ccttcgggaa aaatgccgat gataccgcct
     3361 ttttttagta tttgcaaagc gctggcaacg gcttttaaat cgccttgata tgagcagatg
     3421 ggaatggcgc gggccaattt taaaaaaggt cggcgttccg gttcataaaa catcttggcg
     3481 gcggccatat agttgatgcg atgaggataa acggcagccg tcacatagat atagtcgcga
     3541 aaagaagcgt gattggaaag aatgatggcc ggctttttaa tcttgatttt attgacgagc
     3601 cgattaccct gaaaatatgc cgaaatcttc agaaggccgg cgagaggaat ccacaaaaaa
     3661 ggattaggtc gtttcatatc tttatgaaag cgactccaca ctcactggtt cggtatttat
     3721 ggtagctatt tgctccttga ttttctgctg ttctttggcg tcgatacgat aaaaaaggga
     3781 gacgagagaa ccgatgccca gaaagattag aggagttaat gacataatga gttgaatggc
     3841 caatactgcg ctatcgggtt gaatcaaaat aggctctccc cccgccgggc tttctttgaa
     3901 gccggcaaac cctaaaatga acatagctaa accgaggccg agtccttgag aaatctgatt
     3961 gactaaattt gcgaacccgc tcatctgacc ttccacgcgt tttgaaaact ttatctgccc
     4021 ggcgtcggca acatcgccaa acatcgcatg aggaattaag ccgggggcgc tgatgccaaa
     4081 tcccatgacg atggcaaaag tatatatgac gaattcattg acgttcggtc gaagaaaaag
     4141 caaaccgagt gccgaaataa tccaaatcac agcgcccaag cgatatgtaa aagctttgct
     4201 tttaatcttt atcattttta aatagaaagg cagggcgaca atctgcgttg aaaacatcag
     4261 cgcagccgca actaagccaa catgattggg tccaccagca gccatgatgt cttgtgttac
     4321 gtaataattt atataaaaaa agaaaagtcc gctcatgata gccatagcga tttgcattag
     4381 aagcaacatt aaaaggtact gtcgaaaagg ctttaaagac aaaggttcaa acaatgactt
     4441 gaaagttagt tttgttttga cgaccggact tatgatttct tccttggcaa ataagccgct
     4501 tagcaaaacg cttattccga aaaagacgcc aaatgagagg ctcatgatct ggtaaccgag
     4561 tcttgcgtca ggaaccgaag aaataatcag tcccggaatc gccacgcaga caattgaaga
     4621 aaagatactg aagccaaggc ggtatgagtt aaaggatgct cgtgtttgat aatctttgga
     4681 tatttcgctt gatagcgaat aatacggaat cattaccgac gattgaaccg caacgaaaat
     4741 caaatatgaa a
//
LOCUS       DAOSSL010000048         4688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_16598_length_4688_cov_13.694367, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000048 DAOSSL010000000
VERSION     DAOSSL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16598_length_4688_cov_13.694367"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..90)
                     /locus_tag="PLH17_04395"
     tRNA            complement(1..90)
                     /locus_tag="PLH17_04395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ser,seq:gct)
     gene            complement(139..1572)
                     /gene="proS"
                     /locus_tag="PLH17_04400"
     CDS             complement(139..1572)
                     /gene="proS"
                     /locus_tag="PLH17_04400"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOR21041.1"
                     /translation="METNKNEAITSRDVDFAKWYTDVCRKAELMDYSSVKGFIIYRPY
                     GYAIWEQIQNYLNSRFKGIGVQNVYMPLVIPESLFNKEKEHVEGFAPETLIATIGGKS
                     KIDDPLIIRPTSEVLFCEHYAKIVSSHRDLPKLYNQWCSVVRWEKTTRPFLRGSEFLW
                     QEGHTIFATEEEARQNALDILDIYDDMGSELLAIPFVKGLKTDKERFAGALETYSIEA
                     LMHDGKALQSGTTHYFGTGFAKAFGITFQGKDNVLRNPHQASWGVSTRLLGSIIMVHG
                     DDNGLVLPPFVAPIQVVIIPIQQNKEGILDKANEIKERLEKAGYRVSLDDSDRTPGWK
                     YAEHEMKGVPLRLELGPRDLEKDQVVIARRVDGRKSFVKNEDIESEVARLLRETHEEM
                     YRKAQEFLKSHIVEVKSVQELKNVVDVGGYAKMMWCGERACEDKIKELTTATARCMPH
                     GDHEQFADECAVCGKKAEKIVYFAKAY"
     gene            complement(1585..3312)
                     /locus_tag="PLH17_04405"
     CDS             complement(1585..3312)
                     /locus_tag="PLH17_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOR21042.1"
                     /translation="MPNFNVENTPQLILFIVMASLIFVILIIFLSFFISAHGDKKYRR
                     EIKEQANTVRVYIIDVKNNVVRFFNRSNIREQRTISVTDFYNQFPNFEREKLIEWIAA
                     LIDGNKNAADYFEIDVFTSKNKKHYLSMLQVQEVDRGKELIHLESYLLRYLPPKVSEG
                     KVVTASSMEEIARVLLDNSPFRGVVVDFNFFYKRSSTQNKAGSFDRLIFTHIKEAISN
                     HFTQNRYYLEPSQHEIVVFDTRLSSHSSIMLFVHSLVNDINRFLSLNSLIEQIGISVG
                     IVEHKFFPNDAEKMLRQAMVVAAYGQEDNELITWYEQGMRGDDTIEQSNRTEVENIIR
                     NKKLKFLYRPILDAEKMKVLGYQSFVQPFDTFFDSIGELKEYATRTEDDKELFGTISR
                     NVISRFINEKNGDYLRLFFDVLWQEKPYILKTFGHINRIKETHIVLVFSETDINNQTD
                     DDEIMISELRTYKVKGYEIALKLADKDLLLNSTVYDMFDFFILDEKMTHDINTNSRRR
                     LQMRSLVEKLLKYNKPIIATDLQNWNAVELVIKSGIHYVASETISPSDEMILPISQKN
                     LNKIKAMAS"
     gene            3480..3570
                     /locus_tag="PLH17_04410"
     tRNA            3480..3570
                     /locus_tag="PLH17_04410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3514..3516,aa:Ser,seq:cga)
     gene            complement(3604..4626)
                     /locus_tag="PLH17_04415"
     CDS             complement(3604..4626)
                     /locus_tag="PLH17_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOR21043.1"
                     /translation="MAKKHTLAGRFHSFLLDHIWLKYVVEYAVALFMCALSAAVFAVG
                     LVSFLTPASAELSFVSGGVSGISQTISLAAQLMLGDAIEPYRDIIYSVFYIAINIPII
                     ILAFRKIGVRFAVLTMINVALVSLFASTFRLIPFLNDLGAFVDLHGGMLSRTLFAGIC
                     TGLSSAIAFKYELSAGGADVIAYYVSLKKSTPTGKYTMMINTAIILVYGLLMAISPSL
                     VSSENDAWIMAASGVLFSIVYLFTSMLVIDFINVRNKKVQIQIVTKKDNLHTMLLANV
                     PHGATIIKGTGAFSGEARFIINMVVSSYETKKVVKLIRQIDPESFINVSNLQQVYGRF
                     YIRPVK"
BASE COUNT         1252 a         1042 c         1115 g         1279 t
ORIGIN      
        1 tggcggagta agagggagtc gaaccctcgc accagttgcc cgatctactt ccttagcagg
       61 gaagcccctt caccacttgg gtattactcc acgaacgcca ttaaatatac cataatggcg
      121 tcaaaaactt caaacaattt aataggcttt agcgaagtag acgattttct cggctttttt
      181 gccgcagaca gcgcattcat cagcgaactg ctcatgatca ccgtgcggca tgcagcgcgc
      241 cgtcgccgtc gtcaattctt taatcttatc ttcacaggct cgttcgccac accacatcat
      301 cttggcgtaa ccgccaacat cgacgacatt cttaagctcc tgaacacttt taacctcaac
      361 aatatggctc ttaagaaatt cttgggcttt gcggtacatc tcttcatgcg tttctcttaa
      421 aagacgagca acctcgcttt caatgtcttc atttttgaca aaagatttgc gaccatccac
      481 gcgccgagca atgacgactt gatccttctc gagatcgcgc ggtccgagtt cgaggcgaag
      541 aggtacaccc ttcatctcgt gttcggcgta tttccagccc ggtgtccggt cgctgtcatc
      601 gaggctgacc cgatacccgg ctttttcaag gcgttctttt atttcgttgg ctttatctaa
      661 aattccttct ttgttttgct gaatcggaat gatgacgact tgaatgggcg ccacaaacgg
      721 aggtaacact aaaccgttat cgtcgccgtg caccataata atcgagccga gtaagcgcgt
      781 ggaaacgccc cagctggcct ggtgcggatt gcgcaagaca ttgtctttgc cctgaaaggt
      841 gatgccaaag gccttggcaa agcccgttcc gaaatagtga gtcgttccgc tttgaagggc
      901 ctttccatcg tgcattaaag cctcgattga atatgtttcg agagcgccgg cgaagcgttc
      961 cttgtcggtc tttaagcctt tgacgaaggg aatggcgaga agctcgcttc ccatatcatc
     1021 atagatatct aaaatatcaa gagcattctg gcgggcctcc tcttcggtag cgaagatggt
     1081 gtggccttcc tgccacaaaa attcgctgcc ccgtaaaaat ggccgcgtcg ttttttccca
     1141 gcggacgacg ctgcaccact ggttatagag cttaggtaaa tcgcgatgcg aagaaacgat
     1201 tttagcgtag tgctcgcaaa ataaaacctc gcttgtcggg cggataatca gggggtcatc
     1261 aattttactt tttccgccga tggtcgcgat taatgtctcg ggggcaaaac cttcgacgtg
     1321 ctctttttct ttgttaaaca aagattcggg gatgacgagg ggcatgtaga cgttttgcac
     1381 gccgatgcct ttaaatctac tatttagata attctgaatt tgctcccaga tagcgtaacc
     1441 gtaaggtcgg tagataataa aacccttaac cgaggaataa tccattaatt cggctttgcg
     1501 gcagacgtcg gtgtaccatt tggcgaaatc gacatcgcga ctcgtgatcg cttcgttttt
     1561 atttgtttcc atgctgagtt ctccttatga agccatggct ttaatcttat tgaggttttt
     1621 ttgcgaaatc ggcaagatca tctcgtcgct tggcgaaatc gtctcgctgg cgacatagtg
     1681 aatgcccgat ttgatgacca attccaccgc gttccagttt tggagatcgg tggcgattat
     1741 cggcttgttg tattttaaca gtttttcaac cagcgaacgc atctgcagac ggcggcggct
     1801 gttggtgtta atatcgtgtg tcatcttttc gtcaaggatg aaaaaatcaa acatatcata
     1861 gacggtcgaa tttaatagca gatctttatc cgcgagtttt aaggcgattt cgtaaccttt
     1921 tactttataa gtcctcaact cgctaatcat gatttcgtca tcatcagtct gattgttgat
     1981 gtcggtctcg gagaagacga ggacgatatg ggtttccttg atgcggttga tgtggccaaa
     2041 tgtttttaat atatagggtt tttcctgcca gagaacatca aagaagaggc ggagataatc
     2101 gccgtttttc tcgttgataa aacgggagat gacattgcgg gaaatagttc caaaaagttc
     2161 tttatcatcc tcggtgcgcg tggcgtattc ttttagttct ccgattgaat caaagaaggt
     2221 gtcaaagggc tggacgaacg attgatagcc taagactttc atcttctcgg catcgaggat
     2281 cgggcgatat agaaacttta gtttcttgtt gcggatgatg ttctcgactt cggtgcgatt
     2341 cgattgctcg atcgtgtcat cgccgcgcat cccctgctca taccaggtga tgagttcatt
     2401 atcctcttgg ccataggccg cgaccaccat cgcctgacgg agcatttttt cggcgtcgtt
     2461 tggaaagaat ttatgctcga cgatgccaac ggagatgccg atttgttcaa ttagactgtt
     2521 taagcttaag aagcgattaa tgtcattgac gagtgaatgg acaaagagca tgatgctcga
     2581 atgtgaggaa aggcgggtgt caaaaacgac aatctcgtgt tgtgaaggct caagataata
     2641 acgattttga gtaaagtgat tggagatggc ctctttaata tgtgtgaaaa tcaggcggtc
     2701 aaaagatccg gctttatttt gcgtcgaaga tcttttatag aagaaattaa aatcgacaac
     2761 gacgccccga aaaggcgaat tatccaataa cactcgcgcg atttcttcca ttgaactggc
     2821 ggtgaccacc ttgccttcgc tgaccttcgg cggcaaatag cgaaggagat aactctcaag
     2881 atgaattagt tcctttccgc gatcgacttc ctggacttga agcatcgata aatagtgctt
     2941 tttgttcttg ctggtaaaga cgtcgatttc aaagtaatca gcggcgtttt tgttgccgtc
     3001 gatcaaggcg gcgatccact cgatgagttt ttcgcgttcg aaattgggaa actggttata
     3061 aaaatcggtg acggaaatcg tcctttgctc gcggatgttc gagcggttaa aaaagcggac
     3121 gacattgttc ttgacatcga tgatataaac gcggaccgtg ttggcttgct ctttaatttc
     3181 ccggcggtat tttttatcgc cgtgagcgct tataaaaaaa ctcaaaaaga tgattaaaat
     3241 gacgaaaata aggctcgcca tgacgatgaa aagaattagc tgtggggtgt tttcaacatt
     3301 aaaattcggc acttgatgaa acctcgccta tcattataca cgaataagag cgcgatacaa
     3361 tgcgagttat ggaaaatagc ggtatccaat catagattgc tagggcttat tgccaccaac
     3421 taaaaagtcg cattctcctg tggcgacaag ttataaacat gctataatta tttaacgttg
     3481 gaggaatacc caagcggctg aaggggtcgg tttcgaaaac cgatagaaga tgcaagtctt
     3541 gcaagggttc aaatcccttt tcctccgcca gcaaaaaccg atgacggctc cgcccgtctt
     3601 tttttatttg accggacgga tgtaaaagcg gccgtaaacc tgctgcaaat tgctgacatt
     3661 gatgaagctt tcgggatcga tttgacgaat caacttgacg acttttttcg tctcataact
     3721 cgagacgacc atattgatga taaaccgcgc ttctcccgaa aaggcgccgg tacctttgat
     3781 aatcgtcgcg ccatggggga cattagcgag tagcatggta tgcaaattat cctttttagt
     3841 cacgatttga atctggactt ttttattgcg aacattgata aaatcaatga ccagcatcga
     3901 ggtgaaaaga tagacaatgg agaagagcac gcccgaggca gccatgatcc aggcatcgtt
     3961 ttcggagctg acaaggcttg gcgaaatagc cattaaaagg ccgtagacca ggatgatggc
     4021 ggtattgatc atcatcgtgt atttgccggt cggtgtcgat tttttaagag agacataata
     4081 ggcgatgaca tccgctcccc ccgccgataa ttcatattta aaggcaatcg cactactaag
     4141 ccccgtgcag atgccggcaa ataaagtgcg gctgagcatg ccgccgtgaa ggtcaacaaa
     4201 ggcgcctaaa tcattcagaa aaggtatgag gcgaaaagtg ctggcaaata gcgacacaag
     4261 cgcaacatta atcattgtta atacggcaaa tcgcacgcca attttgcgaa acgccagaat
     4321 aataatcggt atattaatcg cgatatagaa gacagaatag atgatgtcgc ggtatggttc
     4381 aatcgcatca cctagcatga gctgggcggc gagactgatc gtttgcgaga tgcccgatac
     4441 gcctccggaa acaaaggaaa gttctgctga tgccggagtc aagaagctga ccaatccgac
     4501 tgcgaaaaca gccgcggaaa gagcgcacat aaataatgcg acggcatatt cgaccacata
     4561 tttgagccag atatgatcga gcaagaagga atgaaagcga cccgccaaag tatgtttttt
     4621 tgccatcgtt agatttatcc tttgattatt gtagcaaaag cctataaaga tatacattta
     4681 tttcaaac
//
LOCUS       DAOSSL010000049         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_19469_length_4128_cov_15.384729, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000049 DAOSSL010000000
VERSION     DAOSSL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19469_length_4128_cov_15.384729"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..917
                     /locus_tag="PLH17_04420"
     CDS             57..917
                     /locus_tag="PLH17_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOR21044.1"
                     /translation="MPIKYQNVSFTYNAKTPFSYEALSDVSLDIIDGSFTAIVGKTGC
                     GKSTLIQQLNGLLVPTSGEVIINDYVVARSRRRRTKKIKELRKLVGLVFQFPEYQLFE
                     ETVEKDVAFGPKNFGASNEEALKSAHEALGLVGLDESFYKRSPFDLSGGERRRVAIAG
                     ILALNPNILVLDEPTAGLDPRGAREMMALFKGLHDNGTTVILVTHDMNIVLEYSDTVI
                     VMEDGEVREITTPLELFNKENDRYSLETPLLYKFVKSLSDKRMTLDIKMIKDIDSLAA
                     AIALALRNKR"
     gene            914..1786
                     /locus_tag="PLH17_04425"
     CDS             914..1786
                     /locus_tag="PLH17_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HOR21045.1"
                     /translation="MKSLSLGRYAPFNSFVHRLDPRSKLLILVLMMVAVFYGYSSQVM
                     TFVMGGILLLLIFVILLVSRVSLRQLFSSLKALWIMIIILLLINVLVPPLGASRIAFS
                     VGTYHIFWESILQSAKIILRLVMMISLTLILTATTKPLDLTYALEWYMYPLKLVKFPT
                     HEIAMTISIALRFIPTLLDETMRIMNAQASRGVDFEHGKVKEKIKAILSLIVPLFMSA
                     FQRSEELANAMEARGYNPRAKRTRYRNLHWGGGDTFSLLLATILTAGVILTSVMNWNY
                     VEMIFNITTWSVGL"
     gene            1783..2514
                     /gene="truA"
                     /locus_tag="PLH17_04430"
     CDS             1783..2514
                     /gene="truA"
                     /locus_tag="PLH17_04430"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOR21046.1"
                     /translation="MRYLATVKYNGTKYLGWQRQPGVATIQGSIETVISKLLDKETAI
                     YGSGRTDAGVHALGQTFHFDTEKEIADLEKFRYAMNRLLNRDIHIDSIKVVAEDFHAR
                     LSAKYKVYQYIINTGEDDPFNVDFKHFYPRNLDIEEMTKAARFFYGKHDFRNYTSKEE
                     DEDHFVREITGIHLYTHEEQIVIEITATGFMRYMVRMIVGTLIEIGMGRFTTAEAAML
                     IDNPNRSYVNYKAPPNGLYLVRVEY"
     gene            2547..>2890
                     /locus_tag="PLH17_04435"
     CDS             2547..>2890
                     /locus_tag="PLH17_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020862670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR21047.1"
                     /translation="MGRHFEVRAAAMAKSGARKSALYARASREIYMAAKSGLPDPKNN
                     LALRSAIDKFKGQSIPRDVIDRAIKKAAGGDVETYTAGRYEAFGPGNSFLMIDSLTDN
                     ANRAFVEIRTAVT"
     assembly_gap    2891..2990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2991..3126
                     /locus_tag="PLH17_04440"
     CDS             <2991..3126
                     /locus_tag="PLH17_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOR21048.1"
                     /translation="VAEIQMLANETITLGDEDKALFQELVDALDELQDVQNVFHNVDL
                     "
     gene            3259..3699
                     /gene="rplM"
                     /locus_tag="PLH17_04445"
     CDS             3259..3699
                     /gene="rplM"
                     /locus_tag="PLH17_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOR21049.1"
                     /translation="MQRQTTIAKAQEISRKWYVIDATDKPLGRLASEVAVVLIGKHKP
                     TFTPNVDCGDFVIIVNAEKVGLSGDKEHKKNYYNVSMQPGGLRTRSSGEMLKNYPEEM
                     LERAVWGMLPKGRLGRQIYKKLFVYKGPEHKHEAQKPETLELKY"
     gene            3711..4112
                     /gene="rpsI"
                     /locus_tag="PLH17_04450"
     CDS             3711..4112
                     /gene="rpsI"
                     /locus_tag="PLH17_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOR21050.1"
                     /translation="MATSKKSAQYYGTGRRKSSIARVYLVPGKGKITVNGFDVNAYMP
                     YETLVMDLKQPLVLTNNADKFDVNATVKGGGFTGQSGAIRLGITRALLEAGADRTTLK
                     VAGFITRDARTKERKKYGLKGARRAPQFSKR"
BASE COUNT         1114 a          984 c          927 g         1003 t
ORIGIN      
        1 aagcagggaa ttgatgtcga taactttgca aatctcggcg aaatggcgga atttttatgc
       61 cgataaaata ccaaaacgta tcgtttacat ataacgccaa aaccccgttt agttacgagg
      121 ctctttccga tgtcagcctc gatatcatcg acggttcctt taccgctatc gtcggcaaaa
      181 ccggctgcgg caaatcgacg cttattcagc aacttaatgg cctcctcgtc ccgacttccg
      241 gcgaagtcat catcaatgat tatgtcgtcg ccagaagccg caggagaagg acaaaaaaga
      301 ttaaagaact gcgcaagctc gtcggcctcg tctttcagtt tccggaatat cagctctttg
      361 aagagacggt cgaaaaagat gtcgccttcg gaccgaaaaa ttttggcgcg agcaatgaag
      421 aagccctcaa aagcgcccat gaagccctgg gcctagtcgg cctcgacgag agtttttata
      481 agcgttcgcc ctttgattta tccggaggcg aaagaaggcg cgtggccatc gccggcattt
      541 tagctttaaa tccgaatatc ctcgtcctcg acgaaccgac cgccggcctt gaccctcgcg
      601 gagcgcgtga gatgatggcc ctctttaaag gccttcacga taacggcacg accgtcattc
      661 tcgtcaccca tgatatgaat atcgtcctgg agtatagcga taccgtcatc gtcatggaag
      721 atggcgaagt tcgcgaaata accacacctc tggaactttt caataaagaa aatgaccgtt
      781 actcgctgga gacgcctctc ctttataaat ttgtaaaatc cctttccgat aaaagaatga
      841 cgcttgatat caaaatgatt aaagatatcg actcgctcgc cgcggccatc gctctcgccc
      901 tgaggaacaa acgatgaaaa gtttatcatt gggtcgctat gcccctttta acagctttgt
      961 ccatcgcctc gatccgcgca gcaaactttt gattctggtc ttgatgatgg tcgccgtttt
     1021 ctatggctat tcaagccagg tcatgacttt tgtcatgggt ggtattctct tactcctaat
     1081 ttttgtcata ttgctcgttt cgcgcgtcag ccttcgccaa ctcttttcct cactcaaagc
     1141 cctctggata atgataatta ttttgctgtt aattaatgtt ctcgtaccgc ctctaggcgc
     1201 ctccagaatc gccttcagcg tcggcactta tcacatcttc tgggagtcga ttctgcaaag
     1261 cgcaaagata attctccgct tggtgatgat gatctcctta actcttattt tgacggcgac
     1321 gaccaaacca ctggatttga cttatgccct cgaatggtac atgtatcctc taaaactggt
     1381 taaatttccg acccacgaaa tcgcgatgac gatttctatc gccctgcgct ttattccgac
     1441 cctcctcgac gagacgatgc gcataatgaa tgcccaagct tcaagaggtg tcgatttcga
     1501 acacggcaaa gtcaaagaga agattaaggc gatcttgtcc ttgatcgtcc ctttatttat
     1561 gtcggccttt caaaggagtg aagaactcgc taatgcgatg gaagctcgtg gctacaatcc
     1621 gcgggccaag cggacgcgtt accgcaatct ccactggggt ggcggcgata ctttctcttt
     1681 gctattagcg acgattttga ccgcgggagt aatcctcacc tcggtcatga actggaatta
     1741 cgtcgagatg atttttaata tcacgacgtg gagtgtcggc ctatgagata tttagccaca
     1801 gtcaagtata acggaacaaa gtatttaggc tggcaaagac aacccggtgt agcgacgata
     1861 caaggatcaa tcgaaaccgt gatctctaaa ttattggata aggaaacggc tatctacggt
     1921 tccggacgaa ccgatgccgg cgttcacgcc ctcggtcaga cttttcattt tgataccgaa
     1981 aaagaaattg cggatcttga aaaatttcgc tatgcgatga accgactttt aaatcgcgat
     2041 atccacatcg atagcattaa ggttgtcgcc gaagattttc acgcccgttt atcggcgaaa
     2101 tacaaagtat accaatatat catcaacacc ggcgaagatg atcctttcaa tgtcgatttt
     2161 aaacattttt atcctcgaaa tttggatatt gaagagatga caaaagccgc gcggttcttt
     2221 tatggtaaac acgatttccg caactacact agtaaagaag aagatgaaga tcactttgtc
     2281 cgcgaaatca ccggtattca tctctataca catgaggagc aaattgtcat cgaaattacc
     2341 gcaacgggtt ttatgcgtta catggtccgc atgatcgtcg gcaccttaat cgagatcggc
     2401 atgggccgct tcaccaccgc ggaagccgca atgttaatcg acaatccgaa tcgcagttat
     2461 gtcaactata aggcccctcc taacggtttg tatttagtaa gagtcgaata ctaaaatgaa
     2521 agaaagaata aattaggaga ataattatgg gaagacattt tgaagtaaga gccgcggcga
     2581 tggccaaaag cggcgcgcgt aaatcagcct tgtacgccag agcctcacgc gaaatttata
     2641 tggcggcgaa atccggcttg ccggatccga aaaacaattt ggccctgcgc tcggccatcg
     2701 ataagtttaa aggtcagtcg attccccgcg atgtcatcga ccgggcgatt aaaaaagccg
     2761 ccggcggcga tgtcgaaaca tatacggcgg gacgctatga ggcttttggc ccgggcaaca
     2821 gctttttgat gattgatagt ttgacggata atgccaatcg ggccttcgtc gaaattcgca
     2881 cggcggtcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtcgccgag
     3001 attcagatgc tggccaatga aacgattact ttgggcgatg aagacaaagc cctttttcag
     3061 gaattggtcg atgctctcga tgaactgcaa gacgtccaaa atgtgtttca caacgtcgat
     3121 ttgtaatgat ggcgaaaggt tttaaacagc caggaaataa tcattgacat ttctatatat
     3181 aatatatagt atttaatacg gcgcaaattg cgaaaactac ggtcccggta agattatagt
     3241 ttgagtaagg agaatattat gcaacgtcaa acaacgatag ctaaagcgca ggaaatatcc
     3301 cgtaagtggt atgtcatcga tgcaactgac aaacccttag gtagacttgc ttcggaagtc
     3361 gcggtggtcc taattggcaa acacaaacca accttcaccc ccaatgtcga ctgcggtgat
     3421 tttgtcatca tcgtcaatgc cgaaaaagtc ggattatccg gcgataaaga acacaagaag
     3481 aattattaca acgtctcgat gcaacccggc ggactcagaa cgagatcctc tggtgaaatg
     3541 ctcaagaact atccggaaga aatgcttgaa agagcggtgt ggggcatgct ccccaaaggc
     3601 cgactcggcc gccagattta caagaaattg ttcgtctata aaggtccgga acacaaacac
     3661 gaagctcaga agcctgaaac gcttgagctc aagtactagg aggacagtca atggccacat
     3721 ctaagaaatc cgcccaatat tatggaaccg gacggcgcaa atcgtcgatt gcccgcgtct
     3781 atttagttcc cggcaaaggc aagattaccg tcaacggctt tgacgtcaac gcctacatgc
     3841 cctatgaaac gctcgtcatg gatttaaaac agccccttgt tttgacaaac aacgccgata
     3901 aattcgatgt caacgccacc gttaaaggcg gcggtttcac cggtcagtcc ggcgcgatcc
     3961 gccttggtat taccagagcc ttattagagg ccggtgccga tcgcaccacg ctcaaggtcg
     4021 ccggtttcat cactcgtgat gcccgcacca aagaacggaa gaaatacggt cttaaaggcg
     4081 ctcgtcgcgc tccgcagttc tcgaagcgtt aagcggctgc gaaaatac
//
LOCUS       DAOSSL010000050         3966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_20507_length_3966_cov_12.086167, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000050 DAOSSL010000000
VERSION     DAOSSL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20507_length_3966_cov_12.086167"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..837
                     /locus_tag="PLH17_04455"
     CDS             301..837
                     /locus_tag="PLH17_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21051.1"
                     /translation="MRILLKILMVLVGIAAFFGIAASAAIWAYGQYGVPLVEEQIEAA
                     EDQVEASLEEEHPGTAITVDIQDVFYKFEGWAPSVAFEVYSLVEVELGGVDIEEKTSY
                     IVIDVMSVVSGGGEFETYEESEWDTAGASFKAAPRILFDGVEAKKVGLTWLIISTAAF
                     VGSIVVNAVFLRKRRKLV"
     gene            931..1552
                     /locus_tag="PLH17_04460"
                     /pseudo
     CDS             931..1552
                     /locus_tag="PLH17_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014939558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            1668..2765
                     /locus_tag="PLH17_04465"
     CDS             1668..2765
                     /locus_tag="PLH17_04465"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOR21052.1"
                     /translation="MITKTLFPGRYIQGYRAIERLQSELVSFGQNGICIVTRTTWQRL
                     RKCKLFQEIIPAIALCNFNGECCDEELNRLAEEGRKQKAHFVVAIGGGKAIDTGKIVA
                     SRLNLPVAVVPTIASTDAPCSACAVVYDATGAVLRVEYQKNNPNLVLVDSQIISEAPV
                     RFLASGMGDALATWFEAESCRRNKATNEAGELGSLTAYSLARLCYDTILKYGLEAYKS
                     CHNNQVTPALEAVIEANTLLSGLGFESGGLAAAHSIHNGLSSLAKTHGFYHGEKVAFG
                     LLASLFLREETKRYIDEVFTFCESIRLPTTLEDIGLRNITREELIQIATRACQHGEFI
                     HHEGNDITPQVVIDLLLMADQEGKRRKSMRL"
     gene            complement(2838..3866)
                     /locus_tag="PLH17_04470"
     CDS             complement(2838..3866)
                     /locus_tag="PLH17_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOR21053.1"
                     /translation="MSLPLQIILTVIISAIVIALIVFERIYVKKHEETIKKGMLFVFF
                     LVSLIAFLAGAFGIVLIWNVNITVLLTQIWGDIVIFFTTSVPALIGTAVVVFVWILIL
                     QLSRIALKRIGDKPGPMQKRRKTISKVLLSIINYALAIISFIIILAIWGVNVLPALAG
                     LGIVGLVVGLGAQKFIQDLIAGFFIIFEHHYDVGDVIEVGGFKGTVVDIGLKTTRLRN
                     WRGEIKILNNGEVNTLVNHSKNPSVAVVLFAISYEEDIDRTTEVLKAVFPSFRKQYAD
                     VLIEDPEVSGVMNLGVSGVEMRVTVKTLNEQHYGIERALRKLIKETLDANNIEIPLPQ
                     IVVNKPRE"
BASE COUNT         1076 a          871 c          954 g         1065 t
ORIGIN      
        1 aaaacagcga attgttagca aaaacgacct aaataggtcc ttaatctcca tcgcgccatc
       61 gtcgataacg atgaagaata gattccagtg gctctcgaac aatttacttg tgccttggat
      121 aagacgcttc tcaatataaa taacaactat tctcaaggcg gaatataatg gattttttta
      181 tttgctattc tattgcgcgc agtttgtccg tattctaata tggaatcaat agattaattt
      241 tcattgggtg aatctattga atctgcgata cccactcatc ggaaaagcga gggcaaaaat
      301 atgagaattc ttttgaagat tttgatggtt ttagttggca tcgccgcttt tttcggcatc
      361 gccgcttcgg ccgctatttg ggcttatggc caatacggcg ttccgctagt tgaagagcag
      421 atcgaagccg ccgaagatca agttgaagca agccttgaag aggaacatcc cggcacggcg
      481 atcaccgtcg acattcagga tgttttctac aagttcgaag gttgggcgcc atccgtggcc
      541 tttgaagtct attcactcgt cgaagttgaa ctcggcggtg tcgatatcga ggagaagact
      601 tcctacatcg tcatcgatgt catgagcgtc gtttccggcg gtggcgaatt tgaaacttat
      661 gaggaaagcg aatgggacac ggccggcgcc agttttaaag ccgctccccg cattctcttt
      721 gacggcgtcg aagccaaaaa ggtcggcttg acctggctca ttatctcgac tgccgctttt
      781 gtcggcagca ttgtggtcaa tgccgtcttt ctaaggaaga gaagaaaact ggtttaatcc
      841 ttctatatcg cttgattaat agcatttgcg gcaaattgat ttcatcggtt ttgtttttta
      901 agttatcatt tttagtgggg gttgctcacg atgacggaaa aagaaaaaat gattaaaggt
      961 atgatatatg actcaagtga tgaagagctg gccatggagc ggaaccgcgc tcatagacaa
     1021 tgcgctcaat ttaataaaat tgaagatgac ggcgacccgc aaaaacgtca gataatcgat
     1081 cttttgattc ctgataatgg cggggtctat ttgcagggcc ccatcttctt tgattatggc
     1141 tggaatacga cctttggcca caggtgctat gccaattaca attttgtgcg ttctagatgt
     1201 atgtcccgtc accataggcg atgatgtcat gatggggtgc aatgtttcgc tcatgacgcc
     1261 aatccatccg cttctttttg aagaaaaaaa acagtatgtc aatgcgcatg gaaaatttac
     1321 cgacagagaa tacgctaagc caatcgtcat cgaagcgggc tgctggatcg ccagcaatgt
     1381 cgtcatctgc ggcggtgtca ccatcggaag cgaatcggtc atcggggccg gctcagtcgt
     1441 cactaaggac attccgcgcg gcgtccttgc cgccggaaat ccatgccggg taattcgaaa
     1501 aattacggcc gaagactcaa tctattgcca aaaggattta ctccagagat gaagataaga
     1561 gcgagatgtc aaatctacgc tcttttttta ctctttgagt tgatacaata aaactaataa
     1621 gagatttgta tccacatagt tttgtttttg atataggggg acgaaagatg ataacaaaaa
     1681 cactttttcc cgggcgttat attcagggct atcgagcgat tgaacgcctt caatcagaac
     1741 ttgtctcgtt tgggcaaaat ggcatctgca tcgtgacgcg aacgacatgg caaagattgc
     1801 gaaaatgtaa gcttttccaa gaaatcatac ccgcgattgc cctttgtaat tttaatggcg
     1861 agtgttgtga tgaagaatta aaccgcctcg ccgaagaagg gcgaaaacaa aaagcgcatt
     1921 tcgtcgtagc tatcggcggc ggaaaggcca tcgataccgg aaagattgtg gccagtcgcc
     1981 tgaatcttcc cgtcgctgtc gtgccgacaa tcgcatcaac ggacgctcca tgcagcgcct
     2041 gcgctgtcgt ctatgatgct acgggcgcgg ttcttcgcgt cgaatatcaa aagaataatc
     2101 caaatctcgt cttggtggat tcgcaaatca tatccgaggc cccggttcgc tttctcgctt
     2161 cgggaatggg cgatgctttg gcgacgtggt ttgaagctga gtcgtgccgc cgcaacaagg
     2221 caactaatga agctggtgaa ttaggatcat tgacggccta ttctctcgcc cggctttgct
     2281 atgacacaat ccttaaatat ggtctggaag cttacaaatc atgccataac aatcaggtga
     2341 cgcccgcctt ggaagcggtt attgaggcga atacgctttt gagtggtttg ggttttgaaa
     2401 gcggcggcct tgcggctgca cattcgattc ataatggttt gtcatctctt gccaaaacgc
     2461 atggctttta tcacggtgaa aaggtggctt ttggcctctt ggcgtcactt tttttgcgtg
     2521 aggaaaccaa acgatatatc gatgaagtct ttaccttttg tgaaagcatc aggcttccga
     2581 cgactttgga agatatcggt ttaagaaata tcactcgcga ggaattaatt cagatcgcca
     2641 cccgcgcatg tcagcatggc gaattcatcc accatgaagg aaacgatatc accccgcaag
     2701 ttgtcatcga cttgctgctt atggcagatc aagaggggaa aagacgaaaa agcatgcgcc
     2761 tctgattgtt cgaaaatacc acctgacaat taaaaatagc cctgttttgc ggggctattt
     2821 ttgaaggact cgcagactta ttctcttggt ttattgacga caatttgcgg cagcggtatt
     2881 tcgatattat tagcatctag cgtctctttt atcaacttgc gaagagcgcg ttcgattcca
     2941 taatgctgct cattaagagt tttgacggtg acgcgcatct cgacgccgct cacgcccaga
     3001 ttcatgactc cagatacttc aggatcttct ataagaacat ccgcatattg cttgcgaaaa
     3061 gatggaaaaa ccgcctttaa aacctcggtc gtccgatcga tgtcttcttc gtaggaaatg
     3121 gcaaataaga cgacggccac agacggattc tttgagtggt tgacgagggt gttgacttca
     3181 ccgttattaa ggattttgat ttcgcctcgc caattgcgaa ggcgcgtcgt ttttaggccg
     3241 atatcgacga ccgtcccttt aaatccgccg acttcgatga catctccgac atcatagtga
     3301 tgttcgaaga tgatgaagaa accggcgatt aaatcctgaa taaatttctg agcgcctaaa
     3361 ccgacgacga gaccgacgat gccaagcccc gctaatgccg gcaagacatt gacgccccaa
     3421 atggcgagga taatgataaa gctgatgatg gcgagagcgt aattaatgat gcttaataat
     3481 accttggaga ttgttttgcg gcgcttttgc atcggccctg gcttatcgcc aattcgtttg
     3541 agggcgatac gcgagagctg caaaattaga atccagacga agacgacgac tgccgttccg
     3601 atcagcgcgg gcaccgaagt ggtgaagaag atgacgatat cgccccagat ttgagtgagt
     3661 aaaaccgtga tgttgacatt ccatattaat acgatgccaa aggccccggc gagaaatgcg
     3721 atgagcgaga cgagaaaaaa gacaaaaagc atccctttct taattgtctc ctcatgtttt
     3781 ttaacataga ttctttcaaa gacgatcaaa gcgattacga tggccgaaat tatgaccgtg
     3841 agaataattt gtaatggtag actcatatag tacctcccaa agcataaaga ttataacata
     3901 aaaaccattt tttggtgttg gctaacgcgt ttagactttt ccaattagta ggcattaagg
     3961 cggaac
//
LOCUS       DAOSSL010000051         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_21302_length_3849_cov_13.707433, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000051 DAOSSL010000000
VERSION     DAOSSL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21302_length_3849_cov_13.707433"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PLH17_04475"
     CDS             complement(<1..1552)
                     /locus_tag="PLH17_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOR21054.1"
                     /translation="MKKALNLAVITLAVLLNGLIVFESCLPGGVSVTRSNWLSNIFAD
                     IINVFIPGRVEVVPLESISLVGDQNIIIGTTNRFSVEYVPSNATSKGITFIAGNERIN
                     PVQEGSSCFVEGMELGSTSLMAVSLEDNSISKTVDITVNERPAPTEFELTVTNSQILN
                     GFSERVIFSIGDYTDNRAVRYFDTSLVPLESSDPDIATIEDGVIHAKSVGSVTIFASD
                     YPSRSVDIEVIENPNPLVYPDESSWEITGNNVAHIYDMDHAETNFNQLSIDWGEAIPS
                     DQSVTWKVEDELLAKIDADGKLRGYKKTGFTNVIAISNMDTEQQKIFPISIEEVLPTA
                     MDVNIRFDGSPLDEEVSVGDTIYISAIFSPDNTTNLYIDVISSDAAVLAAQIEGRTGK
                     ITAKKEGTATITVSSTANPELSETLVFEVMPKKAIDGENYVDFASFIRKSIGHFLLFG
                     VSAIFTTWAFYLTLNKVFKKKWFIVLVSGLFGITVASVTELIQLFIPLRSGTWIDVGI
                     NILGYAVFL"
     gene            complement(1650..2030)
                     /locus_tag="PLH17_04480"
     CDS             complement(1650..2030)
                     /locus_tag="PLH17_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21055.1"
                     /translation="MKMNVTKKEFAWYVVSGILVFIGVVLIIFDIIGDNIRVSTADNW
                     ILTAQNAVIEWSKINMDWRAWGIIFFLAGALVAVITLSVFAKGADRIVEKEQRRKARL
                     AAIKENGGSEDNVIEVEAKEVESK"
     gene            complement(2091..2939)
                     /locus_tag="PLH17_04485"
     CDS             complement(2091..2939)
                     /locus_tag="PLH17_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21056.1"
                     /translation="MENIQKAIVTLLHYNTIIRDTLEYTIKKKEYNLDFYNFKKRGVL
                     VEIEQNTPLKIFLDKAGENGQKLTTRIKDFYDTIYGDDSTILRVAPEGLRVDHAQHLT
                     VYEQVIILHEEIRRIANVHVDFAKKNNQFDQRLEDLIKADERFYRGLVYMTMMNDLEV
                     LYFDYNKVRNEAKGEITPQSNFIQNDISKVTNLMNFTRQNSVATDLAYMDLVDKVFAV
                     IENISGKRDLPPGKTYVDVFKEAKEAIANFVKDTEFKWRDLYNPVIKELLQASKNTGE
                     NQAAKA"
     gene            complement(2944..3777)
                     /locus_tag="PLH17_04490"
     CDS             complement(2944..3777)
                     /locus_tag="PLH17_04490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOR21057.1"
                     /translation="MKKIIATDLDGTLFYPKRRVRMISSRNKAFIRQYIDEGGKVIIV
                     SGRNYFFSHKVVSVIDRPVDVLSCNSAYISCDGKIIRELFLPAERMPQILEEINRDYD
                     PHGYLIMSKNQNLVTYDRYKSKLKNLANRFYYMIQGAYREPCVNSQEVFYDELSKGQV
                     YKLMIYFGLSKKAQKIAKEVNKELREKYPEIEASWIGMFIEITPRGCTKADGIKFYLD
                     YLKRSYQDVVVVGDSGNDISMFTAFEHSFCMEHAHKSVQKYAKHCIRHFSDLEQYVRE
                     E"
BASE COUNT          992 a          850 c          887 g         1120 t
ORIGIN      
        1 ccaagaaaac agcataaccg aggatgttga tgccgacatc gatccaggtt ccgcttctca
       61 gcggaataaa aagttgaatc agttcggtaa cgcttgctac cgtgatgcca aaaagaccgc
      121 tgacaagaac aatgaaccat ttcttcttaa acactttatt taacgttaaa taaaaagccc
      181 aggttgtaaa aatggcgctg acaccaaaca gcaagaaatg accgattgat ttgcggataa
      241 aagaggcgaa atcaacataa ttctcgccgt cgatcgcctt ttttggcatc acttcaaaca
      301 ccagggtctc gctcagttcg ggattggctg tggatgagac ggttatcgtc gctgtccctt
      361 cttttttggc ggtgattttt ccggttcttc cttcaatctg ggccgcgaga acagctgcat
      421 cgctcgagat cacatcgatg tataaatttg tcgtattatc aggcgagaaa atcgcgctga
      481 tgtagatggt gtcgcccacc gaaacttcct catcgagggg gctgccatcg aatctgatat
      541 tgacatccat tgccgtcggc agcacctcct cgatgctaat cggaaagatt ttctgctgct
      601 cggtgtccat attgctgatg gcgatgacat tggtgaaacc ggttttctta tatcctctca
      661 acttcccatc agcatcgatt tttgcaagca actcatcttc gactttccaa gtgacgcttt
      721 ggtccgaagg aatcgcttcg ccccaatcga tgctcagctg gttaaagttc gtctcggcgt
      781 ggtccatatc atatatatga gcgacattat taccggtaat ttcccaggat gattcatccg
      841 gataaactag tggattcggg ttttcaataa cttcgatgtc gaccgagcgc gaaggataat
      901 ctgaggcgaa gatagtgacg ctgcccacgc ttttagcgtg tatcacaccg tcttcgatgg
      961 ttgcgatatc cggatcggat gattcgagag gaactaagga ggtatcaaag tagcgaacgg
     1021 cgcgattatc ggtgtaatca ccaatcgaaa aaatgactcg ttcactgaac ccgttaagaa
     1081 tttgactatt ggtaacagtc agctcgaatt cggtcggggc agggcgctcg tttacagtga
     1141 tgtcaaccgt tttagagatg ctgttatctt ccaaggaaac ggccatcaag ctagtactgc
     1201 ctaattccat tccttcgaca aagcaagacg aaccttcctg gacgggatta atccgttcat
     1261 taccggcgat aaaagtgatg cccttgctgg tggcgttgga cggcacatat tcgacgctga
     1321 agcgattcgt agtgccgatg ataatatttt gatcgccgac gagcgagatt gattccaaag
     1381 gaactacttc gactcttccc ggaataaaga cgttgatgat gtcggcgaag atattgctca
     1441 gccagttgct gcgcgttacc gatactccgc cgggcaggca tgattcaaag acgatcagtc
     1501 cattgagtaa aaccgccaag gtgataacgg cgaggttcag cgctttcttc atgtgtctat
     1561 tttagaatat aaagacgggt ttttcattat ccaaaaactc ttagccatta ttttgctaat
     1621 catggtgaat aacataaaaa aggaatccgt tatttggatt ccacttcttt agcttcgact
     1681 tcgatgacgt tatcttcgct tccgccgttt tctttaatcg cggccagacg cgctttccgg
     1741 cgctgctctt tttcgacgat gcggtcagct cctttggcaa acaccgataa ggtgatgacg
     1801 gccacaaggg cgcccgcaag gaagaagatg ataccccagg cccgccagtc catattgatt
     1861 ttggaccatt cgatgacggc attctgggcc gttaaaatcc agttgtcagc ggtgctgacg
     1921 cggatgttgt cgccgatgat atcaaagatg atgaggacaa cgccgataaa aacgaggatg
     1981 ccggagacga cataccaggc gaattctttt tttgtgacat tcattttcat aatttagcct
     2041 acctattcta acatgtaaga atttgttttc tcaataacaa ttaagtgaga ttaagccttc
     2101 gcggcttgat tctcaccggt gtttttcgat gcttggagta actctttaat gactgggtta
     2161 tagagatccc gccatttaaa ttcagtatct ttgacaaaat tggcgatggc ctccttcgct
     2221 tctttgaaga catcgacata ggtttttcca ggcggaagat cgcgcttgcc cgagatattt
     2281 tcgatgacgg cgaatacttt atcgacgaga tccatataag cgaggtcggt cgccacggaa
     2341 ttctgacgcg taaaattcat caaatttgtc accttgctga tgtcattttg gatgaaatta
     2401 gattggggcg taatttcccc ttttgcttca tttctgactt tattgtaatc gaagtagaga
     2461 acttctaaat cgttcatcat cgtcatgtag actaagccgc gataaaagcg ctcatcggct
     2521 ttaattaagt cttcgaggcg ctgatcgaac tgattattct ttttggcaaa atcgacgtgg
     2581 acattagcga tgcggcgaat ttcttcgtga aggatgatga cttgctcata aactgtgagg
     2641 tgctgggcgt gatcaacgcg aagaccttcg ggagcgacgc gcaatatcgt cgaatcgtcg
     2701 ccgtaaatcg tatcgtaaaa atctttgatc cgggtcgtca gtttttgacc gttttcaccg
     2761 gctttgtcca aaaagatttt taagggtgtg ttttgttcga tttcgactaa gacgccgcgc
     2821 tttttgaagt tataaaaatc aagattatat tcctttttct tgattgtata ctccaaggta
     2881 tcacgaataa tcgtgttgta atgaagtagg gtgacgatcg ctttctgaat gttttccatg
     2941 tgtttattcc tctctcacat attgttccaa gtcactaaag tgacggatgc agtgctttgc
     3001 atatttttgc accgacttat gcgcgtgctc catgcagaag ctgtgttcga aggccgtaaa
     3061 cattgagata tcattcccgg agtcgccgac cacaacgaca tcctgataac tgcgcttgag
     3121 atagtcgaga tagaatttaa tgccatcggc tttcgtgcag ccacgcgggg ttatttcgat
     3181 gaacatgcca atccagctcg cctcgatttc cggatatttt tcgcggagtt ctttgttgac
     3241 ctctttagct attttctgcg cttttttaga aaggccgaaa tatatcatca gtttatagac
     3301 ctgccctttg ctaagttcgt catagaagac ttcctggctg ttgacacaag gttcgcgata
     3361 agcgccctga atcatatagt agaagcggtt ggccagattc ttcaatttgc tcttatagcg
     3421 atcatatgtc acgaggtttt gatttttgct catgatcaag tagccgtgag gatcgtaatc
     3481 gcgattgatc tcttcgagaa tctgcggcat gcgctcggca ggtaaaaaca actcgcggat
     3541 aatttttcca tcacaggata tataagcgga gttgcagctt aaaacatcaa cgggacgatc
     3601 gataaccgag acgactttgt gcgagaaaaa ataattgcgc cccgagacga tgatgacttt
     3661 gccgccttca tcgatgtatt ggcggataaa ggctttgttg cgcgacgaaa tcatgcggac
     3721 gcggcgtttg ggataaaaga gggtaccgtc taaatcggtg gcaataatct ttttcattcg
     3781 caagtatttt atactttacc tttttattaa acaagccata atgctttttt tctataagaa
     3841 aagaggcac
//
LOCUS       DAOSSL010000052         3738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_22124_length_3738_cov_12.448819, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000052 DAOSSL010000000
VERSION     DAOSSL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22124_length_3738_cov_12.448819"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..2500)
                     /locus_tag="PLH17_04495"
     CDS             complement(119..2500)
                     /locus_tag="PLH17_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOR21058.1"
                     /translation="MKIAWLNNIPEEDLKEVGGKAKGLFLLNQLGLNVARGFVAYDIS
                     TPEDETTLYEYWRDSGLGEVAVRSSATLEDGVELSSAGQYETYTCVTGEEQFKAAVKG
                     CIKSLNQVNASKYRQFFNQGQSSMNIVIQQMVKAKSAGVVFTINPTTRHNDVRIEVVA
                     GLGENLVSGKTQSQIILINREKPSFAIITTLLSNENIRQLFHDGLKIEEAMKVPVDLE
                     FAVDQNDCLVWLQARPITTLDDPLIGELNTPFAVEGDVITNHNVGEMLPGAITPLTIS
                     TVVYAIDYGLRKMLTDVGVVRNIEEVPAELLISHYYGHLFFNMRYLYRITKAVTGAAK
                     ENIDVGICGQILDYEQPDDFKRLNGFIRLRNGLRYGKFMAGYKKAMKDISSIADRIKF
                     SGETIDELYASITANLDLLNKALLDHYVTSVYSGAMNSALFYILARDDDNHDEIKGRI
                     ASVLEDIEDIESADIIKSLSAIAAAIIHDVQEAEDFDDNALKAYLKKAPTCVIELKDK
                     FMQRHGHRCIRESELRSPGWKDDEDGFVSHLLTLIKSMANYKQKEKKDGPNNVEIFLS
                     DYYGLKRLALRAFINGSRKGCRVREYSKSKMILAIDKFKDGYRRLAAMLVEQGALPEE
                     DLIYFLTHQELGEMLNTKDAKYVKKAFIRKRLLPEQERLKFAHVNVGAPEPISSTLDY
                     ASKGATLSGTPLSRGQVIGRARVVTNAEDAKKLQKGEIMVAGYTDIGWSPYYALIAGL
                     VTEVGSALSHGAVVAREYALPLVSNVKNATEVIRDGDLISIDGALGTVVIVDS"
     gene            complement(2510..3559)
                     /locus_tag="PLH17_04500"
     CDS             complement(2510..3559)
                     /locus_tag="PLH17_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21059.1"
                     /translation="MNIKKRIKKHFMINVLKSSSRKHPFSIEAADSYVLKDGVGANMT
                     DSHYFSGHASDGTSFFFRHAKRSDQTTEIWFCLKTSDGKVLCNKEELYPSSSCPADVR
                     CTISGEEMSFSFNGQVAYAHRGDNDILSLNKNGLNIRAKATFIASMPPFDFSTGLNPA
                     FVAGALAEEKWNKTFWRNLKQNRQVHYEQPGIIKLEATIENQTYTYVLPAMRDHSYGY
                     RDWNYMNRHIWLMVLFEDGEVLNLNMVDYPHMRNLMTGYISSTGNHTSLCSLTNLKKL
                     GQDGIVPQQIEIEIPLMDNHVATLIGNRELVADFSFADGKYHICEGVGNYAIGEKKGR
                     GILEFGYNRDTSRWK"
BASE COUNT          965 a          824 c          877 g         1072 t
ORIGIN      
        1 tccctttact gctagtaaag aaacaaaaga tcaagcccgc gctaaatttt tctaaaatta
       61 acggttgcgc aaacaaaaaa gtggatttcg gtccactttt taaattatta agctcttctc
      121 aggaatcgac gataacaact gttccgaggg cgccatcgat ggagatgagg tcgccatcgc
      181 ggattacttc ggtggcgttc ttgacgttag agacgagcgg taatgcatac tctcgggcga
      241 cgacggcacc atgtgagaga gcgctgccaa cttcggtgac gagaccggcg ataagcgcat
      301 aataaggtga ccaaccgata tcggtatagc cggcgaccat aatctcgcct ttttgcagtt
      361 ttttagcgtc ttcggcattg gtaacaacgc gagctcgacc gatgacttgt ccgcgtgaaa
      421 gcggtgtccc tgaaagagtc gcgcctttgc tggcataatc caaagtcgac gaaatcggtt
      481 ctggcgcgcc gacattgaca tgggcaaatt ttagccgttc ttgctcgggc aggagacgtt
      541 tacgaataaa ggctttctta acgtattttg catcttttgt attgagcatc tcgcccagct
      601 cctgatgtgt taagaaatat atcaagtctt cttccggaag agcgccctgc tcgacgagca
      661 tagcggctag gcggcgatag ccgtctttga atttatcgat ggccaaaatc atcttcgatt
      721 tagaatactc acgaacgcga catcccttgc gggaaccgtt gatgaaagcg cgaagagcaa
      781 gtctttttaa tccataatag tcagacagaa agatttcgac attgttggga ccgtctttct
      841 tctctttttg tttgtagtta gccattgatt tgataagggt gagcaggtgt gagacaaaac
      901 catcctcatc atctttccag cccggtgagc gaagctcgga ttcgcgaatg cagcggtggc
      961 cgtgtctttg catgaatttg tcttttagtt cgatgacaca agtcggggct tttttaagat
     1021 atgcttttaa agcattatca tcgaaatcct cagcttcttg aacatcatga ataatggcgg
     1081 cggcgatagc gctgagcgat ttgatgatgt cggcgctttc gatgtcttcg atgtcttcta
     1141 agactgaagc gatgcgacct ttgatttcat cgtggttgtc atcatcgcgg gccaaaatat
     1201 aaaatagagc gctattcatc gctcccgaat agacagaagt gacatagtga tccagcaagg
     1261 ctttgtttaa aagatcgaga ttcgcggtta ttgaagcata taattcatca attgtttcgc
     1321 cgctgaactt gatgcgatcg gcgattgatg agatgtcttt catcgccttt ttatagccag
     1381 ccatgaactt gccgtagcga aggccgttac gaaggcggat aaagccgtta agtcttttaa
     1441 aatcatccgg ttgctcgtaa tcaagtattt gcccacagat gccgacatcg atattttctt
     1501 tggcggcgcc cgtcaccgcc ttggtgatgc ggtagagata gcgcatgtta aagaagagat
     1561 ggccataata atggctgatg agcaactccg caggtacttc ttcaatattt ctgacgacgc
     1621 cgacatcggt cagcattttg cggagaccgt aatcgatggc atagacgact gtggaaattg
     1681 taagcggcgt gatagctcca ggcaacattt ctccgacgtt gtgattagta attacatcgc
     1741 cttcgactgc aaacggagta ttaagctcgc cgatgagcgg atcatcaagg gtggtgatgg
     1801 gtcgggcctg gagccagacg aggcagtcgt tttggtcgac ggcaaattca agatcgaccg
     1861 gaactttcat cgcctcttcg atttttaagc catcgtgaaa taactgacga atattttcgt
     1921 tgctgagaag ggtggtaatg atggcgaaag atggcttttc ccgattgatt aaaataattt
     1981 gtgactgcgt ttttccggag acgagatttt cgccgagacc agccactacc tcaatgcgca
     2041 catcgttgtg ccgagttgtc ggattgatgg tgaagacgac gccggctgat tttgctttga
     2101 ccatctgctg aataacgata ttcatcgaac tttgaccctg attgaaaaac tggcggtatt
     2161 tggaagcgtt gacttgattg agactcttaa tacagccttt gacagctgct ttgaattgct
     2221 cttcgccggt tacgcaggta tatgtttcat attggccggc agaggataat tcaacgccat
     2281 cttcaagcgt tgccgaagac cgcaccgcga cctcgcctaa accagaatca cgccaatact
     2341 cataaagagt cgtttcatct tccggcgtcg atatgtcata agccacaaag ccgcgagcga
     2401 catttaatcc gagctgattg agcagaaaaa ggcctttggc cttgcctcca acctctttta
     2461 gatcttcttc aggtatattg ttaagccatg ctattttcat ctttgcccct tacttccatc
     2521 gcgatgtatc gcggttataa ccgaattcaa ggatgccccg tccttttttt tcgccgatag
     2581 cataatttcc gactccttcg caaatatgat attttccgtc agcaaatgag aaatcagcaa
     2641 ccagctcacg atttccgata agtgtggcaa catgattatc cattaaggga atctctattt
     2701 ctatttgctg agggacgatg ccatcttgtc ccaacttctt taaatttgtc agggagcaaa
     2761 gcgaggtatg attacctgtg ctactaatgt atccggtcat caaatttcgc atgtgcggat
     2821 aatcaaccat gttgagattg agaacttcgc catcctcaaa aagaaccatc agccagatgt
     2881 ggcggttcat ataattccag tcgcgatagc cataggaatg atcgcgcatg gcgggcagaa
     2941 catatgtgta agtttgattc tcgattgtcg cctcgagttt aatgattccg ggttgttcat
     3001 aatggacttg gcggttttgc ttgagatttc gccaaaaagt tttgttccat ttttcctcgg
     3061 ctagggctcc ggcgacaaaa gccggattca gaccagtcga aaaatcaaat ggcggcatcg
     3121 aagcgataaa agttgctttc gcccgaatat taaggccatt tttattcaaa gataagatat
     3181 cattgtcgcc tcggtgtgca tacgctactt gcccattaaa agaaaacgac atttcttcac
     3241 cggatatcgt gcatctgaca tcggcgggac aggatgatga aggatatagt tcctctttat
     3301 tgcataagac ttttccgtca ctcgttttaa ggcagaacca aatctcggtc gtctgatcgc
     3361 tgcgcttggc gtggcgaaag aaaaaacttg tcccatcact ggcgtggccc gagaagtaat
     3421 ggctgtcggt catatttgct ccaacgccat ctttaagaac gtatgaatca gcggcttcta
     3481 tcgaaaatgg atgcttgcgc gaactcgatt tcaagacatt gatcatgaag tgcttcttta
     3541 ttctcttttt aatgttcata ttggatagtt ttgggcgttt tacactttgc ctattttagc
     3601 aaaagggaaa gaataagaaa actatgtttt tcctgacaat aagatggctg atttcctcac
     3661 gcaatcagga aacgttattt taatgtcgtt taacctgcag atagcagcgt caattattgt
     3721 ataatcataa tgtattgt
//
LOCUS       DAOSSL010000053         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_22567_length_3683_cov_21.525083, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000053 DAOSSL010000000
VERSION     DAOSSL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22567_length_3683_cov_21.525083"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1014
                     /locus_tag="PLH17_04505"
     CDS             22..1014
                     /locus_tag="PLH17_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOR21060.1"
                     /translation="MLTNKHVNKIFLSFALGAFLLSSCGKPSDTSSPVDTSSDTSSEI
                     SSDTSDNISSDTSNNTASDTSNDTSSDTSSGTSNSSSSSDNHYDMPSEYASYYSSLGS
                     NPTSSQLKSIVSSHTKLSYDGARSAMVKTDRDWTLSPNNSDSNPYMRLIYAAYNFKTS
                     SAARFNDNNTIWDREHIWAKSHGGFDNNATPGSDLHHLRASDKPNNNSWRNSRNFGNA
                     TSGQYGKDYRGLNSGKYSGGSLDGIYEPLDHDKGDVARACFYMAVVYNTNCTLVNSYP
                     GTGKTLGHLSTLLQWHELDPVDEFEMNRNNLIYDNYQHNRNPFIDHPEWAHSIF"
     gene            complement(1057..1632)
                     /locus_tag="PLH17_04510"
     CDS             complement(1057..1632)
                     /locus_tag="PLH17_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21061.1"
                     /translation="MKNGNDHKLKRIWTAVLLVLSWILCLVAVNYPSDHVMGYYPVLM
                     YVMYSLMAACSIAALAFILLKKPLMSDVLSFAVLACYFVMFGIVLVRRLLEGYVMFLP
                     MMAISLFILAGVSLMTVVLLLTGKKREGYLLAIIAFSFFALSVILESYPVAYDQVSEI
                     YGNIKLVYFVAIIAASVLGIINSAQMYRENK"
     gene            1782..2246
                     /locus_tag="PLH17_04515"
     CDS             1782..2246
                     /locus_tag="PLH17_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOR21062.1"
                     /translation="MPIESIMIWIWLGIFVLSVIIEAVEPDLVSIWFAAGALLAIILS
                     IIPGVPFWVEIIVFLVVSFVLIFAIRPLAKKYLYKKEIKSNIEEKIGEKCVVIKAINE
                     LAHGEVKLNGVIWTAIARNKKEVFEEQSVVTIVGIDGNKLIVEKFETGKEEK"
     gene            2248..3177
                     /locus_tag="PLH17_04520"
     CDS             2248..3177
                     /locus_tag="PLH17_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOR21063.1"
                     /translation="MQPWAIVVLSIVGAIVLLILVSSIKIVRQTDKYVIERLGGYLTT
                     WGVGIHMLIPFFDRISQRVTMKEQVKDFDPQPVITKDNVTMQIDTVVFFQVTDPKLYT
                     YGVINPISAIENLSATTLRNIIGDLDLDETLTSRDIINAKMRSILDEATDAWGIKVNR
                     VEVKNILPPKDIRDAMERQMRAERERREKILLAEGQKRSAILVAEGDKESVILNAEAQ
                     KQSAIRHAEGEAQAILEVKKAEAEGIKLIREAEAAGLEMLKNAKVDQAVLTLKSYEAL
                     AKVADGQSTKIIVPSDLQNVASLATAVAEIVKK"
     gene            3336..>3683
                     /locus_tag="PLH17_04525"
     CDS             3336..>3683
                     /locus_tag="PLH17_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21064.1"
                     /translation="MNRSTPSFRRLFSFTLLVFLLVSCGGKAESDFDVLKKEGEASGE
                     EIPVGTYDSKNIVFAYKNADSPFSATDLYVALDYSGESVQSRYALLDIEEYCYFLPEL
                     GTGRFQSSTVTFEY"
BASE COUNT         1074 a          903 c          796 g          910 t
ORIGIN      
        1 gcctttgcta taatcttaaa catgctcacc aataaacatg taaacaaaat atttctttcc
       61 ttcgctctcg gagctttttt gctctcgtct tgtggcaaac catctgacac ctcaagccca
      121 gtcgacacta gctctgacac gtcttccgaa atctcaagcg acacaagcga taacatctca
      181 tctgacacta gtaataacac tgcatccgat actagcaacg atacttcatc agacacaagc
      241 agcggcacat caaattcatc ttcttccagc gacaatcatt acgacatgcc ttctgagtac
      301 gccagctact atagtagttt aggaagcaat cccacgagct cgcaactcaa atcaatcgtc
      361 agcagccaca ccaaactatc ttatgacggt gcgcgcagcg ccatggtcaa gactgatcgc
      421 gactggactc tttcgccaaa taattccgat tccaacccct atatgcgcct tatttatgcg
      481 gcttacaatt tcaaaacctc aagcgccgcc cgctttaatg acaacaacac catttgggat
      541 cgtgagcata tctgggccaa atcgcacggc ggatttgaca acaatgccac tccggggtcg
      601 gatcttcatc atctccgcgc ctctgataaa ccaaataata acagctggag aaattcccgt
      661 aatttcggaa acgctaccag cggccaatat ggcaaagact atcgcggact taattccggt
      721 aagtatagcg gtggtagtct ggatggtatc tacgaacctt tagaccacga taaaggcgat
      781 gtcgccagag cctgcttcta catggcggtc gtctacaata cgaactgcac cttggtcaat
      841 tcgtatcccg gcaccggaaa gaccctcggg catctcagca cgcttttgca atggcacgaa
      901 ctcgatcccg tcgatgaatt tgaaatgaac cgcaacaatc tcatctatga caattatcag
      961 cacaaccgaa atcccttcat cgatcacccc gaatgggcgc actcgatctt ctaattcatt
     1021 cgttattatt gaatatcaac caccattagg tggtttttat ttgttttctc tatacatctg
     1081 cgcggaattg ataatgccca gcaccgatgc ggcgatgatt gcgacaaagt agactaattt
     1141 aatgtttcca tatatttccg acacttggtc gtaggcgacg ggataagact ctaggatgac
     1201 gcttaaagca aagaaggaga aggcgataat cgccaataag taaccctctc tttttttgcc
     1261 ggttaatagt aaaacaacgg tcataaggga gacgccggct aaaataaaaa gcgagatggc
     1321 catcatcgga agaaacatga cataaccttc gagcaaacgc cggacgagaa cgatgccaaa
     1381 catgacgaag taacaggcga ggacggcgaa agacaagacg tcgctcataa gcggtttttt
     1441 tagtaggata aatgccaacg cggcaattga gcaggccgcc atcaaagaat acatgacata
     1501 catcaatacg ggatagtagc ccattacgtg gtcgcttgga taattgacgg cgacgaggca
     1561 aagaatccaa ctcaggacca aaagaacggc ggtccagatt ctttttaatt tatgatcgtt
     1621 gccgtttttc attcatgact tcctcttatt aattatatga taatattcgc tttcaccgaa
     1681 acgattattg gcttgcatat atgcaaaaac ccttgaagaa tcctgtatta taattgttaa
     1741 tacgcgcgat ataatgtata ttttttatgg aggttatcaa tatgccaatc gaaagtatca
     1801 tgatctggat ttggctgggg atttttgtct tgtccgtcat catcgaagcg gtggaacccg
     1861 accttgtctc catctggttc gcggcgggag ctttgctggc cattattctg tcgattatcc
     1921 ccggagtgcc gttctgggtg gaaatcatcg tctttttagt cgtttcgttt gtcttgatct
     1981 tcgcgattcg tccgttggcc aagaaatact tgtacaagaa ggaaatcaaa tcgaatatcg
     2041 aagaaaaaat cggcgagaaa tgcgtcgtca tcaaagccat caatgagtta gcgcacgggg
     2101 aagtgaaact caacggggtg atttggacgg ccatcgccag aaataaaaag gaagtattcg
     2161 aagaacaatc agtcgtgaca atcgtcggta tcgacggcaa caaactgatc gttgaaaaat
     2221 tcgaaaccgg aaaggaagaa aaataatatg caaccttggg ctattgtcgt tttatcgatc
     2281 gtcggagcaa tcgtactcct cattctcgtc agcagtatta aaatcgtccg tcaaacggac
     2341 aaatacgtca tcgaaagact cggtggttat ttaaccacat ggggtgttgg catccacatg
     2401 ctgattccct tctttgatcg catctcgcaa cgcgtgacga tgaaagaaca agtcaaagac
     2461 tttgatcccc aacccgtcat caccaaagat aacgtgacga tgcaaatcga caccgtcgtc
     2521 ttttttcagg tcaccgaccc caaactatat acgtacggag ttatcaatcc gatttcggcg
     2581 attgaaaacc tctccgcgac gactctccgt aatatcatcg gcgatttaga tctcgatgaa
     2641 acactgacat caagagacat catcaacgcc aagatgcgta gcatccttga cgaagccacc
     2701 gatgcctggg gcatcaaagt gaaccgcgtc gaagttaaaa acatcctacc tccaaaggat
     2761 attcgcgacg ccatggaacg ccaaatgcgc gccgagcgcg aacgccgcga aaagattttg
     2821 ctcgccgaag gtcagaagcg gtccgcgatt ctcgtcgccg aaggcgataa ggaatccgtc
     2881 attctcaacg ccgaagccca gaagcaatct gccatccgcc acgcggaagg tgaagcgcaa
     2941 gccattctcg aagttaaaaa agccgaagct gaaggtatca aattaattcg cgaagccgaa
     3001 gccgccggtc tcgagatgct taaaaatgcg aaagtcgacc aagccgtcct gacgcttaag
     3061 tcctatgagg ctcttgctaa agtcgccgat ggtcagtcga cgaagatcat cgtccccagc
     3121 gatttgcaaa acgtcgcctc attggcgacc gccgtggctg aaatcgtcaa aaaataagtt
     3181 aattctgaaa gcgccgcgtg aaatttattt cacgtcctcg acaccgaggc ggcgcttttt
     3241 cttatgctta tgcttaatgt aaaaaaatat tattatgcga ggattaagta aagtattata
     3301 aaagcaggta aatataacaa aaaggaatca ataacatgaa cagaagcact ccttcatttc
     3361 ggcgcctgtt ttcgtttacg ctattagttt ttttgctcgt cagttgcggc ggcaaggcgg
     3421 aaagcgattt tgacgttctc aaaaaggaag gcgaagccag cggcgaagaa attcccgtcg
     3481 gcacttacga ttccaaaaat atcgtctttg cctacaaaaa tgccgatagc cccttcagcg
     3541 cgaccgacct atatgtcgct cttgattata gcggtgaaag cgttcagagc cggtacgcac
     3601 ttctcgatat tgaggaatat tgctatttcc ttccggaatt aggaacgggc cgctttcagt
     3661 cctcgaccgt cacctttgaa tac
//
LOCUS       DAOSSL010000054         3447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_24602_length_3447_cov_19.691038, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000054 DAOSSL010000000
VERSION     DAOSSL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24602_length_3447_cov_19.691038"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..901)
                     /locus_tag="PLH17_04530"
     CDS             complement(107..901)
                     /locus_tag="PLH17_04530"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOR21065.1"
                     /translation="MEKPDIKIVFTDIDMTLYSHRSKKVPESAIEAIKKLQAKGVLVF
                     LCSGRNYYLIRKTGILDIVEPDGLITMNGANAIIGDQIIYKYPIPENVVDSIIKFAKR
                     LKFGLTLIEEKEGHINFIDDRVISAHEKFGTRFPQPRSFPDHYDRVVYQMIAFCDELD
                     ESLFLPHLDNCKSARWDEFAVDIMPKDSDKAKGIMAVLEHYGWKPENALSLGDNPNDV
                     DMLLFTGTSIAMGNAVPQVKAVADYVTDDIDEDGWANAMKHYGLID"
     gene            1199..2095
                     /locus_tag="PLH17_04535"
     CDS             1199..2095
                     /locus_tag="PLH17_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOR21066.1"
                     /translation="MRQAVLVYNLVHETNREWAEQIDAFVVAFKKCGFELLPVSSVDA
                     YDYIKRYKRYINFVLYWDKDLYLAELLEATGVPVFNTSTAIRVCDDKALTYIKLKNHR
                     LPTPKTLVLPFTLGQNVIKFYDRIKTLIAEKGFAYPFVLKQRFGSFGEQVYLITSEVM
                     FKKMLQTIGDKELLIQEFIEESSGRDFRVNVVGREAVSIVMRVNQGNFRSNVHQGGIM
                     SSVPHPDRRMLKLAVQAAKAVGADFAGVDILVNAAGEPLVIEVNSNARTIAVEEASKV
                     YVSLFIARYIKKHYKLRKLDLY"
     gene            2121..3422
                     /locus_tag="PLH17_04540"
     CDS             2121..3422
                     /locus_tag="PLH17_04540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOR21067.1"
                     /translation="MNITVIGQGYVGLVNSVILASFFHNVIGLDNDKNKVATLKQGIA
                     TIKEPNLQSLMSDAKDRLRFTTNAKDAIRPNKIVYVAVDTPEGKDGKPDMTNFNACLD
                     DIAKNAIQDTYVVIRSTVPVGTNARAKKYLEENSEHKFEVISNPEFLSQGSAIDDMLN
                     PSRVVIGVTSKAGEDLMLQMYANYQKRKIPILITTPESAELIKYASNNFLAMKISYIN
                     EIARLCEMVDADIEEVAIGIGLDPRIGRNYLKAGIGYGGSCFPKDTKALSWLAMDREV
                     PLELVRATIKVNNEQPAILMHKINSHFRSLSNLTIAVLGVAFKAGTEDIRNSPATAVV
                     KYLLEKNAYVKVYDAMAIENFQKEMQRHSRIEYSTSILDTLKDADAVVILTDAKELRE
                     LDRSCFIDNMKKPVIFDGRNLYKPANMAGTTYYSIGRRTIK"
BASE COUNT          948 a          857 c          782 g          860 t
ORIGIN      
        1 aacgtaaaaa tctttagtcc ttgttttatg agcgacccgc ctgttcttgc gtaatttttt
       61 gtaaaaatca gatataaaaa aagtgcataa ctgcacttgc ttttacttag tcgatgagac
      121 cgtaatgttt catggcgttt gcccatccgt cttcgtcgat gtcgtcggtg acgtagtcgg
      181 cgacggcttt gacttggggc acggcattac ccatcgcgat ggacgtgccg gtaaacagaa
      241 gcatgtcgac atcattggga ttatcaccaa gcgataaagc attttccggt ttccagccgt
      301 aatgttccaa gacagccata attcctttgg ccttgtccga atctttcggc atgatatcga
      361 cggcgaattc atcccagcgc gcgctcttgc aattatcaag atgcggcagg aagagtgatt
      421 catcgagttc atcgcaaaaa gcaatcatct gatagacgac gcggtcgtag tgatcgggaa
      481 acgaacgcgg ttgcggaaag cgggttccga atttttcgtg agcgctgatg acgcggtcat
      541 caataaaatt gatgtgacct tctttttctt caatgagagt caagccgaac tttaagcgct
      601 tggcgaactt gataattgaa tcgacgacat tttcgggaat cgggtacttg tagatgatct
      661 gatcgccgat gatggcgttc gcgccgttca tggtgatgag gccgtcaggt tcgactatgt
      721 cgagaattcc ggtcttgcga atcagatagt agttgcggcc cgaacacaaa aagacgagaa
      781 caccctttgc ttgaagtttt ttaatggcct caatcgcact ctccggcact tttttgctcc
      841 gatgcgaata aagtgtcata tcgatatccg taaaaacaat tttgatatct ggtttttcca
      901 ttgctgctcc tttcttttta tttcgagtta gctcgttact cttcaggcgg attacactcc
      961 ttagtcacca ggtgcctaga aaccttctta agaagctccg cattattata aaagaaacac
     1021 ggctattagt caaaatcaat caccatctgg caatacctat gatacttgcc gatggaagta
     1081 aagcgctatt tatttcgttc ccatcggcat atttttgtct taaacccctc ttataaggca
     1141 gcgatacaat ttgcaattgt gacacaaata aggcacaata tacactaagg ttataaccat
     1201 gagacaagca gtcctcgtct acaatctcgt ccacgagacg aatcgcgaat gggccgagca
     1261 aatcgacgcc tttgtcgtcg cttttaaaaa gtgcggtttc gaactccttc cggtctctag
     1321 cgtcgacgcc tatgactaca tcaaacgcta taagcgctat attaattttg tcttgtactg
     1381 ggataaagat ttgtacttgg ccgagctgct cgaagcgacc ggcgtaccag tcttcaatac
     1441 gagcacggca attcgcgtct gcgacgataa agccctgacc tatatcaagt tgaaaaacca
     1501 tcgcttgccc actcctaaaa cgctggtgct gcccttcacc ttagggcaga atgtcattaa
     1561 gttttatgat cgcatcaaga cgttaatcgc cgaaaaagga tttgcctatc cgttcgtctt
     1621 gaagcagaga tttggctcct ttggcgaaca agtctacctg atcaccagcg aagtaatgtt
     1681 taaaaagatg ctccagacta ttggcgataa ggaactttta attcaagaat tcatcgaaga
     1741 gagctccggt cgcgactttc gcgtcaatgt cgtcggacgg gaagccgtct caatcgtcat
     1801 gcgcgtcaat caaggcaatt tccgcagcaa cgtccatcaa gggggcatca tgtcgtcagt
     1861 acctcatccc gaccgccgca tgctcaagct ggcggtgcag gccgcaaaag cggtgggcgc
     1921 cgacttcgcc ggcgtcgaca tcctcgtcaa tgccgcgggc gagccgctgg tcatcgaagt
     1981 caactcgaat gcccgcacga tagccgtcga agaagccagc aaagtctacg tctcgctttt
     2041 tatcgcccgg tacatcaaaa aacactataa actccgcaaa ttagacttgt attaactgat
     2101 aaattcgaaa ggaggcctct atgaacatca cagtcatcgg ccaaggatat gttggacttg
     2161 tcaatagcgt gatcctcgcc tctttttttc acaacgtgat tggcctggat aacgacaaga
     2221 acaaagttgc gaccttaaaa cagggcatcg cgacgattaa agaaccaaat ttgcaatcgc
     2281 tcatgagcga cgcgaaagac cgccttcgtt ttacgacgaa cgccaaagac gcgattcgcc
     2341 ccaataaaat cgtctatgtc gccgtcgata ctccggaagg caaagacgga aaaccggata
     2401 tgacgaattt taacgcttgc cttgacgata ttgctaaaaa cgccattcag gatacgtatg
     2461 tcgtcatccg gtcgacggta ccggtcggca cgaatgcccg cgccaaaaaa tatctcgaag
     2521 agaatagcga gcacaaattt gaagtcatct cgaatcccga attcttaagc cagggaagcg
     2581 cgattgatga catgctcaac ccttctcgcg tcgtcatcgg cgtgacgagt aaagccggcg
     2641 aagatttaat gctgcaaatg tatgcaaatt atcagaagcg caagattccg attttgatta
     2701 caacaccgga aagcgccgag ttaatcaaat atgcctccaa taattttttg gcgatgaaaa
     2761 tcagttacat caacgaaatc gctcgtttat gcgagatggt ggatgccgac atcgaagaag
     2821 tcgcgattgg tatcggcctt gatccccgca tcggtcgcaa ctatttaaaa gccggcatcg
     2881 gctatggcgg ctcttgcttt cctaaagaca cgaaagcttt aagctggctg gcgatggacc
     2941 gcgaagtacc gctcgaattg gtgcgggcca caatcaaagt caataacgaa caacccgcca
     3001 tcctgatgca taaaatcaac tcccatttcc gcagtctttc gaatttaaca attgccgttc
     3061 tcggcgtggc gttcaaggcc gggaccgaag atatccgcaa ctcaccggca acggccgtcg
     3121 tcaagtatct gctcgagaaa aacgcctatg tcaaagtcta cgatgccatg gcgatcgaaa
     3181 acttccagaa agagatgcag cgccactccc gcatcgagta ttcgaccagt attctcgata
     3241 ccttaaaaga tgctgatgca gtcgttattc tgaccgacgc caaagaactc agagaattag
     3301 accgcagctg cttcatcgac aacatgaaaa agcccgtcat ctttgatggc cgcaaccttt
     3361 ataagccggc taacatggcg gggacgacct actattccat cggccgcaga acaatcaaat
     3421 aagcgacaaa aaaaccgagg attcgac
//
LOCUS       DAOSSL010000055         3103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_28360_length_3103_cov_19.638123, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000055 DAOSSL010000000
VERSION     DAOSSL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28360_length_3103_cov_19.638123"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLH17_04545"
     CDS             complement(<1..730)
                     /locus_tag="PLH17_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21068.1"
                     /translation="MTNKFSRYLRSKKKLIRALIDQLGTRYSYVSVLGTDVSGTTIVV
                     DKSTANVVPSGLSEAGFVIKVFNGQIYSELSTNEITKKNIPILISEINRLAAHKSTIA
                     PVKVKGLNEEPLTKRFVRKNKGQSYTVEEIISILKGYVTRTLKGSELAVNVRASIETT
                     EVSKMYMSKQKDLEQYYTWANPRCYVLVRRGDNTKYGYDGFGINSIEKALEDLLPAID
                     KSLKTAIELLDSVIPTPGYYDVITA"
     gene            1042..2736
                     /locus_tag="PLH17_04550"
     CDS             1042..2736
                     /locus_tag="PLH17_04550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOR21069.1"
                     /translation="MKLEQFPLIIPNIDAIKQEFETLLQAFEAAPTAADQKNVIYDIA
                     KLVDEVTNQLTLISVRYTQDTRVEAYEKAQEASDEITPLFQELYNRYQKALIASKFRS
                     ELEKMLGSFLFKKIELSLQAFDPKIIGELQQENKLTSQYAKALASAQIPFKGGVYNLS
                     QMGKFLDEADRYTRKKASQAIAGWFGEHEEELAKIYDDLVHLRHQMARKLGFDNFIPL
                     GYARMGRTDYSPLDVKSYRDQVYKAVVPLSKKLVRRQAERIGIKKPLFYDLSLNFATG
                     NPTPKGDKDYLVGVAAEMYAEMSPDIGAFFKLMVERHMLDLESRPGKQGGGYMTYFPK
                     YKIPFVFSNFNGTSGDIDVLTHEIGHAFQGYSSRHIKLPEYRDPTMEEAEIHSMSMEF
                     FAWPWMERFFKEDKDKYQYAHLVSAINFLPYGVTVDEFQHFVYENPDSGHAARCAKWR
                     EIEKKYTPYKKYAGFPLYEKGARWMRQSHIFEAPFYYIDYTLAQVVAFQFLTLMEEDR
                     ATAWKRYVKLCKLGGSYPFTELLKRVKMKNPFKKGTISRTVKKLEPIIARFDDAKM"
BASE COUNT          847 a          742 c          708 g          806 t
ORIGIN      
        1 gcgcggtgat cacatcgtaa tatccgggcg tcggtataac cgaatcaagt aattcaattg
       61 cggttttaag cgacttatcg atcgccggaa gcaaatcttc aagagctttc tcaatcgagt
      121 taatgccaaa accgtcgtaa ccgtatttgg tgttgtcacc acggcgaacc aatacataac
      181 agcggggatt ggcccaagta taatactgct ccaaatcctt ttgctttgac atatacattt
      241 ttgagacttc ggtcgtctcg atcgatgccc ggacattaac ggcaagttcc gaacccttga
      301 gggtcctcgt tacatatcct ttaagaatgc tgataatttc ttcaaccgta taagattggc
      361 ctttgttttt acggacaaaa cgttttgtga gcggctcttc gttcaatcct ttgactttga
      421 cgggcgcgat tgtgctctta tgagcggcga ggcggttgat ttcggaaatc aggatgggga
      481 tgttcttctt cgtaatctcg ttcgtcgaga gttcgctata aatttggccg ttaaaaactt
      541 tgatgacaaa gcccgcttcg ctgagaccgc tgggaacgac attggccgtc gacttatcga
      601 cgacaatggt cgtgccggaa acatccgtgc ccaaaacgct gacataggaa tatcgggttc
      661 cgagttgatc gattaaagcg cgaatcagct tctttttact tctgagataa cgggaaaatt
      721 tgttcgtcat actgctacct tcaattttgc tattgtagca caaaacgtga ttttatatca
      781 cgattaggat aaattgtaac cgcaatatgc aaattacgac gaataaatga tggacaagac
      841 atgtaattaa tccaccgcaa tttatatttt tatcccgatt tagtatttta gattgtcgta
      901 aagaaagtat ccgcttgcgg tgccacctac gcatggtgaa tatgccgtgc tataatgaca
      961 aagtcatcaa ggattggcgt ccattcacgg tcggcatgac ctcatccgtg tcgttcagta
     1021 tcaattaaaa ggagaacaac catgaaatta gaacaattcc cgttaattat tcccaacatc
     1081 gatgccatca agcaggagtt tgaaacactc ttgcaggcgt ttgaggccgc cccgacagcc
     1141 gccgatcaga aaaatgttat ctatgatatc gccaaattgg tcgacgaggt gacaaaccaa
     1201 ctgacgctta tcagcgtccg ctacactcaa gacacacgcg ttgaggccta tgagaaagct
     1261 caggaagcca gtgacgagat aactcctctt ttccaggaat tatacaatcg ctatcagaag
     1321 gccctcatcg cctcaaaatt tcggtcggag cttgaaaaga tgctcgggtc attcttattt
     1381 aagaaaatcg aactgagcct ccaagctttc gatccgaaga ttatcggcga gctgcaacag
     1441 gaaaataaac tgacaagtca atacgccaag gcgctggcct cggcccaaat tcccttcaaa
     1501 ggaggcgtct acaacctgtc gcagatgggt aagttcctcg atgaagccga ccgctatact
     1561 cgcaaaaagg cgtctcaggc tatcgctggc tggtttggtg agcacgaaga ggaattggcc
     1621 aaaatctacg acgatttggt gcatttgcgt catcagatgg cccgtaaact cggctttgat
     1681 aatttcatcc cgctaggcta tgcccgcatg gggcggaccg attacagtcc tctggatgtc
     1741 aaaagctacc gcgaccaggt ttacaaagcc gtggtcccgc tctccaagaa attagtgaga
     1801 cgacaagccg aacgaatcgg catcaaaaaa cctcttttct atgatttgag ccttaacttt
     1861 gccaccggta acccgacgcc aaaaggcgat aaggattacc tcgtcggagt agccgcagag
     1921 atgtatgcgg agatgagccc cgacatcggc gccttcttca agctgatggt cgaacggcac
     1981 atgctcgacc ttgaatcccg tcccggcaag caaggcggcg gctacatgac ctattttccg
     2041 aaatacaaga ttccgttcgt attttcaaac ttcaacggca cttcgggcga catcgacgtc
     2101 ctgacccacg aaataggaca cgccttccaa ggctacagtt cgcgccacat caagttgccc
     2161 gagtaccgcg acccgacgat ggaagaagct gaaatccatt cgatgagcat ggaatttttc
     2221 gcttggccgt ggatggagcg gttcttcaaa gaagataagg ataaatatca atatgcgcac
     2281 ctcgtcagcg ccattaactt cctgccctat ggcgtcaccg tcgatgaatt tcagcatttc
     2341 gtttacgaaa atccggattc cgggcatgcc gcgcgctgcg ccaagtggcg tgaaattgaa
     2401 aagaagtaca ccccgtataa gaaatacgcg ggatttccgc tttacgagaa aggggcgcgc
     2461 tggatgcggc aaagtcacat ctttgaggca ccattctatt acatcgacta caccttggcc
     2521 caggtcgtcg cctttcagtt cctgacgctt atggaagaag acagagcaac tgcgtggaaa
     2581 cgctatgtca agttgtgcaa gctcggcggc tcttatccgt tcaccgaact tctgaagaga
     2641 gtcaaaatga aaaacccctt taaaaaaggt acgatttccc gcacggtcaa gaaactcgaa
     2701 cccatcattg cgcgtttcga tgacgcgaag atgtgattaa gtcgatgctc ggcgtcacat
     2761 ttatatgatc tttatcatgc tcatcggtat ccgcatcata tggatgtcaa tgtgattgct
     2821 gagaacgccg atgtagcaaa gtaatcaaca aaaaaggcgc gagctgggtg tgtctttttt
     2881 tcttttaaaa ttagagcgag tctgtgcttt tcttcctttt taaaaaacac ctcattttta
     2941 ttaaaaatgt ttactaaaaa taccctacaa ttcacttttc ctcttgtgta taatgggttc
     3001 atgaaaaaga atagtatcat tatggcatta attggtttat gtctatcgtc aacggcgtgc
     3061 tccgcaaaca agacgccggt cgatgtcgtg cttatggccg gcc
//
LOCUS       DAOSSL010000056         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_31736_length_2855_cov_16.710000, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000056 DAOSSL010000000
VERSION     DAOSSL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31736_length_2855_cov_16.710000"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLH17_04555"
     CDS             <1..556
                     /locus_tag="PLH17_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21070.1"
                     /translation="HHRQMKAGVIALINDLQAIGYDVKESELPDNDFWTSLDVARILV
                     KKGYASRIWTAAFDEIIHKAEHRRIFFCDPKEIIDMVHECGGLAFAAHPFDIIEDDMK
                     IRVSKEVVDEMLPFLVEAGIDGVEGCYQPYSLEMREYSLEFAKKNNLLISYGTDFHGG
                     RPYEEAKYIEIAKAPDWIIKLISK"
     gene            complement(581..1018)
                     /locus_tag="PLH17_04560"
     CDS             complement(581..1018)
                     /locus_tag="PLH17_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21071.1"
                     /translation="MSFTLSRQLKDCQKADAVIVFEFVKDLGVEVLSLPSSEESLKIN
                     LESLAPYRVSYEALKNQEGKSQIVISLSTIVVDLAQRKRLLSYALTEGLKVAKEKLLK
                     ILAVNVDDNLLDEYREIDVIEVATKAIKTFIKKSDMNVTLCIE"
     gene            1391..2158
                     /locus_tag="PLH17_04565"
     CDS             1391..2158
                     /locus_tag="PLH17_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOR21072.1"
                     /translation="MNTIILGSLFDGPHLGYIATSLAITALLLWLPFRFIKSQKYKDI
                     YLKVWALATFFLHISPLWIAFLNGQPAIAADNVLFPIYFCNMSMYLLVVVAFWGNKKT
                     KFFGYISTVVAYAGAFGALISLFYPDYYLHGTTIFEWGVFKSMLSHSTMLIGSLYLFV
                     GGYFKVNRYNTIIYAIGLLFFGAVGLIVNGIFAAASLNAPNAMYLQHPPIEDVPFLNV
                     YVIAALMVLLVFAISYTYEVVSKKMSVLKKPVTQENH"
     gene            2260..2430
                     /locus_tag="PLH17_04570"
     CDS             2260..2430
                     /locus_tag="PLH17_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21073.1"
                     /translation="MKKRFIYIVFICLAIPVLYACNIDDTNSISDTVSSDSSSISDGG
                     ASSSSATSTTDI"
     gene            2548..>2855
                     /locus_tag="PLH17_04575"
     CDS             2548..>2855
                     /locus_tag="PLH17_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21074.1"
                     /translation="MNYHGSNLLENLKRGDIIFEATGFYGITGHVAIVEDIFYSETYE
                     QYYVRIIEAISVGVARSIITPTRMLQKEGSAYRIKDASEAQIDEVIEFVIDQLGKPYQ
                     V"
BASE COUNT          779 a          641 c          596 g          839 t
ORIGIN      
        1 gcaccaccgc caaatgaagg ccggtgtcat cgcgttgata aacgatttac aggcaattgg
       61 atacgatgtt aaagaatcgg agctacccga taatgatttc tggacgagtc ttgatgtcgc
      121 gcgcatcctt gtcaaaaaag gctacgccag cagaatatgg acagcagcct ttgatgagat
      181 tattcataaa gcagagcata gacgcatttt cttttgcgac ccgaaagaaa taatcgacat
      241 ggtccatgaa tgcggagggc tggcctttgc ggcccatcct ttcgatataa tcgaagatga
      301 tatgaaaata agggtgtcca aagaagtcgt ggacgaaatg ctcccgtttc tcgtcgaggc
      361 gggaatcgac ggagtcgaag gctgctacca accctatagc cttgagatgc gcgagtattc
      421 gcttgaattc gctaaaaaga ataatctgct gatttcatat ggaaccgact ttcacggcgg
      481 gcgtccttat gaggaagcta agtatatcga aatcgccaaa gcgcctgact ggattatcaa
      541 actaattagt aagtagtacg gggcgaggtt tactaactga ttattcgatg cagagagtga
      601 cattcatgtc gctcttttta ataaaggtct taatcgcttt cgtggcgact tcaatgacat
      661 caatctctcg atattcgtct aagagattgt cgtcaacatt gaccgcgagg attttaagca
      721 acttttcttt cgctactttc aatccttcag tcagggcata agacaagagg cgcttcctct
      781 gggcgaggtc aacaacgatt gtcgataaag atatcacaat ctggcttttt ccttcttgat
      841 tcttgagcgc ttcgtaactc acgcgatagg gcgctaaaga ttcaagatta atcttcaggc
      901 tctcttcgga cgaaggcaaa gataaaacct caactcctaa atctttgacg aactcgaaca
      961 caatcacggc atcggctttt tgacaatctt ttaactgtct tgataaggta aacgacattt
     1021 ccaatcctcc ttgggatgat gactagtgat ctaacggaga tgatgctccc tagatgcagg
     1081 acgagggcga gtaagagtgc cttctttttg tcttgcaagg tcattttaac atgctaaaaa
     1141 aatatttatt ctcaacatta taattactat gtaattatat ttgcgttagt tggtaataat
     1201 cgtcttaata tttcttgcta tatgttggca aacaacaata aacaggcctt acacgcgatt
     1261 gtaaataagc atcaaacggg cccggccgtc attattaccg ctttcatctt tcgccttcta
     1321 ttctattttt tgaatctttg ttaaaatact caaagcgtca gtcaagacga cttaataaat
     1381 gaggtatgtt atgaacacca tcatccttgg ctctttattc gatgggccgc acctgggcta
     1441 tatcgccaca tcgcttgcga taacggcttt gctattatgg ctgcccttcc gcttcatcaa
     1501 atcgcagaaa tacaaagata tatatttaaa ggtctgggcc ttagccacct tctttcttca
     1561 tatttctccg ctttggattg ccttccttaa tggccagccc gcgattgccg cggataatgt
     1621 tttgttcccc atctacttct gcaatatgtc gatgtatctt ttggtcgtcg tggcgttctg
     1681 gggcaacaag aaaacaaaat ttttcggata tatttcgact gtcgttgctt acgcgggcgc
     1741 atttggagct ttaatatcgc tattttatcc tgattattat cttcacggca cgaccatctt
     1801 cgaatggggc gtcttcaaaa gcatgctgag ccacagcacg atgctaatcg gatctttata
     1861 tctctttgtg ggtggctatt tcaaagtcaa tcgctacaac acgattatct atgcgatcgg
     1921 ccttctcttc ttcggagcgg ttggcctcat tgtcaacggc atctttgccg cggcgagttt
     1981 aaatgccccc aacgcgatgt atttgcagca tcctcccatc gaagatgtgc ctttcttaaa
     2041 cgtctatgtc attgccgctt taatggtcct cttggtattt gccatctcct atacttatga
     2101 ggtggtcagc aaaaagatga gcgtcttaaa aaaaccagta acgcaagaaa atcactgagg
     2161 agactatgct aatggctttc tttgtttagc tatttatata agtgtttatt catttgacgg
     2221 tgcaatgccg tcatttcttt tttataatca aaataacata tgaaaaaaag atttatctat
     2281 attgtcttta tctgccttgc cattcctgtg ctttacgcct gcaatattga tgatacaaat
     2341 tcgataagcg acaccgtcag tagcgattct tcatcaatca gtgatggcgg cgcctcgtct
     2401 tcttctgcca catcaaccac ggatatatag agcagtgatt cctcctctac tttcagcgaa
     2461 tatgaaggat tcgacggata ccaatatccc gtcgacgaaa acgaagaaga ttactggtac
     2521 gataccggaa tcgcgcttcc catcggcgtg aactatcatg gttccaactt attagaaaat
     2581 ctaaagcgcg gagacatcat ctttgaagcc accggctttt acggaataac cggccacgtc
     2641 gcgattgtcg aagacatctt ctattccgaa acatatgaac aatattacgt gcgcattatc
     2701 gaagccatca gcgtcggcgt cgcccgcagc atcataacgc cgacgcggat gcttcaaaaa
     2761 gaaggaagcg cctatcgcat taaagatgca agcgaagccc agattgatga ggtaatagaa
     2821 ttcgtaattg atcaactcgg aaagccttat caggt
//
LOCUS       DAOSSL010000057         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_43850_length_2252_cov_15.929904, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000057 DAOSSL010000000
VERSION     DAOSSL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43850_length_2252_cov_15.929904"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLH17_04580"
     CDS             <1..361
                     /locus_tag="PLH17_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21075.1"
                     /translation="TLTERLDILLDKYRYTAITVRLTGDPKVIYERFSYRDLNTNRHR
                     GHVVNDRYPEKPGSKKANPTRKTYEQYLKDIKERGFSTFASNGPVIDVDVTDLEKVDY
                     NLLNAKLKTIIKSHKAG"
     gene            610..1164
                     /locus_tag="PLH17_04585"
     CDS             610..1164
                     /locus_tag="PLH17_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOR21076.1"
                     /translation="MNALTFSKVEDFHNWPVLNHRDHAGIWIRFDKLNSDASIGPEQA
                     LDEALCFGWIDGQLKRLDSQYYVKYFAKRTPQSIWSTKNKASAERLIKEGKMMPSGLL
                     AVESAKKDGRWERADQIPSDFSIDGLQNLLCHDEKALRNFLKMSPSVQKTYALSYFAL
                     KRPESRERRLKIIIERLTNNLPPM"
     gene            complement(1227..2114)
                     /locus_tag="PLH17_04590"
     CDS             complement(1227..2114)
                     /locus_tag="PLH17_04590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOR21077.1"
                     /translation="MWLIYVIYQVAGDTKLLKRLSLPFATYFAANLVLVIVNLFFGYY
                     YTIDNSNVYARGPVYPLAVVWAFLPLLVSFVITLIKRRRIDSRKYSAFLFFPIAPIIG
                     TILSFFTYGYSIVLPSMAVGYLLLFISIQSDTMRVDYLTGVFNRRHLEDCLRRKMSEN
                     NPSFAAIMLDIDCYKKMNDTYGHLVGDRALVDFARVLRKAVGINDIVARYGGDEFMII
                     VAVNNINQLNKLVDEIREHLERFNAKKVYPFKLTASFGQAMYIPSEKKTMEQFINHVD
                     DLMYKNKKTNCLLDTTSTD"
BASE COUNT          705 a          471 c          527 g          549 t
ORIGIN      
        1 tacgttaact gaacgcctgg atatcttatt agataaatat cgttacacag cgatcaccgt
       61 gagactgaca ggcgatccta aagttatcta tgaaagattt tcttaccgag atttgaatac
      121 aaatagacat cgcggacatg tagttaatga ccgctatcca gaaaaaccgg gcagcaagaa
      181 agctaatccg acaagaaaga cttatgaaca atatcttaaa gatattaaag agagaggatt
      241 cagcactttt gcctcgaacg gtccggttat tgatgttgat gtcacagatt tagaaaaagt
      301 ggattacaac cttctgaacg caaaattaaa aacgatcata aaatcgcaca aagcgggttg
      361 aaaacggcga ataattagga aacgttactt aaacttccaa agaacctcgc cgccgcaggt
      421 cgcacttacc acgattggaa aaacaccatt tgaaatgtta aaagatggaa gaatttcgga
      481 aatgggttct accgcgaatg ctcgatatat taagatatag acaaaaaatc aaccgccatt
      541 attcggttct ccaagcggga ggacttctat tctggcgtaa gccaaccaat cagtaactga
      601 ggtaaaaaca tgaacgcatt aacattttca aaagtcgagg acttccataa ctggccggtt
      661 cttaatcatc gtgatcatgc cggcatctgg atccgttttg ataaattgaa cagcgatgcg
      721 tcaatcggcc ccgagcaagc attagatgag gctctctgct ttggctggat tgatggacag
      781 ctgaaacgac ttgattctca atactatgta aagtactttg ccaaaaggac gccccaaagc
      841 atctggtcga cgaaaaacaa agcctctgcc gaacgtttga taaaggaagg aaagatgatg
      901 ccgtccggcc ttctggcggt ggagagcgcc aagaaagacg ggcgctggga acgcgccgat
      961 caaattcctt cagacttttc aattgatggc ctccaaaacc tgctttgtca cgacgaaaag
     1021 gccttgcgca attttcttaa aatgtctcct tcggttcaaa agacttacgc tttgagttac
     1081 tttgctttga agcgacccga aagcagagag cggcgcctta aaattatcat tgaacgcctg
     1141 acgaacaatc ttcctccaat gtaaatagaa aaagttacta aacaataacc cggctcattt
     1201 gttcgctttt aatcggaagg caataattag tcagttgaag tcgtgtctaa caaacaattc
     1261 gtctttttat ttttatacat caaatcatcg acatggttga tgaattgctc catcgttttc
     1321 ttttcgctcg ggatgtacat cgcttgccca aaactcgccg tcagtttaaa cggatacact
     1381 tttttggcat tgaatctttc gaggtgctct ctgatttcgt cgactaactt attgagctga
     1441 ttgatattgt taaccgcgac gataatcatg aactcatcac cgccataacg ggcgacgata
     1501 tcgttgatgc cgaccgcttt gcgaagcact ctggcgaagt cgacgagagc ccggtcaccg
     1561 actaagtgac catatgtatc gttcattttt ttgtagcaat caatatcgag catgatggcg
     1621 gcgaaggacg gattgttttc gctcatcttt ctgcggaggc aatcttcgag gtggcggcgg
     1681 ttgaatacgc cggtcagata atcaacgcgc atcgtgtcgg attgaatgct gatgaaaagg
     1741 agcagatagc cgacggccat cgatggcagc acgattgaat agccataagt aaaaaatgac
     1801 aagatggtgc cgataatcgg ggcgatcggg aaaaataaga aggcgctata ttttcgcgaa
     1861 tcgatgcggc ggcgttttat caaagtgatg acaaaagaaa ccaaaagggg caaaaacgcc
     1921 caaacgaccg ccaagggata aaccggtccg cgagcataga cattactgtt atcaatcgta
     1981 tagtagtaac caaagaacaa gttgacgatg accaagacga gattggcggc aaaataagtc
     2041 gcgaaaggca aggatagtct ttttagtaac ttagtatcgc ccgccacctg atagatgaca
     2101 taaatgagcc acaagatggg agggagggga ttgagagcaa agagcaggaa attgccgatt
     2161 tgattggcga gggggaatgc taagactggt cagaccgtcc atgcggccga tgatatcaac
     2221 agcgaataat aggatgccgg aggagatgat ga
//
LOCUS       DAOSSL010000058         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_70533_length_1621_cov_14.297573, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000058 DAOSSL010000000
VERSION     DAOSSL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70533_length_1621_cov_14.297573"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLH17_04595"
     CDS             complement(<1..1004)
                     /locus_tag="PLH17_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOR21078.1"
                     /translation="MAYNGFGIDQYLDATLVTKQLDELIKKPVYSIKKEHLNDYVENY
                     FNKKCLKSKEMIGLAKEIIPGGVQHNLAFNYPFPIVVTKAEGAKLYDIDGNEYFDLLQ
                     AGGPTIIGSNDPAVRDKVIELLNTCGPSTGLFHEYEYKLAKKISEMFPSVERFRMLGS
                     GTEACMVAVRIARLRTKHKNILKMGGAYHGWSDQLAYGLRVPGTKGIMAKGIPGFVFK
                     HTKEFFPNDLKDLERKLRLNKLSGGTAAIFIEPVGPESGTRPLSKEFIQGTERLAKKY
                     GALLIFDEVVTGFRIGQSGAQGYFGVDPDLTIFGKIVAGGNARRLDKEGSFTRSNRQL
                     A"
     gene            complement(1188..>1621)
                     /locus_tag="PLH17_04600"
     CDS             complement(1188..>1621)
                     /locus_tag="PLH17_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOR21079.1"
                     /translation="FKGRVIAPGYFKGEAVVSHQGVNTLATFQKSALKNAKEVLVSDQ
                     NNPDIYEKNITGKALCLPITIGSTTGGLVIQTVCSMGINPACFLFSEHIDSLAASGIV
                     LARIWEESNVIAIDKLGKEFLDNVKTGDLLEVKEDGTVTIL"
BASE COUNT          390 a          359 c          405 g          467 t
ORIGIN      
        1 gccagctgtc gatttgaccg agtgaacgac ccttctttgt caaggcgacg agcattgccg
       61 ccagcgacga ttttcccaaa aatagtcaga tcgggatcga cgccgaaata accctgtgcg
      121 ccgctttgtc cgattcgaaa gccggtgacg acttcgtcga agatgagcaa agcgccgtat
      181 tttttggcaa ggcgttccgt gccttgaata aattcttttg ataacggccg cgttccgctt
      241 tccggtccga ccggttcgat gaagatcgcg gccgtgcccc cgcttaattt attaaggcgc
      301 aattttcttt ccaaatcttt taagtcattg gggaaaaatt ccttggtgtg tttgaaaaca
      361 aatccgggaa tgcctttggc catgatgccc tttgtgcccg gtacgcgcag gccataggct
      421 aattgatcgg accagccgtg ataggcgcca cccatcttaa ggatgttttt gtgctttgtt
      481 cttaggcggg cgatgcgaac ggccaccatg cacgcttccg ttccggaacc gagcatgcga
      541 aagcgttcca cggatggaaa catctcggaa atcttcttgg ccagtttata ttcgtattcg
      601 tgaaagagac cagttgaagg tccgcatgta tttagtaatt caattacttt atctctgacg
      661 gcggggtcgt tgctgccgat gatggtcggt ccgccggcct gaagcaagtc aaagtattca
      721 ttgccgtcga tatcgtagag ctttgcccct tcggctttgg tgacgacgat ggggaaaggg
      781 taattaaagg ctaagttgtg ctggacgccg ccgggaatga tttctttggc gaggccaatc
      841 atttccttag atttaaggca ttttttgtta aagtagtttt cgacatagtc gttgagatgc
      901 tctttcttga tggagtagac cggtttttta attaattcat ctaattgttt ggtgactaaa
      961 gttgcatcca ggtattgatc gatgccgaaa ccattatagg ccatgcttat ctccttatat
     1021 atctcgtgag tgattgctca cacctgacga tttaaaggta acatgccaaa aaatggctgt
     1081 caataagata tcaaaaaagc agccgcgaag atgtgcggct gcttcattaa tatacgtttg
     1141 tttgctaata atagcaaaat cggtaaaacg aacattaatc tatgacttta aaggatggtg
     1201 acggttccgt cttctttgac ttccaagaga tcaccggtct taacgttatc caaaaactct
     1261 tttccgagtt tatcgatggc gatgacattg ctctcctccc atatgcgggc gaggacgatg
     1321 ccgcttgcgg ccagactatc aatgtgttca ctaaatagga agcaagcggg attaataccc
     1381 atcgagcaga cggtctgaat gacgagaccg ccggttgtgg aaccaattgt gatcggtaag
     1441 cataaagctt tgccggtgat gttcttttcg tagatatcgg gattgttttg atcggagact
     1501 aaaacttctt tggcgttctt taaggcgctt ttctggaaag tcgccaaagt gttgacgccc
     1561 tggtgcgaga cgacggcttc gcctttaaag tagccgggag cgatgactct tcctttgaat
     1621 t
//
LOCUS       DAOSSL010000059         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_2242
            NODE_75155_length_1553_cov_15.946595, whole genome shotgun
            sequence.
ACCESSION   DAOSSL010000059 DAOSSL010000000
VERSION     DAOSSL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864309
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:30:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 923
            CDSs (total)                      :: 887
            Genes (coding)                    :: 881
            CDSs (with protein)               :: 881
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75155_length_1553_cov_15.946595"
                     /isolate="AMR_MDS_2242"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLH17_04605"
     CDS             <1..204
                     /locus_tag="PLH17_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOR21080.1"
                     /translation="ENTWTYSFVNVEAESEISFYFYHWSTVDYTIYADASSTLFAHTA
                     VGDATLEITGTFANPVGTGTMII"
     gene            complement(344..1219)
                     /locus_tag="PLH17_04610"
     CDS             complement(344..1219)
                     /locus_tag="PLH17_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOR21081.1"
                     /translation="MKQYILATDACSDLPLDFVKQHDIAIVPMILEIEGKSYSHNLEE
                     KDLKLKDFYNRLRDKKVSRTSLVNVGEFVQFFTGLLKEGKDILYIALSSALSGTYQSA
                     MMAIDMLKDEFPDQRIVAVDTLSASLGEGYLVWRAALLKKEGKLLNEVADWLEANKFR
                     LRHLFTVEELGTLKRGGRLSGTAAFFGTLLGVKPILHVTREGKLEVLEKVVGRKKSLT
                     QMIDVLKEQIVDSENQTVFIAHGDCLEEAKEVGGLINASIKVKDLFYSYIGPVIGSHS
                     GPGTIAVFFMGKERE"
     gene            complement(1344..>1553)
                     /locus_tag="PLH17_04615"
     CDS             complement(1344..>1553)
                     /locus_tag="PLH17_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOR21082.1"
                     /translation="PGAIGGFGANHHLRQALTFLNVATMQQPEAYIGSVHTLLDDQGK
                     VANEGTMKFLSQFANAFLKWIQANS"
BASE COUNT          373 a          357 c          384 g          439 t
ORIGIN      
        1 gaaaacacct ggacctattc ctttgtgaac gtggaagccg aaagtgaaat ttccttctat
       61 ttctatcatt ggagcactgt cgattacacc atctatgccg atgcttcgag caccctcttc
      121 gctcatactg ccgtgggcga tgcgacgctt gaaataaccg gaacattcgc taatcctgtc
      181 ggcacgggaa cgatgattat ttagttaatt tccaacatca taaggcgggt tcttcccgcc
      241 ttttattcag caaaaaggcg tcatcactga tggcgccttt tcatccagga tccctggatt
      301 tctctaatgc tgagtatact ggagttaact cttttgtaaa gaattattct ctttctttgc
      361 ccatgaagaa gacggcgatg gtgccgggtc ccgaatgcga gccgatcacg ggtccgatat
      421 aactataaaa caagtcttta actttgatgc tagcgttgat gagaccaccg acttccttgg
      481 cttcttcaag gcaatcgccg tgagcgataa acacggtctg attttccgaa tcgacaattt
      541 gctcttttaa gacatcgatc atctgcgtca gcgacttctt gcggccgacg actttttcta
      601 atacttcgag ttttccttcg cgggtgacgt gaaggatggg cttgacgccg agcaatgtgc
      661 cgaagaaagc cgcggttccg cttaagcggc ccccccgctt gagggtgcct aattcctcga
      721 cggtaaacaa gtgacggagg cggaatttgt tggcctcgag ccagtcggcg acttcattga
      781 ggagcttgcc ttctttctta agaagggcgg ctctccagac gagatagcct tcgccgagcg
      841 aggcgctgag agtgtcgacg gcgacgatac gctgatcagg aaattcgtct ttgagcatgt
      901 cgatggccat catggccgat tgataagtgc cgcttaaggc cgaggaaagg gcgatgtaga
      961 ggatgtcttt gccttccttg agaagtccgg tgaaaaactg gacgaattct ccgacattaa
     1021 caagcgaggt gcgcgatact tttttgtcgc gcagacggtt ataaaaatcc ttgagtttta
     1081 aatctttttc ttcgaggttg tgggaataac ttttaccttc aatctctaag atcatcggaa
     1141 cgattgcgat gtcgtgttgt ttaacaaaat caagaggcag gtcggaacag gcatcggtcg
     1201 ctagtatgta ctgtttcatt tgtttctcct ttataacttt tggtttcgtt atcttaatac
     1261 ataacaaaag tcttgtcatc tattttaatc aaagactcat agcgacaaga caattacggg
     1321 ttgttataaa aatgttttat cggttaggaa tttgcctgta tccactttaa gaaggcgttg
     1381 gcaaattggc tcagaaactt catagtgccc tcattggcga ctttcccctg atcatcgagc
     1441 aaggtgtgaa cgctgccgat ataggcttcc ggctgctgca tggtggcgac atttaaaaag
     1501 gtcagcgcct ggcgcaggtg atggttggct ccaaagccgc cgatggcgcc ggg
//
